title: Molecular Determinants of Tubulin's C-Terminal Tail Conformational Ensemble.
abstract: Tubulin is important for a wide variety of cellular processes including cell division, ciliogenesis, and intracellular trafficking. To perform these diverse functions, tubulin is regulated by post-translational modifications (PTM), primarily at the C-terminal tails of both the α- and β-tubulin heterodimer subunits. The tubulin C-terminal tails are disordered segments that are predicted to extend from the ordered tubulin body and may regulate both intrinsic properties of microtubules and the binding of microtubule associated proteins (MAP). It is not understood how either interactions with the ordered tubulin body or PTM affect tubulin's C-terminal tails. To probe these questions, we developed a method to isotopically label tubulin for C-terminal tail structural studies by NMR. The conformational changes of the tubulin tails as a result of both proximity to the ordered tubulin body and modification by mono- and polyglycine PTM were determined. The C-terminal tails of the tubulin dimer are fully disordered and, in contrast with prior simulation predictions, exhibit a propensity for β-sheet conformations. The C-terminal tails display significant chemical shift differences as compared to isolated peptides of the same sequence, indicating that the tubulin C-terminal tails interact with the ordered tubulin body. Although mono- and polyglycylation affect the chemical shift of adjacent residues, the conformation of the C-terminal tail appears insensitive to the length of polyglycine chains. Our studies provide important insights into how the essential disordered domains of tubulin function.
authors.affiliation: University of Colorado , Boulder, Colorado, United States.
authors.forename: Kathryn P
authors.initials: KP
authors.lastname: Wall
authors.affiliation: University of Colorado , Boulder, Colorado, United States.
authors.forename: Maria
authors.initials: M
authors.lastname: Pagratis
authors.affiliation: University of Colorado , Boulder, Colorado, United States.
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Armstrong
authors.affiliation: University of Colorado , Boulder, Colorado, United States.
authors.forename: Jeremy L
authors.initials: JL
authors.lastname: Balsbaugh
authors.affiliation: University of Colorado , Boulder, Colorado, United States.
authors.forename: Eric
authors.initials: E
authors.lastname: Verbeke
authors.affiliation: University of Colorado , Anschutz Medical Campus, Colorado, United States.
authors.forename: Chad G
authors.initials: CG
authors.lastname: Pearson
authors.affiliation: University of Colorado , Boulder, Colorado, United States.
authors.forename: Loren E
authors.initials: LE
authors.lastname: Hough
mesh_terms: D000818:Animals; D009682:Magnetic Resonance Spectroscopy; D011487:Protein Conformation; D014404:Tubulin / Q000737:chemistry*
chemical_list: D014404:Tubulin