title: The Cell Wall-Associated Proteins in the Dimorphic Pathogenic Species of Paracoccidioides.
abstract: Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis (PCM). They are dimorphic ascomycetes that grow as filaments at mild temperatures up to 28oC and as multibudding pathogenic yeast cells at 37oC. Components of the fungal cell wall have an important role in the interaction with the host because they compose the cell outermost layer. The Paracoccidioides cell wall is composed mainly of polysaccharides, but it also contains proportionally smaller rates of proteins, lipids, and melanin. The polysaccharide cell wall composition and structure of Paracoccidioides yeast cells, filamentous and transition phases were studied in detail in the past. Other cell wall components have been better analyzed in the last decades. The present work gives to the readers a detailed updated view of cell wall-associated proteins. Proteins that have been localized at the cell wall compartment using antibodies are individually addressed. We also make an overview about PCM, the Paracoccidioides cell wall structure, secretion mechanisms, and fungal extracellular vesicles.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina- Universidade Federal de São Paulo (EPM-UNIFESP), Rua Botucatu, 862, oitavo andar, 04023-062, São Paulo, SP. Brazil.
authors.forename: Rosana
authors.initials: R
authors.lastname: Puccia
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina-Universidade Federal de São Paulo (EPM-UNIFESP), São Paulo, SP. Brazil.
authors.forename: Milene C
authors.initials: MC
authors.lastname: Vallejo
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina-Universidade Federal de São Paulo (EPM-UNIFESP), São Paulo, SP. Brazil.
authors.forename: Larissa V G
authors.initials: LVG
authors.lastname: Longo
mesh_terms: D000908:Antibodies, Fungal / Q000096:biosynthesis*; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D003165:Complement System Proteins / Q000235:genetics / Q000276:immunology; D000067128:Extracellular Vesicles / Q000737:chemistry / Q000276:immunology; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D005656:Fungal Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D006801:Humans; D007113:Immunity, Innate; D010228:Paracoccidioides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D010229:Paracoccidioidomycosis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology
chemical_list: D000908:Antibodies, Fungal; D062610:Fungal Polysaccharides; D005656:Fungal Proteins; D003165:Complement System Proteins
keywords: Paracoccidioides; cell wall; dmorphie; lipids; melanin.; proteins; secretion