title: Localized Movement and Levels of 53BP1 Protein Are Changed by γ-irradiation in PML Deficient Cells.
abstract: We studied epigenetics, distribution pattern, kinetics, and diffusion of proteins recruited to spontaneous and γ-radiation-induced DNA lesions. We showed that PML deficiency leads to an increased number of DNA lesions, which was accompanied by changes in histone signature. In PML wt cells, we observed two mobile fractions of 53BP1 protein with distinct diffusion in spontaneous lesions. These protein fractions were not detected in PML-deficient cells, characterized by slow-diffusion of 53BP1. Single particle tracking analysis revealed limited local motion of 53BP1 foci in PML double null cells and local motion 53BP1 foci was even more reduced after γ-irradiation. However, radiation did not change co-localization between 53BP1 nuclear bodies and interchromatin granule-associated zones (IGAZs), nuclear speckles, or chromocenters. This newly observed interaction pattern imply that 53BP1 protein could be a part of not only DNA repair, but also process mediated via components accumulated in IGAZs, nuclear speckles, or paraspeckles. Together, PML deficiency affected local motion of 53BP1 nuclear bodies and changed composition and a number of irradiation-induced foci. J. Cell. Biochem. 117: 2583-2596, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, Brno, 612 65, Czech Republic.
authors.forename: Soňa
authors.initials: S
authors.lastname: Legartová
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, Brno, 612 65, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Sehnalová
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, Brno, 612 65, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Malyšková
authors.affiliation: Department of Oral Biology, University of Oslo, Oslo, Norway.
authors.forename: Thomas
authors.initials: T
authors.lastname: Küntziger
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Norwegian Center for Stem Cell Research, Oslo, Norway.
authors.forename: Philippe
authors.initials: P
authors.lastname: Collas
authors.affiliation: Institute of Cellular Biology and Pathology, the First Faculty of Medicine, Charles University in Prague, Albertov 4, Prague, 128 01, Czech Republic.
authors.forename: Dušan
authors.initials: D
authors.lastname: Cmarko
authors.affiliation: Institute of Cellular Biology and Pathology, the First Faculty of Medicine, Charles University in Prague, Albertov 4, Prague, 128 01, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Raška
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, Brno, 612 65, Czech Republic.|Faculty of Informatics, Masaryk University, Botanická 68a, Brno, 602 00, Czech Republic.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Sorokin
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, Brno, 612 65, Czech Republic.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Kozubek
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, Brno, 612 65, Czech Republic. bartova@ibp.cz.|Institute of Cellular Biology and Pathology, the First Faculty of Medicine, Charles University in Prague, Albertov 4, Prague, 128 01, Czech Republic. bartova@ibp.cz.
authors.forename: Eva
authors.initials: E
authors.lastname: Bártová
mesh_terms: D015153:Blotting, Western; D004249:DNA Damage / Q000502:physiology* / Q000528:radiation effects; D004260:DNA Repair / Q000502:physiology* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005455:Fluorescent Antibody Technique; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D045586:Intranuclear Inclusion Bodies / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D018613:Microscopy, Confocal; D014407:Tumor Cells, Cultured; D000071857:Tumor Suppressor p53-Binding Protein 1 / Q000378:metabolism*
chemical_list: C498301:TP53BP1 protein, human; D000071857:Tumor Suppressor p53-Binding Protein 1
keywords: 53BP1 PROTEIN; DNA REPAIR; HISTONE MODIFICATIONS; MICROSCOPY; PHOTOBLEACHING; PML BODIES