title: Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: an update.
abstract: The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant response against the free radicals produced in a given disease. Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC) are different assays described to determine TAC of a sample. This review explains the basis of each assay and their application in the determination of TAC in dogs, and also provides selected information about reports in humans for comparative purposes. It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, 30100, Espinardo, Murcia, Spain.
authors.forename: Camila Peres
authors.initials: CP
authors.lastname: Rubio
authors.affiliation: Department of Plant Biology (Plant Physiology), Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: Josefa
authors.initials: J
authors.lastname: Hernández-Ruiz
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, 30100, Espinardo, Murcia, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Martinez-Subiela
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, 30100, Espinardo, Murcia, Spain.|Department of Animal Medicine and Surgery, Veterinary School, University Autonoma of Barcelona, Barcelona, Spain.
authors.forename: Asta
authors.initials: A
authors.lastname: Tvarijonaviciute
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, 30100, Espinardo, Murcia, Spain. jjceron@um.es.
authors.forename: José Joaquin
authors.initials: JJ
authors.lastname: Ceron
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001774:Blood Chemical Analysis / Q000379:methods*; D004285:Dogs / Q000097:blood*; D006801:Humans; D013053:Spectrophotometry / Q000662:veterinary*; D014730:Veterinary Medicine / Q000639:trends
chemical_list: D000975:Antioxidants
keywords: Antioxidant activity; Canine serum; Cupric; Ferric; Human serum; Oxidative stress; Trolox