title: Microwave-assisted extraction, HPLC analysis, and inhibitory effects on carbonic anhydrase I, II, VA, and VII isoforms of 14 blueberry Italian cultivars.
abstract: The multi-component fingerprint and the biological evaluation of plant-derived material are indispensable for the pharmaceutical field, in food quality control procedures, and in all plant-based products. We investigated the quantitative content of biologically active compounds (anthocyanins and chlorogenic acid) of microwave-assisted blueberry extracts from 14 different Italian cultivars, using validated high-performance liquid chromatography-photodiode array detector (HPLC-PDA) method and routinely instrument configuration. The carbonic anhydrase (CA, EC 4.2.1.1) inhibition profiles against several pharmacologically relevant CA isoforms of blueberry extracts and some bioactive compounds were also investigated. The various cultivars showed a highly variable content in anthocyanins and chlorogenic acid, and their CA inhibitory effects were also highly variable. Overall these data prove that antioxidant natural products found in blueberries may be useful for designing pharmacological agents in which various CAs are involved, e.g., antiobesity, antitumor, or anticonvulsants agents.
authors.affiliation: a Department of Pharmacy , "G. D'Annunzio" University of Chieti-Pescara , Chieti , Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Mollica
authors.affiliation: a Department of Pharmacy , "G. D'Annunzio" University of Chieti-Pescara , Chieti , Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Locatelli
authors.affiliation: a Department of Pharmacy , "G. D'Annunzio" University of Chieti-Pescara , Chieti , Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Macedonio
authors.affiliation: a Department of Pharmacy , "G. D'Annunzio" University of Chieti-Pescara , Chieti , Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Carradori
authors.affiliation: b Magnetic Resonance Laboratory "Annalaura Segre", Institute of Chemical Methodologies, National Research Council , Monterotondo , Rome , Italy.
authors.forename: Anatoly P
authors.initials: AP
authors.lastname: Sobolev
authors.affiliation: c CRA Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per la Frutticoltura , Rome , Italy.
authors.forename: Roberto F
authors.initials: RF
authors.lastname: De Salvador
authors.affiliation: d Istituto di Biostrutture e Bioimmagini-CNR , Naples , Italy.
authors.forename: Simona M
authors.initials: SM
authors.lastname: Monti
authors.affiliation: d Istituto di Biostrutture e Bioimmagini-CNR , Naples , Italy.|e Dipartimento di Scienze e Tecnologie Ambientali , Biologiche e Farmaceutiche, Seconda Università di Napoli , Caserta , Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Buonanno
authors.affiliation: f Department of Biology , Science Faculty, Selcuk University , Konya , Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Zengin
authors.affiliation: g Laboratorio di Chimica Bioinorganica , and.
authors.forename: Andrea
authors.initials: A
authors.lastname: Angeli
authors.affiliation: g Laboratorio di Chimica Bioinorganica , and.|h Section of Pharmaceutical and Nutriceutical Sciences, Department of Neurofarba, Università degli Studi di Firenze , Sesto Fiorentino , Florence , Italy.
authors.forename: Claudiu T
authors.initials: CT
authors.lastname: Supuran
mesh_terms: D029798:Blueberry Plants / Q000737:chemistry*; D024401:Carbonic Anhydrase I / Q000037:antagonists & inhibitors* / Q000378:metabolism; D024402:Carbonic Anhydrase II / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002257:Carbonic Anhydrase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D008872:Microwaves; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D007527:Isoenzymes; D010936:Plant Extracts; D024401:Carbonic Anhydrase I; D024402:Carbonic Anhydrase II; D002256:Carbonic Anhydrases; C477051:carbonic anhydrase VI
keywords: Blueberry; HPLC-PDA; carbonic anhydrase inhibitors; cultivar; microwave-assisted extraction