title: Multi-channel cell co-culture for drug development based on glass microfluidic chip-mass spectrometry coupled platform.
abstract: Cell-based drug assay plays an essential role in drug development. By coupling a microfluidic chip with mass spectrometry (MS), we developed a multifunctional platform. Cell co-culture, cell apoptosis assay, fluorescence and MS detection of intracellular drug absorption could be simultaneously conducted on this platform.
  
  
  
  Three micro-channels were fabricated through photolithography technology to conduct the cell co-culture. Cell apoptosis after drug treatment was assayed by fluorescent probes (Hoechst 33342). Intracellular Dox absorption was analyzed by confocal fluorescent microscopy. With a high voltage (~ 4.5 kV) applied onto the microfluidic chip, the ionization spray was successfully generated by dropping isopropanol onto it. By coupling with a Shimadzu LCMS-2010 A mass spectrometer, intracellular CPA absorption was detected on the microfluidic chip.
  
  
  
  The microfluidic chip-MS coupled platform showed high biocompatibility. Distinction of cell apoptosis between co-cultured and mono-cultured cells was detected. The results of intracellular drug absorption well explained the different cell apoptosis rate.
  
  
  
  Cell-based drug assay was facilely and successfully conducted on the microfluidic chip-MS coupled platform. This technology we have devised could promote MS application in the field of drug development. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: School of Science, China University of Geosciences (Beijing), Beijing, 100083, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, China.
authors.forename: Mingsha
authors.initials: M
authors.lastname: Jie
authors.affiliation: School of Science, China University of Geosciences (Beijing), Beijing, 100083, China.
authors.forename: Xueling
authors.initials: X
authors.lastname: Dong
authors.affiliation: School of Science, China University of Geosciences (Beijing), Beijing, 100083, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Qi
authors.affiliation: Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, China.
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Lin
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D018920:Coculture Techniques / Q000295:instrumentation / Q000379:methods*; D003520:Cyclophosphamide / Q000032:analysis / Q000494:pharmacology; D004317:Doxorubicin / Q000032:analysis / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005453:Fluorescence; D056945:Hep G2 Cells; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D056656:Lab-On-A-Chip Devices; D061986:MCF-7 Cells; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D004317:Doxorubicin; D003520:Cyclophosphamide