title: Enhancement of astaxanthin production from Haematococcus pluvialis mutants by three-stage mutagenesis breeding.
abstract: Haematococcus pluvialis was modified for higher astaxanthin production compatible with the superiorities of high biomass and high activity by three-stage mutagenesis breeding. UV irradiation mutants named UV11-4 made an increase on cell dry weight, but showed a longer growth circle than the wild type. On the basis of UV mutants, ethyl methane sulphonate (EMS) mutants E2-5 cut down the latent phase, brought forward and extended the logarithmic phase. The inhibitor diphenylamine (DPA) was employed to screen high-yield astaxanthin producer by the color change of colonies from green to red on solid medium. Via the contravariant cultivation, proliferation and transformation, the mutant DPA12-2 possessed an 1.7-fold astaxanthin production compared to the wild type, reaching 47.21±3.30mg/g dry cells.
authors.affiliation: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Wang
authors.affiliation: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China. Electronic address: guanbin@ouc.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Guan
authors.affiliation: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Kong
authors.affiliation: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors.forename: Han
authors.initials: H
authors.lastname: Sun
authors.affiliation: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors.forename: Zhaoyan
authors.initials: Z
authors.lastname: Geng
authors.affiliation: School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
authors.forename: Liangfei
authors.initials: L
authors.lastname: Duan
mesh_terms: D018533:Biomass; D001709:Biotechnology; D000460:Chlorophyta / Q000235:genetics / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D016296:Mutagenesis / Q000235:genetics*; D024341:Xanthophylls / Q000032:analysis / Q000378:metabolism
chemical_list: D024341:Xanthophylls; C005948:astaxanthine
keywords: Astaxanthin; Diphenylamine; Haematococcus pluvialis; Three-stage mutagenesis