title: Polyacrylamide Gel-Entrapped Fungal Manganese Peroxidase from Ganoderma lucidum IBL-05 with Enhanced Catalytic, Stability, and Reusability Characteristics.
abstract: In the present study, polyacrylamide gel (PAG) was utilized as bolster material for the immobilization of in-house extracted and partially purified manganese peroxidase (MnP) through an entrapment technique yielding significant MnP immobilization (87.3±3.3 %) and remarkable stability of the enzyme (37.2±2.4 %) after a storage period of two months at 4°C. The immobilization also increased the optimal temperature by 10 °C and provided an alkaline shift of the pH optimum. Moreover, a significant enhancement in the thermo-stability was observed. After an incubation period of 72 h at 50°C, the PAG-entrapped-MnP still exhibited 41.2 % of the initial activity, whereas the free enzyme was completely inactive. Furthermore, PAG-entrapped-MnP showed an excellent recycling efficiency and retained more than 50% of its initial activity after five consecutive reaction cycles. In conclusion, owing to the economic feasibility, carrier-supported MnP may be a promising candidate for various applications in different industrial sectors.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Bilal
authors.forename: Muhammad
authors.initials: M
authors.lastname: Asgher
authors.affiliation: School of Engineering and Science, Tecnologico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N. L., CP 64849, Mexico. hafiz.iqbal@itesm.mx.
authors.forename: Hafiz M N
authors.initials: HM
authors.lastname: Iqbal
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000032:analysis*; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D020940:Reishi / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; C016680:polyacrylamide gels; D010544:Peroxidases; C051129:manganese peroxidase