title: Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity of Diospyros kaki Fruit and Citrus unshiu Peel.
abstract: Pancreatic lipase is the enzyme responsible for digestion and absorption of triglycerides, being its inhibition one of the widest studied methods used to determine the potential activity of natural products to inhibit dietary fat absorption. Decrease of energy intake from dietary fat through inhibition of this enzyme may be an excellent strategy to prevent and treat obesity. The inhibitory activity on pancreatic lipase enzyme of Diospyros kaki fruit and Citrus unshiu peel mixture extract (PCM) was evaluated in vitro and its antiobesity effects were studied based on the serum lipid parameters analysis from high-fat diet- (HFD-) fed mice in vivo. PCM was orally administered at a dose of 50 and 200 mg/kg body weight for 6 weeks. In addition, the activity of pancreatic lipase was assessed using orlistat (positive control). PCM exhibited inhibitory effect on lipase activity with IC50 value of 507.01 μg/mL. Moreover, serum triacylglycerol, total cholesterol levels, and visceral fat weight were significantly reduced compared to HFD control mice in PCM 200 mg/kg-treated mice (p < 0.05). These results suggest that PCM administration may be a novel potential antiobesity agent for reduction of fat absorption via inhibition of pancreatic lipase.
authors.affiliation: Department of Food Science and Biotechnology, Kyungnam University, Gyeongsangnam-do 51767, Republic of Korea.
authors.forename: Gyo-Nam
authors.initials: GN
authors.lastname: Kim
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Mi-Rae
authors.initials: MR
authors.lastname: Shin
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Shin
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Ah Reum
authors.initials: AR
authors.lastname: Lee
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Joo Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Bu-Il
authors.initials: BI
authors.lastname: Seo
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Min Yeong
authors.initials: MY
authors.lastname: Kim
authors.affiliation: Department of Food Science & Biotechnology, Daegu University, 201 Daegudae-ro, Gyeongsangbuk-do 712-714, Republic of Korea.
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Food Science & Nutrition, Tongmyong University, Busan 48520, Republic of Korea.
authors.forename: Jeong Sook
authors.initials: JS
authors.lastname: Noh
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Man Hee
authors.initials: MH
authors.lastname: Rhee
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Seong-Soo
authors.identifier: 0000-0002-4162-6849
authors.initials: SS
authors.lastname: Roh
mesh_terms: D000818:Animals; D019440:Anti-Obesity Agents / Q000737:chemistry / Q000494:pharmacology*; D002957:Citrus / Q000737:chemistry*; D059305:Diet, High-Fat / Q000009:adverse effects; D004041:Dietary Fats / Q000009:adverse effects; D029788:Diospyros / Q000737:chemistry*; D002149:Energy Intake / Q000187:drug effects; D005638:Fruit / Q000737:chemistry*; D008049:Lipase / Q000378:metabolism*; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010179:Pancreas / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D019440:Anti-Obesity Agents; D004041:Dietary Fats; D008055:Lipids; D010936:Plant Extracts; D008049:Lipase