title: Grape Powder Improves Age-Related Decline in Mitochondrial and Kidney Functions in Fischer 344 Rats.
abstract: We examined the effects and mechanism of grape powder- (GP-) mediated improvement, if any, on aging kidney function. Adult (3-month) and aged (21-month) Fischer 344 rats were treated without (controls) and with GP (1.5% in drinking water) and kidney parameters were measured. Control aged rats showed higher levels of proteinuria and urinary kidney injury molecule-1 (KIM-1), which decreased with GP treatment in these rats. Renal protein carbonyls (protein oxidation) and gp (91phox) -NADPH oxidase levels were high in control aged rats, suggesting oxidative stress burden in these rats. GP treatment in aged rats restored these parameters to the levels of adult rats. Moreover, glomerular filtration rate and sodium excretion were low in control aged rats suggesting compromised kidney function, which improved with GP treatment in aged rats. Interestingly, low renal mitochondrial respiration and ATP levels in control aged rats were associated with reduced levels of mitochondrial biogenesis marker MtTFA. Also, Nrf2 proteins levels were reduced in control aged rats. GP treatment increased levels of MtTFA and Nrf2 in aged rats. These results suggest that GP by potentially regulating Nrf2 improves aging mitochondrial and kidney functions.
authors.affiliation: Heart and Kidney Institute, Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston, TX 77204, USA.
authors.forename: Indira
authors.identifier: 0000-0003-0669-9724
authors.initials: I
authors.lastname: Pokkunuri
authors.affiliation: University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors.forename: Quaisar
authors.initials: Q
authors.lastname: Ali
authors.affiliation: Heart and Kidney Institute, Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston, TX 77204, USA.
authors.forename: Mohammad
authors.identifier: 0000-0003-0430-053X
authors.initials: M
authors.lastname: Asghar
mesh_terms: D000375:Aging / Q000187:drug effects / Q000473:pathology*; D000818:Animals; D007668:Kidney / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000473:pathology*; D051267:NF-E2-Related Factor 2; D001678:Organelle Biogenesis; D010936:Plant Extracts / Q000494:pharmacology*; D011208:Powders; D011916:Rats, Inbred F344; D027843:Vitis / Q000737:chemistry*
chemical_list: D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D010936:Plant Extracts; D011208:Powders