title: Development of Two FhSAP2 Recombinant-Based Assays for Immunodiagnosis of Human Chronic Fascioliasis.
abstract: In the United States, infection with Fasciola hepatica has been identified as an emerging disease, primarily in immigrants, refugees, and travelers. The laboratory test of choice for diagnosis of fascioliasis is detection of disease specific antibodies, most commonly uses excretory-secretory antigens for detection of IgG antibodies. Recently, recombinant proteins such as F. hepatica antigen (FhSAP2) have been used to detect IgG antibodies. The glutathione S-transferase (GST)-FhSAP2 recombinant antigen was used to develop Western blot (WB) and fluorescent bead-based (Luminex) assays to detect F. hepatica total IgG and IgG4antibodies. The sensitivity and specificity of GST-FhSAP2 total IgG and IgG4WB were similar at 94% and 98%, respectively. For the IgG Luminex assay, the sensitivity and specificity were 94% and 97%, and for the IgG4, the values were 100% and 99%, respectively. In conclusion, the GST-FhSAP2 antigen performs well in several assay formats and can be used for clinical diagnosis.
authors.affiliation: Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Shin
authors.affiliation: Emory College, Emory University, Atlanta, Georgia.
authors.forename: Angel
authors.initials: A
authors.lastname: Hsu
authors.affiliation: Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Holly M
authors.initials: HM
authors.lastname: Chastain
authors.affiliation: Laboratory of Immunology and Molecular Parasitology, Department of Microbiology, School of Medicine, University of Puerto Rico, San Juan, Puerto Rico.
authors.forename: Lorna A
authors.initials: LA
authors.lastname: Cruz
authors.affiliation: Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Eric S
authors.initials: ES
authors.lastname: Elder
authors.affiliation: University of Georgia, Athens, Georgia.
authors.forename: Sarah G H
authors.initials: SG
authors.lastname: Sapp
authors.affiliation: Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia.
authors.forename: Isabel
authors.initials: I
authors.lastname: McAuliffe
authors.affiliation: Laboratory of Immunology and Molecular Parasitology, Department of Microbiology, School of Medicine, University of Puerto Rico, San Juan, Puerto Rico.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Espino
authors.affiliation: Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia. ahi0@cdc.gov.
authors.forename: Sukwan
authors.initials: S
authors.lastname: Handali
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D016022:Case-Control Studies; D002908:Chronic Disease; D003429:Cross Reactions / Q000276:immunology; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005982:Glutathione Transferase; D006725:Hookworm Infections / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D014120:Toxocariasis / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D005982:Glutathione Transferase