title: Transposon-Sequencing Analysis Unveils Novel Genes Involved in the Generation of Persister Cells in Uropathogenic Escherichia coli.
abstract: Persister cells are highly tolerant to different antibiotics and are associated with relapsing infections. In order to understand this phenomenon further, we exposed a transposon library to a lethal concentration of ampicillin, and mutants that survived were identified by transposon sequencing (Tn-Seq). We determined that mutations related to carbon metabolism, cell envelope (cell wall generation and membrane proteins), and stress response have a role in persister cell generation.
authors.affiliation: Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine, Boston, Massachusetts, USA.|Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.
authors.forename: Roberto C
authors.initials: RC
authors.lastname: Molina-Quiroz
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.
authors.forename: David W
authors.initials: DW
authors.lastname: Lazinski
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.|Howard Hughes Medical Institute, Boston, Massachusetts, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Camilli
authors.affiliation: Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine, Boston, Massachusetts, USA stuart.levy@tufts.edu.|Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.
authors.forename: Stuart B
authors.initials: SB
authors.lastname: Levy
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004251:DNA Transposable Elements / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009154:Mutation; D056689:Uropathogenic Escherichia coli / Q000187:drug effects* / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D000667:Ampicillin