title: Diversity of apostome ciliates, Chromidina spp. (Oligohymenophorea, Opalinopsidae), parasites of cephalopods of the Mediterranean Sea.
abstract: Chromidina spp. are enigmatic apostome ciliates (Oligohymenophorea, Opalinopsidae) that parasitise the renal and pancreatic appendages of cephalopods. Only four species have been described, among which only three have been formally named. No DNA sequence has been reported so far. To investigate Chromidina spp. diversity, we sampled cephalopods in the Mediterranean Sea off Tunis, Tunisia, and identified two distinct Chromidina spp. in two different host species: Loligo vulgaris and Sepia officinalis. From haematoxylin-stained slides, we described morphological traits for these parasitic species and compared them to previous descriptions. We also re-described the morphology of Chromidina elegans (Foettinger, 1881) from Chatton and Lwoff's original materials and designated a neohapantotype and paraneohapantotypes for this species. We describe a new species, Chromidina chattoni Souidenne, Florent and Grellier n. sp., found in L. vulgaris off Tunisia, and evidence for a probable novel species, found in S. officinalis off Tunisia, although this latter species presents similarities to some morphological stages previously described for Chromidina cortezi Hochberg, 1971. We amplified, for the first time, an 18S rDNA marker for these two Chromidina species. Phylogenetic analysis supports the association of Chromidina within apostome ciliates. Genetic distance analysis between 18S rDNA sequences of representative apostomes indicates Pseudocollinia as the most closely related genus to Chromidina.
authors.affiliation: UMR 7245 CNRS MCAM, Muséum National d'Histoire Naturelle, Sorbonne Universités, CP 52, 57 rue Cuvier, 75005 Paris, France - UR03AGRO1 Ecosystèmes et Ressources Aquatiques, Institut National Agronomique de Tunisie, 43 avenue Charles Nicolle, 1082 Tunis, Tunisia.
authors.forename: Dhikra
authors.initials: D
authors.lastname: Souidenne
authors.affiliation: UMR 7245 CNRS MCAM, Muséum National d'Histoire Naturelle, Sorbonne Universités, CP 52, 57 rue Cuvier, 75005 Paris, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Florent
authors.affiliation: UMR 7245 CNRS MCAM, Muséum National d'Histoire Naturelle, Sorbonne Universités, CP 52, 57 rue Cuvier, 75005 Paris, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Dellinger
authors.affiliation: ISYEB, Institut de Systématique, Évolution, Biodiversité (UMR7205 CNRS, EPHE, MNHN, UPMC), Muséum National d'Histoire Naturelle, Sorbonne Universités, CP 51, 55 rue Buffon, 75005 Paris, France.
authors.forename: Jean Lou
authors.initials: JL
authors.lastname: Justine
authors.affiliation: UR03AGRO1 Ecosystèmes et Ressources Aquatiques, Institut National Agronomique de Tunisie, 43 avenue Charles Nicolle, 1082 Tunis, Tunisia.
authors.forename: Mohamed Salah
authors.initials: MS
authors.lastname: Romdhane
authors.affiliation: Department of Biology, Graduate School of Science, Osaka University, 1-1 Machikaneyama, 560-0043 Toyonaka, Osaka, Japan.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Furuya
authors.affiliation: UMR 7245 CNRS MCAM, Muséum National d'Histoire Naturelle, Sorbonne Universités, CP 52, 57 rue Cuvier, 75005 Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Grellier
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D049830:Cephalopoda / Q000469:parasitology*; D004275:DNA, Ribosomal / Q000737:chemistry; D005602:France; D016013:Likelihood Functions; D049834:Loligo / Q000469:parasitology; D008522:Mediterranean Sea; D016802:Oligohymenophorea / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D049833:Sepia / Q000469:parasitology; D016415:Sequence Alignment; D014416:Tunisia
chemical_list: D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S