title: Effect of Kineret® on ovarian ischemia reperfusion injury in a rat model.
abstract: OBJECTIVE
  To investigate the effect of Kineret® on ischemia reperfusion (IR) injury in rat ovaries.
  
  
  METHODS
  Rats were divided into four groups: ovarian IR (IRG); 50 mg/kg Kineret® + ovarian IR (KIR-50); 100 mg/kg Kineret® + ovarian IR (KIR-100); and sham operation (SOC). KIR-50 (n = 10) and KIR-100 (n = 10) groups received an intraperitoneal injection of Kineret® at doses of 50 and 100 mg/kg, respectively. IRG and SOC (n = 10) rat groups were given distilled water as solvent using the same method. The results were compared between the groups.
  
  
  RESULTS
  In rats in which IR occurred, oxidant parameters, such as malondialdehyde (MDA) and myeloperoxidase (MPO), were increased, the level of proinflammatory interleukin 1 beta (IL-1β) was elevated and total glutathione (tGSH) as an antioxidant was decreased in the ovarian tissues. Administration of Kineret® at a dose of 100 mg/kg inhibited the increase of MDA, MOP and IL-1β and a decrease in tGSH caused by IR more significantly than administration of Kineret® at a dose of 50 mg/kg. In addition, 100 mg/kg Kineret® significantly decreased severe hemorrhage, degeneration and inflammatory signs in the follicular cells, caused by IR. Kineret® at 100 mg/kg markedly ameliorated increased apoptosis in ovarian tissue with IR more significantly than 50 mg/kg kineret.
  
  
  CONCLUSIONS
  Our findings indicate that Kineret® might be useful in clinical practice for the treatment of damage that may occur as a result of ovarian torsion.
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, Erzincan University, Erzincan, Turkey.
authors.forename: Umit Arslan
authors.initials: UA
authors.lastname: Nayki
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine, Erzincan University, Erzincan, Turkey.
authors.forename: Cenk
authors.initials: C
authors.lastname: Nayki
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Erzincan University, Erzincan, Turkey. nihalcetin84@gmail.com.
authors.forename: Nihal
authors.initials: N
authors.lastname: Cetin
authors.affiliation: Department of Pathology, Mengucek Gazi Education and Research Hospital, Erzincan, Turkey.
authors.forename: Ferda Keskin
authors.initials: FK
authors.lastname: Cimen
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Erzincan University, Erzincan, Turkey.
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Coban
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Erzincan University, Erzincan, Turkey.
authors.forename: Renad
authors.initials: R
authors.lastname: Mammadov
authors.affiliation: Department of Parasitology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Ismail Hakkı
authors.initials: IH
authors.lastname: Tas
authors.affiliation: Department of Anatomy, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors.forename: Ismail
authors.initials: I
authors.lastname: Malkoc
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D000975:Antioxidants / Q000008:administration & dosage*; D053148:Caspase 3 / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D005978:Glutathione / Q000235:genetics; D053590:Interleukin 1 Receptor Antagonist Protein / Q000008:administration & dosage*; D053583:Interleukin-1beta / Q000235:genetics; D008315:Malondialdehyde / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D009195:Peroxidase / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D008315:Malondialdehyde; D009195:Peroxidase; C505410:Casp3 protein, rat; D053148:Caspase 3; D005978:Glutathione
keywords: IL-1β; apoptosis; ischemia/reperfusion; kineret; ovary; rat