title: Effects of Wolbachia on mitochondrial DNA variation in populations of Athetis lepigone (Lepidoptera: Noctuidae) in China.
abstract: Wolbachia are endosymbiotic bacteria that infect arthropods and incompatibility among strains can affect gene flow within host insect populations that can significantly result in host mitochondrial DNA (MtDNA) variation within or among populations. The effects of Wolbachia infection on Athetis lepigone MtDNA variation were studied across 12 geographical populations sampled from North China. Mitochondrial COI gene and Wolbachia wsp gene were both obtained from 423 individuals, including 46 Wolbachia infected and 377 uninfected individuals. Average A. lepigone infection rate was 11% (range: 0 to 26%), and wsp sequence variation defined three distinct Wolbachia strains; wLep1, wLep2, and wLep3, respectively. A haplotype network showed no relationship between A. lepigone MtDNA haplotype and Wolbachia infection. Furthermore, haplotype variation mainly exists within populations, although the proportion of variation is higher within uninfected compared to infected individuals. The lack of correlation between A. lepigone haplotype and Wolbachia strain, suggests symbiont infection may not affect mating compatibility or gene flow within host populations in China.
authors.affiliation: a State Key Laboratory for Biology of Plant Diseases and Insect Pests , MOA - CABI Joint Laboratory for Bio-Safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing , P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.affiliation: b United States Department of Agriculture , Agricultural Research Service, Corn Insects & Crop Genetics Research Unit, Iowa State University , Ames, IA , USA.
authors.forename: Brad
authors.initials: B
authors.lastname: Coates
authors.affiliation: a State Key Laboratory for Biology of Plant Diseases and Insect Pests , MOA - CABI Joint Laboratory for Bio-Safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing , P.R. China.
authors.forename: Kang-Lai
authors.initials: KL
authors.lastname: He
authors.affiliation: a State Key Laboratory for Biology of Plant Diseases and Insect Pests , MOA - CABI Joint Laboratory for Bio-Safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing , P.R. China.
authors.forename: Shu-Xiong
authors.initials: SX
authors.lastname: Bai
authors.affiliation: a State Key Laboratory for Biology of Plant Diseases and Insect Pests , MOA - CABI Joint Laboratory for Bio-Safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing , P.R. China.
authors.forename: Tian-Tao
authors.initials: TT
authors.lastname: Zhang
authors.affiliation: a State Key Laboratory for Biology of Plant Diseases and Insect Pests , MOA - CABI Joint Laboratory for Bio-Safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing , P.R. China.
authors.forename: Zhen-Ying
authors.initials: ZY
authors.lastname: Wang
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000235:genetics*; D002681:China; D014644:Genetic Variation; D049750:Genome, Insect; D054629:Genome, Mitochondrial; D006239:Haplotypes; D009036:Moths / Q000235:genetics* / Q000382:microbiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D020577:Wolbachia
keywords: Athetis lepigone; MtDNA variation; Wolbachia; geographic populations