title: In vivo evaluation of adipose- and muscle-derived stem cells as a treatment for nonhealing diabetic wounds using multimodal microscopy.
abstract: Impaired skin wound healing is a significant comorbid condition of diabetes, which often results in nonhealing diabetic ulcers due to poor peripheral microcirculation, among other factors. The effectiveness of the regeneration of adipose-derived stem cells (ADSCs) and muscle-derived stem cells (MDSCs) was assessed using an integrated multimodal microscopy system equipped with two-photon fluorescence and second-harmonic generation imaging. These imaging modalities, integrated in a single platform for spatial and temporal coregistration, allowed us to monitor in vivo changes in the collagen network and cell dynamics in a skin wound. Fluorescently labeled ADSCs and MDSCs were applied topically to the wound bed of wild-type and diabetic (db/db) mice following punch biopsy. Longitudinal imaging demonstrated that ADSCs and MDSCs provided remarkable capacity for improved diabetic wound healing, and integrated microscopy revealed a more organized collagen remodeling in the wound bed of treated mice. The results from this study verify the regenerative capacity of stem cells toward healing and, with multimodal microscopy, provide insight regarding their impact on the skin microenvironment. The optical method outlined in this study, which has the potential for in vivo human use, may optimize the care and treatment of diabetic nonhealing wounds.
authors.affiliation: University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United StatesbUniversity of Illinois at Urbana-Champaign, Department of Bioengineering, 1270 Digital Compu.
authors.forename: Joanne
authors.initials: J
authors.lastname: Li
authors.affiliation: University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United StatescUniversity of Illinois at Urbana-Champaign, Department of Kinesiology and Community Health.
authors.forename: Yair
authors.initials: Y
authors.lastname: Pincu
authors.affiliation: University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United StatesbUniversity of Illinois at Urbana-Champaign, Department of Bioengineering, 1270 Digital Compu.
authors.forename: Marina
authors.initials: M
authors.lastname: Marjanovic
authors.affiliation: University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United StatesdUniversity of Illinois at Urbana-Champaign, Department of Electrical and Computer Engineerin.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Bower
authors.affiliation: University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United States.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Chaney
authors.affiliation: University of Illinois at Urbana-Champaign, Illinois Health Sciences Initiative, 611 West Park Street, Urbana, Illinois 61801, United States.
authors.forename: Tor
authors.initials: T
authors.lastname: Jensen
authors.affiliation: University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United StatescUniversity of Illinois at Urbana-Champaign, Department of Kinesiology and Community Health.
authors.forename: Marni D
authors.initials: MD
authors.lastname: Boppart
authors.affiliation: University of Illinois at Urbana-Champaign, Beckman Institute for Advanced Science and Technology, 405 North Mathews Avenue, Urbana, Illinois 61801, United StatesbUniversity of Illinois at Urbana-Champaign, Department of Bioengineering, 1270 Digital Compu.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Boppart
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D048909:Diabetes Complications / Q000628:therapy*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D064847:Multimodal Imaging; D009132:Muscles / Q000166:cytology*; D012867:Skin / Q000000981:diagnostic imaging*; D033581:Stem Cell Transplantation; D014945:Wound Healing; D014947:Wounds and Injuries / Q000628:therapy*