title: Ets2 knockdown inhibits tumorigenesis in esophageal squamous cell carcinoma in vivo and in vitro.
abstract: Increased expression of Ets2 is reported upregulated in esophageal squamous cell carcinoma tissue. However, the function of Ets2 in carcinogenesis of ESCC is poorly understood. Here, the rise of Ets2 was confirmed in ESCC cells and Ets2 depletion by RNA interference promotes cell apoptosis, inhibits cell proliferation, attenuates cell invasion and induces cell cycle G0/G1 arrest in vitro. Moreover, in vivo, Xenograft mouse model studies showed Ets2 knockdown inhibits tumor formation and metastasis significantly. Furthermore, Ets2 depletion inactivates the mTOR/p70S6K signaling pathway both in vitro and in vivo. Taken together, these findings strongly suggest that a critical role of Ets2 in human ESCC pathogenesis via the inactivation of the mTOR/p70S6K signaling pathway.
authors.affiliation: The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.|School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Han
authors.affiliation: The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, Henan Province, China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Zhu
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Ma
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.|School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Fangxia
authors.initials: F
authors.lastname: Guan
mesh_terms: D000818:Animals; D017209:Apoptosis; D063646:Carcinogenesis / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D004938:Esophageal Neoplasms / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D059585:G1 Phase Cell Cycle Checkpoints; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D050787:Proto-Oncogene Protein c-ets-2 / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism*; D015398:Signal Transduction; D013047:Specific Pathogen-Free Organisms; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D015854:Up-Regulation; D023041:Xenograft Model Antitumor Assays
chemical_list: C494069:ETS2 protein, human; D050787:Proto-Oncogene Protein c-ets-2; D034741:RNA, Small Interfering; C546842:MTOR protein, human; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D058570:TOR Serine-Threonine Kinases
keywords: Ets2; apoptosis; esophageal squamous cell carcinoma; mTOR/p70S6K signaling pathway; proliferation