title: Serum Antibody Profile during Colonization of the Mouse Gut by Candida albicans: Relevance for Protection during Systemic Infection.
abstract: Candida albicans is a commensal microorganism in the oral cavity and gastrointestinal and urogenital tracts of most individuals that acts as an opportunistic pathogen when the host immune response is reduced. Here, we established different immunocompetent murine models to analyze the antibody responses to the C. albicans proteome during commensalism, commensalism followed by infection, and infection (C, C+I, and I models, respectively). Serum anti-C. albicans IgG antibody levels were higher in colonized mice than in infected mice. The antibody responses during gut commensalism (up to 55 days of colonization) mainly focused on C. albicans proteins involved in stress response and metabolism and differed in both models of commensalism. Different serum IgG antibody-reactivity profiles were also found over time among the three murine models. C. albicans gut colonization protected mice from an intravenous lethal fungal challenge, emphasizing the benefits of fungal gut colonization. This work highlights the importance of fungal gut colonization for future immune prophylactic therapies.
authors.affiliation: Department of Microbiology II, Faculty of Pharmacy, Complutense University of Madrid and Ramón y Cajal Institute of Health Research (IRYCIS) , Plaza Ramón y Cajal s/n, 28040 Madrid, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: Huertas
authors.affiliation: Department of Microbiology II, Faculty of Pharmacy, Complutense University of Madrid and Ramón y Cajal Institute of Health Research (IRYCIS) , Plaza Ramón y Cajal s/n, 28040 Madrid, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Prieto
authors.affiliation: Department of Microbiology II, Faculty of Pharmacy, Complutense University of Madrid and Ramón y Cajal Institute of Health Research (IRYCIS) , Plaza Ramón y Cajal s/n, 28040 Madrid, Spain.
authors.forename: Aida
authors.initials: A
authors.lastname: Pitarch
authors.affiliation: Department of Microbiology II, Faculty of Pharmacy, Complutense University of Madrid and Ramón y Cajal Institute of Health Research (IRYCIS) , Plaza Ramón y Cajal s/n, 28040 Madrid, Spain.
authors.forename: Concha
authors.initials: C
authors.lastname: Gil
authors.affiliation: Department of Microbiology II, Faculty of Pharmacy, Complutense University of Madrid and Ramón y Cajal Institute of Health Research (IRYCIS) , Plaza Ramón y Cajal s/n, 28040 Madrid, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Pla
authors.affiliation: Department of Microbiology II, Faculty of Pharmacy, Complutense University of Madrid and Ramón y Cajal Institute of Health Research (IRYCIS) , Plaza Ramón y Cajal s/n, 28040 Madrid, Spain.
authors.forename: Rosalía
authors.initials: R
authors.lastname: Díez-Orejas
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D002176:Candida albicans / Q000254:growth & development / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology* / Q000382:microbiology / Q000401:mortality; D005260:Female; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology*; D000069196:Gastrointestinal Microbiome / Q000276:immunology; D041981:Gastrointestinal Tract / Q000276:immunology / Q000382:microbiology; D054884:Host-Pathogen Interactions; D007074:Immunoglobulin G / Q000097:blood*; D051379:Mice; D008810:Mice, Inbred C57BL; D016019:Survival Analysis; D013559:Symbiosis / Q000276:immunology
chemical_list: D000908:Antibodies, Fungal; D005656:Fungal Proteins; D007074:Immunoglobulin G
keywords: Candida albicans; IgG antibody-reactivity profile; commensalism; fungal gut colonization