title: Tuning the Binding Affinities and Reversion Kinetics of a Light Inducible Dimer Allows Control of Transmembrane Protein Localization.
abstract: Inducible dimers are powerful tools for controlling biological processes through colocalizing signaling molecules. To be effective, an inducible system should have a dissociation constant in the "off" state that is greater (i.e., weaker affinity) than the concentrations of the molecules that are being controlled, and in the "on" state a dissociation constant that is less (i.e., stronger affinity) than the relevant protein concentrations. Here, we reengineer the interaction between the light inducible dimer, iLID, and its binding partner SspB, to better control proteins present at high effective concentrations (5-100 μM). iLID contains a light-oxygen-voltage (LOV) domain that undergoes a conformational change upon activation with blue light and exposes a peptide motif, ssrA, that binds to SspB. The new variant of the dimer system contains a single SspB point mutation (A58V), and displays a 42-fold change in binding affinity when activated with blue light (from 3 ± 2 μM to 125 ± 40 μM) and allows for light-activated colocalization of transmembrane proteins in neurons, where a higher affinity switch (0.8-47 μM) was less effective because more colocalization was seen in the dark. Additionally, with a point mutation in the LOV domain (N414L), we lengthened the reversion half-life of iLID. This expanded suite of light induced dimers increases the variety of cellular pathways that can be targeted with light.
authors.forename: Seth P
authors.initials: SP
authors.lastname: Zimmerman
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Hallett
authors.affiliation: Department of Pharmacology, University of Colorado School of Medicine , Aurora, Colorado 80045, United States.
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Bourke
authors.forename: James E
authors.initials: JE
authors.lastname: Bear
authors.affiliation: Department of Pharmacology, University of Colorado School of Medicine , Aurora, Colorado 80045, United States.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Kennedy
authors.forename: Brian
authors.initials: B
authors.lastname: Kuhlman
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019281:Dimerization; D007700:Kinetics; D008027:Light; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice
chemical_list: D008565:Membrane Proteins