title: Prokaryote community dynamics in anaerobic co-digestion of swine manure, rice straw and industrial clay residuals.
abstract: The aim of this study was to analyze the effect of the addition of rice straw and clay residuals on the prokaryote methane-producing community structure in a semi-continuously stirred tank reactor fed with swine manure. Molecular techniques, including terminal restriction fragment length polymorphism and a comparative nucleotide sequence analyses of the prokaryotic 16S rRNA genes, were performed. The results showed a positive effect of clay addition on methane yield during the co-digestion of swine manure and rice straw. At the digestion of swine manure, the bacterial phylum Firmicutes and the archaeal family Methanosarcinaceae, particularly Methanosarcina species, were predominant. During the co-digestion of swine manure and rice straw the microbial community changed, and with the addition of clay residual, the phylum Bacteroidetes predominated. The new nutritional conditions resulted in a shift in the archaeal family Methanosarcinaceae community as acetoclastic Methanosaeta species became dominant.
authors.affiliation: Universidad de Sancti Spíritus, Ave. de los Mártires, No. 360, Sancti Spíritus, Sancti Spíritus CP 60100, Cuba E-mail: janet@uniss.edu.cu.
authors.forename: Janet
authors.initials: J
authors.lastname: Jiménez
authors.affiliation: Department Bioengineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Max-Eyth-Allee 100, Potsdam D-14469, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Theuerl
authors.affiliation: Department Bioengineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Max-Eyth-Allee 100, Potsdam D-14469, Germany.
authors.forename: Ingo
authors.initials: I
authors.lastname: Bergmann
authors.affiliation: Department Bioengineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Max-Eyth-Allee 100, Potsdam D-14469, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Klocke
authors.affiliation: Facultad de Biología. Universidad de La Habana, Calle 25 e/ I y J, Vedado, La Habana CP. 10400, Cuba.
authors.forename: Gilda
authors.initials: G
authors.lastname: Guerra
authors.affiliation: Universidad de Sancti Spíritus, Ave. de los Mártires, No. 360, Sancti Spíritus, Sancti Spíritus CP 60100, Cuba E-mail: janet@uniss.edu.cu.
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Romero-Romero
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry*; D000693:Anaerobiosis; D000818:Animals; D001105:Archaea / Q000145:classification / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D019149:Bioreactors / Q000382:microbiology*; D000077215:Clay; D008372:Manure / Q000382:microbiology*; D008697:Methane; D012275:Oryza / Q000737:chemistry*; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013552:Swine / Q000382:microbiology*
chemical_list: D000538:Aluminum Silicates; D008372:Manure; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D008697:Methane; D000077215:Clay