title: Phytochemical Profile of Erythrina variegata by Using High-Performance Liquid Chromatography and Gas Chromatography-Mass Spectroscopy Analyses.
abstract: Natural products derived from plant sources have been utilized to treat patients with numerous diseases. The phytochemical constituents present in ethanolic leaf extract of Erythrina variegata (ELEV) were identified by using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectroscopy (GC-MS) analyses. Shade dried leaves were powdered and extracted with ethanol for analyses through HPLC to identify selected flavonoids and through GC-MS to identify other molecules. The HPLC analysis of ELEV showed the presence of gallic and caffeic acids as the major components at concentrations of 2.0 ppm and 0.1 ppm, respectively, as well as other components. GC-MS analysis revealed the presence of 3-eicosyne; 3,7,11,15-tetramethyl-2-hexadecen-1-ol; butanoic acid, 3-methyl-3,7-dimethyl-6-octenyl ester; phytol; 1,2-benzenedicarboxylic acid, diundecyl ester; 1-octanol, 2-butyl-; squalene; and 2H-pyran, 2-(7-heptadecynyloxy) tetrahydro-derivative. Because pharmacopuncture is a new evolving natural mode that uses herbal extracts for treating patients with various ailments with minimum pain and maximum effect, the results of this study are particularly important and show that ELEV possesses a wide range of phytochemical constituents, as indicated above, as effective active principle molecules that can be used individually or in combination to treat patients with various diseases.
authors.affiliation: Department of Biochemistry, School of Biosciences, Periyar University, Salem, Tamil Nadu, India. Electronic address: suriyaveda@yahoo.co.in.
authors.forename: Suriyavathana
authors.initials: S
authors.lastname: Muthukrishnan
authors.affiliation: Department of Biochemistry, School of Biosciences, Periyar University, Salem, Tamil Nadu, India.
authors.forename: Subha
authors.initials: S
authors.lastname: Palanisamy
authors.affiliation: Department of Applied Biology, Adama Science and Technology University, Adama, Ethiopia.
authors.forename: Senthilkumar
authors.initials: S
authors.lastname: Subramanian
authors.affiliation: Department of Biochemistry, School of Biosciences, Periyar University, Salem, Tamil Nadu, India.
authors.forename: Sumathi
authors.initials: S
authors.lastname: Selvaraj
authors.affiliation: Department of Biochemistry, School of Biosciences, Periyar University, Salem, Tamil Nadu, India.
authors.forename: Kavitha Rani
authors.initials: KR
authors.lastname: Mari
authors.affiliation: Department of Biochemistry, School of Biosciences, Periyar University, Salem, Tamil Nadu, India.
authors.forename: Ramalingam
authors.initials: R
authors.lastname: Kuppulingam
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004895:Erythrina / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D064209:Phytochemicals / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D064209:Phytochemicals; D010936:Plant Extracts
keywords: Erythrina variegata; GC-MS; HPLC; flavonoids; phytochemicals