title: Assessment transcallosal Diaschisis in a model of focal cerebral ischemia in rats.
abstract: OBJECTIVE
  To evaluate transcallosal changes after a local ischemic injury in rats by using the monoclonal marker anti-NeuN (Mouse anti-neuronal nuclei).
  
  
  METHODS
  Twenty-eight adult, male, Wistar rats were subjected to focal injury in the right hemisphere. The technique used was the experimental model of focal ischemic injury through intraluminal suture of the middle cerebral artery. Analyses were made for the five groups: after the lesion (control), at 24 h, 96 h, 10 days and 20 days. Exofocal neuronal damage was inferred from neuronal immunoreactivity changes to NeuN.
  
  
  RESULTS
  In the cortex contralateral to the lesion, immunoreactivity was diminished. This finding was most notable in the supra-granular sheets 24 h post ischemia. After 96 h, there was a generalized diminishment of the inmmunoreactivity in the supra and infra-granular sheets. At 10 and 20 days, the tissue recovered some immunoreactivity to NeuN, but there were some changes in the VI layer.
  
  
  CONCLUSIONS
  The immunoreactive changes to NeuN support the process of inter-hemispheric diaschisis. Changes in immunoreactivity could indicate metabolic stress secondary to the disruption in connectivity to the site of lesion.
authors.affiliation: Fundación Valle del Lili, Cali, Colombia; Grupo de Investigación Biomédica, Universidad ICESI, Cali, Colombia.
authors.forename: César Augusto
authors.initials: CA
authors.lastname: Arango-Dávila
authors.affiliation: Fundación Valle del Lili, Cali, Colombia; Grupo Medición y Evaluación Psicológica, Pontificia Universidad Javeriana, Cali, Colombia.
authors.forename: Beatriz Elena
authors.initials: BE
authors.lastname: Muñoz Ospina
authors.affiliation: Grupo Medición y Evaluación Psicológica, Pontificia Universidad Javeriana, Cali, Colombia.
authors.forename: Daniel Manrique
authors.initials: DM
authors.lastname: Castaño
authors.affiliation: Fundación Valle del Lili, Cali, Colombia; Grupo de Investigación Biomédica, Universidad ICESI, Cali, Colombia.
authors.forename: Laura
authors.initials: L
authors.lastname: Potes
authors.affiliation: Centro de Investigación, Innovación y Desarrollo, CIID. Fundación Innovación y Ciencia por Colombia. FICC, Bogotá, Colombia.
authors.forename: John
authors.initials: J
authors.lastname: Umbarila Prieto
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear / Q000032:analysis* / Q000276:immunology; D015415:Biomarkers; D002545:Brain Ischemia / Q000150:complications* / Q000473:pathology; D003337:Corpus Callosum / Q000276:immunology / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D020768:Middle Cerebral Artery; D009336:Necrosis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000911:Antibodies, Monoclonal; D034961:Antigens, Nuclear; D015415:Biomarkers
keywords: Diaschisis; apoptosis; brain Injuries; brain ischemia; immunohistochemistry; physiological stress