title: N-glycosylation proteomic characterization and cross-species comparison of milk fat globule membrane proteins from mammals.
abstract: Glycosylation of proteins has been implicated in various biological functions and has received much attention; however, glycoprotein components and inter-species complexity have not yet been elucidated fully in milk proteins. N-linked glycosylation sites and glycoproteins in milk fat globule membrane (MFGM) fractions were investigated by combining N-glycosylated peptides enrichment and high-accuracy Q Exactive identification, to map the N-glycoproteome profiles in Holstein and Jersey cows, buffaloes, yaks, goats, camels, horses, and humans. A total of 399 N-glycoproteins with 677 glycosylation sites were identified in the MFGM fractions of the studied mammals. Most glycosylation sites in humans were classified as known and those in the other studied mammals as unknown, according to Swiss-Prot annotations. Functionally, most of the identified glycoproteins were associated with the 'response to stimulus' GO category. N-glycosylated protein components of MFGM fractions from Holstein and Jersey cows, buffaloes, yaks, and goats were more similar to each other compared with those of camels, horses and human. The findings increased the number of known N-glycosylation sites in the milk from dairy animal species, revealed the complexity of the MFGM glycoproteome, and provided useful information to further explore the mechanism of MFGM glycoproteins biosynthesis among the studied mammals.
authors.affiliation: Ministry of Agriculture-Milk Risk Assessment Laboratory, State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.|Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Ministry of Agriculture-Milk Risk Assessment Laboratory, State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.affiliation: The High School affiliated to Renmin University of China, Beijing, China.
authors.forename: Weiyu
authors.initials: W
authors.lastname: Wang
authors.affiliation: Ministry of Agriculture-Milk Risk Assessment Laboratory, State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.|Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Ministry of Agriculture-Milk Risk Assessment Laboratory, State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Yangdong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Ministry of Agriculture-Milk Risk Assessment Laboratory, State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Rongwei
authors.initials: R
authors.lastname: Han
authors.affiliation: Ministry of Agriculture-Milk Risk Assessment Laboratory, State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Ma
authors.affiliation: Ministry of Agriculture-Milk Risk Assessment Laboratory, State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Shengguo
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Ministry of Agriculture-Milk Risk Assessment Laboratory, State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Songli
authors.initials: S
authors.lastname: Li
authors.affiliation: Ministry of Agriculture-Milk Risk Assessment Laboratory, State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Tongjun
authors.initials: T
authors.lastname: Guo
authors.affiliation: Ministry of Agriculture-Milk Risk Assessment Laboratory, State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Zang
authors.affiliation: Ministry of Agriculture-Milk Risk Assessment Laboratory, State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China. wangjiaqi@caas.cn.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D002162:Camelus / Q000235:genetics; D002417:Cattle; D030562:Databases, Protein; D005260:Female; D006017:Glycolipids / Q000737:chemistry / Q000235:genetics*; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006031:Glycosylation; D006041:Goats / Q000235:genetics; D006736:Horses; D006801:Humans; D066292:Lipid Droplets; D008565:Membrane Proteins; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000737:chemistry / Q000235:genetics*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D006017:Glycolipids; D006023:Glycoproteins; D008565:Membrane Proteins; D008894:Milk Proteins; C110367:milk fat globule
keywords: Animal proteomics; Animal species; Glycosylation; Milk fat globule membrane; Proteome