title: Polygonatum sibiricum polysaccharide inhibits osteoporosis by promoting osteoblast formation and blocking osteoclastogenesis through Wnt/β-catenin signalling pathway.
abstract: Bone homeostasis is maintained by a balance between bone formation by osteoblasts and bone resorption by osteoclasts. Osteoporosis occurs when osteoclast activity surpasses osteoblast activity. Our previous studies showed the plant-derived natural polysaccharide (Polygonatum sibiricum polysaccharide or PSP) had significant anti-ovariectomy (OVX)-induced osteoporosis effects in vivo, but the mechanisms of PSP's anti-osteoporosis effect remains unclear. In this study, we assessed PSP's effect on the generation of osteoblast and osteoclast in vitro. This study showed that PSP promoted the osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) without affecting BMPs signaling pathway. This effect was due to the increased nuclear accumulation of β-catenin, resulting in a higher expression of osteoblast-related genes. Furthermore, the study showed PSP could inhibit the receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis and exert prophylatic protection against LPS-induced osteolysis in vivo. This effect was also related to the increased nuclear accumulation of β-catenin, resulting in the decreased expression of osteoclast-related genes. In conclusion, our results showed that PSP effectively promoted the osteogenic differentiation of mouse BMSCs and suppressed osteoclastogenesis; therefore, it could be used to treat osteoporosis.
authors.affiliation: Department of Spine Osteopathia, the First Affiliated Hospital of Guangxi Medical University, Nanning 530000, Guangxi, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Du
authors.affiliation: College of Public Hygiene of Guangxi Medical University, Nanning 530000, Guangxi, P. R. China.
authors.forename: Meng-Ni
authors.initials: MN
authors.lastname: Nong
authors.affiliation: Department of Osteopathia, the First Affiliated Hospital of Guangxi Medical University, Nanning 530000, Guangxi, P. R. China.|Research Centre for Regenerative Medicine and Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning 530000, Guangxi, P. R. China.
authors.forename: Jin-Min
authors.initials: JM
authors.lastname: Zhao
authors.affiliation: Department of Spine Osteopathia, the First Affiliated Hospital of Guangxi Medical University, Nanning 530000, Guangxi, P. R. China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Peng
authors.affiliation: Department of Spine Osteopathia, the First Affiliated Hospital of Guangxi Medical University, Nanning 530000, Guangxi, P. R. China.|Research Centre for Regenerative Medicine and Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning 530000, Guangxi, P. R. China.
authors.forename: Shao-Hui
authors.initials: SH
authors.lastname: Zong
authors.affiliation: College of Public Hygiene of Guangxi Medical University, Nanning 530000, Guangxi, P. R. China.
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism* / Q000473:pathology; D010010:Osteoclasts / Q000378:metabolism* / Q000473:pathology; D010024:Osteoporosis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D031384:Polygonatum / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D051176:beta Catenin