title: A plasmonic colorimetric strategy for visual miRNA detection based on hybridization chain reaction.
abstract: In this work, a novel colorimetric strategy for miRNA analysis is proposed based on hybridization chain reaction (HCR)-mediated localized surface plasmon resonance (LSPR) variation of silver nanoparticles (AgNPs). miRNA in the sample to be tested is able to release HCR initiator from a solid interface to AgNPs colloid system by toehold exchange-mediated strand displacement, which then triggers the consumption of fuel strands with single-stranded tails for HCR. The final produced long nicked double-stranded DNA loses the ability to protect AgNPs from salt-induced aggregation. The stability variation of the colloid system can then be monitored by recording corresponding UV-vis spectrum and initial miRNA level is thus determined. This sensing system involves only four DNA strands which is quite simple. The practical utility is confirmed to be excellent by employing different biological samples.
authors.affiliation: Department of Clinical Laboratory, the 404th Hospital of PLA, Weihai 264200, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Miao
authors.affiliation: Department of Clinical Laboratory, the 404th Hospital of PLA, Weihai 264200, P. R. China.
authors.forename: Jingsheng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, the 404th Hospital of PLA, Weihai 264200, P. R. China.
authors.forename: Jinyang
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Clinical Laboratory, the 404th Hospital of PLA, Weihai 264200, P. R. China.
authors.forename: Huiguang
authors.initials: H
authors.lastname: Gao
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, P. R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Han
authors.affiliation: Department of Chemistry, Rice University, Houston, Texas 77005, United States.
authors.forename: Chengmin
authors.initials: C
authors.lastname: Jiang
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, P. R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Miao
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry; D006046:Gold; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000472:pathogenicity; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D035683:MicroRNAs / Q000032:analysis*; D046529:Microscopy, Electron, Transmission; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D004277:DNA, Single-Stranded; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D012834:Silver; D006046:Gold