title: Inducible and naturally occurring regulatory T cells enhance lung allergic responses through divergent transcriptional pathways.
abstract: BACKGROUND
  Regulatory T cells attenuate development of asthma in wild-type (WT) mice, with both naturally occurring regulatory T (nTreg) cells and inducible regulatory T (iTreg) cells exhibiting suppressive activity. When transferred into CD8-deficient (CD8-/-) recipients, both cell types enhanced development of allergen-induced airway hyperresponsiveness.
  
  
  OBJECTIVE
  We sought to determine whether the pathways leading to enhancement of lung allergic responses by transferred nTreg and iTreg cells differed.
  
  
  METHODS
  nTreg cells (CD4+CD25+) were isolated from WT mice and iTreg cells were generated from WT CD4+CD25-T cells after activation in the presence of TGF-β and transferred into sensitized CD8-/-recipients before challenge. Development of airway hyperresponsiveness, cytokine levels, and airway inflammation were monitored.
  
  
  RESULTS
  Transfer of nTreg cells enhanced lung allergic responses, as did transfer of iTreg cells. Although anti-IL-13 reduced nTreg cell-mediated enhancement, it was ineffective in iTreg cell-mediated enhancement; conversely, anti-IL-17, but not anti-IL-13, attenuated the enhancement by iTreg cells. Recovered iTreg cells from the lungs of CD8-/-recipients were capable of IL-17 production and expressed high levels of signature genes of the TH17 pathway, RORγt and Il17, whereas reduced expression of the Treg cell key transcription factor forkhead box p3 (Foxp3) was observed. In vitro exogenous IL-6-induced IL-17 production in iTreg cells, and in vivo conversion of transferred iTreg cells was dependent on recipient IL-6.
  
  
  CONCLUSIONS
  iTreg cells, similar to nTreg cells, exhibit functional plasticity and can be converted from suppressor cells to pathogenic effector cells, enhancing lung allergic responses, but these effects were mediated through different pathways.
authors.affiliation: Division of Cell Biology, Department of Pediatrics, National Jewish Health, Denver, Colo.
authors.forename: Anthony
authors.initials: A
authors.lastname: Joetham
authors.affiliation: Division of Cell Biology, Department of Pediatrics, National Jewish Health, Denver, Colo.
authors.forename: Michaela
authors.initials: M
authors.lastname: Schedel
authors.affiliation: Center for Genes, Environment & Health, National Jewish Health, Denver, Colo.
authors.forename: Brian P
authors.initials: BP
authors.lastname: O'Connor
authors.affiliation: Division of Cell Biology, Department of Pediatrics, National Jewish Health, Denver, Colo.
authors.forename: Soohyun
authors.initials: S
authors.lastname: Kim
authors.affiliation: Division of Cell Biology, Department of Pediatrics, National Jewish Health, Denver, Colo.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Takeda
authors.affiliation: Division of Cell Biology, Department of Pediatrics, National Jewish Health, Denver, Colo.
authors.forename: Jordan
authors.initials: J
authors.lastname: Abbott
authors.affiliation: Division of Cell Biology, Department of Pediatrics, National Jewish Health, Denver, Colo. Electronic address: gelfande@njhealth.org.
authors.forename: Erwin W
authors.initials: EW
authors.lastname: Gelfand
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001249:Asthma / Q000276:immunology*; D016535:Bronchial Hyperreactivity / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D004195:Disease Models, Animal; D005434:Flow Cytometry; D006967:Hypersensitivity / Q000276:immunology; D008168:Lung / Q000166:cytology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D060888:Real-Time Polymerase Chain Reaction; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D016207:Cytokines
keywords: IL-13; IL-17; Inducible and naturally occurring regulatory T cells; asthma; enhancement; suppression