title: Establishment and characterization of a clear cell carcinoma cell line, designated NOCC, derived from human ovary.
abstract: A cell line, designated NOCC, was established from the ascites of a patient with clear cell adenocarcinoma of the ovary. The cell line has been grown without interruption and continuously propagated by serial passaging (more than 76 times) over 7 years. The cells are spherical to polygonal-shaped, display neoplastic, and pleomorphic features, and grow in a jigsaw puzzle-like pattern while forming monolayers without contact inhibition. The cells proliferate rapidly, but are easily floated as a cell sheet. The population doubling time is about 29 h. The number of chromosomes ranges from 60 to 83. The modal number of chromosomes is 70-74 at the 30th passage. NOCC cells secreted 750.5 ng/ml of VEGF over 3 days of culture. Hypoxia inducible factor-1α (HIF-1α) is a primary regulator of VEGF under hypoxic conditions. NOCC cells were not sensitive to the anticancer drugs BEV, DOX, GEM, ETP, CDDP, or TXT. The graft of NOCC cells to a scid mouse displayed similar histological aspects to the original tumor. Both the NOCC cells and the graft of the NOCC cells gave a positive PAS reaction.
authors.affiliation: Department of NDU Life Sciences, School of Life Dentistry, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan. a.ohyama@tky.ndu.ac.jp.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohyama
authors.affiliation: Department of NDU Life Sciences, School of Life Dentistry, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Toyomura
authors.affiliation: Division of Fine Morphology, Core Research Facilities, The Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105-0082, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Tachibana
authors.affiliation: Department of Obstetrics and Gynecology, Jikei Daisan Hospital, 4-11-1 Izumihoncho, Komae-shi, Tokyo, 201-8601, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Isonishi
authors.affiliation: Department of NDU Life Sciences, School of Life Dentistry, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan.|Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Niigata, The Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-1500, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Department of NDU Life Sciences, School of Life Dentistry, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikawa
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002875:Chromosomes; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000502:physiology; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A
keywords: HIF-1α; NOCC cell line; Ovarian clear cell carcinoma; Transplantation; VEGF