title: Fluorescence-linked Antigen Quantification (FLAQ) Assay for Fast Quantification of HIV-1 p24Gag.
abstract: The fluorescence-linked antigen quantification (FLAQ) assay allows a fast quantification of HIV-1 p24Gagantigen. Viral supernatant are lysed and incubated with polystyrene microspheres coated with polyclonal antibodies against HIV-1 p24Gagand detector antibodies conjugated to fluorochromes (Figure 1). After washes, the fluorescence of microspheres is measured by flow cytometry and reflects the abundance of the antigen in the lysate. The speed, simplicity, and wide dynamic range of the FLAQ assay are optimum for many applications performed in HIV-1 research laboratories.
authors.affiliation: Gladstone Institutes, University of California (UCSF), San Francisco, USA.
authors.forename: Marianne
authors.initials: M
authors.lastname: Gesner
authors.affiliation: Gladstone Institutes, University of California (UCSF), San Francisco, USA.
authors.forename: Mekhala
authors.initials: M
authors.lastname: Maiti
authors.affiliation: Gladstone Institutes, University of California (UCSF), San Francisco, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Grant
authors.affiliation: Gladstone Institutes, University of California (UCSF), San Francisco, USA.
authors.forename: Marielle
authors.initials: M
authors.lastname: Cavrois