title: Der f 34, a Novel Major House Dust Mite Allergen Belonging to a Highly Conserved Rid/YjgF/YER057c/UK114 Family of Imine Deaminases.
abstract: The high prevalence of house dust mite (HDM) allergy is a growing health problem worldwide, and the characterization of clinically important HDM allergens is a prerequisite for the development of diagnostic and therapeutic strategies. Here, we report a novel HDM allergen that belongs structurally to the highly conserved Rid/YjgF/YER057c/UK114 family (Rid family) with imine deaminase activity. Isolated HDM cDNA, named der f 34, encodes 128 amino acids homologous to Rid-like proteins. This new protein belongs to the Rid family and has seven conserved residues involved in enamine/imine deaminase activity. Indeed, we demonstrated that purified Der f 34 had imine deaminase activity that preferentially acted on leucine and methionine. Native Der f 34 showed a high IgE binding frequency as revealed by two-dimensional immunoblotting (62.5%) or ELISA (68%), which was comparable with those of a major HDM allergen Der f 2 (77.5 and 79%, respectively). We also found that Der f 34 showed cross-reactivity with another prominent indoor allergen source, Aspergillus fumigatus This is the first report showing that the Rid family imine deaminase represents an additional important pan-allergen that is conserved across organisms.
authors.affiliation: From the Hiroshima Research Center for Healthy Aging (HiHA), Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan.|the Department of Zoology, Faculty of Science, Minia University, Minia 61519, Egypt.
authors.forename: Kareem Gamal
authors.initials: KG
authors.lastname: ElRamlawy
authors.affiliation: From the Hiroshima Research Center for Healthy Aging (HiHA), Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan, fmuratks@hiroshima-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujimura
authors.affiliation: From the Hiroshima Research Center for Healthy Aging (HiHA), Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Baba
authors.affiliation: From the Hiroshima Research Center for Healthy Aging (HiHA), Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Kim
authors.affiliation: From the Hiroshima Research Center for Healthy Aging (HiHA), Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
authors.forename: Chika
authors.initials: C
authors.lastname: Kawamoto
authors.affiliation: From the Hiroshima Research Center for Healthy Aging (HiHA), Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Isobe
authors.affiliation: From the Hiroshima Research Center for Healthy Aging (HiHA), Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Abe
authors.affiliation: the Department of Microbiology, University of Georgia, Athens, Georgia 30602.
authors.forename: Kelsey
authors.initials: K
authors.lastname: Hodge-Hanson
authors.affiliation: the Department of Microbiology, University of Georgia, Athens, Georgia 30602.
authors.forename: Diana M
authors.initials: DM
authors.lastname: Downs
authors.affiliation: the Department of Zoology, Faculty of Science, Minia University, Minia 61519, Egypt.
authors.forename: Inas Hussein
authors.initials: IH
authors.lastname: Refaat
authors.affiliation: the Department of Zoology, Faculty of Science, Minia University, Minia 61519, Egypt.
authors.forename: Diaa
authors.initials: D
authors.lastname: Beshr Al-Azhary
authors.affiliation: From the Hiroshima Research Center for Healthy Aging (HiHA), Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Aki
authors.affiliation: the Takanobashi Central Hospital, Hiroshima, Hiroshima 730-0042.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Asaoku
authors.affiliation: the Takanobashi Central Hospital, Hiroshima, Hiroshima 730-0042.
authors.forename: Takaharu
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: the Katsutani Ogasawara Clinic, Hatsukaichi, Hiroshima 738-0042.
authors.forename: Takashi
authors.initials: T
authors.lastname: Katsutani
authors.affiliation: the Tsuboi Clinic, Ohtake, Hiroshima, 739-0613, and.
authors.forename: Shinji
authors.initials: S
authors.lastname: Tsuboi
authors.affiliation: From the Hiroshima Research Center for Healthy Aging (HiHA), Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan.|the Department of Food Sciences and Biotechnology, Faculty of Life Sciences, Hiroshima Institute of Technology, Hiroshima, Hiroshima 731-5193, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ono
authors.affiliation: From the Hiroshima Research Center for Healthy Aging (HiHA), Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan, skawa@hiroshima-u.ac.jp.
authors.forename: Seiji
authors.initials: S
authors.lastname: Kawamoto
mesh_terms: D000619:Aminohydrolases / Q000235:genetics / Q000276:immunology; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000235:genetics / Q000276:immunology; D060829:Arthropod Proteins / Q000235:genetics / Q000276:immunology; D001232:Aspergillus fumigatus / Q000235:genetics / Q000276:immunology; D003429:Cross Reactions; D040001:Dermatophagoides farinae / Q000235:genetics / Q000276:immunology; D005260:Female; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology; D006801:Humans; D008297:Male
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D005656:Fungal Proteins; D000619:Aminohydrolases
keywords: Aspergillus; Rid/YjgF/YER057c/UK114 family; allergen; allergy; asthma; fungi; house dust mite; imine deaminase; immunoglobulin E (IgE)