title: Recent advances in engineering propionyl-CoA metabolism for microbial production of value-added chemicals and biofuels.
abstract: Diminishing fossil fuel reserves and mounting environmental concerns associated with petrochemical manufacturing practices have generated significant interests in developing whole-cell biocatalytic systems for the production of value-added chemicals and biofuels. Although acetyl-CoA is a common natural biogenic precursor for the biosynthesis of numerous metabolites, propionyl-CoA is unpopular and non-native to most organisms. Nevertheless, with its C3-acyl moiety as a discrete building block, propionyl-CoA can serve as another key biogenic precursor to several biological products of industrial importance. As a result, engineering propionyl-CoA metabolism, particularly in genetically tractable hosts with the use of inexpensive feedstocks, has paved an avenue for novel biomanufacturing. Herein, we present a systematic review on manipulation of propionyl-CoA metabolism as well as relevant genetic and metabolic engineering strategies for microbial production of value-added chemicals and biofuels, including odd-chain alcohols and organic acids, bio(co)polymers and polyketides. [Formula: see text].
authors.forename: Kajan
authors.initials: K
authors.lastname: Srirangan
authors.affiliation: a Department of Chemical Engineering , University of Waterloo , Waterloo , ON , Canada.
authors.forename: Mark
authors.initials: M
authors.lastname: Bruder
authors.affiliation: a Department of Chemical Engineering , University of Waterloo , Waterloo , ON , Canada.
authors.forename: Lamees
authors.initials: L
authors.lastname: Akawi
authors.affiliation: a Department of Chemical Engineering , University of Waterloo , Waterloo , ON , Canada.
authors.forename: Dragan
authors.initials: D
authors.lastname: Miscevic
authors.affiliation: a Department of Chemical Engineering , University of Waterloo , Waterloo , ON , Canada.
authors.forename: Shane
authors.initials: S
authors.lastname: Kilpatrick
authors.affiliation: a Department of Chemical Engineering , University of Waterloo , Waterloo , ON , Canada.
authors.forename: Murray
authors.initials: M
authors.lastname: Moo-Young
authors.affiliation: a Department of Chemical Engineering , University of Waterloo , Waterloo , ON , Canada.
authors.forename: C Perry
authors.initials: CP
authors.lastname: Chou
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D056804:Biofuels; D001688:Biological Products; D060847:Metabolic Engineering
chemical_list: D000214:Acyl Coenzyme A; D056804:Biofuels; D001688:Biological Products; C009061:propionyl-coenzyme A
keywords: Biofuels; biopolymers; metabolic engineering; polyketides; propionyl-CoA; synthetic biology; value-added products