title: CD19+ B cell subsets in the peripheral blood and skin lesions of psoriasis patients and their correlations with disease severity.
abstract: T lymphocytes are important in the pathogenesis of psoriasis, and increasing evidence indicates that B cells also play an important role. The mechanisms of action, however, remain unclear. We evaluated the ratios of CD19+ B cells in peripheral blood mononuclear cells (PBMCs) from 157 patients with psoriasis (65 patients with psoriasis vulgaris, 32 patients with erythrodermic psoriasis, 30 patients with arthropathic psoriasis, and 30 patients with pustular psoriasis) and 35 healthy controls (HCs). Ratios of CD19+ B cells in skin lesions were compared with non-lesions in 7 erythrodermic psoriasis patients. The Psoriasis Area Severity Index (PASI) was used to measure disease severity. CD19+ B cell ratios in PBMCs from psoriasis vulgaris (at both the active and stationary stage) and arthropathic psoriasis patients were higher compared with HCs (P<0.01), but ratios were lower in erythrodermic and pustular psoriasis patients (P<0.01). CD19+ B cell ratios in erythrodermic psoriasis skin lesions were higher than in non-lesion areas (P<0.001). Different subsets of CD19+CD40+, CD19+CD44+, CD19+CD80+, CD19+CD86+, CD19+CD11b+, and CD19+HLA-DR+ B cells in PBMCs were observed in different psoriasis clinical subtypes. PASI scores were positively correlated with CD19+ B cell ratios in psoriasis vulgaris and arthropathic psoriasis cases (r=0.871 and r=0.692, respectively, P<0.01), but were negatively correlated in pustular psoriasis (r=-0.569, P<0.01). The results indicated that similar to T cells, B cells activation may also play important roles in different pathological stages of psoriasis.
authors.affiliation: Shanghai Skin Diseases Hospital, Shanghai, China.
authors.forename: J
authors.initials: J
authors.lastname: Lu
authors.affiliation: Shanghai Skin Diseases Hospital, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Shanghai Skin Diseases Hospital, Shanghai, China.
authors.forename: X
authors.initials: X
authors.lastname: Yi
authors.affiliation: Department of Dermatology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: J
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000328:Adult; D000368:Aged; D018941:Antigens, CD19 / Q000097:blood* / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000097:blood* / Q000276:immunology; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D018941:Antigens, CD19; D015415:Biomarkers