title: Successful quantification of 4'-methyl-α-pyrrolidinohexanophenone (MPHP) in human urine using LC-TOF-MS in an autopsy case.
abstract: The toxicological detection of the new synthetic cathinone 4'-methyl-α-pyrrolidinohexanophenone (MPHP) in urine samples has been impossible, because much of MPHP is metabolized before its excretion into urine. In this study, we successfully quantified unmetabolized MPHP in urine of an autopsy case using a sensitive method by liquid chromatography-time-of-flight-mass spectrometry. The quantification method showed good linearity in the range of 1.00-100 ng/mL, and the limit of detection was 0.5 ng/mL in human urine. In the autopsy case, the concentrations of MPHP in urine, plasma, and liver tissue samples were determined to be 60.1, 32.9 ng/mL, and 63.1 ng/g, respectively.
authors.affiliation: Department of Forensic Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto, 602-8566 Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Shintani-Ishida
authors.affiliation: Department of Forensic Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto, 602-8566 Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kakiuchi
authors.affiliation: Department of Forensic Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto, 602-8566 Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikegaya
keywords: 4′-Methyl-α-pyrrolidinohexanophenone; Human urine; LC–TOF-MS; Liquid chromatography–time-of-flight-mass spectrometry; MPHP; Synthetic cathinone