title: Quantification of Bacterial Fatty Acids by Extraction and Methylation.
abstract: This protocol describes two similar methods for the extraction and methylation of fatty acids from bacterial cultures. The acid derivatization protocol (Lennenet al., 2013; Bligh and Dyer, 1959) results in the extraction and methylation of all fatty acids, both free and bound, from a bacterial culture, while the base derivatization protocol (Lennen and Pfleger, 2013) captures only bound (phospholipid, acyl-thioester) species. After extraction into hexane, the lipids may be analyzed by gas chromatography.
authors.affiliation: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Politz
authors.affiliation: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Lennen
authors.affiliation: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Pfleger