title: An original method for producing acetaldehyde and diacetyl by yeast fermentation.
abstract: In this study a natural culture medium that mimics the synthetic yeast peptone glucose medium used for yeast fermentations was designed to screen and select yeasts capable of producing high levels of diacetyl and acetaldehyde. The presence of whey powder and sodium citrate in the medium along with manganese and magnesium sulfate enhanced both biomass and aroma development. A total of 52 yeasts strains were cultivated in two different culture media, namely, yeast peptone glucose medium and yeast acetaldehyde-diacetyl medium. The initial screening of the strains was based on the qualitative reaction of the acetaldehyde with Schiff's reagent (violet color) and diacetyl with Brady's reagent (yellow precipitate). The fermented culture media of 10 yeast strains were subsequently analyzed by gas chromatography to quantify the concentration of acetaldehyde and diacetyl synthesized. Total titratable acidity values indicated that a total titratable acidity of 5.5°SH, implying culture medium at basic pH, was more favorable for the acetaldehyde biosynthesis using strain D15 (Candida lipolytica; 96.05mgL-1acetaldehyde) while a total titratable acidity value of 7°SH facilitated diacetyl flavor synthesis by strain D38 (Candida globosa; 3.58mgL-1diacetyl). Importantly, the results presented here suggest that this can be potentially used in the baking industry.
authors.affiliation: "Petru Poni" Institute of Macromolecular Chemistry, Advanced Research Center for Bionanoconjugates and Biopolymers, Aleea GrigoreGhica Voda, Iasi, Romania.
authors.forename: Irina
authors.initials: I
authors.lastname: Rosca
authors.affiliation: "Petru Poni" Institute of Macromolecular Chemistry, Advanced Research Center for Bionanoconjugates and Biopolymers, Aleea GrigoreGhica Voda, Iasi, Romania. Electronic address: petrovici.anca@icmpp.ro.
authors.forename: Anca Roxana
authors.initials: AR
authors.lastname: Petrovici
authors.affiliation: "Petru Poni" Institute of Macromolecular Chemistry, Advanced Research Center for Bionanoconjugates and Biopolymers, Aleea GrigoreGhica Voda, Iasi, Romania.
authors.forename: Mihai
authors.initials: M
authors.lastname: Brebu
authors.affiliation: SC Zeelandia SRL, R&D Department, Valea Lupului, Iasi, Romania.
authors.forename: Irina
authors.initials: I
authors.lastname: Stoica
authors.affiliation: "Petru Poni" Institute of Macromolecular Chemistry, Advanced Research Center for Bionanoconjugates and Biopolymers, Aleea GrigoreGhica Voda, Iasi, Romania; "Grigore T. Popa" University of Medicine and Pharmacy, Iasi, Romania.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Minea
authors.affiliation: "Petru Poni" Institute of Macromolecular Chemistry, Advanced Research Center for Bionanoconjugates and Biopolymers, Aleea GrigoreGhica Voda, Iasi, Romania.
authors.forename: Narcisa
authors.initials: N
authors.lastname: Marangoci
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism*; D003931:Diacetyl / Q000378:metabolism*; D004734:Energy Metabolism; D005285:Fermentation; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D015003:Yeasts / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000079:Acetaldehyde; D003931:Diacetyl
keywords: Acetaldehyde; Diacetyl; YAD culture medium; Yeast fermentation