title: Development and validation of an LC-MS/MS method for the determination of hinokiflavone in rat plasma and its application to a pharmacokinetic study.
abstract: Hinokiflavone has drawn a lot of attention for its multiple biological activities. In this study, a sensitive and selective method for determination of hinokiflavone in rat plasma was developed for the first time, using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Amentoflavone was used as an internal standard. Separation was achieved on a Hypersil Gold C18column with isocratic elution using methanol-water (65:35, v/v) as mobile phase at a flow rate of 0.3 mL/min. A triple quadrupole mass spectrometer operating in the negative electrospray mode with selected reaction monitoring was used to detect the transitions of m/z 537 → 284 for hinokiflavone and m/z 537 → 375 for IS. The LOQ was 0.9 ng/mL with a linear range of 0.9-1000 ng/mL. The intra- and inter-day accuracy (RE%) ranged from -3.75 to 6.91% and from -9.20 to 2.51% and the intra- and inter-day precision (RSD) was between 0.32-14.11 and 2.85-10.04%. The validated assay was successfully applied to a pharmacokinetic study of hinokiflavone in rats. The half-life of drug elimination at the terminal phase was 6.10 ± 1.86 h, and the area under the plasma concentration-time curve from time zero to the time of last measurable concentration and to infinity values obtained were 2394.42 ± 466.86 and 2541.93 ± 529.85 h ng/mL, respectively.
authors.affiliation: Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Ruofeng
authors.initials: R
authors.lastname: Yin
authors.affiliation: Department of Anatomy and Neurobiology, School of Basic Medical Science Central South University, Changsha, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xiong
authors.affiliation: Department of Clinical Epidemiology, the First Hospital of Jilin University, Changchun, China.
authors.forename: Simin
authors.initials: S
authors.lastname: Wen
authors.affiliation: Department of Spine Surgery, the First Hospital of Jilin University, Changchun, China.
authors.forename: Yuanyi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Spine Surgery, the First Hospital of Jilin University, Changchun, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
mesh_terms: D000818:Animals; D044946:Biflavonoids / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D044946:Biflavonoids; C060299:hinokiflavone
keywords: Hinokiflavone; LC-MS/MS; pharmacokinetics; rat plasma