title: Data on the concentrations of etoposide, PSC833, BAPTA-AM, and cycloheximide that do not compromise the vitality of mature mouse oocytes, parthenogencially activated and fertilized embryos.
abstract: These data document the vitality of mature mouse oocytes (Metaphase II (MII)) and early stage embryos (zygotes) following exposure to the genotoxic chemotherapeutic agent, etoposide, in combination with PSC833, a selective inhibitor of permeability glycoprotein. They also illustrate the vitality of parthenogencially activated and fertilized embryos following incubation with the calcium chelator BAPTA-AM (1,2-Bis(2-aminophenoxy)ethane- N,N,N',N'-tetraacetic acid tetrakis (acetoxymethyl ester)), cycloheximide (an antibiotic that is capable of inhibiting protein synthesis), and hydrogen peroxide (a potent reactive oxygen species). Finally, they present evidence that permeability glycoprotein is not represented in the proteome of mouse spermatozoa. Our interpretation and discussion of these data feature in the article "Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes" (Martin et al., in press) [1].
authors.affiliation: Priority Research Centre for Reproductive Science, Discipline of Biological Sciences and Hunter Medical Research Institute, University of Newcastle, Callaghan, NSW 2308, Australia.
authors.forename: Jacinta H
authors.initials: JH
authors.lastname: Martin
authors.affiliation: Priority Research Centre for Reproductive Science, Discipline of Biological Sciences and Hunter Medical Research Institute, University of Newcastle, Callaghan, NSW 2308, Australia.
authors.forename: Elizabeth G
authors.initials: EG
authors.lastname: Bromfield
authors.affiliation: Priority Research Centre for Reproductive Science, Discipline of Biological Sciences and Hunter Medical Research Institute, University of Newcastle, Callaghan, NSW 2308, Australia.
authors.forename: R John
authors.initials: RJ
authors.lastname: Aitken
authors.affiliation: Priority Research Centre for Reproductive Science, Discipline of Biological Sciences and Hunter Medical Research Institute, University of Newcastle, Callaghan, NSW 2308, Australia.
authors.forename: Tessa
authors.initials: T
authors.lastname: Lord
authors.affiliation: Priority Research Centre for Reproductive Science, Discipline of Biological Sciences and Hunter Medical Research Institute, University of Newcastle, Callaghan, NSW 2308, Australia.
authors.forename: Brett
authors.initials: B
authors.lastname: Nixon
keywords: BAPTA-AM; Cyloheximide; Embryo; Etoposide; Oocytes; PSC833; Parthenote; Vitality