title: Characterization of a highly toxic strain of Bacillus thuringiensis serovar kurstaki very similar to the HD-73 strain.
abstract: The LBIT-1200 strain of Bacillus thuringiensis was recently isolated from soil, and showed a 6.4 and 9.5 increase in toxicity, against Manduca sexta and Trichoplusia ni, respectively, compared to HD-73. However, LBIT-1200 was still highly similar to HD-73, including the production of bipyramidal crystals containing only one protein of ∼130 000 kDa, its flagellin gene sequence related to the kurstaki serotype, plasmid and RepPCR patterns similar to HD-73, no production of β-exotoxin and no presence of VIP genes. Sequencing of its cry gene showed the presence of a cry1Ac-type gene with four amino acid differences, including two amino acid replacements in domain III, compared to Cry1Ac1, which may explain its higher toxicity. In conclusion, the LBIT-1200 strain is a variant of the HD-73 strain but shows a much higher toxicity, which makes this new strain an important candidate to be developed as a bioinsecticide, once it passes other tests, throughout its biotechnological development.
authors.affiliation: Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, 36500 Irapuato, Guanajuato, Mexico.
authors.forename: Yaritza
authors.initials: Y
authors.lastname: Reinoso-Pozo
authors.affiliation: Departamento de Alimentos, División de Ciencias de la Vida, Universidad de Guanajuato, 36500 Irapuato, Guanajuato, Mexico.
authors.forename: Ma Cristina
authors.initials: MC
authors.lastname: Del Rincón-Castro
authors.affiliation: Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, 36500 Irapuato, Guanajuato, Mexico jibarra@ira.cinvestav.mx.
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Ibarra
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D001426:Bacterial Proteins / Q000235:genetics*; D004731:Endotoxins / Q000235:genetics; D005098:Exotoxins / Q000378:metabolism; D005408:Flagellin / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D007306:Insecticides; D009036:Moths; D010572:Pest Control, Biological; D010957:Plasmids; D017422:Sequence Analysis, DNA; D065288:Serogroup; D018411:Spodoptera
chemical_list: D001426:Bacterial Proteins; D004731:Endotoxins; D005098:Exotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; D005408:Flagellin
keywords: cry gene; kurstaki; new isolate; thuringiensis; toxicity