title: Using Fluorescent Reporters to Monitor Autophagy in the Female Germline Cells in Drosophila melanogaster.
abstract: Oogenesis is a fundamental biological process for the transmission of genetic information to the next generations. Drosophila has proven to be a valuable model for elucidating the molecular and cellular mechanisms involved in this developmental process. It has been shown that autophagy participates in the maturation of the egg chamber. Here we provide a protocol for monitoring and quantification of the autophagic process in the Drosophila germline cells using the fluorescent reporters mCherry-DmAtg8a and GFP-mCherry-DmAtg8a.
authors.affiliation: School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK.
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Jacomin
authors.affiliation: School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK. I.Nezis@warwick.ac.uk.
authors.forename: Ioannis P
authors.initials: IP
authors.lastname: Nezis
mesh_terms: D000818:Animals; D001343:Autophagy; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D005854:Germ Cells / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010053:Ovary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins
keywords: Autophagy; Drosophila; GAL4/UAS system; GFP-mCherry-DmAtg8a; Germline cells; mCherry-DmAtg8a