title: The proteome of baker's yeast mitochondria.
abstract: In the past decade mass spectrometry-based proteomics has greatly contributed to shaping our knowledge about Saccharomyces cerevisiae mitochondria, from the initial identification of novel essential components in purified protein complexes, to the actual characterization of the mitochondrial proteome, the specific analysis of mitochondrial subcompartment proteomes, and the study of regulatory mechanisms that govern mitochondrial homeostasis. Here, we provide an overview of relevant mitochondrial proteome studies and furthermore discuss future possibilities how proteomics will further improve our existing understanding of mitochondria. Although mitochondria were the first organelles that have been intensively studied using proteomics, indeed the recent progress and development of more powerful and sensitive methods, instrumentation and data analysis strategies indicate that we are only beginning to exploit the full potential of mitochondrial proteomics and its possibilities to decipher cell biology. Beside mere (quantitative) inventory under different conditions, this will for instance include studying the role of multiple post-translational modifications in mitochondrial homeostasis as well as the system-wide mapping of protein-protein complexes and protein-lipid interactions.
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Dortmund, Germany; CAPES Foundation, Ministry of Education of Brazil, Caixa Postal 250, Brasília, DF, 70040-020, Brazil.
authors.forename: Humberto
authors.initials: H
authors.lastname: Gonczarowska-Jorge
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Dortmund, Germany.
authors.forename: René P
authors.initials: RP
authors.lastname: Zahedi
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Dortmund, Germany; Medizinisches Proteom-Center, Ruhr-University Bochum, Universitätsstr. 150, D-44801, Bochum, Germany; Department of Chemistry, College of Physical Sciences, University of Aberdeen, Aberdeen, Scotland, UK. Electronic address: albert.sickmann@isas.de.
authors.forename: Albert
authors.initials: A
authors.lastname: Sickmann
mesh_terms: D019295:Computational Biology; D013058:Mass Spectrometry; D008928:Mitochondria / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis*
chemical_list: D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins
keywords: Mass spectrometry; Mitochondrial Proteomics; Yeast