title: Tbx3 overexpression in human gastric cancer is correlated with advanced tumor stage and nodal status and promotes cancer cell growth and invasion.
abstract: The objective of the current study was to investigate the expression pattern of Tbx3 and its clinicopathological significance in patients with gastric cancer. The expression pattern of Tbx3 in gastric cancer tissues and adjacent noncancerous surface epithelia and mucosal glands was detected by immunohistochemistry. Tbx3 was found to be overexpressed in 46 of 98 human gastric cancer samples, and this correlated with advanced clinical stage, tumor stage, and nodal status. In addition, in the SGC-7901 gastric cancer cell line, Tbx3 overexpression by plasmid transfection promoted growth and invasion. Conversely, depleting Tbx3 expression by small-interfering RNA inhibited proliferation and invasion in BGC-823 cell line. Moreover, Tbx3 accelerated cell cycle progression at the G1/S boundary. Tbx3 also regulated epithelial-to-mesenchymal transition (EMT) to promote cell invasion by repressing E-cadherin expression and increasing expression levels N-cadherin, vimentin. These results indicate that in gastric cancer, Tbx3 plays an important role and might be a useful therapy target.
authors.affiliation: Department of Surgical Oncology, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Miao
authors.affiliation: Department of Surgical Oncology, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Xing-Yu
authors.initials: XY
authors.lastname: Liu
authors.affiliation: Department of Surgical Oncology, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Hui-Mian
authors.initials: HM
authors.lastname: Xu
authors.affiliation: Department of Surgical Oncology, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Zhen-Ning
authors.initials: ZN
authors.lastname: Wang
authors.affiliation: Department of Breast Surgery, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Zhao
authors.affiliation: Department of Surgical Oncology, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Yong-Xi
authors.initials: YX
authors.lastname: Song
authors.affiliation: Department of Surgical Oncology, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Xing
authors.affiliation: Department of Surgical Oncology, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Jin-Yu
authors.initials: JY
authors.lastname: Huang
authors.affiliation: Department of Surgical Oncology, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Jun-Yan
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: Department of Surgical Oncology, First Hospital of China Medical University, Shenyang, Liaoning Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Breast Surgery, First Hospital of China Medical University, Shenyang, Liaoning Province, China. yyxu_cmu@163.com.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D034741:RNA, Small Interfering / Q000235:genetics; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D020825:T-Box Domain Proteins / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D020825:T-Box Domain Proteins; C106884:TBX3 protein, human
keywords: Cell cycle; EMT; Gastric cancer; Tbx3