title: Genome-Wide Association Study in Wheat Identifies Resistance to the Cereal Cyst Nematode Heterodera filipjevi.
abstract: The cyst nematode Heterodera filipjevi is a plant parasite causing substantial yield loss in wheat. Resistant cultivars are the preferred method of controlling cyst nematodes. Association mapping is a powerful approach to detect associations between phenotypic variation and genetic polymorphisms; in this way favorable traits such as resistance to pathogens can be located. Therefore, a genome-wide association study of 161 winter wheat accessions was performed with a 90K iSelect single nucleotide polymorphism (SNP) chip. Population structure analysis grouped into two major subgroups and first principal component accounted 6.16% for phenotypic diversity. The genome-wide linkage disequilibrium across wheat was 3 cM. Eleven quantitative trait loci (QTLs) on chromosomes 1AL, 2AS, 2BL, 3AL, 3BL, 4AS, 4AL, 5BL, and 7BL were identified using a mixed linear model false discovery rate of P < 0.01 that explained 43% of total genetic variation. This is the first report of QTLs conferring resistance to H. filipjevi in wheat. Eight QTLs on chromosomes 1AL, 2AS, 2BL, 3AL, 4AL, and 5BL were linked to putative genes known to be involved in plant-pathogen interactions. Two other QTLs on 3BL and one QTL on 7BL linked to putative genes known to be involved in abiotic stress.
authors.affiliation: First, fifth, sixth, and ninth authors: Institute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, Karlrobert-Kreiten Strasse 13, D-53115 Bonn, Germany; second, fourth, and seventh authors: International Maize and Wheat Improvement Centre (CIMMYT), P.K. 39 06511, Emek, Ankara, Turkey; third and eighth authors: Institute of Crop Science and Resource Conservation (INRES), Plant Breeding, Katzenburgweg; and fifth author: Agricultural Research Center (ARC), Agricultural genetic Engineering Research Institute (AGERI), 9 Gama Street, Giza 12619, Egypt.
authors.forename: Shree R
authors.initials: SR
authors.lastname: Pariyar
authors.affiliation: First, fifth, sixth, and ninth authors: Institute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, Karlrobert-Kreiten Strasse 13, D-53115 Bonn, Germany; second, fourth, and seventh authors: International Maize and Wheat Improvement Centre (CIMMYT), P.K. 39 06511, Emek, Ankara, Turkey; third and eighth authors: Institute of Crop Science and Resource Conservation (INRES), Plant Breeding, Katzenburgweg; and fifth author: Agricultural Research Center (ARC), Agricultural genetic Engineering Research Institute (AGERI), 9 Gama Street, Giza 12619, Egypt.
authors.forename: Abdelfattah A
authors.initials: AA
authors.lastname: Dababat
authors.affiliation: First, fifth, sixth, and ninth authors: Institute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, Karlrobert-Kreiten Strasse 13, D-53115 Bonn, Germany; second, fourth, and seventh authors: International Maize and Wheat Improvement Centre (CIMMYT), P.K. 39 06511, Emek, Ankara, Turkey; third and eighth authors: Institute of Crop Science and Resource Conservation (INRES), Plant Breeding, Katzenburgweg; and fifth author: Agricultural Research Center (ARC), Agricultural genetic Engineering Research Institute (AGERI), 9 Gama Street, Giza 12619, Egypt.
authors.forename: Wiebke
authors.initials: W
authors.lastname: Sannemann
authors.affiliation: First, fifth, sixth, and ninth authors: Institute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, Karlrobert-Kreiten Strasse 13, D-53115 Bonn, Germany; second, fourth, and seventh authors: International Maize and Wheat Improvement Centre (CIMMYT), P.K. 39 06511, Emek, Ankara, Turkey; third and eighth authors: Institute of Crop Science and Resource Conservation (INRES), Plant Breeding, Katzenburgweg; and fifth author: Agricultural Research Center (ARC), Agricultural genetic Engineering Research Institute (AGERI), 9 Gama Street, Giza 12619, Egypt.
