title: Irisin immunostaining characteristics of breast and ovarian cancer cells.
abstract: To determine expression pattern of irisin in tissues obtained from human ovarian cancer, breast cancer, and cervix cancer. Tissue samples obtained from subjects with breast cancer, ovarian cancer cervix cancer, simple endometrial hyperplasia, complex atypical endometrial hyperplasia. At least five sections from each subject were immunohistochemically stained with irisin antibody, and H-score method was used to evaluate irisin intensity. Tissues obtained from healthy breast tissues, proliferative phase endometrium adenomyosis and benign ovarian tumors were accepted as control. Irisin activity was not detected in control breast tissues significantly increased irisin staining was detected in invasive lobular, intraductal papillary, invasive ductal, invasive papillary, and mucinous carcinomas compared to control tissues. Also, significantly increased irisin immunoreactivity was detected in both ovarian endometriosis and mucinous carcinomas compared to benign tumors. However irisin staining was not observed at the papillary carcinoma of the ovary while sections obtained from simple and complex atypical endometrial hyperplasia, and cervix carcinoma demonstrated irisin immunoreactivity. Increased irisin immunoreactivity in tissues obtained from breast, ovary, cervix carcinomas, and endometrial hyperplasia suggest critical role of this peptide during carcinogenesis.
authors.affiliation: Firat University School of Medicine, Department of Histology and Embryology Elazig Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Kuloglu
authors.affiliation: Private Clinic Obstetrics and Gynecology Usak Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Celik
authors.affiliation: Firat University School of Medicine, Department of Medical Biochemistry (Firat Hormones Research Group) Elazig Turkey saydin1@hotmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Aydin
authors.affiliation: Firat University School of Medicine, Department of Pathology Elazig Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Hanifi Ozercan
authors.affiliation: Istanbul Medipol University Hospital Department of Obstetrics and Gynecology Istanbul Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Acet
authors.affiliation: Ankara University School of Veterinary Medicine Ankara Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Aydin
authors.affiliation: Firat University School of Medicine, Department of Pathology Elazig Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Artas
authors.affiliation: Firat University School of Medicine, Department of Histology and Embryology Elazig Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Turk
authors.affiliation: Firat University School of Medicine, Department of Medical Biochemistry (Firat Hormones Research Group) Elazig Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Yardim
authors.affiliation: Firat University School of Veterinary Medicine, Department of Biochemistry Elazig Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Ozan
authors.affiliation: Firat University School of Veterinary Medicine, Department of Histology Elazig Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Hanifi Yalcin
authors.affiliation: Firat University School of Medicine, Department of Histology and Embryology Elazig Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Kocaman
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D004717:Endometrium / Q000378:metabolism / Q000473:pathology; D005260:Female; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: C577593:FNDC5 protein, human; D005353:Fibronectins