title: Cloning and characterization of the UBC gene from lotus (Nelumbo nucifera).
abstract: Protein ubiquitination is extensively involved in the regulation of a considerable number of physiological processes in plant cells. E2 (ubiquitin-conjugating enzyme, UBC), one of the essential enzymes of eukaryotic ubiquitination, catalyzes protein ubiquitination together with E1 and E3. In this study, we cloned four full-length cDNA NnUBCs of Nelumbo nucifera. With the same coding sequence length of 459 bp and coding 153 amino acids, these four genes are highly homologous with the AtUBC1 and AtUBC2 of Arabidopsis thaliana. Quantitative fluorescence polymerase chain reaction showed that these four genes exhibited different expression patterns in different tissues of N. nucifera. Overall, the expression of NnUBC3 was the highest in all plant tissues. Tests of different stress treatments showed that NnUBC3 plays an important role in response to heat, salt, and drought stresses in N. nucifera. Moreover, transgenic Arabidopsis plants (Atubc1-1Atubc2-1 mutant) expressing NnUBC3 presented a wild-type phenotype, indicating that NnUBC3 performs the same function as AtUBC1 and AtUBC2.
authors.affiliation: College of Forestry and Life Sciences, Chongqing University of Arts and Sciences, Chongqing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Diao
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: G L
authors.initials: GL
authors.lastname: Li
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: A Q
authors.initials: AQ
authors.lastname: Yu
authors.affiliation: White Lotus Research Institute of Guangchang, Guangchang, Jiangxi Province, China.
authors.forename: X W
authors.initials: XW
authors.lastname: Zheng
authors.affiliation: White Lotus Research Institute of Guangchang, Guangchang, Jiangxi Province, China.
authors.forename: K Q
authors.initials: KQ
authors.lastname: Xie
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Y W
authors.initials: YW
authors.lastname: Wang
authors.affiliation: Lotus Engineering Research Center of Hubei Province, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: M Q
authors.initials: MQ
authors.lastname: Zhou
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China mingjun@caas.cn.
authors.forename: J
authors.initials: J
authors.lastname: Ming
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D031653:Nelumbo / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D044763:Ubiquitin-Conjugating Enzymes