title: Maternal serum thiol/disulfide homeostasis in pregnancies complicated by neural tube defects: report of a preliminary study.
abstract: OBJECTIVE
  To determine and evaluate the maternal serum thiol/disulfide homeostasis in pregnancies complicated by neural tube defects (NTD) via a novel method.
  
  
  METHODS
  Seventy-three pregnant women with NTD (study group) and seventy-one healthy control pregnant women (control group) were included in the study. A new and fully automated method was used to measure plasma native thiol, total thiol and disulfide levels, based on the reduction of dynamic disulfide bonds to functional thiol groups by sodium borohydrate.
  
  
  RESULTS
  The study and control groups were gestational age-matched. There were no statistical differences in demographic variables regarding age, gravidity, parity and body mass index. The serum native thiol levels (-SH) were 360.5 ± 50.3 and 353.3 ± 31.0 μmol/l in study and control groups, respectively, which was not statistically different (p = 0.308). The native thiol/total thiol, disulfide/native thiol and disulfide/total thiol ratios were not statistically significantly different (p > 0.05).
  
  
  CONCLUSIONS
  Our preliminary results show that maternal serum thiol/disulfide homeostatis does not change in pregnancies complicated by NTD. Larger further studies are required to evaluate the relation of oxidative stress and development of NTD.
authors.affiliation: a Department of Obstetric and Gynecology , Medical Faculty, Yuzuncu Yil University , Van , Turkey and.
authors.forename: Erbil
authors.initials: E
authors.lastname: Karaman
authors.affiliation: a Department of Obstetric and Gynecology , Medical Faculty, Yuzuncu Yil University , Van , Turkey and.
authors.forename: Orkun
authors.initials: O
authors.lastname: Çetin
authors.affiliation: a Department of Obstetric and Gynecology , Medical Faculty, Yuzuncu Yil University , Van , Turkey and.
authors.forename: Barış
authors.initials: B
authors.lastname: Boza
authors.affiliation: b Department of Biochemistry , Medical Faculty, Yıldırım Beyazit University, Ankara , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Alışık
authors.affiliation: b Department of Biochemistry , Medical Faculty, Yıldırım Beyazit University, Ankara , Turkey.
authors.forename: Özcan
authors.initials: Ö
authors.lastname: Erel
authors.affiliation: a Department of Obstetric and Gynecology , Medical Faculty, Yuzuncu Yil University , Van , Turkey and.
authors.forename: Numan
authors.initials: N
authors.lastname: Çim
authors.affiliation: a Department of Obstetric and Gynecology , Medical Faculty, Yuzuncu Yil University , Van , Turkey and.
authors.forename: Recep
authors.initials: R
authors.lastname: Yıldızhan
authors.affiliation: a Department of Obstetric and Gynecology , Medical Faculty, Yuzuncu Yil University , Van , Turkey and.
authors.forename: Hanım Güler
authors.initials: HG
authors.lastname: Şahin
mesh_terms: D000328:Adult; D004220:Disulfides / Q000097:blood; D005260:Female; D005865:Gestational Age; D006706:Homeostasis; D006801:Humans; D009436:Neural Tube Defects / Q000097:blood*; D018384:Oxidative Stress; D011247:Pregnancy; D013438:Sulfhydryl Compounds / Q000097:blood*
chemical_list: D004220:Disulfides; D013438:Sulfhydryl Compounds
keywords: Neural tube defects; oxidative stress; pregnancy; thiol/disulfide