title: Encapsulation optimization of lemon balm antioxidants in calcium alginate hydrogels.
abstract: Calcium alginate hydrogel beads were used to encapsulate lemon balm extract. Chitosan layer was used to investigate the effect of hydrogel coating. To determine the interactions of antioxidant compounds of extract with encapsulation materials and its stability, microstructure of hydrogel beads was thoroughly monitored using scanning electron microscopy and Fourier transform infrared (FTIR). Total polyphenols content and antiradical activity of lemon balm extract were also evaluated before and after encapsulation. Three significant parameters (lemon balm extract, sodium alginate, and calcium chloride concentrations) were optimized by response surface methodology to obtain maximum encapsulation efficiency. The FTIR spectra showed no interactions between extract and polymers as there were no new band in spectra of alginate hydrogel after encapsulation of active compounds of lemon balm extract. The antioxidant activity of lemon balm extract did not change after encapsulation. Therefore, it was found that alginate is a suitable material for encapsulation of natural antioxidants. Sodium alginate solution concentration, 1.84%, lemon balm extract concentration, 0.4%, and calcium chloride concentration, 0.2% was determined to be the optimum condition to reach maximum encapsulation efficiency.
authors.affiliation: a Department of Food Science and Technology, College of Agriculture , Isfahan University of Technology , Isfahan , Iran.
authors.forename: Samira
authors.initials: S
authors.lastname: Najafi-Soulari
authors.affiliation: a Department of Food Science and Technology, College of Agriculture , Isfahan University of Technology , Isfahan , Iran.
authors.forename: Hajar
authors.initials: H
authors.lastname: Shekarchizadeh
authors.affiliation: a Department of Food Science and Technology, College of Agriculture , Isfahan University of Technology , Isfahan , Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Kadivar
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D002214:Capsules; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D031334:Melissa / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D000464:Alginates; D000975:Antioxidants; D002214:Capsules; D004337:Drug Carriers; D006603:Hexuronic Acids; D020100:Hydrogels; D010936:Plant Extracts; D020723:Glucuronic Acid
keywords: Lemon balm antioxidants; alginate and chitosan hydrogels; encapsulation efficiency; interactions; optimization