title: Proteomic identification of cyclophilin A as a potential biomarker and therapeutic target in oral submucous fibrosis.
abstract: Oral submucous fibrosis (OSF) is a pre-cancerous lesion, which is characterized by fibrosis of the oral submucosa. Despite large body of studies focusing on this disease, the molecular mechanisms underlying the progression of OSF remained unclear. In this study, 2-DE-based proteomic approaches were employed to identify the differently expressed proteins between OSF and normal tissues. In total, 88 proteins were identified with altered expression levels, including CypA. Upregulation of CypA was further validated through immunohistochemistry staining combined with Q-PCR and western blot by using clinical samples. Statistical analyses reveal that CypA expression level is correlated to the progression of OSF. Finally, functional study reveals a pro-proliferative property of CypA in fibroblast cells by using multiple in vitro models. The present data suggest that CypA might be a potential biomarker and therapeutic target for OSF, and will lead to a better understanding of OSF pathogenesis.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu China, 610041.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Laboratory of Oral Biomedical Science and Translational Medicine, School of Stomatology, Tongji University, Shanghai China, 200072.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Hou
authors.affiliation: Xiangya Stomatological Hospital, Central South University, Changsha, China, 410008.
authors.forename: Hui
authors.initials: H
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu China, 610041.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu China, 610041.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu China, 610041.
authors.forename: Wang
authors.initials: W
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu China, 610041.
authors.forename: Jing
authors.initials: J
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu China, 610041.
authors.forename: Chongkui
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Stomatology, Second Xiangya Hospital, Central South University, Changsha, China, 410008.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Center of Stomatology, Xiangya Hospital, Central South University, Changsha, China, 410008.
authors.forename: Jieying
authors.initials: J
authors.lastname: Peng
authors.affiliation: Center of Stomatology, Xiangya Hospital, Central South University, Changsha, China, 410008.
authors.forename: Yingfang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Center of Stomatology, Xiangya Hospital, Central South University, Changsha, China, 410008.
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Center of Stomatology, Xiangya Hospital, Central South University, Changsha, China, 410008.
authors.forename: Changyun
authors.initials: C
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu China, 610041.
authors.forename: Qianming
authors.initials: Q
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D021984:Cyclophilin A / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism; D009914:Oral Submucous Fibrosis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D034622:RNA Interference; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D021984:Cyclophilin A
keywords: CypA; fibroblast; oral submucous fibrosis; proteomics