title: Mathematical Modeling of Cellular Metabolism.
abstract: Cellular metabolism basically consists of the conversion of chemical compounds taken up from the extracellular environment into energy (conserved in energy-rich bonds of organic phosphates) and a wide array of organic molecules serving as catalysts (enzymes), information carriers (nucleic acids), and building blocks for cellular structures such as membranes or ribosomes. Metabolic modeling aims at the construction of mathematical representations of the cellular metabolism that can be used to calculate the concentration of cellular molecules and the rates of their mutual chemical interconversion in response to varying external conditions as, for example, hormonal stimuli or supply of essential nutrients. Based on such calculations, it is possible to quantify complex cellular functions as cellular growth, detoxification of drugs and xenobiotic compounds or synthesis of exported molecules. Depending on the specific questions to metabolism addressed, the methodological expertise of the researcher, and available experimental information, different conceptual frameworks have been established, allowing the usage of computational methods to condense experimental information from various layers of organization into (self-) consistent models. Here, we briefly outline the main conceptual frameworks that are currently exploited in metabolism research.
authors.affiliation: Institute of Biochemistry-Computational Systems Biochemistry Group, Charité-Universitätsmedizin Berlin, 10117, Berlin, Germany.
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Berndt
authors.affiliation: Institute of Biochemistry-Computational Systems Biochemistry Group, Charité-Universitätsmedizin Berlin, 10117, Berlin, Germany. hergo@charite.de.
authors.forename: Hermann-Georg
authors.initials: HG
authors.lastname: Holzhütter
mesh_terms: D006801:Humans; D053858:Metabolic Networks and Pathways / Q000502:physiology*; D008954:Models, Biological; D008962:Models, Theoretical
keywords: Cellular metabolism; Kinetic model; Mathematical modeling; Statistical models; Stoichiometric models