title: Increased mannoprotein content in wines produced by Saccharomyces kudriavzevii×Saccharomyces cerevisiae hybrids.
abstract: Several wine quality aspects are influenced by yeast mannoproteins on account of aroma compounds retention, lactic-acid bacterial growth stimulation, protection against protein haze and astringency reduction. Thus selecting a yeast strain that produces high levels of mannoproteins is important for the winemaking industry. In this work, we observed increased levels of mannoproteins in S. cerevisiae×S. kudriavzevii hybrids, compared to the S. cerevisiae strain, in wine fermentations. Furthermore, the expression of a key gene related to mannoproteins biosynthesis, PMT1, increased in the S. cerevisiae×S. kudriavzevii hybrid. We showed that artificially constructed S. cerevisiae×S. kudriavzevii hybrids also increased the levels of mannoproteins. This work demonstrates that either natural or artificial S. cerevisiae×S. kudriavzevii hybrids present mannoprotein overproducing capacity under winemaking conditions, a desirable physiological feature for this industry. These results suggest that genome interaction in hybrids generates a physiological environment that enhances the release of mannoproteins.
authors.affiliation: Instituto de Agroquímica y Tecnología de los Alimentos, IATA-CSIC, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Pérez-Través
authors.affiliation: Instituto de Agroquímica y Tecnología de los Alimentos, IATA-CSIC, Spain.
authors.forename: Amparo
authors.initials: A
authors.lastname: Querol
authors.affiliation: Instituto de Agroquímica y Tecnología de los Alimentos, IATA-CSIC, Spain. Electronic address: rober@iata.csic.es.
authors.forename: Roberto
authors.initials: R
authors.lastname: Pérez-Torrado
mesh_terms: D005285:Fermentation; D005818:Genetic Engineering; D016681:Genome, Fungal; D006824:Hybridization, Genetic; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000378:metabolism; D012440:Saccharomyces / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014920:Wine / Q000032:analysis* / Q000382:microbiology
chemical_list: D008562:Membrane Glycoproteins; C025363:mannoproteins
keywords: Hybrids; PMT1; Polysaccharides; Saccharomyces; Winemaking