title: Web based scoring is useful for validation and harmonisation of scoring criteria within RENEB.
abstract: To establish a training data set of digital images and to investigate the scoring criteria and dose assessment of the dicentric assay within the European network of biodosimetry (RENEB), a web based scoring inter-comparison was undertaken by 17 RENEB partners.
  
  
  
  Two sets of 50 high resolution images were uploaded onto the RENEB website. One set included metaphases after a moderate exposure (1.3 Gy) and the other set consisted of metaphases after a high dose exposure (3.5 Gy). The laboratories used their own calibration curves for estimating doses based on observed aberration frequencies.
  
  
  
  The dose estimations and 95% confidence limits were compared to the actual doses and the corresponding z-values were satisfactory for the majority; only the dose estimations from two laboratories were too low or too high. The coefficients of variation were 17.6% for the moderate and 11.2% for the high dose. Metaphases with controversial results could be identified for training purposes.
  
  
  
  Overall, the web based scoring of the two galleries by the 17 laboratories produced very good results. Application of web based scoring for the dicentric assay may therefore be a relevant strategy for an operational biodosimetry assistance network.
authors.affiliation: a Bundesamt fuer Strahlenschutz , Neuherberg , Germany.
authors.forename: Horst
authors.initials: H
authors.lastname: Romm
authors.affiliation: b Public Health England, CRCE , Chilton , Didcot , UK.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Ainsbury
authors.affiliation: c Universitat Autònoma de Barcelona , Barcelona , Spain.
authors.forename: Joan Francesc
authors.initials: JF
authors.lastname: Barquinero
authors.affiliation: c Universitat Autònoma de Barcelona , Barcelona , Spain.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Barrios
authors.affiliation: d Bundeswehr Institute of Radiobiology affiliated to the University of Ulm , Munich , Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Beinke
authors.affiliation: e Institutul National de Sanatate Publica , Bucharest , Romania.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Cucu
authors.affiliation: f Servicio Madrileño de Salud - Hospital General Universitario Gregorio Marañón , Madrid , Spain.
authors.forename: Mercedes Moreno
authors.initials: MM
authors.lastname: Domene
authors.affiliation: g Department of Ecological and Biological Sciences , University of Tuscia , Viterbo , Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Filippi
authors.affiliation: h Centro de Ciêincias e Tecnologias Nucleares, Instituto Superior Técnico, Universidade de Lisboa , Lisboa , Portugal.
authors.forename: Octávia
authors.initials: O
authors.lastname: Monteiro Gil
authors.affiliation: i Institut de Radioprotection et de Sûreté Nucléaire , Fontenay-aux-Roses , France.
authors.forename: Eric
authors.initials: E
authors.lastname: Gregoire
authors.affiliation: j National Centre for Radiobiology and Radiation Protection , Sofia , Bulgaria.
authors.forename: Valeria
authors.initials: V
authors.lastname: Hadjidekova
authors.affiliation: k National Centre for Scientific Research 'Demokritos' , Athens , Greece.
authors.forename: Vasia
authors.initials: V
authors.lastname: Hatzi
authors.affiliation: l Radiation and Nuclear Safety Authority , Helsinki , Finland.
authors.forename: Carita
authors.initials: C
authors.lastname: Lindholm
authors.affiliation: m Commissariat à l´ Énergie Atomique , Paris , France.|n Cell Environment , Paris , France.
authors.forename: Radhia
authors.initials: R
authors.lastname: M Kacher
authors.affiliation: o Hospital Universitario y Politécnico la Fe , Valencia , Spain.
authors.forename: Alegria
authors.initials: A
authors.lastname: Montoro
authors.affiliation: b Public Health England, CRCE , Chilton , Didcot , UK.
authors.forename: Jayne
authors.initials: J
authors.lastname: Moquet
authors.affiliation: e Institutul National de Sanatate Publica , Bucharest , Romania.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Noditi
authors.affiliation: a Bundesamt fuer Strahlenschutz , Neuherberg , Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Oestreicher
authors.affiliation: g Department of Ecological and Biological Sciences , University of Tuscia , Viterbo , Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Palitti
authors.affiliation: k National Centre for Scientific Research 'Demokritos' , Athens , Greece.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Pantelias
authors.affiliation: f Servicio Madrileño de Salud - Hospital General Universitario Gregorio Marañón , Madrid , Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Prieto
authors.affiliation: e Institutul National de Sanatate Publica , Bucharest , Romania.
authors.forename: Irina
authors.initials: I
authors.lastname: Popescu
authors.affiliation: p University Medical Centre Hamburg-Eppendorf , Hamburg , Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Rothkamm
authors.affiliation: o Hospital Universitario y Politécnico la Fe , Valencia , Spain.
authors.forename: Natividad
authors.initials: N
authors.lastname: Sebastià
authors.affiliation: q Institut Chemii i Techniki Jadrowej , Warszawa , Poland.
authors.forename: Sylwester
authors.initials: S
authors.lastname: Sommer
authors.affiliation: k National Centre for Scientific Research 'Demokritos' , Athens , Greece.
authors.forename: Georgia
authors.initials: G
authors.lastname: Terzoudi
authors.affiliation: r Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile , Rome , Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Testa
authors.affiliation: s Stockholm University, Department of Molecular Biosciences, Stockholm , Sweden and Jan Kochanowski University , Kielce , Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wojcik
mesh_terms: D001681:Biological Assay / Q000379:methods*; D020732:Cytogenetic Analysis / Q000379:methods*; D005060:Europe; D006801:Humans; D020407:Internet / Q000458:organization & administration*; D007753:Laboratories / Q000706:statistics & numerical data; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D000069079:Radiation Exposure / Q000032:analysis*; D011834:Radiation Monitoring / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: Web based scoring; biodosimetry network; biological dosimetry; dicentric assay; radiation