title: Brief Report: Differential Effects of Tenofovir, Abacavir, Emtricitabine, and Darunavir on Telomerase Activity In Vitro.
abstract: In vitro, tenofovir and abacavir induced a significant dose-dependent inhibition of telomerase activity at therapeutic concentrations in peripheral blood mononuclear cells of healthy subjects. Median inhibition of telomerase activity by tenofovir at 0.5 and 1 μM was 29% [Interquartile range (IQR) 29%-34%, P = 0.042] and 28% (IQR 28%-41%, P = 0.042), respectively. Abacavir inhibition was 12% (IQR 9%-13%, P = 0.043) at 3 μM and 14% (IQR 10%-29%, P = 0.043) at 10 μM. Tenofovir and abacavir did not change human telomerase reverse transcriptase (hTERT) levels or mRNA levels of other telomerase complex genes. Exposure to emtricitabine or darunavir did not affect telomerase activity, hTERT protein levels, or mRNA levels of telomerase/shelterin genes.
authors.affiliation: *Microbiology Service, Hospital Universitario La Paz, IdiPAZ, Madrid, Spain; †HIV Unit, Internal Medicine Service, Hospital Universitario La Paz, IdiPAZ, Madrid, Spain; ‡Instituto de Investigaciones Biomédicas CSIC/UAM, IdiPAZ, Biomarkers and New Therapies and CIBER de Enfermedades Raras (CIBERER), Madrid, Spain; and §Universidad de Alcalá de Henares, Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Stella-Ascariz
authors.forename: Rocío
authors.initials: R
authors.lastname: Montejano
authors.forename: Laura
authors.initials: L
authors.lastname: Pintado-Berninches
authors.forename: Susana
authors.initials: S
authors.lastname: Monge
authors.forename: José I
authors.initials: JI
authors.lastname: Bernardino
authors.forename: Ignacio
authors.initials: I
authors.lastname: Pérez-Valero
authors.forename: María L
authors.initials: ML
authors.lastname: Montes
authors.forename: Jesús
authors.initials: J
authors.lastname: Mingorance
authors.forename: Rosario
authors.initials: R
authors.lastname: Perona
authors.forename: José R
authors.initials: JR
authors.lastname: Arribas
mesh_terms: D017320:HIV Protease Inhibitors / Q000009:adverse effects*; D064368:Healthy Volunteers; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000201:enzymology*; D018894:Reverse Transcriptase Inhibitors / Q000009:adverse effects*; D019098:Telomerase / Q000037:antagonists & inhibitors*
chemical_list: D017320:HIV Protease Inhibitors; D018894:Reverse Transcriptase Inhibitors; D019098:Telomerase