title: Evaluation of library preparation methods for Illumina next generation sequencing of small amounts of DNA from foodborne parasites.
abstract: Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as examples. The Ovation Ultralow method resulted in libraries with the highest concentration and produced quality sequencing data, even when the input DNA was in the picogram range.
authors.affiliation: Parasitic Disease Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30329, United States. Electronic address: yeb7@cdc.gov.
authors.forename: Fernanda S
authors.initials: FS
authors.lastname: Nascimento
authors.affiliation: Parasitic Disease Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30329, United States; IHRC Inc, 2 Ravinia Drive, Suite 1750, Atlanta, GA 30346, United States. Electronic address: saaspservices@gmail.com.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wei-Pridgeon
authors.affiliation: Waterborne Disease Prevention Branch, Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Enteric and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA, 30329, United States. Electronic address: mja0@cdc.gov.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Arrowood
authors.affiliation: Waterborne Disease Prevention Branch, Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Enteric and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA, 30329, United States. Electronic address: dmm3@cdc.gov.
authors.forename: Delynn
authors.initials: D
authors.lastname: Moss
authors.affiliation: Parasitic Disease Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30329, United States. Electronic address: Alexandre.DaSilva@fda.hhs.gov.
authors.forename: Alexandre J
authors.initials: AJ
authors.lastname: da Silva
authors.affiliation: Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30329, United States; Atlanta Research and Education Foundation, 1670 Clairmont Road, Suite 5A104, Decatur, GA 30033, United States.. Electronic address: wrj5@cdc.gov.
authors.forename: Eldin
authors.initials: E
authors.lastname: Talundzic
authors.affiliation: Parasitic Disease Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30329, United States. Electronic address: bvp2@cdc.gov.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Qvarnstrom
mesh_terms: D017185:Angiostrongylus cantonensis / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D021744:Cyclospora / Q000235:genetics; D016054:DNA, Protozoan / Q000032:analysis*; D016266:Food Parasitology; D005517:Foodborne Diseases / Q000469:parasitology*; D015723:Gene Library; D017125:Genes, Protozoan; D018503:Genome, Protozoan; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D008954:Models, Biological; D010271:Parasites / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan
keywords: Angiostrongylus cantonensis; Cyclospora cayetanensis; Illumina; Library preparation; MiSeq; Next generation sequencing