title: Detection and Molecular Identification of Sarcocystis rileyi (Apicomplexa: Sarcocystidae) From a Northern Shoveler (Anas clypeata) in Mexico.
abstract: We detected macroscopic Sarcocystis cysts in a Northern Shoveler ( Anas clypeata ) in the Lerma Marshes, State of Mexico, Mexico in February 2014. The 5.0×2.0-mm macrocysts in the breast muscle of the duck were ovoid and yellow. Using an optical microscope, we saw parasitic forms of a Sarcocystis sp. among muscular fibers; the cysts measured 3.5×1.1 mm. The external wall had a smooth surface and the internal wall had a spongy texture. We identified the macrocysts as Sarcocystis rileyi according to sequences of the 18S rRNA gene, 28S rRNA gene, and ITS-1 region. Sarcocystosis should be considered in similar assessments in wild waterfowl in Mexico. Awareness of S. rileyi among anatids in the Lerma Marshes will contribute to more-effective conservation and management actions.
authors.affiliation: 1 Laboratorio de Diagnóstico Parasitológico, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, C.P. 04510, Distrito Federal, Mexico.
authors.forename: Patricia
authors.initials: P
authors.lastname: Padilla-Aguilar
authors.affiliation: 1 Laboratorio de Diagnóstico Parasitológico, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, C.P. 04510, Distrito Federal, Mexico.
authors.forename: Evangelina
authors.initials: E
authors.lastname: Romero-Callejas
authors.affiliation: 2 Laboratorio de Helmintología, Instituto de Biología, Universidad Nacional Autónoma de México, C.P. 04510, Distrito Federal, Mexico.
authors.forename: David
authors.initials: D
authors.lastname: Osorio-Sarabia
authors.affiliation: 3 Departamento de Patología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, C.P. 04510, Distrito Federal, Mexico.
authors.forename: José
authors.initials: J
authors.lastname: Ramírez-Lezama
authors.affiliation: 4 Laboratorio de Arbovirología, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Calle 43 No. 613 x Calle 90. Col. Inalámbrica, C.P. 97069, Mérida, Yucatán, Mexico.
authors.forename: Nohemi
authors.initials: N
authors.lastname: Cigarroa-Toledo
authors.affiliation: 4 Laboratorio de Arbovirología, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Calle 43 No. 613 x Calle 90. Col. Inalámbrica, C.P. 97069, Mérida, Yucatán, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Machain-Williams
authors.affiliation: 5 Grupo Anima Efferus A.C., Palenque No. 421-1, C.P. 03600, Distrito Federal, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Manterola
authors.affiliation: 6 Departamento de Ciencias Ambientales, CBS, Universidad Autónoma Metropolitana Unidad Lerma, Av. de las Garzas No. 10. Col. El Panteón, C.P. 52005, Lerma de Villada, State of Mexico, Mexico.
authors.forename: Heliot
authors.initials: H
authors.lastname: Zarza
mesh_terms: D000818:Animals; D004372:Ducks / Q000382:microbiology*; D008800:Mexico; D010802:Phylogeny; D012522:Sarcocystis / Q000302:isolation & purification* / Q000472:pathogenicity; D012523:Sarcocystosis / Q000662:veterinary
keywords: Anas clypeata; Lerma; Mexico; Northern Shoveler; Sarcocystis rileyi; parasite