title: Quantification of simvastatin in mice plasma by near-infrared and chemometric analysis of spectral data.
abstract: Time and cost saving is an essential requirement in pharmacokinetics and bioequivalence studies. The aim of this study is to use a simple, fast, and nondestructive near-infrared transmission spectroscopic method to quantify simvastatin (SMV) concentrations in mice plasma and also to improve SMV bioavailability by using alpha-lipoic acid as a carrier. Calibration curve was built at a concentration range of 10-250 ng/mL, and HPLC method was considered as a reference method. A partial least squares regression analysis model was used for method development, which gave less root mean square error cross-validation. Comparison of SMV concentrations obtained from both instruments showed no statistically significant differences between all the data. Near-infrared spectroscopy was utilized as a rapid, simple accurate method to quantify drug-plasma concentrations without need for any extraction protocols, and the significant effect of alpha-lipoic acid as a novel carrier to enhance SMV bioavailability is also addressed.
authors.affiliation: Department of Pharmaceutics & Industrial Pharmacy, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Usama A
authors.initials: UA
authors.lastname: Fahmy
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D008297:Male; D051379:Mice; D019821:Simvastatin / Q000097:blood*; D019265:Spectroscopy, Near-Infrared
chemical_list: D019821:Simvastatin
keywords: FTIR; alpha lipoic acid; bioavailability; non invasive; pharmacokinetics