title: Evaluation of bone matrix gelatin/fibrin glue and chitosan/gelatin composite scaffolds for cartilage tissue engineering.
abstract: This study was designed to evaluate bone matrix gelatin (BMG)/fibrin glue and chitosan/gelatin composite scaffolds for cartilage tissue engineering. Chondrocytes were isolated from costal cartilage of Sprague-Dawley rats and seeded on BMG/fibrin glue or chitosan/gelatin composite scaffolds. After different in vitro culture durations, the scaffolds were subjected to hematoxylin and eosin, Masson's trichrome, and toluidine blue staining, anti-collagen II and anti-aggrecan immunohistochemistry, and scanning electronic microscopy (SEM) analysis. After 2 weeks of culture, chondrocytes were distributed evenly on the surfaces of both scaffolds. Cell numbers and the presence of extracellular matrix components were markedly increased after 8 weeks of culture, and to a greater extent on the chitosan/gelatin scaffold. The BMG/fibrin glue scaffold showed signs of degradation after 8 weeks. Immunofluorescence analysis confirmed higher levels of collagen II and aggrecan using the chitosan/gelatin scaffold. SEM revealed that the majority of cells on the surface of the BMG/fibrin glue scaffold demonstrated a round morphology, while those in the chitosan/gelatin group had a spindle-like shape, with pseudopodia. Chitosan/gelatin scaffolds appear to be superior to BMG/ fibrin glue constructs in supporting chondrocyte attachment, proliferation, and biosynthesis of cartilaginous matrix components.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology, The Affiliated Hospital of Yan'an University, Yan'an, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: J
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhao
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Yang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: D M
authors.initials: DM
authors.lastname: Kong
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Shi
authors.affiliation: Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Li
mesh_terms: D000269:Adhesives / Q000009:adverse effects; D053674:Aggrecans / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001857:Bone Matrix / Q000737:chemistry; D002448:Cell Adhesion; D002478:Cells, Cultured; D048271:Chitosan / Q000009:adverse effects; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020219:Chondrogenesis / Q000187:drug effects; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D005337:Fibrin / Q000009:adverse effects; D005780:Gelatin / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D000269:Adhesives; D053674:Aggrecans; D024043:Collagen Type II; D005780:Gelatin; D005337:Fibrin; D048271:Chitosan