title: Skin Treatment with Pulsed Monochromatic UVA1 355 Device and Computerized Morphometric Analysis of Histochemically Identified Langerhans Cells.
abstract: Fluorescent or metal halide lamps are widely used in therapeutic applications in dermatological diseases, with broadband or narrow band emission UVA/UVA1 (320-400 nm) obtained with suitable passive filters. Recently, it has been possible for us to use a new machine provided with solid state source emitting pulsed monochromatic UVA1 355 nm. In order to evaluate the effects of this emission on immunocells of the skin, human skin samples were irradiated with monochromatic 355 nm UVA1 with different energetic fluences and after irradiation Langerhans cells were labeled with CD1a antibodies. The immunohistochemical identification of these cells permitted evaluating their modifications in terms of density into the skin. Obtained results are promising for therapeutical applications, also considering that a monochromatic radiation minimizes thermic load and DNA damage in the skin tissues.
authors.affiliation: Department of Surgical and Morphological Sciences, University of Insubria, 21100 Varese, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Zerbinati
authors.affiliation: Department of Public Health, Experimental and Forensic Medicine, Histology and Embryology Unit, University of Pavia, 27100 Pavia, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Riva
authors.affiliation: Department of Molecular Medicine, University of Pavia and IRCCS San Matteo, 27100 Pavia, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Paulli
authors.affiliation: Department of Medical, Experimental and Clinical Sciences, University of Udine, 33100 Udine, Italy.
authors.forename: Pier Camillo
authors.initials: PC
authors.lastname: Parodi
authors.affiliation: Department of Public Health, Experimental and Forensic Medicine, Histology and Embryology Unit, University of Pavia, 27100 Pavia, Italy.
authors.forename: Alberto
authors.identifier: 0000-0002-8245-0704
authors.initials: A
authors.lastname: Calligaro
mesh_terms: D018949:Antigens, CD1 / Q000737:chemistry; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D005143:Eyelids / Q000601:surgery; D005453:Fluorescence; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000166:cytology*; D007834:Lasers; D012867:Skin / Q000276:immunology / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014467:Ultraviolet Therapy / Q000295:instrumentation* / Q000379:methods
chemical_list: D018949:Antigens, CD1; C419393:CD1a antigen