title: Challenges for the in vivo quantification of brain neuropeptides using microdialysis sampling and LC-MS.
abstract: In recent years, neuropeptides and their receptors have received an increased interest in neuropharmacological research. Although these molecules are considered relatively small compared with proteins, their in vivo quantification using microdialysis is more challenging than for small molecules. Low microdialysis recoveries, aspecific adsorption and the presence of various multiply charged precursor ions during ESI-MS/MS detection hampers the in vivo quantification of these low abundant biomolecules. Every step in the workflow, from sampling until analysis, has to be optimized to enable the sensitive analysis of these compounds in microdialysates.
authors.affiliation: Department of Pharmaceutical Chemistry, Drug Analysis & Drug Information, Center for Neurosciences (C4N), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Van Wanseele
authors.affiliation: Department of Pharmaceutical Chemistry, Drug Analysis & Drug Information, Center for Neurosciences (C4N), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium.
authors.forename: An
authors.initials: A
authors.lastname: De Prins
authors.affiliation: Department of Pharmaceutical Chemistry, Drug Analysis & Drug Information, Center for Neurosciences (C4N), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium.
authors.forename: Dimitri
authors.initials: D
authors.lastname: De Bundel
authors.affiliation: Department of Pharmaceutical Chemistry, Drug Analysis & Drug Information, Center for Neurosciences (C4N), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium.
authors.forename: Ilse
authors.initials: I
authors.lastname: Smolders
authors.affiliation: Department of Pharmaceutical Chemistry, Drug Analysis & Drug Information, Center for Neurosciences (C4N), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium.
authors.forename: Ann
authors.initials: A
authors.lastname: Van Eeckhaut
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods*; D009479:Neuropeptides / Q000032:analysis*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
chemical_list: D009479:Neuropeptides
keywords: MS; adsorption; in vivo; microdialysis; nano LC; neuropeptides; sensitivity; superchargers