title: Characterization of a Cyanobacterial Haloperoxidase and Evaluation of its Biocatalytic Halogenation Potential.
abstract: Vanadium-dependent haloperoxidases (VHPOs) are a class of halogenating enzymes found in fungi, lichen, algae, and bacteria. We report the cloning, purification, and characterization of a functional VHPO from the cyanobacterium Acaryochloris marina (AmVHPO), including its structure determination by X-ray crystallography. Compared to other VHPOs, the AmVHPO features a unique set of disulfide bonds that stabilize the dodecameric assembly of the protein. Easy access by high-yield recombinant expression, as well as resistance towards organic solvents and temperature, together with a distinct halogenation reactivity, make this enzyme a promising starting point for the development of biocatalytic transformations.
authors.affiliation: Department Chemie, Center for Integrated Protein Science at the Department Chemie and Catalysis Research Center (CRC), Technische Universität München, Lichtenbergstrasse 4, 85747, Garching, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Frank
authors.affiliation: Department Chemie, Center for Integrated Protein Science at the Department Chemie and Catalysis Research Center (CRC), Technische Universität München, Lichtenbergstrasse 4, 85747, Garching, Germany.
authors.forename: Catharina Julia
authors.initials: CJ
authors.lastname: Seel
authors.affiliation: Department Chemie, Center for Integrated Protein Science at the Department Chemie and Catalysis Research Center (CRC), Technische Universität München, Lichtenbergstrasse 4, 85747, Garching, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Groll
authors.affiliation: Department Chemie, Center for Integrated Protein Science at the Department Chemie and Catalysis Research Center (CRC), Technische Universität München, Lichtenbergstrasse 4, 85747, Garching, Germany. tanja.gulder@tum.de.
authors.forename: Tanja
authors.initials: T
authors.lastname: Gulder
mesh_terms: D055162:Biocatalysis; D018360:Crystallography, X-Ray; D000458:Cyanobacteria / Q000201:enzymology*; D054879:Halogenation; D008958:Models, Molecular; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010544:Peroxidases
keywords: enzyme catalysis; halogenation; peroxidases; protein structures; vanadium