title: Identification of Novel Genomic Islands in Liverpool Epidemic Strain of Pseudomonas aeruginosa Using Segmentation and Clustering.
abstract: Pseudomonas aeruginosa is an opportunistic pathogen implicated in a myriad of infections and a leading pathogen responsible for mortality in patients with cystic fibrosis (CF). Horizontal transfers of genes among the microorganisms living within CF patients have led to highly virulent and multi-drug resistant strains such as the Liverpool epidemic strain of P. aeruginosa, namely the LESB58 strain that has the propensity to acquire virulence and antibiotic resistance genes. Often these genes are acquired in large clusters, referred to as "genomic islands (GIs)." To decipher GIs and understand their contributions to the evolution of virulence and antibiotic resistance in P. aeruginosa LESB58, we utilized a recursive segmentation and clustering procedure, presented here as a genome-mining tool, "GEMINI." GEMINI was validated on experimentally verified islands in the LESB58 strain before examining its potential to decipher novel islands. Of the 6062 genes in P. aeruginosa LESB58, 596 genes were identified to be resident on 20 GIs of which 12 have not been previously reported. Comparative genomics provided evidence in support of our novel predictions. Furthermore, GEMINI unraveled the mosaic structure of islands that are composed of segments of likely different evolutionary origins, and demonstrated its ability to identify potential strain biomarkers. These newly found islands likely have contributed to the hyper-virulence and multidrug resistance of the Liverpool epidemic strain of P. aeruginosa.
authors.affiliation: Department of Biological Sciences, University of North Texas Denton, TX, USA.
authors.forename: Mehul
authors.initials: M
authors.lastname: Jani
authors.affiliation: Department of Human and Molecular Genetics, Herbert Wertheim College of Medicine Global Health Consortium, and Biomolecular Sciences Institute, Florida International University Miami, FL, USA.
authors.forename: Kalai
authors.initials: K
authors.lastname: Mathee
authors.affiliation: Department of Biological Sciences, University of North TexasDenton, TX, USA; Department of Mathematics, University of North TexasDenton, TX, USA.
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Azad
keywords: Liverpool epidemic strain; Pseudomonas aeruginosa; antibiotic resistance; cystic fibrosis; genome segmentation; genomic islands; pathogen evolution; virulence