title: Quantitative Analysis of Cancer Metabolism: From pSIRM to MFA.
abstract: Metabolic reprogramming is a required step during oncogenesis and essential for cellular proliferation. It is triggered by activation of oncogenes and loss of tumor suppressor genes. Beside the combinatorial events leading to cancer, common changes within the central metabolism are reported. Increase of glycolysis and subsequent lactic acid formation has been a focus of cancer metabolism research for almost a century. With the improvements of bioanalytical techniques within the last decades, a more detailed analysis of metabolism is possible and recent studies demonstrate a wide range of metabolic rearrangements in various cancer types. However, a systematic and mechanistic understanding is missing thus far. Therefore, analytical and computational tools have to be developed allowing for a dynamic and quantitative analysis of cancer metabolism. In this chapter, we outline the application of pulsed stable isotope resolved metabolomics (pSIRM) and describe the interface toward computational analysis of metabolism.
authors.affiliation: Integrative Proteomics and Metabolomics, Max-Delbrueck-Center of Molecular Medicine in the Helmholtz Association, Robert-Roessle-Str. 10, 13125, Berlin, Germany.
authors.forename: Christin
authors.initials: C
authors.lastname: Zasada
authors.affiliation: Integrative Proteomics and Metabolomics, Max-Delbrueck-Center of Molecular Medicine in the Helmholtz Association, Robert-Roessle-Str. 10, 13125, Berlin, Germany. stefan.kempa@mdc-berlin.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kempa
mesh_terms: D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D055432:Metabolomics / Q000379:methods*; D009369:Neoplasms / Q000378:metabolism*
keywords: Cancer metabolism; Metabolic flux analysis; Metabolomics; Proteomics; Pulsed stable isotope resolved metabolomics (pSIRM); Quantitative mass spectrometry