title: Mesenchymal stem cells growth and proliferation enhancement using PLA vs PCL based nanofibrous scaffolds.
abstract: Electrospinning of polymers is the most commonly used technique for nanofiber fabrication. polylactic acid (PLA) and polycaprolactone (PCL) have been shown to be ideal for nanofiber preparation in various biomedical applications, due to characteristics such as biodegradablity and their ability to promote the cell growth, similar to native tissues. The aim of this study was to develop biocompatible and biodegradable PLA and PCL-based nanofibrous scaffolds for enhancing stem cell growth and proliferation. The scaffolds were prepared by electrospinning, and their physicochemical properties were studied using Fourier Transform Infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and X-ray diffraction (XRD). The surface morphology of the developed scaffolds was determined using scanning electron microscopy (SEM). Mesenchymal stem cells (MSCs), derived from both adipose tissue and bone marrow, were seeded onto the prepared nanofibrous scaffolds. The effect of scaffold type, and structural characteristics on survival and proliferation of MSCs were evaluated. Our results show that after full physicochemical characterization of PCL and PLA nanofibrous scaffolds both were safe and non-toxic to the evaluated cells and both scaffolds supported cell attachment and proliferation of bone marrow and adipose tissue-derived MSCs.
authors.affiliation: Centre of Excellence of Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Egypt.
authors.forename: Narguess H
authors.initials: NH
authors.lastname: Marei
authors.affiliation: Centre for Materials Science, Zewail City of Science and Technology, Egypt.
authors.forename: Ibrahim M
authors.initials: IM
authors.lastname: El-Sherbiny
authors.affiliation: Centre of Excellence of Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Lotfy
authors.affiliation: Centre of Excellence of Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: El-Badawy
authors.affiliation: Centre of Excellence of Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Egypt. Electronic address: nelbadri@zewailcity.edu.eg.
authors.forename: Nagwa
authors.initials: N
authors.lastname: El-Badri
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media; D011091:Polyesters; C016240:polycaprolactone; C033616:poly(lactide)
keywords: Biocompatible; Electrospinning; Polymers