title: Effect of Huperzine A on Aβ-induced p65 of astrocyte in vitro.
abstract: Alzheimer's disease (AD) is the most common cause of dementia. Its pathology often accompanies inflammatory action, and astrocytes play important roles in such procedure. Rela(p65) is one of significant message factors in NF-κB pathway which has been reported high expression in astrocyte treated by Aβ. HupA, an alkaloid isolated from Chinese herb Huperzia serrata, has been widely used to treat AD and observations reflected that it improves memory and cognitive capacity of AD patients. To reveal its molecular mechanisms on p65, we cultured astrocytes, built Aβ-induced AD model, treated astrocytes with HupA at different concentrations, assayed cell viability with MTT, and detected p65 expression by immunohistochemistry and PCR. Our results revealed that treatment with 10 μM Aβ1-42 for 24 h induced a significant increase of NF-κB in astrocytes; HupA significantly down-regulated p65 expression induced by Aβ in astrocytes. This study infers that HupA can regulate NF-κB pathway to treat AD.
authors.affiliation: a College of Basic Medicine , Chengdu University of Traditional Chinese Medicine , Chengdu , China.
authors.forename: Lushuang
authors.initials: L
authors.lastname: Xie
authors.affiliation: a College of Basic Medicine , Chengdu University of Traditional Chinese Medicine , Chengdu , China.
authors.forename: Cen
authors.initials: C
authors.lastname: Jiang
authors.affiliation: a College of Basic Medicine , Chengdu University of Traditional Chinese Medicine , Chengdu , China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: a College of Basic Medicine , Chengdu University of Traditional Chinese Medicine , Chengdu , China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yi
authors.affiliation: a College of Basic Medicine , Chengdu University of Traditional Chinese Medicine , Chengdu , China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Gong
authors.affiliation: a College of Basic Medicine , Chengdu University of Traditional Chinese Medicine , Chengdu , China.
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Chen
authors.affiliation: b College of Sports , Southwest University for Nationalities , Chengdu , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fu
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D010446:Peptide Fragments / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000494:pharmacology*; D051996:Transcription Factor RelA / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C498144:Rela protein, mouse; D012717:Sesquiterpenes; D051996:Transcription Factor RelA; C075222:amyloid beta-protein (1-42); C050426:huperzine A
keywords: Alzheimer’s disease; Huperzine A; astrocyte; p65