title: Effects of melatonin on oocyte maturation in PCOS mouse model.
abstract: The purpose of oocyte in vitro maturation is generation of mature oocytes that could support future development. Efforts have been made to enhance oocyte developmental competence by developing optimal culture conditions. The present study is conducted to determine melatonin effects on quality of polycystic ovarian syndrome (PCOS) oocytes when it has been added during in vitro maturation, and immature oocytes were cultured in defined conditioned medium with and without different melatonin concentrations. Melatonin could significantly improve nuclear maturation of PCOS oocytes (81.1% vs. 56.3%, P < 0.05 were achieved with 10-6mol/L concentration). Cleavage rate was significantly higher in 10-5mol/L concentration compared to untreated oocytes in PCOS (54% vs. 35%, respectively) and it was significantly higher with 10-6mol/L concentration in the control group, 55% versus 38%, compared to untreated oocytes. This study showed that melatonin has the potential to induce oocyte nuclear maturation and guarantee fertilization potential. © 2016 Japanese Society of Animal Science.
authors.affiliation: Anatomy Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Nikmard
authors.affiliation: Anatomy Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Hosseini
authors.affiliation: Anatomy Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.|Cellular and Molecular Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Bakhtiyari
authors.affiliation: Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute, ACECR, Tehran, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Ashrafi
authors.affiliation: Anatomy Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Fardin
authors.initials: F
authors.lastname: Amidi
authors.affiliation: Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute, ACECR, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Aflatoonian
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D030762:Estrous Cycle / Q000502:physiology; D005260:Female; D005306:Fertilization / Q000187:drug effects; D005307:Fertilization in Vitro; D008550:Melatonin / Q000494:pharmacology*; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000254:growth & development*; D011085:Polycystic Ovary Syndrome / Q000503:physiopathology*; D013268:Stimulation, Chemical
chemical_list: D008550:Melatonin
keywords: in vitro maturation; melatonin; polycystic ovarian syndrome model