title: Upregulation of an Artificial Zymogen by Proteolysis.
abstract: Regulation of enzymatic activity is vital to living organisms. Here, we report the development and the genetic optimization of an artificial zymogen requiring the action of a natural protease to upregulate its latent asymmetric transfer hydrogenase activity.
authors.affiliation: Department of Chemistry, University of Basel, 4056, Basel, Switzerland.|School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Chemistry, University of Basel, 4056, Basel, Switzerland.
authors.forename: Vincent
authors.initials: V
authors.lastname: Lebrun
authors.affiliation: Department of Chemistry, School of Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
authors.forename: Taku
authors.initials: T
authors.lastname: Kitanosono
authors.affiliation: Department of Chemistry, University of Basel, 4056, Basel, Switzerland.
authors.forename: Hendrik
authors.initials: H
authors.lastname: Mallin
authors.affiliation: Department of Chemistry, University of Basel, 4056, Basel, Switzerland.
authors.forename: Valentin
authors.initials: V
authors.lastname: Köhler
authors.affiliation: Department of Chemistry, University of Basel, 4056, Basel, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Häussinger
authors.affiliation: Laboratory of Organic Chemistry, ETH Zürich, 8093, Zürich, Switzerland.
authors.forename: Donald
authors.initials: D
authors.lastname: Hilvert
authors.affiliation: Department of Chemistry, School of Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
authors.forename: Shu
authors.initials: S
authors.lastname: Kobayashi
authors.affiliation: Department of Chemistry, University of Basel, 4056, Basel, Switzerland. thomas.ward@unibas.ch.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Ward
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism; D002384:Catalysis; D056831:Coordination Complexes / Q000737:chemistry / Q000378:metabolism; D004792:Enzyme Precursors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007495:Iridium / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D059748:Proteolysis; D013379:Substrate Specificity; D015854:Up-Regulation
chemical_list: D056831:Coordination Complexes; D004792:Enzyme Precursors; D010455:Peptides; D007495:Iridium
keywords: artificial metalloenzyme; artificial zymogen; asymmetric catalysis; transfer hydrogenation; upregulation