title: A proteomic analysis of salt stress response in seedlings of two African rice cultivars.
abstract: Salt stress is one of the key abiotic stresses threatening future agricultural production and natural ecosystems. This study investigates the salt stress response of two rice seedlings, which were screened from 28 Kenya rice cultivars. A proteomic analysis was carried out and Mapman bin codes employed in protein function categorization. Proteins in the redox, stress, and signaling categories were identified, and whose expression differed between the salt tolerant and the salt sensitive samples employed in the present study. 104 and 102 root proteins were observed as significantly altered during salt stress in the tolerant and sensitive samples, respectively and 13 proteins were commonly expressed. Among the 13 proteins, ketol-acid reductoisomerase protein was upregulated in both 1 and 3days of salt treatment in the tolerant sample, while it was down-regulated in both 1 and 3days of salt treatment in the sensitive sample. Actin-7, tubulin alpha, V-type proton ATPase, SOD (Cu-Zn), SOD (Mn), and pyruvate decarboxylase were among the observed salt-induced proteins. In general, this study improves our understanding about salt stress response mechanisms in rice.
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan 430074, China; University of Chinese Academy of Sciences, Beijing 100039, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, China.
authors.forename: Rebecca Njeri
authors.initials: RN
authors.lastname: Damaris
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan 430074, China. Electronic address: limit@wbgcas.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan 430074, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Wuhan Institute of Biotechnology, Wuhan 430075, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62,000, 00200 Nairobi, Kenya.
authors.forename: Hunja
authors.initials: H
authors.lastname: Murage
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan 430074, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, China. Electronic address: yangpf@wbgcas.cn.
authors.forename: Pingfang
authors.initials: P
authors.lastname: Yang
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D050540:Ketol-Acid Reductoisomerase / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D011767:Pyruvate Decarboxylase / Q000235:genetics / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1 / Q000235:genetics / Q000378:metabolism; D014404:Tubulin / Q000235:genetics / Q000378:metabolism; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D010940:Plant Proteins; D020543:Proteome; D014404:Tubulin; D012965:Sodium Chloride; D050540:Ketol-Acid Reductoisomerase; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D025262:Vacuolar Proton-Translocating ATPases; D011767:Pyruvate Decarboxylase
keywords: Comparative proteomics; Rice seedlings; Roots; Salt stress