title: Aspergillus fumigatus components distinguish IgE but not IgG4 profiles between fungal sensitization and allergic broncho-pulmonary aspergillosis.
abstract: Aspergillus fumigatus is the causative agent of allergic broncho-pulmonary aspergillosis. Prompt and accurate diagnosis may be difficult to achieve with current clinical and laboratory scores, which do not include immune responses to recombinant A. fumigatus allergens. We measured specific immunoglobulin E and G4 directed to recombinant A. fumigatus allergens in 55 cystic fibrosis patients without allergic broncho-pulmonary aspergillosis but sensitized to A. fumigatus and in nine patients with allergic broncho-pulmonary aspergillosis (two with cystic fibrosis and seven with asthma). IgG4 responses to recombinant A. fumigatus allergens were detected in all patients, but neither prevalence nor levels were different between the two patient groups. On the other hand, both prevalence and levels of IgE responses to Asp f 3, Asp f 4, and Asp f 6 helped distinguish allergic broncho-pulmonary aspergillosis from A. fumigatus sensitization with good negative and positive predictive values.
authors.affiliation: Laboratoire d'Immunologie, Hôpital de La Conception, APHM Assistance Publique Hôpitaux de Marseille, Marseille, France. jvitte@ap-hm.frjvitte@ap-hm.fr.|Faculté de Médecine de Marseille, Aix-Marseille University, Marseille, France. jvitte@ap-hm.frjvitte@ap-hm.fr.
authors.forename: J
authors.initials: J
authors.lastname: Vitte
authors.affiliation: Laboratoire d'Immunologie, Hôpital de La Conception, APHM Assistance Publique Hôpitaux de Marseille, Marseille, France.
authors.forename: T
authors.initials: T
authors.lastname: Romain
authors.affiliation: Faculté de Médecine de Marseille, Aix-Marseille University, Marseille, France.|Centre de Ressources et de Compétences en Mucoviscidose, Hôpital Timone Enfants, APHM Assistance Publique Hôpitaux de Marseille, Marseille, France.
authors.forename: A
authors.initials: A
authors.lastname: Carsin
authors.affiliation: Service de Pneumologie, Hôpital Nord, APHM Assistance Publique Hôpitaux de Marseille, Marseille, France.
authors.forename: M
authors.initials: M
authors.lastname: Gouitaa
authors.affiliation: Centre de Ressources et de Compétences en Mucoviscidose, Hôpital Timone Enfants, APHM Assistance Publique Hôpitaux de Marseille, Marseille, France.
authors.forename: N
authors.initials: N
authors.lastname: Stremler-Le Bel
authors.affiliation: Centre de Ressources et de Compétences en Mucoviscidose, Hôpital Timone Enfants, APHM Assistance Publique Hôpitaux de Marseille, Marseille, France.
authors.forename: M
authors.initials: M
authors.lastname: Baravalle-Einaudi
authors.affiliation: Laboratoire d'Immunologie, Hôpital de La Conception, APHM Assistance Publique Hôpitaux de Marseille, Marseille, France.
authors.forename: I
authors.initials: I
authors.lastname: Cleach
authors.affiliation: Faculté de Médecine de Marseille, Aix-Marseille University, Marseille, France.|Centre de Ressources et de Compétences en Mucoviscidose, Hôpital Nord, APHM Assistance Publique Hôpitaux de Marseille, Marseille, France.
authors.forename: M
authors.initials: M
authors.lastname: Reynaud-Gaubert
authors.affiliation: Faculté de Médecine de Marseille, Aix-Marseille University, Marseille, France.|Centre de Ressources et de Compétences en Mucoviscidose, Hôpital Timone Enfants, APHM Assistance Publique Hôpitaux de Marseille, Marseille, France.
authors.forename: J-C
authors.initials: JC
authors.lastname: Dubus
authors.affiliation: Laboratoire d'Immunologie, Hôpital de La Conception, APHM Assistance Publique Hôpitaux de Marseille, Marseille, France.|Faculté de Médecine de Marseille, Aix-Marseille University, Marseille, France.
authors.forename: J-L
authors.initials: JL
authors.lastname: Mège
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology*; D001229:Aspergillosis, Allergic Bronchopulmonary / Q000453:epidemiology / Q000276:immunology* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000276:immunology*; D002648:Child; D003550:Cystic Fibrosis / Q000150:complications; D006801:Humans; D007114:Immunization; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D016036:Seroepidemiologic Studies; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000946:Antigens, Fungal; D007074:Immunoglobulin G; D007073:Immunoglobulin E
keywords: 
  Aspergillus fumigatus
  ; IgE; IgG4; allergic broncho-pulmonary aspergillosis; cystic fibrosis