title: Transcription factors mediate condensin recruitment and global chromosomal organization in fission yeast.
abstract: It is becoming clear that structural-maintenance-of-chromosomes (SMC) complexes such as condensin and cohesin are involved in three-dimensional genome organization, yet their exact roles in functional organization remain unclear. We used chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) to comprehensively identify genome-wide associations mediated by condensin and cohesin in fission yeast. We found that although cohesin and condensin often bind to the same loci, they direct different association networks and generate small and larger chromatin domains, respectively. Cohesin mediates associations between loci positioned within 100 kb of each other; condensin can drive longer-range associations. Moreover, condensin, but not cohesin, connects cell cycle-regulated genes bound by mitotic transcription factors. This study describes the different functions of condensin and cohesin in genome organization and how specific transcription factors function in condensin loading, cell cycle-dependent genome organization and mitotic chromosome organization to support faithful chromosome segregation.
authors.affiliation: The Wistar Institute, Philadelphia, Pennsylvania, USA.
authors.forename: Kyoung-Dong
authors.initials: KD
authors.lastname: Kim
authors.affiliation: The Wistar Institute, Philadelphia, Pennsylvania, USA.
authors.forename: Hideki
authors.initials: H
authors.lastname: Tanizawa
authors.affiliation: The Wistar Institute, Philadelphia, Pennsylvania, USA.
authors.forename: Osamu
authors.initials: O
authors.lastname: Iwasaki
authors.affiliation: The Wistar Institute, Philadelphia, Pennsylvania, USA.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Noma
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D015825:Chromosomes, Fungal; D004268:DNA-Binding Proteins / Q000378:metabolism*; D050980:GATA Transcription Factors / Q000378:metabolism*; D005800:Genes, Fungal; D018816:Genes, cdc; D008938:Mitosis; D046912:Multiprotein Complexes / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D000072417:Protein Domains; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D000097722:Cohesins
chemical_list: D000251:Adenosine Triphosphatases; D018797:Cell Cycle Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D050980:GATA Transcription Factors; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D010750:Phosphoproteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D000097722:Cohesins; C502932:Ace2 protein, S pombe; C472167:Ams2 protein, S pombe; C092957:Rad21 protein, S pombe; C108312:condensin complexes; C090209:cut14 protein, S pombe