title: Overexpression of a Chitinase Gene from Trichoderma asperellum Increases Disease Resistance in Transgenic Soybean.
abstract: In the present study, a chi gene from Trichoderma asperellum, designated Tachi, was cloned and functionally characterized in soybean. Firstly, the effects of sodium thiosulfate on soybean Agrobacterium-mediated genetic transformation with embryonic tip regeneration system were investigated. The transformation frequency was improved by adding sodium thiosulfate in co-culture medium for three soybean genotypes. Transgenic soybean plants with constitutive expression of Tachi showed increased resistance to Sclerotinia sclerotiorum compared to WT plants. Meanwhile, overexpression of Tachi in soybean exhibited increased reactive oxygen species (ROS) level as well as peroxidase (POD) and catalase (SOD) activities, decreased malondialdehyde (MDA) content, along with diminished electrolytic leakage rate after S. sclerotiorum inoculation. These results suggest that Tachi can improve disease resistance in plants by enhancing ROS accumulation and activities of ROS scavenging enzymes and then diminishing cell death. Therefore, Tachi represents a candidate gene with potential application for increasing disease resistance in plants.
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, China. 20101040@zknu.edu.cn.
authors.forename: Fuli
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, China.
authors.forename: Xianle
authors.initials: X
authors.lastname: Ruan
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Forestry, Northeast Forestry University, Harbin, 150040, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, China.
authors.forename: Lizong
authors.initials: L
authors.lastname: Hu
authors.affiliation: Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, China. lichengwei@hisp.edu.cn.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Li
mesh_terms: D060054:Agrobacterium / Q000378:metabolism; D000595:Amino Acid Sequence; D001203:Ascomycota / Q000187:drug effects / Q000502:physiology; D016923:Cell Death / Q000187:drug effects; D002688:Chitinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D060467:Disease Resistance / Q000187:drug effects / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D010544:Peroxidases / Q000378:metabolism; D010802:Phylogeny; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000166:cytology / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D013482:Superoxide Dismutase / Q000378:metabolism; D013885:Thiosulfates / Q000494:pharmacology; D014170:Transformation, Genetic / Q000187:drug effects; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D017382:Reactive Oxygen Species; D013885:Thiosulfates; D008315:Malondialdehyde; D010544:Peroxidases; D013482:Superoxide Dismutase; D002688:Chitinases; C017717:sodium thiosulfate
keywords: Overexpression; Physiology; Sclerotinia sclerotiorum resistance; Soybean transformation; Tachi