title: A ratiometric fluorescent sensor for the mitochondrial copper pool.
abstract: Copper plays a key role in the modulation of cellular function, defence, and growth. Here we present InCCu1, a ratiometric fluorescent sensor for mitochondrial copper, which changes from red to blue emission in the presence of Cu(i). Employing this probe in microscopy and flow cytometry, we show that cisplatin-treated cells have an impaired ability to accumulate copper in the mitochondria.
authors.affiliation: School of Chemistry, The University of Sydney, NSW 2006, Australia. Elizabeth.new@sydney.edu.au.
authors.forename: Clara
authors.initials: C
authors.lastname: Shen
authors.forename: Jacek L
authors.initials: JL
authors.lastname: Kolanowski
authors.forename: Carmen M-N
authors.initials: CM
authors.lastname: Tran
authors.forename: Amandeep
authors.initials: A
authors.lastname: Kaur
authors.forename: Mia C
authors.initials: MC
authors.lastname: Akerfeldt
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Rahme
authors.forename: Trevor W
authors.initials: TW
authors.lastname: Hambley
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: New
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D015374:Biosensing Techniques / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D003300:Copper / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D003300:Copper; D002945:Cisplatin