title: Laser-induced heating integrated with a microfluidic platform for real-time DNA replication and detection.
abstract: This study developed a microfluidic platform for replicating and detecting DNA in real time by integrating a laser and a microfluidic device composed of polydimethylsiloxane. The design of the microchannels consisted of a laser-heating area and a detection area. An infrared laser was used as the heating source for DNA replication, and the laser power was adjusted to heat the solutions directly. In addition, strong biotin–avidin binding was used to capture and detect the replicated products. The biotin on one end was bound to avidin and anchored to the surface of the microchannels, whereas the biotin on the other end was bound to the quantum dots (Qdots). The results showed that the fluorescent intensity of the Qdots bound to the replicated products in the detection area increased with the number of thermal cycles created by the laser. When the number of thermal cycles was ≥10, the fluorescent intensity of the Qdots was directly detectable on the surface of the microchannels. The proposed method is more sensitive than detection methods entailing gel electrophoresis.
authors.affiliation: National Chiayi University, Department of Biomechatronic Engineering, No. 300 Syuefu Road, Chiayi 60004, Taiwan.
authors.forename: Min-Sheng
authors.initials: MS
authors.lastname: Hung
authors.affiliation: Chip Win Technology Co., Ltd., Biomedical and Advanced Systems Integration, No. 18 Sec. 2, Seng Yi Road, Zhubei City, Hsinchu 30261, Taiwan.
authors.forename: Chia-Chin
authors.initials: CC
authors.lastname: Ho
authors.affiliation: National Chiayi University, Department of Biomechatronic Engineering, No. 300 Syuefu Road, Chiayi 60004, Taiwan.
authors.forename: Chih-Pin
authors.initials: CP
authors.lastname: Chen
mesh_terms: D001360:Avidin; D004247:DNA / Q000032:analysis*; D004261:DNA Replication; D006361:Heating; D007834:Lasers; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D045663:Quantum Dots
chemical_list: D001360:Avidin; D004247:DNA