title: Systematic Approach to Optimization of Experimental Conditions in Nonequilibrium Capillary Electrophoresis of Equilibrium Mixtures.
abstract: Nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) is an efficient method for studying intermolecular interactions. Optimization of NECEEM experiments is not a trivial task, due to the complex interrelation between numerous experimental parameters and their combined effects on the accuracy and precision of measurements. Here we present an "algorithmic" approach for NECEEM optimization, which eliminates all of the guesswork out of this process and allows researchers to approach it in a systematic manner. We have fully tested our approach using comprehensive in silico analysis and have showed its utility within a real experimental study. The new approach makes NECEEM more robust, resilient to errors, and easily approachable for researchers with varying experience in CE.
authors.affiliation: Department of Chemistry and Centre for Research on Biomolecular Interactions, York University , Toronto, Ontario M3J 1P3, Canada.
authors.forename: Mirzo
authors.initials: M
authors.lastname: Kanoatov
authors.affiliation: Department of Chemistry and Centre for Research on Biomolecular Interactions, York University , Toronto, Ontario M3J 1P3, Canada.
authors.forename: Sina
authors.initials: S
authors.lastname: Mehrabanfar
authors.affiliation: Department of Chemistry and Centre for Research on Biomolecular Interactions, York University , Toronto, Ontario M3J 1P3, Canada.
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Krylov