title: Rapid Identification of OXA-48 and OXA-163 Subfamilies in Carbapenem-Resistant Gram-Negative Bacilli with a Novel Immunochromatographic Lateral Flow Assay.
abstract: We assessed a novel immunochromatographic lateral flow assay for direct identification of OXA-48-like carbapenemases and accurate differentiation of allele variants with distinct substrate profiles (OXA-48 or OXA-163 subfamilies). The assay allowed rapid (less than 4 min) and reliable direct confirmation of OXA-163- and/or OXA-48-like enzymes (with 100% sensitivity and 100% specificity) from cultured colonies that were recovered from both solid medium and spiked blood culture bottles.
authors.affiliation: Servicio Antimicrobianos, Laboratorio Nacional y Regional de Referencia en Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas (INEI), ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud de la Nación, Ciudad Autónoma de Buenos Aires, Argentina fpasteran@gmail.com.
authors.forename: Fernando
authors.initials: F
authors.lastname: Pasteran
authors.affiliation: Coris BioConcept, Gembloux, Belgium.
authors.forename: Laurence
authors.initials: L
authors.lastname: Denorme
authors.affiliation: Coris BioConcept, Gembloux, Belgium.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Ote
authors.affiliation: Servicio Antimicrobianos, Laboratorio Nacional y Regional de Referencia en Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas (INEI), ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud de la Nación, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Sonia
authors.initials: S
authors.lastname: Gomez
authors.affiliation: Servicio Antimicrobianos, Laboratorio Nacional y Regional de Referencia en Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas (INEI), ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud de la Nación, Ciudad Autónoma de Buenos Aires, Argentina.|Laboratorio de Resistencia Bacteriana, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Denise
authors.initials: D
authors.lastname: De Belder
authors.affiliation: National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne, UCL Namur, Yvoir, Belgium.
authors.forename: Youri
authors.initials: Y
authors.lastname: Glupczynski
authors.affiliation: National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne, UCL Namur, Yvoir, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Bogaerts
authors.affiliation: Laboratorio de Resistencia Bacteriana, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Barbara
authors.initials: B
authors.lastname: Ghiglione
authors.affiliation: Laboratorio de Resistencia Bacteriana, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Pablo
authors.initials: P
authors.lastname: Power
authors.affiliation: Coris BioConcept, Gembloux, Belgium.
authors.forename: Pascal
authors.initials: P
authors.lastname: Mertens
authors.affiliation: Servicio Antimicrobianos, Laboratorio Nacional y Regional de Referencia en Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas (INEI), ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud de la Nación, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Corso
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000201:enzymology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001618:beta-Lactamases