title: MicroRNAs and Drinking: Association between the Pre-miR-27a rs895819 Polymorphism and Alcohol Consumption in a Mediterranean Population.
abstract: Recently, microRNAs (miRNA) have been proposed as regulators in the different processes involved in alcohol intake, and differences have been found in the miRNA expression profile in alcoholics. However, no study has focused on analyzing polymorphisms in genes encoding miRNAs and daily alcohol consumption at the population level. Our aim was to investigate the association between a functional polymorphism in the pre-miR-27a (rs895819 A>G) gene and alcohol consumption in an elderly population. We undertook a cross-sectional study of PREvención con DIeta MEDiterránea (PREDIMED)-Valencia participants (n = 1007, including men and women aged 67 ± 7 years) and measured their alcohol consumption (total and alcoholic beverages) through a validated questionnaire. We found a strong association between the pre-miR-27a polymorphism and total alcohol intake, this being higher in GG subjects (5.2 ± 0.4 in AA, 5.9 ± 0.5 in AG and 9.1 ± 1.8 g/day in GG; padjusted = 0.019). We also found a statistically-significant association of the pre-miR-27a polymorphism with the risk of having a high alcohol intake (>2 drinks/day in men and >1 in women): 5.9% in AA versus 17.5% in GG; padjusted < 0.001. In the sensitivity analysis, this association was homogeneous for sex, obesity and Mediterranean diet adherence. In conclusion, we report for the first time a significant association between a miRNA polymorphism (rs895819) and daily alcohol consumption.
authors.affiliation: Department of Preventive Medicine and Public Health, School of Medicine, University of Valencia, Valencia 46010, Spain. rocio.barragan@uv.es.|CIBER Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid 28029, Spain. rocio.barragan@uv.es.
authors.forename: Rocío
authors.initials: R
authors.lastname: Barragán
authors.affiliation: CIBER Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid 28029, Spain. oscar.coltell@uji.es.|Department of Computer Languages and Systems, School of Technology and Experimental Sciences, Universitat Jaume I, Castellón 12071, Spain. oscar.coltell@uji.es.
authors.forename: Oscar
authors.initials: O
authors.lastname: Coltell
authors.affiliation: Department of Preventive Medicine and Public Health, School of Medicine, University of Valencia, Valencia 46010, Spain. eva.m.asensio@uv.es.|CIBER Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid 28029, Spain. eva.m.asensio@uv.es.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Asensio
authors.affiliation: Department of Preventive Medicine and Public Health, School of Medicine, University of Valencia, Valencia 46010, Spain. francesc.frances@uv.es.|CIBER Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid 28029, Spain. francesc.frances@uv.es.
authors.forename: Francesc
authors.initials: F
authors.lastname: Francés
authors.affiliation: Department of Preventive Medicine and Public Health, School of Medicine, University of Valencia, Valencia 46010, Spain. jose.sorli@uv.es.|CIBER Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid 28029, Spain. jose.sorli@uv.es.
authors.forename: José V
authors.initials: JV
authors.lastname: Sorlí
authors.affiliation: CIBER Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid 28029, Spain. RESTRUCH@clinic.cat.|Department of Internal Medicine, Hospital Clinic, IDIBAPS, Barcelona 08036, Spain. RESTRUCH@clinic.cat.
authors.forename: Ramon
authors.initials: R
authors.lastname: Estruch
authors.affiliation: CIBER Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid 28029, Spain. albert.salas@uab.cat.|Human Nutrition Unit, Biochemistry and Biotechnology Department, IISPV, University Rovira i Virgili, Reus 43003, Spain. albert.salas@uab.cat.
authors.forename: Albert
authors.initials: A
authors.lastname: Salas-Huetos
authors.affiliation: Department of Cardiovascular Epidemiology and Population Genetics, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid 28029, Spain. jose.ordovas@tufts.edu.|IMDEA Alimentación, Madrid 28049, Spain. jose.ordovas@tufts.edu.|Nutrition and Genomics Laboratory, JM-USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA. jose.ordovas@tufts.edu.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Ordovas
authors.affiliation: Department of Preventive Medicine and Public Health, School of Medicine, University of Valencia, Valencia 46010, Spain. dolores.corella@uv.es.|CIBER Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, Madrid 28029, Spain. dolores.corella@uv.es.
authors.forename: Dolores
authors.initials: D
authors.lastname: Corella
mesh_terms: D000368:Aged; D000428:Alcohol Drinking / Q000235:genetics*; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D019083:Mediterranean Region; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: C531388:MIRN27 microRNA, human; D035683:MicroRNAs
keywords: Mediterranean; alcohol; miR27a; microRNAs