title: Screening, identification, and characterization of a novel saccharide-stimulated β-glycosidase from a soil metagenomic library.
abstract: MeBglD2, a β-glycosidase that is highly activated in the presence of various monosaccharides and disaccharides, was isolated from a soil metagenomic library. MeBglD2 had not only β-glucosidase activity but also β-galactosidase and β-fucosidase activities. MeBglD2 β-glucosidase activity increased in a cellobiose concentration-dependent manner and was not inhibited by a high concentration of D-glucose or cellobiose. MeBglD2 β-glucosidase and β-fucosidase activities were activated by various monosaccharides and disaccharides including D-glucose, D-xylose, D-galactose, maltose, and cellobiose. The saccharification yield of rice straw using Trichoderma reesei cellulase was improved by the addition of MeBglD2. These results show that MeBglD2 can be used to improve plant biomass saccharification, because both substrates and products can activate its enzymatic activity.
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan. matsuzawa-tomohiko@aist.go.jp.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Matsuzawa
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan. k-yaoi@aist.go.jp.
authors.forename: Katsuro
authors.initials: K
authors.lastname: Yaoi
mesh_terms: D001711:Biotransformation; D002480:Cellulase / Q000378:metabolism; D016000:Cluster Analysis; D004187:Disaccharides / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects*; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D054892:Metagenome; D009005:Monosaccharides / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism; D010802:Phylogeny; D018547:Plant Stems / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012987:Soil; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology
chemical_list: D004187:Disaccharides; D009005:Monosaccharides; D012987:Soil; D006026:Glycoside Hydrolases; D002480:Cellulase
keywords: Biomass; Glycoside hydrolase family 1; Metagenome; β-Glycosidase