title: Biogenic Fenton-like Reaction Involvement in Cometabolic Degradation of Tetrabromobisphenol A by Pseudomonas sp. fz.
abstract: Tetrabromobisphenol A (TBBPA) is a widely used brominated flame retardant (BFR) that has frequently been detected in various environmental compartments. Although TBBPA biotransformation has been observed under both aerobic and anaerobic conditions, knowledge of the detailed mechanism of direct aerobic TBBPA biodegradation still remains limited. In this study, the underlying mechanism of cometabolic degradation of TBBPA by Pseudomonas sp. fz under aerobic conditions was investigated. Two key degradation pathways (beta scission and debromination) were proposed based on triple quadrupole liquid chromatography-mass spectrometry (LC-MS) analysis. TBBPA degradation by strain fz was demonstrated to be an extracellular process associated with the low-molecular-mass component (LMMC). Moreover, LMMC was preliminarily identified as oligopeptides, mainly consisting of glycine, proline, and alanine in a 2:1:1 molar ratio. Quenching studies suggested the involvement of hydroxyl radicals ((•)OH) in extracellular TBBPA degradation. To the best of our knowledge, we provide the first evidence that TBBPA was degraded by a biogenic Fenton-like reaction mediated via extracellular H2O2 and Fe(II)-oligopeptide complexes by the genus Pseudomonas. This study provides a new insight into the fate and biodegradation of TBBPA and other organic pollutants in natural and artificial bioremediation environments.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology , Dalian 116024, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Gu
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology , Dalian 116024, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology , Dalian 116024, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology , Dalian 116024, China.
authors.forename: Guangfei
authors.initials: G
authors.lastname: Liu
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology , Dalian 116024, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology , Dalian 116024, China.
authors.forename: Ruofei
authors.initials: R
authors.lastname: Jin
mesh_terms: D001673:Biodegradation, Environmental; D001711:Biotransformation; D005411:Flame Retardants / Q000378:metabolism; D006861:Hydrogen Peroxide; D011075:Polybrominated Biphenyls / Q000737:chemistry; D011549:Pseudomonas / Q000378:metabolism*
chemical_list: D005411:Flame Retardants; D011075:Polybrominated Biphenyls; D006861:Hydrogen Peroxide