title: The anti-obesity effect of Taheebo (Tabebuia avellanedae Lorentz ex Griseb) extract in ovariectomized mice and the identification of a potential anti-obesity compound.
abstract: Estrogen deficiency-induced obesity has a high risk of visceral fat accumulation and body weight gain. It is also associated with many adverse health conditions. Taheebo extract from Tabebuia avellanedae has been recognized as playing several biological and pharmacological roles. Therefore, we investigated whether the intake of n-BuOH extract of Taheebo shows anti-obesity effect in ovariectomized (OVX) mice. After 16 weeks of feeding, the mice administrated with 0.5% n-BuOH extract of Taheebo showed significantly decreased body weight compared with that of the control mice, and the fat mass also showed a significant decrease. In 3T3-L1 cells, supplementation with n-BuOH extract of Taheebo significantly reduced the triglyceride (TG) levels. Furthermore, bioassay-guided purification of the n-BuOH extract based on the TG levels in 3T3-L1 cells led to the isolation of compound 2 (1-dehydroxy-3,4-dihydroaucubigenin). These results suggested that the anti-obesity effect of Taheebo extract is due to its capability in preventing the accumulation of adipocyte in mice. Taheebo extract might be a promising functional food resources capable of protecting against OVX-induced obesity.
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara City 631-8505, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Iwamoto
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara City 631-8505, Japan.
authors.forename: Youichi
authors.initials: Y
authors.lastname: Fukuda
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara City 631-8505, Japan.
authors.forename: Chisato
authors.initials: C
authors.lastname: Tokikura
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara City 631-8505, Japan.
authors.forename: Mayuko
authors.initials: M
authors.lastname: Noda
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara City 631-8505, Japan.
authors.forename: Ayami
authors.initials: A
authors.lastname: Yamamoto
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara City 631-8505, Japan.
authors.forename: Misato
authors.initials: M
authors.lastname: Yamamoto
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara City 631-8505, Japan.
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Yamashita
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara City 631-8505, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Zaima
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara City 631-8505, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Iida
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, 204-3327 Nakamachi, Nara City 631-8505, Japan. Electronic address: tmoriyama@nara.kindai.ac.jp.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Moriyama
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D041721:3T3-L1 Cells; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005243:Feces / Q000737:chemistry; D005260:Female; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009765:Obesity / Q000097:blood / Q000188:drug therapy*; D009929:Organ Size / Q000187:drug effects; D010052:Ovariectomy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D029663:Tabebuia / Q000737:chemistry*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D019440:Anti-Obesity Agents; D010936:Plant Extracts; C543691:Taheebo; D014280:Triglycerides; D020001:1-Butanol
keywords: 1-dehydroxy-3,4-dihydroaucubigenin; Anti-obesity; Ovariectomized mice; Tabebuia avellanedae Lorentz ex Griseb