title: Purification and characterization of a Cys-Gly hydrolase from the gastropod mollusk, Patella caerulea.
abstract: A magnesium-dependent cysteinyl-glycine hydrolyzing enzyme from the gastropod mollusk Patella caerulea was purified to electrophoretic homogeneity through a simple and rapid purification protocol. The molecular masses of the native protein and the subunit suggest that the enzyme has a homohexameric structure. Structural data in combination with kinetic parameters determined with Cys-Gly and compared with Leu-Gly as a substrate, indicate that the purified enzyme is a member of the peptidase family M17. The finding that an enzyme of the peptidase family M17 is responsible also in mollusks for the breakdown of Cys-Gly confirms the important role of this peptidase family in the glutathione metabolism.
authors.affiliation: a Biology-Biochemistry Unit, University of Pisa , Pisa , Italy and.
authors.forename: Mario
authors.initials: M
authors.lastname: Cappiello
authors.affiliation: a Biology-Biochemistry Unit, University of Pisa , Pisa , Italy and.
authors.forename: Matteo
authors.initials: M
authors.lastname: Spinelli
authors.affiliation: a Biology-Biochemistry Unit, University of Pisa , Pisa , Italy and.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Mormino
authors.affiliation: b ISPAAM-Proteomics & Mass Spectrometry Laboratory, National Research Council , Naples , Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Renzone
authors.affiliation: b ISPAAM-Proteomics & Mass Spectrometry Laboratory, National Research Council , Naples , Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Scaloni
authors.affiliation: a Biology-Biochemistry Unit, University of Pisa , Pisa , Italy and.
authors.forename: Roberta
authors.initials: R
authors.lastname: Moschini
authors.affiliation: a Biology-Biochemistry Unit, University of Pisa , Pisa , Italy and.
authors.forename: Francesco
authors.initials: F
authors.lastname: Balestri
authors.affiliation: a Biology-Biochemistry Unit, University of Pisa , Pisa , Italy and.
authors.forename: Umberto
authors.initials: U
authors.lastname: Mura
authors.affiliation: a Biology-Biochemistry Unit, University of Pisa , Pisa , Italy and.
authors.forename: Antonella
authors.initials: A
authors.lastname: Del-Corso
mesh_terms: D000818:Animals; D003545:Cysteine / Q000737:chemistry*; D005998:Glycine / Q000737:chemistry*; D006867:Hydrolases / Q000378:metabolism*; D008974:Mollusca
chemical_list: D006867:Hydrolases; D003545:Cysteine; D005998:Glycine
keywords: Glutathione metabolism; leucyl aminopeptidase inhibition; parasitic infections; peptidase family M17