title: Heat shock protein 90 is essential for replication of porcine circovirus type 2 in PK-15 cells.
abstract: Porcine circovirus type 2 (PCV2) is recognized as the causative agent of porcine circovirus-associated disease (PCVAD). However, the mechanism of PCV2 replication has not been understood completely. Heat shock protein 90 (Hsp90) plays an important role in viral genome replication, viral genes expression, and viral particle packaging. In this study, we firstly found that inhibition of Hsp90 by pretreatment of host cells with 17-AAG, a specific inhibitor of Hsp90, or blocking Hsp90α/Hsp90β with siRNA, resulted in significantly reduced viral replication in PK-15 cells. But inhibition of Hsp90 by 17-AAG did not affect PCV2 entry into the host cells. Meanwhile, over-expression of Hsp90α/Hsp90β enhanced PCV2 genome replication and virion production. In addition, Hsp90β was enriched in the nuclear zone in the cells infected with PCV2. But it did not interact with the viral Cap/Rep proteins. It suggested that Hsp90 is required for PCV2 production in PK-15 cells culture. It should be helpful for further evaluating the mechanism of replication and pathogenesis of PCV2 and developing novel antiviral therapies.
authors.affiliation: Department of Comparative Medicine, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Comparative Medicine, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, PR China.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Comparative Medicine, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Comparative Medicine, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, PR China.
authors.forename: Jinwei
authors.initials: J
authors.lastname: You
authors.affiliation: Department of Comparative Medicine, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Dong
authors.affiliation: Department of Comparative Medicine, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, PR China. Electronic address: yunshif@163.com.
authors.forename: Shifeng
authors.initials: S
authors.lastname: Yun
authors.affiliation: Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: jiangp@njau.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D016227:Benzoquinones / Q000494:pharmacology; D002460:Cell Line; D018173:Circoviridae Infections / Q000662:veterinary / Q000821:virology*; D017925:Circovirus / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D016679:Genome, Viral; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D047029:Lactams, Macrocyclic / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics; D013552:Swine; D014771:Virion / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D014779:Virus Replication
chemical_list: D016227:Benzoquinones; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D034741:RNA, Small Interfering; C112765:tanespimycin
keywords: Antiviral; Hsp90; Porcine circovirus type 2; Replication