title: Deciphering transcriptome profiles of peripheral blood mononuclear cells in response to PRRSV vaccination in pigs.
abstract: Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important viral diseases affecting swine industry worldwide. Despite routine farm vaccination, effective control strategies for PRRS remained elusive which underscores the need for in-depth studies to gain insight into the host immune response to vaccines. The current study aimed to investigate transcriptional responses to PRRS Virus (PRRSV) vaccine in the peripheral blood mononuclear cells (PBMCs) within 3 days following vaccination in German Landrace pigs.
  
  
  
  Transcriptome profiling of PBMCs from PRRSV vaccinated and age-matched unvaccinated pigs at right before (0 h), and at 6, 24 and 72 h after PRRSV vaccination was performed using the Affymetrix gene chip porcine gene 1.0 st array. Comparison of PBMCs transcriptome profiles between vaccinated and unvaccinated pigs revealed a distinct host innate immune transcriptional response to PRRSV vaccine. There was a significant temporal variation in transcriptional responses of PRRSV vaccine in PBMCs accounting 542, 2,263 and 357 differentially expressed genes (DEGs) at 6, 24 and 72 h post vaccination, respectively compared to the time point before vaccination (controls). Gene ontology analysis revealed the involvement of these DEGs in various biological process including innate immune response, signal transduction, positive regulation of MAP kinase activity, TRIF-dependent toll-like receptor signaling pathway, T cell differentiation and apoptosis. Immune response specific pathways such as cytokine-cytokine receptor interaction, chemokine signaling pathway, signal transduction, JAK-STAT pathway and regulation, TRAF6 mediated induction of NF-kB and MAPK, the NLRP3 inflammasome, endocytosis and interferon signaling were under regulation during the early stage of PRRSV vaccination. Network enrichment analysis revealed APP, TRAF6, PIN1, FOS, CTNNB1, TNFAIP3, TIP1, CDKN1, SIRT1, ESR1 and HDAC5 as the highly interconnected hubs of the functional network of PRRSV vaccine induced transcriptome changes in PBMCs.
  
  
  
  This study showed that a massive gene expression change occurred in PBMCs following PRRSV vaccination in German Landrace pigs. Within first 3 days of vaccine exposure, the highest transcript abundance was observed at 24 h after vaccination compared to that of control. Results of this study suggest that APP, TRAF6, PIN1, FOS, CDKN1A and TNFAIP3 could be considered as potential candidate genes for PRRSV vaccine responsiveness.
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany.|Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
authors.forename: Md Aminul
authors.initials: MA
authors.lastname: Islam
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Große-Brinkhaus
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany.
authors.forename: Maren Julia
authors.initials: MJ
authors.lastname: Pröll
authors.affiliation: Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
authors.forename: Muhammad Jasim
authors.initials: MJ
authors.lastname: Uddin
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany.
authors.forename: Sharmin Aqter
authors.initials: SA
authors.lastname: Rony
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany.
authors.forename: Dawit
authors.initials: D
authors.lastname: Tesfaye
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany.
authors.forename: Ernst
authors.initials: E
authors.lastname: Tholen
authors.affiliation: Teaching and Research Station on Frankenfrost, Faculty of Agriculture, University of Bonn, Königswinter, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hölker
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany.
authors.forename: Karl
authors.initials: K
authors.lastname: Schellander
authors.affiliation: Department of Animal Breeding and Husbandry, Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany. cneu@itw.uni-bonn.de.
authors.forename: Christiane
authors.initials: C
authors.lastname: Neuhoff
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D053263:Gene Regulatory Networks; D007113:Immunity, Innate; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D019318:Porcine Reproductive and Respiratory Syndrome / Q000517:prevention & control; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D013552:Swine; D013997:Time Factors; D059467:Transcriptome; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000906:Antibodies; D014765:Viral Vaccines; D012313:RNA
keywords: Antibody; Innate immunity; Microarray; PBMCs; PRRSV vaccine; Pig