title: A Novel Insecticidal Peptide SLP1 Produced by Streptomyces laindensis H008 against Lipaphis erysimi.
abstract: Aphids are major insect pests for crops, causing damage by direct feeding and transmission of plant diseases. This paper was completed to discover and characterize a novel insecticidal metabolite against aphids from soil actinobacteria. An insecticidal activity assay was used to screen 180 bacterial strains from soil samples against mustard aphid, Lipaphis erysimi. The bacterial strain H008 showed the strongest activity, and it was identified by the phylogenetic analysis of the 16S rRNA gene and physiological traits as a novel species of genus Streptomyces (named S. laindensis H008). With the bioassay-guided method, the insecticidal extract from S. laindensis H008 was subjected to chromatographic separations. Finally, a novel insecticidal peptide was purified from Streptomyces laindensis H008 against L. erysimi, and it was determined to be S-E-P-A-Q-I-V-I-V-D-G-V-D-Y-W by TOF-MS and amino acid analysis.
authors.affiliation: Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, Heilongjiang, China. xulijian@hlju.edu.cn.|College of Agricultural Resource and Environment, Heilongjiang University, Harbin 150080, China. xulijian@hlju.edu.cn.
authors.forename: Lijian
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, Heilongjiang, China. 2141174@s.hlju.edu.cn.
authors.forename: Kangkang
authors.initials: K
authors.lastname: Liang
authors.affiliation: Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, Heilongjiang, China. 2141169@s.hlju.edu.cn.
authors.forename: Bensha
authors.initials: B
authors.lastname: Duan
authors.affiliation: Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, Heilongjiang, China. ymengdi@yahoo.com.
authors.forename: Mengdi
authors.initials: M
authors.lastname: Yu
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, China. mengwei@nefu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Meng
authors.affiliation: College of Agricultural Resource and Environment, Heilongjiang University, Harbin 150080, China. qgwang1970@163.com.
authors.forename: Qinggui
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, Heilongjiang, China. 2004077@hlju.edu.cn.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000187:drug effects; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D007306:Insecticides / Q000302:isolation & purification* / Q000494:pharmacology*; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007306:Insecticides; D010455:Peptides; D012336:RNA, Ribosomal, 16S; C000617418:SLP1 pentadecapeptide, Streptomyces laindensis
keywords: Lipaphis erysimi; Rhopalosiphum pseudobrassicae; Streptomyces; actinobacteria; aphid; insecticidal; peptide; pesticide