title: Automatic quantitative analysis of experimental primary and secondary retinal neurodegeneration: implications for optic neuropathies.
abstract: Secondary neurodegeneration is thought to play an important role in the pathology of neurodegenerative disease, which potential therapies may target. However, the quantitative assessment of the degree of secondary neurodegeneration is difficult. The present study describes a novel algorithm from which estimates of primary and secondary degeneration are computed using well-established rodent models of partial optic nerve transection (pONT) and ocular hypertension (OHT). Brn3-labelled retinal ganglion cells (RGCs) were identified in whole-retinal mounts from which RGC density, nearest neighbour distances and regularity indices were determined. The spatial distribution and rate of RGC loss were assessed and the percentage of primary and secondary degeneration in each non-overlapping segment was calculated. Mean RGC number (82 592±681) and RGC density (1695±23.3 RGC/mm(2)) in naïve eyes were comparable with previous studies, with an average decline in RGC density of 71±17 and 23±5% over the time course of pONT and OHT models, respectively. Spatial analysis revealed greatest RGC loss in the superior and central retina in pONT, but significant RGC loss in the inferior retina from 3 days post model induction. In comparison, there was no significant difference between superior and inferior retina after OHT induction, and RGC loss occurred mainly along the superior/inferior axis (~30%) versus the nasal-temporal axis (~15%). Intriguingly, a significant loss of RGCs was also observed in contralateral eyes in experimental OHT. In conclusion, a novel algorithm to automatically segment Brn3a-labelled retinal whole-mounts into non-overlapping segments is described, which enables automated spatial and temporal segmentation of RGCs, revealing heterogeneity in the spatial distribution of primary and secondary degenerative processes. This method provides an attractive means to rapidly determine the efficacy of neuroprotective therapies with implications for any neurodegenerative disorder affecting the retina.
authors.affiliation: Glaucoma and Retinal Neurodegeneration Research, Visual Neuroscience, UCL Institute of Ophthalmology , Bath Street, London EC1V 9EL, UK.
authors.forename: B M
authors.initials: BM
authors.lastname: Davis
authors.affiliation: Glaucoma and Retinal Neurodegeneration Research, Visual Neuroscience, UCL Institute of Ophthalmology , Bath Street, London EC1V 9EL, UK.
authors.forename: L
authors.initials: L
authors.lastname: Guo
authors.affiliation: Glaucoma and Retinal Neurodegeneration Research, Visual Neuroscience, UCL Institute of Ophthalmology , Bath Street, London EC1V 9EL, UK.
authors.forename: J
authors.initials: J
authors.lastname: Brenton
authors.affiliation: Glaucoma and Retinal Neurodegeneration Research, Visual Neuroscience, UCL Institute of Ophthalmology , Bath Street, London EC1V 9EL, UK.
authors.forename: L
authors.initials: L
authors.lastname: Langley
authors.affiliation: Western Eye Hospital, Imperial College Healthcare Trust , London, UK.
authors.forename: E M
authors.initials: EM
authors.lastname: Normando
authors.affiliation: Glaucoma and Retinal Neurodegeneration Research, Visual Neuroscience, UCL Institute of Ophthalmology, Bath Street, London EC1V 9EL, UK; Western Eye Hospital, Imperial College Healthcare Trust, London, UK.
authors.forename: M F
authors.initials: MF
authors.lastname: Cordeiro