title: Assessment of pets (cats and dogs) in homes using electrostatic dust collectors and QPCR: new tools to evaluate exposure and risk of allergies.
abstract: Contradictory results are found in the literature concerning fungi, bacteria, and pet exposure and the risk of developing asthma. All these allergens have been thoroughly studied separately in cohort studies, and a variety of sampling and analytical methods are used. It is already possible to characterize fungi, mites, and bacteria by QPCR. The aim of our study is to evaluate QPCR systems to quantify the presence of cats and dogs in homes. Twenty-four houses were sampled with an Electrostatic Dust Collector which was analyzed by QPCR. Questionnaires on the presence of pets in homes were completed. The results from QPCR were correlated for real presence of cats and dogs, and highlighted indirect exposure. This study provides a useful screening tool that will be used in future large cohort studies, such as the ELFE cohort study.
authors.affiliation: a Chrono-Environnement, UMR/CNRS 6249 , University of Bourgogne-Franche-Comté , Besançon , France.|b Department of Parasitology-Mycology , University Hospital of Besançon , Besançon , France.
authors.forename: Emeline
authors.initials: E
authors.lastname: Scherer
authors.affiliation: a Chrono-Environnement, UMR/CNRS 6249 , University of Bourgogne-Franche-Comté , Besançon , France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Valot
authors.affiliation: a Chrono-Environnement, UMR/CNRS 6249 , University of Bourgogne-Franche-Comté , Besançon , France.
authors.forename: Mallory
authors.initials: M
authors.lastname: Vacheyrou
authors.affiliation: a Chrono-Environnement, UMR/CNRS 6249 , University of Bourgogne-Franche-Comté , Besançon , France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Naegele
authors.affiliation: a Chrono-Environnement, UMR/CNRS 6249 , University of Bourgogne-Franche-Comté , Besançon , France.
authors.forename: Jenny
authors.initials: J
authors.lastname: Knapp
authors.affiliation: a Chrono-Environnement, UMR/CNRS 6249 , University of Bourgogne-Franche-Comté , Besançon , France.|b Department of Parasitology-Mycology , University Hospital of Besançon , Besançon , France.
authors.forename: Steffi
authors.initials: S
authors.lastname: Rocchi
authors.affiliation: a Chrono-Environnement, UMR/CNRS 6249 , University of Bourgogne-Franche-Comté , Besançon , France.|b Department of Parasitology-Mycology , University Hospital of Besançon , Besançon , France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Roussel
authors.affiliation: a Chrono-Environnement, UMR/CNRS 6249 , University of Bourgogne-Franche-Comté , Besançon , France.|b Department of Parasitology-Mycology , University Hospital of Besançon , Besançon , France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Millon
authors.affiliation: a Chrono-Environnement, UMR/CNRS 6249 , University of Bourgogne-Franche-Comté , Besançon , France.|b Department of Parasitology-Mycology , University Hospital of Besançon , Besançon , France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Reboux
mesh_terms: D016902:Air Pollution, Indoor / Q000032:analysis*; D000485:Allergens / Q000032:analysis*; D000818:Animals; D002415:Cats; D004285:Dogs; D004391:Dust / Q000032:analysis*; D004781:Environmental Exposure; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D018570:Risk Assessment
chemical_list: D000485:Allergens; D004391:Dust
keywords: QPCR; antigens; cat; dog; housing