title: In vivoLineage-tracing Studies in a Cancer Stem Cell Population in Neuroblastoma.
abstract: Tumors are comprised of heterogeneous subpopulations that may exhibit differing capacity for differentiation, self-renewal, and tumorigenicity.In vivolineage-tracing studies are a powerful tool for defining the role of tumor subpopulations in tumor growth and as targets for therapeutic agents. This protocol describes using a neuroblastoma cancer cell line transduced with two different fluorescent proteins (GFP and td Tomato) to track the specific contributions of cells expressing the GCSF receptor (CD114+) or not (CD114-) on tumor growthin vivo.
authors.affiliation: Department of Surgery, Baylor College of Medicine, Houston, USA.
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Hsu
authors.affiliation: Department of Pediatrics, Baylor College of Medicine, Houston, USA.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Shohet
authors.affiliation: Department of Surgery, Baylor College of Medicine, Houston, USA; Department of Pediatrics, Baylor College of Medicine, Houston, USA.
authors.forename: Eugene S
authors.initials: ES
authors.lastname: Kim