title: Plastic-Chip-Based Magnetophoretic Immunoassay for Point-of-Care Diagnosis of Tuberculosis.
abstract: Tuberculosis (TB) remains a relevant infectious disease in the 21st century, and its extermination is still far from being attained. Due to the extreme infectivity of incipient TB patients, a rapid sensing system for proficient point-of-care (POC) diagnostics is required. In our study, a plastic-chip-based magnetophoretic immunoassay (pcMPI) is introduced using magnetic and gold nanoparticles (NPs) modified with Mycobacterium tuberculosis (MTB) antibodies. This pcMPI offers an ultrasensitive limit of detection (LOD) of 1.8 pg·ml(-1) for the detection of CFP-10, an MTB-secreted antigen, as a potential TB biomarker with high specificity. In addition, by combining the plastic chip with an automated spectrophotometer setup, advantages include ease of operation, rapid time to results (1 h), and cost-effectiveness. Furthermore, the pcMPI results using clinical sputum culture filtrate samples are competitively compared with and integrated with clinical data collected from conventional tools such as the acid-fast bacilli (AFB) test, mycobacteria growth indicator tube (MGIT), polymerase chain reaction (PCR), and physiological results. CFP-10 concentrations were consistently higher in patients diagnosed with MTB infection than those seen in patients infected with nontuberculosis mycobacteria (NTM) (P < 0.05), and this novel test can distinguish MTB and NTM while MGIT cannot. All these results indicate that this pcMPI has the potential to become a new commercial TB diagnostic POC platform in view of its sensitivity, portability, and affordability.
authors.affiliation: Departments of Cogno-Mechatronics Engineering, Pusan National University , Busan 46241, Republic of Korea.
authors.forename: Jeonghyo
authors.initials: J
authors.lastname: Kim
authors.affiliation: Departments of Cogno-Mechatronics Engineering, Pusan National University , Busan 46241, Republic of Korea.
authors.forename: Minji
authors.initials: M
authors.lastname: Jang
authors.affiliation: Department of Nano Bio Research, National NanoFab Center (NNFC) , Daejeon 305-806, Republic of Korea.
authors.forename: Kyoung G
authors.initials: KG
authors.lastname: Lee
authors.affiliation: Department of Bacterial Respiratory Infections, Center for Infectious Diseases, National Institute of Health, Korea Center for Disease Control and Prevention , Cheongju 28159, Republic of Korea.
authors.forename: Kil-Soo
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Department of Nano Bio Research, National NanoFab Center (NNFC) , Daejeon 305-806, Republic of Korea.
authors.forename: Seok Jae
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Scinco R&D Center , 746 Daedeok-daero, Yuseong-gu, Daejeon 34055, Republic of Korea.
authors.forename: Kyung-Won
authors.initials: KW
authors.lastname: Ro
authors.affiliation: Scinco R&D Center , 746 Daedeok-daero, Yuseong-gu, Daejeon 34055, Republic of Korea.
authors.forename: In Sung
authors.initials: IS
authors.lastname: Kang
authors.affiliation: Scinco R&D Center , 746 Daedeok-daero, Yuseong-gu, Daejeon 34055, Republic of Korea.
authors.forename: Byung Do
authors.initials: BD
authors.lastname: Jeong
authors.affiliation: Department of Chemistry, Chung-Ang University , Seoul 06974, Republic of Korea.
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Park
authors.affiliation: Department of Microbiology and Research Institute for Medical Science, College of Medicine, Chungnam National University , Daejeon 35015, Republic of Korea.
authors.forename: Hwa-Jung
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Departments of Cogno-Mechatronics Engineering, Pusan National University , Busan 46241, Republic of Korea.
authors.forename: Jaebeom
authors.initials: J
authors.lastname: Lee
mesh_terms: D006046:Gold; D006801:Humans; D007118:Immunoassay; D053768:Metal Nanoparticles; D009169:Mycobacterium tuberculosis; D010969:Plastics; D019095:Point-of-Care Systems; D014376:Tuberculosis
chemical_list: D010969:Plastics; D006046:Gold
keywords: CFP-10; Mycobacterium tuberculosis (MTB); magnetophoretic immunoassay (MPI); mycobacteria growth indicator tube (MGIT); plastic chip; tuberculosis (TB)