title: A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats.
abstract: A simple, accurate, and reproducible high-performance liquid chromatography (HPLC) method has been developed and validated for the quantification of quercetin (QR) in rat plasma. The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the internal standard. The chromatographic analysis was achieved on a Shimadzu LC 20 A HPLC system equipped with a Supelcosil LC-18 T C18column and an isocratic mobile phase consisting of 0.3% trichloroacetic acid in water and acetonitrile HPLC-grade (50:50, v/v) run at a flow rate of 0.9 mL/min for 13 min. The UV detection wavelength was set at 254 nm. The method exhibited good linearity (R2> 0.994) over the assayed concentration range (0.10-25 μg/mL) and demonstrated good intra-day and inter-day precision and accuracy (relative standard deviations and the deviation from predicted values were <20%). This method was also successfully applied for studying the pharmacokinetics of QR in rats following a single oral dose of QR to evaluate its pharmacokinetic parameters in rats.
authors.affiliation: Faculty of Pharmacy, Kafrelsheikh University, Egypt.
authors.forename: Khaled S
authors.initials: KS
authors.lastname: Abdelkawy
authors.affiliation: School of Pharmacy, Pacific University, Hillsboro, OR, USA.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Balyshev
authors.affiliation: School of Pharmacy, Pacific University, Hillsboro, OR, USA.
authors.forename: Fawzy
authors.initials: F
authors.lastname: Elbarbry
mesh_terms: D000818:Animals; D016227:Benzoquinones / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D011794:Quercetin / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D015203:Reproducibility of Results
chemical_list: D016227:Benzoquinones; D011794:Quercetin; C003466:thymoquinone
keywords: HPLC; pharmacokinetics; quercetin; rats; thymoquinone; validation