title: Association between mannose-binding lectin variants, haplotypes and risk of hepatocellular carcinoma: A case-control study.
abstract: The innate immunity gene mannose-binding lectin2 (MBL2) has played an important role in hepatitis B virus (HBV) infection, and the relationship between MBL2 variants and hepatocellular carcinoma (HCC) risk has not yet been identified. In total, 315 HCC cases and 315 healthy controls were enrolled and blood samples were acquired. High resolution melt analysis (HRM) was employed to genotype 6 polymorphisms in MBL2 gene. Increased HCC risk in carriers of LL genotype of -550 polymorphism with an adjusted OR (AOR) of 1.61 (95%CI = 1.00-2.57) was observed but no significant association detected in HL genotype. Both YX and XX genotype demonstrated a significantly elevated HCC risk in the analysis of -221 polymorphism. The B variants in codon 54 was also significantly associated with elevated HCC risk. HYB was identified as the protective factor of HCC while LXB was significantly associated with increase HCC risk. ELISA technique revealed that the MBL2 protein was significantly reduced in HCC cases. Moreover, both IL-1β and IL-6 were inversely associated with plasma MBL2 level.The mutations in MBL2 could lead to compromised innate immunity, and possibly lead to elevated HCC risk, and a novel haplotype HXB has been identified with a rate of 12.5%.
authors.affiliation: Xiamen Center for Disease Control and Prevention, Xiamen, Fujian Province, China.|School of Public Health, Xiamen University, Xiamen, Fujian Province, China.
authors.forename: Chenghao
authors.initials: C
authors.lastname: Su
authors.affiliation: Xiamen Center for Disease Control and Prevention, Xiamen, Fujian Province, China.|School of Public Health, Fujian Medical University, Fuzhou, Fujian Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Lin
authors.affiliation: School of Public Health, Fujian Medical University, Fuzhou, Fujian Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Cai
authors.affiliation: Department of hepatology, Xiamen Hospital of Traditional Chinese Medicine, Xiamen, Fujian Province, China.
authors.forename: Qianguo
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Zhongshan Hospital, Xiamen University, Xiamen, Fujian Province, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Niu
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D016015:Logistic Models; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin