title: Hierarchical Internal Structures in Gelatin-Bovine Serum Albumin/β-Lactoglobulin Gels and Coacervates.
abstract: Herein, we report the comparative study of gels and complex coacervates of bovine serum albumin (BSA) and beta-lactoglobulin (β-Lg) with gelatin close to their common pI. Surface patch binding produced a range of new soft matter phases (interpolymer complexes) such as opaque coacervates (charge neutralized complexes) and transparent gels (overcharged complexes). We emphasize on the comparative study of the microstructure of coacervates and gels formed at different mixing ratios using small angle scattering (SANS) data. It was found that phase states were entirely defined by the mixing ratio r = [GB]:[β-Lg or BSA]. Thermo-viscoelastic profiles of aforesaid samples revealed a smaller storage modulus and lower melting temperature for coacervates compared to gels. Thermally activated samples generated additional phases that were also probed by SANS and rheology. Thus, it is established that intermolecular association between globular proteins and a random coil polypeptide can generate various soft matter states that may facilitate harvesting of novel biomaterials.
authors.affiliation: School of Physical Sciences, Jawaharlal Nehru University , New Delhi 110067, India.
authors.forename: Jyotsana
authors.initials: J
authors.lastname: Pathak
authors.affiliation: Special Center for Nanosciences, Jawaharlal Nehru University , New Delhi 110067, India.|Inter University Accelerator Centre , New Delhi 110067, India.
authors.forename: Kamla
authors.initials: K
authors.lastname: Rawat
authors.affiliation: Solid State Physics Division, Bhabha Atomic Research Centre , Mumbai 400 085, India.
authors.forename: V K
authors.initials: VK
authors.lastname: Aswal
authors.affiliation: School of Physical Sciences, Jawaharlal Nehru University , New Delhi 110067, India.|Special Center for Nanosciences, Jawaharlal Nehru University , New Delhi 110067, India.
authors.forename: H B
authors.initials: HB
authors.lastname: Bohidar
mesh_terms: D000818:Animals; D002417:Cattle; D005780:Gelatin / Q000737:chemistry*; D005782:Gels / Q000737:chemistry; D007782:Lactoglobulins / Q000737:chemistry*; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D005782:Gels; D007782:Lactoglobulins; D012710:Serum Albumin, Bovine; D005780:Gelatin