title: Effect of long-term treatment with melatonin on vascular markers of oxidative stress/inflammation and on the anticontractile activity of perivascular fat in aging mice.
abstract: Some reports have suggested that inflammation in perivascular adipose tissue (PVAT) may be implicated in vascular dysfunction by causing the disappearance of an anticontractile effect. The aim of this study was to investigate the effects of chronic melatonin treatment on the functional responses of the small mesenteric arteries and on the expression of markers of inflammation/oxidative stress in the aortas of senescence-accelerated prone mice (SAMP8), a model of age-related vascular dysfunction. We investigated seven SAMP8 and seven control senescence-accelerated resistant mice (SAMR1) treated for 10 months with melatonin, as well as equal numbers of age-matched untreated SAMP8 and SAMR1. The mesenteric small resistance arteries were dissected and mounted on a wire myograph, and the concentration-response to norepinephrine was evaluated in vessels with intact PVAT and after the removal of the PVAT. The expression of markers of oxidative stress, inflammation and aging in the aortas was evaluated by immunostaining. In addition, the adiponectin content and the expression of adiponectin receptor 1 were evaluated in the visceral adipose tissue. In untreated SAMP8 mice, we observed an overexpression of oxidative stress and inflammatory markers in the vasculature compared with the controls. No anticontractile effect of the PVAT was observed in untreated SAMP8 mice. Long-term treatment of SAMP8 mice with melatonin increased the expression of some markers of vasoprotection, decreased oxidative stress and inflammation and restored the anticontractile effect of the PVAT. Decreased expression of adiponectin and adiponectin receptor 1 was also observed in visceral fat of untreated SAMP8, whereas a significant increase was observed after melatonin treatment.
authors.affiliation: Department of Clinical and Experimental Sciences, Clinica Medica, University of Brescia, Brescia, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Agabiti-Rosei
authors.affiliation: Anatomy and Physiopathology Division, Department of Clinical and Experimental Sciences, University of Brescia, Brescia, Italy.
authors.forename: Gaia
authors.initials: G
authors.lastname: Favero
authors.affiliation: Department of Clinical and Experimental Sciences, Clinica Medica, University of Brescia, Brescia, Italy.
authors.forename: Carolina
authors.initials: C
authors.lastname: De Ciuceis
authors.affiliation: Department of Clinical and Experimental Sciences, Clinica Medica, University of Brescia, Brescia, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Rossini
authors.affiliation: Department of Clinical and Experimental Sciences, Clinica Medica, University of Brescia, Brescia, Italy.
authors.forename: Enzo
authors.initials: E
authors.lastname: Porteri
authors.affiliation: Anatomy and Physiopathology Division, Department of Clinical and Experimental Sciences, University of Brescia, Brescia, Italy.
authors.forename: Luigi Fabrizio
authors.initials: LF
authors.lastname: Rodella
authors.affiliation: Anatomy and Physiopathology Division, Department of Clinical and Experimental Sciences, University of Brescia, Brescia, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Franceschetti
authors.affiliation: Department of Clinical and Experimental Sciences, Clinica Medica, University of Brescia, Brescia, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Maria Sarkar
authors.affiliation: Department of Clinical and Experimental Sciences, Clinica Medica, University of Brescia, Brescia, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Agabiti-Rosei
authors.affiliation: Department of Clinical and Experimental Sciences, Clinica Medica, University of Brescia, Brescia, Italy.|Istituto Clinico Città di Brescia, Division of Medicine, Brescia, Italy.
authors.forename: Damiano
authors.initials: D
authors.lastname: Rizzoni
authors.affiliation: Anatomy and Physiopathology Division, Department of Clinical and Experimental Sciences, University of Brescia, Brescia, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Rezzani
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D050152:Intra-Abdominal Fat / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D015415:Biomarkers; D008550:Melatonin