title: The Colicin E1 TolC-Binding Conformer: Pillar or Pore Function of TolC in Colicin Import?
abstract: The mechanism by which the drug export protein TolC is utilized for import of the cytotoxin colicin E1 across the outer membrane and periplasmic space is addressed. Studies of the initial binding of colicin E1 with TolC, occlusion of membrane-incorporated TolC ion channels, and the structure underlying the colicin-TolC complex were based on the interactions with TolC of individual colicin translocation domain (T-domain) peptides from a set of 19 that span different segments of the T-domain. These studies led to identification of a short 20-residue segment 101-120, a "TolC box", located near the center of the colicin T-domain, which is necessary for binding of colicin to TolC. Omission of this segment eliminated the ability of the T-domain to occlude TolC channels and to co-elute with TolC on a size-exclusion column. Far-ultraviolet circular dichroism spectral and thermal stability analysis of the structure of T-domain peptides implies (i) a helical hairpin conformation of the T-domain, (ii) the overlap of the TolC-binding site with a hinge of the helical hairpin, and (iii) a TolC-dependent stage of colicin import in which a central segment of the T-domain in a helical hairpin conformation binds to the TolC entry port following initial binding to the BtuB receptor. These studies provide the first structure-based information about the interaction of colicin E1 with the unique TolC protein. The model inferred for binding of the T-domain to TolC implies reservations about the traditional model for colicin import in which TolC functions to provide a channel for translocation of the colicin in an unfolded state across the bacterial outer membrane and a large part of the periplasmic space.
authors.affiliation: Department of Biological Sciences, Purdue University , Hockmeyer Building of Structural Biology, West Lafayette, Indiana 47907, United States.
authors.forename: Stanislav D
authors.initials: SD
authors.lastname: Zakharov
authors.affiliation: Department of Biological Sciences, Purdue University , Hockmeyer Building of Structural Biology, West Lafayette, Indiana 47907, United States.
authors.forename: Xin S
authors.initials: XS
authors.lastname: Wang
authors.affiliation: Department of Biological Sciences, Purdue University , Hockmeyer Building of Structural Biology, West Lafayette, Indiana 47907, United States.
authors.forename: William A
authors.initials: WA
authors.lastname: Cramer
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D003087:Colicins / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D008051:Lipid Bilayers; D017433:Protein Structure, Secondary; D021381:Protein Transport; D013056:Spectrophotometry, Ultraviolet; D055672:Static Electricity
chemical_list: D003087:Colicins; D029968:Escherichia coli Proteins; D008051:Lipid Bilayers