title: IL-22 promoted CD3+ T cell infiltration by IL-22R induced STAT3 phosphorylation in murine acute graft versus host disease target organs after allogeneic bone marrow transplantation.
abstract: Graft versus host disease (GVHD) is a life threatening complication of bone marrow stem cell transplantation, in which considerable numbers of proinflammatory cytokines secreted by allo-reactive donor T cells are involved. We and other previous studies have found that interleukin-22 (IL-22) was able to aggravate the target organs damage of GVHD. However, the mechanism and the signal pathway of IL-22 in murine acute GVHD was not clear. Here, we observed that compared with GVHD group, more serious pathological damage and more CD3(+) T cells infiltrated in GVHD target organs were detected in the mice injected with IL-22. Meanwhile, transcription factor T-bet, RORγt and AhR respectively associated with Th1, Th17 and Th22 cells changed in varying degrees in different GVHD target organs. Furthermore, the increased expression of IL-22R and its downstream protein P-STAT3 were detected in GVHD mice with IL-22 treated. These results suggested that the pathological role of IL-22 in GVHD target organs contribute to exogenous injected IL-22 as well as secreted IL-22 from the infiltrated allo-reactive effector T cells. In addition, the IL-22R-STAT3 pathway may play important role in GVHD tissue injury and target this way may yield new approaches for reduction of GVHD.
authors.affiliation: Key laboratory of Bone Marrow Stem Cell, Blood Diseases Institute, Xuzhou Medical University, Jiangsu, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Jiangsu, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Key laboratory of Bone Marrow Stem Cell, Blood Diseases Institute, Xuzhou Medical University, Jiangsu, China.
authors.forename: Suhong
authors.initials: S
authors.lastname: Ruan
authors.affiliation: Key laboratory of Bone Marrow Stem Cell, Blood Diseases Institute, Xuzhou Medical University, Jiangsu, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Key laboratory of Bone Marrow Stem Cell, Blood Diseases Institute, Xuzhou Medical University, Jiangsu, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Key laboratory of Bone Marrow Stem Cell, Blood Diseases Institute, Xuzhou Medical University, Jiangsu, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Jiangsu, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Chen
authors.affiliation: Key laboratory of Bone Marrow Stem Cell, Blood Diseases Institute, Xuzhou Medical University, Jiangsu, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Jiangsu, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Pan
authors.affiliation: Key laboratory of Bone Marrow Stem Cell, Blood Diseases Institute, Xuzhou Medical University, Jiangsu, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Jiangsu, China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Key laboratory of Bone Marrow Stem Cell, Blood Diseases Institute, Xuzhou Medical University, Jiangsu, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Yin
authors.affiliation: Key laboratory of Bone Marrow Stem Cell, Blood Diseases Institute, Xuzhou Medical University, Jiangsu, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Jiangsu, China.
authors.forename: Shengyun
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Key laboratory of Bone Marrow Stem Cell, Blood Diseases Institute, Xuzhou Medical University, Jiangsu, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Jiangsu, China. Electronic address: lihmd@163.com.
authors.forename: Kailin
authors.initials: K
authors.lastname: Xu
mesh_terms: D000208:Acute Disease; D000818:Animals; D016026:Bone Marrow Transplantation; D017252:CD3 Complex / Q000378:metabolism; D002465:Cell Movement; D002478:Cells, Cultured; D006086:Graft vs Host Disease / Q000276:immunology*; D007378:Interleukins / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010766:Phosphorylation; D018123:Receptors, Interleukin / Q000378:metabolism*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous; D000097043:Interleukin-22
chemical_list: D017252:CD3 Complex; D007378:Interleukins; D018123:Receptors, Interleukin; D050796:STAT3 Transcription Factor; D000097043:Interleukin-22; C494087:Stat3 protein, mouse; C415369:interleukin-22 receptor
keywords: Allo-BMT; IL-22; T cell; aGVHD