title: MicroRNAs-424 and 206 are potential prognostic markers in spinal onset amyotrophic lateral sclerosis.
abstract: BACKGROUND
  Skeletal muscle microRNAs (miRNAs) are potential candidate biomarkers for amyotrophic lateral sclerosis (ALS) that deserve further investigation.
  
  
  OBJECTIVE
  To identify miRNAs abnormally expressed in the skeletal muscle and plasma of patients with ALS, and to correlate them with parameters of disease progression.
  
  
  METHODS
  Expression profile of miRNAs in muscle was evaluated using an array platform. Subsequently we assessed the plasmatic expression of candidate miRNAs in a set of 39 patients/39 controls. We employed generalized estimating equations to investigate correlations with clinical data.
  
  
  RESULTS
  We identified 11 miRNAs differentially expressed in the muscle of ALS patients; of these, miR424, miR-214 and miR-206 were validated by qPCR in muscle samples. In plasma, we found only miR-424 and miR 206 to be overexpressed. Baseline expression of miR-424 and 206 correlated with clinical deterioration over time.
  
  
  CONCLUSIONS
  MiR-424 and miR-206 are potential prognostic markers for ALS.
authors.affiliation: Department of Neurology, School of Medicine, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: Helen M T
authors.initials: HM
authors.lastname: de Andrade
authors.affiliation: Department of Neurology, School of Medicine, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: Milena
authors.initials: M
authors.lastname: de Albuquerque
authors.affiliation: Department of Medical Genetics, School of Medicine, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: Simoni H
authors.initials: SH
authors.lastname: Avansini
authors.affiliation: Department of Medical Genetics, School of Medicine, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: de S Rocha
authors.affiliation: Department of Medical Genetics, School of Medicine, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: Danyella B
authors.initials: DB
authors.lastname: Dogini
authors.affiliation: Department of Neurology, School of Medicine, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: Anamarli
authors.initials: A
authors.lastname: Nucci
authors.affiliation: Department of Medical Genetics, School of Medicine, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: Benilton
authors.initials: B
authors.lastname: Carvalho
authors.affiliation: Department of Medical Genetics, School of Medicine, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: Iscia
authors.initials: I
authors.lastname: Lopes-Cendes
authors.affiliation: Department of Neurology, School of Medicine, University of Campinas - UNICAMP, Campinas, SP, Brazil. Electronic address: mcfrancajr@uol.com.br.
authors.forename: Marcondes C
authors.initials: MC
authors.lastname: França
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D001774:Blood Chemical Analysis; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012720:Severity of Illness Index; D046888:Tissue Array Analysis
chemical_list: D015415:Biomarkers; C546087:MIRN206 microRNA, human; C554214:MIRN424 microrna, human; D035683:MicroRNAs
keywords: Amyotrophic lateral sclerosis; MicroRNA; Motor neuron; Prognosis; Skeletal muscle