title: [Preliminary research on the expression of sclerostin mediated by bone morphogenetic protein 2 in cementoblast].
abstract: OBJECTIVE
  This research explores the regulatory role of bone morphogenetic protein 2 (BMP2) in the expression of sclerostin in OCCM-30 cementoblast.
  
  
  METHODS
  OCCM-30 cementoblasts were treated with 50 and 100 ng · mL⁻¹ BMP2 for 3, 5, and 7 days. SOST mRNA was detected by real-time quantitative polymerase chain reaction (RT-PCR). Western blot analysis was employed to detect the sclerostin levels in the nucleus. Five groups were prepared for the experiments: control, BMP2, BMP2+dorsomorphin, BMP2+SB202190, and BMP2+PD98059. OCCM-30 was pretreated with BMP2 for 3 and 5 days, and then the sclerostin and SOST mRNA levels were measured.
  
  
  RESULTS
  RT-PCR and Western blot analyses showed that BMP2 upregulated the expression of SOST in a concentration-dependent manner. SOST expression increased with time (P < 0.05). Moreover, sclerostin levels of BMP2+dorsomorphin, BMP2+SB202190, and BMP2+PD98059 groups were lower than that of the BMP2 group, and the sclerostin level in BMP2+dorsomorphin group was lowest (P < 0.05).
  
  
  CONCLUSIONS
  The upregulation of SOST by BMP2 in OCCM-30 is mainly mediated by the BMP2/Smad signal pathway.
authors.forename: Chen
authors.initials: C
authors.lastname: Yue
authors.forename: Li
authors.initials: L
authors.lastname: Shuqin
authors.forename: Huang
authors.initials: H
authors.lastname: Lan
authors.forename: Dai
authors.initials: D
authors.lastname: Hongwei
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D015153:Blotting, Western; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D003739:Dental Cementum / Q000378:metabolism*; D005819:Genetic Markers; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D005819:Genetic Markers; C422903:SOST protein, human