title: OMIP-035: Functional analysis of natural killer cell subsets in macaques.
abstract: This panel was developed to measure the functional capability of natural killer (NK) cell subsets in rhesus macaques (Macaca mulatta). It includes markers to determine the frequency of cytokine secreting and cytotoxic NK cell subpopulations in peripheral blood mononuclear cell (PBMC) samples stimulated in vitro with human 721.221 cells. NK cell subsets were defined by the expression of killer cell immunoglobulin-like receptors (KIRs) Mamu-KIR3DL01 and Mamu-KIR3DL05, and differentiation antigens CD16 and CD56. The panel can be used to assess the functional capability of NK cells in a range of normal and pathologic conditions of captive bred rhesus macaques of Indian origin. © 2016 International Society for Advancement of Cytometry.
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, 53711, Wisconsin.
authors.forename: Kim L
authors.initials: KL
authors.lastname: Weisgrau
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 53711, Wisconsin.
authors.forename: Moritz
authors.initials: M
authors.lastname: Ries
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, 53711, Wisconsin.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Pomplun
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, 53711, Wisconsin.|Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 53711, Wisconsin.
authors.forename: David T
authors.initials: DT
authors.lastname: Evans
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, 53711, Wisconsin. erakasz@primate.wisc.edu.
authors.forename: Eva G
authors.initials: EG
authors.lastname: Rakasz
mesh_terms: D000818:Animals; D019002:CD56 Antigen / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008253:Macaca mulatta / Q000097:blood* / Q000276:immunology; D017452:Receptors, IgG / Q000276:immunology; D054340:Receptors, KIR / Q000096:biosynthesis / Q000276:immunology*
chemical_list: D019002:CD56 Antigen; D018984:Epitopes, T-Lymphocyte; D017452:Receptors, IgG; D054340:Receptors, KIR
keywords: KIR3DL01; KIR3DL05; NK cell functions; rhesus macaque