title: HAPLESS13-Mediated Trafficking of STRUBBELIG Is Critical for Ovule Development in Arabidopsis.
abstract: Planar morphogenesis, a distinct feature of multicellular organisms, is crucial for the development of ovule, progenitor of seeds. Both receptor-like kinases (RLKs) such as STRUBBELIG (SUB) and auxin gradient mediated by PIN-FORMED1 (PIN1) play instructive roles in this process. Fine-tuned intercellular communications between different cell layers during ovule development demands dynamic membrane distribution of these cell-surface proteins, presumably through vesicle-mediated sorting. However, the way it's achieved and the trafficking routes involved are obscure. We report that HAPLESS13 (HAP13)-mediated trafficking of SUB is critical for ovule development. HAP13 encodes the μ subunit of adaptor protein 1 (AP1) that mediates protein sorting at the trans-Golgi network/early endosome (TGN/EE). The HAP13 mutant, hap13-1, is defective in outer integument growth, resulting in exposed nucellus accompanied with impaired pollen tube guidance and reception. SUB is mis-targeted in hap13-1. However, unlike that of PIN2, the distribution of PIN1 is independent of HAP13. Genetic interference of exocytic trafficking at the TGN/EE by specifically downregulating HAP13 phenocopied the defects of hap13-1 in SUB targeting and ovule development, supporting a key role of sporophytically expressed SUB in instructing female gametogenesis.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, China.
authors.forename: Jia-Gang
authors.initials: JG
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, China.
authors.forename: Hai-Hong
authors.initials: HH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, China.
authors.forename: Fu-Rong
authors.initials: FR
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Hong-Ju
authors.identifier: 0000-0003-0064-4626
authors.initials: HJ
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D033961:Adaptor Protein Complex 1 / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics*; D011992:Endosomes / Q000235:genetics; D056130:Gametogenesis, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D026161:MADS Domain Proteins / Q000096:biosynthesis / Q000235:genetics; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000235:genetics*; D056771:Ovule / Q000235:genetics* / Q000254:growth & development; D063245:Plant Development / Q000235:genetics; D021381:Protein Transport / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction
chemical_list: D033961:Adaptor Protein Complex 1; D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D026161:MADS Domain Proteins; D026901:Membrane Transport Proteins; D020794:Receptor Protein-Tyrosine Kinases; C091535:AP1 protein, Arabidopsis; C583122:AP1M2 protein, Arabidopsis; C116363:PIN1 protein, Arabidopsis; C117013:AT5G57090 protein, Arabidopsis; C503317:SUB protein, Arabidopsis