title: Development of garlic bioactive compounds analytical methodology based on liquid phase microextraction using response surface design. Implications for dual analysis: Cooked and biological fluids samples.
abstract: Organosulphur compounds (OSCs) present in garlic (Allium sativum L.) are responsible of several biological properties. Functional foods researches indicate the importance of quantifying these compounds in food matrices and biological fluids. For this purpose, this paper introduces a novel methodology based on dispersive liquid-liquid microextraction (DLLME) coupled to high performance liquid chromatography with ultraviolet detector (HPLC-UV) for the extraction and determination of organosulphur compounds in different matrices. The target analytes were allicin, (E)- and (Z)-ajoene, 2-vinyl-4H-1,2-dithiin (2-VD), diallyl sulphide (DAS) and diallyl disulphide (DADS). The microextraction technique was optimized using an experimental design, and the analytical performance was evaluated under optimum conditions. The desirability function presented an optimal value for 600μL of chloroform as extraction solvent using acetonitrile as dispersant. The method proved to be reliable, precise and accurate. It was successfully applied to determine OSCs in cooked garlic samples as well as blood plasma and digestive fluids.
authors.affiliation: Laboratorio de Cromatografía para Agroalimentos (LabCrom) - Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina; Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Parque General San Martín, Mendoza, Argentina.
authors.forename: Daniela Andrea
authors.initials: DA
authors.lastname: Ramirez
authors.affiliation: Laboratorio de Cromatografía para Agroalimentos (LabCrom) - Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina; Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina; Instituto de Biología Agrícola de Mendoza (IBAM)-CONICET, Mendoza, Argentina.
authors.forename: Daniela Ana
authors.initials: DA
authors.lastname: Locatelli
authors.affiliation: Laboratorio de Cromatografía para Agroalimentos (LabCrom) - Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina; Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina; Instituto de Biología Agrícola de Mendoza (IBAM)-CONICET, Mendoza, Argentina.
authors.forename: Carolina Andrea
authors.initials: CA
authors.lastname: Torres-Palazzolo
authors.affiliation: Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Parque General San Martín, Mendoza, Argentina; Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales (IANIGLA)-CONICET, Mendoza, Argentina.
authors.forename: Jorgelina Cecilia
authors.initials: JC
authors.lastname: Altamirano
authors.affiliation: Laboratorio de Cromatografía para Agroalimentos (LabCrom) - Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina; Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina; Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Parque General San Martín, Mendoza, Argentina; Instituto de Biología Agrícola de Mendoza (IBAM)-CONICET, Mendoza, Argentina. Electronic address: acamargo@fca.uncu.edu.ar.
authors.forename: Alejandra Beatriz
authors.initials: AB
authors.lastname: Camargo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005737:Garlic / Q000737:chemistry*; D059627:Liquid Phase Microextraction / Q000379:methods*
keywords: Biological fluids; DLLME; Garlic; HPLC; Organosulphur compounds