title: Distinctive Leukocyte Subpopulations According to Organ Type in Cynomolgus Macaques.
abstract: Cynomolgus macaques (CYNO; Macaca fascicularis) are a well-established NHP model used for studies in immunology. To provide reference values on the baseline cell distributions in the hematopoietic and lymphoid organs (HLO) of these animals, we used flow cytometry to analyze the peripheral blood, bone marrow, mesenteric lymph nodes, spleen, and thymus of a cohort of male, adult, research-naïve, Mauritian CYNO. Our findings demonstrate that several cell distribution patterns differ between CYNO and humans. First, the CD4(+):CD8(+) T-cell ratio is lower in CYNO compared with humans. Second, the peripheral blood of CYNO contains a population of CD4(+)CD8(+) T cells. Third, the CD31 level was elevated in all organs studied, suggesting that CD31 may not be an accurate marker of recent thymic emigrants within the CD4(+) T cells of CYNO. Finally the B-cell population is lower in CYNO compared with humans. In summary, although the majority of immune cell populations are similar between cynomolgus macaques and humans, several important differences should be considered when using CYNO in immunologic studies. Our current findings provide valuable information to not only researchers but also veterinarians working with CYNO at research centers, in zoos, or in the wild.
authors.affiliation: Columbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA.
authors.forename: Jonah S
authors.initials: JS
authors.lastname: Zitsman
authors.affiliation: Columbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA.
authors.forename: Paula
authors.initials: P
authors.lastname: Alonso-Guallart
authors.affiliation: Columbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Ovanez
authors.affiliation: Columbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA.
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Columbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA.
authors.forename: Joanna F
authors.initials: JF
authors.lastname: Rosen
authors.affiliation: Columbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA.
authors.forename: Joshua I
authors.initials: JI
authors.lastname: Weiner
authors.affiliation: Columbia Center for Translational Immunology, Columbia University Medical Center, New York, New York, USA; Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Department of Pathobiology and University Laboratory Animal Resources, University of Pennsylvania, Philadelphia, Pennsylvania, USA. rduranst@gmail.com.
authors.forename: Raimon
authors.initials: R
authors.lastname: Duran-Struuck
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology; D016516:CD4-CD8 Ratio; D019002:CD56 Antigen / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006413:Hematopoietic System / Q000166:cytology / Q000276:immunology; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D007962:Leukocytes / Q000145:classification* / Q000166:cytology / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D008252:Macaca fascicularis / Q000033:anatomy & histology* / Q000097:blood / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000166:cytology; D009928:Organ Specificity; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D013045:Species Specificity; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology
chemical_list: D018952:Antigens, CD34; D019002:CD56 Antigen; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D017493:Leukocyte Common Antigens