title: Hybrid multispectral optoacoustic and ultrasound tomography for morphological and physiological brain imaging.
abstract: Expanding usage of small animal models in biomedical research necessitates development of technologies for structural, functional, or molecular imaging that can be readily integrated in the biological laboratory. Herein, we consider dual multispectral optoacoustic (OA) and ultrasound tomography based on curved ultrasound detector arrays and describe the performance achieved for hybrid morphological and physiological brain imaging of mice in vivo. We showcase coregistered hemodynamic parameters resolved by OA tomography under baseline conditions and during alterations of blood oxygen saturation. As an internal reference, we provide imaging of abdominal organs. We illustrate the performance advantages of hybrid curved detector ultrasound and OA tomography and discuss immediate and long-term implications of our findings in the context of animal and human studies.
authors.affiliation: Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Ingolstädter Landstraße 1, Neuherberg 85764, GermanybTechnische Universität München, School of Bioengineering, Boltzmannstraße 11, Garching 85748, Germany.
authors.forename: Ivan
authors.initials: I
authors.lastname: Olefir
authors.affiliation: Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Ingolstädter Landstraße 1, Neuherberg 85764, GermanycTechnische Universität München, Faculty of Medicine, Ismaninger Straße 22, Munich 81675, GermanydiThera Medical GmbH, Zielstattst.
authors.forename: Elena
authors.initials: E
authors.lastname: Mercep
authors.affiliation: iThera Medical GmbH, Zielstattstrasse 13, Munich 81379, Germany.
authors.forename: Neal C
authors.initials: NC
authors.lastname: Burton
authors.affiliation: Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Ingolstädter Landstraße 1, Neuherberg 85764, GermanybTechnische Universität München, School of Bioengineering, Boltzmannstraße 11, Garching 85748, Germany.
authors.forename: Saak V
authors.initials: SV
authors.lastname: Ovsepian
authors.affiliation: Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Ingolstädter Landstraße 1, Neuherberg 85764, GermanybTechnische Universität München, School of Bioengineering, Boltzmannstraße 11, Garching 85748, Germany.
authors.forename: Vasilis
authors.initials: V
authors.lastname: Ntziachristos
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging*; D006801:Humans; D051379:Mice; D059906:Neuroimaging / Q000295:instrumentation / Q000379:methods*; D061088:Photoacoustic Techniques; D014463:Ultrasonography