title: Characterisation of Conformational and Ligand Binding Properties of Membrane Proteins Using Synchrotron Radiation Circular Dichroism (SRCD).
abstract: Membrane proteins are notoriously difficult to crystallise for use in X-ray crystallographic structural determination, or too complex for NMR structural studies. Circular dichroism (CD) is a fast and relatively easy spectroscopic technique to study protein conformational behaviour in solution. The advantage of synchrotron radiation circular dichroism (SRCD) measured with synchrotron beamlines compared to the CD from benchtop instruments is the extended spectral far-UV region that increases the accuracy of secondary structure estimations, in particular under high ionic strength conditions. Membrane proteins are often available in small quantities, and for this SRCD measured at the Diamond B23 beamline has successfully facilitated molecular recognition studies. This was done by probing the local tertiary structure of aromatic amino acid residues upon addition of chiral or non-chiral ligands using long pathlength cells (1-5 cm) of small volume capacity (70 μl-350 μl). In this chapter we describe the use of SRCD to qualitatively and quantitatively screen ligand binding interactions (exemplified by Sbma, Ace1 and FsrC proteins); to distinguish between functionally similar drugs that exhibit different mechanisms of action towards membrane proteins (exemplified by FsrC); and to identify suitable detergent conditions to observe membrane protein-ligand interactions using stabilised proteins (exemplified by inositol transporters) as well as the stability of membrane proteins (exemplified by GalP, Ace1). The importance of the in solution characterisation of the conformational behaviour and ligand binding properties of proteins in both far- andnear-UV regions and the use of high-throughput CD (HT-CD) using 96- and 384-well multiplates to study the folding effects in various protein crystallisation buffers are also discussed.
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, OX11 0DE, Didcot, UK. rohanah.hussain@diamond.ac.uk.
authors.forename: Rohanah
authors.initials: R
authors.lastname: Hussain
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, OX11 0DE, Didcot, UK.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Siligardi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism / Q000295:instrumentation / Q000379:methods*; D003902:Detergents / Q000494:pharmacology; D057166:High-Throughput Screening Assays; D006801:Humans; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D009994:Osmolar Concentration; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012996:Solutions; D017356:Synchrotrons; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D003902:Detergents; D008024:Ligands; D008565:Membrane Proteins; D012996:Solutions
keywords: Circular dichroism (CD); High throughput-CD (HTCD); Ligand binding; Local tertiary structure; Protein secondary structure; SRCD UV-denaturation assay; Synchrotron radiation circular dichroism (SRCD)