title: Crystal Dehydration in Membrane Protein Crystallography.
abstract: Crystal dehydration has been successfully implemented to facilitate the structural solution of a number of soluble and membrane protein structures over the years. This chapter will present the currently available tools to undertake controlled crystal dehydration, focusing on some successful membrane protein cases. Also discussed here will be some practical considerations regarding membrane protein crystals and the relationship between different techniques in order to help researchers to select the most suitable technique for their projects.
authors.affiliation: Diamond Light Source, Harwell Science and innovation Campus, Didcot, OX11 0DE, UK. juan.sanchez-weatherby@diamond.ac.uk.
authors.forename: Juan
authors.initials: J
authors.lastname: Sanchez-Weatherby
authors.affiliation: Membrane Protein Laboratory, Diamond Light Source/Imperial College London, Harwell Campus, Didcot, Oxfordshire, UK.
authors.forename: Isabel
authors.initials: I
authors.lastname: Moraes
mesh_terms: D000388:Air; D000818:Animals; D003460:Crystallization / Q000295:instrumentation / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods*; D003890:Desiccation / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D006813:Humidity; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012996:Solutions; D014867:Water
chemical_list: D008565:Membrane Proteins; D012996:Solutions; D014867:Water
keywords: Crystal dehydration; Free mounting system; HC1; In situ; Membrane proteins; Relative humidity