title: Insight into the Kluyveromyces lactis Pdr1p regulon.
abstract: The overexpression of efflux pumps is an important mechanism leading to the development of multidrug resistance phenomenon. The transcription factor KlPdr1p, belonging to the Zn2Cys6family, is a central regulator of efflux pump expression in Kluyveromyces lactis. To better understand how KlPDR1-mediated drug resistance is achieved in K. lactis, we used DNA microarrays to identify genes whose expression was affected by deletion or overexpression of the KlPDR1 gene. Eighty-nine targets of the KlPDR1 were identified. From those the transcription of 16 genes was induced in the transformant overexpressing KlPDR1* and simultaneously repressed in the Klpdr1Δ deletion mutant. Almost all of these genes contain putative binding motifs for the AP-1-like transcription factors in their promoters. Furthermore, we studied the possible interplay between KlPdr1p and KlYap1p transcription factors. Our results show that KlYap1p does not significantly contribute to the regulation of KlPDR1 gene expression in the presence of azoles. However, KlPDR1 expression markedly increased in the presence of hydrogen peroxide and hinged upon the presence of KlYap1p. Our results show that although both KlPdr1p and KlYap1p transcription factors are involved in the control of K. lactis multidrug resistance, further studies will be needed to determine their interplay.
authors.affiliation: Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and Virology, Ilkovicova 6, Mlynska dolina, 842 15 Bratislava, Slovak Republic.|Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and Virology, Ilkovicova 6, Mlynska dolina, 842 15 Bratislava, Slovak Republic.
authors.forename: Nora
authors.initials: N
authors.lastname: Toth Hervay
authors.affiliation: Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and Virology, Ilkovicova 6, Mlynska dolina, 842 15 Bratislava, Slovak Republic.|Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and Virology, Ilkovicova 6, Mlynska dolina, 842 15 Bratislava, Slovak Republic.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Konecna
authors.affiliation: Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and Virology, Ilkovicova 6, Mlynska dolina, 842 15 Bratislava, Slovak Republic.|Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and Virology, Ilkovicova 6, Mlynska dolina, 842 15 Bratislava, Slovak Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Balazfyova
authors.affiliation: Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and Virology, Ilkovicova 6, Mlynska dolina, 842 15 Bratislava, Slovak Republic.|Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and Virology, Ilkovicova 6, Mlynska dolina, 842 15 Bratislava, Slovak Republic.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Svrbicka
authors.affiliation: Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and Virology, Ilkovicova 6, Mlynska dolina, 842 15 Bratislava, Slovak Republic.|Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and Virology, Ilkovicova 6, Mlynska dolina, 842 15 Bratislava, Slovak Republic.
authors.forename: Yvetta
authors.initials: Y
authors.lastname: Gbelska
mesh_terms: D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007716:Kluyveromyces / Q000737:chemistry / Q000235:genetics*; D016877:Oxidants / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D018085:Regulon / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D016877:Oxidants; D014157:Transcription Factors; D006861:Hydrogen Peroxide
keywords: DNA microarray; KlPDR1; KlYAP1; Kluyveromyces lactis; multidrug resistance; multirésistance; puce à ADN