title: [Effects and related mechanism of quercetin on thrombin-induced proliferation and migration of rat vascular smooth muscle cells].
abstract: OBJECTIVE
  To investigate the effects and related mechanism of quercetin on thrombin-induced proliferation and migration of rat vascular smooth muscle cells(VSMCs).
  
  
  METHODS
  Third to fifth generation VSMCs were divided into three groups, including control group (with PBS on the base of medium DMEM), thrombin group (with 1 U/ml thrombin on the base of medium DMEM) and quercetin group (treated with 100 μmol/L quercetin before 1 U/ml thrombin on the base of medium DMEM). The proliferation and migration capacitities were tested by CCK-8 kit and transwell chamber, respectively. The protein level of phospho-extracellular signal regulated kinase 1/2 and phosphor-p38 were measured by Western blot.
  
  
  RESULTS
  (1) According to the CCK-8 results, optical density value was significantly higher in thrombin group than that of control group(2.59±0.16 vs. 1.97±0.18, P<0.01), which could be significantly attenuated by pretreatment with quercetin(2.13±0.19, P<0.01), and there was no significant difference between quercetin group and control group(P>0.05). (2) The transwell results showed that the migrated VSMCs were significantly higher in thrombin group than in control group (1 337±162 vs. 99±26, P<0.01), which could be significantly reduced by pretreatment with quercetin (926±111, P<0.05), but still significantly higher than control group (P<0.01). (3) The protein expressions of phospho-extracellular signal regulated kinase 1/2 and phosphor-p38 were significantly upregulated in thrombin group compared to control group (both P<0.05), which could be significantly downregulated by pretreatment with quercetin (P<0.05).
  
  
  CONCLUSIONS
  Quercetin can effectively attenuate thrombin-induced vascular smooth muscle cells proliferation and migration, possibly through inhibiting the phosphorylation of extracellular signal regulated kinase 1/2 and p38 pathway.
authors.affiliation: Cardiovascular Research Institute, Three Gorges University, Yichang 443000, China.
authors.forename: Y R
authors.initials: YR
authors.lastname: Jiang
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: X W
authors.initials: XW
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Teng
authors.forename: J W
authors.initials: JW
authors.lastname: Ding
authors.forename: Q T
authors.initials: QT
authors.lastname: Zeng
authors.forename: J
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D010766:Phosphorylation; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013917:Thrombin / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D011794:Quercetin; D048052:Mitogen-Activated Protein Kinase 3; D048051:p38 Mitogen-Activated Protein Kinases; D013917:Thrombin