title: MiniSOG-mediated Photoablation inCaenorhabdtis elegans.
abstract: This protocol describes a method for light-inducible cell ablation in live worms. miniSOG (mini Singlet Oxygen Generator) generates singlet oxygen upon blue light illumination (Shuet al.,2011). Mitochondrially membrane targeted miniSOG (the first 55 a. a. ofC. e.tomm-20 fused at the N'-terminus of miniSOG, termed as mito-miniSOG in the following) is transgenically expressed in specific cells/tissues (Qiet al., 2012). Groups of transgenic animals are illuminated under open field fluorescence light on a compound microscope or LED light setup for photo-ablation.
authors.affiliation: Division of Biological Sciences, Neurobiology Section, University of California, San Diego, CA, USA.
authors.forename: Yingchuan
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Division of Biological Sciences, Neurobiology Section, University of California, San Diego, CA, USA.
authors.forename: Suhong
authors.initials: S
authors.lastname: Xu