title: The role of myeloid differentiation factor 88 on mitochondrial dysfunction of peritoneal leukocytes during polymicrobial sepsis.
abstract: OBJECTIVE
  To investigate the role of myeloid differentiation factor 88 (MyD88) on mitochondrial dysfunction of peritoneal leukocytes during polymicrobial sepsis.
  
  
  METHODS
  Polymicrobial peritonitis, a clinically relevant mouse model of sepsis, was generated by cecum ligation and puncture (CLP) in both male C57BL/6J wild-type (WT) and MyD88 knockout (MyD88(-/-)) mice. Twenty-four hours after surgeries, peritoneal leukocytes were collected and four parameters of mitochondrial function, including total intracellular and mitochondrial ROS burst, mitochondrial membrane depolarization and ATP depletion, were measured by flow cytometry or ATP assay, and then compared.
  
  
  RESULTS
  Polymicrobial sepsis led to a marked mitochondrial dysfunction of peritoneal leukocytes with total intracellular and mitochondrial ROS overproduction, decreased mitochondrial membrane potential and reduced intracellular ATP production. In comparison, there was no significant difference in the extent of mitochondrial dysfunction of peritoneal leukocytes between WT and MyD88(-/-) septic mice.
  
  
  CONCLUSIONS
  MyD88 may be not sufficient to regulate mitochondrial dysfunction of peritoneal leukocytes during polymicrobial sepsis.
authors.affiliation: Biomedicine Research Center and Department of Surgery, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, China; Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Zou
authors.affiliation: Key Laboratory for Major Obstetric Diseases of Guangdong Province, Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, China.
authors.forename: Dunjin
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Chao
keywords: MyD88; mitochondrial dysfunction; peritoneal leukocyte; polymicrobial sepsis