title: A Framework for Discovering, Designing, and Testing MicroProteins to Regulate Synthetic Transcriptional Modules.
abstract: Transcription factors often form protein complexes and give rise to intricate transcriptional networks. The regulation of transcription factor multimerization plays a key role in the fine-tuning of the underlying transcriptional pathways and can be exploited to modulate synthetic transcriptional modules. A novel regulation of protein complex formation is emerging: microProteins-truncated transcription factors-engage in protein-protein interactions with transcriptional complexes and modulate their transcriptional activity. Here, we outline a strategy for the discovery, design, and test of putative miPs to fine-tune the activity of transcription factors regulating synthetic or natural transcriptional circuits.
authors.affiliation: Institut Jean-Pierre Bourgin, INRA Centre de Versailles-Grignon, Bâtiment 2, Route de St-Cyr (RD10), 78026, Versailles Cedex, France.
authors.forename: Elisa
authors.initials: E
authors.lastname: Fiume
authors.affiliation: Department of Plant Biology, Carnegie Institution for Science, 260 Panama Street, Stanford, CA, 94305, USA.|Department of Genetics, CB50, University Medical Center Groningen, 30001, 9700, RB, Groningen, The Netherlands.
authors.forename: Niek
authors.initials: N
authors.lastname: de Klein
authors.affiliation: Department of Plant Biology, Carnegie Institution for Science, 260 Panama Street, Stanford, CA, 94305, USA.
authors.forename: Seung Yon
authors.initials: SY
authors.lastname: Rhee
authors.affiliation: Institut Jean-Pierre Bourgin, INRA Centre de Versailles-Grignon, Bâtiment 2, Route de St-Cyr (RD10), 78026, Versailles Cedex, France. enricomagnani@versailles.inra.fr.
authors.forename: Enrico
authors.initials: E
authors.lastname: Magnani
mesh_terms: D005786:Gene Expression Regulation / Q000235:genetics*; D053263:Gene Regulatory Networks / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D060066:Protein Interaction Maps / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D011506:Proteins; D014157:Transcription Factors
keywords: Multimerization; Protein complex; Protein–protein interaction domain; Synthetic promoter; Transcription factor; microProteins