title: SR proteins regulate V6 exon splicing of CD44 pre-mRNA.
abstract: CD44 pre-mRNA includes 20 exons, of which exons 1-5 (C1-C5) and exons 16-20 (C6-C10) are constant exons, whereas exons 6-15 (V1-V10) are variant exons. V6-exon-containing isoforms have been known to be implicated in tumor cell invasion and metastasis. In the present study, we performed a SR protein screen for CD44 V6splicing using overexpression and lentivirus-mediated shRNA treatment. Using a CD44 V6minigene, we demonstrate that increased SRSF3 and SRSF4 expression do not affect V6splicing, but increased expression of SRSF1, SRSF6 and SRSF9 significantly inhibit V6splicing. In addition, using a constitutive exon-specific primer set, we could not detect alterations of CD44 splicing after SR protein-targeting shRNA treatment. However, using a V6specific primer, we identified that reduced SRSF2 expression significantly reduced the V6isoform, but increased V6-10and V6,7-10isoforms. Our results indicate that SR proteins are important regulatory proteins for CD44 V6splicing. [BMB Reports 2016; 49(11): 612-616].
authors.affiliation: School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
authors.forename: Tiing Jen
authors.initials: TJ
authors.lastname: Loh
authors.affiliation: School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
authors.forename: Heegyum
authors.initials: H
authors.lastname: Moon
authors.affiliation: School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
authors.forename: Ha Na
authors.initials: HN
authors.lastname: Jang
authors.affiliation: School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
authors.forename: Namjeong
authors.initials: N
authors.lastname: Choi
authors.affiliation: Willston Northampton School, Easthampton, MA 01027, USA.
authors.forename: Shengfu
authors.initials: S
authors.lastname: Shen
authors.affiliation: School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
authors.forename: Darren Reece
authors.initials: DR
authors.lastname: Williams
authors.affiliation: School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
authors.forename: Da-Woon
authors.initials: DW
authors.lastname: Jung
authors.affiliation: School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
authors.forename: Xuexiu
authors.initials: X
authors.lastname: Zheng
authors.affiliation: School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
authors.forename: Haihong
authors.initials: H
authors.lastname: Shen
mesh_terms: D005091:Exons; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics* / Q000378:metabolism; D061986:MCF-7 Cells; D020033:Protein Isoforms / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D034741:RNA, Small Interfering / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D000068103:Serine-Arginine Splicing Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D020033:Protein Isoforms; D012322:RNA Precursors; D034741:RNA, Small Interfering; D000068103:Serine-Arginine Splicing Factors