title: Pterostilbene as treatment for severe acute pancreatitis.
abstract: Acute pancreatitis (AP) has a fast onset and progression, which lead to an unfavorable prognosis. Therefore, the development of novel drugs for its treatment is critical. As a homologous derivative of resveratrol, pterostilbene exerts a variety of effects including anti-inflammatory, antioxidant, and antitumor effects. This study investigated the potential of pterostilbene for treatment of severe AP (SAP) and related mechanisms. Effects of pterostilbene were evaluated in a Wistar rat model of AP. Serum levels of amylase (AMY), creatinine (Cr), and alanine aminotransferase (ALT) were quantified. Furthermore, serum levels of tumor necrosis factor (TNF)-a and interleukin (IL)-1b were quantified using enzyme-linked immunosorbent assay. Nuclear factor (NF)-kB expression in pancreatic tissues was quantified by real-time PCR and western blotting. The production of reactive oxygen species (ROS) was determined using a spectrometer, while superoxide dismutase (SOD) activity was assayed. In the AP rat model, the expression of inflammatory markers TNF-a and IL-1b, expression of NF-kB, and serum indices (AMY, Cr, and ALT) increased compared to the corresponding levels in the control group (P < 0.05). Pterostilbene reduced serum levels of TNF-a and IL-1b; decreased NF-kB gene expression, serum indices, and ROS generation; and increased SOD activity in a dose-dependent manner. In conclusion, pterostilbene can alleviate SAP-induced tissue damage by decreasing the inflammatory response and by promoting antioxidation leading to the protection of pancreatic tissues.
authors.affiliation: Department of Intensive Care, Sir Run Run Shaw Hospital, Medicine School of Zhejiang University, Hangzhou, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Lin
authors.affiliation: Department of Emergency, Hangzhou Xiasha Hospital, Hangzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Intensive Care, Sir Run Run Shaw Hospital, Medicine School of Zhejiang University, Hangzhou, China.
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.affiliation: Pediatric Intensive Care Unit, Children's Hospital, Medicine School of Zhejiang University, Hangzhou, China ningbotao122@sina.com.
authors.forename: B T
authors.initials: BT
authors.lastname: Ning
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D015415:Biomarkers / Q000097:blood; D004353:Drug Evaluation, Preclinical; D053583:Interleukin-1beta / Q000097:blood; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D019283:Pancreatitis, Acute Necrotizing / Q000097:blood / Q000188:drug therapy*; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D053583:Interleukin-1beta; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; C107773:pterostilbene; D013482:Superoxide Dismutase