title: Profiling Metal Oxides with Lipids: Magnetic Liposomal Nanoparticles Displaying DNA and Proteins.
abstract: Metal oxides include many important materials with various surface properties. For biomedical and analytical applications, it is desirable to engineer their biocompatible interfaces. Herein, a phosphocholine liposome (DOPC) and its headgroup dipole flipped counterpart (DOCP) were mixed with ten common oxides. Using the calcein leakage assay, cryo-TEM, and ζ-potential measurement, these oxides were grouped into three types. The type 1 oxides (Fe3 O4 , TiO2 , ZrO2 , Y2 O3 , ITO, In2 O3 , and Mn2 O3 ) form supported bilayers only with DOCP. Type 2 (SiO2 ) forms supported bilayers only with DOPC; type 3 (ZnO and NiO) are cationic and damage lipid membranes. Magnetic Fe3 O4 nanoparticles were further studied for conjugation of fluorophores, proteins, and DNA to the supported DOCP bilayers via lipid headgroup labeling, covalent linking, or lipid insertion. Delivery of the conjugates to cells and selective DNA hybridization were demonstrated. This work provides a general solution for coating the type 1 oxides with a simple mixing in water, facilitating applications in biosensing, separation, and nanomedicine.
authors.affiliation: School of Biological and Medical Engineering, Hefei University of Technology, Hefei, Anhui, 230009, China.|Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
authors.forename: Yibo
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
authors.forename: Zhi Yuan William
authors.initials: ZY
authors.lastname: Lin
authors.affiliation: Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
authors.forename: Biwu
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada. liujw@uwaterloo.ca.
authors.forename: Juewen
authors.identifier: 0000-0001-5918-9336
authors.initials: J
authors.lastname: Liu
mesh_terms: D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D011506:Proteins / Q000008:administration & dosage* / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D058185:Magnetite Nanoparticles; D008670:Metals; D010087:Oxides; D010713:Phosphatidylcholines; D011506:Proteins; D012822:Silicon Dioxide; D004247:DNA; C017251:1,2-oleoylphosphatidylcholine
keywords: liposomes; magnetic nanoparticles; metal oxides; phosphocholine; supported bilayers