title: Genome-wide identification and characterization of TCP genes involved in ovule development of Phalaenopsis equestris.
abstract: TEOSINTE-BRANCHED/CYCLOIDEA/PCF (TCP) proteins are plant-specific transcription factors known to have a role in multiple aspects of plant growth and development at the cellular, organ and tissue levels. However, there has been no related study of TCPs in orchids. Here we identified 23 TCP genes from the genome sequence of Phalaenopsis equestris Phylogenetic analysis distinguished two homology classes of PeTCP transcription factor families: classes I and II. Class II was further divided into two subclasses, CIN and CYC/TB1. Spatial and temporal expression analysis showed that PePCF10 was predominantly expressed in ovules at early developmental stages and PeCIN8 had high expression at late developmental stages in ovules, with overlapping expression at day 16 after pollination. Subcellular localization and protein-protein interaction analyses revealed that PePCF10 and PeCIN8 could form homodimers and localize in the nucleus. However, PePCF10 and PeCIN8 could not form heterodimers. In transgenic Arabidopsis thaliana plants (overexpression and SRDX, a super repression motif derived from the EAR-motif of the repression domain of tobacco ETHYLENE-RESPONSIVE ELEMENT-BINDING FACTOR 3 and SUPERMAN, dominantly repressed), the two genes helped regulate cell proliferation. Together, these results suggest that PePCF10 and PeCIN8 play important roles in orchid ovule development by modulating cell division.
authors.affiliation: Institute of Tropical Plant Sciences, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Yu-Fu
authors.initials: YF
authors.lastname: Lin
authors.affiliation: Institute of Tropical Plant Sciences, National Cheng Kung University, Tainan, Taiwan Department of Life Sciences, National Cheng Kung University, Tainan, Taiwan.
authors.forename: You-Yi
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Department of Life Sciences, National Cheng Kung University, Tainan, Taiwan Orchid Research and Development Center, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Yu-Yun
authors.initials: YY
authors.lastname: Hsiao
authors.affiliation: Institute of Tropical Plant Sciences, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Ching-Yu
authors.initials: CY
authors.lastname: Shen
authors.affiliation: Orchid Research and Development Center, National Cheng Kung University, Tainan, Taiwan Shenzhen Key Laboratory for Orchid Conservation and Utilization, The National Orchid Conservation Center of China and The Orchid Conservation and Research Center of Shenzhen, Shenzhen, China.
authors.forename: Jui-Ling
authors.initials: JL
authors.lastname: Hsu
authors.affiliation: Division of Strategic Research and Development, Graduate School of Science and Engineering, Satitama University, Saitama, Japan.
authors.forename: Chuan-Ming
authors.initials: CM
authors.lastname: Yeh
authors.affiliation: Research Institute of Bioproduction, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Mitsuda
authors.affiliation: Division of Strategic Research and Development, Graduate School of Science and Engineering, Satitama University, Saitama, Japan Research Institute of Bioproduction, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Ohme-Takagi
authors.affiliation: Shenzhen Key Laboratory for Orchid Conservation and Utilization, The National Orchid Conservation Center of China and The Orchid Conservation and Research Center of Shenzhen, Shenzhen, China The Center for Biotechnology and BioMedicine, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China College of Forestry, South China Agricultural University, Guangzhou, China.
authors.forename: Zhong-Jian
authors.initials: ZJ
authors.lastname: Liu
authors.affiliation: Institute of Tropical Plant Sciences, National Cheng Kung University, Tainan, Taiwan Department of Life Sciences, National Cheng Kung University, Tainan, Taiwan Orchid Research and Development Center, National Cheng Kung University, Tainan, Taiwan tsaiwc@mail.ncku.edu.tw.
authors.forename: Wen-Chieh
authors.initials: WC
authors.lastname: Tsai
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D017343:Genes, Plant / Q000235:genetics* / Q000502:physiology; D055106:Genome-Wide Association Study; D017403:In Situ Hybridization; D029595:Orchidaceae / Q000235:genetics* / Q000254:growth & development; D056771:Ovule / Q000254:growth & development*; D010802:Phylogeny; D030821:Plants, Genetically Modified; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D020798:Two-Hybrid System Techniques
chemical_list: D014157:Transcription Factors
keywords: Cell proliferation; Phalaenopsis equestris; TEOSINTE-BRANCHED/CYCLOIDEA/PCF (TCP) proteins.; functional characterization; genome-wide identification; ovule development