title: A Microfluidic Approach for Inducing Cell Rotation by Means of Hydrodynamic Forces.
abstract: Microfluidic technology allows to realize devices in which cells can be imaged in their three-dimensional shape. However, there are still some limitations in the method, due to the fact that cells follow a straight path while they are flowing in a channel. This can result in a loss in information, since only one side of the cell will be visible. Our work has started from the consideration that if a cell rotates, it is possible to overcome this problem. Several approaches have been proposed for cell manipulation in microfluidics. In our approach, cells are controlled by only taking advantages of hydrodynamic forces. Two different devices have been designed, realized, and tested. The first device induces cell rotation in a plane that is parallel (in-plane) to the observation plane, while the second one induce rotation in a plane perpendicular (out-of-plane) to the observation plane.
authors.affiliation: Institute for Microelectronics and Microsystems, National Research Council, Naples 80131, Italy. stefania.torino@na.imm.cnr.it.|Rowland Institute at Harvard, Harvard University, 100 E. Land Blvd., Cambridge, MA 02142, USA. stefania.torino@na.imm.cnr.it.
authors.forename: Stefania
authors.initials: S
authors.lastname: Torino
authors.affiliation: Institute for Microelectronics and Microsystems, National Research Council, Naples 80131, Italy. mario.iodice@cnr.it.
authors.forename: Mario
authors.initials: M
authors.lastname: Iodice
authors.affiliation: Institute for Microelectronics and Microsystems, National Research Council, Naples 80131, Italy. ivo.rendina@cnr.it.
authors.forename: Ivo
authors.initials: I
authors.lastname: Rendina
authors.affiliation: Institute for Microelectronics and Microsystems, National Research Council, Naples 80131, Italy. giuseppe.coppola@cnr.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Coppola
authors.affiliation: Rowland Institute at Harvard, Harvard University, 100 E. Land Blvd., Cambridge, MA 02142, USA. schonbrun@rowland.harvard.edu.
authors.forename: Ethan
authors.initials: E
authors.lastname: Schonbrun
keywords: hydrodynamic manipulation; microfluidics; rotation