title: Adult Versus Pluripotent Stem Cell-Derived Mesenchymal Stem Cells: The Need for More Precise Nomenclature.
abstract: The complexity of human pluripotent stem cell (hPSC) fate represents both opportunity and challenge. In theory, all somatic cell types can be differentiated from hPSCs, opening the door to many opportunities in transplant medicine. However, such clinical applications require high standards of purity and identity, that challenge many existing protocols. This underscores the need for increasing precision in the description of cell identity during hPSC differentiation. We highlight one salient example, namely, the numerous published reports of hPSC-derived mesenchymal stem cells (MSCs). We suggest that many of these reports likely represent an improper use of certain cluster of differentiation (CD) antigens in defining bone marrow-derived MSCs. Instead, most such hPSC-derived mesenchymal cells are likely a complex mixture of embryonic anlagen, primarily of diverse mesodermal and neural crest origins, making precise identification, reproducible manufacture, and uniform differentiation difficult to achieve. We describe a potential path forward that may provide more precision in nomenclature, and cells with higher purity and identity for potential therapeutic use.
authors.affiliation: BioTime, Inc., 1010 Atlantic Ave., Alameda, CA 94501 USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: West
authors.affiliation: BioTime, Inc., 1010 Atlantic Ave., Alameda, CA 94501 USA.
authors.forename: Igor
authors.initials: I
authors.lastname: Nasonkin
authors.affiliation: ReCyte Therapeutics, 1010 Atlantic Ave, Alameda, CA 94501 USA.
authors.forename: David
authors.initials: D
authors.lastname: Larocca
authors.affiliation: OncoCyte Corporation, 1010 Atlantic Ave, Alameda, CA 94501 USA.
authors.forename: Karen B
authors.initials: KB
authors.lastname: Chapman
authors.affiliation: BioTime, Inc., 1010 Atlantic Ave., Alameda, CA 94501 USA ; OrthoCyte Corporation, 1010 Atlantic Ave, Alameda, CA 94501 USA.
authors.forename: Francois
authors.initials: F
authors.lastname: Binette
authors.affiliation: BioTime, Inc., 1010 Atlantic Ave., Alameda, CA 94501 USA.
authors.forename: Hal
authors.initials: H
authors.lastname: Sternberg
keywords: Bone; Cartilage; Clonal embryonic progenitor cells; Embryonic stem cells; Mesenchymal stem cells; Neural crest