title: 1α,25(OH)₂D₃ Suppresses the Migration of Ovarian Cancer SKOV-3 Cells through the Inhibition of Epithelial-Mesenchymal Transition.
abstract: Ovarian cancer is the most lethal gynecological malignancy due to its high metastatic ability. Epithelial-mesenchymal transition (EMT) is essential during both follicular rupture and epithelium regeneration. However, it may also accelerate the progression of ovarian carcinomas. Experimental studies have found that 1α,25-dihydroxyvitamin-D3 [1α,25(OH)₂D₃] can inhibit the proliferation of ovarian cancer cells. In this study, we investigated whether 1α,25(OH)₂D₃ could inhibit the migration of ovarian cancer cells via regulating EMT. We established a model of transient transforming growth factor-β1(TGF-β1)-induced EMT in human ovarian adenocarcinoma cell line SKOV-3 cells. Results showed that, compared with control, 1α,25(OH)₂D₃ not only inhibited the migration and the invasion of SKOV-3 cells, but also promoted the acquisition of an epithelial phenotype of SKOV-3 cells treated with TGF-β1. We discovered that 1α,25(OH)₂D₃ increased the expression of epithelial marker E-cadherin and decreased the level of mesenchymal marker, Vimentin, which was associated with the elevated expression of VDR. Moreover, 1α,25(OH)₂D₃ reduced the expression level of transcription factors of EMT, such as slug, snail, and β-catenin. These results indicate that 1α,25(OH)₂D₃ suppresses the migration and invasion of ovarian cancer cells by inhibiting EMT, implying that 1α,25(OH)₂D₃ might be a potential therapeutic agent for the treatment of ovarian cancer.
authors.affiliation: Department of Toxicology, School of Public Health, Soochow University, Suzhou 215123, China. 20144247024@stu.suda.edu.cn.
authors.forename: Yong-Feng
authors.initials: YF
authors.lastname: Hou
authors.affiliation: Department of Nutrition and Food Hygiene, Wenzhou Center for Disease Control and Prevention, Wenzhou 325000, China. gaosihai1987@163.com.
authors.forename: Si-Hai
authors.initials: SH
authors.lastname: Gao
authors.affiliation: Organ Transplant Institute, Fuzhou General Hospital, Fuzhou 350025, China. pingwangsuda@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: AIDS-STDs Prevention and Control Department, Wenzhou Center for Disease Control and Prevention, Wenzhou 325000, China. zhanghemei0306@126.com.
authors.forename: He-Mei
authors.initials: HM
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China. zhililiu2@sina.com.
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Liu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou 215123, China. 20154247021@stu.suda.edu.cn.
authors.forename: Meng-Xuan
authors.initials: MX
authors.lastname: Ye
authors.affiliation: Department of Radiation Biology, School of Radiation Medication and Protection, Soochow University, Suzhou 215123, China. gmzhou@suda.edu.cn.
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Zhou
authors.affiliation: Department of Labor Hygiene and Environmental Health, School of Public Health, Soochow University, Suzhou 215123, China. zhangzengli@suda.edu.cn.
authors.forename: Zeng-Li
authors.initials: ZL
authors.lastname: Zhang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou 215123, China. bingyanli@suda.edu.cn.
authors.forename: Bing-Yan
authors.initials: BY
authors.lastname: Li
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002762:Cholecalciferol / Q000494:pharmacology*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D014746:Vimentin / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015820:Cadherins; D053773:Transforming Growth Factor beta1; D014746:Vimentin; D051176:beta Catenin; D002762:Cholecalciferol
keywords: EMT; migration; ovarian cancer; vitamin D