title: The photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment.
abstract: Grapevine (Vitis vinifera L.) is a species well known for its adaptation to radiation. However, photomorphogenic factors related to UV-B responses have not been molecularly characterized. We cloned and studied the role of UV-B RECEPTOR (UVR1), ELONGATED HYPOCOTYL 5 (HY5), and HY5 HOMOLOGUE (HYH) from V. vinifera We performed gene functional characterizations, generated co-expression networks, and tested them in different environmental conditions. These genes complemented the Arabidopsis uvr8 and hy5 mutants in morphological and secondary metabolic responses to radiation. We combined microarray and RNA sequencing (RNA-seq) data with promoter inspections to identify HY5 and HYH putative target genes and their DNA binding preferences. Despite sharing a large set of common co-expressed genes, we found different hierarchies for HY5 and HYH depending on the organ and stress condition, reflecting both co-operative and partially redundant roles. New candidate UV-B gene markers were supported by the presence of HY5-binding sites. These included a set of flavonol-related genes that were up-regulated in a HY5 transient expression assay. We irradiated in vitro plantlets and fruits from old potted vines with high and low UV-B exposures and followed the accumulation of flavonols and changes in gene expression in comparison with non-irradiated conditions. UVR1, HY5, and HYH expression varied with organ, developmental stage, and type of radiation. Surprisingly, UVR1 expression was modulated by shading and temperature in berries, but not by UV-B radiation. We propose that the UV-B response machinery favours berry flavonol accumulation through the activation of HY5 and HYH at different developmental stages at both high and low UV-B exposures.
authors.affiliation: Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile Departamento de Fruticultura y Enología, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Loyola
authors.affiliation: Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Daniela
authors.initials: D
authors.lastname: Herrera
authors.affiliation: Centre for Research in Agricultural Genomics-CSIC-IRTA-UAB-UB (CRAG), Campus UAB, 08193 Bellaterra, Barcelona, Spain.
authors.forename: Abraham
authors.initials: A
authors.lastname: Mas
authors.affiliation: Wine Research Centre, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Darren Chern Jan
authors.initials: DC
authors.lastname: Wong
authors.affiliation: Centre for Organismal Studies Heidelberg, University of Heidelberg, D-69120 Heidelberg, Germany.
authors.forename: Janine
authors.initials: J
authors.lastname: Höll
authors.affiliation: Department of Biotechnology, University of Verona, Italy.
authors.forename: Erika
authors.initials: E
authors.lastname: Cavallini
authors.affiliation: Department of Biotechnology, University of Verona, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Amato
authors.affiliation: Grape and Persimmon Research Division, Institute of Fruit Tree and Tea Science, NARO, Higashihiroshima, 73992494, Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Azuma
authors.affiliation: Centre for Organismal Studies Heidelberg, University of Heidelberg, D-69120 Heidelberg, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Ziegler
authors.affiliation: Laboratorio de Bioingeniería, Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez, Santiago, Chile Center for Applied Ecology and Sustainability, Santiago, Chile.
authors.forename: Felipe
authors.initials: F
authors.lastname: Aquea
authors.affiliation: Wine Research Centre, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Simone Diego
authors.initials: SD
authors.lastname: Castellarin
authors.affiliation: Centre for Organismal Studies Heidelberg, University of Heidelberg, D-69120 Heidelberg, Germany Weincampus Neustadt, DLR Rheinpfalz, D-67435 Neustadt, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Bogs
authors.affiliation: Department of Biotechnology, University of Verona, Italy.
authors.forename: Giovanni Battista
authors.initials: GB
authors.lastname: Tornielli
authors.affiliation: Departamento de Agroindustria y Enología, Facultad de Ciencias Agronómicas, Universidad de Chile, Santiago, Chile.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Peña-Neira
authors.affiliation: Quantitative Biology Center (QBIC), University of Tuebingen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Czemmel
authors.affiliation: Departamento de Fruticultura y Enología, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Santiago, Chile jalcalde@uc.cl tomas.matus@cragenomica.es parce@bio.puc.cl.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Alcalde
authors.affiliation: Centre for Research in Agricultural Genomics-CSIC-IRTA-UAB-UB (CRAG), Campus UAB, 08193 Bellaterra, Barcelona, Spain jalcalde@uc.cl tomas.matus@cragenomica.es parce@bio.puc.cl.
authors.forename: José Tomás
authors.identifier: 0000-0002-9196-1813
authors.initials: JT
authors.lastname: Matus
authors.affiliation: Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile jalcalde@uc.cl tomas.matus@cragenomica.es parce@bio.puc.cl.
authors.forename: Patricio
authors.initials: P
authors.lastname: Arce-Johnson
mesh_terms: D003001:Cloning, Molecular; D044948:Flavonols / Q000378:metabolism*; D005638:Fruit / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology / Q000528:radiation effects; D017343:Genes, Plant / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology / Q000528:radiation effects*; D014157:Transcription Factors / Q000502:physiology*; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000502:physiology / Q000528:radiation effects; D027843:Vitis / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects*
chemical_list: D044948:Flavonols; D010940:Plant Proteins; D014157:Transcription Factors
keywords: Binding; MYBF1; UVR8.; glycosyltransferase; network; photolyase; ripening