title: Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation.
abstract: BACKGROUND
  Our previous study showed that fatty acids extract obtained from CLA-enriched egg yolks (EFA-CLA) suppressed the viability of MCF-7 cancer cell line more effectively than extract from non-enriched egg yolks (EFA). In this study, we analysed the effect of EFA-CLA and EFA on transcriptome profile of MCF-7 cells by applying the whole Human Genome Microarray technology.
  
  
  RESULTS
  We found that EFA-CLA and EFA treated cells differentially regulated genes involved in cancer development and progression. EFA-CLA, compared to EFA, positively increased the mRNA expression of TSC2 and PTEN tumor suppressors as well as decreased the expression of NOTCH1, AGPS, GNA12, STAT3, UCP2, HIGD2A, HIF1A, PPKAR1A oncogenes.
  
  
  CONCLUSIONS
  We show for the first time that EFA-CLA can regulate genes engaged in AKT/mTOR pathway and inhibiting cell cycle progression. The observed results are most likely achieved by the combined effect of both: incorporated CLA isomers and other fatty acids in eggs organically modified through hens' diet. Our results suggest that CLA-enriched eggs could be easily available food products with a potential of a cancer chemopreventive agent.
authors.affiliation: Department of Human Nutrition, Faculty of Food Technology, University of Agriculture, Krakow, Poland.
authors.forename: Aneta A
authors.initials: AA
authors.lastname: Koronowicz
authors.affiliation: Department of Human Nutrition, Faculty of Food Technology, University of Agriculture, Krakow, Poland.
authors.forename: Paula
authors.initials: P
authors.lastname: Banks
authors.affiliation: Department of Human Nutrition, Faculty of Food Technology, University of Agriculture, Krakow, Poland.
authors.forename: Dominik
authors.initials: D
authors.lastname: Domagała
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical Centre for Postgraduate Education, Warsaw, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Master
authors.affiliation: Department of Human Nutrition, Faculty of Food Technology, University of Agriculture, Krakow, Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Leszczyńska
authors.affiliation: Department of Human Nutrition, Faculty of Food Technology, University of Agriculture, Krakow, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Piasna
authors.affiliation: Department of Human Nutrition, Faculty of Food Technology, University of Agriculture, Krakow, Poland.
authors.forename: Mariola
authors.initials: M
authors.lastname: Marynowska
authors.affiliation: Department of Medical Biochemistry, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Laidler
keywords: AKT/mTOR pathway; CLA-enriched egg yolks; Cancer chemoprevention; DNA microarray; MCF-7 cancer cells; SFA/MUFA; Transcriptome