title: Characterization of the Far Transcription Factor Family in Aspergillus flavus.
abstract: Metabolism of fatty acids is a critical requirement for the pathogenesis of oil seed pathogens including the fungus Aspergillus flavus Previous studies have correlated decreased ability to grow on fatty acids with reduced virulence of this fungus on host seed. Two fatty acid metabolism regulatory transcription factors, FarA and FarB, have been described in other filamentous fungi. Unexpectedly, we find A. flavus possesses three Far homologs, FarA, FarB, and FarC, with FarA and FarC showing a greater protein similarity to each other than FarB. farA and farB are located in regions of colinearity in all Aspergillus spp. sequenced to date, whereas farC is limited to a subset of species where it is inserted in an otherwise colinear region in Aspergillus genomes. Deletion and overexpression (OE) of farA and farB, but not farC, yielded mutants with aberrant growth patterns on specific fatty acids as well as altered expression of genes involved in fatty acid metabolism. Marked differences included significant growth defects of both ∆farA and ∆farB on medium-chain fatty acids and decreased growth of OE::farA on unsaturated fatty acids. Loss of farA diminished expression of mitochondrial β-oxidation genes whereas OE::farA inhibited expression of genes involved in unsaturated fatty acid catabolism. FarA also positively regulated the desaturase genes required to generate polyunsaturated fatty acids. Aflatoxin production on toxin-inducing media was significantly decreased in the ∆farB mutant and increased in the OE::farB mutant, with gene expression data supporting a role for FarB in tying β-oxidation processes with aflatoxin accumulation.
authors.affiliation: Department of Plant Pathology, University of Wisconsin-Madison, Wisconsin 53706.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Wisconsin 53706.
authors.forename: Katharyn J
authors.initials: KJ
authors.lastname: Affeldt
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Wisconsin 53706 Department of Bacteriology, University of Wisconsin-Madison, Wisconsin 53706 npkeller@wisc.edu.
authors.forename: Nancy P
authors.identifier: 0000-0002-4386-9473
authors.initials: NP
authors.lastname: Keller
mesh_terms: D000348:Aflatoxins / Q000378:metabolism; D001231:Aspergillus flavus / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity; D005227:Fatty Acids / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D015870:Gene Expression; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D050356:Lipid Metabolism; D005810:Multigene Family; D009154:Mutation; D010084:Oxidation-Reduction; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D059467:Transcriptome; D014774:Virulence; D003313:Zea mays / Q000382:microbiology
chemical_list: D000348:Aflatoxins; D005227:Fatty Acids; D005656:Fungal Proteins; D014157:Transcription Factors
keywords: FlyBook; aflatoxin; fatty acid metabolism; gene family; virulence; β-oxidation