title: Immune effect and safety evaluation of vaccine prepared by dendritic cells modified by rAAV-carrying BCSG1 gene.
abstract: The immune effect and safety evaluation of rAAV (recombinant adeno-associated virus)-containing Bcsg1 (breast cancer-specific gene 1) (rAAV/Bcsg1)-transfected DC (dendritic cell) (rAAV/Bcsg1-DC) vaccine in immunotherapy for (BCSG1) (+) BC was assessed. Immune effect of cytotoxic T lymphocytes (CTLs) on Bcsg1 (+) BC cells, and rAAV gene residuals in mature CTL cells and culture medium were determined. Nude mouse xenograft tumor model was established to assess the inhibition effects of DC-activated CTLs on tumor growth. DC cell surface markers were highly expressed in rAAV/Bcsg1 group and lysate-DC group, and rAAV/Bcsg1-DC-CTL showed stronger cytotoxic activity targeting Bcsg1 (+) BC cells. The rAAV/Bcsg1-DC vaccine-treated groups showed lower mean tumor weight, higher tumor inhibition rates and slower tumor growth. rAAV/Bcsg1-DC can induce highly efficient CTL-targeting Bcsg1 antigen without rAAV gene residuals.
authors.affiliation: Central Laboratory, Liaocheng People's Hospital, Liaocheng Clinical School of Taishan Medical University, Liaocheng, PRC.
authors.forename: W-H
authors.initials: WH
authors.lastname: Wang
authors.affiliation: Central Laboratory, Liaocheng People's Hospital, Liaocheng Clinical School of Taishan Medical University, Liaocheng, PRC.
authors.forename: C-H
authors.initials: CH
authors.lastname: Zhou
authors.affiliation: Central Laboratory, Liaocheng People's Hospital, Liaocheng Clinical School of Taishan Medical University, Liaocheng, PRC.
authors.forename: J
authors.initials: J
authors.lastname: Ding
authors.affiliation: Central Laboratory, Liaocheng People's Hospital, Liaocheng Clinical School of Taishan Medical University, Liaocheng, PRC.
authors.forename: Y-X
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: Central Laboratory, Liaocheng People's Hospital, Liaocheng Clinical School of Taishan Medical University, Liaocheng, PRC.
authors.forename: L-L
authors.initials: LL
authors.lastname: Zheng
authors.affiliation: Central Laboratory, Liaocheng People's Hospital, Liaocheng Clinical School of Taishan Medical University, Liaocheng, PRC.
authors.forename: S-F
authors.initials: SF
authors.lastname: Chen
authors.affiliation: Surgery of Mammary Gland and Thyroid, Liaocheng People's Hospital, Liaocheng Clinical School of Taishan Medical University, Liaocheng, PRC.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000009:adverse effects* / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology*; D000229:Dependovirus / Q000235:genetics; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000235:genetics* / Q000276:immunology; D009374:Neoplasms, Experimental / Q000276:immunology / Q000628:therapy; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D051845:gamma-Synuclein / Q000235:genetics* / Q000276:immunology
chemical_list: D019496:Cancer Vaccines; D009363:Neoplasm Proteins; C104249:SNCG protein, human; D051845:gamma-Synuclein