title: Genetics and molecular mapping of resistance to Plasmodiophora brassicae pathotypes 2, 3, 5, 6, and 8 in rutabaga (Brassica napus var. napobrassica).
abstract: Clubroot disease, caused by Plasmodiophora brassicae, is a threat to the production of Brassica crops including oilseed B. napus. In Canada, several pathotypes of this pathogen, such as pathotypes 2, 3, 5, 6, and 8, were identified, and resistance to these pathotypes was found in a rutabaga (B. napus var. napobrassica) genotype. In this paper, we report the genetic basis and molecular mapping of this resistance by use of F2, backcross (BC1), and doubled haploid (DH) populations generated from crossing of this rutabaga line to a susceptible spring B. napus canola line. The F1, F2, and BC1populations were evaluated for resistance to pathotype 3, and the DH population was evaluated for resistance to pathotypes 2, 3, 5, 6, and 8. A 3:1 segregation in F2and a 1:1 segregation in BC1were found for resistance to pathotype 3, and a 1:1 segregation was found in the DH population for resistance to all pathotypes. Molecular mapping by using the DH population identified a genomic region on chromosome A8 carrying resistance to all five pathotypes. This suggests that a single gene or a cluster of genes, located in this genomic region, is involved in the control of resistance to these pathotypes.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, 4-10 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5, Canada.|Department of Agricultural, Food and Nutritional Science, University of Alberta, 4-10 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5, Canada.
authors.forename: Muhammad Jakir
authors.initials: MJ
authors.lastname: Hasan
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, 4-10 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5, Canada.|Department of Agricultural, Food and Nutritional Science, University of Alberta, 4-10 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5, Canada.
authors.forename: Habibur
authors.initials: H
authors.lastname: Rahman
mesh_terms: D029688:Brassica napus / Q000235:genetics* / Q000469:parasitology*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D056726:Genetic Association Studies; D008040:Genetic Linkage; D005819:Genetic Markers; D006238:Haploidy; D018895:Microsatellite Repeats; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology*; D056909:Plasmodiophorida / Q000235:genetics / Q000302:isolation & purification; D040641:Quantitative Trait Loci
chemical_list: D005819:Genetic Markers
keywords: Brassica napus; canola; cartographie; clubroot resistance; colza; mapping; rutabaga; résistance à la hernie des crucifères