title: Efficient Generation of Viral and Integration-Free Human Induced Pluripotent Stem Cell-Derived Oligodendrocytes.
abstract: Here we document three highly reproducible protocols: (1) a culture system for the derivation of human oligodendrocytes (OLs) from human induced pluripotent stem cells (hiPS) and their further maturation-our protocol generates viral- and integration-free OLs that efficiently commit and move forward in the OL lineage, recapitulating all the steps known to occur during in vivo development; (2) a method for the isolation, propagation and maintenance of neural stem cells (NSCs); and (3) a protocol for the production, isolation, and maintenance of OLs from perinatal rodent and human brain-derived NSCs. Our unique culture systems rely on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of OL as they advance from OL progenitors to mature, myelinating cells. We are confident that these protocols bring our field a step closer to efficient autologous cell replacement therapies and disease modeling. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience, David Geffen School of Medicine at UCLA, Los Angeles, California.
authors.forename: Araceli
authors.initials: A
authors.lastname: Espinosa-Jeffrey
authors.affiliation: Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience, David Geffen School of Medicine at UCLA, Los Angeles, California.
authors.forename: Bruno
authors.initials: B
authors.lastname: Blanchi
authors.affiliation: Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, California.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Biancotti
authors.affiliation: Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience, David Geffen School of Medicine at UCLA, Los Angeles, California.
authors.forename: Shalini
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience, David Geffen School of Medicine at UCLA, Los Angeles, California.
authors.forename: Megumi
authors.initials: M
authors.lastname: Hirose
authors.affiliation: Regenerative Medicine Institute, Cedars Sinai Medical Center, Los Angeles, California.
authors.forename: Berhan
authors.initials: B
authors.lastname: Mandefro
authors.affiliation: Regenerative Medicine Institute, Cedars Sinai Medical Center, Los Angeles, California.
authors.forename: Dodanim
authors.initials: D
authors.lastname: Talavera-Adame
authors.affiliation: Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Nissim
authors.initials: N
authors.lastname: Benvenisty
authors.affiliation: Intellectual and Developmental Disabilities Research Center, Semel Institute for Neuroscience, David Geffen School of Medicine at UCLA, Los Angeles, California.
authors.forename: Jean
authors.initials: J
authors.lastname: de Vellis
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000196:embryology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage; D002470:Cell Survival; D002999:Clone Cells; D004475:Ectoderm / Q000166:cytology; D058732:Embryoid Bodies / Q000166:cytology; D005333:Fetus / Q000166:cytology; D005615:Freezing; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology; D009474:Neurons / Q000166:cytology; D009836:Oligodendroglia / Q000166:cytology*; D051381:Rats; D033581:Stem Cell Transplantation; D014780:Viruses / Q000378:metabolism*
keywords: NSC; chemically defined media; human induced pluripotent stem cells; lineage progression; neural stem cells; neurospheres; oligodendrocyte maturation; oligodendrocyte specification; oligospheres