title: Effects of zinc fluoride on inhibiting dentin demineralization and collagen degradation in vitro: A comparison of various topical fluoride agents.
abstract: Root caries is developed because of demineralization followed by enzymatic collagen degradation. This in vitro study aimed to examine the inhibitory efficacy of ZnF2on dentin demineralization and collagen degradation. Bovine dentin specimens were treated either with ZnF2or HCl-acidified ZnF2(ZnF2/HCl) and then demineralized. Anti-demineralization efficacy was assessed by TMR as mineral loss (ΔZ). The efficacy was compared with silver diammine fluoride (SDF), KF, and acidulated phosphate fluoride (APF). For evaluating anti-collagen degradation, EDTA-demineralized dentin specimens were treated by one of four fluoride agents [SDF, APF, ZnF2/HCl, NaF] followed by collagenase challenge. The eroded depth of collagen layer in the lesion was assessed using optical microscope. ΔZ of SDF, KF, ZnF2/HCl, and APF were significantly lower compared with ZnF2and Control (no treatment). Regarding anti-collagen degradation, SDF and ZnF2/HCl demonstrated a significant difference in the eroded depth compared with Control. Although SDF possessed higher efficacy, ZnF2/HCl might be beneficial as a staining-free agent.
authors.affiliation: Department of Operative Dentistry, Faculty of Dentistry, Chulalongkorn University.
authors.forename: Ornnicha
authors.initials: O
authors.lastname: Thanatvarakorn
authors.forename: Md Sofiqul
authors.initials: MS
authors.lastname: Islam
authors.forename: Syozi
authors.initials: S
authors.lastname: Nakashima
authors.forename: Alireza
authors.initials: A
authors.lastname: Sadr
authors.forename: Toru
authors.initials: T
authors.lastname: Nikaido
authors.forename: Junji
authors.initials: J
authors.lastname: Tagami
mesh_terms: D000818:Animals; D002327:Cariostatic Agents; D002417:Cattle; D003094:Collagen; D003804:Dentin; D005459:Fluorides; D005460:Fluorides, Topical; D012969:Sodium Fluoride; D017001:Tooth Demineralization; D017967:Zinc Compounds
chemical_list: D002327:Cariostatic Agents; D005460:Fluorides, Topical; D017967:Zinc Compounds; D012969:Sodium Fluoride; D003094:Collagen; C038904:zinc fluoride; D005459:Fluorides