title: SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley.
abstract: A high-density linkage map is a valuable tool for functional genomics and breeding. A newly developed sequence-based marker technology, restriction site associated DNA (RAD) sequencing, has been proven to be powerful for the rapid discovery and genotyping of genome-wide single nucleotide polymorphism (SNP) markers and for the high-density genetic map construction. The objective of this research was to construct a high-density genetic map of barley using RAD sequencing. 1894 high-quality SNP markers were developed and mapped onto all seven chromosomes together with 68 SSR markers. These 1962 markers constituted a total genetic length of 1375.8 cM and an average of 0.7 cM between adjacent loci. The number of markers within each linkage group ranged from 209 to 396. The new recessive dwarfing gene btwd1 in Huaai 11 was mapped onto the high density linkage maps. The result showed that the btwd1 is positioned between SNP marks 7HL_6335336 and 7_249275418 with a genetic distance of 0.9 cM and 0.7 cM on chromosome 7H, respectively. The SNP-based high-density genetic map developed and the dwarfing gene btwd1 mapped in this study provide critical information for position cloning of the btwd1 gene and molecular breeding of barley.
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Ren
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jibin
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Lipan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Biology Department, Saint Mary's University, 923 Robie Street, Halifax, NS, B3H3C3, Canada.
authors.forename: Genlou
authors.initials: G
authors.lastname: Sun
authors.affiliation: Department of Agriculture &Food/Agricultural Research Western Australia, 3 Baron-Hay Court, South Perth, WA 6155, Australia.
authors.forename: Chengdao
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Luo
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.|Hubei Collaborative Innovation Center for Grain Industry, Jingzhou, 434025, Hubei, China.
authors.forename: Dongfa
authors.initials: D
authors.lastname: Sun
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D017343:Genes, Plant; D005808:Genes, Recessive; D055106:Genome-Wide Association Study; D001467:Hordeum / Q000235:genetics* / Q000254:growth & development; D020641:Polymorphism, Single Nucleotide