title: Analysis of RPE morphometry in human eyes.
abstract: To describe the RPE morphometry of healthy human eyes regarding age and topographic location using modern computational methods with high accuracy and objectivity. We tested whether there were regional and age-related differences in RPE cell area and shape.
  
  
  
  Human cadaver donor eyes of varying ages were dissected, and the RPE flatmounts were immunostained for F-actin with AF635-phalloidin, nuclei stained with propidium iodide, and imaged with confocal microscopy. Image analysis was performed using ImageJ (NIH) and CellProfiler software. Quantitative parameters, including cell density, cell area, polygonality of cells, number of neighboring cells, and measures of cell shape, were obtained from these analyses to characterize individual and groups of RPE cells. Measurements were taken from selected areas spanning the length of the temporal retina through the macula and the mid-periphery to the far periphery.
  
  
  
  Nineteen eyes from 14 Caucasian donors of varying ages ranging from 29 to 80 years were used. Along a horizontal nasal to temporal meridian, there were differences in several cell shape and size characteristics. Generally, the cell area and shape was relatively constant and regular except in the far periphery. In the outer third of the retina, the cell area and shape differed from the inner two-thirds statistically significantly. In the macula and the far periphery, an overall decreasing trend in RPE cell density, percent hexagonal cells, and form factor was observed with increasing age. We also found a trend toward increasing cell area and eccentricity with age in the macula and the far periphery. When individuals were divided into two age groups, <60 years and ≥60 years, there was a higher cell density, lower cell area, lower eccentricity, and higher form factor in the younger group in the macula and the far periphery (p<0.05 for all measurements). No statistically significant differences in RPE morphometry between age groups were found in the mid-periphery.
  
  
  
  Human cadaver RPE cells differ mainly in area and shape in the outer one third compared to the inner two-thirds of the temporal retina. RPE cells become less dense and larger, lose their typical hexagonal shape, and become more oval with increasing age.
authors.affiliation: Department of Ophthalmology, School of Medicine, School of Medicine, Emory University, Atlanta, GA.
authors.forename: Shagun K
authors.initials: SK
authors.lastname: Bhatia
authors.affiliation: Department of Ophthalmology, School of Medicine, School of Medicine, Emory University, Atlanta, GA.
authors.forename: Alia
authors.initials: A
authors.lastname: Rashid
authors.affiliation: Department of Ophthalmology, School of Medicine, School of Medicine, Emory University, Atlanta, GA.
authors.forename: Micah A
authors.initials: MA
authors.lastname: Chrenek
authors.affiliation: Department of Ophthalmology, School of Medicine, School of Medicine, Emory University, Atlanta, GA.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, School of Medicine, School of Medicine, Emory University, Atlanta, GA; Department of Epidemiology, Rollins School of Public Health, Emory University, Atlanta, GA; Department of Neurology, School of Medicine, Emory University, Atlanta, GA.
authors.forename: Beau B
authors.initials: BB
authors.lastname: Bruce
authors.affiliation: Department of Epidemiology, Rollins School of Public Health, Emory University, Atlanta, GA; Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA.
authors.forename: Mitchel
authors.initials: M
authors.lastname: Klein
authors.affiliation: Department of Ophthalmology, School of Medicine, School of Medicine, Emory University, Atlanta, GA; Center for Visual and Neurocognitive Rehabilitation, Atlanta VA Medical Center, Decatur, GA.
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Boatright
authors.affiliation: Department of Mathematics and Statistics, Georgia State University, Atlanta, GA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Ophthalmology, School of Medicine, School of Medicine, Emory University, Atlanta, GA.
authors.forename: Hans E
authors.initials: HE
authors.lastname: Grossniklaus
authors.affiliation: Department of Ophthalmology, School of Medicine, School of Medicine, Emory University, Atlanta, GA.
authors.forename: John M
authors.initials: JM
authors.lastname: Nickerson
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D002452:Cell Count; D048430:Cell Shape / Q000502:physiology*; D048429:Cell Size; D005260:Female; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D055213:Retinal Pigment Epithelium / Q000166:cytology*; D014019:Tissue Donors; D044465:White People