title: Transcriptional Regulation of Aerobic Metabolism in Pichia pastoris Fermentation.
abstract: In this study, we investigated the classical fermentation process in Pichia pastoris based on transcriptomics. We utilized methanol in pichia yeast cell as the focus of our study, based on two key steps: limiting carbon source replacement (from glycerol to methonal) and fermentative production of exogenous proteins. In the former, the core differential genes in co-expression net point to initiation of aerobic metabolism and generation of peroxisome. The transmission electron microscope (TEM) results showed that yeast gradually adapted methanol induction to increased cell volume, and decreased density, via large number of peroxisomes. In the fermentative production of exogenous proteins, the Gene Ontology (GO) mapping results show that PAS_chr2-1_0582 played a vital role in regulating aerobic metabolic drift. In order to confirm the above results, we disrupted PAS_chr2-1_0582 by homologous recombination. Alcohol consumption was equivalent to one fifth of the normal control, and fewer peroxisomes were observed in Δ0582 strain following methanol induction. In this study we determined the important core genes and GO terms regulating aerobic metabolic drift in Pichia, as well as developing new perspectives for the continued development within this field.
authors.affiliation: Institute of Frontier Medical Science of Jilin University, Changchun 130021, P.R. China.
authors.forename: Biao
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Frontier Medical Science of Jilin University, Changchun 130021, P.R. China.
authors.forename: Baizhi
authors.initials: B
authors.lastname: Li
authors.affiliation: NovelBio Bio-Pharm Technology Co., Ltd, Shanghai 200000, P.R. China.
authors.forename: Dai
authors.initials: D
authors.lastname: Chen
authors.affiliation: NovelBio Bio-Pharm Technology Co., Ltd, Shanghai 200000, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zong
authors.affiliation: Institute of Frontier Medical Science of Jilin University, Changchun 130021, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
authors.affiliation: Institute of Frontier Medical Science of Jilin University, Changchun 130021, P.R. China.
authors.forename: Huixin
authors.initials: H
authors.lastname: Qu
authors.affiliation: Institute of Frontier Medical Science of Jilin University, Changchun 130021, P.R. China.
authors.forename: Chongyang
authors.initials: C
authors.lastname: Liang
mesh_terms: D000332:Aerobiosis / Q000502:physiology; D048429:Cell Size; D004271:DNA, Fungal / Q000235:genetics; D005285:Fermentation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D000432:Methanol / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D020411:Oligonucleotide Array Sequence Analysis; D020675:Peroxisomes / Q000378:metabolism; D010843:Pichia / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D012331:RNA, Fungal / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D012331:RNA, Fungal; D000432:Methanol