title: Oxidized (non)-regenerated cellulose affects fundamental cellular processes of wound healing.
abstract: In this study we investigated how hemostats such as oxidized regenerated cellulose (ORC, TABOTAMP) and oxidized non-regenerated cellulose (ONRC, RESORBA CELL) influence local cellular behavior and contraction of the extracellular matrix (ECM). Human stromal fibroblasts were inoculated in vitro with ORC and ONRC. Cell proliferation was assayed over time, and migration was evaluated by Live Cell imaging microscopy. Fibroblasts grown in collagen-gels were treated with ORC or ONRC, and ECM contraction was measured utilizing a contraction assay. An absolute pH decline was observed with both ORC and ONRC after 1 hour. Mean daily cell proliferation, migration and matrix contraction were more strongly inhibited by ONRC when compared with ORC (p < 0.05). When control media was pH-lowered to match the lower pH values typically seen with ORC and ONRC, significant differences in cell proliferation and migration were still observed between ONRC and ORC (p < 0.05). However, in these pH conditions, inhibition of matrix contraction was only significant for ONRC (p < 0.05). We find that ORC and ONRC inhibit fibroblast proliferation, migration and matrix contraction, and stronger inhibition of these essential cellular processes of wound healing were observed for ONRC when compared with ORC. These results will require further validation in future in vivo experiments to clarify the clinical implications for hemostat use in post-surgical wound healing.
authors.affiliation: Department of Vascular and Endovascular Surgery, University Hospital Düsseldorf, Moorenstraße 5, 40225 Düsseldorf, Germany.|Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford University, Stanford, CA 94305, USA.
authors.forename: M U
authors.initials: MU
authors.lastname: Wagenhäuser
authors.affiliation: Department of Vascular and Endovascular Surgery, University Hospital Düsseldorf, Moorenstraße 5, 40225 Düsseldorf, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Mulorz
authors.affiliation: Department of Vascular and Endovascular Surgery, University Hospital Düsseldorf, Moorenstraße 5, 40225 Düsseldorf, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Ibing
authors.affiliation: Department of Vascular and Endovascular Surgery, University Hospital Düsseldorf, Moorenstraße 5, 40225 Düsseldorf, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Simon
authors.affiliation: Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford University, Stanford, CA 94305, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Spin
authors.affiliation: Department of Vascular and Endovascular Surgery, University Hospital Düsseldorf, Moorenstraße 5, 40225 Düsseldorf, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schelzig
authors.affiliation: Department of Vascular and Endovascular Surgery, University Hospital Düsseldorf, Moorenstraße 5, 40225 Düsseldorf, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Oberhuber
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002483:Cellulose, Oxidized / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D002483:Cellulose, Oxidized