title: Echinococcus granulosus sensu stricto, Echinococcus canadensis (G7), and Echinococcus ortleppi in fertile hydatid cysts isolated from cattle in Southern Brazil.
abstract: Echinococcosis is a cosmopolitan zoonotic infection that affects humans and animals. The aim of this study was to identify and characterize the fertile hydatid cysts from bovine viscera in order to verify different species and/or genotypes present in Southern Brazil. Firstly, cysts were collected from a slaughterhouse, which received animals from different regions of Rio Grande do Sul State (RS), considered an important area of occurrence of cystic echinococcosis. In total, 2396 cysts were analyzed by microscopy to verify the presence of protoscoleces. Protoscoleces were detected in 291 samples and were classified as fertile hydatid cysts. Total DNA was extracted from protoscoleces and amplified by polymerase chain reaction (PCR). Two hundred and fifty-one samples were identified by PCR and characterized as G5/G6/G7 genotypes, of which 40 belonged to Echinococcus granulosus sensu stricto (G1-G3). PCR was also performed, using G5-specific primers to identify 250 samples as Echinococcus ortleppi (G5). Only one sample was identified as Echinococcus canadensis (G7) by DNA sequencing using primers specific for the coxI gene. Phylogenetic analysis was also performed and identified three distinct groups E1 (G5), E2 (G7), and E3 (G1-G3), which were grouped according to similarity of their sequences. The study highlights the fact that E. granulosus sensu stricto, E. ortleppi, and E. canadensis (G7) were infecting cattle in RS, emphasizing the adaptation of different species of Echinococcus to this intermediate host.
authors.affiliation: Universidade Federal de Santa Maria, UFSM, Department of Microbiology and Parasitology, Santa Maria, Brazil. Electronic address: danieliurach@gmail.com.
authors.forename: Danieli
authors.initials: D
authors.lastname: Urach Monteiro
authors.affiliation: Universidade Federal de Santa Maria, UFSM, Department of Microbiology and Parasitology, Santa Maria, Brazil.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: de Azevedo
authors.affiliation: Universidade Federal de Santa Maria, UFSM, Department of Microbiology and Parasitology, Santa Maria, Brazil.
authors.forename: Carla
authors.initials: C
authors.lastname: Weiblen
authors.affiliation: Universidade Federal de Santa Maria, UFSM, Department of Microbiology and Parasitology, Santa Maria, Brazil.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Correia Ribeiro
authors.affiliation: Universidade Federal de Santa Maria, UFSM, Department of Microbiology and Parasitology, Santa Maria, Brazil.
authors.forename: Jéssica
authors.initials: J
authors.lastname: Emmanouilidis
authors.affiliation: Universidade Federal de Santa Maria, UFSM, Department of Microbiology and Parasitology, Santa Maria, Brazil.
authors.forename: Alexandre Alberto
authors.initials: AA
authors.lastname: Tonin
authors.affiliation: Universidade Federal de Santa Maria, UFSM, Department of Microbiology and Parasitology, Santa Maria, Brazil.
authors.forename: Sônia
authors.initials: S
authors.lastname: de Avila Botton
authors.affiliation: Universidade Federal de Santa Maria, UFSM, Department of Microbiology and Parasitology, Santa Maria, Brazil.
authors.forename: Mário Luiz
authors.initials: ML
authors.lastname: de la Rue
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology*; D004443:Echinococcosis / Q000453:epidemiology* / Q000469:parasitology* / Q000662:veterinary; D004446:Echinococcus / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype
keywords: Cattle; Cystic echinococcosis; E. canadensis; E. ortleppi; Echinococcus spp.; Rio Grande do Sul