title: Correlation of Checkerboard Synergy Testing with Time-Kill Analysis and Clinical Outcomes of Extensively Drug-Resistant Acinetobacter baumannii Respiratory Infections.
abstract: We tested 76 extensively drug-resistant (XDR) Acinetobacter baumannii isolates by the checkerboard method using only wells containing serum-achievable concentrations (SACs) of drugs. Checkerboard results were correlated by time-kill assay and clinical outcomes. Minocycline-colistin was the best combination in vitro, as it inhibited growth in one or more SAC wells in all isolates. Patients who received a combination that inhibited growth in one or more SAC wells demonstrated better microbiological clearance than those who did not (88% versus 30%; P = 0.025). The checkerboard platform may have clinical utility for XDR A. baumannii infections.
authors.affiliation: Department of Pharmacy, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.
authors.forename: Derek N
authors.initials: DN
authors.lastname: Bremmer
authors.affiliation: Department of Pharmacy, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.
authors.forename: Karri A
authors.initials: KA
authors.lastname: Bauer
authors.affiliation: Division of Infectious Diseases, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Pouch
authors.affiliation: Department of Pathology, The Ohio State University Medical Center, Columbus, Ohio, USA.
authors.forename: Keelie
authors.initials: K
authors.lastname: Thomas
authors.affiliation: Department of Pathology, The Ohio State University Medical Center, Columbus, Ohio, USA.
authors.forename: Debra
authors.initials: D
authors.lastname: Smith
authors.affiliation: Department of Pharmacy, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.
authors.forename: Debra A
authors.initials: DA
authors.lastname: Goff
authors.affiliation: Department of Pathology, The Ohio State University Medical Center, Columbus, Ohio, USA.
authors.forename: Preeti
authors.initials: P
authors.lastname: Pancholi
authors.affiliation: Department of Pathology, The Ohio State University Medical Center, Columbus, Ohio, USA Joan.balada@osumc.edu.
authors.forename: Joan-Miquel
authors.initials: JM
authors.lastname: Balada-Llasat
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000472:pathogenicity; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000188:drug therapy / Q000382:microbiology; D003091:Colistin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods*; D008875:Middle Aged; D008911:Minocycline / Q000494:pharmacology; D018410:Pneumonia, Bacterial / Q000188:drug therapy / Q000382:microbiology; D012141:Respiratory Tract Infections / Q000188:drug therapy* / Q000382:microbiology; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D008911:Minocycline; D003091:Colistin