title: Hyperacetylation of Microtubules in Mesenchymal Cells Increases Cytokeratin 14-Positive Epithelial Progenitors in Developing Salivary Glands.
abstract: Cells engage in bidirectional communication with their surroundings. This reciprocal dialogue between cells and their cellular microenvironments often governs the maintenance and differentiation of stem/progenitor cells. Here, the authors present evidence that in developing salivary gland explants, a single posttranslational change in microtubules in mesenchymal cells alters the mesenchymal microenvironment and promotes the maintenance and differentiation of a subset of epithelial progenitor cells that impairs branching morphogenesis. Specifically, the authors report that hyperacetylation of microtubules in mesenchymal cells increased cytokeratin 14-positive (K14+) progenitors and their differentiated progeny, myoepithelial cells, in epithelial basal and suprabasal layers in the distal endbud region of developing salivary glands. Mechanistically, this process engages the transforming growth factor β1 protein and Notch signaling pathways. This report establishes that a simple posttranslational change in the cytoskeletal system of mesenchyme dictates the maintenance and differentiation of adjacent epithelial progenitor cells to alter branching morphogenesis of the epithelium.
authors.affiliation: Laboratory of Cell and Developmental Biology, Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.
authors.forename: E E
authors.initials: EE
authors.lastname: Joo
authors.affiliation: Laboratory of Cell and Developmental Biology, Matrix and Morphogenesis Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.
authors.forename: I M A
authors.initials: IM
authors.lastname: Lombaert
authors.affiliation: Laboratory of Cell and Developmental Biology, Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA kenneth.yamada@nih.gov.
authors.forename: K M
authors.initials: KM
authors.lastname: Yamada
mesh_terms: D000107:Acetylation; D000818:Animals; D002450:Cell Communication; D002454:Cell Differentiation; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology; D053547:Keratin-14 / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D009924:Organ Culture Techniques; D051880:Receptors, Notch / Q000378:metabolism; D012469:Salivary Glands / Q000196:embryology*; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D005353:Fibronectins; D053547:Keratin-14; D051880:Receptors, Notch; D016212:Transforming Growth Factor beta; D008345:Manganese
keywords: cell differentiation; cell signaling; epithelial-mesenchymal interaction; extracellular matrix (ECM); morphogenesis; salivary physiology