title: Response of cbb gene transcription levels of four typical sulfur-oxidizing bacteria to the CO2 concentration and its effect on their carbon fixation efficiency during sulfur oxidation.
abstract: The variability in carbon fixation capability of four sulfur-oxidizing bacteria (Thiobacillus thioparus DSM 505, Halothiobacillus neapolitanus DSM 15147, Starkeya novella DSM 506, and Thiomonas intermedia DSM 18155) during sulfur oxidation was studied at low and high concentrations of CO2. The mechanism underlying the variability in carbon fixation was clarified by analyzing the transcription of the cbb gene, which encodes the key enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase. DSM 15147 and DSM 505 fixed carbon more efficiently during sulfur oxidation than DSM 506 and DSM 18155 at 0.5% and 10% CO2, which was mainly because their cbb gene transcription levels were much higher than those of DSM 506 and DSM 18155. A high CO2 concentration significantly stimulated the carbon fixation efficiency of DSM 505 by greatly increasing the cbb gene transcription efficiency. Moreover, the influence of the CO2 concentration on the carbon fixation efficiency of the four strains differed greatly during sulfur oxidation.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Collaborative Innovation Center for Regional Environmental Quality, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Collaborative Innovation Center for Regional Environmental Quality, China. Electronic address: celwang@tongji.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Science and Environmental Studies, The Education University of Hong Kong, Hong Kong, China. Electronic address: tsangyf@eduhk.hk.
authors.forename: Yiu Fai
authors.initials: YF
authors.lastname: Tsang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Collaborative Innovation Center for Regional Environmental Quality, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Fu
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Collaborative Innovation Center for Regional Environmental Quality, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Collaborative Innovation Center for Regional Environmental Quality, China.
authors.forename: Yiquan
authors.initials: Y
authors.lastname: Le
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D020562:Betaproteobacteria / Q000235:genetics / Q000378:metabolism; D057486:Carbon Cycle / Q000235:genetics; D002245:Carbon Dioxide / Q000378:metabolism; D005798:Genes, Bacterial; D044222:Halothiobacillus / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism*; D013855:Thiobacillus / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002245:Carbon Dioxide; D013455:Sulfur; D012273:Ribulose-Bisphosphate Carboxylase
keywords: CO(2) concentration; Carbon fixation; Sulfur-oxidizing bacteria; cbb gene transcription level