title: [Bioinformatics analysis of microRNA comprehensive regulatory network in B- cell acute lymphoblastic leukemia].
abstract: OBJECTIVE
  To reveal the involvement of molecules in the pathogenesis of B-cell acute lymphoblastic leukemia (B-ALL) by bioinformatics analyses.
  
  
  METHODS
  The microarray data of B-ALL were downloaded from the Gene Expression Omnibus (GEO) database and Qlucore Omics Explorer software was used to screen differentially expressed miRNA. Based on the differentially expressed miRNAs, we predicted the target genes, long non-coding RNAs (lncRNA) and transcription factors (TFs). Then we constructed the miRNAs-centered comprehensive regulatory network. In addition, we performed functional enrichment analysis to analyze the functions of target genes.
  
  
  RESULTS
  Of all the 15 differentially expressed miRNAs, 7 miRNAs were of overexpression, 8 miRNAs underexpressed. From the miRNAs comprehensive regulatory network, we found that hsa-miR-486-3p and hsa-miR-126 regulated a large number of target genes, hsa-miR-126 including target genes MYC. The hsa-miR-29a, hsa-miR-130a and hsa-miR-181c regu- lated a lot of lncRNAs containing X-inactive-specific transcript (XIST). The hsa-miR-181a-2, hsa-miR- 181b-2 and hsa-miR-663 were regulated by a host of TFs including caudal- related homeobox transcription fact2 (CDX2). Additionally, the target genes of has-miR-126 were enriched in Wnt pathways.
  
  
  CONCLUSIONS
  The expression of hsa-miR-29a , hsa-miR-126 and has-miR-181 family were significantly different in B-ALL. Target gene of MYC, TFs of CDX2 and lncRNA of XIST may play important roles in the development of B-ALL, serving as a potential therapeutic target.
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China.
authors.forename: M N
authors.initials: MN
authors.lastname: Zeng
authors.forename: W L
authors.initials: WL
authors.lastname: Ma
authors.forename: W L
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D019295:Computational Biology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
chemical_list: C527111:MIRN126 microRNA, human; C532045:MIRN29a microRNA, human; C560945:MIRN486 microRNA, human; C552243:MIRN663 microRNA, human; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D014157:Transcription Factors