title: Ultra-dense SNP genetic map construction and identification of SiDt gene controlling the determinate growth habit in Sesamum indicum L.
abstract: Sesame (Sesamum indicum L.) is an important oilseed crop and has an indeterminate growth habit. Here we resequenced the genomes of the parents and 120 progeny of an F2 population derived from crossing Yuzhi 11 (indeterminate, Dt) and Yuzhi DS899 (determinate, dt1), and constructed an ultra-dense SNP map for sesame comprised of 3,041 bins including 30,193 SNPs in 13 linkage groups (LGs) with an average marker density of 0.10 cM. Results indicated that the same recessive gene controls the determinacy trait in dt1 and a second determinate line, dt2 (08TP092). The QDt1 locus for the determinacy trait was located in the 18.0 cM-19.2 cM interval of LG8. The target SNP, SiDt27-1, and the determinacy gene, DS899s00170.023 (named here as SiDt), were identified in Scaffold 00170 of the Yuzhi 11 reference genome, based on genetic mapping and genomic association analysis. Unlike the G397A SNP change in the dt1 genotype, the SiDt allele in dt2 line was lost from the genome. This example of map-based gene cloning in sesame provides proof-of-concept of the utility of ultra-dense SNP maps for accurate genome research in sesame.
authors.affiliation: Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, People's Republic of China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, People's Republic of China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Miao
authors.affiliation: Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, People's Republic of China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, People's Republic of China.
authors.forename: Libin
authors.initials: L
authors.lastname: Wei
authors.affiliation: Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, People's Republic of China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, People's Republic of China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Kong
authors.affiliation: Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, People's Republic of China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Xu
authors.affiliation: Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, People's Republic of China.
authors.forename: Shuxian
authors.initials: S
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D020869:Gene Expression Profiling; D017343:Genes, Plant; D020641:Polymorphism, Single Nucleotide; D017386:Sequence Homology, Amino Acid; D031685:Sesamum / Q000235:genetics* / Q000254:growth & development*