title: Pharmacology of a traditional Chinese herb, Shuangren-Anshen capsule, in a hemorrhage mouse model by using an orthogonal array design.
abstract: Shuangren-Anshen capsule (SAC) is a traditional Chinese herb that was improved in our laboratory. An orthogonal experiment [L9(3)(4)] was used to optimize the extraction conditions. In vivo, a hemorrhage mouse model was established and the hemoglobin contents of normal control, model control, and treated mice were measured. Additionally, the sedative and hypnotic effects of SACs were assessed based on pharmacological parameters such as changes in locomotive activity, forelimb raising, sleep latency, sleep duration, and number of mice that fell asleep. Brain tissue was sectioned and stained to detect changes in cell morphology by microscopy. The optimum extraction was achieved with 3 cycles of decoction for 120 min each with a 10-fold volume of water added. In the model control group, hemoglobin content significantly decreased and pharmacological parameters increased (P < 0.01) relative to that in the normal control group. Compared to the model control group, the group treated with 0.9 g/kg SAC showed significant (P < 0.05) increase or decrease in hemoglobin content and all pharmacological parameters except sleep duration. The groups treated with 1.8 or 3.6 g/kg SAC and the positive control group also showed significant alterations in hemoglobin content and pharmacological parameters (P < 0.05). In addition, SAC exhibited a protective effect on the morphological structures of the damaged nerve cells in the mouse model. Thus, an optimal extraction process was successfully identified. The pharmacological data also suggests that the drug can improve sleep quality. SAC treatment was shown to cause changes in hemoglobin content and cell morphology in a mouse model.
authors.affiliation: School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China.
authors.forename: X X
authors.initials: XX
authors.lastname: Liu
authors.affiliation: School of Production and Utilization of Food Science, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Agudamu
authors.affiliation: School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wei
authors.affiliation: The Second Hospital of Shanxi Medical University, Taiyuan, China.
authors.forename: X
authors.initials: X
authors.lastname: Song
authors.affiliation: School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China liyunlanrr@163.com.
authors.forename: Y L
authors.initials: YL
authors.lastname: Li
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006470:Hemorrhage / Q000097:blood / Q000188:drug therapy*; D006993:Hypnotics and Sedatives / Q000494:pharmacology; D008297:Male; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D011897:Random Allocation; D007319:Sleep Initiation and Maintenance Disorders / Q000097:blood / Q000473:pathology
chemical_list: D004365:Drugs, Chinese Herbal; D006454:Hemoglobins; D006993:Hypnotics and Sedatives