title: Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes.
abstract: Flow cytometry enables chromosomes to be sorted into different groups based on their characteristics, such as relative DNA content and the presence of repetitive DNA sequences. Despite the recent progress in the analysis of plant genome organization and chromosome structure, there is a need for easy methods to assign DNA sequences to individual chromosomes. Here, we describe an easy way to allocate genes or DNA sequences to chromosomes in wheat using flow-sorted chromosomes combined with fluorescence in situ hybridization and PCR analyses.
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Olomouc, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Cápal
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Olomouc, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Vrána
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Olomouc, Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Kubaláková
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Olomouc, Czech Republic. endo.takashi.2e@agr.ryukoku.ac.jp.|Faculty of Agriculture, Department of Plant Life Science, Ryukoku University, 1-5 Yokotani, Seta Oe-cho, Otsu, Shiga, 520-2194, Japan. endo.takashi.2e@agr.ryukoku.ac.jp.
authors.forename: Takashi R
authors.initials: TR
authors.lastname: Endo
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Olomouc, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Doležel
mesh_terms: D032461:Chromosomes, Plant; D005434:Flow Cytometry / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D014908:Triticum / Q000235:genetics*
keywords: Chromosomal allocation; Chromosome genomics; Chromosome sorting; FISH; Flow cytometry; Single chromosome amplification; Transgene; Wheat