title: Absence of co-localization between pathovar-associated virulence factors and extended-spectrum β-lactamase (blaCTX-M) genes on a single plasmid.
abstract: Extended-spectrum β-lactamases (ESBLs) were reported in virulent food-borne Escherichia coli clones, and numerous genes encoding ESBLs and virulence factors (VFs) are plasmid-mediated. We investigated the plasmidic co-localization of ESBL genes and pathovar-associated VF genes isolated in 18 E. coli isolates from faecal samples of diseased cattle. From the rare ESBL-producing E. coli among the various pathovars, no plasmid co-localization was found between VF and blaCTX-M genes on a single plasmid. However, a link between replicon types and VFs was highlighted: EspP was associated with IncFIB and ToxB with IncB/O. Associations of IncF alleles to VF or CTX-M-types were also identified: CS31A was linked to the allele FIB38 and CTX-M-14 to IncFII2. Also, as illustrated here, IncFII and IncFIB were carried by two different plasmids in a single cell.
authors.affiliation: Anses, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactériennes, Lyon, France. Electronic address: charlotte.valat@anses.fr.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Valat
authors.affiliation: Anses, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Forest
authors.affiliation: Anses, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
authors.forename: Méganne
authors.initials: M
authors.lastname: Billet
authors.affiliation: Anses, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
authors.forename: Charlène
authors.initials: C
authors.lastname: Polizzi
authors.affiliation: Anses, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Saras
authors.affiliation: Anses, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Madec
authors.affiliation: Anses, Laboratoire de Lyon, Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
authors.forename: Marisa
authors.initials: M
authors.lastname: Haenni
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D010957:Plasmids / Q000235:genetics*; D021381:Protein Transport; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors; D001618:beta-Lactamases; C000602253:beta-lactamase CTX-M, E coli
keywords: CTX-M and virulence; Escherichia coli; Plasmid