title: MicroRNA-200b-3p suppresses epithelial-mesenchymal transition and inhibits tumor growth of glioma through down-regulation of ERK5.
abstract: Epithelial-mesenchymal transition (EMT) plays a pivotal role in the development of cancer. Has-miR-200b-3p is generally recognized as one of the fundamental regulators of EMT. In this study, we found that the expression of miR-200b-3p was downregulated in glioma tissues and human glioma cells U87 and U251. Meanwhile, Up-regulating miR-200b-3p enhanced E-cadherin, reduced mesenchymal markers, and decreased cell proliferation, migration, and invasion in vitro. In vivo, the xenograft mouse model also unveiled the suppressive effects of miR-200b-3p on tumor growth. Additionally, The extracellular-regulated protein kinase 5 (ERK5) was confirmed as a direct target gene of miR-200b-3p. The direct suppression of ERK5 expressions by miR-200b-3p was revealed by luciferase reporter assay, quantitative RT-PCR analysis, and western blot. Moreover, we observed an inverse correlation between miR-200b-3p and ERK5 in human glioma tissues. In summary, our ﬁndings demonstrated that miR-200b-3p suppresses glioma tumor growth, invasion, and reverses EMT through downregulated its target ERK5.
authors.affiliation: Department of Neurosurgery, The Second Hospital of Tianjin Medical University, Tianjin, 300211, China. Electronic address: wood2008cn@sina.com.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, The Second Hospital of Tianjin Medical University, Tianjin, 300211, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Cui
authors.affiliation: Department of Neurosurgery, The Second Hospital of Tianjin Medical University, Tianjin, 300211, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, The Second Hospital of Tianjin Medical University, Tianjin, 300211, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D057809:HEK293 Cells; D006801:Humans; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D048054:Mitogen-Activated Protein Kinase 7 / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness
chemical_list: C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D048054:Mitogen-Activated Protein Kinase 7
keywords: EMT; ERK5; Glioma; Has-miR-200b-3p (miR-200b-3p); Invasion