title: Cholesterol-dependent Conformational Plasticity in GPCR Dimers.
abstract: The organization and function of the serotonin1A receptor, an important member of the GPCR family, have been shown to be cholesterol-dependent, although the molecular mechanism is not clear. We performed a comprehensive structural and dynamic analysis of dimerization of the serotonin1A receptor by coarse-grain molecular dynamics simulations totaling 3.6 ms to explore the molecular details of its cholesterol-dependent association. A major finding is that the plasticity and flexibility of the receptor dimers increase with increased cholesterol concentration. In particular, a dimer interface formed by transmembrane helices I-I was found to be sensitive to cholesterol. The modulation of dimer interface appears to arise from a combination of direct cholesterol occupancy and indirect membrane effects. Interestingly, the presence of cholesterol at the dimer interface is correlated with increased dimer plasticity and flexibility. These results represent an important step in characterizing the molecular interactions in GPCR organization with potential relevance to therapeutic interventions.
authors.affiliation: CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411 008, India.
authors.forename: Xavier
authors.initials: X
authors.lastname: Prasanna
authors.affiliation: CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411 008, India.
authors.forename: Durba
authors.initials: D
authors.lastname: Sengupta
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
authors.forename: Amitabha
authors.initials: A
authors.lastname: Chattopadhyay