title: Effects of 5-Fluorouracil, Etoposide and CdCl2in Aquatic OligochaetaLimnodrilus udekemianusClaparede (Tubificidae) Measured by Comet Assay.
abstract: Genotoxicity of 5-fluorouracil (5-FU), etoposide (ET) and cadmium chloride (CdCl2) was evaluated inLimnodrilus udekemianus, cosmopolitan tubificid species, by alkaline single-cell gel electrophoresis (comet assay). Groups of 50 individuals were exposed in vivo in water-only short-term (96 h) tests to 5-FU (0.004, 0.04, 0.4, 4 and 40 μM), ET (0.004. 0.04, 0.4 and 4 μM) and CdCl2(0.004, 0.04, 0.4, 4 and 40 μM). Mortality of worms was observed only for CdCl2(4 and 40 μM). Cell viability lower than 70 % was detected for 5-FU (0.4, 4 and 40 μM), ET (4 μM) and CdCl2(0.4 and 4 μM). All tested substances induced significant increase of DNA damage except 0.004 μM of ET.L. udekemianusbeing sensitive to all tested substances indicates that it can be used in ecogenotoxicology studies. Concern should be raised to cytostatics, especially to 5-FU, since concentration of 0.004 μM induced DNA damage is similar to ones detected in wastewaters.
authors.affiliation: Institute for Biological Research "Siniša Stanković", University of Belgrade, 142 Despota Stefana, 11060 Belgrade, Serbia.
authors.forename: Margareta
authors.initials: M
authors.lastname: Kračun-Kolarević
authors.affiliation: Center for Genotoxicology and Ecogenotoxicology, Chair of Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.
authors.forename: Stoimir
authors.initials: S
authors.lastname: Kolarević
authors.affiliation: Institute for Biological Research "Siniša Stanković", University of Belgrade, 142 Despota Stefana, 11060 Belgrade, Serbia.
authors.forename: Ana
authors.initials: A
authors.lastname: Atanacković
authors.affiliation: Institute for Biological Research "Siniša Stanković", University of Belgrade, 142 Despota Stefana, 11060 Belgrade, Serbia.
authors.forename: Vanja
authors.initials: V
authors.lastname: Marković
authors.affiliation: Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11000 Belgrade, Serbia.
authors.forename: Zoran
authors.initials: Z
authors.lastname: Gačić
authors.affiliation: Institute for Biological Research "Siniša Stanković", University of Belgrade, 142 Despota Stefana, 11060 Belgrade, Serbia.
authors.forename: Momir
authors.initials: M
authors.lastname: Paunović
authors.affiliation: Center for Genotoxicology and Ecogenotoxicology, Chair of Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.
authors.forename: Branka
authors.initials: B
authors.lastname: Vuković-Gačić
keywords: 5-Fluorouracil; CdCl2; Comet assay; Etoposide; Genotoxicity; Limnodrilus udekemianus