title: Anti-Tumor Effects of the Polysaccharide Isolated from Tarphochlamys Affinis in H22 Tumor-Bearing Mice.
abstract: BACKGROUND
  The previous studies have demonstrated that the polysaccharide isolated from Tarphochlamys affinis (PTA) exhibits anti-tumor effect on S180 tumor-bearing mice and protective effects against hepatic injury.
  
  
  METHODS
  In this study, we investigated the anti-tumor activity and possible underlying mechanism of PTA on liver cancer using a murine H22 hepatocarcinoma model.
  
  
  RESULTS
  PTA was capable of repressing transplanted H22 solid hepatic tumor cell growth in vivo. The relative weight of immune organs (spleen and thymus) and lymphocyte proliferation induced by ConA or LPS were improved after PTA treatment. Furthermore, treatment with PTA promoted immune-stimulating serum cytokine secretion in H22 tumor-bearing mice. Additionally, the percentage of CD4+ T lymphocytes, CD8+ T lymphocytes and NK cells was increased in tumor-bearing mice following PTA administration. In tumor tissue, PTA significantly up-regulated the expression of Bax and p53 proteins and down-regulated the expression of Bcl-2 protein. In addition, at the therapeutic dose, PTA displayed very few toxic effects to major organs, such as the liver and kidney, in tumor-bearing mice.
  
  
  CONCLUSIONS
  In H22 tumor-bearing mice, PTA exhibited prominent anti-tumor activity in vivo. The possible mechanism of action might be related to enhanced host immune system function and induction of H22 tumor cell apoptosis.
authors.affiliation: Department of Laboratory Medicine, Guangxi Medical College, Nanning, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Tang
authors.forename: Jianchun
authors.initials: J
authors.lastname: Huang
authors.forename: Hao
authors.initials: H
authors.lastname: Xiong
authors.forename: Keyuan
authors.initials: K
authors.lastname: Zhang
authors.forename: Chunxia
authors.initials: C
authors.lastname: Chen
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wei
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Xu
authors.forename: Qiuqiao
authors.initials: Q
authors.lastname: Xie
authors.forename: Renbin
authors.initials: R
authors.lastname: Huang
mesh_terms: D027901:Acanthaceae / Q000737:chemistry*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D007279:Injections, Subcutaneous; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051379:Mice; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D061251:Primary Cell Culture; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000819:agonists / Q000235:genetics / Q000276:immunology; D051028:bcl-2-Associated X Protein / Q000819:agonists / Q000235:genetics / Q000276:immunology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C494827:Bax protein, mouse; D008070:Lipopolysaccharides; D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D003208:Concanavalin A; C052617:Bcl2 protein, mouse