title: Roostocks/Scion/Nitrogen Interactions Affect Secondary Metabolism in the Grape Berry.
abstract: The present work investigates the interactions between soil content, rootstock, and scion by focusing on the effects of roostocks and nitrogen supply on grape berry content. Scions of Cabernet Sauvignon (CS) and Pinot Noir (PN) varieties were grafted either on Riparia Gloire de Montpellier (RGM) or 110 Richter (110R) rootstock. The 4 rooststock/scion combinations were fertilized with 3 different levels of nitrogen after fruit set. Both in 2013 and 2014, N supply increased N uptake by the plants, and N content both in vegetative and reproductory organs. Rootstock, variety and year affected berry weight at harvest, while nitrogen did not affect significantly this parameter. Grafting on RGM consistently increased berry weight compared to 110R. PN consistently produced bigger berries than CS. CS berries were heavier in 2014 than in 2013, but the year effect was less marked for PN berries. The berries were collected between veraison and maturity, separated in skin and pulp, and their content was analyzed by conventional analytical procedures and untargeted metabolomics. For anthocyanins, the relative quantitation was fairly comparable with both LC-MS determination and HPLC-DAD, which is a fully quantitative technique. The data show complex responses of the metabolite content (sugars, organic acids, amino acids, anthocyanins, flavonols, flavan-3-ols/procyanidins, stilbenes, hydroxycinnamic, and hydroxybenzoic acids) that depend on the rootstock, the scion, the vintage, the nitrogen level, the berry compartment. This opens a wide range of possibilities to adjust the content of these compounds through the choice of the roostock, variety and nitrogen fertilization.
authors.affiliation: UMR 1287, EGFV, Bordeaux Sciences Agro, Institt National de la Recherche Agronomique, Université de Bordeaux Villenave d'Ornon, France.
authors.forename: Aude
authors.initials: A
authors.lastname: Habran
authors.affiliation: Biotechnology Department, University of Verona Verona, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Commisso
authors.affiliation: UMR 1287, EGFV, Bordeaux Sciences Agro, Institt National de la Recherche Agronomique, Université de Bordeaux Villenave d'Ornon, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Helwi
authors.affiliation: UMR 1287, EGFV, Bordeaux Sciences Agro, Institt National de la Recherche Agronomique, Université de Bordeaux Villenave d'Ornon, France.
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Hilbert
authors.affiliation: Biotechnology Department, University of Verona Verona, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Negri
authors.affiliation: UMR 1287, EGFV, Bordeaux Sciences Agro, Institt National de la Recherche Agronomique, Université de Bordeaux Villenave d'Ornon, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Ollat
authors.affiliation: UMR 1287, EGFV, Bordeaux Sciences Agro, Institt National de la Recherche Agronomique, Université de Bordeaux Villenave d'Ornon, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Gomès
authors.affiliation: UMR 1287, EGFV, Bordeaux Sciences Agro, Institt National de la Recherche Agronomique, Université de Bordeaux Villenave d'Ornon, France.
authors.forename: Cornelis
authors.initials: C
authors.lastname: van Leeuwen
authors.affiliation: Biotechnology Department, University of Verona Verona, Italy.
authors.forename: Flavia
authors.initials: F
authors.lastname: Guzzo
authors.affiliation: UMR 1287, EGFV, Bordeaux Sciences Agro, Institt National de la Recherche Agronomique, Université de Bordeaux Villenave d'Ornon, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Delrot
keywords: berry; grapevine; metabolomics; nitrogen; rootstock