title: Protein engineering approaches to chemical biotechnology.
abstract: Protein engineering for the improvement of properties of biocatalysts and for the generation of novel metabolic pathways plays more and more important roles in chemical biotechnology aiming at the production of chemicals from biomass. Although widely used in single-enzyme catalysis process, protein engineering is only being increasingly explored in recent years to achieve more complex in vitro and in vivo biocatalytic processes. This review focuses on major contributions of protein engineering to chemical biotechnology in the field of multi-enzymatic cascade catalysis and metabolic engineering. Especially, we discuss and highlight recent strategies for combining pathway design and protein engineering for the production of novel products.
authors.affiliation: Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Denickestrasse 15, D-21073 Hamburg, Germany. Electronic address: aze@TUHH.de.
authors.forename: An-Ping
authors.initials: AP
authors.lastname: Zeng
mesh_terms: D000818:Animals; D055162:Biocatalysis; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D006801:Humans; D060847:Metabolic Engineering / Q000379:methods; D053858:Metabolic Networks and Pathways; D015202:Protein Engineering / Q000379:methods*