title: Genome-wide Expression Analysis and Metabolite Profiling Elucidate Transcriptional Regulation of Flavonoid Biosynthesis and Modulation under Abiotic Stresses in Banana.
abstract: Flavonoid biosynthesis is largely regulated at the transcriptional level due to the modulated expression of genes related to the phenylpropanoid pathway in plants. Although accumulation of different flavonoids has been reported in banana, a staple fruit crop, no detailed information is available on regulation of the biosynthesis in this important plant. We carried out genome-wide analysis of banana (Musa acuminata, AAA genome) and identified 28 genes belonging to 9 gene families associated with flavonoid biosynthesis. Expression analysis suggested spatial and temporal regulation of the identified genes in different tissues of banana. Analysis revealed enhanced expression of genes related to flavonol and proanthocyanidin (PA) biosynthesis in peel and pulp at the early developmental stages of fruit. Genes involved in anthocyanin biosynthesis were highly expressed during banana fruit ripening. In general, higher accumulation of metabolites was observed in the peel as compared to pulp tissue. A correlation between expression of genes and metabolite content was observed at the early stage of fruit development. Furthermore, this study also suggests regulation of flavonoid biosynthesis, at transcriptional level, under light and dark exposures as well as methyl jasmonate (MJ) treatment in banana.
authors.affiliation: CSIR-National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR-NBRI), Rana Pratap Marg, Lucknow 226001, INDIA.|National Agri-Food Biotechnology Institute (NABI), Department of Biotechnology, Government of India, C-127, Industrial Area, Phase VIII, S.A.S. Nagar, Mohali 160071, India.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Pandey
authors.affiliation: National Agri-Food Biotechnology Institute (NABI), Department of Biotechnology, Government of India, C-127, Industrial Area, Phase VIII, S.A.S. Nagar, Mohali 160071, India.
authors.forename: Anshu
authors.initials: A
authors.lastname: Alok
authors.affiliation: CSIR-National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR-NBRI), Rana Pratap Marg, Lucknow 226001, INDIA.
authors.forename: Deepika
authors.initials: D
authors.lastname: Lakhwani
authors.affiliation: National Agri-Food Biotechnology Institute (NABI), Department of Biotechnology, Government of India, C-127, Industrial Area, Phase VIII, S.A.S. Nagar, Mohali 160071, India.
authors.forename: Jagdeep
authors.initials: J
authors.lastname: Singh
authors.affiliation: CSIR-National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR-NBRI), Rana Pratap Marg, Lucknow 226001, INDIA.
authors.forename: Mehar H
authors.initials: MH
authors.lastname: Asif
authors.affiliation: CSIR-National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR-NBRI), Rana Pratap Marg, Lucknow 226001, INDIA.
authors.forename: Prabodh K
authors.initials: PK
authors.lastname: Trivedi
mesh_terms: D000085:Acetates / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D005419:Flavonoids / Q000096:biosynthesis; D005638:Fruit / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D055432:Metabolomics / Q000379:methods*; D028521:Musa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics*; D044945:Proanthocyanidins / Q000096:biosynthesis; D013312:Stress, Physiological
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005419:Flavonoids; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D044945:Proanthocyanidins; C072239:methyl jasmonate