title: Biophysical characterization of a model antibody drug conjugate.
abstract: Antibody drug conjugates (ADC) are important next-generation biopharmaceuticals and thus require stringent structure characterization as is the case for monoclonal antibodies. We have tested several biophysical techniques, i.e., circular dichroism, analytical ultracentrifugation, differential scanning calorimetry and fluorescence spectroscopy, to characterize a fluorescein-labeled monoclonal antibody as a model ADC. These techniques indicated possible small structure and stability changes by the conjugation, while largely retaining the tertiary structure of the antibody, consistent with unaltered biological activities. Thus, the above biophysical techniques are effective at detecting changes in the structural properties of ADC.
authors.affiliation: Alliance Protein Laboratories.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Arakawa
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kurosawa
authors.forename: Michael
authors.initials: M
authors.lastname: Storms
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Maruyama
authors.forename: C J
authors.initials: CJ
authors.lastname: Okumura
authors.forename: Nasib Karl
authors.initials: NK
authors.lastname: Maluf
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D055592:Biophysical Phenomena; D016466:CHO Cells; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D003412:Cricetulus; D016503:Drug Delivery Systems; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D018796:Immunoconjugates; D007074:Immunoglobulin G; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D014461:Ultracentrifugation
chemical_list: D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D018796:Immunoconjugates; D007074:Immunoglobulin G; D016650:Fluorescein-5-isothiocyanate