title: Molecular cloning and functional analysis of the duck TIR domain-containing adaptor inducing IFN-β (TRIF) gene.
abstract: Toll-like receptors (TLRs) trigger the innate immune response by responding to specific components of microorganisms. The TIR domain-containing adaptor inducing IFN-β (TRIF) plays an essential role in mammalian TLR-mediated signaling. The role of TRIF in ducks (duTRIF) remains poorly understood. In this study, we cloned and characterized the full-length coding sequence of duTRIF from duck embryo fibroblasts (DEFs). In healthy ducks, duTRIF transcripts were broadly expressed in different tissues, with higher expression levels in the spleen and liver. Using quantitative real-time PCR (qRT-PCR), we demonstrated the upregulation of duTRIF in DEFs infected with AIV or DTMUV, and DEFs treated with Poly I:C or LPS. Overexpression of duTRIF was able to induce the NF-κB and IFN-β expression. Furthermore, the IFN induction function of duTRIF was impaired when Ala517 was mutated to Pro or His. Taken together, these results suggested that duTRIF regulated duck innate immune responses.
authors.affiliation: State Key Laboratory of Agriculture Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China; College of Agricultural and Animal Husbandry, Tibet University, Linzhi, 860000, PR China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Agriculture Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qian
authors.affiliation: College of Agricultural and Animal Husbandry, Tibet University, Linzhi, 860000, PR China.
authors.forename: Suolang
authors.initials: S
authors.lastname: Sizhu
authors.affiliation: State Key Laboratory of Agriculture Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Agriculture Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Meilin
authors.initials: M
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Agriculture Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, PR China. Electronic address: hbzhou@mail.hzau.edu.cn.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhou
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004372:Ducks / Q000276:immunology*; D005347:Fibroblasts / Q000276:immunology* / Q000821:virology; D007113:Immunity, Innate; D009980:Influenza A virus / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology*; D008099:Liver / Q000502:physiology; D016297:Mutagenesis, Site-Directed; D013154:Spleen / Q000502:physiology; D059467:Transcriptome
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D030161:Avian Proteins; C476286:Ticam1 protein, mouse
keywords: Duck; IFN-β; TRIF