title: Calmodulin EF-hand peptides as Ca2+-switchable recognition tags.
abstract: Calmodulin is a representative calcium-binding protein comprised of four Ca2+-binding motifs with a helix-loop-helix structure (EF-hands). In this study, we clarified the potential of peptide segments derived from the third and fourth EF-hands (EF3 and EF4) to act as recognition tags. Through an analysis of the mode of disulfide formation among cysteines inserted at the N- or C-terminus of these peptide segments, EF3 and EF4 peptides were suggested to form a heterodimer with a topology similar to that in the wild-type protein. Heterodimer formation was shown to be a function of the Ca2+concentration, suggesting that these structures may be used as Ca2+-switchable recognition tags. An example of an "EF-tag" system involving the membrane fusion of liposomes decorated with EF3 and EF4 peptides is presented. © 2016 Wiley Periodicals, Inc. Biopolymers (Pept Sci), 2016.
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto, 611-0011, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Oku
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto, 611-0011, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Imanishi
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto, 611-0011, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Noshiro
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto, 611-0011, Japan.
authors.forename: Tomo
authors.initials: T
authors.lastname: Murayama
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto, 611-0011, Japan.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Takeuchi
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto, 611-0011, Japan.|Nanoscience and Nanotechnology Research Center, Research Organization for the 21st Century, Osaka Prefecture University, Naka-ku, Sakai, Osaka, 599-8570, Japan.
authors.forename: Ikuhiko
authors.initials: I
authors.lastname: Nakase
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto, 611-0011, Japan.
authors.forename: Shiroh
authors.initials: S
authors.lastname: Futaki
mesh_terms: D000595:Amino Acid Sequence; D002118:Calcium / Q000737:chemistry*; D002147:Calmodulin / Q000737:chemistry*; D019281:Dimerization; D020832:EF Hand Motifs; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D002147:Calmodulin; D008081:Liposomes; D010455:Peptides; D002118:Calcium
keywords: EF-hand; calcium; calmodulin; membrane fusion; recognition tag