title: Integrated application of transcriptomics and metabolomics yields insights into population-asynchronous ovary development in Coilia nasus.
abstract: Populations of Coilia nasus demonstrate asynchronous ovarian development, which severely restricts artificial breeding and large-scale cultivation. In this study, we used a combination of transcriptomic and metabolomic methods to identify the key signaling pathways and genes regulation affecting ovarian development. We identified 565 compounds and generated 47,049 unigenes from ovary tissue. Fifteen metabolites and 830 genes were significantly up-regulated, while 27 metabolites and 642 genes were significantly down-regulated from stage III to stage IV of ovary development. Meanwhile, 31 metabolites and 1,932 genes were significantly up-regulated, and four metabolites and 764 genes were down-regulated from stage IV to stage V. These differentially expressed genes and metabolites were enriched by MetScape. Forty-three and 50 signaling pathways had important functions from stage III-IV and from stage IV-V in the ovary, respectively. Among the above signaling pathways, 39 played important roles from ovarian stage III-V, including "squalene and cholesterol biosynthesis", "steroid hormone biosynthesis", and "arachidonate metabolism and prostaglandin formation" pathways which may thus have key roles in regulating asynchronous development. These results shed new light on our understanding of the mechanisms responsible for population-asynchronous development in fish.
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Jiangsu, 214081, China.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, 214081, China.
authors.forename: Gangchun
authors.initials: G
authors.lastname: Xu
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, 214081, China.
authors.forename: Fukuan
authors.initials: F
authors.lastname: Du
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, 214081, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, 214081, China.
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Nie
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Jiangsu, 214081, China.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, 214081, China.
authors.forename: Pao
authors.initials: P
authors.lastname: Xu
mesh_terms: D000818:Animals; D053898:Biosynthetic Pathways; D005260:Female; D029941:Fish Proteins / Q000235:genetics*; D005399:Fishes / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D055432:Metabolomics / Q000379:methods*; D010053:Ovary / Q000502:physiology; D017423:Sequence Analysis, RNA / Q000379:methods
chemical_list: D029941:Fish Proteins