title: Marinibaculum pumilum gen. nov., sp. nov., isolated from seawater.
abstract: A Gram-stain-negative, facultatively anaerobic, motile and rod-shaped strain, designed H2T, was isolated from the Western Pacific Ocean, and subjected to a taxonomic investigation using a polyphasic approach. Strain H2T grew at 15-40 °C and pH 6.0-9.0 (optimum 37 °C and pH 6.5), and with 1-10 % (w/v) NaCl (optimum 2 %). The predominant respiratory quinone was ubiquinone-10 (Q-10) and the major fatty acids identified were C19 : 0 cyclo ω8c, C18 : 1ω7c, C18 : 0 and 11-methyl-C18 : 1ω7c. The polar lipids of strain H2T consisted of phosphatidylglycerol, one unknown phospholipid, one unknown glycolipid and three unidentified aminolipids. The DNA G+C content was 75.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain H2T formed a distinct clade belong to the family Rhodospirillaceae within the Alphaproteobacteria. On the basis of morphological, physiological and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain H2T represents a novel species in a new genus in the family Rhodospirillaceae, for which the name Marinibaculumpumilum gen. nov., sp. nov. is proposed. The type strain of the type species is H2T(=MCCC 1K02279T=KCTC 42964T).
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Yu
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Tian-Yi
authors.initials: TY
authors.lastname: Tao
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Ge-Yi
authors.initials: GY
authors.lastname: Fu
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Shuai-Bo
authors.initials: SB
authors.lastname: Han
authors.affiliation: Ocean College, Zhejiang University, Zhoushan 316000, PR China.
authors.forename: Rui-Jun
authors.initials: RJ
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Sun
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D010140:Pacific Ocean; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012244:Rhodospirillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2