title: Image Analysis of DNA Fiber and Nucleus in Plants.
abstract: Advances in cytology have led to the application of a wide range of visualization methods in plant genome studies. Image analysis methods are indispensable tools where morphology, density, and color play important roles in the biological systems. Visualization and image analysis methods are useful techniques in the analyses of the detailed structure and function of extended DNA fibers (EDFs) and interphase nuclei. The EDF is the highest in the spatial resolving power to reveal genome structure and it can be used for physical mapping, especially for closely located genes and tandemly repeated sequences. One the other hand, analyzing nuclear DNA and proteins would reveal nuclear structure and functions. In this chapter, we describe the image analysis protocol for quantitatively analyzing different types of plant genome, EDFs and interphase nuclei.
authors.affiliation: Graduate School of Human Development and Environment, Kobe University, Kobe, 657-8501, Hyogo, Japan.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Ohmido
authors.affiliation: Advanced Analysis Center, National Agriculture and Food Research Organization, Tsukuba, 305-8602, Ibaraki, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Wako
authors.affiliation: Yamanashi Prefectural Agritechnology Center, 1100, Shimoimai, 400-0105, Kai, Yamanashi, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Kato
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871, Osaka, Japan. kfukui@bio.eng.osaka-u.ac.jp.
authors.forename: Kiichi
authors.initials: K
authors.lastname: Fukui
mesh_terms: D002467:Cell Nucleus; D018744:DNA, Plant / Q000032:analysis* / Q000737:chemistry; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007399:Interphase; D059828:Plant Cells; D012984:Software
chemical_list: D018744:DNA, Plant
keywords: CHIAS-interphase; CHIAS-straight; Extended DNA fibers (EDFs); Image analysis; Isolated nuclei