title: Potential of a lytic bacteriophage to disrupt Acinetobacter baumannii biofilms in vitro.
abstract: The ability of Acinetobacter baumannii to form biofilms and develop antibiotic resistance makes it difficult to control infections caused by this bacterium. In this study, we explored the potential of a lytic bacteriophage to disrupt A. baumannii biofilms.
  
  
  
  The potential of the lytic bacteriophage to disrupt A. baumannii biofilms was assessed by performing electron microscopy, live/dead bacterial staining, crystal violet staining and by determining adenosine triphosphate release.
  
  
  
  The bacteriophage inhibited the formation of and disrupted preformed A. baumannii biofilms. Results of disinfection assay showed that the lytic bacteriophage lysed A. baumannii cells suspended in blood or grown on metal surfaces.
  
  
  
  These results suggest the potential of the lytic bacteriophage to disrupt A. baumannii biofilms.
authors.affiliation: Department of Graduate, Hebei North University, Zhangjiakou, Hebei 075000, China.|Department of Respiratory & Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Mi
authors.affiliation: Department of Respiratory & Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Niu
authors.affiliation: Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: An
authors.affiliation: Department of Respiratory & Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Respiratory & Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Respiratory & Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Respiratory & Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, China.
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Xianglilan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Respiratory Medicine, the First Hospital of Changsha, Changsha, Hunan 410000, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Peng
authors.affiliation: Department of Respiratory & Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, China.
authors.forename: Puyuan
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Yigang
authors.initials: Y
authors.lastname: Tong
authors.affiliation: Department of Respiratory & Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Bai
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology / Q000628:therapy / Q000821:virology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000821:virology*; D000255:Adenosine Triphosphate / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001435:Bacteriophages / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002681:China; D004203:Disinfection / Q000379:methods; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D000071059:Phage Therapy / Q000379:methods*; D013183:Sputum / Q000382:microbiology; D013194:Staining and Labeling
chemical_list: D000900:Anti-Bacterial Agents; D000255:Adenosine Triphosphate
keywords: Acinetobacter baumannii; bacteriophage; biofilm; disinfection; resistance