title: Assessment of DNA damage in underground coal miners using the cytokinesis-block micronucleus assay in peripheral blood lymphocytes.
abstract: Coal miners are exposed to coal dust, containing mineral particles, inorganic compounds and polycyclic aromatic hydrocarbons, and to ionizing radiation. These factors can induce oxidative stress and promote inflammation that leads to DNA damage. The aim of this investigation is to analyse the degree of DNA damage in miners working in underground coal mines in Kemerovo Region (Russian Federation) using the cytokinesis-block micronucleus assay (CBMN) in peripheral blood lymphocytes. The exposed group included 143 coal miners (mean age = 50.11±7.36 years; mean length of service in coal mining conditions = 23.26±9.66 years). As a control group, we have used venous blood extracted from 127 healthy non-exposed men. The mean age in this group was 47.67±8.45 years. We have discovered that coal miners are characterized by a significant increase in the frequency of binucleated lymphocytes with micronuclei (MN), nucleoplasmic bridges (NPBs) and protrusions (NBUDs) compared to non-exposed donors. In addition, we report, for the first time, a reduction of cell proliferation in a cohort of coal miners. These data are evidence of the genotoxic and cytostatic effects of occupational harmful factors of the coal mining industry. No correlation between the level of chromosome damage and age, smoking status or length of service in coal mining conditions were discovered. We suggest that the CBMN assay would be useful in biomonitoring studies to monitor hygiene and prevention strategies in occupational settings in coal mining countries.
authors.affiliation: Department of Genetics, Kemerovo State University, Krasnaya St 6, 650043 Kemerovo, Russian Federation sinitsky.maxim@gmail.com.|Laboratory of Genome Medicine, Research Institute for Complex Issues of Cardiovascular Diseases, Sosnovy Boulevard 6, 650002 Kemerovo, Russian Federation.
authors.forename: Maxim Yu
authors.initials: MY
authors.lastname: Sinitsky
authors.affiliation: Department of Genetics, Kemerovo State University, Krasnaya St 6, 650043 Kemerovo, Russian Federation.|Laboratory of Cytogenetics, The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences, Leningradsky Ave 10, 650065 Kemerovo, Russian Federation.
authors.forename: Varvara I
authors.initials: VI
authors.lastname: Minina
authors.affiliation: Laboratory of Population Genetics, Research Institute for Complex Problems of Hygiene and Occupational Diseases, Kutuzova St 23, 654041 Novokuznetsk, Russian Federation.
authors.forename: Nikolay I
authors.initials: NI
authors.lastname: Gafarov
authors.affiliation: Laboratory of Cytogenetics, The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences, Leningradsky Ave 10, 650065 Kemerovo, Russian Federation.
authors.forename: Maxim A
authors.initials: MA
authors.lastname: Asanov
authors.affiliation: Department of Genetics, Kemerovo State University, Krasnaya St 6, 650043 Kemerovo, Russian Federation.
authors.forename: Aleksey V
authors.initials: AV
authors.lastname: Larionov
authors.affiliation: Laboratory of Genome Medicine, Research Institute for Complex Issues of Cardiovascular Diseases, Sosnovy Boulevard 6, 650002 Kemerovo, Russian Federation.
authors.forename: Anastasia V
authors.initials: AV
authors.lastname: Ponasenko
authors.affiliation: Department of Genetics, Kemerovo State University, Krasnaya St 6, 650043 Kemerovo, Russian Federation.
authors.forename: Valentin P
authors.initials: VP
authors.lastname: Volobaev
authors.affiliation: Department of Genetics, Kemerovo State University, Krasnaya St 6, 650043 Kemerovo, Russian Federation.|Laboratory of Cytogenetics, The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences, Leningradsky Ave 10, 650065 Kemerovo, Russian Federation.
authors.forename: Vladimir G
authors.initials: VG
authors.lastname: Druzhinin
mesh_terms: D000328:Adult; D003031:Coal / Q000633:toxicity; D004249:DNA Damage; D004391:Dust; D006801:Humans; D008214:Lymphocytes / Q000473:pathology*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D008875:Middle Aged; D000067572:Miners; D016273:Occupational Exposure; D012426:Russia
chemical_list: D003031:Coal; D004391:Dust