title: [Analysis of Heteroplasmy in the Major Noncoding Region of Mitochondrial DNA in the Blood and Atherosclerotic Plaques of Carotid Arteries].
abstract: For identification of somatic mitochondrial DNA (mtDNA) mutations, the mtDNA major noncoding region (D-loop) sequence in blood samples and carotid atherosclerosis plaques from patients with atherosclerosis was analyzed. Five point heteroplasmic positions were observed in 4 of 23 individuals (17%). Only in two cases could heteroplasmy have resulted from somatic mutation, whereas three heteroplasmic positions were found in both vascular tissue and blood. In addition, length heteroplasmy in a polycytosine stretches was registered at nucleotide positions 303-315 in 16 individuals, and also in the 16184-16193 region--in four patients. The results suggest that somatic mtDNA mutations can occur during atherosclerosis, but some heteroplasmic mutations may appear in all tissues, possibly being inherited.
authors.forename: M V
authors.initials: MV
authors.lastname: Golubenko
authors.forename: M S
authors.initials: MS
authors.lastname: Nazarenko
authors.forename: A V
authors.initials: AV
authors.lastname: Frolov
authors.forename: A A
authors.initials: AA
authors.lastname: Sleptsov
authors.forename: A V
authors.initials: AV
authors.lastname: Markov
authors.forename: M E
authors.initials: ME
authors.lastname: Glushkova
authors.forename: O L
authors.initials: OL
authors.lastname: Barbarash
authors.forename: V P
authors.initials: VP
authors.lastname: Puzyrev
mesh_terms: D000368:Aged; D050197:Atherosclerosis / Q000235:genetics*; D002339:Carotid Arteries / Q000473:pathology*; D004272:DNA, Mitochondrial / Q000097:blood / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000235:genetics; D058226:Plaque, Atherosclerotic / Q000097:blood / Q000235:genetics* / Q000473:pathology; D017354:Point Mutation / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
chemical_list: D004272:DNA, Mitochondrial