title: Cell Fractionation and Quantitative Analysis of HIV-1 Reverse Transcription in Target Cells.
abstract: This is a protocol to detect HIV-1 reverse transcription products in cytoplasmic and nuclear fractions of cells infected with VSV-G-pseudotyped envelope-defective HIV-1. This protocol can also be extended to HIV-1 with regular envelope.
authors.affiliation: Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, USA.
authors.forename: Vaibhav B
authors.initials: VB
authors.lastname: Shah
authors.affiliation: Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Aiken