title: Effects of oxidative stress reaction for the Eisenia fetida with exposure in Cd2.
abstract: Earthworms are widely used in all kinds of pollutants as sensitive bio-indicator organisms because of their immediately oxidative stress response under the stress of heavy metal. However, there are a large number of indexes associated with the oxidative stress response. Finding out the key monitoring indexes in the stress process becomes a practical demand of the pollution monitoring and warning process. We studied two groups, the short-term test and the long-term test. The former one is for 10 days, taking out an earthworm every day. The latter test lasted 30 days, taking out an earthworm every 10 days. The Cd2+concentration was set at 50, 100, 125, 250, and 500 mg kg-1. Post-clitellum segments of earthworms were chosen to determine superoxide enzyme (SOD), peroxidase (POD), glutathione peroxidase (GSH-Px), glutathione-S transferase (GST), catalase (CAT), vitamin E (VE), malondialdehyde (MDA), and acetylcholinesterase (AChE). The results showed that the main bio-indicators associating with oxidative stress reaction in short-term group were CAT, SOD, and POD. MDA could be used as a bio-indicator in the early and mid-term. VE was only the bio-indicator in the mid-term stress. While with the long-term test, the main bio-indicators associated with oxidative stress reaction were GSH-Px and MDA. The AChE activity was only suitable for oxidative stress response caused by heavy metal stress more than 30 days.
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, China. zhboshi@163.com.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Dongxing
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yucui
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Jiabin
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Deng
authors.initials: D
authors.lastname: Jie
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Guohua
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Siqin
authors.initials: S
authors.lastname: Bilige
authors.affiliation: College of Resources and Environmental Science, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yijun
mesh_terms: D000818:Animals; D002104:Cadmium / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D004334:Drug Administration Schedule; D005982:Glutathione Transferase / Q000378:metabolism; D008315:Malondialdehyde; D009835:Oligochaeta / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D012989:Soil Pollutants / Q000737:chemistry / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D012989:Soil Pollutants; D002104:Cadmium; D008315:Malondialdehyde; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
keywords: Cd exposure; Earthworm; Eisenia fetida; Oxidative stress