title: A New Tetraphenylethylene-Derived Fluorescent Probe for Nitroreductase Detection and Hypoxic-Tumor-Cell Imaging.
abstract: The fluorescence detection of nitroreductase (NTR) and evaluation of the hypoxia status of tumor cells are vital, not only for clinical diagnoses and therapy, but also for biomedical research. Herein, we report the synthesis and application of a new fluorometric "turn-on" probe for the detection of NTR (TPE-NO2) that takes advantage of the aggregation-induced emission of tetraphenylethylene. TPE-NO2can detect NTR at concentrations as low as 5 ng mL-1in aqueous solution. The detection mechanism relied on the aggregation and deaggregation of tetraphenylethylene molecules. Moreover, this fluorescent probe can be used to monitor the hypoxia status of tumor cells through the detection of endogenous NTR.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Laboratories of Organic Solids and Analytical Chemistry for Living, Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Xue
authors.initials: X
authors.lastname: You
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Laboratories of Organic Solids and Analytical Chemistry for Living, Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Li
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Laboratories of Organic Solids and Analytical Chemistry for Living, Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Laboratories of Organic Solids and Analytical Chemistry for Living, Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Laboratories of Organic Solids and Analytical Chemistry for Living, Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China. gxzhang@iccas.ac.cn.
authors.forename: Guanxin
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Laboratories of Organic Solids and Analytical Chemistry for Living, Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China. dqzhang@iccas.ac.cn.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China. dqzhang@iccas.ac.cn.
authors.forename: Deqing
authors.initials: D
authors.lastname: Zhang
mesh_terms: D055162:Biocatalysis; D003287:Contrast Media / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D009243:NAD / Q000737:chemistry; D009585:Nitrogen Dioxide / Q000737:chemistry; D009601:Nitroreductases / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013267:Stilbenes / Q000737:chemistry* / Q000378:metabolism; D000072258:Tumor Hypoxia
chemical_list: D003287:Contrast Media; D005456:Fluorescent Dyes; D013267:Stilbenes; C000617116:tetraphenylethylene; D009243:NAD; D009601:Nitroreductases; D009585:Nitrogen Dioxide
keywords: aggregation; fluorescence; imaging agents; nitroreductase; tetraphenylethylene