title: The water soluble peripherally tetra-substituted zinc(ii), manganese(iii) and copper(ii) phthalocyanines as new potential anticancer agents.
abstract: In this study, [2-(2-morpholin-4-ylethoxy)ethoxy] group substituted zinc(ii), manganese(iii) and copper(ii) phthalocyanines 2-4 and their water soluble derivatives 2a, 3a and 4a were synthesized and the interactions of compounds 2a, 3a and 4a with CT-DNA and supercoiled pBR322 plasmid DNA were investigated. The results of binding experiments showed that these compounds were able to interact with CT-DNA via intercalative mode with a strong binding affinity in the order 3a > 2a > 4a. DNA-photocleavage activities of compounds 2a, 3a and 4a were determined. These compounds cleaved supercoiled pBR322 plasmid DNA efficiently under irradiation at 650 nm for 2a and 4a, and at 750 nm for 3a. These compounds displayed remarkable inhibitory activities against topoisomerase I enzyme in a dose-dependent manner. All of these results suggest that these phthalocyanines might be suitable anticancer agents due to their strong binding affinities, significant cleavage activities and effective topoisomerase I inhibition.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080 Trabzon, Turkey.
authors.forename: Burak
authors.initials: B
authors.lastname: Barut
authors.forename: Ayşenur
authors.initials: A
authors.lastname: Sofuoğlu
authors.forename: Zekeriya
authors.initials: Z
authors.lastname: Biyiklioglu
authors.forename: Arzu
authors.initials: A
authors.lastname: Özel
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000528:radiation effects; D003300:Copper / Q000737:chemistry* / Q000528:radiation effects; D004247:DNA / Q000737:chemistry; D053837:DNA Cleavage / Q000187:drug effects / Q000528:radiation effects; D004278:DNA, Superhelical / Q000737:chemistry / Q000528:radiation effects; D007211:Indoles / Q000737:chemistry* / Q000528:radiation effects; D054833:Isoindoles; D008027:Light; D008345:Manganese / Q000737:chemistry* / Q000528:radiation effects; D010778:Photochemotherapy; D012995:Solubility; D014867:Water / Q000737:chemistry; D015032:Zinc / Q000737:chemistry* / Q000528:radiation effects
chemical_list: D000970:Antineoplastic Agents; D004278:DNA, Superhelical; D007211:Indoles; D054833:Isoindoles; D014867:Water; D008345:Manganese; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D015032:Zinc; C013647:phthalocyanine