title: Molecular evolutionary analysis of the high-affinity K+ transporter gene family in angiosperms.
abstract: The high-affinity K(+) transporter (HKT) family comprises a group of multifunctional cation transporters widely distributed in organisms ranging from Bacteria to Eukarya. In angiosperms, the HKT family consists primarily of nine types, whose evolutionary relationships are not fully understood. The available sequences from 31 plant species were used to perform a comprehensive evolutionary analysis, including an examination of selection pressure and estimating phylogenetic tree and gene duplication events. Our results show that a gene duplication in the HKT1;5/HKT1;4 cluster might have led to the divergence of the HKT1;5 and HKT1;4 subfamilies. Additionally, maximum likelihood analysis revealed that the HKT family has undergone a strong purifying selection. An analysis of the amino acids provided strong statistical evidence for a functional divergence between subfamilies 1 and 2. Our study was the first to provide evidence of this functional divergence between these two subfamilies. Analysis of co-evolution in HKT identified 25 co-evolved groups. These findings expanded our understanding of the evolutionary mechanisms driving functional diversification of HKT proteins.
authors.affiliation: School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing, China.
authors.forename: P
authors.initials: P
authors.lastname: Yang
authors.affiliation: School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing, China.
authors.forename: C
authors.initials: C
authors.lastname: Hua
authors.affiliation: School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing, China.
authors.forename: F
authors.initials: F
authors.lastname: Zhou
authors.affiliation: School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing, China.
authors.forename: B-J
authors.initials: BJ
authors.lastname: Zhang
authors.affiliation: School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing, China.
authors.forename: X-N
authors.initials: XN
authors.lastname: Cai
authors.affiliation: School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing, China qzchen65@hotmail.com.
authors.forename: Q-Z
authors.initials: QZ
authors.lastname: Chen
authors.affiliation: Jiangsu Second Normal University, Nanjing, China.
authors.forename: R-L
authors.initials: RL
authors.lastname: Wang
mesh_terms: D019143:Evolution, Molecular; D020440:Gene Duplication; D016623:Ion Pumps / Q000235:genetics* / Q000378:metabolism; D019684:Magnoliopsida / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D012641:Selection, Genetic
chemical_list: D016623:Ion Pumps; D010940:Plant Proteins; D011188:Potassium