title: Clinical significance of TIPE expression in gastric carcinoma.
abstract: BACKGROUND
  TNFAIP8, also known as TIPE, is a suppressor of apoptosis. High expression of both TIPE mRNA and protein has been detected in various cancer cell lines and clinical specimens compared to healthy tissues. Many reports have shown that there is a strong correlation between TIPE overexpression and cancer progression and poor prognosis in human solid cancers.
  
  
  METHODS
  To illustrate the functional and clinical significance of TIPE in gastric cancer, we used reverse transcription polymerase chain reaction, quantitative real-time polymerase chain reaction, and immunohistochemistry to measure TIPE expression in clinical gastric specimens. Then, TIPE expression was knocked down by using shRNA and anti-DR5ScFv, to examine different expressions of TIPE in BGC823 cell lines, while cell proliferation and apoptosis were induced.
  
  
  RESULTS
  We found that there was a strong correlation between TIPE expression and TNM stage (P=0.044), tumor depth (P=0.016), lymph node metastasis (P=0.026), and distant metastasis (P=0.045). No significant correlation was found between TIPE expression with the patients' age (P=0.062) or sex (P=0.459). Anti-DR5ScFv induced TIPE depletion both in vitro and in vivo and resulted in apoptosis and suppression of proliferation.
  
  
  CONCLUSIONS
  Our results suggested that TIPE expression was associated with gastric cancer progression, and most importantly, suppressing TIPE expression might be an effective therapeutic strategy.
authors.affiliation: Organ Transplantation Institute, Anti-Cancer Research Center, Medical College, Xiamen University, Fujian, People's Republic of China.
authors.forename: Ruyi
authors.initials: R
authors.lastname: Hu
authors.affiliation: Organ Transplantation Institute, Anti-Cancer Research Center, Medical College, Xiamen University, Fujian, People's Republic of China; Division of Gastroenterology Surgery, Zhongshan Hospital, Gastroenterology Institute of Xiamen University, Gastroenterology Center of Xiamen, Fujian, People's Republic of China.
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Organ Transplantation Institute, Anti-Cancer Research Center, Medical College, Xiamen University, Fujian, People's Republic of China.
authors.forename: Shifu
authors.initials: S
authors.lastname: Hong
authors.affiliation: Organ Transplantation Institute, Anti-Cancer Research Center, Medical College, Xiamen University, Fujian, People's Republic of China.
authors.forename: Luxi
authors.initials: L
authors.lastname: Meng
authors.affiliation: Fujian Medical University, Fujian, People's Republic of China.
authors.forename: Xinya
authors.initials: X
authors.lastname: Hong
authors.affiliation: Organ Transplantation Institute, Anti-Cancer Research Center, Medical College, Xiamen University, Fujian, People's Republic of China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Organ Transplantation Institute, Anti-Cancer Research Center, Medical College, Xiamen University, Fujian, People's Republic of China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Organ Transplantation Institute, Anti-Cancer Research Center, Medical College, Xiamen University, Fujian, People's Republic of China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Zhuang
authors.affiliation: Organ Transplantation Institute, Anti-Cancer Research Center, Medical College, Xiamen University, Fujian, People's Republic of China; Department of General Surgery, The Tenth People's Hospital of Tongji University, Shanghai, People's Republic of China.
authors.forename: Zhongchen
authors.initials: Z
authors.lastname: Liu
keywords: TNFAIP8; anti-DR5ScFv; apoptosis; gastric cancer; metastasis; shRNA