title: Neuroprotective Effects of Methyl 3,4-Dihydroxybenzoate against TBHP-Induced Oxidative Damage in SH-SY5Y Cells.
abstract: This study investigated the neuroprotective effects of methyl 3,4-dihydroxybenzoate (MDHB) against t-butyl hydroperoxide (TBHP) induced oxidative damage in SH-SY5Y (human neuroblastoma cells) and the underlying mechanisms. SH-SY5Y were cultured in DMEM + 10% FBS for 24 h and pretreated with different concentrations of MDHB or N-acetyl-l-cysteine (NAC) for 4 h prior to the addition of 40 μM TBHP for 24 h. Cell viability was analyzed using the methylthiazolyltetrazolium (MTT) and lactate dehydrogenase (LDH) assays. An annexin V-FITC assay was used to detect cell apoptosis rates. The 2',7'-dichlorofluorescin diacetate (DCFH-DA) assay was used to determine intracellular ROS levels. The activities of antioxidative enzymes (GSH-Px and SOD) were measured using commercially available kits. The oxidative DNA damage marker 8-OHdG was detected using ELISA. Western blotting was used to determine the expression of Bcl-2, Bax, caspase 3, p-Akt and Akt proteins in treated SH-SY5Y cells. Our results showed that MDHB is an effective neuroprotective compound that can mitigate oxidative stress and inhibit apoptosis in SH-SY5Y cells.
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, China. cai2011study@163.com.|School of International Education, Anhui Medical University, Hefei 230000, China. cai2011study@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Cai
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, China. wlifang001@163.com.|School of Nursing, Guangdong Pharmaceutical University, Guangzhou 510632, China. wlifang001@163.com.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, China. panjunpingjnu319@163.com.
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Pan
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, China. Mrsmanjusaka@163.com.
authors.forename: Xiang-Nan
authors.initials: XN
authors.lastname: Mi
authors.affiliation: The First Affiliated Hospital of Jinan University, Guangzhou 510632, China. zhangzhengnc@163.com.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, China. Jiapin@outlook.com.
authors.forename: Hai-Ju
authors.initials: HJ
authors.lastname: Geng
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, China. wjh8623@126.com.
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, China. jinanyaolihu326@163.com.
authors.forename: Song-Hui
authors.initials: SH
authors.lastname: Hu
authors.affiliation: Department of Pathogen Biology and Medical Immunology, School of Basic Medicine, Ningxia Medical University, Yinchuan 750021, China. zhangweisj123@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, China. tgaoq@jnu.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Anatomy, Li Ka Shing Faculty of Medicine of The University of Hong Kong, Hong Kong 999077, China. wtwu@hkucc.hku.hk.
authors.forename: Wu-Tian
authors.initials: WT
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, China. tlhm@jnu.edu.cn.|Institute of Brain Sciences, Jinan University, Guangzhou 510632, China. tlhm@jnu.edu.cn.
authors.forename: Huan-Min
authors.initials: HM
authors.lastname: Luo
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival; D004249:DNA Damage / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D062385:Hydroxybenzoates / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000009:adverse effects*
chemical_list: D062385:Hydroxybenzoates; D018696:Neuroprotective Agents; D020122:tert-Butylhydroperoxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C000600341:methyl 3,4-dihydroxybenzoate; D000111:Acetylcysteine
keywords: apoptosis; methyl 3,4-dihydroxybenzoate; neuroprotection; nuclear factor erythroid 2-related factor 2; oxidative stress