title: Effects of Mechanical Stretch on Cell Proliferation and Matrix Formation of Mesenchymal Stem Cell and Anterior Cruciate Ligament Fibroblast.
abstract: Mesenchymal stem cells (MSCs) and fibroblasts are two major seed cells for ligament tissue engineering. To understand the effects of mechanical stimulation on these cells and to develop effective approaches for cell therapy, it is necessary to investigate the biological effects of various mechanical loading conditions on cells. In this study, fibroblasts and MSCs were tested and compared under a novel Uniflex/Bioflex culture system that might mimic mechanical strain in ligament tissue. The cells were uniaxially or radially stretched with different strains (5%, 10%, and 15%) at 0.1, 0.5, and 1.0 Hz. The cell proliferation and collagen production were compared to find the optimal parameters. The results indicated that uniaxial stretch (15% at 0.5 Hz; 10% at 1.0 Hz) showed positive effects on fibroblast. The uniaxial strains (5%, 10%, and 15%) at 0.5 Hz and 10% strain at 1.0 Hz were favorable for MSCs. Radial strain did not have significant effect on fibroblast. On the contrary, the radial strains (5%, 10%, and 15%) at 0.1 Hz had positive effects on MSCs. This study suggested that fibroblasts and MSCs had their own appropriate mechanical stimulatory parameters. These specific parameters potentially provide fundamental knowledge for future cell-based ligament regeneration.
authors.affiliation: Department of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China; Tianjin Sanatorium, Beijing Military Region, Tianjin 300381, China.
authors.forename: Liguo
authors.identifier: 0000-0001-5848-4181
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Clinical Laboratory, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Qu
authors.affiliation: College of Science, Air Force Engineering University, Xi'an 710051, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Department of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Hongguo
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yingsen
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xincheng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Division of Advanced Prosthodontics, School of Dentistry, University of California, Los Angeles, CA 90095, USA.
authors.forename: Jiabing
authors.identifier: 0000-0002-7485-8275
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Hongbin
authors.identifier: 0000-0002-7656-9530
authors.initials: H
authors.lastname: Fan