title: The effect of steroid hormones on the mRNA expression of oct4 and sox2 in uterine tissue of the ovariectomized mice model of menopause.
abstract: BACKGROUND
  The uterus is a dynamic tissue responding to hormonal changes during reproductive cycles. As such, uterine stem cells have been studied in recent years. Transcription factors oct4 and sox2 are critical for effective maintenance of pluripotent cell identity.
  
  
  OBJECTIVE
  The present research evaluated the mRNA expression of oct4 and sox2 in the uterine tissues of ovariectomized mice treated with steroid hormones.
  
  
  METHODS
  In this experimental study, adult virgin female mice were ovariectomized and treated with estradiol 17β (E2), progesterone (P4), and a combination of E2 and P4 (E2 & P4) for 5 days. Uterine tissues were removed, and immunofluorescent (IF) staining and quantitative real-time PCR of oct4 and sox2 markers were performed.
  
  
  RESULTS
  IF showed oct4 and sox2 expression in the uterine endometrium and myometrium among all groups. The mRNA expression of oct4 (p=0.022) and sox2 (p=0.042) in the E2-treated group significantly were decreased compared to that in the control group. By contrast, the mRNA expression of oct4 and sox2 in the P4 (p=0.641 and 0.489 respectively) and E2 & P4-treated groups (p=0.267 and 0.264 respectively) did not show significant differences compared to the control group.
  
  
  CONCLUSIONS
  The results indicate ovarian steroid hormones change the expression of oct4 and sox2 in the mice uterine tissues, which suggest the involvement of steroid hormonal regulation in uterine stem cells.
authors.affiliation: School of Biology, Damghan University, Damghan, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Davoudi
authors.affiliation: School of Biology, Damghan University, Damghan, Iran.; Institute of Biological Sciences, Damghan University, Damghan, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Zavareh
authors.affiliation: School of Biology, Damghan University, Damghan, Iran.; Institute of Biological Sciences, Damghan University, Damghan, Iran.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Ghorbanian
authors.affiliation: School of Biology, Damghan University, Damghan, Iran.; Institute of Biological Sciences, Damghan University, Damghan, Iran.
authors.forename: Seyed Hassan
authors.initials: SH
authors.lastname: Paylakhi
authors.affiliation: School of Biology, Damghan University, Damghan, Iran.; Institute of Biological Sciences, Damghan University, Damghan, Iran.
authors.forename: Seyed Reza
authors.initials: SR
authors.lastname: Mohebbi
keywords: Estradiol; Mice; Progesterone; Uterine; stem cells