title: Short Communication Development of microsatellite markers and genetic diversity analysis for Pelodiscus sinensis.
abstract: Pelodiscus sinensis is a common freshwater soft-shell turtle found in China, and is an important aquaculture species. In this study, 20 polymorphic microsatellite primers were developed from the transcriptome. The genetic diversity of three populations of P. sinensis was evaluated, using 72 individuals. The number of alleles per locus ranged from 3 to 26. The observed and expected heterozygosities varied from 0.208 to 0.958, and from 0.302 to 0.963, respectively. The polymorphic information content varied from 0.283 to 0.953. No significant linkage disequilibrium was detected. These markers will be useful for future population genetic studies and molecular breeding of P. sinensis.
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.|College of Life Science and Technology, Shanghai Ocean University, Shanghai, China.
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.|College of Life Science and Technology, Shanghai Ocean University, Shanghai, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Hong
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China zhuxinping_1964@163.com.|College of Life Science and Technology, Shanghai Ocean University, Shanghai, China zhuxinping_1964@163.com.
authors.forename: X P
authors.initials: XP
authors.lastname: Zhu
mesh_terms: D000818:Animals; D017756:Aquaculture; D001947:Breeding; D006579:Heterozygote; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D010802:Phylogeny; D011110:Polymorphism, Genetic; D059467:Transcriptome; D014426:Turtles / Q000235:genetics*