title: Siegesbeckia orientalis Extract Inhibits TGFβ1-Induced Migration and Invasion of Endometrial Cancer Cells.
abstract: Type II endometrial carcinoma typically exhibits aggressive metastasis and results in a poor prognosis. Siegesbeckia orientalis Linne is a traditional Chinese medicinal herb with several medicinal benefits, including the cytotoxicity against various cancers. This study investigates the inhibitory effects of S. orientalis ethanol extract (SOE) on the migration and invasion of endometrial cancer cells, which were stimulated by transforming growth factor β (TGFβ). The inhibitory effects were evaluated by determining wound healing and performing the Boyden chamber assay. This study reveals that SOE can inhibit TGFβ1-induced cell wound healing, cell migration, and cell invasion in a dose-dependent manner in RL95-2 and HEC-1A endometrial cancer cells. SOE also reversed the TGFβ1-induced epithelial-mesenchymal transition, including the loss of the cell-cell junction and the lamellipodia-like structures. Western blot analysis revealed that SOE inhibited the phosphorylation of ERK1/2, JNK1/2, and Akt, as well as the expression of MMP-9, MMP-2, and u-PA in RL95-2 cells dose-dependently. The results of this investigation suggest that SOE is a potential anti-metastatic agent against human endometrial tumors.
authors.affiliation: Department of Obstetrics & Gynecology, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan. ed101779@edah.org.tw.|Graduate Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan. ed101779@edah.org.tw.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Chang
authors.affiliation: Department of Obstetrics & Gynecology, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan. ed107312@gmail.com.|Graduate Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan. ed107312@gmail.com.
authors.forename: Xue-Hua
authors.initials: XH
authors.lastname: Ling
authors.affiliation: Department of Nutrition, I-Shou University, Kaohsiung 82445, Taiwan. fen153848@gmail.com.
authors.forename: Hsia-Fen
authors.initials: HF
authors.lastname: Hsu
authors.affiliation: Department of Obstetrics & Gynecology, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan. jingmei0112@hotmail.com.
authors.forename: Jing-Mei
authors.initials: JM
authors.lastname: Wu
authors.affiliation: Division of Cardiology, Department of Internal Medicine, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan. ed100232@edah.org.tw.
authors.forename: Chao-Ping
authors.initials: CP
authors.lastname: Wang
authors.affiliation: Graduate Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan. jfyang@isu.edu.tw.
authors.forename: Jyh-Ferng
authors.initials: JF
authors.lastname: Yang
authors.affiliation: Department of Nutrition, I-Shou University, Kaohsiung 82445, Taiwan. fanglw@isu.edu.tw.
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Fang
authors.affiliation: Graduate Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan. jyhoung@isu.edu.tw.|Department of Nutrition, I-Shou University, Kaohsiung 82445, Taiwan. jyhoung@isu.edu.tw.
authors.forename: Jer-Yiing
authors.initials: JY
authors.lastname: Houng
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D016889:Endometrial Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D000431:Ethanol / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D009361:Neoplasm Invasiveness; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000009:adverse effects*
chemical_list: D010936:Plant Extracts; D053773:Transforming Growth Factor beta1; D000431:Ethanol
keywords: Siegesbeckia orientalis linne; anti-metastasis; endometrial cancer