title: Fine mapping of the Rsv1-h gene in the soybean cultivar Suweon 97 that confers resistance to two Chinese strains of the soybean mosaic virus.
abstract: The Rsv1 - h gene in cultivar Suweon 97, which confers resistance to SMVs, was mapped to a 97.5-kb location (29,815,195-29,912,667 bp on chromosome 13) in the Rsv1 locus, thereby providing additional insights into the molecular nature underlying variations in resistance alleles in this particular locus. Soybean mosaic virus (SMV) is a well-known devastating pathogen of soybean (Glycine max (L.) Merrill.) causing significant yield losses and seed quality deterioration. A single dominant allele, Rsv1-h, which confers resistance to multiple SMV strains, was previously reported in the cultivar Suweon 97, but its exact location is unknown. In the present study, Suweon 97 was crossed with a SMV-sensitive cultivar, Williams 82. Inoculating 267 F2individuals with two Chinese SMV strains (SC6-N and SC7-N) demonstrated that one single dominant gene confers SMV resistance. Another 1,150 F2individuals were then screened for two simple sequence repeat (SSR) markers (BARCSOYSSR_13_1103 and BARCSOYSSR_13_1187) that flank the Rsv1 locus. Seventy-four recombinants were identified and 20 additional polymorphic SSR markers within the Rsv1 region were then employed in genotyping these recombinants. F2:3and F3:4recombinant lines were also inoculated with SC6-N and SC7-N to determine their phenotypes. The final data revealed that in Suweon 97, the Rsv1-h gene that confers resistance to SC6-N and SC7-N was flanked by BARCSOYSSR_13_1114 and BARCSOYSSR_13_1115, two markers that delimit a 97.5-kb region in the reference Williams 82 genome. In such region, eight genes were present, of which two, Glyma13g184800 and Glyma13g184900, encode the characteristic CC-NBS-LRR type of resistance gene and were considered potential candidates for Rsv1-h.
authors.affiliation: Laboratory of Plant Genetics and Molecular Evolution, School of Life Sciences, Nanjing University, 163 XianLin Avenue, Nanjing, 210023, China.
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Ma
authors.affiliation: Laboratory of Plant Genetics and Molecular Evolution, School of Life Sciences, Nanjing University, 163 XianLin Avenue, Nanjing, 210023, China.
authors.forename: Xiao-Yi
authors.initials: XY
authors.lastname: Wu
authors.affiliation: Laboratory of Plant Genetics and Molecular Evolution, School of Life Sciences, Nanjing University, 163 XianLin Avenue, Nanjing, 210023, China.
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Chen
authors.affiliation: Laboratory of Plant Genetics and Molecular Evolution, School of Life Sciences, Nanjing University, 163 XianLin Avenue, Nanjing, 210023, China.
authors.forename: Ying-Na
authors.initials: YN
authors.lastname: Liu
authors.affiliation: Laboratory of Plant Genetics and Molecular Evolution, School of Life Sciences, Nanjing University, 163 XianLin Avenue, Nanjing, 210023, China.
authors.forename: Zhu-Qing
authors.initials: ZQ
authors.lastname: Shao
authors.affiliation: Laboratory of Plant Genetics and Molecular Evolution, School of Life Sciences, Nanjing University, 163 XianLin Avenue, Nanjing, 210023, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.affiliation: Laboratory of Plant Genetics and Molecular Evolution, School of Life Sciences, Nanjing University, 163 XianLin Avenue, Nanjing, 210023, China.
authors.forename: Mian
authors.initials: M
authors.lastname: Wu
authors.affiliation: Laboratory of Plant Genetics and Molecular Evolution, School of Life Sciences, Nanjing University, 163 XianLin Avenue, Nanjing, 210023, China.
authors.forename: Cheng-Chen
authors.initials: CC
authors.lastname: Liu
authors.affiliation: Laboratory of Plant Genetics and Molecular Evolution, School of Life Sciences, Nanjing University, 163 XianLin Avenue, Nanjing, 210023, China.
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Wu
authors.affiliation: Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Huai'an, 223001, Jiangsu Province, China.
authors.forename: Jia-Yin
authors.initials: JY
authors.lastname: Yang
authors.affiliation: College of Agronomy, Northwest A & F University, Yangling, 712100, China.
authors.forename: De-Xiao
authors.initials: DX
authors.lastname: Li
authors.affiliation: Laboratory of Plant Genetics and Molecular Evolution, School of Life Sciences, Nanjing University, 163 XianLin Avenue, Nanjing, 210023, China. chenjq@nju.edu.cn.
authors.forename: Jian-Qun
authors.initials: JQ
authors.lastname: Chen
authors.affiliation: Laboratory of Plant Genetics and Molecular Evolution, School of Life Sciences, Nanjing University, 163 XianLin Avenue, Nanjing, 210023, China. binwang@nju.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
mesh_terms: D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D005799:Genes, Dominant; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D017800:Potyvirus; D013025:Glycine max / Q000235:genetics* / Q000821:virology
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers