title: Anticoagulant activity of a natural protein purified from Hypomesus olidus.
abstract: A novel anticoagulant protein (E-II-1) was separated and purified from Hypomesus olidus, a unique freshwater fish in northern China. E-II-1 had a molecular mass of approximately 40 kDa with no subunits. The high content of hydrophobic amino acids and negatively charged amino acids in E-II-1 demonstrated that the amino acid compositions might contribute to the anticoagulant activity. E-II-1 contained α-helices 16.75%, β-sheets 42.67%, β-turn 25.58% and random coil 15.00%. In vitro blood coagulation time assay, E-II-1 significantly prolonged the activated partial thrombin time in a dose-dependent manner. Results indicated that E-II-1 acted as anticoagulants through the endogenous pathway with an inhibition of FXa. The specific activity of E-II-1 was 103.50 U/mg at a concentration of 1.00 mg/mL. Therefore, E-II-1 might be one of the promising anticoagulants originated from natural food sources with more safety and less side effects.
authors.affiliation: a Department of Food Science and Engineering , Jilin Agricultural University , Changchun , P.R. China.
authors.forename: Mengxing
authors.initials: M
authors.lastname: Gou
authors.affiliation: a Department of Food Science and Engineering , Jilin Agricultural University , Changchun , P.R. China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Wang
authors.affiliation: a Department of Food Science and Engineering , Jilin Agricultural University , Changchun , P.R. China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Liu
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D002681:China; D004305:Dose-Response Relationship, Drug; D015951:Factor Xa / Q000187:drug effects; D029941:Fish Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005399:Fishes / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008970:Molecular Weight; D010314:Partial Thromboplastin Time; D011487:Protein Conformation; D011517:Prothrombin Time; D013918:Thrombin Time
chemical_list: D000596:Amino Acids; D000925:Anticoagulants; D029941:Fish Proteins; D015951:Factor Xa
keywords: Hypomesus olidus; activated partial thrombin time; anticoagulant protein; chromatographic column; purification