title: Nonlinear Optical Characterization of Membrane Protein Microcrystals and Nanocrystals.
abstract: Nonlinear optical methods such as second harmonic generation (SHG) and two-photon excited UV fluorescence (TPE-UVF) imaging are promising approaches to address bottlenecks in the membrane protein structure determination pipeline. The general principles of SHG and TPE-UVF are discussed here along with instrument design considerations. Comparisons to conventional methods in high throughput crystallization condition screening and crystal quality assessment prior to X-ray diffraction are also discussed.
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.
authors.forename: Justin A
authors.initials: JA
authors.lastname: Newman
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA. gsimpson@purdue.edu.
authors.forename: Garth J
authors.initials: GJ
authors.lastname: Simpson
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D004867:Equipment Design; D057166:High-Throughput Screening Assays; D006801:Humans; D007834:Lasers; D008565:Membrane Proteins / Q000737:chemistry*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods; D008859:Microscopy, Polarization / Q000295:instrumentation / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D012542:Scattering, Radiation; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D008565:Membrane Proteins
keywords: Crystal detection; Crystal optimisation; Crystal positioning; High-throughput screening; Microscopy; Nonlinear optical microscopy; Second harmonic generation; Two-photon excited UV fluorescence