title: 7-Hydroxytropolone produced and utilized as an iron-scavenger by Pseudomonas donghuensis.
abstract: Pseudomonas donghuensis can excrete large quantities of iron chelating substances in iron-restricted environments. At least two kinds of iron-chelator can be found in the culture supernatant: fluorescent siderophores pyoverdins, and an ethyl acetate-extractable non-fluorescent substance. The non-fluorescent substance was the dominant contributor to the iron chelating activity of the culture supernatant of P. donghuensis. Electron ionization mass spectrometry, NMR spectroscopy, and IR spectroscopy identified the non-fluorescent iron-chelator as 7-hydroxytropolone. The stoichiometry of 7-hydroxytropolone ferric complex was determined to be 2:1 by the continuous variation method. The production of 7-hydroxytropolone was repressible by iron in the medium. Moreover, the inhibited growth of doubly siderophore-deficient strain of P. donghuensis under iron-limiting conditions could be partly restored by 7-hydroxytropolone. Thus, 7-hydroxytropolone was considered to play a previously undiscovered role as an iron-scavenger for P. donghuensis.
authors.affiliation: Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), State Key Laboratory of Virology, Wuhan University, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, 430072, Wuhan, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), State Key Laboratory of Virology, Wuhan University, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, 430072, Wuhan, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.affiliation: Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), State Key Laboratory of Virology, Wuhan University, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, 430072, Wuhan, People's Republic of China.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Yu
authors.affiliation: Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), State Key Laboratory of Virology, Wuhan University, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, 430072, Wuhan, People's Republic of China. zxxie@whu.edu.cn.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Xie
mesh_terms: D004305:Dose-Response Relationship, Drug; D007502:Iron Chelating Agents / Q000378:metabolism* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000378:metabolism* / Q000494:pharmacology; D011549:Pseudomonas / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014334:Tropolone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology
chemical_list: D007502:Iron Chelating Agents; D009942:Organometallic Compounds; C035995:7-hydroxytropolone; D014334:Tropolone
keywords: 7-Hydroxytropolone; 7-Hydroxytropolone ferric complex; Pseudomonas donghuensis; Siderophore