title: A simple aptamer-based fluorescent assay for the detection of Aflatoxin B1 in infant rice cereal.
abstract: A fluorescent assay for the rapid, sensitive and specific detection of Aflatoxin B1 (AFB1) was developed in this study. Initially, a DNA/DNA duplex was formed between a fluorescein-labeled AFB1 aptamer and its partially complementary DNA strand containing a quencher moiety, resulting in fluorescence quenching due to the close proximity of fluorophore and quencher. Upon the addition of AFB1, an aptamer/AFB1 complex was generated to release the quencher-modified DNA strand, thus recovered the fluorescence of fluorescein and enabled quantitative detection for AFB1 by monitoring fluorescence enhancement. Under optimized conditions, this assay exhibited a linear response to AFB1 in the range of 5-100ng/mL with a detection limit down to 1.6ng/mL. Trials of this assay in infant rice cereal with satisfactory recovery in the range of 93.0%-106.8%, demonstrate that the new assay could be a potential sensing platform for AFB1 determination in food.
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; College of Animal Science, Xinjiang Agricultural University, Urumchi 830052, PR China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wen
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Guo
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China.
authors.forename: Songli
authors.initials: S
authors.lastname: Li
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: zhengnan_1980@126.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Ministry of Agriculture - Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, PR China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: jiaqiwang@vip.163.com.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D052157:Aptamers, Nucleotide; D002523:Edible Grain / Q000737:chemistry; D005456:Fluorescent Dyes; D005506:Food Contamination / Q000032:analysis*; D007225:Infant Food / Q000032:analysis*; D057230:Limit of Detection; D012275:Oryza / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D016604:Aflatoxin B1
keywords: Aflatoxin B(1); Aptamer; Fluorescent; Infant rice cereal; Quenching