title: Podoplanin Expression Correlates with Disease Progression in Mycosis Fungoides.
abstract: The aim of this study was to investigate the role of lymphangiogenesis in the clinical progression and outcome of mycosis fungoides. Immunohistochemistry and Western blot techniques were used to assess the expression of podoplanin and vascular endothelial growth factor C in mycosis fungoides. Expression of vascular endothelial growth factor C measured by immunohistochemistry was significantly higher in mycosis fungoides samples in comparison with control cases (chronic benign dermatoses) (p = 0.0012). Increased expression of podoplanin was found in advanced vs. early mycosis fungoides (p < 0.0001), and was positively correlated with cutaneous and nodal involvement (p < 0.001, p < 0.0001; respectively). Higher podoplanin expression was also significantly associated with shorter survival (p < 0.001). Strong positive correlation was observed between expression of podoplanin analysed by immunohistochemistry and Western blot (r = 0.75, p < 0.0001). A similar association was shown regarding expression of vascular endothelial growth factor C (r = 0.68, p = 0.0007). In conclusion, these results suggest that increased expression of podoplanin is associated with poor clinical course, as well as shorter survival, of patients with mycosis fungoides.
authors.affiliation: Department of Dermatology, Venereology and Allergology, Wrocław Medical University, Chalubinskiego 1, PL-50-368 Wrocław, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Jankowska-Konsur
authors.forename: Christopher
authors.initials: C
authors.lastname: Kobierzycki
authors.forename: Jędrzej
authors.initials: J
authors.lastname: Grzegrzółka
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Piotrowska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gomulkiewicz
authors.forename: Natalia
authors.initials: N
authors.lastname: Glatzel-Plucinska
authors.forename: Adam
authors.initials: A
authors.lastname: Reich
authors.forename: Marzenna
authors.initials: M
authors.lastname: Podhorska-Okołów
authors.forename: Piotr
authors.initials: P
authors.lastname: Dzięgiel
authors.forename: Jacek C
authors.initials: JC
authors.lastname: Szepietowski
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D003593:Cytoplasm / Q000737:chemistry; D018450:Disease Progression; D042783:Endothelial Cells / Q000737:chemistry; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D042583:Lymphangiogenesis; D008207:Lymphatic Metastasis; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D009182:Mycosis Fungoides / Q000737:chemistry* / Q000473:pathology*; D009367:Neoplasm Staging; D012878:Skin Neoplasms / Q000737:chemistry* / Q000473:pathology*; D015996:Survival Rate; D013601:T-Lymphocytes / Q000737:chemistry; D042582:Vascular Endothelial Growth Factor C / Q000032:analysis*; D055815:Young Adult; D000098853:Podoplanin
chemical_list: D008562:Membrane Glycoproteins; D042582:Vascular Endothelial Growth Factor C; C117468:PDPN protein, human; D000098853:Podoplanin