title: A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans.
abstract: The elongation phase of transcription by RNA Polymerase II (Pol II) involves numerous events that are tightly coordinated, including RNA processing, histone modification, and chromatin remodeling. RNA splicing factors are associated with elongating Pol II, and the interdependent coupling of splicing and elongation has been documented in several systems. Here we identify a conserved, multi-domain cyclophilin family member, SIG-7, as an essential factor for both normal transcription elongation and co-transcriptional splicing. In embryos depleted for SIG-7, RNA levels for over a thousand zygotically expressed genes are substantially reduced, Pol II becomes significantly reduced at the 3' end of genes, marks of transcription elongation are reduced, and unspliced mRNAs accumulate. Our findings suggest that SIG-7 plays a central role in both Pol II elongation and co-transcriptional splicing and may provide an important link for their coordination and regulation.
authors.affiliation: Biology Department, Emory University, Atlanta, Georgia, United States of America.|Program in Genetics and Molecular Biology, Emory University, Atlanta, Georgia, United States of America.
authors.forename: Jeong H
authors.initials: JH
authors.lastname: Ahn
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, California.
authors.forename: Andreas
authors.initials: A
authors.lastname: Rechsteiner
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, California.
authors.forename: Susan
authors.initials: S
authors.lastname: Strome
authors.affiliation: Biology Department, Emory University, Atlanta, Georgia, United States of America.
authors.forename: William G
authors.initials: WG
authors.lastname: Kelly
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D021983:Cyclophilins / Q000096:biosynthesis / Q000235:genetics*; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D007438:Introns / Q000235:genetics; D010766:Phosphorylation; D034622:RNA Interference; D012319:RNA Polymerase II / Q000235:genetics*; D012326:RNA Splicing / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D029742:Caenorhabditis elegans Proteins; C000619237:SIG-7 protein, C elegans; D012319:RNA Polymerase II; D021983:Cyclophilins