title: Decreased TSPAN1 promotes prostate cancer progression and is a marker for early biochemical recurrence after radical prostatectomy.
abstract: Patients with prostate cancer (PCa) have a variable prognosis. It is challenging to recognize the progressive disease. In this study, we focused on TSPAN1, a new member of the tetraspanin family. Its expression was decreased in progressive PCa and was an independent prognosis factor of biochemical recurrence after radical prostatectomy. In vitro, knockdown and overexpression of TSPAN1 in PCa cell lines showed that TSPAN1 could inhibit cell proliferation and migration. TSPAN1 was positive related to PTEN in both clinical specimen and mouse models. The combination of these two markers could increase their prognosis value especially in low risk patients. In vitro TSPAN1 knockdown resulted in increased Akt phosphorylation and caused evident cell cycle transition from G1 to S phase. Our data suggests that TSPAN1 is a valuable marker to recognize more progressive PCa.
authors.affiliation: Department of Urology, RenJi Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Xu
authors.affiliation: Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan, Ningxia, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Urology, RenJi Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Urology, RenJi Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Jiahua
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Urology, RenJi Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Sha
authors.affiliation: Department of Urology, RenJi Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Shao
authors.affiliation: Division of Oncology, Department of Medicine, University of Missouri School of Medicine, Columbia, MO, USA.
authors.forename: Xunlei
authors.initials: X
authors.lastname: Kang
authors.affiliation: Department of Urology, RenJi Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.|Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.|Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Qin
authors.affiliation: Program of Genes and Development, The University of Texas Graduate School of Biomedical Sciences, Houston, TX, USA.|Department of Hematopathology, Division of Pathology and Laboratory Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: M James
authors.initials: MJ
authors.lastname: You
authors.affiliation: Department of Urology, RenJi Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Yiran
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Urology, RenJi Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Baijun
authors.initials: B
authors.lastname: Dong
authors.affiliation: Department of Urology, RenJi Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xue
mesh_terms: D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015331:Cohort Studies; D018450:Disease Progression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009364:Neoplasm Recurrence, Local; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011379:Prognosis; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000601:surgery; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D059470:Tetraspanins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C508125:TSPAN1 protein, human; D059470:Tetraspanins; D019869:Phosphatidylinositol 3-Kinases; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt
keywords: TSPAN1; biochemical recurrence; prognosis; progression; prostate cancer