title: Protease regulation and capacity during Caulobacter growth.
abstract: Cell growth requires the removal of proteins that are unwanted or toxic. In bacteria, AAA+ proteases like the Clp family and Lon selectively destroy proteins defined by intrinsic specificity or adaptors. Caulobacter crescentus is a gram-negative bacterium that undergoes an obligate developmental transition every cell division cycle. Here we highlight recent work that reveals how a hierarchy of adaptors targets the degradation of key proteins at specific times during this cell cycle, integrating protein destruction with other cues. We describe recent insight into how Caulobacter manages DNA replication and repair through Lon and Clp proteases. Because proteases must manage a broad substrate repertoire there must be methods to compensate for protease saturation and we discuss these scenarios.
authors.affiliation: Department of Biochemistry and Molecular Biology, Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst, USA.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Vass
authors.affiliation: Department of Biochemistry and Molecular Biology, Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst, USA.
authors.forename: Rilee D
authors.initials: RD
authors.lastname: Zeinert
authors.affiliation: Department of Biochemistry and Molecular Biology, Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst, USA. Electronic address: pchien@biochem.umass.edu.
authors.forename: Peter
authors.initials: P
authors.lastname: Chien
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016935:Caulobacter crescentus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D002453:Cell Cycle / Q000235:genetics; D002455:Cell Division; D004261:DNA Replication / Q000235:genetics; D004269:DNA, Bacterial; D049071:Endopeptidase Clp / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D010447:Peptide Hydrolases / Q000235:genetics* / Q000378:metabolism; D049070:Protease La / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010447:Peptide Hydrolases; D049070:Protease La; D049071:Endopeptidase Clp