title: Bioinformatic Analysis of Plasma Apolipoproteins A-I and A-II Revealed Unique Features of A-I/A-II HDL Particles in Human Plasma.
abstract: Plasma concentration of apoA-I, apoA-II and apoA-II-unassociated apoA-I was analyzed in 314 Japanese subjects (177 males and 137 females), including one (male) homozygote and 37 (20 males and 17 females) heterozygotes of genetic CETP deficiency. ApoA-I unassociated with apoA-II markedly and linearly increased with HDL-cholesterol, while apoA-II increased only very slightly and the ratio of apoA-II-associated apoA-I to apoA-II stayed constant at 2 in molar ratio throughout the increase of HDL-cholesterol, among the wild type and heterozygous CETP deficiency. Thus, overall HDL concentration almost exclusively depends on HDL with apoA-I without apoA-II (LpAI) while concentration of HDL containing apoA-I and apoA-II (LpAI:AII) is constant having a fixed molar ratio of 2 : 1 regardless of total HDL and apoA-I concentration. Distribution of apoA-I between LpAI and LpAI:AII is consistent with a model of statistical partitioning regardless of sex and CETP genotype. The analysis also indicated that LpA-I accommodates on average 4 apoA-I molecules and has a clearance rate indistinguishable from LpAI:AII. Independent evidence indicated LpAI:A-II has a diameter 20% smaller than LpAI, consistent with a model having two apoA-I and one apoA-II. The functional contribution of these particles is to be investigated.
authors.affiliation: Institute of Environmental Science of Human Life, Ochanomizu University, Bunkyo-ku, Tokyo 112-8610, Japan.
authors.forename: Toshimi
authors.initials: T
authors.lastname: Kido
authors.affiliation: Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, The Jikei University School of Medicine, Nishi-Shimbashi, Minato-ku, Tokyo, 105-8461, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kurata
authors.affiliation: Department of Food and Nutritional Science, Toyo University, Itakura-machi, Ora-gun, Gunma 374-0193, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kondo
authors.affiliation: Shinagawa East One Medical Clinic, Minato-ku, Tokyo 108-0075, Japan.
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Itakura
authors.affiliation: Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8519, Japan.
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Okazaki
authors.affiliation: International Mibyou (Pre Symptomatic Medicine) Medical Center, Sanuki-chou, Ryugasaki, Ibaraki 301-0033, Japan.|Department of Pharmacogenomics, Showa University, Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Takeyoshi
authors.initials: T
authors.lastname: Urata
authors.affiliation: Nutritional Health Science Research Center, Chubu University, Matsumoto-cho, Kasugai 487-8501, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D016632:Apolipoprotein A-I / Q000097:blood*; D016633:Apolipoprotein A-II / Q000097:blood*; D053480:Cholesterol Ester Transfer Proteins / Q000097:blood / Q000172:deficiency / Q000235:genetics; D019295:Computational Biology; D005260:Female; D005838:Genotype; D006801:Humans; D007122:Immunoelectrophoresis; D008052:Lipid Metabolism, Inborn Errors / Q000097:blood / Q000235:genetics; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male
chemical_list: C505693:APOA1 protein, human; C115794:APOA2 protein, human; D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D053480:Cholesterol Ester Transfer Proteins; D008075:Lipoproteins, HDL