title: Surfactant Protein A Recognizes Outer Membrane Protein OprH on Pseudomonas aeruginosa Isolates From Individuals With Chronic Infection.
abstract: Surfactant protein A (SP-A) plays a critical role in the clearance of Pseudomonas aeruginosa from the lung. However, there is limited information about the interaction of this protein with P. aeruginosa isolates from individuals with cystic fibrosis (CF). We characterized the interplay between SP-A and a collection of isogenic sequential isolates from 7 patients with CF. We identified outer membrane protein OprH as a novel ligand for SP-A on P. aeruginosa The last-available (late) isolates from patients with CF bound significantly less SP-A than their respective first-available (early) isolates. This difference could be associated with a reduction in the expression of OprH. Binding of SP-A to OprH promoted phagocytic killing; thus, late CF isolates were at least 2-fold more resistant to SP-A-mediated killing by human macrophages than their respective early isolates. We postulate that the reduction of OprH expression is a previously unrecognized adaptation of P. aeruginosa to the lung of individuals with CF that facilitates the escape of the microorganism from SP-A-mediated phagocytic killing.
authors.affiliation: Instituto Universitario de Investigación en Ciencias de la Salud, Universidad de las Islas Baleares.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Qadi
authors.affiliation: Servicio de Microbiología.|Unidad de Investigación, Hospital Son Espases, Instituto de Investigación Sanitaria de Palma, Palma de Mallorca, Spain.
authors.forename: Carla
authors.initials: C
authors.lastname: Lopez-Causapé
authors.affiliation: Instituto Universitario de Investigación en Ciencias de la Salud, Universidad de las Islas Baleares.
authors.forename: Sofia
authors.initials: S
authors.lastname: Izquierdo-Rabassa
authors.affiliation: Instituto Universitario de Investigación en Ciencias de la Salud, Universidad de las Islas Baleares.
authors.forename: Margalida
authors.initials: M
authors.lastname: Mateu Borrás
authors.affiliation: Department of Pediatrics.|Center for Cystic Fibrosis Research, Emory University School of Medicine, Children's Healthcare of Atlanta, Georgia.
authors.forename: Joanna B
authors.initials: JB
authors.lastname: Goldberg
authors.affiliation: Servicio de Microbiología.|Unidad de Investigación, Hospital Son Espases, Instituto de Investigación Sanitaria de Palma, Palma de Mallorca, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Oliver
authors.affiliation: Instituto Universitario de Investigación en Ciencias de la Salud, Universidad de las Islas Baleares.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Albertí
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000378:metabolism* / Q000382:microbiology*; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000382:microbiology; D011552:Pseudomonas Infections / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D037662:Pulmonary Surfactant-Associated Protein A / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; C402926:OprH protein, Pseudomonas aeruginosa; D037662:Pulmonary Surfactant-Associated Protein A
keywords: Pseudomonas aeruginosa; cystic fibrosis; outer membrane protein OprH; surfactant protein A