title: A novel Direct MIC-gradient Strip Method to screen for antibiotic-resistant faecal Enterobacteriaceae.
abstract: A novel method to detect resistant faecal Enterobacteriaceae was developed by applying MIC-gradient strips directly onto agar plates inoculated with faeces. The method provided the susceptibility pattern (MICs) of the dominant bacterial population directly on the plates and also detected smaller resistant subpopulations with a sensitivity of 1/10(5).
authors.affiliation: Department of Microbiology, Division of Laboratory Medicine, Oslo University Hospital, PB 4956 Nydalen, NO-0424 Oslo, Norway; Faculty of Medicine, University of Oslo, PB 1171 Blindern, NO-0318 Oslo, Norway.
authors.forename: Karianne Wiger
authors.initials: KW
authors.lastname: Gammelsrud
authors.affiliation: Department of Paediatric Medicine, Division of Paediatric and Adolescent Medicine, Oslo University Hospital, PB 4956 Nydalen, NO-0424 Oslo, Norway; Faculty of Medicine, University of Oslo, PB 1171 Blindern, NO-0318 Oslo, Norway. Electronic address: p.k.knudsen@medisin.uio.no.
authors.forename: Per Kristian
authors.initials: PK
authors.lastname: Knudsen
authors.affiliation: Norwegian Institute of Public Health, PB 4404 Nydalen, NO-0403 Oslo, Norway.
authors.forename: E Arne
authors.initials: EA
authors.lastname: Høiby
mesh_terms: D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000295:instrumentation*; D002648:Child; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiotic resistance surveillance; Direct faecal plating method; Faecal Enterobacteriaceae; MIC-gradient strip