title: The Impact of Handling and Storage of Human Fecal Material on Bacterial Activity.
abstract: Fecal material prepared from human stools is frequently used for the assessment of bacterial degradation of active pharmaceutical ingredients as relevant data are useful for evaluating the potential for colonic drug delivery. The impact of handling and storage of human fecal material on bacterial activity was assessed by evaluating the degradation characteristics of metronidazole and olsalazine. Multiple freeze (-70°C)-thaw cycles should be avoided. Incubation of frozen material for about 2 h in the anaerobic workstation ensures regeneration of the highest possible bacterial activity. Material could be stored at -70°C for at least 12 months.
authors.affiliation: Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Athens, Greece.
authors.forename: Eleni
authors.initials: E
authors.lastname: Karatza
authors.affiliation: Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Vertzoni
authors.affiliation: Chemical and Pharmaceutical Development, Bayer Pharma AG, Wuppertal, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Muenster
authors.affiliation: Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Athens, Greece. Electronic address: reppas@pharm.uoa.gr.
authors.forename: Christos
authors.initials: C
authors.lastname: Reppas
mesh_terms: D000328:Adult; D000636:Aminosalicylic Acids / Q000032:analysis / Q000378:metabolism*; D000890:Anti-Infective Agents / Q000032:analysis / Q000378:metabolism; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005615:Freezing; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000032:analysis / Q000378:metabolism*; D008875:Middle Aged; D013048:Specimen Handling / Q000379:methods* / Q000592:standards
chemical_list: D000636:Aminosalicylic Acids; D000890:Anti-Infective Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D008795:Metronidazole; C032587:olsalazine
keywords: bacterial activity; fecal material; handling; humans; metronidazole; olsalazine; storage