title: Suppression of Lipid Accumulation by Indole-3-Carbinol Is Associated with Increased Expression of the Aryl Hydrocarbon Receptor and CYP1B1 Proteins in Adipocytes and with Decreased Adipocyte-Stimulated Endothelial Tube Formation.
abstract: This study investigated the effects of indole-3-carbinol (I3C) on adipogenesis- and angiogenesis-associated factors in mature adipocytes. The cross-talk between mature adipocytes and endothelial cells (ECs) was also explored by cultivating ECs in a conditioned medium (CM) by using I3C-treated adipocytes. The results revealed that I3C significantly inhibited triglyceride accumulation in mature adipocytes in association with significantly increased expression of AhR and CYP1B1 proteins as well as slightly decreased nuclear factor erythroid-derived factor 2-related factor 2, hormone-sensitive lipase, and glycerol-3-phosphate dehydrogenase expression by mature adipocytes. Furthermore, I3C inhibited CM-stimulated endothelial tube formation, which was accompanied by the modulated secretion of angiogenic factors in adipocytes, including vascular endothelial growth factor, interleukin-6, matrix metalloproteinases, and nitric oxide. In conclusion, I3C reduced lipid droplet accumulation in adipocytes and suppressed adipocyte-stimulated angiogenesis in ECs, suggesting that I3C is a potential therapeutic agent for treating obesity and obesity-associated disorders.
authors.affiliation: School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan. b8706083@tmu.edu.tw.
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Wang
authors.affiliation: School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan. lin5611@tmu.edu.tw.
authors.forename: Shyh-Hsiang
authors.initials: SH
authors.lastname: Lin
authors.affiliation: Department of Food and Beverage Management, Mackay Medicine, Nursing and Management College, Taipei 112, Taiwan. s212@eip.mkc.edu.tw.
authors.forename: Yuan-Yu
authors.initials: YY
authors.lastname: Hou
authors.affiliation: School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan. yuehwa@tmu.edu.tw.|Cancer Research Center, Taipei Medical University Hospital, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan. yuehwa@tmu.edu.tw.
authors.forename: Yue-Hwa
authors.initials: YH
authors.lastname: Chen
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D042783:Endothelial Cells / Q000502:physiology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics* / Q000378:metabolism
chemical_list: D019440:Anti-Obesity Agents; D007211:Indoles; D018336:Receptors, Aryl Hydrocarbon; C016517:indole-3-carbinol
keywords: adipocytes; adipogenesis; angiogenesis; aryl hydrocarbon receptor; indole-3-carbinol; obesity