title: Irisolidone attenuates ethanol-induced gastric injury in mice by inhibiting the infiltration of neutrophils.
abstract: This study was designed to determine whether irisolidone and its glycoside kakkalide, which are the major constituents of the flower of Pueraria lobata (Kudzu) can attenuate ethanol-induced gastritic injury in mice.
  
  
  
  Irisolidone and kakkalide inhibited IL-8 secretion and NF-κB activation in lipopolysaccharide-stimulated KATO III cells. Therefore, we investigated their protective effects against ethanol-induced gastric injury in mice. Pretreatment with kakkalide or irisolidone decreased the area of hemorrhagic ulcerative lesions caused by ethanol and suppressed stomach myeloperoxidase activity, CXCL4 secretion, and NF-κB activation. The ameliorating effect of irisolidone was more potent than that of kakkalide.
  
  
  
  Irisolidone may attenuate ethanol-induced gastritis by inhibiting the infiltration of immune cells, particularly neutrophils, through the regulation of CXCL-4 or IL-8 secretion.
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Pharmacy, Kyung Hee University, Dongdaemun-gu, Seoul, Korea.
authors.forename: Geum-Dan
authors.initials: GD
authors.lastname: Kang
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Pharmacy, Kyung Hee University, Dongdaemun-gu, Seoul, Korea.
authors.forename: Sang-Yoon
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Kyung Hee University, Dongdaemun-gu, Seoul, Korea.
authors.forename: Se-Eun
authors.initials: SE
authors.lastname: Jang
authors.affiliation: Department of Food and Nutrition, Kyung Hee University, Dongdaemun-gu, Seoul, Korea.
authors.forename: Myung Joo
authors.initials: MJ
authors.lastname: Han
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Pharmacy, Kyung Hee University, Dongdaemun-gu, Seoul, Korea.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D000431:Ethanol / Q000009:adverse effects*; D005419:Flavonoids / Q000494:pharmacology*; D005756:Gastritis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D006027:Glycosides / Q000494:pharmacology; D016209:Interleukin-8 / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D010978:Platelet Factor 4 / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D005419:Flavonoids; D006027:Glycosides; D016209:Interleukin-8; D007529:Isoflavones; D008070:Lipopolysaccharides; D016328:NF-kappa B; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; C423902:irisolidone; D010978:Platelet Factor 4; D000431:Ethanol; C108698:kakkalide; D009195:Peroxidase
keywords: Ethanol; Gastritis; Irisolidone; Kudzu; Neutrophil