title: Genomic and proteomic characterization of SE-I, a temperate bacteriophage infecting Erysipelothrix rhusiopathiae.
abstract: A bacteriophage infecting pathogenic Erysipelothrix rhusiopathiae was isolated from a swine farm experiencing an outbreak of acute swine erysipelas; we designated this phage SE-I. SE-I has an icosahedral head, a long tail and a double-stranded DNA genome. The 34,997-bp genome has a GC content of 34 % and contains 43 open reading frames (ORFs) encoding packaging, structural, lysin-holin, and hypothetical proteins. Components of purified SE-I were separated using SDS-PAGE and analyzed using liquid chromatography-mass spectrometry. Nine proteins were identified, encoded by ORF9, ORF15, ORF23, ORF30, ORF31, ORF33, ORF39, ORF40 and ORF 42. A phylogenetic tree constructed based on the sequence of the large terminase subunit revealed that SE-I is closely related to Staphylococcus phages P954 and phi3396. The CHAP-domain-containing protein encoded by ORF25 was expressed in E. coli and which was able to inactivate host bacteria. SE-I was able to infect 7 of 13 E. rhusiopathiae strains, but was unable to infect Salmonella, Streptococcus suis, and Staphylococcus aureus. This is the first report of the isolation, characterization, and genomic and proteomic analysis of a temperate phage infecting E. rhusiopathiae, and it might lead to the development of new anti- E. rhusiopathiae agents.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Yuan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yaning
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Guangcao
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Bai
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. yufengli@njau.edu.cn.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001433:Bacteriolysis; D001435:Bacteriophages / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D001482:Base Composition; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004886:Erysipelas / Q000382:microbiology / Q000662:veterinary; D004888:Erysipelothrix / Q000821:virology*; D004926:Escherichia coli; D000072480:Farms; D023061:Gene Order; D016679:Genome, Viral; D023281:Genomics; D058507:Host Specificity; D013058:Mass Spectrometry; D016366:Open Reading Frames; D010802:Phylogeny; D040901:Proteomics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013211:Staphylococcus aureus; D017011:Streptococcus suis; D013552:Swine; D014764:Viral Proteins / Q000032:analysis*; D014771:Virion / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D004247:DNA