title: A two-stage pretreatment process using dilute hydrochloric acid followed by Fenton oxidation to improve sugar recovery from corn stover.
abstract: A two-stage pretreatment process is proposed in this research in order to improve sugar recovery from corn stover. In the proposed process, corn stover is hydrolyzed by dilute hydrochloric acid to recover xylose, which is followed by a Fenton reagent oxidation to remove lignin. 0.7wt% dilute hydrochloric acid is applied in the first stage pretreatment at 120°C for 40min, resulting in 81.0% xylose removal. Fenton reagent oxidation (1g/L FeSO4·7H2O and 30g/L H2O2) is performed at room temperature (about 20°C) for 12 has a second stage which resulted in 32.9% lignin removal. The glucose yield in the subsequent enzymatic hydrolysis was 71.3% with a very low cellulase dosage (3FPU/g). This two-stage pretreatment is effective due to the hydrolysis of hemicelluloses in the first stage and the removal of lignin in the second stage, resulting in a very high sugar recovery with a low enzyme loading.
authors.affiliation: Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei 230026, PR China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei 230026, PR China. Electronic address: qiyu@mail.ustc.edu.cn.
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei 230026, PR China.
authors.forename: Qiaozhi
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei 230026, PR China.
authors.forename: Tingwei
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510650, PR China.
authors.forename: Longlong
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Forest Biomaterials, North Carolina State University, Raleigh, NC 27695-8005, USA.
authors.forename: Hasan
authors.initials: H
authors.lastname: Jameel
authors.affiliation: Department of Forest Biomaterials, North Carolina State University, Raleigh, NC 27695-8005, USA.
authors.forename: Hou-Min
authors.initials: HM
authors.lastname: Chang
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000737:chemistry / Q000302:isolation & purification; D006851:Hydrochloric Acid / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006868:Hydrolysis; D007501:Iron / Q000737:chemistry; D008031:Lignin / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D009005:Monosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D018520:Plant Shoots / Q000737:chemistry; D013696:Temperature; D014961:X-Ray Diffraction; D014994:Xylose / Q000737:chemistry / Q000302:isolation & purification; D003313:Zea mays / Q000737:chemistry*
chemical_list: C045076:Fenton's reagent; D009005:Monosaccharides; D008031:Lignin; D014994:Xylose; D006861:Hydrogen Peroxide; D007501:Iron; D002480:Cellulase; D005947:Glucose; D006851:Hydrochloric Acid
keywords: Corn stover; Dilute acid; Enzymatic hydrolysis; Fenton oxidation; Pretreatment