title: G Protein-Coupled Receptors Incorporated into Rehydrated Diblock Copolymer Vesicles Retain Functionality.
abstract: G protein-coupled receptor (GPCR) is incorporated into polymeric vesicles made up of diblock copolymer bilayers. Successfully incorporated GPCRs exhibit correct biased physiological orientation and respond to various ligands. After extended dehydrated storage via lyophilization and subsequent rehydration, diblock copolymer polymersomes retain their shape and incorporated GPCR retains its function.
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 925 Bloom Walk, Los Angeles, CA, 90089, USA.
authors.forename: M Gertrude
authors.initials: MG
authors.lastname: Gutierrez
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 925 Bloom Walk, Los Angeles, CA, 90089, USA.
authors.forename: Farzad
authors.initials: F
authors.lastname: Jalali-Yazdi
authors.affiliation: Department of Chemical Engineering, University of Virginia, 102 Engineers' Way, P.O. Box 400741, Charlottesville, VA, 22904-4741, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Peruzzi
authors.affiliation: Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 925 Bloom Walk, Los Angeles, CA, 90089, USA.
authors.forename: Carson T
authors.initials: CT
authors.lastname: Riche
authors.affiliation: Mork Family Department of Chemical Engineering & Materials Science, Department of Chemistry, Department of Molecular & Computational Biology, and USC Norris Comprehensive Cancer Center, University of Southern California, 925 Bloom Walk, Los Angeles, CA, 90089, USA.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Roberts
authors.affiliation: Mork Family Department of Chemical Engineering & Materials Science, Department of Biomedical Engineering and Department of Chemistry, University of Southern California, 925 Bloom Walk, Los Angeles, CA, 90089, USA. malmstad@usc.edu.
authors.forename: Noah
authors.initials: N
authors.lastname: Malmstadt
mesh_terms: D005453:Fluorescence; D005612:Freeze Drying; D008051:Lipid Bilayers / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D044282:Receptor, Serotonin, 5-HT1A / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D012996:Solutions; D053835:Unilamellar Liposomes / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D011108:Polymers; D043562:Receptors, G-Protein-Coupled; D012996:Solutions; D053835:Unilamellar Liposomes; D044282:Receptor, Serotonin, 5-HT1A
keywords: GPCR; bilayer vesicles; block copolymers; lyophilization; polymersomes