title: Economic analysis of pilot-scale production of B-phycoerythrin.
abstract: β-Phycoerythrin is a color protein with several applications, from food coloring to molecular labeling. Depending on the application, different purity is required, affecting production cost and price. Different production and purification strategies for B-phycoerythrin have been developed, the most studied are based on the production using Porphyridium cruentum and purified using chromatographic techniques or aqueous two-phase systems. The use of the latter can result in a less expensive and intensive recovery of the protein, but there is lack of a proper economic analysis to study the effect of using aqueous two-phase systems in a scaled-up process. This study analyzed the production of B-Phycoerythrin using real data obtained during the scale-up of a bioprocess using specialized software (BioSolve, Biopharm Services, UK). First, a sensitivity analysis was performed to identify critical parameters for the production cost, then a Monte Carlo analysis to emulate real processes by adding uncertainty to the identified parameters. Next, the bioprocess was analyzed to determine its financial attractiveness and possible optimization strategies were tested and discussed. Results show that aqueous two-phase systems retain their advantages of low cost and intensive recovery (54.56%); the costs of production per gram calculated (before titer optimization: US$15,709 and after optimization: US$2,374) allowed to obtain profit (in the range of US$millions in a 10-year period) for a potential company taking this production method by comparing the production cost against commercial prices. The bioprocess analyzed is a promising and profitable method for the generation of a highly purified B-phycoerythrin. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1472-1479, 2016.
authors.affiliation: Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL, 64849, México.
authors.forename: Mario A
authors.identifier: 0000-0002-0846-2184
authors.initials: MA
authors.lastname: Torres-Acosta
authors.affiliation: Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL, 64849, México.
authors.forename: Federico
authors.initials: F
authors.lastname: Ruiz-Ruiz
authors.affiliation: Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL, 64849, México.
authors.forename: José M
authors.initials: JM
authors.lastname: Aguilar-Yáñez
authors.affiliation: Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL, 64849, México.
authors.forename: Jorge
authors.initials: J
authors.lastname: Benavides
authors.affiliation: Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL, 64849, México.
authors.forename: Marco
authors.initials: M
authors.lastname: Rito-Palomares
mesh_terms: D002623:Chemistry Techniques, Analytical; D002845:Chromatography; D003365:Costs and Cost Analysis; D009010:Monte Carlo Method; D010799:Phycoerythrin / Q000096:biosynthesis* / Q000737:chemistry / Q000191:economics*; D044665:Porphyridium / Q000737:chemistry / Q000378:metabolism*; D012984:Software / Q000191:economics*; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D010799:Phycoerythrin
keywords: Aqueous two-phase systems; B-Phycoerythrin; Economic analysis; Monte Carlo simulation; uncertainty