title: Is there a role for stool metabolomics in cystic fibrosis?
abstract: A number of studies utilizing metabolomics have focused on the pathophysiology of cystic fibrosis (CF) lung disease. Here, we performed fecal metabolomics on pancreatic insufficient (PI) and sufficient (PS) children with CF and compared them with healthy controls (HC). Fecal metabolomics can differentiate between PS-CF and PI-CF. We identified a potential biomarker of disease severity or cystic fibrosis transmembrane conductance regulator function (m/z, 463.247; retention time, 0.570717 min) that discriminates between HC versus PS-CF versus PI-CF. We also identified lipoyl-GMP as a potential novel inflammatory biomarker, and elevation in fecal glycerol 1,2-didodecanoate 3-tetradecanoate may provide clues to the pathogenesis of intestinal inflammation. For the first time, we demonstrate the potential applications of fecal metabolomics in CF.
authors.affiliation: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Nadeem O
authors.initials: NO
authors.lastname: Kaakoush
authors.affiliation: Bioanalytical Mass Spectrometry Facility, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Russell
authors.initials: R
authors.lastname: Pickford
authors.affiliation: Discipline of Paediatrics, School of Women's and Children's Health, Medicine, University of New South Wales, Sydney, New South Wales, Australia.|Department of Paediatric Respiratory, Sydney Children's Hospital Randwick, Randwick, New South Wales, Australia.
authors.forename: Adam
authors.initials: A
authors.lastname: Jaffe
authors.affiliation: Discipline of Paediatrics, School of Women's and Children's Health, Medicine, University of New South Wales, Sydney, New South Wales, Australia.|Department of Paediatric Gastroenterology, Sydney Children's Hospital Randwick, Randwick, New South Wales, Australia.
authors.forename: Chee Y
authors.initials: CY
authors.lastname: Ooi
mesh_terms: D003550:Cystic Fibrosis / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D055432:Metabolomics / Q000379:methods*
keywords: children; cystic fibrosis; exocrine pancreatic insufficiency; inflammation; metabolomics