title: The levels of 7,8-dihydrodeoxyguanosine (8-oxoG) and 8-oxoguanine DNA glycosylase 1 (OGG1) - A potential diagnostic biomarkers of Alzheimer's disease.
abstract: Evidence indicates that oxidative stress contributes to neuronal cell death in Alzheimer's disease (AD). Increased oxidative DNA damage l, as measured with 8-oxoguanine (8-oxoG), and reduced capacity of proteins responsible for removing of DNA damage, including 8-oxoguanine DNA glycosylase 1 (OGG1), were detected in brains of AD patients. In the present study we assessed peripheral blood biomarkers of oxidative DNA damage, i.e. 8- oxoG and OGG1, in AD diagnosis, by comparing their levels between the patients and the controls. Our study was performed on DNA and serum isolated from peripheral blood taken from 100 AD patients and 110 controls. For 8-oxoG ELISA was employed. The OGG1 level was determined using ELISA and Western blot technique. Levels of 8-oxoG were significantly higher in DNA of AD patients. Both ELISA and Western blot showed decreased levels of OGG1 in serum of AD patients. Our results show that oxidative DNA damage biomarkers detected in peripheral tissue could reflect the changes occurring in the brain of patients with AD. These results also suggest that peripheral blood samples may be useful to measure oxidative stress biomarkers in AD.
authors.affiliation: Department of Internal Disease, Diabetology and Clinical Pharmacology, Medical University of Lodz, Lodz, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sliwinska
authors.affiliation: University of Lodz, Department of Molecular Genetics, Lodz, Poland.
authors.forename: Dominik
authors.initials: D
authors.lastname: Kwiatkowski
authors.affiliation: University of Lodz, Department of Molecular Genetics, Lodz, Poland; Department of Medical Biochemistry, Medical University of Lodz, Lodz, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Czarny
authors.affiliation: University of Lodz, Department of Molecular Genetics, Lodz, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Toma
authors.affiliation: University of Lodz, Department of Molecular Genetics, Lodz, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Wigner
authors.affiliation: Department of Internal Disease, Diabetology and Clinical Pharmacology, Medical University of Lodz, Lodz, Poland.
authors.forename: Jozef
authors.initials: J
authors.lastname: Drzewoski
authors.affiliation: Department of Biomedical Chemistry, Medical University of Lodz, Lodz, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Fabianowska-Majewska
authors.affiliation: Department of Medical Biochemistry, Medical University of Lodz, Lodz, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Szemraj
authors.affiliation: Deakin University IMPACT Strategic Research Centre, Deakin University, School of Medicine, Barwon Health, Geelong, Australia; Department of Psychiatry, Chulalongkorn University, Bangkok, Thailand; Health Sciences Graduate Program, Health Sciences Center, State University of Londrina, Brazil.
authors.forename: Michael
authors.initials: M
authors.lastname: Maes
authors.affiliation: Department of Adult Psychiatry, Medical University of Lodz, Lodz, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Galecki
authors.affiliation: University of Lodz, Department of Molecular Genetics, Lodz, Poland. Electronic address: tomsliw@biol.uni.lodz.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Sliwinski
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000097:blood*; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis; D015153:Blotting, Western; D045647:DNA Glycosylases / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D008297:Male; D012372:ROC Curve
chemical_list: C453560:7,8-dihydro-8-oxoguanine; D015415:Biomarkers; D006147:Guanine; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human
keywords: 7,8-dihydrodeoxyguanosine (8-oxoG); 8-oxoguanine DNA glycosylase 1 (OGG1); Alzheimer's disease; DNA base excision repair; Oxidative DNA damage; Oxidative stress