title: Acute resistance exercise-induced IGF1 expression and subsequent GLUT4 translocation.
abstract: Acute aerobic exercise (AE) is a major physiological stimulus for skeletal muscle glucose uptake through activation of 5' AMP-activated protein kinase (AMPK). However, the regulation of glucose uptake by acute resistance exercise (RE) remains unclear. To investigate the intracellular regulation of glucose uptake after acute RE versus acute AE, male Sprague-Dawley rats were divided into three groups: RE, AE, or nonexercise control. After fasting for 12 h overnight, the right gastrocnemius muscle in the RE group was exercised at maximum isometric contraction via percutaneous electrical stimulation (3 × 10 sec, 5 sets). The AE group ran on a treadmill (25 m/min, 60 min). Muscle samples were taken 0, 1, and 3 h after completion of the exercises. AMPK, Ca(2+)/calmodulin-dependent protein kinase II, and TBC1D1 phosphorylation were increased immediately after both forms of exercise and returned to baseline levels by 3 h. Muscle IGF1 expression was increased by RE but not AE, and maintained until 3 h after RE Additionally, Akt and AS160 phosphorylation were sustained for 3 h after RE, whereas they returned to baseline levels by 3 h after AE Similarly, GLUT4 translocation remained elevated 3 h after RE, although it returned to the baseline level by 3 h after AE Overall, this study showed that AMPK/TBC1D1 and IGF1/Akt/AS160 signaling were enhanced by acute RE, and that GLUT4 translocation after acute RE was more prolonged than after acute AE These results suggest that acute RE-induced increases in intramuscular IGF1 expression might be a distinct regulator of GLUT4 translocation.
authors.affiliation: Faculty of Sport and Health Science, Ritsumeikan University, Kusatsu, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Kido
authors.affiliation: Faculty of Sport and Health Science, Ritsumeikan University, Kusatsu, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Ato
authors.affiliation: Laboratory of Sports and Exercise Medicine, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Yokokawa
authors.affiliation: Department of Physical Education, National Defense Academy, Yokosuka, Japan.
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Makanae
authors.affiliation: Graduate School of Human Development and Environment, Kobe University, Kobe, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Sato
authors.affiliation: Faculty of Sport and Health Science, Ritsumeikan University, Kusatsu, Japan safujita@fc.ritsumei.ac.jp.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fujita
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000015:Abnormalities, Multiple / Q000378:metabolism*; D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000378:metabolism; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000235:genetics*; D006130:Growth Disorders / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D010766:Phosphorylation / Q000235:genetics / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000009:adverse effects / Q000502:physiology*; D000069284:Protein Translocation Systems / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000502:physiology; D004561:Transcutaneous Electric Nerve Stimulation / Q000379:methods
chemical_list: D020690:GTPase-Activating Proteins; D051275:Glucose Transporter Type 4; D000069284:Protein Translocation Systems; D011506:Proteins; C540572:TBC1D1 protein, rat; C533101:TBC1D4 protein, rat; C548904:insulin-like growth factor-1, rat; D007334:Insulin-Like Growth Factor I; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D055372:AMP-Activated Protein Kinases; D005947:Glucose
keywords: Aerobic exercise; glucose transporter type 4; insulin‐like growth factor 1; resistance exercise