title: Characterizing harmful advanced glycation end-products (AGEs) and ribosylated aggregates of yellow mustard seed phytocystatin: Effects of different monosaccharides.
abstract: Advanced glycation end products (AGEs) are at the core of variety of diseases ranging from diabetes to renal failure and hence gaining wide consideration. This study was aimed at characterizing the AGEs of phytocystatin isolated from mustard seeds (YMP) when incubated with different monosaccharides (glucose, ribose and mannose) using fluorescence, ultraviolet, circular dichroism (CD) spectroscopy and microscopy. Ribose was found to be the most potent glycating agent as evident by AGEs specific fluorescence and absorbance. YMP exists as a molten globule like structure on day 24 as depicted by high ANS fluorescence and altered intrinsic fluorescence. Glycated YMP as AGEs and ribose induced aggregates were observed at day 28 and 32 respectively. In our study we have also examined the anti-aggregative potential of polyphenol, resveratrol. Our results suggested the anti-aggregative behavior of resveratrol as it prevented the in vitro aggregation of YMP, although further studies are required to decode the mechanism by which resveratrol prevents the aggregation.
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Azaj
authors.initials: A
authors.lastname: Ahmed
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India.
authors.forename: Anas
authors.initials: A
authors.lastname: Shamsi
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India. Electronic address: bbbano17@gmail.com.
authors.forename: Bilqees
authors.initials: B
authors.lastname: Bano
mesh_terms: D052160:Benzothiazoles; D002942:Circular Dichroism; D020552:Comet Assay; D003224:Congo Red / Q000737:chemistry; D015891:Cystatins / Q000737:chemistry* / Q000648:ultrastructure; D017127:Glycation End Products, Advanced / Q000737:chemistry*; D006031:Glycosylation; D009005:Monosaccharides / Q000737:chemistry*; D009149:Mustard Plant / Q000737:chemistry*; D009391:Nephelometry and Turbidimetry; D010206:Papain / Q000037:antagonists & inhibitors; D066329:Protein Aggregates; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012266:Ribose / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013844:Thiazoles / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D015891:Cystatins; D017127:Glycation End Products, Advanced; D009005:Monosaccharides; D066329:Protein Aggregates; D013844:Thiazoles; C009462:thioflavin T; D003224:Congo Red; D012266:Ribose; D010206:Papain
keywords: AGEs; Aggregates; CD spectroscopy; Monosaccharides; Polyphenol; Resveratrol; Yellow mustard phytocystatin (YMP)