title: Interaction of polyethyleneimine-anchored copper(II) complexes with tRNA studied by spectroscopy methods and biological activities.
abstract: Ultraviolet-visible, emission and circular dichroism spectroscopic methods were used in transfer RNA (tRNA) interaction studies performed for polyethyleneimine-copper(II) complexes [Cu(phen)(l-Tyr)BPEI]ClO4(where phen =1,10-phenanthroline, l-Tyr = l-tyrosine and BPEI = branched polyethyleneimine) with various degrees of coordination (x = 0.059, 0.149, 0.182) in the polymer chain. The results indicated that polyethyleneimine-copper(II) complexes bind with tRNA mostly through surface binding, although other binding modes, such as hydrogen bonding and van der Waals interactions, might also be present. Dye-exclusion, sulforhodamine B and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays of a polyethyleneimine-copper(II) complex with a higher degree of coordination against different cancer cell lines proved that the complex exhibited cytotoxic specificity and a significant cancer cell inhibition rate. Antimicrobial screening showed activity against some human pathogens.
authors.affiliation: School of Chemistry, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India.
authors.forename: Jagadeesan
authors.initials: J
authors.lastname: Lakshmipraba
authors.affiliation: School of Chemistry, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India.
authors.forename: Sankaralingam
authors.initials: S
authors.lastname: Arunachalam
authors.affiliation: Department of Biotechnology, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India.
authors.forename: Devadas A
authors.initials: DA
authors.lastname: Gandi
authors.affiliation: Department of Biotechnology, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India.
authors.forename: Thyagarajan
authors.initials: T
authors.lastname: Thirunalasundari
authors.affiliation: Department of Marine Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India.
authors.forename: Sivanandham
authors.initials: S
authors.lastname: Vignesh
authors.affiliation: Department of Marine Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India.
authors.forename: Rathinam A
authors.initials: RA
authors.lastname: James
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003300:Copper / Q000737:chemistry* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005658:Fungi / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011094:Polyethyleneimine / Q000737:chemistry* / Q000494:pharmacology; D012343:RNA, Transfer / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D003300:Copper; D011094:Polyethyleneimine; D012343:RNA, Transfer
keywords: branched polyethyleneimine; copper(II) complex; cytotoxic studies; tRNA interaction