title: Membrane Protein Crystallisation: Current Trends and Future Perspectives.
abstract: Alpha helical membrane proteins are the targets for many pharmaceutical drugs and play important roles in physiology and disease processes. In recent years, substantial progress has been made in determining their atomic structure using X-ray crystallography. However, a major bottleneck still remains; the identification of conditions that give crystals that are suitable for structure determination. Over the past 10 years we have been analysing the crystallisation conditions reported for alpha helical membrane proteins with the aim to facilitate a rational approach to the design and implementation of successful crystallisation screens. The result has been the development of MemGold, MemGold2 and the additive screen MemAdvantage. The associated analysis, summarised and updated in this chapter, has revealed a number of surprisingly successfully strategies for crystallisation and detergent selection.
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK.
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Parker
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK. simon.newstead@bioch.ox.ac.uk.
authors.forename: Simon
authors.initials: S
authors.lastname: Newstead
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D002021:Buffers; D011232:Chemical Precipitation; D003460:Crystallization; D018360:Crystallography, X-Ray; D003902:Detergents / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D017433:Protein Structure, Secondary; D012492:Salts / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D002021:Buffers; D003902:Detergents; D008565:Membrane Proteins; D012492:Salts
keywords: Crystallisation; Detergent selection; Membrane protein; Screen development