title: Identification of candidate genes related to calanolide biosynthesis by transcriptome sequencing of Calophyllum brasiliense (Calophyllaceae).
abstract: Calophyllum brasiliense is highlighted as an important resource of calanolides, which are dipyranocoumarins that inhibit the reverse transcriptase of human immunodeficiency virus type 1 (HIV-1 RT). Despite having great medicinal importance, enzymes involved in calanolide, biosynthesis and the pathway itself, are still largely unknown. Additionally, no genomic resources exist for this plant species.
  
  
  
  In this work, we first analyzed the transcriptome of C. brasiliense leaves, stem, and roots using a RNA-seq strategy, which provided a dataset for functional gene mining. According to the structures of the calanolides, putative biosynthetic pathways were proposed. Finally, candidate unigenes in the transcriptome dataset, potentially involved in umbelliferone and calanolide (angular pyranocoumarin) biosynthetic pathways, were screened using mainly homology-based BLAST and phylogenetic analyses.
  
  
  
  The unigene dataset that was generated in this study provides an important resource for further molecular studies of C. brasiliense, especially for functional analysis of candidate genes involved in the biosynthetic pathways of linear and angular pyranocoumarins.
authors.affiliation: Facultad de Ciencias de la Salud, Universidad Anáhuac, 52786, Estado de México, México.|Laboratorio de Bioquímica y Biología Molecular, Escuela Médico Militar, Universidad del Ejército y Fuerza Aérea, 11200, Ciudad de México, México.
authors.forename: Hilda-Beatriz
authors.initials: HB
authors.lastname: Gómez-Robledo
authors.affiliation: Departamento de Biotecnología, Universidad Autónoma Metropolitana Unidad Iztapalapa (UAM-Iztapalapa), 09340, Ciudad de México, México.
authors.forename: Francisco
authors.initials: F
authors.lastname: Cruz-Sosa
authors.affiliation: Departamento de Madera, Celulosa y Papel, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, 45100, Zapopan, Jalisco, México.
authors.forename: Antonio
authors.initials: A
authors.lastname: Bernabé-Antonio
authors.affiliation: Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C, 91070, Xalapa, Veracruz, México.
authors.forename: Antonio
authors.initials: A
authors.lastname: Guerrero-Analco
authors.affiliation: Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C, 91070, Xalapa, Veracruz, México.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Olivares-Romero
authors.affiliation: Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C, 91070, Xalapa, Veracruz, México.
authors.forename: Alexandro
authors.initials: A
authors.lastname: Alonso-Sánchez
authors.affiliation: Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C, 91070, Xalapa, Veracruz, México.
authors.forename: Emanuel
authors.initials: E
authors.lastname: Villafán
authors.affiliation: Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C, 91070, Xalapa, Veracruz, México. enrique.ibarra@inecol.mx.
authors.forename: Enrique
authors.identifier: 0000-0003-1441-1938
authors.initials: E
authors.lastname: Ibarra-Laclette
mesh_terms: D029759:Calophyllum / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D039681:Pyranocoumarins / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D039681:Pyranocoumarins