title: Hyperbaric oxygen treatment suppresses withdrawal signs in morphine-dependent mice.
abstract: Hyperbaric oxygen (HBO2) therapy reportedly reduces opiate withdrawal in human subjects. The purpose of this research was to determine whether HBO2 treatment could suppress physical signs of withdrawal in opiate-dependent mice. Male NIH Swiss mice were injected s.c. with morphine sulfate twice a day for 4 days, the daily dose gradually increasing from 50mg/kg on day 1 to 125mg/kg on day 4. On day 5, withdrawal was precipitated by i.p. injection of 5.0mg/kg naloxone. Mice were observed for physical withdrawal signs, including jumping, forepaw tremor, wet-dog shakes, rearing and defecation for 30min. Sixty min prior to the naloxone injection, different groups of mice received either a 30-min or 60-min HBO2 treatment at 3.5atm absolute. HBO2 treatment significantly reduced naloxone-precipitated jumping, forepaw tremor, wet-dog shakes, rearing and defecation. Based on these experimental findings, we concluded that treatment with HBO2 can suppress physical signs of withdrawal syndrome in morphine-dependent mice.
authors.affiliation: Department of Psychology, Washington State University, Pullman, WA, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Nicoara
authors.affiliation: Department of Integrative Physiology and Neuroscience, Washington State University, Pullman, WA, USA.
authors.forename: Yangmiao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Psychology, Washington State University, Pullman, WA, USA.
authors.forename: Jordan T
authors.initials: JT
authors.lastname: Nelson
authors.affiliation: Department of Psychology, Washington State University, Pullman, WA, USA.
authors.forename: Abigail L
authors.initials: AL
authors.lastname: Brewer
authors.affiliation: Department of Integrative Physiology and Neuroscience, Washington State University, Pullman, WA, USA.
authors.forename: Prianka
authors.initials: P
authors.lastname: Maharaj
authors.affiliation: Department of Psychology, Washington State University, Pullman, WA, USA.
authors.forename: Shea N
authors.initials: SN
authors.lastname: DeWald
authors.affiliation: Department of Physiology and Pharmacology, University of the Pacific Thomas J. Long School of Pharmacy and Health Sciences, Stockton, CA, USA.
authors.forename: Donald Y
authors.initials: DY
authors.lastname: Shirachi
authors.affiliation: Department of Psychology, Washington State University, Pullman, WA, USA; Department of Integrative Physiology and Neuroscience, Washington State University, Pullman, WA, USA; Translational Addiction Research Center, Washington State University, Pullman, WA, USA. Electronic address: quockr@wsu.edu.
authors.forename: Raymond M
authors.initials: RM
authors.lastname: Quock
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D006931:Hyperbaric Oxygenation; D008297:Male; D051379:Mice; D009020:Morphine / Q000008:administration & dosage*; D009270:Naloxone / Q000008:administration & dosage; D009294:Narcotics / Q000008:administration & dosage*; D013375:Substance Withdrawal Syndrome / Q000517:prevention & control*
chemical_list: D009294:Narcotics; D009270:Naloxone; D009020:Morphine
keywords: Hyperbaric oxygen; Morphine; Mouse; Opiate withdrawal; Physical dependence