title: Single dish gradient screening of small molecule localization.
abstract: Understanding trafficking in cells and tissues is one of the most critical steps in exploring the mechanisms and modes of action (MOAs) of a small molecule. Typically, deciphering the role of concentration presents one of the most difficult challenges associated with this task. Herein, we present a practical solution to this problem by developing concentration gradients within single dishes of cells. We demonstrate the method by evaluating fluorescently-labelled probes developed from two classes of natural products that have been identified as potential anti-cancer leads by STORM super-resolution microscopy.
authors.affiliation: The Salk Institute for Biological Sciences, 10010 North Torrey Pines Rd, La Jolla, CA 92037, USA. hucang@salk.edu.
authors.forename: Paolo
authors.initials: P
authors.lastname: Beuzer
authors.forename: Joshua
authors.initials: J
authors.lastname: Axelrod
authors.forename: Lynnie
authors.initials: L
authors.lastname: Trzoss
authors.forename: Willam
authors.initials: W
authors.lastname: Fenical
authors.forename: Ramesh
authors.initials: R
authors.lastname: Dasari
authors.forename: Antonio
authors.initials: A
authors.lastname: Evidente
authors.forename: Alexander
authors.initials: A
authors.lastname: Kornienko
authors.forename: Hu
authors.initials: H
authors.lastname: Cang
authors.forename: James J
authors.initials: JJ
authors.lastname: La Clair