title: Gardnerella vaginalis and Mollicute detection in rectal swabs from men who have sex with men.
abstract: The numbers of rectal sexually transmitted infections are on the rise especially among men who have sex with men. Males from men who have sex with men population are encouraged to send a rectal swab to the laboratory for sexually transmitted infection screening at their visit to the Genitourinary Medicine Clinic. In healthy asymptomatic males, the range of pathogens tested is limited therefore other pathogens may be left untreated allowing infections to persist among sexual partners. Molecular techniques have revolutionarised sexually transmitted infection testing enabling the detection of previously difficult-to-culture pathogens in extra-genital sites and have increased the evidence base for their clinical significance. The present study tests 107 rectal swabs from men who have sex with men negative for Chlamydia trachomatis and Neisseria gonorrhoeae against quantitative polymerase chain reaction (qPCR) assays targeting five common sexually transmitted bacteria which include Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum and Gardnerella vaginalis. The pathogenic role of these five bacteria in men who have sex with men is currently unknown. Amongst the 107 patients, a positive qPCR was obtained respectively for G. vaginalis 89 (83.2%); U. urealyticum 26 (24.3%); M. hominis 26 (24.3%); M. genitalium 10 (9.3%) and U. parvum 5 (4.7%). Bacterial loads in single and co-infections were compared for each organism. G. vaginalis and M. hominis loads were significantly ( p = 0.007 and p = 0.005, respectively) higher when co-infecting with at least one other organism. Amongst co-infections, the loads of each organism were assessed to determine possible synergies. G. vaginalis and M. hominis displayed a synergistic pattern ( r = 0.51; p = 0.02) which is in keeping with a similar synergy detected previously in the vagina of women with bacterial vaginosis. This study outlines that potential significant infections are being missed in men who have sex with men population; however, further research is warranted to confirm a pathogenesis in the rectal mucosa before routine screening can be introduced to clinical settings.
authors.affiliation: Regional Virus Laboratory, Belfast Health and Social Care Trust, Northern Ireland and Centre for Infection and Immunity, Queens University Belfast, Belfast, Northern Ireland.
authors.forename: Ciara
authors.initials: C
authors.lastname: Cox
authors.affiliation: Regional Virus Laboratory, Belfast Health and Social Care Trust, Northern Ireland and Centre for Infection and Immunity, Queens University Belfast, Belfast, Northern Ireland.
authors.forename: Alison P
authors.initials: AP
authors.lastname: Watt
authors.affiliation: Regional Virus Laboratory, Belfast Health and Social Care Trust, Northern Ireland and Centre for Infection and Immunity, Queens University Belfast, Belfast, Northern Ireland.
authors.forename: James P
authors.initials: JP
authors.lastname: McKenna
authors.affiliation: Regional Virus Laboratory, Belfast Health and Social Care Trust, Northern Ireland and Centre for Infection and Immunity, Queens University Belfast, Belfast, Northern Ireland.
authors.forename: Peter V
authors.initials: PV
authors.lastname: Coyle
mesh_terms: D000328:Adult; D005260:Female; D006194:Gardnerella vaginalis / Q000235:genetics* / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000453:epidemiology; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000453:epidemiology; D018451:Homosexuality, Male / Q000706:statistics & numerical data*; D006801:Humans; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D012007:Rectum / Q000382:microbiology*; D012749:Sexually Transmitted Diseases / Q000453:epidemiology; D008973:Tenericutes / Q000235:genetics* / Q000302:isolation & purification; D006113:United Kingdom / Q000453:epidemiology; D016585:Vaginosis, Bacterial / Q000175:diagnosis* / Q000453:epidemiology; D055815:Young Adult
keywords: G. vaginalis; MSM; Mollicutes; quantitative PCR; rectum; synergy