title: Novel A20-gene-eluting stent inhibits carotid artery restenosis in a porcine model.
abstract: BACKGROUND
  Carotid artery stenosis is a major risk factor for ischemic stroke. Although carotid angioplasty and stenting using an embolic protection device has been introduced as a less invasive carotid revascularization approach, in-stent restenosis limits its long-term efficacy and safety. The objective of this study was to test the anti-restenosis effects of local stent-mediated delivery of the A20 gene in a porcine carotid artery model.
  
  
  METHODS
  The pCDNA3.1EHA20 was firmly attached onto stents that had been collagen coated and treated with N-succinimidyl-3-(2-pyridyldithiol)propionate solution and anti-DNA immunoglobulin fixation. Anti-restenosis effects of modified vs control (the bare-metal stent and pCDNA3.1 void vector) stents were assessed by Western blot and scanning electron microscopy, as well as by morphological and inflammatory reaction analyses.
  
  
  RESULTS
  Stent-delivered A20 gene was locally expressed in porcine carotids in association with significantly greater extent of re-endothelialization at day 14 and of neointimal hyperplasia inhibition at 3 months than stenting without A20 gene expression.
  
  
  CONCLUSIONS
  The A20-gene-eluting stent inhibits neointimal hyperplasia while promoting re-endothelialization and therefore constitutes a novel potential alternative to prevent restenosis while minimizing complications.
authors.affiliation: Department of Neurology, Cerebrovascular Disease Research Institute, Southwest Hospital.
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Zhou
authors.affiliation: Department of Neurology, Cerebrovascular Disease Research Institute, Southwest Hospital.
authors.forename: Jing
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Neurology, Cerebrovascular Disease Research Institute, Southwest Hospital.
authors.forename: Zhao-You
authors.initials: ZY
authors.lastname: Meng
authors.affiliation: Department of Neurology, Cerebrovascular Disease Research Institute, Southwest Hospital.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Neurology, Cerebrovascular Disease Research Institute, Southwest Hospital.
authors.forename: Jia-Lu
authors.initials: JL
authors.lastname: Huang
authors.affiliation: Department of Neurology, Cerebrovascular Disease Research Institute, Southwest Hospital.
authors.forename: He-Qing
authors.initials: HQ
authors.lastname: Huang
authors.affiliation: Department of Anatomy, Key Laboratory for Biomechanics of Chongqing, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Anatomy, Key Laboratory for Biomechanics of Chongqing, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zeng
authors.affiliation: Department of Anatomy, Key Laboratory for Biomechanics of Chongqing, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Anatomy, Key Laboratory for Biomechanics of Chongqing, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Chu-Hong
authors.initials: CH
authors.lastname: Zhu
authors.affiliation: Department of Neurology, Cerebrovascular Disease Research Institute, Southwest Hospital.
authors.forename: Kang-Ning
authors.initials: KN
authors.lastname: Chen
mesh_terms: D000818:Animals; D023903:Coronary Restenosis / Q000517:prevention & control / Q000628:therapy*; D004195:Disease Models, Animal; D054855:Drug-Eluting Stents; D013552:Swine
keywords: A20; endothelialization; gene therapy; restenosis; stent