title: MicroXRF tomographic visualization of zinc and iron in the zebrafish embryo at the onset of the hatching period.
abstract: Transition metals such as zinc, copper, and iron play key roles in cellular proliferation, cell differentiation, growth, and development. Over the past decade, advances in synchrotron X-ray fluorescence instrumentation presented new opportunities for the three-dimensional mapping of trace metal distributions within intact specimens. Taking advantage of microXRF tomography, we visualized the 3D distribution of zinc and iron in a zebrafish embryo at the onset of the hatching period. The reconstructed volumetric data revealed distinct differences in the elemental distributions, with zinc predominantly localized to the yolk and yolk extension, and iron to various regions of the brain as well as the myotome extending along the dorsal side of the embryo. The data set complements an earlier tomographic study of an embryo at the pharyngula stage (24 hpf), thus offering new insights into the trace metal distribution at key stages of embryonic development.
authors.affiliation: School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332, USA. fahrni@chemistry.gatech.edu.
authors.forename: Daisy
authors.initials: D
authors.lastname: Bourassa
authors.affiliation: Advanced Photon Source, X-ray Science Division, Argonne National Laboratory, 9700 S. Cass Avenue, Argonne, IL 60439, USA.
authors.forename: Sophie-Charlotte
authors.initials: SC
authors.lastname: Gleber
authors.affiliation: Advanced Photon Source, X-ray Science Division, Argonne National Laboratory, 9700 S. Cass Avenue, Argonne, IL 60439, USA.
authors.forename: Stefan
authors.initials: S
authors.lastname: Vogt
authors.affiliation: School of Biological Sciences and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA 30332, USA.
authors.forename: Chong Hyun
authors.initials: CH
authors.lastname: Shin
authors.affiliation: School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332, USA. fahrni@chemistry.gatech.edu.
authors.forename: Christoph J
authors.initials: CJ
authors.lastname: Fahrni
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000737:chemistry* / Q000196:embryology / Q000378:metabolism; D005453:Fluorescence; D007501:Iron / Q000032:analysis* / Q000378:metabolism; D014056:Tomography, X-Ray; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism; D015032:Zinc / Q000032:analysis* / Q000378:metabolism
chemical_list: D007501:Iron; D015032:Zinc