title: ST6GALNAC5 Expression Decreases the Interactions between Breast Cancer Cells and the Human Blood-Brain Barrier.
abstract: The ST6GALNAC5 gene that encodes an α2,6-sialyltransferase involved in the biosynthesis of α-series gangliosides, was previously identified as one of the genes that mediate breast cancer metastasis to the brain. We have shown that the expression of ST6GALNAC5 in MDA-MB-231 breast cancer cells resulted in the expression of GD1α ganglioside at the cell surface. By using a human blood-brain barrier in vitro model recently developed, consisting in CD34⁺ derived endothelial cells co-cultivated with pericytes, we show that ST6GALNAC5 expression decreased the interactions between the breast cancer cells and the human blood-brain barrier.
authors.affiliation: Université d'Artois (UArtois), EA2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Lens F-62300, France. aurore.drolez@univ-artois.fr.
authors.forename: Aurore
authors.initials: A
authors.lastname: Drolez
authors.affiliation: Université d'Artois (UArtois), EA2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Lens F-62300, France. elodie.vandenhaute@univ-artois.fr.
authors.forename: Elodie
authors.initials: E
authors.lastname: Vandenhaute
authors.affiliation: Structural and Functional Glycobiology Unit, Unité Mixte de Recherche (UMR) du Centre National de la Recherche Scientifique (CNRS) 8576, University of Lille, Villeneuve d'Ascq F-59655, France. cl-ment.delannoy@etudiant.univ-lille1.fr.
authors.forename: Clément Philippe
authors.initials: CP
authors.lastname: Delannoy
authors.affiliation: Structural and Functional Glycobiology Unit, Unité Mixte de Recherche (UMR) du Centre National de la Recherche Scientifique (CNRS) 8576, University of Lille, Villeneuve d'Ascq F-59655, France. justine.dewald@etudiant.univ-lille1.fr.
authors.forename: Justine Hélène
authors.initials: JH
authors.lastname: Dewald
authors.affiliation: Université d'Artois (UArtois), EA2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Lens F-62300, France. fabien.gosselet@univ-artois.fr.
authors.forename: Fabien
authors.initials: F
authors.lastname: Gosselet
authors.affiliation: Université d'Artois (UArtois), EA2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Lens F-62300, France. romeo.cecchelli@univ-artois.fr.
authors.forename: Romeo
authors.initials: R
authors.lastname: Cecchelli
authors.affiliation: Cell Plasticity and Cancer, U908 INSERM, University of Lille, Villeneuve d'Ascq F-59655, France. sylvain.julien@univ-lille1.fr.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Julien
authors.affiliation: Université d'Artois (UArtois), EA2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Lens F-62300, France. mpierre.dehouck@univ-artois.fr.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Dehouck
authors.affiliation: Structural and Functional Glycobiology Unit, Unité Mixte de Recherche (UMR) du Centre National de la Recherche Scientifique (CNRS) 8576, University of Lille, Villeneuve d'Ascq F-59655, France. philippe.delannoy@univ-lille1.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Delannoy
authors.affiliation: Université d'Artois (UArtois), EA2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Lens F-62300, France. caroline.mysiorek@univ-artois.fr.
authors.forename: Caroline
authors.initials: C
authors.lastname: Mysiorek
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001812:Blood-Brain Barrier / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D051379:Mice; D020286:Pericytes / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism*
chemical_list: D018952:Antigens, CD34; C064020:ganglioside GD1alpha; D005677:G(M1) Ganglioside; D012799:Sialyltransferases
keywords: GD1α; ST6GALNAC5; blood-brain barrier; brain metastasis; breast cancer; gangliosides; sialyltransferase