title: Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration.
abstract: In this study, bioactive scaffold of nano magnesium phosphate (nMP)/wheat protein (WP) composite (MWC) was fabricated. The results revealed that the MWC scaffolds had interconnected not only macropores (sized 400-600 μm) but also micropores (sized 10-20 μm) on the walls of macropores. The MWC scaffolds containing 40 w% nMP had an appropriate degradability in phosphate-buffered saline and produced a weak alkaline microenvironment. In cell culture experiments, the results revealed that the MWC scaffolds significantly promoted the MC3T3-E1 cell proliferation, differentiation, and growth into the scaffolds. The results of synchrotron radiation microcomputed tomography and analysis of the histological sections of the in vivo implantation revealed that the MWC scaffolds evidently improved the new bone formation and bone defects repair as compared with WP scaffolds. Moreover, it was found that newly formed bone tissue continued to increase with the gradual reduction of materials residual in the MWC scaffolds. Furthermore, the immunohistochemical analysis further offered the evidence of the stimulatory effects of MWC scaffolds on osteogenic-related cell differentiation and new bone regeneration. The results indicated that MWC scaffolds with good biocompability and degradability could promote osteogenesis in vivo, which would have potential for bone tissue repair.
authors.affiliation: Department of Emergency.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Department of Emergency.
authors.forename: Panyu
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, Changhai Hospital, Second Military Medical University.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Changhai Hospital, Second Military Medical University.
authors.forename: Chao
authors.initials: C
authors.lastname: Qiu
authors.affiliation: Department of Emergency.
authors.forename: Panfeng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Emergency.
authors.forename: Yuntong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People's Republic of China.
authors.forename: Liming
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Emergency; Department of Orthopedics, Changhai Hospital, Second Military Medical University.
authors.forename: Shuogui
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005269:Femur / Q000166:cytology; D017616:Magnesium Compounds / Q000737:chemistry; D008297:Male; D008422:Materials Testing; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D010012:Osteogenesis / Q000502:physiology*; D010710:Phosphates / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D011817:Rabbits; D054457:Tissue Scaffolds / Q000737:chemistry; D014908:Triticum / Q000737:chemistry*; D055114:X-Ray Microtomography
chemical_list: D017616:Magnesium Compounds; D010710:Phosphates; D010940:Plant Proteins; C030781:magnesium phosphate
keywords: bone regeneration; composite scaffolds; degradation; nano magnesium phosphate; wheat protein