title: Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice.
abstract: Abnormal expression of CD200/CD200R1 may contribute to the immunologic abnormalities in patients with systemic lupus erythematosus (SLE). This study aimed to assess the function of CD200/CD200R1and impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice. Female NZB/WF1 mice were treated with CD200-Fc or control for 4 weeks. Plasma samples were collected to measure autoantibody levels. The expression levels of CD200/CD200R1 in peripheral blood mononuclear cells (PBMCs) and splenocytes were examined. The percentage of CD200/CD200R1-positive cells in splenocytes from NZB/WF1 mice was lower than that of C57BL/6 mice (p < 0.05). The plasma level of anti-dsDNA was significantly higher in NZB/WF1 mice than C57BL/6 mice (p < 0.001). However, the anti-dsDNA levels decreased (p = 0.047) after CD200-Fc treatment. Finally, CD200-Fc reduced the levels of IL-6 (p = 0.017) and IL-10 (p = 0.03) in the dendritic cell culture supernatant. This study suggests that the immunosuppressive CD200/CD200R1 signaling pathway might be involved in the immunopathology of NZB/WF1 mice; the present results merit further exploration of agents that can modulate the CD200/CD200FR1 pathway as a therapy for human lupus.
authors.affiliation: Department of Rheumatology &Clinical Immunology, Key Laboratory of Rheumatology &Clinical Immunology, Ministry of Education, Peking Union Medical College Hospital; Clinical Immunology Center, Chinese Academy of Medical Science, Beijing, 100730 China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Rheumatology &Clinical Immunology, Key Laboratory of Rheumatology &Clinical Immunology, Ministry of Education, Peking Union Medical College Hospital; Clinical Immunology Center, Chinese Academy of Medical Science, Beijing, 100730 China.
authors.forename: Lidan
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Rheumatology &Clinical Immunology, Key Laboratory of Rheumatology &Clinical Immunology, Ministry of Education, Peking Union Medical College Hospital; Clinical Immunology Center, Chinese Academy of Medical Science, Beijing, 100730 China.
authors.forename: Fengchun
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Rheumatology &Clinical Immunology, Key Laboratory of Rheumatology &Clinical Immunology, Ministry of Education, Peking Union Medical College Hospital; Clinical Immunology Center, Chinese Academy of Medical Science, Beijing, 100730 China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology* / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D007141:Immunoglobulin Fc Fragments / Q000008:administration & dosage* / Q000494:pharmacology; D007155:Immunologic Factors / Q000008:administration & dosage* / Q000494:pharmacology; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008814:Mice, Inbred NZB; D064446:Orexin Receptors; D011956:Receptors, Cell Surface / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; C477337:CD200R1 protein, human; C556062:IL10 protein, mouse; D007141:Immunoglobulin Fc Fragments; D007155:Immunologic Factors; D015850:Interleukin-6; D064446:Orexin Receptors; D011956:Receptors, Cell Surface; C577686:interleukin-6, mouse; D016753:Interleukin-10; C042739:antigens, CD200