title: Short-term culture of monocytes as an in vitro evaluation system for bionanomaterials designated for medical use.
abstract: We studied the feasibility of using a short-term culture of monocytes, isolated from peripheral donor blood, to assess the biological activity of different types of bionanomaterials (BNM): biodegradable polimeric particles, fiber and film substrates of micro- and nano-dimensions, fullerenes (F) and nanodiamonds (ND), which are either currently in use and/or potentially applicable in medicine. Additionally, the effect of creating a protein corona on ND and F particles was investigated. The cellular reduction of (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a well-established tool for assessing the viability/metabolic activity of cells. The scanning electron microscopy assay can detect fine changes in cell morphology. In the present study BNM have been shown to affect; in a size, chemical composition and morphological characteristics-dependent manner, the ability of monocytes to reduce MTT as well as their morphology. Moreover, the specific effects of ND and F on MTT reduction and cell morphology were exhibited in a dose-dependent manner and sensitive to the formation of surface protein corona. Our results suggest that short-term culture of monocytes is a sensitive model system for assessing the biological effects of BMPs in vitro.
authors.affiliation: Siberian Federal University, Svobodnuy Av. 79, Krasnoyarsk, 660041, Russia.
authors.forename: Ekaterina Igorevna
authors.initials: EI
authors.lastname: Shishatskaya
authors.affiliation: Laboratory of Anatomy, Histology, Embryology, School of Medicine, University of Crete, Heraklion, 71003, Greece. Electronic address: nikitovic@uoc.gr.
authors.forename: Dragana
authors.initials: D
authors.lastname: Nikitovic
authors.affiliation: Institute of Physics, Russian Academy of Science, Siberian Division, Akademgorodok, 50, Build. 38, Krasnoyarsk, 660036, Russia.
authors.forename: Alexander Shabanov
authors.initials: AS
authors.lastname: Vasilievich
authors.affiliation: Laboratory of Anatomy, Histology, Embryology, School of Medicine, University of Crete, Heraklion, 71003, Greece.
authors.forename: George N
authors.initials: GN
authors.lastname: Tzanakakis
authors.affiliation: Laboratory of Toxicology, Medical School, University of Crete, Heraklion, 71003, Greece. Electronic address: aris@med.uoc.gr.
authors.forename: Aristidis M
authors.initials: AM
authors.lastname: Tsatsakis
authors.affiliation: Siberian Federal University, Svobodnuy Av. 79, Krasnoyarsk, 660041, Russia.
authors.forename: Natalia Gennadievna
authors.initials: NG
authors.lastname: Menzianova
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D018130:Diamond / Q000737:chemistry*; D037741:Fullerenes / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D053768:Metal Nanoparticles / Q000737:chemistry / Q000627:therapeutic use* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009000:Monocytes / Q000648:ultrastructure*
chemical_list: D037741:Fullerenes; D018130:Diamond
keywords: Bio-nanomaterials; Biocompatible biopolymers; Fullerenes; Monocytes; Nanodiamonds; Nanomaterials; Polyhydroxyalkanoates