title: [Influence of S1PR5 Defect on the Lymphocyte Distribution in Mice].
abstract: BACKGROUND
  The sphingosine 1-phosphate (S1P) receptors (S1PRs) are a group of G protein-coupled receptors expressed on the surface of lymphocytes. The interaction between S1P and S1PRs plays a significant role in the migration and distribution of lymphocytes.
  
  
  OBJECTIVE
  To investigate the influence of S1PR5 defect on the lymphocytes distribution in mice.
  
  
  METHODS
  The distribution of different subsets of lymphocyte in the mice with S1PR5 defect was examined by flow cytometry.
  
  
  RESULTS
  Compared with wild type mice, the number of NK cells in the peripheral blood (PB) and spleen (SP) from the mice with S1PR5 defect decreased very significantly 〔PB: 6.4±0.45% vs 2.2±0.47(P<0.01,n=3)；SP: 3.0±0.91% vs 0.68±0.14%(P<0.05,n=3)〕. However, the NK cell number in the bone marrow (BM) and lymphonodes (LN) of the mice with S1PR5 defect increased very significantly 〔BM: 0.97±0.20 % vs 2.6±0.35% (P<0.01, n=3); LN: 0.35±0.16% vs 1.7±0.15% (P<0.01, n=3)〕. The percentages of CD3(+) lymphocyte in peripheral blood, spleen and lymph node were not statistically significantly different between these 2 types of mice 〔PB: 17.3±7.9% vs 17.0±4.6% (P>0.05, n=3); SP: 33.0±6.0% vs 27.4±1.8% (P>0.05, n=3); LN: 42.3±10.7% vs 51.2±2.7% (P>0.05, n=3)〕.
  
  
  CONCLUSIONS
  S1PR5 defect can significantly influence the NK cell distribution.
authors.affiliation: Department of Hematology, Chinese PLA Medical School & Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Zhen-Yang
authors.initials: ZY
authors.lastname: Gu
authors.affiliation: Department of Hematology, Chinese PLA Medical School & Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: Department of Hematology, Chinese PLA Medical School & Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Yan
authors.affiliation: Department of Hematology, Chinese PLA Medical School & Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Hematology, Chinese PLA Medical School & Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Fei-Yang
authors.initials: FY
authors.lastname: Wang
authors.affiliation: Department of Hematology, Chinese PLA Medical School & Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Department of Hematology, Chinese PLA Medical School & Chinese PLA General Hospital, Beijing 100853, China.E-mail: gaochunji@hotmail.com.
authors.forename: Chun-Ji
authors.initials: CJ
authors.lastname: Gao
mesh_terms: D000818:Animals; D001853:Bone Marrow; D002452:Cell Count; D005434:Flow Cytometry; D008214:Lymphocytes; D008246:Lysophospholipids; D051379:Mice; D049349:Receptors, Lysosphingolipid; D013110:Sphingosine / Q000031:analogs & derivatives
chemical_list: D008246:Lysophospholipids; D049349:Receptors, Lysosphingolipid; C060506:sphingosine 1-phosphate; D013110:Sphingosine