title: The modulation of MiR-155 and MiR-23a manipulates Klebsiella pneumoniae Adhesion on Human pulmonary Epithelial cells via Integrin α5β1 Signaling.
abstract: Micro-RNAs (miRNAs) critically regulate several host defense mechanisms, but their roles in the bacteria-epithelium interplay remain unclear. Our results displayed that the expression of miR-155 and miR-23a were down-regulated in K. pneumoniae-infected pulmonary epithelial cells. The elevated bacterial adhesion on A549 cells followed the enhancement of the cellular levels of these two miRNAs. Meanwhile, a mechanistic study demonstrated that miR-155 promoted integrin α5β1 function and resulted in the increased actin polymerization. Moreover, a non-histone nuclear protein, high mobility group nucleosomal-binding domain 2 (HMGN2) served as the potential target of miR-155 and miR-23a to regulate the integrin α5β1 expression and K. pneumoniae adhesion. Furthermore, the expression of a known integrin transcription suppressor-Nuclear Factor-I (NFI) was also repressed by miR-155, which paralleled with its chromatin location in the promoter regions of integrin α5 and β1. These results uncover novel links between miRNAs and integrin function to regulate bacterial adhesion, indicating a potential mechanism of host cell autonomous immune response to K. pneumoniae infection.
authors.affiliation: Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Teng
authors.affiliation: Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Junming
authors.initials: J
authors.lastname: Miao
authors.affiliation: Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Shen
authors.affiliation: Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Laibin
authors.initials: L
authors.lastname: Ren
authors.affiliation: Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
authors.affiliation: Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Chen
authors.affiliation: Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Li
authors.affiliation: Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Shanze
authors.initials: S
authors.lastname: Chen
authors.affiliation: Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Research Unit of Infection and Immunity, Department of Pathophysiology, West China College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Huang
mesh_terms: D056809:Alveolar Epithelial Cells / Q000378:metabolism*; D001422:Bacterial Adhesion; D002460:Cell Line; D015536:Down-Regulation; D006801:Humans; D039081:Integrin alpha5beta1 / Q000378:metabolism*; D007710:Klebsiella Infections / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D039081:Integrin alpha5beta1; C524942:MIRN155 microRNA, human; C552430:MIRN23a microRNA, human; D035683:MicroRNAs