title: Preliminary characterization of human skin microbiome in healthy Egyptian individuals.
abstract: Human skin is a large, complex ecosystem that harbors diverse microbial communities. The rapid advances in molecular techniques facilitate the exploration of skin associated bacterial populations. The objective of this study was to perform a preliminary characterization of skin associated bacterial populations in Egyptian individuals. Samples were collected from five healthy subjects from two skin sites; Antecubital Fossa (AF) and Popliteal Fossa (PF). Genomic DNA was extracted and used to amplify bacterial 16S rRNA genes which were sequenced on Illumina MiSeq platform. The two sites showed distinct diversity where PF was more diverse than AF. Taxonomic analysis of sequences revealed four main phyla Proteobacteria, Firmicutes, Actinobacteria and Deinococcus-Thermus, with Proteobacteria presenting the highest diversity. Klebsiella, Bacillus, Pseudomonas and Escherichia were the most predominant genera. Our data suggest that environmental factors can shape the composition of the skin microbiome in certain geographical regions. This study presents a new insight for subsequent analyses of human microbiome in Egypt.
authors.affiliation: Al-Azhar University Department of Microbiology and Immunology, College of Pharmacy Asyut Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Ramadan
authors.affiliation: Suez Canal University Department of Microbiology and Immunology, College of Pharmacy Ismailia Egypt.
authors.forename: S
authors.initials: S
authors.lastname: Solyman
authors.affiliation: Al-Azhar University Department of Microbiology and Immunology, College of Pharmacy Asyut Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Taha
authors.affiliation: Suez Canal University Department of Microbiology and Immunology, College of Pharmacy Ismailia Egypt ahanora@yahoo.com.
authors.forename: A
authors.initials: A
authors.lastname: Hanora
mesh_terms: D001419:Bacteria / Q000235:genetics; D044822:Biodiversity; D016000:Cluster Analysis; D004534:Egypt; D006262:Health; D006801:Humans; D064307:Microbiota / Q000235:genetics*; D010802:Phylogeny; D025341:Principal Component Analysis; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology*; D013045:Species Specificity