title: Vaterite coatings on electrospun polymeric fibers for biomedical applications.
abstract: The process of porous calcium carbonate (CaCO3) covering on electrospun poly(ε-caprolactone) (PCL) fibers is described in this study. Uniform CaCO3coatings, composed of vaterite microparticles and its aggregates, were formed on PCL fibers by mineral precipitation from solution under ultrasonic treatment. The porous structure of CaCO3in vaterite polymorphic form is useful for loading of various substances (drugs and nanoparticles), and this property makes vaterite an appropriate material for design of drug delivery systems. Such mineralization was implemented to attain therapeutic and/or biological activity of tissue engineering scaffolds based on electrospun PCL, by means of CaCO3coatings. Various structures and polymorphs of CaCO3coatings were obtained by variation of growth conditions (time of fiber incubation in work solution, ultrasonic treatment of this system). Coating homogeneity, CaCO3polymorphic form, morphology, and CaCO3mass can be controlled by number of successive stages of fibrous material treatment. Cytotoxicity tests showed that PCL fibers mineralized with CaCO3did not release substances toxic for cells. SEM images of PCL/CaCO3scaffolds cultured with cells demonstrate that scaffolds supported cell adhesion and spreading. The presented results show the new technique of controlled PCL scaffold mineralization with vaterite, and an opportunity of using PCL/CaCO3as scaffolds for tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 94-103, 2017.
authors.affiliation: Remote Controlled Theranostic Systems Lab, Educational Research Institute of Nanostructures and Biosystem, Saratov State University, Astrakhanskaya, 83, Saratov, 410026, Russia.
authors.forename: Maria S
authors.initials: MS
authors.lastname: Savelyeva
authors.affiliation: Remote Controlled Theranostic Systems Lab, Educational Research Institute of Nanostructures and Biosystem, Saratov State University, Astrakhanskaya, 83, Saratov, 410026, Russia.
authors.forename: Anatoly A
authors.initials: AA
authors.lastname: Abalymov
authors.affiliation: Remote Controlled Theranostic Systems Lab, Educational Research Institute of Nanostructures and Biosystem, Saratov State University, Astrakhanskaya, 83, Saratov, 410026, Russia.
authors.forename: German P
authors.initials: GP
authors.lastname: Lyubun
authors.affiliation: Remote Controlled Theranostic Systems Lab, Educational Research Institute of Nanostructures and Biosystem, Saratov State University, Astrakhanskaya, 83, Saratov, 410026, Russia.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Vidyasheva
authors.affiliation: Remote Controlled Theranostic Systems Lab, Educational Research Institute of Nanostructures and Biosystem, Saratov State University, Astrakhanskaya, 83, Saratov, 410026, Russia.
authors.forename: Alexey M
authors.initials: AM
authors.lastname: Yashchenok
authors.affiliation: Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, 9000, Belgium.
authors.forename: Timothy E L
authors.initials: TE
authors.lastname: Douglas
authors.affiliation: Remote Controlled Theranostic Systems Lab, Educational Research Institute of Nanostructures and Biosystem, Saratov State University, Astrakhanskaya, 83, Saratov, 410026, Russia.|RASA Center in Tomsk, Tomsk Polytechnic University, 634050, Tomsk, Lenin Avenue, 30, Tomsk, 634050, Russia.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Gorin
authors.affiliation: Remote Controlled Theranostic Systems Lab, Educational Research Institute of Nanostructures and Biosystem, Saratov State University, Astrakhanskaya, 83, Saratov, 410026, Russia.|Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, 9000, Belgium.|A.V. Shubnikov Institute of Crystallography Russian Academy of Science, Leninskiy prospect 59, Moscow, 119333, Russia.
authors.forename: Bogdan V
authors.identifier: 0000-0002-6481-3700
authors.initials: BV
authors.lastname: Parakhonskiy
mesh_terms: D002119:Calcium Carbonate / Q000737:chemistry*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D002119:Calcium Carbonate
keywords: calcium carbonate; composite fibers; electrospun scaffolds; functional coating; tissue engineering