title: The association between MMP2 -1306 C > T (rs243865) polymorphism and risk of prostate cancer.
abstract: BACKGROUND
  Prostate cancer is the second most common cancer in men. Matrix metalloproteinase-2 (MMP2) is the most important member of the matrix metalloproteinase family. MMP2 digests the basement membrane and causes changes in the extracellular matrix which in turn facilitate cancer invasion. It, therefore, has a major role in tumor angiogenesis. Previous studies have identified a single-nucleotide polymorphism C/T at position -1306 of MMP2 gene promoter which is a key regulatory factor in cancer progression.
  
  
  OBJECTIVE
  The present study aimed to determine the association between MMP2 polymorphism and the risk of prostate cancer in Iranian men.
  
  
  METHODS
  This case-control study was performed on 50 paraffin-embedded prostate cancer tissue samples and 54 blood samples from healthy men. Genotyping of the samples was performed using high-resolution melting analysis (HRM). Finally, 20 % of the genotypes were confirmed by sequencing.
  
  
  RESULTS
  No significant associations were found between CT and TT genotypes and the risk of prostate cancer. However, there were no significant relationships between the genotypes and the studied factors, e.g., age, pathological stage, and Gleason Score.
  
  
  CONCLUSIONS
  MMP2 -1306 C > T (rs243865) polymorphism was not significantly related with prostate cancer susceptibility in Iranian men.
authors.affiliation: Department of Biology, Faculty of Biological Sciences, Parand Branch, Islamic Azad University, Parand, Iran.
authors.forename: L
authors.initials: L
authors.lastname: Shajarehpoor Salavati
authors.affiliation: Department of Biology, Faculty of Biological Sciences, Parand Branch, Islamic Azad University, Parand, Iran. farzanehtafvizi54@gmail.com.
authors.forename: F
authors.initials: F
authors.lastname: Tafvizi
authors.affiliation: Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: H K
authors.initials: HK
authors.lastname: Manjili
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D018450:Disease Progression; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007492:Iran; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics*; D012306:Risk
chemical_list: D020778:Matrix Metalloproteinase 2
keywords: High-resolution melting (HRM); Matrix metalloproteinase-2 (MMP2); Prostate cancer