title: [Role of miR137-MITF in Prognostic Analysis of Multiple Myeloma].
abstract: Objectiive：To explore the effect of miR137 target gene MITF on the prognosis of multiple myeloma (MM).
  
  
  METHODS
  The target genes of miR137 were predicted by software, the GFP analysis was carried out for detecting MITF as the prognosis of multiple myeloma. The cell line overexpressing miR137 in MM cell line was constructed. Real-time qPCR and Western blot were used to detect the expression of MITF in this cell line.
  
  
  RESULTS
  The target genes of miR137 were MITF, BUE2H, SH3BP5 and KLF12. High expression of MITF in MM patients showed a good prognosis according to GFP analysis, but no significant difference was detected between the different subgroups. MITF expression was higher in MM cell line that over expressed miR137.
  
  
  CONCLUSIONS
  The miR137-MITF is an important index in judging the prognosis of multiple myeloma.
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China.
authors.forename: Shuai-Shuai
authors.initials: SS
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China.
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China.
authors.forename: Chang-Hong
authors.initials: CH
authors.lastname: Li
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Experimental Hematology,Institute of Hematology and Blood Disease Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300020,China. E-mail：drqiu99@medmail.com.cn.
authors.forename: Lu-Gui
authors.initials: LG
authors.lastname: Qiu
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D035683:MicroRNAs; D051739:Microphthalmia-Associated Transcription Factor; D009101:Multiple Myeloma; D011379:Prognosis
chemical_list: C497234:MITF protein, human; D035683:MicroRNAs; D051739:Microphthalmia-Associated Transcription Factor