title: Protein phosphatase 2A regulatory subunit B56β modulates erythroid differentiation.
abstract: Anemia due to attenuated erythroid terminal differentiation is one of the most common hematological disorders occurring at all stages of life. We previously demonstrated that catalytic subunit α of protein phosphatase 2A (PP2Acα) modulates fetal liver erythropoiesis. However the corresponding PP2A regulatory subunit in this process remains unknown. In this study, we report that chemical inhibition of PP2A activity with okadaic acid impairs hemin-induced erythroid differentiation. Interestingly, B56 family member B56β is the only regulatory subunit whose expression is induced by both erythropoietin in fetal liver cells and hemin in erythroleukemia K562 cells. Finally, knockdown of B56β attenuates hemin-induced K562 erythroid differentiation. Collectively, our data identify B56β as the potential functional regulatory subunit of PP2A in erythroid differentiation, shedding light on new target for precise modulation of PP2A activity for treatment of anemia and related diseases.
authors.affiliation: Orthopedic Department of the Second Affiliated Hospital of Soochow University, Suzhou 215000, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Wu
authors.affiliation: Emergency Department of the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cardiology of the First Affiliated Hospital, Soochow University, Suzhou 215006, China.
authors.forename: Xiansheng
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou 215006, China.
authors.forename: Yueqiu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou 215006, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
authors.affiliation: Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou 215006, China.
authors.forename: Shizhen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Jiangsu Province Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210000, China. Electronic address: huangzan@njau.edu.cn.
authors.forename: Zan
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Institute for Cardiovascular Science & Department of Cardiovascular Surgery of the First Affiliated Hospital, Soochow University, Suzhou 215006, China. Electronic address: chenweiqian@suda.edu.cn.
authors.forename: Weiqian
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215006, China. Electronic address: uuzyshen@126.com.
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Shen
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D041905:Erythroid Cells / Q000166:cytology* / Q000201:enzymology*; D055785:Gene Knockdown Techniques; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D020014:K562 Cells; D008565:Membrane Proteins / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D019319:Okadaic Acid / Q000494:pharmacology; D054648:Protein Phosphatase 2 / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D019319:Okadaic Acid; D006427:Hemin; C503493:PPP2R5B protein, human; D054648:Protein Phosphatase 2
keywords: B56β; Erythroid differentiation; Erythropoiesis; PPP2R5B; Protein phosphatase 2A; Regulatory subunits