title: Probing the Fractal Pattern of Heartbeats in Drosophila Pupae by Visible Optical Recording System.
abstract: Judiciously tuning heart rates is critical for regular cardiovascular function. The fractal pattern of heartbeats - a multiscale regulation in instantaneous fluctuations - is well known for vertebrates. The most primitive heart system of the Drosophila provides a useful model to understand the evolutional origin of such a fractal pattern as well as the alterations of fractal pattern during diseased statuses. We developed a non-invasive visible optical heart rate recording system especially suitable for long-term recording by using principal component analysis (PCA) instead of fluorescence recording system to avoid the confounding effect from intense light irradiation. To deplete intracellular Ca(2+) levels, the expression of sarco-endoplasmic reticulum Ca(2+)-ATPase (SERCA) was tissue-specifically knocked down. The SERCA group shows longer heart beat intervals (Mean ± SD: 1009.7 ± 151.6 ms) as compared to the control group (545.5 ± 45.4 ms, p < 0.001). The multiscale correlation of SERCA group (scaling exponent: 0.77 ± 0.07), on the other hand, is weaker than that of the control Drosophila (scaling exponent: 0.85 ± 0.03) (p = 0.016).
authors.affiliation: Department of Biomedical Sciences and Engineering, National Central University, Taoyuan city, Taiwan.
authors.forename: Chen
authors.initials: C
authors.lastname: Lin
authors.affiliation: Department of Biomedical Sciences and Engineering, National Central University, Taoyuan city, Taiwan.|Institue of Molecular Medicine, National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Yi-Chung
authors.initials: YC
authors.lastname: Chang
authors.affiliation: Institue of Molecular Medicine, National Taiwan University College of Medicine, Taipei, Taiwan.|Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Ya-Chen
authors.initials: YC
authors.lastname: Cheng
authors.affiliation: Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Po-Jung
authors.initials: PJ
authors.lastname: Lai
authors.affiliation: Department of Biomedical Sciences and Engineering, National Central University, Taoyuan city, Taiwan.|Medical Biodynamics Program, Division of Sleep and Circadian Disorders, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.|Department of Electrical Engineering, National Central University, Taoyuan city, Taiwan.
authors.forename: Chien-Hung
authors.initials: CH
authors.lastname: Yeh
authors.affiliation: Department of Biomedical Sciences and Engineering, National Central University, Taoyuan city, Taiwan.
authors.forename: Wan-Hsin
authors.initials: WH
authors.lastname: Hsieh
authors.affiliation: Medical Biodynamics Program, Division of Sleep and Circadian Disorders, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors.forename: Kun
authors.initials: K
authors.lastname: Hu
authors.affiliation: Institue of Molecular Medicine, National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: June-Tai
authors.initials: JT
authors.lastname: Wu
authors.affiliation: Institue of Molecular Medicine, National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Hsiu-Hsiang
authors.initials: HH
authors.lastname: Lee
authors.affiliation: Department of Biomedical Sciences and Engineering, National Central University, Taoyuan city, Taiwan.
authors.forename: Men-Tzung
authors.initials: MT
authors.lastname: Lo
authors.affiliation: Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
authors.forename: Yi-Lwun
authors.initials: YL
authors.lastname: Ho
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D004330:Drosophila / Q000254:growth & development / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D017709:Fractals; D006339:Heart Rate; D007814:Larva / Q000502:physiology; D055096:Optical Devices; D025341:Principal Component Analysis; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism
chemical_list: D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium