title: Morphological change of skin fibroblasts induced by UV Irradiation is involved in photoaging.
abstract: Human dermal fibroblasts (HDFs) are typically flattened or extensible shaped and play a critical role in the metabolism of extracellular matrix components. As the properties of fibroblasts in the dermis are considered to be influenced by their morphology, we investigated the morphological changes induced in fibroblasts by ultraviolet (UV) irradiation as well as the relationship between these changes and collagen metabolism. In this study, we showed that UVA exposure induced morphological changes and reduced collagen contents in HDFs. These morphological changes were accompanied a reduction in actin filaments and upregulation of the actin filament polymerization inhibitor, capping protein muscle Z-line ɑ1 (CAPZA1). External actin filament growth inhibitors also affected the shape of HDFs and reduced collagen levels. These results suggest that UVA exposure may inhibit the polymerization of actin filaments and induce morphological changes in skin fibroblasts. These morphological changes in fibroblasts may accelerate reductions in collagen synthesis. This mechanism may be one of the processes responsible for collagen reductions observed in photoaged skin. When natural materials that suppress these morphological changes in HDFs were evaluated, we found that an extract of Lilium 'Casa Blanca' (LCB) suppressed UVA-induced alterations in the shape of HDFs, which are typically followed by inhibition of collagen reduction. An analysis of the active compounds in LCB extract led to the identification of regaloside I, which had a structure of phenylpropanoid glycerol glucoside, as the active compound inhibiting the upregulation of CAPZA1. Therefore, inhibition of UVA-induced morphological changes in HDFs is considered to be promising way for the suppression of collagen reduction in photoaging.
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamaba
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, Japan.
authors.forename: Manami
authors.initials: M
authors.lastname: Haba
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kunita
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sakaida
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, Japan.
authors.forename: Youichi
authors.initials: Y
authors.lastname: Yashiro
authors.affiliation: Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Nakata
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D051347:CapZ Actin Capping Protein / Q000235:genetics / Q000378:metabolism; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D015638:Cytochalasin D / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D027762:Lilium; D010936:Plant Extracts / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects*; D015595:Skin Aging / Q000473:pathology* / Q000502:physiology / Q000528:radiation effects*; D053778:Thiazolidines / Q000494:pharmacology; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; C495842:CAPZA1 protein, human; D051347:CapZ Actin Capping Protein; D010936:Plant Extracts; D012333:RNA, Messenger; D053778:Thiazolidines; D015638:Cytochalasin D; D003094:Collagen; C037068:latrunculin B
keywords: actin filaments; collagen; morphology; photoaging; skin fibroblasts