title: Rhizobium hidalgonense sp. nov., a nodule endophytic bacterium of Phaseolus vulgaris in acid soil.
abstract: One Gram-negative, aerobic, motile, rod-shaped bacterium, designated as FH14T, was isolated from nodules of Phaseolus vulgaris grown in Hidalgo State of Mexico. Results based upon 16S rRNA gene (≥99.8 % similarities to known species), concatenated sequence (recA, atpD and glnII) analysis of three housekeeping genes (≤93.4 % similarities to known species) and average nucleotide identity (ANI) values of genome sequence (ranged from 87.6 to 90.0 % to related species) indicated the distinct position of strain FH14Twithin the genus Rhizobium. In analyses of symbiotic genes, only nitrogen fixation gene nifH was amplified that had nucleotide sequence identical to those of the bean-nodulating strains in R. phaseoli and R. vallis, while nodulation gene nodC gene was not amplified. The failure of nodulation to its original host P. vulgaris and other legumes evidenced the loss of its nodulation capability. Strain FH14Tcontained summed feature 8 (C18:1ω6c/C18:1ω7c, 59.96 %), C16:0(10.6 %) and summed feature 2 (C12:0aldehyde/unknown 10.928, 10.24 %) as the major components of cellular fatty acids. Failure to utilize alaninamide, and utilizing L-alanine, L-asparagine and γ-amino butyric acid as carbon source, distinguished the strain FH14Tfrom the type strains for the related species. The genome size and DNA G+C content of FH14Twere 6.94 Mbp and 60.8 mol %, respectively. Based on those results, a novel specie in Rhizobium, named Rhizobium hidalgonense sp. nov., was proposed, with FH14T(=HAMBI 3636T= LMG 29288T) as the type strain.
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.affiliation: State Key Laboratory for Agro-Biotechnology, Beijing, 100193, People's Republic of China.|Rhizobium Research Center and College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.affiliation: State Key Laboratory for Agro-Biotechnology, Beijing, 100193, People's Republic of China.|Rhizobium Research Center and College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
authors.forename: Li Xue
authors.initials: LX
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Agro-Biotechnology, Beijing, 100193, People's Republic of China.|Rhizobium Research Center and College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
authors.forename: Wen Feng
authors.initials: WF
authors.lastname: Chen
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors.forename: Xiao Xia
authors.initials: XX
authors.lastname: Zhang
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340, Mexico DF, Mexico.
authors.forename: Myrthala M
authors.initials: MM
authors.lastname: Verástegui-Valdés
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340, Mexico DF, Mexico. entaowang@yahoo.com.mx.
authors.forename: En Tao
authors.initials: ET
authors.lastname: Wang
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, People's Republic of China. xzhan@iga.ac.cn.
authors.forename: Xiao Zeng
authors.initials: XZ
authors.lastname: Han
mesh_terms: D000409:Alanine / Q000378:metabolism; D001216:Asparagine / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D008800:Mexico; D008969:Molecular Sequence Data; D027805:Phaseolus / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012987:Soil; D001216:Asparagine; D000409:Alanine
keywords: Acid soil; México; Phaseolus vulgaris; Rhizobium