title: A magnetite suspension-based washing method for immunoassays using Escherichia coli cells with autodisplayed Z-domains.
abstract: Escherichia coli cells with autodisplayed Z-domains have been used for immunoassays of specific target analytes. In this study, a magnetite suspension was used for the washing step in immunoassays of E. coli cells with autodisplayed Z-domains. This approach enhanced the washing conditions for these immunoassays by determining (1) the optimal concentration of the magnetite suspension, (2) the capacity of the magnetite suspension-based washing method to recover E. coli cells, and (3) the level at which the activity of autodisplayed Z-domains is maintained. In immunoassays of C-reactive protein (CRP), the immunoassay incorporating the magnetite suspension-based washing method showed a sensitivity and limit of detection considerably higher than those of the conventional centrifugation-based washing method. The results indicated that immunoassays incorporating the magnetite suspension-based washing method are effective for medical diagnoses based on CRP assay.
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-749, Korea.
authors.forename: Do-Hoon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-749, Korea.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Bong
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-749, Korea.
authors.forename: Gu
authors.initials: G
authors.lastname: Yoo
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-749, Korea.
authors.forename: Seo-Yoon
authors.initials: SY
authors.lastname: Chang
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-749, Korea.
authors.forename: Young Wook
authors.initials: YW
authors.lastname: Chang
authors.affiliation: Korea Institute of Science and Technology, Seoul, Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kang
authors.affiliation: Muenster University, Muenster, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Jose
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-749, Korea. Electronic address: jcpyun@yonsei.ac.kr.
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Pyun
mesh_terms: D055910:Antibodies, Immobilized; D002097:C-Reactive Protein / Q000032:analysis; D061505:Cell Surface Display Techniques; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D052203:Ferrosoferric Oxide; D005434:Flow Cytometry; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D018189:Immunomagnetic Separation; D057230:Limit of Detection; D000072417:Protein Domains; D013535:Suspensions
chemical_list: D055910:Antibodies, Immobilized; D013535:Suspensions; D002097:C-Reactive Protein; D052203:Ferrosoferric Oxide
keywords: Autodisplay; Immunoassay; Magnetites; Washing step