title: Development of polymorphic microsatellite markers based on expressed sequence tags in Populus cathayana (Salicaceae).
abstract: Populus cathayana occupies a large area within the northern, central, and southwestern regions of China, and is considered to be an important reforestation species in western China. In order to investigate the population genetic structure of this species, 10 polymorphic microsatellite loci were identified based on expressed sequence tags from de novo sequencing on the Illumina HiSeq 2000 platform. All microsatellite primers were tested on 48 P. cathayana individuals from four locations on the Qinghai-Tibet Plateau. The observed heterozygosity ranged from 0.000 to 1.000, and the null-allele frequency ranged from 0.000 to 0.904. These microsatellite markers may be a useful tool in genetic studies on P. cathayana and closely related species.
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Tian
authors.affiliation: Key Laboratory of Crop Molecular Breeding, Qinghai Province, Xining, China fqzhang@nwipb.cas.cn.
authors.forename: F Q
authors.initials: FQ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Cai
authors.affiliation: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Chen
mesh_terms: D020224:Expressed Sequence Tags; D005787:Gene Frequency; D005819:Genetic Markers; D006579:Heterozygote; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D032107:Populus / Q000235:genetics*
chemical_list: D005819:Genetic Markers