title: MiR-155 and its functional variant rs767649 contribute to the susceptibility and survival of hepatocellular carcinoma.
abstract: Hepatocellular carcinoma (HCC) ranks the fourth common cancer and the third common cause of cancer mortality among Chinese population. The development of hepatocellular carcinoma (HCC) were confirmed to be involved in complex interactions between environmental and genetic factors. MicroRNAs (miRNAs) have been found to play an important role in tumorigenesis and metastasis. Emerging evidence suggested that upregulation of miR-155, one of the best characterized miRNAs, could serve as a promising marker for the diagnosis and prognosis of many cancers, except for HCC. In current we tested the hypothesis that functional variant rs767649 located in the flanking region of miR-155 gene contributes to the development and survival of HCC. We identified that functional variant rs767649 in miR-155 regulation region was associated with risk and survival of HCC. The minor allele of rs767649 was significantly associated with an increased risk of HCC (OR=1.23, 95% CI=1.11-1.36, P = 7.97x10-5). The genotype TT of rs767649 was significantly associated with a 1.94 fold poor survival of HCC (HR=1.94, 95% CI=1.01-3.79), while 1.15 fold for genotype AT (HR=1.15, 95% CI=1.06-1.25). Results showed that miR-155 was highly overexpressed in HCC tissues, compared with the adjacent normal tissues (P<0.001). The allele T contributes to higher expression of miR-155 in both the HCC tissues and the adjacent non-tumor tissues (P< 0.01). Our findings suggested that miR-155 and its functional variant rs767649 might contribute to the increased risk and poor prognosis of HCC, highlighting the importance of miR-155 in the prevention and prognosis of HCC.
authors.affiliation: Department of Radiology, Affiliated Lishui Hospital of Zhejiang University, The Fifth Affiliated Hospital of Wenzhou Medical University, The Central Hospital of Zhejiang, Zhejiang 323000, P. R. China.
authors.forename: Jiansong
authors.initials: J
authors.lastname: Ji
authors.affiliation: Department of Radiology, Affiliated Lishui Hospital of Zhejiang University, The Fifth Affiliated Hospital of Wenzhou Medical University, The Central Hospital of Zhejiang, Zhejiang 323000, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.affiliation: Department of Radiology, Affiliated Lishui Hospital of Zhejiang University, The Fifth Affiliated Hospital of Wenzhou Medical University, The Central Hospital of Zhejiang, Zhejiang 323000, P. R. China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Tu
authors.affiliation: Department of Radiology, Affiliated Lishui Hospital of Zhejiang University, The Fifth Affiliated Hospital of Wenzhou Medical University, The Central Hospital of Zhejiang, Zhejiang 323000, P. R. China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Hepatobiliary Surgery, Beijing Chao-Yang Hospital Affiliated with Capital Medical University, Beijing 100043, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Radiology, Affiliated Lishui Hospital of Zhejiang University, The Fifth Affiliated Hospital of Wenzhou Medical University, The Central Hospital of Zhejiang, Zhejiang 323000, P. R. China.
authors.forename: Minjiang
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Radiology, Affiliated Lishui Hospital of Zhejiang University, The Fifth Affiliated Hospital of Wenzhou Medical University, The Central Hospital of Zhejiang, Zhejiang 323000, P. R. China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Radiology, Zhong-da Hospital, Medical School, Southeast University, Nanjing 210009, P. R. China.
authors.forename: Haidong
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Radiology, Affiliated Lishui Hospital of Zhejiang University, The Fifth Affiliated Hospital of Wenzhou Medical University, The Central Hospital of Zhejiang, Zhejiang 323000, P. R. China.
authors.forename: Xingyao
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Radiology, Affiliated Lishui Hospital of Zhejiang University, The Fifth Affiliated Hospital of Wenzhou Medical University, The Central Hospital of Zhejiang, Zhejiang 323000, P. R. China.
authors.forename: Junguo
authors.initials: J
authors.lastname: Hui
authors.affiliation: Department of Radiology, Affiliated Lishui Hospital of Zhejiang University, The Fifth Affiliated Hospital of Wenzhou Medical University, The Central Hospital of Zhejiang, Zhejiang 323000, P. R. China.
authors.forename: Xilin
authors.initials: X
authors.lastname: Lan
authors.affiliation: Department of Radiology, Lab-Yang, University of Washington, Seattle, WA 98109, USA.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yang
mesh_terms: D000483:Alleles; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012307:Risk Factors; D016019:Survival Analysis
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs
keywords: miRNA; microRNA-155; susceptibility