title: Interleukin-27 is elevated in sepsis-induced myocardial dysfunction and mediates inflammation.
abstract: Interleukin (IL)-27 is an important cytokine involved in many human inflammatory diseases. In this study, we investigated its role in the pathogenesis of sepsis-induced myocardial dysfunction (SIMD).
  
  
  
  Twenty patients with SIMD and 24healthy donors were prospectively enrolled. Expression of IL-27 was detected in serum from SIMD patients by ELISA. Cardiac dysfunction was induced by administration of Escherichia coli lipopolysaccharide (LPS) to C57BL/6 (wild type) or IL-27R-/-mice. IL-27 mRNA in the myocardium was measured by RT-PCR. Cytokine levels in serum were determined by ELISA.
  
  
  
  Expression of IL-27 in the serum was markedly increased in patients with SIMD compared with that in controls. Serum IL-27 levels and cardiac IL-27 mRNA expression were significantly increased after LPS injection compared with control specimens. Compared with wild-type mice, IL-27R-/-mice had higher expression of brain natriuretic peptide, cardiac troponin I, IL-6, IL-12, tumor necrosis factor-α and transforming growth factor-β.
  
  
  
  IL-27 is an important protective mediator of SIMD.
authors.affiliation: Department of Anesthesia, Stomatology Hospital of Chongqing Medical University, No. 426 Songshibei Road, Yubei District, Chongqing 401146, China. Electronic address: gaofeng61647093@126.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China; Department of Critical Care Medicine, The Affiliated Hospital of Hainan Medical College, No. 31 Hualong Road, Xinhua District, Hainan 571101, China. Electronic address: 362383118@qq.com.
authors.forename: Yuan-Zheng
authors.initials: YZ
authors.lastname: Yang
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China. Electronic address: 625764852@qq.com.
authors.forename: Xuan-Yun
authors.initials: XY
authors.lastname: Feng
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China. Electronic address: 358097112@qq.com.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Fan
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China. Electronic address: 276173466@qq.com.
authors.forename: Long
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China. Electronic address: guo-rui5@163.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China. Electronic address: liuqiong0219@163.com.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D009202:Cardiomyopathies / Q000097:blood* / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D007378:Interleukins / Q000097:blood* / Q000235:genetics; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018805:Sepsis / Q000097:blood* / Q000139:chemically induced / Q000235:genetics / Q000473:pathology
chemical_list: C486767:Il27 protein, mouse; D007378:Interleukins; D008070:Lipopolysaccharides; C464565:MYDGF protein, human; D012333:RNA, Messenger
keywords: Inflammation; Interleukin-27; Sepsis; Sepsis-induced myocardial dysfunction