title: Morphoagronomic characterization and genetic diversity of a common bean RIL mapping population derived from the cross Rudá x AND 277.
abstract: Recombinant inbred lines (RILs) are a valuable resource for building genetic linkage maps. The presence of genetic variability in the RILs is essential for detecting associations between molecular markers and loci controlling agronomic traits of interest. The main goal of this study was to quantify the genetic diversity of a common bean RIL population derived from a cross between Rudá (Mesoamerican gene pool) and AND 277 (Andean gene pool). This population was developed by the single seed descent method from 500 F2 plants until the F10 generation. Seven quantitative traits were evaluated in the field in 393 RILs, the parental lines, and five control cultivars. The plants were grown using a randomized block design with additional controls and three replicates. Significant differences were observed among the RILs for all evaluated traits (P < 0.01). A comparison of the RILs and parental lines showed significant differences (P < 0.01) for the number of days to flowering (DFL) and to harvest (DH), productivity (PROD) and mass of 100 beans (M100); however, there were no significant differences for plant architecture, degree of seed flatness, or seed shape. These results indicate the occurrence of additive x additive epistatic interactions for DFL, DH, PROD, and M100. The 393 RILs were shown to fall into 10 clusters using Tocher's method. This RIL population clearly contained genetic variability for the evaluated traits, and this variability will be crucial for future studies involving genetic mapping and quantitative trait locus identification and analysis.
authors.affiliation: Programa de Pós-Graduação em Genética e Melhoramento, Universidade Federal de Viçosa, Viçosa, MG, Brasil leocalvino@yahoo.com.br.
authors.forename: L C
authors.initials: LC
authors.lastname: Silva
authors.affiliation: Programa de Pós-Graduação em Genética e Melhoramento, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: R O
authors.initials: RO
authors.lastname: Batista
authors.affiliation: Programa de Pós-Graduação em Genética e Melhoramento, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: R S R
authors.initials: RS
authors.lastname: Anjos
authors.affiliation: Departamento de Fitotecnia, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: M H
authors.initials: MH
authors.lastname: Souza
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: P C S
authors.initials: PC
authors.lastname: Carneiro
authors.affiliation: Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, Brasil.
authors.forename: T L P O
authors.initials: TL
authors.lastname: Souza
authors.affiliation: Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF, Brasil.
authors.forename: E G
authors.initials: EG
authors.lastname: Barros
authors.affiliation: Departamento de Fitotecnia, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: J E S
authors.initials: JE
authors.lastname: Carneiro
mesh_terms: D004843:Epistasis, Genetic; D017343:Genes, Plant; D056426:Genetic Loci; D014644:Genetic Variation; D027805:Phaseolus / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D000069600:Plant Breeding; D040641:Quantitative Trait Loci