title: Circulating Erythrocyte Microparticles and the Biochemical Extent of Myocardial Injury in ST Elevation Myocardial Infarction.
abstract: OBJECTIVE
  Red blood cell microparticles (RBCm) have potential adverse vascular effects and they have been shown to be elevated in ST elevation myocardial infarction (STEMI). The purpose of this study is to investigate their relationship with biochemical infarct size.
  
  
  METHODS
  RBCm were quantified with flow cytometry in blood drawn from 60 STEMI patients after a primary angioplasty. The creatine kinase-myocardial brain fraction (CK-MB) was measured at predefined time points and the area under the curve (AUC) was calculated.
  
  
  RESULTS
  RBCm count was correlated with CK-MB AUC (Spearman's ρ = 0.83, p < 0.001). The CK-MB AUC values per RBCm quartile (lower to upper) were: 3,351 (2,452-3,608), 5,005 (4,450-5,424), 5,903 (4,862-10,594), and 8,406 (6,848-12,782) ng × h/ml, respectively. From lower to upper quartiles, the maximal troponin I values were: 42.2 (23.3-49.3), 49.6 (28.8-54.1), 59.2 (41.4-77.3), and 69.1 (48.0-77.5) ng/ml (p = 0.005). In multivariable analysis, RBCm remained a significant predictor of CK-MB AUC (standardized β = 0.63, adjusted p = 0.001).
  
  
  CONCLUSIONS
  Erythrocyte microparticles appear to be related to the total myocardial damage biomarker output. The exact pathophysiologic routes, if any, for this interaction remain to be identified. However, these results suggest that erythrocytes may be a - thus far virtually ignored - player in the pathogenesis of ischemic injury.
authors.affiliation: 2nd Department of Cardiology, Attikon University Hospital, National and Kapodistrean University of Athens Medical School, Athens, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Giannopoulos
authors.forename: Dimitrios A
authors.initials: DA
authors.lastname: Vrachatis
authors.forename: Georgios
authors.initials: G
authors.lastname: Oudatzis
authors.forename: Georgios
authors.initials: G
authors.lastname: Paterakis
authors.forename: Christos
authors.initials: C
authors.lastname: Angelidis
authors.forename: Athanasios
authors.initials: A
authors.lastname: Koutivas
authors.forename: Georgios
authors.initials: G
authors.lastname: Sianos
authors.forename: Michael W
authors.initials: MW
authors.lastname: Cleman
authors.forename: Gerasimos
authors.initials: G
authors.lastname: Filippatos
authors.forename: John
authors.initials: J
authors.lastname: Lekakis
authors.forename: Spyridon
authors.initials: S
authors.lastname: Deftereos
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D055252:Cell-Derived Microparticles; D052279:Creatine Kinase, MB Form / Q000097:blood*; D004906:Erythrocyte Count; D004912:Erythrocytes; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009206:Myocardium / Q000473:pathology*; D009336:Necrosis; D000072657:ST Elevation Myocardial Infarction / Q000097:blood* / Q000473:pathology; D019210:Troponin I / Q000097:blood*
chemical_list: D015415:Biomarkers; D019210:Troponin I; D052279:Creatine Kinase, MB Form