title: Dysregulation of microRNA-128 expression in WHO grades 2 glioma is associated with glioma-associated epilepsy: Down-regulation of miR-128 induces glioma-associated seizure.
abstract: Approximately 80% of glioma patients will experience at least one seizure activity during the course of the disease, and because the etiology of glioma-related seizure is most likely multifactorial and complex, it remains poorly understood. MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators. MicroRNA-128 was found to be decreased in glioblastoma, and knockout of the microRNA-128a gene could induce epilepsy in mice. Based on the Chinese Glioma Genome Atlas and previous study, we hypothesized that dysregulation of miR-128 expression may play a role in the pathogenesis of TAE in low-grade glioma.
  
  
  
  Fifty-three low-grade glioma samples were analyzed for the expression levels of miR-128 using qRT-PCR, and candidate targets of miR-128 (Cacnge2, GRIK3, and GRIN2D) were detected by the 3'-UTR luciferase reporter assay. Four other miRs (miR-9, miR-192a, miR-92a, and miR-451) that showed dysregulation of glioblastoma in the CGGA data were also analyzed.
  
  
  
  The microRNA-128 expression levels were down-regulated in low-grade glioma tissue (t-test; p=0.009). Dysregulation of miR-128 expression in low-grade glioma is associated with glioma-associated epilepsy (p=0.006). No statistical significance of miR-9, miR-192a, miR-92a, and miR-451 was found to be associated with LGG.
  
  
  
  Our results here, together with other recent lines of evidence, indicate that miR-128 is an extremely attractive target for therapy in glioma patients with seizure.
authors.affiliation: Department of Neurosurgery, West China Hospital, Si Chuan University, Chengdu, 610041, China. Electronic address: 76896489@qq.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Neurosurgery, West China Hospital, Si Chuan University, Chengdu, 610041, China. Electronic address: 4589235@qq.com.
authors.forename: Wang
authors.initials: W
authors.lastname: Xiang
authors.affiliation: Department of Neurosurgery, West China Hospital, Si Chuan University, Chengdu, 610041, China. Electronic address: 827005432@qq.com.
authors.forename: Liu
authors.initials: L
authors.lastname: Yanhui
authors.affiliation: Department of Neurosurgery, West China Hospital, Si Chuan University, Chengdu, 610041, China. Electronic address: 466230959@qq.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Ruofei
authors.affiliation: West China Medical School of Si Chuan University, Chengdu, 610041, China. Electronic address: 297913442@qq.com.
authors.forename: Mao
authors.initials: M
authors.lastname: Yunhe
authors.affiliation: Department of Neurosurgery, West China Hospital, Si Chuan University, Chengdu, 610041, China. Electronic address: 644925826@qq.com.
authors.forename: Luo
authors.initials: L
authors.lastname: Jiewen
authors.affiliation: Department of Neurosurgery, West China Hospital, Si Chuan University, Chengdu, 610041, China. Electronic address: qingmao2000@163.com.
authors.forename: Mao
authors.initials: M
authors.lastname: Qing
mesh_terms: D000328:Adult; D001921:Brain / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D015536:Down-Regulation; D004827:Epilepsy / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D005910:Glioma / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D060787:Neoplasm Grading; D014944:World Health Organization
chemical_list: C545189:MIRN128 microRNA, human; C546783:MIRN192 microRNA, human; C531201:MIRN451 microRNA, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; C541915:Mirn129 microRNA, human
keywords: Dysregulation; Epilepsy; Glioma; Seizure; miR-128