title: The Transcriptional Response to DNA-Double-Strand Breaks in Physcomitrella patens.
abstract: The model bryophyte Physcomitrella patens is unique among plants in supporting the generation of mutant alleles by facile homologous recombination-mediated gene targeting (GT). Reasoning that targeted transgene integration occurs through the capture of transforming DNA by the homology-dependent pathway for DNA double-strand break (DNA-DSB) repair, we analysed the genome-wide transcriptomic response to bleomycin-induced DNA damage and generated mutants in candidate DNA repair genes. Massively parallel (Illumina) cDNA sequencing identified potential participants in gene targeting. Transcripts encoding DNA repair proteins active in multiple repair pathways were significantly up-regulated. These included Rad51, CtIP, DNA ligase 1, Replication protein A and ATR in homology-dependent repair, Xrcc4, DNA ligase 4, Ku70 and Ku80 in non-homologous end-joining and Rad1, Tebichi/polymerase theta, PARP in microhomology-mediated end-joining. Differentially regulated cell-cycle components included up-regulated Rad9 and Hus1 DNA-damage-related checkpoint proteins and down-regulated D-type cyclins and B-type CDKs, commensurate with the imposition of a checkpoint at G2 of the cell cycle characteristic of homology-dependent DNA-DSB repair. Candidate genes, including ATP-dependent chromatin remodelling helicases associated with repair and recombination, were knocked out and analysed for growth defects, hypersensitivity to DNA damage and reduced GT efficiency. Targeted knockout of PpCtIP, a cell-cycle activated mediator of homology-dependent DSB resection, resulted in bleomycin-hypersensitivity and greatly reduced GT efficiency.
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, Leeds University, Leeds LS2 9JT, United Kingdom.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Kamisugi
authors.affiliation: Bioinformatics Research Group, School of Molecular and Cellular Biology, Leeds University, Leeds LS2 9JT, United Kingdom.
authors.forename: John W
authors.initials: JW
authors.lastname: Whitaker
authors.affiliation: Centre for Plant Sciences, Faculty of Biological Sciences, Leeds University, Leeds LS2 9JT, United Kingdom.
authors.forename: Andrew C
authors.identifier: http://orcid.org/0000-0003-2562-2052
authors.initials: AC
authors.lastname: Cuming
mesh_terms: D001761:Bleomycin / Q000494:pharmacology; D019068:Bryopsida / Q000235:genetics* / Q000502:physiology; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000235:genetics; D020869:Gene Expression Profiling; D055786:Gene Knockout Techniques; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: D001761:Bleomycin