title: The Mutual Interactions between Mesenchymal Stem Cells and Myoblasts in an Autologous Co-Culture Model.
abstract: Both myoblasts and mesenchymal stem cells (MSC) take part in the muscle tissue regeneration and have been used as experimental cellular therapy in muscular disorders treatment. It is possible that co-transplantation approach could improve the efficacy of this treatment. However, the relations between those two cell types are not clearly defined. The aim of this study was to determine the reciprocal interactions between myoblasts and MSC in vitro in terms of the features important for the muscle regeneration process. Primary caprine muscle-derived cells (MDC) and bone marrow-derived MSC were analysed in autologous settings. We found that MSC contribute to myotubes formation by fusion with MDC when co-cultured directly, but do not acquire myogenic phenotype if exposed to MDC-derived soluble factors only. Experiments with exposure to hydrogen peroxide showed that MSC are significantly more resistant to oxidative stress than MDC, but a direct co-culture with MSC does not diminish the cytotoxic effect of H2O2 on MDC. Cell migration assay demonstrated that MSC possess significantly greater migration ability than MDC which is further enhanced by MDC-derived soluble factors, whereas the opposite effect was not found. MSC-derived soluble factors significantly enhanced the proliferation of MDC, whereas MDC inhibited the division rate of MSC. To conclude, presented results suggest that myogenic precursors and MSC support each other during muscle regeneration and therefore myoblasts-MSC co-transplantation could be an attractive approach in the treatment of muscular disorders.
authors.affiliation: Department of Immunology, Transplant Medicine and Internal Diseases, Transplantation Institute, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kulesza
authors.affiliation: Department of Immunology, Transplant Medicine and Internal Diseases, Transplantation Institute, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Burdzinska
authors.affiliation: Department of Immunology, Transplant Medicine and Internal Diseases, Transplantation Institute, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Szczepanska
authors.affiliation: Department of Immunology, Transplant Medicine and Internal Diseases, Transplantation Institute, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Weronika
authors.initials: W
authors.lastname: Zarychta-Wisniewska
authors.affiliation: Mossakowski Medical Research Centre, Electron Microscopy Platform, Polish Academy of Science, 5 Pawińskiego St., Warsaw, Poland.|Warsaw University of Life Sciences - SGGW, Department of Physiological Sciences, Faculty of Veterinary Medicine, 159 Nowoursynowska St., Warsaw, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Pajak
authors.affiliation: Department of Immunology, Center of Biostructure Research, Medical University of Warsaw, Warsaw, Poland.|Postgraduate School of Molecular Medicine, Medical University of Warsaw, 61 Żwirki i Wigury St., Warsaw, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Bojarczuk
authors.affiliation: Department of Urology, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Dybowski
authors.affiliation: Department of Immunology, Transplant Medicine and Internal Diseases, Transplantation Institute, Medical University of Warsaw, Warsaw, Poland.|Department of Bioinformatics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 5a Pawińskiego St., Warsaw, Poland.
authors.forename: Leszek
authors.initials: L
authors.lastname: Paczek
mesh_terms: D000818:Animals; D015415:Biomarkers; D002450:Cell Communication; D002454:Cell Differentiation; D002459:Cell Fusion; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D006041:Goats; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D024510:Muscle Development; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D006861:Hydrogen Peroxide