title: Sexually Dimorphic Expression of Foxl2 and Ftz-F1 in Chinese Giant Salamander Andrias Davidianus.
abstract: Foxl2 and FTZ-F1 play a crucial role in the regulation of gonad development in fish and mammals, but studies of their function in amphibians are scarce. We isolated the full length of Foxl2 (adFoxl2) and Ftz-F1 (adFtz-f1) cDNA from the Chinese giant salamander Andrias davidianus and quantified its expression in various tissues and developing gonads. The adFoxl2 gene encodes 301aa including a conserved forkhead box, and the adFtz-f1 gene encodes 467aa containing an Ftz-F1 box. The amino acid sequences showed high homology with other amphibians. adFoxl2 expression was high in ovary, whereas adFtz-f1 was higher in testis, moderate in pituitary, ovary, and kidney; and low in the remaining tested tissues. Expression of adFoxl2 gradually increased from 1Y to 5Y in ovary, whereas adFtz-f1 expression gradually decreased in testis. In addition, adFoxl2 and adFtz-f1 were detected in granulosa cell in ovary and in spermatocytes in testis. The adFoxl2 transcription was inhibited in brain and ovary after treatment with methyltestosterone and with letrozole, whereas adFtz-f1 expression was upregulated. High-temperature suppressed the expression of adFxl2 in ovary and enhanced the transcription of adFtz-f1. These results suggest that adFoxl2 functioned in ovary differentiation, whereas adFtz-f1 played a role in testis development, which lays a foundation for study of the sex differentiation mechanism in A. davidianus.
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei, People's Republic of China.
authors.forename: Qiaomu
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei, People's Republic of China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Tian
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei, People's Republic of China.
authors.forename: Y U
authors.initials: YU
authors.lastname: Zhang
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei, People's Republic of China. xhb@yfi.ac.cn.
authors.forename: Hanbing
authors.initials: H
authors.lastname: Xiao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D018507:Gene Expression Regulation, Developmental; D000077289:Letrozole; D008297:Male; D008777:Methyltestosterone / Q000494:pharmacology; D009570:Nitriles / Q000494:pharmacology; D009928:Organ Specificity; D010053:Ovary / Q000187:drug effects / Q000254:growth & development*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012733:Sex Differentiation; D013737:Testis / Q000187:drug effects / Q000254:growth & development*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014230:Triazoles / Q000494:pharmacology; D014562:Caudata / Q000235:genetics* / Q000254:growth & development
chemical_list: D009570:Nitriles; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D014230:Triazoles; D000077289:Letrozole; D008777:Methyltestosterone