title: Effect of intraperitoneal cetuximab administration on colonic anastomosis and early postoperative adhesion formation in a rat model.
abstract: OBJECTIVE
  We aimed to evaluate the effect of intraperitoneal cetuximab administration on the healing of anastomosis and development of early adhesion formation in a rat model.
  
  
  METHODS
  Twenty-four female rats were used. A colon segment was resected and end-to-end anastomosis was performed. The rats were randomized into three groups after the performance of colonic anastomosis and received 10 mL of intraperitoneal solution including study drugs after closure of abdominal cavity: normal saline was administered to the normal saline group (n=8), cetuximab (400 mg/m(2)) was administered to the postoperative 1 group (n=8) 1 day after surgery, and cetuximab (400 mg/m(2)) was administered to the peroperative group (n=8) during surgery.
  
  
  RESULTS
  The mean adhesion grade was 2.63±0.92, and 0.50±0.76 and 0.63±0.74 for control and test groups, respectively. Cetuximab reduced adhesion formation in test groups (p<0.05). When all groups were compared, it was found that vascular endothelial growth factor levels decreased significantly only in the abdomen (p<0.05). Hydroxyproline levels and anastomosis bursting pressure were examined, and a statistical difference was found between groups (hydroxyproline p<0.05, bursting pressure p<0.05). However, when postoperative 1 day group was compared with the control group, it was found that there was no difference between groups according to these parameters (p>0.05), but when peroperative group was compared with the control group a significant decrease was observed in both parameters. Histopathological healing score was also evaluated. No statistical difference between groups was found.
  
  
  CONCLUSIONS
  Twenty-four hours later from the operation, intraperitoneal cetuximab therapy may be a safe and feasible treatment for metastatic colorectal patients.
authors.affiliation: Department of General Surgery, Cumhuriyet University School of Medicine, Sivas, Turkey.
authors.forename: Atilla
authors.initials: A
authors.lastname: Kurt
authors.affiliation: Institude of Oncology, Surgical Oncology Unit, İstanbul University, İstanbul School of Medicine, İstanbul, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Karanlık
authors.affiliation: Department of General Surgery, Cumhuriyet University School of Medicine, Sivas, Turkey.
authors.forename: Sinan
authors.initials: S
authors.lastname: Soylu
authors.affiliation: Institude of Oncology, Surgical Oncology Unit, İstanbul University, İstanbul School of Medicine, İstanbul, Turkey.
authors.forename: İlker
authors.initials: İ
authors.lastname: Özgür
authors.affiliation: Department of Biochemistry, İstanbul University, İstanbul School of Medicine, İstanbul, Turkey.
authors.forename: Hilal Oğuz
authors.initials: HO
authors.lastname: Soydinç
authors.affiliation: Department of Biochemistry, İstanbul University, İstanbul School of Medicine, İstanbul, Turkey.
authors.forename: Derya
authors.initials: D
authors.lastname: Duranyıldız
authors.affiliation: Department of Pathology, İstanbul University, İstanbul School of Medicine, İstanbul, Turkey.
authors.forename: Vakur
authors.initials: V
authors.lastname: Olgaç
authors.affiliation: Department of Medical Oncology, İstanbul University İstanbul School of Medicine, İstanbul, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Şen
authors.affiliation: Department of General Surgery, İstanbul Liv Hospital, İstanbul, Turkey.
authors.forename: Oktar
authors.initials: O
authors.lastname: Asoğlu
keywords: Intraperitoneal chemotherapy; adhesion formation; anastomosis healing; cetuximab