title: Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats.
abstract: The aim of this study was to investigate the involvement of androgen, mainly testosterone, in the expression of renal senescence marker protein-30 (SMP30) in male rats. We found that the renal SMP30 expression was up-regulated by endogenous testosterone stimulation during puberty. Interestingly, androgen-deficient orchidectomized (ORX) rats exhibited lower SMP30 mRNA and protein expression in the kidney, and that was restored by testosterone propionate (TP) replacement. Abrogation of androgen receptor (AR) activity by co-treatment with flutamide abolished testosterone-induced SMP30 expression in the kidney as well as in the NRK52E cells. However, SMP30 expression was unaltered in the liver of ORX rats. We also showed a positive correlation between renal SMP30 expression and plasma testosterone level during the aging process. TP-induced SMP30 expression in ovariectomized (OVX) rats was observed and was an evidence to explain the gender difference of SMP30 levels. Immunofluorescence assay showed that renal SMP30 was specifically expressed in the proximal tubular segments of the kidney. The urinary Ca(2+) level was increased in both ORX and male aging rats. Taken together, our results indicate a novel role of testosterone in regulating SMP30 expression specifically in the kidney to contribute to urinary calcium absorption.
authors.affiliation: Institute and Department of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China.
authors.forename: Po-Han
authors.initials: PH
authors.lastname: Lin
authors.affiliation: Institute and Department of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China.
authors.forename: Cai-Yun
authors.initials: CY
authors.lastname: Jian
authors.affiliation: Medical Center of Aging Research, China Medical University Hospital, Taichung 40402, Taiwan, Republic of China.
authors.forename: Jou-Chun
authors.initials: JC
authors.lastname: Chou
authors.affiliation: Institute and Department of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China.
authors.forename: Chien-Wei
authors.initials: CW
authors.lastname: Chen
authors.affiliation: Institute and Department of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China.
authors.forename: Chih-Chieh
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Rehabilitation, Cheng Hsin General Hospital, Taipei 11212, Taiwan, Republic of China.
authors.forename: Christina
authors.initials: C
authors.lastname: Soong
authors.affiliation: Aesthetic Medical Center, Department of Dermatology, Chang Gung Memorial Hospital, Taoyuan 33378, Taiwan, Republic of China.|Department of Medicine, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan, Republic of China.
authors.forename: Sindy
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Rehabilitation, Cheng Hsin General Hospital, Taipei 11212, Taiwan, Republic of China.
authors.forename: Fu-Kong
authors.initials: FK
authors.lastname: Lieu
authors.affiliation: Institute and Department of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, Republic of China.|Medical Center of Aging Research, China Medical University Hospital, Taichung 40402, Taiwan, Republic of China.|Department of Biotechnology, College of Health Science, Asia University, Taichung 41354, Taiwan, Republic of China.|Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 11217, Taiwan, Republic of China.
authors.forename: Paulus S
authors.initials: PS
authors.lastname: Wang
authors.affiliation: Aesthetic Medical Center, Department of Dermatology, Chang Gung Memorial Hospital, Taoyuan 33378, Taiwan, Republic of China.|Department of Physiology and Pharmacology, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan, Republic of China.
authors.forename: Shyi-Wu
authors.initials: SW
authors.lastname: Wang
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000652:urine*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases; D005260:Female; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009919:Orchiectomy; D010052:Ovariectomy; D011627:Puberty; D017207:Rats, Sprague-Dawley; D011944:Receptors, Androgen / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism*; D043343:Testosterone Propionate / Q000494:pharmacology
chemical_list: D002135:Calcium-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011944:Receptors, Androgen; D013739:Testosterone; D002265:Carboxylic Ester Hydrolases; C513325:Rgn protein, rat; D000252:Calcium-Transporting ATPases; D002118:Calcium; D043343:Testosterone Propionate