title: Expanding the knowledge of the chemical structure of glycoconjugates from Trypanosoma cruzi TcI genotype. Contribution to taxonomic studies.
abstract: One of the main obstacles to the treatment of Chagas disease is the genetic and phenotypical variance displayed by T. cruzi strains, resulting in differences in morphology, virulence, pathogenicity and drug susceptibility. To better understand the role of glycoconjungates in Chagas disease, we performed the molecular characterization of the O-linked chains from mucins and glycoinositolphospholipids (GIPLs) of the Silvio X10 clone 1 strain. We demonstrated the presence of a β-galactofuranose (β-Galf) unity linked to the O-4 position of the α-N-acetylglucosamine (α-GlcNAc)O-4 in Tc-mucins. GIPLs analysis showed that the lipidic portion is exclusively composed of ceramide and the PI-oligossacharidic portion contains the Man4(AEP)GlcN-Ins-PO4 core, substituted by ethanolamine-phosphate (EtNP) on the third distal mannose from inositol, which may or may not have a terminal β Galf unity. These results confirm the classification of the Silvio X10/1 strain in group T. cruzi I. Again, it is noted that the study of T. cruzi surface glycoconjugates confirm the molecular results and the hypothesis that surface glycoconjugates may be interesting biomarker for the differentiation of trypanosomatid strains.
authors.affiliation: Laboratório de Glicobiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Av. Carlos Chagas Filho, 373, 21941902 Rio de Janeiro, RJ, Brasil.
authors.forename: Leonardo M
authors.initials: LM
authors.lastname: Fonseca
authors.affiliation: Laboratório de Glicobiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Av. Carlos Chagas Filho, 373, 21941902 Rio de Janeiro, RJ, Brasil.
authors.forename: Tatiana C
authors.initials: TC
authors.lastname: Garcez
authors.affiliation: Laboratório de Glicobiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Av. Carlos Chagas Filho, 373, 21941902 Rio de Janeiro, RJ, Brasil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Penha
authors.affiliation: Laboratório de Glicobiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Av. Carlos Chagas Filho, 373, 21941902 Rio de Janeiro, RJ, Brasil.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Freire-DE-Lima
authors.affiliation: Université des Sciences et Technologies de Lille, CNRS, UMR 8576, UGSF, Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Maes
authors.affiliation: Laboratório de Glicobiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Av. Carlos Chagas Filho, 373, 21941902 Rio de Janeiro, RJ, Brasil.
authors.forename: Kelli M
authors.initials: KM
authors.lastname: Costa
authors.affiliation: Laboratório de Glicobiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Av. Carlos Chagas Filho, 373, 21941902 Rio de Janeiro, RJ, Brasil.
authors.forename: Lucia
authors.initials: L
authors.lastname: Mendonça-Previato
authors.affiliation: Laboratório de Glicobiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Av. Carlos Chagas Filho, 373, 21941902 Rio de Janeiro, RJ, Brasil.
authors.forename: Jose O
authors.initials: JO
authors.lastname: Previato
mesh_terms: D005838:Genotype; D006001:Glycoconjugates / Q000737:chemistry*; D006017:Glycolipids / Q000737:chemistry*; D009077:Mucins / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000145:classification*
chemical_list: D006001:Glycoconjugates; D006017:Glycolipids; D009077:Mucins; D010743:Phospholipids; C115336:glycoinositolphospholipid, Trypanosoma