title: Molecular characterization and expression analysis of Hsp90 in Schizothorax prenanti.
abstract: Aquatic animals suffer from various environmental stresses because the aquatic environment is a very complex system. To monitor the health status of fish, Hsp90 a potential early warning marker was determined in Schizothorax prenanti after infection with a bacterium. In this study, we cloned Hsp90 from S. prenanti for the first time. The full-length cDNA sequence of SpHsp90 was 2663 bp, contains an open reading frame of 2181 bp, and has a gene encoding 726 amino acids, an estimated molecular mass of 83.38 kDa, and a theoretical isoelectric point of 4.91. The SpHsp90 amino acid sequence has five conserved HSP90 family signatures and shares 87.0-95.5 % identity with other vertebrates. Phylogenetic analysis and structure comparison indicated that SpHsp90 should be a β isoform of the HSP90 family. SpHsp90 was ubiquitously expressed in all examined tissues, and the highest level of expression was in the kidney. After Streptococcus agalactiae infection, the level of SpHsp90 expression had significant changes (P < 0.05) in the hepatopancreas, spleen, kidney, and blood. The expression increased to the highest level at 6 h in the blood and at 24 h in the hepatopancreas, spleen, and kidney. The results suggested that the SpHsp90 gene could be induced by S. agalactiae in S. prenanti and that SpHsp90 may be involved in resistance to bacterial infection and provide an early warning information. The kidney is the most suitable for detecting SpHsp90 after bacterial infection.
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, WenJiang District Huimin Road No. 211, ChengDu, Sichuan Province, 611130, China.
authors.forename: Yundan
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, WenJiang District Huimin Road No. 211, ChengDu, Sichuan Province, 611130, China.
authors.forename: Jieyao
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, WenJiang District Huimin Road No. 211, ChengDu, Sichuan Province, 611130, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, WenJiang District Huimin Road No. 211, ChengDu, Sichuan Province, 611130, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, WenJiang District Huimin Road No. 211, ChengDu, Sichuan Province, 611130, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hao
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, WenJiang District Huimin Road No. 211, ChengDu, Sichuan Province, 611130, China.
authors.forename: Yuanbin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, WenJiang District Huimin Road No. 211, ChengDu, Sichuan Province, 611130, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Geng
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, WenJiang District Huimin Road No. 211, ChengDu, Sichuan Province, 611130, China.
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, WenJiang District Huimin Road No. 211, ChengDu, Sichuan Province, 611130, China.
authors.forename: Zhiqiong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Fisheries Research Institute, Sichuan Academy of Agricultural Sciences, ChengDu, China. zhoujian980@126.com.|Sichuan Agricultural University, WenJiang District Huimin Road No. 211, ChengDu, China. zhoujian980@126.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, WenJiang District Huimin Road No. 211, ChengDu, Sichuan Province, 611130, China. chendf_sicau@126.com.|Fisheries Research Institute, Sichuan Academy of Agricultural Sciences, ChengDu, China. chendf_sicau@126.com.|Sichuan Agricultural University, WenJiang District Huimin Road No. 211, ChengDu, China. chendf_sicau@126.com.
authors.forename: Defang
authors.initials: D
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D007526:Isoelectric Point; D008970:Molecular Weight; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D018841:HSP90 Heat-Shock Proteins; D012313:RNA
keywords: Bacterial infection; Gene expression; Hsp90; Molecular characterization; Schizothorax prenanti