title: KDM6B histone demethylase is an epigenetic regulator of estrogen receptor β expression in human pleural mesothelioma.
abstract: To assess the correlation between KDM6B and estrogen receptor β (ERβ) expression in malignant pleural mesothelioma (MPM).
  
  
  
  We evaluated gene expression by in silico analysis of microarray data, real-time PCR and western blot in MPM tumors and cell lines.
  
  
  
  We report a strong positive correlation between the expression of KDM6B and ERβ in MPM tumors and cell lines. We describe that, in hypoxia, the HIF2α-KDM6B axis induces an epithelioid morphology and ERβ expression in biphasic MPM cells with estrogen receptor-negative phenotype. Reduced histone H3K27 tri-methylation confirms KDM6B activity under hypoxic conditions. Importantly, cells treated during reoxygenation with the selective ERβ agonist, KB9520, maintain ERβ expression and the less aggressive phenotype acquired in hypoxia.
authors.affiliation: Department of Pharmaceutical Sciences, University of Piemonte Orientale, Lgo Donegani 2, 28100 Novara, Italy.
authors.forename: Arcangela G
authors.initials: AG
authors.lastname: Manente
authors.forename: Giulia
authors.initials: G
authors.lastname: Pinton
authors.affiliation: Department of Pharmaceutical Sciences, University of Piemonte Orientale, Lgo Donegani 2, 28100 Novara, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Zonca
authors.affiliation: Laboratory of Cellular Biochemistry & Molecular Biology, CRIBENS, Catholic University of the Sacred Heart, 20145 Milan, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Tavian
authors.affiliation: Sidra Medical & Research Center, P.O. Box 26999 Doha, Qatar.
authors.forename: Tanwir
authors.initials: T
authors.lastname: Habib
authors.affiliation: Sidra Medical & Research Center, P.O. Box 26999 Doha, Qatar.
authors.forename: Puthen V
authors.initials: PV
authors.lastname: Jithesh
authors.affiliation: University of Leicester & Leicester University Hospitals, LE1 9HN, Leicester, UK.
authors.forename: Dean
authors.initials: D
authors.lastname: Fennell
authors.affiliation: Department of Biosciences & Nutrition, Karolinska Institutet, S-141 57 Huddinge, Sweden.
authors.forename: Stefan
authors.initials: S
authors.lastname: Nilsson
authors.affiliation: Department of Pharmaceutical Sciences, University of Piemonte Orientale, Lgo Donegani 2, 28100 Novara, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Moro
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D044127:Epigenesis, Genetic; D047629:Estrogen Receptor beta / Q000235:genetics* / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics* / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D008654:Mesothelioma / Q000235:genetics* / Q000378:metabolism; D000086002:Mesothelioma, Malignant; D008745:Methylation; D011499:Protein Processing, Post-Translational; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D047629:Estrogen Receptor beta; D006657:Histones; D056484:Jumonji Domain-Containing Histone Demethylases; D000099162:Endothelial PAS Domain-Containing Protein 1; C524220:KDM6B protein, human
keywords: ERβ; H3K27me3; KDM6B; epigenetic; human malignant pleural mesothelioma