title: Methods of Hematoxylin and Erosin Image Information Acquisition and Optimization in Confocal Microscopy.
abstract: OBJECTIVE
  We produced hematoxylin and eosin (H&E) staining-like color images by using confocal laser scanning microscopy (CLSM), which can obtain the same or more information in comparison to conventional tissue staining.
  
  
  METHODS
  We improved images by using several image converting techniques, including morphological methods, color space conversion methods, and segmentation methods.
  
  
  RESULTS
  An image obtained after image processing showed coloring very similar to that in images produced by H&E staining, and it is advantageous to conduct analysis through fluorescent dye imaging and microscopy rather than analysis based on single microscopic imaging.
  
  
  CONCLUSIONS
  The colors used in CLSM are different from those seen in H&E staining, which is the method most widely used for pathologic diagnosis and is familiar to pathologists. Computer technology can facilitate the conversion of images by CLSM to be very similar to H&E staining images. We believe that the technique used in this study has great potential for application in clinical tissue analysis.
authors.affiliation: Biomedical Engineering Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea.
authors.forename: Woong Bae
authors.initials: WB
authors.lastname: Yoon
authors.affiliation: Molecular Imaging & Therapy Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea.
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Kim
authors.affiliation: Biomedical Engineering Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea.
authors.forename: Kwang Gi
authors.initials: KG
authors.lastname: Kim
authors.affiliation: Molecular Imaging & Therapy Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea.
authors.forename: Yongdoo
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Colorectal Cancer Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea.
authors.forename: Hee Jin
authors.initials: HJ
authors.lastname: Chang
authors.affiliation: Biomedical Engineering Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea.; Colorectal Cancer Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea.
authors.forename: Dae Kyung
authors.initials: DK
authors.lastname: Sohn
keywords: Computer-Assisted Image Processing; Confocal Laser Scanning Microscopy; Fluorescence; Hematoxylin; Histological Labeling