title: Identification of the invertase gene family (INVs) in tea plant and their expression analysis under abiotic stress.
abstract: Fourteen invertase genes were identified in the tea plant, all of which were shown to participate in regulating growth and development, as well as in responding to various abiotic stresses. Invertase (INV) can hydrolyze sucrose into glucose and fructose, which plays a principal role in regulating plant growth and development as well as the plants response to various abiotic and biotic stresses. However, currently, there is a lack of reported information, regarding the roles of INVs in either tea plant development or in the tea plants response to various stresses. In this study, 14 INV genes were identified from the transcriptome data of the tea plant (Camellia sinensis (L.) O. Kuntze), and named CsINV1-5 and CsINV7-15. Based on the results of a Blastx search and phylogenetic analysis, the CsINV genes could be clustered into 6 acid invertase (AI) genes and 8 alkaline/neutral invertase (A/N-Inv) genes. The results of tissue-specific expression analysis showed that the transcripts of all the identified CsINV genes are detectable in various tissues. Under various abiotic stress conditions, the expression patterns of the 14 CsINV genes were diverse in both the leaves and roots, and some of them were shown to be significantly expressed. Overall, we hypothesize that the identified CsINV genes all participate in regulating growth and development in the tea plant, and most likely through different signaling pathways that regulate the carbohydrate allocation and the ratio of hexose and sucrose for improving the resistance of the leaves and the roots of the tea plant to various abiotic stresses.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.|Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, 310008, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Qian
authors.affiliation: Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, 310008, China.|Department of Tea Science, College of Horticulture, Fujian A&F University, Fuzhou, 350002, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Yue
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.|Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, 310008, China.
authors.forename: Yuchun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, 310008, China.|Department of Tea Science, College of Horticulture, Fujian A&F University, Fuzhou, 350002, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Cao
authors.affiliation: Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, 310008, China.
authors.forename: Nana
authors.initials: N
authors.lastname: Li
authors.affiliation: Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, 310008, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, 310008, China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Hao
authors.affiliation: Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, 310008, China. xcw75@tricaas.com.
authors.forename: Xinchao
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China. 1647785720@qq.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Xiao
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China. yjyang@tricaas.com.|Tea Research Institute of the Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, 310008, China. yjyang@tricaas.com.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Yang
mesh_terms: D020816:Amino Acid Motifs; D028241:Camellia sinensis / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D017124:Conserved Sequence / Q000235:genetics; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005810:Multigene Family; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D000072417:Protein Domains; D013312:Stress, Physiological / Q000235:genetics*; D013997:Time Factors; D043324:beta-Fructofuranosidase / Q000235:genetics* / Q000378:metabolism
chemical_list: D043324:beta-Fructofuranosidase
keywords: Abiotic stress; Gene expression; Invertase gene; Tea plant (Camellia sinensis)