title: Fucan-coated silver nanoparticles synthesized by a green method induce human renal adenocarcinoma cell death.
abstract: Polysaccharides containing sulfated L-fucose are often called fucans. The seaweed Spatoglossum schröederi synthesizes three fucans, among which fucan A is the most abundant. This polymer is not cytotoxic against various normal cell lines and is non-toxic to rats when administered at high doses. In addition, it exhibits low toxicity against tumor cells. With the aim of increasing the toxicity of fucan A, silver nanoparticles containing this polysaccharide were synthesized using a green chemistry method. The mean size of these nanoparticles was 210nm. They exhibited a spherical shape and negative surface charge and were stable for 14 months. When incubated with cells, these nanoparticles did not show any toxic effects against various normal cell lines; however, they decreased the viability of various tumor cells, especially renal adenocarcinoma cells 786-0. Flow cytometry analyses showed that the nanoparticles induced cell death responses of 786-0 cells through necrosis. Assays performed with several renal cell lines (HEK, VERO, MDCK) showed that these nanoparticles only induce death of 786-0 cells. The data obtained herein leads to the conclusion that fucan A nanoparticles are promising agents against renal adenocarcinoma.
authors.affiliation: Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, Brazil; Programa de Pós-graduação em Ciências da Saúde, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte - RN 59078-970, Brazil.
authors.forename: Monica Oliveira
authors.initials: MO
authors.lastname: Rocha Amorim
authors.affiliation: Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, Brazil; Programa de Pós-graduação em Ciências da Saúde, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte - RN 59078-970, Brazil.
authors.forename: Dayanne
authors.initials: D
authors.lastname: Lopes Gomes
authors.affiliation: Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, Brazil.
authors.forename: Larisse Araujo
authors.initials: LA
authors.lastname: Dantas
authors.affiliation: Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, Brazil.
authors.forename: Rony Lucas
authors.initials: RL
authors.lastname: Silva Viana
authors.affiliation: Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, Brazil.
authors.forename: Samanta Cristina
authors.initials: SC
authors.lastname: Chiquetti
authors.affiliation: Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, Brazil.
authors.forename: Jailma
authors.initials: J
authors.lastname: Almeida-Lima
authors.affiliation: Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, Brazil; Intituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte (IFRN), Ceara-Mirim, Rio Grande do Norte - RN, 59580-000, Brazil.
authors.forename: Leandro
authors.initials: L
authors.lastname: Silva Costa
authors.affiliation: Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte- RN 59078-970, Brazil; Programa de Pós-graduação em Ciências da Saúde, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte - RN 59078-970, Brazil. Electronic address: hugo-alexandre@uol.com.br.
authors.forename: Hugo Alexandre
authors.initials: HA
authors.lastname: Oliveira Rocha
mesh_terms: D016475:3T3 Cells; D000230:Adenocarcinoma / Q000188:drug therapy; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005643:Fucose / Q000737:chemistry; D055772:Green Chemistry Technology; D057809:HEK293 Cells; D006801:Humans; D007680:Kidney Neoplasms / Q000188:drug therapy; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry; D012834:Silver / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D005643:Fucose; D012834:Silver
keywords: Fucoidan; Necrosis; Renal adenocarcinoma; Spatoglossum schröederi