title: The functional interplay of Rab11, FIP3 and Rho proteins on the endosomal recycling pathway controls cell shape and symmetry.
abstract: Several families of small GTPases regulate a variety of fundamental cellular processes, encompassing growth factor signal transduction, vesicular trafficking and control of the cytoskeleton. Frequently, their action is hierarchical and complementary, but much of the detail of their functional interactions remains to be clarified. It is well established that Rab family members regulate a variety of intracellular vesicle trafficking pathways. Moreover, Rho family GTPases are pivotal for the control of the actin and microtubule cytoskeleton. However, the interplay between these 2 types of GTPases has been rarely reported. We discuss here our recent findings showing that Rab11, a key regulator of endosomal recycling, and Rac1, a central actin cytoskeleton regulator involved in lamellipodium formation and cell migration, interplay on endosomes through the Rab11 effector FIP3. In the context of the rapidly reactive T lymphocytes, Rab11-Rac1 endosomal functional interplay is important to control cell shape changes and cell symmetry during lymphocyte spreading and immunological synapse formation and ultimately modulate T cell activation.
authors.affiliation: a Institut Pasteur, Department of Immunology , Lymphocyte Cell Biology Unit , Paris , France.|b INSERM U1221 , Paris , France.|c Institut Cochin, INSERM U1016, CNRS, UMR8104, Université Paris Descartes, Sorbonne Paris Cité , Paris , France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bouchet
authors.affiliation: d Molecular Cell Biology Laboratory, School of Biochemistry and Cell Biology, Biosciences Institute, University College , Cork , Ireland.
authors.forename: Mary W
authors.initials: MW
authors.lastname: McCaffrey
authors.affiliation: e Università Vita e Salute San Raffaele , Milano , Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Graziani
authors.affiliation: a Institut Pasteur, Department of Immunology , Lymphocyte Cell Biology Unit , Paris , France.|b INSERM U1221 , Paris , France.
authors.forename: Andrés
authors.initials: A
authors.lastname: Alcover
mesh_terms: D048430:Cell Shape; D011992:Endosomes / Q000378:metabolism*; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D020741:rho GTP-Binding Proteins / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D051550:I-kappa B Kinase; D020691:rab GTP-Binding Proteins; D020741:rho GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins
keywords: FIP3; immunological synapse; Rab11; Rac; T cell activation; cell shape; cell symmetry; recycling endosomes