title: Advanced nanostructured medical device combining mesenchymal cells and VEGF nanoparticles for enhanced engineered tissue vascularization.
abstract: OBJECTIVE
  Success of functional vascularized tissue repair depends on vascular support system supply and still remains challenging. Our objective was to develop a nanoactive implant enhancing endothelial cell activity, particularly for bone tissue engineering in the regenerative medicine field.
  
  
  METHODS
  We developed a new strategy of tridimensional implant based on cell-dependent sustained release of VEGF nanoparticles. These nanoparticles were homogeneously distributed within nanoreservoirs onto the porous scaffold, with quicker reorganization of endothelial cells. Moreover, the activity of this active smart implant on cells was also modulated by addition of osteoblastic cells.
  
  
  CONCLUSIONS
  This sophisticated active strategy should potentiate efficiency of current therapeutic implants for bone repair, avoiding the need for bone substitutes.
authors.affiliation: INSERM (French National Institute of Health & Medical Research), "Osteoarticular & Dental Regenerative Nanomedicine" Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg Cedex. FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, F-67000 Strasbourg, France.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Wagner
authors.affiliation: INSERM (French National Institute of Health & Medical Research), "Osteoarticular & Dental Regenerative Nanomedicine" Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg Cedex. FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, F-67000 Strasbourg, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Offner
authors.affiliation: INSERM (French National Institute of Health & Medical Research), "Osteoarticular & Dental Regenerative Nanomedicine" Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg Cedex. FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, F-67000 Strasbourg, France.
authors.forename: Ysia
authors.initials: Y
authors.lastname: Idoux-Gillet
authors.affiliation: School of Pharmacy & Biomolecular Sciences, Liverpool John Moores University, Liverpool, L3 3AF, UK.
authors.forename: Imran
authors.initials: I
authors.lastname: Saleem
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, University College London, 29-39 Brunswick Square, London, WC1N 1AX, UK.
authors.forename: Satyanarayana
authors.initials: S
authors.lastname: Somavarapu
authors.affiliation: INSERM (French National Institute of Health & Medical Research), "Osteoarticular & Dental Regenerative Nanomedicine" Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg Cedex. FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, F-67000 Strasbourg, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Schwinté
authors.affiliation: INSERM (French National Institute of Health & Medical Research), "Osteoarticular & Dental Regenerative Nanomedicine" Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg Cedex. FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, F-67000 Strasbourg, France.
authors.forename: Nadia
authors.initials: N
authors.lastname: Benkirane-Jessel
authors.affiliation: INSERM (French National Institute of Health & Medical Research), "Osteoarticular & Dental Regenerative Nanomedicine" Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg Cedex. FMTS, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 place de l'Hôpital, F-67000 Strasbourg, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Keller
mesh_terms: D000818:Animals; D001846:Bone Development / Q000187:drug effects; D018786:Bone Substitutes / Q000008:administration & dosage / Q000737:chemistry*; D042783:Endothelial Cells / Q000187:drug effects; D006801:Humans; D009389:Neovascularization, Pathologic; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D019736:Prostheses and Implants; D044968:Regenerative Medicine; D023822:Tissue Engineering
chemical_list: D018786:Bone Substitutes
keywords: VEGF nanoparticles; endothelial cells; nanoreservoirs