title: Enteropathogens in children less than 5 years of age with acute diarrhea: a 5-year surveillance study in the Southeast Coast of China.
abstract: Diarrhea is the second most common cause of death among children less than 5 years of age worldwide. The etiological agents of diarrhea in the southeast coastal area of China were studied from July 2009 to December 2014.
  
  
  
  A total of the 2318 patients were enrolled in this study and examined for the presence of viruses, bacteria, and parasites. Multiplex real-time PCR was used for the detection of diarrheagenic Escherichia.coli (DEC). DEC strains were tested for susceptibility to a panel of 20 antibiotics using the Kirby-Bauer disc-diffusion method.
  
  
  
  Of the 2318 children with diarrhea, 962 (41.5 %) were positive for at least one pathogen. Rotavirus, human calicivirus (HucV), and DEC were predominant, with detection rates of 19.1 % (443), 17.7 % (411), and 7.6 % (177), respectively. The prevalences of various pathogens in patients of different ages and in different seasons were not the same. The resistance rates of 177 strains of DEC to ampicillin, tetracycline, and cefazolin were 93.2 %, 60.0 %, and 57.7 %, respectively.
  
  
  
  Rotavirus, HucV, and DEC were the main pathogens associated with diarrhea in Zhejiang, China. DEC possessed high levels of antibiotic resistance. Increased monitoring of etiological agents of diarrhea is necessary.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79, Qingchun Road, Hangzhou, 310003, People's Republic China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, No. 79, Qingchun Road, Hangzhou, 310003, People's Republic China.
authors.forename: Shufa
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, No. 79, Qingchun Road, Hangzhou, 310003, People's Republic China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79, Qingchun Road, Hangzhou, 310003, People's Republic China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, No. 79, Qingchun Road, Hangzhou, 310003, People's Republic China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, No. 79, Qingchun Road, Hangzhou, 310003, People's Republic China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Cui
authors.affiliation: Center of Clinical Laboratory, Affiliated Children's Hospital, School of Medicine, Zhejiang University, No. 57, Zhuganxiang Road, Hangzhou, 310052, People's Republic China.
authors.forename: Yongzhang
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, No. 79, Qingchun Road, Hangzhou, 310003, People's Republic China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Xie
authors.affiliation: Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, No. 79, Qingchun Road, Hangzhou, 310003, People's Republic China.
authors.forename: Xianzhi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Clinical Laboratory, Northern Jiangsu People's Hospital, No. 98, Nantong West Road, Yangzhou, 225001, People's Republic China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Han
authors.affiliation: Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, No. 79, Qingchun Road, Hangzhou, 310003, People's Republic China.
authors.forename: Yiyin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, No. 79, Qingchun Road, Hangzhou, 310003, People's Republic China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, No. 79, Qingchun Road, Hangzhou, 310003, People's Republic China. chenyu_zy@163.com.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, No. 79, Qingchun Road, Hangzhou, 310003, People's Republic China. chenyu_zy@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000667:Ampicillin; D000900:Anti-Bacterial Agents; D017250:Caliciviridae Infections / Q000453:epidemiology*; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D052978:Disk Diffusion Antimicrobial Tests; D004926:Escherichia coli / Q000502:physiology; D004927:Escherichia coli Infections / Q000453:epidemiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D011159:Population Surveillance; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D012401:Rotavirus / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology*; D012621:Seasons; D013752:Tetracycline
chemical_list: D000900:Anti-Bacterial Agents; D000667:Ampicillin; D013752:Tetracycline
keywords: Children; Diarrhea; Drug resistance; Enteropathogens