title: Long Noncoding RNA UFC1 Promotes Proliferation of Chondrocyte in Osteoarthritis by Acting as a Sponge for miR-34a.
abstract: Osteoarthritis (OA) is one of the most common prevalent chronic joint diseases. Emerging pieces of evidence have demonstrated that chondrocytes survival was closely associated with the destruction of joints in OA patients. Long noncoding RNAs (lncRNAs), defined as >200 nucleotides in length, also have been implicated in a variety of disease states. However, there are few studies on the role of lncRNAs in OA, and the pathological contributions of lncRNAs to OA remain largely unknown. In this study, we examined the expression of lncRNA UFC1 in cartilage samples from OA patients and healthy subjects, and then investigated biological function of UFC1 in OA chondrocyte. We found that the UFC1 was significantly reduced in OA patients. Functional assays demonstrated that UFC1 promotes chondrocytes proliferation and inhibits cell apoptosis. Furthermore, we found that UFC1 regulates survival of OA chondrocytes through physically association with miR-34a. Taken together, our data highlight the important roles of lncRNA UFC1 in the survival of OA chondrocytes. UFC1 may be a potential therapy for OA.
authors.affiliation: 1 Department of Articular Surgery, Qianfoshan Hospital of Shandong University , Jinan, China .|2 Affiliated Hospital of Taishan Medical University , Taian, China .
authors.forename: Gang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: 3 College of Sports Medicine and Rehabilitation, Taishan Medical University , Taian, China .
authors.forename: Yadi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: 2 Affiliated Hospital of Taishan Medical University , Taian, China .
authors.forename: Dong
authors.initials: D
authors.lastname: Xu
authors.affiliation: 1 Department of Articular Surgery, Qianfoshan Hospital of Shandong University , Jinan, China .
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Yan
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics*; D002469:Cell Separation / Q000379:methods; D019902:Chondrocytes / Q000166:cytology*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D010003:Osteoarthritis / Q000235:genetics* / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes / Q000378:metabolism*
chemical_list: C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; C502096:UFC1 protein, human; D044763:Ubiquitin-Conjugating Enzymes
keywords: UFC1; lncRNA; miR-34a; osteoarthritis