title: Performance Evaluation of the VIDAS(®) Measles IgG Assay and Its Diagnostic Value for Measuring IgG Antibody Avidity in Measles Virus Infection.
abstract: The objective of this study is primarily to compare the performance of the VIDAS(®) Measles immunoglobulin (Ig)G assay to that of two other serological assays using an immunoassay technique, Enzygnost(®) Anti-measles Virus/IgG (Siemens) and Measles IgG CAPTURE EIA(®) (Microimmune). The sensitivity and the agreement of the VIDAS(®) Measles IgG assay compared to the Enzygnost(®) Anti-measles Virus/IgG assay and the Measles IgG CAPTURE EIA(®) assay are 100%, 97.2% and 99.0%, 98.4%, respectively. The very low number of negative sera for IgG antibodies does not allow calculation of specificity. As a secondary objective, we have evaluated the ability of the VIDAS(®) Measles IgG assay to measure anti-measles virus IgG antibody avidity with the help of the VIDAS(®) CMV IgG Avidity reagent, using 76 sera from subjects with measles and 238 other sera. Different groups of populations were analyzed. In the primary infection measles group, the mean IgG avidity index was 0.16 (range of 0.07 to 0.93) compared to 0.79 (range of 0.25 to 1) in the serum group positive for IgG antibodies and negative for IgM. These data allow to define a weak anti-measles virus IgG antibody avidity as an avidity index (AI) < 0.3 and a strong avidity as an AI > 0.6. The VIDAS(®) Measles IgG assay has a performance equivalent to that of other available products. Its use, individual and quick, is well adapted to testing for anti-measles immunity in exposed subjects.
authors.affiliation: Department of Virology, CHU de Caen, F-14000 Caen, France. dina-j@chu-caen.fr.|Normandy University, Faculty of Medicine, UNICAEN, EA4655, F-14000 Caen, France. dina-j@chu-caen.fr.|National Reference Center (NRC) for Measles and Paramyxoviridae Respiratory Viruses, F-14000 Caen, France. dina-j@chu-caen.fr.
authors.forename: Julia
authors.initials: J
authors.lastname: Dina
authors.affiliation: Department of Biostatistics and Clinical Research, CHU de Caen, F-14000 Caen, France. creveuil-cr@chu-caen.fr.
authors.forename: Christian
authors.initials: C
authors.lastname: Creveuil
authors.affiliation: Department of Virology, CHU de Caen, F-14000 Caen, France. gouarin-s@chu-caen.fr.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Gouarin
authors.affiliation: Department of Virology, CHU de Caen, F-14000 Caen, France. viron-f@chu-caen.fr.
authors.forename: Florent
authors.initials: F
authors.lastname: Viron
authors.affiliation: Department of Virology, CHU de Caen, F-14000 Caen, France. hebert-am@chu-caen.fr.
authors.forename: Amelie
authors.initials: A
authors.lastname: Hebert
authors.affiliation: Department of Virology, CHU de Caen, F-14000 Caen, France. freymuth.francois@wanadoo.fr.
authors.forename: Francois
authors.initials: F
authors.lastname: Freymuth
authors.affiliation: Department of Virology, CHU de Caen, F-14000 Caen, France. vabret-a@chu-caen.fr.|Normandy University, Faculty of Medicine, UNICAEN, EA4655, F-14000 Caen, France. vabret-a@chu-caen.fr.|National Reference Center (NRC) for Measles and Paramyxoviridae Respiratory Viruses, F-14000 Caen, France. vabret-a@chu-caen.fr.
authors.forename: Astrid
authors.initials: A
authors.lastname: Vabret
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000097:blood*; D000915:Antibody Affinity; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood*; D007223:Infant; D008297:Male; D008457:Measles / Q000276:immunology*; D008459:Measles virus / Q000276:immunology*; D008875:Middle Aged; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G
keywords: measles avidity; measles diagnosis; measles virus