title: Exploring the Shift in Structure and Function of Microbial Communities Performing Biological Phosphorus Removal.
abstract: A sequencing batch reactor fed mainly by acetate was operated to perform enhanced biological phosphorus removal (EBPR). A short-term pH shock from 7.0 to 6.0 led to a complete loss of phosphate-removing capability and a drastic change of microbial communities. 16S rRNA gene pyrosequencing showed that large proportions of glycogen accumulating organisms (GAOs) (accounted for 16% of bacteria) bloomed, including Candidatus Competibacter phosphatis and Defluviicoccus-related tetrad-forming organism, causing deteriorated EBPR performance. The EBPR performance recovered with time and the dominant Candidatus Accumulibacter (Accumulibacter) clades shifted from Clade IIC to IIA while GAOs populations shrank significantly. The Accumulibacter population variation provided a good opportunity for genome binning using a bi-dimensional coverage method, and a genome of Accumulibacter Clade IIC was well retrieved with over 90% completeness. Comparative genomic analysis demonstrated that Accumulibacter clades had different abilities in nitrogen metabolism and carbon fixation, which shed light on enriching different Accumulibacter populations selectively.
authors.affiliation: College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, China.|Environmental Biotechnology Laboratory, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Environmental Biotechnology Laboratory, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Environmental Biotechnology Laboratory, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Liguan
authors.initials: L
authors.lastname: Li
authors.affiliation: Environmental Biotechnology Laboratory, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Jiang
authors.affiliation: School of Environment, Nanjing University, Nanjing, China.
authors.forename: Xuxiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Environment, Nanjing University, Nanjing, China.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Ren
authors.affiliation: Environmental Biotechnology Laboratory, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D057486:Carbon Cycle / Q000235:genetics; D016680:Genome, Bacterial; D006801:Humans; D006863:Hydrogen-Ion Concentration; D059013:Microbial Consortia / Q000235:genetics*; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D062065:Wastewater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*; D018508:Water Purification / Q000379:methods
chemical_list: D009584:Nitrogen; D010758:Phosphorus; D012336:RNA, Ribosomal, 16S; D062065:Wastewater; D014874:Water Pollutants, Chemical