title: Ultraperformance convergence chromatography-high resolution tandem mass spectrometry for lipid biomarker profiling and identification.
abstract: Lipids represent biologically ubiquitous and highly dynamic molecules in terms of abundance and structural diversity. Whereas the potential for lipids to inform on disease/injury is promising, their unique characteristics make detection and identification of lipids from biological samples analytically demanding. We report the use of ultraperformance convergence chromatography (UPC2), a variant of supercritical fluid chromatography, coupled to high-resolution, data-independent tandem mass spectrometry for characterization of total lipid extracts from mouse lung tissue. The UPC2platform resulted in lipid class separation and when combined with orthogonal column chemistries yielded chromatographic separation of intra-class species based on acyl chain hydrophobicity. Moreover, the combined approach of using UPC2with orthogonal column chemistries, accurate mass measurements, time-aligned low- and high-collision energy total ion chromatograms, and positive and negative ion mode product ion spectra correlation allowed for confident lipid identification. Of great interest was the identification of differentially expressed ceramides that were elevated 24 h post whole thorax lung irradiation. The identification of lipids that were elevated 24 h post-irradiation signifies a unique opportunity to investigate early mechanisms of action prior to the onset of clinical symptoms in the whole thorax lung irradiation mouse model.
authors.affiliation: University of Maryland, School of Pharmacy, Department of Pharmaceutical Sciences, Baltimore, MD, USA.
authors.forename: Jace W
authors.initials: JW
authors.lastname: Jones
authors.affiliation: University of Maryland, School of Pharmacy, Department of Pharmaceutical Sciences, Baltimore, MD, USA.
authors.forename: Claire L
authors.initials: CL
authors.lastname: Carter
authors.affiliation: University of Maryland, School of Pharmacy, Department of Pharmaceutical Sciences, Baltimore, MD, USA.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: University of Maryland, School of Pharmacy, Department of Pharmaceutical Sciences, Baltimore, MD, USA.
authors.forename: Jianshi
authors.initials: J
authors.lastname: Yu
authors.affiliation: University of Maryland, School of Pharmacy, Department of Pharmaceutical Sciences, Baltimore, MD, USA.
authors.forename: Keely
authors.initials: K
authors.lastname: Pierzchalski
authors.affiliation: University of Maryland, School of Medicine, Department of Radiation Oncology, Baltimore, MD, USA.
authors.forename: Isabel L
authors.initials: IL
authors.lastname: Jackson
authors.affiliation: University of Maryland, School of Medicine, Department of Radiation Oncology, Baltimore, MD, USA.
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Vujaskovic
authors.affiliation: University of Maryland, School of Pharmacy, Department of Pharmaceutical Sciences, Baltimore, MD, USA.
authors.forename: Maureen A
authors.initials: MA
authors.lastname: Kane
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D002518:Ceramides / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid; D008055:Lipids / Q000032:analysis*; D051379:Mice; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015415:Biomarkers; D002518:Ceramides; D008055:Lipids
keywords: ceramides; convergence chromatography; lipidomics; lung irradiation; supercritical fluid chromatography