title: Characterization and Selection of 3-(1-Naphthoyl)-Indole Derivative-Specific Alpaca VHH Antibodies Using a Phage Display Library.
abstract: A new alpaca VHH antibody library against 3-(1-naphthoyl)-indole derivatives was developed from alpaca immunized with 7-(3-(1-naphthoyl)-1H-indol-1-yl)-heptanoic acid-keyhole limpet hemocyanin (Hep-KLH) protein conjugates as the immunogen. From this library, two 3-(1-naphthoyl)-indole derivative-specific clones, named NN01 and NN02, were isolated using biopanning technology. The binding specificity of these clones was confirmed using a competitive enzyme-linked immunosorbent assay (c-ELISA). Based on the results of c-ELISA, a median inhibitory concentration (IC50) of these two VHH antibodies, NN01 and NN02, in the case of 7-(3-(1-naphthoyl)-1H-indol-1-yl)-heptanoic acid (Hep; one of 3-(1-naphthoyl)-indole derivatives) as an inhibitor exhibited an approximate 3 × 10(-7) M and 6 × 10(-7) M, respectively. Thus, VHH antibodies produced in this study could be considered a useful tool for the detection of 3-(1-naphthoyl)-indole derivatives.
authors.affiliation: 1 Device Research Laboratory, Advanced Research Division, Panasonic Co., Ltd. , Soraku-gun, Japan .|2 Graduate School of Science and Engineering, Kagoshima University , Soraku-gun, Japan .
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakayama
authors.affiliation: 3 Graduate School of Medicine, University of the Ryukyus , Nishihara, Japan .
authors.forename: Akikazu
authors.initials: A
authors.lastname: Murakami
authors.affiliation: 4 Antibody Engineering Research Center, Inc. , Noda, Japan .
authors.forename: Maiko
authors.initials: M
authors.lastname: Yoshida
authors.affiliation: 1 Device Research Laboratory, Advanced Research Division, Panasonic Co., Ltd. , Soraku-gun, Japan .
authors.forename: Jin
authors.initials: J
authors.lastname: Muraoka
authors.affiliation: 1 Device Research Laboratory, Advanced Research Division, Panasonic Co., Ltd. , Soraku-gun, Japan .
authors.forename: Junko
authors.initials: J
authors.lastname: Wakai
authors.affiliation: 1 Device Research Laboratory, Advanced Research Division, Panasonic Co., Ltd. , Soraku-gun, Japan .
authors.forename: Noriko
authors.initials: N
authors.lastname: Kenjyou
authors.affiliation: 2 Graduate School of Science and Engineering, Kagoshima University , Soraku-gun, Japan .
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D000941:Antigens / Q000276:immunology; D002161:Camelids, New World / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007211:Indoles / Q000276:immunology; D019151:Peptide Library; D061905:Single-Domain Antibodies / Q000096:biosynthesis / Q000276:immunology*
chemical_list: D000906:Antibodies; D000941:Antigens; D007211:Indoles; D019151:Peptide Library; D061905:Single-Domain Antibodies; C030374:indole