title: Altered phosphatidylcholines expression in sputum for diagnosis of non-small cell lung cancer.
abstract: Non-small cell lung cancer (NSCLC) is a leading cause of cancer mortality worldwide, and early diagnosis needs to be improved. We examined whether neutral desorption extractive electrospray ionization mass spectrometry (ND-EESI-MS) could be used to detect sputum lipids expression changes to enable earlier diagnosis. Overall, 167 NSCLC patients and 140 controls were enrolled. The main peaks in the sputum of patients with NSCLC patients differed from controls (83.3% of total variability), and the signals were not associated with pathological type, TNM stage or smoking history. The relative abundance of peaks at m/z734, m/ z756, m/z772, m/z782, m/z798 and m/z803 reliably distinguished NSCLC sputum from control. Collision-induced dissociation confirmed that m/z734, m/z756, and m/ z772 represented [DPPC + H]+, [DPPC + Na]+, and [DPPC + K]+, respectively, and m/ z782, m/z798, and m/z803 represented sphingomyelin, phosphatidylglycerol, and phosphatidylglycerolphosphate, respectively. The relative abundance of DPPC was clearly lower in NSCLC sputum than in control, and the relative abundances of phosphatidylglycerol and phosphatidylglycerolphosphate were higher in NSCLC sputum than in control. The detection of changes in sputum lipids with ND-EESI-MS may be a noninvasive, radiation-free, relatively inexpensive, repeatable, and efficient method for diagnosis of NSCLC.
authors.affiliation: Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, P. R. China.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, P. R. China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, Jiangxi Province 330013, P. R. China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Lu
authors.affiliation: Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, Jiangxi Province 330013, P. R. China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Ding
authors.affiliation: Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, P. R. China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Yu
authors.affiliation: Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, Jiangxi Province 330013, P. R. China.
authors.forename: Penghui
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, P. R. China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, Jiangxi Province 330013, P. R. China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang, Jiangxi Province 330013, P. R. China.
authors.forename: Huanwen
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, P. R. China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008175:Lung Neoplasms / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D025341:Principal Component Analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D013109:Sphingomyelins / Q000737:chemistry; D013183:Sputum / Q000737:chemistry*
chemical_list: D008055:Lipids; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D013109:Sphingomyelins; C034358:phosphatidylglycerophosphate
keywords: NSCLC; phosphatidylcholines; sputum; ND-EESI-MS