title: Proteomic and morphological changes produced by subinhibitory concentration of isoniazid in Mycobacterium tuberculosis.
abstract: To study the proteomic and morphological changes in Mycobacterium tuberculosis H37Rv exposed to subinhibitory concentration of isoniazid (INH).
  
  
  
  The bacillus was exposed to ½ MIC of INH at 12, 24 and 48 h. The samples' cells were submitted to scanning electron microscopy. The proteins were separated by 2D gel electrophoresis and identified by MS.
  
  
  
  INH exposure was able to alter the format, the multiplication and causing a cell swelling in the bacillus. The major altered proteins were related to the virulence, detoxification, adaptation, intermediary metabolism and lipid metabolism.
  
  
  
  The protein and morphological changes in M. tuberculosis induced by ½ MIC INH were related to defense mechanism of the bacillus or the action of INH therein.
authors.affiliation: Department of Clinical Analyses & Biomedicine, State University of Maringá, Avenida Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Paula Az
authors.initials: PA
authors.lastname: Campanerut-Sá
authors.affiliation: Department of Clinical Analyses & Biomedicine, State University of Maringá, Avenida Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Luciana D
authors.initials: LD
authors.lastname: Ghiraldi-Lopes
authors.affiliation: Department of Clinical Analyses & Biomedicine, State University of Maringá, Avenida Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Jean E
authors.initials: JE
authors.lastname: Meneguello
authors.affiliation: Department of Biological Sciences, Federal University of Parana - Sector Palotina, Rua Pioneiro, 2153, 85950-000, Palotina, Paraná, Brazil.
authors.forename: Adriana
authors.initials: A
authors.lastname: Fiorini
authors.affiliation: Department of Biological Sciences, Federal University of Parana, Rua XV de Novembro, 1299, 80060-000, Curitiba, Paraná, Brazil.
authors.forename: Geisa Pc
authors.initials: GP
authors.lastname: Evaristo
authors.affiliation: Department of Clinical Analyses & Biomedicine, State University of Maringá, Avenida Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Vera Ld
authors.initials: VL
authors.lastname: Siqueira
authors.affiliation: Department of Clinical Analyses & Biomedicine, State University of Maringá, Avenida Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Regiane Bl
authors.initials: RB
authors.lastname: Scodro
authors.affiliation: Department of Clinical Analyses & Biomedicine, State University of Maringá, Avenida Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Eliana V
authors.initials: EV
authors.lastname: Patussi
authors.affiliation: Department of Biological Sciences, Federal University of Parana, Rua XV de Novembro, 1299, 80060-000, Curitiba, Paraná, Brazil.
authors.forename: Lucélia
authors.initials: L
authors.lastname: Donatti
authors.affiliation: Department of Biological Sciences, Federal University of Parana, Rua XV de Novembro, 1299, 80060-000, Curitiba, Paraná, Brazil.
authors.forename: Emanuel M
authors.initials: EM
authors.lastname: Souza
authors.affiliation: Department of Clinical Analyses & Biomedicine, State University of Maringá, Avenida Colombo, 5790, 87020-900, Maringá, Paraná, Brazil.
authors.forename: Rosilene F
authors.initials: RF
authors.lastname: Cardoso
mesh_terms: D000995:Antitubercular Agents / Q000032:analysis / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007538:Isoniazid / Q000032:analysis / Q000494:pharmacology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D040901:Proteomics; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; D007538:Isoniazid
keywords: 2D gel electrophoresis; MS; Mycobacterium tuberculosis; isoniazid; morphological changes; morphology; protein changes; proteome; scanning electron microscopy; tuberculosis