title: Label-Free Photonic Crystal-Based β-Lactamase Biosensor for β-Lactam Antibiotic and β-Lactamase Inhibitor.
abstract: A simple, label-free, and visual photonic crystal-based β-lactamase biosensor was developed for β-lactam antibiotic and β-lactamase inhibitor in which the penicillinase (a β-lactamase) was immobilized on the pH-sensitive colloidal crystal hydrogel (CCH) film to form penicillinase colloidal crystal hydrogel (PCCH) biosensing film. The hydrolysis of penicillin G (a β-lactam antibiotic) can be catalyzed by penicillinase to produce penicilloic acid, leading to a pH decrease in the microenvironment of PCCH film, which causes the shrink of pH-sensitive CCH film and triggers a blue-shift of the diffraction wavelength. Upon the addition of β-lactamase inhibitor, the hydrolysis reaction is suppressed and no clear blue-shift is observed. The concentrations of β-lactam antibiotic and β-lactamase inhibitor can be sensitively evaluated by measuring the diffraction shifts. The minimum detectable concentrations for penicillin G and clavulanate potassium (a β-lactamase inhibitor) can reach 1 and 0.1 μM, respectively. Furthermore, the proposed method is highly reversible and selective, and it allows determination of penicillin G in fish pond water samples.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, People's Republic of China.
authors.forename: Fubing
authors.initials: F
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, People's Republic of China.
authors.forename: Guoguo
authors.initials: G
authors.lastname: Li
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, People's Republic of China.
authors.forename: Qianshan
authors.initials: Q
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, People's Republic of China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, People's Republic of China.
authors.forename: Ruqin
authors.initials: R
authors.lastname: Yu
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D001409:Bacillus cereus / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D019818:Clavulanic Acid / Q000032:analysis*; D004800:Enzymes, Immobilized / Q000378:metabolism; D005618:Fresh Water / Q000032:analysis; D057230:Limit of Detection; D010400:Penicillin G / Q000032:analysis*; D010405:Penicillinase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis*; D065093:beta-Lactamase Inhibitors / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D004800:Enzymes, Immobilized; D014874:Water Pollutants, Chemical; D065093:beta-Lactamase Inhibitors; D019818:Clavulanic Acid; D010405:Penicillinase; D010400:Penicillin G