title: Large-scale recording of thalamocortical circuits: in vivo electrophysiology with the two-dimensional electronic depth control silicon probe.
abstract: Recording simultaneous activity of a large number of neurons in distributed neuronal networks is crucial to understand higher order brain functions. We demonstrate the in vivo performance of a recently developed electrophysiological recording system comprising a two-dimensional, multi-shank, high-density silicon probe with integrated complementary metal-oxide semiconductor electronics. The system implements the concept of electronic depth control (EDC), which enables the electronic selection of a limited number of recording sites on each of the probe shafts. This innovative feature of the system permits simultaneous recording of local field potentials (LFP) and single- and multiple-unit activity (SUA and MUA, respectively) from multiple brain sites with high quality and without the actual physical movement of the probe. To evaluate the in vivo recording capabilities of the EDC probe, we recorded LFP, MUA, and SUA in acute experiments from cortical and thalamic brain areas of anesthetized rats and mice. The advantages of large-scale recording with the EDC probe are illustrated by investigating the spatiotemporal dynamics of pharmacologically induced thalamocortical slow-wave activity in rats and by the two-dimensional tonotopic mapping of the auditory thalamus. In mice, spatial distribution of thalamic responses to optogenetic stimulation of the neocortex was examined. Utilizing the benefits of the EDC system may result in a higher yield of useful data from a single experiment compared with traditional passive multielectrode arrays, and thus in the reduction of animals needed for a research study.
authors.affiliation: Group of Comparative Psychophysiology, Institute of Cognitive Neuroscience and Psychology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.|Faculty of Information Technology and Bionics, Pázmány Péter, Catholic University, Budapest, Hungary.|School of Ph.D. Studies, Semmelweis University, Budapest, Hungary.
authors.forename: Richárd
authors.initials: R
authors.lastname: Fiáth
authors.affiliation: Faculty of Information Technology and Bionics, Pázmány Péter, Catholic University, Budapest, Hungary.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Beregszászi
authors.affiliation: Group of Comparative Psychophysiology, Institute of Cognitive Neuroscience and Psychology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.|Faculty of Information Technology and Bionics, Pázmány Péter, Catholic University, Budapest, Hungary.|School of Ph.D. Studies, Semmelweis University, Budapest, Hungary.
authors.forename: Domonkos
authors.initials: D
authors.lastname: Horváth
authors.affiliation: Group of Comparative Psychophysiology, Institute of Cognitive Neuroscience and Psychology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Lucia
authors.initials: L
authors.lastname: Wittner
authors.affiliation: ATLAS NeuroEngineering, Leuven, Belgium.
authors.forename: Arno A A
authors.initials: AA
authors.lastname: Aarts
authors.affiliation: Department of Microsystems Engineering (IMTEK), University of Freiburg, Freiburg, Germany.|BrainLinks-BrainTools Cluster of Excellence, University of Freiburg, Freiburg, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Ruther
authors.affiliation: Unitec Semicondutores, Ribeirão das Neves, Brazil.|Solid State Electronics, Department of Engineering Sciences, Uppsala University, Uppsala, Sweden; and.
authors.forename: Hercules P
authors.initials: HP
authors.lastname: Neves
authors.affiliation: Laboratory of Thalamus Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Hajnalka
authors.initials: H
authors.lastname: Bokor
authors.affiliation: Laboratory of Thalamus Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Acsády
authors.affiliation: Group of Comparative Psychophysiology, Institute of Cognitive Neuroscience and Psychology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary; ulbert.istvan@ttk.mta.hu.|Faculty of Information Technology and Bionics, Pázmány Péter, Catholic University, Budapest, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Ulbert
mesh_terms: D000161:Acoustic Stimulation / Q000379:methods; D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D002540:Cerebral Cortex / Q000502:physiology*; D004567:Electrodes, Implanted; D005260:Female; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009415:Nerve Net / Q000502:physiology*; D062308:Optogenetics / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D012825:Silicon; D013788:Thalamus / Q000502:physiology*
chemical_list: D012825:Silicon
keywords: electronic depth control; high-density recording; optogenetics; silicon probe; thalamocortical oscillation