title: Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes.
abstract: Due to high resolution and reproducibility, chromatin immunoprecipitation (ChIP) has been used as a standard tool to investigate epigenetic marks including modified histones and specific histone variants (e.g., centromere-specific histone H3, CENH3) in this decade. Here, I describe a sensitive and low-background ChIP protocol for a wide range of plant species.
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan. nagaki@rib.okayama-u.ac.jp.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nagaki
mesh_terms: D047369:Chromatin Immunoprecipitation / Q000379:methods*; D044127:Epigenesis, Genetic; D009707:Nucleosomes / Q000235:genetics*; D010944:Plants / Q000235:genetics*
chemical_list: D009707:Nucleosomes
keywords: CENH3; Chromatin immunoprecipitation (ChIP); Epigenetic marks; Histone modification; Histone variants