title: SIRT1 was involved in TNF-α-promoted osteogenic differentiation of human DPSCs through Wnt/β-catenin signal.
abstract: Dental pulp stem cells (DPSCs), as one type of mesenchymal stem cells (MSCs), have the capability of self-renewal and differentiating along the various directions, including osteogenic, chondrogenic, neurogenic, and adipogenic. We previously study and found that tumor necrosis factor-α (TNF-α) promoted osteogenic differentiation of human DPSCs via the Wnt/β-catenin signaling pathway in low concentration while inhibited that in high concentration. In the abovementioned process, we found that sirtuin-1 (SIRT1) had the same change compared with the characteristic protein of bone formation, such as bone morphogenetic protein 2 (BMP2), runt-related transcription factor 2 (Runx2), and collagen I (COL1). We asked whether SIRT1 could regulate osteogenesis of DPSCs. In inflammation microenvironment constructed by TNF-α, we tested the expression changing of SIRT1 and analyzed the function of SIRT1 on osteogenic differentiation of DPSCs. SIRT1 deacetylated β-catenin, and then promote its accumulation in the nucleus. Accumulated β-catenin can lead to transcription of osteogenic characteristic genes. Using the activator of SIRT1, resveratrol, could promote the above-mentioned process of osteogenic differentiation. SIRT1 could regulate osteogenesis of DPSCs through Wnt/β-catenin signal. SIRT1, as a regulator of differentiation of DPSCs, may be a new target for cell-based therapy in oral diseases and other regenerative medicine.
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, China.
authors.forename: Guijuan
authors.initials: G
authors.lastname: Feng
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Song
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Cao
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Shen
authors.affiliation: Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong, 226001, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, China. fengxingmeint@126.com.
authors.forename: Xingmei
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Pathogen Biology, Medical College, Nantong University, Nantong, 226001, China. zdm060808@aliyun.com.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000293:Adolescent; D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects*; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects*; D056564:Sirtuin 1 / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D055815:Young Adult; D051176:beta Catenin / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha; D051176:beta Catenin; D056564:Sirtuin 1
keywords: DPSCs; Osteogenic differentiation; SIRT1; Wnt/β-catenin