title: Magnetite-Binding Flagellar Filaments Displaying the MamI Loop Motif.
abstract: This work aimed at developing a novel method for fabricating 1 D magnetite nanostructures with the help of mutated flagellar filaments. We constructed four different flagellin mutants displaying magnetite-binding motifs: two contained fragments of magnetosome-associated proteins from magnetotactic bacteria (MamI and Mms6), and synthetic sequences were used for the other two. A magnetic selection method identified the MamI mutant as having the highest binding affinity to magnetite. Filaments built from MamI loop-containing flagellin subunits were used as templates to form chains of magnetite nanoparticles along the filament by capturing them from suspension. Our study represents a proof-of-concept that flagellar filaments can be engineered to facilitate formation of 1 D magnetite nanostructures under ambient conditions. In addition, it proves the interaction between MamI and magnetite, with implications for the role of this protein in magnetotactic bacteria.
authors.affiliation: Department of Earth and Environmental Sciences, University of Pannonia, Egyetem u. 10, 8200, Veszprém, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Bereczk-Tompa
authors.affiliation: Department of Earth and Environmental Sciences, University of Pannonia, Egyetem u. 10, 8200, Veszprém, Hungary.
authors.forename: Mihály
authors.identifier: 0000-0001-9355-3533
authors.initials: M
authors.lastname: Pósfai
authors.affiliation: Bio-Nanosystems Laboratory, Research Institute for Biomolecular and Chemical Engineering, University of Pannonia, Egyetem u. 10, 8200, Veszprém, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Tóth
authors.affiliation: Bio-Nanosystems Laboratory, Research Institute for Biomolecular and Chemical Engineering, University of Pannonia, Egyetem u. 10, 8200, Veszprém, Hungary. von007@almos.vein.hu.|Institute of Technical Physics and Materials Science, Centre for Energy Research, Konkoly-Thege u. 29-33, 1121, Budapest, Hungary. von007@almos.vein.hu.
authors.forename: Ferenc
authors.identifier: 0000-0002-6498-8172
authors.initials: F
authors.lastname: Vonderviszt
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D005407:Flagella / Q000737:chemistry* / Q000378:metabolism; D005408:Flagellin / Q000235:genetics / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D055944:Magnetosomes / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular
chemical_list: D001426:Bacterial Proteins; D058185:Magnetite Nanoparticles; D005408:Flagellin
keywords: MamI protein; biomimetic synthesis; flagellar filament; magnetic nanofiber; template synthesis