title: Temporal Change of Alcian Blue-Stained Primo Vascular System in Lymph Vessels of Rats.
abstract: This study aims to investigate the temporal change of a vascular system now known as the primo vascular system (PVS). We used Alcian blue (AB) dye for imaging the distribution of the PVS in lymphatic vessels. The target lymph vessels were chosen as they are easily accessible from the skin, and long-term observation is possible with intact physiological conditions due to a minimal surgical procedure. AB solution was injected into the inguinal lymph node and the target lymph vessels were located along the superficial epigastric vessels. The imaging system allowed processing for extraction of images showing changes in the AB intensity of the visualized PVS components. This newly developed procedure can be used for further study on various dynamic processes of PVS in lymph vessels.
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon, 443-270, South Korea.
authors.forename: Jungdae
authors.initials: J
authors.lastname: Kim
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon, 443-270, South Korea.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon, 443-270, South Korea.|Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, Suwon, 443-270, South Korea.
authors.forename: Sharon Jiyoon
authors.initials: SJ
authors.lastname: Jung
authors.affiliation: Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon, 443-270, South Korea. kssoh1@gmail.com.
authors.forename: Kwang-Sup
authors.initials: KS
authors.lastname: Soh
mesh_terms: D015670:Acupuncture Therapy / Q000379:methods*; D000423:Alcian Blue / Q000008:administration & dosage*; D000818:Animals; D004396:Coloring Agents / Q000008:administration & dosage*; D007267:Injections; D042601:Lymphatic Vessels / Q000033:anatomy & histology*; D008297:Male; D016740:Meridians; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D000423:Alcian Blue
keywords: Acupuncture; Alcian blue; Lymph; Meridians; Primo vascular system