title: Soybean Golgi SNARE 12 protein interacts with Soybean mosaic virus encoded P3N-PIPO protein.
abstract: Soybean mosaic virus (SMV), a member of the Potyvirus genus, is one of the most prevalent and devastating viral pathogens in soybean-growing regions worldwide. It is generally accepted that symptom development of a viral plant disease results from molecular interactions between the virus and its host plant. P3N-PIPO, as a trans-frame protein consisting of the amino-terminal half of P3 fused to PIPO of the Potyvirus, plays a key role of viral cell-to-cell movement. This study provides evidence that Golgi SNARE 12 (designated as GOS12) protein of soybean interacts with SMV P3N-PIPO via a two-hybrid yeast system by screening a soybean cDNA library. Bimolecular fluorescence complementation (BiFC) assay further confirmed the interaction, which occurred in the cytomembrane of Nicotiana benthamiana cells. We also confirmed that the domain involved in the interaction is PIPO domain of P3N-PIPO by two-hybrid yeast system and BiFC. It is possible that the GOS12 is an essential host factor for PD targeting of P3N-PIPO protein of potyvirus.
authors.affiliation: Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China.
authors.forename: Puwen
authors.initials: P
authors.lastname: Song
authors.affiliation: National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Haijian
authors.initials: H
authors.lastname: Zhi
authors.affiliation: Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China.
authors.forename: Bingyue
authors.initials: B
authors.lastname: Wu
authors.affiliation: Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China. Electronic address: cxy@jaas.ac.cn.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Cui
authors.affiliation: Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China. Electronic address: jaascx@sohu.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
mesh_terms: D005453:Fluorescence; D017800:Potyvirus / Q000378:metabolism*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D030262:Soybean Proteins / Q000737:chemistry / Q000378:metabolism*; D013025:Glycine max / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D030262:Soybean Proteins; D014764:Viral Proteins
keywords: Bimolecular fluorescence complementation; Golgi SNARE 12; Interaction; P3N-PIPO; Soybean mosaic virus; Yeast two hybrid