title: [Expression Profile of lncRNA NONHSAT040475 in Peripheral Blood Leukocytes of Healthy Persons].
abstract: OBJECTIVE
  To investigate the expression profile of lncRNA NONHSAT040475 in peripheral blood leukocytes of healthy persons.
  
  
  METHODS
  The peripheral blood mononuclear cells were collected from 10 healthy volunteers, the CD3(+) T cells,CD19(+) B cells,CD56(+) NK cells and granulocytes were purified and sorted by flow cytometry. Then, the expression of lncRNA NONHSAT040475 in peripheral blood leukocyte subsets was detected by real-time quantitative PCR.
  
  
  RESULTS
  the expression levels of lncRNA NONHSAT040475 were various in lymphocyte subsets, its expression in B lymphocytes was significantly higher, as compared with T lymphocytes, while the expressions of lncRNA NONHSAT040475 in NK cells and granulocytes were relative low（P<0.01）.
  
  
  CONCLUSIONS
  lncRNA NONHSAT040475 is widely expressed in human peripheral blood leukocytes, and mainly expressed in B lymphocytes, therefore, laying the foundation for further study of B lymphocyte-related diseases. This study has brought a new opportunity for diagnosing and illustrating the molecular mechanism of hematologic disorder or autoimmune disease.
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Medical College of Chinese PLA, Beijing 100853, China.
authors.forename: Fei-Yan
authors.initials: FY
authors.lastname: Wang
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Medical College of Chinese PLA, Beijing 100853, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Medical College of Chinese PLA, Beijing 100853, China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Zhao
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Medical College of Chinese PLA, Beijing 100853, China.
authors.forename: Hua-Ping
authors.initials: HP
authors.lastname: Wei
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Medical College of Chinese PLA, Beijing 100853, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Medical College of Chinese PLA, Beijing 100853, China.
authors.forename: Zhen-Yang
authors.initials: ZY
authors.lastname: Gu
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Medical College of Chinese PLA, Beijing 100853, China.
authors.forename: Li-Xun
authors.initials: LX
authors.lastname: Guan
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Medical College of Chinese PLA, Beijing 100853, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Medical College of Chinese PLA, Beijing 100853, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Medical College of Chinese PLA, Beijing 100853, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Medical College of Chinese PLA, Beijing 100853, China.E-mail:gaochunji@hotmail.com.
authors.forename: Chun-Ji
authors.initials: CJ
authors.lastname: Gao
mesh_terms: D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural; D007958:Leukocyte Count; D007962:Leukocytes; D062085:RNA, Long Noncoding
chemical_list: D062085:RNA, Long Noncoding