title: [Differentiation of K562 Cells Induced by Pulsatilla Saponin A into Erythroid Lineage].
abstract: OBJECTIVE
  To explore the differentiation-inducing potentiality of Pulsatilla saponin A on K562 cells.
  
  
  METHODS
  Pulsatilla saponin A of different concentrations was used to treat K562 cells; the benzidine staining and the hemoglobinometry were applied to measure the change of hemoglobin content; the flow cytometry (FCM) was used to detect the expression of CD71 and GPA on K562 cells.
  
  
  RESULTS
  K562 cells treated with 4 µg/ml pulsatilla saponin A differentiated into the erythroid lineage. With the treatment of pulsatilla saponin A, the hemoglobin content in K562 cells increased significantly; CD71 and GPA expression on the K562 cell surface were up-regulated.
  
  
  CONCLUSIONS
  Pulsatilla saponin A can induce K562 cells to differentiate into erythroid lineage.
authors.affiliation: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China.
authors.forename: Tong
authors.initials: T
authors.lastname: Wang
authors.affiliation: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China.
authors.forename: Ri
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China. E-mail：qixf-sz@hotmail.com.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Qi
authors.affiliation: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Bai
authors.affiliation: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China.
authors.forename: Fang
authors.initials: F
authors.lastname: Gong
authors.affiliation: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China.
authors.forename: Zi-Xing
authors.initials: ZX
authors.lastname: Chen
authors.affiliation: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China.
authors.forename: Jian-Nong
authors.initials: JN
authors.lastname: Cen
authors.affiliation: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China.
authors.forename: Ming-Qing
authors.initials: MQ
authors.lastname: Zhu
authors.affiliation: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China.
authors.forename: Lan
authors.initials: L
authors.lastname: Dai
mesh_terms: D000970:Antineoplastic Agents; D002454:Cell Differentiation; D019070:Cell Lineage; D041905:Erythroid Cells; D006801:Humans; D020014:K562 Cells; D012503:Saponins
chemical_list: D000970:Antineoplastic Agents; D012503:Saponins; C000588664:Pulsatilla saponin A