title: Abnormal expression of inflammatory genes in placentas of women with sickle cell anemia and sickle hemoglobin C disease.
abstract: Sickle cell disease (SCD) is a complex disease that is characterized by the polymerization of deoxyhemoglobin S, altered red blood cell membrane biology, endothelial activation, hemolysis, a procoagulant state, acute and chronic inflammation, and vaso-occlusion. Among the physiological changes that occur during pregnancy, oxygen is consumed by fetal growth, and pregnant women with SCD are more frequently exposed to low oxygen levels. This might lead to red blood cells sickling, and, consequently, to vaso-occlusion. The mechanisms by which SCD affects placental physiology are largely unknown, and chronic inflammation might be involved in this process. This study aimed to evaluate the gene expression profile of inflammatory response mediators in the placentas of pregnant women with sickle cell cell anemia (HbSS) and hemoglobinopathy SC (HbSC). Our results show differences in a number of these genes. For the HbSS group, when compared to the control group, the following genes showed differential expression: IL1RAP (2.76-fold), BCL6 (4.49-fold), CXCL10 (-2.12-fold), CXCR1 (-3.66-fold), and C3 (-2.0-fold). On the other hand, the HbSC group presented differential expressions of the following genes, when compared to the control group: IL1RAP (4.33-fold), CXCL1 (3.05-fold), BCL6 (4.13-fold), CXCL10 (-3.32-fold), C3 (-2.0-fold), and TLR3 (2.38-fold). Taken together, these data strongly suggest a differential expression of several inflammatory genes in both SCD (HbSS and HbSC), indicating that the placenta might become an environment with hypoxia, and increased inflammation, which could lead to improper placental development.
authors.affiliation: Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: Letícia C
authors.initials: LC
authors.lastname: Baptista
authors.affiliation: Department of Obstetrics and Gynecology, University of Campinas - UNICAMP, School of Medicine, Campinas, São Paulo, Brazil.
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Costa
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.
authors.forename: Regiane
authors.initials: R
authors.lastname: Ferreira
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.
authors.forename: Dulcinéia M
authors.initials: DM
authors.lastname: Albuquerque
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Lanaro
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.
authors.forename: Kleber Y
authors.initials: KY
authors.lastname: Fertrin
authors.affiliation: Department of Obstetrics and Gynecology, University of Campinas - UNICAMP, School of Medicine, Campinas, São Paulo, Brazil.
authors.forename: Fernanda G
authors.initials: FG
authors.lastname: Surita
authors.affiliation: Department of Obstetrics and Gynecology, University of Campinas - UNICAMP, School of Medicine, Campinas, São Paulo, Brazil.
authors.forename: Mary A
authors.initials: MA
authors.lastname: Parpinelli
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.
authors.forename: Fernando F
authors.initials: FF
authors.lastname: Costa
authors.affiliation: Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas - UNICAMP, Campinas, SP, Brazil. melomb@uol.com.br.|Laboratory of Human Genetics, Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas - UNICAMP, P.O. Box: 6010, Campinas, SP, 13083-875, Brazil. melomb@uol.com.br.
authors.forename: Mônica Barbosa de
authors.initials: MB
authors.lastname: Melo
mesh_terms: D000328:Adult; D000755:Anemia, Sickle Cell / Q000150:complications / Q000235:genetics*; D016022:Case-Control Studies; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006450:Hemoglobin SC Disease / Q000150:complications / Q000235:genetics*; D006801:Humans; D007249:Inflammation / Q000209:etiology / Q000235:genetics*; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D011250:Pregnancy Complications, Hematologic / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D018121:Receptors, Cytokine / Q000096:biosynthesis* / Q000235:genetics; D017584:Reproductive History; D055815:Young Adult
chemical_list: D016207:Cytokines; D018121:Receptors, Cytokine
keywords: Gene expression; Inflammation; Placenta; Pregnancy; Sickle cell disease