title: miRNA-101 supports the osteogenic differentiation in human dental follicle cells.
abstract: OBJECTIVE
  Human dental follicle cells (DFCs) are genuine precursor cells of cementoblasts and alveolar bone osteoblasts. MicroRNAs (miRNAs) represent a class of non-coding endogenous RNAs that silence gene expression post-transcriptionally. miRNA101 actively regulates the osteogenic differentiation of periodontal ligament cells. Therefore the aim of this study was to investigate the role of miRNA101 during the osteogenic differentiation in DFCs.
  
  
  METHODS
  DFCs were isolated, cultivated and osteogenic differentiated in differentiation medium. Total RNA including miRNAs was isolated and the expression of miRNA101 was examined by real-time RT-PCRs. The expression of miRNA101 was induced by miRNA101-mimic transfection and the gene expression of osteogenic transcription factors was obtained by real-time RT-PCRs. Moreover the induction of the osteogenic differentiation was evaluated by the activity of alkaline phosphatase.
  
  
  RESULTS
  miRNA101 was regulated in DFCs during the osteogenic differentiation. After miRNA101-mimic transfection the alkaline phosphatase was increased and the gene expression of typical osteogenic transcription factors such as SP7 (osterix) was up-regulated.
  
  
  CONCLUSIONS
  Our results suggest that miRNA101 sustains the osteogenic differentiation of DFCs.
authors.affiliation: Dept. of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Regensburg, Germany. Electronic address: christoph.klingelhoeffer@ukr.de.
authors.forename: Christoph
authors.initials: C
authors.lastname: Klingelhöffer
authors.affiliation: Dept. of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Regensburg, Germany.
authors.forename: Consuela
authors.initials: C
authors.lastname: Codrin
authors.affiliation: Dept. of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Regensburg, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Ettl
authors.affiliation: Dept. of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Regensburg, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Reichert
authors.affiliation: Dept. of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Regensburg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Morsczeck
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D003795:Dental Sac / Q000166:cytology*; D015870:Gene Expression; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D010012:Osteogenesis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; D000076182:Sp7 Transcription Factor; C478163:SP7 protein, human; D014157:Transcription Factors; D000469:Alkaline Phosphatase
keywords: Human dental follicle cell; Osteogenic differentiation; miRNA101; microRNAs