title: Ultrasonic-assisted green synthesis of flower like silver nanocolloids using marine sponge extract and its effect on oral biofilm bacteria and oral cancer cell lines.
abstract: The knowhow followed for synthesis, characterization and application of nanomaterials has become an important branch of nanoscience. The use of marine sponges for the synthesis of metal nanoparticles is still in the budding level of current nanobiotechnology. This paper reports a single step one pot biosynthesis utilizing marine sponge (Haliclona exigua) extract as a reducing agent by means of a conventional ultrasonic bath on the formation and growth of flower like silver nanocolloids. These silver nanocolloids were characterized through UV visible spectroscopy, High Resolution Transmission Electron Microscope, Fourier Transform Infrared spectroscopy and X-ray Diffractometer. Further, antibacterial activity and antiproliferative activity were done against oral biofilm bacteria and oral cancer cell lines for the biosynthesized flower like silver nanocolloids. Water soluble organic amines were responsible for the syntheses of nanomaterials which have a size range from 100 to 120 nm. An average size of 9.1 mm zone of inhibition was recorded with 10.0 μg of silver nanocolloids against oral biofilm bacteria. The estimated half maximal inhibitory concentration value for flower like silver nanocolloids was 0.6 μg/ml for oral cancer cell lines.
authors.affiliation: Centre for Ocean Research, Sathyabama University, Chennai, 600 119, India. Electronic address: inbakandan@gmail.com.
authors.forename: D
authors.initials: D
authors.lastname: Inbakandan
authors.affiliation: Centre for Ocean Research, Sathyabama University, Chennai, 600 119, India.
authors.forename: C
authors.initials: C
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Sathyabama University, Chennai, 600 119, India.
authors.forename: M
authors.initials: M
authors.lastname: Bavanilatha
authors.affiliation: Department of Biotechnology, Sathyabama University, Chennai, 600 119, India.
authors.forename: Dune Naga
authors.initials: DN
authors.lastname: Ravindra
authors.affiliation: Earth System Sciences Organization - National Institute of Ocean Technology, Pallikaranai, Chennai, 600 100, India.
authors.forename: R
authors.initials: R
authors.lastname: Kirubagaran
authors.affiliation: Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai, 608 502, India.
authors.forename: S Ajmal
authors.initials: SA
authors.lastname: Khan
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology*; D059001:Aquatic Organisms / Q000737:chemistry; D001419:Bacteria / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D002457:Cell Extracts / Q000302:isolation & purification; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D011161:Porifera / Q000737:chemistry*; D012834:Silver / Q000378:metabolism* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013057:Spectrum Analysis; D000069453:Ultrasonic Waves; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D002457:Cell Extracts; D012834:Silver
keywords: Antibacterial; Biosynthesis; Oral biofilms; Oral cancer cells; Silver nanoparticles; Ultrasonic