title: Biodegradation and Osteosarcoma Cell Cultivation on Poly(aspartic acid) Based Hydrogels.
abstract: Development of novel biodegradable and biocompatible scaffold materials with optimal characteristics is important for both preclinical and clinical applications. The aim of the present study was to analyze the biodegradability of poly(aspartic acid)-based hydrogels, and to test their usability as scaffolds for MG-63 osteoblast-like cells. Poly(aspartic acid) was fabricated from poly(succinimide) and hydrogels were prepared using natural amines as cross-linkers (diaminobutane and cystamine). Disulfide bridges were cleaved to thiol groups and the polymer backbone was further modified with RGD sequence. Biodegradability of the hydrogels was evaluated by experiments on the base of enzymes and cell culture medium. Poly(aspartic acid) hydrogels possessing only disulfide bridges as cross-links proved to be degradable by collagenase I. The MG-63 cells showed healthy, fibroblast-like morphology on the double cross-linked and RGD modified hydrogels. Thiolated poly(aspartic acid) based hydrogels provide ideal conditions for adhesion, survival, proliferation, and migration of osteoblast-like cells. The highest viability was found on the thiolated PASP gels while the RGD motif had influence on compacted cluster formation of the cells. These biodegradable and biocompatible poly(aspartic acid)-based hydrogels are promising scaffolds for cell cultivation.
authors.affiliation: Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University , Nagyvárad tér 4., H-1089 Budapest, Hungary.
authors.forename: Dávid
authors.initials: D
authors.lastname: Juriga
authors.affiliation: Department of Oral Biology, Semmelweis University , Nagyvárad tér 4., H-1089 Budapest, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Nagy
authors.affiliation: Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University , Nagyvárad tér 4., H-1089 Budapest, Hungary.
authors.forename: Angéla
authors.initials: A
authors.lastname: Jedlovszky-Hajdú
authors.affiliation: Department of Oral Biology, Semmelweis University , Nagyvárad tér 4., H-1089 Budapest, Hungary.|Department in Community Dentistry, Semmelweis University , Üllői út 26., H-1085 Budapest, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Perczel-Kovách
authors.affiliation: State Key Laboratory for Strength and Vibration of Mechanical Structures, International Center for Applied Mechanics and School of Aerospace, Xi'an Jiaotong University , Xi'an 710049, China.
authors.forename: Yong Mei
authors.initials: YM
authors.lastname: Chen
authors.affiliation: Department of Oral Biology, Semmelweis University , Nagyvárad tér 4., H-1089 Budapest, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Varga
authors.affiliation: Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University , Nagyvárad tér 4., H-1089 Budapest, Hungary.|MTA-SE Molecular Biophysics Research Group, Hungarian Academy of Sciences , Széchenyi István tér 9., 1051 Budapest, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Zrínyi
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D049109:Cell Proliferation; D020100:Hydrogels; D010006:Osteoblasts; D012516:Osteosarcoma; D010455:Peptides
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D010455:Peptides; C017645:polyaspartate
keywords: MG-63; RGD; cell viability; poly(aspartic acid) hydrogel; thiolated