title: Measurement of Histone Methylation Dynamics by One-Carbon Metabolic Isotope Labeling and High-energy Collisional Dissociation Methylation Signature Ion Detection.
abstract: Accumulating evidence suggests that cellular metabolites and nutrition levels control epigenetic modifications, including histone methylation. However, it is not currently possible to measure the metabolic control of histone methylation. Here we report a novel detection method to monitor methyl transfer from serine to histones through the one-carbon metabolic pathway, using stable-isotope labeling and detection of lysine methylation signature ions generated in high-energy-dissociation (HCD) tandem mass spectrometry. This method is a long-needed tool to study the metabolic control of histone methylation.
authors.affiliation: Department of Pharmacology &Toxicology, University of Texas Medical Branch, Galveston, Texas, 77555, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
authors.affiliation: Institute for Translational Sciences, UTMB, Galveston, Texas, 77555, USA.|Sealy Center for Molecular Medicine, UTMB, Galveston, Texas, 77555, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Tian
authors.affiliation: Institute for Translational Sciences, UTMB, Galveston, Texas, 77555, USA.|Sealy Center for Molecular Medicine, UTMB, Galveston, Texas, 77555, USA.
authors.forename: Allan R
authors.initials: AR
authors.lastname: Brasier
authors.affiliation: Department of Pharmacology &Toxicology, University of Texas Medical Branch, Galveston, Texas, 77555, USA.
authors.forename: Lawrence C
authors.initials: LC
authors.lastname: Sowers
authors.affiliation: Department of Pharmacology &Toxicology, University of Texas Medical Branch, Galveston, Texas, 77555, USA.|Sealy Center for Molecular Medicine, UTMB, Galveston, Texas, 77555, USA.
authors.forename: Kangling
authors.initials: K
authors.lastname: Zhang
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D006657:Histones / Q000378:metabolism*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D053719:Tandem Mass Spectrometry; D020298:U937 Cells
chemical_list: D006657:Histones; D002244:Carbon; D008239:Lysine