title: Metabolic Effects of Chronic T3Administration in the Hypothalamic Paraventricular and Ventromedial Nucleus in Male Rats.
abstract: Thyroid hormone is a key regulator of energy metabolism. Apart from its direct effects on peripheral metabolism, thyroid hormone exerts acute metabolic effects via distinct nuclei within the hypothalamus. Recently we developed a method for chronic and local intrahypothalamic T3administration in rats. The present study evaluated the metabolic effects of T3delivered during either 7 or 28 days to the paraventricular or ventromedial nucleus of the hypothalamus (PVN or VMH). T3administration for 7 days in the PVN decreased only plasma T3. There were no effects on body weight, food intake, plasma glucose concentrations, energy expenditure, locomotor activity, and respiratory exchange rate. In the liver and brown adipose tissue (BAT), there were no changes in mRNA expression of genes involved in glucose metabolism and thermogenesis. T3administration for 7 days in the VMH did not change any of these parameters. T3administration for 28 days in the PVN decreased food intake without affecting body weight, glucose concentrations, and body temperature. Liver and BAT gene expression was unaltered, except for decreased liver Dio1 mRNA. T3administration for 28 days in the VMH did not affect liver and BAT mRNA expression, body weight, food intake, and body temperature, whereas blood glucose concentrations were slightly lower. In conclusion, we showed that chronic T3administration to the PVN or VMH does not affect energy metabolism in a major way. Our results imply that the effects of intrahypothalamic T3administration on metabolism largely depend on the duration of treatment.
authors.affiliation: Department of Endocrinology and Metabolism (Z.Z., E.Fo., Y.S., P.H.B., A.K., E.Fl., A.B.), Academic Medical Center (AMC), University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; and Hypothalamic Integration Mechanisms (A.K.), Netherlands Institute for Neuroscience, 1105 BA Amsterdam, The Netherlands.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology and Metabolism (Z.Z., E.Fo., Y.S., P.H.B., A.K., E.Fl., A.B.), Academic Medical Center (AMC), University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; and Hypothalamic Integration Mechanisms (A.K.), Netherlands Institute for Neuroscience, 1105 BA Amsterdam, The Netherlands.
authors.forename: Ewout
authors.initials: E
authors.lastname: Foppen
authors.affiliation: Department of Endocrinology and Metabolism (Z.Z., E.Fo., Y.S., P.H.B., A.K., E.Fl., A.B.), Academic Medical Center (AMC), University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; and Hypothalamic Integration Mechanisms (A.K.), Netherlands Institute for Neuroscience, 1105 BA Amsterdam, The Netherlands.
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Endocrinology and Metabolism (Z.Z., E.Fo., Y.S., P.H.B., A.K., E.Fl., A.B.), Academic Medical Center (AMC), University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; and Hypothalamic Integration Mechanisms (A.K.), Netherlands Institute for Neuroscience, 1105 BA Amsterdam, The Netherlands.
authors.forename: Peter H
authors.initials: PH
authors.lastname: Bisschop
authors.affiliation: Department of Endocrinology and Metabolism (Z.Z., E.Fo., Y.S., P.H.B., A.K., E.Fl., A.B.), Academic Medical Center (AMC), University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; and Hypothalamic Integration Mechanisms (A.K.), Netherlands Institute for Neuroscience, 1105 BA Amsterdam, The Netherlands.
authors.forename: Andries
authors.initials: A
authors.lastname: Kalsbeek
authors.affiliation: Department of Endocrinology and Metabolism (Z.Z., E.Fo., Y.S., P.H.B., A.K., E.Fl., A.B.), Academic Medical Center (AMC), University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; and Hypothalamic Integration Mechanisms (A.K.), Netherlands Institute for Neuroscience, 1105 BA Amsterdam, The Netherlands.
authors.forename: Eric
authors.initials: E
authors.lastname: Fliers
authors.affiliation: Department of Endocrinology and Metabolism (Z.Z., E.Fo., Y.S., P.H.B., A.K., E.Fl., A.B.), Academic Medical Center (AMC), University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; and Hypothalamic Integration Mechanisms (A.K.), Netherlands Institute for Neuroscience, 1105 BA Amsterdam, The Netherlands.
authors.forename: Anita
authors.initials: A
authors.lastname: Boelen
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose; D001835:Body Weight; D004435:Eating; D004734:Energy Metabolism; D015870:Gene Expression; D008099:Liver / Q000378:metabolism; D008124:Locomotion; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000502:physiology*; D017208:Rats, Wistar; D014284:Triiodothyronine / Q000502:physiology*; D014697:Ventromedial Hypothalamic Nucleus / Q000502:physiology*
chemical_list: D001786:Blood Glucose; D014284:Triiodothyronine