title: [Expression of miR-181a in Acute Myeloid Leukaemia and Its Effect on Cell Proliferation].
abstract: OBJECTIVE
  To investigate the expression of miR-181a in AML cell lines and explore its effect on cell proliferation.
  
  
  METHODS
  The expression of miR-181a in AML cell lines (NB4,HL-60,K562 and MV-4-11) was detected by quantiative polymerase chain reation(qPCR). Moreover, the cell proliferation and cell cycle were evaluated in several cell lines (HL60, NB4 and K562) by using CCK-8 and flow cytometry after the imitative transfection with miR-181a.
  
  
  RESULTS
  The miR-181a expression was significantly increased in most AML cell lines, including NB4,HL-60 and MV-4-11, but decreased in a few AML cell lines(K562), as compared with that in control(P<0.05). Overexpressed miR-181a in the cell lines significantly enhanced the cell proliferation, as well as the cell ratio of S-to and G2-phase by miR-181a imitative transfection in vitro.
  
  
  CONCLUSIONS
  Overexpression of miR-181a can promote AML cell proliferation. MiR-181a may play an oncogene role in AML, studying the MiR-181a may provide a new method for treatment of AML.
authors.affiliation: Out-patient Department of Pediatrics,Guangzhou Women and Children's Medical Center, Guangzhou 510623, Guangdong Province, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Hematology and Oncology,Guangzhou Women and Children's Medical Center, Guangzhou 510623, Guangdong Province, China.
authors.forename: Mu-Xia
authors.initials: MX
authors.lastname: Yan
authors.affiliation: Department of Hematology and Oncology,Guangzhou Women and Children's Medical Center, Guangzhou 510623, Guangdong Province, China. E-mail: yanwo1990@163.com.
authors.forename: Zi-Yan
authors.initials: ZY
authors.lastname: Luo
mesh_terms: D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D035683:MicroRNAs; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs