title: Modified Rapid AChE Method (MRAM) for Hirschsprung Disease Diagnosis: A Journey from Meier-Ruge Until Now.
abstract: Hirschsprung disease (HSCR) can be diagnosed using a variety of histological and immunohistochemical methods and stains. Because of the nature of the condition and the need for a rapid diagnostic confirmation, those methods with high accuracy and fast turnaround times are preferred. The authors of this paper have used rapid acetylcholinesterase (AChE) immunohistochemistry in conjunction with standard H&E in order to optimize diagnostic accuracy, and present a modified rapid AChE method (MRAM) that has been successfully utilized for over 20 years. The authors also present a list of over 30 different methods and stains that have been proposed for Hirschsprung disease diagnosis.
authors.affiliation: a Department of Pathology , Emory University School of Medicine , Atlanta , Georgia , USA.
authors.forename: Mina M
authors.initials: MM
authors.lastname: Naguib
authors.affiliation: b Department of Pathology , Todelo Children's Hospital , Toledo , Ohio , USA.
authors.forename: Haynes
authors.initials: H
authors.lastname: Robinson
authors.affiliation: a Department of Pathology , Emory University School of Medicine , Atlanta , Georgia , USA.
authors.forename: Carla
authors.initials: C
authors.lastname: Shoffeitt
authors.affiliation: b Department of Pathology , Todelo Children's Hospital , Toledo , Ohio , USA.
authors.forename: Helena
authors.initials: H
authors.lastname: Howe
authors.affiliation: a Department of Pathology , Emory University School of Medicine , Atlanta , Georgia , USA.
authors.forename: Diana
authors.initials: D
authors.lastname: Metry
authors.affiliation: a Department of Pathology , Emory University School of Medicine , Atlanta , Georgia , USA.
authors.forename: Bahig M
authors.initials: BM
authors.lastname: Shehata
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D001707:Biopsy, Needle / Q000379:methods; D002648:Child; D002675:Child, Preschool; D003581:Cytodiagnosis / Q000379:methods; D005260:Female; D006627:Hirschsprung Disease / Q000175:diagnosis* / Q000201:enzymology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007223:Infant; D007231:Infant, Newborn; D008297:Male
chemical_list: D015415:Biomarkers; D000110:Acetylcholinesterase
keywords: Hirschsprung disease; acetylcholinesterase; immunohistochemistry; modified rapid AChE method