title: Therapeutic Potential of Adipose-derived Stem Cell-based Microtissues in a Rat Model of Stress Urinary Incontinence.
abstract: To examine the potential and mechanism of 3-dimensional cultures of adipose-derived stem cells (ADSCs) in the treatment of stress urinary incontinence (SUI) in a rat model simulating menopause combined with preceding childbirth injury.
  
  
  
  ADSCs were used to generate microtissues (MTs) with a hanging drop method. Forty-eight postpartum Sprague-Dawley rats were developed as SUI models after 4 hours of vagina dilation followed by bilateral ovariectomy. Ten rats that underwent sham ovariectomy without vagina dilation served as the control group. The SUI rats were divided into 3 groups and received urethral injection of phosphate-buffered saline, ADSCs, and MTs. Specimens were harvested for histology examination and ADSCs tracking at days 1, 3, 7, and 28 (n = 3) postinjection. At day 28, the remaining rats were examined for voiding function. Western blot, immunofluorescence, and immunohistochemistry staining were performed to examine histological changes and cytokine expression.
  
  
  
  The voiding function and histopathological structures were better recovered in the MT group than in the ADSC group. Compared with ADSCs, MTs express higher level of vascular endothelial growth factor and TNFα-stimulated gene/protein 6 in vitro, and represented a higher retention rate in vivo.
  
  
  
  Urethral injection of MTs better restored voiding function than ADSCs.
authors.affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: General Hospital of Ningxia Medical University, Yinchuan, China.
authors.forename: Guangyong
authors.initials: G
authors.lastname: Li
authors.affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, China.
authors.forename: Hongen
authors.initials: H
authors.lastname: Lei
authors.affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, China.
authors.forename: Ruili
authors.initials: R
authors.lastname: Guan
authors.affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, China.
authors.forename: Bicheng
authors.initials: B
authors.lastname: Yang
authors.affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, China.
authors.forename: Zhezhu
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Department of Urology, First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hui
authors.affiliation: General Hospital of Ningxia Medical University, Yinchuan, China.
authors.forename: Fubao
authors.initials: F
authors.lastname: Chen
authors.affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, China. Electronic address: xinzc@bjmu.edu.cn.
authors.forename: Zhongcheng
authors.initials: Z
authors.lastname: Xin
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008593:Menopause; D051381:Rats; D017207:Rats, Sprague-Dawley; D018874:Spheroids, Cellular / Q000378:metabolism / Q000637:transplantation*; D033581:Stem Cell Transplantation; D013234:Stem Cells; D046509:Tissue Culture Techniques; D016378:Tissue Transplantation; D016211:Transforming Growth Factor alpha / Q000235:genetics / Q000378:metabolism; D014521:Urethra / Q000000981:diagnostic imaging / Q000473:pathology; D014550:Urinary Incontinence, Stress / Q000628:therapy*; D014554:Urination; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D016211:Transforming Growth Factor alpha; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat