title: Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements.
abstract: Insertion mutagenesis using known DNA sequences such as T-DNA and transposons is an important tool for studies on gene function in plant sciences. The transposons Activator (Ac)/Dissociation (Ds) have been systematically used to manipulate plant chromosomes. For both of these applications, the recovery of genomic DNA sequences flanking the insertions is required to estimate the sizes and/or scales of the reconstituted chromosomes. In this chapter, we describe the protocols for thermal asymmetric interlaced PCR (TAIL-PCR) for isolation of genomic sequences flanking DNA inserts in plant genomes.
authors.affiliation: Institute of Plant Science and Resources, Okayama University, 2-20-1, Chuo, Kurashiki, 710-0046, Japan.|Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641, Yamazaki, Noda, Chiba, 278-8510, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Fujimoto
authors.affiliation: Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641, Yamazaki, Noda, Chiba, 278-8510, Japan.
authors.forename: Sachihiro
authors.initials: S
authors.lastname: Matsunaga
authors.affiliation: Institute of Plant Science and Bioresources, Okayama University, 2-20-1, Chuo, Kurashiki, 710-0046, Japan. mmura@okayama-u.ac.jp.
authors.forename: Minoru
authors.initials: M
authors.lastname: Murata
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D018745:Genome, Plant; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C036483:T-DNA
keywords: Ac/Ds; Flanking sequence; T-DNA; TAIL-PCR; Transposon