title: Preparation and optimisation of anionic liposomes for delivery of small peptides and cDNA to human corneal epithelial cells.
abstract: Drug delivery to corneal epithelial cells is challenging due to the intrinsic mechanisms that protect the eye. Here, we report a novel liposomal formulation to encapsulate and deliver a short sequence peptide into human corneal epithelial cells (hTCEpi). Using a mixture of Phosphatidylcholine/Caproylamine/Dioleoylphosphatidylethanolamine (PC/CAP/DOPE), we encapsulated a fluorescent peptide, resulting in anionic liposomes with an average size of 138.8 ± 34 nm and a charge of -18.2 ± 1.3 mV. After 2 h incubation with the peptide-encapsulated liposomes, 66% of corneal epithelial (hTCEpi) cells internalised the FITC-labelled peptide, demonstrating the ability of this formulation to effectively deliver peptide to hTCEpi cells. Additionally, lipoplexes (liposomes complexed with plasmid DNA) were also able to transfect hTCEpi cells, albeit at a modest level (8% of the cells). Here, we describe this novel anionic liposomal formulation intended to enhance the delivery of small cargo molecules in situ.
authors.affiliation: a Department of Pharmacology and Toxicology , University of Louisville , Louisville , KY , USA ;
authors.forename: Luís F
authors.initials: LF
authors.lastname: Neves
authors.affiliation: b Department of Bioengineering , University of Louisville , Louisville , KY , USA ;|c Centre for Predictive Medicine , University of Louisville , Louisville , KY , USA ;
authors.forename: Jinghua
authors.initials: J
authors.lastname: Duan
authors.affiliation: a Department of Pharmacology and Toxicology , University of Louisville , Louisville , KY , USA ;
authors.forename: Adrienne
authors.initials: A
authors.lastname: Voelker
authors.affiliation: d Brown Cancer Centre , University of Louisville , Louisville , KY , USA.
authors.forename: Anil
authors.initials: A
authors.lastname: Khanal
authors.affiliation: d Brown Cancer Centre , University of Louisville , Louisville , KY , USA.
authors.forename: Lacey
authors.initials: L
authors.lastname: McNally
authors.affiliation: a Department of Pharmacology and Toxicology , University of Louisville , Louisville , KY , USA ;|b Department of Bioengineering , University of Louisville , Louisville , KY , USA ;|c Centre for Predictive Medicine , University of Louisville , Louisville , KY , USA ;
authors.forename: Jill
authors.initials: J
authors.lastname: Steinbach-Rankins
authors.affiliation: a Department of Pharmacology and Toxicology , University of Louisville , Louisville , KY , USA ;|d Brown Cancer Centre , University of Louisville , Louisville , KY , USA.
authors.forename: Brian P
authors.initials: BP
authors.lastname: Ceresa
mesh_terms: D002478:Cells, Cultured; D018076:DNA, Complementary / Q000737:chemistry / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D019573:Epithelium, Corneal / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008081:Liposomes; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D010957:Plasmids / Q000737:chemistry / Q000494:pharmacology; D014162:Transfection / Q000379:methods*
chemical_list: D018076:DNA, Complementary; D008081:Liposomes; D010455:Peptides
keywords: Peptide; liposomes; ophthalmic delivery; plasmid DNA