title: Tailored parameter optimization methods for ordinary differential equation models with steady-state constraints.
abstract: Ordinary differential equation (ODE) models are widely used to describe (bio-)chemical and biological processes. To enhance the predictive power of these models, their unknown parameters are estimated from experimental data. These experimental data are mostly collected in perturbation experiments, in which the processes are pushed out of steady state by applying a stimulus. The information that the initial condition is a steady state of the unperturbed process provides valuable information, as it restricts the dynamics of the process and thereby the parameters. However, implementing steady-state constraints in the optimization often results in convergence problems.
  
  
  
  In this manuscript, we propose two new methods for solving optimization problems with steady-state constraints. The first method exploits ideas from optimization algorithms on manifolds and introduces a retraction operator, essentially reducing the dimension of the optimization problem. The second method is based on the continuous analogue of the optimization problem. This continuous analogue is an ODE whose equilibrium points are the optima of the constrained optimization problem. This equivalence enables the use of adaptive numerical methods for solving optimization problems with steady-state constraints. Both methods are tailored to the problem structure and exploit the local geometry of the steady-state manifold and its stability properties. A parameterization of the steady-state manifold is not required. The efficiency and reliability of the proposed methods is evaluated using one toy example and two applications. The first application example uses published data while the second uses a novel dataset for Raf/MEK/ERK signaling. The proposed methods demonstrated better convergence properties than state-of-the-art methods employed in systems and computational biology. Furthermore, the average computation time per converged start is significantly lower. In addition to the theoretical results, the analysis of the dataset for Raf/MEK/ERK signaling provides novel biological insights regarding the existence of feedback regulation.
  
  
  
  Many optimization problems considered in systems and computational biology are subject to steady-state constraints. While most optimization methods have convergence problems if these steady-state constraints are highly nonlinear, the methods presented recover the convergence properties of optimizers which can exploit an analytical expression for the parameter-dependent steady state. This renders them an excellent alternative to methods which are currently employed in systems and computational biology.
authors.affiliation: Institute of Computational Biology, Helmholtz Zentrum München, Ingolstädter Landstraße 1, Neuherberg, 85764, Germany.|Chair of Mathematical Modeling of Biological Systems, Center for Mathematics, Technische Universität München, Boltzmannstraße 3, Garching, 85748, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Fiedler
authors.affiliation: Stuttgart Research Center Systems Biology (SRCSB), University of Stuttgart, Nobelstr. 15, Stuttgart, 70569, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Raeth
authors.affiliation: Institute of Computational Biology, Helmholtz Zentrum München, Ingolstädter Landstraße 1, Neuherberg, 85764, Germany.|Chair of Mathematical Modeling of Biological Systems, Center for Mathematics, Technische Universität München, Boltzmannstraße 3, Garching, 85748, Germany.
authors.forename: Fabian J
authors.initials: FJ
authors.lastname: Theis
authors.affiliation: Stuttgart Research Center Systems Biology (SRCSB), University of Stuttgart, Nobelstr. 15, Stuttgart, 70569, Germany.
authors.forename: Angelika
authors.initials: A
authors.lastname: Hausser
authors.affiliation: Institute of Computational Biology, Helmholtz Zentrum München, Ingolstädter Landstraße 1, Neuherberg, 85764, Germany. jan.hasenauer@helmholtz-muenchen.de.|Chair of Mathematical Modeling of Biological Systems, Center for Mathematics, Technische Universität München, Boltzmannstraße 3, Garching, 85748, Germany. jan.hasenauer@helmholtz-muenchen.de.
authors.forename: Jan
authors.initials: J
authors.lastname: Hasenauer
mesh_terms: D000465:Algorithms; D019295:Computational Biology / Q000379:methods*; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D025461:Feedback, Physiological / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008954:Models, Biological; D020932:Nerve Growth Factor / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D015203:Reproducibility of Results; D059807:S Phase Cell Cycle Checkpoints / Q000187:drug effects; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000378:metabolism
chemical_list: D020932:Nerve Growth Factor; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Differential equation; Parameter optimization; Perturbation experiments; Steady state