title: Radiation induces premature chromatid separation via the miR-142-3p/Bod1 pathway in carcinoma cells.
abstract: Radiation-induced genomic instability plays a vital role in carcinogenesis. Bod1 is required for proper chromosome biorientation, and Bod1 depletion increases premature chromatid separation. MiR-142-3p influences cell cycle progression and inhibits proliferation and invasion in cervical carcinoma cells. We found that radiation induced premature chromatid separation and altered miR-142-3p and Bod1 expression in 786-O and A549 cells. Overexpression of miR-142-3p increased premature chromatid separation and G2/M cell cycle arrest in 786-O cells by suppressing Bod1 expression. We also found that either overexpression of miR-142-3p or knockdown of Bod1 sensitized 786-O and A549 cells to X-ray radiation. Overexpression of Bod1 inhibited radiation- and miR-142-3p-induced premature chromatid separation and increased resistance to radiation in 786-O and A549 cells. Taken together, these results suggest that radiation alters miR-142-3p and Bod1 expression in carcinoma cells, and thus contributes to early stages of radiation-induced genomic instability. Combining ionizing radiation with epigenetic regulation may help improve cancer therapies.
authors.affiliation: Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences & Key Laboratory of Space Radiobiology of Gansu Province, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Pan
authors.affiliation: Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences & Key Laboratory of Space Radiobiology of Gansu Province, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
authors.forename: Yarong
authors.initials: Y
authors.lastname: Du
authors.affiliation: Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences & Key Laboratory of Space Radiobiology of Gansu Province, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Ren
authors.affiliation: Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences & Key Laboratory of Space Radiobiology of Gansu Province, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
authors.forename: Yaxiong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences & Key Laboratory of Space Radiobiology of Gansu Province, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences & Key Laboratory of Space Radiobiology of Gansu Province, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
authors.forename: Jufang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences & Key Laboratory of Space Radiobiology of Gansu Province, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.|Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions and School for Radiological and Interdisciplinary Sciences (RAD-X), Soochow University, Suzhou, Jiangsu, China.
authors.forename: Burong
authors.initials: B
authors.lastname: Hu
mesh_terms: D002277:Carcinoma / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics* / Q000528:radiation effects; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002842:Chromatids / Q000235:genetics*; D020090:Chromosome Segregation / Q000235:genetics* / Q000528:radiation effects; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D042822:Genomic Instability; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009381:Neoplasms, Radiation-Induced / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D011837:Radiation-Protective Agents; D014407:Tumor Cells, Cultured; D014965:X-Rays / Q000009:adverse effects
chemical_list: C524692:Bod1 protein, human; D018797:Cell Cycle Proteins; C555159:MIRN142 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D011837:Radiation-Protective Agents
keywords: Bod1; miR-142-3p; premature chromatid separation; radiation; radiosensitivity