title: Protective effect of Cistanchis A on ethanol-induced damage in primary cultured mouse hepatocytes.
abstract: Cistanoside A (C. A) was one of phenylethanol glycosides isolated from Cistanche deserticola, a tonic in traditional Chinese medicine. In our previous research, we demonstrated that Cistanoside A (C. A) possess the protective activities on CCl4induced hepatotoxicity in mice, such as increasing free radicals clearing activities, alleviating lipid-overoxidation damage, and improving respiratory chain function in mitochondria. Meanwhile, our previous research also demonstrated C.A possess protective activities on alcohol induced hepatotoxicity in mice, shown in ameliorate the hepatic function indices, lightening steatosis and inflammatory infiltration, increasing free radicals clearing activities, alleviating lipid-overoxidation damage, and alleviating energy metabolism in mitochondria. The aim of this research was to evaluate the effects of Cistanoside A (C. A) on ethanol-induced damage in primary cultured mouse hepatocytes, and probe into the mechanism related. Using fluorescent staining, flow cytometer, immunohistochemistry analysis, and Western blotting, we demonstrated that C.A could enhance the survival rate of the primary cultured hepatocytes, alleviate apoptosis and necrosis, the mechanism was involved with enhance the expression of apoptosis inhibition factor bcl-2, and inhibition the expression of immediate early genes c-fos.
authors.affiliation: Gansu University of Chinese Medicine, AV.Dingxi, 30, Lanzhou, 730000, PR China; Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicine of Gansu Province, AV. Dingxi, 30, Lanzhou, 730000, PR China. Electronic address: louria@126.com.
authors.forename: Huiying
authors.initials: H
authors.lastname: Luo
authors.affiliation: Gansu University of Chinese Medicine, AV.Dingxi, 30, Lanzhou, 730000, PR China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Cao
authors.affiliation: Gansu University of Chinese Medicine, AV.Dingxi, 30, Lanzhou, 730000, PR China; Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicine of Gansu Province, AV. Dingxi, 30, Lanzhou, 730000, PR China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Gansu University of Chinese Medicine, AV.Dingxi, 30, Lanzhou, 730000, PR China; Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicine of Gansu Province, AV. Dingxi, 30, Lanzhou, 730000, PR China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002396:Catechols / Q000737:chemistry / Q000494:pharmacology*; D053144:Cell Nucleus Shape / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D000431:Ethanol; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D020011:Protective Agents / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism
chemical_list: D002396:Catechols; D006027:Glycosides; D020011:Protective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D016760:Proto-Oncogene Proteins c-fos; C555977:cistanoside A; D000431:Ethanol
keywords: Cistanoside A; alcohol damage; apoptosis; primary cultured hepatocytes