title: Sufentanil attenuates impairment of the endothelium-dependent vasodilation induced by hypoxia-reoxygenation in the rat coronary artery.
abstract: Sufentanil has been used broadly in cardiac surgery, but the mechanisms by which it modulates coronary vascular tone after ischemia-reperfusion injury are largely unknown. Effects of sufentanil on coronary tone and on the relaxation of rat coronary arteries (CAs) in response to endothelium-dependent (acetylcholine) and endothelium-independent (sodium nitroprusside) relaxing agents in the presence of hypoxia-reoxygenation (H/R) was studied in an in vitro organ chamber setup. Sufentanil (10-7-10-4mol/L) relaxed rat CA rings in endothelium-dependent and endothelium-independent manners. In endothelium-intact rings, preincubation of H/R-treated CAs with sufentanil (10-5mol/L) significantly increased the acetylcholine response, but did not augment sodium nitroprusside-induced relaxation. Sufentanil-mediated potentiation of acetylcholine-induced relaxation was not affected by a nitric oxide synthase inhibitor or by intermediate- or small-conductance Ca2+-activated K+channel blockers. However, potentiation was abolished by iberiotoxin (100 nmol/L), a selective inhibitor of large-conductance Ca2+-activated K+channels, as well as Rp-cAMPS (30 μmol/L), a cyclic AMP-dependent protein kinase (PKA) inhibitor. Sufentanil induced endothelium-dependent and endothelium-independent relaxation and attenuated H/R-induced impairment of endothelium-dependent vasodilation in the rat CAs. The potentiating effect of sufentanil may involve activation of large-conductance Ca2+-activated K+channels via cAMP-dependent mechanisms.
authors.affiliation: a Department of Pharmacology, Basic Medical College, Anhui Medical University, Hefei 230032, Anhui, China.|b Department of Anaesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, China.
authors.forename: Xin-Qi
authors.initials: XQ
authors.lastname: Cheng
authors.affiliation: a Department of Pharmacology, Basic Medical College, Anhui Medical University, Hefei 230032, Anhui, China.
authors.forename: Jun-Yan
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: a Department of Pharmacology, Basic Medical College, Anhui Medical University, Hefei 230032, Anhui, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Gao
authors.affiliation: b Department of Anaesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: b Department of Anaesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, China.
authors.forename: You-Mei
authors.initials: YM
authors.lastname: Zuo
authors.affiliation: b Department of Anaesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, China.
authors.forename: Er-Wei
authors.initials: EW
authors.lastname: Gu
authors.affiliation: a Department of Pharmacology, Basic Medical College, Anhui Medical University, Hefei 230032, Anhui, China.
authors.forename: Zhi-Wu
authors.initials: ZW
authors.lastname: Chen
mesh_terms: D000818:Animals; D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D003331:Coronary Vessels / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000502:physiology; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000502:physiology; D008297:Male; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D017409:Sufentanil / Q000494:pharmacology*; D014664:Vasodilation / Q000187:drug effects* / Q000502:physiology
chemical_list: D051036:Large-Conductance Calcium-Activated Potassium Channels; D017409:Sufentanil
keywords: Ca2+-activated K+ channels; canaux K+ activés par le Ca2+; dépendant de l’endothélium; endothelium-dependent; hypoxia–reoxygenation; hypoxie–réoxygénation; relaxation; sufentanil