title: miR-297 acts as an oncogene by targeting GPC5 in lung adenocarcinoma.
abstract: OBJECTIVE
  Emerging studies have demonstrated that microRNAs (miRNAs) play crucial roles in carcinogenesis of many developing human tumours. However, the functions and mechanisms of miR-297 in lung cancer have, up to now, been largely undefined.
  
  
  METHODS
  Here, miR-297 expression was measured in lung adenocarcinoma tissues and cell lines, using qRT-PCR. Lung adenocarcinoma cell line was treated with an miR-297 mimic. MTT and colony analysis were performed to detect cell proliferation and colony formation. The direct target gene of miR-297 was assessed by qRT-PCR, Western blotting and luciferase assays.
  
  
  RESULTS
  We demonstrated that miR-297 expression was upregulated in lung adenocarcinomas compared to adjacent normal tissues. Expression of miR-297 was also upregulated in tested lung adenocarcinoma cell lines. Ectopic expression of miR-297 enhanced lung adenocarcinoma cell proliferation and colony formation. Furthermore, overexpression of miR-297 promoted cell migration and invasion. In addition, we identified Glypican-5 (GPC5) as a direct target gene of miR-297 in lung adenocarcinoma cells. Expression of GPC5 was downregulated in both lung adenocarcinoma tissues and cell lines. Moreover, expression of GPC5 was inversely associated with expression of miR-297 in lung adenocarcinoma tissues.
  
  
  CONCLUSIONS
  These results suggest that miR-297 acted as an oncogenic miRNA, partly by targeting GPC5, adenocarcinoma of the lung.
authors.affiliation: Department of Radiation and Chemotherapy, Hebei Province Cangzhou Hospital of Integrated Traditional and Western Medicine, Cangzhou, Hebei, 061000, China.
authors.forename: Yunchuan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Radiation and Chemotherapy, Hebei Province Cangzhou Hospital of Integrated Traditional and Western Medicine, Cangzhou, Hebei, 061000, China.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Radiation and Chemotherapy, Hebei Province Cangzhou Hospital of Integrated Traditional and Western Medicine, Cangzhou, Hebei, 061000, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Radiation and Chemotherapy, Hebei Province Cangzhou Hospital of Integrated Traditional and Western Medicine, Cangzhou, Hebei, 061000, China.
authors.forename: Xiangkun
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Radiation and Chemotherapy, Hebei Province Cangzhou Hospital of Integrated Traditional and Western Medicine, Cangzhou, Hebei, 061000, China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Radiation and Chemotherapy, Hebei Province Cangzhou Hospital of Integrated Traditional and Western Medicine, Cangzhou, Hebei, 061000, China. jianqiangbi@tom.com.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Bi
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D053673:Glypicans / Q000235:genetics*; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009857:Oncogenes; D015854:Up-Regulation
chemical_list: C106228:GPC5 protein, human; D053673:Glypicans; C000613758:MIRN297 microRNA, human; D035683:MicroRNAs