title: Advanced nitrogen removal from landfill leachate via Anammox system based on Sequencing Biofilm Batch Reactor (SBBR): Effective protection of biofilm.
abstract: High levels of organics negatively affect Anammox for treating landfill leachate. To enhance the ability of Anammox to survive against adverse environments, a lab-scale two-stage Anammox system using a Sequencing Biofilm Batch Reactor was applied to treat mature landfill leachate under 35°C. Over 107days, with influent total nitrogen (TN) and chemical oxygen demand (COD) concentrations of 3000±100 and 3000±100mg/L, effluent TN was below 20mg/L. For extracellular polymeric substance (EPS) of Anammox, slime-EPS and loosely-bound-EPS of floccules were both higher than biofilm, while tight-bound-EPS of biofilm was significantly higher, contributing to biofilm formation. Quantitative microbial analysis showed that as influent COD increased, Anammox gene ratios of biofilm increased from 1.34% to 13.28%; the gene ratios of floccule first increased, then decreased to 3.88%. This indicated that Anammox and heterotrophic bacteria could coexist because of the biofilm, leading to stable nitrogen removal performance, even when organics were present.
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Miao
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, PR China. Electronic address: wsy@bjut.edu.cn.
authors.forename: Shuying
authors.initials: S
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, PR China.
authors.forename: Tianhao
authors.initials: T
authors.lastname: Cao
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, PR China.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, PR China.
authors.forename: Zhaoyuan
authors.initials: Z
authors.lastname: Liu
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D018441:Biofilms; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D058440:Denitrification; D004867:Equipment Design; D005427:Flocculation; D052836:Heterotrophic Processes; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology; D009584:Nitrogen / Q000737:chemistry / Q000302:isolation & purification*; D014865:Waste Disposal, Fluid / Q000295:instrumentation* / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000378:metabolism
chemical_list: D014874:Water Pollutants, Chemical; D009584:Nitrogen
keywords: Anammox; Biofilm; EPS; Landfill leachate; PCR