title: Preparation of Drosophila Follicles for Transmission Electron Microscopy.
abstract: Transmission Electron Microscopy (TEM) provides high resolution and accuracy at the subcellular level for observing and investigating cellular structures. This is essential for understanding a large variety of cellular processes. In this chapter, we describe a detailed protocol for preparing Drosophila follicles in order to be used as a specimen for transmission electron microscopy.
authors.affiliation: School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK.
authors.forename: Anthimi
authors.initials: A
authors.lastname: Palara
authors.affiliation: School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK.
authors.forename: Antonia P
authors.initials: AP
authors.lastname: Sagona
authors.affiliation: School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK. I.Nezis@warwick.ac.uk.
authors.forename: Ioannis P
authors.initials: IP
authors.lastname: Nezis
mesh_terms: D000818:Animals; D004330:Drosophila / Q000166:cytology* / Q000648:ultrastructure*; D005260:Female; D042282:Microdissection; D046529:Microscopy, Electron, Transmission / Q000379:methods; D006080:Ovarian Follicle / Q000648:ultrastructure*; D010053:Ovary / Q000166:cytology / Q000648:ultrastructure
keywords: Drosophila egg chamber; Oogenesis; Subcellular morphology; Transmission electron microscopy