title: Immunohistochemistry of Paraffin Sections from Mouse Ovaries.
abstract: Immunohistochemistry (IHC) is an efficient technique to detect cellular localizations of the proteins in paraffin-embedded tissues. It allows specific proteins to be visualized by the interaction of antibodies with an enzyme-substrate-chromogen system. Here, we describe indirect immunohistochemistry method for paraffin-embedded mouse ovaries fixed with Bouin's Fixative.
authors.affiliation: Department of Histology and Embryology, Medical Faculty, Akdeniz University, Antalya, 07070, Turkey. gokhan_akkoyunlu@hotmail.com.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Akkoyunlu
authors.affiliation: Department of Histology and Embryology, Medical Faculty, Akdeniz University, Antalya, 07070, Turkey.
authors.forename: Filiz
authors.initials: F
authors.lastname: Tepekoy
mesh_terms: D000818:Animals; D005260:Female; D016591:Histocytological Preparation Techniques; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D010053:Ovary / Q000378:metabolism*; D016612:Paraffin Embedding
keywords: Biotinylated antibodies; Bouin’s Fixative; Immunohistochemistry; Mouse ovary; Paraffin sections