title: BAR Domain-Containing FAM92 Proteins Interact with Chibby1 To Facilitate Ciliogenesis.
abstract: Chibby1 (Cby1) is a small, conserved coiled-coil protein that localizes to centrioles/basal bodies and plays a crucial role in the formation and function of cilia. During early stages of ciliogenesis, Cby1 is required for the efficient recruitment of small vesicles at the distal end of centrioles to facilitate basal body docking to the plasma membrane. Here, we identified family with sequence similarity 92, member A (FAM92A) and FAM92B, which harbor predicted lipid-binding BAR domains, as novel Cby1-interacting partners using tandem affinity purification and mass spectrometry. We found that in cultured cell lines, FAM92A colocalizes with Cby1 at the centrioles/basal bodies of primary cilia, while FAM92B is undetectable. In airway multiciliated cells, both FAM92A and -92B colocalize with Cby1 at the base of cilia. Notably, the centriolar localization of FAM92A and -92B depends largely on Cby1. Knockdown of FAM92A in RPE1 cells impairs ciliogenesis. Consistent with the membrane-remodeling properties of BAR domains, FAM92A and -92B in cooperation with Cby1 induce deformed membrane-like structures containing the small GTPase Rab8 in cultured cells. Our results therefore suggest that FAM92 proteins interact with Cby1 to promote ciliogenesis via regulation of membrane-remodeling processes.
authors.affiliation: Graduate Program in Molecular and Cellular Pharmacology, Stony Brook University, Stony Brook, New York, USA Department of Pharmacological Sciences, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Feng-Qian
authors.initials: FQ
authors.lastname: Li
authors.affiliation: Graduate Program in Molecular and Cellular Pharmacology, Stony Brook University, Stony Brook, New York, USA Department of Pharmacological Sciences, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Xingwang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.
authors.forename: Cody
authors.initials: C
authors.lastname: Fisher
authors.affiliation: Graduate Program in Molecular and Cellular Pharmacology, Stony Brook University, Stony Brook, New York, USA Department of Pharmacological Sciences, Stony Brook University, Stony Brook, New York, USA Medical Scientist Training Program, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Saul S
authors.initials: SS
authors.lastname: Siller
authors.affiliation: Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.
authors.forename: Klara
authors.initials: K
authors.lastname: Zelikman
authors.affiliation: Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Kuriyama
authors.affiliation: Graduate Program in Molecular and Cellular Pharmacology, Stony Brook University, Stony Brook, New York, USA Department of Pharmacological Sciences, Stony Brook University, Stony Brook, New York, USA Medical Scientist Training Program, Stony Brook University, Stony Brook, New York, USA ken-ichi.takemaru@stonybrook.edu.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Takemaru
mesh_terms: D000818:Animals; D064652:Basal Bodies / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002502:Centrioles / Q000378:metabolism; D002923:Cilia / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009024:Morphogenesis; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D000072417:Protein Domains; D055503:Protein Multimerization; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C473750:CBY1 protein, human; C522980:CIBAR1 protein, human; C572790:CIBAR2 protein, human; D002352:Carrier Proteins; C517894:Chibby protein, mouse; D009687:Nuclear Proteins; D011506:Proteins