title: Conserved functions of human Pelota in mRNA quality control of nonstop mRNA.
abstract: Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. hPelota promoted the dissociation of stalled ribosomes at the 3' end of GFP-Rz mRNA, and mutations in domain A diminished this activity. The hPelota-R45A mutant associated with ribosomes but was defective in peptide release. Finally, hPelota promoted the degradation of GFP-Rz mRNA and suppressed the sequential endonucleolytic cleavages caused by stalled ribosomes at the 3' end of mRNA in dom34∆ mutant cells.
authors.affiliation: Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Ikeuchi
authors.affiliation: Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai, Japan.
authors.forename: Erina
authors.initials: E
authors.lastname: Yazaki
authors.affiliation: Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai, Japan.
authors.forename: Kazuhei
authors.initials: K
authors.lastname: Kudo
authors.affiliation: Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai, Japan. tinada@m.tohoku.ac.jp.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Inada
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics; D018388:Codon, Terminator / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D004720:Endonucleases; D004722:Endoribonucleases / Q000235:genetics; D055354:Exosomes / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D020125:Mutation, Missense; D059365:Nonsense Mediated mRNA Decay; D009687:Nuclear Proteins; D011485:Protein Binding; D000072417:Protein Domains; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; D018388:Codon, Terminator; D008840:Microfilament Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C112808:Dom34 protein, S cerevisiae; D004720:Endonucleases; D004722:Endoribonucleases; C550527:pelo protein, human
keywords: Pelota; mRNA quality control; nonstop decay; stalled ribosomes