title: Mechanism Underlying the Onset of Internal Blue Discoloration in Japanese Radish (Raphanus sativus) Roots.
abstract: The internal blue discoloration observed in Japanese radish (Raphanus sativus L.) roots is a physiological phenomenon caused by storage following harvest at approximately 20 °C and poses a serious problem for farmers. Here, we describe the mechanism underlying the onset of internal blue discoloration of three cultivars: Hukuhomare, SC8-260, and Yuto. Each cultivar was maintained under the same conditions. Additionally, Hukuhomare radish roots were maintained at three different cultivation conditions in a related experiment. The blue discoloration in radish roots was caused by the oxidation of 4-hydroxyglucobrassicin as a result of an increase in oxidative stress involving peroxidase. Thus, the extent of blue discoloration was influenced by the chemical balance involving 4-hydroxyglucobrassicin content, antioxidant capacity, and oxidation activity.
authors.affiliation: Graduate School of Bioresources, Mie University , 1577 Kurimamachiya, Tsu, Mie 514-8507, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Teranishi
authors.affiliation: Food Research Institute, National Agriculture and Food Research Organization , 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Nagata
authors.initials: N
authors.lastname: Masayasu
authors.affiliation: Sand Dune Agricultural Research Center, Agricultural Experiment Station, Ishikawa Agriculture and Forestry Research Center , I 5-2 Uchihisumi, Kahoku, Ishikawa 929-1126, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Masuda
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D003116:Color; D005961:Glucosinolates / Q000378:metabolism; D007211:Indoles / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D018517:Plant Roots / Q000737:chemistry* / Q000378:metabolism; D031224:Raphanus / Q000737:chemistry* / Q000378:metabolism
chemical_list: C428965:4-hydroxyindol-3-ylmethylglucosinolate; D000975:Antioxidants; D005961:Glucosinolates; D007211:Indoles
keywords: 4-hydroxyglucobrassicin; Japanese radish; Raphanus sativus; blue discoloration; roots