title: A comparison of electrochemically pre-treated and spark-platinized carbon fiber microelectrode. Measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine in human urine and plasma.
abstract: A novel method of carbon fiber microelectrode activation using spark discharge was demonstrated and compared to conventional electrochemical pretreatment by potential cycling. The spark discharge was performed at 800 V between the microelectrode connected to positive pole of the power supply and platinum counter electrode. Spark discharge led both to trimming of the fiber tip into conical shape and to the modification of carbon fiber microelectrode with platinum, as proven by scanning electron microscopy and electron dispersive X-ray spectroscopy. After the characterization of electrochemical properties using ferricyanide voltammetry, the activated electrodes were used for electrochemical analysis of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an oxidative stress marker. Subnanomolar detection limits (0.55 nmol L(-1)) in high-performance liquid chromatography were achieved for spark platinized electrodes incorporated into the flow detection cell.
authors.affiliation: Department of Analytical Chemistry, Palacky University, Faculty of Science, 17.listopadu 12, CZ-771 46 Olomouc, Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Bartosova
authors.affiliation: Department of Analytical Chemistry, Palacky University, Faculty of Science, 17.listopadu 12, CZ-771 46 Olomouc, Czech Republic.
authors.forename: D
authors.initials: D
authors.lastname: Riman
authors.affiliation: Department of Analytical Chemistry, Palacky University, Faculty of Science, 17.listopadu 12, CZ-771 46 Olomouc, Czech Republic; Department of Physics and Materials Engineering, Faculty of Technology, Tomas Bata University in Zlin, nam. T.G. Masaryka 275, CZ-76001 Zlin, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Halouzka
authors.affiliation: Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, CZ-775 15 Olomouc, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Vostalova
authors.affiliation: Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, CZ-775 15 Olomouc, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Simanek
authors.affiliation: Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic. Electronic address: jhrbac@atlas.cz.
authors.forename: J
authors.initials: J
authors.lastname: Hrbac
authors.affiliation: Department of Analytical Chemistry, Palacky University, Faculty of Science, 17.listopadu 12, CZ-771 46 Olomouc, Czech Republic. Electronic address: david.jirovsky@upol.cz.
authors.forename: D
authors.initials: D
authors.lastname: Jirovsky
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D002244:Carbon / Q000737:chemistry*; D000077482:Carbon Fiber; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D055664:Electrochemical Techniques; D006801:Humans; D057446:Hydrodynamics; D008839:Microelectrodes; D010316:Particle Size
chemical_list: D000077482:Carbon Fiber; D002244:Carbon; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-oxo-7,8-dihydro-2′-deoxyguanosine; Amperometric detection; Carbon fiber microelectrode; Liquid chromatography; Platinization; Spark discharge