title: Gametocidal System for Dissecting Wheat Chromosomes.
abstract: Gametocidal (Gc) system is a novel method of inducing chromosome mutations in wheat by using Gc genes of wheat-related species. Many deletion lines were efficiently produced by this system and used for physical mapping of genes and DNA markers. A large stocks with chromosome deletions in homozygous state as well as the lines with Gc genes are available in the gene bank of Japan (KOMUGI). In this chapter, I describe the method of inducing breakage in a target chromosome using lines with the Gc gene on chromosome 2C of Aegilops cylindrica (a wheat-related wild species) and nullisomic-tetrasomics.
authors.affiliation: Arid Land Research Center, Tottori University, 1390, Hamasaka, Tottori, 680-0001, Japan. tsujim@alrc.tottori-u.ac.jp.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D002869:Chromosome Aberrations; D019457:Chromosome Breakage; D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D057898:Hemizygote; D014908:Triticum / Q000235:genetics*
keywords: Addition line; Aegilops cylindrica; Chromosome dissection; Deletion stock; Gametocidal chromosome; KOMUGI; Nullisomic–tetrasomics