title: A Partially Purified Acinetobacter baumannii Phage Preparation Exhibits no Cytotoxicity in 3T3 Mouse Fibroblast Cells.
abstract: A surge in the level and scale of antibiotic resistance has prompted renewed interest in the application of bacteriophages to treat bacterial infections. However, concerns still exist over their efficacy and safety. Acinetobacter baumannii phage BS46, a member of the family Myoviridae, has previously been shown to be effective in murine models. The cytotoxic effect of this phage was evaluated in mouse fibroblast 3T3 cells using four different assays: trypan blue; staining with Hoechst and propidium iodide; lactate dehydrogenase release; and the MTS assay. The addition of phage concentrations up to 2 × 10(9) pfu/mL showed little to no impact on the viability of 3T3 cells after 24 h exposure using the different assays. This study demonstrates that phage BS46 is non-cytotoxic to 3T3 cells using four different assays and that appropriate quality assurance protocols for phage therapeutics are required.
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Brighton University Brighton, UK.
authors.forename: Alexandra E
authors.initials: AE
authors.lastname: Henein
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Brighton University Brighton, UK.
authors.forename: Geoffrey W
authors.initials: GW
authors.lastname: Hanlon
authors.affiliation: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University Stockholm, Sweden.
authors.forename: Callum J
authors.initials: CJ
authors.lastname: Cooper
authors.affiliation: School of Pharmacy and Biomolecular Sciences, Brighton University Brighton, UK.
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Denyer
authors.affiliation: Cardiff School of Pharmacy and Pharmaceutical Sciences, Cardiff University Cardiff, UK.
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Maillard
keywords: Acinetobacter baumannii; MTS assay; bacteriophage; cytotoxicity; hoechst stain; lactate dehydrogenase; propidium iodide; trypan blue