title: Nontargeted Modification-Specific Metabolomics Investigation of Glycosylated Secondary Metabolites in Tea (Camellia sinensis L.) Based on Liquid Chromatography-High-Resolution Mass Spectrometry.
abstract: Glycosylation on small molecular metabolites modulates a series of biological events in plants. However, a large number of glycosides have not been discovered and investigated using -omics approaches. Here, a general strategy named "nontargeted modification-specific metabolomics" was applied to map the glycosylation of metabolites. The key aspect of this method is to adopt in-source collision-induced dissociation to dissociate the glycosylated metabolite, causing a characteristic neutral loss pattern, which acts as an indicator for the glycosylation identification. In an exemplary application in green teas, 120 glucosylated/galactosylated, 38 rhamnosylated, 21 rutinosylated, and 23 primeverosylated metabolites were detected simultaneously. Among them, 61 glycosylated metabolites were putatively identified according to current tea metabolite databases. Thanks to the annotations of glycosyl moieties in advance, the method aids metabolite identifications. An additional 40 novel glycosylated metabolites were tentatively elucidated. This work provides a feasible strategy to discover and identify novel glycosylated metabolites in plants.
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences , 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Dai
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences , 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Tan
authors.affiliation: Agilent Technologies (China) Limited , No. 3 Wangjing North Road, Chaoyang District, Beijing 100102, People's Republic of China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Lu
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences , 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China.
authors.forename: Dongchao
authors.initials: D
authors.lastname: Xie
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences , 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China.
authors.forename: Pengliang
authors.initials: P
authors.lastname: Li
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences , 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China.
authors.forename: Haipeng
authors.initials: H
authors.lastname: Lv
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences , 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences , 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences , 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences , 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China.
authors.forename: Qunhua
authors.initials: Q
authors.lastname: Peng
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences , 9 Meiling South Road, Hangzhou, Zhejiang 310008, People's Republic of China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Lin
mesh_terms: D028241:Camellia sinensis / Q000737:chemistry* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006031:Glycosylation; D013058:Mass Spectrometry; D055432:Metabolomics; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism; D064210:Secondary Metabolism; D013662:Tea / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D013662:Tea
keywords: LC-MS; glycosylation; metabolomics; secondary metabolites; tea