title: Response of BAX, Bcl-2 Proteins, and SIRT1/PGC-1α mRNA Expression to 8-Week Treadmill Running in the Aging Rat Skeletal Muscle.
abstract: The aim of this study was to analyze the effects of exercise training on Bax and Bcl-2 protein content and sirtuin1 (SIRT1) mRNA expression levels to prevent sarcopenia in aging rats. Eight 18 months old male Sprague-Dawley rats were trained 5 days weekly for 8 weeks on a treadmill, and eight sedentary rats served as controls. Gastrocnemius muscles were dissected 2 days after the last training session. The mRNA content of PGC-1α, caspase-3, NRF1, TFAM, SOD2, and SIRT1 was estimated by RT-PCR with GAPDH used as an internal control. The protein expression of BAX and Bcl-2 was assessed by Western immunoblot. After training, significant (p < 0.05) increases were noted for the gastrocnemius muscle weights, the gastrocnemius mass/body mass ratio, the bcl-2/BAX ratio, the Bcl-2 protein and the SIRT1, PGC-1α, NRF1, TFAM, SOD2 mRNA content in the trained gastrocnemius, relative to the control samples. No difference was found in the BAX protein between control and trained muscles, whereas the caspase-3 mRNA content decreased by 50 %, in the gastrocnemius muscle of trained animals. Exercise training may inhibit age-induced myonuclear apoptosis by stimulating SIRT1/PGC-1α mRNA expression, thereby preventing sarcopenia in aging rat.
authors.affiliation: School of Physical Education and Health, Zhaoqing University, Zhaoqing, 526061, China. fanghuili2007@163.com.
authors.forename: Fang-Hui
authors.initials: FH
authors.lastname: Li
authors.affiliation: School of Physical Education and Health, Zhaoqing University, Zhaoqing, 526061, China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Yu
authors.affiliation: School of Physical Education and Health, Zhaoqing University, Zhaoqing, 526061, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xiao
authors.affiliation: School of Physical Education and Health, Zhaoqing University, Zhaoqing, 526061, China.
authors.forename: Yan-Ying
authors.initials: YY
authors.lastname: Liu
mesh_terms: D000367:Age Factors; D000375:Aging / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000473:pathology; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000235:genetics / Q000378:metabolism*; D005082:Physical Exertion; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012420:Running; D055948:Sarcopenia / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D015398:Signal Transduction; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: C494828:Bax protein, rat; C000617196:Bcl2 protein, rat; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C501429:Ppargc1a protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; C505410:Casp3 protein, rat; D053148:Caspase 3; C488368:Sirt1 protein, rat; D056564:Sirtuin 1
keywords: Aging rat; Exercise training; Mitochondrial; Sarcopenia; Sirtuin1