title: Leishmania infantum and Leishmania braziliensis: Differences and Similarities to Evade the Innate Immune System.
abstract: Visceral leishmaniasis is a severe form of the disease, caused by Leishmania infantum in the New World. Patients present an anergic immune response that favors parasite establishment and spreading through tissues like bone marrow and liver. On the other hand, Leishmania braziliensis causes localized cutaneous lesions, which can be self-healing in some individuals. Interactions between host and parasite are essential to understand disease pathogenesis and progression. In this context, dendritic cells (DCs) act as essential bridges that connect innate and adaptive immune responses. In this way, the aim of this study was to compare the effects of these two Leishmania species, in some aspects of human DCs' biology for better understanding of the evasion mechanisms of Leishmania from host innate immune response. To do so, DCs were obtained from monocytes from whole peripheral blood of healthy volunteer donors and from those infected with L. infantum or L. braziliensis for 24 h. We observed similar rates of infection (around 40%) as well as parasite burden for both Leishmania species. Concerning surface molecules, we observed that both parasites induced CD86 expression when DCs were infected for 24 h. On the other hand, we detected a lower surface expression of CD209 in the presence of both L. braziliensis and L. infantum, but only the last one promoted the survival of DCs after 24 h. Therefore, DCs infected by both Leishmania species showed a higher expression of CD86 and a decrease of CD209 expression, suggesting that both enter DCs through CD209 molecule. However, only L. infantum had the ability to inhibit DC apoptotic death, as an evasion mechanism that enables its spreading to organs like bone marrow and liver. Lastly, L. braziliensis was more silent parasite, once it did not inhibit DC apoptosis in our in vitro model.
authors.affiliation: Department of Cell Biology, Biology Institute, University of Brasília, Brasília, Brazil; Núcleo de Medicina Tropical (NMT), University of Brasília, Brasília, Brazil.
authors.forename: Sarah de Athayde Couto
authors.initials: Sde A
authors.lastname: Falcão
authors.affiliation: Department of Cell Biology, Biology Institute, University of Brasília , Brasília , Brazil.
authors.forename: Tatiana M G
authors.initials: TM
authors.lastname: Jaramillo
authors.affiliation: Núcleo de Medicina Tropical (NMT), University of Brasília , Brasília , Brazil.
authors.forename: Luciana G
authors.initials: LG
authors.lastname: Ferreira
authors.affiliation: Department of Cell Biology, Biology Institute, University of Brasília , Brasília , Brazil.
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Bernardes
authors.affiliation: Department of Cell Biology, Biology Institute, University of Brasília , Brasília , Brazil.
authors.forename: Jaime M
authors.initials: JM
authors.lastname: Santana
authors.affiliation: Department of Cell Biology, Biology Institute, University of Brasília, Brasília, Brazil; Núcleo de Medicina Tropical (NMT), University of Brasília, Brasília, Brazil.
authors.forename: Cecília B F
authors.initials: CB
authors.lastname: Favali
keywords: CD209; Leishmania braziliensis; Leishmania infantum; apoptosis; dendritic cells