title: miRNAs in mtDNA-less cell mitochondria.
abstract: The novel regulation mechanism in mtDNA-less cells was investigated. Very low mtDNA copy in mtDNA-less 206 ρ° cells was identified. But no 13 mitochondria-specific proteins were translated in 206 ρ° cells. Their mitochondrial respiration complexes V, III and II were 86.5, 29.4 and 49.6% of 143B cells, respectively. Complexes I and IV completely lack in 206 ρ° cells. Non-mitochondrial respiration to generate ATP in 206 ρ° cells was discovered. The expression levels of some mitochondrial RNAs including 12S rRNA, COX1, COX2, COX3, ND4 and ND5 were low. However, ND1, ND3 and Cyto b were not expressed in 206 ρ° cells. Unequal transcription of mitochondrial RNAs indicated the post-transcriptional cleavage and processing mechanisms in the regulation of mitochondrial gene expression in 206 ρ° cells. MicroRNAs (miRNAs) may modulate these mitochondrial RNA expression in these cells. RNA-induced silencing complex indeed within 206 ρ° cell mitochondria indicated miRNAs in 206 ρ° cell mitochondria. miRNA profile in mtDNA-less 206 ρ° cells was studied by next-generation sequencing of small RNAs. Several mitochondria-enriched miRNAs such as miR-181c-5p and miR-146a-5p were identified in 206 ρ° cell mitochondria. miR-181c-5p and miR-146a-5p had 23 and 19 potential targets on mitochondrial RNAs respectively, and these two miRNAs had multiple targets on mitochondria-associated messenger RNAs encoded by nuclear genes. These data provided the first direct evidence that miRNAs were imported into mitochondria and regulated mitochondrial RNA expressions.
authors.affiliation: Division of Human Genetics, Cincinnati Children's Hospital Medical Center , 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
authors.forename: N
authors.initials: N
authors.lastname: Dasgupta
authors.affiliation: Division of Human Genetics, Cincinnati Children's Hospital Medical Center , 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Division of Human Genetics, Cincinnati Children's Hospital Medical Center , 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Division of Pathology, Cincinnati Children's Hospital Medical Center , 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
authors.forename: G
authors.initials: G
authors.lastname: Ciraolo
authors.affiliation: Maxillofacial Pathology and Radiology Department, Ohio State University College of Dentistry , 304 W. 12th, Columbus, OH 43210, USA.
authors.forename: D
authors.initials: D
authors.lastname: Wang
authors.affiliation: Division of Human Genetics, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45229 USA.
authors.forename: R
authors.initials: R
authors.lastname: Li