title: Molecular Characterization of High-Risk Human Papillomavirus in Women in Bobo-Dioulasso, Burkina Faso.
abstract: High-risk human papillomavirus (HPV) is found in over 99% of cervical cancers. The aim of this study was to determine the prevalence of HPV in a population of women in Bobo-Dioulasso and to identify the high-risk types present in these women. From May to June, 2015, 181 women who came for consultation at the Souro Sanou University Hospital of Bobo-Dioulasso have been included in this study. Uterine endocervical swabs have been taken in these women. DNA obtained by extraction from the samples thus collected was used to determine the prevalence of high-risk human papillomavirus genotypes through real-time PCR. The age of the women ranged from 20 to 56 years with a mean of 35.3 ± 8.1 years. The prevalence of infection by high-risk HPV types was 25.4% (46/181). The most common high-risk HPV genotypes were HPV 39 (18.5%), HPV 52 (16.7%), HPV 18 (14.8%), and HPV 35 (13.0%). HPV 16 which is included in the HPV vaccines was not found in the population studied. This type of study which is the first one in Bobo-Dioulasso has showed a high prevalence of genotypes HPV 39, HPV 52, and HPV 35 which are not yet covered by a vaccine.
authors.affiliation: Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, P.O. Box 7021, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), P.O. Box 364, Ouagadougou, Burkina Faso.
authors.forename: Ina Marie Angèle
authors.initials: IM
authors.lastname: Traore
authors.affiliation: Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, P.O. Box 7021, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), P.O. Box 364, Ouagadougou, Burkina Faso.
authors.forename: Théodora Mahoukèdè
authors.identifier: 0000-0003-3696-8972
authors.initials: TM
authors.lastname: Zohoncon
authors.affiliation: Department of Gynecology, Obstetrics and Reproductive Medicine, Sourou Sanou Teaching Hospital, P.O. Box 676, Bobo-Dioulasso, Burkina Faso.
authors.forename: Adama
authors.initials: A
authors.lastname: Dembele
authors.affiliation: Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, P.O. Box 7021, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), P.O. Box 364, Ouagadougou, Burkina Faso.
authors.forename: Florencia W
authors.identifier: 0000-0002-6895-6725
authors.initials: FW
authors.lastname: Djigma
authors.affiliation: School of Medical Sciences, Microbiology Department, University of Cape Coast, Cape Coast, Ghana.
authors.forename: Dorcas
authors.initials: D
authors.lastname: Obiri-Yeboah
authors.affiliation: Polyvalent Medical Center-Health and Reproduction, P.O. Box 1418, Bobo-Dioulasso, Burkina Faso.
authors.forename: Germain
authors.initials: G
authors.lastname: Traore
authors.affiliation: Department of Gynecology, Obstetrics and Reproductive Medicine, Sourou Sanou Teaching Hospital, P.O. Box 676, Bobo-Dioulasso, Burkina Faso.
authors.forename: Moussa
authors.initials: M
authors.lastname: Bambara
authors.affiliation: Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, P.O. Box 7021, Ouagadougou, Burkina Faso; Yalgado Ouédraogo University Hospital (CHU/YO), P.O. Box 7022, Ouagadougou, Burkina Faso; UFR/SDS, University of Ouagadougou, P.O. Box 7021, Ouagadougou, Burkina Faso.
authors.forename: Charlemagne
authors.initials: C
authors.lastname: Ouedraogo
authors.affiliation: Training and Research Unit in Life and Earth Science (UFR/SVT), University of Ouagadougou, P.O. Box 7021, Ouagadougou, Burkina Faso.
authors.forename: Yves
authors.initials: Y
authors.lastname: Traore
authors.affiliation: Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, P.O. Box 7021, Ouagadougou, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), P.O. Box 364, Ouagadougou, Burkina Faso; Faculty of Medicine, University Saint Thomas d'Aquin, P.O. Box 10212, Ouagadougou, Burkina Faso.
authors.forename: Jacques
authors.identifier: 0000-0002-0415-9161
authors.initials: J
authors.lastname: Simpore
mesh_terms: D000328:Adult; D002050:Burkina Faso / Q000453:epidemiology; D003710:Demography; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000235:genetics*; D015995:Prevalence; D012307:Risk Factors; D012725:Sexual Behavior; D055815:Young Adult