title: Self-initiated and concentration-dependent degradation of tetracaine in neat standard solutions: A trouble-shooting story.
abstract: This paper presents the trouble-shooting for a very unusual stability case. Tetracaine was found unstable in neat solutions only at high concentrations, but not at low concentrations. Moreover, its stable-isotope labeled internal standard did not show similar behavior. A series of trouble-shooting experiments were conducted to uncover the root cause. Some generally applicable precautions/insights can be drawn from this investigation to avoid potential stability issues during bioanalytical method development and validation.
authors.affiliation: Nucro-Technics, Scarborough, ON, Canada. Electronic address: tan@nucro-technics.com.
authors.forename: Aimin
authors.initials: A
authors.lastname: Tan
authors.affiliation: Nucro-Technics, Scarborough, ON, Canada.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Nucro-Technics, Scarborough, ON, Canada.
authors.forename: Guifen
authors.initials: G
authors.lastname: Gu
authors.affiliation: Nucro-Technics, Scarborough, ON, Canada.
authors.forename: John C
authors.initials: JC
authors.lastname: Fanaras
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards*; D004355:Drug Stability; D013058:Mass Spectrometry / Q000379:methods / Q000592:standards*; D012015:Reference Standards; D013748:Tetracaine / Q000032:analysis* / Q000737:chemistry*
chemical_list: D013748:Tetracaine
keywords: Bioanalysis; LC–MS; Stability; Stock solution; Tetracaine