title: Proteins differentially expressed during limonene biotransformation by Penicillium digitatum DSM 62840 were examined using iTRAQ labeling coupled with 2D-LC-MS/MS.
abstract: This study focused on the differences in protein expression at various periods during limonene biotransformation by Penicillium digitatum DSM 62840. A total of 3644 protein-species were quantified by iTRAQ during limonene biotransformation (0 and 12 h). A total of 643 proteins were differentially expressed, 316 proteins were significantly up-regulated and 327 proteins were markedly down-regulated. GO, COG, and pathway enrichment analysis showed that the differentially expressed proteins possessed catalytic and binding functions and were involved in a variety of cellular and metabolic process. Furthermore, the enzymes involved in limonene transformation might be related to cytochrome P-450. This study provided a powerful platform for further exploration of biotransformation, and the identified proteins provided insight into the mechanism of limonene transformation.
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jing-Nan
authors.initials: JN
authors.lastname: Ren
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Yan-Long
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Li
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Tai
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Yang
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Si-Yi
authors.initials: SY
authors.lastname: Pan
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. fangang@mail.hzau.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Fan
mesh_terms: D001711:Biotransformation; D002853:Chromatography, Liquid; D053138:Cyclohexenes / Q000737:chemistry / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D000077222:Limonene; D010407:Penicillium / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D053138:Cyclohexenes; D005656:Fungal Proteins; D013729:Terpenes; D000077222:Limonene
keywords: Biotransformation; Penicillium digitatum DSM 62840; Proteomics; Terpene; iTRAQ