title: Osteogenic capacity of alkali-free bioactive glasses. In vitro studies.
abstract: The high alkali content bioactive glasses commonly used to regenerate bone in dental, orthopedic, and maxillofacial surgeries induce some cytotoxicity in surrounding tissues. The present study aims the ability of some alkali-free bioactive glasses compositions, recently developed by our research group, to stimulate human mesenchymal stem cells (hMSCs) differentiation into osteoblasts in comparison to 45S5 Bioglass®. The obtained results demonstrated that alkali-free bioactive glasses possess higher stimulating towards differentiation of hMSCs in comparison to the control 45S5 Bioglass®. The von Kossa assay demonstrated that all bioactive glasses studied were able to induce the appearance of calcium deposits even when the cells were cultured in DMEM, proving that these biomaterials per se induce hMSCs cell differentiation. It was also observed that in both cell culture medium used (DMEM, and osteogenesis differentiation medium) alkali-free bioactive glasses clearly induced the appearance of more calcium deposits than the 45S5 Bioglass®, indicating their greater ability to induce cell differentiation. In summary, these results indicate that alkali-free bioactive glasses are a safe and valid alternative to 45S5 Bioglass®. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2360-2365, 2017.
authors.affiliation: Reg4life - Regeneration Technology, S.A., Biocant, Parque Tecnológico de Cantanhede, 3060-197 Cantanhede, Portugal.
authors.forename: Ana F
authors.initials: AF
authors.lastname: Brito
authors.affiliation: Cell2B - Advanced Therapeutics, S.A., Biocant, Parque Tecnológico de Cantanhede, 3060-197 Cantanhede, Portugal.
authors.forename: Brígida
authors.initials: B
authors.lastname: Antunes
authors.affiliation: Cell2B - Advanced Therapeutics, S.A., Biocant, Parque Tecnológico de Cantanhede, 3060-197 Cantanhede, Portugal.
authors.forename: Francisco
authors.initials: F
authors.lastname: Dos Santos
authors.affiliation: Departamento de Engenharia de Materiais e Cerâmica, Universidade de Aveiro (UA), CICECO, 3810-193 Aveiro, Portugal.
authors.forename: Hugo R
authors.initials: HR
authors.lastname: Fernandes
authors.affiliation: Departamento de Engenharia de Materiais e Cerâmica, Universidade de Aveiro (UA), CICECO, 3810-193 Aveiro, Portugal.
authors.forename: José M F
authors.initials: JMF
authors.lastname: Ferreira
mesh_terms: D000328:Adult; D000468:Alkalies; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D002516:Ceramics / Q000493:pharmacokinetics*; D005260:Female; D005898:Glass; D006801:Humans; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects*
chemical_list: D000468:Alkalies; C425330:bioactive glass 45S5
keywords: 45S5 Bioglass®; alkali-free bioactive glasses; differentiation; hMSCs; proliferation