title: Adenosine A2a receptors form distinct oligomers in protein detergent complexes.
abstract: The human adenosine A2a receptor (A2aR) tunes its function by forming homo-oligomers and hetero-oligomers with other G protein-coupled receptors, but the biophysical characterization of these oligomeric species is limited. Here, we show that upon reconstitution into an optimized mixed micelle system, and purification via an antagonist affinity column, full-length A2aR exists as a distribution of oligomers. We isolated the dimer population from the other oligomers via size exclusion chromatography and showed that it is stable upon dilution, thus supporting the hypotheses that the A2aR dimer has a defined structure and function. This study presents a crucial enabling step to a detailed biophysical characterization of A2aR homodimers.
authors.affiliation: Department of Chemical Engineering, University of California Santa Barbara, CA, USA.
authors.forename: Nicole S
authors.initials: NS
authors.lastname: Schonenbach
authors.affiliation: Department of Chemical Engineering, University of California Santa Barbara, CA, USA.
authors.forename: Monica D
authors.initials: MD
authors.lastname: Rieth
authors.affiliation: Department of Chemical Engineering, University of California Santa Barbara, CA, USA.|Department of Chemistry and Biochemistry, University of California Santa Barbara, CA, USA.
authors.forename: Songi
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Chemical Engineering, University of California Santa Barbara, CA, USA. momalley@engineering.ucsb.edu.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: O'Malley
mesh_terms: D003902:Detergents / Q000737:chemistry*; D006801:Humans; D008823:Micelles; D055503:Protein Multimerization; D043705:Receptor, Adenosine A2A / Q000737:chemistry*
chemical_list: D003902:Detergents; D008823:Micelles; D043705:Receptor, Adenosine A2A
keywords: G protein-coupled receptor; dimer; ligand chromatography; oligomer; protein detergent complex; size exclusion chromatography