title: High Expression of Vimentin is Associated With Progression and a Poor Outcome in Glioblastoma.
abstract: Glioblastoma multiforme (GBM) has a high recurrence and mortality rate. Because of a poor understanding of the mechanism for this disease, treatment regimens have remained limited. Vimentin, one of the major cytoskeletal proteins, is associated with cellular structure. However, the function of vimentin in GBM is still undefined. In the present study, we investigated the expression level of vimentin in 179 GBM tissues using immunohistochemistry. We found that the vimentin expression level was associated with the time to progression (P=0.029). A Kaplan-Meier analysis revealed that patients with high vimentin expression had a significantly shorter overall survival (P=0.0002) and progression-free survival (P=0.0001) compared with those with low expression. Furthermore, in vitro experiments showed that withaferin-A, a chemical inhibitor of vimentin, could inhibit GBM cell migration and invasion activity when its concentrations were <0.5 μM, and higher concentrations of withaferin-A could decrease the viability of U251and U87 cells significantly. In conclusion, our results indicated that vimentin may play an important role in the progression of GBM.
authors.affiliation: Department of Neurosurgery, The Fourth Affiliated Hospital of Harbin Medical University.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Departments of Parasitology.
authors.forename: Liqiu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Biochemistry and Molecular Biology.
authors.forename: Xingli
authors.initials: X
authors.lastname: Dong
authors.affiliation: Pharmacology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Pharmacology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Linman
authors.initials: L
authors.lastname: Huo
authors.affiliation: Department of Neurosurgery, The Fourth Affiliated Hospital of Harbin Medical University.
authors.forename: Huirong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000401:mortality; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D002465:Cell Movement; D003599:Cytoskeleton / Q000378:metabolism; D005260:Female; D005909:Glioblastoma / Q000175:diagnosis* / Q000401:mortality; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local; D016019:Survival Analysis; D014746:Vimentin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054358:Withanolides / Q000494:pharmacology
chemical_list: D014746:Vimentin; D054358:Withanolides; C009684:withaferin A