title: Native Prussian carp (Carassius gibelio) health status, biochemical and histological responses to treated wastewaters.
abstract: The aim of this study was to assess the impact of treated wastewaters on native wild Prussian carp inhabiting effluent-receiving waters (ERC) receiving municipal and sugar plant treated wastewaters, further downstream waters (DW), and a detached canal unaffected by the WWTP activities. To that end, general fish health status was determined, including plasma biochemical, haematological, oxidative stress and tissue histopathological indices, over three seasons. The greatest tissue alterations were in fall in ERC during sugar beet processing, as hypertrophy of gill epithelial and interlamellar cells, necrosis and lymphocytic infiltration, hyperplasia and hypertrophy of renal tubules, distention of hepatic sinusoids. In fall the lowest leukocytes, lymphocytes and granulocytes (2467 ± 565, 1333 ± 264, 1133 ± 488 cells/μL respectively), as well as highest plasma ALP (52.7 ± 19.39 U/L) were measured. ERC in fall had the highest ammonium (20 mg/L), nitrite (1.48 mg/L), nitrate (13.4 mg/L), and lowest dissolved O2(1.23 mg/L). Gill, kidney and liver alterations, and the highest plasma cholesterol (9.1 ± 1.98 mmol/L) were noted in DW fish in fall. Tissue morphology during sugar cane processing seems a consequence of cellular and structural tissue integrity loss. Structural heterogeneity of gills and spleen was enhanced with increasing concentrations of heavy metals and correlated with oxidative stress (SOD 392.5 ± 77.28 U/L). Monogenean infestation was moderate in ERC fish in all seasons compared with DW fish. Prussian carp biological responses to multiple stressors, measured by the effects of WWTP on blood and tissue parameters, reached far downstream and were not of localized nature. This study demonstrated that in aquatic environments impacted with complex contaminants acting synergistically, causal relationships between biological responses and environmental stressors should be interpreted. Integrated histopathological, haematological and biochemical findings are valuable biomarkers for native fish adaptive patterns and monitoring of water quality/pollution of freshwater ecosystems.
authors.affiliation: Laboratory for Biotechnology in Aquaculture, Ruđer Bošković Institute, Bijenička 54, Zagreb, Croatia. Electronic address: ntopic@irb.hr.
authors.forename: Natalija
authors.initials: N
authors.lastname: Topić Popović
authors.affiliation: Laboratory for Biotechnology in Aquaculture, Ruđer Bošković Institute, Bijenička 54, Zagreb, Croatia.
authors.forename: Ivančica
authors.initials: I
authors.lastname: Strunjak-Perović
authors.affiliation: Laboratory for Biotechnology in Aquaculture, Ruđer Bošković Institute, Bijenička 54, Zagreb, Croatia.
authors.forename: Josip
authors.initials: J
authors.lastname: Barišić
authors.affiliation: Virkom d.o.o, Public Water Supply and Wastewater Services, Kralja Petra Krešimira IV 30, Virovitica, Croatia.
authors.forename: Slavko
authors.initials: S
authors.lastname: Kepec
authors.affiliation: Laboratory for Biotechnology in Aquaculture, Ruđer Bošković Institute, Bijenička 54, Zagreb, Croatia.
authors.forename: Margita
authors.initials: M
authors.lastname: Jadan
authors.affiliation: Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia.
authors.forename: Blanka
authors.initials: B
authors.lastname: Beer-Ljubić
authors.affiliation: Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Matijatko
authors.affiliation: Chair for Fish Diseases and Fisheries Biology, Faculty of Veterinary Medicine, Ludwig-Maximilians University Munich, Germany.
authors.forename: Dušan
authors.initials: D
authors.lastname: Palić
authors.affiliation: Division of Zoology, Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, Zagreb, Croatia.
authors.forename: Goran
authors.initials: G
authors.lastname: Klobučar
authors.affiliation: Laboratory for Biotechnology in Aquaculture, Ruđer Bošković Institute, Bijenička 54, Zagreb, Croatia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Babić
authors.affiliation: Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, Zagreb, Croatia.
authors.forename: Jasenka
authors.initials: J
authors.lastname: Gajdoš Kljusurić
authors.affiliation: Laboratory for Biotechnology in Aquaculture, Ruđer Bošković Institute, Bijenička 54, Zagreb, Croatia.
authors.forename: Rozelindra
authors.initials: R
authors.lastname: Čož-Rakovac
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000502:physiology*; D005618:Fresh Water; D005880:Gills / Q000187:drug effects; D006304:Health Status; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D019216:Metals, Heavy / Q000737:chemistry / Q000633:toxicity*; D018384:Oxidative Stress; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*; D018508:Water Purification
chemical_list: D019216:Metals, Heavy; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Effluent; Histopathology; Plasma biochemistry; Prussian carp