title: In vitro BMP-2 peptide release from thiolated chitosan based hydrogel.
abstract: Thiolated chitosan based thermo-sensitive hydrogel is a water soluble system and the existing thiol groups are beneficial for the delivery of cysteine-rich peptides. In the present study, a kind of thiolated chitosan, i.e. chitosan-4-thio-butylamidine (CS-TBA) conjugate was characterized and used to prepare CS-TBA/hydroxyapatite (HA)/beta-glycerophosphate disodium (β-GP) thermo-sensitive hydrogel. The cysteine terminated peptide 24 (P24) containing residues 73-92 of the knuckle epitope of BMP-2 (N→C: KIPKASSVPTELSAISTLYLSGGC) was synthesized and characterized. The release behavior of P24 from CS-TBA based hydrogel was investigated in vitro. The thiol groups in CS-TBA may react with thiol groups in P24, thus decreases the P24 release rate and maintains the peptide release for a longer time compared with unmodified chitosan based hydrogel. Moreover, the bioactivity of P24 is preserved during release process. These results indicate that P24 loaded CS-TBA based thermosensitive hydrogel is a potential material for minimally invasive surgery of bone repair.
authors.affiliation: Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China; State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Xujie
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Qianli
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China. Electronic address: biomater@mail.tsinghua.edu.cn.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China; State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China. Electronic address: mi.shengli@sz.tsinghua.edu.cn.
authors.forename: Shengli
authors.initials: S
authors.lastname: Mi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D005260:Female; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats
chemical_list: D055396:Bone Morphogenetic Protein 2; D004337:Drug Carriers; D010446:Peptide Fragments; C493042:chitosan-4-thiobutylamidine; D002686:Chitin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: BMP-2 peptide release; Bone tissue engineering; Thiolated chitosan