title: Response Mechanisms in Serratia marcescens IBBPo15During Organic Solvents Exposure.
abstract: Serratia marcescens strain IBBPo15(KT315653) which possesses serratiopeptidase (ser) gene (KT894207) exhibited good solvent tolerance. During the exposure of S. marcescens IBBPo15cells to 5 % organic solvents, n-decane was less toxic for this bacterium, compared with n-hexane, cyclohexane, ethylbenzene, toluene, and styrene. The exposure of the S. marcescens IBBPo15cells to n-hexane, cyclohexane, ethylbenzene, toluene, and styrene induced the formation of large clusters, while in control and n-decane-exposed cells, only organization into small clusters was observed. The data obtained suggested that S. marcescens IBBPo15cells produced some secondary metabolites (i.e., surfactant serrawettin, red pigment prodigiosin) which are well known as valuable molecules due to their large applications. The exposure of the bacterial cells to organic solvents induced secondary metabolites profile modifications. However, S. marcescens IBBPo15possesses only alkB1, todM, rhlAB, pswP, mpr, and ser genes, the unspecific amplification of other fragments being acquired also when the primers for alkM1, xylM, ndoM, and C23DO genes were used. Modifications of DNA patterns were not depicted in S. marcescens IBBPo15cells exposed to organic solvents.
authors.affiliation: Institute of Biology Bucharest of Romanian Academy, 296 Splaiul Independentei, P.O. Box 56-53, 060031, Bucharest, Romania. mihaela.stancu@ibiol.ro.
authors.forename: Mihaela Marilena
authors.initials: MM
authors.lastname: Stancu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D009930:Organic Chemicals / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D012706:Serratia marcescens / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D009930:Organic Chemicals; D010447:Peptide Hydrolases; C022640:serratiopeptidase