title: Discovery of clubroot-resistant genes in Brassica napus by transcriptome sequencing.
abstract: Clubroot significantly affects plants of the Brassicaceae family and is one of the main diseases causing serious losses in B. napus yield. Few studies have investigated the clubroot-resistance mechanism in B. napus. Identification of clubroot-resistant genes may be used in clubroot-resistant breeding, as well as to elucidate the molecular mechanism behind B. napus clubroot-resistance. We used three B. napus transcriptome samples to construct a transcriptome sequencing library by using Illumina HiSeq™ 2000 sequencing and bioinformatic analysis. In total, 171 million high-quality reads were obtained, containing 96,149 unigenes of N50-value. We aligned the obtained unigenes with the Nr, Swiss-Prot, clusters of orthologous groups, and gene ontology databases and annotated their functions. In the Kyoto encyclopedia of genes and genomes database, 25,033 unigenes (26.04%) were assigned to 124 pathways. Many genes, including broad-spectrum disease-resistance genes, specific clubroot-resistant genes, and genes related to indole-3-acetic acid (IAA) signal transduction, cytokinin synthesis, and myrosinase synthesis in the Huashuang 3 variety of B. napus were found to be related to clubroot-resistance. The effective clubroot-resistance observed in this variety may be due to the induced increased expression of these disease-resistant genes and strong inhibition of the IAA signal transduction, cytokinin synthesis, and myrosinase synthesis. The homology observed between unigenes 0048482, 0061770 and the Crr1 gene shared 94% nucleotide similarity. Furthermore, unigene 0061770 could have originated from an inversion of the Crr1 5'-end sequence.
authors.affiliation: College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
authors.forename: S W
authors.initials: SW
authors.lastname: Chen
authors.affiliation: College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
authors.forename: T
authors.initials: T
authors.lastname: Liu
authors.affiliation: College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Tropical Crops, Yunnan Agricultural University, Kunming, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Agricultural Experimental Teaching Center, Yunnan Agricultural University, Kunming, China.
authors.forename: S D
authors.initials: SD
authors.lastname: Peng
authors.affiliation: College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
authors.forename: M B
authors.initials: MB
authors.lastname: Bai
authors.affiliation: College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Li
authors.affiliation: College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhou
authors.affiliation: College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China linliangbin-63@163.com.
authors.forename: L B
authors.initials: LB
authors.lastname: Lin
mesh_terms: D001483:Base Sequence; D029688:Brassica napus / Q000235:genetics* / Q000469:parasitology; D060467:Disease Resistance / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D006790:Host-Parasite Interactions; D053858:Metabolic Networks and Pathways; D058977:Molecular Sequence Annotation; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology; D018517:Plant Roots / Q000235:genetics / Q000469:parasitology; D056909:Plasmodiophorida / Q000502:physiology; D017423:Sequence Analysis, RNA; D059467:Transcriptome