title: Multi-modality Optical Imaging of Rat Kidney Dysfunction: In Vivo Response to Various Ischemia Times.
abstract: We observed in vivo kidney dysfunction with various ischemia times at 30, 75, 90, and 120 min using multi-modality optical imaging: optical coherence tomography (OCT), Doppler OCT (DOCT), and two-photon microscopy (TPM). We imaged the renal tubule lumens and glomerulus at several areas of each kidney before, during, and after ischemia of 5-month-old female Munich-Wistar rats. For animals with 30 and 75 min ischemia times, we observed that all areas were recovered after ischemia, that tubule lumens were re-opened and the blood flow of the glomerulus was re-established. For animals with 90 and 120 min ischemia times, we observed unrecovered areas, and that tubule lumens remained close after ischemia. TPM imaging verified the results of OCT and provided higher resolution images than OCT to visualize renal tubule lumens and glomerulus blood flow at the cellular level.
authors.affiliation: College of Precision Instrument and Opto-Electronics Engineering, Tianjin University, Tianjin, 300072, China.|Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
authors.forename: Zhenyang
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
authors.forename: Lily
authors.initials: L
authors.lastname: Jin
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
authors.forename: Hsing-Wen
authors.initials: HW
authors.lastname: Wang
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
authors.forename: Qinggong
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
authors.forename: Hengchang
authors.initials: H
authors.lastname: Guo
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA. yuchen@umd.edu.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
mesh_terms: D058186:Acute Kidney Injury / Q000000981:diagnostic imaging* / Q000503:physiopathology; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005583:Fourier Analysis; D007678:Kidney Glomerulus / Q000098:blood supply / Q000000981:diagnostic imaging*; D007684:Kidney Tubules / Q000000981:diagnostic imaging* / Q000503:physiopathology; D036641:Microscopy, Fluorescence, Multiphoton; D064847:Multimodal Imaging / Q000379:methods*; D011237:Predictive Value of Tests; D017208:Rats, Wistar; D020127:Recovery of Function; D012079:Renal Circulation; D015427:Reperfusion Injury / Q000000981:diagnostic imaging* / Q000503:physiopathology; D013997:Time Factors; D041623:Tomography, Optical Coherence; D018608:Ultrasonography, Doppler
keywords: Doppler optical coherence tomography; Ischemia; Kidney dysfunction; Optical coherence tomography; Two-photon microscopy