title: Role of Base Excision Repair (BER) in Transcription-associated Mutagenesis of Nutritionally Stressed Nongrowing Bacillus subtilis Cell Subpopulations.
abstract: Compelling evidence points to transcriptional processes as important factors contributing to stationary-phase associated mutagenesis. However, it has not been documented whether or not base excision repair mechanisms play a role in modulating mutagenesis under conditions of transcriptional derepression. Here, we report on a flow cytometry-based methodology that employs a fluorescent reporter system to measure at single-cell level, the occurrence of transcription-associated mutations in nutritionally stressed B. subtilis cultures. Using this approach, we demonstrate that (i) high levels of transcription correlates with augmented mutation frequency, and (ii) mutation frequency is enhanced in nongrowing population cells deficient for deaminated (Ung, YwqL) and oxidized guanine (GO) excision repair, strongly suggesting that accumulation of spontaneous DNA lesions enhance transcription-associated mutagenesis.
authors.affiliation: Department of Biology, Division of Natural and Exact Sciences, University of Guanajuato, Guanajuato, Mexico.
authors.forename: Verónica
authors.initials: V
authors.lastname: Ambriz-Aviña
authors.affiliation: College of Arts and Sciences, University of Missouri-St Louis, St Louis, MO, USA.
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Yasbin
authors.affiliation: School of Life Sciences, University of Nevada, Las Vegas, NV, USA.
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Robleto
authors.affiliation: Department of Biology, Division of Natural and Exact Sciences, University of Guanajuato, Guanajuato, Mexico. pedrama@ugto.mx.
authors.forename: Mario
authors.initials: M
authors.lastname: Pedraza-Reyes
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair; D005434:Flow Cytometry; D016296:Mutagenesis; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins