title: Oral Administration of Fermented Soymilk Products Protects the Skin of Hairless Mice against Ultraviolet Damage.
abstract: The protective effect of isoflavones on skin damage from ultraviolet (UV) radiation and their bioavailability were investigated in ovariectomized hairless mice fed diets composed of fermented soymilk containing aglycone forms of isoflavones or control soymilk containing glucose-conjugated forms of isoflavones. The erythema intensity of dorsal skin was significantly higher in ovariectomized mice than in sham-operated mice (p < 0.05). The erythema intensity and epidermal thickness of dorsal skin were significantly lower in the fermented soymilk diet group than in the control diet group (each p < 0.05). Levels of cyclobutane pyrimidine dimers in dorsal skin were significantly lower in the fermented soymilk diet group than in the control group (p < 0.05). Serum and dorsal skin isoflavone concentrations were significantly higher in the fermented soymilk diet group than in the soymilk diet group (p < 0.05). These results indicate that oral administration of a fermented soymilk diet increases isoflavone concentrations in the blood and skin, effectively scavenging the reactive oxygen species generated by UV irradiation and exerting an estrogen-like activity, with a consequent protective effect on skin photodamage in hairless mice.
authors.affiliation: Yakult Central Institute, Tokyo 186-8650, Japan. mitsuyoshi-kano@yakult.co.jp.
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Kano
authors.affiliation: Yakult Central Institute, Tokyo 186-8650, Japan. norihiro-kubotal@yakult.com.jp.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Kubota
authors.affiliation: Yakult Central Institute, Tokyo 186-8650, Japan. norie-masuoka@yakult.co.jp.
authors.forename: Norie
authors.initials: N
authors.lastname: Masuoka
authors.affiliation: Yakult Central Institute, Tokyo 186-8650, Japan. tetsuji-hori@yakult.co.jp.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Hori
authors.affiliation: Yakult Central Institute, Tokyo 186-8650, Japan. koji-miyazaki@yakult.co.jp.
authors.forename: Kouji
authors.initials: K
authors.lastname: Miyazaki
authors.affiliation: Yakult Central Institute, Tokyo 186-8650, Japan. fumiyasu-ishikawa@yakult.co.jp.
authors.forename: Fumiyasu
authors.initials: F
authors.lastname: Ishikawa
mesh_terms: D000284:Administration, Oral; D000818:Animals; D017484:Dermatitis, Phototoxic / Q000517:prevention & control*; D005260:Female; D005285:Fermentation; D051379:Mice; D008812:Mice, Hairless; D010052:Ovariectomy; D011897:Random Allocation; D041942:Soy Milk / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D014466:Ultraviolet Rays / Q000009:adverse effects*
keywords: fermented soymilk; isoflavone; oxidative DNA damage; skin thickness; soymilk; ultraviolet radiation