title: The proteome of normal human retrobulbar optic nerve and sclera.
abstract: The optic nerve is a white matter tract that conveys visual information to the brain. The sclera comprises the white, protective outer layer of the eye. A characterization of the proteome of normal human retrobulbar optic nerve and sclera may facilitate studies of the eye. We conducted a proteomic analysis of optic nerve and sclera from five adults. Proteins were fractionated using SDS-PAGE. After in-gel digestion, peptides were analyzed using LC-MS/MS on an Orbitrap Elite mass spectrometer. We identified 2711 non-redundant proteins in retrobulbar optic nerve and 1945 non-redundant proteins in sclera. Optic nerve proteins included proteins expressed by oligodendrocytes (laminin, proteolipid protein, fibronectin), myelin proteins (myelin basic protein, myelin-associated glycoprotein), and paranodal structural proteins (ankyrin β, spectrin). Sclera included 18 collagen protein chains, small leucine-rich proteoglycans (decorin, biglycan, lumican, keratocan, prolargin, fibromodulin, mimecan), non-collagenous glycoproteins (fibronectin, vitronectin, laminin), extracellular matrix proteins (thrombospondins 1-4, dystroglycan, transgelins 1-3), and integrins alpha-V, alpha-1 and 2, beta-1, -2, and -5. Twenty-one unambiguous alternative splicing protein isoforms were identified in optic nerve and ten unambiguous alternative splicing protein isoforms were identified in sclera. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD001581.
authors.affiliation: Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Pingbo
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Rabia
authors.initials: R
authors.lastname: Karani
authors.affiliation: Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Randi L
authors.initials: RL
authors.lastname: Turner
authors.affiliation: Thermo Fisher Scientific, West Palm Beach, FL, USA.
authors.forename: Craig
authors.initials: C
authors.lastname: Dufresne
authors.affiliation: Rochester Eye Associates, Rochester, NY, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Ferri
authors.affiliation: Advanced Clinical BioSystems Research Institute, the Heart Institute and Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Van Eyk
authors.affiliation: Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. rdsemba@jhmi.edu.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Semba
mesh_terms: D002853:Chromatography, Liquid; D005123:Eye / Q000378:metabolism; D006801:Humans; D009900:Optic Nerve / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D012590:Sclera / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome
keywords: Biomedicine; Eye; Human; Optic nerve; Sclera