title: Inhibitory Effects of Astragaloside IV on Bleomycin-Induced Pulmonary Fibrosis in Rats Via Attenuation of Oxidative Stress and Inflammation.
abstract: In this study, we investigated the effects of astragaloside IV (As-IV) on pulmonary fibrosis and its mechanisms of action. Sprague-Dawley rats were used in a model of pulmonary fibrosis induced by an intratracheal instillation of bleomycin (BLM). Rats were intraperitoneally injected with As-IV (10, 20, 50 mg/kg) daily for 28 days, while the rats in control and BLM groups were injected with a saline solution. The effects of As-IV treatment on pulmonary injury were evaluated with the lung wet/dry weight ratios, cell counts, and histopathologic. Oxidative stress was evaluated by detecting the levels of malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and reactive oxygen species (ROS) in lung tissue. Inflammation was assessed by measuring the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in bronchoalveolar lavage fluid (BALF). The results indicated that As-IV treatment remarkably ameliorated BLM-induced pulmonary fibrosis and attenuated BLM-induced oxidative stress and inflammation. Our findings indicate that As-IV-mediated suppression of fibroproliferation may contribute to the anti-fibrotic effect against BLM-induced pulmonary fibrosis. Its mechanisms of action are associated with inhibiting oxidative stress and inflammatory response. In summary, our study suggests a therapeutic potential of As-IV in the treatment of pulmonary fibrosis.
authors.affiliation: Department of Respiration, Yantai Hospital of Traditional Chinese Medicine, N0.39 Xingfu Road, Zhifu District, Yantai, 264000, China.
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Yu
authors.affiliation: Department of Respiration, Yantai Hospital of Traditional Chinese Medicine, N0.39 Xingfu Road, Zhifu District, Yantai, 264000, China.
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Sun
authors.affiliation: Department of Respiration, Yantai Hospital of Traditional Chinese Medicine, N0.39 Xingfu Road, Zhifu District, Yantai, 264000, China. yanghua9@hotmail.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D001761:Bleomycin; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000175:diagnosis / Q000188:drug therapy; D018384:Oxidative Stress / Q000187:drug effects; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000175:diagnosis / Q000188:drug therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000008:administration & dosage / Q000494:pharmacology*; D014315:Triterpenes / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D012503:Saponins; D014315:Triterpenes; D001761:Bleomycin; C052064:astragaloside A
keywords: astragaloside IV; inflammation; oxidative stress; pulmonary fibrosis