title: Diverse HLA-I Peptide Repertoires of the APC Lines MUTZ3-Derived Immature and Mature Dendritic Cells and THP1-Derived Macrophages.
abstract: Dendritic cells (DCs) and macrophages are specialized APCs that process and present self-Ags for induction of tolerance and foreign Ags to initiate T cell-mediated immunity. Related to differentiation states they have specific phenotypes and functions. However, the impact of these differentiations on Ag processing and presentation remains poorly defined. To gain insight into this, we analyzed and compared the HLA-I peptidomes of MUTZ3-derived human immature and mature DC lines and THP1-derived macrophages by liquid chromatography tandem mass spectrometry. We found that the HLA-I peptidomes were heterogeneous and individualized and were dominated by nonapeptides with similar HLA-I binding affinities and anchor residues. MUTZ3-derived DCs and THP1-derived macrophages were able to sample peptides from source proteins of almost all subcellular locations and were involved in various cellular functions in similar proportion, with preference to proteins involved in cell communication, signal transduction, protein metabolism, and transcription factor/regulator activity.
authors.affiliation: Klinische Forschergruppe Tumorimmunologie, Klinik für Dermatologie, Venerologie and Allergologie, Charité-Universitätsmedizin Berlin, 10098 Berlin, Germany; and Humboldt Universität zu Berlin, Institut für Biologie, Lebenswissenschaftliche Fakultät, 10115 Berlin, Germany.
authors.forename: Lydon Wainaina
authors.identifier: 0000-0003-2197-6048
authors.initials: LW
authors.lastname: Nyambura
authors.affiliation: Klinische Forschergruppe Tumorimmunologie, Klinik für Dermatologie, Venerologie and Allergologie, Charité-Universitätsmedizin Berlin, 10098 Berlin, Germany; and.
authors.forename: Saulius
authors.initials: S
authors.lastname: Jarmalavicius
authors.affiliation: Klinische Forschergruppe Tumorimmunologie, Klinik für Dermatologie, Venerologie and Allergologie, Charité-Universitätsmedizin Berlin, 10098 Berlin, Germany; and.
authors.forename: Renato Brito
authors.identifier: 0000-0001-7568-7570
authors.initials: RB
authors.lastname: Baleeiro
authors.affiliation: Klinische Forschergruppe Tumorimmunologie, Klinik für Dermatologie, Venerologie and Allergologie, Charité-Universitätsmedizin Berlin, 10098 Berlin, Germany; and peter.walden@charite.de.
authors.forename: Peter
authors.identifier: 0000-0002-8986-3667
authors.initials: P
authors.lastname: Walden
mesh_terms: D000938:Antigen-Presenting Cells / Q000378:metabolism; D001665:Binding Sites; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*
chemical_list: D015395:Histocompatibility Antigens Class I; D010455:Peptides