title: Role of Nfu1 and Bol3 in iron-sulfur cluster transfer to mitochondrial clients.
abstract: Iron-sulfur (Fe-S) clusters are essential for many cellular processes, ranging from aerobic respiration, metabolite biosynthesis, ribosome assembly and DNA repair. Mutations in NFU1 and BOLA3 have been linked to genetic diseases with defects in mitochondrial Fe-S centers. Through genetic studies in yeast, we demonstrate that Nfu1 functions in a late step of [4Fe-4S] cluster biogenesis that is of heightened importance during oxidative metabolism. Proteomic studies revealed Nfu1 physical interacts with components of the ISA [4Fe-4S] assembly complex and client proteins that need [4Fe-4S] clusters to function. Additional studies focused on the mitochondrial BolA proteins, Bol1 and Bol3 (yeast homolog to human BOLA3), revealing that Bol1 functions earlier in Fe-S biogenesis with the monothiol glutaredoxin, Grx5, and Bol3 functions late with Nfu1. Given these observations, we propose that Nfu1, assisted by Bol3, functions to facilitate Fe-S transfer from the biosynthetic apparatus to the client proteins preventing oxidative damage to [4Fe-4S] clusters.
authors.affiliation: Department of Medicine, University of Utah Health Sciences Center, Salt Lake City, United States.|Department of Biochemistry, University of Utah Health Sciences Center, Salt Lake City, United States.
authors.forename: Andrew
authors.initials: A
authors.lastname: Melber
authors.affiliation: Department of Medicine, University of Utah Health Sciences Center, Salt Lake City, United States.|Department of Biochemistry, University of Utah Health Sciences Center, Salt Lake City, United States.
authors.forename: Un
authors.initials: U
authors.lastname: Na
authors.affiliation: Department of Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, United States.
authors.forename: Ajay
authors.initials: A
authors.lastname: Vashisht
authors.affiliation: Institut für Zytobiologie, Philipps-Universität Marburg, Marburg, Germany.
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Weiler
authors.affiliation: Institut für Zytobiologie, Philipps-Universität Marburg, Marburg, Germany.|LOEWE Zentrum für Synthetische Mikrobiologie SynMikro, Marburg, Germany.
authors.forename: Roland
authors.identifier: 0000-0002-8345-6518
authors.initials: R
authors.lastname: Lill
authors.affiliation: Department of Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, United States.
authors.forename: James A
authors.initials: JA
authors.lastname: Wohlschlegel
authors.affiliation: Department of Medicine, University of Utah Health Sciences Center, Salt Lake City, United States.|Department of Biochemistry, University of Utah Health Sciences Center, Salt Lake City, United States.
authors.forename: Dennis R
authors.identifier: 0000-0003-1160-1189
authors.initials: DR
authors.lastname: Winge
mesh_terms: D001692:Biological Transport; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C000624275:Bol1 protein, S cerevisiae; C000624276:Bol3 protein, S cerevisiae; D007506:Iron-Sulfur Proteins; D024101:Mitochondrial Proteins; C121524:NFU1 protein, S cerevisiae; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins
keywords: S. cerevisiae; biochemistry; cofactor biogenesis; iron-sulfur biogenesis; iron-sulfur clusters; mitochondria; oxidative damage