title: DNA Origami Rotaxanes: Tailored Synthesis and Controlled Structure Switching.
abstract: Mechanically interlocked supramolecular assemblies are appealing building blocks for creating functional nanodevices. Herein, we describe the multistep assembly of large DNA origami rotaxanes that are capable of programmable structural switching. We validated the topology and structural integrity of these rotaxanes by analyzing the intermediate and final products of various assembly routes by electrophoresis and electron microscopy. We further analyzed two structure-switching behaviors of our rotaxanes, which are both mediated by DNA hybridization. In the first mechanism, the translational motion of the macrocycle can be triggered or halted at either terminus. In the second mechanism, the macrocycle can be elongated after completion of the rotaxane assembly, giving rise to a unique structure that is otherwise difficult to access.
authors.affiliation: Department of Cell Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516, USA.
authors.forename: John T
authors.initials: JT
authors.lastname: Powell
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516, USA.
authors.forename: Benjamin O
authors.initials: BO
authors.lastname: Akhuetie-Oni
authors.affiliation: Department of Cell Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516, USA.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology & Nanobiology Institute, Yale University, 850 West Campus Drive, West Haven, CT, 06516, USA. chenxiang.lin@yale.edu.
authors.forename: Chenxiang
authors.initials: C
authors.lastname: Lin
mesh_terms: D004247:DNA / Q000737:chemistry*; D047028:Macrocyclic Compounds / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D043862:Rotaxanes / Q000737:chemistry*
chemical_list: D047028:Macrocyclic Compounds; D043862:Rotaxanes; D004247:DNA
keywords: DNA nanotechnology; DNA origami; macrocycles; molecular devices; rotaxanes