title: Spatial Distribution of Eukaryotic Communities Using High-Throughput Sequencing Along a Pollution Gradient in the Arsenic-Rich Creek Sediments of Carnoulès Mine, France.
abstract: Microscopic eukaryotes play a key role in ecosystem functioning, but their diversity remains largely unexplored in most environments. To advance our knowledge of eukaryotic microorganisms and the factors that structure their communities, high-throughput sequencing was used to characterize their diversity and spatial distribution along the pollution gradient of the acid mine drainage at Carnoulès (France). A total of 16,510 reads were retrieved leading to the identification of 323 OTUs after normalization. Phylogenetic analysis revealed a quite diverse eukaryotic community characterized by a total of eight high-level lineages including 37 classes. The majority of sequences were clustered in four main groups: Fungi, Stramenopiles, Alveolata and Viridiplantae. The Reigous sediments formed a succession of distinct ecosystems hosting contrasted eukaryotic communities whose structure appeared to be at least partially correlated with sediment mineralogy. The concentration of arsenic in the sediment was shown to be a significant factor driving the eukaryotic community structure along this continuum.
authors.affiliation: Laboratoire HydroSciences Montpellier, UMR 5569, Université de Montpellier, CC0057 (MSE), 163 rue Auguste Broussonet, 34090, Montpellier, France. aurelie.volant@univ-montp2.fr.
authors.forename: A
authors.initials: A
authors.lastname: Volant
authors.affiliation: Laboratoire HydroSciences Montpellier, UMR 5569, Université de Montpellier, CC0057 (MSE), 163 rue Auguste Broussonet, 34090, Montpellier, France.
authors.forename: M
authors.initials: M
authors.lastname: Héry
authors.affiliation: Laboratoire HydroSciences Montpellier, UMR 5569, Université de Montpellier, CC0057 (MSE), 163 rue Auguste Broussonet, 34090, Montpellier, France.
authors.forename: A
authors.initials: A
authors.lastname: Desoeuvre
authors.affiliation: Laboratoire HydroSciences Montpellier, UMR 5569, Université de Montpellier, CC0057 (MSE), 163 rue Auguste Broussonet, 34090, Montpellier, France.
authors.forename: C
authors.initials: C
authors.lastname: Casiot
authors.affiliation: Institut de Minéralogie et de Physique des Milieux Condensés, IMPMC, UMR 7590 (CNRS, Université Pierre et Marie Curie/Paris 6), 4 place Jussieu, 75252, Paris, France.
authors.forename: G
authors.initials: G
authors.lastname: Morin
authors.affiliation: Laboratoire de Génétique Moléculaire, Génomique, Microbiologie, GMGM, UMR 7156 (Université de Strasbourg, CNRS), Département Microorganismes, Génomes, Environnement, 28 Rue Goethe, 67083, Strasbourg, France.
authors.forename: P N
authors.initials: PN
authors.lastname: Bertin
authors.affiliation: Laboratoire HydroSciences Montpellier, UMR 5569, Université de Montpellier, CC0057 (MSE), 163 rue Auguste Broussonet, 34090, Montpellier, France.
authors.forename: O
authors.initials: O
authors.lastname: Bruneel
mesh_terms: D056893:Alveolata / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001151:Arsenic / Q000032:analysis*; D001483:Base Sequence; D044822:Biodiversity; D002965:Classification; D004247:DNA; D004463:Ecology; D017753:Ecosystem; D004787:Environmental Pollution / Q000032:analysis*; D056890:Eukaryota / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification*; D005602:France; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000737:chemistry*; D057166:High-Throughput Screening Assays; D006863:Hydrogen-Ion Concentration; D008906:Mining; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D045483:Rivers; D017421:Sequence Analysis; D058009:Stramenopiles / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D057948:Viridiplantae / Q000145:classification / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000032:analysis; D014876:Water Pollution
chemical_list: D012337:RNA, Ribosomal, 18S; D014874:Water Pollutants, Chemical; D004247:DNA; D001151:Arsenic
keywords: Acid mine drainage; Arsenic; Community spatial dynamics; Eukaryotic diversity