title: Sublingual flagellin protects against acute pneumococcal pneumonia in a TLR5-dependent and NLRC4-independent fashion.
abstract: To evaluate efficacy of sublingual flagellin to treat acute pneumonia.
  
  
  
  Mice were treated sublingually with flagellin and challenged intranasally with a lethal dose of pneumococcus. Flagellins lacking TLR5 or NLRC4 activation domains were used to assess their contribution to protection.
  
  
  
  Sublingual flagellin protected mice in a TLR5-dependent, NLRC4-independent fashion. Neutrophils were required for protection. Flagellin-stimulated lung epithelial cells recapitulated the lung's transcriptional profile suggesting they could be targeted by flagellin in vivo.
  
  
  
  Ligation of TLR5, a pathogen recognition receptor not naturally engaged by pneumococcus, protects mice from invasive pneumonia when administered via sublingual route. This can be a highly cost-effective alternative therapy against pneumonia.
authors.affiliation: Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina-Universidad de la República (UdelaR), Montevideo, 11600, Uruguay.|Adjuvant Research Group, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, D02 PN40, Ireland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Muñoz-Wolf
authors.affiliation: Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina-Universidad de la República (UdelaR), Montevideo, 11600, Uruguay.
authors.forename: Analía
authors.initials: A
authors.lastname: Rial
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR8204 - CIIL - Center for Infection & Immunity of Lille, F-59000 Lille, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Fougeron
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR8204 - CIIL - Center for Infection & Immunity of Lille, F-59000 Lille, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Tabareau
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR8204 - CIIL - Center for Infection & Immunity of Lille, F-59000 Lille, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Sirard
authors.affiliation: Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina-Universidad de la República (UdelaR), Montevideo, 11600, Uruguay.
authors.forename: José A
authors.initials: JA
authors.lastname: Chabalgoity
mesh_terms: D000286:Administration, Sublingual; D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D053468:CARD Signaling Adaptor Proteins / Q000235:genetics / Q000276:immunology*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000276:immunology*; D005260:Female; D005408:Flagellin / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008168:Lung / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology; D011018:Pneumonia, Pneumococcal / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control*; D000072417:Protein Domains; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000276:immunology*; D051198:Toll-Like Receptor 5 / Q000235:genetics / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D053468:CARD Signaling Adaptor Proteins; D002135:Calcium-Binding Proteins; C435692:NLRC4 protein, human; C495346:TLR5 protein, human; D051198:Toll-Like Receptor 5; D005408:Flagellin
keywords: Streptococcus pneumoniae; TLR5; antimicrobial; flagellin; immunotherapy; neutrophils; pneumonia; sublingual