title: [Effect of Elodea nuttallii-immobilized Nitrogen Cycling Bacteria on Nitrogen Removal Mechanism in an Inflow River, Gonghu Bay].
abstract: Undisturbed sediment cores and surface water from Qinshui River in Gonghu Bay were collected to carry out a simulation experiment in our laboratory. The remediation effect of Elodea nuttallii-Immobilized Nitrogen Cycling Bacteria (INCB) was applied in the polluted inflow river. The denitrification rate, ANAMMOX rate and nitrogen microorganism diversity were measured by ¹⁵N isotope pairing technology and high-throughput sequencing technology based on 16S rRNA. The TN, NH₄⁺-N, NO₃⁻-N concentrations were reduced by 72.03%, 46.67% and 76.65% in the treatment with addition of Elodea nuttallii and INCB in our laboratory experiment. Meanwhile, denitrification bacteria and ANAMMOX bacteria had synergistic effect with each other. The denitrification and ANAMMOX rates were increased by 165 µmol (m² · h)⁻¹ and 269.7 µmol · (m² · h)⁻¹, respectively. The diversities of denitrification and ANAMMOX bacteria also increased in our experiment. From the level of major phylum, Proteobacteria, Planctomycetes, Acidobbacteria and Bacteroidetes all increased significantly. The results showed that the Elodea nuttallii-INCB assemblage technology could increase the bio-diversity of nitrogen cycling bacteria and promote the ability of nitrogen removal in Qinshui River.
authors.forename: Hua-yang
authors.initials: HY
authors.lastname: Han
authors.forename: Zheng-kui
authors.initials: ZK
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D061006:Bays / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D058440:Denitrification; D031321:Hydrocharitaceae / Q000382:microbiology*; D009584:Nitrogen / Q000032:analysis; D058458:Nitrogen Cycle; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000737:chemistry*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D009584:Nitrogen