title: A Dipolar Anthracene Dye: Synthesis, Optical Properties and Two-photon Tissue Imaging.
abstract: Two-photon microscopy is a powerful tool for studying biological systems. In search of novel two-photon absorbing dyes for bioimaging, we synthesized a new anthracene-based dipolar dye (anthradan) and evaluated its two-photon absorbing and imaging properties. The new anthradan, 9,10-bis(o-dimethoxy-phenyl)-anthradan, absorbs and emits at longer wavelengths than acedan, a well-known two-photon absorbing dye. It is also stable under two-photon excitation conditions and biocompatible, and thus used for two-photon imaging of mouse organ tissues to show bright, near-red fluorescence along with negligible autofluorescence. Such an anthradan thus holds promise as a new class of two-photon absorbing dyes for the development of fluorescent probes and tags for biological systems.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk, 37673, Republic of Korea.
authors.forename: Hyunsoo
authors.initials: H
authors.lastname: Moon
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk, 37673, Republic of Korea.
authors.forename: Yong Woong
authors.initials: YW
authors.lastname: Jun
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk, 37673, Republic of Korea. dok036@ucsd.edu.|Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA. dok036@ucsd.edu.
authors.forename: Dokyoung
authors.identifier: http://orcid.org/0000-0002-7756-3560
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk, 37673, Republic of Korea.
authors.forename: Hye Gun
authors.initials: HG
authors.lastname: Ryu
authors.affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk, 37673, Republic of Korea.
authors.forename: Taejun
authors.initials: T
authors.lastname: Wang
authors.affiliation: Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk, 37673, Republic of Korea.
authors.forename: Ki Hean
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Anatomy and Neurobiology, Kyung Hee University, School of Medicine, Biomedical Science Institute, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul, 130-701, Republic of Korea.
authors.forename: Youngbuhm
authors.initials: Y
authors.lastname: Huh
authors.affiliation: Department of Anatomy and Neurobiology, Kyung Hee University, School of Medicine, Biomedical Science Institute, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul, 130-701, Republic of Korea.
authors.forename: Junyang
authors.initials: J
authors.lastname: Jung
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk, 37673, Republic of Korea. ahn@postech.ac.kr.
authors.forename: Kyo Han
authors.initials: KH
authors.lastname: Ahn
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000138:chemical synthesis* / Q000737:chemistry*; D001921:Brain / Q000000981:diagnostic imaging; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D007668:Kidney / Q000000981:diagnostic imaging; D008099:Liver / Q000000981:diagnostic imaging; D008168:Lung / Q000000981:diagnostic imaging; D051379:Mice; D015394:Molecular Structure; D061848:Optical Imaging; D055620:Optical Phenomena; D017785:Photons; D011789:Quantum Theory; D013154:Spleen / Q000000981:diagnostic imaging
chemical_list: D000873:Anthracenes; D005456:Fluorescent Dyes; C034020:anthracene
keywords: anthracene; bioimaging; dipolar dyes; red-emitting dyes; two-photon