title: [Action Mechanism of Chlorambucil against Mantle Cell Lymphoma Cell Line Jeko-1].
abstract: OBJECTIVE
  To explore the action mechanism of chlorambucil against mantle cell lymphoma cell line Jeko-1.
  
  
  METHODS
  The effect of chlorambucil on Jeko-1 cell proliferation was measured by MTT method. The effect of chlorambucil on the apoptosis of Jeko-1 cell was detected by Hoechst staining and Annexin V-FITC dual staining. The activation of PI3K/AKT signaling pathway and the expression of BAX, BCL-2, procaspase 3, procaspase 8 and procaspase 9 were detected by Western blot.
  
  
  RESULTS
  0, 5, 10, 20 µmol/L chlorambucil could inhibit Jeko-1 cell proliferation at 24, 48, 72 h in a time- and dose-dependent manner. Chlorambucil of 0, 5, 10, 20 µmol/L increased the apoptotic rate of Jeko-1 cells, upregulated the expression of BAX, procaspase 3, procaspase 8, procaspase 9 and PI3K, increased the phosphorylation of AKT and down-regulated the expression of BCL-2.
  
  
  CONCLUSIONS
  The chlorambucil can induce the apoptosis of mantle cell lymphoma Jeko-1 cells via blocking PI3K/AKT signaling pathway.
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Guo
authors.affiliation: Department of Hemodialysis, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.
authors.forename: Ya-Hong
authors.initials: YH
authors.lastname: Zhou
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.
authors.forename: Jing-Xin
authors.initials: JX
authors.lastname: Pan
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.
authors.forename: Xi-Zhe
authors.initials: XZ
authors.lastname: Guo
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.
authors.forename: Shi-Xin
authors.initials: SX
authors.lastname: Wu
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002699:Chlorambucil; D015536:Down-Regulation; D006801:Humans; D020522:Lymphoma, Mantle-Cell; D019869:Phosphatidylinositol 3-Kinases; D010766:Phosphorylation; D015398:Signal Transduction
chemical_list: D002699:Chlorambucil; D019869:Phosphatidylinositol 3-Kinases