title: Association of single nucleotide polymorphism rs2065955 of the filaggrin gene with susceptibility to Epstein-Barr virus-associated gastric carcinoma and EBV-negative gastric carcinoma.
abstract: The relationship between the Filaggrin gene (FLG) rs2065955 polymorphism and susceptibility to Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC) and EBV-negative gastric carcinoma (EBVnGC) was investigated in Shandong Province, China. We detected the FLG rs2065955 genotype and allele distribution by using PCR and restriction fragment length polymorphism (RFLP) in 64 EBVaGC, 82 EBVnGC, and 111 normal control samples. Immunohistochemistry was used to detect the level of FLG protein in 35 EBVaGC and 51 EBVnGC tumor tissues. Compared with normal controls, the genotype CC and allele C of FLG rs2065955 showed higher frequency in EBVaGC and EBVnGC. There was no significant difference between EBVaGC and EBVnGC in allele distribution of FLG rs2065955, but the genotype CC was found more frequently in EBVaGC than in EBVnGC. The risk of developing either EBVaGC or EBVnGC in genotype CC was higher than in other genotypes. Furthermore, genotype CC of FLG rs2065955 may contribute more to the risk of developing EBVaGC than EBVnGC. There was no significant difference in the expression level of FLG protein between EBVaGC and EBVnGC. In conclusion, the FLG rs2065955 polymorphism was significantly related to gastric carcinoma. Allele C of FLG rs2065955 could be a risk factor for EBVaGC or EBVnGC, while genotype CC of FLG rs2065955 was especially associated with EBVaGC.
authors.affiliation: Department of Medical Microbiology, Qingdao University Medical College, Qingdao, 266021, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Kuang
authors.affiliation: Department of Pathology, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Blood Transfusion, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Medical Microbiology, Qingdao University Medical College, Qingdao, 266021, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Medical Microbiology, Qingdao University Medical College, Qingdao, 266021, China.
authors.forename: Danrui
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Medical Microbiology, Qingdao University Medical College, Qingdao, 266021, China.
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Medical Microbiology, Qingdao University Medical College, Qingdao, 266021, China. qdluobing@163.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Luo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000235:genetics* / Q000821:virology; D020031:Epstein-Barr Virus Infections / Q000235:genetics* / Q000821:virology; D005260:Female; D000091344:Filaggrin Proteins; D020022:Genetic Predisposition to Disease; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000235:genetics* / Q000821:virology; D055815:Young Adult
chemical_list: C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins
keywords: Epstein-Barr virus (EBV); Filaggrin gene (FLG); gastric carcinoma; gene polymorphism; immunohistochemistry; rs2065955