title: Effect of Quercetin on Hepatitis C Virus Life Cycle: From Viral to Host Targets.
abstract: Quercetin is a natural flavonoid, which has been shown to have anti hepatitis C virus (HCV) properties. However, the exact mechanisms whereby quercetin impacts the HCV life cycle are not fully understood. We assessed the effect of quercetin on different steps of the HCV life cycle in Huh-7.5 cells and primary human hepatocytes (PHH) infected with HCVcc. In both cell types, quercetin significantly decreased i) the viral genome replication; ii) the production of infectious HCV particles and iii) the specific infectivity of the newly produced viral particles (by 85% and 92%, Huh7.5 and PHH respectively). In addition, when applied directly on HCV particles, quercetin reduced their infectivity by 65%, suggesting that it affects the virion integrity. Interestingly, the HCV-induced up-regulation of diacylglycerol acyltransferase (DGAT) and the typical localization of the HCV core protein to the surface of lipid droplets, known to be mediated by DGAT, were both prevented by quercetin. In conclusion, quercetin appears to have direct and host-mediated antiviral effects against HCV.
authors.affiliation: UCM Digestive Diseases, Virgen Macarena-Virgen del Rocío University Hospitals and CIBERehd, Institute of Biomedicine, University of Sevilla, Sevilla, Spain.|Unit for the Clinical Management of Digestive Diseases, Hospital Universitario Valme de Sevilla, Sevilla, Spain.
authors.forename: Ángela
authors.initials: Á
authors.lastname: Rojas
authors.affiliation: Unit for the Clinical Management of Digestive Diseases, Hospital Universitario Valme de Sevilla, Sevilla, Spain.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Del Campo
authors.affiliation: Division of Clinical Pathology, University Hospital, Geneva, Switzerland.
authors.forename: Sophie
authors.initials: S
authors.lastname: Clement
authors.affiliation: University Paris Descartes, EA 4474 "Hepatitis C Virology", France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Lemasson
authors.affiliation: UCM Digestive Diseases, Virgen Macarena-Virgen del Rocío University Hospitals and CIBERehd, Institute of Biomedicine, University of Sevilla, Sevilla, Spain.|Unit for the Clinical Management of Digestive Diseases, Hospital Universitario Valme de Sevilla, Sevilla, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: García-Valdecasas
authors.affiliation: UCM Digestive Diseases, Virgen Macarena-Virgen del Rocío University Hospitals and CIBERehd, Institute of Biomedicine, University of Sevilla, Sevilla, Spain.|Unit for the Clinical Management of Digestive Diseases, Hospital Universitario Valme de Sevilla, Sevilla, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Gil-Gómez
authors.affiliation: Unit for the Clinical Management of Digestive Diseases, Hospital Universitario Valme de Sevilla, Sevilla, Spain.
authors.forename: Isidora
authors.initials: I
authors.lastname: Ranchal
authors.affiliation: Inserm U1052, Cancer Research Centre, University of Lyon, France DevWeCan Laboratories of Excellence Network (Labex), Lyon, France.
authors.forename: Birke
authors.initials: B
authors.lastname: Bartosch
authors.affiliation: Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Sevilla, Spain.
authors.forename: Juan D
authors.initials: JD
authors.lastname: Bautista
authors.affiliation: University Paris Descartes, EA 4474 "Hepatitis C Virology", France.
authors.forename: Arielle R
authors.initials: AR
authors.lastname: Rosenberg
authors.affiliation: Division of Clinical Pathology, University Hospital, Geneva, Switzerland.|Division of Gastroenterology and Hepatology, University Hospital, Geneva, Switzerland.
authors.forename: Francesco
authors.initials: F
authors.lastname: Negro
authors.affiliation: UCM Digestive Diseases, Virgen Macarena-Virgen del Rocío University Hospitals and CIBERehd, Institute of Biomedicine, University of Sevilla, Sevilla, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Romero-Gómez
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000821:virology; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D016174:Hepacivirus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000821:virology; D054884:Host-Pathogen Interactions / Q000187:drug effects; D006801:Humans; D066292:Lipid Droplets / Q000187:drug effects / Q000378:metabolism; D008113:Liver Neoplasms / Q000473:pathology / Q000821:virology; D011794:Quercetin / Q000494:pharmacology*; D014771:Virion / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects* / Q000235:genetics
chemical_list: D011794:Quercetin; D051048:Diacylglycerol O-Acyltransferase