title: Two-Step Chemoenzymatic Detection of N-Acetylneuraminic Acid-α(2-3)-Galactose Glycans.
abstract: Sialic acids are typically linked α(2-3) or α(2-6) to the galactose that located at the non-reducing terminal end of glycans, playing important but distinct roles in a variety of biological and pathological processes. However, details about their respective roles are still largely unknown due to the lack of an effective analytical technique. Herein, a two-step chemoenzymatic approach for the rapid and sensitive detection of N-acetylneuraminic acid-α(2-3)-galactose glycans is described.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Wen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University , Tianjin 300071, China.
authors.forename: Kuan
authors.initials: K
authors.lastname: Jiang
authors.forename: Mingzhen
authors.initials: M
authors.lastname: Zhang
authors.forename: Shukkoor Muhammed
authors.initials: SM
authors.lastname: Kondengaden
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.forename: Kenneth
authors.initials: K
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University , Tianjin 300071, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Song
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University , Tianjin 300071, China.
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D016123:Campylobacter jejuni / Q000201:enzymology; D005690:Galactose / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D057230:Limit of Detection; D017350:N-Acetylgalactosaminyltransferases / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D011134:Polysaccharides; D017350:N-Acetylgalactosaminyltransferases; D019158:N-Acetylneuraminic Acid; D005690:Galactose