title: Protective Effects of Mekabu Aqueous Solution Fermented by Lactobacillus plantarum Sanriku-SU7 on Human Enterocyte-Like HT-29-luc Cells and DSS-Induced Murine IBD Model.
abstract: Most wakame Undaria pinnatifida, a brown algae, products are made from the frond portion. In this study, the polysaccharide content and antioxidant property of aqueous extract solutions (AESs) of the four parts (frond: wakame, stem of the frond: kuki-wakame, sporophyll: mekabu, and kuki-mekabu) of wakame were investigated. Polysaccharide content was high in both the wakame and mekabu. Superoxide anion (O2-) radical-scavenging capacities were high in the mekabu. These AESs could be fermented by Lactobacillus plantarum Sanriku-SU7. The O2-radical-scavenging activity of the kuki-wakame, mekabu, and kuki-mekabu were increased by the fermentation. Fermented mekabu clearly showed a protective effect on human enterocyte-like HT-29-luc cells and in a mouse model of dextran sodium sulphate-induced inflammatory bowel disease (IBD). These results suggest that the mekabu fermented by L. plantarum Sanriku-SU7 has anti-IBD effect related to O2-radical-scavenging.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-City, Tokyo, 108-8477, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Nemoto
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-City, Tokyo, 108-8477, Japan. kuda@kaiyodai.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuda
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-City, Tokyo, 108-8477, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Eda
authors.affiliation: Office of Liaison and Cooperative Research, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-City, Tokyo, 108-8477, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamakawa
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-City, Tokyo, 108-8477, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-City, Tokyo, 108-8477, Japan.
authors.forename: Bon
authors.initials: B
authors.lastname: Kimura
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry; D016264:Dextran Sulfate / Q000009:adverse effects; D020895:Enterocytes / Q000166:cytology / Q000187:drug effects*; D005285:Fermentation; D019073:HT29 Cells; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000517:prevention & control*; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry; D044648:Undaria / Q000737:chemistry* / Q000382:microbiology
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011134:Polysaccharides; D016264:Dextran Sulfate
keywords: Human enterocyte-like HT-29-luc cells; IBD modelled mouse; Lactobacillus plantarum; Superoxide anion radical-scavenging; Undaria pinnatifida