title: Rapid Isolation and Determination of Flavones in Biological Samples Using Zinc Complexation Coupled with High-Performance Liquid Chromatography.
abstract: Chlorophyll-type contaminants are commonly encountered in the isolation and determination of flavones of plant aerial plant parts. Heme is also a difficult background substance in whole blood analysis. Both chlorophyll and heme are porphyrin type compounds. In this study, a rapid method for isolating flavones with 5-hydroxyl or ortho-hydroxyl groups from biological samples was developed based on the different solubilities of porphyrin-metal and flavone-metal complexes. It is important that other background substances, e.g., proteins and lipids, are also removed from flavones without an additional processing. The recoveries of scutellarin, baicalin, baicalein, wogonoside and wogonin, which are the primary constituents of Scutellaria baicalensis (skullcaps) were 99.65% ± 1.02%, 98.98% ± 0.73%, 99.65% ± 0.03%, 97.59% ± 0.09% and 95.19% ± 0.47%, respectively. As a sample pretreatment procedure, this method was coupled to high-performance liquid chromatography (HPLC) with good separation, sensitivity and linearity and was applied to determine the flavone content in different aerial parts of S. baicalensis and in dried blood spot samples.
authors.affiliation: Institute of Special Wild Animals and Plants, Chinese Academy of Agricultural Science, Changchun 130122, China. 18611297241@126.com.|School of Life Science, Beijing Institute of Technology, Beijing 100081, China. 18611297241@126.com.
authors.forename: Chenghe
authors.initials: C
authors.lastname: Sun
authors.affiliation: Institute of Special Wild Animals and Plants, Chinese Academy of Agricultural Science, Changchun 130122, China. whcxf332@163.com.|School of Life Science, Beijing Institute of Technology, Beijing 100081, China. whcxf332@163.com.
authors.forename: Hecheng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Special Wild Animals and Plants, Chinese Academy of Agricultural Science, Changchun 130122, China. yingpingw@126.com.
authors.forename: Yingping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Special Wild Animals and Plants, Chinese Academy of Agricultural Science, Changchun 130122, China. xiaoshengyuan@tsinghua.org.cn.|School of Life Science, Beijing Institute of Technology, Beijing 100081, China. xiaoshengyuan@tsinghua.org.cn.
authors.forename: Shengyuan
authors.initials: S
authors.lastname: Xiao
mesh_terms: D047310:Apigenin / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D059788:Dried Blood Spot Testing / Q000379:methods*; D044950:Flavanones / Q000302:isolation & purification; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification*; D005419:Flavonoids / Q000302:isolation & purification; D005960:Glucosides / Q000302:isolation & purification; D005965:Glucuronates / Q000302:isolation & purification; D006801:Humans; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry*; D019345:Zinc Acetate / Q000737:chemistry*
chemical_list: D044950:Flavanones; D047309:Flavones; D005419:Flavonoids; D005960:Glucosides; D005965:Glucuronates; D010936:Plant Extracts; C484876:scutellarin; C038044:baicalin; C006680:baicalein; D047310:Apigenin; C473995:wogonoside; D019345:Zinc Acetate; C085514:wogonin
keywords: Scutellaria baicalensis; chlorophyll; dried blood spot; heme; high-performance liquid chromatography; metal complexation