title: The activation of melanogenesis by p-CREB and MITF signaling with extremely low-frequency electromagnetic fields on B16F10 melanoma.
abstract: Melanin in the skin determines the skin color, and decreased melanin causes many hypopigmentation disorders and increased damage to skin by ultraviolet B (UVB) light irradiation. Here, we stimulate melanogenesis in B16F10 melanoma cells by using specific frequencies of ELF-EMFs. In this study, we focus on the melanogenesis of EMF-ELFs and find that 60-75Hz ELF-EMFs upregulate melanin synthesis by stimulated expression of tyrosinase and TRP-1 through inhibition of phosphorylation ERK, activation of CREB, and MITF up-regulation in B16F10 melanoma cells. The results show that 60-75Hz ELF-EMFs significantly increase secreted melanin, cellular melanin content, and tyrosinase activity, and the cell mitochondria activity, cell viability, and cell membrane condition are unchanged. Furthermore, the protein expression level of MITF and p-CREB signaling pathway are significantly increased. Moreover, 60Hz ELF-EMFs reduce the phosphorylate of ERK in B16F10 melanoma cells. These findings indicate that stimulation of melanogenesis by using ELF-EMFs has therapeutic potential for treating hypopigmentation disorders such as vitiligo.
authors.affiliation: Department of Medical Biotechnology (BK21 Plus team), Dongguk University, Seoul 100-715, Republic of Korea.
authors.forename: Yu-Mi
authors.initials: YM
authors.lastname: Kim
authors.affiliation: Department of Medical Biotechnology (BK21 Plus team), Dongguk University, Seoul 100-715, Republic of Korea.
authors.forename: Sang-Eun
authors.initials: SE
authors.lastname: Cho
authors.affiliation: Department of Medical Biotechnology (BK21 Plus team), Dongguk University, Seoul 100-715, Republic of Korea. Electronic address: bioseo@dongguk.edu.
authors.forename: Young-Kwon
authors.initials: YK
authors.lastname: Seo
mesh_terms: D000818:Animals; D050882:CREB-Binding Protein / Q000378:metabolism*; D045744:Cell Line, Tumor; D004574:Electromagnetic Fields; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis* / Q000378:metabolism; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; C497235:Mitf protein, mouse; D007770:L-Lactate Dehydrogenase; D014442:Monophenol Monooxygenase; D050882:CREB-Binding Protein; C494364:Crebbp protein, mouse
keywords: B16F10 melanoma; Extremely low-frequency electromagnetic fields (ELF-EMFs); MITF; Melanogenesis; p-CREB