title: Effect of texture of platelet bags on bacterial and platelet adhesion.
abstract: Missed detection of Staphylococcus epidermidis contamination in platelet (PLT) storage bags by the standard 24-hour-postcollection BacT/ALERT screening test has been documented. A slow growth rate and the strong tendency of this bacterium to adhere to surfaces can contribute to missed detection of the pathogen.
  
  
  
  Topography of two different PLT storage bag surfaces, textured (rough) and smooth surfaces of Terumo 80440 bags (designated A15), was studied. Adhesion of biofilm-positive and -negative S. epidermidis strains on these surfaces was evaluated under static conditions. Quality of stored PLTs in A15 bags under blood bank conditions was compared for two different bag orientations (rough vs. smooth surface down) on Days 2, 5, and 7 of storage. PLT adhesion on the surfaces was evaluated after 7 days of storage.
  
  
  
  Bacterial adhesion and biofilm formation were significantly higher on the rough surfaces of A15 bags compared to the smooth surfaces. After 7 days of storage in A15 bags, PLTs showed similar metabolite levels, pH, and response capacity in the bags with different orientation and more PLT adhesion and aggregation was observed on rough surfaces.
  
  
  
  Higher bacterial adhesion on rough surfaces can contribute to missed detection of bacterial strains that tend to adhere on surfaces. PLT adhesion and aggregation on rough surfaces can affect the quality and safety of PLTs by promoting more bacterial adhesion and biofilm formation on surfaces.
authors.affiliation: Department of Chemistry, Canadian Blood Services, Vancouver, British Columbia.|Centre for Blood Research, University of British Columbia, Canadian Blood Services, Vancouver, British Columbia.
authors.forename: Narges
authors.initials: N
authors.lastname: Hadjesfandiari
authors.affiliation: Centre for Blood Research, University of British Columbia, Canadian Blood Services, Vancouver, British Columbia.|Centre for Innovation, Canadian Blood Services, Vancouver, British Columbia.|Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia.
authors.forename: Peter
authors.initials: P
authors.lastname: Schubert
authors.affiliation: Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, Canada.
authors.forename: Salma
authors.initials: S
authors.lastname: Fallah Toosi
authors.affiliation: Centre for Blood Research, University of British Columbia, Canadian Blood Services, Vancouver, British Columbia.|Centre for Innovation, Canadian Blood Services, Vancouver, British Columbia.
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Centre for Blood Research, University of British Columbia, Canadian Blood Services, Vancouver, British Columbia.|Centre for Innovation, Canadian Blood Services, Vancouver, British Columbia.
authors.forename: Brankica
authors.initials: B
authors.lastname: Culibrk
authors.affiliation: Centre for Innovation, Canadian Blood Services, Ottawa, Ontario, Canada.
authors.forename: Sandra
authors.initials: S
authors.lastname: Ramirez-Arcos
authors.affiliation: Centre for Blood Research, University of British Columbia, Canadian Blood Services, Vancouver, British Columbia.|Centre for Innovation, Canadian Blood Services, Vancouver, British Columbia.|Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia.
authors.forename: Dana V
authors.initials: DV
authors.lastname: Devine
authors.affiliation: Department of Chemistry, Canadian Blood Services, Vancouver, British Columbia.|Centre for Blood Research, University of British Columbia, Canadian Blood Services, Vancouver, British Columbia.|Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia.
authors.forename: Donald E
authors.initials: DE
authors.lastname: Brooks
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development; D001793:Blood Preservation; D006801:Humans; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D019064:Product Packaging / Q000592:standards*; D013212:Staphylococcus epidermidis / Q000166:cytology; D013499:Surface Properties; D013997:Time Factors