title: Asymmetric Collagen/chitosan Membrane Containing Minocycline-loaded Chitosan Nanoparticles for Guided Bone Regeneration.
abstract: Infections caused by pathogens colonization at wound sites in the process of bone healing are considered as one of the major reasons for the failure of guided bone regeneration (GBR). The objective of this study was to prepare a novel asymmetric collagen/chitosan GBR membrane containing minocycline-loaded chitosan nanoparticles. The morphologies of the membranes and nanoparticles were observed by SEM and TEM, respectively. The characterization and biocompatibility of the membranes was evaluated. The effect of the membrane on bone regeneration was assessed using the critical-size at cranial defect model. TEM images showed the spherical morphology of the nanoparticles. The results of SEM indicated that the asymmetric membrane contained a dense collagen layer and a loose chitosan layer. An in vitro experiment showed that the membrane can inhibit bacterial growth and promote osteoblasts and fibroblasts growth. The membrane showed the ability to promote angiogenesis and enhance bone regeneration in vivo. An asymmetric collagen/chitosan GBR membrane can be fabricated by loading minocycline encapsulated chitosan nanoparticles, and shows satisfactory biocompatibility and barrier function, which enhances bone regeneration. Therefore, this antibacterial GBR membrane is a promising therapeutic approach to prevent infection and guide bone regeneration.
authors.affiliation: School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, PR China.
authors.forename: Shiqing
authors.initials: S
authors.lastname: Ma
authors.affiliation: School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, PR China.
authors.forename: Aidina
authors.initials: A
authors.lastname: Adayi
authors.affiliation: School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, PR China.
authors.forename: Zihao
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, PR China.
authors.forename: Mingyao
authors.initials: M
authors.lastname: Wu
authors.affiliation: School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, PR China.
authors.forename: Linghao
authors.initials: L
authors.lastname: Xiao
authors.affiliation: School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, PR China.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Sun
authors.affiliation: The Key Laboratory of Beijing City on Preparation and Processing of Novel Polymer, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Cai
authors.affiliation: The Key Laboratory of Beijing City on Preparation and Processing of Novel Polymer, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yang
authors.affiliation: School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Gao
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D001861:Bone Regeneration / Q000187:drug effects*; D048271:Chitosan / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D048091:Guided Tissue Regeneration / Q000379:methods*; D008297:Male; D008422:Materials Testing; D008567:Membranes, Artificial; D008911:Minocycline / Q000494:pharmacology*; D053758:Nanoparticles / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000900:Anti-Bacterial Agents; D008567:Membranes, Artificial; D003094:Collagen; D048271:Chitosan; D008911:Minocycline