title: Immunosuppressive effect of extracts from leaves of Fraxinus Mandshurica Rupr.
abstract: Plants provide a rich resource of medicinal material for research and development of new medicine. To discover new compounds as Immunosuppressant from plants, we evaluated the immunosuppressive effect of different fractions and particularly one compound (Calceolarioside A) that were extracted from the leaves of Fraxinus Mandshurica Rupr. The fractions and the compound were tested on the ability to reduce Immunoglobulin E (IgE) secretion by human U266 multiple myeloma cells (U266 cells) and to reduce interleukin-2 (IL-2) secretion by mouse spleen cells. Our results showed that both the butanol extract fraction and the compound of Calceolarioside A inhibited the IgE and IL-2 production in U266 cells and mouse spleen cells respectively, and no cytotoxicity was observed within the effective dose range. These results suggest that Calceolarioside A could potentially serve as an immunosuppressant.
authors.affiliation: a School of Life Science and Technology , Changchun University of Science and Technology , Changchun , China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: a School of Life Science and Technology , Changchun University of Science and Technology , Changchun , China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xue
authors.affiliation: b Changchun Changsheng Life Sciences Limited , Changchun , China.
authors.forename: Feizhou
authors.initials: F
authors.lastname: Liu
authors.affiliation: c Shanghai Zijiang Enterprise Group Co., LTD, Changchun Branch , Changchun , China.
authors.forename: Xiuling
authors.initials: X
authors.lastname: Gong
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000008:administration & dosage*; D002460:Cell Line; D016207:Cytokines / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D031661:Fraxinus / Q000737:chemistry* / Q000145:classification; D005960:Glucosides / Q000008:administration & dosage*; D006801:Humans; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
chemical_list: D002109:Caffeic Acids; D016207:Cytokines; D005960:Glucosides; D007166:Immunosuppressive Agents; D010936:Plant Extracts; C083066:calceolarioside A
keywords: Calceolarioside A; Fraxinus Mandshurica Rupr; Immunosuppresive effect; U266 cells; spleen cells