title: A thioacidolysis method tailored for higher-throughput quantitative analysis of lignin monomers.
abstract: Thioacidolysis is a method used to measure the relative content of lignin monomers bound by β-O-4 linkages. Current thioacidolysis methods are low-throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis method that is accessible with general laboratory equipment and uses a non-chlorinated organic solvent and is tailored for higher-throughput analysis is reported. The method utilizes lignin arylglycerol monomer standards for calibration, requires 1-2 mg of biomass per assay and has been quantified using fast-GC techniques including a Low Thermal Mass Modular Accelerated Column Heater (LTM MACH). Cumbersome steps, including standard purification, sample concentrating and drying have been eliminated to help aid in consecutive day-to-day analyses needed to sustain a high sample throughput for large screening experiments without the loss of quantitation accuracy. The method reported in this manuscript has been quantitatively validated against a commonly used thioacidolysis method and across two different research sites with three common biomass varieties to represent hardwoods, softwoods, and grasses.
authors.affiliation: Bioenergy Science Center, Golden, CO, USA. Anne.Ware@nrel.gov.|National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, USA. Anne.Ware@nrel.gov.
authors.forename: Anne E
authors.initials: AE
authors.lastname: Harman-Ware
authors.affiliation: Great Lakes BioEnergy Research Center, Michigan State University, East Lansing, MI, USA.
authors.forename: Cliff
authors.initials: C
authors.lastname: Foster
authors.affiliation: Bioenergy Science Center, Golden, CO, USA.|National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, USA.
authors.forename: Renee M
authors.initials: RM
authors.lastname: Happs
authors.affiliation: Bioenergy Science Center, Golden, CO, USA.|National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, USA.
authors.forename: Crissa
authors.initials: C
authors.lastname: Doeppke
authors.affiliation: Great Lakes BioEnergy Research Center, Michigan State University, East Lansing, MI, USA.
authors.forename: Kristoffer
authors.initials: K
authors.lastname: Meunier
authors.affiliation: Great Lakes BioEnergy Research Center, Michigan State University, East Lansing, MI, USA.
authors.forename: Jackson
authors.initials: J
authors.lastname: Gehan
authors.affiliation: Wisconsin Bioenergy Initiative, University of Wisconsin, Madison, WI, USA.
authors.forename: Fengxia
authors.initials: F
authors.lastname: Yue
authors.affiliation: Wisconsin Bioenergy Initiative, University of Wisconsin, Madison, WI, USA.
authors.forename: Fachuang
authors.initials: F
authors.lastname: Lu
authors.affiliation: Bioenergy Science Center, Golden, CO, USA.|National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, USA.
authors.forename: Mark F
authors.initials: MF
authors.lastname: Davis
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005990:Glycerol / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000379:methods*; D008031:Lignin / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006109:Poaceae / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D014934:Wood / Q000737:chemistry
chemical_list: D013438:Sulfhydryl Compounds; D008031:Lignin; D005990:Glycerol
keywords: Cell wall structure; Lignin; S/G ratio; Thioacidolysis