title: [Effect of Icaritin on K562 Cell Proliferation and Intracellular Reactive Oxygen Species].
abstract: OBJECTIVE
  To investigate the effect of icaritin (ICT) on proliferation of K562 cells and reactive oxygen species (ROS) in patients with chronic myeloid leukemia (CML).
  
  
  METHODS
  MTT assay was used to detect the effect of ICT on the proliferation of K562 cells. Flow cytometry was used to detect the apoptosis of K562 cells and intracellular ROS level. The expression of PARP protein was detected by Western blot.
  
  
  RESULTS
  ICT obviously inhibited the proliferation of K562 cells and induced their apoptosis, the expression of PARP protein was enhanced, and the intracellular ROS increased significantly.
  
  
  CONCLUSIONS
  The ICT showes the inhibitory effects on proliferation and apoptosis-inducing effects on K562 cells, and thier mechanism relats with the increase of reactive oxygen species in the cells.
authors.affiliation: Department of Hematology,The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.affiliation: Department of Hematology,The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Zi-Jian
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Department of Hematology,The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Wang
authors.affiliation: Department of Hematology,The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Hematology,The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Mu
authors.affiliation: Department of Hematology,The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lyu
authors.affiliation: Department of Hematology,The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.E-mail:xiyaming02@163.com.
authors.forename: Ya-Ming
authors.initials: YM
authors.lastname: Xi
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D005110:Extracellular Space; D005419:Flavonoids; D006801:Humans; D020014:K562 Cells; D017382:Reactive Oxygen Species
chemical_list: D005419:Flavonoids; D017382:Reactive Oxygen Species; C499403:icaritin