title: Piscinibacter defluvii sp. nov., isolated from a sewage treatment plant, and emended description of the genus Piscinibacter Stackebrandt et al. 2009.
abstract: A Gram-staining-negative, strictly aerobic bacterium, designated SH-1T, was isolated from activated sludge in Korea. Cells were motile rods with a single polar flagellum, showing oxidase-positive and catalase-negative activities. Growth was observed at 25-40 °C (optimum, 37 °C), pH 6.0-9.5 (optimum, pH 7.0) and with 0-0.5 % (w/v) NaCl (optimum, 0 %). Strain SH-1T contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0, C12 : 0, summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c) and C10 : 0 3-OH as the major fatty acids and ubiquinone-8 as the sole isoprenoid quinone. Phosphatidylethanolamine was the major polar lipid, and diphosphatidylglycerol, phosphatidylglycerol, one aminophospholipid, one phospholipid, five unidentified aminolipids and two unidentified lipids were also detected as the minor polar lipids. The predominant polyamines were 2-hydroxyputrescine, cadaverine and putrescine. The DNA G+C content was 69.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SH-1T formed a tight phyletic lineage with Piscinibacter aquaticus IMCC1728T with a 98.3 % sequence similarity. However, the DNA-DNA relatedness value between strain SH-1T and the type strain of P. aquaticus was 38.0±1.8 %. On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that strain SH-1T represents a novel species of the genus Piscinibacter, for which the name Piscinibacter defluvii sp. nov. is proposed. The type strain is SH-1T (=KACC 18594T=JCM 31230T). An emended description of the genus Piscinibacter is also proposed.
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D042521:Burkholderiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011700:Putrescine / Q000031:analogs & derivatives / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014451:Ubiquinone; C002952:2-hydroxyputrescine; C030778:ubiquinone 8; D011700:Putrescine