title: Cytotoxic Responses and Apoptosis in Rat Kidney Epithelial Cells Exposed to Lead.
abstract: The toxic effects of lead on normal rat kidney epithelial cells (NRK cells) may occur via various pathways. However, the role of intrinsic mitochondrial pathway in Lead-induced apoptosis in NRK cells has not been investigated. The purpose of our study was to investigate cytotoxic responses and cell apoptosis mediated by lead in NRK cells. NRK cells were treated with different concentrations of Lead acetate for 12 h to determine the cytotoxicity of lead. Mitochondrial transmembrane potential was also analyzed using a fluorescence spectrophotometer. Moreover, the activities of caspase-3 and caspase-9 were detected in the presence of lead. Finally, the lead-induced cell apoptosis was evaluated by flow cytometry in the present of caspase inhibitors Z-VAD-FMK and Ac-LEHD-FMK, respectively. The results would contribute to clarify the role of Lead in proliferation and apoptosis of NRK cells, and help to understand the underlying mechanism responsible for lead-induced cell apoptosis.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Xiu Yuan
authors.initials: XY
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, Hunan, China.
authors.forename: Li Yun
authors.initials: LY
authors.lastname: Yuan
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Yong Tao
authors.initials: YT
authors.lastname: Li
authors.affiliation: Kansas State University, Manhattan 66506, the United States of America.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan, China; College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, Hunan, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, Hunan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yuan
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, Hunan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, Hunan, China.
authors.forename: Cheng Zhi
authors.initials: CZ
authors.lastname: Guo
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D007668:Kidney / Q000166:cytology*; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats
chemical_list: D009942:Organometallic Compounds; C008261:lead acetate