title: New marine sterols from an algal-bearing gorgonian coral Pinnigorgia sp.
abstract: Four new marine sterols, (22E,24R)-ergosta-5,22-diene-3β,11α-diol (1), (24S)-ergosta-5-ene-3β,11α-diol (2), 5α,6α-epoxy-23-demethylgorgost-8-ene-3β,7α-diol (3), and 5α,6α-epoxy-23-demethylgorgost-8(14)-ene-3β,7α-diol (4), along with a known metabolite, 23-demethylgorgost-7-ene-3β,5α,6β-triol (5), were isolated from an algal-bearing gorgonian coral Pinnigorgia sp., collected off the waters of Taiwan. The structures of these sterols were elucidated on the basis of spectroscopic methods. Sterols 1-5 were tested for in vitro cytotoxicity in hepatic stellate cells (HSCs). Proliferation of HSCs plays a key role in the pathogenesis of liver fibrosis.
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 804, Taiwan; National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan.
authors.forename: Yu-Chia
authors.initials: YC
authors.lastname: Chang
authors.affiliation: Division of Neurosurgery, Department of Surgery, Kaohsiung Armed Forces General Hospital, Kaohsiung 802, Taiwan.
authors.forename: Nan-Fu
authors.initials: NF
authors.lastname: Chen
authors.affiliation: Graduate Institute of Natural Products, School of Traditional Chinese Medicine, College of Medicine and Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan 333, Taiwan; Research Center for Industry of Human Ecology and Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan.
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan; Division of Dentistry, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 813, Taiwan.
authors.forename: Chung-Chih
authors.initials: CC
authors.lastname: Tseng
authors.affiliation: Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan.
authors.forename: Tung-Ying
authors.initials: TY
authors.lastname: Wu
authors.affiliation: National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan.
authors.forename: Bo-Rong
authors.initials: BR
authors.lastname: Peng
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 804, Taiwan; Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wen
authors.affiliation: Department of Sport, Health, and Leisure, Cheng Shiu University, Kaohsiung 833, Taiwan.
authors.forename: Lee-Shing
authors.initials: LS
authors.lastname: Fang
authors.affiliation: Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan; School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan; Center for Molecular Medicine, China Medical University Hospital, Taichung 404, Taiwan; Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: yachwu@mail.cmu.edu.tw.
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 804, Taiwan; Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan. Electronic address: sheu@mail.nsysu.edu.tw.
authors.forename: Jyh-Horng
authors.initials: JH
authors.lastname: Sheu
authors.affiliation: National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan; Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan; Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan; Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan; Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: pjsung@nmmba.gov.tw.
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry* / Q000382:microbiology*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013261:Sterols / Q000737:chemistry*
chemical_list: D013261:Sterols
keywords: Gorgonian; HSCs; Marine sterol; Pinnigorgia