title: Cytotoxicity and the effect of cationic peptide fragments against cariogenic bacteria under planktonic and biofilm conditions.
abstract: This study evaluated the cytotoxicity and effect of fragments derived from three oral cationic peptides (CP): LL-37, D6-17 and D1-23 against cariogenic bacteria under planktonic and biofilm conditions. For cytotoxicity analysis, two epithelial cell lines were used. The minimum inhibitory concentration and the minimal bactericidal concentration were determined for the CP fragments and the control (chlorhexidine-CHX) against cariogenic bacteria. The fractional inhibitory concentration was obtained for the combinations of CP fragments on Streptococcus mutans. Biofilm assays were conducted with the best antimicrobial CP fragment against S. mutans. The results indicated that D6-17 was not cytotoxic. D1-23, LL-37 and CHX were not cytotoxic in low concentrations. D1-23 presented the best bactericidal activity against S. mutans, S. mitis and S. salivarius. Combinations of CP fragments did not show a synergic effect. D1-23 presented a higher activity against S. mutans biofilm than CHX. It was concluded that D1-23 showed a substantial effect against cariogenic bacteria and low cytotoxicity.
authors.affiliation: a Department of Pediatric Dentistry and Public Health, Araçatuba Dental School , UNESP - Universidade Estadual Paulista , Araçatuba , São Paulo , Brazil.
authors.forename: Paula Fernanda
authors.initials: PF
authors.lastname: Kreling
authors.affiliation: a Department of Pediatric Dentistry and Public Health, Araçatuba Dental School , UNESP - Universidade Estadual Paulista , Araçatuba , São Paulo , Brazil.
authors.forename: Kelly Limi
authors.initials: KL
authors.lastname: Aida
authors.affiliation: b Department of Restorative Dentistry (Endodontics) , Araçatuba Dental School, UNESP - Universidade Estadual Paulista , Araçatuba , São Paulo , Brazil.
authors.forename: Loiane
authors.initials: L
authors.lastname: Massunari
authors.affiliation: b Department of Restorative Dentistry (Endodontics) , Araçatuba Dental School, UNESP - Universidade Estadual Paulista , Araçatuba , São Paulo , Brazil.
authors.forename: Karina Sampaio
authors.initials: KS
authors.lastname: Caiaffa
authors.affiliation: a Department of Pediatric Dentistry and Public Health, Araçatuba Dental School , UNESP - Universidade Estadual Paulista , Araçatuba , São Paulo , Brazil.
authors.forename: Célio
authors.initials: C
authors.lastname: Percinoto
authors.affiliation: c Department of Physiology and Pathology , Araraquara Dental School, UNESP - Universidade Estadual Paulista , Araraquara , São Paulo , Brazil.
authors.forename: Telma Blanca Lombardo
authors.initials: TB
authors.lastname: Bedran
authors.affiliation: c Department of Physiology and Pathology , Araraquara Dental School, UNESP - Universidade Estadual Paulista , Araraquara , São Paulo , Brazil.
authors.forename: Denise Madalena Palomari
authors.initials: DM
authors.lastname: Spolidorio
authors.affiliation: d Department of Dental Materials , Piracicaba Dental School, UNICAMP - Universidade de Campinas , Piracicaba , São Paulo , Brazil.
authors.forename: Gabriel Flores
authors.initials: GF
authors.lastname: Abuna
authors.affiliation: e Department of Biochemistry and Chemical Technology , Institute of Chemistry, UNESP - Universidade Estadual Paulista , Araraquara , São Paulo , Brazil.
authors.forename: Eduardo Maffud
authors.initials: EM
authors.lastname: Cilli
authors.affiliation: a Department of Pediatric Dentistry and Public Health, Araçatuba Dental School , UNESP - Universidade Estadual Paulista , Araçatuba , São Paulo , Brazil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: Duque
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000633:toxicity; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology* / Q000633:toxicity; D018441:Biofilms / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002710:Chlorhexidine / Q000494:pharmacology / Q000633:toxicity; D003731:Dental Caries / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D010446:Peptide Fragments / Q000494:pharmacology* / Q000633:toxicity; D010933:Plankton / Q000187:drug effects*; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D010446:Peptide Fragments; D002710:Chlorhexidine
keywords: Dental caries; biofilms; cell culture; peptides