title: Serum protein profiling in diffuse large B-cell lymphoma.
abstract: OBJECTIVE
  The aim of this pilot study was to conduct a nontargeted exploratory proteomics profiling analysis on sera obtained from patients diagnosed with diffuse large B-cell lymphoma (DLBCL) with the goal of identifying disease-specific biomarkers.
  
  
  METHODS
  Sera from 87 participants (57 chemotherapy-naïve diffuse DLBCL patients, 30 controls frequency-matched by age group and World Health Organization (WHO) BMI categories) that were part of a large San Francisco Bay Area case-control study of non-Hodgkin lymphoma were analyzed by liquid chromatography combined with tandem mass spectrometry.
  
  
  RESULTS
  Thirty-five proteins (p-adjusted <0.05) were identified as differentially abundant between the DLBCL patients at various disease stages as compared to the controls. Of these, five proteins were randomly selected for further confirmation by ELISA: adiponectin (AdipoQ), cluster of differentiation 14 (CD14), heparin sulfate proteoglycan core protein (HSPG2), extracellular matrix 1 (ECM1), and alpha-1-antichymotrypsin (ACT). These proteins were statistically significantly elevated by 68.8, 37.0, 61.6, 68.0, and 32.0%, respectively, in DLBCL patient sera as compared to controls.
  
  
  CONCLUSIONS
  These preliminary data when combined with other cancer-related data regarding these proteins warrant continued research in clinical and large prospective studies to clarify the role for these biomarkers in DLBCL pathogenesis and/or prognosis.
authors.affiliation: Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, USA.|Department of Epidemiology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: Jacques
authors.initials: J
authors.lastname: Riby
authors.affiliation: Department of Epidemiology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA.|Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: James
authors.initials: J
authors.lastname: Mobley
authors.affiliation: Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, USA.|Department of Epidemiology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology and Biostatistics, University of California at San Francisco, San Francisco, CA, USA.
authors.forename: Paige M
authors.initials: PM
authors.lastname: Bracci
authors.affiliation: Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, USA.|Department of Epidemiology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: Christine F
authors.initials: CF
authors.lastname: Skibola
mesh_terms: D052242:Adiponectin / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D001798:Blood Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D016403:Lymphoma, Large B-Cell, Diffuse / Q000097:blood / Q000473:pathology*; D013058:Mass Spectrometry; D008875:Middle Aged; D010455:Peptides / Q000032:analysis; D010865:Pilot Projects
chemical_list: D052242:Adiponectin; D001798:Blood Proteins; C490607:ECM1 protein, human; D016326:Extracellular Matrix Proteins; D019812:Heparan Sulfate Proteoglycans; D018950:Lipopolysaccharide Receptors; D010455:Peptides; C071927:perlecan
keywords: Biomarker; DLBCL; LCMS; NHL; Serum proteomics