title: Association of exercise training and angiotensin-converting enzyme 2 activator improves baroreflex sensitivity of spontaneously hypertensive rats.
abstract: The present study sought to determine cardiovascular effects of aerobic training associated with diminazene aceturate (DIZE), an activator of the angiotensin converting enzyme 2, in spontaneously hypertensive rats (SHRs). Male SHRs (280-350 g) were either subjected to exercise training or not (sedentary group). The trained group was subjected to 8 weeks of aerobic training on a treadmill (five times a week, lasting 60 min at an intensity of 50-60% of maximum aerobic speed). In the last 15 days of the experimental protocol, these groups were redistributed into four groups: i) sedentary SHRs with daily treatment of 1 mg/kg DIZE (S+D1); ii) trained SHRs with daily treatment of 1 mg/kg DIZE (T+D1); iii) sedentary SHRs with daily treatment of vehicle (S+V); and iv) trained SHRs with daily treatment of vehicle (T+V). After treatment, SHRs were anesthetized and subjected to artery and femoral vein cannulation prior to the implantation of ECG electrode. After 24 h, mean arterial pressure (MAP) and heart rate (HR) were recorded; the baroreflex sensitivity and the effect of double autonomic blockade (DAB) were evaluated in non-anesthetized SHRs. DIZE treatment improved baroreflex sensitivity in the T+D1 group as compared with the T+V and S+D1 groups. The intrinsic heart rate (IHR) and MAP were reduced in T+D1 group as compared with T+V and S+D1 groups. Hence, we conclude that the association of exercise training with DIZE treatment improved baroreflex function and cardiovascular regulation.
authors.affiliation: Centro de Pesquisas em Neurociência e Fisiologia Cardiovascular, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: P R
authors.initials: PR
authors.lastname: Lopes
authors.affiliation: Centro de Pesquisas em Neurociência e Fisiologia Cardiovascular, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: M C S
authors.initials: MC
authors.lastname: Moreira
authors.affiliation: Centro de Pesquisas em Neurociência e Fisiologia Cardiovascular, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: S M
authors.initials: SM
authors.lastname: Marques
authors.affiliation: Centro de Pesquisas em Neurociência e Fisiologia Cardiovascular, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: I S J
authors.initials: IS
authors.lastname: Pinto
authors.affiliation: Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: L M
authors.initials: LM
authors.lastname: Macedo
authors.affiliation: Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: C C
authors.initials: CC
authors.lastname: Silva
authors.affiliation: Centro de Pesquisas em Neurociência e Fisiologia Cardiovascular, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: A H
authors.initials: AH
authors.lastname: Freiria-Oliveira
authors.affiliation: Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: A C S
authors.initials: AC
authors.lastname: Rebelo
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: A A S
authors.initials: AA
authors.lastname: Reis
authors.affiliation: Centro de Pesquisas em Neurociência e Fisiologia Cardiovascular, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: D A
authors.initials: DA
authors.lastname: Rosa
authors.affiliation: Faculdade de Educação Física, Universidade Federal de Uberlândia, Uberlândia, MG, Brasil.
authors.forename: M L
authors.initials: ML
authors.lastname: Ferreira-Neto
authors.affiliation: Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: C H
authors.initials: CH
authors.lastname: Castro
authors.affiliation: Centro de Pesquisas em Neurociência e Fisiologia Cardiovascular, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: G R
authors.initials: GR
authors.lastname: Pedrino
mesh_terms: D000085962:Angiotensin-Converting Enzyme 2; D000818:Animals; D017704:Baroreflex / Q000187:drug effects*; D001794:Blood Pressure / Q000502:physiology; D004133:Diminazene / Q000819:agonists / Q000031:analogs & derivatives* / Q000494:pharmacology; D006339:Heart Rate / Q000502:physiology; D006973:Hypertension / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000494:pharmacology*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D007703:Peptidyl-Dipeptidase A; C000705329:Ace2 protein, rat; D000085962:Angiotensin-Converting Enzyme 2; C003915:diminazene aceturate; D004133:Diminazene