title: DNA Microarray Highlights Nrf2-Mediated Neuron Protection Targeted by Wasabi-Derived Isothiocyanates in IMR-32 Cells.
abstract: 6-(Methylsulfinyl)hexyl isothiocyanate (6-MSITC), 6-(methylthio)hexyl isothiocyanate (6-MTITC), and 4-(methylsulfinyl)butyl isothiocyanate (4-MSITC) are isothiocyanate (ITC) bioactive compounds from Japanese Wasabi. Previous in vivo studies highlighted the neuroprotective potential of ITCs since ITCs enhance the production of antioxidant-related enzymes. Thus, in this present study, a genome-wide DNA microarray analysis was designed to profile gene expression changes in a neuron cell line, IMR-32, stimulated by these ITCs. Among these ITCs, 6-MSITC caused the expression changes of most genes (263), of which 100 genes were upregulated and 163 genes were downregulated. Gene categorization showed that most of the differentially expressed genes are involved in oxidative stress response, and pathway analysis further revealed that Nrf2-mediated oxidative stress pathway is the top of the ITC-modulated signaling pathway. Finally, real-time polymerase chain reaction (PCR) and Western blotting confirmed the gene expression and protein products of the major targets by ITCs. Taken together, Wasabi-derived ITCs might target the Nrf2-mediated oxidative stress pathway to exert neuroprotective effects.
authors.affiliation: The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.
authors.forename: Phoebe Zapanta
authors.initials: PZ
authors.lastname: Trio
authors.affiliation: Department of Food Sciences and Biotechnology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Fujisaki
authors.affiliation: The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.; Present address: Program for Leading Graduate Schools, Health Life Science, Kumamoto University, Kumamoto, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Tanigawa
authors.affiliation: The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.
authors.forename: Ayami
authors.initials: A
authors.lastname: Hisanaga
authors.affiliation: The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.; Department of Food Sciences and Biotechnology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan.
authors.forename: Kozue
authors.initials: K
authors.lastname: Sakao
authors.affiliation: The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.; Department of Food Sciences and Biotechnology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan.
authors.forename: De-Xing
authors.initials: DX
authors.lastname: Hou
keywords: Nrf2 pathway; Wasabi; gene profile analysis; isothiocyanates; sulforaphane