title: The c.-360 T>C mutation affects PGAM2 transcription activity and is linked with the water holding capacity of the longissimus lumborum muscle in pigs.
abstract: The phosphoglycerate mutase 2 (PGAM2) gene encodes a key enzyme in the glycolytic process. This study examined a functional mutation in the PGAM2 gene and evaluated its relationship with water holding capacity (WHC). RT-qPCR analysis showed the PGAM2 mRNA level was significantly higher in the low-WHC group than in the high-WHC group (P<0.05). The c.-360 T>C mutation was identified through sequencing and found to have opposite allele distributions in the two groups. The allele was further genotyped in 170 Duroc×Large White×Yorkshire crossbred pigs using allele-specific PCR. The CC genotype was associated with lower WHC and higher PGAM2 mRNA levels, whereas the TT genotype corresponded to a higher WHC and lower PGAM2 mRNA levels (P<0.05). A luciferase activity assay also showed that the CC-genotype promoter had higher activity than the TT-genotype promoter (P<0.05). In conclusion, we discovered the c.-360 T>C mutation in the PGAM2 gene, which is a promising marker for improving pork WHC.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Haoxin
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jiawen
authors.initials: J
authors.lastname: He
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Chu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shigang
authors.initials: S
authors.lastname: Yu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Fengyi
authors.initials: F
authors.lastname: Peng
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Hongcheng
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zengkai
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: jiechen@njau.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D009132:Muscles / Q000378:metabolism*; D009154:Mutation; D010736:Phosphoglycerate Mutase / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger; D000069466:Red Meat; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism; D014867:Water
chemical_list: D012333:RNA, Messenger; D014867:Water; D010736:Phosphoglycerate Mutase
keywords: Mutation; PGAM2; Pork; Water holding capacity