title: Prediction of Protection against Asian Enterovirus 71 Outbreak Strains by Cross-neutralizing Capacity of Serum from Dutch Donors, The Netherlands.
abstract: Outbreaks of human enterovirus 71 (EV-71) in Asia are related to high illness and death rates among children. To gain insight into the potential threat for the population of Europe, we determined the neutralizing activity in intravenous immunoglobulin (IVIg) batches and individual serum samples from donors in the Netherlands against EV-71 strains isolated in Europe and in Asia. All IVIg batches and 41%, 79%, and 65% of serum samples from children ≤5 years of age, women of childbearing age, and HIV-positive men, respectively, showed high neutralizing activity against a Dutch C1 strain, confirming widespread circulation of EV-71 in the Netherlands. Asian B3-4 and C4 strains were efficiently cross-neutralized, predicting possible protection against extensive circulation and associated outbreaks of those types in Europe. However, C2 and C5 strains that had few mutations in the capsid region consistently escaped neutralization, emphasizing the importance of monitoring antigenic diversity among circulating EV-71 strains.
authors.forename: Sabine M G
authors.initials: SM
authors.lastname: van der Sanden
authors.forename: Gerrit
authors.initials: G
authors.lastname: Koen
authors.forename: Hetty
authors.initials: H
authors.lastname: van Eijk
authors.forename: Sylvie M
authors.initials: SM
authors.lastname: Koekkoek
authors.forename: Menno D
authors.initials: MD
authors.lastname: de Jong
authors.forename: Katja C
authors.initials: KC
authors.lastname: Wolthers
mesh_terms: D000328:Adult; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D036022:Capsid Proteins / Q000235:genetics; D002460:Cell Line; D002675:Child, Preschool; D060085:Coinfection; D056738:Cross Protection / Q000276:immunology; D004196:Disease Outbreaks; D029821:Enterovirus A, Human / Q000145:classification / Q000235:genetics / Q000276:immunology*; D004769:Enterovirus Infections / Q000453:epidemiology* / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D005260:Female; D005838:Genotype; D015658:HIV Infections; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000627:therapeutic use; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D009426:Netherlands / Q000453:epidemiology; D009500:Neutralization Tests; D011159:Population Surveillance; D016036:Seroepidemiologic Studies; D010948:Viral Plaque Assay; D055815:Young Adult
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D036022:Capsid Proteins; D016756:Immunoglobulins, Intravenous
keywords: Asian enterovirus 71; Dutch; Europe; Picornaviruses; cross protection; cross-neutralization; enterovirus; human enterovirus 71; intravenous immunoglobulins; seroprevalence; the Netherlands; viruses