title: Efficient Enzyme-Free Biomimetic Sensors for Natural Phenol Detection.
abstract: The development of sensors and biosensors based on copper enzymes and/or copper oxides for phenol sensing is disclosed in this work. The electrochemical properties were studied by cyclic and differential pulse voltammetry using standard solutions of potassium ferrocyanide, phosphate/acetate buffers and representative natural phenols in a wide pH range (3.0 to 9.0). Among the natural phenols herein investigated, the highest sensitivity was observed for rutin, a powerful antioxidant widespread in functional foods and ubiquitous in the plant kingdom. The calibration curve for rutin performed at optimum pH (7.0) was linear in a broad concentration range, 1 to 120 µM (r = 0.99), showing detection limits of 0.4 µM. The optimized biomimetic sensor was also applied in total phenol determination in natural samples, exhibiting higher stability and sensitivity as well as distinct selectivity for antioxidant compounds.
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, GO 74690-970, Brazil. luane.fg@hotmail.com.
authors.forename: Luane
authors.initials: L
authors.lastname: Ferreira Garcia
authors.affiliation: Instituto de Química, Universidade Federal de Goiás, Goiânia, GO 74690-970, Brazil. desouzaar@gmail.com.
authors.forename: Aparecido
authors.initials: A
authors.lastname: Ribeiro Souza
authors.affiliation: Instituto de Química, Universidade Federal de Goiás, Goiânia, GO 74690-970, Brazil. manger84@gmail.com.
authors.forename: Germán
authors.initials: G
authors.lastname: Sanz Lobón
authors.affiliation: Departamento de Farmácia, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, MG 74690-970, Brazil. wallanst@yahoo.com.br.
authors.forename: Wallans Torres Pio
authors.initials: WT
authors.lastname: Dos Santos
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, GO 74690-970, Brazil. morgana.alecrim@hotmail.com.
authors.forename: Morgana Fernandes
authors.initials: MF
authors.lastname: Alecrim
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, GO 74690-970, Brazil. mariangelafs@gmail.com.
authors.forename: Mariângela
authors.initials: M
authors.lastname: Fontes Santiago
authors.affiliation: Departamento de Química Orgánica, Universidad de Cordoba, Cordoba 14014, Spain. q62alsor@uco.es.
authors.forename: Rafael Luque Álvarez
authors.initials: RL
authors.lastname: de Sotomayor
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, GO 74690-970, Brazil. ericsgil@gmail.com.
authors.forename: Eric
authors.initials: E
authors.lastname: de Souza Gil
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001688:Biological Products / Q000032:analysis / Q000737:chemistry*; D032701:Biomimetics / Q000379:methods; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006108:Graphite / Q000737:chemistry; D010636:Phenols / Q000032:analysis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001688:Biological Products; D010636:Phenols; D006108:Graphite
keywords: enzyme-free biosensors; metal oxides; modified electrodes; rutin