title: Enhanced bioadhesivity of dopamine-functionalized polysaccharidic membranes for general surgery applications.
abstract: An emerging strategy to improve adhesiveness of biomaterials in wet conditions takes inspiration from the adhesive features of marine mussel, which reside in the chemical reactivity of catechols. In this work, a catechol-bearing molecule (dopamine) was chemically grafted onto alginate to develop a polysaccharide-based membrane with improved adhesive properties. The dopamine-modified alginates were characterized by NMR, UV spectroscopy and in vitro biocompatibility. Mechanical tests and in vitro adhesion studies pointed out the effects of the grafted dopamine within the membranes. The release of HA from these resorbable membranes was shown to stimulate fibroblasts activities (in vitro). Finally, a preliminary in vivo test was performed to evaluate the adhesiveness of the membrane on porcine intestine (serosa). Overall, this functionalized membrane was shown to be biocompatible and to possess considerable adhesive properties owing to the presence of dopamine residues grafted on the alginate backbone.
  
  
  
  This article describes the development of a mussels-inspired strategy for the development of an adhesive polysaccharide-based membrane for wound healing applications. Bioadhesion was achieved by grafting dopamine moieties on the structural component on the membrane (alginate): this novel biomaterial showed improved adhesiveness to the intestinal tissue, which was demonstrated by both in vitro and in vivo studies. Overall, this study points out how this nature-inspired strategy may be successfully exploited for the development of novel engineered biomaterials with enhanced bioadhesion, thus opening for novel applications in the field of general surgery.
authors.affiliation: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, I-34127 Trieste, Italy. Electronic address: francesca.scognamiglio@phd.units.it.
authors.forename: F
authors.initials: F
authors.lastname: Scognamiglio
authors.affiliation: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, I-34127 Trieste, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Travan
authors.affiliation: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, I-34127 Trieste, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Borgogna
authors.affiliation: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, I-34127 Trieste, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Donati
authors.affiliation: Department of Medical, Surgical and Health Sciences, University of Trieste, Piazza dell'Ospitale 1, I-34129 Trieste, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Marsich
authors.affiliation: Department of Surgery, Research Institute NUTRIM, Maastricht University Medical Centre, Maastricht, Netherlands.
authors.forename: J W A M
authors.initials: JW
authors.lastname: Bosmans
authors.affiliation: RESCOLL Société de Recherche, allée Geoffroy Saint Hilaire 8, F-33615 Pessac, France.
authors.forename: L
authors.initials: L
authors.lastname: Perge
authors.affiliation: RESCOLL Société de Recherche, allée Geoffroy Saint Hilaire 8, F-33615 Pessac, France.
authors.forename: M P
authors.initials: MP
authors.lastname: Foulc
authors.affiliation: Department of Surgery, Research Institute NUTRIM, Maastricht University Medical Centre, Maastricht, Netherlands.
authors.forename: N D
authors.initials: ND
authors.lastname: Bouvy
authors.affiliation: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, I-34127 Trieste, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Paoletti
mesh_terms: D000268:Adhesiveness; D000464:Alginates / Q000138:chemical synthesis; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004298:Dopamine / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D020723:Glucuronic Acid / Q000138:chemical synthesis; D006603:Hexuronic Acids / Q000138:chemical synthesis; D006801:Humans; D055595:Mechanical Phenomena; D008567:Membranes, Artificial; D051379:Mice; D041681:NIH 3T3 Cells; D011134:Polysaccharides / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013514:Surgical Procedures, Operative / Q000379:methods*; D034421:Sus scrofa; D014867:Water; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D011134:Polysaccharides; D014867:Water; D020723:Glucuronic Acid; D004298:Dopamine
keywords: Alginate; Bioadhesion; Dopamine; Functionalized polysaccharides; Membranes