title: HCVIVdb: The hepatitis-C IRES variation database.
abstract: Sequence variability in the hepatitis C virus (HCV) genome has led to the development and classification of six genotypes and a number of subtypes. The HCV 5' untranslated region mainly comprises an internal ribosomal entry site (IRES) responsible for cap-independent synthesis of the viral polyprotein and is conserved among all HCV genotypes.
  
  
  
  Considering the possible high impact of variations in HCV IRES on viral protein production and thus virus replication, we decided to collect the available data on known nucleotide variants in the HCV IRES and their impact on IRES function in translation initiation. The HCV IRES variation database (HCVIVdb) is a collection of naturally occurring and engineered mutation entries for the HCV IRES. Each entry contains contextual information pertaining to the entry such as the HCV genotypic background and links to the original publication. Where available, quantitative data on the IRES efficiency in translation have been collated along with details on the reporter system used to generate the data. Data are displayed both in a tabular and graphical formats and allow direct comparison of results from different experiments. Together the data provide a central resource for researchers in the IRES and hepatitis C-oriented fields.
  
  
  
  The collation of over 1900 mutations enables systematic analysis of the HCV IRES. The database is mainly dedicated to detailed comparative and functional analysis of all the HCV IRES domains, which can further lead to the development of site-specific drug designs and provide a guide for future experiments. HCVIVdb is available at http://www.hcvivdb.org .
authors.affiliation: Department of Genetics & Microbiology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44, Prague 2, Czech Republic.|Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona, Spain.|Universitat Pompeu Fabra (UPF), Barcelona, Spain.
authors.forename: Evan W
authors.initials: EW
authors.lastname: Floden
authors.affiliation: Department of Genetics & Microbiology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44, Prague 2, Czech Republic.
authors.forename: Anas
authors.initials: A
authors.lastname: Khawaja
authors.affiliation: Department of Genetics & Microbiology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44, Prague 2, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Vopálenský
authors.affiliation: Department of Genetics & Microbiology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44, Prague 2, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Pospíšek
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D003625:Data Collection; D030541:Databases, Genetic; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics* / Q000378:metabolism; D006526:Hepatitis C / Q000821:virology; D006801:Humans; D000067556:Internal Ribosome Entry Sites / Q000235:genetics*; D009154:Mutation; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D000067556:Internal Ribosome Entry Sites; D012367:RNA, Viral; D014764:Viral Proteins
keywords: Database; HCV; Hepatitis C; IRES; Internal ribosome entry site; Translation efficiency