title: From bulk to plasmonic nanoparticle surfaces: the behavior of two potent therapeutic peptides, octreotide and pasireotide.
abstract: Octreotide and pasireotide are two cyclic somatostatin analogues with an important clinical use in the treatment and diagnosis of neuroendocrine tumors. Herein, by the combined use of several techniques (UV-visible absorption, fluorescence, circular dichroism, ζ-potential, transmission electron microscopy, Raman scattering, surface-enhanced Raman scattering, and quantum mechanical calculations) we have followed the structural dynamics of these analogues in the bulk, as well as their binding sites on plasmonic (gold and silver) colloids. In contrast to the previously derived conclusions, the two peptides seem to possess completely different conformational features. Octreotide, a cyclic octapeptide, is formed by a moderately flexible type-II'β-turn maintained by a deformable disulfide linkage. Pasireotide, in which the cyclic character is made possible by peptide bonds, manifests a rigid backbone formed by two oppositely placed tight turns of different types, i.e.γ-turn and type-I β-turn. Owing to their cationic character, both analogues induce aggregation of negatively charged gold and silver colloids. Nevertheless, despite their notable structural differences, both peptides bind onto gold nanoparticles through their unique d-Trp residue. In contrast, their binding to silver colloids seems to be of electrostatic nature, as formed through monodentate or bidentate ionic pairs.
authors.affiliation: Sorbonne Paris Cité, Université Paris 13, Groupe de Biophysique Moléculaire, UFR Santé-Médecine-Biologie Humaine, 74 Rue Marcel Cachin, 93017 Bobigny Cedex, France. mahmoud.ghomi@univ-paris13.fr.
authors.forename: Belén
authors.initials: B
authors.lastname: Hernández
authors.forename: Eduardo
authors.initials: E
authors.lastname: López-Tobar
authors.forename: Santiago
authors.initials: S
authors.lastname: Sanchez-Cortes
authors.forename: Yves-Marie
authors.initials: YM
authors.lastname: Coïc
authors.forename: Bruno
authors.initials: B
authors.lastname: Baron
authors.forename: Alexandre
authors.initials: A
authors.lastname: Chenal
authors.forename: Sergei G
authors.initials: SG
authors.lastname: Kruglik
authors.forename: Fernando
authors.initials: F
authors.lastname: Pflüger
authors.forename: Régis
authors.initials: R
authors.lastname: Cohen
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Ghomi