title: Kinetic analysis of IgM monoclonal antibodies for determination of dengue sample concentration using SPR technique.
abstract: Surface plasmon resonance (SPR) sensing is recently emerging as a valuable technique for measuring the binding constants, association and dissociation rate constants, and stoichimetry for a binding interaction kinetics in a number of emerging biological areas. This technique can be applied to the study of immune system diseases in order to contribute to improved understanding and evaluation of binding parameters for a variety of interactions between antigens and antibodies biochemically and clinically. Since the binding constants determination of an anti-protein dengue antibody (Ab) to a protein dengue antigen (Ag) is mostly complicated, the SPR technique aids a determination of binding parameters directly for a variety of particular dengue Ag_Ab interactions in the real-time. The study highlights the doctrine of real-time dengue Ag_Ab interaction kinetics as well as to determine the binding parameters that is performed with SPR technique. In addition, this article presents a precise prediction as a reference curve for determination of dengue sample concentration.
authors.affiliation: a Institute of Intelligent Manufacturing and Information Engineering , School of Mechanical Engineering, Shanghai Jiao Tong University , Shanghai , P. R. China.|b Integrated Lightwave Research Group, Department of Electrical Engineering , Faculty of Engineering, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Peyman
authors.initials: P
authors.lastname: Jahanshahi
authors.affiliation: a Institute of Intelligent Manufacturing and Information Engineering , School of Mechanical Engineering, Shanghai Jiao Tong University , Shanghai , P. R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
authors.affiliation: a Institute of Intelligent Manufacturing and Information Engineering , School of Mechanical Engineering, Shanghai Jiao Tong University , Shanghai , P. R. China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jie
authors.affiliation: b Integrated Lightwave Research Group, Department of Electrical Engineering , Faculty of Engineering, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Ghomeishi
authors.affiliation: c Department of Medical Microbiology, Department of Microbiology, Faculty of Medicine , University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Shamala Devi
authors.initials: SD
authors.lastname: Sekaran
authors.affiliation: b Integrated Lightwave Research Group, Department of Electrical Engineering , Faculty of Engineering, University of Malaya , Kuala Lumpur , Malaysia.
authors.forename: Faisal Rafiq
authors.initials: FR
authors.lastname: Mahamd Adikan
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D000937:Antigen-Antibody Reactions / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003716:Dengue Virus / Q000276:immunology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology*; D007700:Kinetics; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M
keywords: biosensor; dengue virus; kinetic analysis; surface plasmon resonance