title: Phytotoxicity attenuation in Vigna radiata under heavy metal stress at the presence of biochar and N fixing bacteria.
abstract: This study assesses the effect of N-fixing bacteria and biochar synergism on plant growth and development of Vigna mungo under heavy metal stress (HM). Heavy metal stress is a worldwide problem, which causes critical effects on plant life due to oxidative stress. Application of biochar is a recent biological remediation technique, which often leads to an immobilization of heavy metals in soil. . Synergism of bacteria and biochar is a novel aspect to enhance plant growth under heavy metal stress. Woody biochar a byproduct of a dendro power industry was added as 1, 2.5 and 5% amounts combination with Bradyrhizobium japonicum, where mung seedlings were planted in serpentine soil rich in Ni, Mn, Cr and Co. Pot experiments were conducted for 12 weeks. The plant height, heavy metal uptake by plants, soil bioavailable heavy metal contents, soil N and P and microbial biomass carbon (MBC) were measured. The plant growth was enhanced with biochar amendment but a retardation was observed with high biochar application (5%). The soil N and P increased with the increase of biochar addition percentage while soil MBC showed reductions at 5% biochar amendment. Both soil bioavailable fractions of HM and up take of HMs by plants were gradually reduced with increase in biochar content. Based on the results, 2.5% biochar synergism with bacteria was the best for plant growth and soil nutrition status. Despite the synergism, available N was negatively correlated with the decrease of bioavailable metal percentage in soil whereas it was conversely for P.
authors.affiliation: Chemical and Environmental Systems Modeling Research Group, National Institute of Fundamental Studies, Kandy, Sri Lanka; Department of Botany, Faculty of Natural Sciences, Open University of Sri Lanka, Nawala, Nugegoda, Sri Lanka.
authors.forename: Mihiri
authors.initials: M
authors.lastname: Seneviratne
authors.affiliation: Chemical and Environmental Systems Modeling Research Group, National Institute of Fundamental Studies, Kandy, Sri Lanka.
authors.forename: Lakshika
authors.initials: L
authors.lastname: Weerasundara
authors.affiliation: Korea Biochar Research Center & School of Natural Resources and Environmental Science, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Yong Sik
authors.initials: YS
authors.lastname: Ok
authors.affiliation: University of Wuppertal, Soil- and Groundwater-Management, Pauluskirchstraße 7, 42285 Wuppertal, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Rinklebe
authors.affiliation: Chemical and Environmental Systems Modeling Research Group, National Institute of Fundamental Studies, Kandy, Sri Lanka. Electronic address: meththikavithanage@gmail.com.
authors.forename: Meththika
authors.initials: M
authors.lastname: Vithanage
mesh_terms: D001682:Biological Availability; D018533:Biomass; D020369:Bradyrhizobium / Q000502:physiology*; D002244:Carbon / Q000032:analysis; D002606:Charcoal; D019216:Metals, Heavy / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D009584:Nitrogen / Q000378:metabolism; D009586:Nitrogen Fixation; D010758:Phosphorus / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D013188:Sri Lanka; D000070658:Vigna / Q000187:drug effects* / Q000254:growth & development
chemical_list: D019216:Metals, Heavy; D012987:Soil; D012989:Soil Pollutants; C540010:biochar; D002606:Charcoal; D010758:Phosphorus; D002244:Carbon; D009584:Nitrogen
keywords: Biochar; Bradyrhizobium japonicum; Heavy metals; Microbial biomass carbon