title: Fluorescence-Based Strategies to Investigate the Structure and Dynamics of Aptamer-Ligand Complexes.
abstract: In addition to the helical nature of double-stranded DNA and RNA, single-stranded oligonucleotides can arrange themselves into tridimensional structures containing loops, bulges, internal hairpins and many other motifs. This ability has been used for more than two decades to generate oligonucleotide sequences, so-called aptamers, that can recognize certain metabolites with high affinity and specificity. More recently, this library of artificially-generated nucleic acid aptamers has been expanded by the discovery that naturally occurring RNA sequences control bacterial gene expression in response to cellular concentration of a given metabolite. The application of fluorescence methods has been pivotal to characterize in detail the structure and dynamics of these aptamer-ligand complexes in solution. This is mostly due to the intrinsic high sensitivity of fluorescence methods and also to significant improvements in solid-phase synthesis, post-synthetic labeling strategies and optical instrumentation that took place during the last decade. In this work, we provide an overview of the most widely employed fluorescence methods to investigate aptamer structure and function by describing the use of aptamers labeled with a single dye in fluorescence quenching and anisotropy assays. The use of 2-aminopurine as a fluorescent analog of adenine to monitor local changes in structure and fluorescence resonance energy transfer (FRET) to follow long-range conformational changes is also covered in detail. The last part of the review is dedicated to the application of fluorescence techniques based on single-molecule microscopy, a technique that has revolutionized our understanding of nucleic acid structure and dynamics. We finally describe the advantages of monitoring ligand-binding and conformational changes, one molecule at a time, to decipher the complexity of regulatory aptamers and summarize the emerging folding and ligand-binding models arising from the application of these single-molecule FRET microscopy techniques.
authors.affiliation: Laboratory for Biophysics and Biomolecular Dynamics, SUPA School of Physics and Astronomy, University of St. Andrews St Andrews, UK.
authors.forename: Cibran
authors.initials: C
authors.lastname: Perez-Gonzalez
authors.affiliation: RNA Group, Department of Biology, Faculty of Science, Université de Sherbrooke Sherbrooke, QC, Canada.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Lafontaine
authors.affiliation: Laboratory for Biophysics and Biomolecular Dynamics, SUPA School of Physics and Astronomy, University of St. AndrewsSt Andrews, UK; Laboratory for Biophysics and Biomolecular Dynamics, Biomedical Sciences Research Complex, School of Biology, University of St. AndrewsSt. Andrews, UK.
authors.forename: J Carlos
authors.initials: JC
authors.lastname: Penedo
keywords: 2-aminopurine; aptamer dynamics; fluorescence; forster resonance energy transfer (FRET); single-molecule microscopy