title: Population Genetic Structure and Marker Trait Associations Using Morphological, Phytochemical and Molecular Parameters in Habenaria edgeworthii-a Threatened Medicinal Orchid of West Himalaya, India.
abstract: Habenaria edgeworthii Hook. f. ex Collett is an important terrestrial orchid used in different Ayurvedic formulations. In the present study, variations among morphological, phytochemical and molecular markers were assessed. A significant difference was observed among populations using morphological traits. Inter-simple sequence repeat (ISSR) data revealed lower genetic diversity at population level (He = 0.207) as compared to species level (He = 0.334). Analysis of molecular variance (AMOVA) indicates 74 % variation among populations and 26 % within population. Tuber extracts showed significantly (p < 0.05) higher total phenolics and flavonoids among the populations. Antioxidant activity determined by 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) assays exhibited considerable antioxidant potential. Furthermore, the associations between molecular and morphological and phytochemical attributes were studied using multiple regression analysis (MRA). Several ISSR fragments were associated with some morphological and phytochemical traits. These ISSR fragments can be useful for breeding programme of the species when no other genetic information, such as linkage maps and quantitative trait loci, is available.
authors.affiliation: G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India.
authors.forename: Lalit
authors.initials: L
authors.lastname: Giri
authors.affiliation: G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India.
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Jugran
authors.affiliation: G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Bahukhandi
authors.affiliation: G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Dhyani
authors.affiliation: G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India. id_bhatt@yahoo.com.
authors.forename: Indra D
authors.initials: ID
authors.lastname: Bhatt
authors.affiliation: G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India.
authors.forename: Ranbeer Singh
authors.initials: RS
authors.lastname: Rawal
authors.affiliation: G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India.
authors.forename: Shyamal Kumar
authors.initials: SK
authors.lastname: Nandi
authors.affiliation: University School of Environment Management, GGS Indraprastha University, Sector-16C, Dwarka, New Delhi, 110078, India.
authors.forename: Uppeandra
authors.initials: U
authors.lastname: Dhar
mesh_terms: D000975:Antioxidants / Q000378:metabolism / Q000627:therapeutic use; D005419:Flavonoids / Q000378:metabolism / Q000627:therapeutic use; D005828:Genetics, Population; D006801:Humans; D007194:India; D008515:Medicine, Ayurvedic; D018895:Microsatellite Repeats / Q000235:genetics*; D029595:Orchidaceae / Q000235:genetics* / Q000254:growth & development; D010636:Phenols / Q000378:metabolism / Q000627:therapeutic use; D064209:Phytochemicals / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D064209:Phytochemicals; D010936:Plant Extracts
keywords: ISSR analysis; Medicinal plant; Phytochemical; Trait association