title: Characterization of a manganese peroxidase from white-rot fungus Trametes sp.48424 with strong ability of degrading different types of dyes and polycyclic aromatic hydrocarbons.
abstract: Manganese peroxidase, MnP-Tra-48424, was purified and characterized from the white-rot fungus Trametes sp.48424. MnP-Tra-48424 was strongly resistant to metal ions such as Ni2+, Li+, Ca2+, K+, Mn2+. MnP-Tra-48424 was also resistant to organic solvents such as propanediol, glycerol, and glycol. MnP-Tra-48424 decolorized dyes (indigo, anthraquinone, azo and triphenylmethane) and degraded different polycyclic aromatic hydrocarbons (PAHs). Indigo Carmine, Remazol Brilliant Blue R, Remazol Brilliant Violet 5R and Methyl Green were efficiently decolorized by MnP-Tra-48424. MnP-Tra-48424 also decolorized Indigo Carmine and Methyl Green combined with metal ions and organic solvents. The decolorization capability of MnP-Tra-48424 was not inhibited by selected metal ions and organic solvents. A combination of MnP-Tra-48424 and Lac-Tra-48424 improved the decolorization rate. In addition to dyes, MnP-Tra-48424 was effective at degrading individual PAHs (fluorene, fluoranthene, pyrene, phenanthrene, anthracene) and also PAHs in mixtures.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Feng
authors.initials: F
authors.lastname: He
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Qin
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China; School of Life Science, Central China Normal University, Wuhan, 430079, China. Electronic address: yangy@mail.ccnu.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
mesh_terms: D004396:Coloring Agents / Q000378:metabolism*; D042845:Laccase / Q000302:isolation & purification / Q000378:metabolism; D010544:Peroxidases / Q000302:isolation & purification / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D055454:Trametes / Q000201:enzymology*; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D004396:Coloring Agents; D011084:Polycyclic Aromatic Hydrocarbons; D015262:Xenobiotics; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase
keywords: Decolorization; Laccase; Manganese peroxidase; Polycyclic aromatic hydrocarbon; White-rot fungi