title: Metabolic profile and pharmacokinetics of polyphyllin I, an anticancer candidate, in rats by UPLC-QTOF-MS/MS and LC-TQ-MS/MS.
abstract: Polyphyllin I (PPI), a natural steroidal saponin originating from rihzome of Paris polyphylla, is a potential anticancer candidate. Previous pharmacokinetics study showed that the oral bioavailability of PPI was very low, which suggested that certain amount of PPI might be metabolized in vivo. However, to date, information regarding the final metabolic fates of PPI is very limited. In this study, metabolites of PPI and their pharmacokinetics in rats were investigated using UPLC-QTOF-MS/MS and LC-TQ-MS/MS. A total of seven putative metabolites, including six phase I and one phase II metabolites, were detected and identified with three exact structures by comparison with authentic standards for the first time. Oxidation, deglycosylation and glucuronidation were found to be the major metabolic processes of the compound in rats. The pharmacokinetics of prosapogenin A, trillin and diosgenin, three deglycosylation metabolites of PPI with definite anticancer effects, were further studied, which suggested that the metabolites underwent a prolonged absorption and slower elimination after intragastric administration of PPI at the dose of 500 mg/kg. This study provides valuable and new information on the metabolic fate of PPI, which will be helpful in further understanding its mechanism of action.
authors.affiliation: Linyi City Yishui Central Hospital, Yishui, People's Republic of China.
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, People's Republic of China.|Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, People's Republic of China.|Kathmandu University, Kathmandu, Nepal.
authors.forename: Shailendra
authors.initials: S
authors.lastname: Shakya
authors.affiliation: BenQ Medical Center, Nanjing Medical University, Nanjing, People's Republic of China.
authors.forename: Jun-Wen
authors.initials: JW
authors.lastname: Wu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D008297:Male; D055442:Metabolome / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000097:blood / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D012503:Saponins; C556217:polyphyllin I; C026473:prosapogenin; D004144:Diosgenin
keywords: LC-TQ-MS/MS; UPLC-QTOF-MS/MS; metabolite; pharmacokinetics; polyphyllin I