title: Measles Virus Matrix Protein Inhibits Host Cell Transcription.
abstract: Measles virus (MeV) is a highly contagious virus that still causes annual epidemics in developing countries despite the availability of a safe and effective vaccine. Additionally, importation from endemic countries causes frequent outbreaks in countries where it has been eliminated. The M protein of MeV plays a key role in virus assembly and cytopathogenesis; interestingly, M is localised in nucleus, cytoplasm and membranes of infected cells. We have used transient expression of M in transfected cells and in-cell transcription assays to show that only some MeV M localizes to the nucleus, in addition to cell membranes and the cytoplasm as previously described, and can inhibit cellular transcription via binding to nuclear factors. Additionally, MeV M was able to inhibit in vitro transcription in a dose-dependent manner. Importantly, a proportion of M is also localized to nucleus of MeV infected cells at early times in infection, correlating with inhibition of cellular transcription. Our data show, for the first time, that MeV M may play a role early in infection by inhibiting host cell transcription.
authors.affiliation: Section of Epidemiology & Statistics, Department of Public Health, Xinjiang Medical University, 393 XinYi Road, Urumqi, PR China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Yu
authors.affiliation: Respiratory Virology Group, Centre for Research in Therapeutic Solutions, Faculty of ESTeM, University of Canberra, Bruce, ACT 2617, Canberra, Australia.
authors.forename: Shadi
authors.initials: S
authors.lastname: Shahriari
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Wellington Parade, Melbourne, VIC 3800, Australia.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Li
authors.affiliation: Respiratory Virology Group, Centre for Research in Therapeutic Solutions, Faculty of ESTeM, University of Canberra, Bruce, ACT 2617, Canberra, Australia.
authors.forename: Reena
authors.initials: R
authors.lastname: Ghildyal
mesh_terms: D002462:Cell Membrane / Q000235:genetics / Q000821:virology; D002467:Cell Nucleus / Q000235:genetics; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D008457:Measles / Q000235:genetics* / Q000821:virology; D008459:Measles virus / Q000235:genetics* / Q000472:pathogenicity; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism; D019065:Virus Assembly / Q000235:genetics
chemical_list: D002843:Chromatin; D006023:Glycoproteins; D014157:Transcription Factors; D014763:Viral Matrix Proteins; C023587:protein M (glycoprotein)