title: Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
abstract: The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities. The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%. The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species. The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing. Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7. These results suggest that MSPS might be suitable for functional foods and natural drugs on preventing the ageing progress induced by toxic chemicals.
authors.affiliation: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, PR China.|College of Life Science, Shandong Agricultural University, Taian, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, PR China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Jing
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, PR China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Shandong Academy of Agricultural Science, Ji'nan, PR China.
authors.forename: Gen
authors.initials: G
authors.lastname: Che
authors.affiliation: Quality and safety Monitoring Center of Animal Products, Ji'nan, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhou
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Gao
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, PR China.
authors.forename: Shangshang
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, PR China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Ren
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Hao
authors.affiliation: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, PR China.
authors.forename: Le
authors.initials: L
authors.lastname: Jia
mesh_terms: D000375:Aging / Q000187:drug effects; D055362:Agrocybe / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D025282:Mycelium / Q000737:chemistry; D016566:Organoselenium Compounds / Q000302:isolation & purification* / Q000494:pharmacology*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D012643:Selenium / Q000032:analysis; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D009005:Monosaccharides; D016566:Organoselenium Compounds; D011134:Polysaccharides; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium