title: Fructose during pregnancy provokes fetal oxidative stress: The key role of the placental heme oxygenase-1.
abstract: One of the features of metabolic syndrome caused by liquid fructose intake is an impairment of redox status. We have investigated whether maternal fructose ingestion modifies the redox status in pregnant rats and their fetuses.
  
  
  
  Fructose (10% wt/vol) in the drinking water of rats throughout gestation, leads to maternal hepatic oxidative stress. However, this change was also observed in glucose-fed rats and, in fact, both carbohydrates produced a decrease in antioxidant enzyme activity. Surprisingly, mothers fed carbohydrates displayed low plasma lipid oxidation. In contrast, fetuses from fructose-fed mothers showed elevated levels of plasma lipoperoxides versus fetuses from control or glucose-fed mothers. Interestingly, a clearly augmented oxidative stress was observed in placenta of fructose-fed mothers, accompanied by a lower expression of the transcription factor Nuclear factor-erythroid 2-related factor-2 (Nrf2) and its target gene, heme oxygenase-1 (HO-1), a potent antioxidant molecule. Moreover, histone deacetylase 3 (HDAC3) that has been proposed to upregulate HO-1 expression by stabilizing Nrf2, exhibited a diminished expression in placenta of fructose-supplemented mothers.
  
  
  
  Maternal fructose intake provoked an imbalanced redox status in placenta and a clear diminution of HO-1 expression, which could be responsible for the augmented oxidative stress found in their fetuses.
authors.affiliation: Facultad de Farmacia, Universidad San Pablo-CEU, Madrid, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Rodrigo
authors.affiliation: Facultad de Farmacia, Universidad San Pablo-CEU, Madrid, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Rodríguez
authors.affiliation: Facultad de Farmacia, Universidad San Pablo-CEU, Madrid, Spain.
authors.forename: Paola
authors.initials: P
authors.lastname: Otero
authors.affiliation: Facultad de Farmacia, Universidad San Pablo-CEU, Madrid, Spain.
authors.forename: María I
authors.initials: MI
authors.lastname: Panadero
authors.affiliation: Centre of Metabolomics and Bioanalysis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, Madrid, Spain.
authors.forename: Antonia
authors.initials: A
authors.lastname: García
authors.affiliation: Centre of Metabolomics and Bioanalysis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, Madrid, Spain.
authors.forename: Coral
authors.initials: C
authors.lastname: Barbas
authors.affiliation: Facultad de Farmacia, Universidad de Barcelona, CIBERobn, IBUB, Barcelona, Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Roglans
authors.affiliation: Departamento de Metabolismo y Nutrición, Instituto de Ciencia y Tecnología de Alimentos y Nutrición (ICTAN/CSIC), Madrid, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Ramos
authors.affiliation: Departamento de Metabolismo y Nutrición, Instituto de Ciencia y Tecnología de Alimentos y Nutrición (ICTAN/CSIC), Madrid, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Goya
authors.affiliation: Facultad de Farmacia, Universidad de Barcelona, CIBERobn, IBUB, Barcelona, Spain.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Laguna
authors.affiliation: CQS Lab, Madrid, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Álvarez-Millán
authors.affiliation: Facultad de Farmacia, Universidad San Pablo-CEU, Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Bocos
mesh_terms: D000818:Animals; D005260:Female; D005333:Fetus / Q000187:drug effects / Q000378:metabolism; D005632:Fructose / Q000008:administration & dosage / Q000009:adverse effects*; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism*; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D018811:Maternal Exposure / Q000009:adverse effects*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress; D010920:Placenta / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071717:X-Box Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D000099186:Histone Deacetylase 3
chemical_list: D005632:Fructose; D006419:Heme Oxygenase (Decyclizing); D006655:Histone Deacetylases; D016660:NAD(P)H Dehydrogenase (Quinone); D051267:NF-E2-Related Factor 2; D000071717:X-Box Binding Protein 1; D000099186:Histone Deacetylase 3; C495637:Nfe2l2 protein, rat; C000605545:Xbp1 protein, rat; C491523:Hmox1 protein, rat
keywords: Fructose; Heme oxygenase-1; Oxidative stress; Placenta; Pregnancy