title: 47kDa isoform of Annexin A7 affecting the apoptosis of mouse hepatocarcinoma cells line.
abstract: Our previous studies have shown that Annexin A7 is associated with the cell proliferation and apoptosis. Both the isoforms 47kDa and 51kDa of Annexin A7 are expressed in liver cancer cells. This study aimed at elucidating which isoform of Annexin A7 affects the cell apoptosis and how to affect in the hepatocarcinoma cell line. The results showed that Annexin A7 co-immunoprecipitated with Bcl2 and co-located in the cytoplasm and the mitochondria. Therein, the regulated 47kDa isoform of Annexin A7 altered the expression of Bcl2 and the mitochondrial membrane potential. These results indicate that 47kDa isoform of Annexin A7 could associated with the apoptosis of Hca-P cells through its interaction with Bcl2.
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Bai
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Huaxin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China. Electronic address: huangyh_1020@163.com.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Pathology, Dalian Medical University, Key Laboratory for Tumor Metastasis of Liaoning Province, Dalian, PR China. Electronic address: jwtang@sina.cn.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Tang
mesh_terms: D000818:Animals; D017310:Annexin A7 / Q000378:metabolism*; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005434:Flow Cytometry; D047468:Immunoprecipitation; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D008970:Molecular Weight; D020033:Protein Isoforms / Q000378:metabolism; D015203:Reproducibility of Results; D015854:Up-Regulation
chemical_list: D017310:Annexin A7; D020033:Protein Isoforms
keywords: 47kDa isoform; Annexin A7; Apoptosis; Hca-P cell