title: Lipid composition in a strain of Bacillus subtilis, a producer of iturin A lipopeptides that are active against uropathogenic bacteria.
abstract: Urinary tract infections are a common disease in humans. Therefore, new methods are needed to destroy biofilms that are formed by uropathogens. Iturin A lipopeptides (LPs) C14 and C15 are potent biosurfactants synthetized by the Bacillus subtilis I'1a strain. The biological activity of extracted LPs was confirmed by examining extracts from I'1a cultures against uropathogenic bacteria that had been isolated from biofilms on urinary catheters. Compared with cultures of DSM 3257, which produce surfactin at a relatively low level, the extract obtained from strain I'1a exhibited a greater inhibitory effect against both planktonic and sessile forms of Escherichia coli, Serratia marcescens, Enterobacter cloacae, Proteus mirabilis, Citrobacter freundii and Enterococcus faecalis. Moreover, cyclic LP biosurfactants may disturb the integrity of cytoplasmic membranes; therefore, we investigated the effects of synthetized LPs on fatty acids and phospholipids of B. subtilis. LPs and lipids were analyzed using GC-MS, LC-MS/MS and MALDI-TOF/TOF techniques. Compared with B. subtilis DSM 3257, membranes of the I'1a strain were characterized by an increased amount of anteiso fatty acids and a ten-fold higher ratio of phosphatidylglycerol (PG)-to-phosphatidylethanolamine (PE). Interestingly, in cultures of B. subtilis DSM 3257 supplemented with LP extracts of the I'1a strain, the PG-to-PE ratio was fourfold higher, and the amount of anteiso fatty acids was also increased.
authors.affiliation: Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha Street 12/16, 90-237, Lodz, Poland. pbernat@biol.uni.lodz.pl.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Bernat
authors.affiliation: Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha Street 12/16, 90-237, Lodz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Paraszkiewicz
authors.affiliation: Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha Street 12/16, 90-237, Lodz, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Siewiera
authors.affiliation: Department of Immunobiology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, Banacha Street 12/16, 90-237, Lodz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Moryl
authors.affiliation: Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, Kossutha Street 6, 40-844, Katowice, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Płaza
authors.affiliation: Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, Kossutha Street 6, 40-844, Katowice, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Chojniak
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology; D001412:Bacillus subtilis / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects; D057785:Catheters / Q000382:microbiology*; D002853:Chromatography, Liquid; D005227:Fatty Acids / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans; D055666:Lipopeptides / Q000096:biosynthesis* / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010456:Peptides, Cyclic / Q000096:biosynthesis / Q000494:pharmacology; D010743:Phospholipids / Q000032:analysis* / Q000096:biosynthesis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014546:Urinary Catheterization / Q000009:adverse effects
chemical_list: D000900:Anti-Bacterial Agents; D005227:Fatty Acids; D055666:Lipopeptides; D010456:Peptides, Cyclic; D010743:Phospholipids; C009365:surfactin peptide; C013579:iturin A
keywords: Bacillus subtilis; Iturin; Lipidomics; Lipopeptides; Phospholipids; Uropathogens