title: Icariin attenuated oxidative stress induced-cardiac apoptosis by mitochondria protection and ERK activation.
abstract: Our previous study showed that Icariin (ICA) has anti-cardiac hypertrophy effect in rats with an unknown mechanism. In the present study, we aimed to clarify the cardiac protective effect and mechanism of ICA in vitro. H9C2 cardiac myocytes were incubated with H2O2to build up the oxidative stress injury model. The results showed that pre-treatment of ICA protected cells against the toxicity induced by H2O2. H2O2treatment significantly reduced H2O2-induced apoptosis, evidenced by lower Annexin V/PI stained cells and less PARP and caspase-3/9 activation. Mitochondria membrane potential (MMP) dissipation occurred following the exposure of H2O2, which could be prevented by ICA treatment. Moreover, Ca2+homeostasis was preserved by ICA and ROS generation was significantly suppressed by ICA incubation. Interestingly, ICA treatment increased the phosphorylation of upstream ERK mitogen-activated protein kinase (MAPK) while ERK inhibitor U1026 could reverse the protective effect of ICA. Overall, ICA seems to protect the cardiac cells from oxidative stress injury through ROS scavenge and stimulation of ERK pathway which may explain its effects in vivo.
authors.affiliation: Department of Cardiology, The Third Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Jinling Road, Nanjing 210001, China.
authors.forename: Yao-Hong
authors.initials: YH
authors.lastname: Song
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, Nanjing Jinling Hospital, 305 Zhongshandong Road, Nanjing 210002, China. Electronic address: phdcaihui@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Cai
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, Nanjing Jinling Hospital, 305 Zhongshandong Road, Nanjing 210002, China.
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Zhao
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, Nanjing Jinling Hospital, 305 Zhongshandong Road, Nanjing 210002, China.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Chang
authors.affiliation: Department of Cardiology, The Third Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Jinling Road, Nanjing 210001, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
authors.affiliation: Department of Cardiology, The Third Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Jinling Road, Nanjing 210001, China.
authors.forename: Shou-Pei
authors.initials: SP
authors.lastname: Cao
authors.affiliation: Department of Cardiology, The Third Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Jinling Road, Nanjing 210001, China.
authors.forename: Meng-Ling
authors.initials: ML
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002316:Cardiotonic Agents / Q000494:pharmacology; D002460:Cell Line; D045304:Cytochromes c / Q000378:metabolism; D019610:Cytoprotection / Q000187:drug effects*; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006706:Homeostasis / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000633:toxicity; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D002316:Cardiotonic Agents; D005419:Flavonoids; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D048049:Extracellular Signal-Regulated MAP Kinases; D002118:Calcium; C056599:icariin
keywords: Cardiac apoptosis; ERK; Icariin; Mitochondria; ROS