title: Chemical Constituents Identified from Fruit Body ofCordyceps bassianaand Their Anti-Inflammatory Activity.
abstract: Cordyceps bassianais one of Cordyceps species with anti-oxidative, anti-cancer, anti-inflammatory, anti-diabetic, anti-obesity, anti-angiogenic, and anti-nociceptive activities. This mushroom has recently demonstrated to have an ability to reduce 2,4-dinitrofluorobenzene-induced atopic dermatitis symptoms in NC/Nga mice. In this study, we further examined phytochemical properties of this mushroom by column chromatography and HPLC analysis. By chromatographic separation and spectroscopic analysis, 8 compounds, such as 1,9-dimethylguanine (1), adenosine (2), uridine (3), nicotinamide (4), 3-methyluracil (5), 1,7-dimethylxanthine (6), nudifloric acid (7), and mannitol (8) were identified from 6 different fractions and 4 more subfractions. Through evaluation of their anti-inflammatory activities using reporter gene assay and mRNA analysis, compound 1 was found to block luciferase activity induced by NF-κB and AP-1, suppress the mRNA levels of cyclooxygenase (COX)-2 and tumor necrosis factor (TNF)-α. Therefore, our data strongly suggests that compound 1 acts as one of major principles inCordyceps bassianawith anti-inflammatory and anti-atopic dermatitis activities.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
authors.forename: Wonse
authors.initials: W
authors.lastname: Suh
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.
authors.forename: Gyeongsug
authors.initials: G
authors.lastname: Nam
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.
authors.forename: Woo Seok
authors.initials: WS
authors.lastname: Yang
authors.affiliation: Institute for Bio-Medical Convergence, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University, Incheon 22711, Republic of Korea.
authors.forename: Gi-Ho
authors.initials: GH
authors.lastname: Sung
authors.affiliation: College of Pharmacy, Duksung Women's University, Seoul 01369, Republic of Korea.
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Shim
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.
authors.forename: Jae Youl
authors.initials: JY
authors.lastname: Cho
keywords: 1,9-dimethylguanine; AP-1; Anti-inflammatory activity; Cordyceps bassiana; NF-κB; Phytochemical study