title: The role of long non-coding RNA HOTAIR in the progression and development of laryngeal squamous cell carcinoma interacting with EZH2.
abstract: CONCLUSIONS
  The aberrant expression of long non-coding RNA HOTAIR and transmethylase EZH2 played important roles in the progression and development of laryngeal squamous cell carcinoma (LSCC).
  
  
  OBJECTIVE
  This research was aimed to explore the expression and correlation with clinicopathological characteristics of HOTAIR and EZH2 in LSCC, and to evaluate the function of the two in regulating the proliferation and cis-platinum resistance processes of LSCC.
  
  
  METHODS
  Quantitative real time-PCR (qPCR) was conducted to measure the expression of HOTAIR and EZH2 in tissue samples. Clinicopathological features were collected and statistically analyzed combining with the expression of HOTAIR and EZH2. The variance of EZH2 with down-regulating HOTAIR was measured by qPCR. CCK-8 proliferation test was conducted to detect the proliferation feature in LSCC cells. After cultured with a series of cis-platinum concentrations for 24 h, cell viability was detected using CCK-8 assay, and the inhibition rates were calculated.
  
  
  RESULTS
  HOTAIR and EZH2 were over-expressed in LSCC tissue. The higher expression was significantly related to T phase, pathological grades, and risk of lymphatic metastasis of LSCC. Suppressing HOTAIR expression stimulated EZH2 expressing, promoted the proliferation of AMC-HN8 cells, and increased the sensitivity to cis-platinum of the LSCC cells.
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Shanghai Key Clinical Disciplines of Otorhinolaryngology, Eye, Ear, Nose & Throat Hospital, Fudan University , Shanghai , PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zheng
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Shanghai Key Clinical Disciplines of Otorhinolaryngology, Eye, Ear, Nose & Throat Hospital, Fudan University , Shanghai , PR China.
authors.forename: Xiyan
authors.initials: X
authors.lastname: Xiao
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Shanghai Key Clinical Disciplines of Otorhinolaryngology, Eye, Ear, Nose & Throat Hospital, Fudan University , Shanghai , PR China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Wu
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Shanghai Key Clinical Disciplines of Otorhinolaryngology, Eye, Ear, Nose & Throat Hospital, Fudan University , Shanghai , PR China.
authors.forename: Jiameng
authors.initials: J
authors.lastname: Huang
authors.affiliation: b Laboratory of Eye, Ear, Nose & Throat Hospital , Fudan University , Shanghai , PR China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Shanghai Key Clinical Disciplines of Otorhinolaryngology, Eye, Ear, Nose & Throat Hospital, Fudan University , Shanghai , PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Xie
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Shanghai Key Clinical Disciplines of Otorhinolaryngology, Eye, Ear, Nose & Throat Hospital, Fudan University , Shanghai , PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: a Department of Otorhinolaryngology Head and Neck Surgery , Shanghai Key Clinical Disciplines of Otorhinolaryngology, Eye, Ear, Nose & Throat Hospital, Fudan University , Shanghai , PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002945:Cisplatin / Q000627:therapeutic use; D018450:Disease Progression; D019008:Drug Resistance, Neoplasm; D000071221:Enhancer of Zeste Homolog 2 Protein / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D062085:RNA, Long Noncoding / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; C561566:HOTAIR long untranslated RNA, human; D062085:RNA, Long Noncoding; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D002945:Cisplatin
keywords: Clinicopathological characteristics; cis-platinum resistance; down-regulation; inconsistence; proliferation