title: The effects of 4-MEI on cell proliferation, DNA breaking and DNA fragmentation.
abstract: 4-Methylimidazole (4-MEI) is a color widely found in cola drinks, roasted foods, grilled meats, coffee and other foods. This study was aimed to investigate the 4-MEI effects on the cell proliferation, purified circular DNA and DNA from cells of rats treated with the 4-MEI.In this study, mouse 3T3-L1 cell line was treated with 4-MEI at concentrations of 300, 450, 600 and 750 µg/mL for 24 hours and 48 hours periods, after that cytotoxic effect of the 4-MEI was studied by MTT test. Also, the effect of 4-MEI on purified circular DNA (pET22b) was investigated by treating of the DNA with 4-MEI concentrations of 300, 450, 600 and 750 µg/ml. DNA was extracted from liver cells of rats that have been treated with 4-MEI doses of 25 and 50 mg/kg for 10 week and it was subjected to agarose gel electrophoreses analyses.4-MEI significantly inhibited cell proliferation of 3T3-L1 cell line at highest concentration for 24 h and at all concentration for 48 h treatment time. DNA fragmentation assay showed that 4-MEI at 50 mg/kg concentration clearly produced characteristic DNA smear and no DNA laddering (200bp) was observed when mouse was exposed to 4-MEI. The results obtained from plasmid DNA damaging assay showed that 4-MEI has noeffect on the DNA, because the electrophoretic pattern of DNA treated with 4-MEI showed three bands on agarose gel electrophoresis as it was for untreated control. 4-MEI showed cytotoxic effect on 3T3-L1 cells but no effect on plasmid DNA breaking. According to DNA fragmentation assay 4-MEI has necrosis effects on mouse liver cells (Tab. 1, Fig. 4, Ref. 27).
authors.forename: M Norizadeh
authors.initials: MN
authors.lastname: Tazehkand
authors.forename: A
authors.initials: A
authors.lastname: Moridikia
authors.forename: O
authors.initials: O
authors.lastname: Hajipour
authors.forename: E
authors.initials: E
authors.lastname: Valipour
authors.forename: T
authors.initials: T
authors.lastname: Timocin
authors.forename: M
authors.initials: M
authors.lastname: Topaktas
authors.forename: M B
authors.initials: MB
authors.lastname: Yilmaz
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D004247:DNA; D053960:DNA Breaks / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects*; D007093:Imidazoles / Q000494:pharmacology*; D008297:Male; D008460:Meat; D051379:Mice; D051381:Rats
chemical_list: D007093:Imidazoles; D004247:DNA; C023439:4-methylimidazole
keywords: 4-Methylimidazole; DNA fragmentation; MTT; plasmid DNA breaking.