title: CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma.
abstract: Melanoma is a very malignant disease and there are still no effective treatments. CD147 participates in the carcinogenesis of multiple human cancers and GLUT-1, as a glucose transporter, is associated with tumor growth. However, the function of CD147 and GLUT-1 in melanoma have not been completely understood. Thus, in this study we investigated the expression of CD147 and GLUT-1 in melanoma tissue, which were overexpressed compared with that in nevus tissue. In addition, CD147 and GLUT-1 were co-localized in the cytoplasm of human melanoma A375 cells. Immunoprecipitation proved that CD147 interacted with GLUT-1 at D105-199. Silencing CD147 by specific siRNA could downregulate GLUT-1 level via inhibiting PI3K/Akt signaling and decrease glucose uptake in A375 cells. In vivo experiments also supported that CD147 knockdown suppressed the tumor growth in melanoma subcutaneous mice model, observed by micro PET/CT. Our results could help validate CD147 as a new therapeutic target for treating melanoma.
authors.affiliation: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Su
authors.affiliation: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China.|Department of Dermatology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi.
authors.forename: Tianyuan
authors.initials: T
authors.lastname: Gao
authors.affiliation: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China.|Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Minghao
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Institute of Medical Science Research, Xiangya Hospital, Central South University, Hunan, China.
authors.forename: Lisha
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Zeng
authors.affiliation: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Peng
authors.affiliation: Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China.|Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D051926:Basigin / Q000235:genetics / Q000378:metabolism*; D063646:Carcinogenesis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D027342:Excitatory Amino Acid Transporter 2 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000378:metabolism* / Q000628:therapy; D051379:Mice; D008819:Mice, Nude; D009506:Nevus / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D023041:Xenograft Model Antitumor Assays
chemical_list: D027342:Excitatory Amino Acid Transporter 2; D034741:RNA, Small Interfering; D051926:Basigin; D019869:Phosphatidylinositol 3-Kinases; D005947:Glucose
keywords: CD147; GLUT-1; PI3K/Akt pathway; glycolysis; melanoma