title: An insight into anti-biofilm and anti-quorum sensing activities of the selected anthocyanidins: the case study of Pseudomonas aeruginosa PAO1.
abstract: Anti-biofilm activity of three anthocyanidins (pelargonidin, cyanidin and delphinidin) was evaluated for the first time at in vitro conditions. All the compounds reduced the formation of Pseudomonas aeruginosa PAO1 biofilm at low sub-MIC (0.125 MIC) with delphinidin (c 56.25 μg/mL) being the most active (43%). In comparison, ampicillin (c 93.75 μg/mL) and streptomycin (c 21.25 μg/mL) (used as positive controls) were considerably less effective at the same sub-MIC (8 and 12%, respectively). Furthermore, at 0.5 MIC (c 225 μg/mL) this anthocyanidin molecule partly reduced the bacterial protrusions. However, no any of the aforementioned compounds inhibited the production of pyocyanin by the bacterial strain P. aeruginosa PAO1. Taken all together, the delphinidin scaffold could be taken into consideration for the design of the novel and more effective anti-biofilm agents inspired by the anthocyanidins.
authors.affiliation: a Department of Life Sciences, Institute for Multidisciplinary Research (IMSI) , University of Belgrade , Belgrade , Serbia.
authors.forename: Boris
authors.initials: B
authors.lastname: Pejin
authors.affiliation: b Mycological Laboratory, Department of Plant Physiology, Institute for Biological Research "Sinisa Stankovic" (IBISS) , University of Belgrade , Belgrade , Serbia.
authors.forename: Ana
authors.initials: A
authors.lastname: Ciric
authors.affiliation: c Faculty of Physical Chemistry , University of Belgrade , Belgrade , Serbia.
authors.forename: Jasmina
authors.initials: J
authors.lastname: Dimitric Markovic
authors.affiliation: b Mycological Laboratory, Department of Plant Physiology, Institute for Biological Research "Sinisa Stankovic" (IBISS) , University of Belgrade , Belgrade , Serbia.
authors.forename: Jasmina
authors.initials: J
authors.lastname: Glamoclija
authors.affiliation: b Mycological Laboratory, Department of Plant Physiology, Institute for Biological Research "Sinisa Stankovic" (IBISS) , University of Belgrade , Belgrade , Serbia.
authors.forename: Milos
authors.initials: M
authors.lastname: Nikolic
authors.affiliation: b Mycological Laboratory, Department of Plant Physiology, Institute for Biological Research "Sinisa Stankovic" (IBISS) , University of Belgrade , Belgrade , Serbia.
authors.forename: Marina
authors.initials: M
authors.lastname: Sokovic
mesh_terms: D000872:Anthocyanins / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D011710:Pyocyanine / Q000096:biosynthesis; D053038:Quorum Sensing / Q000187:drug effects*
chemical_list: D000872:Anthocyanins; D000900:Anti-Bacterial Agents; D011710:Pyocyanine; C017185:delphinidin
keywords: Flavanone-7-O-glucoside; anti-biofilm activity; bacterial resistance; delphinidin; infectious diseases; low sub-MIC