title: Distinct role of the Fas rs1800682 and FasL rs763110 polymorphisms in determining the risk of breast cancer among Han Chinese females.
abstract: BACKGROUND
  In recent years, studies have demonstrated that polymorphisms in the promoters of Fas and FasL are significantly associated with breast cancer risk. However, the results of these studies were inconsistent. This case-control study was performed to explore the associations between Fas rs1800682 and FasL rs763110 polymorphisms and breast cancer.
  
  
  METHODS
  A hospital-based case-control study of 560 Han Chinese females with breast cancer (583 controls) was conducted. The MassARRAY system was used to search for a possible association between the disease risk and the two single nucleotide polymorphisms, Fas rs1800682 and FasL rs763110. Statistical analyses were performed using SNPStats software to conduct Pearson's chi-square tests in five different genetic models. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated after adjustment to age and body mass index. PHASE v2.1 software was used to reconstruct all common haplotypes.
  
  
  RESULTS
  A statistically significant association was found between Fas rs1800682 and increased breast cancer risk (AG vs AA: OR =1.37, 95% CI =1.06-1.78; AA+AG vs GG: OR =1.32, 95% CI =1.04-1.66), and also it was found that the FasL rs763110 polymorphism may decrease the risk. Stratified analyses demonstrated that the rs763110 polymorphism was associated with lower breast cancer risk among postmenopausal females (heterozygote model: OR =0.69, 95% CI =0.49-0.97; dominant model: OR =0.70, 95% CI =0.51-0.96). The T allele of rs763110 was also associated with a decreased risk of lymph node metastasis (allele model: OR =0.75, 95% CI =0.57-0.97) and an increased risk of the breast cancer being human epidermal growth factor receptor 2 positive (allele model: OR =1.37, 95% CI =1.03-1.18). Moreover, haplotype analysis showed that Ars1800682Trs763110 was associated to a statistically significant degree with lower risk of breast cancer (OR =0.70, 95% CI =0.53-0.91).
  
  
  CONCLUSIONS
  These data suggest that the presence of Fas rs1800683 is an important risk factor for breast cancer, whereas FasL rs763110 may exert a protective effect against the onset of breast cancer.
authors.affiliation: Department of Oncology, Second Affiliated Hospital of Xi'an Jiaotong University.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Medical Oncology, Xi'an Central Hospital.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Oncology, Second Affiliated Hospital of Xi'an Jiaotong University.
authors.forename: Xi-Jing
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University.
authors.forename: Tian-Bo
authors.initials: TB
authors.lastname: Jin
authors.affiliation: Department of Anesthesia, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of China.
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Dai
authors.affiliation: Department of Oncology, Second Affiliated Hospital of Xi'an Jiaotong University.
authors.forename: Hua-Feng
authors.initials: HF
authors.lastname: Kang
authors.affiliation: Department of Oncology, Second Affiliated Hospital of Xi'an Jiaotong University.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Guan
authors.affiliation: Department of Oncology, Second Affiliated Hospital of Xi'an Jiaotong University.
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Ma
authors.affiliation: Department of Oncology, Second Affiliated Hospital of Xi'an Jiaotong University.
authors.forename: Xing-Han
authors.initials: XH
authors.lastname: Liu
authors.affiliation: Department of Oncology, Second Affiliated Hospital of Xi'an Jiaotong University.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Dai
mesh_terms: D000483:Alleles; D001943:Breast Neoplasms / Q000737:chemistry / Q000235:genetics*; D016022:Case-Control Studies; D053222:Fas Ligand Protein / Q000737:chemistry* / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors
chemical_list: C505661:FASLG protein, human; D053222:Fas Ligand Protein
keywords: Fas; FasL; breast cancer; risk; single nucleotide polymorphism