title: [SIMULATION OF CHRONIC TUBERCULOSIS INFECTION IN MICE BY INTRAPERITONEAL INOCULATION AND THE TESTING OF AMINOGUANIDINE-INDUCED ACTIVATION OF RPF GENE/DELETION MUTANTS OF THE H37Rv STRAIN].
abstract: Intraperitoneal inoculation of mice with the virulent strain H37Rv of Mycobacterium tuberculosis led to the development of chronic tuberculosis for a long time. The developed model of chronic tuberculosis was used to study tuberculous infection caused by rpf gene knockout mutants of the H37Rv strain. These mutant strains were unable to produce proteins that determined the transition of quies- cent to active forms of the pathogen of tuberculosis in vitro. Unlike the wild-type M. tuberculosis H37Rv strain, the mutant ones were found to have a lower virulence and their multiplication in the organs was not stimulated by the immunosuppressant aminoguanidine. The findings prove the involvement of rpf gene products in the transition of latent (chronic) to active form of tuberculosis.
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods; D016207:Cytokines / Q000235:genetics*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology; D055786:Gene Knockout Techniques / Q000379:methods; D006146:Guanidines / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D014376:Tuberculosis / Q000382:microbiology*; D014774:Virulence / Q000187:drug effects / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D004791:Enzyme Inhibitors; D006146:Guanidines; C113704:resuscitation-promoting factor, bacteria; D019001:Nitric Oxide Synthase; C004479:pimagedine