title: 4α-Methylated steroids with cytotoxic activity from the soft coral Litophyton mollis.
abstract: Seven new (1-3, 5 and 8-10) and three previously reported (4, 6 and 7) 4α-methylated steroids were isolated from the organic extract of the gorgonian Litophyton mollis. The structures and the relative configurations of the isolated natural products were determined on the basis of extensive analyses of their NMR and MS data. Metabolites 1 and 5-8 exhibited cytotoxic activity against K562 human chronic myelogenous leukemia cells with IC50values below 10μM, while at the same time displaying low toxicity against healthy PBMCs.
authors.affiliation: Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb 10000, Croatia.
authors.forename: Marijana
authors.initials: M
authors.lastname: Zovko Končić
authors.affiliation: Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece.
authors.forename: Efstathia
authors.initials: E
authors.lastname: Ioannou
authors.affiliation: Laboratoire de Biologie Moléculaire et Cellulaire du Cancer (LBMCC), Luxembourg.
authors.forename: Wamtinga Richard
authors.initials: WR
authors.lastname: Sawadogo
authors.affiliation: Natural Products Chemistry Department, National Research Center, Dokki, Cairo 12622, Egypt.
authors.forename: Ayman F
authors.initials: AF
authors.lastname: Abdel-Razik
authors.affiliation: Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece.
authors.forename: Constantinos
authors.initials: C
authors.lastname: Vagias
authors.affiliation: Laboratoire de Biologie Moléculaire et Cellulaire du Cancer (LBMCC), Luxembourg; Department of Pharmacy, College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Marc
authors.initials: M
authors.lastname: Diederich
authors.affiliation: Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece. Electronic address: roussis@pharm.uoa.gr.
authors.forename: Vassilios
authors.initials: V
authors.lastname: Roussis
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000166:cytology* / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013256:Steroids / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D013256:Steroids
keywords: 4α-Methylated steroids; Cytotoxic activity; Litophyton mollis; Structure elucidation