title: Modulators of Stomatal Lineage Signal Transduction Alter Membrane Contact Sites and Reveal Specialization among ERECTA Kinases.
abstract: Signal transduction from a cell's surface to its interior requires dedicated signaling elements and a cellular environment conducive to signal propagation. Plant development, defense, and homeostasis rely on plasma membrane receptor-like kinases to perceive endogenous and environmental signals, but little is known about their immediate downstream targets and signaling modifiers. Using genetics, biochemistry, and live-cell imaging, we show that the VAP-RELATED SUPPRESSOR OF TMM (VST) family is required for ERECTA-mediated signaling in growth and cell-fate determination and reveal a role for ERECTA-LIKE2 in modulating signaling by its sister kinases. We show that VSTs are peripheral plasma membrane proteins that can form complexes with integral ER-membrane proteins, thereby potentially influencing the organization of the membrane milieu to promote efficient and differential signaling from the ERECTA-family members to their downstream intracellular targets.
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305-5020, USA.
authors.forename: Chin-Min Kimmy
authors.initials: CM
authors.lastname: Ho
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305-5020, USA.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Paciorek
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305-5020, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Abrash
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305-5020, USA; HHMI, Stanford University, Stanford, CA 94305-5020, USA. Electronic address: dbergmann@stanford.edu.
authors.forename: Dominique C
authors.initials: DC
authors.lastname: Bergmann
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002450:Cell Communication / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D054046:Plant Stomata / Q000254:growth & development / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D050683:R-SNARE Proteins / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; C000601165:At3g60600 protein, Arabidopsis; D050683:R-SNARE Proteins; D011956:Receptors, Cell Surface; C457763:ER protein, Arabidopsis; C502169:ERL2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases