title: [Sclerostin expression in periodontal ligaments during movement of orthodontic teeth in rats].
abstract: OBJECTIVE
  This study aims to observe the expression of Sclerostin during movement of orthodontic teeth and determine the effect of this protein on remodeling of periodontal tissues.
  
  
  METHODS
  Twenty-four Wistar rats were chosen. Orthodontic forces were applied between the bilateral incisor and first molar to achieve mesial movement. Rats in each group were executed at different time points (0, 1, 3, 5, 7, 14 d). Morphology of periodontal tissue was observed by hematoxylin-eosin (HE) staining. The number of osteoclasts were observed by tartrate-resistant acid phosphatase (TRAP) staining. Sclerostin expression were observed by immunohistochemical staining.
  
  
  RESULTS
  HE staining revealed that the resorption of alveolar bone intensified with prolonged movement. Results of immunohistochemical and TRAP staining revealed that Sclerostin expression and number of osteoclasts were related to duration of movement of orthodontic tooth. After staining for 5 days, the number of osteoclasts and Sclerostin expression reached their peak and then began to decline. The numbers of osteoclasts and the expression level of Sclerostin were higher at the compressive side than those at the tensive side.
  
  
  CONCLUSIONS
  Sclerostin affected orthodontic tooth movement by inhibiting the Wnt signaling pathway and by indirectly or directly controlling bone morphogenetic protein.
authors.forename: Chen
authors.initials: C
authors.lastname: Yiwen
authors.forename: Gao
authors.initials: G
authors.lastname: Shang
authors.forename: Xu
authors.initials: X
authors.lastname: Tongtong
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jiahui
authors.forename: Li
authors.initials: L
authors.lastname: Jincheng
authors.forename: Zhang
authors.initials: Z
authors.lastname: Huiyan
authors.forename: Lu
authors.initials: L
authors.lastname: Jinjin
authors.forename: Hu
authors.initials: H
authors.lastname: Min
authors.forename: Liu
authors.initials: L
authors.lastname: Zhihui
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D005819:Genetic Markers; D007180:Incisor; D008963:Molar; D009068:Movement; D010010:Osteoclasts; D010513:Periodontal Ligament / Q000378:metabolism*; D010519:Periodontium; D051381:Rats; D017208:Rats, Wistar; D014087:Tooth Movement Techniques
chemical_list: D019485:Bone Morphogenetic Proteins; D005819:Genetic Markers; C486031:Sost protein, rat