title: The effect of heracleum persicum (Golpar) oil and alcoholic extracts on sperm parameters and chromatin quality in mice.
abstract: BACKGROUND
  Evaluating the significance and the effects of plant-derived drugs on laboratory animal's fertility was recognized. There was antioxidant activity reported from Heracleum persicum (Golpar).
  
  
  OBJECTIVE
  Current study aims to study the antioxidant effect of Golpar extracts on sperm parameters and chromatin quality in mice.
  
  
  METHODS
  Eighteen adult male mice were divided to 3 groups (10 wk old, 35 gr weight): group1 received hydro alcoholic extract (1000 mg/kg, ip), group 2 received oil extract (200 mg/kg, ip) and group 3 serving as the sham control group that received sterile water. Finally, left cauda epididymis of each animal was dissected and sperm analysis was done accordingly. To asses sperm chromatin and DNA quality, we used aniline blue (AB), toluidine blue (TB), chromomycin A3 (CMA3) and acridine orange (AO) staining.
  
  
  RESULTS
  Progressive and non-progressive sperm motility were significantly increased in group 1 in comparison with group 3 (p=0.032). There was an increasing trend in progressive sperm motility and decreasing trend in non-progressive sperm motility in group 2 in comparison with group 3, but the differences were not significant (p=0.221 and p=0.144, respectively). According to the sperm chromatin quality, the results of TB and AO tests revealed significant differences (p=0.004, p=0.000, respectively) between those groups and showed that the extracts of Golpar cause DNA damage, but no differences can be observed between them in AB and CMA3 staining (p>0.05).
  
  
  CONCLUSIONS
  The results showed that Heracleum persicum extracts may improve sperm motility. Also, it has harmful effects on sperm chromatin condensation and DNA integrity in mice.
authors.affiliation: Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Taghizabet
authors.affiliation: Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Esmat
authors.initials: E
authors.lastname: Mangoli
authors.affiliation: Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Anbari
authors.affiliation: School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Seyed Ali
authors.initials: SA
authors.lastname: Masoodi
authors.affiliation: Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Talebi
authors.affiliation: School of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Malihe
authors.initials: M
authors.lastname: Mazrooei
keywords: Chromatin; DNA integrity; Heracleum persicum extracts; Mice; Sperm