title: Expression of BTBD7 in primary salivary adenoid cystic carcinoma and correlation with Slug and prognosis.
abstract: BACKGROUND
  BTB/POZ domain-containing protein 7 (BTBD7) is recognized as a regulatory gene that regulates epithelial cell dynamics and branching morphogenesis. It is also reported for regulating epithelial-mesenchymal transition (EMT) molecules and involved in the process of invasion and metastasis of lung cancer and hepatocellular carcinoma. Slug is a transcriptional factor of EMT which plays a crucial role in the process of primary salivary adenoid cystic carcinoma (SACC). However, the role of BTBD7 in SACC and the correlation with Slug have not been identified. This study investigated the expression of BTBD7 and correlation with Slug, as well as the prognostic significance of BTBD7 in SACC.
  
  
  METHODS
  The expression of BTBD7 and Slug were examined in ACC-LM and ACC-83 cell lines and immunohistochemically in paraffin embedded tissue specimens from 66 primary SACC patients. Statistical analyses were performed to evaluate the correlation between BTBD7 expression and Slug expression and the prognostic significance of BTBD7 expression.
  
  
  RESULTS
  BTBD7 protein expression was initially verified in ACC-LM and ACC-83 cell lines. The positive rate of BTBD7 expression was 62.1% in SACC to 20% in normal salivary tissues comparatively. BTBD7 expression was significantly correlated with Slug expression in SACC (P< 0.05). Increased BTBD7 expression was significantly associated with the TNM stage, tissue typing, distant metastasis and patients' poor clinical outcome.
  
  
  CONCLUSIONS
  Positive expression of BTBD7 in SACC could play an important role in the development of cancer and may serve as a favorable predictor for diagnosis and poor prognosis of patients.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong, China.|Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong, China.|Yantai Stomatological Hospital, Yantai, Shandong, China.|Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Yantai Stomatological Hospital, Yantai, Shandong, China.|Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong, China.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong, China.|Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Yantai Stomatological Hospital, Yantai, Shandong, China.
authors.forename: Zhonghao
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong, China.|Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong, China.
authors.forename: Xuxia
authors.initials: X
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D000368:Aged; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009367:Neoplasm Staging; D011379:Prognosis; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D000071250:Snail Family Transcription Factors / Q000235:genetics / Q000378:metabolism*; D047368:Tumor Burden
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C436517:BTBD7 protein, human; D009363:Neoplasm Proteins; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors
keywords: BTBD7; Slug; prognosis; salivary adenoid cystic carcinoma