title: Metabonomic analysis of biochemical changes in the plasma and urine of carrageenan-induced rats after treatment with Yi-Guan-Jian decoction.
abstract: The urinary and plasma metabonomics method based on a Agilent-1200 LC system coupled to an Agilent-6410 mass spectrometry (HPLC-MS/MS) had been established to investigate the anti-inflammatory activity of Yi-Guan-Jian (YGJ) decoction and explore its potential anti-inflammatory mechanism. Rat acute inflammation was induced by subcutaneous injection of carrageenan in hind paws. Multivariate statistical approaches, such as principal component analysis (PCA), partial least-squares discriminant analysis (PLS-DA) and XCMS online software were used to distinguish normal control group (NG), model group (MG), aspirin-treated group (AG) and Yi-Guan-Jian decoction group (YGJ), aimed at finding out the potential biomarkers. There was a clear separation among the four groups in PCA model. Twenty-five potential biomarkers had been identified using PCA, PLS-DA and XCMS online software. Lastly, we had an enrichment for the related metabolic pathways and screened out the pathways that influence the organism a lot in MetPA, then five mainly metabolism: tryptophan metabolism, lipid metabolism, oxidative stress, glyoxylate and dicarboxylate metabolism, taurine and hypotaurine metabolism were found. In this study, YGJ showed good anti-inflammatory effects and it could suppress the changes of pathologic inflammatory cytokines of carrageenan-induced rat paw edema (CIE). There might be a correlation between these results and the regulation of the disturbed metabolites in urine and plasma. This study demonstrates that metabonomics is a powerful methodology to gain insight into the mechanisms of traditional Chinese medicine (TCM) formula in therapy.
authors.affiliation: Anhui Medical University, Hefei 230032, China; Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China. Electronic address: shuisufang@163.com.
authors.forename: Sufang
authors.initials: S
authors.lastname: Shui
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Shujie
authors.initials: S
authors.lastname: Shen
authors.affiliation: Anhui Medical University, Hefei 230032, China; Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China. Electronic address: rqhuang@aliyun.com.
authors.forename: Rongqing
authors.initials: R
authors.lastname: Huang
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Bingkun
authors.initials: B
authors.lastname: Xiao
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood* / Q000652:urine*; D002351:Carrageenan / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D057230:Limit of Detection; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal; C539461:Yi Guan Jian Decoction; D002351:Carrageenan
keywords: Carrageenin-induced rat paw edema; HPLC–MS/MS; Metabonomics; Traditional Chinese medicine; Yi-Guan-Jian decoction