title: Quantum dot-loaded monofunctionalized DNA icosahedra for single-particle tracking of endocytic pathways.
abstract: Functionalization of quantum dots (QDs) with a single biomolecular tag using traditional approaches in bulk solution has met with limited success. DNA polyhedra consist of an internal void bounded by a well-defined three-dimensional structured surface. The void can house cargo and the surface can be functionalized with stoichiometric and spatial precision. Here, we show that monofunctionalized QDs can be realized by encapsulating QDs inside DNA icosahedra and functionalizing the DNA shell with an endocytic ligand. We deployed the DNA-encapsulated QDs for real-time imaging of three different endocytic ligands-folic acid, galectin-3 (Gal3) and the Shiga toxin B-subunit (STxB). Single-particle tracking of Gal3- or STxB-functionalized QD-loaded DNA icosahedra allows us to monitor compartmental dynamics along endocytic pathways. These DNA-encapsulated QDs, which bear a unique stoichiometry of endocytic ligands, represent a new class of molecular probes for quantitative imaging of endocytic receptor dynamics.
authors.affiliation: Chemical Biology of Membranes and Therapeutic Delivery unit, Institut Curie, PSL Research University, Institut national de la santé et de la recherche médicale, U 1143, Centre national de la recherche scientifique, Unité mixte de recherche 3666, 26 rue d'Ulm, 75248 Paris Cedex 05, France.|National Centre for Biological Sciences, Tata Institute of Fundamental Research, Gandhi Krishi Vigyan Kendra, Bellary Road, Bangalore 560065, India.
authors.forename: Dhiraj
authors.identifier: 0000-0002-1478-6417
authors.initials: D
authors.lastname: Bhatia
authors.affiliation: Chemical Biology of Membranes and Therapeutic Delivery unit, Institut Curie, PSL Research University, Institut national de la santé et de la recherche médicale, U 1143, Centre national de la recherche scientifique, Unité mixte de recherche 3666, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
authors.forename: Senthil
authors.initials: S
authors.lastname: Arumugam
authors.affiliation: Laboratoire Physique et Etude des Matériaux UMR8213 École Supérieure de Physique et de Chimie Industrielles ParisTech-CNRS - Université Pierre et Marie Curie Sorbonne Universités 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Nasilowski
authors.affiliation: Department of Physics, Center for Condensed Matter Theory, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Joshi
authors.affiliation: Chemical Biology of Membranes and Therapeutic Delivery unit, Institut Curie, PSL Research University, Institut national de la santé et de la recherche médicale, U 1143, Centre national de la recherche scientifique, Unité mixte de recherche 3666, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Wunder
authors.affiliation: Chemical Biology of Membranes and Therapeutic Delivery unit, Institut Curie, PSL Research University, Institut national de la santé et de la recherche médicale, U 1143, Centre national de la recherche scientifique, Unité mixte de recherche 3666, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Chambon
authors.affiliation: National Centre for Biological Sciences, Tata Institute of Fundamental Research, Gandhi Krishi Vigyan Kendra, Bellary Road, Bangalore 560065, India.|Department of Chemistry, The University of Chicago, 929 E, 57th Street, Chicago, Illinois 60637, USA.
authors.forename: Ved
authors.initials: V
authors.lastname: Prakash
authors.affiliation: Nexdot, 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Chloé
authors.initials: C
authors.lastname: Grazon
authors.affiliation: Nexdot, 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Brice
authors.initials: B
authors.lastname: Nadal
authors.affiliation: Department of Physics, Center for Condensed Matter Theory, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Prabal K
authors.initials: PK
authors.lastname: Maiti
authors.affiliation: Chemical Biology of Membranes and Therapeutic Delivery unit, Institut Curie, PSL Research University, Institut national de la santé et de la recherche médicale, U 1143, Centre national de la recherche scientifique, Unité mixte de recherche 3666, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
authors.forename: Ludger
authors.initials: L
authors.lastname: Johannes
authors.affiliation: Laboratoire Physique et Etude des Matériaux UMR8213 École Supérieure de Physique et de Chimie Industrielles ParisTech-CNRS - Université Pierre et Marie Curie Sorbonne Universités 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Dubertret
authors.affiliation: National Centre for Biological Sciences, Tata Institute of Fundamental Research, Gandhi Krishi Vigyan Kendra, Bellary Road, Bangalore 560065, India.|Department of Chemistry, The University of Chicago, 929 E, 57th Street, Chicago, Illinois 60637, USA.|Grossman Institute for Neuroscience, Quantitative Biology and Human Behavior, The University of Chicago, 5812 South Ellis Avenue, Chicago, Illinois 60637, USA.
authors.forename: Yamuna
authors.initials: Y
authors.lastname: Krishnan
mesh_terms: D000818:Animals; D003412:Cricetulus; D004247:DNA / Q000737:chemistry*; D000067493:Dynamic Light Scattering; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005492:Folic Acid / Q000737:chemistry; D037502:Galectin 3 / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D046529:Microscopy, Electron, Transmission; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D045663:Quantum Dots / Q000737:chemistry*; D022603:Shiga Toxins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D037502:Galectin 3; D022603:Shiga Toxins; C077239:stxB toxin; D004247:DNA; D005492:Folic Acid