title: Communication-Microelectrode Detection of Cholesterol Efflux from the Human Buccel Mucosa.
abstract: It has previously demonstrated that cholesterol efflux from the cell plasma membrane is increased in a mouse model of cystic fibrosis (CF) compared to a wild-type control. A noninvasive means of characterizing plasma membrane cholesterol efflux at the surface of airway tissue of CF patients is needed to extend the trends found in animal models of CF to the human disease state. Microelectrode-induced cholesterol efflux from the plasma membrane of cells at the surface of tissue is proposed as a strategy to demonstrate increased cholesterol efflux for CF in human subjects. Data demonstrating detection of cholesterol efflux from the human buccal mucosa is reported as proof-of-concept for anin vivodiagnostic assay.
authors.affiliation: Department of Chemistry, Case Western Reserve University and Rainbow Babies and Children's Hospital, Cleveland, Ohio 44106.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Pediatrics and Pharmacology, Case Western Reserve University and Rainbow Babies and Children's Hospital, Cleveland, Ohio 44106.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Kelley
authors.affiliation: Department of Biology, Case Western Reserve University and Rainbow Babies and Children's Hospital, Cleveland, Ohio 44106.
authors.forename: Hillel J
authors.initials: HJ
authors.lastname: Chiel
authors.affiliation: Department of Chemistry, Case Western Reserve University and Rainbow Babies and Children's Hospital, Cleveland, Ohio 44106.
authors.forename: James D
authors.initials: JD
authors.lastname: Burgess