title: Proteomic and genomic analysis reveals novelCampylobacter jejuniouter membrane proteins and potential heterogeneity.
abstract: Gram-negative bacterial outer membrane proteins play important roles in the interaction of bacteria with their environment including nutrient acquisition, adhesion and invasion, and antibiotic resistance. In this study we identified 47 proteins within the Sarkosyl-insoluble fraction ofCampylobacter jejuni81-176, using LC-ESI-MS/MS. Comparative analysis of outer membrane protein sequences was visualised to reveal protein distribution within a panel ofCampylobacterspp., identifying severalC. jejuni-specific proteins. Smith-Waterman analyses ofC. jejunihomologues revealed high sequence conservation amongst a number of hypothetical proteins, sequence heterogeneity of other proteins and several proteins which are absent in a proportion of strains.
authors.affiliation: Moredun Research Institute, Bush Loan, Penicuik, United Kingdom.
authors.forename: Eleanor
authors.initials: E
authors.lastname: Watson
authors.affiliation: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom.
authors.forename: Aileen
authors.initials: A
authors.lastname: Sherry
authors.affiliation: Moredun Research Institute, Bush Loan, Penicuik, United Kingdom.
authors.forename: Neil F
authors.initials: NF
authors.lastname: Inglis
authors.affiliation: Moredun Research Institute, Bush Loan, Penicuik, United Kingdom.
authors.forename: Alex
authors.initials: A
authors.lastname: Lainson
authors.affiliation: Moredun Research Institute, Bush Loan, Penicuik, United Kingdom.
authors.forename: Dushyanth
authors.initials: D
authors.lastname: Jyothi
authors.affiliation: Moredun Research Institute, Bush Loan, Penicuik, United Kingdom.
authors.forename: Raja
authors.initials: R
authors.lastname: Yaga
authors.affiliation: Moredun Research Institute, Bush Loan, Penicuik, United Kingdom.
authors.forename: Erin
authors.initials: E
authors.lastname: Manson
authors.affiliation: Moredun Research Institute, Bush Loan, Penicuik, United Kingdom.
authors.forename: Lisa
authors.initials: L
authors.lastname: Imrie
authors.affiliation: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom.
authors.forename: Paul
authors.initials: P
authors.lastname: Everest
authors.affiliation: Moredun Research Institute, Bush Loan, Penicuik, United Kingdom; Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom.
authors.forename: David G E
authors.initials: DG
authors.lastname: Smith
keywords: Campylobacter jejuni; Genomics; Outer membrane; Proteomics