title: Gel-free/label-free proteomic analysis of wheat shoot in stress tolerant varieties under iron nanoparticles exposure.
abstract: Iron nanoparticles (Fe NPs) have stimulatory effects on the germination ratio and plant growth of wheat. To elucidate the effects of Fe NPs on shoot of drought tolerant Pakistan-13 and salt tolerant NARC-11, a gel-free/label-free proteomic technique was used. The weights/lengths of seedling, shoot, and root of wheat varieties were increased on 5ppm Fe NPs exposure. The number of proteins related to photosynthesis and protein metabolism was decreased and increased in drought tolerant variety and salt tolerant variety, respectively, treated with Fe NPs compared to untreated plants. Differentially changed proteins in drought tolerant variety and salt tolerant variety were mainly related to photosynthesis. Out of photosynthesis related proteins, light reaction was enhanced in salt tolerant variety compared to drought tolerant variety on Fe NPs exposure. The abundance of ribulose bisphosphate carboxylase/oxygenase small chain in drought tolerant variety was higher than that in salt tolerant variety; however, in salt tolerant variety, it was increased 3 fold by Fe NPs exposure compared to untreated plant. These results suggest that Fe NPs improve the growth of wheat seedling, which might be associated with the increase of protein abundance in photosynthesis in salt tolerant variety.
authors.affiliation: Department of Botany, PMAS Arid Agriculture University, Rawalpindi 46300, Pakistan; National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
authors.forename: Farhat
authors.initials: F
authors.lastname: Yasmeen
authors.affiliation: Department of Botany, PMAS Arid Agriculture University, Rawalpindi 46300, Pakistan. Electronic address: drnaveedraja@uaar.edu.pk.
authors.forename: Naveed Iqbal
authors.initials: NI
authors.lastname: Raja
authors.affiliation: Department of Agronomy, PMAS Arid Agriculture University, Rawalpindi 46300, Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Razzaq
authors.affiliation: National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan. Electronic address: skomatsu@affrc.go.jp.
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
mesh_terms: D055864:Droughts; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D018525:Germination / Q000187:drug effects; D007501:Iron / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000633:toxicity*; D058977:Molecular Sequence Annotation; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics* / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D014908:Triticum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012965:Sodium Chloride; D007501:Iron; D012273:Ribulose-Bisphosphate Carboxylase
keywords: Drought tolerance; Iron nanoparticles; Proteomics; Salt tolerance; Shoot; Wheat