title: Isolation of 4,5-O-Dicaffeoylquinic Acid as a Pigmentation Inhibitor Occurring in Artemisia capillaris Thunberg and Its Validation In Vivo.
abstract: There is a continual need to develop novel and effective melanogenesis inhibitors for the prevention of hyperpigmentation disorders. The plant Artemisia capillaris Thunberg (Oriental Wormwood) was screened for antipigmentation activity using murine cultured cells (B16-F10 malignant melanocytes). Activity-based fractionation using HPLC and NMR analyses identified the compound 4,5-O-dicaffeoylquinic acid as an active component in this plant. 4,5-O-Dicaffeoylquinic acid significantly reduced melanin synthesis and tyrosinase activity in a dose-dependent manner in the melanocytes. In addition, 4,5-O-dicaffeoylquinic acid treatment reduced the expression of tyrosinase-related protein-1. Significantly, we could validate the antipigmentation activity of this compound in vivo, using a zebrafish model. Moreover, 4,5-O-dicaffeoylquinic acid did not show toxicity in this animal model. Our discovery of 4,5-O-dicaffeoylquinic acid as an inhibitor of pigmentation that is active in vivo shows that this compound can be developed as an active component for formulations to treat pigmentation disorders.
authors.affiliation: New Drug Targets Laboratory, School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
authors.forename: Nadia
authors.initials: N
authors.lastname: Tabassum
authors.affiliation: New Drug Targets Laboratory, School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
authors.forename: Ji-Hyung
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Pharmaceutical Engineering, Dongshin University, 185 Geonjaero, Naju, Jeonnam 520-714, Republic of Korea.
authors.forename: Soon-Ho
authors.initials: SH
authors.lastname: Yim
authors.affiliation: School of Engineering and Applied Sciences, National University of Mongolia, 14200 Ulaanbaatar, Mongolia.
authors.forename: Galzad Javzan
authors.initials: GJ
authors.lastname: Batkhuu
authors.affiliation: New Drug Targets Laboratory, School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
authors.forename: Da-Woon
authors.identifier: 0000-0001-6005-0833
authors.initials: DW
authors.lastname: Jung
authors.affiliation: New Drug Targets Laboratory, School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
authors.forename: Darren R
authors.identifier: 0000-0003-2555-3069
authors.initials: DR
authors.lastname: Williams