title: Assessment of Sugar Components and Genes Involved in the Regulation of Sucrose Accumulation in Peach Fruit.
abstract: Soluble sugar contents in mature fruits of 45 peach accessions were quantified using gas chromatography analysis. Sucrose is the predominant sugar in mature fruit, followed by glucose and fructose, which have similar concentrations. Overall, sucrose metabolism and accumulation are crucial determinants of sugar content in peach fruit, and there is a wide range of sucrose concentrations among peach genotypes. To understand the mechanisms regulating sucrose accumulation in peach fruit, expression profiles of genes involved in sucrose metabolism and transport were compared among four genotypes. Two sucrose-cleaving enzyme genes (SUS4 and NINV8), one gene involved in sucrose resynthesis (SPS3), and three sugar transporter genes (SUT2, SUT4, and TMT2) were prevalently expressed in peach fruit, and their expression levels are significantly correlated with sucrose accumulation. In contrast, the VAINV genes responsible for sucrose cleavage in the vacuole were weakly expressed in mature fruit, suggesting that the sucrose-cleaving reaction is not active in the vacuole of sink cells of mature peach fruit. This study suggests that sucrose accumulation in peach fruit involves the coordinated interaction of genes related to sucrose cleavage, resynthesis, and transport, which could be helpful for future peach breeding.
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of the Chinese Academy of Sciences , Wuhan 430074, People's Republic of China.|Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University , Bangkok 10330, Thailand.
authors.forename: Sornkanok
authors.initials: S
authors.lastname: Vimolmangkang
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of the Chinese Academy of Sciences , Wuhan 430074, People's Republic of China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of the Chinese Academy of Sciences , Wuhan 430074, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Peng
authors.affiliation: Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences , A12, Ruiwangfen, Beijing 100093, People's Republic of China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Institute of Fruit Tree and Tea, Hubei Academy of Agricultural Sciences , 430209 Wuhan, People's Republic of China.
authors.forename: Huiliang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of the Chinese Academy of Sciences , Wuhan 430074, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Fang
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of the Chinese Academy of Sciences , Wuhan 430074, People's Republic of China.|Sino-African Joint Research Center, Chinese Academy of Sciences , Wuhan 430074, People's Republic of China.
authors.forename: Liao
authors.initials: L
authors.lastname: Liao
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of the Chinese Academy of Sciences , Wuhan 430074, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Fruit Tree and Tea, Hubei Academy of Agricultural Sciences , 430209 Wuhan, People's Republic of China.
authors.forename: Huaping
authors.initials: H
authors.lastname: He
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of the Chinese Academy of Sciences , Wuhan 430074, People's Republic of China.|Sino-African Joint Research Center, Chinese Academy of Sciences , Wuhan 430074, People's Republic of China.
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Han
mesh_terms: D005421:Flavoring Agents / Q000032:analysis / Q000378:metabolism; D005632:Fructose / Q000032:analysis / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005838:Genotype; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D000068239:Prunus persica / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013395:Sucrose / Q000032:analysis / Q000378:metabolism*
chemical_list: D005421:Flavoring Agents; D010940:Plant Proteins; D005632:Fructose; D013395:Sucrose; D005947:Glucose
keywords: Prunus persica; soluble sugar; sucrose metabolic genes; sugar transporter genes; sweetness