title: Glucose enhances indolic glucosinolate biosynthesis without reducing primary sulfur assimilation.
abstract: The effect of glucose as a signaling molecule on induction of aliphatic glucosinolate biosynthesis was reported in our former study. Here, we further investigated the regulatory mechanism of indolic glucosinolate biosynthesis by glucose in Arabidopsis. Glucose exerted a positive influence on indolic glucosinolate biosynthesis, which was demonstrated by induced accumulation of indolic glucosinolates and enhanced expression of related genes upon glucose treatment. Genetic analysis revealed that MYB34 and MYB51 were crucial in maintaining the basal indolic glucosinolate accumulation, with MYB34 being pivotal in response to glucose signaling. The increased accumulation of indolic glucosinolates and mRNA levels of MYB34, MYB51, and MYB122 caused by glucose were inhibited in the gin2-1 mutant, suggesting an important role of HXK1 in glucose-mediated induction of indolic glucosinolate biosynthesis. In contrast to what was known on the function of ABI5 in glucose-mediated aliphatic glucosinolate biosynthesis, ABI5 was not required for glucose-induced indolic glucosinolate accumulation. In addition, our results also indicated that glucose-induced glucosinolate accumulation was due to enhanced sulfur assimilation instead of directed sulfur partitioning into glucosinolate biosynthesis. Thus, our data provide new insights into molecular mechanisms underlying glucose-regulated glucosinolate biosynthesis.
authors.affiliation: Key Laboratory of Horticultural Plant Growth, Development and Quality improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Miao
authors.affiliation: Key Laboratory of Horticultural Plant Growth, Development and Quality improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Congxi
authors.initials: C
authors.lastname: Cai
authors.affiliation: Key Laboratory of Horticultural Plant Growth, Development and Quality improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wei
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Jirong
authors.initials: J
authors.lastname: Huang
authors.affiliation: Key Laboratory of Horticultural Plant Growth, Development and Quality improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Chang
authors.affiliation: Key Laboratory of Horticultural Plant Growth, Development and Quality improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Qian
authors.affiliation: Key Laboratory of Horticultural Plant Growth, Development and Quality improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Horticultural Plant Growth, Development and Quality improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Horticultural Plant Growth, Development and Quality improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.affiliation: Key Laboratory of Horticultural Plant Growth, Development and Quality improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Bingliang
authors.initials: B
authors.lastname: Wang
authors.affiliation: Key Laboratory of Horticultural Plant Growth, Development and Quality improvement, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.|Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D005961:Glucosinolates / Q000096:biosynthesis* / Q000378:metabolism; D007211:Indoles / Q000378:metabolism; D009154:Mutation; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D005961:Glucosinolates; D007211:Indoles; C000595373:MYB122 protein, Arabidopsis; C000595372:MYB34 protein, Arabidopsis; C523452:MYB51 protein, Arabidopsis; D014157:Transcription Factors; D013455:Sulfur; D005947:Glucose