title: The complete genome sequence of PE3-1, a novel E. coli O153 phage.
abstract: A novel virulent phage PE3-1 against E. coli O153 was isolated from an aeration tank in a wastewater treatment plant. Transmission electron microscopy images showed that phage PE3-1 had an icosahedral head and a short tail, which revealed that it was a member of the family Podoviridae of the order Caudovirales. The complete PE3-1 genome consisted of 39,093 bp and was a linear double-stranded DNA with an average GC content of 49.93 %. Phage PE3-1 showed homology to the T7-like phages in 48 open reading frames (ORFs), but it differed from previously reported E .coli phages in morphology and bioinformatics analysis. This indicated that phage PE3-1 is a new member of the genus T7 virus.
authors.affiliation: College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, China.
authors.forename: Honghui
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, China. xcliu@ucas.ac.cn.
authors.forename: Xinchun
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yi
authors.affiliation: College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, China.
authors.forename: Ruyin
authors.initials: R
authors.lastname: Liu
authors.affiliation: College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
mesh_terms: D001482:Base Composition; D019149:Bioreactors / Q000821:virology; D003090:Coliphages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D016679:Genome, Viral; D046529:Microscopy, Electron, Transmission; D017902:Podoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014771:Virion / Q000648:ultrastructure; D062065:Wastewater / Q000821:virology
chemical_list: D004247:DNA; D004279:DNA, Viral; D062065:Wastewater