title: A long-lasting cardiomyogenic gene expression by PEI-based transfection induces endogenous cardiac mRNAs in human adipose-derived stem cells.
abstract: Our previous work revealed that a polyethyleneimine (PEI)-based gene delivery causes robust and sustained expression of exogenous genes in human adipose-derived stem cells (hADSCs). Here we use this method to test whether a single introduction of cDNAs for the three cardiomyogenic reprogramming genes (GATA4, MEF2C, and TBX5) might be sufficient to induce transdifferentiation of hADSCs towards the cardiomyogenic lineage. A single transfection results in sustained expression of the introduced genes for more than two weeks. hADSCs exhibit undetectable or very low levels of mRNAs for endogenous GATA4, MEF2C and TBX5. However, mRNAs for these endogenous factors become apparent at ∼2 weeks after transfection and keep increasing until the end of experimental period at the fifth week. Concordant with these cardiomyogenic genes, Nkx2.5 mRNA becomes significant at ∼2 weeks and gradually increases until the end of experimental period. Several other cardiomyogenic mRNAs were also significant at 5 weeks. Thus, a single transfection of cDNAs for the cardiomyogenic reprogramming genes using a PEI-based method induces transdifferentiation of ADSCs.
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Eulsoon
authors.initials: E
authors.lastname: Park
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan. Electronic address: koichi@vos.nagaokaut.ac.jp.
authors.forename: Koichi
authors.initials: K
authors.lastname: Takimoto
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D054338:Cell Transdifferentiation / Q000235:genetics; D002478:Cells, Cultured; D050991:GATA4 Transcription Factor / Q000235:genetics; D015870:Gene Expression; D000072576:Homeobox Protein Nkx-2.5 / Q000235:genetics; D006801:Humans; D064326:MEF2 Transcription Factors / Q000235:genetics; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D011094:Polyethyleneimine / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D020825:T-Box Domain Proteins / Q000235:genetics; D013997:Time Factors; D014162:Transfection / Q000379:methods
chemical_list: D050991:GATA4 Transcription Factor; C484170:GATA4 protein, human; D000072576:Homeobox Protein Nkx-2.5; D064326:MEF2 Transcription Factors; C578040:MEF2C protein, human; C095552:NKX2-5 protein, human; D012333:RNA, Messenger; D020825:T-Box Domain Proteins; C103172:T-box transcription factor 5; D011094:Polyethyleneimine
keywords: Adipose-derived stem cells; Cardiomyocyte; Reprogramming; Transdifferentiation; Transfection