title: Integrated multi-biomarker responses in two dreissenid species following metal and thermal cross-stress.
abstract: With current global changes, the combination of several stressors such as temperature and contaminants may impact species distribution and ecosystem functioning. In this study, we evaluated the combined impact of two metals (Ni and Cr) with a thermal stress (from 12 to 17 °C) on biomarker responses in two bivalves, Dreissena rostriformis bugensis and Dreissena polymorpha. Biomarkers are informative tools to evaluate exposure and effects of stressors on organisms. The set of 14 biomarkers measured here was representative of both physiologic (filtration activity) and cellular antioxidant and detoxification mechanisms. Our aim was to study the response pattern of both species, and its meaning in terms of invasive potential. The implications for the use of these mussels in environmental monitoring are also discussed. Results evidenced that the two species do not respond to multiple stressors in the same way. Indeed, the effects of contamination on biomarker responses were more marked for D. polymorpha, especially under nickel exposure. While we cannot conclude as to the effect of temperature, invasiveness could be influenced by species sensitivity to contaminants. The physiological and cellular differences between D. polymorpha and D. r. bugensis might also be of concern for environmental risk assessment. The two species present differential bioaccumulation patterns, filtration activity and cellular biomarker responses. If D. polymorpha populations decline, their substitution by D. r. bugensis for biomonitoring or laboratory studies will not be possible without a deeper understanding of biomarker responses of the new invasive.
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France. Electronic address: marine.potet@univ-lorraine.fr.
authors.forename: Marine
authors.initials: M
authors.lastname: Potet
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France. Electronic address: simon.devin@univ-lorraine.fr.
authors.forename: Simon
authors.initials: S
authors.lastname: Devin
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France. Electronic address: sandrine.devin@univ-lorraine.fr.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Pain-Devin
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France. Electronic address: philippe.rousselle@univ-lorraine.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Rousselle
authors.affiliation: Université de Lorraine, CNRS UMR 7360, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France. Electronic address: laure.giamberini@univ-lorraine.fr.
authors.forename: Laure
authors.initials: L
authors.lastname: Giambérini
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D049875:Dreissena / Q000187:drug effects* / Q000378:metabolism; D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000633:toxicity*; D009532:Nickel / Q000633:toxicity*; D013312:Stress, Physiological / Q000187:drug effects*; D013696:Temperature
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D009532:Nickel
keywords: Biomarker; Dreissena polymorpha; Dreissena rostriformis bugensis; Multi-stress; Temperature