title: The Metabotropic Glutamate Receptor Subtype 1 Mediates Experience-Dependent Maintenance of Mature Synaptic Connectivity in the Visual Thalamus.
abstract: Neural circuits formed during postnatal development have to be maintained stably thereafter, but their mechanisms remain largely unknown. Here we report that the metabotropic glutamate receptor subtype 1 (mGluR1) is essential for the maintenance of mature synaptic connectivity in the dorsal lateral geniculate nucleus (dLGN). In mGluR1 knockout (mGluR1-KO) mice, strengthening and elimination at retinogeniculate synapses occurred normally until around postnatal day 20 (P20). However, during the subsequent visual-experience-dependent maintenance phase, weak retinogeniculate synapses were newly recruited. These changes were similar to those of wild-type (WT) mice that underwent visual deprivation or inactivation of mGluR1 in the dLGN from P21. Importantly, visual deprivation was ineffective in mGluR1-KO mice, and the changes induced by visual deprivation in WT mice were rescued by pharmacological activation of mGluR1 in the dLGN. These results demonstrate that mGluR1 is crucial for the visual-experience-dependent maintenance of mature synaptic connectivity in the dLGN.
authors.affiliation: Department of Physiology, School of Medicine, Tokyo Women's Medical University, Tokyo 162-8666, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Narushima
authors.affiliation: Department of Anatomy, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan.
authors.forename: Motokazu
authors.initials: M
authors.lastname: Uchigashima
authors.affiliation: Department of Physiology, School of Medicine, Tokyo Women's Medical University, Tokyo 162-8666, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yagasaki
authors.affiliation: Labolatory of Animal Resources, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Harada
authors.affiliation: Department of Physiology, School of Medicine, Tokyo Women's Medical University, Tokyo 162-8666, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Nagumo
authors.affiliation: Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Naofumi
authors.initials: N
authors.lastname: Uesaka
authors.affiliation: Department of Neurophysiology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8551, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Hashimoto
authors.affiliation: Labolatory of Animal Resources, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Atsu
authors.initials: A
authors.lastname: Aiba
authors.affiliation: Department of Anatomy, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Department of Physiology, School of Medicine, Tokyo Women's Medical University, Tokyo 162-8666, Japan. Electronic address: mmiyata@twmu.ac.jp.
authors.forename: Mariko
authors.initials: M
authors.lastname: Miyata
authors.affiliation: Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan. Electronic address: mkano-tky@m.u-tokyo.ac.jp.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kano
mesh_terms: D000818:Animals; D002219:Carbamates / Q000494:pharmacology; D005829:Geniculate Bodies / Q000187:drug effects / Q000502:physiology*; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists / Q000235:genetics / Q000502:physiology*; D012118:Resorcinols / Q000494:pharmacology; D012160:Retina / Q000502:physiology; D012683:Sensory Deprivation / Q000502:physiology; D013569:Synapses / Q000502:physiology*; D013788:Thalamus / Q000502:physiology*; D014795:Visual Pathways / Q000502:physiology*; D014966:Xanthenes / Q000494:pharmacology
chemical_list: C573813:(9H-xanthene-9-carbonyl)carbamic acid butyl ester; D002219:Carbamates; D018094:Receptors, Metabotropic Glutamate; D012118:Resorcinols; D014966:Xanthenes; C104077:metabotropic glutamate receptor type 1; C079215:3,5-dihydroxyphenylglycine; D005998:Glycine