title: Utility of Ochrobactrum anthropi YC152 in a Microbial Fuel Cell as an Early Warning Device for Hexavalent Chromium Determination.
abstract: Fast hexavalent chromium (Cr(VI)) determination is important for environmental risk and health-related considerations. We used a microbial fuel cell-based biosensor inoculated with a facultatively anaerobic, Cr(VI)-reducing, and exoelectrogenic Ochrobactrum anthropi YC152 to determine the Cr(VI) concentration in water. The results indicated that O. anthropi YC152 exhibited high adaptability to pH, temperature, salinity, and water quality under anaerobic conditions. The stable performance of the microbial fuel cell (MFC)-based biosensor indicated its potential as a reliable biosensor system. The MFC voltage decreased as the Cr(VI) concentration in the MFC increased. Two satisfactory linear relationships were observed between the Cr(VI) concentration and voltage output for various Cr(VI) concentration ranges (0.0125-0.3 mg/L and 0.3-5 mg/L). The MFC biosensor is a simple device that can accurately measure Cr(VI) concentrations in drinking water, groundwater, and electroplating wastewater in 45 min with low deviations (<10%). The use of the biosensor can help in preventing the violation of effluent regulations and the maximum allowable concentration of Cr(VI) in water. Thus, the developed MFC biosensor has potential as an early warning detection device for Cr(VI) determination even if O. anthropi YC152 is a possible opportunistic pathogen.
authors.affiliation: Fujian Provincial Key Laboratory of Biological Engineering on Traditional Herbs and Research Center of Natural Cosmeceuticals Engineering, Xiamen Medical College, Xiamen 361008, China. wanggh@livemail.tw.
authors.forename: Guey-Horng
authors.initials: GH
authors.lastname: Wang
authors.affiliation: Department of Biological Science and Technology, China University of Science and Technology, Taipei 11581, Taiwan. cycheng@cc.cust.edu.tw.
authors.forename: Chiu-Yu
authors.initials: CY
authors.lastname: Cheng
authors.affiliation: Department of Food Science, China University of Science and Technology, Taipei 11581, Taiwan. manhailiu@cc.cust.edu.tw.
authors.forename: Man-Hai
authors.initials: MH
authors.lastname: Liu
authors.affiliation: Department of Biological Science and Technology, China University of Science and Technology, Taipei 11581, Taiwan. HBO223080284@gmail.com.
authors.forename: Tzu-Yu
authors.initials: TY
authors.lastname: Chen
authors.affiliation: Department of Biological Science and Technology, China University of Science and Technology, Taipei 11581, Taiwan. tsakaguchi22@gmail.com.
authors.forename: Min-Chi
authors.initials: MC
authors.lastname: Hsieh
authors.affiliation: Department of Biological Science and Technology, China University of Science and Technology, Taipei 11581, Taiwan. ycchung@cc.cust.edu.tw.
authors.forename: Ying-Chien
authors.initials: YC
authors.lastname: Chung
mesh_terms: D000693:Anaerobiosis; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D015374:Biosensing Techniques / Q000379:methods*; D002857:Chromium / Q000302:isolation & purification* / Q000633:toxicity; D020621:Ochrobactrum anthropi / Q000737:chemistry* / Q000235:genetics; D012722:Sewage / Q000737:chemistry / Q000382:microbiology; D062065:Wastewater / Q000737:chemistry; D018508:Water Purification / Q000379:methods
chemical_list: D002857:Chromium; D012722:Sewage; D062065:Wastewater; C074702:chromium hexavalent ion
keywords: biosensor; chromiun; microbial fuel cell; wastewater