title: Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules.
abstract: Magnetic separation based on biologically-modified magnetic particles is a preconcentration procedure commonly integrated in magneto actuated platforms for the detection of a huge range of targets. However, the main drawback of this material is the low stability and high cost. In this work, a novel hybrid molecularly-imprinted polymer with magnetic properties is presented with affinity towards biotin and biotinylated biomolecules. During the synthesis of the magneto core-shell particles, biotin was used as a template. The characterization of this material by microscopy techniques including SEM, TEM and confocal microscopy is presented. The application of the magnetic-MIPs for the detection of biotin and biotinylated DNA in magneto-actuated platforms is also described for the first time. The magnetic-MIP showed a significant immobilization capacity of biotinylated molecules, giving rise to a cheaper and a robust method (it is not required to be stored at 4°C) with high binding capacity for the separation and purification under magnetic actuation of a wide range of biotinylated molecules, and their downstream application including determination of their specific targets.
authors.affiliation: Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Ben Aissa
authors.affiliation: Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Herrera-Chacon
authors.affiliation: UNESP - Univ Estadual Paulista, Institute of Chemistry, Department of Analytical Chemistry, Araraquara, SP, Brazil.
authors.forename: R R
authors.initials: RR
authors.lastname: Pupin
authors.affiliation: UNESP - Univ Estadual Paulista, Institute of Chemistry, Department of Analytical Chemistry, Araraquara, SP, Brazil.
authors.forename: M D P T
authors.initials: MDPT
authors.lastname: Sotomayor
authors.affiliation: Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Spain. Electronic address: isabel.pividori@uab.cat.
authors.forename: M I
authors.initials: MI
authors.lastname: Pividori
mesh_terms: D001710:Biotin / Q000737:chemistry / Q000302:isolation & purification*; D019921:Biotinylation; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008280:Magnetics; D059346:Magnets / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*
chemical_list: D004269:DNA, Bacterial; D011108:Polymers; D001710:Biotin
keywords: biotinylated biomolecules; immunomagnetic separation; magnetic molecularly-imprinted polymer; magnetic particles; magneto-actuated immunoassay