title: Structural and functional properties of organic matters in extracellular polymeric substances (EPS) and dissolved organic matters (DOM) after heat pretreatment with waste sludge.
abstract: The effects of heat pretreatment on waste sludge hydrolysis were investigated in this study. Heat pretreatment was conducted at 65°C, 80°C, 100°C and 121°C for 5min, 10min, 15min, 20min, 25min and 30min. Not only analyzed the changes of SCOD (Soluble chemical oxygen demand), carbohydrate and protein, but also evaluated the structural and functional properties of organics in extracellular polymeric substances (EPS) and dissolved organic matters (DOM) by using three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectroscopy with fluorescence regional integration (FRI) analysis. The SCOD in DOM increased with pretreated temperatures. The optimal heat hydrolysis temperature and time were selected by further studying the biodegradable and non-biodegradable components. After treated at 80°C for 25min, the fluorescence intensity and percent fluorescence response (Pi,n) of easily biodegradable soluble microbial by-product substance were higher than others, and little non-biodegradable fulvic acid-like substance was accumulated.
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; Key Laboratory of Marine Environmental and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China. Electronic address: geletu@ouc.edu.cn.
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Yangguo
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Mengchun
authors.initials: M
authors.lastname: Gao
authors.affiliation: College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Zonglian
authors.initials: Z
authors.lastname: She
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Guangce
authors.initials: G
authors.lastname: Wang
mesh_terms: D057919:Biological Oxygen Demand Analysis; D005453:Fluorescence; D006358:Hot Temperature; D006868:Hydrolysis; D009930:Organic Chemicals / Q000032:analysis / Q000737:chemistry*; D011108:Polymers / Q000032:analysis / Q000737:chemistry*; D012722:Sewage / Q000032:analysis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature; D018505:Waste Management
chemical_list: D009930:Organic Chemicals; D011108:Polymers; D012722:Sewage
keywords: Dissolved organic matters (DOM); Excitation-emission matrix (EEM); Extracellular polymeric substances (EPS); Heat hydrolysis; Waste sludge