title: TGEV infection up-regulates FcRn expression via activation of NF-κB signaling.
abstract: It has been well characterized that the neonatal Fc receptor (FcRn) transports maternal IgG to a fetus or newborn and protects IgG from degradation. We previously reported that FcRn is expressed in a model of normal porcine intestinal epithelial cells (IPEC-J2). Transmissible gastroenteritis is an acute enteric disease of swine that is caused by transmissible gastroenteritis virus (TGEV). How porcine FcRn (pFcRn) expression is regulated by pathogenic infection remains unknown. Our research shows that IPEC-J2 cells infected with TGEV had up-regulated pFcRn expression. In addition, the NF-κB signaling pathway was activated in IPEC-J2 cells by TGEV infection. Furthermore, treatment of TGEV-infected IPEC-J2 cells with the NF-κB-specific inhibitor BAY 11-7082 resulted in down-regulation of pFcRn expression. Transient transfection of pFcRn promoter luciferase report plasmids with overexpression of NF-κB p65 transcription factor enhanced the activation of the luciferase report plasmids. We identified four NF-κB transcription factor binding sites in the promoter region of this gene using luciferase reporter system, chromatin immunoprecipitation, electromobility shift assay, and supershift analysis. Together, the data provide the first evidence that TGEV infection up-regulates pFcRn expression via activation of NF-κB signaling.
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jinyue
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Shaoju
authors.initials: S
authors.lastname: Qian
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.|State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Dingren
authors.initials: D
authors.lastname: Bi
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.|State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Qigai
authors.initials: Q
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.|State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.|State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Luo
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.|State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Shaowen
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.|State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Xianrong
authors.initials: X
authors.lastname: Meng
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.|State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Zili
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D005761:Gastroenteritis, Transmissible, of Swine / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005786:Gene Expression Regulation; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011961:Receptors, Fc / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction; D013450:Sulfones / Q000494:pharmacology; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000472:pathogenicity
chemical_list: C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; D015395:Histocompatibility Antigens Class I; D016328:NF-kappa B; D009570:Nitriles; D011961:Receptors, Fc; D013450:Sulfones; C100688:Fc receptor, neonatal