title: The mitochondrial and endoplasmic reticulum pathways involved in the apoptosis of bursa of Fabricius cells in broilers exposed to dietary aflatoxin B1.
abstract: Aflatoxin B1 (AFB1), a toxic metabolite produced by some fungi, exerts well-known hepatocarcinogenic and immunosuppressive effects, the latter can increase the apoptotic immune cells in vitro. However, it is largely unknown that which signaling pathways contribute to excessive apoptosis of immune cells which induced by AFB1. In this study, we investigated the roles of the mitochondria, endoplasmic reticulum (ER) and death receptor activated apoptotic pathways in the bursal of Fabricius (BF) cells in the broilers exposed to AFB1 diet. We found that (1) AFB1 diet induced morphological changes in the BF. (2) FCM and TUNEL methods showed that excessive apoptosis could be resulted from AFB1 intake. (3) AFB1-induced apoptosis of bursal cells involved mitochondrial pathway (increase of Bax, Bak, cytC, caspase-9, Apaf-1, caspase-3 and decrease of Bcl-2 and Bcl-xL) and ER pathway (increase of Grp78/Bip, Grp94 and CaM). (4) Oxidative stress was confirmed in the BF of chicken fed on AFB1 diet. Overall, this work is the first to demonstrate that the activation of mitochondria and ER apoptosis pathways can lead to excessive apoptosis in BF cells, and oxidative stress is a crucial driver during AFB1 exposure.
authors.affiliation: Department of Wild Animal Disease, College of Life Science, China West Normal University, Nanchong 637009, Sichuan, The People's Republic of China.|Key Laboratory of Southwest China Wildlife Resources Conservation (China West Normal University), Ministry of Education, Nanchong 637009, Sichuan, The People's Republic of China.
authors.forename: Shibin
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Department of Wild Animal Disease, College of Life Science, China West Normal University, Nanchong 637009, Sichuan, The People's Republic of China.|Key Laboratory of Southwest China Wildlife Resources Conservation (China West Normal University), Ministry of Education, Nanchong 637009, Sichuan, The People's Republic of China.
authors.forename: Bangyuan
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Animal Pathlogy, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, Sichuan, The People's Republic of China.
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Animal Pathlogy, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, Sichuan, The People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Animal Pathlogy, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, Sichuan, The People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Liang
authors.affiliation: Department of Wild Animal Disease, College of Life Science, China West Normal University, Nanchong 637009, Sichuan, The People's Republic of China.|Key Laboratory of Southwest China Wildlife Resources Conservation (China West Normal University), Ministry of Education, Nanchong 637009, Sichuan, The People's Republic of China.
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Wild Animal Disease, College of Life Science, China West Normal University, Nanchong 637009, Sichuan, The People's Republic of China.|Key Laboratory of Southwest China Wildlife Resources Conservation (China West Normal University), Ministry of Education, Nanchong 637009, Sichuan, The People's Republic of China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Wild Animal Disease, College of Life Science, China West Normal University, Nanchong 637009, Sichuan, The People's Republic of China.|Key Laboratory of Southwest China Wildlife Resources Conservation (China West Normal University), Ministry of Education, Nanchong 637009, Sichuan, The People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002060:Bursa of Fabricius / Q000187:drug effects* / Q000378:metabolism; D002645:Chickens; D004032:Diet; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
chemical_list: D016604:Aflatoxin B1
keywords: aflatoxin B1; apoptosis; broiler; bursa of Fabricius; oxidative stress