title: [Effect of rhG-CSF Mobilization on S1P5 Expression in T Lymphocyte Subsets of Allo-HSCT Donors].
abstract: OBJECTIVE
  To explore the effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) mobilization on S1P5 expression in T lymphocyte subsets of allo-HSCT donors.
  
  
  METHODS
  The peripheral blood was collected from 10 allo-hematopoietic stem cell transplantation (allo-HSCT) donors before and after mobilization with rhG-CSF for 4 days. The flow cytometry was used to detect S1P5 expression in T lymphocyte subsets.
  
  
  RESULTS
  There was no S1P5 expression on the surface of T-lymphocytes both before and after rhG-CSF mobilization. After fixation with permeabilization agent, S1P5 expression could be detected in lymphocytes after rhG-CSF mobilization, which indicates S1P5 may be located in cells. Compared with level before rhG-CSF mobilization, S1P5 expression was significantly increased in T lymphocyte subsets after rhG-CSF mobilization, CD3(+)T cells (57.92±2.32)% vs (7.94±1.47)%(P<0.05）, CD4(+)T cells (72.58±1.73)% vs （5.48±0.82）%(P<0.05）, CD8(+)T cells（51.79±3.57）% vs （6.46±1.01）%（P<0.05）,CD3-/CD56(+)NK cells（40.00±1.47）% vs（4.97±0.74）%（P<0.05）. The up-regulated level of S1P5 expression in CD4(+)T cells was most high(P<0.05).
  
  
  CONCLUSIONS
  S1P5 expression significantly increases in T lymphocyte subsets after rhG-CSF mobilization, and the up-regulated level of S1P5 expression in CD4(+)T cells is the most high.
authors.affiliation: Department of Hematology, Hainan Branch of Chinese PLA General Hospital, Sanya 572000, Hainan Province, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Hematology, Hainan Branch of Chinese PLA General Hospital, Sanya 572000, Hainan Province, China.
authors.forename: Li-Xun
authors.initials: LX
authors.lastname: Guan
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Zhen-Yang
authors.initials: ZY
authors.lastname: Gu
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Zhao
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Fei-Yan
authors.initials: FY
authors.lastname: Wang
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Hua-Ping
authors.initials: HP
authors.lastname: Wei
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Hematology, Hainan Branch of Chinese PLA General Hospital, Sanya 572000, Hainan Province, China.
authors.forename: Hong-Hua
authors.initials: HH
authors.lastname: Li
authors.affiliation: Department of Hematology, Hainan Branch of Chinese PLA General Hospital, Sanya 572000, Hainan Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Bo
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China. E-mail：gaochunji@medmail.com.cn.
authors.forename: Chun-Ji
authors.initials: CJ
authors.lastname: Gao
mesh_terms: D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D049349:Receptors, Lysosphingolipid; D011994:Recombinant Proteins; D016176:T-Lymphocyte Subsets; D014184:Transplantation, Homologous
chemical_list: D049349:Receptors, Lysosphingolipid; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor