title: Internalization of the Extracellular Full-Length Tau Inside Neuro2A and Cortical Cells Is Enhanced by Phosphorylation.
abstract: Tau protein is mainly intracellular. However, several studies have demonstrated that full-length Tau can be released into the interstitial fluid of the brain. The physiological or pathological function of this extracellular Tau remains unknown. Moreover, as evidence suggests, extracellular Tau aggregates can be internalized by neurons, seeding Tau aggregation. However, much less is known about small species of Tau. In this study, we hypothesized that the status of phosphorylation could alter the internalization of recombinant Tau in Neuro2A and cortical cells. Our preliminary results revealed that the highly phosphorylated form of Tau entered the cells ten times more easily than a low phosphorylated one. This suggests that hyperphosphorylated Tau protein could spread between neurons in pathological conditions such as Alzheimer's disease.
authors.affiliation: Department of Neuroscience, Research Institute for Biosciences, University of Mons, Mons 7000, Belgium. mathilde.wauters@umons.ac.be.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Wauters
authors.affiliation: Department of Proteomics and Microbiology, Research Institute for Biosciences, University of Mons, Mons 7000, Belgium. ruddy.wattiez@umons.ac.be.
authors.forename: Ruddy
authors.initials: R
authors.lastname: Wattiez
authors.affiliation: Department of Neuroscience, Research Institute for Biosciences, University of Mons, Mons 7000, Belgium. laurence.ris@umons.ac.be.
authors.forename: Laurence
authors.initials: L
authors.lastname: Ris
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D010766:Phosphorylation; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000378:metabolism; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D016875:tau Proteins
keywords: Tau; internalization; phosphorylation; spreading