title: Effects of isoxsuprine hydrochloride on electrocardiographic and trace element status in sheeps.
abstract: OBJECTIVE
  To study the effect of isoxsuprine hydrochloride on the ischaemic electrocardiographic change and trace element status in sheep.
  
  
  METHODS
  This study was conducted from March 16 to 23, 2012, at Istanbul University, Turkey, and comprised sheep aged 6 months. The animals were divided into two equal groups. The control group was fed a standard diet and had free access to water. In the experimental group, isoxsuprine hydrochloride was injected at a dose of 0.6 mg/kg through the intramuscular route. Electrocardiographic changes, including creatine kinase and cardiac troponin-I, and serum levels of selenium, copper, calcium, magnesium, iron and zinc were investigated in healthy sheep. SPSS 15 was used for statistical analysis.
  
  
  RESULTS
  The 14 sheep were divided into two groups of 7(50%) each. The overall mean weight of the study population was 35±10kg. Selenium, calcium, iron and zinc concentrations did not show any difference in serum samples (p>0.05). However, copper and magnesium concentrations decreased in serum after the administration of the drug (p<0.05). In the experimental group, ST segment depression and abnormal T-wave was found in 6(86%) animals within 60min.
  
  
  CONCLUSIONS
  Isoxsuprine hydrochloride increased cardiotoxicity risk in sheep.
authors.affiliation: Department of Pharmacology and Toxicology, Istanbul, Turkey.
authors.forename: Handan Aydin
authors.initials: HA
authors.lastname: Vural
authors.affiliation: Division of Hematology, Department of Internal Medicine, Bezmialem Vakif University Medical Faculty, Istanbul, Turkey.
authors.forename: Lora
authors.initials: L
authors.lastname: Koenhemsi
authors.affiliation: Department of Internal Medicine, Istanbul, Turkey.
authors.forename: Remzi
authors.initials: R
authors.lastname: Gonul
authors.affiliation: Department of Biochemistry, Istanbul University, Faculty of Veterinary Medicine, Istanbul, Turkey.
authors.forename: Hasret Demircan
authors.initials: HD
authors.lastname: Yardibi
authors.affiliation: Department of Biophysic, Trakya University, Medical School, Edirne, Istanbul, Turkey.
authors.forename: Tevfik
authors.initials: T
authors.lastname: Gulyasar
authors.affiliation: Department of Internal Medicine, Istanbul, Turkey.
authors.forename: Erman
authors.initials: E
authors.lastname: Or
authors.affiliation: Department of Biochemistry, Istanbul University, Faculty of Veterinary Medicine, Istanbu.
authors.forename: Gulhan Turkay
authors.initials: GT
authors.lastname: Hosturk
authors.affiliation: Department of Biophysic.
authors.forename: Bora
authors.initials: B
authors.lastname: Barutcu
authors.affiliation: Fikret Biyal Central Research Laboratory, Istanbul University,Cerrahpasa MedicalSchool, Istanbul,Turkey.
authors.forename: Huriye
authors.initials: H
authors.lastname: Balci
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000097:blood; D003300:Copper / Q000097:blood; D052279:Creatine Kinase, MB Form / Q000097:blood / Q000187:drug effects*; D004562:Electrocardiography; D006321:Heart / Q000187:drug effects*; D007501:Iron / Q000097:blood; D007556:Isoxsuprine / Q000494:pharmacology*; D008274:Magnesium / Q000097:blood; D012643:Selenium / Q000097:blood; D012756:Sheep; D013739:Testosterone / Q000097:blood; D019210:Troponin I / Q000097:blood / Q000187:drug effects*; D015032:Zinc / Q000097:blood
chemical_list: D000318:Adrenergic beta-Agonists; D019210:Troponin I; D013739:Testosterone; D003300:Copper; D007501:Iron; D052279:Creatine Kinase, MB Form; D012643:Selenium; D008274:Magnesium; D015032:Zinc; D007556:Isoxsuprine; D002118:Calcium
keywords: Isoxsuprine hydrochloride, Sheep, Cardiac troponin-I, cTnI, Creatine kinase-MB, CK-MB.