title: Nano-hydroxyapatite/β-CD/chitosan nanocomposite for potential applications in bone tissue engineering.
abstract: Herein, we report the synthesis of a novel tri-component nanocomposite system incorporating β-cyclodextrin (β-CD) with nano-hydroxyapatite (n-HA) and chitosan (CS), (n-HA/β-CD/CS) at three different temperatures via co-precipitation method. The chemical interactions and surface morphology have been evaluated by TEM, SEM and AFM techniques revealing the agglomerated nanoparticles in CS/n-HA-HA binary system whereas the ternary systems produced needle shaped nanoparticles dispersed homogeneously at low temperature with more porous and rougher surface. The addition of β-CD in CS/n-HA at low temperature decreased the particle size and raised the thermal stability as compared to CS/n-HA. The comparative hemolytic, protein adsorption and platelet adhesion studies confirmed the better hemocompatibility of n-HA/β-CD/CS-(RT,HT,LT) nanocomposites relative to CS/n-HA. The cell viability has been evaluated in vitro using MG-63 cell line which revealed superior non toxicity of n-HA/β-CD/CS-LT nanocomposite in comparison to n-HA/β-CD/CS-(RT,HT) and CS/n-HA nanocomposites. Thus it may be concluded that the orchestrated organic/inorganic n-HA/β-CD/CS-(RT,HT,LT) nanocomposites exhibited relatively higher cell viability of human osteoblast cells, stimulated greater osteogenesis, controlled biodegradation, enhanced antibacterial activity with excellent in-vitro biomineralization and remarkable mechanical parameters as compared to CS/n-HA nanocomposite and thus may provide opportunities for potential use as an alternative biomaterial for Bone tissue engineering applications.
authors.affiliation: Inorganic Chemistry Laboratory, Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India. Electronic address: shakir078@yahoo.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shakir
authors.affiliation: Inorganic Chemistry Laboratory, Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Reshma
authors.initials: R
authors.lastname: Jolly
authors.affiliation: Inorganic Chemistry Laboratory, Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Mohd Shoeb
authors.initials: MS
authors.lastname: Khan
authors.affiliation: Molecular Immunology Group Lab., Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Ahmar
authors.initials: A
authors.lastname: Rauf
authors.affiliation: Molecular Immunology Group Lab., Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Shadab
authors.initials: S
authors.lastname: Kazmi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity; D001842:Bone and Bones / Q000166:cytology*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D019245:Compressive Strength; D017886:Durapatite / Q000737:chemistry*; D006244:Hardness; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry* / Q000633:toxicity; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D047392:beta-Cyclodextrins; D048271:Chitosan; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: ALP assay; Chitosan; Heamocompatible; MTT assay; β-cyclodextrin