title: GLP-1 nanomedicine alleviates gut inflammation.
abstract: The gut hormone, glucagon like peptide-1 (GLP-1) exerts anti-inflammatory effects. However, its clinical use is limited by its short half-life. Previously, we have shown that GLP-1 as a nanomedicine (GLP-1 in sterically stabilized phospholipid micelles, GLP-1-SSM) has increased in vivo stability. The current study was aimed at testing the efficacy of this GLP-1 nanomedicine in alleviating colonic inflammation and associated diarrhea in dextran sodium sulfate (DSS) induced mouse colitis model. Our results show that GLP-1-SSM treatment markedly alleviated the colitis phenotype by reducing the expression of pro-inflammatory cytokine IL-1β, increasing goblet cells and preserving intestinal epithelial architecture in colitis model. Further, GLP-1-SSM alleviated diarrhea (as assessed by luminal fluid) by increasing protein expression of intestinal chloride transporter DRA (down regulated in adenoma). Our results indicate that GLP-1 nanomedicine may act as a novel therapeutic tool in alleviating gut inflammation and associated diarrhea in inflammatory bowel disease (IBD).
authors.affiliation: Department of Medicine, Division of Gastroenterology and Hepatology, College of Medicine, University of Illinois, Chicago, IL, USA.
authors.forename: Arivarasu N
authors.initials: AN
authors.lastname: Anbazhagan
authors.affiliation: Department of Biopharmaceutical Sciences, College of Pharmacy, University of Illinois, Chicago, IL, USA.
authors.forename: Mentor
authors.initials: M
authors.lastname: Thaqi
authors.affiliation: Department of Medicine, Division of Gastroenterology and Hepatology, College of Medicine, University of Illinois, Chicago, IL, USA.
authors.forename: Shubha
authors.initials: S
authors.lastname: Priyamvada
authors.affiliation: Department of Biopharmaceutical Sciences, College of Pharmacy, University of Illinois, Chicago, IL, USA.
authors.forename: Dulari
authors.initials: D
authors.lastname: Jayawardena
authors.affiliation: Department of Medicine, Division of Gastroenterology and Hepatology, College of Medicine, University of Illinois, Chicago, IL, USA.
authors.forename: Anoop
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Medicine, Division of Gastroenterology and Hepatology, College of Medicine, University of Illinois, Chicago, IL, USA.
authors.forename: Tarunmeet
authors.initials: T
authors.lastname: Gujral
authors.affiliation: Department of Medicine, Division of Gastroenterology and Hepatology, College of Medicine, University of Illinois, Chicago, IL, USA.
authors.forename: Ishita
authors.initials: I
authors.lastname: Chatterjee
authors.affiliation: Department of Medicine, Division of Gastroenterology and Hepatology, College of Medicine, University of Illinois, Chicago, IL, USA.
authors.forename: Edurne
authors.initials: E
authors.lastname: Mugarza
authors.affiliation: Department of Medicine, Division of Gastroenterology and Hepatology, College of Medicine, University of Illinois, Chicago, IL, USA.
authors.forename: Seema
authors.initials: S
authors.lastname: Saksena
authors.affiliation: Department of Biopharmaceutical Sciences, College of Pharmacy, University of Illinois, Chicago, IL, USA.
authors.forename: Hayat
authors.initials: H
authors.lastname: Onyuksel
authors.affiliation: Department of Medicine, Division of Gastroenterology and Hepatology, College of Medicine, University of Illinois, Chicago, IL, USA; Jesse Brown VA Medical Center, Chicago, IL, USA. Electronic address: pkdudeja@uic.edu.
authors.forename: Pradeep K
authors.initials: PK
authors.lastname: Dudeja
mesh_terms: D000818:Animals; D003092:Colitis / Q000188:drug therapy*; D016264:Dextran Sulfate / Q000627:therapeutic use; D003967:Diarrhea / Q000188:drug therapy / Q000209:etiology; D004195:Disease Models, Animal; D052216:Glucagon-Like Peptide 1 / Q000008:administration & dosage* / Q000627:therapeutic use; D007249:Inflammation; D051379:Mice; D050997:Nanomedicine
chemical_list: D052216:Glucagon-Like Peptide 1; D016264:Dextran Sulfate
keywords: Colitis; Diarrhea; GLP-1 SSM; IBD