title: Tenuigenin exhibits protective effects against LPS-induced acute kidney injury via inhibiting TLR4/NF-κB signaling pathway.
abstract: Tenuigenin (TNG) has been reported to have various pharmacological activities, such as anti-oxidative and anti-inflammatory activities. However, the protective effects of TNG on lipopolysaccharides (LPS)-induced acute kidney injury (AKI) are still not clear. The aim of this study was to investigate the protective effects and mechanism of TGN on LPS-induced AKI in mice. The kidney histological change, levels of blood urea nitrogen (BUN), and creatinine were measured to assess the protective effects of TNG on LPS-induced AKI. The levels of TNF-α, IL-1β, and IL-6 in serum and kidney tissues were detected by ELISA. The extent of nuclear factor kappa-B (NF-κB) p65 and the expression of Toll-like receptor-4 (TLR4) were detected by western blot analysis. The results showed that TNG markedly attenuated the histological alterations, BUN and creatinine levels in kidney. TNG also suppressed LPS-induced TNF-α, IL-1β, and IL-6 production. Furthermore, the expression of TLR4 and NF-κB activation induced by LPS were markedly inhibited by TNG. In conclusion, this study demonstrated that TNG protected against LPS-induced AKI by inhibiting TLR4/NF-κB signaling pathway.
authors.affiliation: Intensive Care Unit in the first affiliated hospital of Jinzhou Medical University, Jinzhou, 121000 Liaoning, China; Department of Dermatology, Beijing Children's Hospital, Capital Medical University, Beijing, 100045, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Fu
authors.affiliation: Intensive Care Unit in the first affiliated hospital of Jinzhou Medical University, Jinzhou, 121000 Liaoning, China.
authors.forename: Zhansheng
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Intensive Care Unit in the first affiliated hospital of Jinzhou Medical University, Jinzhou, 121000 Liaoning, China.
authors.forename: Xingwei
authors.initials: X
authors.lastname: Di
authors.affiliation: Medical Examination Center in The Second Hospital of Chaoyang, Chaoyan, 122000 Liaoning, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Emergency Department of The Military General Hospital of Beijing, P.L.A. Beijing 100700, China.
authors.forename: Rongbin
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Department of Dermatology in The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, China. Electronic address: duhongyujj@sina.com.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Du
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology* / Q000503:physiopathology; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D019610:Cytoprotection / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015398:Signal Transduction / Q000187:drug effects*; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000378:metabolism*
chemical_list: D016207:Cytokines; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D051197:Toll-Like Receptor 4; D051996:Transcription Factor RelA; C493220:tenuigenin
keywords: Acute kidney injury; LPS; NF-κB; Tenuigenin