title: Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake.
abstract: Mitogen-activated protein kinase kinase kinase 5 (MAP3K5) is essential for apoptosis, proliferation, differentiation, and immune responses, and is a candidate marker for residual feed intake (RFI) in pig. We cloned the full-length cDNA sequence of porcine MAP3K5 by rapid-amplification of cDNA ends. The 5451-bp gene contains a 5'-untranslated region (UTR) (718 bp), a coding region (3738 bp), and a 3'-UTR (995 bp), and encodes a peptide of 1245 amino acids, which shares 97, 99, 97, 93, 91, and 84% sequence identity with cattle, sheep, human, mouse, chicken, and zebrafish MAP3K5, respectively. The deduced MAP3K5 protein sequence contains two conserved domains: a DUF4071 domain and a protein kinase domain. Phylogenetic analysis showed that porcine MAP3K5 forms a separate branch to vicugna and camel MAP3K5. Tissue expression analysis using real-time quantitative polymerase chain reaction (qRT-PCR) revealed that MAP3K5 was expressed in the heart, liver, spleen, lung, kidney, muscle, fat, pancrea, ileum, and stomach tissues. Copy number variation was detected for porcine MAP3K5 and validated by qRT-PCR. Furthermore, a significant increase in average copy number was detected in the low RFI group when compared to the high RFI group in a Duroc pig population. These results provide useful information regarding the influence of MAP3K5 on RFI in pigs.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: L
authors.initials: L
authors.lastname: Pu
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: L C
authors.initials: LC
authors.lastname: Zhang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: J S
authors.initials: JS
authors.lastname: Zhang
authors.affiliation: Research Institute of Truein Agro-Pastoral Group Co., Ltd., Kaifeng, China.
authors.forename: X
authors.initials: X
authors.lastname: Song
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: L G
authors.initials: LG
authors.lastname: Wang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: J
authors.initials: J
authors.lastname: Liang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Y B
authors.initials: YB
authors.lastname: Zhang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: H
authors.initials: H
authors.lastname: Yan
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: J W
authors.initials: JW
authors.lastname: Yue
authors.affiliation: Jilin Academy of Agricultural Sciences, Changchun, China.
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Animal Science Technology, Hunan Agricultural University, Changsha, China.
authors.forename: Q Q
authors.initials: QQ
authors.lastname: Wu
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China iaswlx@263.net.
authors.forename: L X
authors.initials: LX
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020134:Catalytic Domain; D003001:Cloning, Molecular; D056915:DNA Copy Number Variations; D018628:Gene Dosage; D015870:Gene Expression; D057927:Hydrophobic and Hydrophilic Interactions; D048848:MAP Kinase Kinase Kinase 5 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D009928:Organ Specificity; D010802:Phylogeny; D000072756:Protein Conformation, alpha-Helical; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism
chemical_list: D048848:MAP Kinase Kinase Kinase 5