title: [Construction of Protein Phosphatase 2A Catalytic Subunit β (PPP2Cβ) Overexpression Lentiviral Vector and Its Effect on K562 Erythroid Differentiation].
abstract: OBJECTIVE
  To construct the ovexpression lentivirus vector of PPP2Cβ, the catalytic subunit of protein phosphatase 2A, so as to obtain high-titer packaged lentivirus particles, and to examine the effect of PPP2Cβ on the erythroid differentiation Methods: The CDS of PPP2Cβ was cloned into the second generation of lentivirus vector FUGW, which should be used to co-transfect HEK 293T cells with the lentiviral expression vector and packaging vectors including pMD2G and pSPAX2. Lentiviruses were harvested at 36 and 48 hours after transfection. Titers of viral stock were determined by using flow cytometric analysis. The Western blot was performed to detect the expression level of PPP2Cβ in K562 cells transinfected with the lentiviruses. Benzidine staining and real-time PCR analysis were used to assess the erythroid differentiation of K562 cells.
  
  
  RESULTS
  The PPP2Cβ overexpressing lentivirus vectors were constructed, the high-titer lentiviral particles were obtained, and then the PPP2Cβ overexpression K562 cell line was established and promote erythroid differentiation of K562 cells.
  
  
  CONCLUSIONS
  This study suggests that overexpression PPP2Cβ can promote K562 cell erythroid differentiation.
authors.affiliation: School of Postgraduate, Anhui Medical University, Hefei 230032, Anhui Province, China; Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Dong
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Zhan
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Yin
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Yang
authors.affiliation: School of Postgraduate, Anhui Medical University, Hefei 230032, Anhui Province, China; Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Chang-Yan
authors.initials: CY
authors.lastname: Li
mesh_terms: D002454:Cell Differentiation; D041905:Erythroid Cells; D005822:Genetic Vectors; D006801:Humans; D020014:K562 Cells; D016086:Lentivirus; D054648:Protein Phosphatase 2; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: C517190:PPP2CB protein, human; D054648:Protein Phosphatase 2