title: Single-cell multimodal profiling reveals cellular epigenetic heterogeneity.
abstract: Sample heterogeneity often masks DNA methylation signatures in subpopulations of cells. Here, we present a method to genotype single cells while simultaneously interrogating gene expression and DNA methylation at multiple loci. We used this targeted multimodal approach, implemented on an automated, high-throughput microfluidic platform, to assess primary lung adenocarcinomas and human fibroblasts undergoing reprogramming by profiling epigenetic variation among cell types identified through genotyping and transcriptional analysis.
authors.affiliation: Institute of Molecular and Cell Biology, Singapore.
authors.forename: Lih Feng
authors.initials: LF
authors.lastname: Cheow
authors.affiliation: Genome Institute of Singapore, Singapore.
authors.forename: Elise T
authors.initials: ET
authors.lastname: Courtois
authors.affiliation: Genome Institute of Singapore, Singapore.
authors.forename: Yuliana
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Institute of Molecular and Cell Biology, Singapore.
authors.forename: Ramya
authors.initials: R
authors.lastname: Viswanathan
authors.affiliation: Institute of Molecular and Cell Biology, Singapore.|School of Biological Sciences, Nanyang Technological University, Singapore.
authors.forename: Qiaorui
authors.initials: Q
authors.lastname: Xing
authors.affiliation: Bioinformatics Institute, Singapore.
authors.forename: Rui Zhen
authors.initials: RZ
authors.lastname: Tan
authors.affiliation: Genome Institute of Singapore, Singapore.|National Cancer Centre Singapore, Singapore.
authors.forename: Daniel S W
authors.initials: DS
authors.lastname: Tan
authors.affiliation: Genome Institute of Singapore, Singapore.|Department of Biological Sciences, National University of Singapore, Singapore.|The Jackson Laboratory for Genomic Medicine, Farmington, Connecticut, USA.
authors.forename: Paul
authors.identifier: 0000-0002-0191-3958
authors.initials: P
authors.lastname: Robson
authors.affiliation: Institute of Molecular and Cell Biology, Singapore.|Department of Biological Sciences, National University of Singapore, Singapore.
authors.forename: Yuin-Han
authors.initials: YH
authors.lastname: Loh
authors.affiliation: Departments of Bioengineering and Applied Physics, Stanford University, Stanford, California, USA.|Howard Hughes Medical Institute, Stanford, California, USA.
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Quake
authors.affiliation: Institute of Molecular and Cell Biology, Singapore.
authors.forename: William F
authors.initials: WF
authors.lastname: Burkholder
mesh_terms: D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D065150:Cellular Reprogramming / Q000235:genetics; D016172:DNA Fingerprinting; D019175:DNA Methylation / Q000235:genetics; D044127:Epigenesis, Genetic / Q000941:ethics*; D005347:Fibroblasts; D018740:Genetic Heterogeneity; D005819:Genetic Markers; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics; D046208:Microchip Analytical Procedures / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D005819:Genetic Markers