title: Biological Activities of the Essential Oil from Erigeron floribundus.
abstract: Erigeron floribundus (Asteraceae) is an herbaceous plant widely used in Cameroonian traditional medicine to treat various diseases of microbial and non-microbial origin. In the present study, we evaluated the in vitro biological activities displayed by the essential oil obtained from the aerial parts of E. floribundus, namely the antioxidant, antimicrobial and antiproliferative activities. Moreover, we investigated the inhibitory effects of E. floribundus essential oil on nicotinate mononucleotide adenylyltransferase (NadD), a promising new target for developing novel antibiotics, and Trypanosoma brucei, the protozoan parasite responsible for Human African trypanosomiasis. The essential oil composition was dominated by spathulenol (12.2%), caryophyllene oxide (12.4%) and limonene (8.8%). The E. floribundus oil showed a good activity against Staphylococcus aureus (inhibition zone diameter, IZD of 14 mm, minimum inhibitory concentration, MIC of 512 µg/mL). Interestingly, it inhibited the NadD enzyme from S. aureus (IC50 of 98 µg/mL), with no effects on mammalian orthologue enzymes. In addition, T. brucei proliferation was inhibited with IC50 values of 33.5 µg/mL with the essential oil and 5.6 µg/mL with the active component limonene. The essential oil exhibited strong cytotoxicity on HCT 116 colon carcinoma cells with an IC50 value of 14.89 µg/mL, and remarkable ferric reducing antioxidant power (tocopherol-equivalent antioxidant capacity, TEAC = 411.9 μmol·TE/g).
authors.affiliation: School of Pharmacy, University of Camerino, Camerino 62032, Italy. riccardo.petrelli@unicam.it.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Petrelli
authors.affiliation: Department of Clinical Sciences, Section of Biochemistry, Polytechnic University of Marche, Ancona 60131, Italy. orsomando@univpm.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Orsomando
authors.affiliation: Department of Clinical Sciences, Section of Biochemistry, Polytechnic University of Marche, Ancona 60131, Italy. l.sorci@univpm.it.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Sorci
authors.affiliation: School of Pharmacy, University of Camerino, Camerino 62032, Italy. filippo.maggi@unicam.it.
authors.forename: Filippo
authors.initials: F
authors.lastname: Maggi
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University, Umeå 90187, Sweden. farahnaz.ranjbarian@umu.se.
authors.forename: Farahnaz
authors.initials: F
authors.lastname: Ranjbarian
authors.affiliation: Laboratory of Medicinal Plant Biochemistry, Food Science and Nutrition, Department of Biochemistry, Faculty of Sciences, University of Dschang, PO Box 67, Dschang, Cameroon. prbiapa@yahoo.fr.
authors.forename: Prosper C
authors.initials: PC
authors.lastname: Biapa Nya
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino 62032, Italy. dezemona.petrelli@unicam.it.
authors.forename: Dezemona
authors.initials: D
authors.lastname: Petrelli
authors.affiliation: School of Pharmacy, University of Camerino, Camerino 62032, Italy. luca.vitali@unicam.it.
authors.forename: Luca A
authors.initials: LA
authors.lastname: Vitali
authors.affiliation: School of Pharmacy, University of Camerino, Camerino 62032, Italy. giulio.lupidi@unicam.it.
authors.forename: Giulio
authors.initials: G
authors.lastname: Lupidi
authors.affiliation: School of Pharmacy, University of Camerino, Camerino 62032, Italy. luana.quassinti@unicam.it.
authors.forename: Luana
authors.initials: L
authors.lastname: Quassinti
authors.affiliation: School of Pharmacy, University of Camerino, Camerino 62032, Italy. massimo.bramucci@unicam.it.
authors.forename: Massimo
authors.initials: M
authors.lastname: Bramucci
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University, Umeå 90187, Sweden. anders.hofer@umu.se.
authors.forename: Anders
authors.initials: A
authors.lastname: Hofer
authors.affiliation: School of Pharmacy, University of Camerino, Camerino 62032, Italy. loredana.cappellacci@unicam.it.
authors.forename: Loredana
authors.initials: L
authors.lastname: Cappellacci
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D049919:Erigeron / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D051379:Mice; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D009822:Oils, Volatile; D010936:Plant Extracts
keywords: Erigeron floribundus; NadD; Trypanosoma brucei; antimicrobial; antioxidant; caryophyllene oxide; cytotoxicity; essential oil; limonene