title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population.
abstract: Aging is associated with altered immune response, which increases susceptibility to infections. sTREM-1 is involved in the amplification of the inflammatory response to bacterial infection. The present cross-sectional study aims to investigate local sTREM-1 levels in gingival crevicular fluid (GCF) as well as key periodontal pathogen levels in the subgingival plaque in an elderly cohort with periodontal health, gingivitis, and chronic periodontitis (CP).
  
  
  
  Subjects were 51 systemically healthy, elderly individuals (mean age, 68 ± 4.5 years) who had undergone full-mouth periodontal examinations. Subgingival plaque and GCF samples were collected from the healthy sites of participants without periodontal disease (n = 17), the sites with gingival inflammation from patients with gingivitis (n = 19), and the periodontitis sites of patients with CP (n = 15). GCF volumes were measured by an electronic impedance device, and total protein levels were assessed by a flouremetric assay. sTREM-1 levels in GCF were measured by enzyme-linked immunosorbent assay. The subgingival plaque total bacteria, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, and Prevotella intermedia levels were determined by quantitative real-time polymerase chain reaction. Statistical analysis was performed using nonparametric methods.
  
  
  
  GCF volume, total protein concentrations, and sTREM-1 levels in GCF were similar among the groups (p > 0.05). Significantly higher T. forsythia levels were observed in subgingival plaque samples harvested from patients with gingivitis and CP, than in those from healthy participants (p < 0.05). However, the subgingival levels of the other four periodontal pathogens and total bacteria were not statistically different among the groups (p > 0.05).
  
  
  
  Our findings suggest that there are no differences in GCF volume, total protein, and sTREM-1 levels between healthy and periodontally diseased elderly adults. We found only limited differences in the studied subgingival microbial profile. This finding indicates an already deregulated, local inflammatory response in this elderly cohort, on which bacterial biofilm challenge may have a limited further impact.
authors.affiliation: Department of Periodontology, School of Dentistry, Adnan Menderes University, Aydın, Turkey.
authors.forename: Veli Özgen
authors.initials: VÖ
authors.lastname: Öztürk
authors.affiliation: Department of Dental Medicine, Karolinska Institute, Stockholm, Sweden.
authors.forename: Georgios N
authors.initials: GN
authors.lastname: Belibasakis
authors.affiliation: Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey.
authors.forename: Gülnur
authors.initials: G
authors.lastname: Emingil
authors.affiliation: Department of Dental Medicine, Karolinska Institute, Stockholm, Sweden. nagihan.bostanci@ki.se.
authors.forename: Nagihan
authors.initials: N
authors.lastname: Bostanci
mesh_terms: D000368:Aged; D000375:Aging; D003430:Cross-Sectional Studies; D004269:DNA, Bacterial / Q000302:isolation & purification / Q000378:metabolism; D003773:Dental Plaque / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016967:Fusobacterium nucleatum / Q000235:genetics / Q000302:isolation & purification; D005883:Gingival Crevicular Fluid / Q000378:metabolism; D005891:Gingivitis / Q000382:microbiology / Q000473:pathology; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D010518:Periodontitis / Q000382:microbiology / Q000473:pathology; D010519:Periodontium / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D011971:Receptors, Immunologic / Q000032:analysis*; D000070436:Tannerella forsythia / Q000235:genetics / Q000302:isolation & purification; D000073871:Triggering Receptor Expressed on Myeloid Cells-1
chemical_list: D004269:DNA, Bacterial; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1
keywords: Aging; Elderly; Gingival crevicular fluid; Subgingival plaque; sTREM-1