title: Measuring relative utilization of aerobic glycolysis in breast cancer cells by positional isotopic discrimination.
abstract: The ability of cancer cells to produce lactate through aerobic glycolysis is a hallmark of cancer. In this study, we established a positional isotopic labeling and LC-MS-based method that can specifically measure the conversion of glucose to lactate in glycolysis. We show that the rate of aerobic glycolysis is closely correlated with glucose uptake and lactate production in breast cancer cells. We also found that the production of [3-(13) C]lactate is significantly elevated in metastatic breast cancer cells and in early stage metastatic mammary tumors in mice. Our findings may enable the development of a biomarker for the diagnosis of aggressive breast cancer.
authors.affiliation: The Hormel Institute, University of Minnesota, Austin, MN, USA. dyang@hi.umn.edu, dyang@umn.edu.|The Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA. dyang@hi.umn.edu, dyang@umn.edu.
authors.forename: Da-Qing
authors.initials: DQ
authors.lastname: Yang
authors.affiliation: Department of Horticultural Science, University of Minnesota, Twin Cities, MN, USA.
authors.forename: Dana M
authors.initials: DM
authors.lastname: Freund
authors.affiliation: The Hormel Institute, University of Minnesota, Austin, MN, USA.
authors.forename: Benjamin R E
authors.initials: BR
authors.lastname: Harris
authors.affiliation: The Hormel Institute, University of Minnesota, Austin, MN, USA.
authors.forename: Defeng
authors.initials: D
authors.lastname: Wang
authors.affiliation: The Hormel Institute, University of Minnesota, Austin, MN, USA.|The Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.
authors.forename: Margot P
authors.initials: MP
authors.lastname: Cleary
authors.affiliation: Department of Horticultural Science, University of Minnesota, Twin Cities, MN, USA. hegem007@umn.edu.|Microbial and Plant Genomics Institute, University of Minnesota, Twin Cities, MN, USA. hegem007@umn.edu.|Department of Plant Biology, University of Minnesota, Twin Cities, MN, USA. hegem007@umn.edu.
authors.forename: Adrian D
authors.initials: AD
authors.lastname: Hegeman
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D006019:Glycolysis; D006801:Humans; D019344:Lactic Acid / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D019344:Lactic Acid
keywords: LC-MS; breast cancer and aerobic glycolysis; glucose uptake; stable isotopic labeling