title: Overexpression of the Eggplant (Solanum melongena) NAC Family Transcription Factor SmNAC Suppresses Resistance to Bacterial Wilt.
abstract: Bacterial wilt (BW) is a serious disease that affects eggplant (Solanum melongena) production. Although resistance to this disease has been reported, the underlying mechanism is unknown. In this study, we identified a NAC family transcription factor (SmNAC) from eggplant and characterized its expression, its localization at the tissue and subcellular levels, and its role in BW resistance. To this end, transgenic eggplant lines were generated in which the expression of SmNAC was constitutively up regulated or suppressed using RNAi. The results indicated that overexpression of SmNAC decreases resistance to BW. Moreover, SmNAC overexpression resulted in the reduced accumulation of the plant immune signaling molecule salicylic acid (SA) and reduced expression of ICS1 (a gene that encode isochorismate synthase 1, which is involved in SA biosynthesis). We propose that reduced SA content results in increased bacterial wilt susceptibility in the transgenic lines. Our results provide important new insights into the regulatory mechanisms of bacterial wilt resistance in eggplant.
authors.affiliation: College of Horticulture, South Agricultural University, Guangzhou City, 510642, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Na
authors.affiliation: College of Horticulture, South Agricultural University, Guangzhou City, 510642, P.R. China.
authors.forename: Wu
authors.initials: W
authors.lastname: Shuanghua
authors.affiliation: College of Horticulture, South Agricultural University, Guangzhou City, 510642, P.R. China.
authors.forename: Fu
authors.initials: F
authors.lastname: Jinglong
authors.affiliation: College of Horticulture, South Agricultural University, Guangzhou City, 510642, P.R. China.
authors.forename: Cao
authors.initials: C
authors.lastname: Bihao
authors.affiliation: College of Horticulture, South Agricultural University, Guangzhou City, 510642, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jianjun
authors.affiliation: College of Horticulture, South Agricultural University, Guangzhou City, 510642, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Changming
authors.affiliation: College of Horticulture, South Agricultural University, Guangzhou City, 510642, P.R. China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Jin
mesh_terms: D000595:Amino Acid Sequence; D017343:Genes, Plant; D007150:Immunohistochemistry; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D043368:Ralstonia solanacearum / Q000472:pathogenicity*; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D032323:Solanum melongena / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
chemical_list: D014157:Transcription Factors