title: Evaluation of a selected lactic acid bacteria-based probiotic on Salmonella enterica serovar Enteritidis colonization and intestinal permeability in broiler chickens.
abstract: Two experiments were conducted to evaluate the effect of a lactic acid bacteria-based probiotic (FloraMax-B11®) against Salmonella enterica serovar Enteritidis intestinal colonization and intestinal permeability in broiler chickens. Experiment 1 consisted of two independent trials. In each trial, day-old broiler chicks were assigned to one of two groups: control + S. Enteritidis or probiotic + S. Enteritidis. At 72 h post-S. Enteritidis challenge, haematology and caecal content were evaluated for S. Enteritidis colonization. In Experiment 2, day-old broiler chicks were assigned to one of four groups: negative control; probiotic; control + S. Enteritidis; or probiotic + S. Enteritidis. At 72 h post-S. Enteritidis challenge, chickens in all groups were given an oral gavage dose of fluorescein isothiocyanate dextran (FITC-d). In both trials of Experiment 1, a significant reduction (P < 0.05) in colony-forming units/gram of S. Enteritidis in caecal content and a reduction in the incidence of S. Enteritidis enriched caecal samples were observed in probiotic + S. Enteritidis chickens. In addition, significant heterophilia and lymphopaenia were observed in control + S. Enteritidis chickens. In Experiment 2, a decrease in numbers of S. Enteritidis in caeca were observed in probiotic + S. Enteritidis chickens when compared to control + S. Enteritidis. Also, an increase in serum FITC-d concentration was detected in control + S. Enteritidis. These results suggest that early infection with S. Enteritidis can increase intestinal permeability, but the adverse effects can be prevented by the administration of the probiotic tested.
authors.affiliation: a Facultad de Medicina Veterinaria y Zootecnia , Universidad de Colima , Colima , México.
authors.forename: Omar F
authors.initials: OF
authors.lastname: Prado-Rebolledo
authors.affiliation: a Facultad de Medicina Veterinaria y Zootecnia , Universidad de Colima , Colima , México.
authors.forename: Jaime de Jesus
authors.initials: JJ
authors.lastname: Delgado-Machuca
authors.affiliation: a Facultad de Medicina Veterinaria y Zootecnia , Universidad de Colima , Colima , México.
authors.forename: Rafael J
authors.initials: RJ
authors.lastname: Macedo-Barragan
authors.affiliation: b Centro Universitario de Investigación y Desarrollo Agrícola , Universidad de Colima , Colima , México.
authors.forename: Luis J
authors.initials: LJ
authors.lastname: Garcia-Márquez
authors.affiliation: c Departamento de Producción Agrícola y Animal , Universidad Autónoma Metropolitana , México City , México.
authors.forename: Jesus E
authors.initials: JE
authors.lastname: Morales-Barrera
authors.affiliation: d Department of Poultry Science, Center of Excellence for Poultry Science , University of Arkansas , Fayetteville , AR , USA.
authors.forename: Juan D
authors.initials: JD
authors.lastname: Latorre
authors.affiliation: e Departamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y Zootecnia , Universidad Nacional Autónoma de México , México City , México.
authors.forename: Xochitl
authors.initials: X
authors.lastname: Hernandez-Velasco
authors.affiliation: d Department of Poultry Science, Center of Excellence for Poultry Science , University of Arkansas , Fayetteville , AR , USA.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Tellez
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D002432:Cecum / Q000187:drug effects / Q000382:microbiology; D002645:Chickens / Q000382:microbiology*; D007422:Intestines / Q000187:drug effects / Q000382:microbiology; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D010539:Permeability / Q000187:drug effects; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D019936:Probiotics / Q000008:administration & dosage*; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000187:drug effects*
chemical_list: D019344:Lactic Acid
keywords: Broiler; Salmonella Enteritidis; intestinal permeability; probiotic