title: [Calcitonin gene-related peptide-induced osteogenic differentiation of mouse bone marrow stromal cells through Hippo pathway in vitro].
abstract: OBJECTIVE
  Previous studies have clarified that calcitonin gene-related peptide (CGRP) can promote the biologi- cal activity of osteoblasts. To further reveal the role of CGRP in bone repair, we studied its influence on osteogenic differentia- tion of mouse bone marrow stromal cells (BMSCs) and initially explored the effect of the Hippo signaling pathway with this process.
  
  
  METHODS
  BMSCs were induced to osteogenic differentiate osteoblasts by different concentrations of CGRP for a screening of the optimal concentration. CGRP was added in BMSCs, then the activity of alkaline phosphatase (ALP) and the number of mineralized nodules were examined by specific ALP kits after 48 hours and alizarin red staining fluid after 7 days, respectively. The protein expression of p-Mst1/2 was measured by Western blot. Verteporfin was used to block the downstream Yap signaling. The mRNA expression of collagen type I (Col I) and runt-related transcription factor 2 (Runx2) were detected by reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  Compared to the blank group, different concentrations of CGRP (10⁻⁹, 10⁻⁸, 10⁻⁷ mol · L⁻¹), especially 10⁻⁸ mol · L⁻¹, significantly increased the ALP activity of BMSCs (P < 0.05). Alizarin red staining also showed more mineralized nodules in 10⁻⁸ mol · L⁻¹ group. The expression of p-Mst1/2 increased in the CGRP group (P < 0.05). Verteporfin treatment effectively decreased the mRNA expression of Runx2 and Col I (P < 0.05).
  
  
  CONCLUSIONS
  The Hippo signaling pathway plays a role in CGRP-induced osteogenic differentiation in mouse BMSCs.
authors.forename: Wang
authors.initials: W
authors.lastname: Fei
authors.forename: Zhang
authors.initials: Z
authors.lastname: Huiyu
authors.forename: Dou
authors.initials: D
authors.lastname: Yuxin
authors.forename: Li
authors.initials: L
authors.lastname: Shiting
authors.forename: Zhang
authors.initials: Z
authors.lastname: Gang
authors.forename: Tan
authors.initials: T
authors.lastname: Yinghui
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D002116:Calcitonin / Q000235:genetics / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D010006:Osteoblasts; D010012:Osteogenesis / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D002116:Calcitonin; D000469:Alkaline Phosphatase; D015740:Calcitonin Gene-Related Peptide