title: Influences of CCK-8 on expressions of apoptosis-related genes in prefrontal cortex neurons of morphine-relapse rats.
abstract: In order to elucidate the influences of CCK-8 on expressions of apoptosis-related genes, Bax, Bcl-2 and Caspase-3, of prefrontal cortex neurons in morphine-relapse rats, an effective, successful morphine-relapse-rat model using the conditioned place preference (CPP) under CCK-8 (0.01, 0.1 and 1.0μg, i.c.v) intervention was established. The prefrontal cortexes were made into slices with the cellular plasmas immunohistochemically stained. The expressions of Bax, Bcl-2, Caspase-3 of neurons were evaluated through their scores, and each corresponding ratio of Bax and Bcl-2 (Bax/Bcl-2) was also computed. The results showed that the expression of Bcl-2 was very weak and those of Bax and Caspase-3 were hardly seen in group normal saline; the expressions of Bax and Caspase-3 were strong and that of Bcl-2 was weak in group morphine and compared to group normal saline, there were significant differences (P<0.05); the expressions of Bax, Caspase-3 and the ratios of Bax/Bcl-2 have a gradually-decreased trend in the sequence of group 0.01μg, group 0.1μg and group 1.0μg, but the expression of Bcl-2 has an opposite trend in the same sequence, and compared to group morphine, there were significant differences (P<0.05) excluding group 0.01μg. So we draw a conclusion that CCK-8 (0.1 and 1.0μg, i.c.v) could protect neurons of prefrontal cortex through up-regulating the expression of Bcl-2, down-regulating those of Bax and Caspase-3 and reducing Bax/Bcl-2 ratio in the model of morphine-relapse rats.
authors.affiliation: Hebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Hebei Medical University, PR China; Institute of Forensic Sciences, Soochow University, Suzhou, Jiangsu, PR China; Department of Forensic Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, PR China; Institute of Forensic Science, Wenzhou Medical University, Wenzhou, Zhejiang, PR China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Ye
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, Jiangsu, PR China. Electronic address: taoluyang@suda.edu.cn.
authors.forename: Luyang
authors.initials: L
authors.lastname: Tao
authors.affiliation: Hebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Hebei Medical University, PR China. Electronic address: chunlingma@126.com.
authors.forename: Chunling
authors.initials: C
authors.lastname: Ma
authors.affiliation: Hebei Key Laboratory of Forensic Medicine, Department of Forensic Medicine, Hebei Medical University, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Wen
authors.affiliation: Department of Forensic Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, PR China; Institute of Forensic Science, Wenzhou Medical University, Wenzhou, Zhejiang, PR China.
authors.forename: Linsheng
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Forensic Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, PR China; Institute of Forensic Science, Wenzhou Medical University, Wenzhou, Zhejiang, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Forensic Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, PR China; Institute of Forensic Science, Wenzhou Medical University, Wenzhou, Zhejiang, PR China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Hu
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, Jiangsu, PR China.
authors.forename: Xiping
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, Jiangsu, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chu
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, Jiangsu, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, Jiangsu, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Gao
authors.affiliation: Institute of Forensic Sciences, Soochow University, Suzhou, Jiangsu, PR China.
authors.forename: Haochen
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D053148:Caspase 3 / Q000378:metabolism; D003214:Conditioning, Classical / Q000187:drug effects / Q000502:physiology; D008297:Male; D009020:Morphine / Q000008:administration & dosage*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012008:Recurrence; D012844:Sincalide / Q000008:administration & dosage* / Q000502:physiology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D009020:Morphine; C505410:Casp3 protein, rat; D053148:Caspase 3; D012844:Sincalide
keywords: Bax; Bcl-2; Caspase-3; Cholecystokinin-8; Morphine; Morphine relapse