title: Evaluation of the xenobiotic reaction against hyaluronate-based bioresorbable membrane in the abdominal cavity.
abstract: Postoperative abdominal adhesions are one of the most common post-laparotomy complications observed. Several types of adhesion preventative agents are available and their effectiveness and adverse impact have been clinically evaluated in previous studies. However, few basic studies have tested whether those agents do not trigger any unwanted xenobiotic reaction, which makes some surgeons hesitant to use them. To clarify this point, we investigated whether the adhesion preventative agent Seprafilm®(KAKEN PHARMACEUTICAL CO., LTD., Tokyo, Japan), one of the most widely used hyaluronate-based bioresorbable membrane (HBBM), can trigger an inflammatory response in normal abdominal tissue and delay the healing process. The rat underwent laparotomy and a HBBM was placed directly below the incision. Tissue samples at the incision and away from the incision (normal tissue) were harvested and inflammatory response and fibrosis were evaluated using quantitative PCR and histological scoring. We found that HBBM did not induce inflammatory cytokine expression at mRNA level in the peritoneal wall tissue or modify the fibrosis process in the abdominal cavity. These findings confirm the safety of using HBBM for the prevention of adhesion development post-laparotomy.
authors.affiliation: Division of Gastroenterology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho Chuo-ku, Kobe, Hyogo 650-0017 Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Nagata
authors.affiliation: Division of Gastroenterology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho Chuo-ku, Kobe, Hyogo 650-0017 Japan.
authors.forename: Namiko
authors.identifier: 0000-0003-2768-3991
authors.initials: N
authors.lastname: Hoshi
authors.affiliation: Division of Gastroenterology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho Chuo-ku, Kobe, Hyogo 650-0017 Japan ; Center for Clinical Research and Advanced Medicine Establishment Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga 520-2192 Japan.
authors.forename: Hayato
authors.initials: H
authors.lastname: Yoshinaka
authors.affiliation: Division of Gastroenterology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho Chuo-ku, Kobe, Hyogo 650-0017 Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Shiomi
authors.affiliation: Division of Gastroenterology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho Chuo-ku, Kobe, Hyogo 650-0017 Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Takenaka
authors.affiliation: Division of Gastroenterology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho Chuo-ku, Kobe, Hyogo 650-0017 Japan.
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Masuda
authors.affiliation: Division of Gastroenterology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho Chuo-ku, Kobe, Hyogo 650-0017 Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Maruyama
authors.affiliation: Division of Radiation Oncology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho Chuo-ku, Kobe, Hyogo 657-0017 Japan.
authors.forename: Ray
authors.initials: R
authors.lastname: Uchida
authors.affiliation: Division of Gastroenterology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho Chuo-ku, Kobe, Hyogo 650-0017 Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Azuma
authors.affiliation: Division of Gastroenterology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho Chuo-ku, Kobe, Hyogo 650-0017 Japan ; Center for Clinical Research and Advanced Medicine Establishment Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga 520-2192 Japan.
authors.forename: Hiromu
authors.initials: H
authors.lastname: Kutsumi
keywords: Antiadhesive; Foreign body reaction; HBBM; Peritoneal adhesion; Seprafilm®