title: Identification and Validation of Gene Expression Pattern and Signature in Patients with Immune Thrombocytopenia.
abstract: In the present study, we sought to define genes associated with immune thrombocytopenia (ITP). Microarray analysis revealed that of 1002 genes associated with ITP, 309 genes had downregulated expression and 693 genes had upregulated expression in patients with ITP. Gene set enrichment analysis revealed that 11 pathways were positively correlated to ITP, such as type I diabetes mellitus, intestinal immune network for IgA production, and oxidative phosphorylation. The messenger RNA expression levels of the indicated genes, including HLA-DRB5, IGHV3-66, IFI27, FAM212A, PLD5, tumor necrosis factor (TNF)-α, interferon-γ, interleukin (IL)-1β, and IL-4, were significantly increased in patients with ITP compared with healthy humans, while MMP8, SLC1A3, CRISP3, THBS1, FMN1, and IL-10 were decreased. In conclusion, the gene expression profile of patients with ITP has established a foundation to study the gene mechanism of ITP progression.
authors.affiliation: 1 Department of Hematology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Qi-Dong
authors.initials: QD
authors.lastname: Ye
authors.affiliation: 1 Department of Hematology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: 1 Department of Hematology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Xue-Lian
authors.initials: XL
authors.lastname: Liao
authors.affiliation: 1 Department of Hematology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.affiliation: 1 Department of Hematology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Li
mesh_terms: D000328:Adult; D016207:Cytokines / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D008297:Male; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D059467:Transcriptome / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D016207:Cytokines; C508609:IL10 protein, human; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: cytokine; gene expression profile; immune thrombocytopenia; pathway prediction