title: Evaluation of Muscle Fiber Types in German Shepherd Dogs of Different Ages.
abstract: The objective of this study was to determine and confirm the percentage of type I and type II muscle fibers that comprise the Gluteus Medius muscle in male and female canines of the German Shepherd breed, with standardized care, in different age groups, using the enzyme histochemical method. Muscle samples were collected from the Gluteus Medius muscles of forty clinically healthy dogs of the German Shepherd breed using the technique of percutaneous needle muscle biopsy. The samples were evaluated using histological and enzyme histochemical methods. The percentages of type I and II fibers and the ratio between the quantity of type I fibers/quantity of type II fibers were evaluated using the parameters of weight, age group, correlation between sex and age group, and between the sexes. It was found that there was no significant difference in relation to the types of fibers for the parameters of weight, age group, and age of the females. The correlation between the ages of the males suggested an increase in the percentage of type I fibers, a decrease in the percentage of type II fibers, or an increase in the ratio during the aging process. It was concluded that there was a decrease in the percentage of type II fibers with advancing age in male dogs, but without significant difference in the percentage of type I and type II fibers in relation to the weight. Anat Rec, 299:1540-1547, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Pathology and Veterinary Clinic, Veterinary School, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Sérgio
authors.initials: S
authors.lastname: de A Braga
authors.affiliation: Department of Pathology and Veterinary Clinic, Veterinary School, Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Felipe
authors.initials: F
authors.lastname: G F Padilha
authors.affiliation: Department of Pathology and Veterinary Clinic, Veterinary School, Universidade Federal Fluminense, Niterói, RJ, Brazil. anatopatovet@vm.uff.br.
authors.forename: Ana
authors.initials: A
authors.lastname: M R Ferreira
mesh_terms: D000367:Age Factors; D000818:Animals; D004285:Dogs; D005260:Female; D006651:Histocytochemistry; D008297:Male; D018656:Muscle Fibers, Fast-Twitch / Q000166:cytology* / Q000378:metabolism; D018657:Muscle Fibers, Slow-Twitch / Q000166:cytology* / Q000378:metabolism; D013848:Thigh
keywords: aging; enzyme histochemical; histology; muscle biopsy