title: Differentiation potential of rabbit CD90-positive cells sorted from adipose-derived stem cells in vitro.
abstract: To investigate the differentiation potential of purified CD90+cells sorted from adipose-derived stem cells (ADSCs), CD90+cells were sorted from rabbit ADSCs using flow cytometry. Then, cell expansion of CD90+cells and unsorted ADSCs was observed using an inverted microscope. Furthermore, cell surface markers including CD40, CD105, and CD90 on CD90+cells and unsorted ADSCs were quantified using flow cytometry. Additionally, multi-lineage differentiation ability between CD90+cells and unsorted ADSCs was compared, and expression of adipocyte-related genes PPAR-r and CEBPA as well as stem cell-related gene SOX2 in CD90+cells and unsorted ADSCs was determined using real-time quantitative PCR. We found that CD90+cells had a stronger cell proliferation ability than unsorted ADSCs. CD90+cells showed a stronger ability of osteoblast and chondrocyte differentiation than unsorted ADSCs and CD90-cells, whereas the adipose differentiation ability of CD90+cells was similar to that of ADSCs and CD90-cells. CD14, CD105, and CD90 on CD90+cells were expressed more highly than those on ADSCs. Additionally, the mRNA expression level of SOX2 in CD90+cells was significantly higher than that in ADSCs, whereas the expression of PPAR-r and CEBPA was markedly lower than that in ADSCs. These results suggested that the purified CD90+cells sorted from ADSCs exhibit a stronger differentiation potential than the unsorted ADSCs.
authors.affiliation: Facility for Biochemistry and Molecular medicine Core Facilities, Zhejiang University School of Medicine, 866 Yuhangtang Rd, Hangzhou, Zhejiang, 310058, China.
authors.forename: Xinghui
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of ophthalmology, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou, 310014, Zhejiang, People's Republic of China.
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Hong
authors.affiliation: Department of ophthalmology, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou, 310014, Zhejiang, People's Republic of China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Department of ophthalmology, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou, 310014, Zhejiang, People's Republic of China.
authors.forename: Hailan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Stevenson school, 3152 Forest Lake Road, Pebble Beach, CA, 93953, USA.
authors.forename: Kangping
authors.initials: K
authors.lastname: Song
authors.affiliation: Department of ophthalmology, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou, 310014, Zhejiang, People's Republic of China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of ophthalmology, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou, 310014, Zhejiang, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Shen
authors.affiliation: Facility for Biochemistry and Molecular medicine Core Facilities, Zhejiang University School of Medicine, 866 Yuhangtang Rd, Hangzhou, Zhejiang, 310058, China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Facility for Biochemistry and Molecular medicine Core Facilities, Zhejiang University School of Medicine, 866 Yuhangtang Rd, Hangzhou, Zhejiang, 310058, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of ophthalmology, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou, 310014, Zhejiang, People's Republic of China. medicat@126.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Shen
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D049109:Cell Proliferation; D005434:Flow Cytometry / Q000379:methods*; D005786:Gene Expression Regulation; D007700:Kinetics; D008297:Male; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D018800:Thy-1 Antigens / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D018800:Thy-1 Antigens
keywords: Adipose-derived stem cell; CD90; Cell surface marker; Differentiation