authors.forename: Gul
authors.initials: G
authors.lastname: Erginbas-Orakci
authors.affiliation: First, fifth, sixth, and ninth authors: Institute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, Karlrobert-Kreiten Strasse 13, D-53115 Bonn, Germany; second, fourth, and seventh authors: International Maize and Wheat Improvement Centre (CIMMYT), P.K. 39 06511, Emek, Ankara, Turkey; third and eighth authors: Institute of Crop Science and Resource Conservation (INRES), Plant Breeding, Katzenburgweg; and fifth author: Agricultural Research Center (ARC), Agricultural genetic Engineering Research Institute (AGERI), 9 Gama Street, Giza 12619, Egypt.
authors.forename: Abdelnaser
authors.initials: A
authors.lastname: Elashry
authors.affiliation: First, fifth, sixth, and ninth authors: Institute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, Karlrobert-Kreiten Strasse 13, D-53115 Bonn, Germany; second, fourth, and seventh authors: International Maize and Wheat Improvement Centre (CIMMYT), P.K. 39 06511, Emek, Ankara, Turkey; third and eighth authors: Institute of Crop Science and Resource Conservation (INRES), Plant Breeding, Katzenburgweg; and fifth author: Agricultural Research Center (ARC), Agricultural genetic Engineering Research Institute (AGERI), 9 Gama Street, Giza 12619, Egypt.
authors.forename: Shahid
authors.initials: S
authors.lastname: Siddique
authors.affiliation: First, fifth, sixth, and ninth authors: Institute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, Karlrobert-Kreiten Strasse 13, D-53115 Bonn, Germany; second, fourth, and seventh authors: International Maize and Wheat Improvement Centre (CIMMYT), P.K. 39 06511, Emek, Ankara, Turkey; third and eighth authors: Institute of Crop Science and Resource Conservation (INRES), Plant Breeding, Katzenburgweg; and fifth author: Agricultural Research Center (ARC), Agricultural genetic Engineering Research Institute (AGERI), 9 Gama Street, Giza 12619, Egypt.
authors.forename: Alexei
authors.initials: A
authors.lastname: Morgounov
authors.affiliation: First, fifth, sixth, and ninth authors: Institute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, Karlrobert-Kreiten Strasse 13, D-53115 Bonn, Germany; second, fourth, and seventh authors: International Maize and Wheat Improvement Centre (CIMMYT), P.K. 39 06511, Emek, Ankara, Turkey; third and eighth authors: Institute of Crop Science and Resource Conservation (INRES), Plant Breeding, Katzenburgweg; and fifth author: Agricultural Research Center (ARC), Agricultural genetic Engineering Research Institute (AGERI), 9 Gama Street, Giza 12619, Egypt.
authors.forename: Jens
authors.initials: J
authors.lastname: Leon
authors.affiliation: First, fifth, sixth, and ninth authors: Institute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, Karlrobert-Kreiten Strasse 13, D-53115 Bonn, Germany; second, fourth, and seventh authors: International Maize and Wheat Improvement Centre (CIMMYT), P.K. 39 06511, Emek, Ankara, Turkey; third and eighth authors: Institute of Crop Science and Resource Conservation (INRES), Plant Breeding, Katzenburgweg; and fifth author: Agricultural Research Center (ARC), Agricultural genetic Engineering Research Institute (AGERI), 9 Gama Street, Giza 12619, Egypt.
authors.forename: Florian M W
authors.initials: FM
authors.lastname: Grundler
mesh_terms: D000818:Animals; D032461:Chromosomes, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D002523:Edible Grain / Q000276:immunology / Q000469:parasitology; D055106:Genome-Wide Association Study; D016014:Linear Models; D015810:Linkage Disequilibrium; D010641:Phenotype; D010935:Plant Diseases / Q000276:immunology* / Q000469:parasitology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D014431:Tylenchoidea / Q000502:physiology*