title: Optical Brain Imaging: A Powerful Tool for Neuroscience.
abstract: As the control center of organisms, the brain remains little understood due to its complexity. Taking advantage of imaging methods, scientists have found an accessible approach to unraveling the mystery of neuroscience. Among these methods, optical imaging techniques are widely used due to their high molecular specificity and single-molecule sensitivity. Here, we overview several optical imaging techniques in neuroscience of recent years, including brain clearing, the micro-optical sectioning tomography system, and deep tissue imaging.
authors.affiliation: Institute of Neuroscience, Department of Neurobiology, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, Zhejiang Province Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, 310058, China.
authors.forename: Xinpei
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Institute of Neuroscience, Department of Neurobiology, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, Zhejiang Province Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, 310058, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Institute of Neuroscience, Department of Neurobiology, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, Zhejiang Province Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, 310058, China.
authors.forename: Xuecen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Neuroscience, Department of Neurobiology, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, Zhejiang Province Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, 310058, China.|State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering and the Collaborative Innovation Center for Brain Science, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Si
authors.affiliation: Institute of Neuroscience, Department of Neurobiology, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, Zhejiang Province Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, 310058, China. weigong@zju.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Gong
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging*; D006801:Humans; D059906:Neuroimaging; D009488:Neurosciences / Q000295:instrumentation / Q000379:methods
keywords: Brain imaging; Deep tissue imaging; MOST; Optical microscopy; Tissue clearing