title: [Effect of peroxisome proliferator-activated receptor-γ coactivator-1α on metastasis and anoikis resistance in colorectal cancer].
abstract: OBJECTIVE
  To investigate the role of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) in the metastasis of colorectal cancer and underlying mechanisms.
  
  
  METHODS
  A colorectal cancer LoVo cell line transfected with a lentivirus vector stably expressinga shRNA targeting PGC-1αwas established. Cell proliferation was detected by CCK8 array. Migration and invasion were determined by Transwell assay. The expressions of epithelial-mesenchymal transition (EMT) markers were examined by western blot analysis.
  
  
  RESULTS
  Down-regulation of PGC-1α significantly inhibited the migration (74.4±4.5 versus 31.4±3.8,P<0.05), invasion (55.0±7.7 versus 17.6±5.0,P<0.05) and anoikis resistance (32.3±4.3)% versus (54.3±4.8)%,P<0.05, of LoVo cells. However, knockdown of PGC-1α had little effect on cell proliferation. Moreover, knockdown of PGC-1α induced EMT of LoVo cells by up-regulating E-cadherin and down-regulating vimentin. Alternatively, the expression of PGC-1α was induced by cell detachment. PGC-1αexpression was also higher in the colorectal cancer tissues than that in para-cancerous tissues, and its expression in the invading front area was higher than that in the tumor center area.
  
  
  CONCLUSIONS
  PGC-1α plays an important role in the metastasis of colorectal cancer, which may promote the invasion and anoikis resistance of colorectal cancer cells through EMT induction.
authors.affiliation: Department of Gastrointestinal Surgery, Nanjing Drum Tower Hospital, Nanjing 210008, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Lu
authors.affiliation: Department of Gastrointestinal Surgery, Nanjing Drum Tower Hospital, Nanjing 210008, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Xia
authors.affiliation: Department of Gastrointestinal Surgery, Nanjing Drum Tower Hospital, Nanjing 210008, China.
authors.forename: G
authors.initials: G
authors.lastname: Chen
mesh_terms: D023102:Anoikis / Q000502:physiology*; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D009361:Neoplasm Invasiveness; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034741:RNA, Small Interfering / Q000378:metabolism; D014157:Transcription Factors; D015854:Up-Regulation; D014746:Vimentin / Q000378:metabolism
chemical_list: D015820:Cadherins; C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D034741:RNA, Small Interfering; D014157:Transcription Factors; D014746:Vimentin