title: Elevated eukaryotic elongation factor 2 expression is involved in proliferation and invasion of lung squamous cell carcinoma.
abstract: Eukaryotic elongation factor 2 (EF2), is a critical enzyme solely responsible for catalyzing the translocation of the elongated peptidyl-tRNA from the A to P sites of the ribosome during the process of protein synthesis. EF2 is found to be highly expressed in a variety of malignant tumors and is correlated with cancer cell progression and recurrence. The present study was designed to uncover the function of EF2 on lung squamous cell carcinoma (LSCC) cancer cell growth and progression. Our results from clinical tissue studies showed that EF2 protein was significantly overexpressed in LSCC tissues, compared with the adjacent normal lung tissues, which was confirmed by western blotting and tissue microarray. Forced expression of EF2 resulted in the enhancement of lung squamous carcinoma NCI-H520 cells growth through promotion of G2/M progression in cell cycle, activating Akt and Cdc2/Cyclin B1. In nude mice cancer xenograft model, overexpression of EF2 significantly facilitated cell proliferation in vivo. Furthermore, forced expression of EF2 in the cells increased the capabilities of migration and invasion by changing the expressions of EMT-related proteins and genes. These results provided novel insights into the role of EF2 in tumorigenesis and progression in LSCC. EF2-targeted therapy could become a good strategy for the clinical treatment of LSCC.
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, Dalian, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Chest Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, Dalian, China.
authors.forename: LiHong
authors.initials: L
authors.lastname: Hao
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, Dalian, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Institute of Cancer Stem Cell, Dalian Medical University Cancer Center, Liaoning, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Du
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, Dalian, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, Dalian, China.
authors.forename: LiYuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, Dalian, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, Dalian, China.
authors.forename: LinLin
authors.initials: L
authors.lastname: Gong
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, Dalian, China.
authors.forename: XinMing
authors.initials: X
authors.lastname: Chi
authors.affiliation: Institute of Cancer Stem Cell, Dalian Medical University Cancer Center, Liaoning, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, Dalian, China.|Liaoning Key Laboratory of Proteomics, Dalian Medical University, Liaoning, China.
authors.forename: ShuJuan
authors.initials: S
authors.lastname: Shao
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018450:Disease Progression; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local / Q000235:genetics; D009368:Neoplasm Transplantation; D040901:Proteomics / Q000379:methods; D046888:Tissue Array Analysis
chemical_list: D015852:Eukaryotic Initiation Factor-2
keywords: eukaryotic elongation factor 2; invasion; lung squamous cell carcinoma; proliferation; proteomics