title: The Physcomitrella patens System for Transient Gene Expression Assays.
abstract: Transient expression assays are valuable techniques to study in vivo the transcriptional regulation of gene expression. These methods allow to assess the transcriptional properties of a given transcription factor (TF) or a complex of regulatory proteins against specific DNA motifs, called cis-regulatory elements. Here, we describe a fast, efficient, and reliable method based on the use of Physcomitrella patens protoplasts that allows the study of gene expression in a qualitative and quantitative manner by combining the advantage of GFP (green fluorescent protein) as a marker of promoter activity with flow cytometry for accurate measurement of fluorescence in individual cells.
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, UMR1318, RD10, 78026, Versailles, France.|AgroParisTech, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, UMR1318, RD10, 78026, Versailles, France.
authors.forename: Johanne
authors.initials: J
authors.lastname: Thévenin
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, UMR1318, RD10, 78026, Versailles, France.|AgroParisTech, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, UMR1318, RD10, 78026, Versailles, France.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Xu
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, UMR1318, RD10, 78026, Versailles, France.|AgroParisTech, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, UMR1318, RD10, 78026, Versailles, France.
authors.forename: Louise
authors.initials: L
authors.lastname: Vaisman
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, UMR1318, RD10, 78026, Versailles, France.|AgroParisTech, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, UMR1318, RD10, 78026, Versailles, France.
authors.forename: Loïc
authors.initials: L
authors.lastname: Lepiniec
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, UMR1318, RD10, 78026, Versailles, France.|AgroParisTech, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, UMR1318, RD10, 78026, Versailles, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Dubreucq
authors.affiliation: INRA, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, UMR1318, RD10, 78026, Versailles, France. christian.dubos@supagro.inra.fr.|AgroParisTech, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, UMR1318, RD10, 78026, Versailles, France. christian.dubos@supagro.inra.fr.|Biochimie et Physiologie Moleculaire des Plantes, UMR 5004, INRA/CNRS/SupAgro-M/UM2, 34060, Montpellier Cedex 1, France. christian.dubos@supagro.inra.fr.|Unité de Biochimie et Physiologie Moléculaire des Plantes (B&PMP), 2 Place Pierre Viala, 34060, Montpellier Cedex 02, France. christian.dubos@supagro.inra.fr.
authors.forename: Christian
authors.initials: C
authors.lastname: Dubos
mesh_terms: D019068:Bryopsida / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D011523:Protoplasts / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D010940:Plant Proteins; D049452:Green Fluorescent Proteins
keywords: Physcomitrella patens; Protoplasts; Subcellular localization; Transcription factor; Transient expression