title: Live Imaging of Centriole Dynamics by Fluorescently Tagged Proteins in Starfish Oocyte Meiosis.
abstract: High throughput DNA sequencing, the decreasing costs of DNA synthesis, and universal techniques for genetic manipulation have made it much easier and quicker to establish molecular tools for any organism than it has been 5 years ago. This opens a great opportunity for reviving "nonconventional" model organisms, which are particularly suited to study a specific biological process and many of which have already been established before the era of molecular biology. By taking advantage of transcriptomics, in particular, these systems can now be easily turned into full fetched models for molecular cell biology.As an example, here we describe how we established molecular tools in the starfish Patiria miniata, which has been a popular model for cell and developmental biology due to the synchronous and rapid development, transparency, and easy handling of oocytes, eggs, and embryos. Here, we detail how we used a de novo assembled transcriptome to produce molecular markers and established conditions for live imaging to investigate the molecular mechanisms underlying centriole elimination-a poorly understood process essential for sexual reproduction of animal species.
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Joana
authors.initials: J
authors.lastname: Borrego-Pinto
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117, Heidelberg, Germany.
authors.forename: Kálmán
authors.initials: K
authors.lastname: Somogyi
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117, Heidelberg, Germany. lenart@embl.de.
authors.forename: Péter
authors.initials: P
authors.lastname: Lénárt
mesh_terms: D000818:Animals; D002502:Centrioles / Q000378:metabolism*; D030562:Databases, Protein; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008540:Meiosis; D057054:Molecular Imaging / Q000379:methods; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013215:Starfish / Q000166:cytology* / Q000502:physiology*; D064878:Web Browser
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins
keywords: Centriole elimination; Centrosome; Fluorescent markers; Live imaging; Meiosis; Microinjection; Starfish; Transcriptome