title: Evaluation of insertion-deletion markers suitable for genetic diversity studies and marker-trait correlation analyses in cultivated peanut (Arachis hypogaea L.).
abstract: Peanut is one of the most important oil crops worldwide. We used insertion-deletion (InDel) markers to assess the genetic diversity and population structure in cultivated peanut. Fifty-four accessions from North China were genotyped using 48 InDel markers. The markers amplified 61 polymorphic loci with 1 to 8 alleles and an average of 2.6 alleles per marker. The polymorphism information content values ranged from 0.0364 to 0.9030, with an average of 0.5038. Population structure and neighbor-joining (NJ) tree analyses suggested that all accessions could be divided into four clusters (A1-A4), using the NJ method. Likewise, four subpopulations (G1-G4) were identified using STRUCTURE analysis. A principal component analysis was also used and results concordant with the other analysis methods were found. A multi-linear stepwise regression analysis revealed that 13 InDel markers correlated with five measured agronomical traits. Our results will provide important information for future peanut molecular breeding and genetic research.
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory for Crop Germplasm Resources of Hebei, College of Agronomy, Agricultural University of Hebei, Baoding, China.
authors.forename: S
authors.initials: S
authors.lastname: Meng
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory for Crop Germplasm Resources of Hebei, College of Agronomy, Agricultural University of Hebei, Baoding, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Yang
authors.affiliation: USDA-ARS, National Peanut Research Laboratory, Dawson, GA, USA.
authors.forename: P M
authors.initials: PM
authors.lastname: Dang
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory for Crop Germplasm Resources of Hebei, College of Agronomy, Agricultural University of Hebei, Baoding, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Cui
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory for Crop Germplasm Resources of Hebei, College of Agronomy, Agricultural University of Hebei, Baoding, China.
authors.forename: G J
authors.initials: GJ
authors.lastname: Mu
authors.affiliation: Department of Crop, Soil and Environmental Sciences, Auburn University, Auburn, AL, USA.
authors.forename: C Y
authors.initials: CY
authors.lastname: Chen
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Laboratory for Crop Germplasm Resources of Hebei, College of Agronomy, Agricultural University of Hebei, Baoding, China auhpeanut@163.com.
authors.forename: L F
authors.initials: LF
authors.lastname: Liu
mesh_terms: D010367:Arachis / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D056726:Genetic Association Studies; D005819:Genetic Markers; D054643:INDEL Mutation; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers