title: Characterization of Methanobactin from Methylosinus sp. LW4.
abstract: Methanobactins (Mbns) are a growing family of ribosomally produced, post-translationally modified natural products. Characteristic nitrogen-containing heterocycles and neighboring thioamides allow these compounds to bind copper with high affinity. Genome mining has enabled the identification of Mbn operons in bacterial genomes and the prediction of diverse Mbn structures from operon content and precursor peptide sequence. Here we report the characterization of Mbn from Methylosinus (Ms.) species (sp.) LW4. The peptide backbone is distinct from all previously characterized Mbns, and the post-translational modifications correspond precisely to those predicted on the basis of the Ms. sp. LW4 Mbn operon. Thus, prediction based on genome analysis combined with isolation and structural characterization represents a phylogenetic approach to finding diverse Mbns and elucidating their biosynthetic pathways.
authors.affiliation: Departments of †Molecular Biosciences and ‡Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
authors.forename: Grace E
authors.initials: GE
authors.lastname: Kenney
authors.affiliation: Departments of †Molecular Biosciences and ‡Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
authors.forename: Anthony W
authors.initials: AW
authors.lastname: Goering
authors.affiliation: Departments of †Molecular Biosciences and ‡Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
authors.forename: Matthew O
authors.initials: MO
authors.lastname: Ross
authors.affiliation: Departments of †Molecular Biosciences and ‡Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
authors.forename: Caroline J
authors.initials: CJ
authors.lastname: DeHart
authors.affiliation: Departments of †Molecular Biosciences and ‡Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Thomas
authors.affiliation: Departments of †Molecular Biosciences and ‡Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Hoffman
authors.affiliation: Departments of †Molecular Biosciences and ‡Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
authors.forename: Neil L
authors.initials: NL
authors.lastname: Kelleher
authors.affiliation: Departments of †Molecular Biosciences and ‡Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
authors.forename: Amy C
authors.initials: AC
authors.lastname: Rosenzweig
mesh_terms: D007093:Imidazoles / Q000737:chemistry* / Q000378:metabolism*; D020581:Methylosinus / Q000235:genetics / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009876:Operon / Q000235:genetics; D011499:Protein Processing, Post-Translational
chemical_list: D007093:Imidazoles; D009842:Oligopeptides; C491147:methanobactin