title: Factors Affecting the Radiosensitivity of Hexaploid Wheat to γ-Irradiation: Radiosensitivity of Hexaploid Wheat (Triticum aestivum L.) [Corrected].
abstract: Understanding the radiosensitivity of plants, an important factor in crop mutation breeding programs, requires a thorough investigation of the factors that contribute to this trait. In this study, we used the highly radiosensitive wheat (Triticum aestivum L.) variety HY1 and J411, a γ-irradiation-insensitive control, which were screened from a natural population, to examine the factors affecting radiosensitivity, including free radical content and total antioxidant capacity, as well as the expression of TaKu70 and TaKu80 (DNA repair-related genes) as measured by real-time PCR. We also investigated the alternative splicing of this gene in the wild-type wheat ecotype by sequence analysis. Free radical contents and total antioxidant capacity significantly increased upon exposure of HY1 wheat to γ-irradiation in a dose-dependent manner. By contrast, in J411, the free radical contents exhibited a similar trend, but the total antioxidant capacity exhibited a downward trend upon increasing γ-irradiation. Additionally, we detected dose-dependent increases in TaKu70 and TaKu80 expression levels in γ-irradiated HY1, while in J411, TaKu70 expression levels increased, followed by a decline. We also detected alternative splicing of TaKu70 mRNA, namely, intron retention, in HY1 but not in J411. Our findings indicate that γ-irradiation induces oxidative stress and DNA damage in hexaploid wheat, resulting in growth retardation of seedlings, and they suggest that TaKu70 may play a causal role in radiosensitivity in HY1. Further studies are required to exploit these factors to improve radiosensitivity in other wheat varieties.
authors.affiliation: Academy of Life Science, Qingdao Agricultural University, Qingdao, China.|Institute of Crop Sciences, Chinese Academy of Agricultural Sciences /National Key Facility for Crop Gene Resources and Genetic Improvement /National Center of Space Mutagenesis for Crop Improvement, Beijing, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences /National Key Facility for Crop Gene Resources and Genetic Improvement /National Center of Space Mutagenesis for Crop Improvement, Beijing, China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Gu
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences /National Key Facility for Crop Gene Resources and Genetic Improvement /National Center of Space Mutagenesis for Crop Improvement, Beijing, China.
authors.forename: Linshu
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences /National Key Facility for Crop Gene Resources and Genetic Improvement /National Center of Space Mutagenesis for Crop Improvement, Beijing, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Guo
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences /National Key Facility for Crop Gene Resources and Genetic Improvement /National Center of Space Mutagenesis for Crop Improvement, Beijing, China.
authors.forename: Yongdun
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences /National Key Facility for Crop Gene Resources and Genetic Improvement /National Center of Space Mutagenesis for Crop Improvement, Beijing, China.
authors.forename: Shirong
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Academy of Life Science, Qingdao Agricultural University, Qingdao, China.
authors.forename: Xiyun
authors.initials: X
authors.lastname: Song
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences /National Key Facility for Crop Gene Resources and Genetic Improvement /National Center of Space Mutagenesis for Crop Improvement, Beijing, China.
authors.forename: Longzhi
authors.initials: L
authors.lastname: Han
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences /National Key Facility for Crop Gene Resources and Genetic Improvement /National Center of Space Mutagenesis for Crop Improvement, Beijing, China.
authors.forename: Luxiang
authors.initials: L
authors.lastname: Liu