title: Antimicrobial silver-filled silica nanorattles with low immunotoxicity in dendritic cells.
abstract: The progression in the use of orthopedic implants has led to an increase in the absolute number of implant infections, triggering a search for more effective antibacterial coatings. Nanorattles have recently gained interest in biomedical applications such as drug delivery, as encapsulation of the cargo inside the hollow structure provides a physical protection from the surrounding environment. Here, silver-containing silica nanorattles (Ag@SiO2) were evaluated for their antimicrobial potential and for their impact on cells of the immune system. We show that Ag@SiO2nanorattles exhibited a clear antibacterial effect against Escherichia coli as well as Staphylococcus aureus found in post-operative infections. Immunotoxicological analyses showed that the particles were taken up through an active phagocytic process by dendritic cells of the immune system and did not affect their viability nor induce unwanted immunological effects. Silver-containing silica nanorattles thus fulfill several prerequisites for an antibacterial coating on surgical implants.
authors.affiliation: Department of Chemistry, University of Fribourg, Fribourg, Switzerland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Priebe
authors.affiliation: Department of Medicine, University of Fribourg, Fribourg, Switzerland.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Widmer
authors.affiliation: Department of Medicine, University of Fribourg, Fribourg, Switzerland.
authors.forename: Nina
authors.initials: N
authors.lastname: Suhartha Löwa
authors.affiliation: Department of Chemistry, University of Fribourg, Fribourg, Switzerland.
authors.forename: Sarah-Luise
authors.initials: SL
authors.lastname: Abram
authors.affiliation: Department of Medicine, University of Fribourg, Fribourg, Switzerland.
authors.forename: Inès
authors.initials: I
authors.lastname: Mottas
authors.affiliation: Department of Biomedicine, University Hospital Basel, Basel, Switzerland.
authors.forename: Anne-Kathrin
authors.initials: AK
authors.lastname: Woischnig
authors.affiliation: Department of Chemistry, University of Fribourg, Fribourg, Switzerland.
authors.forename: Priscilla S
authors.initials: PS
authors.lastname: Brunetto
authors.affiliation: Department of Biomedicine, University Hospital Basel, Basel, Switzerland.
authors.forename: Nina
authors.initials: N
authors.lastname: Khanna
authors.affiliation: Department of Medicine, University of Fribourg, Fribourg, Switzerland. Electronic address: carole.bourquin@unifr.ch.
authors.forename: Carole
authors.initials: C
authors.lastname: Bourquin
authors.affiliation: Department of Chemistry, University of Fribourg, Fribourg, Switzerland. Electronic address: katharina.fromm@unifr.ch.
authors.forename: Katharina M
authors.initials: KM
authors.lastname: Fromm
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008826:Microbial Sensitivity Tests; D010587:Phagocytosis; D012822:Silicon Dioxide / Q000737:chemistry*; D012834:Silver / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver; D012822:Silicon Dioxide
keywords: Antimicrobial properties; Dendritic cells; Immunoresponse; Nanorattles; Silica nanocontainers; Silver nanoparticles