title: Serum microRNA-135a downregulation as a prognostic marker of non-small cell lung cancer.
abstract: Abnormal expression of microRNA-135a (miR-135a) is closely associated with oncogenesis. However, the relationship between serum miR-135a levels and the clinical parameters and prognosis of non-small cell lung cancer (NSCLC) remain unclear. The study aimed to investigate the clinical significance of serum miR-135a expression in patients with NSCLC. miR-135a expression was analyzed by real-time PCR and its correlation with NSCLC was determined by various statistical methods for 104 NSCLC patients and 40 healthy volunteers. The serum miR-135a level was significantly lower in NSCLC patients than in healthy control subjects (P < 0.01), and was closely related to distant metastasis (P < 0.015), lymphatic metastasis (P = 0.000), TNM (tumor node metastasis) stage (P = 0.001), and pathological stage (P = 0.021) of NSCLC. The five year overall survival was significantly lower in patients with low miR-135a expression than that in patients with high serum miR-135a levels (P = 0.010). Multivariate analysis showed that serum miR-135a level could be treated as an independent risk factor for NSCLC prognosis (P = 0.011). In conclusion, the serum miR-135a level was downregulated in NSCLC patients, and was associated with poor prognosis. Additionally, it can be used as a biomarker for NSCLC prognosis.
authors.affiliation: Department of Thoracic Surgery, Taian City Central Hospital, Taian, Shandong, China.
authors.forename: Y K
authors.initials: YK
authors.lastname: Zhang
authors.affiliation: Department of Chest Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: B
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Thoracic Surgery, General Hospital of Jinan Military Command, Jinan, Shandong, China suigangl@sina.com.
authors.forename: G
authors.initials: G
authors.lastname: Sui
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000401:mortality; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000097:blood* / Q000401:mortality; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; C544965:MIRN135 microRNA, human; D035683:MicroRNAs