title: Erythrina edulis (Pajuro) Seed Protein: A New Source of Antioxidant Peptides.
abstract: Erythrina edulis Triana ex Micheli is a protein-enriched legume traditionally used for both dietary and medicinal purposes. In this paper, protein concentrate was obtained from the seed flour. SDS-PAGE analysis revealed a high number and intensity of bands in the range between 10 and 90 kDa. Neutrase, Flavourzyme, and Alcalase were used to hydrolyze the protein concentrate at different times. By SDS-PAGE, the lower resistance of proteins to Alcalase action was observed, providing hydrolyzates with higher radical scavenging activity. The 120 min-hydrolyzate showed ORAC and TEAC values of 2.51 and 0.91 μmol Trolox equivalents/mg of protein, respectively. A fraction lower than 3 kDa and rich in hydrophobic and aromatic amino acids was demonstrated to be mainly responsible for the observed activity. E. edulis could be a new alternative in the formulation of functional foods not only for its high protein content but also for the potential biological properties of its hydrolyzates.
authors.forename: Arturo
authors.initials: A
authors.lastname: Intiquilla
authors.forename: Karim
authors.initials: K
authors.lastname: Jiménez-Aliaga
authors.forename: Amparo I
authors.initials: AI
authors.lastname: Zavaleta
authors.forename: Inés
authors.initials: I
authors.lastname: Arnao
authors.forename: Carmen
authors.initials: C
authors.lastname: Peña
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Chávez-Hidalgo
authors.forename: Blanca
authors.initials: B
authors.lastname: Hernández-Ledesma
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D004895:Erythrina / Q000737:chemistry*; D007700:Kinetics; D010455:Peptides / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010455:Peptides; D010940:Plant Proteins