title: Computational analysis of Ebolavirus data: prospects, promises and challenges.
abstract: The ongoing Ebola virus (also known as Zaire ebolavirus, a member of the Ebolavirus family) outbreak in West Africa has so far resulted in >28000 confirmed cases compared with previous Ebolavirus outbreaks that affected a maximum of a few hundred individuals. Hence, Ebolaviruses impose a much greater threat than we may have expected (or hoped). An improved understanding of the virus biology is essential to develop therapeutic and preventive measures and to be better prepared for future outbreaks by members of the Ebolavirus family. Computational investigations can complement wet laboratory research for biosafety level 4 pathogens such as Ebolaviruses for which the wet experimental capacities are limited due to a small number of appropriate containment laboratories. During the current West Africa outbreak, sequence data from many Ebola virus genomes became available providing a rich resource for computational analysis. Here, we consider the studies that have already reported on the computational analysis of these data. A range of properties have been investigated including Ebolavirus evolution and pathogenicity, prediction of micro RNAs and identification of Ebolavirus specific signatures. However, the accuracy of the results remains to be confirmed by wet laboratory experiments. Therefore, communication and exchange between computational and wet laboratory researchers is necessary to make maximum use of computational analyses and to iteratively improve these approaches.
authors.affiliation: School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NH, U.K. m.michaelis@kent.ac.uk.
authors.forename: Martin
authors.initials: M
authors.lastname: Michaelis
authors.affiliation: School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NH, U.K.
authors.forename: Jeremy S
authors.initials: JS
authors.lastname: Rossman
authors.affiliation: School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NH, U.K. m.n.wass@kent.ac.uk
authors.forename: Mark N
authors.initials: MN
authors.lastname: Wass
mesh_terms: D000354:Africa, Western / Q000453:epidemiology; D019295:Computational Biology / Q000379:methods*; D004196:Disease Outbreaks / Q000517:prevention & control*; D029043:Ebolavirus / Q000145:classification / Q000235:genetics / Q000502:physiology*; D019143:Evolution, Molecular; D016679:Genome, Viral / Q000235:genetics; D023281:Genomics / Q000379:methods; D019142:Hemorrhagic Fever, Ebola / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D014764:Viral Proteins / Q000235:genetics
chemical_list: C446938:VP24 protein, Ebola virus; D014764:Viral Proteins
keywords: Ebola; pathogenicity; structural bioinformatics