title: An efficient method for the application of PHA-poor solvents to extract polyhydroxybutyrate from Cupriavidus necator.
abstract: There are many published studies presenting ethanol and acetone as PHAs-poor solvents, where these two solvents are shown to dissolve <2% (w/v) of PHAs at low temperatures. In this study, the suitability of ethanol and acetone for the recovery of PHB at different temperatures (from room temperature to near boiling point) in Cupriavidus necator was investigated. Experiments were performed using response surface methodology to examine the effects of different temperatures and heating incubation times on recovery percentage using the two solvents. The highest recovery percentage (92.3%) and product purity (up to 99%) were obtained with ethanol-assisted extraction at 76°C for 32 min of incubation time. Under these conditions the extracted PHB exhibited a molecular mass of 1.2 × 106. The present strategy showed that at temperatures near its boiling point, ethanol, as a nonhalogenated solvent, represents a good alternative to halogenated solvents, like chloroform, when PHB recovery is concerned. DSC analysis showed good thermal properties for ethanol- and acetone-extracted biopolymers. GC and1H NMR analysis confirmed the extracted biopolymer to be polyhydroxybutyrate of good purity. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1480-1486, 2016.
authors.affiliation: Dept. of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran, Iran.
authors.forename: Asieh
authors.initials: A
authors.lastname: Aramvash
authors.affiliation: Dept. of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Gholami-Banadkuki
authors.affiliation: Dept. of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran, Iran.
authors.forename: Mansooreh-Sadat
authors.initials: MS
authors.lastname: Seyedkarimi
mesh_terms: D000096:Acetone / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D020118:Cupriavidus necator / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry*; D006885:Hydroxybutyrates / Q000737:chemistry / Q000302:isolation & purification*; D054813:Polyhydroxyalkanoates / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D012997:Solvents / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature
chemical_list: D006885:Hydroxybutyrates; D054813:Polyhydroxyalkanoates; D012997:Solvents; D000096:Acetone; D000431:Ethanol
keywords: acetone; central composite design; ethanol; polyhydroxybutyrate; recovery