title: Anthelmintic activity of acetone extracts from South African plants used on egg hatching of <I>Haemonchus contortus</I>.
abstract: The nematode, Haemonchus contortus, is responsible for major economic losses in the livestock industry. The management of parasites such as H. contortus has been through the use of synthetic parasiticides. This has resulted in the presence of residues in meat and milk, which affects food safety. The development of resistance to available anthelmintics coupled with their high cost has further complicated matters. This has led to the investigation of alternative methods to manage nematodes, including the use of plants and plant extracts as a potential source of novel anthelmintics. Acetone extracts were prepared from 15 South African plant species and their anthelmintic activity determined using the egg hatch assay (EHA). The leaf extract of Cleome gynandra had the best inhibitory activity (68% ± 3%) at a concentration of 2.5 mg/mL, followed by the stem extract of Maerua angolensis (65% ± 5%). The extracts had a relatively low toxicity on Vero cells determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) cellular assay.
authors.forename: Gerda
authors.initials: G
authors.lastname: Fouche
authors.forename: Bellonah M
authors.initials: BM
authors.lastname: Sakong
authors.forename: Olubukola T
authors.initials: OT
authors.lastname: Adenubi
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Pauw
authors.forename: Tlabo
authors.initials: T
authors.lastname: Leboho
authors.affiliation: CSIR Biosciences, Pretoria. kwellington@csir.co.za.
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Wellington
authors.forename: Jacobus N
authors.initials: JN
authors.lastname: Eloff
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D006188:Haemonchiasis / Q000517:prevention & control / Q000662:veterinary*; D006189:Haemonchus / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D058751:Livestock; D010063:Ovum / Q000187:drug effects; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts