title: Inactivated porcine reproductive and respiratory syndrome virus vaccine adjuvanted with Montanide™ Gel 01 ST elicits virus-specific cross-protective inter-genotypic response in piglets.
abstract: The efficacy of a novel BEI-inactivated porcine reproductive and respiratory syndrome virus (PRRSV) candidate vaccine in pigs, developed at RIBSP Republic of Kazakhstan and delivered with an adjuvant Montanide™ Gel 01 ST (D/KV/ADJ) was compared with a commercial killed PRRSV vaccine (NVDC-JXA1, C/KV/ADJ) used widely in swine herds of the Republic of Kazakhstan. Clinical parameters (body temperature and respiratory disease scores), virological and immunological profiles [ELISA and virus neutralizing (VN) antibody titers], macroscopic lung lesions and viral load in the lungs (quantitative real-time PCR and cell culture assay) were assessed in vaccinated and both genotype 1 and 2 PRRSV challenged pigs. Our results showed that the commercial vaccine failed to protect pigs adequately against the clinical disease, viremia and lung lesions caused by the challenged field isolates, Kazakh strains of PRRSV type 1 and type 2 genotypes. In contrast, clinical protection, absence of viremia and lung lesions in D/KV/ADJ vaccinated pigs was associated with generation of VN antibodies in both homologous vaccine strain LKZ/2010 (PRRSV type 2) and a heterogeneous type 1 PRRSV strain (CM/08) challenged pigs. Thus, our data indicated the induction of cross-protective VN antibodies by D/KV/ADJ vaccine, and importantly demonstrated that an inactivated PRRSV vaccine could also induce cross-protective response across the viral genotype.
authors.affiliation: Research Institute for Biological Safety Problems (RIBSP), Science Committee, Ministry of Education and Science of the Republic of Kazakhstan, Zhambylskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan. Electronic address: tabynov777@mail.ru.
authors.forename: Kairat
authors.initials: K
authors.lastname: Tabynov
authors.affiliation: Research Institute for Biological Safety Problems (RIBSP), Science Committee, Ministry of Education and Science of the Republic of Kazakhstan, Zhambylskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
authors.forename: Abylay
authors.initials: A
authors.lastname: Sansyzbay
authors.affiliation: Faculty of Veterinary Science, Department of Biological Safety, Kazakh National Agrarian University (KazNAU), Almaty 050010, Kazakhstan.
authors.forename: Zhanara
authors.initials: Z
authors.lastname: Tulemissova
authors.affiliation: Research Institute for Biological Safety Problems (RIBSP), Science Committee, Ministry of Education and Science of the Republic of Kazakhstan, Zhambylskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
authors.forename: Kaissar
authors.initials: K
authors.lastname: Tabynov
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University (OSU), Wooster, OH 44691, USA.
authors.forename: Santosh
authors.initials: S
authors.lastname: Dhakal
authors.affiliation: Research Institute for Biological Safety Problems (RIBSP), Science Committee, Ministry of Education and Science of the Republic of Kazakhstan, Zhambylskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
authors.forename: Aigul
authors.initials: A
authors.lastname: Samoltyrova
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University (OSU), Wooster, OH 44691, USA.
authors.forename: Gourapura J
authors.initials: GJ
authors.lastname: Renukaradhya
authors.affiliation: Research Institute for Biological Safety Problems (RIBSP), Science Committee, Ministry of Education and Science of the Republic of Kazakhstan, Zhambylskaya oblast, Kordaiskiy rayon, Gvardeiskiy 080409, Kazakhstan.
authors.forename: Muratbay
authors.initials: M
authors.lastname: Mambetaliyev
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000917:Antibody Formation / Q000276:immunology; D000918:Antibody Specificity; D005838:Genotype; D019318:Porcine Reproductive and Respiratory Syndrome / Q000097:blood / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology*; D013552:Swine; D015164:Vaccines, Inactivated; D014765:Viral Vaccines / Q000276:immunology*; D014766:Viremia
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
keywords: Binary ethylenimine; Cross-protection; Killed vaccines; Neutralizing antibodies; PRRSV; Polymeric adjuvant