title: Analyzing Synthetic Promoters Using Arabidopsis Protoplasts.
abstract: This chapter describes a transient protoplast co-transfection method that can be used to quantitatively study in vivo the activity and function of promoters and promoter elements (reporters), and their induction or repression by transcription factors (effectors), stresses, hormones, or metabolites. A detailed protocol for carrying out transient co-transfection assays with Arabidopsis At7 protoplasts and calculating the promoter activity is provided.
authors.affiliation: Genome Research, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Stracke
authors.affiliation: Genome Research, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Thiedig
authors.affiliation: Genome Research, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Kuhlmann
authors.affiliation: Genome Research, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany. bernd.weisshaar@uni-bielefeld.de.
authors.forename: Bernd
authors.initials: B
authors.lastname: Weisshaar
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics*; D001681:Biological Assay; D018506:Gene Expression Regulation, Plant; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011523:Protoplasts; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D014162:Transfection
chemical_list: D014157:Transcription Factors
keywords: Arabidopsis thaliana; At7 cell culture; Protoplast co-transfection assays; Reporter gene