title: Periconceptional undernutrition affects in utero methyltransferase expression and steroid hormone concentrations in uterine flushings and blood plasma during the peri-implantation period in domestic pigs.
abstract: Female undernutrition during early pregnancy may affect the physiological pattern of genomic DNA methylation. We hypothesised that in utero DNA methylation may be impaired in females fed a restrictive diet in early pregnancy. In this study we evaluated whether poor maternal nutritional status, induced by applying a restricted diet during the peri-conceptional period, may influence: (1) the potential for in utero DNA methylation, expressed as changes in the mRNA expression and protein abundance of methyltransferases: DNA methyltransferase 1 (DNMT1) and DNMT3a in the endometrium and the myometrium, (2) the intrauterine microenvironment, measured as oestradiol 17β (E2) and progesterone (P4) concentrations in uterine flushings and (3) plasma concentration of E2and P4during the peri-implantation period. Our results indicate that maternal peri-conceptional undernutrition affects maintenance and de novo DNA methylation in the endometrium, de novo methylation in the myometrium and a results in a decrease in intrauterine E2concentration during the peri-implantation period. The intrauterine concentration of P4and plasma concentrations of E2and P4did not change. These findings suggest that undernutrition during the earliest period of pregnancy, and perhaps the pre-pregnancy period, may create changes in epigenetic mechanisms in the uterus and intrauterine milieu of E2during the peri-implantation period.
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-718 Olsztyn, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Franczak
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-718 Olsztyn, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Zglejc
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-718 Olsztyn, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Waszkiewicz
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-718 Olsztyn, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Wojciechowicz
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-718 Olsztyn, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Martyniak
authors.affiliation: Department of Animal Nutrition and Feed Management, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowski 5, 10-718 Olsztyn, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Sobotka
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-718 Olsztyn, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Okrasa
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-718 Olsztyn, Poland.
authors.forename: G
authors.initials: G
authors.lastname: Kotwica
mesh_terms: D000818:Animals; D000076123:DNA (Cytosine-5-)-Methyltransferase 1 / Q000235:genetics / Q000378:metabolism*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D010064:Embryo Implantation / Q000502:physiology*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D044342:Malnutrition / Q000235:genetics / Q000378:metabolism*; D039382:Maternal Nutritional Physiological Phenomena / Q000502:physiology*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D013552:Swine; D014599:Uterus / Q000378:metabolism*
chemical_list: D011374:Progesterone; D004958:Estradiol; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases