title: Draft genome sequence of subterranean clover, a reference for genus Trifolium.
abstract: Clovers (genus Trifolium) are widely cultivated across the world as forage legumes and make a large contribution to livestock feed production and soil improvement. Subterranean clover (T. subterraneum L.) is well suited for genomic and genetic studies as a reference species in the Trifolium genus, because it is an annual with a simple genome structure (autogamous and diploid), unlike the other economically important perennial forage clovers, red clover (T. pratense) and white clover (T. repens). This report represents the first draft genome sequence of subterranean clover. The 471.8 Mb assembled sequence covers 85.4% of the subterranean clover genome and contains 42,706 genes. Eight pseudomolecules of 401.1 Mb in length were constructed, based on a linkage map consisting of 35,341 SNPs. The comparative genomic analysis revealed that different clover chromosomes showed different degrees of conservation with other Papilionoideae species. These results provide a reference for genetic and genomic analyses in the genus Trifolium and new insights into evolutionary divergence in Papilionoideae species.
authors.affiliation: Kazusa DNA Research Institute, Kazusa-Kamatari 2-6-7, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Hirakawa
authors.affiliation: Centre for Plant Genetics and Breeding, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
authors.forename: Parwinder
authors.initials: P
authors.lastname: Kaur
authors.affiliation: Kazusa DNA Research Institute, Kazusa-Kamatari 2-6-7, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Shirasawa
authors.affiliation: Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, WA 6151, Australia.|School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
authors.forename: Phillip
authors.initials: P
authors.lastname: Nichols
authors.affiliation: Kazusa DNA Research Institute, Kazusa-Kamatari 2-6-7, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Nagano
authors.affiliation: Murdoch University, 90 South Street, Murdoch, WA 6150, Australia.
authors.forename: Rudi
authors.initials: R
authors.lastname: Appels
authors.affiliation: Centre for Plant Genetics and Breeding, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
authors.forename: William
authors.initials: W
authors.lastname: Erskine
authors.affiliation: Kazusa DNA Research Institute, Kazusa-Kamatari 2-6-7, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Sachiko N
authors.initials: SN
authors.lastname: Isobe
mesh_terms: D002874:Chromosome Mapping; D019295:Computational Biology; D017343:Genes, Plant; D008040:Genetic Linkage; D018745:Genome, Plant; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D029921:Trifolium / Q000235:genetics*