title: Mammalian Eps15 homology domain 1 promotes metastasis in non-small cell lung cancer by inducing epithelial-mesenchymal transition.
abstract: The identification of the earliest molecular events responsible for the metastatic dissemination of non-small cell lung cancer (NSCLC) remains critical for early detection, prevention, and treatment interventions. In this study, we hypothesized that Mammalian Eps15 homology domain 1 (EHD1) might be responsible for the metastatic behavior of cells in NSCLC. We demonstrated that upregulation of EHD1 is associated with lymph nodes metastasis and unfavorable survival in patients with NSCLC. EHD1 knockdown inhibited the invasion and migration of human NSCLC cells, and overexpression of EHD1 increased the metastatic potential of lung cancer cells. Using the Affymetrix Human Gene 1.0 ST platform, microarray analysis revealed that an association between EHD1 and epithelial-mesenchymal transition (EMT), supported by downregulation of mesenchymal markers and upregulation of epithelial markers following knockdown of EHD1 in cell lines. Moreover, overexpression of EHD1 induced the EMT and increased the metastatic potential of lung cancer cells in vitro and in vivo. These results provide a model to illustrate the relationship between EHD1 expression and lung cancer metastasis, opening up new avenues for the prognosis and therapy of lung cancer.
authors.affiliation: The Fourth Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.|The Sixth Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Meng
authors.affiliation: The Fourth Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xing
authors.affiliation: The Sixth Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Ren
authors.affiliation: The Fourth Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Pathophysiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Xi
authors.affiliation: Department of Pathophysiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: The Fourth Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: The Fourth Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Li
authors.affiliation: The Fourth Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Lichun
authors.initials: L
authors.lastname: Sun
authors.affiliation: The Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin 150081, China.
authors.forename: Dianjun
authors.initials: D
authors.lastname: Sun
authors.affiliation: The Fourth Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150040, China.
authors.forename: Li
authors.initials: L
authors.lastname: Cai
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D046228:Microarray Analysis; D058990:Molecular Targeted Therapy; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D016019:Survival Analysis; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C120700:EHD1 protein, human; D034741:RNA, Small Interfering; D033921:Vesicular Transport Proteins
keywords: EHD1; epithelial-mesenchymal transition; metastasis; non-small cell lung cancer