title: Keratocystic odontogenic tumor overexpresses invadopodia-related proteins, suggesting invadopodia formation.
abstract: OBJECTIVE
  Keratocystic odontogenic tumor (KOT) is an odontogenic neoplasm that shows aggressive clinical behavior and local invasiveness. Invadopodia are actin-rich cellular protrusions exhibiting proteolytic pericellular activity, thereby inducing focal invasion in neoplastic cells and increasing neoplasms aggressiveness. Thus, this study aimed to evaluate immunoexpression of invadopodia-related proteins, cortactin, MT1-MMP, Tks4, and Tks5, in KOT.
  
  
  METHODS
  Immunohistochemistry of 16 cases of KOT, eight cases of calcifying cystic odontogenic tumor (CCOT), and eight samples of the oral mucosa (OM) was carried out to assess the expression of the above described invadopodia-related proteins in the basal and suprabasal layer.
  
  
  RESULTS
  KOT samples showed higher and significant immunoexpression of cortactin, MT1-MMP, TKs4, and TKs5 compared with the CCOT and OM samples. Significant expression of all these proteins was observed in the basal layer compared with the suprabasal layer in KOT.
  
  
  CONCLUSIONS
  Overexpression of cortactin, MT1-MMP, TKs4, and TKs5 was observed in KOT compared with samples of CCOT and OM. These proteins were also overexpressed in the basal over the suprabasal layer of KOT samples. Taken together, these results suggest the participation of invadopodia-related proteins on the pathogenesis of this lesion.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, University Center of Pará - CESUPA, Belém, Brazil; Department of Microbial Diseases, Eastman Dental Institute, University College London, London, England. Electronic address: Ribeiroalr@ig.com.br.
authors.forename: André Luis
authors.initials: AL
authors.lastname: Ribeiro Ribeiro
authors.affiliation: Cell Culture Laboratory, Faculty of Dentistry, Federal University of Pará, Belém, Brazil.
authors.forename: Natacha Malu Miranda
authors.initials: NM
authors.lastname: da Costa
authors.affiliation: School of Dentistry, Positivo University, Curitiba, Brazil.
authors.forename: Adriane Sousa
authors.initials: AS
authors.lastname: de Siqueira
authors.affiliation: Cell Culture Laboratory, Faculty of Dentistry, Federal University of Pará, Belém, Brazil.
authors.forename: Walessa
authors.initials: W
authors.lastname: Brasil da Silva
authors.affiliation: Cell Culture Laboratory, Faculty of Dentistry, Federal University of Pará, Belém, Brazil.
authors.forename: Maria Sueli
authors.initials: MS
authors.lastname: da Silva Kataoka
authors.affiliation: Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Ruy Gastaldoni
authors.initials: RG
authors.lastname: Jaeger
authors.affiliation: Cell Culture Laboratory, Faculty of Dentistry, Federal University of Pará, Belém, Brazil.
authors.forename: Sérgio
authors.initials: S
authors.lastname: de Melo Alves-Junior
authors.affiliation: Department of Microbial Diseases, Eastman Dental Institute, University College London, London, England.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Smith
authors.affiliation: Cell Culture Laboratory, Faculty of Dentistry, Federal University of Pará, Belém, Brazil.
authors.forename: João
authors.initials: J
authors.lastname: de Jesus Viana Pinheiro
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism; D051356:Cortactin / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009808:Odontogenic Tumors / Q000378:metabolism* / Q000473:pathology; D000069261:Podosomes / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D051356:Cortactin; C478582:SH3PXD2A protein, human; C547814:SH3PXD2B protein, human; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14