title: Dangers resulting from DNA profiling of biological materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) with regard to forensic genetic analysis.
abstract: The study documents the risk that comes with DNA analysis of materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in forensic genetics. DNA chimerism was studied in 30 patients after allo-HSCT, based on techniques applied in contemporary forensic genetics, i.e. real-time PCR and multiplex PCR-STR with the use of autosomal DNA as well as Y-DNA markers. The results revealed that the DNA profile of the recipient's blood was identical with the donor's in the majority of cases. Therefore, blood analysis can lead to false conclusions in personal identification as well as kinship analysis. An investigation of buccal swabs revealed a mixture of DNA in the majority of recipients. Consequently, personal identification on the basis of stain analysis of the same origin may be impossible. The safest (but not ideal) material turned out to be the hair root. Its analysis based on autosomal DNA revealed 100% of the recipient's profile. However, an analysis based on Y-chromosome markers performed in female allo-HSCT recipients with male donors demonstrated the presence of donor DNA in hair cells - similarly to the blood and buccal swabs. In the light of potential risks arising from DNA profiling of biological materials derived from persons after allotransplantation in judicial aspects, certain procedures were proposed to eliminate such dangers. The basic procedures include abandoning the approach based exclusively on blood collection, both for kinship analysis and personal identification; asking persons who are to be tested about their history of allo-HSCT before sample collection and profile entry in the DNA database, and verification of DNA profiling based on hair follicles in uncertain cases.
authors.affiliation: Renata Jacewicz, Medical and Forensic Genetics Laboratory, Department of Forensic Medicine, Medical University of Lodz, Sędziowska 18 A, 91-304 Lodz, Poland, e-mail: renata.jacewicz@umed.lodz.pl.
authors.forename: R
authors.initials: R
authors.lastname: Jacewicz
authors.forename: K
authors.initials: K
authors.lastname: Lewandowski
authors.forename: J
authors.initials: J
authors.lastname: Rupa-Matysek
authors.forename: M
authors.initials: M
authors.lastname: Jędrzejczyk
authors.forename: J
authors.initials: J
authors.lastname: Berent
mesh_terms: D015415:Biomarkers / Q000032:analysis; D046528:Chimerism; D004247:DNA / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D053803:Forensic Genetics / Q000379:methods*; D014644:Genetic Variation; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D015415:Biomarkers; D004247:DNA