title: Characterization of histone modifications associated with DNA damage repair genes upon exposure to gamma rays in Arabidopsis seedlings.
abstract: Dynamic histone modifications play an important role in controlling gene expression in response to various environmental cues. This mechanism of regulation of gene expression is important for sessile organisms, like land plants. We have previously reported consistent upregulation of various marker genes in response to gamma rays at various post-irradiation times. In the present study, we performed various chromatin modification analyses at selected loci using the standard chromatin immunoprecipitation procedure, and demonstrate that upregulation of these genes is associated with histone H3 lysine 4 tri-methylation (H3K4me3) at the gene body or transcription start sites of these loci. Further, at specific AtAgo2 loci, both H3K4me3 and histone H3 lysine 9 acetylation (H3K9ac) are important in controlling gene expression in response to gamma irradiation. There was no change in DNA methylation in these selected loci. We conclude that specific histone modification such as H3K4me3 and H3K9ac may be more important in activating gene expression in these selected loci in response to gamma irradiation than a change in DNA methylation.
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea.|Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors.forename: Suvendu
authors.initials: S
authors.lastname: Mondal
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea.
authors.forename: Young Sam
authors.initials: YS
authors.lastname: Go
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea.
authors.forename: Seung Sik
authors.initials: SS
authors.lastname: Lee
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea jhongkim@kaeri.re.kr jhongkim71@gmail.com.
authors.forename: Byung Yeoup
authors.initials: BY
authors.lastname: Chung
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea jhongkim@kaeri.re.kr jhongkim71@gmail.com.|Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Kim
mesh_terms: D017360:Arabidopsis / Q000528:radiation effects*; D002843:Chromatin / Q000737:chemistry; D047369:Chromatin Immunoprecipitation; D004249:DNA Damage; D019175:DNA Methylation; D004260:DNA Repair; D044127:Epigenesis, Genetic; D005720:Gamma Rays; D020869:Gene Expression Profiling; D018745:Genome, Plant; D059014:High-Throughput Nucleotide Sequencing; D006657:Histones / Q000737:chemistry*; D012313:RNA / Q000032:analysis; D036226:Seedlings / Q000528:radiation effects*
chemical_list: D002843:Chromatin; D006657:Histones; D012313:RNA
keywords: DNA repair genes; chromatin immunoprecipitation; epigenetics; gamma rays; histone modification