title: Identification of hydroxylation at aromatic amino acid residues in yeast kinase using mass spectrometry with affinity enrichment.
abstract: Protein kinases represent the key elements in phosphorylation-based signal transmission. Recent studies suggest that hydroxylation may mediate activities of protein kinases. This paper aims to examine the hydroxylation in protein kinases for improving our understanding of the protein modification.
  
  
  
  We combined affinity-based protein purification with MS analysis for identification of novel hydroxylation at aromatic amino acid residues in yeast kinases.
  
  
  
  We identified 17 hydroxylation at aromatic amino acid residues (10 at Phe, 1 at Tyr and 6 at Trp) using MS analysis. We further characterized the localization and studied the potential significance of these modifications.
  
  
  
  This is a new report on the identification of hydroxylation at aromatic amino acid residues in yeast kinases. This study expands the catalog of hydroxylation in kinases and suggests the potential function of hydroxylation. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Chemistry, Nankai University, Tianjin, 300071, China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Chemistry, Nankai University, Tianjin, 300071, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Bai
authors.affiliation: 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Tian
authors.affiliation: Department of Chemistry, Nankai University, Tianjin, 300071, China.
authors.forename: Guojuan
authors.initials: G
authors.lastname: Wang
authors.affiliation: 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Guijin
authors.initials: G
authors.lastname: Zhai
authors.affiliation: 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Zhenchang
authors.initials: Z
authors.lastname: Guo
authors.affiliation: 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Bi
authors.affiliation: 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Lijin
authors.initials: L
authors.lastname: Shen
authors.affiliation: 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Tianjin Key Laboratory of Medical Epigenetics, Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
mesh_terms: D024322:Amino Acids, Aromatic / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006900:Hydroxylation; D011494:Protein Kinases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D024322:Amino Acids, Aromatic; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases