title: Proximal CD13 Versus Distal GATA-3 Expression in Renal Neoplasia According to WHO 2016 Classification.
abstract: Little is known about the aminopeptidase CD13 in renal neoplasia according to the new 2016 World Health Organization renal tumor classification. We selected 175 cases, including 79 clear cell, 31 papillary, 24 chromophobe, 8 clear cell papillary renal cell carcinomas (RCCs), 21 oncoytomas, and 12 microphthalmia transcription factor family translocation RCCs: 4 t(6;11)/transcription factor EB (TFEB), 7 t(Xp11) with 2 cystic variants and 1 t(X;17). GATA binding protein 3 (GATA-3) was inserted as control. Expression of proximal antigen CD13 was observed in 63/79 (80%) clear cell, 25/31 (81%) papillary, 3/8 (37%) clear cell papillary, 1/4 (25%) t(6;11)/TFEB, 2/7 (28%) cystic t(Xp11), and in 1/1 t(X;17) RCCs. All chromophobe RCC (0/24) and all oncocytomas (0/21) resulted negative. CD10 was seen in 76/79 (96%) clear cell, 15/31 (48%) papillary, 10/24 (42%) chromophobe, 1/8 (12%) clear cell papillary RCCs, 4/21 (19%) oncocytomas, 1/4 (25%) t(6;11)/TFEB, 2/7 (29%) cystic t(Xp11), and in 1/1 t(X;17) RCCs. GATA-3 was positive in 3/7 (42%) clear cell papillary RCCs and negative in all remaining RCCs, except a single chromophobe RCC and a single oncocytoma. We concluded that: (1) CD13 and GATA-3 immunostains may serve as a diagnostic aid in differentiating subtypes of RCC; (2) CD13 is always absent in chromophobe RCC and oncocytomas, whereas CD10 can be immunoexpressed in both; (3) CD13 should be included in a panel of antibodies to distinguish "proximal renal tumors" from "distal renal tumors" and between clear cell RCC versus microphthalmia transcription factor family translocations RCCs; and (4) when present, GATA-3 is specific for clear cell papillary RCC.
authors.affiliation: Department of Diagnostics and Public Health, University of Verona, Verona.
authors.forename: Matteo
authors.initials: M
authors.lastname: Brunelli
authors.affiliation: Department of Diagnostics and Public Health, University of Verona, Verona.
authors.forename: Francesco
authors.initials: F
authors.lastname: Erdini
authors.affiliation: Department of Diagnostics and Public Health, University of Verona, Verona.
authors.forename: Luca
authors.initials: L
authors.lastname: Cima
authors.affiliation: Department of Diagnostics and Public Health, University of Verona, Verona.
authors.forename: Albino
authors.initials: A
authors.lastname: Eccher
authors.affiliation: Department of Diagnostics and Public Health, University of Verona, Verona.
authors.forename: Adele
authors.initials: A
authors.lastname: Fioravanzo
authors.affiliation: Department of Diagnostics and Public Health, University of Verona, Verona.
authors.forename: Stefano
authors.initials: S
authors.lastname: Gobbo
authors.affiliation: Department of Diagnostics and Public Health, University of Verona, Verona.
authors.forename: Diego
authors.initials: D
authors.lastname: Segala
authors.affiliation: Department of Diagnostics and Public Health, University of Verona, Verona.
authors.forename: Claudio
authors.initials: C
authors.lastname: Ghimenton
authors.affiliation: Pathology Unit, San Maurizio Hospital, Bolzano.
authors.forename: Guido
authors.initials: G
authors.lastname: Mazzoleni
authors.affiliation: Department of Diagnostics and Public Health, University of Verona, Verona.
authors.forename: Enrico
authors.initials: E
authors.lastname: Munari
authors.affiliation: Pathology Unit, San Maurizio Hospital, Bolzano.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Carella
authors.affiliation: Department of Diagnostics and Public Health, University of Verona, Verona.|Pathology Unit, Pederzoli Hospital, Peschiera Del Garda, Italy.
authors.forename: Guido
authors.initials: G
authors.lastname: Martignoni
mesh_terms: D018249:Adenoma, Oxyphilic / Q000175:diagnosis*; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018826:CD13 Antigens / Q000378:metabolism*; D002291:Carcinoma, Papillary / Q000175:diagnosis*; D002292:Carcinoma, Renal Cell / Q000175:diagnosis*; D003937:Diagnosis, Differential; D050990:GATA3 Transcription Factor / Q000378:metabolism*; D006801:Humans; D007120:Immunochemistry; D007680:Kidney Neoplasms / Q000175:diagnosis*; D008297:Male; D014178:Translocation, Genetic; D014944:World Health Organization
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D014408:Biomarkers, Tumor; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; C064972:TFEB protein, human; D018826:CD13 Antigens