title: [Influence of Co-inhibiting mTORC2 and HSP90 on Proliferation Apoptosis of Multiple Myeloma Cells].
abstract: Objective：To explore the influence of co-inhibiting mTORC2 and HSP90 on the proliferation and apoptosis of multiple myeloma(MM) cell line U266.
  
  
  METHODS
  During culture, the human MM cell line U266 were treated with 20 nmol/L of rapamycin, 600 nmol/L 17-AAG, 20 nmol/L of rapamycin + 600 nmol/L 17-AGG and phosphate-buffered saline (PBS), then the growth inhibition rate, morphologic changes, apoptosis rate and the expression of caspase 3 and ATK protein in U266 cells were compared and analyzed.
  
  
  RESULTS
  The rapamycin and 17-AAG both could inhibit the growth of U266 cells, while the inhibitory effect of rapamycin in combination with 17-AAG on growth of U266 cells was significantly higher them that of rapamycin and 17-AAG alone and control (PBS); the apoptosis rate of U266 cells treated with rapamycin, 17-AAG and their combination was higher than that of control PBS groups, and the efficacy of 2 drug conbination was higher than that of control PBS group, and the efficacy of 2 drug combination was superior to single drug. The expression levels of caspase 3 and ATK in U266 cells treated with rapamycin, 17-AAG and their combination were higher and lower than those in control group respectively, and the efficacy of 2 drug combination was superior to signle drug. There were significant difference between them (P<0.05).
  
  
  CONCLUSIONS
  The co-inhibition of mTORC2 and HSP90 can suppress the proliferation and induce the apoptosis of MM cells.
authors.affiliation: School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu Province,China; Department of Hematology, Huaian First Affiliated Hospital of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Kan-Kan
authors.initials: KK
authors.lastname: Chen
authors.affiliation: Department of Hematology, Huaian First Affiliated Hospital of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Hematology, Huaian First Affiliated Hospital of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Zheng-Mei
authors.initials: ZM
authors.lastname: He
authors.affiliation: Department of Hematology, Huaian First Affiliated Hospital of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Li-Tao
authors.initials: LT
authors.lastname: Zhou
authors.affiliation: Department of Hematology, Huaian First Affiliated Hospital of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Li-Juang
authors.initials: LJ
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Huaian First Affiliated Hospital of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Li-Xiao
authors.initials: LX
authors.lastname: Song
authors.affiliation: Department of Hematology, Huaian First Affiliated Hospital of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Bang-He
authors.initials: BH
authors.lastname: Ding
authors.affiliation: Department of Hematology, Huaian First Affiliated Hospital of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Chun-Ling
authors.initials: CL
authors.lastname: Wang
authors.affiliation: Department of Hematology, Huaian First Affiliated Hospital of Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
authors.affiliation: School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu Province,China. E-mail：13701465635@126.com.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis; D016227:Benzoquinones; D053148:Caspase 3; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018841:HSP90 Heat-Shock Proteins; D006801:Humans; D047029:Lactams, Macrocyclic; D000076225:Mechanistic Target of Rapamycin Complex 2; D009101:Multiple Myeloma; D046912:Multiprotein Complexes; D020123:Sirolimus; D058570:TOR Serine-Threonine Kinases
chemical_list: D016227:Benzoquinones; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D046912:Multiprotein Complexes; C112765:tanespimycin; D000076225:Mechanistic Target of Rapamycin Complex 2; D058570:TOR Serine-Threonine Kinases; C505407:CASP3 protein, human; D053148:Caspase 3; D020123:Sirolimus