title: Preparation and characterization of hemoglobin-silver composites as biocompatible antiseptics.
abstract: Microbial contamination has been a major challenge in a wide variety of fields such as biomedical and biomaterial applications. The development of biomaterials that possess excellent antibacterial ability and biocompatibility is of great importance to enhance the service life of biomaterials. In this study, the main protein component of red blood cells, hemoglobin (Hb), was employed to prepare Ag-Hb nanocomposites as novel biocompatible antiseptics. The formation of Ag-Hb nanocomposites on the titanium substrate are confirmed by field-emission scanning electron microscopy, Fourier transformed infrared spectroscopic, contact angles, and inductively coupled plasma atomic emission spectrometry analysis. The Ag-Hb titanium shows potent antibacterial ability against planktonic bacteria in the suspension and ability to prevent bacterial adhesion. Moreover, the Ag-Hb titanium shows excellent biocompatibility, which supports healthy osteoblast cellular activity and osteoblast differentiation. The results indicate that the Ag-Hb nanocomposites can be potentially useful for the fabrication of biomaterials for long-term applications.
authors.affiliation: College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China School of Chemistry and Chemical Engineering, Guangxi University, Nanning, China Department of Chemistry, North Carolina State University, Raleigh, NC, USA.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning, China.
authors.forename: Zhangfa
authors.initials: Z
authors.lastname: Tong
authors.affiliation: College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China.
authors.forename: Zhiruo
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Key Laboratory of Environment Change and Resources Use in Beibu Gulf (Guangxi Teachers Education University), Ministry of Education, China suwmail@163.com zhftong@sina.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Su
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000138:chemical synthesis; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage* / Q000138:chemical synthesis; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000138:chemical synthesis; D004203:Disinfection / Q000379:methods; D006454:Hemoglobins / Q000008:administration & dosage / Q000737:chemistry*; D008422:Materials Testing; D053761:Nanocomposites / Q000008:administration & dosage* / Q000737:chemistry; D012834:Silver / Q000008:administration & dosage* / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D001672:Biocompatible Materials; D006454:Hemoglobins; D012834:Silver
keywords: Hb; antibacterial; biocompatibility; osteoblast; silver