title: High sensitive and selective electrochemical biosensor: Label-free detection of human norovirus using affinity peptide as molecular binder.
abstract: Norovirus is known as the major cause of highly infection for gastrointestinal tracts. In this study, robust and highly sensitive biosensors for detecting human norovirus by employing a recognition affinity peptide-based electrochemical platform were described. A series of amino acid-substituted and cysteine-incorporated recognition peptides isolated from evolutionary phage display technique was chemically synthesized and immobilized to a gold sensor layer, the detection performance of the gold-immobilized synthetic peptide-based sensor system was assessed using QCM, CV and EIS. Using EIS, the limit of detection with Noro-1 as a molecular binder was found to be 99.8nM for recombinant noroviral capsid proteins (rP2) and 7.8copies/mL for human norovirus, thereby demonstrating a high degree of sensitivity for their corresponding targets. These results suggest that a biosensor which consists of affinity peptides as a molecular binder and miniaturized microdevices as diagnostic tool could be served as a new type of biosensing platform for point-of-care testing.
authors.affiliation: Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Hwang
authors.affiliation: Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Myung Yi
authors.initials: MY
authors.lastname: Ryu
authors.affiliation: Department of Chemistry, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Chan Young
authors.initials: CY
authors.lastname: Park
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 34141, Republic of Korea.
authors.forename: Junki
authors.initials: J
authors.lastname: Ahn
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 34141, Republic of Korea.
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Park
authors.affiliation: Department of Food and Nutrition, School of Food Science and Technology, Chung-Ang University, Ansung 17546, Republic of Korea.
authors.forename: Changsun
authors.initials: C
authors.lastname: Choi
authors.affiliation: Department of Food Science and Technology, School of Food Science and Technology, Chung-Ang University, Ansung 17546, Republic of Korea.
authors.forename: Sang-Do
authors.initials: SD
authors.lastname: Ha
authors.affiliation: Department of Chemistry, Chung-Ang University, Seoul 06974, Republic of Korea. Electronic address: tjpark@cau.ac.kr.
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Park
authors.affiliation: Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan 38610, Republic of Korea. Electronic address: jppark@dhu.ac.kr.
authors.forename: Jong Pil
authors.initials: JP
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D017250:Caliciviridae Infections / Q000097:blood / Q000175:diagnosis / Q000821:virology*; D002417:Cattle; D003545:Cysteine / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008958:Models, Molecular; D029322:Norovirus / Q000302:isolation & purification*; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D000067716:Point-of-Care Testing
chemical_list: D019151:Peptide Library; D010455:Peptides; D006046:Gold; D003545:Cysteine
keywords: Affinity peptide; Biosensor; Human norovirus; Limit of detection; Molecular binder; Sensitivity