title: The Genetic Analysis of an Acinetobacter johnsonii Clinical Strain Evidenced the Presence of Horizontal Genetic Transfer.
abstract: Acinetobacter johnsonii rarely causes human infections. While most A. johnsonii isolates are susceptible to virtually all antibiotics, strains harboring a variety of β-lactamases have recently been described. An A. johnsonii Aj2199 clinical strain recovered from a hospital in Buenos Aires produces PER-2 and OXA-58. We decided to delve into its genome by obtaining the whole genome sequence of the Aj2199 strain. Genome comparison studies on Aj2199 revealed 240 unique genes and a close relation to strain WJ10621, isolated from the urine of a patient in China. Genomic analysis showed evidence of horizontal genetic transfer (HGT) events. Forty-five insertion sequences and two intact prophages were found in addition to several resistance determinants such as blaPER-2, blaOXA-58, blaTEM-1, strA, strB, ereA, sul1, aacC2 and a new variant of blaOXA-211, called blaOXA-498. In particular, blaPER-2 and blaTEM-1 are present within the typical contexts previously described in the Enterobacteriaceae family. These results suggest that A. johnsonii actively acquires exogenous DNA from other bacterial species and concomitantly becomes a reservoir of resistance genes.
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPaM, UBA-CONICET), Buenos Aires, Argentina.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Montaña
authors.affiliation: Department of Biological Science, California State University Fullerton, Fullerton, CA, United States of America.
authors.forename: Sareda T J
authors.initials: ST
authors.lastname: Schramm
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPaM, UBA-CONICET), Buenos Aires, Argentina.
authors.forename: German Matías
authors.initials: GM
authors.lastname: Traglia
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPaM, UBA-CONICET), Buenos Aires, Argentina.|Department of Biological Science, California State University Fullerton, Fullerton, CA, United States of America.
authors.forename: Kevin
authors.initials: K
authors.lastname: Chiem
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPaM, UBA-CONICET), Buenos Aires, Argentina.
authors.forename: Gisela
authors.initials: G
authors.lastname: Parmeciano Di Noto
authors.affiliation: Laboratorio de Bacteriología Clínica, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Marisa
authors.initials: M
authors.lastname: Almuzara
authors.affiliation: Laboratorio de Bacteriología Clínica, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Claudia
authors.initials: C
authors.lastname: Barberis
authors.affiliation: Laboratorio de Bacteriología Clínica, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Carlos
authors.initials: C
authors.lastname: Vay
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPaM, UBA-CONICET), Buenos Aires, Argentina.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Quiroga
authors.affiliation: Department of Biological Science, California State University Fullerton, Fullerton, CA, United States of America.
authors.forename: Marcelo E
authors.initials: ME
authors.lastname: Tolmasky
authors.affiliation: Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, UdelaR, Montevideo, Uruguay.
authors.forename: Andrés
authors.initials: A
authors.lastname: Iriarte
authors.affiliation: Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPaM, UBA-CONICET), Buenos Aires, Argentina.|Department of Biological Science, California State University Fullerton, Fullerton, CA, United States of America.
authors.forename: María Soledad
authors.initials: MS
authors.lastname: Ramírez
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology / Q000635:transmission*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001118:Argentina; D001483:Base Sequence; D004197:Disease Reservoirs / Q000382:microbiology*; D015870:Gene Expression; D022761:Gene Transfer, Horizontal; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D006761:Hospitals; D006801:Humans; D008826:Microbial Sensitivity Tests; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D010802:Phylogeny; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D039002:Prophages / Q000235:genetics; D016415:Sequence Alignment; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases