title: Karrikins Identified in Biochars Indicate Post-Fire Chemical Cues Can Influence Community Diversity and Plant Development.
abstract: BACKGROUND
  Karrikins are smoke-derived compounds that provide strong chemical cues to stimulate seed germination and seedling growth. The recent discovery in Arabidopsis that the karrikin perception system may be present throughout angiosperms implies a fundamental plant function. Here, we identify the most potent karrikin, karrikinolide (KAR1), in biochars and determine its role in species unique plant responses.
  
  
  METHODS
  Biochars were prepared by three distinct commercial-scale pyrolysis technologies using systematically selected source material and their chemical properties, including karrikinolide, were quantified. Dose-response assays determined the effects of biochar on seed germination for two model species that require karrikinolide to break dormancy (Solanum orbiculatum, Brassica tourneforttii) and on seedling growth using two species that display plasticity to karrikins, biochar and phytotoxins (Lactuca sativa, Lycopersicon esculentum). Multivariate analysis examined relationships between biochar properties and the plant phenotype.
  
  
  CONCLUSIONS
  Results showed that karrikin abundant biochars stimulated dormant seed germination and seedling growth via mechanisms analogous to post-fire chemical cues. The individual species response was associated with its sensitivity to karrikinolide and inhibitory compounds within the biochars. These findings are critical for understanding why biochar influences community composition and plant physiology uniquely for different species and reaffirms that future pyrolysis technologies promise by-products that concomitantly sequester carbon and enhance plant growth for ecological and broader plant related applications.
authors.affiliation: School of Agriculture and Food Sciences, University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Jitka
authors.initials: J
authors.lastname: Kochanek
authors.affiliation: School of Chemistry and Biochemistry, University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Rowena L
authors.initials: RL
authors.lastname: Long
authors.affiliation: School of Agriculture and Food Sciences, University of Queensland, Gatton, Queensland, Australia.
authors.forename: Allan T
authors.initials: AT
authors.lastname: Lisle
authors.affiliation: School of Chemistry and Biochemistry, University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Gavin R
authors.initials: GR
authors.lastname: Flematti
mesh_terms: D044822:Biodiversity; D001937:Brassica / Q000254:growth & development; D002606:Charcoal / Q000032:analysis*; D004190:Disasters; D005390:Fires; D005663:Furans / Q000032:analysis*; D018525:Germination; D018545:Lactuca / Q000254:growth & development; D018551:Solanum lycopersicum / Q000254:growth & development; D011714:Pyrans / Q000032:analysis*; D036226:Seedlings / Q000254:growth & development*; D012639:Seeds / Q000737:chemistry; D032322:Solanum / Q000254:growth & development
chemical_list: D005663:Furans; D011714:Pyrans; C540010:biochar; C557869:karrikinolide; D002606:Charcoal