title: Hydrogen Sulfide Protects against Chronic Unpredictable Mild Stress-Induced Oxidative Stress in Hippocampus by Upregulation of BDNF-TrkB Pathway.
abstract: Chronic unpredictable mild stress (CUMS) induces hippocampal oxidative stress. H2S functions as a neuroprotectant against oxidative stress in brain. We have previously shown the upregulatory effect of H2S on BDNF protein expression in the hippocampus of rats. Therefore, we hypothesized that H2S prevents CUMS-generated oxidative stress by upregulation of BDNF-TrkB pathway. We showed that NaHS (0.03 or 0.1 mmol/kg/day) ameliorates the level of hippocampal oxidative stress, including reduced levels of malondialdehyde (MDA) and 4-hydroxy-2-trans-nonenal (4-HNE), as well as increased level of glutathione (GSH) and activity of superoxide dismutase (SOD) in the hippocampus of CUMS-treated rats. We also found that H2S upregulated the level of BDNF and p-TrkB protein in the hippocampus of CUMS rats. Furthermore, inhibition of BDNF signaling by K252a, an inhibitor of the BDNF receptor TrkB, blocked the antioxidant effects of H2S on CUMS-induced hippocampal oxidative stress. These results reveal the inhibitory role of H2S in CUMS-induced hippocampal oxidative stress, which is through upregulation of BDNF/TrkB pathway.
authors.affiliation: Department of Neurology, Nanhua Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China; Institute of Neuroscience, Medical College, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Min
authors.identifier: 0000-0002-0989-0797
authors.initials: M
authors.lastname: Hu
authors.affiliation: Department of Neurology, Nanhua Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zou
authors.affiliation: Department of Pathophysiology, Medical College, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Wang
authors.affiliation: Department of Anatomy, Medical College, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Xi
authors.identifier: 0000-0002-1008-6237
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Neurology, Nanhua Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China; Institute of Neuroscience, Medical College, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Tan
authors.affiliation: Department of Neurology, Nanhua Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China; Institute of Neuroscience, Medical College, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Zeng
authors.affiliation: Department of Neurology, Nanhua Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Institute of Neuroscience, Medical College, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Hong-Feng
authors.initials: HF
authors.lastname: Gu
authors.affiliation: Department of Neurology, Nanhua Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China; Institute of Neuroscience, Medical College, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Xiao-Qing
authors.identifier: 0000-0002-3518-9275
authors.initials: XQ
authors.lastname: Tang
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism* / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020813:Receptor, trkB / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects* / Q000502:physiology; D013440:Sulfides / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D013440:Sulfides; D020813:Receptor, trkB; C025451:sodium bisulfide; D006862:Hydrogen Sulfide