title: Interactive protein network of FXIII-A1 in lipid rafts of activated and non-activated platelets.
abstract: Lipid-rafts are defined as membrane microdomains enriched in cholesterol and glycosphingolipids within platelet plasma membrane. Lipid raft-mediated clot retraction requires factor XIII and other interacting proteins. The aim of this study was to investigate the proteins that interact with factor XIII in raft and non-raft domains of activated and non-activated platelet plasma membrane. By lipidomics analysis, we identified cholesterol- and sphingomyelin-enriched areas as lipid rafts. Platelets were activated by thrombin. Proteomics analysis provided an overview of the pathways in which proteins of rafts and non-rafts participated in the interaction network of FXIII-A1, a catalytic subunit of FXIII. "Platelet activation" was the principal pathway among KEGG pathways for proteins of rafts, both before and after activation. Network analysis showed four types of interactions (activation, binding, reaction, and catalysis) in raft and non-raft domains in interactive network of FXIII-A1. FXIII-A1 interactions with other proteins in raft domains and their role in homeostasis highlight the specialization of the raft domain in clot retraction via the Factor XIII protein network.
authors.affiliation: a EA 3920 - Université de Franche Comté , Besançon , France.
authors.forename: Vahideh
authors.initials: V
authors.lastname: Rabani
authors.affiliation: a EA 3920 - Université de Franche Comté , Besançon , France.|b Laboratoire de Pharmacologie Clinique et Toxicologie , CHU de Besançon , France.
authors.forename: Damien
authors.initials: D
authors.lastname: Montange
authors.affiliation: a EA 3920 - Université de Franche Comté , Besançon , France.|b Laboratoire de Pharmacologie Clinique et Toxicologie , CHU de Besançon , France.
authors.forename: Siamak
authors.initials: S
authors.lastname: Davani
mesh_terms: D001792:Blood Platelets / Q000378:metabolism*; D003018:Clot Retraction; D005176:Factor XIII / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D021962:Membrane Microdomains / Q000378:metabolism*; D015539:Platelet Activation; D011485:Protein Binding; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D021122:Protein Subunits / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction
chemical_list: D021122:Protein Subunits; D020543:Proteome; D005176:Factor XIII
keywords: Clot retraction; cholesterol; factor XIII; lipid rafts; platelets