title: Reverse Fluorescence Enhancement and Colorimetric Bimodal Signal Readout Immunochromatography Test Strip for Ultrasensitive Large-Scale Screening and Postoperative Monitoring.
abstract: Ultrasensitive and quantitative fast screening of cancer biomarkers by immunochromatography test strip (ICTS) is still challenging in clinic. The gold nanoparticles (NPs) based ICTS with colorimetric readout enables a quick spectrum screening but suffers from nonquantitative performance; although ICTS with fluorescence readout (FICTS) allows quantitative detection, its sensitivity still deserves more efforts and attentions. In this work, by taking advantages of colorimetric ICTS and FICTS, we described a reverse fluorescence enhancement ICTS (rFICTS) with bimodal signal readout for ultrasensitive and quantitative fast screening of carcinoembryonic antigen (CEA). In the presence of target, gold NPs aggregation in T line induced colorimetric readout, allowing on-the-spot spectrum screening in 10 min by naked eye. Meanwhile, the reverse fluorescence enhancement signal enabled more accurately quantitative detection with better sensitivity (5.89 pg/mL for CEA), which is more than 2 orders of magnitude lower than that of the conventional FICTS. The accuracy and stability of the rFICTS were investigated with more than 100 clinical serum samples for large-scale screening. Furthermore, this rFICTS also realized postoperative monitoring by detecting CEA in a patient with colon cancer and comparing with CT imaging diagnosis. These results indicated this rFICTS is particularly suitable for point-of-care (POC) diagnostics in both resource-rich and resource-limited settings.
authors.affiliation: School of Materials Science and Engineering, School of Life Sciences, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Laboratory of Controllable Nanopharmaceuticals, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.
authors.forename: Weisheng
authors.initials: W
authors.lastname: Guo
authors.affiliation: School of Materials Science and Engineering, School of Life Sciences, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Materials Science and Engineering, School of Life Sciences, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of General Surgery, Tianjin Medical University General Hospital , Tianjin 300052, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Fu
authors.affiliation: Department of General Surgery, Tianjin Medical University General Hospital , Tianjin 300052, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.affiliation: School of Materials Science and Engineering, School of Life Sciences, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wu
authors.affiliation: School of Materials Science and Engineering, School of Life Sciences, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Hanjie
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, School of Life Sciences, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Gong
authors.affiliation: Laboratory of Controllable Nanopharmaceuticals, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.
authors.forename: Xing-Jie
authors.initials: XJ
authors.lastname: Liang
authors.affiliation: School of Materials Science and Engineering, School of Life Sciences, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chang
mesh_terms: D002272:Carcinoembryonic Antigen; D002846:Chromatography, Affinity; D003124:Colorimetry; D006046:Gold; D006801:Humans; D053768:Metal Nanoparticles
chemical_list: D002272:Carcinoembryonic Antigen; D006046:Gold
keywords: POCT; bimodal signal readout; carcinoembryonic antigen (CEA); postoperative monitoring; reverse fluorescence enhancement ICTS (rFICTS)