title: A peptidome-based phylogeny pipeline reveals differential peptides at the strain level within Bifidobacterium animalis subsp. lactis.
abstract: Bifidobacteria are gut commensal microorganisms belonging to the Actinobacteria group. Some specific strains of Bifidobacterium animalis subsp. lactis are used in functional foods as they are able to exert health-promoting effects in the human host. Due to the limited genetic variability within this subspecies, it is sometimes difficult for a manufacturer to properly track its strain once included in dairy products or functional foods. In this paper, we present a peptidome-based analysis in which the proteomes of a set of B. animalis subsp. lactis strains were digested in silico with human gut endopeptidases. The molecular masses were compared along all the strains to detect strain-specific peptides. These peptides may be interesting towards the development of methodologies for strain identification in the final product.
authors.affiliation: ESEI - Department of Computer Science, University of Vigo, Edificio Politécnico, Campus Universitario As Lagoas s/n, 32004 Ourense, Spain.
authors.forename: Aitor
authors.initials: A
authors.lastname: Blanco-Míguez
authors.affiliation: ESEI - Department of Computer Science, University of Vigo, Edificio Politécnico, Campus Universitario As Lagoas s/n, 32004 Ourense, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Gutiérrez-Jácome
authors.affiliation: ESEI - Department of Computer Science, University of Vigo, Edificio Politécnico, Campus Universitario As Lagoas s/n, 32004 Ourense, Spain.
authors.forename: Florentino
authors.initials: F
authors.lastname: Fdez-Riverola
authors.affiliation: ESEI - Department of Computer Science, University of Vigo, Edificio Politécnico, Campus Universitario As Lagoas s/n, 32004 Ourense, Spain; CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Anália
authors.initials: A
authors.lastname: Lourenço
authors.affiliation: Department of Microbiology and Biochemistry of Dairy Products, Dairy Research Institute (IPLA-CSIC), Paseo Río Linares s/n, 33300 Villaviciosa, Asturias, Spain. Electronic address: borja.sanchez@csic.es.
authors.forename: Borja
authors.initials: B
authors.lastname: Sánchez
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D000069982:Bifidobacterium animalis / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003198:Computer Simulation; D003611:Dairy Products / Q000382:microbiology; D010450:Endopeptidases / Q000737:chemistry; D016680:Genome, Bacterial; D006801:Humans; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D010802:Phylogeny; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D010455:Peptides; D020543:Proteome; D010450:Endopeptidases
keywords: Bifidobacterium animalis subsp. lactis; Peptidome; Specific peptides