title: Development of novel polymorphic microsatellite markers in Siganus fuscescens.
abstract: Rabbitfish, Siganus fuscescens, is widely distributed in the Indo-Pacific regions and eastern Mediterranean. Its dwelling place includes reef flats, coral reef regions, and seagrass meadows in tropical area and reef areas or shallow waters in locations at high latitudes. In the present study, 10 new polymorphic microsatellite markers were screened from 30 wild S. fuscescens individuals, using a method of fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. The number of polymorphic alleles per locus was 3 to 5 with a mean of 4.3, while the value of polymorphic information content ranged from 0.283 to 0.680. The values of the observed and expected heterozygosities were in the range 0.3333-0.8462 and 0.3011-0.7424, respectively. Deviation from Hardy-Weinberg equilibrium was not observed in this study. These polymorphic loci are expected to be effective in evaluating the genetic diversity, population structure, and gene flow and in determining the paternity in S. fuscescens, as well as for conservation management.
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Mao
authors.affiliation: Fisheries College, Jimei University, Xiamen, China lizhongbao@jmu.edu.cn.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China lizhongbao@jmu.edu.cn.
authors.forename: Z B
authors.initials: ZB
authors.lastname: Li
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Ning
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
authors.forename: J B
authors.initials: JB
authors.lastname: Shangguan
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Huang
authors.affiliation: Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
authors.forename: B B
authors.initials: BB
authors.lastname: Li
mesh_terms: D000818:Animals; D003247:Conservation of Natural Resources; D057894:Coral Reefs; D005399:Fishes / Q000235:genetics*; D051456:Gene Flow; D056426:Genetic Loci; D005819:Genetic Markers; D014644:Genetic Variation; D006579:Heterozygote; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers