title: Renal Denervation Suppresses the Inducibility of Atrial Fibrillation in a Rabbit Model for Atrial Fibrosis.
abstract: Renal denervation (RD) was reported to reduce the susceptibility of atrial fibrillation (AF), but the underlying mechanism has not been well understood. This study was performed to investigate the effect of RD on the inducibility of AF in a rabbit model for atrial fibrosis and to explore the potential mechanisms. Thirty-five rabbits were randomly assigned into sham-operated group (n = 12), abdominal aortic constriction (AAC) group (n = 12) and AAC with RD (AAC-RD) group (n = 11). The incidence of AF induced by burst pacing in atriums was determined. Blood was collected to measure the levels of rennin, angiotensin II and aldosterone. Atrial samples were preserved to evaluate protein and gene expression of collagen, connective tissue growth factor (CTGF) and transforming growth factor-β1 (TGF-β1). Our data suggested cardiac structure remodeling and atrial fibrosis were successfully induced by AAC. Compared with the AAC group, the AAC-RD rabbits had smaller ascending aortic diameter and left ventricular end-systolic diameter. For burst pacing at the left atrium (LA), AF was induced in two of the 12 rabbits in the sham-operated group, 10 of the 12 rabbits in the AAC group, and 2 of the 11 rabbits in the AAC-RD group, with great difference among the three groups (P = 0.001). The percentage of LA burst stimulations with induced AF achieved 47.2% in the AAC group, which was higher than those in both the AAC-RD (12.1%) and the Sham-operated (5.6%) groups. Significantly increasing intercellular space in the AAC group (P<0.001) compared with the sham-operated rabbits. RD clearly decreased the volume fraction of collagen in LA and right atrium compared with that of the AAC group (P< 0.01). AAC-induced elevation of collagen I, CTGF and TGF-β1 was suppressed by RD. In conclusion, RD suppressed the inducibility of AF in a rabbit model for pressure associated atrial fibrosis, potentially by modulating renin-angiotensin-aldosterone system and decreasing pro-fibrotic factors.
authors.affiliation: Department of cardiology, Shanghai Songjiang Central Hospital, Shanghai, 201600, China.|Department of cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200800, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200800, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200800, China.
authors.forename: Genqing
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Department of cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200800, China.
authors.forename: Songwen
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of cardiology, Shanghai Songjiang Central Hospital, Shanghai, 201600, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ouyang
authors.affiliation: Department of cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200800, China.
authors.forename: Shaowen
authors.initials: S
authors.lastname: Liu
mesh_terms: D000450:Aldosterone / Q000378:metabolism; D000818:Animals; D001281:Atrial Fibrillation / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology; D003094:Collagen / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D003714:Denervation / Q000009:adverse effects*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006325:Heart Atria / Q000378:metabolism; D007668:Kidney / Q000294:innervation*; D008297:Male; D011817:Rabbits; D012084:Renin-Angiotensin System; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor; D000450:Aldosterone; D003094:Collagen