title: Effects of maternal lead exposure on RGMa and RGMb expression in the hippocampus and cerebral cortex of mouse pups.
abstract: The present study focused on the RGM (repulsive guidance molecule) a and RGMb expression in cerebral cortex and hippocampus of newborn mice with maternal lead exposure. Lead exposure initiated from gestation to weaning. Lead acetate was dissolved in deionized water with concentrations of 0.1%, 0.2% and 0.5% groups respectively. On the 21st postnatal day, lead in blood and tissue levels was examined by GFAAS. The protein expressions of RGMa and RGMb in hippocampus and cerebral cortex tissues were tested by western blotting and immunohistochemistry. Comparing with the control group, the lead levels in blood, cerebral cortex and hippocampus of newborn mice in 0.1%, 0.2% and 0.5% lead exposure groups were remarkably higher (P<0.05). Compared with the control group, the RGMa and RGMb expression in hippocampus were upregulated in lead exposed groups (P<0.05), and the RGMa and RGMb expression in cerebral cortex were also higher (P<0.05). The increased expression of RGMa and RGMb in hippocampus and cerebral cortex may lead to the neurotoxicity in the context of maternal lead exposure.
authors.affiliation: College of Food Science and Technology, Henan Agriculture University, 450002, China. Electronic address: ln8028@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Food Science and Technology, Henan Agriculture University, 450002, China.
authors.forename: Mingwu
authors.initials: M
authors.lastname: Qiao
authors.affiliation: College of Food Science and Technology, Henan Agriculture University, 450002, China.
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: College of Food Science and Technology, Henan Agriculture University, 450002, China.
authors.forename: Pingan
authors.initials: P
authors.lastname: Zhang
authors.affiliation: College of Food Science and Technology, Henan Agriculture University, 450002, China.
authors.forename: Lianjun
authors.initials: L
authors.lastname: Song
authors.affiliation: College of Food Science and Technology, Henan Agriculture University, 450002, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Food Science and Technology, Henan Agriculture University, 450002, China.
authors.forename: Can
authors.initials: C
authors.lastname: Cui
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D015816:Cell Adhesion Molecules, Neuronal; D002540:Cerebral Cortex / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D019468:Disease Management; D005260:Female; D058851:GPI-Linked Proteins / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D007855:Lead Poisoning / Q000378:metabolism* / Q000473:pathology; D018811:Maternal Exposure; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009942:Organometallic Compounds / Q000378:metabolism / Q000633:toxicity; D011897:Random Allocation; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D058851:GPI-Linked Proteins; D009419:Nerve Tissue Proteins; D009942:Organometallic Compounds; C483496:Rgma protein, mouse; C483497:Rgmb protein, mouse; C008261:lead acetate
keywords: Cerebral cortex; Hippocampus; Lead; Neurotoxicity; RGMa; RGMb