title: Characterization of integrons and novel cassette arrays in bacteria from clinical isloates in China, 2000-2014.
abstract: Rapid dissemination of antibiotic resistance genes among bacterial isolates is an increasing problem in China. Integron, a conserved DNA sequence, which is carried on episomal genetic structures, plays a very important role in development of antibiotic resistance. This systematic analysis was based on MEDLINE and EMBASE databases. We summarized the distribution and proportion of different types of gene cassette arrays of integrons (including class 1, 2, 3 and atypical class 1 integron) from clinical bacteria isolates in China. Fifty-six literatures were included in this study. Most of the strains were Gram-negative bacteria (94.1%, 7,364/7,822) while only 5.9% strains were Gram-positive bacteria. Class 1 integrons were detected in 54.2% (3956/7295) Gram-negative strains. aadA2 was the most popular gene cassette array detected from 60 Gram-positive bacteria while dfrA17-aadA5 were detected in 426 Gram-negative bacteria. This study identified 12 novel gene cassette arrays which have not been previously found in any species. All the novel gene cassette arrays were detected from Gram-negative bacteria. A regional characteristic of distribution of integrons was presented in this study. The results highlight a need for continuous surveillance of integrons and provide a guide for future research on integron-mediated bacteria resistance.
authors.affiliation: Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.|National Key Clinical Department of Laboratory Medicine, Nanjing, Jiangsu 210029, China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Xia
authors.affiliation: Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.|National Key Clinical Department of Laboratory Medicine, Nanjing, Jiangsu 210029, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
authors.affiliation: Medical Technology Institute of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Qin
authors.affiliation: Medical Technology Institute of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Li
authors.affiliation: Medical Technology Institute of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Haiquan
authors.initials: H
authors.lastname: Kang
authors.affiliation: Medical Technology Institute of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.|Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China; gb20031129@163.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Gu
authors.affiliation: Medical Technology Institute of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.|Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ma
keywords: China; clinical bacteria; integron; novel cassette arrays