title: Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures.
abstract: Functional genomics research can give us valuable insights into bacterial gene function. RNA Sequencing (RNA-seq) can generate information on transcript abundance in bacteria following abiotic stress treatments. In this study, we used the RNA-seq technique to study the transcriptomes of the opportunistic nosocomial pathogen Pseudomonas aeruginosa PAO1 following heat shock. Samples were grown at both the human body temperature (37 °C) and an arbitrarily-selected temperature of 46 °C. In this work using RNA-seq, we identified 133 genes that are differentially expressed at 46 °C compared to the human body temperature. Our work identifies some key P. aeruginosa PAO1 genes whose products have importance in both environmental adaptation as well as in vivo infection in febrile hosts. More importantly, our transcriptomic results show that many genes are only expressed when subjected to heat shock. Because the RNA-seq can generate high throughput gene expression profiles, our work reveals many unanticipated genes with further work to be done exploring such genes products.
authors.affiliation: ISB (Genetics & Molecular Biology), Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Kok-Gan
authors.identifier: 0000-0002-1883-1115
authors.initials: KG
authors.lastname: Chan
authors.affiliation: ISB (Genetics & Molecular Biology), Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Kumutha
authors.initials: K
authors.lastname: Priya
authors.affiliation: School of Life Sciences, Heriot-Watt University , Edinburgh , United Kingdom.
authors.forename: Chien-Yi
authors.initials: CY
authors.lastname: Chang
authors.affiliation: BioEasy Sdn Bhd , Shah Alam, Selangor , Malaysia.
authors.forename: Ahmad Yamin
authors.initials: AY
authors.lastname: Abdul Rahman
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Kok Keng
authors.initials: KK
authors.lastname: Tee
authors.affiliation: ISB (Genetics & Molecular Biology), Faculty of Science, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
keywords: Gene expression; Heat shock; Pseudomonas aeruginosa PAO1; RNA sequencing; Transcriptome