title: Overexpression of OsMYB1R1-VP64 fusion protein increases grain yield in rice by delaying flowering time.
abstract: VP64 is widely used as a transcriptional activator to investigate the biological function of genes, but its potential for application in genetic improvement of crops has not been fully investigated. Here, we characterized an OsMYB1R1-VP64 fusion protein that enhanced the grain yield of rice cultivar 'Kita-ake' by 35%. OsMYB1R1-VP64 regulated grain yield of transgenic plants mainly by extending the vegetative growth stage. Further analysis indicated that OsMYB1R1-VP64 delayed flowering by down-regulating expression of Heading date 3a (Hd3a) and RICE FLOWERING LOCUST 1 (RFT1) through repression of Early heading date 1 (Ehd1). The expression pattern of OsMYB1R1 was constitutive and rhythmically controlled. OsMYB1R1 protein had transcriptional activation activity and was localized to the nucleus. Our findings suggest that the OsMYB1R1-VP64 construct can be used to increase grain yield in rice.
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, China.
authors.forename: Jiachang
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Fuqing
authors.initials: F
authors.lastname: Wu
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhu
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Jiulin
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Chaonan
authors.initials: C
authors.lastname: Li
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Peike
authors.initials: P
authors.lastname: Sheng
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, China.
authors.forename: Maohong
authors.initials: M
authors.lastname: Cai
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Guo
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, China.
authors.forename: Chunming
authors.initials: C
authors.lastname: Wang
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, China. wanjm@njau.edu.cn, wanjianmin@caas.cn.|National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China. wanjm@njau.edu.cn, wanjianmin@caas.cn.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wan
mesh_terms: D018556:Crops, Agricultural / Q000235:genetics* / Q000254:growth & development; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation; D019076:Transgenes
chemical_list: D011993:Recombinant Fusion Proteins; D014157:Transcription Factors
keywords: VP64; flowering time; grain yield