title: Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles.
abstract: Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone resonance assignment of NS4B was obtained using conventional solution NMR experiments. Further studies suggested that NS4B contained eleven helices and six of them form five potential transmembrane regions. This study provides atomic level information for an important drug target to control flavivirus infections.
authors.affiliation: Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos, #03-01, Singapore, 138669, Singapore.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos, #03-01, Singapore, 138669, Singapore.
authors.forename: Ying Lei
authors.initials: YL
authors.lastname: Wong
authors.affiliation: Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos, #03-01, Singapore, 138669, Singapore.
authors.forename: Michelle Yueqi
authors.initials: MY
authors.lastname: Lee
authors.affiliation: Institute of Chemical & Engineering Sciences, Agency for Science, Technology, and Research, 1 Pesek Road, Jurong Island, Singapore, 627833, Singapore.
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Li
authors.affiliation: Novartis Institute for Tropical Diseases, Singapore, Singapore.
authors.forename: Qing-Yin
authors.initials: QY
authors.lastname: Wang
authors.affiliation: School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
authors.forename: Julien
authors.initials: J
authors.lastname: Lescar
authors.affiliation: Department of Biochemistry & Molecular Biology, Department of Pharmacology & Toxicology, Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX, USA.
authors.forename: Pei-Yong
authors.initials: PY
authors.lastname: Shi
authors.affiliation: Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos, #03-01, Singapore, 138669, Singapore. cbkang@etc.a-star.edu.sg.
authors.forename: CongBao
authors.identifier: 0000-0002-9886-9374
authors.initials: C
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000737:chemistry*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry*; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017361:Viral Nonstructural Proteins / Q000737:chemistry*
chemical_list: D008565:Membrane Proteins; D008823:Micelles; C000628254:NS4B protein, Dengue virus; D017361:Viral Nonstructural Proteins
keywords: NMR spectroscopy; NS4B; dengue virus; membrane protein; membrane topology