title: Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase.
abstract: Crop improvement is a never ending process. With a transgenesis approach, it is not inconceivable to envision a continuous addition of new transgenes to existing cultivars. Previously, we described a recombinase-directed gene stacking method in tobacco (Hou et al., Mol Plant 7:1756-1765, 2014). Being able to stack DNA to a previous location ensures that the number of genetic loci does not increase with each new round of transgene addition. Whereas the previous demonstration was conducted through polyethylene glycol to mediate uptake of DNA into tobacco protoplasts, we now describe protocols for using biolistic transformation to stack DNA in tobacco and rice.
authors.affiliation: Plant Gene Engineering Center, South China Botanical Garden, Chinese Academy of Sciences, 723 Xingke Road, Guangzhou, 510650, China.
authors.forename: Ruyu
authors.initials: R
authors.lastname: Li
authors.affiliation: Plant Gene Engineering Center, South China Botanical Garden, Chinese Academy of Sciences, 723 Xingke Road, Guangzhou, 510650, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Han
authors.affiliation: Plant Gene Engineering Center, South China Botanical Garden, Chinese Academy of Sciences, 723 Xingke Road, Guangzhou, 510650, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Hou
authors.affiliation: Plant Gene Engineering Center, South China Botanical Garden, Chinese Academy of Sciences, 723 Xingke Road, Guangzhou, 510650, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Gurminder
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Plant Gene Engineering Center, South China Botanical Garden, Chinese Academy of Sciences, 723 Xingke Road, Guangzhou, 510650, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yin
authors.affiliation: Plant Gene Engineering Center, South China Botanical Garden, Chinese Academy of Sciences, 723 Xingke Road, Guangzhou, 510650, China. dow@scbg.ac.cn.
authors.forename: David W
authors.initials: DW
authors.lastname: Ow
mesh_terms: D019470:Biolistics / Q000379:methods*; D019426:Integrases / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D014026:Nicotiana / Q000235:genetics*; D019076:Transgenes
chemical_list: D019426:Integrases
keywords: Gene stacking; Gene targeting; Recombinase; Site-specific recombination; Transformation; Transgene expression