title: Four-photon-excited fluorescence resonance energy transfer in an aqueous system from ZnSe:Mn/ZnS quantum dots to hypocrellin A.
abstract: In this work, we established a fluorescence resonance energy transfer (FRET) system between ZnSe:Mn/ZnS quantum dots and Hypocrellin A (HA, a photosensitizer used for photodynamic therapy of cancer) in aqueous solution, excited by four-photon. Here, the QDs are the donors and the HA are the acceptors. The four-photon-excited fluorescence resonance energy transfer spectrum was obtained under 1300nm femtosecond laser pluses. The experimental results indicated that the highest efficiency of FRET can reach up to 61.3%. Furthermore, the viability test in cancer cells was further demonstrated for biological applications of FRET system. When FRET occurs the cell killing rate of the cancer cells will reach to 84.8% with the 1mM concentration of HA. Our work demonstrates that while the four-photon excited FRET system is promising in both optics and biological applications, is also needs further investigation.
authors.forename: Yueshu
authors.initials: Y
authors.lastname: Feng
authors.forename: Liwei
authors.initials: L
authors.lastname: Liu
authors.forename: Siyi
authors.initials: S
authors.lastname: Hu
authors.forename: Yu
authors.initials: Y
authors.lastname: Ren
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Liu
authors.forename: Jingrui
authors.initials: J
authors.lastname: Xiu
authors.forename: Xihe
authors.initials: X
authors.lastname: Zhang
mesh_terms: D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D019800:Phenol; D017785:Photons; D045663:Quantum Dots; D011809:Quinones / Q000737:chemistry*; D018036:Selenium Compounds / Q000032:analysis*; D013440:Sulfides / Q000032:analysis*; D017967:Zinc Compounds / Q000032:analysis*
chemical_list: D011809:Quinones; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D019800:Phenol; D010569:Perylene; C031238:zinc sulfide; C044696:zinc selenide; C048388:hypocrellin A