title: Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time.
abstract: Live cell imaging can provide important information on cellular dynamics; however, the full utilisation of this technology has been hampered by the limitations of imaging reagents. Metal-based complexes have the potential to overcome many of the issues common to many current imaging agents. The rhenium (I)-based complex fac-[Re(CO)3 (1,10-phenanthroline)(4-pyridyltetrazolate)], herein referred to as ReZolve-ER(™) , shows promise as a live cell imaging agent with rapid cell uptake, low cytotoxicity, resistance to photobleaching and compatibility with multicolour imaging. ReZolve-ER(™) localised to the nuclear membrane/endoplasmic reticulum (ER) and allowed the detection of exocytotic events at the plasma membrane. Thus, we present a new imaging agent for monitoring live cell events in real time, which is ideal for imaging either short- or long-time courses.
authors.affiliation: Mechanisms in Cell Biology and Disease Research Group, School of Pharmacy and Medical Sciences, Sansom Institute for Health Research, University of South Australia, Adelaide, Australia.
authors.forename: Christie A
authors.initials: CA
authors.lastname: Bader
authors.affiliation: Mechanisms in Cell Biology and Disease Research Group, School of Pharmacy and Medical Sciences, Sansom Institute for Health Research, University of South Australia, Adelaide, Australia.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Sorvina
authors.affiliation: Department of Chemistry and Nanochemistry Research Institute, Curtin University, Bently, Australia.
authors.forename: Peter V
authors.initials: PV
authors.lastname: Simpson
authors.affiliation: Department of Chemistry and Nanochemistry Research Institute, Curtin University, Bently, Australia.
authors.forename: Phillip J
authors.initials: PJ
authors.lastname: Wright
authors.affiliation: Department of Industrial Chemistry 'Toso Montanari', University of Bologna, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Stagni
authors.affiliation: Mechanisms in Cell Biology and Disease Research Group, School of Pharmacy and Medical Sciences, Sansom Institute for Health Research, University of South Australia, Adelaide, Australia.
authors.forename: Sally E
authors.initials: SE
authors.lastname: Plush
authors.affiliation: Department of Chemistry and Nanochemistry Research Institute, Curtin University, Bently, Australia.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Massi
authors.affiliation: Mechanisms in Cell Biology and Disease Research Group, School of Pharmacy and Medical Sciences, Sansom Institute for Health Research, University of South Australia, Adelaide, Australia. doug.brooks@unisa.edu.au.
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Brooks
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005089:Exocytosis; D005456:Fluorescent Dyes; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D009942:Organometallic Compounds; D010618:Phenanthrolines; D012211:Rhenium
chemical_list: D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D010618:Phenanthrolines; D012211:Rhenium
keywords: endoplasmic reticulum; live cell imaging; rhenium