title: Influence of Aeromonas hydrophila and Pseudomonas fluorescens on motility, viability and morphometry of cryostored silver barb (Barbodes gonionotus) sperm.
abstract: This objective of the study was to evaluate the effects of A. hydrophila subsp. hydrophila and P. fluorescens on sperm motility, sperm viability and sperm morphometry of cryopreserved silver barb (Barbodes gonionotus) semen and survival of tested bacteria after cryostorage. Semen was diluted in a calcium-free Hank's balanced salt solution (Ca-F HBSS) supplemented with or without 0.25% penicillin-streptomycin (PS) after which A. hydrophila subsp. hydrophila or P. fluorescens was immediately added into extended semen prior to freezing. Extended semen and cryostored semen kept for 20 min, 24 h, 7 d, 14 d and 28 d were assessed for sperm motility, sperm viability, sperm morphometry, survival of challenged bacteria and the relationship between bacteria and sperm. Bacterial-exposed semen with or without 0.25% PS supplementation showed a significant reduction (P < 0.05) in sperm motility and viability during a cryostorage of 28 d, compared to semen without bacterial supplementation (control groups). Addition of A. hydrophila subsp. hydrophila and P. fluorescens resulted in a significant (P < 0.05) alteration of sperm morphometry of cryopreserved semen, especially flagellum width. The two pathogens were detected at a level of 10(5) CFU ml(-1) in cryostored semen with or without antibiotic supplementation. There were significant correlations among bacterial number, percentage of sperm motility and viability and flagellum width. In conclusion, the presence of A. hydrophila subsp. hydrophila and P. fluorescens had a deleterious effect on cryopreserved silver barb sperm based on a reduction in sperm motility and viability and alteration of sperm morphometry, especially flagellum width.
authors.affiliation: Biological Science Program, Faculty of Science, Burapha University, Chon Buri, 20131, Thailand. Electronic address: boonthait@gmail.com.
authors.forename: Traimat
authors.initials: T
authors.lastname: Boonthai
authors.affiliation: Environmental Science Program, Faculty of Science, Burapha University, Chon Buri, 20131, Thailand. Electronic address: s.coccus@hotmail.com.
authors.forename: Weerasith
authors.initials: W
authors.lastname: Khaopong
authors.affiliation: Environmental Science Program, Faculty of Science, Burapha University, Chon Buri, 20131, Thailand. Electronic address: Jumlong_Sangsong@hotmail.com.
authors.forename: Jumlong
authors.initials: J
authors.lastname: Sangsong
authors.affiliation: Department of Aquatic Science, Faculty of Science, Burapha University, Chon Buri, 20131, Thailand. Electronic address: verapong@buu.ac.th.
authors.forename: Verapong
authors.initials: V
authors.lastname: Vuthiphandchai
authors.affiliation: Department of Microbiology and Environmental Science Program, Faculty of Science, Burapha University, Chon Buri, 20131, Thailand. Electronic address: subunti@buu.ac.th.
authors.forename: Subuntith
authors.initials: S
authors.lastname: Nimrat
mesh_terms: D016980:Aeromonas hydrophila / Q000472:pathogenicity*; D000818:Animals; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary; D003530:Cyprinidae; D005615:Freezing; D008297:Male; D011551:Pseudomonas fluorescens / Q000472:pathogenicity*; D012661:Semen / Q000382:microbiology* / Q000502:physiology; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
keywords: Aeromonas hydrophila; Barbodes gonionotus; Cryopreservation; Morphometry; Pseudomonas fluorescens; Sperm quality