title: Lactate Formation in Primary and Metastatic Colon Cancer Cells at Hypoxia and Normoxia.
abstract: High glucose consumption and lactate synthesis in aerobic glycolysis are a hallmark of cancer cells. They can form lactate also in glutaminolysis, but it is not clear how oxygen availability affects this process. We studied lactate synthesis at various oxygen levels in human primary (SW480) and metastatic (SW620) colon cancer cells cultured with L-Ser and/or L-Asp. Glucose and lactate levels were determined colorimetrically, amino acids by HPLC, expression of AST1-mRNA and AST2-mRNA by RT-PCR. In both lines glucose consumption and lactate synthesis were higher at 10% than at 1% oxygen, and lactate/glucose ratio was increased above 2.0 by L-Asp. AST1-mRNA expression was independent on oxygen and cell line, but AST2-mRNA was lower at hypoxia in SW480. We conclude that, in both cell lines at 1% hypoxia, lactate is formed mainly from glucose but at 10% normoxia also from L-Asp. At 10% normoxia, lactate synthesis is more pronounced in primary than metastatic colon cancer cells.
authors.affiliation: Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Graboń
authors.affiliation: Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Dagmara
authors.initials: D
authors.lastname: Otto-Ślusarczyk
authors.affiliation: Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Chrzanowska
authors.affiliation: Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Mielczarek-Puta
authors.affiliation: Department of Clinical and Experimental Pharmacology, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Ilona
authors.initials: I
authors.lastname: Joniec-Maciejak
authors.affiliation: Department of Clinical and Experimental Pharmacology, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Słabik
authors.affiliation: Chair and Department of Biochemistry, Medical University of Warsaw, Warsaw, Poland. akuzma@wum.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Barańczyk-Kuźma
mesh_terms: D001219:Aspartate Aminotransferases / Q000235:genetics / Q000378:metabolism; D001224:Aspartic Acid / Q000494:pharmacology; D002452:Cell Count; D015687:Cell Hypoxia / Q000187:drug effects; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D003470:Culture Media / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism*; D008954:Models, Biological; D009362:Neoplasm Metastasis; D010100:Oxygen / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012694:Serine / Q000494:pharmacology
chemical_list: D003470:Culture Media; D012333:RNA, Messenger; D001224:Aspartic Acid; D019344:Lactic Acid; D012694:Serine; D001219:Aspartate Aminotransferases; D005947:Glucose; D010100:Oxygen
keywords: L-aspartate; L-serine; aspartate aminotransferases; colon cancer cell lines; expression; hypoxia; lactate synthesis; tissue normoxia