title: Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin.
abstract: We describe refinements in optogenetic methods for circuit mapping that enable measurements of functional synaptic connectivity with single-neuron resolution. By expanding a two-photon beam in the imaging plane using the temporal focusing method and restricting channelrhodopsin to the soma and proximal dendrites, we are able to reliably evoke action potentials in individual neurons, verify spike generation with GCaMP6s, and determine the presence or absence of synaptic connections with patch-clamp electrophysiological recording.
authors.affiliation: Disorders of Neural Circuit Function, Max Planck Florida Institute for Neuroscience, Jupiter, United States.
authors.forename: Christopher A
authors.identifier: 0000-0002-0604-8449
authors.initials: CA
authors.lastname: Baker
authors.affiliation: Functional Architecture of the Cerebral Cortex, Max Planck Florida Institute for Neuroscience, Jupiter, United States.
authors.forename: Yishai M
authors.initials: YM
authors.lastname: Elyada
authors.affiliation: Functional Architecture of the Cerebral Cortex, Max Planck Florida Institute for Neuroscience, Jupiter, United States.
authors.forename: Andres
authors.initials: A
authors.lastname: Parra
authors.affiliation: Disorders of Neural Circuit Function, Max Planck Florida Institute for Neuroscience, Jupiter, United States.
authors.forename: M McLean
authors.initials: MM
authors.lastname: Bolton
mesh_terms: D000200:Action Potentials; D000818:Animals; D001931:Brain Mapping / Q000379:methods*; D008810:Mice, Inbred C57BL; D009415:Nerve Net; D062308:Optogenetics / Q000379:methods*; D018408:Patch-Clamp Techniques / Q000379:methods*
keywords: circuit; connectivity; mouse; neuroscience; optogenetics