title: MicroRNA-506-3p regulates neural stem cell proliferation and differentiation through targeting TCF3.
abstract: Neural stem cells (NSCs) are self-renewing, multipotent and undifferentiated precursors that retain the capacity for differentiation into both glial and neuronal lineages. MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in cell development, differentiation and apoptosis. Recent studies have shown that TCF3 affects neural stem cell proliferation and differentiation. In this study, we predicted that microRNA-506-3p would target TCF3 and demonstrated that miR-506-3p negatively regulates TCF3 expression. The expression level of miR-506-3p was significantly increased during NSC differentiation. In addition, we found that miR-506-3p overexpression increased NSC differentiation and reduced NSC proliferation, indicating an important role of miR-506-3p in NSC. Moreover, the downstream of TCF3, Wnt signaling was significantly decreased with miR-506-3p transfection. These findings suggest that miR-506-3p played an important role in regulating NSC proliferation and differentiation via targeting TCF3, and provide a promising avenue for future in-depth research into the functions of miR-506-3p and TCF3 in nervous system development.
authors.affiliation: Neurological Department of Internal Medicine Ningbo No.2 Hospital, Ningbo 315000, Zhejiang, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Neurological Department of Internal Medicine Ningbo No.2 Hospital, Ningbo 315000, Zhejiang, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Jiaqi
authors.affiliation: Neurological Department of Internal Medicine Ningbo No.2 Hospital, Ningbo 315000, Zhejiang, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhaoying
authors.affiliation: Neurological Department of Internal Medicine Ningbo No.2 Hospital, Ningbo 315000, Zhejiang, China. Electronic address: huiminchennb@163.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Huimin
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D035683:MicroRNAs; C075668:Tcf3 protein, mouse
keywords: MicroRNA-506-3p; Neural stem cell; TCF3