title: Scalable Production of Glioblastoma Tumor-initiating Cells in 3 Dimension Thermoreversible Hydrogels.
abstract: There is growing interest in developing drugs that specifically target glioblastoma tumor-initiating cells (TICs). Current cell culture methods, however, cannot cost-effectively produce the large numbers of glioblastoma TICs required for drug discovery and development. In this paper we report a new method that encapsulates patient-derived primary glioblastoma TICs and grows them in 3 dimension thermoreversible hydrogels. Our method allows long-term culture (~50 days, 10 passages tested, accumulative ~>10(10)-fold expansion) with both high growth rate (~20-fold expansion/7 days) and high volumetric yield (~2.0 × 10(7) cells/ml) without the loss of stemness. The scalable method can be used to produce sufficient, affordable glioblastoma TICs for drug discovery.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, Nebraska, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, Nebraska, USA.
authors.forename: Haishuang
authors.initials: H
authors.lastname: Lin
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, Nebraska, USA.
authors.forename: Ou
authors.initials: O
authors.lastname: Wang
authors.affiliation: Department of Bioengineering, University of California, Los Angeles, Los Angeles, California, USA.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, Nebraska, USA.|Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska, USA.|Fred &Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska, USA.|Nebraska Center for Materials and Nanoscience, University of Nebraska, Lincoln, Nebraska, USA.|Nebraska Center for the Prevention of Obesity Diseases, University of Nebraska, Lincoln, Nebraska, USA.
authors.forename: Srivatsan
authors.initials: S
authors.lastname: Kidambi
authors.affiliation: Department of Neurosurgery, University of Florida College of Medicine, McKnight Brain Institute, Gainesville, Florida, USA.
authors.forename: Loic P
authors.initials: LP
authors.lastname: Deleyrolle
authors.affiliation: Department of Neurosurgery, University of Florida College of Medicine, McKnight Brain Institute, Gainesville, Florida, USA.
authors.forename: Brent A
authors.initials: BA
authors.lastname: Reynolds
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, Nebraska, USA.|Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, Nebraska, USA.|Fred &Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska, USA.
authors.forename: Yuguo
authors.initials: Y
authors.lastname: Lei
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism*; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D020100:Hydrogels / Q000494:pharmacology*; D051379:Mice; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000637:transplantation; D014407:Tumor Cells, Cultured
chemical_list: D020100:Hydrogels