title: Loopβ3αC plays an important role in the structure and function of isocitrate dehydrogenase kinase/phosphatase.
abstract: This work aims to investigate the role of the loopβ3αC amino acids in the structure and function of isocitrate dehydrogenase kinase/phosphatase (AceK). The results demonstrate that the precise configuration of loopβ3αC is very important for AceK structure and function: structural changes alter the affinity of the enzyme for the isocitrate dehydrogenase (ICDH), which modifies enzyme activity. Intriguingly, D340 is significant for the retention of kinase and phosphatase activities, for the conformational stability of AceK and for binding ICDH. The deletion Δ341-345 increases enzyme activity by increasing the maximum velocity and affinity for ICDH. The β3αC loop is thus critical for the structure and function of AceK.
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory, China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory, China.
authors.forename: Shanze
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory, China.
authors.forename: Yadan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tong
authors.affiliation: College of Chemistry, Beijing Normal University, China.
authors.forename: Jimin
authors.initials: J
authors.lastname: Zheng
authors.affiliation: College of Chemistry, Beijing Normal University, China.
authors.forename: Zongchao
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory, China. jgh982@bnu.edu.cn.
authors.forename: Guohua
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory, China. weiq@bnu.edu.cn.
authors.forename: Qun
authors.initials: Q
authors.lastname: Wei
mesh_terms: D020134:Catalytic Domain; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009154:Mutation
chemical_list: D029968:Escherichia coli Proteins; C548023:AceK protein, E coli; D007521:Isocitrate Dehydrogenase
keywords: AceK; deletion mutant; isocitrate dehydrogenase; kinase; loop β3αC; phosphatase