title: Carboxymethylated degraded polysaccharides from Enteromorpha prolifera: Preparation and in vitro antioxidant activity.
abstract: In order to improve the bioactivities of the polysaccharide from Enteromorpha prolifera (PE), crude PE (Mw 1400kDa) was degraded to low molecular weight polysaccharide (44kDa) in the presence of hydrogen peroxide/ascorbic acid, followed by carboxymethylation. The reaction conditions for carboxymethylation of degraded polysaccharide (DPE) were optimized by Response Surface Methodology. The carboxymethyled degraded polysaccharide (CDPE) obtained under optimized conditions, with a degree of carboxymethylation of 0.849, was characterized by FT-IR and (13)C NMR. The molecular weight of CDPE was measured to be 53.7kDa. CDPE was evaluated for its antioxidant activity by determining the ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and superoxide anion radicals, and by determining the ferric reducing power. The antioxidant activity of CDPE was found to be greatly improved in comparison with degraded polysaccharide (DPE) and crude polysaccharide from Enteromorpha prolifera (PE).
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China.
authors.forename: Mei-Jia
authors.initials: MJ
authors.lastname: Shi
authors.affiliation: College of Tourism & Food, Shanghai Business School, Shanghai 200235, PR China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wei
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China.
authors.forename: Bing-Jie
authors.initials: BJ
authors.lastname: Chen
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China.
authors.forename: De-Yin
authors.initials: DY
authors.lastname: Zhao
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Cui
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha, Hangzhou, Zhejiang 310018, PR China. Electronic address: taozhou@zjgsu.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
mesh_terms: D000975:Antioxidants; D050260:Carbohydrate Metabolism; D066298:In Vitro Techniques; D011134:Polysaccharides / Q000737:chemistry*; D044445:Ulva / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides
keywords: Antioxidant activity; Carboxymethylation; Enteromorpha prolifera; Polysaccharides