title: Landscape of RNAs in human lumbar disc degeneration.
abstract: Accumulating evidence indicates noncoding RNAs (ncRNAs) fine-tune gene expression with mysterious machinery. We conducted a combination of mRNA, miRNA, circRNA, LncRNA microarray analyses on 10 adults' lumbar discs. Moreover, we performed additional global exploration on RNA interacting machinery in terms of in silico computational pipeline. Here we show the landscape of RNAs in human lumbar discs. In general, the RNA-abundant landscape comprises 14,635 mRNAs (37.93%), 2,059 miRNAs (5.34%), 18,995 LncRNAs (49.23%) and 2,894 (7.5%) circRNAs. Chromosome 1 contributes for RNA transcription at most (10%). Bi-directional transcription contributes evenly for RNA biogenesis, in terms of 5' to 3' and 3' to 5'. Despite the majority of circRNAs are exonic, antisense (1.49%), intergenic (0.035%), intragenic (1.69%), and intronic (6.29%) circRNAs should not be ignored. A single miRNA could interact with a multitude of circRNAs. Notably, CDR1as or ciRS-7 harbors 66 consecutive binding sites for miR-7-5p (previous miR-7), evidencing our pipeline. The majority of binding sites are perfect-matched (78.95%). Collectively, global landscape of RNAs sheds novel insights on RNA interacting mechanisms in human intervertebral disc degeneration.
authors.affiliation: Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an, P. R. China.|Department of Orthopaedics, No. 5 Hospital of the PLA, Yinchuan, P. R. China.
authors.forename: Ping-Heng
authors.initials: PH
authors.lastname: Lan
authors.affiliation: Department of Orthopaedics, Xi'an Air Force Hospital, Xi'an, P.R. China.
authors.forename: Zhi-Heng
authors.initials: ZH
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an, P. R. China.
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Pei
authors.affiliation: The Second Department of Surgery, No. 518 Hospital of the PLA, Xi'an, P. R. China.|Department of Orthopaedic Surgery, Lanzhou General Hospital of Lanzhou Military Region, PLA, Lanzhou, P. R. China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi'an, P. R. China.|Shaanxi Key Laboratory of Stomatology, Biomaterials Unit, School of Stomatology, Fourth Military Medical University, Xi'an, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Company Eleven, Brigade of Undergraduates, Fourth Military Medical University, Xi'an, P. R. China.
authors.forename: Yong-Feng
authors.initials: YF
authors.lastname: Yang
authors.affiliation: Aerospace Medical School, Fourth Military Medical University, Xi'an, P. R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Aerospace Medical School, Fourth Military Medical University, Xi'an, P. R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Che
authors.affiliation: The First Hospital of Hanbin Area, Ankang, P. R. China.
authors.forename: Chi-Jiao
authors.initials: CJ
authors.lastname: Ma
authors.affiliation: Aerospace Medical School, Fourth Military Medical University, Xi'an, P. R. China.
authors.forename: Yan-Ke
authors.initials: YK
authors.lastname: Xie
authors.affiliation: Aerospace Medical School, Fourth Military Medical University, Xi'an, P. R. China.
authors.forename: Qing-Jie
authors.initials: QJ
authors.lastname: Hu
authors.affiliation: Department of Orthopedics, General Hospital of Beijing Military Command, Beijing, P. R. China.
authors.forename: Zhong-Yuan
authors.initials: ZY
authors.lastname: Wan
authors.affiliation: Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an, P. R. China.
authors.forename: Hai-Qiang
authors.initials: HQ
authors.lastname: Wang
mesh_terms: D000328:Adult; D001665:Binding Sites; D019295:Computational Biology; D015870:Gene Expression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D055959:Intervertebral Disc Degeneration / Q000235:genetics*; D008159:Lumbar Vertebrae / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D000070614:Nucleus Pulposus / Q000473:pathology; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D035683:MicroRNAs; D062085:RNA, Long Noncoding; D012333:RNA, Messenger
keywords: RNA transcription; circRNAs; gene regulation; miRNAs; noncoding RNAs