title: In-situ synthesis of AgNPs in the natural/synthetic hybrid nanofibrous scaffolds: Fabrication, characterization and antimicrobial activities.
abstract: Silver nanoparticles embedded within a nanofibrous polymer matrix have significant attention in recent years as an antimicrobial wound dressing materials. Herein, we have fabricated a novel Ag-polyurethane-zein hybrid nanofibrous scaffold for wound dressing applications. AgNPs were synthesized in-situ via reduction of silver nitrate in electrospinning solution. Varying mass composition of the components showed the pronounced effect on the morphology and physicochemical properties of the composite fibers. Field-Emission Scanning Electron Microscopy (FESEM) images revealed that PU and zein with mass ratio 2:1 produced the bead-free continuous and uniformly distributed nanofibers. Fourier-transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and Thermogravimetric Analysis (TGA) confirmed the well interaction between component polymers. Compared to the pristine PU nanofibers, composite fibers showed enhanced tensile strength, young׳s modulus and surface wettability. The antibacterial capacity of the nanofibrous membrane was evaluated against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacterial strains via a zone of inhibition test, and the results showed high antibacterial performance for Ag incorporated composite mat. Experimental results of cell viability assay and microscopic imaging revealed that as-fabricated scaffolds have an excellent ability for fibroblast cell adhesion, proliferation and growth. Overall, as-fabricated antibacterial natural/synthetic composite scaffold can be a promising substrate for repairing skin defects.
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Bikendra
authors.initials: B
authors.lastname: Maharjan
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea; Department of Chemistry, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal.
authors.forename: Mahesh Kumar
authors.initials: MK
authors.lastname: Joshi
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Arjun Prasad
authors.initials: AP
authors.lastname: Tiwari
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea; Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: biochan@jbnu.ac.kr.
authors.forename: Chan Hee
authors.initials: CH
authors.lastname: Park
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea; Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Eco-friendly Machine Parts Design Research Center, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: chskim@jbnu.ac.kr.
authors.forename: Cheol Sang
authors.initials: CS
authors.lastname: Kim
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001458:Bandages; D004926:Escherichia coli / Q000187:drug effects; D053768:Metal Nanoparticles / Q000032:analysis*; D057139:Nanofibers / Q000032:analysis*; D012834:Silver / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D012834:Silver
keywords: Antimicrobial effect; Composite nanofiber scaffolds; Electrospinning; Mechanical property; Solution property; Tissue engineering