title: 2,2',4,4'-Tetrabromodiphenyl ether injures cell viability and mitochondrial function of mouse spermatocytes by decreasing mitochondrial proteins Atp5b and Uqcrc1.
abstract: Our object was to explore direct effects and mechanism of BDE47 on GC2 (immortalized mouse spermatocyte). GC2 were exposed to DMSO, 0.1, 1, 10, 100μM BDE47 for 48h. Cell viability was detected by trypan-blue exclusion; ultrastructure by electron-microscopy; cell cycle, mitochondrial membrane motential (MMP), reactive oxygen species (ROS) by flow-cytometry; ATP production by luminometer; Atp5b, Uqcrc1, Bcl-2 level by WB. To explore whether the decreased mitochondrial proteins play an important role in apoptosis, MMP and apoptosis were detected after Atp5b or Uqcrc1 knockdown in GC2. Results showed BDE47 reduced cell viability, caused condensation of nuclear and vacuolated mitochondria, decreased MMP and ATP, induced ROS, cell cycle arrest at S and G2/M phase, reduced Atp5b, Uqcrc1, Bcl-2 in GC2. Knockdown of Atp5b or Uqcrc1 decreased MMP, induced apoptosis in GC2. Results suggested that BDE47 reduced cell viability, injured mitochondria in spermatocytes probably by decreasing mitochondrial protein Atp5b and Uqcrc1.
authors.affiliation: Department of Human Anatomy and Neuroscience, Medical School, Southeast University, Nanjing 210009, Jiangsu, China; State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu, China. Electronic address: huangsp-8888@163.com.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Huang
authors.affiliation: MingDe Hospital Affiliated with Nanjing Medical University, Nanjing 210000, Jiangsu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, Jiangsu, China.
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D014450:Electron Transport Complex III / Q000235:genetics / Q000378:metabolism*; D004785:Environmental Pollutants / Q000633:toxicity*; D055785:Gene Knockdown Techniques; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D013090:Spermatocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D004785:Environmental Pollutants; D055768:Halogenated Diphenyl Ethers; D034741:RNA, Small Interfering; C000613987:Uqcrc1 protein, mouse; C511295:2,2',4,4'-tetrabromodiphenyl ether; C530489:ATP5b protein, mouse; D025261:Mitochondrial Proton-Translocating ATPases; D014450:Electron Transport Complex III
keywords: Apoptosis; BDE47; Mitochondria; Spermatocyte; Spermatogenesis; Testis