title: Inhibition of PID1/NYGGF4/PCLI1 gene expression highlights its role in the early events of the cell cycle in NIH3T3 fibroblasts.
abstract: The PID1/NYGGF4/PCLI1 gene encodes for a protein with a phosphotyrosine-binding domain, which interacts with the lipoprotein receptor-related protein 1. Previous work by us and others suggested a function of the gene in cell proliferation of NIH3T3 fibroblasts and 3T3-L1 pre-adipocytes. The molecular characterization of PCLI1 protein, ectopically expressed in NIH3T3 fibroblasts, revealed two phosphorylation sites at Ser154 and Ser165. In order to clarify the functions of this gene, we analyzed the effects of its downregulation on cellular proliferation and cell cycle progression in NIH3T3 cell cultures. Downregulation of PID1/NYGGF4/PCLI1 mRNA levels by short hairpin RNAs (shRNAs) elicited decreased proliferation rate in mammalian cell lines; cell cycle analysis of serum-starved, synchronized NIH3T3 fibroblasts showed an increased accumulation of shRNA-interfered cells in the G1 phase. Decreased levels of FOS and MYC mRNAs were accordingly associated with these events. The molecular scenario emerging from our data suggests that PID1/NYGGF4/PCLI1 controls cellular proliferation and cell cycle progression in NIH3T3 cells.
authors.affiliation: a CEINGE Biotecnologie Avanzate , Napoli , Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Monteleone
authors.affiliation: a CEINGE Biotecnologie Avanzate , Napoli , Italy.|b Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II , Napoli , Italy , and.|c Proteomics & Mass Spectrometry Laboratory, ISPAAM - National Research Council , Naples , Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Vitale
authors.affiliation: a CEINGE Biotecnologie Avanzate , Napoli , Italy.|b Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II , Napoli , Italy , and.
authors.forename: Ginevra
authors.initials: G
authors.lastname: Caratù
authors.affiliation: c Proteomics & Mass Spectrometry Laboratory, ISPAAM - National Research Council , Naples , Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: D'Ambrosio
authors.affiliation: b Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II , Napoli , Italy , and.
authors.forename: Stefano
authors.initials: S
authors.lastname: Di Giovanni
authors.affiliation: a CEINGE Biotecnologie Avanzate , Napoli , Italy.
authors.forename: Marisa
authors.initials: M
authors.lastname: Gorrese
authors.affiliation: a CEINGE Biotecnologie Avanzate , Napoli , Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Napolitano
authors.affiliation: b Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II , Napoli , Italy , and.
authors.forename: Maria Fiammetta
authors.initials: MF
authors.lastname: Romano
authors.affiliation: a CEINGE Biotecnologie Avanzate , Napoli , Italy.|b Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II , Napoli , Italy , and.
authors.forename: Luigi
authors.initials: L
authors.lastname: Del Vecchio
authors.affiliation: a CEINGE Biotecnologie Avanzate , Napoli , Italy.|b Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II , Napoli , Italy , and.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Succoio
authors.affiliation: c Proteomics & Mass Spectrometry Laboratory, ISPAAM - National Research Council , Naples , Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Scaloni
authors.affiliation: a CEINGE Biotecnologie Avanzate , Napoli , Italy.|b Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II , Napoli , Italy , and.
authors.forename: Nicola
authors.initials: N
authors.lastname: Zambrano
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D013329:Structure-Activity Relationship
chemical_list: D002352:Carrier Proteins; C556403:NYGGF4 protein, mouse; D034741:RNA, Small Interfering
keywords: Loss-of-function; PTB; shRNA