title: Increased expression of microRNA-155 in peripheral blood mononuclear cells from psoriasis patients is related to disease activity.
abstract: BACKGROUND
  MicroRNAs (miRNAs) gene expression regulators are altered in psoriasis suggesting their role in the pathogenesis.
  
  
  OBJECTIVE
  To study expression changes of inflammation and toll-like receptor (TLR)-related miRNAs, miRNA-155, let-7i, miRNA-21, miRNA-146a and miRNA-223 in peripheral mononuclear cells (PBMCs) and miRNA-21, miRNA-146a and miRNA-223 in plasma, from chronic plaque-type psoriasis patients who were treatment-naive or had undergone a washout period (n = 11). MiRNAs were evaluated at baseline and after 11 (9-12) months [median (25th-75th percentile range)] of methotrexate (MTX) or topical (betamethasone plus calcipotriene) treatment.
  
  
  METHODS
  MiRNA expression was analysed with quantitative real-time reverse transcription-polymerase chain reaction. Matched controls were studied.
  
  
  RESULTS
  Psoriasis patients presented, at baseline, increased expression of miRNA-155, let-7i, miRNA-146a, miRNA-21 and miRNA-223 in PBMCs, plus miRNA-21, miRNA-146a and miRNA-223 in plasma. Receiver-operator characteristic (ROC) curve analysis and area under the curve (AUC) showed that expression of these miRNAs have the potential to distinguish between psoriasis and controls. At baseline, miRNA-155 expression in PBMCs correlated with Psoriasis Area Severity Index (PASI) [12 (8-14)] (Spearman r: 0.7140, P < 0.05) suggesting a role in psoriasis. After MTX or topical treatment, reduction in PASI was observed [87.5% (75-100)]; miRNA-155 expression in PBMCs decreased; plasma miRNA-21, miRNA-146a and miRNA-223 were down-regulated. ROC analysis showed that miRNA-155 expression in PBMCs from psoriasis patients have the potential to distinguish between patients' samples at baseline and after treatment (AUC: 0.942, sensitivity: 0.91; specificity: 0.91 values; maximum likelihood ratio =10). After treatment, miRNA-146a expression in PBMCs increased; miRNA-155/miRNA-146a ratio decreased, suggestive of a regulatory feedback; let-7i expression decreased; miRNA-21 and miRNA-223 remained elevated.
  
  
  CONCLUSIONS
  In this exploratory study, psoriasis patients presented increased expression of miRNA-155 in PBMCs that correlated with PASI and decreased with disease remission. MiRNA-21, miRNA-146a and miRNA-223 in PBMCs and plasma were increased at baseline and differentially modulated, underscoring different roles of TLR-related miRNAs in psoriasis.
authors.affiliation: Department of Cellular Biology and Immunology, Instituto de Parasitología y Biomedicina López-Neyra, IPBLN-CSIC, Parque Tecnológico de la Salud de Granada (PTS Granada), Av. Conocimiento, 17, Granada-18016, Spain.
authors.forename: S
authors.initials: S
authors.lastname: García-Rodríguez
authors.affiliation: Department of Dermatology, Instituto de Investigación Biosanitaria de Granada, "ibs.GRANADA", Hospitales Universitarios de Granada (H. U. Granada)/Universidad de Granada (UGR), Granada, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Arias-Santiago
authors.affiliation: Department of Dermatology, Instituto de Investigación Biosanitaria de Granada, "ibs.GRANADA", Hospitales Universitarios de Granada (H. U. Granada)/Universidad de Granada (UGR), Granada, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Blasco-Morente
authors.affiliation: Department of Dermatology, Instituto de Investigación Biosanitaria de Granada, "ibs.GRANADA", Hospitales Universitarios de Granada (H. U. Granada)/Universidad de Granada (UGR), Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Orgaz-Molina
authors.affiliation: Department of Cellular Biology and Immunology, Instituto de Parasitología y Biomedicina López-Neyra, IPBLN-CSIC, Parque Tecnológico de la Salud de Granada (PTS Granada), Av. Conocimiento, 17, Granada-18016, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Rosal-Vela
authors.affiliation: Department of Cellular Biology and Immunology, Instituto de Parasitología y Biomedicina López-Neyra, IPBLN-CSIC, Parque Tecnológico de la Salud de Granada (PTS Granada), Av. Conocimiento, 17, Granada-18016, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Navarro
authors.affiliation: Department of Rheumatology, Instituto de Investigación Biosanitaria de Granada, "ibs.GRANADA", Hospitales Universitarios de Granada (H. U. Granada)/Universidad de Granada (UGR), Granada, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Magro-Checa
authors.affiliation: Department of Dermatology, Instituto de Investigación Biosanitaria de Granada, "ibs.GRANADA", Hospitales Universitarios de Granada (H. U. Granada)/Universidad de Granada (UGR), Granada, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Martínez-López
authors.affiliation: Department of Dermatology, Instituto de Investigación Biosanitaria de Granada, "ibs.GRANADA", Hospitales Universitarios de Granada (H. U. Granada)/Universidad de Granada (UGR), Granada, Spain.
authors.forename: J-C
authors.initials: JC
authors.lastname: Ruiz
authors.affiliation: Department of Rheumatology, Instituto de Investigación Biosanitaria de Granada, "ibs.GRANADA", Hospitales Universitarios de Granada (H. U. Granada)/Universidad de Granada (UGR), Granada, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Raya
authors.affiliation: Department of Dermatology, Instituto de Investigación Biosanitaria de Granada, "ibs.GRANADA", Hospitales Universitarios de Granada (H. U. Granada)/Universidad de Granada (UGR), Granada, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Naranjo-Sintes
authors.affiliation: Department of Cellular Biology and Immunology, Instituto de Parasitología y Biomedicina López-Neyra, IPBLN-CSIC, Parque Tecnológico de la Salud de Granada (PTS Granada), Av. Conocimiento, 17, Granada-18016, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Sancho
authors.affiliation: Department of Cellular Biology and Immunology, Instituto de Parasitología y Biomedicina López-Neyra, IPBLN-CSIC, Parque Tecnológico de la Salud de Granada (PTS Granada), Av. Conocimiento, 17, Granada-18016, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Zubiaur
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D009000:Monocytes / Q000378:metabolism*; D011565:Psoriasis / Q000097:blood*
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs