title: Malignant Glioma In Vitro Models: On the Utilization of Stem-like Cells.
abstract: Recent publications on the molecular characterization of malignant glioma have had profound impact on the appreciation of tumoral heterogeneity within and between patients. Both these phenomena are implicated in the variability in clinical outcome between patients, as well as the inevitable recurrence of these tumors after conventional treatment. The advent of selective cell culture protocols for the propagation of patient-derived glioma stem-like cells (GSCs) provides researchers the ability to selectively study the cells that could be at the root of tumor proliferation and resistance to therapy. As these techniques are widely applied in contemporary studies and becoming the preferred in vitro model, molecular characterization of GSCs is considered pivotal for the identification and advancement of novel therapies for this devastating disease. This review aims to provide an overview of canonical molecular alterations defining subtypes of malignant glioma as derived from genotypic, transcriptomic and epigenetic profiling in relation to their representation in GSC models. The distribution of these hallmark alterations as found in characterization studies of GSCs is compared between publications. Finally, conclusions of these studies with respect to coverage of driving alterations and translational relevance are provided. By doing so, we provide a contemporary overview of scientific results derived from GSC models and hopefully create appreciation of the advantages and caveats of utilizing these models for studying malignant glioma.
authors.affiliation: Department of Neurosurgery, Brain Tumor Center, Erasmus Medical Center, Rotterdam, Netherlands.
authors.forename: Rutger K
authors.initials: RK
authors.lastname: Balvers
authors.forename: Clemens M F
authors.initials: CM
authors.lastname: Dirven
authors.forename: Sieger
authors.initials: S
authors.lastname: Leenstra
authors.forename: Martine L M
authors.initials: ML
authors.lastname: Lamfers
mesh_terms: D001706:Biopsy; D018929:Cell Culture Techniques / Q000379:methods*; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D005910:Glioma / Q000473:pathology*; D006801:Humans; D008745:Methylation; D014411:Neoplastic Stem Cells / Q000473:pathology; D014407:Tumor Cells, Cultured
keywords: GSC; Glioblastoma; TCGA; cell culture; in vitro models; tumor heterogeneity; tumor profiling