title: Kinetic regime of thermal aggregation of holo- and apoglycogen phosphorylases b.
abstract: To characterize the role of pyridoxal 5'-phosphate in stabilization of the conformation of muscle glycogen phosphorylase b (Phb), the mechanism of thermal aggregation for holo- and apoforms of Phb has been studied using dynamic light scattering. The order of aggregation with respect to the protein (n) for aggregation of holoPhb at 48°C is equal to 0.5 suggesting that the dissociative mechanism of denaturation is operative and denaturation is followed by rapid aggregation stage. In the case of aggregation of apoPhb at 37°C n=2 and the rate-limiting stage is aggregation of unfolded protein molecules.
authors.affiliation: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow 119071, Russia. Electronic address: eronina@inbi.ras.ru.
authors.forename: Tatiana B
authors.initials: TB
authors.lastname: Eronina
authors.affiliation: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow 119071, Russia.
authors.forename: Valeriya V
authors.initials: VV
authors.lastname: Mikhaylova
authors.affiliation: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow 119071, Russia.
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Chebotareva
authors.affiliation: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow 119071, Russia.
authors.forename: Boris I
authors.initials: BI
authors.lastname: Kurganov
mesh_terms: D000818:Animals; D001051:Apoenzymes / Q000737:chemistry* / Q000302:isolation & purification; D024982:Glycogen Phosphorylase, Muscle Form / Q000737:chemistry* / Q000302:isolation & purification; D020035:Holoenzymes / Q000737:chemistry* / Q000302:isolation & purification; D006358:Hot Temperature; D007700:Kinetics; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000201:enzymology; D066329:Protein Aggregates; D011487:Protein Conformation; D011489:Protein Denaturation; D055550:Protein Stability; D058767:Protein Unfolding; D011732:Pyridoxal Phosphate / Q000737:chemistry*; D011817:Rabbits; D013816:Thermodynamics
chemical_list: D001051:Apoenzymes; D020035:Holoenzymes; D066329:Protein Aggregates; D011732:Pyridoxal Phosphate; D024982:Glycogen Phosphorylase, Muscle Form
keywords: Glycogen phosphorylase b; Holo- and apoforms; Kinetic regime; Thermal aggregation