title: Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis.
abstract: The efficiency of miniaturized liquid chromatography columns with inner diameters between 200 and 300 μm has been investigated using a dedicated micro-liquid chromatography system. Fully porous, core-shell and monolithic commercially available stationary phases were compared applying van Deemter and kinetic plot analysis. The sub-2 μm fully porous as well as the 2.7 μm core-shell particle packed columns showed superior efficiency and similar values for the minimum reduced plate heights (2.56-2.69) before correction for extra-column contribution compared to normal-bore columns. Moreover, the influence of extra-column contribution was investigated to demonstrate the difference between apparent and intrinsic efficiency by replacing the column by a zero dead volume union to determine the band spreading caused by the system. It was demonstrated that 72% of the intrinsic efficiency could be reached. The results of the kinetic plot analysis indicate the superior performance of the sub-2 μm fully porous particle packed column for ultra-fast liquid chromatography.
authors.affiliation: Institut für Energie- und Umwelttechnik e. V, IUTA (Institute of Energy and Environmental Technology), Bliersheimer Straße 58-60, Duisburg, Germany.|Instrumental Analytical Chemistry, University of Duisburg-Essen, Universitätsstr. 5, Essen, Germany.
authors.forename: Terence
authors.initials: T
authors.lastname: Hetzel
authors.affiliation: Institut für Energie- und Umwelttechnik e. V, IUTA (Institute of Energy and Environmental Technology), Bliersheimer Straße 58-60, Duisburg, Germany.
authors.forename: Denise
authors.initials: D
authors.lastname: Loeker
authors.affiliation: Institut für Energie- und Umwelttechnik e. V, IUTA (Institute of Energy and Environmental Technology), Bliersheimer Straße 58-60, Duisburg, Germany. teutenberg@iuta.de.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Teutenberg
authors.affiliation: Instrumental Analytical Chemistry, University of Duisburg-Essen, Universitätsstr. 5, Essen, Germany.
authors.forename: Torsten C
authors.initials: TC
authors.lastname: Schmidt
keywords: Core-shell particles; Fully-porous particles; Micro-bore columns; Miniaturization; Monolithic columns