title: Evaluation of Pax5 expression and comparison with BLA.36 and CD79αcy in feline non-Hodgkin lymphoma.
abstract: Paired box gene 5 (Pax5) is a widely used B-cell marker for human and canine non-Hodgkin's lymphoma (nHL); however, in the literature there is only one case report using Pax5 in a cat B-cell lymphoma. The purposes of this study were to investigate the expression and detection of B-cell specific activator protein (BSAP) using a monoclonal anti-Pax5 antibody in feline nHL (FnHL) tissue samples to evaluate its diagnostic relevance as a B-cell marker. A total of 45 FnHL samples in 45 cats were evaluated. B-cell lymphoma was the most common immunophenotype (51.1%) for all the samples and T-cell the most common immunophenotype (64.3%) for the gastrointestinal (GI) form. Pax5 stained 82.6% of all B-cell lymphomas and no expression was found in any of the T-cell lymphomas. Anti-Pax5 antibody staining in FnHL is similar to that reported in human and canine counterparts and may offer an excellent B-cell marker in cats.
authors.affiliation: Hospital Veterinário Berna, Onevet Group, Lisbon, Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Felisberto
authors.affiliation: Serviço de Anatomia Patológica, Instituto Português de Oncologia de Lisboa, Francisco Gentil, Lisbon, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Matos
authors.affiliation: Research Center for Biosciences & Health Technologies (CBIOS) / Faculdade de Medicina Veterinária, Universidade Lusófona de Humanidades e Tecnologias, Lisbon, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Alves
authors.affiliation: Serviço de Anatomia Patológica, Instituto Português de Oncologia de Lisboa, Francisco Gentil, Lisbon, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Cabeçadas
authors.affiliation: Hospital Veterinário Berna, Onevet Group, Lisbon, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Henriques
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D051925:CD79 Antigens / Q000378:metabolism*; D002371:Cat Diseases / Q000378:metabolism*; D002415:Cats; D005260:Female; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism / Q000662:veterinary*; D016399:Lymphoma, T-Cell / Q000378:metabolism / Q000662:veterinary; D008297:Male; D051757:PAX5 Transcription Factor / Q000378:metabolism*
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D017252:CD3 Complex; D051925:CD79 Antigens; D051757:PAX5 Transcription Factor
keywords: Pax5; expression; feline non-Hodgkin lymphoma; immunohistochemistry