title: The predictive value of selected serum microRNAs for acute GVHD by TaqMan MicroRNA arrays.
abstract: Currently, the diagnosis of acute graft-versus-host disease (aGVHD) is mainly based on clinical symptoms and biopsy results. This study was designed to further explore new no noninvasive biomarkers for aGVHD prediction/diagnosis. We profiled miRNAs in serum pools from patients with aGVHD (grades II-IV) (n = 9) and non-aGVHD controls (n = 9) by real-time qPCR-based TaqMan MicroRNA arrays. Then, predictive models were established using related miRNAs (n = 38) and verified by a double-blind trial (n = 54). We found that miR-411 was significantly down regulated when aGVHD developed and recovered when aGVHD was controlled, which demonstrated that miR-411 has potential as an indicator for aGVHD monitoring. We developed and validated a predictive model and a diagnostic model for aGVHD. The predictive model included two miRNAs (miR-26b and miR-374a), which could predict an increased risk for aGVHD 1 or 2 weeks in advance, with an AUC, Positive Predictive Value (PPV), and Negative Predictive Value (NPV) of 0.722, 76.19 %, and 69.70 %, respectively. The diagnostic model included three miRNAs (miR-28-5p, miR-489, and miR-671-3p) with an AUC, PPV, and NPV of 0.841, 85.71 % and 83.33 %, respectively. Our results show that circulating miRNAs (miR-26b and miR-374a, miR-28-5p, miR-489 and miR-671-3p) may serve as biomarkers for the prediction and diagnosis of grades II-IV aGVHD.
authors.affiliation: Core Laboratory of Translational Medicine, State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, 28 Fu-Xing Road, Beijing, 100853, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Genetic and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe E Rd, Zhengzhou, 450052, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Bai
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, 28 Fu-Xing Road, Beijing, 100853, China.
authors.forename: Wenrong
authors.initials: W
authors.lastname: Huang
authors.affiliation: Core Laboratory of Translational Medicine, State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, 28 Fu-Xing Road, Beijing, 100853, China.
authors.forename: Pengjun
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Core Laboratory of Translational Medicine, State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, 28 Fu-Xing Road, Beijing, 100853, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Clinical Biochemistry, Chinese PLA General Hospital, 28 Fu-Xing Road, Beijing, 100853, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Men
authors.affiliation: Department of Clinical Biochemistry, Chinese PLA General Hospital, 28 Fu-Xing Road, Beijing, 100853, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wen
authors.affiliation: Department of Clinical Biochemistry, Chinese PLA General Hospital, 28 Fu-Xing Road, Beijing, 100853, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Tong
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, 28 Fu-Xing Road, Beijing, 100853, China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Wang
authors.affiliation: Core Laboratory of Translational Medicine, State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, 28 Fu-Xing Road, Beijing, 100853, China. Tianyp@301hospital.com.cn.|Department of Clinical Biochemistry, Chinese PLA General Hospital, 28 Fu-Xing Road, Beijing, 100853, China. Tianyp@301hospital.com.cn.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Tian
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D064591:Allografts; D019540:Area Under Curve; D015415:Biomarkers; D004311:Double-Blind Method; D005260:Female; D006086:Graft vs Host Disease / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D008954:Models, Biological; D011237:Predictive Value of Tests; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D016037:Single-Blind Method; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D055815:Young Adult
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha
keywords: Acute graft-versus-host disease; Biomarker; Diagnosis; MicroRNA array; Plasma microRNA; Prediction