title: Green Tea Polyphenols Alleviate Autophagy Inhibition Induced by High Glucose in Endothelial Cells.
abstract: Bovine aortic endothelial cells (BAECs) were cultured with high glucose (33 mmol/L), 4 mg/L green tea polyphenols (GTPs) or 4 mg/L GTPs co-treatment with high glucose for 24 h in the presence or absence of Bafilomycin-A1 (BAF). We observed that high glucose increased the accumulation of LC3-II. Treatment with BAF did not further increase the accumulation of LC3-II. Results also showed an increased level of p62 and decreased Beclin-1. However, GTPs showed inversed trends of those proteins. Furthermore, GTPs co-treatment with high glucose decreased the level of LC3-II and a much higher accumulation of LC3-II was observed in the presence of BAF in comparison with high glucose alone. Results also showed a decreased p62 and increased Beclin-1. The results demonstrated that GTPs alleviated autophagy inhibition induced by high glucose, which may be involved in the endothelial protective effects of green tea against hyperglycemia.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
authors.forename: Pi Wei
authors.initials: PW
authors.lastname: Zhang
authors.affiliation: School of Nursing, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Tian
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
authors.forename: Fang Yi
authors.initials: FY
authors.lastname: Xu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
authors.forename: Raynard
authors.initials: R
authors.lastname: Burnside
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
authors.forename: Wei Jie
authors.initials: WJ
authors.lastname: Yi
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
authors.forename: Si Yun
authors.initials: SY
authors.lastname: Xiang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
authors.forename: Nan Nan
authors.initials: NN
authors.lastname: Wu
authors.affiliation: Kecheng People's Hospital, Quzhou 324000, Zhejiang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: School of Environmental Science and Public Health, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China.
authors.forename: Na Na
authors.initials: NN
authors.lastname: Zhao
authors.affiliation: School of Environmental Science and Public Health, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China.
authors.forename: Xiao Lei
authors.initials: XL
authors.lastname: Ye
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
authors.forename: Chen Jiang
authors.initials: CJ
authors.lastname: Ying
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D002417:Cattle; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000633:toxicity*; D018942:Macrolides / Q000494:pharmacology; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry*
chemical_list: D018942:Macrolides; D059808:Polyphenols; D013662:Tea; C040929:bafilomycin A1; D005947:Glucose