title: Water interaction and bond strength to dentin of dye-labelled adhesive as a function of the addition of rhodamine B.
abstract: OBJECTIVE
  This study investigated the effect of the fluorescent dye rhodamine B (RB) for interfacial micromorphology analysis of dental composite restorations on water sorption/solubility (WS/WSL) and microtensile bond strength to dentin (µTBS) of a 3-step total etch and a 2-step self-etch adhesive system.
  
  
  METHODS
  The adhesives Adper Scotchbond Multi-Purpose (MP) and Clearfil SE Bond (SE) were mixed with 0.1 mg/mL of RB. For the WS/WSL tests, cured resin disks (5.0 mm in diameter x 0.8 mm thick) were prepared and assigned into four groups (n=10): MP, MP-RB, SE, and SE-RB. For µTBS assessment, extracted human third molars (n=40) had the flat occlusal dentin prepared and assigned into the same experimental groups (n=10). After the bonding and restoration procedures, specimens were sectioned in rectangular beams, stored in water and tested after seven days or after 12 months. The failure mode of fractured specimens was qualitatively evaluated under optical microscope (x40). Data from WS/WSL and µTBS were assessed by one-way and three-way ANOVA, respectively, and Tukey's test (α=5%).
  
  
  RESULTS
  RB increased the WSL of MP and SE. On the other hand, WS of both MP and SE was not affected by the addition of RB. No significance in µTBS between MP and MP-RB for seven days or one year was observed, whereas for SE a decrease in the µTBS means occurred in both storage times.
  
  
  CONCLUSIONS
  RB should be incorporated into non-simplified DBSs with caution, as it can interfere with their physical-mechanical properties, leading to a possible misinterpretation of bonded interface.
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Dentística, Endodontia e Materiais Odontológicos, Bauru, SP, Brasil.
authors.forename: Linda
authors.initials: L
authors.lastname: Wang
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Dentística, Endodontia e Materiais Odontológicos, Bauru, SP, Brasil.
authors.forename: Odair
authors.initials: O
authors.lastname: Bim
authors.affiliation: - Universidade de São Paulo, Hospital de Reabilitação de Anomalias Craniofaciais, Divisão de Prótese, Bauru, SP, Brasil.
authors.forename: Adolfo Coelho de Oliveira
authors.initials: AC
authors.lastname: Lopes
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia, Departamento de Dentística, São Paulo, SP, Brasil.
authors.forename: Luciana Fávaro
authors.initials: LF
authors.lastname: Francisconi-Dos-Rios
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Dentística, Endodontia e Materiais Odontológicos, Bauru, SP, Brasil.
authors.forename: Rafael Massunari
authors.initials: RM
authors.lastname: Maenosono
authors.affiliation: - Universidade Anhanguera-Bandeirantes, Grupo de Pesquisa de Biomateriais, São Paulo, SP, Brasil.
authors.forename: Paulo Henrique Perlatti
authors.initials: PH
authors.lastname: D'Alpino
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Odontopediatria, Ortodontia e Saúde Coletiva, Bauru, SP, Brasil.
authors.forename: Heitor Marques
authors.initials: HM
authors.lastname: Honório
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Dentística, Endodontia e Materiais Odontológicos, Bauru, SP, Brasil.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Atta
mesh_terms: D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods; D019232:Dental Restoration Failure; D003804:Dentin / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D018613:Microscopy, Confocal; D015203:Reproducibility of Results; D019279:Resin Cements / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*; D012995:Solubility; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: C438313:Clearfil SE Bond; D003188:Composite Resins; D017220:Dentin-Bonding Agents; C444701:Filtek Z250; D005456:Fluorescent Dyes; D019279:Resin Cements; D012235:Rhodamines; C084018:Scotchbond Multi-Purpose; D014867:Water; C029773:rhodamine B