title: Facile Fabrication of PEGylated Fluorescent Organic Nanoparticles with Aggregation-Induced Emission Feature via Formation of Dynamic Bonds and Their Biological Imaging Applications.
abstract: Driven by the high demand for sensitive and specific tools for optical imaging, fluorescent nanoprobes with various working mechanisms and advanced functionalities are flourishing at an incredible speed. This work reports the design and fabrication of aggregation-induced emission (AIE)-active fluorescent organic nanoparticles (FNPs) via forming dynamic phenyl borate between diol containing hydrophobic AIE dye (APD-PhCHO) and phenylboronic acid pendant hydrophilic polymers (PEGMA-VPBA) within 30 min. The final AIE-active APD-PhCHO-PEGMA-VPBA FNPs display high water dispersibility and strong fluorescence emission because of their amphiphilic properties and AIE feature. Biological evaluation suggests that APD-PhCHO-PEGMA-VPBA FNPs possess negative effect on HeLa cells and desirable optical properties for biological imaging. More importantly, phenyl borate is a dynamic bond with pH and glucose responsiveness. Furthermore, different functions can be designed and introduced into these AIE-active systems through adoption of different monomers for good applicability of free radical polymerization. Therefore, this work provides a novel platform for preparation of multifunctional AIE-active nanosystems with responsiveness for various biomedical applications.
authors.affiliation: College of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Long
authors.affiliation: College of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wan
authors.affiliation: College of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China.
authors.forename: Liucheng
authors.initials: L
authors.lastname: Mao
authors.affiliation: College of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China.
authors.forename: Hongye
authors.initials: H
authors.lastname: Huang
authors.affiliation: College of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China.
authors.forename: Guangjian
authors.initials: G
authors.lastname: Zeng
authors.affiliation: College of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Wan
authors.affiliation: College of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China. fengjiedeng@aliyun.com.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Deng
authors.affiliation: College of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang, 330031, China. xiaoyongzhang1980@gmail.com.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and the Tsinghua, Center for Frontier Polymer Research, Tsinghua University, Beijing, 100084, China. weiyen@tsinghua.edu.cn.
authors.forename: Yen
authors.initials: Y
authors.lastname: Wei
mesh_terms: D002470:Cell Survival; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D013501:Surface-Active Agents; D011092:Polyethylene Glycols
keywords: aggregation-induced emissions; block copolymers; core-shell polymers; dynamic bonds; imaging