title: Evaluation of the Efficiency of Two Different Freezing Media and Two Different Protocols to Preserve Human Spermatozoa from Cryoinjury.
abstract: It is universally recognized that cryopreservation impairs sperm quality. In order to improve postthawing sperm survival and motility, media of different composition and different protocols have been proposed. However, no clear evidence is available to understand which are the most efficient protocol and medium for sperm cryopreservation. The present study evaluates the efficiency of two different cryopreservation protocols and two common freezing media (FM) containing different cryoprotectants (CPs), TEST Yolk Buffer (TYB) and Sperm Freeze (SF), to preserve human sperm quality. Our data suggest that TYB is better than SF both in terms of postthaw viability and in terms of progressive motility, while the direct addition of FM to the sperm sample resulted in the most efficient protocol in terms of postthaw viability but not in terms of progressive motility.
authors.affiliation: FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy.
authors.forename: Gemma
authors.identifier: 0000-0002-6224-4997
authors.initials: G
authors.lastname: Fabozzi
authors.affiliation: FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy.
authors.forename: Maria Flavia
authors.initials: MF
authors.lastname: Starita
authors.affiliation: FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy.
authors.forename: Emilia
authors.initials: E
authors.lastname: Rega
authors.affiliation: FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy.
authors.forename: Alessandra
authors.identifier: 0000-0003-3092-3546
authors.initials: A
authors.lastname: Alteri
authors.affiliation: FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy.
authors.forename: Antonio
authors.identifier: 0000-0001-9412-5434
authors.initials: A
authors.lastname: Colicchia
authors.affiliation: FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Piscitelli
authors.affiliation: FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy.
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Giannini