title: Screening and Identification of Highly Specific MAbs for Discovering Novel Biomarkers of Bone Marrow Stromal Cells.
abstract: Bone marrow stromal cells (BMSCs) are very useful model systems for a better understanding of cell behavior and differential gene expression. Up to now, there have not been specific markers and MAbs for BMSCs that hamper the identification and isolation of BMSCs populations. In this study, chicken BMSCs were isolated from 1-day-old Beijing fatty chickens by adherent culture. After biological characteristics were detected, the chicken BMSCs were used to immunize BALB/c mice to prepare BMSCs-specific monoclonal antibodies (MAbs) by the routine hybridoma technique. These MAbs were characterized by FACS analysis, immunocytochemistry, immunohistochemistry, subtype identification, and Western blotting assay and were used to explore markers of chicken BMSCs. Our data showed that BMSCs expressing antigens CD29, CD44, and CD105, but not expressing antigens CD34, CD45, and CD11b, could be isolated from postnatal chicken bone marrow and hold great potential for multiline age differentiation. Meanwhile, we obtained two hybridoma cell lines secreting chicken BMSCs-specific MAbs (named CHK1 and CHK2), which specifically recognized the surface antigens expressed on chicken BMSCs. According to our subtype identification, heavy chains of CHK1 and CHK2 were typed as IgG1 and IgG2b, respectively; all the light strands were kappa subtype. MAbs CHK1 and CHK2 can be used to develop the detection assay and to discover novel biomarkers of chicken BMSCs.
authors.affiliation: 1 School of Biotechnology and State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai, China .
authors.forename: Xingyuan
authors.initials: X
authors.lastname: Ma
authors.affiliation: 1 School of Biotechnology and State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai, China .|2 State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China .
authors.forename: Nanjing
authors.initials: N
authors.lastname: Lin
authors.affiliation: 1 School of Biotechnology and State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai, China .
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Kang
authors.affiliation: 1 School of Biotechnology and State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai, China .|2 State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China .
authors.forename: Linfeng
authors.initials: L
authors.lastname: Li
authors.affiliation: 3 School of Pharmacy and Shanghai Key Laboratory of New Drug Design, East China University of Science and Technology , Shanghai, China .
authors.forename: Wenyun
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology*; D015415:Biomarkers; D002454:Cell Differentiation / Q000276:immunology; D002645:Chickens / Q000276:immunology; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015415:Biomarkers