title: Inhalation of water electrolysis-derived hydrogen ameliorates cerebral ischemia-reperfusion injury in rats - A possible new hydrogen resource for clinical use.
abstract: Hydrogen is a kind of noble gas with the character to selectively neutralize reactive oxygen species. Former researches proved that low-concentration of hydrogen can be used to ameliorating cerebral ischemia/reperfusion injury. Hydrogen electrolyzed from water has a hydrogen concentration of 66.7%, which is much higher than that used in previous studies. And water electrolysis is a potential new hydrogen resource for regular clinical use. This study was designed and carried out for the determination of safety and neuroprotective effects of water electrolysis-derived hydrogen. Sprague-Dawley rats were used as experimental animals, and middle cerebral artery occlusion was used to make cerebral ischemia/reperfusion model. Pathologically, tissues from rats in hydrogen inhalation group showed no significant difference compared with the control group in HE staining pictures. The blood biochemical findings matched the HE staining result. TTC, Nissl, and TUNEL staining showed the significant improvement of infarction volume, neuron morphology, and neuron apoptosis in rat with hydrogen treatment. Biochemically, hydrogen inhalation decreased brain caspase-3, 3-nitrotyrosine and 8-hydroxy-2-deoxyguanosine-positive cells and inflammation factors concentration. Water electrolysis-derived hydrogen inhalation had neuroprotective effects on cerebral ischemia/reperfusion injury in rats with the effect of suppressing oxidative stress and inflammation, and it is a possible new hydrogen resource to electrolyze water at the bedside clinically.
authors.affiliation: Graduate Management Unit, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Orthopedics, Changhai hospital Affiliated to the Second Military Medical University, Shanghai, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Graduate Management Unit, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhai
authors.affiliation: Graduate Management Unit, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, PR China.
authors.forename: Dongchen
authors.initials: D
authors.lastname: Shi
authors.affiliation: Department of Naval Aviation Medicine, The Second Military Medical University, Shanghai, PR China.
authors.forename: Rongjia
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Graduate Management Unit, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhi
authors.affiliation: Graduate Management Unit, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, PR China.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Changhai hospital Affiliated to the Second Military Medical University, Shanghai, PR China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Department of Orthopedics, Changhai hospital Affiliated to the Second Military Medical University, Shanghai, PR China.
authors.forename: Liehu
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Orthopedics, Changhai hospital Affiliated to the Second Military Medical University, Shanghai, PR China.
authors.forename: Weizong
authors.initials: W
authors.lastname: Weng
authors.affiliation: Department of Orthopedics, Changhai hospital Affiliated to the Second Military Medical University, Shanghai, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Fuzhou General Hospital of PLA, Fuzhou, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Naval Aviation Medicine, The Second Military Medical University, Shanghai, PR China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Orthopedics, Changhai hospital Affiliated to the Second Military Medical University, Shanghai, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ji
authors.affiliation: Department of Anesthesiology, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, PR China. Electronic address: Houjiong@126.com.
authors.forename: Jiong
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Orthopedics, Changhai hospital Affiliated to the Second Military Medical University, Shanghai, PR China. Electronic address: Jiacansu@126.com.
authors.forename: Jiacan
authors.initials: J
authors.lastname: Su
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D002545:Brain Ischemia / Q000188:drug therapy / Q000378:metabolism*; D006859:Hydrogen / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy*; D014867:Water
chemical_list: D018696:Neuroprotective Agents; D014867:Water; D006859:Hydrogen
keywords: hydrogen gas; inflammation; ischemia–reperfusion injury; new hydrogen resource; oxidative stress; water electrolysis