title: Expression of tissue factor and forkhead box transcription factor O-1 in a rat model for chronic thromboembolic pulmonary hypertension.
abstract: Few reports have examined tissue factor (TF) and forkhead box transcription factor O-1 (FoxO1) expression in chronic thromboembolic pulmonary hypertension (CTEPH) animal models. To investigate the role of TF and FoxO1 and their interactions during CTEPH pathogenesis in a rat model. Autologous blood clots were repeatedly injected into the pulmonary arteries through right jugular vein to induce a rat model of CTEPH. Hemodynamic parameters, histopathology, and TF and FoxO1expression levels were detected. The mean pulmonary arterial pressure (mPAP), pulmonary vascular resistance and vessel wall area/total area (WA/TA) ratio in the experiment group increased significantly than sham group (P < 0.05). The cardiac output in the 1-, 2-, and 4-week groups decreased significantly (P < 0.05) when compared to sham group. TF mRNA expression levels in the experiment group increased significantly than sham group (P < 0.05). FoxO1 mRNA and protein expression levels were lower in the experiment group than sham group (P < 0.05). The mPAP had a positive correlation with WA/TA ratio (r = 0.45, P = 0.01). TF mRNA expression had a positive correlation with WA/TA ratio (r = 0.374, P = 0.035) and a positive correlation with mPAP (r = 0.48, P= 0.005). FoxO1 mRNA expression had a negative correlation trend with the WA/TA ratio (r = -0.297, P = 0.099) and a negative correlation trend with mPAP (r = -0.34, P = 0.057). TF mRNA expression had a negative correlation with FoxO1 mRNA expression (r = -0.62, P < 0.001). A rat model of CTEPH can be successfully established by the injection of autologous blood clots into the pulmonary artery. TF and FoxO1 may play a key role in vascular remodeling during CTEPH pathogenesis.
authors.affiliation: Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, Fujian, China. jasonsci7333@163.com.
authors.forename: Chaosheng
authors.initials: C
authors.lastname: Deng
authors.affiliation: Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, Fujian, China.
authors.forename: Dawen
authors.initials: D
authors.lastname: Wu
authors.affiliation: Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, Fujian, China.
authors.forename: Minxia
authors.initials: M
authors.lastname: Yang
authors.affiliation: Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, Fujian, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, Fujian, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, Fujian, China.
authors.forename: Zhanghua
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, Fujian, China.
authors.forename: Ningfang
authors.initials: N
authors.lastname: Lian
authors.affiliation: Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, Fujian, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.affiliation: Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, Fujian, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006976:Hypertension, Pulmonary / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D011655:Pulmonary Embolism / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013925:Thromboplastin / Q000096:biosynthesis*; D014655:Vascular Resistance; D000071161:Forkhead Box Protein O1
chemical_list: D009419:Nerve Tissue Proteins; D013925:Thromboplastin; C074639:Foxo1 protein, rat; D000071161:Forkhead Box Protein O1
keywords: Animal model; FoxO1; Pulmonary hypertension; Pulmonary thromboembolism; Tissue factor