title: The Beneficial Effects of Renal Transplantation on Altered Oxidative Status of ESRD Patients.
abstract: Renal transplantation (RT), has been considered the best therapeutic option for end stage renal disease (ESRD). Objective. To determine the effect of RT on the evolution of oxidative DNA status. Methods. Prospective cohort (N = 50 receptors of RT); genotoxic damage, 8-hydroxy-2'-deoxyguanosine (8-OHdG), and DNA repair enzyme, human 8-oxoguanine-DNA-N- glycosylase-1 (hOGG1); and antioxidants, superoxide dismutase (SOD) and glutathione peroxidase (GPx), were evaluated. Results. Before RT, 8-OHdG were significantly elevated (11.04 ± 0.90 versus 4.73 ± 0.34 ng/mL) compared to healthy controls (p = 0.001), with normalization after 6 months of 4.78 ± 0.34 ng/mL (p < 0.001). The same phenomenon was observed with hOGG1 enzyme before RT with 2.14 ± 0.36 ng/mL (p = 0.01) and decreased significantly at the end of the study to 1.20 ng/mL (p < 0.001) but was higher than controls, 0.51 ± 0.07 ng/mL (p < 0.03). Antioxidant SOD was elevated at 24.09 ± 1.6 IU/mL versus healthy controls (p = 0.001) before RT; however, 6 months after RT it decreased significantly to 16.9 ± 1.6 IU/mL (p = 0.002), without achieving the levels of healthy controls (p = 0.01). The GPx, before RT, was significantly diminished with 24.09 ± 1.6 IU/mL versus healthy controls (39.0 ± 1.58) (p = 0.01), while, in the final results, levels increased significantly to 30.38 ± 3.16 IU/mL (p = 0.001). Discussion. Patients with ESRD have important oxidative damage before RT. The RT significantly reduces oxidative damage and partially regulates the antioxidant enzymes (SOD and GPx).
authors.affiliation: Department of Nephrology and Transplants, Specialties Hospital, National Occidental Medical Centre, The Mexican Social Security Institute, Guadalajara, 44349 Guadalajara, JAL, Mexico.
authors.forename: José Ignacio
authors.initials: JI
authors.lastname: Cerrillos-Gutiérrez
authors.affiliation: Department of Physiology, University Health Sciences Centre, The University of Guadalajara, Guadalajara, 44150 Guadalajara, JAL, Mexico.
authors.forename: Alejandra Guillermina
authors.identifier: 0000-0002-3635-6135
authors.initials: AG
authors.lastname: Miranda-Díaz
authors.affiliation: Department of Nephrology and Transplants, Specialties Hospital, National Occidental Medical Centre, The Mexican Social Security Institute, Guadalajara, 44349 Guadalajara, JAL, Mexico.
authors.forename: Priscila
authors.initials: P
authors.lastname: Preciado-Rojas
authors.affiliation: Department of Nephrology and Transplants, Specialties Hospital, National Occidental Medical Centre, The Mexican Social Security Institute, Guadalajara, 44349 Guadalajara, JAL, Mexico.
authors.forename: Benjamín
authors.initials: B
authors.lastname: Gómez-Navarro
authors.affiliation: Department of Physiology, University Health Sciences Centre, The University of Guadalajara, Guadalajara, 44150 Guadalajara, JAL, Mexico.
authors.forename: Sonia
authors.initials: S
authors.lastname: Sifuentes-Franco
authors.affiliation: Department of Physiology, University Health Sciences Centre, The University of Guadalajara, Guadalajara, 44150 Guadalajara, JAL, Mexico.
authors.forename: Sandra
authors.initials: S
authors.lastname: Carrillo-Ibarra
authors.affiliation: Department of Nephrology and Transplants, Specialties Hospital, National Occidental Medical Centre, The Mexican Social Security Institute, Guadalajara, 44349 Guadalajara, JAL, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Andrade-Sierra
authors.affiliation: Kidney Diseases Medical Research Unit, Specialties Hospital, National Occidental Medical Centre, Mexican Social Security Institute, Guadalajara, 44349 Guadalajara, JAL, Mexico.
authors.forename: Enrique
authors.initials: E
authors.lastname: Rojas-Campos
authors.affiliation: Kidney Diseases Medical Research Unit, Specialties Hospital, National Occidental Medical Centre, Mexican Social Security Institute, Guadalajara, 44349 Guadalajara, JAL, Mexico.
authors.forename: Alfonso Martín
authors.initials: AM
authors.lastname: Cueto-Manzano
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D045647:DNA Glycosylases / Q000097:blood; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood*; D016030:Kidney Transplantation; D008297:Male; D018384:Oxidative Stress
chemical_list: D000975:Antioxidants; D000080242:8-Hydroxy-2'-Deoxyguanosine; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human; D003849:Deoxyguanosine