title: MAGI-2 Is a Sensitive and Specific Marker of Prostatic Adenocarcinoma:  A Comparison With AMACR.
abstract: OBJECTIVE
  We compared the utility of membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 (MAGI-2) and α-methylacyl CoA (AMACR) by immunohistochemistry in diagnosing prostatic adenocarcinoma.
  
  
  METHODS
  Seventy-eight radical prostatectomies were used to construct three tissue microarrays with 512 cores, including benign prostatic tissue, benign prostatic hyperplasia, high-grade prostatic intraepithelial neoplasia (HGPIN), and adenocarcinoma. AMACR and MAGI-2 immunohistochemistry were evaluated by visual and image analysis.
  
  
  RESULTS
  MAGI-2 and AMACR were significantly higher in adenocarcinoma and HGPIN compared with benign tissue. At H-score cutoffs of 300 and 200, MAGI-2 was more accurate in distinguishing benign from malignant glands than AMACR. Areas under the curve by image and visual analysis were 0.846 and 0.818 for MAGI-2 and 0.937 and 0.924 for AMACR, respectively. The accuracy of MAGI-2 in distinguishing benign from malignant glands on the same core was higher (95% vs 88%).
  
  
  CONCLUSIONS
  MAGI-2 could represent a useful adjunct for diagnosis of prostatic adenocarcinoma, especially when AMACR is not discriminatory.
authors.affiliation: From the Department of Pathology, Microbiology, and Immunology.
authors.forename: Jeffery
authors.initials: J
authors.lastname: Goldstein
authors.affiliation: From the Department of Pathology, Microbiology, and Immunology.
authors.forename: Rajen
authors.initials: R
authors.lastname: Goyal
authors.affiliation: Department of Surgery.
authors.forename: Joseph T
authors.initials: JT
authors.lastname: Roland
authors.affiliation: From the Department of Pathology, Microbiology, and Immunology.
authors.forename: Lan L
authors.initials: LL
authors.lastname: Gellert
authors.affiliation: Department of Urologic Surgery, Vanderbilt University Medical Center, Nashville, TN.
authors.forename: Peter E
authors.initials: PE
authors.lastname: Clark
authors.affiliation: From the Department of Pathology, Microbiology, and Immunology Department of Urologic Surgery, Vanderbilt University Medical Center, Nashville, TN. omar.hameed@vanderbilt.edu.
authors.forename: Omar
authors.initials: O
authors.lastname: Hameed
authors.affiliation: From the Department of Pathology, Microbiology, and Immunology.
authors.forename: Giovanna A
authors.initials: GA
authors.lastname: Giannico
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000230:Adenocarcinoma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D003937:Diagnosis, Differential; D051528:Guanylate Kinases; D006801:Humans; D008297:Male; D011467:Prostate / Q000378:metabolism* / Q000473:pathology; D011468:Prostatectomy; D011470:Prostatic Hyperplasia / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D019048:Prostatic Intraepithelial Neoplasia / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D019998:Racemases and Epimerases / Q000378:metabolism*; D012680:Sensitivity and Specificity; D046888:Tissue Array Analysis
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D051528:Guanylate Kinases; C485777:MAGI2 protein, human; D019998:Racemases and Epimerases; C108352:alpha-methylacyl-CoA racemase
keywords: AMACR; MAGI-2; Prostate cancer