title: Live Imaging of Intracellular Dynamics During Meiotic Maturation in Mouse Oocytes.
abstract: Fluorescence live imaging is a powerful approach to study intracellular dynamics during cellular events such as cell division. By applying automated confocal live imaging to mouse oocytes, in which meiotic maturation can be induced in vitro after the introduction of fluorescent proteins through microinjection, the meiotic dynamics of intracellular structures, such as chromosomes, can be monitored at high resolution. A combination of this method with approaches for the perturbation of specific proteins opens up opportunities for understanding the molecular and intracellular basis of mammalian meiosis.
authors.affiliation: Laboratory for Chromosome Segregation, RIKEN Center for Developmental Biology, 2-2-3 Minotojima-minamimachi, 650 0047, Kobe, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Laboratory for Chromosome Segregation, RIKEN Center for Developmental Biology, 2-2-3 Minotojima-minamimachi, 650 0047, Kobe, Japan.
authors.forename: Yogo
authors.initials: Y
authors.lastname: Sakakibara
authors.affiliation: Laboratory for Chromosome Segregation, RIKEN Center for Developmental Biology, 2-2-3 Minotojima-minamimachi, 650 0047, Kobe, Japan. tkitajima@cdb.riken.jp.
authors.forename: Tomoya S
authors.initials: TS
authors.lastname: Kitajima
mesh_terms: D000818:Animals; D015415:Biomarkers; D002469:Cell Separation / Q000379:methods; D005260:Female; D042541:Intracellular Space / Q000378:metabolism; D008540:Meiosis / Q000235:genetics; D051379:Mice; D008845:Microinjections / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D015415:Biomarkers; D012313:RNA
keywords: Chromosome; Live imaging; Meiosis; Oocyte