title: miR-613 suppresses ischemia-reperfusion-induced cardiomyocyte apoptosis by targeting the programmed cell death 10 gene.
abstract: MicroRNAs (miRNAs) are important gene regulators in both biological and pathological processes, including myocardial ischemia/reperfusion (I/R) injury. This study investigated the effect of miR-613 on I/R-induced cardiomyocyte apoptosis and its molecular mechanism of action. Hypoxia/reoxygenation (H/R) significantly increased the release of lactate dehydrogenase (LDH), levels of malondialdehyde (MDA), and cardiomyocyte apoptosis, but these effects were attenuated by an miR-613 mimic. Programmed cell death 10 (PDCD10) was identified as a target gene of miR-613. miR-613 significantly increased the phosphorylation of Akt (p-Akt). An miR-613 mimic lowered the level of expression of pro-apoptotic proteins, C/EBP homologous protein (CHOP), and phosphorylated c-Jun N-terminal kinase (p-JNK), and it up-regulated the expression of the anti-apoptotic protein B-cell lymphoma-2 (Bcl-2). All of these effects were reversed by restoration of PDCD10. Taken together, the current findings indicate that miR-613 inhibits I/R-induced cardiomyocyte apoptosis by targeting PDCD10 by regulating the PI3K/AKT signaling pathway.
authors.affiliation: ICU, Departmentof Cardiac Surgery, Tianjin Chest Hospital.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wu
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Qi
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Guo
authors.forename: Peijun
authors.initials: P
authors.lastname: Li
authors.forename: Ding
authors.initials: D
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002460:Cell Line; D005786:Gene Expression Regulation; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D035683:MicroRNAs / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt; D015427:Reperfusion Injury / Q000235:genetics*; D015398:Signal Transduction
chemical_list: D051017:Apoptosis Regulatory Proteins; D008565:Membrane Proteins; D035683:MicroRNAs; C495390:PDCD10 protein, human; D011518:Proto-Oncogene Proteins; D051057:Proto-Oncogene Proteins c-akt