title: [Effect of magnesium sulfate on MMP-9 and AQP-9 protein in placenta of patients with hypertensive disorders complicating pregnancy].
abstract: OBJECTIVE
  To investigate the protein content and protein expression of MMP-9 and AQP-9 in the placenta of patients with hypertensive disorders complicating pregnancy.And to investigate the mechanism of magnesium sulfate in the treatment of hypertensive disorders complicating pregnancy.
  
  
  METHODS
  The patients with hypertensive disorders complicating pregnancy were divided into treatment group (n=51) and untreated group (n=45), and 20 cases of normal pregnant women were selected as the control group. The three groups were taken from the placenta after delivery. ELISA and immunohistochemistry method were used to detect the protein content and protein expression of AQP-9 and MMP-9 in placenta of each group.
  
  
  RESULTS
  The protein contents and protein expression of MMP-9 in placenta tissues of treatment group were 5.89±0.52 and 125.2±0.5; AQP-9's were 4.3±0.5 and 110±4. The protein contents and protein expression of MMP-9 in placenta tissues of untreated group were 1.85±0.26 and 148.6±5.2; AQP-9's were 9.4±0.8 and 92±3. The protein contents and protein expression of MMP-9 in placenta tissues of control group were 6.13±0.84 and 120.7±4.4; AQP-9's were 4.1±0.7 and 114±3. Compared with the control group, the protein contents and protein expression of MMP-9 in placenta tissues of untreated group were significantly decreased, and that of AQP-9 was significantly increased, both with the statistically significant difference (P<0.01). The protein contents and protein expression of MMP-9 protein in placenta tissues of the treatment group were slightly lower, AQP-9 was intensity increased (both P>0.05).
  
  
  CONCLUSIONS
  Magnesium sulfate can increase the level of MMP-9 protein and down regulate the protein level of AQP-9, which may be one of the effective mechanisms of the treatment of hypertensive disorders complicating pregnancy.
authors.affiliation: Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Li
authors.forename: W Y
authors.initials: WY
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: L Y
authors.initials: LY
authors.lastname: Zou
mesh_terms: D020346:Aquaporins; D005260:Female; D006801:Humans; D046110:Hypertension, Pregnancy-Induced; D007150:Immunohistochemistry; D008278:Magnesium Sulfate; D020780:Matrix Metalloproteinase 9; D010920:Placenta; D011247:Pregnancy
chemical_list: C109669:AQP9 protein, human; D020346:Aquaporins; D008278:Magnesium Sulfate; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9