title: Human satellite-III non-coding RNAs modulate heat-shock-induced transcriptional repression.
abstract: The heat shock response is a conserved defense mechanism that protects cells from physiological stress, including thermal stress. Besides the activation of heat-shock-protein genes, the heat shock response is also known to bring about global suppression of transcription; however, the mechanism by which this occurs is poorly understood. One of the intriguing aspects of the heat shock response in human cells is the transcription of satellite-III (Sat3) long non-coding RNAs and their association with nuclear stress bodies (nSBs) of unknown function. Besides association with the Sat3 transcript, the nSBs are also known to recruit the transcription factors HSF1 and CREBBP, and several RNA-binding proteins, including the splicing factor SRSF1. We demonstrate here that the recruitment of CREBBP and SRSF1 to nSBs is Sat3-dependent, and that loss of Sat3 transcripts relieves the heat-shock-induced transcriptional repression of a few target genes. Conversely, forced expression of Sat3 transcripts results in the formation of nSBs and transcriptional repression even without a heat shock. Our results thus provide a novel insight into the regulatory role for the Sat3 transcripts in heat-shock-dependent transcriptional repression.
authors.affiliation: Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, India.
authors.forename: Anshika
authors.initials: A
authors.lastname: Goenka
authors.affiliation: Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, India.
authors.forename: Sonali
authors.initials: S
authors.lastname: Sengupta
authors.affiliation: CSIR Ayurgenomics Unit - TRISUTRA, CSIR - Institute of Genomics and Integrative Biology (IGIB), Mathura Road, New Delhi 110025, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Pandey
authors.affiliation: Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, India.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Parihar
authors.affiliation: Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, India.
authors.forename: Girish Chandra
authors.initials: GC
authors.lastname: Mohanta
authors.affiliation: CSIR Ayurgenomics Unit - TRISUTRA, CSIR - Institute of Genomics and Integrative Biology (IGIB), Mathura Road, New Delhi 110025, India.
authors.forename: Mitali
authors.initials: M
authors.lastname: Mukerji
authors.affiliation: Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, India sganesh@iitk.ac.in.
authors.forename: Subramaniam
authors.identifier: 0000-0002-9908-9177
authors.initials: S
authors.lastname: Ganesh
mesh_terms: D050882:CREB-Binding Protein / Q000378:metabolism; D016923:Cell Death; D002467:Cell Nucleus / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D018869:Heat-Shock Response / Q000235:genetics*; D006801:Humans; D008954:Models, Biological; D011485:Protein Binding / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D000068103:Serine-Arginine Splicing Factors / Q000378:metabolism; D013312:Stress, Physiological; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D022661:RNA, Untranslated; C000595779:SRSF1 protein, human; D000068103:Serine-Arginine Splicing Factors; D050882:CREB-Binding Protein; C494363:CREBBP protein, human
keywords: Heat shock response; Non-coding RNA; Nuclear stress bodies; Transcription factor