title: H2A deubiquitinases UBP12/13 are part of the Arabidopsis polycomb group protein system.
abstract: Polycomb group (PcG) proteins form an epigenetic memory system in plants and animals, but interacting proteins are poorly known in plants. Here, we have identified Arabidopsis UBIQUITIN SPECIFIC PROTEASES (USP; UBP in plant and yeasts) 12 and 13 as partners of the plant-specific PcG protein LIKE HETEROCHROMATIN PROTEIN 1 (LHP1). UBP12 binds to chromatin of PcG target genes and is required for histone H3 lysine 27 trimethylation and repression of a subset of PcG target genes. Plants lacking UBP12 and UBP13 developed autonomous endosperm in the absence of fertilization. We have identified UBP12 and UBP13 as new proteins in the plant PcG regulatory network. UBP12 and UBP13 belong to an ancient gene family and represent plant homologues of metazoan USP7. We have found that Drosophila USP7 shares a function in heterochromatic gene repression with UBP12/13 and their homologue UBP26. In summary, we demonstrate that USP7-like proteins are essential for gene silencing in diverse genomic contexts.
authors.affiliation: Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Centre for Plant Biology, SE-75007 Uppsala, Sweden.|Department of Biology and Zurich-Basel Plant Science Centre, ETH Zurich, CH-8092, Zurich, Switzerland.
authors.forename: Maria
authors.initials: M
authors.lastname: Derkacheva
authors.affiliation: Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Centre for Plant Biology, SE-75007 Uppsala, Sweden.
authors.forename: Shujing
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Centre for Plant Biology, SE-75007 Uppsala, Sweden.
authors.forename: Duarte D
authors.identifier: 0000-0001-7990-3592
authors.initials: DD
authors.lastname: Figueiredo
authors.affiliation: Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Centre for Plant Biology, SE-75007 Uppsala, Sweden.
authors.forename: Matthew
authors.initials: M
authors.lastname: Gentry
authors.affiliation: Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Centre for Plant Biology, SE-75007 Uppsala, Sweden.
authors.forename: Iva
authors.identifier: 0000-0002-3815-9223
authors.initials: I
authors.lastname: Mozgova
authors.affiliation: Functional Genomics Centre Zurich, University of Zurich/ETH Zürich, CH-8057 Zurich, Switzerland.
authors.forename: Paolo
authors.initials: P
authors.lastname: Nanni
authors.affiliation: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden.
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.affiliation: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden.
authors.forename: Mattias
authors.initials: M
authors.lastname: Mannervik
authors.affiliation: Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Centre for Plant Biology, SE-75007 Uppsala, Sweden.
authors.forename: Claudia
authors.initials: C
authors.lastname: Köhler
authors.affiliation: Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Centre for Plant Biology, SE-75007 Uppsala, Sweden.
authors.forename: Lars
authors.identifier: 0000-0002-6645-1862
authors.initials: L
authors.lastname: Hennig
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D063146:Polycomb-Group Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D063146:Polycomb-Group Proteins; D010450:Endopeptidases; C561399:UBP12 protein, Arabidopsis; C561400:UBP13 protein, Arabidopsis