title: A potential molecular model for studying apoptosis enhanced by the interaction of BCL-G with JAB1 in swine.
abstract: BCL-G, an apoptotic factor in Bcl-2 family, is involved in several kinds of diseases by interacting with several proteins. Although many studies on mouse and human BCL-G have been reported, porcine BCL-G (pBCL-G) has been little investigated. In this study, our results showed that pBCL-G was universally expressed in porcine tissues. The BH2 domain affected the subcellular distribution of pBCL-G protein. pBCL-G could interact with porcine JAB1 (pJAB1), by which its subcellular distribution was affected. pBCL-G promoted staurosporine-induced apoptosis that was significantly enhanced by interaction of pBCL-G with pJAB1. The apoptosis at least partially depended on the activated caspase-8, -9 and -3. Owing to the close phylogenetic distance between pigs and humans and their many physiological similarities, our findings may provide a potential molecular model to study human BCL-G and also may have implications in the treatment of diseases relevant with BCL-G.
authors.affiliation: Department of Microbiology & Immunology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Jiang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Xingye
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Zhanzhan
authors.initials: Z
authors.lastname: Kang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Jingna
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Deli
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001343:Autophagy; D000075686:COP9 Signalosome Complex / Q000378:metabolism*; D002460:Cell Line; D042783:Endothelial Cells / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008958:Models, Molecular; D010447:Peptide Hydrolases / Q000378:metabolism*; D010802:Phylogeny; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D000072417:Protein Domains; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D019311:Staurosporine / Q000737:chemistry; D013552:Swine; D014018:Tissue Distribution
chemical_list: C428448:BCL2L14 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D010447:Peptide Hydrolases; C501488:COPS5 protein, human; D000075686:COP9 Signalosome Complex; D019311:Staurosporine
keywords: Autophagy; enhancement to apoptosis; interaction with porcine JAB1; porcine BCL-G; spatial expression; subcellular localization