title: Cytotoxicity and cytokine expression induced by silorane and methacrylate-based composite resins.
abstract: OBJECTIVE
  The aim of this study was to evaluate cytotoxicity and cytokine production induced by light-cured or non-light-cured methacrylate-based and silorane composite resins in RAW 264.7 macrophages.
  
  
  METHODS
  Cells were stimulated with the extracts from light-cured or non-light-cured composite resins. After incubation for 24 h, cytotoxicity was assessed with the lactate dehydrogenase (LDH) and methyl thiazolyl tetrazolium (MTT) assays, and total protein was quantified using the Lowry method. TNF-α detection was examined with an enzyme-linked immunosorbent assay (ELISA) conducted with cell supernatants after cell stimulation for 6, 12, and 24 h. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey's post hoc test (α=0.05).
  
  
  RESULTS
  KaloreTM and FiltekTM Silorane were cytotoxic with or without light curing (p<0.05) after 24 h of incubation. KaloreTM stimulated the early production of TNF-α in comparison with control (p<0.05), whereas FiltekTM Silorane did not affect TNF-α levels after 6 and 12 h (p>0.05). However, after 24 h FiltekTM Silorane inhibited the production of TNF-α (p<0.05).
  
  
  CONCLUSIONS
  KaloreTM and FiltekTM Silorane were cytotoxic regardless of light curing. The extract obtained from KaloreTM after 15 days of incubation stimulated the production of TNF-α, unlike that obtained from FiltekTM Silorane.
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Clínica Infantil, Ribeirão Preto, SP, Brasil.
authors.forename: Daniele Lucca
authors.initials: DL
authors.lastname: Longo
authors.affiliation: - Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Departamento de Análises Clínicas, Ribeirão Preto, SP, Brasil.
authors.forename: Francisco Wanderley Garcia
authors.initials: FW
authors.lastname: Paula-Silva
authors.affiliation: - Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Departamento de Análises Clínicas, Ribeirão Preto, SP, Brasil.
authors.forename: Lucia Helena
authors.initials: LH
authors.lastname: Faccioli
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Clínica Infantil, Ribeirão Preto, SP, Brasil.|- Universitat de Barcelona, Facultat d'Odontologia, Department d'Odontopediatria, Barcelona, España.
authors.forename: Patrícia Maria
authors.initials: PM
authors.lastname: Gatón-Hernández
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Clínica Infantil, Ribeirão Preto, SP, Brasil.
authors.forename: Alexandra Mussolino de
authors.initials: AM
authors.lastname: Queiroz
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Clínica Infantil, Ribeirão Preto, SP, Brasil.
authors.forename: Léa Assed Bezerra da
authors.initials: LA
authors.lastname: Silva
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003188:Composite Resins / Q000528:radiation effects / Q000633:toxicity*; D055117:Curing Lights, Dental; D004797:Enzyme-Linked Immunosorbent Assay; D007770:L-Lactate Dehydrogenase; D008422:Materials Testing; D008689:Methacrylates / Q000528:radiation effects / Q000633:toxicity*; D051379:Mice; D012016:Reference Values; D015203:Reproducibility of Results; D062047:Silorane Resins / Q000528:radiation effects / Q000633:toxicity*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D003188:Composite Resins; C000612380:Kalore GC; D008689:Methacrylates; D062047:Silorane Resins; D014409:Tumor Necrosis Factor-alpha; C555513:silorane composite resin; D007770:L-Lactate Dehydrogenase