title: Probiotic Potential of Autochthonous Bacteria Isolated from the Gastrointestinal Tract of Four Freshwater Teleosts.
abstract: In this study, a total of 121 bacterial strains were isolated from the gastrointestinal tract of four teleostean species, namely striped snakehead (Channa striatus), striped dwarf catfish (Mystus vittatus), orangefin labeo (Labeo calbasu) and mrigal carp (Cirrhinus mrigala), among which 8 isolates showed promising antibacterial activity against four potential fish pathogens, Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas sobria and Pseudomonas fluorescens and were non-hemolytic. The isolates were further screened in response to fish bile tolerance and extracellular digestive enzyme activity. Two bacterial strains MVF1 and MVH7 showed highest tolerance and extracellular enzymes activities, and selected for further studies. Antagonistic activity of these two isolates was further confirmed by in vitro growth inhibition assay against four selected fish pathogens in liquid medium. Finally, these two bacterial strains MVF1 and MVH7 were selected as potential probiotic candidates and thus identification by partial 16S rRNA gene sequence analysis. The bacterial isolates MVF1 and MVH7 were identified as two strains of Bacillus sp.
authors.affiliation: Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan, West Bengal, 731235, India.
authors.forename: Ankita
authors.initials: A
authors.lastname: Nandi
authors.affiliation: Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan, West Bengal, 731235, India.
authors.forename: Suhas Kumar
authors.initials: SK
authors.lastname: Dan
authors.affiliation: Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan, West Bengal, 731235, India.
authors.forename: Goutam
authors.initials: G
authors.lastname: Banerjee
authors.affiliation: Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan, West Bengal, 731235, India.
authors.forename: Pinki
authors.initials: P
authors.lastname: Ghosh
authors.affiliation: Department of Zoology, University of Burdwan, Golap Bag, Burdwan, West Bengal, 713 104, India.
authors.forename: Koushik
authors.initials: K
authors.lastname: Ghosh
authors.affiliation: Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, UiT The Arctic University of Norway, 9037, Breivika, Norway.
authors.forename: Einar
authors.initials: E
authors.lastname: Ringø
authors.affiliation: Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan, West Bengal, 731235, India. aray51@yahoo.com.
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Ray
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D002347:Carps / Q000382:microbiology; D002397:Catfishes / Q000382:microbiology; D003530:Cyprinidae / Q000382:microbiology; D005399:Fishes / Q000382:microbiology*; D005618:Fresh Water / Q000032:analysis; D041981:Gastrointestinal Tract / Q000382:microbiology*; D019936:Probiotics / Q000145:classification / Q000302:isolation & purification*
keywords: Antagonism; Bacillus; Gut bacteria; Probiotics; Teleost