title: The Algal Meroterpene 11-Hydroxy-1'-O-Methylamentadione Ameloriates Dextran Sulfate Sodium-Induced Colitis in Mice.
abstract: Inflammatory bowel disease (IBD) is a complex class of immune disorders. Unfortunately, a treatment for total remission has not yet been found, while the use of natural product-based therapies has emerged as a promising intervention. The present study was aimed to investigate the anti-inflammatory effects of the algal meroterpene 11-hydroxy-1'-O-methylamentadione (AMT-E) in a murine model of dextran sodium sulphate (DSS)-induced colitis. AMT-E was orally administered daily (1, 10, and 20 mg/kg animal) to DSS treated mice (3% w/v) for 7 days. AMT-E prevented body weight loss and colon shortening and effectively attenuated the extent of the colonic damage. Similarly, AMT-E increased mucus production and reduced myeloperoxidase activity (marker for anti-inflammatory activity). Moreover, the algal meroterpene decreased the tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-10 levels, and caused a significant reduction of the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Our results demonstrate the protective effects of AMT-E on experimental colitis, provide an insight of the underlying mechanisms of this compound, and suggest that this class of marine natural products might be an interesting candidate for further studies on the prevention/treatment of IBD.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, Seville 41012, Spain. zbakh.h@hotmail.com.|Department of Biology, Faculty of Sciences, University Abdelmalek Essaadi, Tetouan 93030, Morocco. zbakh.h@hotmail.com.
authors.forename: Hanaa
authors.initials: H
authors.lastname: Zbakh
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, Seville 41012, Spain. etalero@us.es.
authors.forename: Elena
authors.initials: E
authors.lastname: Talero
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, Seville 41012, Spain. javila@us.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Avila
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, Seville 41012, Spain. antonioalcaide@hotmail.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Alcaide
authors.affiliation: Department of Organic Chemistry, Faculty of Marine and Environmental Sciences, University of Cádiz, Puerto Real (Cádiz) 11510, Spain. carolina.dereyes@uca.es.
authors.forename: Carolina
authors.initials: C
authors.lastname: de Los Reyes
authors.affiliation: Department of Organic Chemistry, Faculty of Marine and Environmental Sciences, University of Cádiz, Puerto Real (Cádiz) 11510, Spain. eva.zubia@uca.es.
authors.forename: Eva
authors.initials: E
authors.lastname: Zubía
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, Seville 41012, Spain. motilva@us.es.
authors.forename: Virginia
authors.initials: V
authors.lastname: Motilva
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy*; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016264:Dextran Sulfate / Q000009:adverse effects; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D016753:Interleukin-10 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D000459:Phaeophyceae / Q000737:chemistry*; D013729:Terpenes / Q000494:pharmacology / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015431:Weight Loss / Q000187:drug effects
chemical_list: C000619154:11-hydroxy-1'-O-methylamentadione; D000893:Anti-Inflammatory Agents; C556062:IL10 protein, mouse; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D013729:Terpenes; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D016264:Dextran Sulfate; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2
keywords: COX-2; cytokines; experimental colitis; iNOS; intestinal inflammation; macroalgae; marine meroterpenoids