title: Identification of Enterobacteriaceae and detection of carbapenemases from positive blood cultures by combination of MALDI-TOF MS and Carba NP performed after four hour subculture in Mueller Hinton.
abstract: A new protocol for Enterobacteriaceae identification and detection of carbapenemase-producing isolates from blood cultures by combining MALDI-TOF MS and the Carba NP test has been evaluated. Bacterial identification was correct in 129 of the 130 isolates tested while the Carba NP detected 28 out of the 29 carbapenemase producers.
authors.affiliation: Departamento de Biología Funcional, Área de Microbiología, Universidad de Oviedo, Spain; Servicio de Microbiología, Hospital Universitario Central de Asturias, Spain. Electronic address: javifdom@gmail.com.
authors.forename: Javier
authors.initials: J
authors.lastname: Fernández
authors.affiliation: Servicio de Microbiología, Hospital Universitario Central de Asturias, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Rodríguez-Lucas
authors.affiliation: Servicio de Microbiología, Hospital Universitario Central de Asturias, Spain.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Fernández-Suárez
authors.affiliation: Departamento de Biología Funcional, Área de Microbiología, Universidad de Oviedo, Spain; Servicio de Microbiología, Hospital Universitario Central de Asturias, Spain; Instituto Universitario Oftalmológico Fernández-Vega, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Vazquez
authors.affiliation: Departamento de Biología Funcional, Área de Microbiología, Universidad de Oviedo, Spain.
authors.forename: M Rosario
authors.initials: MR
authors.lastname: Rodicio
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000097:blood*; D001431:Bacteriological Techniques; D000071997:Blood Culture; D015780:Carbapenems / Q000494:pharmacology; D004755:Enterobacteriaceae / Q000145:classification* / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D001618:beta-Lactamases / Q000097:blood*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Blood cultures; Carba NP; Carbapenemase detection; MALDI-TOF MS