title: Identification of multi-copy suppressors for endoplasmic reticulum-mitochondria tethering proteins in Saccharomyces cerevisiae.
abstract: In yeast, the endoplasmic reticulum (ER)-mitochondria encounter structure (ERMES) tethers the ER to mitochondria, but its primary function remains unclear. To gain insight into ERMES functions, we screened multi-copy suppressors of the growth-defective phenotype of mmm1∆ cells, which lack a core component of ERMES, and identified MCP1, MGA2, SPT23, and YGR250C (termed RIE1). Spt23 and Mga2 are homologous transcription factors known to activate transcription of the OLE1 gene, which encodes the fatty acid ∆9 desaturase. We found that Ole1 partially relieves the growth defects of ERMES-lacking cells, thus uncovering a relationship between fatty acid metabolism and ERMES functions.
authors.affiliation: Department of Chemistry, Graduate School of Science, Nagoya University, Japan.|Department of Material and Biological Science, Faculty of Science, Yamagata University, Japan.
authors.forename: Rieko
authors.initials: R
authors.lastname: Kojima
authors.affiliation: Department of Chemistry, Graduate School of Science, Nagoya University, Japan.
authors.forename: Shu
authors.initials: S
authors.lastname: Kajiura
authors.affiliation: Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sesaki
authors.affiliation: Department of Chemistry, Graduate School of Science, Nagoya University, Japan. endo@cc.kyoto-su.ac.jp.|Faculty of Life Sciences, Kyoto Sangyo University, Japan. endo@cc.kyoto-su.ac.jp.|JST/CREST, Kyoto Sangyo University, Japan. endo@cc.kyoto-su.ac.jp.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Endo
authors.affiliation: Department of Material and Biological Science, Faculty of Science, Yamagata University, Japan. tamura@sci.kj.yamagata-u.ac.jp.|Research Center for Materials Science, Nagoya University, Japan. tamura@sci.kj.yamagata-u.ac.jp.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tamura
mesh_terms: D004721:Endoplasmic Reticulum / Q000378:metabolism*; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D018628:Gene Dosage; D016153:Genes, Suppressor; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: C542266:MGA2 protein, S cerevisiae; C089418:MMM1 protein, S cerevisiae; D008565:Membrane Proteins; C087570:SPT23 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C029627:delta-9 fatty acid desaturase
keywords: ERMES; Saccharomyces cerevisiae; mitochondria; phospholipid