title: [Inhibitory Effect of Serum Containing Fuzheng Jiedu Decoction on the Leukemia K562/A02 Multi-drug Resistance Cells and Its Mechanism].
abstract: OBJECTIVE
  To study the inhibitory effect of serum containing Fuzheng Jiedu decoction on leukemia multi-drug-resistance K562/A02 cells and its possible mechanism.
  
  
  METHODS
  The MTT method was used to detect the inhibitory rate of K562/AO2 cells treated with serum containing Fuzheng Jiedu decoction; the flow cytometry was used to detect the inhibitory effect of serum containing medicin on growth of K562/AO2 cells and P-gp expression; the Q-PCR was used to assay the BCL-2 mRNA expression; the Western blot was used to detect the BCL-2 protein expression.
  
  
  RESULTS
  MTT cytotoxic test showed serum containing Fuzheng Jiedu decoction could inhibit K562/A02 cell growth, and the inhibitory rate increased with the increase of drug concentration; the flow cytometry showed that the serum containing Fuzheng Jiedu decoction could promote K562/A02 cell apoptosis in a concentration-dependent manner. qPCR and Western blot showed that serum containing Fuzheng Jiedu decoction could down-regulate the protein expression of BCL-2. Fuzheng Jiedu decoction could reduce the protein expression of P-gp on the K562/A02 cell membrane.
  
  
  CONCLUSIONS
  serum containing Fuzheng Jiedu decoction can promote K562/A02 cell apoptosis, its mechanism of inducing apoptosis may be related with the inhibition of BCL-2 and P-gp protein expression.
authors.affiliation: Department of Hematology, The First Hospital Affiliated to South China University, Hengyang 421001, Hunan Province, China. E-mail:474542823@qq.com.
authors.forename: Yi-Xiong
authors.initials: YX
authors.lastname: Cao
authors.affiliation: Department of Hematology, The First Hospital Affiliated to South China University, Hengyang 421001, Hunan Province, China.
authors.forename: Ze-Yu
authors.initials: ZY
authors.lastname: Luo
authors.affiliation: Department of Hematology, The First Hospital Affiliated to South China University, Hengyang 421001, Hunan Province, China.
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Li
authors.affiliation: Department of Hematology, The First Hospital Affiliated to South China University, Hengyang 421001, Hunan Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wen
authors.affiliation: Department of Hematology, The First Hospital Affiliated to South China University, Hengyang 421001, Hunan Province, China.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004365:Drugs, Chinese Herbal; D006801:Humans; D020014:K562 Cells; D007938:Leukemia
chemical_list: D004365:Drugs, Chinese Herbal; C533621:fuzheng jiedu