title: [Qualitative analysis of bis-(3'-5')-cyclic dimeric adenosine monophosphate of Porphyromonas gingivalis by high performance liquid chromatography coupled with mass spectrometry].
abstract: OBJECTIVE
  To test whether Porphyromonas gingivalis (P. gingivalis) could produce bacterial signal molecule, bis-(3'-5')-cyclic dimeric adenosine monophosphate (c-di-AMP) and lay the foundation for explorations of its roles in life metabolism and periodontitis immunity of P. gingivalis.
  
  
  METHODS
  P. gingivalis standard strain ATCC33277 was used as the experimental strain to extract nucleic acids from the bacteria. Then, c-di-AMP was detected using high performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS). Subsequently, HPLC was used to validate the sample further.
  
  
  RESULTS
  Based on the signal/noise (S/N) for 3 : 1, the limit of determination of HPLC-MS/MS for peak time of c-di-AMP standard substances was 7.49 min and nucleic acid extractions from P. gingivalis was 8.82 min (S/N > 3). Further confirmation of HPLC showed that nucleic acid extractions from both P. gingivalis and c-di-AMP standard substances pre- sented goal absorbent peaks at 15.7 min, with the same ultraviolet absorbent spectrum.
  
  
  CONCLUSIONS
  The nucleic acid extrac- tions from P. gingivalis contained c-di-AMP, which shows that P. gingivalis could produce c-di-AMP.
authors.forename: Tan
authors.initials: T
authors.lastname: Yongmei
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiaojun
authors.forename: Du
authors.initials: D
authors.lastname: Juan
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wanghong
authors.forename: Chen
authors.initials: C
authors.lastname: Xiaodan
authors.forename: Hou
authors.initials: H
authors.lastname: Jin
mesh_terms: D002851:Chromatography, High Pressure Liquid; D000242:Cyclic AMP / Q000737:chemistry / Q000378:metabolism*; D010518:Periodontitis; D016966:Porphyromonas gingivalis / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D000242:Cyclic AMP