title: Non-cytochrome P450-mediated bioactivation and its toxicological relevance.
abstract: The bioactivation of drugs is often associated with toxicological outcomes; however, for most cases, the causal relationship between bioactivation and toxicity is not well established despite extensive research that attempts to elucidate the mechanisms leading to the formation of chemically reactive species, presumably the initial step towards adverse reactions. Due to rapid advancement in the research of cytochrome P450s (CYPs) and the prevalence of CYP involvement in the metabolic clearance of pharmaceuticals, CYP-mediated bioactivation is widely investigated and reviewed, while non-CYP-mediated bioactivation has not been emphasized. The widespread use of metabolic stability screening in drug discovery, however, has led to the identification of new chemical entities that rely on non-CYP enzymes for clearance, and the number of drugs that undergo metabolism via these enzymes has increased. Non-CYP enzymes can be divided into four general categories according to their enzymatic function, namely, oxidative, reductive, conjugative and hydrolytic. The aim of this review is to complement the existing literature on CYP-mediated metabolism by focusing on bioactivation mediated non-CYP enzymes and provide representative examples in each category.
authors.affiliation: a Pharmaceutical Candidate Optimization, Bristol-Myers Squibb , Princeton , NJ , USA.
authors.forename: Jinping
authors.initials: J
authors.lastname: Gan
authors.affiliation: b Department of Drug Metabolism & Pharmacokinetics, Genentech , South San Francisco , CA , USA.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Ma
authors.affiliation: b Department of Drug Metabolism & Pharmacokinetics, Genentech , South San Francisco , CA , USA.
authors.forename: Donglu
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D064420:Drug-Related Side Effects and Adverse Reactions; D006801:Humans; D006868:Hydrolysis; D050217:Metabolic Detoxication, Phase II; D010084:Oxidation-Reduction; D004364:Pharmaceutical Preparations / Q000378:metabolism*
chemical_list: D004364:Pharmaceutical Preparations; D003577:Cytochrome P-450 Enzyme System
keywords: Drug bioactivation; conjugation reactions; hydrolytic reactions; non-CYP enzymes; reductive reactions