title: The Distribution of Ki-67 and Doublecortin Immunopositive Cells in the Brains of Three Microchiropteran Species, Hipposideros fuliginosus, Triaenops persicus, and Asellia tridens.
abstract: This study uses Ki-67 and doublecortin (DCX) immunohistochemistry to delineate potential neurogenic zones, migratory pathways, and terminal fields associated with adult neurogenesis in the brains of three microchiropterans. As with most mammals studied to date, the canonical subgranular and subventricular neurogenic zones were observed. Distinct labeling of newly born cells and immature neurons within the dentate gyrus of the hippocampus was observed in all species. A distinct rostral migratory stream (RMS) that appears to split around the medial aspect of the caudate nucleus was observed. These two rostral stream divisions appear to merge at the rostroventral corner of the caudate nucleus to turn and enter the olfactory bulb, where a large terminal field of immature neurons was observed. DCX immunolabeled neurons were observed mostly in the rostral neocortex, but a potential migratory stream to the neocortex was not identified. A broad swathe of newly born cells and immature neurons was found between the caudoventral division of the RMS and the piriform cortex. In addition, occasional immature neurons were observed in the amygdala and DCX-immunopositive axons were observed in the anterior commissure. While the majority of these features have been found in several mammal species, the large number of DCX immunolabeled cells found between the RMS and the piriform cortex and the presence of DCX immunostained axons in the anterior commissure are features only observed in microchiropterans and insectivores to date. In the diphyletic scenario of chiropteran evolution, these observations align the microchiropterans with the insectivores. Anat Rec, 299:1548-1560, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: School of Anatomical Sciences Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, 2193, Johannesburg, Republic of South Africa.
authors.forename: Richard
authors.initials: R
authors.lastname: Chawana
authors.affiliation: School of Anatomical Sciences Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, 2193, Johannesburg, Republic of South Africa.
authors.forename: Nina
authors.initials: N
authors.lastname: Patzke
authors.affiliation: KSU Mammals Research Chair, Department, of Zoology, College of Science, King Saud University, Box, 2455, Riyadh, 11451, Saudi Arabia.|Saudi Wildlife Authority, Riyadh, 11575, Saudi Arabia.
authors.forename: Abdulaziz N
authors.initials: AN
authors.lastname: Alagaili
authors.affiliation: KSU Mammals Research Chair, Department, of Zoology, College of Science, King Saud University, Box, 2455, Riyadh, 11451, Saudi Arabia.|Department of Zoology and Entomology, University of Pretoria, Pretoria, 0002, South Africa.
authors.forename: Nigel C
authors.initials: NC
authors.lastname: Bennett
authors.affiliation: KSU Mammals Research Chair, Department, of Zoology, College of Science, King Saud University, Box, 2455, Riyadh, 11451, Saudi Arabia.
authors.forename: Osama B
authors.initials: OB
authors.lastname: Mohammed
authors.affiliation: Faculté des Sciences, University of Kisangani, B.P. 1232, Kisangani, Democratic Republic of Congo.
authors.forename: Consolate
authors.initials: C
authors.lastname: Kaswera-Kyamakya
authors.affiliation: Department of African Zoology, Royal Museum for Central Africa, Leuvensesteenweg 13, B-3080, Tervuren, Belgium.|Laboratory of Histology and Neuropathology, Université Libre de Bruxelles, Brussels, 1070, Belgium.|Department of Anthropology, University of Arkansas, Fayetteville, Arkansas.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Gilissen
authors.affiliation: School of Anatomical Sciences Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, 2193, Johannesburg, Republic of South Africa.
authors.forename: Amadi O
authors.initials: AO
authors.lastname: Ihunwo
authors.affiliation: Queensland Brain Institute, University of Queensland, 4072, St. Lucia, Australia.
authors.forename: John D
authors.initials: JD
authors.lastname: Pettigrew
authors.affiliation: School of Anatomical Sciences Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, 2193, Johannesburg, Republic of South Africa. Paul.Manger@wits.ac.za.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Manger
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002685:Chiroptera; D000089622:Doublecortin Domain Proteins; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D058953:Neural Stem Cells / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*
chemical_list: D000089622:Doublecortin Domain Proteins; D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides
keywords: Chiroptera; Ki-67; adult neurogenesis; anterior commissure; doublecortin; hippocampus; piriform cortex; wild-living animals