title: Ascitic Fluid Culture in Cirrhotic Patients with Spontaneous Bacterial Peritonitis.
abstract: OBJECTIVE
  To determine the frequency and compare the culture yield of bacterial isolation by conventional and blood culture BACTEC bottle techniques in cirrhotic patients with spontaneous bacterial peritonitis (SBP).
  
  
  METHODS
  Cross-sectional comparative study.
  
  
  METHODS
  Pathology Department, Bannu Medical College, Bannu, KPK, from January 2012 to December 2013.
  
  
  METHODS
  Paracentesis of 20 ml of ascitic fluid tapped from cirrhotic patients with SBPwas carried out by a single technologist. The analysis included differential leukocyte count (DLC), while 5 ml each of the fluid was inoculated into conventional culture media and BACTEC blood culture bottle. All the data were analysed on (SPSS) version 16 to determine frequencies with percentages and mean values with standard deviation. Chi-square test was used for comparing the yield of conventional and blood culture bottle methods. P-value was considered significant if < 0.05.
  
  
  RESULTS
  In 105 cases of ascitic fluid analyses, 27 (25.72%) had positive ascitic fluid culture whereas 78 (74.28%) had negative ascitic fluid culture. Ascitic fluid culture was positive in 6 cases by conventional culture media and in 27 cases by BACTEC culture bottle media (p < 0.001). Bacterial isolation was obtained by both culture methods in 6 cases (p < 0.001).
  
  
  CONCLUSIONS
  Direct bedside inoculation of ascitic fluid by BACTEC culture bottle method has better yield as compared to conventional culture method.
authors.affiliation: Department of Pathology, Bannu Medical College, Bannu, KPK, Pakistan.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Sajjad
authors.affiliation: Department of Pathology, Bannu Medical College, Bannu, KPK, Pakistan.
authors.forename: Zard Ali
authors.initials: ZA
authors.lastname: Khan
authors.affiliation: Department of Pathology, Bannu Medical College, Bannu, KPK, Pakistan.
authors.forename: Mohammad Shoaib
authors.initials: MS
authors.lastname: Khan
mesh_terms: D000328:Adult; D000368:Aged; D001202:Ascitic Fluid / Q000382:microbiology*; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000150:complications / Q000382:microbiology*; D001431:Bacteriological Techniques; D003430:Cross-Sectional Studies; D003470:Culture Media; D004926:Escherichia coli / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007711:Klebsiella pneumoniae / Q000302:isolation & purification; D008103:Liver Cirrhosis / Q000150:complications*; D008875:Middle Aged; D010154:Pakistan; D010538:Peritonitis / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology*; D011549:Pseudomonas / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000302:isolation & purification
chemical_list: D003470:Culture Media