title: Anti-inflammatory and anti-fibrotic effects of annexin1 on erectile function after cavernous nerve injury in rats.
abstract: The aim of this study was to investigate the effect of the anti-inflammatory and anti-fibrotic actions of ANX1 on erectile function (EF). Forty-eight male Wistar rats were randomly distributed into four equal groups: one group (sham operation-control) and three groups (bilateral cavernous nerve (CN) crush injury). Crush injury groups were treated prior to injury with an intravascular injection of either ANX1 (50 or 100 μg kg-1) or vehicle. EF was assessed by CN electrical stimulation at 2 and 7 days after CN injury with histomorphometric and immunohistochemical analysis. ANX1 demonstrated functional preservation as the increase in intracavernous pressure (ICP). A dose-response relationship regarding the effect on penile tissue was confirmed, and preservation of the penile dorsal nerves and anti-apoptotic effects in the corpus cavernosum (real P-value vs injured control). ANX1 treatment prevented collagen deposition and smooth muscle loss in the penis. ANX1 normalized the expression of vascular endothelial growth factor and decreased tumor necrosis factor-α in the lumen of the blood vessels of the organ. ANX1 proved effective in preserving EF in a rat model of neurogenic erectile dysfunction. ANX1 treatment before CN injury in rats improved erectile recovery, enhanced vascular regeneration and preserved the micro-architecture of the corpus cavernosum. The clinical availability of this compound merits application in penile rehabilitation studies following radical prostatectomy.
authors.affiliation: Division of Urology, Medical School of São José do Rio Preto (FAMERP), Sao Paulo, Brazil.
authors.forename: F N
authors.initials: FN
authors.lastname: Facio
authors.affiliation: Department of Medicine, Undergraduate Student, Ceres School of Medicine, Faceres, Brazil.
authors.forename: M F
authors.initials: MF
authors.lastname: Facio
authors.affiliation: Division of Urology, Medical School of São José do Rio Preto (FAMERP), Sao Paulo, Brazil.
authors.forename: L F
authors.initials: LF
authors.lastname: Spessoto
authors.affiliation: Division of Urology, Medical School of São José do Rio Preto (FAMERP), Sao Paulo, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Pessutti
authors.affiliation: Department of Urology, Faculty of Medicine, Division of Urology, Center for Life Sciences, Pontifical Catholic University of Campinas, PUC-Campinas, Campinas, Brazil.
authors.forename: L O
authors.initials: LO
authors.lastname: Reis
authors.affiliation: Department of Biology, São Paulo State University, UNESP/IBILCE, São José Rio Preto, Brazil.
authors.forename: S G
authors.initials: SG
authors.lastname: Campos
authors.affiliation: Department of Biology, São Paulo State University, UNESP/IBILCE, São José Rio Preto, Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Taboga
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000494:pharmacology* / Q000627:therapeutic use; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D007172:Erectile Dysfunction / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D009409:Nerve Crush; D010410:Penile Erection / Q000187:drug effects* / Q000502:physiology; D010413:Penis / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D059348:Peripheral Nerve Injuries / Q000150:complications* / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D017305:Annexin A1; D000893:Anti-Inflammatory Agents; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A