title: Genetic polymorphisms affecting antioxidant enzymes are present in tympanosclerosis patients.
abstract: BACKGROUND
  This study investigated genetic polymorphisms affecting the inducible nitric oxide synthase, superoxide dismutase and catalase enzymes in chronic otitis media patients with and without tympanosclerosis, and the role of genetic susceptibility in the disease aetiology.
  
  
  METHODS
  A total of 162 patients who underwent surgery for chronic otitis media were divided into two study groups: a tympanosclerosis group and a chronic otitis media group. A third, the control, group comprised 188 healthy volunteers. Venous blood samples were evaluated using reverse transcriptase polymerase chain reaction.
  
  
  RESULTS
  There was a significant difference in GG genotype distribution of the -277A>G polymorphism in the NOS2 gene between the tympanosclerosis and control groups (p T) polymorphism in the SOD2 gene (p > 0.05). There were significant differences in the TT genotype distribution of the -21A>T polymorphism in the CAT gene between the tympanosclerosis and control groups, and between the chronic otitis media and control groups (p < 0.05).
  
  
  CONCLUSIONS
  These results suggest that genetic predisposition may play a role in the aetiopathogenesis of tympanosclerosis.
authors.affiliation: ENT Clinic, Elazığ Education and Research Hospital.
authors.forename: A
authors.initials: A
authors.lastname: Akyigit
authors.affiliation: Department of ENT,Firat University Faculty of Medicine,Elazığ,Turkey.
authors.forename: Ş
authors.initials: Ş
authors.lastname: Yalcin
authors.affiliation: Department of Medical Biology,Firat University Faculty of Medicine,Elazığ,Turkey.
authors.forename: E Ö
authors.initials: EÖ
authors.lastname: Etem
authors.affiliation: Department of ENT,Firat University Faculty of Medicine,Elazığ,Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Kaygusuz
authors.affiliation: Department of ENT,Firat University Faculty of Medicine,Elazığ,Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Karlidag
authors.affiliation: Department of ENT,Firat University Faculty of Medicine,Elazığ,Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Keles
authors.affiliation: Department of Medical Biology,Firat University Faculty of Medicine,Elazığ,Turkey.
authors.forename: Z B
authors.initials: ZB
authors.lastname: Celik
authors.affiliation: Department of Medical Biology,Firat University Faculty of Medicine,Elazığ,Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Tektemur
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D063371:Myringosclerosis / Q000097:blood / Q000201:enzymology*; D052247:Nitric Oxide Synthase Type II / Q000097:blood / Q000235:genetics*; D010033:Otitis Media / Q000097:blood / Q000201:enzymology*; D011110:Polymorphism, Genetic; D013482:Superoxide Dismutase / Q000097:blood / Q000235:genetics*; D000099156:Superoxide Dismutase 2
chemical_list: D000975:Antioxidants; D002374:Catalase; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2; C577219:CAT protein, human; C496319:NOS2 protein, human
keywords: Genetic; Tympanosclerosis; Nitric Oxide Synthase Type II; Superoxide Dismutase; Catalase; Polymorphism