title: Promotion of adhesion and proliferation of endothelial progenitor cells on decellularized valves by covalent incorporation of RGD peptide and VEGF.
abstract: Tissue engineered heart valve is a promising alternative to current heart valve surgery, for its capability of growth, repair, and remodeling. However, extensive development is needed to ensure tissue compatibility, durability and antithrombotic potential. This study aims to investigate the biological effects of multi-signal composite material of polyethyl glycol-cross-linked decellularized valve on adhesion and proliferation of endothelial progenitor cells. Group A to E was decellularized valve leaflets, composite material of polyethyl glycol-cross-linked decellularized valves leaflets, vascular endothelial growth factor-composite materials, Arg-Gly-Asp peptide-composite materials and multi-signal modified materials of polyethyl glycol-cross-linked decellularized valve leaflets, respectively. The endothelial progenitor cells were seeded for each group, cell adhesion and proliferation were detected and neo-endothelium antithrombotic function of the multi-signal composite materials was evaluated. At 2, 4, and 8 h after the seeding, the cell numbers and 3H-TdR incorporation in group D were the highest. At 2, 4, and 8 days after the seeding, the cell numbers and 3H-TdR incorporation were significantly higher in groups C, D, and E compared with groups A and B (P < 0.05) and cell numbers and the expression of t-PA and eons in the neo-endothelium were quite similar to those in the human umbilical vein endothelial cells at 2, 4, and 8 days after the seeding. The Arg-Gly-Asp- peptides (a sequential peptide composed of arginine (Arg), glycine (Gly) and aspartic acid (Asp)) and VEGF-conjugated onto the composite material of PEG-crosslinked decellularized valve leaflets synergistically promoted the adhesion and proliferation of endothelial progenitor cells on the composite material, which may help in tissue engineering of heart valves.
authors.affiliation: Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China. zhoujianliang2010@163.com.
authors.forename: Jianliang
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Jingli
authors.initials: J
authors.lastname: Ding
authors.affiliation: Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
authors.forename: Bin'en
authors.initials: B
authors.lastname: Nie
authors.affiliation: Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Shidong
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Cardiovascular Surgery, the Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Cardiovascular Surgery, the Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430022, China. dongnianguo2013@163.com.
authors.forename: Nianguo
authors.initials: N
authors.lastname: Dong
mesh_terms: D000818:Animals; D001120:Arginine / Q000737:chemistry; D001224:Aspartic Acid / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D003432:Cross-Linking Reagents / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D005343:Fibrinolytic Agents / Q000737:chemistry; D005998:Glycine / Q000737:chemistry; D006351:Heart Valves / Q000187:drug effects* / Q000473:pathology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry; D008297:Male; D009842:Oligopeptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering / Q000379:methods*; D010959:Tissue Plasminogen Activator / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D005343:Fibrinolytic Agents; D008077:Lipoproteins, LDL; D009842:Oligopeptides; D042461:Vascular Endothelial Growth Factor A; D001224:Aspartic Acid; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; D001120:Arginine; D004492:Edetic Acid; D010959:Tissue Plasminogen Activator; D005998:Glycine