title: Thermophilic Dry Methane Fermentation of Distillation Residue Eluted from Ethanol Fermentation of Kitchen Waste and Dynamics of Microbial Communities.
abstract: Thermophilic dry methane fermentation is advantageous for feedstock with high solid content. Distillation residue with 65.1 % moisture content was eluted from ethanol fermentation of kitchen waste and subjected to thermophilic dry methane fermentation, after adjusting the moisture content to 75 %. The effect of carbon to nitrogen (C/N) ratio on thermophilic dry methane fermentation was investigated. Results showed that thermophilic dry methane fermentation could not be stably performed for >10 weeks at a C/N ratio of 12.6 and a volatile total solid (VTS) loading rate of 1 g/kg sludge/d; however, it was stably performed at a C/N ratio of 19.8 and a VTS loading rate of 3 g/kg sludge/d with 83.4 % energy recovery efficiency. Quantitative PCR analysis revealed that the number of bacteria and archaea decreased by two orders of magnitude at a C/N ratio of 12.6, whereas they were not influenced at a C/N ratio of 19.8. Microbial community analysis revealed that the relative abundance of protein-degrading bacteria increased and that of organic acid-oxidizing bacteria and acetic acid-oxidizing bacteria decreased at a C/N ratio of 12.6. Therefore, there was accumulation of NH4+and acetic acid, which inhibited thermophilic dry methane fermentation.
authors.affiliation: College of Architecture and Environment, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu, 610065, China.
authors.forename: Yu-Lian
authors.initials: YL
authors.lastname: Huang
authors.affiliation: College of Architecture and Environment, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu, 610065, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tan
authors.affiliation: College of Architecture and Environment, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu, 610065, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Wang
authors.affiliation: College of Architecture and Environment, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu, 610065, China.
authors.forename: Zhao-Yong
authors.initials: ZY
authors.lastname: Sun
authors.affiliation: College of Architecture and Environment, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu, 610065, China.
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Tang
authors.affiliation: College of Architecture and Environment, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu, 610065, China. kida@gpo.kumamoto-u.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kida
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000737:chemistry / Q000378:metabolism*; D001419:Bacteria / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002244:Carbon / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D005285:Fermentation; D008697:Methane / Q000737:chemistry / Q000378:metabolism*; D009584:Nitrogen / Q000737:chemistry; D012037:Refuse Disposal / Q000379:methods; D012722:Sewage / Q000737:chemistry; D062611:Solid Waste; D013696:Temperature
chemical_list: D012722:Sewage; D062611:Solid Waste; D000431:Ethanol; D002244:Carbon; D009584:Nitrogen; D008697:Methane
keywords: Anaerobic digestion; Dry methane fermentation; Ethanol fermentation; Kitchen waste; Stillage