title: Multi-omics profile of the mouse dentate gyrus after kainic acid-induced status epilepticus.
abstract: Temporal lobe epilepsy (TLE) can develop from alterations in hippocampal structure and circuit characteristics, and can be modeled in mice by administration of kainic acid (KA). Adult neurogenesis in the dentate gyrus (DG) contributes to hippocampal functions and has been reported to contribute to the development of TLE. Some of the phenotypical changes include neural stem and precursor cells (NPSC) apoptosis, shortly after their birth, before they produce hippocampal neurons. Here we explored these early phenotypical changes in the DG 3 days after a systemic injection of KA inducing status epilepticus (KA-SE), in mice. We performed a multi-omics experimental setup and analyzed DG tissue samples using proteomics, transcriptomics and microRNA profiling techniques, detecting the expression of 2327 proteins, 13401 mRNAs and 311 microRNAs. We here present a description of how these data were obtained and make them available for further analysis and validation. Our data may help to further identify and characterize molecular mechanisms involved in the alterations induced shortly after KA-SE in the mouse DG.
authors.affiliation: Neuroscience Program, Swammerdam Institute for Life Sciences, Faculty of Sciences, University of Amsterdam, Science Park 904, Amsterdam 1098XH, The Netherlands.
authors.forename: Marijn
authors.initials: M
authors.lastname: Schouten
authors.affiliation: Neuroscience Program, Swammerdam Institute for Life Sciences, Faculty of Sciences, University of Amsterdam, Science Park 904, Amsterdam 1098XH, The Netherlands.
authors.forename: Pascal
authors.initials: P
authors.lastname: Bielefeld
authors.affiliation: Oncoproteomics Laboratory, Cancer Center, Free University Amsterdam, De Boelelaan 1117, Amsterdam 1081HV, The Netherlands.
authors.forename: Silvina A
authors.initials: SA
authors.lastname: Fratantoni
authors.affiliation: Division of Pharmacology, LACDR, Leiden University, Einsteinweg 55, Leiden 2333 CC, The Netherlands.|Foundation of Epilepsy Institutes of The Netherlands (SEIN), Achterweg 5, Heemstede 2103 SW, The Netherlands.
authors.forename: Chantal J
authors.initials: CJ
authors.lastname: Hubens
authors.affiliation: Oncoproteomics Laboratory, Cancer Center, Free University Amsterdam, De Boelelaan 1117, Amsterdam 1081HV, The Netherlands.
authors.forename: Sander R
authors.initials: SR
authors.lastname: Piersma
authors.affiliation: Oncoproteomics Laboratory, Cancer Center, Free University Amsterdam, De Boelelaan 1117, Amsterdam 1081HV, The Netherlands.
authors.forename: Thang V
authors.initials: TV
authors.lastname: Pham
authors.affiliation: Division of Pharmacology, LACDR, Leiden University, Einsteinweg 55, Leiden 2333 CC, The Netherlands.|Foundation of Epilepsy Institutes of The Netherlands (SEIN), Achterweg 5, Heemstede 2103 SW, The Netherlands.
authors.forename: Rob A
authors.initials: RA
authors.lastname: Voskuyl
authors.affiliation: Neuroscience Program, Swammerdam Institute for Life Sciences, Faculty of Sciences, University of Amsterdam, Science Park 904, Amsterdam 1098XH, The Netherlands.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Lucassen
authors.affiliation: Oncoproteomics Laboratory, Cancer Center, Free University Amsterdam, De Boelelaan 1117, Amsterdam 1081HV, The Netherlands.
authors.forename: Connie R
authors.initials: CR
authors.lastname: Jimenez
authors.affiliation: Neuroscience Program, Swammerdam Institute for Life Sciences, Faculty of Sciences, University of Amsterdam, Science Park 904, Amsterdam 1098XH, The Netherlands.
authors.forename: Carlos P
authors.identifier: http://orcid.org/0000-0002-1556-2650
authors.initials: CP
authors.lastname: Fitzsimons
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000187:drug effects*; D004833:Epilepsy, Temporal Lobe / Q000139:chemically induced; D006624:Hippocampus / Q000187:drug effects; D007608:Kainic Acid; D051379:Mice; D013226:Status Epilepticus / Q000139:chemically induced
chemical_list: D007608:Kainic Acid