title: Synergic Effect of Borneol and Ligustrazine on the Neuroprotection in Global Cerebral Ischemia/Reperfusion Injury: A Region-Specificity Study.
abstract: The cooperation of ligustrazine (LI) and borneol was proved to be much better than each of them in treating cerebral ischemia. However, the mechanism of their synergic therapy is unclear till now. Moreover, whether their cooperation brought different degrees of protection among different brain regions was also unclear. In the present study, the effects of LI, borneol, and their mixture were observed in global cerebral ischemia-reperfusion (GCIR) injury by detecting microcirculation, expressions of caspase-3 and p53, levels of IL-1β, IL-6, and TNF-α, and contents of SOD, GSH-Px, and MDA in cortex, hippocampus, hypothalamus, and striatum, respectively. Furthermore, Nissl bodies were scored also. Monotherapy of LI or borneol showed obvious improvements in the four regions, specially in cortex and hippocampus. Interestingly, the cooperation of LI and borneol brought some new improvements, specially in hypothalamus and striatum. Thus, the synergic effect of the two drugs showed region-specificity in GCIR injury except the expressions of caspase-3 and p53.
authors.affiliation: Jiangsu Engineering Laboratory for Research and Industrialization of Empirical Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Bin
authors.identifier: 0000-0003-1797-6395
authors.initials: B
authors.lastname: Yu
authors.affiliation: School of Food Science, Nanjing Xiaozhuang University, Nanjing 211117, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ruan
authors.affiliation: Department of Encephalopathy, Nanjing Hospital of Traditional Chinese Medicine, Nanjing 210001, China.
authors.forename: Zhen-Nian
authors.initials: ZN
authors.lastname: Zhang
authors.affiliation: Jiangsu Engineering Laboratory for Research and Industrialization of Empirical Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Hai-Bo
authors.identifier: 0000-0003-0850-3377
authors.initials: HB
authors.lastname: Cheng
authors.affiliation: The Key Laboratory of Optimal Utilization of Natural Medicinal Resources, Guizhou Medical University, Huaxi College Station, Guian New District, Guiyang 550025, China.
authors.forename: Xiang-Chun
authors.identifier: 0000-0002-4333-9106
authors.initials: XC
authors.lastname: Shen