title: PPP1, a plant-specific regulator of transcription controls Arabidopsis development and PIN expression.
abstract: Directional transport of auxin is essential for plant development, with PIN auxin transport proteins representing an integral part of the machinery that controls hormone distribution. However, unlike the rapidly emerging framework of molecular determinants regulating PIN protein abundance and subcellular localization, insights into mechanisms controlling PIN transcription are still limited. Here we describe PIN2 PROMOTER BINDING PROTEIN 1 (PPP1), an evolutionary conserved plant-specific DNA binding protein that acts on transcription of PIN genes. Consistent with PPP1 DNA-binding activity, PPP1 reporter proteins are nuclear localized and analysis of PPP1 null alleles and knockdown lines indicated a function as a positive regulator of PIN expression. Furthermore, we show that ppp1 pleiotropic mutant phenotypes are partially reverted by PIN overexpression, and results are presented that underline a role of PPP1-PIN promoter interaction in PIN expression control. Collectively, our findings identify an elementary, thus far unknown, plant-specific DNA-binding protein required for post-embryonic plant development, in general, and correct expression of PIN genes, in particular.
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.|Plant Developmental Biology, Wageningen University Research, 6708 PB Wageningen, The Netherlands.
authors.forename: René
authors.initials: R
authors.lastname: Benjamins
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Elke
authors.initials: E
authors.lastname: Barbez
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Ortbauer
authors.affiliation: Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, 1090 GE Amsterdam, The Netherlands.
authors.forename: Inez
authors.initials: I
authors.lastname: Terpstra
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Doris
authors.initials: D
authors.lastname: Lucyshyn
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Jeanette
authors.initials: J
authors.lastname: Moulinier-Anzola
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Muhammad Asaf
authors.initials: MA
authors.lastname: Khan
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Leitner
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Nenad
authors.initials: N
authors.lastname: Malenica
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Haroon
authors.initials: H
authors.lastname: Butt
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Barbara
authors.initials: B
authors.lastname: Korbei
authors.affiliation: Plant Developmental Biology, Wageningen University Research, 6708 PB Wageningen, The Netherlands.
authors.forename: Ben
authors.initials: B
authors.lastname: Scheres
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kleine-Vehn
authors.affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Luschnig
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D003198:Computer Simulation; D003593:Cytoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018519:Meristem / Q000502:physiology; D010802:Phylogeny; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D000072417:Protein Domains; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D016601:RNA-Binding Proteins; C117013:AT5G57090 protein, Arabidopsis; C000628068:PPP1 protein, Arabidopsis