title: Sex hormones establish a reserve pool of adult muscle stem cells.
abstract: Quiescent satellite cells, known as adult muscle stem cells, possess a remarkable ability to regenerate skeletal muscle following injury throughout life. Although they mainly originate from multipotent stem/progenitor cells of the somite, the mechanism underlying the establishment of quiescent satellite cell populations is unknown. Here, we show that sex hormones induce Mind bomb 1 (Mib1) expression in myofibres at puberty, which activates Notch signalling in cycling juvenile satellite cells and causes them to be converted into adult quiescent satellite cells. Myofibres lacking Mib1 fail to send Notch signals to juvenile satellite cells, leading to impaired cell cycle exit and depletion. Our findings reveal that the hypothalamic-pituitary-gonadal axis drives Mib1 expression in the myofibre niche. Moreover, the same axis regulates the re-establishment of quiescent satellite cell populations following injury. Our data show that sex hormones establish adult quiescent satellite cell populations by regulating the myofibre niche at puberty and re-establish them during regeneration.
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Ji-Hoon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Gi-Chan
authors.initials: GC
authors.lastname: Han
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Seo
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Inkuk
authors.initials: I
authors.lastname: Park
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Wookjin
authors.initials: W
authors.lastname: Park
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Jeong
authors.affiliation: Materials and Life Science Research Division, Korea Institute of Science and Technology, Seoul 136-791, South Korea.
authors.forename: Su Hyeon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Bae
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Jinwoo
authors.initials: J
authors.lastname: Seong
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Yum
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Sang-Hyeon
authors.initials: SH
authors.lastname: Hann
authors.affiliation: Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 120-752, South Korea.
authors.forename: Young-Guen
authors.initials: YG
authors.lastname: Kwon
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.|Center for RNA Research Institute for Basic Science, Seoul 151-742, South Korea.
authors.forename: Daekwan
authors.initials: D
authors.lastname: Seo
authors.affiliation: Materials and Life Science Research Division, Korea Institute of Science and Technology, Seoul 136-791, South Korea.
authors.forename: Man Ho
authors.initials: MH
authors.lastname: Choi
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-747, South Korea.
authors.forename: Young-Yun
authors.initials: YY
authors.lastname: Kong
mesh_terms: D053687:Adult Stem Cells / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D012739:Gonadal Steroid Hormones / Q000378:metabolism / Q000494:pharmacology*; D008822:Mice, Transgenic; D018485:Muscle Fibers, Skeletal / Q000166:cytology*; D018482:Muscle, Skeletal / Q000166:cytology; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics
chemical_list: D012739:Gonadal Steroid Hormones; C470629:MIB1 protein, mouse; D044767:Ubiquitin-Protein Ligases