title: Epidermal Growth Factor Attenuates Delayed Ionizing Radiation-Induced Tissue Damage in Bone Marrow Transplanted Mice.
abstract: We examined the effect of epidermal growth factor (EGF) treatment in mice that received bone marrow transplantation (BMT) after 11 Gy whole-body irradiation. C57Bl/6 mice were divided into three treatment groups: 0 Gy; 11 Gy ((60)Co, single dose, 0.51 Gy/min) with BMT (5 × 10(6) bone marrow cells isolated from green fluorescent protein syngeneic mice, 3-4 h postirradiation); and 11 Gy with BMT and EGF (2 mg/kg applied subcutaneously 1, 3 and 5 days postirradiation). Survival data were collected. Bone marrow, peripheral blood count and cytokines, gastrointestine and liver parameters and migration of green fluorescent protein-positive cells were evaluated at 63 days postirradiation. Epidermal growth factor increased survival of irradiated animals that received BMT from 10.7 to 85.7% at 180 days postirradiation. In the BMT group, we found changes in differential bone marrow and blood count, plasma cytokine levels, gastrointestinal tissues and liver at 63 days postirradiation. These alterations were completely or in some parameters at least partially restored by epidermal growth factor. These findings indicate that epidermal growth factor, administered 1, 3 and 5 days postirradiation in combination with bone marrow transplantation, significantly improves long-term prognosis.
authors.affiliation: a   Radiobiology and.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Pejchal
authors.affiliation: a   Radiobiology and.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Sinkorova
authors.affiliation: a   Radiobiology and.|e   Biomedical Reseach Centre, University Hospital, Hradec Kralove, Czech Republic.
authors.forename: Ales
authors.initials: A
authors.lastname: Tichy
authors.affiliation: a   Radiobiology and.
authors.forename: Sarka
authors.initials: S
authors.lastname: Pruchova
authors.affiliation: a   Radiobiology and.
authors.forename: Adela
authors.initials: A
authors.lastname: Kmochova
authors.affiliation: a   Radiobiology and.
authors.forename: Kamila
authors.initials: K
authors.lastname: Durisova
authors.affiliation: a   Radiobiology and.
authors.forename: Lucie
authors.initials: L
authors.lastname: Cechakova
authors.affiliation: a   Radiobiology and.
authors.forename: Anna
authors.initials: A
authors.lastname: Lierova
authors.affiliation: a   Radiobiology and.
authors.forename: Martin
authors.initials: M
authors.lastname: Ondrej
authors.affiliation: a   Radiobiology and.
authors.forename: Marketa
authors.initials: M
authors.lastname: Nemcova
authors.affiliation: b   Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic;
authors.forename: Klara
authors.initials: K
authors.lastname: Kubelkova
authors.affiliation: c   4th Department of Internal Medicine - Haematology and.
authors.forename: Ilona
authors.initials: I
authors.lastname: Fatorova
authors.affiliation: d   2nd Department of Internal Medicine - Gastroenterology, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic; and.
authors.forename: Jan
authors.initials: J
authors.lastname: Bures
authors.affiliation: d   2nd Department of Internal Medicine - Gastroenterology, Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic; and.
authors.forename: Ilja
authors.initials: I
authors.lastname: Tacheci
authors.affiliation: e   Biomedical Reseach Centre, University Hospital, Hradec Kralove, Czech Republic.
authors.forename: Kamil
authors.initials: K
authors.lastname: Kuca
authors.affiliation: a   Radiobiology and.
authors.forename: Jirina
authors.initials: J
authors.lastname: Vavrova
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001853:Bone Marrow / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D002452:Cell Count; D016207:Cytokines / Q000097:blood; D004307:Dose-Response Relationship, Radiation; D066255:EGF Family of Proteins / Q000494:pharmacology*; D005260:Female; D007422:Intestines / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D051379:Mice; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects; D009929:Organ Size / Q000187:drug effects / Q000528:radiation effects; D011832:Radiation Injuries / Q000097:blood / Q000188:drug therapy* / Q000473:pathology / Q000628:therapy*; D012449:Safety; D013154:Spleen / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D013997:Time Factors; D014916:Whole-Body Irradiation / Q000009:adverse effects
chemical_list: D016207:Cytokines; D066255:EGF Family of Proteins