title: [Effects on wound bed of deep burn following eschar excision with different wound management in rabbits].
abstract: OBJECTIVE
  To compare the effects on wound bed of deep burn following eschar excision with different wound management in rabbits.
  
  
  METHODS
  Eighteen full-thickness burns models of Japanese white rabbits were established. They were randomly divided into 3 groups of traditional dressing, biological dressing and negative pressure wound therapy (NPWT) (n=6 each), according to the random number table. Eschar excision was performed three days later. The wound bed was observed and wound tissue was harvested for counting the quantity of bacteria, tissue dry wet ratio, measuring the level of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β and IL-6, the amount of collagen fibers and the microvessel density instantly and again seven days later. Statistical analyses were performed.
  
  
  RESULTS
  The NPWT group was better than other groups by observing the wound bed. The quantity of bacteria of traditional dressing group, biological dressing group and NPWT group at the time point of seven days after escharectomy turned out to be (9.4±1.5)×10(4,) (8.1±2.7)×10(4,) (3.9±0.7)×10(4) cfu/g, the NPWT group was significantly lower than traditional dressing group and biological dressing group (both P<0.05), and all lower than that at the time point of the day when escharectomy was performed (576.9±169.5)×10(4,) (589.9±99.6)×10(4,) (583.0±160.4)×10(4) cfu/g ( all P<0.05). There were no statistically significant differences among three groups at two time points in tissue dry wet ratio (all P>0.05). The IL-6 of biological dressing group was higher than that of traditional dressing group at the time point of seven days after the eschar excision was performed[(94±10) vs (76±8) ng/L, P<0.05]. The amount of collagen fibers of three group at the time point of seven days after escharectomy turned out to be (60±9), (55±12), (77±17). The NPWT group was significantly higher than traditional dressing group and biological dressing group (P<0.05), and all higher than that at the time point of the day when escharectomy was performed[(39±6), (39±11), (38±6)](all P<0.05). The microvessel density of three groups at the time point of seven days after escharectomy turned out to be (42±6), (53±4), (82±10). The NPWT group was higher than that of the other two groups, and biological dressing group was higher than that of traditional dressing group (all P<0.05). The biological dressing group and NPWT group were both higher than that of the day when the eschar excision was performed (36±5) and (36±5) (P<0.05).
  
  
  CONCLUSIONS
  NPWT is the optimal selection for wound to inhibit the growth of bacteria, promote the accumulation of collagen and tissue vascularization. But these managements have similar effects on reducing tissue edema and inflammatory reaction.
authors.affiliation: Burns and Skin Repair Surgery, Third Affiliated Hospital, Whenzhou Medical University, Ruian 325200, China.
authors.forename: D J
authors.initials: DJ
authors.lastname: Xue
authors.forename: J H
authors.initials: JH
authors.lastname: Lin
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: W X
authors.initials: WX
authors.lastname: Huang
authors.forename: G L
authors.initials: GL
authors.lastname: Su
mesh_terms: D000818:Animals; D002056:Burns; D007249:Inflammation; D054843:Negative-Pressure Wound Therapy; D011817:Rabbits; D014945:Wound Healing