title: Trans-cinnamaldehyde protected PC12 cells against oxygen and glucose deprivation/reperfusion (OGD/R)-induced injury via anti-apoptosis and anti-oxidative stress.
abstract: Ischemia stroke is the major cause of mortality and permanent neurological disability with little definitive therapeutic options. This cerebral ischemic injury leads to the oxidative stress and eventually cell death. We hypothesized that treatment of this condition with the trans-cinnamaldehyde(TC) could protect cells from ischemic and reperfusion injury. Oxygen and glucose deprivation/reperfusion (OGD/R) was used as an in vitro model of hypoxic ischemic injury in present study. MTT was used to evaluate the protective effects of TC. Next, we tested whether TC reduced the production of reactive oxygen species (ROS). Besides, experiments were performed to determine whether or not the mitochondrial membrane potential was affected. Furthermore, the inhibiters of NO and PI3 K were used to determine the initial mechanisms. TC treatment improved cell viability, reduced intracellular ROS, and increased MMP. Further, the inhibition of NO or PI3 K significantly reduced TC's protective effects. These findings suggest that TC might be a promising agent for ischemic stroke.
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Ningxia Hui Autonomous Region, 1160 Shengli Street, Yinchuan, 750004, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Qi
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Ningxia Hui Autonomous Region, 1160 Shengli Street, Yinchuan, 750004, People's Republic of China.
authors.forename: Ru
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Ningxia Hui Autonomous Region, 1160 Shengli Street, Yinchuan, 750004, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Ningxia Hui Autonomous Region, 1160 Shengli Street, Yinchuan, 750004, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Ningxia Hui Autonomous Region, 1160 Shengli Street, Yinchuan, 750004, People's Republic of China.
authors.forename: Wan-Nian
authors.initials: WN
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, Peking University, Health Science Center, Beijing, 100191, People's Republic of China.
authors.forename: Huan-Ran
authors.initials: HR
authors.lastname: Tan
authors.affiliation: Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Ningxia Hui Autonomous Region, Yinchuan, 750004, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Ningxia Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Ningxia Hui Autonomous Region, 750004, Yinchuan, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Ningxia Hui Autonomous Region, 1160 Shengli Street, Yinchuan, 750004, People's Republic of China. li_yuxiang@163.com.|College of Nursing, Ningxia Medical University, Ningxia Hui Autonomous Region, 750004, Yinchuan, People's Republic of China. li_yuxiang@163.com.
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Ningxia Hui Autonomous Region, 1160 Shengli Street, Yinchuan, 750004, People's Republic of China. yujq910315@163.com.|Ningxia Hui Medicine Modern Engineering Research Center and Collaborative Innovation Center, Ningxia Medical University, Ningxia Hui Autonomous Region, Yinchuan, 750004, People's Republic of China. yujq910315@163.com.
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Yu
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010100:Oxygen / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D000171:Acrolein; D005947:Glucose; D010100:Oxygen; C012843:cinnamaldehyde
keywords: Apoptosis; Ischemic injury; Oxidative stress; Oxygen and glucose deprivation; PC12 cells; Trans-cinnamaldehyde