title: In vivo white light and contrast-enhanced vital-dye fluorescence imaging of Barrett's-related neoplasia in a single-endoscopic insertion.
abstract: A modular video endoscope is developed to enable both white light imaging (WLI) and vital-dye fluorescence imaging (VFI) in a single-endoscopic insertion for the early detection of cancer in Barrett’s esophagus (BE). We demonstrate that VFI can be achieved in conjunction with white light endoscopy, where appropriate white balance is used to correct for the presence of the emission filter. In VFI mode, a contrast enhancement feature is implemented in real time to further highlight glandular patterns in BE and related malignancies without introducing artifacts. In a pilot study, we demonstrate accurate correlation of images in two widefield modalities, with representative images showing the disruption and effacement of glandular architecture associated with cancer development in BE. VFI images of these alterations exhibit enhanced contrast when compared to WLI. Results suggest that the usefulness of VFI in the detection of BE-related neoplasia should be further evaluated in future in vivo studies.
authors.affiliation: Rice University, Department of Bioengineering, 6100 Main Street, MS 142, Houston, Texas 77005, United States.
authors.forename: Yubo
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Rice University, Department of Bioengineering, 6100 Main Street, MS 142, Houston, Texas 77005, United States.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Carns
authors.affiliation: Mount Sinai Medical Center, Department of Pathology, One Gustave L. Levy Place, Box 1194, New York, New York 10029-6574, United States.
authors.forename: Alexandros D
authors.initials: AD
authors.lastname: Polydorides
authors.affiliation: Baylor St. Luke's Medical Center, 7200 Cambridge Street, Suite 10C, Houston, Texas 77030, United States.
authors.forename: Sharmila
authors.initials: S
authors.lastname: Anandasabapathy
authors.affiliation: Rice University, Department of Bioengineering, 6100 Main Street, MS 142, Houston, Texas 77005, United States.
authors.forename: Rebecca R
authors.initials: RR
authors.lastname: Richards-Kortum
mesh_terms: D000818:Animals; D001471:Barrett Esophagus / Q000000981:diagnostic imaging; D004938:Esophageal Neoplasms / Q000000981:diagnostic imaging*; D004945:Esophagoscopy / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D061848:Optical Imaging; D010865:Pilot Projects