title: Molecular characterization of the species Salvinia (Salviniaceae) from the upper Paraná River floodplain.
abstract: The pteridophytes Salvinia minima, S. herzogii, and S. auriculata are among the most abundant aquatic macrophytes in the upper Paraná River floodplain. Since some species have highly similar morphological features, it is very difficult to identify members of this genus to the species level. An indication of this difficulty is a set of poorly differentiated taxa comprising S. auriculata and S. herzogii known as the 'S. auriculata complex', which is found in the Paraná River together with other Salvinia species such as S. biloba and S. molesta. Some authors have reported the existence of inter-species hybrids. Despite the complex Salvinia taxonomy, few genetic studies have been performed on purported species within the genus to resolve this complexity. The present study was conducted to determine useful molecular sequences for the discrimination of Salvinia species of the upper Paraná River floodplain. Molecular data were compared with data of other species of the genus to clarify phylogenetic relationships, employing the nucleotide sequence trnL-trnF from the chloroplast DNA. The results revealed that Salvinia populations in the upper Paraná River floodplain belong to different species and indicated that species of the S. auriculata complex may be distinguished from one another after the division of the S. minima group, corroborating results by other researchers. Although the taxonomic position of S. oblongifolia was clarified, as high closeness between S. oblongifolia and the S. auriculata complex was reported, Salvinia kinship is still not thoroughly established and further investigations in morphology and molecular diversity are required.
authors.affiliation: Programa de Pós-Graduação em Biologia Comparada, Universidade Estadual de Maringá, PR, Brasil.
authors.forename: S A
authors.initials: SA
authors.lastname: Machado
authors.affiliation: Núcleo de Pesquisa em Limnologia, Ictiologia e Aquicultura, Universidade Estadual de Maringá, Maringá, PR, Brasil alessoli@hotmail.com.|Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, PR, Brasil alessoli@hotmail.com.
authors.forename: A V
authors.initials: AV
authors.lastname: Oliveira
authors.affiliation: Programa de Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais, Universidade Estadual de Maringá, PR, Brasil.|Núcleo de Pesquisa em Limnologia, Ictiologia e Aquicultura, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: T M C
authors.initials: TM
authors.lastname: Fabrin
authors.affiliation: Núcleo de Pesquisa em Limnologia, Ictiologia e Aquicultura, Universidade Estadual de Maringá, Maringá, PR, Brasil.|Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: S M A P
authors.initials: SM
authors.lastname: Prioli
authors.affiliation: Núcleo de Pesquisa em Limnologia, Ictiologia e Aquicultura, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: A J
authors.initials: AJ
authors.lastname: Prioli
mesh_terms: D044822:Biodiversity; D018742:DNA, Chloroplast / Q000235:genetics; D017343:Genes, Plant; D058889:Molecular Typing; D010802:Phylogeny; D045483:Rivers; D017422:Sequence Analysis, DNA; D064028:Tracheophyta / Q000235:genetics*
chemical_list: D018742:DNA, Chloroplast