title: Biophysical characterization data of the artificial protein Octarellin V.1 and binding test with its X-ray helpers.
abstract: The artificial protein Octarellin V.1 (http://dx.doi.org/10.1016/j.jsb.2016.05.004[1]) was obtained through a direct evolution process over the de novo designed Octarellin V (http://dx.doi.org/10.1016/S0022-2836(02)01206-8[2]). The protein has been characterized by circular dichroism and fluorescence techniques, in order to obtain data related to its thermo and chemical stability. Moreover, the data for the secondary structure content studied by circular dichroism and infra red techniques is reported for the Octarellin V and V.1. Two crystallization helpers, nanobodies (http://dx.doi.org/10.1038/nprot.2014.039[3]) and αRep (http://dx.doi.org/10.1016/j.jmb.2010.09.048[4]), have been used to create stable complexes. Here we present the data obtained of the binding characterization of the Octarellin V.1 with the crystallization helpers by isothermal titration calorimetry.
authors.affiliation: GIGA-Research, Molecular Biomimetics and Protein Engineering, University of Liège, Liège, Belgium; Biochemistry and Molecular Biology Department, University of Concepcion, Concepción, Chile.
authors.forename: Maximiliano
authors.initials: M
authors.lastname: Figueroa
authors.affiliation: Laboratoire d'Enzymologie et Repliement des Protéines, Centre for Protein Engineering, University of Liège, Liège, Belgium.
authors.forename: Julie
authors.initials: J
authors.lastname: Vandenameele
authors.affiliation: Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Erik
authors.initials: E
authors.lastname: Goormaghtigh
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), UMT 9198, CEA, CNRS, Université Paris-Sud, Orsay, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Valerio-Lepiniec
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), UMT 9198, CEA, CNRS, Université Paris-Sud, Orsay, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Minard
authors.affiliation: Laboratoire d'Enzymologie et Repliement des Protéines, Centre for Protein Engineering, University of Liège, Liège, Belgium.
authors.forename: André
authors.initials: A
authors.lastname: Matagne
authors.affiliation: GIGA-Research, Molecular Biomimetics and Protein Engineering, University of Liège, Liège, Belgium.
authors.forename: Cécile
authors.initials: C
authors.lastname: Van de Weerdt
keywords: Artificial proteins; Circular dichroism; Crystallization helpers; Infra red spectroscopy; Isothermal Titration Calorimetry; Protein design