title: Siglec-F is a novel intestinal M cell marker.
abstract: Intestinal microfold (M) cells are epithelial cells primarily present on Peyer's patches (PPs) in the small intestine. The ability of M cells to shuttle antigens into the PP for appropriate immune responses makes M cells a target for next-generation oral vaccine delivery. In this regard, discovery of M cell-specific receptors are of great interest, which could act as molecular tags for targeted delivery of cargo to M cells. Here, using a monoclonal antibody we generated to the Sialic acid-binding immunoglobulin-like lectin F (Siglec-F), we show that Siglec-F is expressed on mouse M cells in the small intestine. Immunohistochemical analysis of the PP tissue sections shows that Siglec-F is expressed on the surface of the M cell membrane exposed to the intestinal lumen. Anti-Siglec-F antibody injected into the mouse small intestine bound to M cells, demonstrating the potential to target M cells via Siglec-F.
authors.affiliation: Food and Health Institute Strategic Program, Institute of Food Research, Norwich, UK.
authors.forename: Nadezhda
authors.initials: N
authors.lastname: Gicheva
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Macauley
authors.affiliation: Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA.
authors.forename: Britni M
authors.initials: BM
authors.lastname: Arlian
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA; Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA; Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA, USA. Electronic address: jpaulson@scripps.edu.
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
authors.affiliation: Food and Health Institute Strategic Program, Institute of Food Research, Norwich, UK; Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA. Electronic address: norihito.kawasaki@ifr.ac.uk.
authors.forename: Norihito
authors.initials: N
authors.lastname: Kawasaki
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000915:Antibody Affinity / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D016466:CHO Cells; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding / Q000276:immunology; D011917:Rats, Inbred Lew; D063265:Sialic Acid Binding Immunoglobulin-like Lectins
chemical_list: D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; C443130:Siglecf protein, mouse
keywords: M cell; Oral vaccine; Peyer's patch; Siglec