title: Antiviral effects of liposome-encapsulated PolyICLC against Dengue virus in a mouse model.
abstract: This study presents the first investigation of the antiviral effects of the liposome-encapsulated PolyICLC (LE-PolyICLC) on Dengue virus (DENV) in a mouse model. In vivo efficacy studies showed that LE-PolyICLC acted to increase antiviral mechanisms mainly through promoting cytokine expression associated with innate immunity, such as IFN-γ. In addition, the pro-inflammatory cytokine TNF-α was also increased, while IL-6 level was decreased in serum. The titers of total antibodies against DENV2 in mice were also elevated. Administration of LE-PolyICLC not only alleviated the loss of body weight, degree of morbidity, and pathological damage in brains, but also reduced the viral titers and expression of viral E protein in the brain. Notably, the effectiveness of LE-PolyICLC was better than PolyICLC on the basis of the data presented in this study. These results, therefore, set a foundation for further development of LE-PolyICLC as an attractive candidate of antiviral agents to be used in both prophylactic and therapeutic settings in DENV diseases.
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Center for Molecular Medicine, Xiangya Hospital, Central South University, Changsha 410078, China. Electronic address: lunquansun@csu.edu.cn.
authors.forename: Lunquan
authors.initials: L
authors.lastname: Sun
authors.affiliation: Biotechnology, DRDC, Box 4000, Station Main, Medicine Hat, Alta T1A 8K6, Canada. Electronic address: Jonathan.Wong@drdc-rddc.gc.ca.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Wong
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Key Laboratory of Veterinary Bioproduction and Chemical Medicine of the Minstry of Agriculture, Zhongmu Institutes of China Animal Husbandry Industry Co., Ltd, No. 156 Beiqing Road, Haidian District, Beijing 100095, China. Electronic address: vetdean@cau.edu.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000956:Antigens, Viral / Q000378:metabolism; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D001921:Brain / Q000473:pathology; D002266:Carboxymethylcellulose Sodium / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D016207:Cytokines / Q000378:metabolism; D003715:Dengue / Q000188:drug therapy* / Q000821:virology*; D003716:Dengue Virus / Q000187:drug effects*; D004195:Disease Models, Animal; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D011070:Poly I-C / Q000494:pharmacology* / Q000627:therapeutic use*; D011107:Polylysine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D012367:RNA, Viral / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000956:Antigens, Viral; D000998:Antiviral Agents; D016207:Cytokines; D008081:Liposomes; D012367:RNA, Viral; D011107:Polylysine; C019531:poly ICLC; D002266:Carboxymethylcellulose Sodium; D011070:Poly I-C
keywords: Antiviral; Dengue 2 virus; Innate immunity; LE-PolyICLC