title: The Use of a Dexamethasone-inducible System to SynchronizeXa21Expression to Study Rice Immunity.
abstract: Inducible gene expression systems offer researchers the opportunity to synchronize target gene expression at particular developmental stages and in particular tissues. The glucocorticoid receptor (GR), a vertebrate steroid receptor, has been well adopted for this purpose in plants. To generate steroid-inducible plants, a construct of GAL4-binding domain-VP16 activation domain-GR fusion (GVG) with the target gene under the control of upstream activation sequence (UAS) has been developed and extensively used in plant research. Immune receptors perceive conserved molecular patterns secreted by pathogens and initiate robust immune responses. The rice immune receptor,XA21, recognizes a molecular pattern highly conserved in all sequenced genomes ofXanthomonas, and confers robust resistance toX. oryzaepv.oryzae(Xoo). However, identifying genes downstream of XA21 has been hindered because of the restrained lesion and thus limited defense response region in the plants expressingXa21. Inducible expression allows for a synchronized immune response across a large amount of rice tissue, well suited for studying XA21-mediated immunity by genome-wide approaches such as transcriptomics and proteomics. In this protocol, we describe the use of this GVG system to synchronizeXa21expression.
authors.affiliation: Department of Plant Pathology, University of California Davis, Davis, USA.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Caddell
authors.affiliation: Department of Plant Pathology, University of California Davis, Davis, USA.
authors.forename: Tong
authors.initials: T
authors.lastname: Wei
authors.affiliation: Department of Bioresources Engineering, Sejong University, Seoul, Republic of Korea.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Park
authors.affiliation: Department of Plant Pathology, University of California Davis, Davis, USA.
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Ronald