title: Fluorescence detection of single-nucleotide differences using aptamer-forming binary DNA probes.
abstract: We report a simple method for fluorescence detection of single-nucleotide alterations in a long target DNA, which is based on the formation of a three-way-junction-structured cholic-acid-binding DNA aptamer by the hybridization of the target with binary DNA probes. The new method was successfully exploited for SNP genotyping of human CYP2C19 gene.
authors.affiliation: Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. kato@stf.teu.ac.jp.
authors.forename: Saori
authors.initials: S
authors.lastname: Kamoto
authors.affiliation: Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. kato@stf.teu.ac.jp.
authors.forename: Masumi
authors.initials: M
authors.lastname: Hyuga
authors.affiliation: Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. kato@stf.teu.ac.jp.
authors.forename: Teru
authors.initials: T
authors.lastname: Kato
mesh_terms: D052157:Aptamers, Nucleotide; D019826:Cholic Acid / Q000737:chemistry; D065731:Cytochrome P-450 CYP2C19 / Q000235:genetics; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D060005:Genotyping Techniques; D006801:Humans; D009693:Nucleic Acid Hybridization; D020641:Polymorphism, Single Nucleotide
chemical_list: D052157:Aptamers, Nucleotide; D015342:DNA Probes; D004247:DNA; C045793:CYP2C19 protein, human; D065731:Cytochrome P-450 CYP2C19; D019826:Cholic Acid