title: Subchronic administration of phencyclidine produces hypermethylation in the parvalbumin gene promoter in rat brain.
abstract: A deficit in parvalbumin neurons is found in schizophrenia and several animal models of the disease. In this preliminary study, we determined whether one such model, phencyclidine (PCP) administration, results in changes in DNA methylation in the rat Pvalb promoter.
  
  
  
  DNA from hippocampus and prefrontal cortex from rats, which 6 weeks previously received either 2 mg/kg PCP or vehicle for 7 days, underwent bisulphite pyrosequencing to determine methylation.
  
  
  
  PCP administration induced significantly greater methylation at one of two Pvalb CpG sites in both prefrontal cortex and hippocampus, while no significant difference was found in long interspersed nucleotide element-1, a global measure of DNA methylation.
  
  
  
  Subchronic PCP administration results in a specific hypermethylation in the Pvalb promoter which may contribute to parvalbumin deficits in this animal model of psychosis.
authors.affiliation: Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, S1 1WB, UK.
authors.forename: Helene A
authors.initials: HA
authors.lastname: Fachim
authors.affiliation: Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, S1 1WB, UK.
authors.forename: Umarat
authors.initials: U
authors.lastname: Srisawat
authors.affiliation: Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, S1 1WB, UK.
authors.forename: Caroline F
authors.initials: CF
authors.lastname: Dalton
authors.affiliation: Manchester Pharmacy School, University of Manchester, Manchester, M13 9PT, UK.
authors.forename: Michael K
authors.initials: MK
authors.lastname: Harte
authors.affiliation: Manchester Pharmacy School, University of Manchester, Manchester, M13 9PT, UK.
authors.forename: Samuel
authors.initials: S
authors.lastname: Marsh
authors.affiliation: Manchester Pharmacy School, University of Manchester, Manchester, M13 9PT, UK.
authors.forename: Joanna C
authors.initials: JC
authors.lastname: Neill
authors.affiliation: Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, S1 1WB, UK.
authors.forename: Gavin P
authors.initials: GP
authors.lastname: Reynolds
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D018899:CpG Islands; D019175:DNA Methylation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D005260:Female; D020084:Long Interspersed Nucleotide Elements; D010320:Parvalbumins / Q000235:genetics*; D010622:Phencyclidine / Q000008:administration & dosage / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D051381:Rats
chemical_list: D018691:Excitatory Amino Acid Antagonists; D010320:Parvalbumins; D010622:Phencyclidine
keywords: DNA methylation; novel object recognition; parvalbumin; phencyclidine; rat; schizophrenia