title: Genome sequencing of a single tardigrade Hypsibius dujardini individual.
abstract: Tardigrades are ubiquitous microscopic animals that play an important role in the study of metazoan phylogeny. Most terrestrial tardigrades can withstand extreme environments by entering an ametabolic desiccated state termed anhydrobiosis. Due to their small size and the non-axenic nature of laboratory cultures, molecular studies of tardigrades are prone to contamination. To minimize the possibility of microbial contaminations and to obtain high-quality genomic information, we have developed an ultra-low input library sequencing protocol to enable the genome sequencing of a single tardigrade Hypsibius dujardini individual. Here, we describe the details of our sequencing data and the ultra-low input library preparation methodologies.
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan.|Systems Biology Program, Graduate School of Media and Governance, Keio University, Tsuruoka, Yamagata 997-0052, Japan.
authors.forename: Kazuharu
authors.initials: K
authors.lastname: Arakawa
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan.|Systems Biology Program, Graduate School of Media and Governance, Keio University, Tsuruoka, Yamagata 997-0052, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yoshida
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan.|Systems Biology Program, Graduate School of Media and Governance, Keio University, Tsuruoka, Yamagata 997-0052, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
mesh_terms: D000818:Animals; D015723:Gene Library; D016678:Genome; D007448:Invertebrates / Q000145:classification*; D010802:Phylogeny; D058467:Tardigrada / Q000145:classification*