title: Ultra-High Resolution Ion Mobility Separations Utilizing Traveling Waves in a 13 m Serpentine Path Length Structures for Lossless Ion Manipulations Module.
abstract: We report the development and initial evaluation of a 13 m path length Structures for Lossless Manipulations (SLIM) module for achieving high resolution separations using traveling waves (TW) with ion mobility (IM) spectrometry. The TW SLIM module was fabricated using two mirror-image printed circuit boards with appropriately configured RF, DC, and TW electrodes and positioned with a 2.75 mm intersurface gap. Ions were effectively confined in field-generated conduits between the surfaces by RF-generated pseudopotential fields and moved losslessly through a serpentine path including 44 "U" turns using TWs. The ion mobility resolution was characterized at different pressures, gaps between the SLIM surfaces, and TW and RF parameters. After initial optimization, the SLIM IM-MS module provided about 5-fold higher resolution separations than present commercially available drift tube or traveling wave IM-MS platforms. Peak capacity and peak generation rates achieved were 246 and 370 s(-1), respectively, at a TW speed of 148 m/s. The high resolution achieved in the TW SLIM IM-MS enabled, e.g., isomeric sugars (lacto-N-fucopentaose I and lacto-N-fucopentaose II) to be baseline resolved, and peptides from an albumin tryptic digest were much better resolved than with existing commercial IM-MS platforms. The present work also provides a foundation for the development of much higher resolution SLIM devices based upon both considerably longer path lengths and multipass designs.
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Liulin
authors.initials: L
authors.lastname: Deng
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Yehia M
authors.initials: YM
authors.lastname: Ibrahim
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Hamid
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Sandilya V B
authors.initials: SV
authors.lastname: Garimella
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Ian K
authors.initials: IK
authors.lastname: Webb
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Xueyun
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Spencer A
authors.initials: SA
authors.lastname: Prost
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Sandoval
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Randolph V
authors.initials: RV
authors.lastname: Norheim
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Gordon A
authors.initials: GA
authors.lastname: Anderson
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Aleksey V
authors.initials: AV
authors.lastname: Tolmachev
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Erin S
authors.initials: ES
authors.lastname: Baker
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory , 902 Battelle Blvd., P.O. Box 999, Richland, Washington 99352, United States.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
mesh_terms: D000818:Animals; D002417:Cattle; D004566:Electrodes; D055590:Electromagnetic Phenomena; D004867:Equipment Design; D007477:Ions / Q000032:analysis; D007536:Isomerism; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D010455:Peptides / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D007477:Ions; D009844:Oligosaccharides; D010455:Peptides; C041762:lacto-N-fucopentaose II; D012710:Serum Albumin, Bovine; C045442:lacto-N-fucopentaose I