title: Immuno-Gold Labeling of Drosophila Follicles for Transmission Electron Microscopy.
abstract: Detecting the localization of cellular components using gold nanoparticles has come to offer tremendous advantages in cell biology, allowing for the high resolution imaging of the cellular organization at the subcellular level. This is further aided by the breakthroughs in the cryopreparation of samples, which focus at the retention of antigenicity in efforts to mirror the native state of the tissues and cells as closely as possible. Herein, we describe the methodology for immuno-gold labeling of Drosophila follicles, following preparation of the samples using the Tokuyasu method for ultracryosectioning.
authors.affiliation: School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Tsapras
authors.affiliation: School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK.
authors.forename: Antonia P
authors.initials: AP
authors.lastname: Sagona
authors.affiliation: School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK. I.Nezis@warwick.ac.uk.
authors.forename: Ioannis P
authors.initials: IP
authors.lastname: Nezis
mesh_terms: D000818:Animals; D016616:Cryoultramicrotomy; D004330:Drosophila / Q000648:ultrastructure*; D005260:Female; D006046:Gold; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D009866:Oogenesis; D006080:Ovarian Follicle / Q000648:ultrastructure*; D013194:Staining and Labeling
chemical_list: D006046:Gold
keywords: Cryosectioning; Drosophila eggs; Electron microscopy; Immuno-gold labeling; Oogenesis