title: [THE SENSITIVITY AND SPECIFICITY OF THE MOLECULAR GENOTYPE MTBDRPL US ASSAY (HAIN LIFESCIENCE GMBH, GERMANY) FOR RAPID DIAGNOSIS OF DRUG SUSCEPTIBILITY IN MYCOBACTERIUM TUBERCULOSIS ON SPUTUM SPECIMENS].
abstract: The efficiency of the molecular GenoType MTBDRplus (Hain Lifescience GmbH, Germany) assay was evaluated for the rapid diagnosis of rifampicin and isoniazid susceptibility in Mycobacterium tuberculosis (MBT) on 168 sputum specimens from patients with pulmonary tuberculosis in the Samara Region. The interpretable results from the molecular genetic assay were obtained for 91.7% of the specimens with the rates significantly higher in the sputum specimens graded 2+ and 3+ than in those graded 1+. The sensitivity and specificity of the assay for the detection of rifampicin, isoniazid, and multidrug resistance were 96.2%, 97.4%; 97.1% and 90.7%; 83.3%; 88.9%, respectively. Mutations in codon 531 of the rpoB gene and codon 315 of the katG gene were most common in rifampicin- and isoniazid-resistant strains, respectively. The high sensitivity, rapidness-to-use, and the simultaneous treatment of a large number of sputum specimens permit the authors to recommend the GenoType MTBDRplus assay as a major screening tool for the detection of drug resistance in MBT.
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D002374:Catalase / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity; D015203:Reproducibility of Results; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C505805:rpoB protein, Mycobacterium tuberculosis; D002374:Catalase; C480353:katG protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases; D007538:Isoniazid; D012293:Rifampin