title: Synchrotron FTIR microspectroscopy reveals early adipogenic differentiation of human mesenchymal stem cells at single-cell level.
abstract: Human mesenchymal stem cells (hMSCs) have been used as an ideal in vitro model to study human adipogenesis. However, little knowledge of the early stage differentiation greatly hinders our understanding on the mechanism of the adipogenesis processes. In this study, synchrotron radiation-based Fourier transform infrared (SR-FTIR) microspectroscopy was applied to track the global structural and compositional changes of lipids, proteins and nucleic acids inside individual hMSCs along the time course. The multivariate analysis of the SR-FTIR spectra distinguished the dynamic and significant changes of the lipids and nucleic acid at early differentiation stage. Importantly, changes of lipid structure during early days (Day 1-3) of differentiation might serve as a potential biomarker in identifying the state in early differentiation at single cell level. These results proved that SR-FTIR is a powerful tool to study the stem cell fate determination and early lipogenesis events.
authors.affiliation: Division of Physical Biology and CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences (CAS), Shanghai 201800, China; University of Chinese Academy of Science, Beijing 100049, China.
authors.forename: Zhixiao
authors.initials: Z
authors.lastname: Liu
authors.affiliation: National Center for Protein Science Shanghai, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, CAS, Shanghai 201210, China.
authors.forename: Yuzhao
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Cancer Center, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Canadian Light Source Inc. Saskatoon, Canada.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Cell Biology School of Medicine, Shandong University, Jinan 250012, China.
authors.forename: Zhaojian
authors.initials: Z
authors.lastname: Liu
authors.affiliation: National Center for Protein Science Shanghai, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, CAS, Shanghai 201210, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Division of Physical Biology and CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences (CAS), Shanghai 201800, China. Electronic address: hujun@sinap.ac.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Division of Physical Biology and CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences (CAS), Shanghai 201800, China. Electronic address: lujunhong@sinap.ac.cn.
authors.forename: Junhong
authors.initials: J
authors.lastname: Lü
mesh_terms: D017667:Adipocytes / Q000166:cytology; D050156:Adipogenesis; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D008055:Lipids / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D015591:Microspectrophotometry; D009696:Nucleic Acids / Q000032:analysis; D059010:Single-Cell Analysis / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D017356:Synchrotrons
chemical_list: D015415:Biomarkers; D008055:Lipids; D009696:Nucleic Acids
keywords: Adipogenic differentiation; Human mesenchymal stem cells; Synchrotron radiation-Fourier transform infrared spectroscopy