title: Increased long noncoding RNA SNHG20 predicts poor prognosis in colorectal cancer.
abstract: Long noncoding RNAs (lncRNAs) have been suggested to be involved in the development and progression of malignancies. However, the investigation of small nucleolar RNA host gene 20 (SNHG20) on cancer progression remains unknown. The present study aims to explore the clinical significance of SNHG20 and its potential molecular mechanism in colorectal cancer (CRC).
  
  
  
  Quantitative real-time PCR (qRT-PCR) was used to measure the SNHG20 expression in a total of 107 CRC tissues and CRC cell lines. Loss of function approach was employed to explore the biological roles of SNHG20 in vitro. Its potential molecular mechanism was further verified by western blotting and qRT-PCR.
  
  
  
  The results suggested that SNHG20 expression was significantly upregulated in CRC tissues compared to corresponding normal tissues from 107 CRC patients. High expression of SNHG20 was remarkably associated with advanced TNM stage in patients with CRC. Multivariate analyses unraveled that SNHG20 expression was an independent prognostic factor for overall survival in CRC patients. Further functional assays revealed that knockdown of SNHG20 suppressed cell proliferation, invasion and migration, and cell cycle progression in CRC cells. Moreover, SNHG20 regulated cell growth through modulation of a series of cell cycle-associated genes.
  
  
  
  Our findings suggest that dysregulation of SNHG20 participates in CRC progression and may serve as a potential therapeutic target in CRC patients.
authors.affiliation: Department of Minimally Invasive Surgery, The People's Hospital of Chizhou, Chizhou, 247000, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Minimally Invasive Surgery, The People's Hospital of Chizhou, Chizhou, 247000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Minimally Invasive Surgery, The People's Hospital of Chizhou, Chizhou, 247000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Minimally Invasive Surgery, The People's Hospital of Chizhou, Chizhou, 247000, China.
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Fang
authors.affiliation: Department of Minimally Invasive Surgery, The People's Hospital of Chizhou, Chizhou, 247000, China.
authors.forename: Shao-Wen
authors.initials: SW
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, First Hospital Affiliated to Anhui Medical University, Hefei, 230022, China. xiongmmahmu@126.com.
authors.forename: Mao-Ming
authors.initials: MM
authors.lastname: Xiong
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D008297:Male; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D016019:Survival Analysis; D015854:Up-Regulation
chemical_list: D062085:RNA, Long Noncoding; C000623141:long non-coding RNA SNHG20, human
keywords: Cell cycle; Colorectal cancer; Long noncoding RNA; SNHG20