title: Elevated mercury bound to serum proteins in methylmercury poisoned rats after selenium treatment.
abstract: Methylmercury is a toxic pollutant and is generated by microbial methylation of elemental or inorganic mercury in the environment. Previous study found decreased hepatic MDA levels and urinary mercury levels in methylmercury poisoned rats after sodium selenite treatment. This study further found increased mercury levels in serum samples from methylmercury poisoned rats after selenium treatment. By using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry, three Hg- binding protein fractions and two Se-binding protein fractions were identified with the molecular weight of approximately 21, 40, and 75 kDa and of 40 and 75 kDa, respectively. Elevated mercury level in the 75 kDa protein fraction was found binding with both Hg and Se, which may explain the decreased urinary Hg excretion in MeHg poisoned rats after Se treatment. MALDI-TOF-MS analysis of the serum found that the 75 kDa protein fractions were albumin binding with both Hg and Se and the 21 kDa fraction was Hg- binding metallothionein.
authors.affiliation: State Environmental Protection Engineering Center for Mercury Pollution Prevention and Control, and Laboratory of Metallomics and Nanometallomics, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shandong Maternity and Child Care Hospital, Jinan, 250001, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Fan
authors.affiliation: State Environmental Protection Engineering Center for Mercury Pollution Prevention and Control, and Laboratory of Metallomics and Nanometallomics, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Jiating
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Environmental Protection Engineering Center for Mercury Pollution Prevention and Control, and Laboratory of Metallomics and Nanometallomics, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Environmental Protection Engineering Center for Mercury Pollution Prevention and Control, and Laboratory of Metallomics and Nanometallomics, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jing
authors.affiliation: State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550002, China. shanglihai@vip.skleg.cn.
authors.forename: Lihai
authors.initials: L
authors.lastname: Shang
authors.affiliation: State Environmental Protection Engineering Center for Mercury Pollution Prevention and Control, and Laboratory of Metallomics and Nanometallomics, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Gao
authors.affiliation: State Environmental Protection Engineering Center for Mercury Pollution Prevention and Control, and Laboratory of Metallomics and Nanometallomics, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Bai
authors.initials: B
authors.lastname: Li
authors.affiliation: State Environmental Protection Engineering Center for Mercury Pollution Prevention and Control, and Laboratory of Metallomics and Nanometallomics, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China. liyf@ihep.ac.cn.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Li
mesh_terms: D000818:Animals; D001259:Ataxia / Q000097:blood / Q000188:drug therapy*; D001522:Behavior, Animal / Q000187:drug effects; D002417:Cattle; D008628:Mercury / Q000097:blood / Q000737:chemistry*; D008668:Metallothionein / Q000097:blood / Q000737:chemistry*; D008767:Methylmercury Compounds / Q000008:administration & dosage / Q000506:poisoning*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D008767:Methylmercury Compounds; D012710:Serum Albumin, Bovine; D008668:Metallothionein; D008628:Mercury; D012643:Selenium
keywords: Albumin; MALDI-TOF-MS; Methylmercury; Rats; SEC–ICP–MS; Selenite