title: Different classes of hydrolytic enzymes produced by multidrug-resistant yeasts comprising the Candida haemulonii complex.
abstract: The production of enzymes in clinical isolates of Candida haemulonii (Ch, n = 5), Candida duobushaemulonii (Cd, n = 4) and Candida haemulonii var. vulnera (Chv, n = 3) was identified by agar plate. Aspartic protease, phytase, caseinolytic and hemolytic activities were detected in all the isolates. A distinct scenario was evidenced regarding the production of lipases. In this way, 80%, 50% and 100% of Ch, Cd and Chv, respectively, were phospholipase producers. Regarding esterase activity, 100%, 50% and 66.7% of Ch, Cd and Chv, respectively, were positive isolates. Esterase activity was significantly higher in isolates recovered from cutaneous candidiasis compared with those recovered from body fluids.
authors.affiliation: Laboratório de Investigação de Peptidases, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Lívia S
authors.initials: LS
authors.lastname: Ramos
authors.affiliation: Laboratório de Investigação de Peptidases, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Marta H
authors.initials: MH
authors.lastname: Branquinha
authors.affiliation: Laboratório de Investigação de Peptidases, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil andre@micro.ufrj.br.|Programa de Pós-Graduação em Bioquímica, Instituto de Química, UFRJ, Rio de Janeiro, Brazil.
authors.forename: André L S
authors.initials: AL
authors.lastname: Santos
mesh_terms: D002175:Candida / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology*; D003470:Culture Media; D026141:Drug Resistance, Multiple, Fungal; D006801:Humans; D006867:Hydrolases / Q000032:analysis*; D008828:Microbiological Techniques
chemical_list: D003470:Culture Media; D006867:Hydrolases
keywords: Brazilian clinical isolates; Candida haemulonii complex; Cutaneous infection; Fluid isolates; Hydrolytic enzymes; Virulence attributes