title: A novel recombinant Meq protein based dot-ELISA for rapid and confirmatory diagnosis of Marek's disease induced lymphoma in poultry.
abstract: Marek's disease (MD), is an economically important virus disease of poultry throughout the world. In this study, we for the first time reports development of a novel dot enzyme-linked immunosorbent assay (dot-ELISA) for the confirmatory diagnosis of lymphoma caused by Marek's Disease Virus (MDV). Suspected lymphoma tissue extracts from the diseased birds were used for the Meq oncoprotein antigen detection, which is expressed specifically in MDV lymphomas. Recombinant Meq oncoprotein was expressed using Expresso™ Rhamnose Sumo Cloning and Expression system and the hyperimmune serum was raised against it, which was used later while developing dot-ELISA. The dot-ELISA exhibited higher specificity (92%) in diagnosing MD lymphomas as compared to conventional PCR (40%), where later assay is unable to differentiate disease development (lymphoma) and/or infection. The developed dot-ELISA proved to be a specific, rapid and inexpensive technique detecting MDV lymphomas in poultry. Of the note, this new assay could be opted as a valuable diagnostic tool in the resource poor countries andcould further be used to differentiate from other tumor causing viruses in poultry.
authors.affiliation: Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P., India. Electronic address: drasokvet@gmail.com.
authors.forename: M Asok
authors.initials: MA
authors.lastname: Kumar
authors.affiliation: Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P., India. Electronic address: barathdassss@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Barathidasan
authors.affiliation: Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P., India. Electronic address: drpalvet@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Palanivelu
authors.affiliation: Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P., India. Electronic address: sdsingh2005@gmail.com.
authors.forename: S D
authors.initials: SD
authors.lastname: Singh
authors.affiliation: Immunology section, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P., India. Electronic address: mywani27@gmail.com.
authors.forename: Mohd Yaqoob
authors.initials: MY
authors.lastname: Wani
authors.affiliation: Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P., India. Electronic address: malikyps@ivri.res.in.
authors.forename: Yashpal Singh
authors.initials: YS
authors.lastname: Malik
authors.affiliation: Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P., India. Electronic address: rajendra_singh5747@rediffmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Singh
authors.affiliation: Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, U.P., India. Electronic address: kdhama@rediffmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Dhama
mesh_terms: D000818:Animals; D003365:Costs and Cost Analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008223:Lymphoma / Q000209:etiology*; D008380:Marek Disease / Q000150:complications / Q000175:diagnosis*; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D011201:Poultry Diseases / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: C100369:Eco-Q protein, Gallid herpesvirus 2; D009856:Oncogene Proteins, Viral
keywords: Diagnosis; Dot-ELISA; Lymphoma; Marek’s disease virus; Meq oncoprotein; Poultry