title: Brain-derived Neurotrophic Factor Promotes the Migration of Olfactory Ensheathing Cells Through TRPC Channels.
abstract: Olfactory ensheathing cells (OECs) are a unique type of glial cells with axonal growth-promoting properties in the olfactory system. Organized migration of OECs is essential for neural regeneration and olfactory development. However, the molecular mechanism of OEC migration remains unclear. In the present study, we examined the effects of brain-derived neurotrophic factor (BDNF) on OEC migration. Initially, the "scratch" migration assay, the inverted coverslip and Boyden chamber migration assays showed that BDNF could promote the migration of primary cultured OECs. Furthermore, BDNF gradient attracted the migration of OECs in single-cell migration assays. Mechanistically, TrkB receptor expressed in OECs mediated BDNF-induced OEC migration, and BDNF triggered calcium signals in OECs. Finally, transient receptor potential cation channels (TRPCs) highly expressed in OECs were responsible for BDNF-induced calcium signals, and required for BDNF-induced OEC migration. Taken together, these results demonstrate that BDNF promotes the migration of cultured OECs and an unexpected finding is that TRPCs are required for BDNF-induced OEC migration. GLIA 2016;64:2154-2165.
authors.affiliation: Key Laboratory of Arrhythmias, Ministry of Education, East Hospital, and Department of Anatomy and Neurobiology, Collaborative Innovation Center for Brain Science, Tongji University School of Medicine, Shanghai, 200092, China.|Institute of Neuroscience and Institute of Hypoxia Medicine, Department of Basic Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Spine Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Teng
authors.affiliation: Institute of Neuroscience and Institute of Hypoxia Medicine, Department of Basic Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Institute of Neuroscience and Institute of Hypoxia Medicine, Department of Basic Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Arrhythmias, Ministry of Education, East Hospital, and Department of Anatomy and Neurobiology, Collaborative Innovation Center for Brain Science, Tongji University School of Medicine, Shanghai, 200092, China. dingyuqiang@vip.163.com.|Institute of Neuroscience and Institute of Hypoxia Medicine, Department of Basic Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. dingyuqiang@vip.163.com.
authors.forename: Yu-Qiang
authors.initials: YQ
authors.lastname: Ding
authors.affiliation: Institute of Neuroscience and Institute of Hypoxia Medicine, Department of Basic Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. hzhzju021@163.com.
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Huang
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002227:Carbazoles / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D026121:Indole Alkaloids / Q000494:pharmacology; D009419:Nerve Tissue Proteins; D009457:Neuroglia / Q000187:drug effects*; D009830:Olfactory Bulb / Q000166:cytology*; D011804:Quinolines / Q000378:metabolism; D034622:RNA Interference / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D050052:TRPC Cation Channels / Q000235:genetics / Q000378:metabolism; D000074287:TRPC6 Cation Channel / Q000235:genetics / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D002227:Carbazoles; D004791:Enzyme Inhibitors; D026121:Indole Alkaloids; D009419:Nerve Tissue Proteins; D011804:Quinolines; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; D009418:S100 Proteins; C495966:TOTO-3 compound; D050052:TRPC Cation Channels; C104603:TRPC3 cation channel; D000074287:TRPC6 Cation Channel; D013844:Thiazoles; C068313:Ngfr protein, rat; C049985:staurosporine aglycone; D002118:Calcium
keywords: BDNF; TRPCs; calcium; migration; olfactory ensheathing cells