title: Ovarian follicular dynamics after aromatizable or non aromatizable neonatal androgenization.
abstract: The effects of neonatal testosterone or dihydrotestosterone exposure on ovarian follicular dynamics were analysed at prepubertal, pubertal or adult age in Wistar rats. Both androgens induced a transitory increase on follicular endowment that was partially corrected at puberty. At adult age testosterone prevented ovulation, without significant modifications on follicular dynamics. An increased number of cystic structures were observed from puberty to adult age. However, ovaries of rats treated with dihydrotestosterone showed follicles with evident morphological alterations in granulosa and thecal layers although several corpora lutea were observed. A significant increase in preantral follicles and few cystic structures were detected at advanced adulthood. The size of cyst increased with age. No immunohistochemical changes on growth factors or enzymes related to steroidogenesis in growing follicles were obvious in any group. In both androgenized groups, cysts shared immunohistochemical characteristics exhibited by preovulatory follicles but they were unable to ovulate spontaneously. Our results provide an insight into the role of different androgens in female reproductive system development, indicating a direct effect of dihydrotestosterone on ovarian tissues whereas a central effect would be the main feature of neonatal testosterone exposure. Heterogeneous clinical manifestations seen in pathologies such as polycystic ovary syndrome among women could be associated with subtle hormonal changes during follicular population development.
authors.affiliation: Histology and Embryology Department, School of Medicine, General Flores 2125, CP 11800, Montevideo, Uruguay. ganesett@fmed.edu.uy.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Anesetti
authors.affiliation: Histology and Embryology Department, School of Medicine, General Flores 2125, CP 11800, Montevideo, Uruguay.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Chávez-Genaro
mesh_terms: D000728:Androgens / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers; D013196:Dihydrotestosterone / Q000378:metabolism; D030762:Estrous Cycle; D005260:Female; D007150:Immunohistochemistry; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology*; D010053:Ovary / Q000166:cytology / Q000502:physiology; D051381:Rats; D013739:Testosterone / Q000378:metabolism
chemical_list: D000728:Androgens; D015415:Biomarkers; D013196:Dihydrotestosterone; D013739:Testosterone
keywords: Androgen; Cyst; Ovary