title: Artemisinic Acid Serves as a Novel ORCA3 Inducer to Enhance Biosynthesis of Terpenoid Indole Alkaloids in Catharanthus roseus Cambial Meristematic Cells.
abstract: To investigate the effect of artemisinic acid (AA) on improving the production of terpenoid indole alkaloids (TIAs) of Catharanthus roseus cambial meristematic cells (CMCs), feeding AA to C. roseus CMCs caused 2.35-fold and 2.51-fold increases in the production of vindoline and catharanthine, respectively, compared with those of the untreated CMCs. qRT-PCR experiments showed that AA resulted in a 1.36-8.52 fold increase in the transcript levels of several related genes, including octadecanoid-derivative responsive Catharanthus AP2-domain protein 3 (ORCA3), tryptophan decarboxylase (TDC), strictosidine synthase (STR) and desacetoxyvindoline 4-hydroxylase (D4H). However, no effect was observed on the concentration of either jasmonic acid (JA), or the octadecanoid-pathway inhibitors block TIA accumulation caused by AA. The results indicated that AA might serve as a novel ORCA3 inducer to manipulate biosynthesis of TIAs in C. roseus CMCs via an unknown mechanism.
authors.forename: Mingxuan
authors.initials: M
authors.lastname: Wang
authors.forename: Jiachen
authors.initials: J
authors.lastname: Zi
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
authors.forename: Shan
authors.initials: S
authors.lastname: Chen
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.forename: Liyan
authors.initials: L
authors.lastname: Song
authors.forename: Rongmin
authors.initials: R
authors.lastname: Yu
mesh_terms: D037621:Artemisinins / Q000494:pharmacology*; D030008:Catharanthus / Q000187:drug effects / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018519:Meristem / Q000187:drug effects / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D046948:Secologanin Tryptamine Alkaloids / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D037621:Artemisinins; C410983:ORCA3 protein, plant; D010940:Plant Proteins; D046948:Secologanin Tryptamine Alkaloids; D014157:Transcription Factors; C047721:artemisic acid