title: Obtaining More Accurate Signals: Spatiotemporal Imaging of Cancer Sites Enabled by a Photoactivatable Aptamer-Based Strategy.
abstract: Early cancer diagnosis is of great significance to relative cancer prevention and clinical therapy, and it is crucial to efficiently recognize cancerous tumor sites at the molecular level. Herein, we proposed a versatile and efficient strategy based on aptamer recognition and photoactivation imaging for cancer diagnosis. This is the first time that a visible light-controlled photoactivatable aptamer-based platform has been applied for cancer diagnosis. The photoactivatable aptamer-based strategy can accurately detect nucleolin-overexpressed tumor cells and can be used for highly selective cancer cell screening and tissue imaging. This strategy is available for both formalin-fixed paraffin-embedded tissue specimens and frozen sections. Moreover, the photoactivation techniques showed great progress in more accurate and persistent imaging to the use of traditional fluorophores. Significantly, the application of this strategy can produce the same accurate results in tissue specimen analysis as with classical hematoxylin-eosin staining and immunohistochemical technology.
authors.affiliation: Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430022, P.R. China.
authors.forename: Heng
authors.initials: H
authors.lastname: Xiao
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Chen
authors.forename: Erfeng
authors.initials: E
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Zhuoran
authors.initials: Z
authors.lastname: Jiang
authors.forename: Lai
authors.initials: L
authors.lastname: Wei
authors.forename: Haomiao
authors.initials: H
authors.lastname: Su
authors.forename: Weiwu
authors.initials: W
authors.lastname: Zeng
authors.forename: Yunjiu
authors.initials: Y
authors.lastname: Gan
authors.forename: Zijing
authors.initials: Z
authors.lastname: Wang
authors.forename: Bifeng
authors.initials: B
authors.lastname: Yuan
authors.forename: Shanshan
authors.initials: S
authors.lastname: Qin
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Leng
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Songmei
authors.initials: S
authors.lastname: Liu
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D006801:Humans; D009369:Neoplasms; D013194:Staining and Labeling
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes
keywords: aptamer; breast cancer; cancer site; fluorescent imaging; nucleolin; photoactivation