title: Association of endothelial nitric oxide synthase (eNOS) gene G894T polymorphism with hypertension risk and complications.
abstract: This study evaluated the association of NOS3 polymorphisms with hypertension risk and complications. eNOS (G894T) SNP was performed by RT-PCR on 70 hypertensive patients (25 were hypertensive, 25 were hypertensive with CAD, and 20 were diabetic with hypertension) and 30 age- and gender-matched individuals. Lipid and glucose profile were assessed by standard colorimetric assay. Our results revealed that combination of (GT + TT) genotype and T allele significantly increases the risk of hypertension (OR = 3.86 and 4.33), respectively. Subgroup analysis showed significant association between CAD with eNOS (G894T) mutant genotype (P = 0.002) and allele frequency (P < 0.001). Moreover, the mutant homozygous and heterozygous eNOS genotype together were significantly associated with higher TC, LDLc, (P < 0.001), and TG (P = 0.001). Thus, hypercholesterolemia (P < 0.001 and OR = 12.48) increases the risk of hypertension among T carrier. These results indicated that the T carriers significantly increase hypertension risk and complication (CAD), mainly with hypercholesterolemia and in elderly.
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Menoufia University, Shebeen El-Kom, Egypt. drabeer1975@hotmail.com.
authors.forename: Abeer A
authors.initials: AA
authors.lastname: ALrefai
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Menoufia University, Shebeen El-Kom, Egypt.
authors.forename: Mona Salah El-Din
authors.initials: MS
authors.lastname: Habib
authors.affiliation: Departments of Cardiology, Faculty of Medicine, Menoufia University, Shebeen El-Kom, Egypt.
authors.forename: Rehab I
authors.initials: RI
authors.lastname: Yaseen
authors.affiliation: Departments of Cardiology, Faculty of Medicine, Menoufia University, Shebeen El-Kom, Egypt.
authors.forename: Mahmoud K
authors.initials: MK
authors.lastname: Gabr
authors.affiliation: Departments of Anesthesia, Faculty of Medicine, Menoufia University, Shebeen El-Kom, Egypt.
authors.forename: Rabab M
authors.initials: RM
authors.lastname: Habeeb
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D019943:Amino Acid Substitution; D005260:Female; D005787:Gene Frequency; D006801:Humans; D006973:Hypertension / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011110:Polymorphism, Genetic; D012307:Risk Factors
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: CVD; Hypertension; eNOS (G894T)