title: Genetic diversity based on MIRU-VNTR profile of isolates of Mycobacterium bovis from Mexican cattle.
abstract: Bovine tuberculosis (bTB) is a disease caused by Mycobacterium bovis (M. bovis), which affects cattle, animal species and humans. To determinate the genetic structure of strains of M. bovis in mexican cattle, 467 isolates obtained from 2009 to 2010 from different regions of Mexico with known spoligotype were included in the study. The isolates were genotyped by interspersed repeated mycobacterial units-variable number tandem repeats (MIRU-VNTR) obtaining 13 MIRU-VNTR groups. When combining MIRU-VNTR patterns with its spolygotypes, the Hunter genetic discrimination index (HGDI), we obtained 421 genetic patterns distributed in 17 groups. The HGDI for the total loci was 0.99. The locus that presented the higher HGDI was 2461 (0.857), while the locus with the lowest HGDI was 2686 (0.239). When we analyzed our results, using just 6 or 8 MIRU-VNTR we obtained an discriminatory power of 0.8499 and 0.8875 respectively indicating lower HGDI than 12 MIRU-VNTR locus.
authors.affiliation: Maestría en Salud y Producción Animal Sustentable, Universidad Autónoma de Querétaro, Querétaro, Mexico. Electronic address: alexnavavargas@hotmail.com.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Nava Vargas
authors.affiliation: Facultad de Ciencias Naturales-Universidad Autónoma de Querétaro, Querétaro, Mexico. Electronic address: miliansf@yahoo.es.
authors.forename: Feliciano
authors.initials: F
authors.lastname: Milián Suazo
authors.affiliation: Facultad de Ciencias Naturales-Universidad Autónoma de Querétaro, Querétaro, Mexico. Electronic address: gcanto99@prodigy.net.mx.
authors.forename: Germinal Jorge
authors.initials: GJ
authors.lastname: Cantó Alarcón
authors.affiliation: Facultad de Ciencias Naturales-Universidad Autónoma de Querétaro, Querétaro, Mexico. Electronic address: yez.rubio@hotmail.com.
authors.forename: Yezenia
authors.initials: Y
authors.lastname: Rubio Venegas
authors.affiliation: Maestría en Salud y Producción Animal Sustentable, Universidad Autónoma de Querétaro, Querétaro, Mexico. Electronic address: caxixi_katzin@hotmail.com.
authors.forename: Roberto
authors.initials: R
authors.lastname: Guerrero Solorio
authors.affiliation: CENID-FyMA-INIFAP, Querétaro, Mexico. Electronic address: rohe577@hotmail.com.
authors.forename: Elba
authors.initials: E
authors.lastname: Rodríguez Hernández
authors.affiliation: CUCS, Departamento de Clínicas Médicas, Universidad de Guadalajara, Jalisco, Mexico. Electronic address: sorrento80@hotmail.com.
authors.forename: Oscar
authors.initials: O
authors.lastname: Pizano Martìnez
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology*; D004269:DNA, Bacterial; D014644:Genetic Variation; D006801:Humans; D008800:Mexico; D018598:Minisatellite Repeats; D009163:Mycobacterium bovis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny
chemical_list: D004269:DNA, Bacterial
keywords: Bovine tuberculosis; Genotyping; MIRU-VNTR; Molecular epidemiology; Mycobacterium bovis; Variable number tandem repeats