title: The Need for Integrated Approaches in Metabolic Engineering.
abstract: This review highlights state-of-the-art procedures for heterologous small-molecule biosynthesis, the associated bottlenecks, and new strategies that have the potential to accelerate future accomplishments in metabolic engineering. We emphasize that a combination of different approaches over multiple time and size scales must be considered for successful pathway engineering in a heterologous host. We have classified these optimization procedures based on the "system" that is being manipulated: transcriptome, translatome, proteome, or reactome. By bridging multiple disciplines, including molecular biology, biochemistry, biophysics, and computational sciences, we can create an integral framework for the discovery and implementation of novel biosynthetic production routes.
authors.affiliation: Joint Bioenergy Institute (JBEI), Emeryville, California 94608.|Department of Chemical & Biomolecular Engineering, Department of Bioengineering, University of California, Berkeley, California 94720.
authors.forename: Anna
authors.initials: A
authors.lastname: Lechner
authors.affiliation: Department of Bioengineering, University of California, San Diego, California 92093.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Brunk
authors.affiliation: Joint Bioenergy Institute (JBEI), Emeryville, California 94608.|Department of Chemical & Biomolecular Engineering, Department of Bioengineering, University of California, Berkeley, California 94720.|Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720.
authors.forename: Jay D
authors.initials: JD
authors.lastname: Keasling
mesh_terms: D060847:Metabolic Engineering; D059467:Transcriptome