title: Host metabolic responses to Plasmodium falciparum infections evaluated by1H NMR metabolomics.
abstract: The human malarial parasite Plasmodium falciparum causes the most severe forms of malarial infections, which include cerebral malaria and various organ dysfunctions amongst adults in India. So far no dependable clinical descriptor is available that can distinguish cerebral malaria from other symptomatically similar diseases such as sepsis and encephalitis. This study aims at evaluating the differential metabolic features of plasma samples from P. falciparum patients with varying severities, and patients suffering from symptomatically similar diseases.1H Nuclear Magnetic Resonance (NMR) based metabolic profiling of the plasma of the infected individuals and the control population was performed. The differences in the plasma profiles were evaluated through multivariate statistical analyses. The results suggest malaria-specific elevation of plasma lipoproteins. Such an increase was absent in control populations. In addition, cerebral malaria patients exhibited a decrease in plasma glycoproteins; such a reduction was not observed in malarial patients without cerebral symptoms. The data presented here indicates that the metabolism and/or transport of the plasma lipids is specifically perturbed by malarial infections. The differential perturbation of the plasma glycoprotein levels in cerebral malaria patients may have important implications in the diagnosis of cerebral malaria.
authors.affiliation: University of Pennsylvania, Systems Pharmacology and Translational Therapeutics, Philadelphia, Pennsylvania, USA.
authors.forename: Arjun
authors.initials: A
authors.lastname: Sengupta
authors.affiliation: Tata Institute of Fundamental Research, Department of Chemical Sciences, Homi Bhabha Road, Mumbai, Maharashtra, India. harisonawat@gmail.com.
authors.forename: Soumita
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: SCB Medical College, Department of Medicine, Cuttack, Odhisa, India.
authors.forename: Bidyut K
authors.initials: BK
authors.lastname: Das
authors.affiliation: SCB Medical College, Department of Medicine, Cuttack, Odhisa, India.
authors.forename: Abhinash
authors.initials: A
authors.lastname: Panda
authors.affiliation: SCB Medical College, Department of Biochemistry, Cuttack, Odisha, India.
authors.forename: Rina
authors.initials: R
authors.lastname: Tripathy
authors.affiliation: Centre for Infection and Immunity of Lille, Centre for Infection and Immunity of Lille, Lille, Cedex, France.
authors.forename: Sylviane
authors.initials: S
authors.lastname: Pied
authors.affiliation: Institute of Life Sciences, Bhubaneswar, Odisha 751023, India.
authors.forename: B
authors.initials: B
authors.lastname: Ravindran
authors.affiliation: TIFR, Department of Biological Sciences, Mumbai, Maharashtra, India.
authors.forename: Sulabha
authors.initials: S
authors.lastname: Pathak
authors.affiliation: Tata Institute of Fundamental Research, Department of Chemical Sciences, Homi Bhabha Road, Mumbai, Maharashtra, India. harisonawat@gmail.com.
authors.forename: Shobhona
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Tata Institute of Fundamental Research, Department of Chemical Sciences, Homi Bhabha Road, Mumbai, Maharashtra, India. harisonawat@gmail.com.
authors.forename: Haripalsingh M
authors.initials: HM
authors.lastname: Sonawat
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D004660:Encephalitis / Q000097:blood / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D006790:Host-Parasite Interactions; D006801:Humans; D016778:Malaria, Falciparum / Q000097:blood / Q000378:metabolism* / Q000469:parasitology; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D010963:Plasmodium falciparum; D066244:Proton Magnetic Resonance Spectroscopy; D018805:Sepsis / Q000097:blood / Q000378:metabolism; D055815:Young Adult