title: A Multimodal Imaging Approach for Longitudinal Evaluation of Bladder Tumor Development in an Orthotopic Murine Model.
abstract: Bladder cancer is the fourth most common malignancy amongst men in Western industrialized countries with an initial response rate of 70% for the non-muscle invasive type, and improving therapy efficacy is highly needed. For this, an appropriate, reliable animal model is essential to gain insight into mechanisms of tumor growth for use in response monitoring of (new) agents. Several animal models have been described in previous studies, but so far success has been hampered due to the absence of imaging methods to follow tumor growth non-invasively over time. Recent developments of multimodal imaging methods for use in animal research have substantially strengthened these options of in vivo visualization of tumor growth. In the present study, a multimodal imaging approach was addressed to investigate bladder tumor proliferation longitudinally. The complementary abilities of Bioluminescence, High Resolution Ultrasound and Photo-acoustic Imaging permit a better understanding of bladder tumor development. Hybrid imaging modalities allow the integration of individual strengths to enable sensitive and improved quantification and understanding of tumor biology, and ultimately, can aid in the discovery and development of new therapeutics.
authors.affiliation: Department of Radiology & Nuclear Medicine, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Chantal
authors.identifier: http://orcid.org/0000-0003-4243-0095
authors.initials: C
authors.lastname: Scheepbouwer
authors.affiliation: FUJIFILM VisualSonics Inc., Amsterdam, The Netherlands.
authors.forename: Sandra
authors.initials: S
authors.lastname: Meyer
authors.affiliation: Department of Radiology & Nuclear Medicine, VU University Medical Center, Amsterdam, The Netherlands.|Department of Medical Microbiology and Infection control, Amsterdam, The Netherlands.
authors.forename: Maroeska J
authors.initials: MJ
authors.lastname: Burggraaf
authors.affiliation: FUJIFILM VisualSonics Inc., Amsterdam, The Netherlands.
authors.forename: Jithin
authors.initials: J
authors.lastname: Jose
authors.affiliation: Department of Radiology & Nuclear Medicine, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Carla F M
authors.initials: CF
authors.lastname: Molthoff
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D008137:Longitudinal Studies; D051379:Mice; D008810:Mice, Inbred C57BL; D064847:Multimodal Imaging / Q000379:methods*; D009163:Mycobacterium bovis / Q000276:immunology; D009368:Neoplasm Transplantation; D061088:Photoacoustic Techniques / Q000379:methods*; D014463:Ultrasonography / Q000379:methods*; D001749:Urinary Bladder Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology