title: Population structure analysis and association mapping of blast resistance in indica rice (Oryza sativa L.) landraces.
abstract: Rice blast caused by Magnaporthe oryzae is one of the most devastating rice diseases worldwide. To understand the genetic diversity of indica landrace accessions and identify simple sequence repeat (SSR) markers that are associated with blast resistance, a population of 276 indica landraces from across the world was constructed. This population was then used to evaluate the blast-resistance phenotype through artificial inoculation under controlled conditions in 2012 and 2013. The genetic diversity and association of the population with resistance were analyzed by examining the phenotype for 160 SSR markers distributed on 12 rice chromosomes. The 276 accessions were classified into seven groups using model- and distance-based cluster analyses. Associations between SSR markers and blast resistance showed that 26 SSR markers were significantly associated with blast resistance in 2012 and 2013 (P < 0.01) and that the phenotypic variation ranged from 2.68 to 13.11%. Nineteen of the markers associated with blast resistance were located in regions where genes or quantitative trait loci (QTLs) have been previously reported, and seven were newly identified in this study. These results indicate that marker-trait association has potential advantages over classical linkage analysis and QTL mapping, and that these markers could be used for marker-assisted selection in rice blast-resistance-breeding programs.
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.|Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.
authors.forename: J B
authors.initials: JB
authors.lastname: He
authors.affiliation: Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, China.
authors.forename: A H
authors.initials: AH
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.|Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, China.
authors.forename: N Y
authors.initials: NY
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.|Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, China.
authors.forename: W W
authors.initials: WW
authors.lastname: He
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.|Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Dang
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.|Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, China.
authors.forename: G Y
authors.initials: GY
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.|Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, China.
authors.forename: J
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China yongmeibao@njau.edu.cn.|Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, China yongmeibao@njau.edu.cn.
authors.forename: Y M
authors.initials: YM
authors.lastname: Bao
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.|Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, China.
authors.forename: H S
authors.initials: HS
authors.lastname: Zhang
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D016000:Cluster Analysis; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D056726:Genetic Association Studies; D008040:Genetic Linkage; D014644:Genetic Variation; D054884:Host-Pathogen Interactions; D020082:Magnaporthe / Q000502:physiology; D018895:Microsatellite Repeats; D012275:Oryza / Q000235:genetics* / Q000382:microbiology; D000069600:Plant Breeding; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D040641:Quantitative Trait Loci