title: Mapping QTLs for drought tolerance in an F2:3 population from an inter-specific cross between Gossypium tomentosum and Gossypium hirsutum.
abstract: Cotton is one of the most important natural fiber crops in the world. Its growth and yield is greatly limited by drought. A quantitative trait locus (QTL) analysis was therefore conducted to investigate the genetic basis of drought tolerance in cotton (Gossypium spp) using 188 F2:3 lines developed from an inter-specific cross between a wild cotton species, G. tomentosum, and an upland cotton, G. hirsutum (CRI-12). A genetic map was constructed using 1295 simple sequence repeat markers, which amplified 1342 loci, distributed on 26 chromosomes, covering 3328.24 cM. A field experiment was conducted in two consecutive years (2014 and 2015) and 11 morphological and physiological traits were recorded under water-limited (W1)/well-watered (W2) regimes at three growth stages (bud, flowering, and full boll). The traits measured included chlorophyll content, plant height, leaf area, leaf number, leaf fresh weight, leaf dry weight, boll weight, number of bolls per plant, and the number of fruiting branches. Sixty-seven and 35 QTLs were found under the W1 and W2 conditions, respectively. Of these, the majority exhibited partial dominance or over-dominance genetic effects for increasing the trait values. Four consistent QTLs were found under the W1 treatment on chromosomes 5, 8, 9, and 16, whereas no consistent QTL was found in W2. Thirteen QTL clusters were also identified on nine chromosomes (2, 3, 5, 6, 9, 14, 15, 16, and 21). These results will help to elucidate the genetic basis of drought tolerance in cotton.
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, China.|Economic Crops Research Institute of Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, China.|School of Biological and Physical Sciences, Jaramogi Oginga Odinga University of Science and Technology, Bondo, Kenya.
authors.forename: G
authors.initials: G
authors.lastname: Oluoch
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, China.
authors.forename: M K
authors.initials: MK
authors.lastname: Riaz Khan
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, China.
authors.forename: X X
authors.initials: XX
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, China.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, China.
authors.forename: C Y
authors.initials: CY
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Economic Crops Research Institute of Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, China.
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, China wkbcri@163.com.
authors.forename: K B
authors.initials: KB
authors.lastname: Wang
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D055864:Droughts; D005819:Genetic Markers; D003368:Gossypium / Q000235:genetics*; D018895:Microsatellite Repeats; D040641:Quantitative Trait Loci; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D005819:Genetic Markers