title: Transcriptomic changes in Nicotiana benthamiana plants inoculated with the wild-type or an attenuated mutant of Tobacco vein banding mosaic virus.
abstract: Tobacco vein banding mosaic virus (TVBMV) is a potyvirus which mainly infects solanaceous crops. The helper component proteinase (HCpro) of a potyvirus is an RNA silencing suppressor protein and determines the severity of disease symptoms caused by different potyviruses, including TVBMV. It has been shown that substitution mutations introduced into the HCpro open reading frame (ORF) in a TVBMV infectious clone result in changes of Asp189to Lys or Ile250-Gln251to Asp-Glu (Asp, aspartic acid; Gln, glutamine; Glu, glutamic acid; Ile, isoleucine). These amino acid changes eliminate the RNA silencing suppression activity of the mutant HCpro (HCm) and attenuate the disease symptoms caused by the mutant TVBMV (T-HCm) in Nicotiana benthamiana plants. Here, we used RNA-sequencing technology to compare gene expression in plants inoculated with the wild-type TVBMV (T-WT) with that in plants inoculated with T-HCm at 1, 2 and 10 days post-agroinfiltration (dpai). At 1 and 2 dpai, N. benthamiana genes related to the translation machinery were up-regulated, whereas genes related to lipid biosynthesis and metabolism or to responses to extracellular/external stimuli were down-regulated in leaves inoculated with T-WT or T-HCm. At 10 dpai, T-WT infection repressed photosynthesis-related genes. T-WT and T-HCm infections differentially perturbed the genes involved in the RNA silencing pathway. The salicylic acid and ethylene signalling pathways were induced, but the jasmonic acid signalling pathway was repressed after T-WT infection. Infections of T-WT and T-HCm also differentially regulated the genes involved in auxin signalling transduction, which is known to associate with the stunting phenotypes caused by TVBMV. These results illustrate the dynamic nature of TVBMV infection in N. benthamiana at the transcriptomic level.
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, 271018, China.|Key Laboratory for Agricultural Microbiology, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Geng
authors.affiliation: Key Laboratory for Agricultural Microbiology, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, 271018, China.|Key Laboratory for Agricultural Microbiology, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Yan
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, 271018, China.|Key Laboratory for Agricultural Microbiology, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Tian
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, 271018, China.|Key Laboratory for Agricultural Microbiology, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Yuan
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Gao
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, 271018, China.|Key Laboratory for Agricultural Microbiology, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Li
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D009154:Mutation / Q000235:genetics*; D010788:Photosynthesis / Q000235:genetics; D010937:Plant Growth Regulators / Q000378:metabolism; D017800:Potyvirus / Q000472:pathogenicity*; D034622:RNA Interference; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA; D015398:Signal Transduction; D014026:Nicotiana / Q000235:genetics* / Q000821:virology*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D010937:Plant Growth Regulators
keywords: Potyvirus; RNA-seq; Tobacco vein banding mosaic virus; attenuated mutant virus; transcriptomics