title: Classical swine fever virus replicated poorly in cells from MxA transgenic pigs.
abstract: BACKGROUND
  In addition to their value as livestock, pigs are susceptible to classical swine fever virus (CSFV) and can serve as reservoirs for CSFV, allowing it to develop into an epizootic. CSFV, a pestivirus of the Flaviviridae family, has a single-stranded RNA genome. Recent research has indicated that the human MxA protein inhibits the life cycles of certain RNA viruses, such as members of the Bunyaviridae family, the Flaviviridae family and others.
  
  
  RESULTS
  To produce pigs with antiviral protection against CSFV, transgenic pigs expressing human MxA were generated by nuclear transplantation. Cells from three MxA transgenic piglets were used to investigate in vitro antiviral activity of MxA aganist CSFV, and the results of in vitro indirect immunofluorescence assays, virus titration and real-time PCR indicated that the MxA transgenic pig has an antiviral capacity against CSFV.
  
  
  CONCLUSIONS
  Transgene with human MxA on pigs is feasible. High levels of MxA expression do inhibit CSFV in vitro at early time points post-infection at 60-96dpi.
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, China. yichengzhao@live.cn.|College of Animal Sciences, Jilin University, Changchun, China. yichengzhao@live.cn.
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, China.
authors.forename: Tiedong
authors.initials: T
authors.lastname: Wang
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yao
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, China.|College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, China.
authors.forename: Chunbo
authors.initials: C
authors.lastname: Teng
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, China. oyhs64@hotmail.com.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002478:Cells, Cultured; D006692:Classical Swine Fever Virus / Q000502:physiology*; D007668:Kidney / Q000166:cytology; D008297:Male; D064547:Myxovirus Resistance Proteins / Q000235:genetics / Q000378:metabolism*; D053652:Nuclear Transfer Techniques; D013552:Swine; D013623:Tail / Q000166:cytology; D014470:Umbilical Cord / Q000166:cytology; D014779:Virus Replication / Q000502:physiology*
chemical_list: C579071:MX1 protein, human; D064547:Myxovirus Resistance Proteins
keywords: Classical swine fever virus; MxA; Nuclear transplantation; Transgenic pig