title: Oncogenic Transformation of Human-Derived Gastric Organoids.
abstract: The culture of organoids has represented a significant advancement in the gastrointestinal research field. Previous research studies have described the oncogenic transformation of human intestinal and mouse gastric organoids. Here we detail the protocol for the oncogenic transformation and orthotopic transplantation of human-derived gastric organoids.
authors.affiliation: Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
authors.forename: Nina
authors.initials: N
authors.lastname: Bertaux-Skeirik
authors.affiliation: Biology Department, University of Puerto Rico-Río Piedras, San Juan, Puerto Rico.
authors.forename: Jomaris
authors.initials: J
authors.lastname: Centeno
authors.affiliation: Department of Pediatrics, W.F. Maternal & Child Health Hospital, 76 Qingnian Road, Weifang, 261011, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Internal Medicine, University of Cincinnati, Cincinnati, OH, USA.
authors.forename: Joel
authors.initials: J
authors.lastname: Gabre
authors.affiliation: Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert B. Sabin Way, Room 4255 MSB, Cincinnati, OH, 45267-0576, USA. yana.zavros@uc.edu.
authors.forename: Yana
authors.initials: Y
authors.lastname: Zavros
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D064593:Heterografts; D006801:Humans; D051379:Mice; D009940:Organoids / Q000473:pathology* / Q000637:transplantation*; D013274:Stomach Neoplasms / Q000473:pathology*
keywords: CRISPR/Cas-9; Human fundic gastric organoids; Nucleofection; Organoid orthotopic transplantation