title: [Dendritic Cells Promote the Proliferation of Peripheral Blood CRTH2 Cells (CD4(+)CD294(+)Th2) and Help B Cells to Secrete Immunoglobulin].
abstract: Objective：To investigate the promotive effect of dendritic cells(DCs) on proliferation of CRTH2 (CD4(+)CD294(+)Th2) cells and the influence of CRTH2 cells on secretion of immunoglobulin from B cells so as to provide a new approach for amplification and sorting of Th2 cells. Methods：DCs were induced from peripheral blood mononuclear cells, then the loaded-BCGV-Ag-DCs were cocultured with T cells, and the mixed lymphocyte reaction(MLR) was performed by CCK8 method. The phenotypes of DCs and CRTH2 cells were detected by flow cytometry. CRTH2 cells sorted by MACS were co-cultured with B cells for 5 days to detect the secretion of immunoglobulin. Results：The subsets and absolute number CRTH2 cells were significantly increased by loaded-BCGV-Ag-DCs. The levels of IgG, IgA and IgE were higher increased in supernatant of CRTH2 and B cell co-culture system than that in control group or that in transwell group(P<0.05). Conclusion：The proliferation of CRTH2 cells can be greatly promoted by loaded-BCGV-Ag-DCs, and the CRTH2 cells can help B cells to secrete IgG, IgA and IgE.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China; Department of Hematology, Longgang District People's Hospital of Shenzhen, Shenzhen 518172, Guangdong Province, China.
authors.forename: Fa-Qing
authors.initials: FQ
authors.lastname: Tian
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Hematology, Longgang District People's Hospital of Shenzhen, Shenzhen 518172, Guangdong Province, China.
authors.forename: Ju-Heng
authors.initials: JH
authors.lastname: Li
authors.affiliation: Department of Hematology, Longgang District People's Hospital of Shenzhen, Shenzhen 518172, Guangdong Province, China.
authors.forename: Mei-Qin
authors.initials: MQ
authors.lastname: Tang
authors.affiliation: Department of Hematology, Longgang District People's Hospital of Shenzhen, Shenzhen 518172, Guangdong Province, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Cheng
authors.affiliation: Blood Cell Laboratory, Longgang District People's Hospital of Shenzhen, Shenzhen 518172, Guangdong Province, China.
authors.forename: Ying-Cai
authors.initials: YC
authors.lastname: Huang
authors.affiliation: Blood Cell Laboratory, Longgang District People's Hospital of Shenzhen, Shenzhen 518172, Guangdong Province, China.
authors.forename: Hui-Qing
authors.initials: HQ
authors.lastname: Li
mesh_terms: D001402:B-Lymphocytes; D049109:Cell Proliferation; D018920:Coculture Techniques; D003713:Dendritic Cells; D005434:Flow Cytometry; D006801:Humans; D007136:Immunoglobulins; D007959:Lymphocyte Culture Test, Mixed; D018418:Th2 Cells
chemical_list: D007136:Immunoglobulins