title: Cytotoxic activity of Secondary Metabolites from Marine-derived Fungus Neosartorya siamensis in Human Cancer Cells.
abstract: Compounds isolated from the marine sea fan-derived fungus Neosartorya siamensis (KUFA 0017), namely, 2,4-dihydroxy-3-methylacetophenon (1), chevalone C (2), nortryptoquivaline (4), tryptoquivaline H (6), tryptoquivaline F (7), fiscalin A (8), epi-fiscalin A (9), epi-neofiscalin A (11) and epi-fiscalin C (13) were tested for anti-proliferative activity by MTT assay, DNA damage induction by comet assay, and induction of cell death by nuclear condensation assay on colon HCT116, liver HepG2 and melanoma A375 cancer cell lines. Compounds 2, 4, 8, 9, 11 and 13 presented IC50values ranging from 24 to 153 μM in the selected cell lines. Cell death was induced in HCT116 by compounds 2, 4 and 8. In HepG2, compounds 4, 8, 9 and 11 were able to induce significant cell death. This induction of cell death is possibly not related to genotoxicity because none of the compounds induced significant DNA damage. These results suggest that selected compounds present an interesting anti-proliferative activity and cell death induction, consequently showing potential (specifically epi-fiscalin C) as future leads for chemotherapeutic agents. Further studies on mechanisms of action should ensue. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Interdisciplinary Center for Marine and Environmental Research (CIIMAR), University of Porto (UPorto), Porto, Portugal.|Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto (UPorto), Porto, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Prata-Sena
authors.affiliation: Interdisciplinary Center for Marine and Environmental Research (CIIMAR), University of Porto (UPorto), Porto, Portugal.|Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto (UPorto), Porto, Portugal.
authors.forename: A A
authors.initials: AA
authors.lastname: Ramos
authors.affiliation: Interdisciplinary Center for Marine and Environmental Research (CIIMAR), University of Porto (UPorto), Porto, Portugal.|Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto (UPorto), Porto, Portugal.
authors.forename: S
authors.initials: S
authors.lastname: Buttachon
authors.affiliation: Interdisciplinary Center for Marine and Environmental Research (CIIMAR), University of Porto (UPorto), Porto, Portugal.|Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto (UPorto), Porto, Portugal.
authors.forename: B
authors.initials: B
authors.lastname: Castro-Carvalho
authors.affiliation: Interdisciplinary Center for Marine and Environmental Research (CIIMAR), University of Porto (UPorto), Porto, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Marques
authors.affiliation: Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand.
authors.forename: T
authors.initials: T
authors.lastname: Dethoup
authors.affiliation: Interdisciplinary Center for Marine and Environmental Research (CIIMAR), University of Porto (UPorto), Porto, Portugal.|Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto (UPorto), Porto, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Kijjoa
authors.affiliation: Interdisciplinary Center for Marine and Environmental Research (CIIMAR), University of Porto (UPorto), Porto, Portugal. erocha@icbas.up.pt.|Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto (UPorto), Porto, Portugal. erocha@icbas.up.pt.
authors.forename: E
authors.identifier: http://orcid.org/0000-0001-9813-4389
authors.initials: E
authors.lastname: Rocha
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy*; D055326:Neosartorya / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents
keywords: anticancer activity; cell death induction; cytotoxicity; fungi; human cancer cell lines; marine bioactive compounds