title: Electrochemical sensing of concanavalin A and ovalbumin interaction in solution.
abstract: In an attempt to develop a label- and reagent-free electrochemical method for the detection of lectin-glycoprotein interactions, we tested lectin-concanavalin A (ConA), glycoprotein-ovalbumin (Ova) and their complex using chronopotentiometric stripping (CPS) analysis and a hanging mercury drop electrode. Incubation of ConA with Ova resulted in an increase of the CPS peak H of the complex as compared to the CPS peaks of individual Ova and ConA proteins. Qualitatively similar results were obtained with other glycoprotein-lectin couples (ConA-RNase B and lectin from Sambucus nigra-fetuin). Using the CPS method, we were able to follow the course of complex formation in solution. Comparable responses of Ova, ConA and ConA-Ova complex were obtained not only at the mercury electrode but also with solid amalgam electrodes, which are more suitable for parallel analysis. It can be anticipated that electrochemical methods, namely CPS, will find application in glycomics and proteomics.
authors.affiliation: Institute of Biophysics, The Czech Academy of Science, v.v.i., Královopolská 135, 61265 Brno, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Vargová
authors.affiliation: Institute of Biophysics, The Czech Academy of Science, v.v.i., Královopolská 135, 61265 Brno, Czech Republic.
authors.forename: Robert
authors.initials: R
authors.lastname: Helma
authors.affiliation: Institute of Biophysics, The Czech Academy of Science, v.v.i., Královopolská 135, 61265 Brno, Czech Republic.
authors.forename: Emil
authors.initials: E
authors.lastname: Paleček
authors.affiliation: Institute of Biophysics, The Czech Academy of Science, v.v.i., Královopolská 135, 61265 Brno, Czech Republic. Electronic address: ostatna@ibp.cz.
authors.forename: Veronika
authors.initials: V
authors.lastname: Ostatná
mesh_terms: D000818:Animals; D032449:Canavalia / Q000737:chemistry; D002645:Chickens; D003208:Concanavalin A / Q000032:analysis*; D055664:Electrochemical Techniques; D008958:Models, Molecular; D010047:Ovalbumin / Q000032:analysis*; D012996:Solutions
chemical_list: D012996:Solutions; D003208:Concanavalin A; D010047:Ovalbumin
keywords: Concanavalin A; Constant current chronopotentiometric stripping; Lectin-glycoprotein interactions; Mercury electrodes; Ovalbumin; Protein-protein interactions