title: PcToll3 was involved in anti-Vibrio response by regulating the expression of antimicrobial peptides in red swamp crayfish, Procambarus clarkii.
abstract: Tolls and Toll-like receptors (TLRs) play an important role in host immune defenses by regulating the expression of antimicrobial peptides (AMPs) and cytokines, but the functional differences of crustacean Tolls from Drosophila Tolls or Mammal TLRs are largely unknown. A novel Toll receptor, named PcToll3, was identified from red swamp crayfish, Procambarus clarkii. It was widely expressed in all detected tissues, and its transcript in hemocytes was up-regulated at 12 h after Vibrio parahemolyticus (Vibrio) injection or at 24 h post white spot syndrome virus (WSSV) challenge. After knockdown of PcToll3, the activity of bacterial clearance was inhibited, and the expression levels of AMPs including Crustin1 (Cru1), Anti-lippopolysaccharide factor 1 (ALF1), and Lysozymes1 (Lys1), which could be up-regulated by Vibrio, were all affected. Meanwhile, PcToll3 silencing influenced the expression of myeloid differentiation factor 88 (PcMyd88), tumor necrosis factor-associated factor 6 (PcTRAF6), and PcDorsal, which were the counterparts of Drosophila Toll signaling pathway. Interestingly, PcToll3 silencing inhibited translocation of PcDorsal from cytoplasm to nucleus. Furthermore, the knockdown of PcDorsal also impaired the expression of AMPs after Vibrio challenge. Hence, we concluded that, besides participating in antiviral immunity, PcToll3 might also regulate the expression of Cru1 and Lys1 to participate in anti-Vibrio immune responses by promoting PcDorsal translocation into nucleus.
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Jiang-Feng
authors.initials: JF
authors.lastname: Lan
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Wei
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Wang
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Yun-Jia
authors.initials: YJ
authors.lastname: Dai
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Jia-Gang
authors.initials: JG
authors.lastname: Tu
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zhao
authors.affiliation: East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, 200090, China.
authors.forename: Xin-Cang
authors.initials: XC
authors.lastname: Li
authors.affiliation: College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Qin
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. Electronic address: chennan@mail.hzau.edu.cn.
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China. Electronic address: linli@mail.hzau.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003400:Astacoidea / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D017422:Sequence Analysis, DNA; D051193:Toll-Like Receptors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014733:Vibrio / Q000502:physiology; D046848:White spot syndrome virus 1 / Q000502:physiology
chemical_list: D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D051193:Toll-Like Receptors
keywords: Antimicrobial peptides; PcDorsal; PcToll3; Red swamp crayfish