title: Mobility of lysozyme in poly(l-lysine)/hyaluronic acid multilayer films.
abstract: The spatial and temporal control over presentation of protein-based biomolecules such as growth factors and hormones is crucial for in vitro applications to mimic the complex in vivo environment. We investigated the interaction of a model protein lysozyme (Lys) with poly(L-lysine)/hyaluronic acid (PLL/HA) multilayer films. We focused on Lys diffusion as well as adsorption and retention within the film as a function of the film deposition conditions and post-treatment. Additionally, an effect of Lys concentration on its mobility was probed. A combination of confocal fluorescence microscopy, fluorescence recovery after photobleaching, and microfluidics was employed for this investigation. Our main finding is that adsorption of PLL and HA after protein loading induces acceleration and reduction of Lys mobility, respectively. These results suggest that a charge balance in the film to a high extent governs the protein-film interaction. We believe that control over protein mobility is a key to reach the full potential of the PLL/HA films as reservoirs for biomolecules depending on the application demand.
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (Fraunhofer IZI-BB), Department Cellular Biotechnology & Biochips, Am Mühlenberg 13, 14476 Potsdam-Golm, Germany; University of Potsdam, Institute for Physics and Astronomy, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, Germany.
authors.forename: Natalia
authors.initials: N
authors.lastname: Velk
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (Fraunhofer IZI-BB), Department Cellular Biotechnology & Biochips, Am Mühlenberg 13, 14476 Potsdam-Golm, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Uhlig
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (Fraunhofer IZI-BB), Department Cellular Biotechnology & Biochips, Am Mühlenberg 13, 14476 Potsdam-Golm, Germany; School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, United Kingdom.
authors.forename: Anna
authors.initials: A
authors.lastname: Vikulina
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (Fraunhofer IZI-BB), Department Cellular Biotechnology & Biochips, Am Mühlenberg 13, 14476 Potsdam-Golm, Germany.
authors.forename: Claus
authors.initials: C
authors.lastname: Duschl
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (Fraunhofer IZI-BB), Department Cellular Biotechnology & Biochips, Am Mühlenberg 13, 14476 Potsdam-Golm, Germany; School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, United Kingdom. Electronic address: dmitry.volodkin@ntu.ac.uk.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Volodkin
mesh_terms: D000327:Adsorption; D004058:Diffusion; D005453:Fluorescence; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism; D044085:Microfluidics; D018613:Microscopy, Confocal; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D011107:Polylysine / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011107:Polylysine; D006820:Hyaluronic Acid; D009113:Muramidase
keywords: Diffusion; FRAP; Layer-by-layer; Protein; Release