title: Ovary and embryo proteogenomic dataset revealing diversity of vitellogenins in the crustacean Gammarus fossarum.
abstract: Ovaries and embryos from sexually mature Gammarus fossarum were sampled at different stages of the reproductive cycle. The soluble proteome was extracted for five biological replicates and samples were subjected to trypsin digestion. The resulting peptides were analyzed by high resolution tandem mass spectrometry with a LTQ-Orbitrap XL instrument. The MS/MS spectra were assigned with a previously described RNAseq-derived G. fossarum database. The proteins highlighted by proteogenomics were monitored and their abundance kinetics over the different stages revealed a large panel of vitellogenins. Criteria were i) accumulation during oogenesis, ii) decrease during embryogenesis, iii) classified as female-specific, and iv) sequence similarity and phylogenetic analysis. The data accompanying the manuscript describing the database searches and comparative analysis ("High-throughput proteome dynamics for discovery of key proteins in sentinel species: unsuspected vitellogenins diversity in the crustacean Gammarus fossarum" by Trapp et al. [1]) have been deposited to the ProteomeXchange via the PRIDE repository with identifiers PRIDE: PXD001002.
authors.affiliation: Irstea, Unité de Recherche MALY, Laboratoire d'écotoxicologie, CS70077, F-69626 Villeurbanne, France; CEA-Marcoule, DSV/IBITEC-S/SPI/Li2D, Laboratory "Innovative technologies for Detection and Diagnostic", BP 17171, F-30200 Bagnols-sur-Cèze, France.
authors.forename: Judith
authors.initials: J
authors.lastname: Trapp
authors.affiliation: CEA-Marcoule, DSV/IBITEC-S/SPI/Li2D, Laboratory "Innovative technologies for Detection and Diagnostic", BP 17171, F-30200 Bagnols-sur-Cèze, France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Gaillard
authors.affiliation: Irstea, Unité de Recherche MALY, Laboratoire d'écotoxicologie, CS70077, F-69626 Villeurbanne, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Chaumot
authors.affiliation: Irstea, Unité de Recherche MALY, Laboratoire d'écotoxicologie, CS70077, F-69626 Villeurbanne, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Geffard
authors.affiliation: CEA-Marcoule, DSV/IBITEC-S/SPI/Li2D, Laboratory "Innovative technologies for Detection and Diagnostic", BP 17171, F-30200 Bagnols-sur-Cèze, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Pible
authors.affiliation: CEA-Marcoule, DSV/IBITEC-S/SPI/Li2D, Laboratory "Innovative technologies for Detection and Diagnostic", BP 17171, F-30200 Bagnols-sur-Cèze, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Armengaud
keywords: Crustacean; Embryogenesis; Non-model organism; Oogenesis; Proteogenomics Reproduction; Vitellogenins