title: Protective effect of treatment with thiamine or benfotiamine on liver oxidative damage in rat model of acute ethanol intoxication.
abstract: OBJECTIVE
  The aim of this study was to evaluate possible beneficial effects of treatment with thiamine or benfotiamine in an animal model of acute ethanol intoxication.
  
  
  METHODS
  Thirty male Wistar rats were separated at random into three groups of 10 animals each: Ethanol (E), Ethanol treated with thiamine (T) and Ethanol treated with benfotiamine (BE). Rats were gavaged with single dose of ethanol (5g/kg, 40% v:v). After 30min of ethanol gavage the animals were treated with thiamine or benfotiamine. Six hours after first gavage, the animals were euthanized and blood and liver samples were collected for ethanol and oxidative stress biomarkers quantification.
  
  
  RESULTS
  Serum ethanol levels were higher in animals treated with thiamine or benfotiamine while hepatic alcohol levels were higher in animals of the group treated with benfotiamine comparing to controls or thiamine treated groups. The lipid peroxidation biomarkers were diminished for the groups treated with thiamine or benfotiamine comparing to E animals. Concerning protein oxidative damage parameters, they were enhanced for animals treated with benfotiamine in relation to other groups.
  
  
  CONCLUSIONS
  In conclusion, the treatment with thiamine or benfotiamine even 30min after the massive dose of ethanol has proven to be beneficial against liver damage. Improved results were obtained with benfotiamine in relation to oxidative damage from aqueous compartments.
authors.affiliation: Department of Nutrition, Health Sciences Institute, Federal University of Triangulo Mineiro, Brazil. Electronic address: gvportari@nutricao.uftm.edu.br.
authors.forename: Guilherme Vannucchi
authors.initials: GV
authors.lastname: Portari
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil.
authors.forename: Paula Payão
authors.initials: PP
authors.lastname: Ovidio
authors.affiliation: Department of Physical Education, Faculty of Physical Education and Sport, State University of Londrina, Brazil.
authors.forename: Rafael
authors.initials: R
authors.lastname: Deminice
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil.
authors.forename: Alceu Afonso
authors.initials: AA
authors.lastname: Jordão
mesh_terms: D000435:Alcoholic Intoxication / Q000188:drug therapy*; D000818:Animals; D004195:Disease Models, Animal; D008107:Liver Diseases / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013831:Thiamine / Q000031:analogs & derivatives* / Q000627:therapeutic use*
chemical_list: D013831:Thiamine; C013835:benphothiamine
keywords: Acute alcohol intoxication; Benfotiamine; Oxidative stress; Thiamine