title: Surface Colonization and Activity of the 2,6-Dichlorobenzamide (BAM) Degrading Aminobacter sp. Strain MSH1 at Macro- and Micropollutant BAM Concentrations.
abstract: Aminobacter sp. MSH1 uses the groundwater micropollutant 2,6-dichlorobenzamide (BAM) as a C and N source and is a potential catalyst for biotreatment of BAM-contaminated groundwater in filtration units of drinking water treatment plants (DWTPs). The oligotrophic environment of DWTPs including trace pollutant concentrations, and the high flow rates impose challenges for micropollutant biodegradation in DWTPs. To understand how trace BAM concentrations affect MSH1 surface colonization and BAM degrading activity, MSH1 was cultivated in flow channels fed continuously with BAM macro- and microconcentrations in a N- and C-limiting medium. At all BAM concentrations, MSH1 colonized the flow channel. BAM degradation efficiencies were concentration-dependent, ranging between 70 and 95%. Similarly, BAM concentration affected surface colonization, but at 100 μg/L BAM and lower, colonization was similar to that in systems without BAM, suggesting that assimilable organic carbon and nitrogen other than those supplied by BAM sustained colonization at BAM microconcentrations. Comparison of specific BAM degradation rates in flow channels and in cultures of suspended freshly grown cells indicated that starvation conditions in flow channels receiving BAM microconcentrations resulted into MSH1 biomasses with 10-100-times reduced BAM degrading activity and provided a kinetic model for predicting BAM degradation under continuous C and N starvation.
authors.affiliation: Division of Soil and Water Management, KU Leuven , Kasteelpark Arenberg 20, BE-3001 Leuven, Belgium.
authors.forename: Aswini
authors.initials: A
authors.lastname: Sekhar
authors.affiliation: Division of Soil and Water Management, KU Leuven , Kasteelpark Arenberg 20, BE-3001 Leuven, Belgium.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Horemans
authors.affiliation: Department of Geochemistry, Geological Survey of Greenland and Denmark (GEUS) , DK-1350 Copenhagen, Denmark.
authors.forename: Jens
authors.initials: J
authors.lastname: Aamand
authors.affiliation: Department of Geochemistry, Geological Survey of Greenland and Denmark (GEUS) , DK-1350 Copenhagen, Denmark.
authors.forename: Sebastian R
authors.initials: SR
authors.lastname: Sørensen
authors.affiliation: Department of Veterinary Public Health and Food Safety, Laboratory of Chemical Analysis, UGent , BE-9000 Ghent, Belgium.
authors.forename: Lynn
authors.initials: L
authors.lastname: Vanhaecke
authors.affiliation: Department of Veterinary Public Health and Food Safety, Laboratory of Chemical Analysis, UGent , BE-9000 Ghent, Belgium.
authors.forename: Julie Vanden
authors.initials: JV
authors.lastname: Bussche
authors.affiliation: Molecular Imaging and Photonics, KU Leuven , Celestijnenlaan 200 F, BE-3001 Leuven, Belgium.
authors.forename: Johan
authors.initials: J
authors.lastname: Hofkens
authors.affiliation: Division of Soil and Water Management, KU Leuven , Kasteelpark Arenberg 20, BE-3001 Leuven, Belgium.
authors.forename: Dirk
authors.initials: D
authors.lastname: Springael
mesh_terms: D001673:Biodegradation, Environmental; D005374:Filtration; D060587:Groundwater; D059127:Phyllobacteriaceae / Q000378:metabolism*; D018508:Water Purification