title: Quick method for separating target compounds from the bark of Maqian (Zanthoxylum myriacanthum var. pubescens) by high-performance countercurrent chromatography.
abstract: Choosing a suitable solvent system for a countercurrent chromatography separation presents a challenge for many researchers. In this study, we introduce a quick method of separating a target compound from the bark of Zanthoxylum myriacanthum var. pubescens by countercurrent chromatography. This method relies on the thin-layer chromatography based generally useful estimation of solvent systems. This paper will present how to quickly choose a suitable solvent system with a thin-layer chromatography based generally useful estimation of solvent systems working chart. O-Methyltembamide (1) was enriched by countercurrent chromatography using n-hexane/ethyl acetate/methanol/water (6:4:6:4) as the solvent system. Further purification was achieved by high-performance liquid chromatography with purities of 98.2% from Z. myriacanthum var. pubescens bark.
authors.affiliation: Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, P. R. China.
authors.forename: Qingfei
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, P. R. China.|Department of Biology University of Chinese Academy of Sciences, Beijing, P. R. China.
authors.forename: Huanli
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, P. R. China.
authors.forename: Huabin
authors.initials: H
authors.lastname: Hu
authors.affiliation: Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, P. R. China.
authors.forename: Youkai
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, P. R. China. songqs@xtbg.ac.cn.
authors.forename: Qishi
authors.initials: Q
authors.lastname: Song
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003377:Countercurrent Distribution; D006586:Hexanes; D064209:Phytochemicals / Q000302:isolation & purification*; D024301:Plant Bark / Q000737:chemistry*; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: D006586:Hexanes; D064209:Phytochemicals; C026385:n-hexane
keywords: High-performance countercurrent chromatography; O-Methyltembamide; Zanthoxylum myriacanthum var. pubescens