title: Structure determination of transient transcription complexes.
abstract: The determination of detailed 3D structures of large and transient multicomponent complexes remains challenging. Here I describe the approaches that were used and developed by our laboratory to achieve structure solution of eukaryotic transcription complexes. I hope this collection serves as a resource for structural biologists seeking solutions for difficult structure determination projects.
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany pcramer@mpibpc.mpg.de.
authors.forename: Patrick
authors.initials: P
authors.lastname: Cramer
mesh_terms: D020285:Cryoelectron Microscopy; D003460:Crystallization; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D012319:RNA Polymerase II / Q000737:chemistry* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D035581:Transcription Factor TFIIB / Q000737:chemistry* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D046912:Multiprotein Complexes; D029701:Saccharomyces cerevisiae Proteins; D035581:Transcription Factor TFIIB; D012319:RNA Polymerase II
keywords: X-ray crystallography; cryo-electron microscopy; eukaryotic RNA polymerase complex; integrated structural biology; multiprotein assembly