title: [Relationship between the mode of HBV marker and immune status in neonates and non-/hyporesponse to hepatitis B vaccine].
abstract: OBJECTIVE
  A prospective study was conducted to explore the influence of neonatal modes of HBV marker (HBVM) on non-/hypo-response to hepatitis B vaccine in infants.
  
  
  METHODS
  From July 2011 to July 2013, a total of 386 pregnant women who showed serum HBsAg positive with their neonates at birth and another 227 infants at 12 months admitted in the Third People' s Hospital of Taiyuan in Shanxi province, China. All infants received hepatitis B vaccine with the 0-1-6 month schedule. Maternal, neonatal and infantile HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc were measured by chemiluminescence-immunoassay. The neonatal/infantile PBMC TLR3 expression level and the quantities of T cell subsets, B cells, DCs were measured by Flow Cytometry. The neonatal/infantile Th1/Th2 cytokines were measured by ELISA.
  
  
  RESULTS
  Four types of common neonatal modes of HBVM appeared as " HBeAg(+) anti-HBe(+) " , "HBsAg(+)HBeAg (+) anti-HBe(+) " , "HBsAg(+) " and "HBVM(-)" , respectively. The overall rate of non-/hypo-response to hepatitis B vaccine in neonatal mode of " HBeAg(+) anti-HBe(+) " was 5.2%, lower than that seen in the other three types of mode (20.0%, 40.0% and 22.5%, respectively). The frequencies of circulating CD4(+) T cells and CD8(+) T cells were significantly different among four common modes of HBVM in infants. Meanwhile, the level of IL-6 in mode of " HBeAg(+) anti-HBe(+) " was higher than that in the mode of " HBVM(-)" at two points. There was a positive correlation appeared between the level of IL-6 and the level of anti-HBs. It was quite unlikely to show non-/hypo-response to hepatitis B vaccine, when neonates were at the level as IL-6> 1 112.0 pg/ml (OR=0.386, 95% CI: 0.266-0.561, P<0.001).
  
  
  CONCLUSIONS
  Neonates who were with the mode of " HBeAg (+) anti-HBe (+) " and high level of IL-6 showed a lower non-/hyporesponse rate on hepatitis B vaccine. It is necessary to further study the relationship between neonatal mode of HBVM and the immune status.
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: S P
authors.initials: SP
authors.lastname: Wang
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Shi
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Wang
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: L R
authors.initials: LR
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: H X
authors.initials: HX
authors.lastname: Wen
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: X X
authors.initials: XX
authors.lastname: Xu
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Yang
authors.affiliation: Obstetrics and Gynaecology Department, Third People Hospital of Taiyuan City, Taiyuan 030001, China.
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.affiliation: Obstetrics and Gynaecology Department, Third People Hospital of Taiyuan City, Taiyuan 030001, China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Feng
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D018414:CD8-Positive T-Lymphocytes; D002681:China; D005260:Female; D006509:Hepatitis B / Q000097:blood / Q000188:drug therapy* / Q000276:immunology / Q000517:prevention & control*; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000097:blood; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D006515:Hepatitis B virus; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015850:Interleukin-6; D007963:Leukocytes, Mononuclear; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D011247:Pregnancy; D011446:Prospective Studies
chemical_list: D015415:Biomarkers; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D015850:Interleukin-6