title: aPKCζ affects directed cell migration through the regulation of myosin light chain phosphorylation.
abstract: Cell motility is an essential cellular process for a variety of biological events. It requires cross-talk between the signaling and the cytoskeletal systems. Despite the recognized importance of aPKCζ for cell motility, there is little understanding of the mechanism by which aPKCζ mediates extracellular signals to the cytoskeleton. In the present study, we report that aPKCζ is required for the cellular organization of acto-non-muscle myosin II (NMII) cytoskeleton, for proper cell adhesion and directed cell migration. We show that aPKCζ mediates EGF-dependent RhoA activation and recruitment to the cell membrane. We also show that aPKCζ mediates EGF-dependent myosin light chain (MRLC) phosphorylation that is carried out by Rho-associated protein kinase (ROCK), and that aPKCζ is required for EGF-dependent phosphorylation and inhibition of the myosin phosphatase targeting subunit (MYPT). Finally, we show that aPKCζ mediates the spatial organization of the acto-NMII cytoskeleton in response to EGF stimulation. Our data suggest that aPKCζ is an essential component regulator of acto-NMII cytoskeleton organization leading to directed cell migration, and is a mediator of the EGF signal to the cytoskeleton.
authors.affiliation: a Department of Biochemistry and Molecular Biology , The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School , Jerusalem , Israel.
authors.forename: Daria
authors.initials: D
authors.lastname: Petrov
authors.affiliation: a Department of Biochemistry and Molecular Biology , The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School , Jerusalem , Israel.
authors.forename: Inbal
authors.initials: I
authors.lastname: Dahan
authors.affiliation: a Department of Biochemistry and Molecular Biology , The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School , Jerusalem , Israel.
authors.forename: Einav
authors.initials: E
authors.lastname: Cohen-Kfir
authors.affiliation: a Department of Biochemistry and Molecular Biology , The Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School , Jerusalem , Israel.
authors.forename: Shoshana
authors.initials: S
authors.lastname: Ravid
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D051379:Mice; D008954:Models, Biological; D018994:Myosin Light Chains / Q000378:metabolism*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism*; D054460:rho-Associated Kinases / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism; D000099221:Protein Kinase C zeta
chemical_list: D004815:Epidermal Growth Factor; D018994:Myosin Light Chains; D009219:Myosin-Light-Chain Kinase; D011493:Protein Kinase C; D054460:rho-Associated Kinases; D020742:rhoA GTP-Binding Protein; D000099221:Protein Kinase C zeta
keywords: aPKCzeta; cell migration; myosin light chainsnon-muscle myosin II