title: Overexpression of the transmembrane carbonic anhydrase isoforms IX and XII in the inflamed synovium.
abstract: Juvenile idiopathic arthritis (JIA) is the most common form of chronic rheumatic disease affecting children worldwide, with some features similar to adult rheumatoid arthritis (RA). In the present study, we aim at investigating novel markers that will allow in the future for tailored, more personalized treatment strategies. Hence, taking notice of several reports proving the role of local acidosis as a causal link between inflammatory diseases and related pain, and the involvement of several carbonic anhydrases (CA, EC 4.2.1.1) isoforms in articular diseases, we evaluated in JIA patients the expression of these metalloenzymes. We identified that JIA patients show high levels of active CA IX and XII isoforms. Our results represent the first evidence of the identification of these enzymes as potential therapeutic targets and development of novel innovative therapies for arthritis, also considering that the two isoforms are validated antitumor targets.
authors.affiliation: a Department of Experimental and Clinical Biomedical Sciences , University of Florence , Florence , Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Margheri
authors.affiliation: b Neurofarba Department, University of Florence , Florence , Italy.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Ceruso
authors.affiliation: b Neurofarba Department, University of Florence , Florence , Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Carta
authors.affiliation: a Department of Experimental and Clinical Biomedical Sciences , University of Florence , Florence , Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Laurenzana
authors.affiliation: c Department of Experimental and Clinical Medicine , University of Florence , Florence , Italy , and.
authors.forename: Laura
authors.initials: L
authors.lastname: Maggi
authors.affiliation: d Anna Meyer Children's Hospital and University of Florence , Florence , Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Lazzeri
authors.affiliation: b Neurofarba Department, University of Florence , Florence , Italy.|d Anna Meyer Children's Hospital and University of Florence , Florence , Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Simonini
authors.affiliation: c Department of Experimental and Clinical Medicine , University of Florence , Florence , Italy , and.
authors.forename: Francesco
authors.initials: F
authors.lastname: Annunziato
authors.affiliation: a Department of Experimental and Clinical Biomedical Sciences , University of Florence , Florence , Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Del Rosso
authors.affiliation: b Neurofarba Department, University of Florence , Florence , Italy.
authors.forename: Claudiu T
authors.initials: CT
authors.lastname: Supuran
authors.affiliation: b Neurofarba Department, University of Florence , Florence , Italy.|d Anna Meyer Children's Hospital and University of Florence , Florence , Italy.
authors.forename: Rolando
authors.initials: R
authors.lastname: Cimaz
mesh_terms: D000293:Adolescent; D001171:Arthritis, Juvenile / Q000097:blood / Q000201:enzymology* / Q000378:metabolism; D000071231:Carbonic Anhydrase IX / Q000235:genetics* / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000235:genetics* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D006801:Humans; D015394:Molecular Structure; D013583:Synovial Membrane / Q000201:enzymology* / Q000378:metabolism
chemical_list: D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases; C477378:carbonic anhydrase XII
keywords: Carbonic anhydrase; juvenile idiopathic arthritis; synovium