title: Quantitative Real-Time PCR Analysis of Gene Transcripts of Mosquito Follicles.
abstract: Real-time (quantitative) PCR, or QPCR, has become an indispensible tool for characterizing gene expression. Depending on the experimental design, researchers can use either the relative or absolute (standard curve) method to quantify transcript abundance. Characterizing the expression of genes in mosquito ovaries will require use of the standard curve method of quantification. Here, I describe reagents and equipment necessary to run standard curve QPCR. I also provide details on the construction of the standard linear curve and calculations required to determine transcript abundance.
authors.affiliation: Biology Program, University of South Florida Sarasota-Manatee, 8350 N. Tamiami Trail, Sarasota, FL, 34243, USA. atelang@usf.edu.
authors.forename: Aparna
authors.initials: A
authors.lastname: Telang
mesh_terms: D000818:Animals; D009033:Culicidae / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D009866:Oogenesis / Q000235:genetics; D006080:Ovarian Follicle / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D059467:Transcriptome
keywords: Gene expression; Mosquito follicles; Mosquito ovary; Quantitative PCR; RNA; Real-time PCR; Standard curve PCR; Transcript abundance