title: [Expression of Btk and NFκB in Acute Lymphoblastic Leukemia Cells and Its Significance].
abstract: OBJECTIVE
  To evaluate the role of Btk and NFκB in the incidence, development, prognosis and therapeutic efficacy for acute lymphoblastic leukemia(ALL) through detecting their expression in leukemia cells.
  
  
  METHODS
  Bone marrow samples from 51 ALL patients were collected, and the mononuclear cells were separated by Ficoll density gradient centrifugation. The expressions of Btk and NFκB at RNA and protein levels were detected by RT-PCR and Western blot respectively.
  
  
  RESULTS
  (1)At protein level, Btk and NFκB were expressed in all newly diagnosed 51 ALL patients, among them 38 patients had higher expression level of Btk, 34 patients had high NFκB expression level. The expression of Btk and NFκB was higher in the cells from newly diagnosed ALL patients than that in the cells from patients in CR(P<0.05), and the expression of Btk and NFκB was higher in relapsed ALL patients. (2)The expression of Btk and NFκB in the ALL patients was followed-up higher expression of Btk: among the 38 newly diagnosed B-ALL cases, 27 experienced CR (71%) and 12 of which achieved CR after one course chemotherapy (one course CR) (31%). Moreover, 16 out of the 27 CR patients relapsed after a short period (less than 6 months) (59%). On the contrary, among the 13 patients with low Btk expression, 11 achieved CR (84.6%) after one course and 1 relapsed (8 months after CR) (7.6%). A similar pattern of NFκB expression was observed.
  
  
  CONCLUSIONS
  Btk and NFκ B may play an important role in the incidence and progression of ALL, possibly serving as the potential therapeutic targets of ALL and the indexes for prognosis.
authors.affiliation: Department of Hematology, Huai'an First People's Hospital Affiliaed to Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Shan-Dong
authors.initials: SD
authors.lastname: Tao
authors.affiliation: Department of Hematology, Huai'an First People's Hospital Affiliaed to Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Chun-Ling
authors.initials: CL
authors.lastname: Wang
authors.affiliation: Department of Hematology, Huai'an First People's Hospital Affiliaed to Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Hematology, Huai'an First People's Hospital Affiliaed to Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Hematology, Huai'an First People's Hospital Affiliaed to Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Li-Tao
authors.initials: LT
authors.lastname: Zhou
authors.affiliation: Department of Hematology, Huai'an First People's Hospital Affiliaed to Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Huai'an First People's Hospital Affiliaed to Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Zheng-Mei
authors.initials: ZM
authors.lastname: He
authors.affiliation: Department of Hematology, Huai'an First People's Hospital Affiliaed to Nanjing Medical University, Huai'an 223300, Jiangsu Province, China. E-mail: yuliangha1965@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
mesh_terms: D000077329:Agammaglobulinaemia Tyrosine Kinase; D006801:Humans; D016328:NF-kappa B; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma; D011379:Prognosis; D011505:Protein-Tyrosine Kinases
chemical_list: D016328:NF-kappa B; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C000625949:BTK protein, human