title: Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells.
abstract: To investigate the cellular effects of terahertz (THz) exposure, human corneal epithelial (HCE-T) cells derived from human eye were exposed to 0.12 THz radiation at 5 mW/cm² for 24 h, then the genotoxicity, morphological changes, and heat shock protein (Hsp) expression of the cells were examined. There was no statistically significant increase in the micronucleus (MN) frequency of cells exposed to 0.12 THz radiation compared with sham-exposed controls and incubator controls, whereas the MN frequency of cells treated with bleomycin for 1 h (positive control) did increase significantly. Similarly, there were no significant morphological changes in cells exposed to 0.12 THz radiation compared to sham-exposed controls and incubator controls, and Hsp expression (Hsp27, Hsp70, and Hsp90α) was also not significantly different between the three treatments. These results indicate that exposure to 0.12 THz radiation using the present conditions appears to have no or very little effect on MN formation, morphological changes, and Hsp expression in cells derived from human eye.
authors.affiliation: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. shin_koyama@rish.kyoto-u.ac.jp.
authors.forename: Shin
authors.initials: S
authors.lastname: Koyama
authors.affiliation: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. narita.eijirou.4e@kyoto-u.ac.jp.
authors.forename: Eijiro
authors.initials: E
authors.lastname: Narita
authors.affiliation: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. yoko_shimizu@rish.kyoto-u.ac.jp.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Shimizu
authors.affiliation: Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. shiina-takeo@ed.tmu.ac.jp.
authors.forename: Takeo
authors.initials: T
authors.lastname: Shiina
authors.affiliation: Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. masao@tmu.ac.jp.
authors.forename: Masao
authors.initials: M
authors.lastname: Taki
authors.affiliation: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. shino@rish.kyoto-u.ac.jp.
authors.forename: Naoki
authors.initials: N
authors.lastname: Shinohara
authors.affiliation: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. miyakoshi@rish.kyoto-u.ac.jp.
authors.forename: Junji
authors.initials: J
authors.lastname: Miyakoshi
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D004249:DNA Damage; D004574:Electromagnetic Fields / Q000009:adverse effects*; D004847:Epithelial Cells / Q000166:cytology*; D019573:Epithelium, Corneal / Q000166:cytology*; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015162:Micronucleus Tests; D018832:Molecular Chaperones; D013997:Time Factors
chemical_list: D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; C533585:HSP90AA2P protein, human; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones
keywords: cellular genotoxicity; heat shock protein (Hsp); human eye cells; long-term exposure; micronucleus (MN) formation; morphological changes; terahertz (THz)