title: Generation of a novel transgenic rat model for tracing extracellular vesicles in body fluids.
abstract: Extracellular vesicles (EVs) play an important role in the transfer of biomolecules between cells. To elucidate the intercellular transfer fate of EVs in vivo, we generated a new transgenic (Tg) rat model using green fluorescent protein (GFP)-tagged human CD63. CD63 protein is highly enriched on EV membranes via trafficking into late endosomes and is often used as an EV marker. The new Tg rat line in which human CD63-GFP is under control of the CAG promoter exhibited high expression of GFP in various body tissues. Exogenous human CD63-GFP was detected on EVs isolated from three body fluids of the Tg rats: blood serum, breast milk and amniotic fluid. In vitro culture allowed transfer of serum-derived CD63-GFP EVs into recipient rat embryonic fibroblasts, where the EVs localized in endocytic organelles. These results suggested that this Tg rat model should provide significant information for understanding the intercellular transfer and/or mother-child transfer of EVs in vivo.
authors.affiliation: Division of Laboratory Animals Resources, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), Tokyo, Japan.|Department of Mental Disorder Research, National Institute of Neuroscience, NCNP, Tokyo, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Yoshimura
authors.affiliation: Division of Molecular and Cellular medicine, National Cancer Center Research Institute (NCC), Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Kawamata
authors.affiliation: Division of Molecular and Cellular medicine, National Cancer Center Research Institute (NCC), Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yoshioka
authors.affiliation: Division of Molecular and Cellular medicine, National Cancer Center Research Institute (NCC), Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Katsuda
authors.affiliation: Department of Degenerative Neurological Diseases, National Institute of Neuroscience, NCNP, Tokyo, Japan.
authors.forename: Hisae
authors.initials: H
authors.lastname: Kikuchi
authors.affiliation: Department of Degenerative Neurological Diseases, National Institute of Neuroscience, NCNP, Tokyo, Japan.|Department of Neurotherapeutics, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nagai
authors.affiliation: Department of Mental Disorder Research, National Institute of Neuroscience, NCNP, Tokyo, Japan.|Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University, Sanda, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Adachi
authors.affiliation: Department of Mental Disorder Research, National Institute of Neuroscience, NCNP, Tokyo, Japan.|Department of Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Numakawa
authors.affiliation: Department of Mental Disorder Research, National Institute of Neuroscience, NCNP, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kunugi
authors.affiliation: Division of Molecular and Cellular medicine, National Cancer Center Research Institute (NCC), Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ochiya
authors.affiliation: Division of Laboratory Animals Resources, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), Tokyo, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Tamai
mesh_terms: D000653:Amniotic Fluid / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D001826:Body Fluids / Q000378:metabolism*; D002478:Cells, Cultured; D011992:Endosomes / Q000378:metabolism; D000067128:Extracellular Vesicles / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008431:Maternal-Fetal Exchange; D008892:Milk / Q000378:metabolism; D023421:Models, Animal; D011247:Pregnancy; D051381:Rats; D055647:Rats, Transgenic; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000097:blood / Q000235:genetics / Q000378:metabolism; D060149:Tetraspanin 30 / Q000097:blood / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: C554586:CD63 protein, human; D011993:Recombinant Fusion Proteins; D060149:Tetraspanin 30