title: Metabolic changes in Euphorbia palusrtis latex after fungal infection.
abstract: The variations of metabolic profile of the latex of wild-growing Euphorbia palustris was carried out using multivariate analysis of1H NMR spectral data. One population was infected with fungi Fusarium sporotrichioides, Fusarium proliferatum and Alternaria alternata, while the other consisted of healthy plant species. The non-polar metabolites of latex extracts such as benzoyl ingenol-laurate, amyrin decadienoate esters, cis-1,4-polyisoprene, and 24-methylenecycloartanol were identified using1H and 2D NMR spectra. Principal component analysis of1H NMR data provided a clear discrimination between the latex of infected and healthy plants. Minimum inhibitory concentration and minimum fungicidal concentration values of the latex extracts of healthy and infected plants were determined. The latex of infected plants was found to contain higher levels of benzoyl ingenol-laurate and 24-methylenecycloartanol, of which concentrations were strongly correlated with the antifungal activities of the latex.
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Gordana
authors.initials: G
authors.lastname: Krstić
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Boban
authors.initials: B
authors.lastname: Anđelković
authors.affiliation: Natural Products Laboratory, Institute of Biology, Leiden University, 2300 RA, Leiden, The Netherlands.
authors.forename: Young Hae
authors.initials: YH
authors.lastname: Choi
authors.affiliation: Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Vlatka
authors.initials: V
authors.lastname: Vajs
authors.affiliation: Institute for Medicinal Plants Research ''Dr. Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Stević
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Vele
authors.initials: V
authors.lastname: Tešević
authors.affiliation: Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia. Electronic address: dgodjev@chem.bg.ac.rs.
authors.forename: Dejan
authors.initials: D
authors.lastname: Gođevac
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D004224:Diterpenes; D028481:Euphorbia / Q000378:metabolism*; D005658:Fungi / Q000187:drug effects; D005670:Fusarium / Q000187:drug effects; D045782:Hemiterpenes / Q000378:metabolism; D007840:Latex / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009181:Mycoses; D019906:Nuclear Magnetic Resonance, Biomolecular; D010840:Phytosterols / Q000032:analysis
chemical_list: D000935:Antifungal Agents; D004224:Diterpenes; D045782:Hemiterpenes; D007840:Latex; D010840:Phytosterols; C455100:1,4-polyisoprene; C008125:ingenol
keywords: Antifungal activity; Euphorbiaceae; Euporbia palustris; Multivariate data analysis; NMR spectroscopy