title: Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana.
abstract: Artificial production of haploids is one of the important sought-after goals of plant breeding and crop improvement programs. Conventionally, haploid plants are generated by in vitro (tissue) culture of haploid plant gametophytes, pollen (male), and embryo sac (female). Here, we describe a facile, nontissue culture-based in vivo method of haploid production through seeds in the model plant, Arabidopsis thaliana. This method involves simple crossing of any desired genotype of interest to a haploid-inducing strain (GFP-tailswap) to directly obtain haploid F1 seeds. The described protocol can be practiced by anyone with basic experience in growing A. thaliana plants and will be of interest to Arabidopsis research community.
authors.affiliation: School of Biology, Indian Institute of Science Education and Research (IISER)-Thiruvananthapuram, Thiruvananthapuram, Kerala, India. ravi@iisertvm.ac.in.
authors.forename: Maruthachalam
authors.initials: M
authors.lastname: Ravi
authors.affiliation: School of Biology, Indian Institute of Science Education and Research (IISER)-Thiruvananthapuram, Thiruvananthapuram, Kerala, India.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Bondada
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics; D003078:Colchicine / Q000494:pharmacology; D018744:DNA, Plant / Q000302:isolation & purification; D018745:Genome, Plant; D006238:Haploidy; D006657:Histones / Q000235:genetics; D000069600:Plant Breeding / Q000379:methods*; D018517:Plant Roots / Q000235:genetics; D030821:Plants, Genetically Modified / Q000254:growth & development; D011058:Pollen / Q000235:genetics; D036226:Seedlings / Q000235:genetics; D012639:Seeds / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D006657:Histones; D003078:Colchicine
keywords: Arabidopsis; CenH3; Centromeres; GFP-tailswap; Genome elimination; Haploid; In vivo haploid; SeedGFP-HI; Seeds