title: Quality assessment of ice-stored tropical yellowfin tuna (Thunnus albacares) and influence of vacuum and modified atmosphere packaging.
abstract: Metagenomic, microbial, chemical and sensory analyses of Thunnus albacares from Martinique stored in ice (AIR - 0 °C), vacuum (VP - 4/8 °C) and modified atmosphere packaging (MAP - 4/8 °C) (70% CO2 - 30% O2) were carried out. The organoleptic rejection of AIR tuna was observed at day 13 when total bacterial counts equaled 10(6)-10(7) CFU g(-1). No extension of shelf-life was provided by VP and MAP. According to 16S rRNA gene sequence analyzed by Illumina MiSeq and PCR-TTGE, Rhodanobacter terrae was the main species of the freshly caught tuna. At the sensory rejection time, Brochothrix thermosphacta and Pseudomonas dominated the AIR products while B. thermosphacta alone or a mix of B. thermosphacta, Enterobacteriaceae and lactic acid bacteria (LAB) dominated the microbiota of MAP and VP products, respectively. The pH value remained stable in all trials, ranging from 5.77 to 5.97. Total volatile basic nitrogen (TVBN) and trimethylamine (TMA-N) concentrations were weak and not significantly different between batches. Lipid oxidation increased in the samples containing O2 (MAP > AIR). The initial concentration of histamine was high (75-78 mg kg(-1)) and stable up to 8 days but then significantly decreased in all trials to reach 25-30 mg kg(-1), probably due to the presence of histamine-decomposing bacteria.
authors.affiliation: Pôle Agroalimentaire Régional de Martinique (PARM), Impasse Petit-Morne, N° 375, 97232 Lamentin, Martinique; Université des Antilles, Département Scientifique Inter facultaire (DSI), EA929 AIHP-GEODE (groupe BIOSPHERES), BP 7209, 97275 Schœlcher, Martinique; Ifremer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies (EM(3)B), rue de l'Ile d'Yeu, BP 21105, 44300 Nantes, France. Electronic address: adele.silbande@gmail.com.
authors.forename: Adèle
authors.initials: A
authors.lastname: Silbande
authors.affiliation: Pôle Agroalimentaire Régional de Martinique (PARM), Impasse Petit-Morne, N° 375, 97232 Lamentin, Martinique. Electronic address: adenet@parm.asso.fr.
authors.forename: Sandra
authors.initials: S
authors.lastname: Adenet
authors.affiliation: Université des Antilles, Département Scientifique Inter facultaire (DSI), EA929 AIHP-GEODE (groupe BIOSPHERES), BP 7209, 97275 Schœlcher, Martinique. Electronic address: jsmithra@martinique.univ-ag.fr.
authors.forename: Juliette
authors.initials: J
authors.lastname: Smith-Ravin
authors.affiliation: Ifremer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies (EM(3)B), rue de l'Ile d'Yeu, BP 21105, 44300 Nantes, France. Electronic address: Jean.Jacques.Joffraud@ifremer.fr.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Joffraud
authors.affiliation: Pôle Agroalimentaire Régional de Martinique (PARM), Impasse Petit-Morne, N° 375, 97232 Lamentin, Martinique. Electronic address: rochefort@parm.asso.fr.
authors.forename: Katia
authors.initials: K
authors.lastname: Rochefort
authors.affiliation: Ifremer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies (EM(3)B), rue de l'Ile d'Yeu, BP 21105, 44300 Nantes, France. Electronic address: Francoise.Leroi@ifremer.fr.
authors.forename: Françoise
authors.initials: F
authors.lastname: Leroi
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D058640:Brochothrix / Q000235:genetics / Q000302:isolation & purification; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D005516:Food Microbiology; D018857:Food Packaging / Q000592:standards*; D061353:Food Storage / Q000379:methods / Q000592:standards*; D020459:Genes, rRNA; D006632:Histamine / Q000032:analysis; D007053:Ice; D056186:Metagenomics; D064307:Microbiota / Q000235:genetics / Q000502:physiology; D009584:Nitrogen / Q000032:analysis; D011786:Quality Control; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017747:Seafood / Q000032:analysis / Q000382:microbiology*; D013649:Taste; D014413:Tuna / Q000382:microbiology*; D014618:Vacuum
chemical_list: D007053:Ice; D012336:RNA, Ribosomal, 16S; D006632:Histamine; D009584:Nitrogen
keywords: 16S rRNA gene; Fish; Microbiology; NGS; Seafood; Sensory