title: The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice.
abstract: Background. Elastase mediates important oxidative actions during the development of chronic obstructive pulmonary disease (COPD). However, few resources for the inhibition of elastase have been investigated. Our study evaluated the ability of the recombinant plant derived Bauhinia bauhinioides Kallikrein proteinase Inhibitor (rBbKI) to modulate elastase-induced pulmonary inflammation. Methods. C57Bl/6 mice were given intratracheal elastase (ELA group) or saline (SAL group) and were treated intraperitoneally with rBbKI (ELA-rBbKI and SAL-rBbKI groups). At day 28, the following analyses were performed: (I) lung mechanics, (II) exhaled nitric oxide (ENO), (III) bronchoalveolar lavage fluid (BALF), and (IV) lung immunohistochemical staining. Results. In addition to decreasing mechanical alterations and alveolar septum disruption, rBbKI reduced the number of cells in the BALF and decreased the cellular expression of TNF-α, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS in airways and alveolar walls compared with the ELA group. rBbKI decreased the volume proportion of 8-iso-PGF2α, collagen, and elastic fibers in the airways and alveolar walls compared with the ELA group. A reduction in the number of MUC-5-positive cells in the airway walls was also observed. Conclusion. rBbKI reduced elastase-induced pulmonary inflammation and extracellular matrix remodeling. rBbKI may be a potential pharmacological tool for COPD treatment.
authors.affiliation: Department of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, Brazil.
authors.forename: Bruno Tadeu
authors.initials: BT
authors.lastname: Martins-Olivera
authors.affiliation: Department of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, Brazil.
authors.forename: Rafael
authors.identifier: 0000-0002-9270-8123
authors.initials: R
authors.lastname: Almeida-Reis
authors.affiliation: Department of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, Brazil.
authors.forename: Osmar Aparecido
authors.initials: OA
authors.lastname: Theodoro-Júnior
authors.affiliation: Department of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, Brazil.
authors.forename: Leandro Vilela
authors.initials: LV
authors.lastname: Oliva
authors.affiliation: Department of Biochemistry, Universidade Federal de São Paulo, 04044-020 São Paulo, SP, Brazil.
authors.forename: Natalia
authors.initials: N
authors.lastname: Neto Dos Santos Nunes
authors.affiliation: Department of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, Brazil.
authors.forename: Clarice Rosa
authors.initials: CR
authors.lastname: Olivo
authors.affiliation: Department of Biochemistry, Universidade Federal de São Paulo, 04044-020 São Paulo, SP, Brazil.
authors.forename: Marlon
authors.initials: M
authors.lastname: Vilela de Brito
authors.affiliation: Department of Bioscience, Federal University of Sao Paulo, 11015-020 Santos, SP, Brazil.
authors.forename: Carla Máximo
authors.initials: CM
authors.lastname: Prado
authors.affiliation: Department of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, Brazil.
authors.forename: Edna Aparecida
authors.initials: EA
authors.lastname: Leick
authors.affiliation: Department of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, Brazil.
authors.forename: Mílton de Arruda
authors.initials: Mde A
authors.lastname: Martins
authors.affiliation: Department of Biochemistry, Universidade Federal de São Paulo, 04044-020 São Paulo, SP, Brazil.
authors.forename: Maria Luiza Vilela
authors.identifier: 0000-0002-5822-0060
authors.initials: ML
authors.lastname: Oliva
authors.affiliation: Department of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, Brazil.
authors.forename: Renato Fraga
authors.identifier: 0000-0001-6234-1458
authors.initials: RF
authors.lastname: Righetti
authors.affiliation: Department of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, Brazil.
authors.forename: Iolanda de Fátima Lopes Calvo
authors.identifier: 0000-0002-5662-7895
authors.initials: Ide F
authors.lastname: Tibério
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D004646:Emphysema / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010196:Pancreatic Elastase / Q000494:pharmacology*; D010940:Plant Proteins / Q000627:therapeutic use; D011480:Protease Inhibitors / Q000627:therapeutic use*
chemical_list: C434623:BbKI protein, Bauhinia bauhinioides; D010940:Plant Proteins; D011480:Protease Inhibitors; D010196:Pancreatic Elastase