title: A Potential Nanofiber Membrane Device for Filling Surgical Residual Cavity to Prevent Glioma Recurrence and Improve Local Neural Tissue Reconstruction.
abstract: This study aims to develop a novel device with nanofiber membrane capable of sustained release of temozolomide (TMZ) and neuron growth factor (NGF). An improved bio-availability of TMZ and NGF in surroundings proximal to the device was expected to be attained for a prolonged period of time. The device was developed by integrating TMZ-doped polycaprolactone (PCL) nanofiber (TP) membrane and NGF-coated PCL (NGFP) membrane using sodium alginate hydrogel. TP was prepared by direct electrospinning of TMZ/PCL. NGFP membrane was developed by layer-by-layer assembling technology. The incorporation of TMZ-doped nanofiber and NGFP nanofiber in the device was confirmed by scanning electron microscopy. The number of NGF layer in NGF-coated PCL membrane could be readily measured with energy spectrum analysis. The in vitro release study showed that TP-NGFP-TP membrane could efficiently liberate TMZ to inhibit the growth of C6 glioma cells, and sufficient NGF to induce the differentiation of PC12 neuron cells over four weeks. Such TP-NGFP-TP membrane device can be employed as a tampon to fill up surgical residual cavity and afford residual glioma removal, structural support, hemostasis, and local neural tissue reconstruction in the surgical treatment of glioma. The study opens a horizon to develop multifunctional biomaterial device for maximized glioma treatment efficacy.
authors.affiliation: The Institute for Translational Nanomedicine, Shanghai East Hospital, Institute of Biomedical Engineering and Nanoscience, Tongji University School of Medicine, Shanghai, People's Republic of China.|Institute of Nano and Bio-Polymeric Materials, School of Material Science and Engineering, Tongji University, Shanghai, People's Republic of China.
authors.forename: Daoxiang
authors.initials: D
authors.lastname: Huang
authors.affiliation: The Institute for Translational Nanomedicine, Shanghai East Hospital, Institute of Biomedical Engineering and Nanoscience, Tongji University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lin
authors.affiliation: The Institute for Translational Nanomedicine, Shanghai East Hospital, Institute of Biomedical Engineering and Nanoscience, Tongji University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Wen
authors.affiliation: Key Laboratory of Advanced Civil Engineering Materials Ministry of Education, School of Material Science and Engineering, Tongji University, Shanghai, People's Republic of China.|Institute of Nano and Bio-Polymeric Materials, School of Material Science and Engineering, Tongji University, Shanghai, People's Republic of China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Gu
authors.affiliation: The Institute for Translational Nanomedicine, Shanghai East Hospital, Institute of Biomedical Engineering and Nanoscience, Tongji University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000737:chemistry / Q000494:pharmacology*; D001682:Biological Availability; D045744:Cell Line, Tumor; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000138:chemical synthesis*; D004337:Drug Carriers; D004339:Drug Compounding; D065546:Drug Liberation; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D020932:Nerve Growth Factor / Q000737:chemistry / Q000494:pharmacology*; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011091:Polyesters / Q000737:chemistry; D051381:Rats; D000077204:Temozolomide
chemical_list: D000464:Alginates; D018906:Antineoplastic Agents, Alkylating; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006603:Hexuronic Acids; D020100:Hydrogels; D011091:Polyesters; C016240:polycaprolactone; D003606:Dacarbazine; D020723:Glucuronic Acid; D020932:Nerve Growth Factor; D000077204:Temozolomide