title: Protective effects of a blueberry extract in acute inflammation and collagen-induced arthritis in the rat.
abstract: Here we investigated the anti-inflammatory effect of a blueberry extract in the carrageenan-induced paw edema model and collagen-induced arthritis model, both in rats. Along with the chemical characterization of the phenolic content of the fruits and extract, the antioxidant potential of the extract, the cellular antioxidant activity and the effects over neutrophils' oxidative burst, were studied in order to provide a mechanistic insight for the anti-inflammatory effects observed. The extract significantly inhibited paw edema formation in an acute model the rat. Our results also demonstrate that the standardized extract had pharmacological activity when administered orally in the collagen-induced arthritis model in the rat and was able to significantly reduce the development of clinical signs of arthritis and the degree of bone resorption, soft tissue swelling and osteophyte formation, consequently improving articular function in treated animals.
authors.affiliation: iMED.ULisboa and Faculdade de Farmácia - Universidade de Lisboa, Avenida Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
authors.forename: Maria-Eduardo
authors.initials: ME
authors.lastname: Figueira
authors.affiliation: iMED.ULisboa and Faculdade de Farmácia - Universidade de Lisboa, Avenida Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
authors.forename: Mónica
authors.initials: M
authors.lastname: Oliveira
authors.affiliation: iMED.ULisboa and Faculdade de Farmácia - Universidade de Lisboa, Avenida Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
authors.forename: Rosa
authors.initials: R
authors.lastname: Direito
authors.affiliation: iMED.ULisboa and Faculdade de Farmácia - Universidade de Lisboa, Avenida Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
authors.forename: João
authors.initials: J
authors.lastname: Rocha
authors.affiliation: Faculdade de Medicina, Universidade de Coimbra, 3004-504 Coimbra, Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Alves
authors.affiliation: ITQB/IBET, Avenida da República, Quinta do Marquês, Estação Agronómica Nacional, 2781-901 Oeiras, Portugal.
authors.forename: Ana-Teresa
authors.initials: AT
authors.lastname: Serra
authors.affiliation: ITQB/IBET, Avenida da República, Quinta do Marquês, Estação Agronómica Nacional, 2781-901 Oeiras, Portugal.
authors.forename: Catarina
authors.initials: C
authors.lastname: Duarte
authors.affiliation: ITQB/IBET, Avenida da República, Quinta do Marquês, Estação Agronómica Nacional, 2781-901 Oeiras, Portugal.
authors.forename: Rosário
authors.initials: R
authors.lastname: Bronze
authors.affiliation: iMED.ULisboa and Faculdade de Farmácia - Universidade de Lisboa, Avenida Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
authors.forename: Adelaide
authors.initials: A
authors.lastname: Fernandes
authors.affiliation: iMED.ULisboa and Faculdade de Farmácia - Universidade de Lisboa, Avenida Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
authors.forename: Dora
authors.initials: D
authors.lastname: Brites
authors.affiliation: REQUIMTE, Laboratório de Química Aplicada, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, 4050-313 Porto, Portugal.
authors.forename: Marisa
authors.initials: M
authors.lastname: Freitas
authors.affiliation: REQUIMTE, Laboratório de Química Aplicada, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, 4050-313 Porto, Portugal.
authors.forename: Eduarda
authors.initials: E
authors.lastname: Fernandes
authors.affiliation: iMED.ULisboa and Faculdade de Farmácia - Universidade de Lisboa, Avenida Prof. Gama Pinto, 1649-003 Lisboa, Portugal. Electronic address: bsepodes@ff.ulisboa.pt.
authors.forename: Bruno
authors.initials: B
authors.lastname: Sepodes
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000473:pathology / Q000517:prevention & control*; D029798:Blueberry Plants; D018938:Caco-2 Cells; D002351:Carrageenan / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D005638:Fruit; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D020011:Protective Agents / Q000302:isolation & purification / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D020011:Protective Agents; D002351:Carrageenan
keywords: Anti-inflammatory; Blueberry fruit; Collagen induced arthritis; Oxidative burst; Vaccinium corymbosum L.