title: Remnant lipoprotein size distribution profiling via dynamic light scattering analysis.
abstract: BACKGROUND
  Remnant lipoproteins (RLP) are a metabolically derived subpopulation of triglyceride-rich lipoproteins (TRL) in human blood that are involved in the metabolism of dietary fats or triglycerides. RLP, the smaller and denser variants of TRL particles, are strongly correlated with cardiovascular disease (CVD) and were listed as an emerging atherogenic risk factor by the AHA in 2001.
  
  
  METHODS
  Varying analytical techniques used in clinical studies in the size determination of RLP contribute to conflicting hypotheses in regard to whether larger or smaller RLP particles contribute to CVD progression, though multiple pathways may exist.
  
  
  RESULTS
  We demonstrated a unique combinatorial bioanalytical approach involving the preparative immunoseparation of RLP, and dynamic light scattering for size distribution analysis.
  
  
  CONCLUSIONS
  This is a new facile and robust methodology for the size distribution analysis of RLP that in conjunction with clinical studies may reveal the mechanisms by which RLP cause CVD progression.
authors.affiliation: University of St. Thomas, Department of Chemistry and Physics, 3800 Montrose Blvd., Houston, TX 77006, United States. Electronic address: chandrr@stthom.edu.
authors.forename: Richa
authors.initials: R
authors.lastname: Chandra
authors.affiliation: University of St. Thomas, Department of Chemistry and Physics, 3800 Montrose Blvd., Houston, TX 77006, United States.
authors.forename: Birgit
authors.initials: B
authors.lastname: Mellis
authors.affiliation: University of St. Thomas, Department of Chemistry and Physics, 3800 Montrose Blvd., Houston, TX 77006, United States.
authors.forename: Kyana
authors.initials: K
authors.lastname: Garza
authors.affiliation: University of St. Thomas, Department of Chemistry and Physics, 3800 Montrose Blvd., Houston, TX 77006, United States.
authors.forename: Samee A
authors.initials: SA
authors.lastname: Hameed
authors.affiliation: University of St. Thomas, Department of Chemistry and Physics, 3800 Montrose Blvd., Houston, TX 77006, United States.
authors.forename: James M
authors.initials: JM
authors.lastname: Jurica
authors.affiliation: University of St. Thomas, Department of Chemistry and Physics, 3800 Montrose Blvd., Houston, TX 77006, United States.
authors.forename: Ana V
authors.initials: AV
authors.lastname: Hernandez
authors.affiliation: University of St. Thomas, Department of Chemistry and Physics, 3800 Montrose Blvd., Houston, TX 77006, United States.
authors.forename: Mia N
authors.initials: MN
authors.lastname: Nguyen
authors.affiliation: Houston Community College, 555 Community College Drive, Houston, TX 77013, United States.
authors.forename: Chandra K
authors.initials: CK
authors.lastname: Mittal
mesh_terms: D000067493:Dynamic Light Scattering; D006801:Humans; D008074:Lipoproteins / Q000032:analysis*; D015394:Molecular Structure; D010316:Particle Size
chemical_list: D008074:Lipoproteins
keywords: DLS; Dynamic light scattering; Lipoprotein; Lipoprotein size; RLP; Remnant lipoprotein; Remnant-like lipoproteins; Size distribution; Size distribution profile