title: Zebrafish embryo tolerance to environmental stress factors-Concentration-dose response analysis of oxygen limitation, pH, and UV-light irradiation.
abstract: During the last century the increase in the mean global temperatures has been shown to impact on freshwater physicochemical parameters such as pH, dissolved oxygen, or ultraviolet (UV) light abundance. Changes in these parameters could modify the toxicity of environmental pollutants. Therefore, in the present study, the authors studied the tolerance (survival and sublethal endpoints) of zebrafish (Danio rerio) embryos to variations in pH (3-12), dissolved oxygen (3.9-237 μmol/L) and UV intensity (55-467 mW/m2) using selected endpoints. Sublethal endpoint assessment included the quantification of hatching success, developmental delay, reduction of body length, frequency of edema, and morphological abnormalities. Median lethal concentrations (LC50s; 96-h) of 3.68 and 10.21 were determined for acid and alkaline pH, respectively. Embryo survival appeared to be relatively resistant to oxygen depletion with a 96-h LC50 of 0.42 mg/L. However, concentrations of 6 mg/L and below caused edema and developmental retardations. Continuous exposure to UV radiation affected zebrafish development by reducing survival and hatching rate and triggering a series of developmental abnormalities such as pericardial edema and deformities. A 72-h LC50 of 227 mW/m2was derived from intensity-response modeling. By generation of concentration-response parameters the authors' data provide a basis for the subsequent assessment of combined effect of environmental stress parameters and chemicals. Environ Toxicol Chem 2017;36:682-690. © 2016 SETAC.
authors.affiliation: Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Thayres S
authors.initials: TS
authors.lastname: Andrade
authors.affiliation: Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Jorge F
authors.initials: JF
authors.lastname: Henriques
authors.affiliation: Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Ana Rita
authors.initials: AR
authors.lastname: Almeida
authors.affiliation: Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Amadeu M V M
authors.initials: AM
authors.lastname: Soares
authors.affiliation: Department of Bioanalytical Ecotoxicology, Helmholtz Centre for Environmental Research GmbH - UFZ, Leipzig, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Scholz
authors.affiliation: Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors.forename: Inês
authors.initials: I
authors.lastname: Domingues
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004361:Drug Tolerance; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D007928:Lethal Dose 50; D010100:Oxygen / Q000032:analysis* / Q000494:pharmacology; D014466:Ultraviolet Rays; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000254:growth & development
chemical_list: D014874:Water Pollutants, Chemical; D010100:Oxygen
keywords: Abiotic factors; Danio rerio; Dose response modeling; Global climate change; Stressors