title: Interleukin-18 Binding Protein Pretreatment Attenuates Kidney Injury Induced by Hepatic Ischemia Reperfusion.
abstract: Acute kidney injury (AKI) is a serious condition that can be induced by liver transplantation, major hepatic resection or prolonged portal vein occlusion. AKI can increase the frequency of postoperative complications. In the current study, we aimed to investigate whether interleukin-18 binding protein (IL-18BP) pretreatment has a protective effect against possible kidney injury following liver ischemia-reperfusion (IR) achieved by Pringle maneuver in an experimental rat model.
  
  
  
  A total of 24 male Wistar albino rats were included in this study. Animals were equally and randomly separated into 3 groups as follows: I, Sham group, II, IR group (1-hour ischemia and 4-hour reperfusion) and III, IR + IL-18BP group (50μg/kg IL-18BP was intraperitoneally administered 30 minutes before surgery). Blood, liver and kidney samples were collected for histopathological and biochemical (hepatic and renal function, nitric oxide, malondialdehyde and glutathione levels) analysis. In addition, proinflammatory cytokines including tumor necrosis factor α, IL-1β and IL-6 levels were measured in kidney tissues.
  
  
  
  IL-18BP has improved kidney functions in acute kidney damage, restored structural changes, exhibited anti-inflammatory effects by decreasing proinflammatory cytokines and regulated the oxidative stress parameters by antioxidant effect.
  
  
  
  Current study would be the first to evaluate the protective, antioxidant and anti-inflammatory effects of IL-18BP on renal damage induced by liver ischemia (1 hour) and reperfusion (4 hours). As a result, we have demonstrated that AKI may develop after hepatic IR with Pringle maneuver and IL-18BP pretreatment can attenuate this damage. By this way, complications related to liver IR could be minimized and also postoperative hospitalization durations, treatment costs and healing periods could be decreased.
authors.affiliation: Department of Anatomy, Afyon Kocatepe University, Afyon, Turkey. Electronic address: yucel94@hotmail.com.
authors.forename: Yucel
authors.initials: Y
authors.lastname: Gonul
authors.affiliation: Department of Anatomy, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Senem
authors.initials: S
authors.lastname: Kazandı
authors.affiliation: Department of Histology and Embryology, Dumlupınar University, Kütahya, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kocak
authors.affiliation: Department of Nephrology, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Ahsen
authors.affiliation: Department of General Surgery, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Bal
authors.affiliation: Department of Pediatric Surgery, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Afra
authors.initials: A
authors.lastname: Karavelioglu
authors.affiliation: Department of Biochemistry, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Hazman
authors.affiliation: Department of Anatomy, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Ozan
authors.initials: O
authors.lastname: Turamanlar
authors.affiliation: Department of Anesthesia and Reanimation, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Serdar
authors.initials: S
authors.lastname: Kokulu
authors.affiliation: Department of Nephrology, Afyon Kocatepe University, Afyon, Turkey.
authors.forename: Seref
authors.initials: S
authors.lastname: Yuksel
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage*; D008107:Liver Diseases / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy* / Q000473:pathology
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; C118370:interleukin-18 binding protein
keywords: Acute kidney injury; Inflammation; Interleukin-18 binding protein; Liver ischemia-reperfusion; Oxidative stress