title: Efficacy of platelet-rich plasma as a shielding technique after endoscopic mucosal resection in rat and porcine models.
abstract: OBJECTIVE
  The aims were to assess the efficacy of endoscopic application of Platelet-rich plasma (PRP) to prevent delayed perforation and to induce mucosal healing after endoscopic resections.
  
  
  METHODS
  Colonic induced lesions were performed in rats (n = 16) and pigs (n = 4). Animals were randomized to receive onto the lesions saline (control) or PRP. Animals underwent endoscopic follow-up. Thermal injury was assessed with a 1 - 4 scale: (1) mucosal necrosis; (2) submucosal necrosis; (3) muscularis propria necrosis; and (4) serosal necrosis
  
  
  RESULTS
  Saline treatment showed 50 % of mortality in rats (P = 0.02). Mean ulcerated area after 48 hours and 7 days was significantly smaller with PRP than with saline (0.27 ± 0.02 cm(2) and 0.08 ± 0.01 cm(2) vs. 0.56 ± 0.1 cm(2) and 0.40 ± 0.06 cm(2); P < 0.001). The incidence of thermal injury was significantly lower with PRP (1.25 ± 0.46) than in controls (2.25 ± 0.50); P = 0.006. The porcine model showed a trend toward higher mucosal restoration in animals treated with PRP than with saline at weeks 1 and 2 (Median area in cm(2): 0.55 and 0.40 vs. 1.32 and 0.79) CONCLUSIONS: Application of PRP to colonic mucosal lesions showed strong healing properties in rat and porcine models.
authors.affiliation: Endoscopy Unit, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Barcelona, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Lorenzo-Zúñiga
authors.affiliation: Endoscopy Unit, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Barcelona, Spain.
authors.forename: Jaume
authors.initials: J
authors.lastname: Boix
authors.affiliation: Endoscopy Unit, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Barcelona, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Moreno de Vega
authors.affiliation: Endoscopy Unit, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Bon
authors.affiliation: Endoscopy Unit, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Marín
authors.affiliation: Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Barcelona, Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: Bartolí