title: Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio.
abstract: The worldwide cancer incidences are remarkable despite the advancement in cancer drug discovery field, highlighting the need for new therapies focusing on cancer cell and its microenvironment, including inflammation. Several species of Drosera (family: Droseraceae) are used in various traditional as well as homeopathic systems of medicine. Drosera burmannii Vahl. is also enlisted in French Pharmacopoeia in 1965 for the treatment of inflammatory diseases, including chronic bronchitis, asthma and whooping cough. The present study is designed to substantiate the potential of D. burmannii in in vitro anticancer activity and its relation with anti-inflammatory property. In vitro anticancer study revealed that DBME is inhibiting the proliferation of MCF-7 cells without affecting the viability of other malignant and non-malignant cells. DBME induced G2/M phase arrest and apoptosis in MCF-7 cells by suppressing the expression of cyclin A1, cyclin B1 and Cdk-1 and increasing the expression of p53, Bax/Bcl-2 ratio leading to activation of caspases and PARP degradation. Presence of caspase-8 (Z-IETD-fmk) and caspase-9 (Z-LEHD-fmk) inhibitors alone did prevent the apoptosis partially while apoptosis prevention was significantly observed when used in combination, suggesting vital role of caspases in DBME-induced apoptosis in MCF-7 cells. DBME also downregulated LPS-induced increased expression of iNOS, COX-2 and TNF-α along with suppression on intracellular ROS production that confirms the potential of DBME as anti-inflammatory extract. GCMS analysis revealed the presence of four major compounds hexadecanoic acid, tetradecanoic acid, hexadecen-1-ol, trans-9 and 1-tetradecanol along with some other fatty acid derivatives and carotenoids (Beta-doradecin) in DBME. These findings confirmed the anti-inflammatory activity of DBME, which is already listed in French Pharmacopeia in 1965. Here we have additionally reported the anti-breast cancer activity of DBME and its relation to the anti-inflammatory potential. Hence, an ethnopharmacological approach can be considered as useful tool for the discovery of new drug leads.
authors.affiliation: Division of Molecular Medicine, Bose Institute , P-1/12 C. I. T. Scheme VII M, Kolkata 700054, India.
authors.forename: N B
authors.initials: NB
authors.lastname: Ghate
authors.affiliation: Division of Molecular Medicine, Bose Institute , P-1/12 C. I. T. Scheme VII M, Kolkata 700054, India.
authors.forename: A
authors.initials: A
authors.lastname: Das
authors.affiliation: Division of Molecular Medicine, Bose Institute , P-1/12 C. I. T. Scheme VII M, Kolkata 700054, India.
authors.forename: D
authors.initials: D
authors.lastname: Chaudhuri
authors.affiliation: Division of Molecular Medicine, Bose Institute , P-1/12 C. I. T. Scheme VII M, Kolkata 700054, India.
authors.forename: S
authors.initials: S
authors.lastname: Panja
authors.affiliation: Division of Molecular Medicine, Bose Institute , P-1/12 C. I. T. Scheme VII M, Kolkata 700054, India.
authors.forename: N
authors.initials: N
authors.lastname: Mandal