title: Screening for Escherichia coli K-12 genes conferring glyoxal resistance or sensitivity by transposon insertions.
abstract: Glyoxal (GO) belongs to the reactive electrophilic species generated in vivo in all organisms. In order to identify targets of GO and their response mechanisms, we attempted to screen for GO-sensitive mutants by random insertions of TnphoA-132. The genes responsible for GO susceptibility were functionally classified as the following: (i) tRNA modification; trmE, gidA and truA, (ii) DNA repair; recA and recC, (iii) toxin-antitoxin; mqsA and (iv) redox metabolism; yqhD and caiC In addition, an insertion in the crp gene, encoding the cAMP responsive transcription factor, exhibits a GO-resistant phenotype, which is consistent with the phenotype of adenylate cyclase (cya) mutant showing GO resistance. This suggests that global regulation involving cAMP is operated in a stress response to GO. To further characterize the CRP-regulated genes directly associated with GO resistance, we created double mutants deficient in both crp and one of the candidate genes including yqhD, gloA and sodB The results indicate that these genes are negatively regulated by CRP as confirmed by real-time RT-PCR. We propose that tRNA as well as DNA are the targets of GO and that toxin/antitoxin, antioxidant and cAMP are involved in cellular response to GO.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Changhan
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Jihong
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Minsuk
authors.initials: M
authors.lastname: Kwon
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Kihyun
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Haeyoung
authors.initials: H
authors.lastname: Min
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Seong Hun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Dongkyu
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Nayoung
authors.initials: N
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Jiyeun
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Doyun
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Changmin
authors.initials: C
authors.lastname: Ko
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Republic of Korea ckpark@kaist.ac.kr.
authors.forename: Chankyu
authors.initials: C
authors.lastname: Park
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics; D002373:Cyclic AMP Receptor Protein / Q000235:genetics; D004251:DNA Transposable Elements; D024881:Drug Resistance, Bacterial / Q000235:genetics; D048168:Escherichia coli K12 / Q000187:drug effects* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics; D006037:Glyoxal / Q000378:metabolism / Q000494:pharmacology*; D016254:Mutagenesis, Insertional; D009154:Mutation; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D002373:Cyclic AMP Receptor Protein; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; C481662:crp protein, E coli; D006037:Glyoxal; D012343:RNA, Transfer; D000262:Adenylyl Cyclases
keywords: CRP; cAMP; glyoxal; methylglyoxal; tRNA