title: Amphiphilic poly-N-vynilpyrrolidone nanoparticles: Cytotoxicity and acute toxicity study.
abstract: The aim of the present study was to evaluate the cytotoxicity against MCF-7 cells and acute intraperitoneal toxicity of amphiphilic poly-N-vinylpyrrolidone nanoparticles to confirm possibility of their application for creation of novel drug delivery systems. The effect of cellular uptake of polymeric nanoparticles on human cancer cell line MCF-7 cells was investigated by MTT assay. MTT analysis showed that tested amphiphilic polymers were essentially non-toxic. In acute toxicity studies, LD50 and other toxicity indexes were evaluated, under which no deaths or treatment related complications were observed even in high concentration treatment for 14 days of experiment. For histological analysis, organs of the animals were weighed and examined. No animal died during the study and no significant changes have been observed regarding body weight, feed consumption, organ weight or histological data. Obtained results show that amphiphilic poly-N-vinylpyrrolidone nanoparticles possessed no toxicity against cells and in animals after intraperitoneal administration. Thus, amphiphilic PVP nanoparticles demonstrate high potential as carriers for novel high-effective drug delivery systems.
authors.affiliation: D.I. Mendeleyev University of Chemical Technology of Russia, Moscow 125047, Russian Federation; Moscow State University of Mechanical Engineering (MAMI), Moscow 107023, Russian Federation. Electronic address: a_n_kuskov@mail.ru.
authors.forename: A N
authors.initials: AN
authors.lastname: Kuskov
authors.affiliation: D.I. Mendeleyev University of Chemical Technology of Russia, Moscow 125047, Russian Federation.
authors.forename: P P
authors.initials: PP
authors.lastname: Kulikov
authors.affiliation: D.I. Mendeleyev University of Chemical Technology of Russia, Moscow 125047, Russian Federation.
authors.forename: M I
authors.initials: MI
authors.lastname: Shtilman
authors.affiliation: Federal Scientific Center of Hygiene, F.F. Erisman, Moscow 141014, Russian Federation.
authors.forename: V N
authors.initials: VN
authors.lastname: Rakitskii
authors.affiliation: University of Crete, Medical School, Laboratory of Toxicology, Voutes, Heraklion 71409, Crete, Greece.
authors.forename: A M
authors.initials: AM
authors.lastname: Tsatsakis
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000633:toxicity; D001835:Body Weight / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D061986:MCF-7 Cells; D008297:Male; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011760:Pyrrolidinones / Q000008:administration & dosage / Q000633:toxicity*; D023382:Toxicity Tests, Acute
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D011108:Polymers; D011760:Pyrrolidinones; C042670:N-vinyl-2-pyrrolidinone
keywords: Acute toxicity; Amphiphilic; Cytotoxicity; Drug delivery system; Nanoparticle; Poly-N-vinylpyrrolidone