title: Protective Effect of Dl-3-n-Butylphthalide on Recovery from Cardiac Arrest and Resuscitation in Rats.
abstract: In this study we investigated the effect of Dl-3-n-butylphthalide (NBP), a clinically used drug for stroke patients in China, on the recovery following cardiac arrest and resuscitation in rats. Male Wistar rats (3-month old) underwent cardiac arrest (12 min) and resuscitation. Rats were randomly assigned to the following groups: sham non-arrested group, vehicle group (vehicle-treated, 7 days before cardiac arrest and 4 days post-resuscitation), NBP pre-treated group (NBP-treated, 7 days before cardiac arrest), and NBP post-treated group (NBP-treated, 4 days post-resuscitation). Overall survival rates and hippocampal neuronal counts were determined in each group at 4 days post-resuscitation. Results showed that NBP pre-treated group (80 %) and NBP post-treated group (86 %) had significantly higher survival rates compared to that of the vehicle group (50 %). At 4 days of recovery, only about 20 % of hippocampal neurons were preserved in the vehicle group compared to the sham non-arrested group. The hippocampal CA1 cell counts in the NBP pre-treated group and NBP post-treated group were significantly higher than the counts in the vehicle group, about 50-60 % of the counts of non-arrested rats. The data suggest that NBP has both preventive and therapeutic effect on improving outcome following cardiac arrest and resuscitation, and NBP might be a potential early phase treatment for patients recovered from cardiac arrest and resuscitation.
authors.affiliation: Institute of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Nutrition, Case Western Reserve University, Cleveland, OH, USA.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Puchowicz
authors.affiliation: Department of Physiology and Biophysics, Case Western Reserve University, Robbins Building E732, 10900 Euclid Ave., Cleveland, OH, 44139, USA.
authors.forename: Joseph C
authors.initials: JC
authors.lastname: LaManna
authors.affiliation: Department of Physiology and Biophysics, Case Western Reserve University, Robbins Building E732, 10900 Euclid Ave., Cleveland, OH, 44139, USA. kxx@case.edu.
authors.forename: Kui
authors.initials: K
authors.lastname: Xu
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D006323:Heart Arrest / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D017208:Rats, Wistar; D020127:Recovery of Function; D012151:Resuscitation; D013997:Time Factors
chemical_list: D001572:Benzofurans; D018696:Neuroprotective Agents; C027125:3-n-butylphthalide
keywords: Ischemia/reperfusion injury; Neuroprotection; Post-resuscitation recovery; Selective neuronal death; Transient global ischemia