title: TPGS-Stabilized Curcumin Nanoparticles Exhibit Superior Effect on Carrageenan-Induced Inflammation in Wistar Rat.
abstract: Curcumin, a hydrophobic polyphenol compound derived from the rhizome of the Curcuma genus, has a wide spectrum of biological and pharmacological applications. Previously, curcumin nanoparticles with different stabilizers had been produced successfully in order to enhance solubility and per oral absorption. In the present study, we tested the anti-inflammatory effect of d-α-Tocopheryl polyethylene glycol 1000 succinate (TPGS)-stabilized curcumin nanoparticles in vivo. Lambda-carrageenan (λ-carrageenan) was used to induce inflammation in rats; it was given by an intraplantar route and intrapelurally through surgery in the pleurisy test. In the λ-carrageenan-induced edema model, TPGS-stabilized curcumin nanoparticles were given orally one hour before induction and at 0.5, 4.5, and 8.5 h after induction with two different doses (1.8 and 0.9 mg/kg body weight (BW)). Sodium diclofenac with a dose of 4.5 mg/kg BW was used as a standard drug. A physical mixture of curcumin-TPGS was also used as a comparison with a higher dose of 60 mg/kg BW. The anti-inflammatory effect was assessed on the edema in the carrageenan-induced paw edema model and by the volume of exudate as well as the number of leukocytes reduced in the pleurisy test. TPGS-stabilized curcumin nanoparticles with lower doses showed better anti-inflammatory effects, indicating the greater absorption capability through the gastrointestinal tract.
authors.affiliation: Pharmaceutics Research Group, School of Pharmacy, Bandung Institute of Technology, Bandung 40132, Indonesia. h_rachmawati@fa.itb.ac.id.|Research Center for Nanosciences and Nanotechnology, Bandung Institute of Technology, Bandung 40132, Indonesia. h_rachmawati@fa.itb.ac.id.
authors.forename: Heni
authors.initials: H
authors.lastname: Rachmawati
authors.affiliation: Pharmacology and Clinical Pharmacy Research Group, School of Pharmacy, Bandung Institute of Technology, Bandung 40132, Indonesia. dewi.s@fa.itb.ac.id.
authors.forename: Dewi
authors.initials: D
authors.lastname: Safitri
authors.affiliation: Faculty of Pharmacy, University of Surabaya, Surabaya 60293, Indonesia. aditya_trias@yahoo.com.
authors.forename: Aditya Trias
authors.initials: AT
authors.lastname: Pradana
authors.affiliation: Pharmacology and Clinical Pharmacy Research Group, School of Pharmacy, Bandung Institute of Technology, Bandung 40132, Indonesia. ketut@fa.itb.ac.id.
authors.forename: I Ketut
authors.initials: IK
authors.lastname: Adnyana
keywords: Biopharmaceutical Class System (BCS) 4; curcumin; inflammation; nanoparticle; nanoparticle permeability; λ-carrageenan