title: A Novel Process for the Synthesis of Highly Pure n-3 Polyunsaturated Fatty Acid (PUFA)-Enriched Triglycerides by Combined Transesterification and Ethanolysis.
abstract: In this study, a novel two-step enzymatic reaction was developed for the synthesis of highly pure triacylglycerols (TAGs) with a high content of n-3 polyunsaturated fatty acids (PUFAs). Glyceride mixtures were primarily synthesized by Novozym 435-catalyzed transesterification of glycerol and DHA/EPA-rich ethyl esters (EEs), followed by removal of partial glycerides, for the first time, by immobilized mono- and diacylglycerol lipase SMG1-F278N-catalyzed ethanolysis. TAG yield as high as 98.66% was achieved under the optimized conditions, and highly pure (98.75%) n-3 PUFA-enriched TAGs with 88.44% of n-3 PUFA was obtained after molecular distillation at lower temperature (140 °C). In addition, the EEs produced during ethanolysis had a FA composition similar to that of the original EEs, making them feasible for cyclic utilization. This was the first study reporting removal of partial glycerides by ethanolysis. Through ethanolysis, a higher purity product could be easily obtained at a relatively low temperature compared with the conventional high-temperature molecular distillation.
authors.affiliation: School of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
authors.forename: Daoming
authors.initials: D
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology , Guangzhou 510640, China.
authors.forename: Weifei
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Food Science, Southwest University , Chongqing 400715, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Qin
authors.affiliation: School of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology , Guangzhou 510006, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: School of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D055162:Biocatalysis; D002384:Catalysis; D004800:Enzymes, Immobilized; D004951:Esterification; D004986:Ether / Q000737:chemistry*; D015525:Fatty Acids, Omega-3 / Q000138:chemical synthesis*; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D014280:Triglycerides / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D015525:Fatty Acids, Omega-3; D005656:Fungal Proteins; D014280:Triglycerides; D004986:Ether; C093667:Novozyme 435; D008049:Lipase
keywords: 13C NMR; ethanolysis; mono- and diacylglycerol lipase; n-3 polyunsaturated fatty acids; triacylglycerols