title: Prognostic Role of the Expression of Invasion-Related Molecules in Glioblastoma.
abstract: BackgroundGlioblastoma multiforme (GBM) is the most common malignant disease of the central nervous system. Its prognosis is unfavorable, and the median overall survival of patients is 16 to 24 months. The main cause of the poor survival data are the extensive invasion of cancer cells to the neighboring parenchyma, thus leading to inevitable local recurrence. The extracellular matrix (ECM) is a known factor in tumor invasion, and differences in the ECM of nontumor brain and glioblastoma has been proven.MethodsIn this research, 20 invasion-related expressions of ECM components were determined in 26 GBM flash-frozen samples using quantitative reverse transcription-polymerase chain reaction and proteomic measurements. Expression data were then set against the survival data of the patients.ResultsSignificant alterations between groups with different survival rates could not be established in the individual evaluation of the expression level of the selected molecules. However, statistical analysis of the expression pattern of invasion-related molecules revealed a correlation with prognosis. The positive predictive values of the messenger RNA (mRNA) and the proteomic expression studies were 0.85 and 0.89, respectively. The receiver operation characteristic value was 0.775 for the mRNA expression data and 0.875 for the protein expression data. Furthermore, a group of molecules, including brevican, cadherin-12, integrin β1, integrin α3, laminin α4, and laminin β1, that play a prominent role in invasion were identified.ConclusionsJoint assessment of the expression of invasion-related molecules provides a specific invasion spectrum of the tumor that correlates with the survival of glioblastoma patients. Using statistical classifiers enables the adoption of an invasion spectrum as a considerably accurate prognostic factor while gaining predictive information on potential molecular oncotherapeutic targets at the same time.
authors.affiliation: Department of Neurosurgery, University of Debrecen Clinical Center, Debrecen, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Virga
authors.affiliation: Department of Neurosurgery, University of Debrecen Clinical Center, Debrecen, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Bognár
authors.affiliation: Division of Neuropathology, Department of Pathology, University of Debrecen Clinical Center, Debrecen, Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Hortobágyi
authors.affiliation: Centre for Clinical Genomics and Personalized Medicine, University of Debrecen Clinical Center, Debrecen, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Zahuczky
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen Clinical Center Research, Debrecen, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Csősz
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen Clinical Center Research, Debrecen, Hungary.
authors.forename: Gergő
authors.initials: G
authors.lastname: Kalló
authors.affiliation: Department of Oncology, University of Debrecen Clinical Center, Debrecen, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Tóth
authors.affiliation: Department of Neurosurgery, University of Debrecen Clinical Center, Debrecen, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Hutóczki
authors.affiliation: Department of Neurosurgery, University of Debrecen Clinical Center, Debrecen, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Reményi-Puskár
authors.affiliation: Centre for Clinical Genomics and Personalized Medicine, University of Debrecen Clinical Center, Debrecen, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Steiner
authors.affiliation: Department of Neurosurgery, University of Debrecen Clinical Center, Debrecen, Hungary.
authors.forename: Almos
authors.initials: A
authors.lastname: Klekner
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D058581:Brevican / Q000378:metabolism; D000070585:Cadherin Related Proteins; D015820:Cadherins / Q000378:metabolism; D018572:Disease-Free Survival; D005109:Extracellular Matrix / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005909:Glioblastoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D006801:Humans; D039001:Integrin alpha Chains / Q000378:metabolism; D039641:Integrin beta Chains / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D015996:Survival Rate
chemical_list: D058581:Brevican; C442480:CDH23 protein, human; D000070585:Cadherin Related Proteins; D015820:Cadherins; D039001:Integrin alpha Chains; D039641:Integrin beta Chains; C501369:LAMA4 protein, human; C478221:LAMB1 protein, human; D007797:Laminin