title: Molecular and biochemical evidence on the protective effects of quercetin in isoproterenol-induced acute myocardial injury in rats.
abstract: Cardioprotection represents one of the most important and realistic aspects of preventive therapy today. Quercetin, a naturally occurring dietary flavone, has been studied extensively for its antioxidant properties. The objective of present study is to find out the cardioprotective activity and to explore the underlying mechanisms of quercetin pretreatment (50 mg/kg body weight, orally) for 14 days against isoproterenol (ISO; 100 mg/kg body weight, subcutaneously) induced myocardial infarction in Wistar rats. Cardiac diagnostic markers, oxidative stress, inflammatory cytokines, histopathology along with gene expression analysis of calpain 1 and 2 were carried out in experimental rats. Quercetin pretreatment showed protective effects on heart by significantly attenuating the ISO-induced oxidative stress, inflammation, protecting heart architecture, and by downregulation of the expression of calpain. Overall, these findings revealed the cardio-protective potential of quercetin and its mechanism of action against ISO-induced MI in rats.
authors.affiliation: Department of Pharmacology & Toxicology, National Institute of Pharmaceutical Education and Research, Guwahati, 781 032, Assam, India.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Department of Pharmacology & Toxicology, National Institute of Pharmaceutical Education and Research, Guwahati, 781 032, Assam, India.
authors.forename: Eshvendar Reddy
authors.initials: ER
authors.lastname: Kasala
authors.affiliation: Department of Pharmacology & Toxicology, National Institute of Pharmaceutical Education and Research, Guwahati, 781 032, Assam, India.
authors.forename: Lakshmi Narendra
authors.initials: LN
authors.lastname: Bodduluru
authors.affiliation: Neuropharmacology Research Laboratory, Department of Pharmaceutics, Indian Institute of Technology (Banaras Hindu University), Varanasi, 221 005, Uttar Pradesh, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Pharmacology & Toxicology, National Institute of Pharmaceutical Education and Research, Guwahati, 781 032, Assam, India.|Department of Pharmacology, Gauhati Medical College, Guwahati, 781 032, India.
authors.forename: Mangala
authors.initials: M
authors.lastname: Lahkar
mesh_terms: D000818:Animals; D002154:Calpain / Q000096:biosynthesis; D002316:Cardiotonic Agents / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007545:Isoproterenol / Q000009:adverse effects / Q000494:pharmacology; D008297:Male; D009203:Myocardial Infarction / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002316:Cardiotonic Agents; D011794:Quercetin; D002154:Calpain; D007545:Isoproterenol
keywords: calpain; inflammatory cytokines; isoproterenol; myocardial infarction; quercetin; reactive oxygen species