title: Water-soluble polysaccharides and hemicelluloses from almond gum: Functional and prebiotic properties.
abstract: This paper describes the extraction of polysaccharides (AGP) and hemicelluloses (AGH) from almond gum by hot water and alkaline solution, respectively. Structural and functional properties of the extracted polymers were then determined. For this purpose, infrared spectroscopy was first used to characterize functional groups of both polymers. The molecular weights of AGP and AGH were then determined using high performance size exclusion chromatography, resulting in 5.72×106g/mol and 5.39×106g/mol, respectively. Monosaccharide composition of both polymers was assessed using gas chromatography. The analysis of the functional properties showed that AGP and AGH had high water-holding (11.36g/g and 6.3g/g, respectively) and fat-binding (5.35g/g and 2.7g/g, respectively) capacities, with good emulsion properties. The prebiotic properties of AGP and AGH were then evaluated using in vitro fermentation by Bifidobacterium adolescentis and Lactobacillus acidophilus. Both polymers showed suitability for in vitro fermentation, suggesting thus their prebiotic nature. The obtained results demonstrated the promising potential of AGP and AGH for different applications in food industry.
authors.affiliation: Enzyme & Bioconversion Unit (UR13ES74), National School of Engineering, P.O. Box 1173, 3038, Sfax University, Tunisia. Electronic address: fatma.bouaziz22@yahoo.fr.
authors.forename: Fatma
authors.initials: F
authors.lastname: Bouaziz
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, Laboratoire Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Centre de Recherche de Royallieu, CS 60319, 60203 Compiègne Cedex, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Koubaa
authors.affiliation: Enzyme & Bioconversion Unit (UR13ES74), National School of Engineering, P.O. Box 1173, 3038, Sfax University, Tunisia.
authors.forename: Khawla
authors.initials: K
authors.lastname: Ben Jeddou
authors.affiliation: Enzyme & Bioconversion Unit (UR13ES74), National School of Engineering, P.O. Box 1173, 3038, Sfax University, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Kallel
authors.affiliation: Service de chromatographie, purification et analyse de polysaccharides CERMAV-CNRS, 601 rue de la Chimie, 38041 Grenoble, Cedex 9, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Boisset Helbert
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, Laboratoire Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Centre de Recherche de Royallieu, CS 60319, 60203 Compiègne Cedex, France.
authors.forename: Anissa
authors.initials: A
authors.lastname: Khelfa
authors.affiliation: Enzyme & Bioconversion Unit (UR13ES74), National School of Engineering, P.O. Box 1173, 3038, Sfax University, Tunisia.
authors.forename: Rhoudha
authors.initials: R
authors.lastname: Ellouz Ghorbel
authors.affiliation: Enzyme & Bioconversion Unit (UR13ES74), National School of Engineering, P.O. Box 1173, 3038, Sfax University, Tunisia; Common service unit of bioreactor coupled with an ultrafilter, National School of Engineering, P.O. Box 1173, 3038, Sfax University, Tunisia.
authors.forename: Semia
authors.initials: S
authors.lastname: Ellouz Chaabouni
mesh_terms: D001644:Bifidobacterium / Q000254:growth & development*; D007779:Lactobacillus acidophilus / Q000254:growth & development*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D056692:Prebiotics; D000068181:Prunus dulcis / Q000737:chemistry*; D012995:Solubility
chemical_list: D011134:Polysaccharides; D056692:Prebiotics; C007916:hemicellulose
keywords: Almond gum polysaccharide; Functional properties; Hemicellulose