title: Applications of the BLEND Software to Crystallographic Data from Membrane Proteins.
abstract: X-ray diffraction from crystals of membrane proteins very often yields incomplete datasets due to, among other things, severe radiation damage. Multiple crystals are thus required to form complete datasets, provided the crystals themselves are isomorphous. Selection and combination of data from multiple crystals is a difficult and tedious task that can be facilitated by purpose-built software. BLEND, in the CCP4 suite of programs for macromolecular crystallography (MX), has been created exactly for this reason. In this chapter the program is described and its workings illustrated by means of data from two membrane proteins.
authors.affiliation: Diamond Light Source Ltd, Harwell Science and Innovation Campus, OX11 0DE, Didcot, UK.
authors.forename: Pierre
authors.initials: P
authors.lastname: Aller
authors.affiliation: Membrane Protein Laboratory, Diamond Light Source Ltd, Harwell Science and Innovation Campus, OX11 0DE, Didcot, UK.
authors.forename: Tian
authors.initials: T
authors.lastname: Geng
authors.affiliation: Diamond Light Source Ltd, Harwell Science and Innovation Campus, OX11 0DE, Didcot, UK.
authors.forename: Gwyndaf
authors.initials: G
authors.lastname: Evans
authors.affiliation: Diamond Light Source Ltd, Harwell Science and Innovation Campus, OX11 0DE, Didcot, UK. james.foadi@diamond.ac.uk.
authors.forename: James
authors.initials: J
authors.lastname: Foadi
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D003196:Computer Graphics; D018360:Crystallography, X-Ray; D006193:Haemophilus influenzae / Q000737:chemistry; D006801:Humans; D008432:Mathematical Computing; D008565:Membrane Proteins / Q000737:chemistry* / Q000528:radiation effects; D011969:Receptors, Histamine H1 / Q000737:chemistry; D012984:Software
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D011969:Receptors, Histamine H1
keywords: Blend; Cluster analysis; Data merging; Data scaling; Isomorphism; Membrane proteins; Multiple crystals; Radiation damage