title: miR-200 Regulates Endometrial Development During Early Pregnancy.
abstract: For successful embryo implantation, endometrial stromal cells must undergo functional and morphological changes, referred to as decidualization. However, the molecular mechanisms that regulate implantation and decidualization are not well defined. Here we demonstrate that the estradiol- and progesterone-regulated microRNA (miR)-200 family was markedly down-regulated in mouse endometrial stromal cells prior to implantation, whereas zinc finger E-box binding homeobox-1 and -2 and other known and predicted targets were up-regulated. Conversely, miR-200 was up-regulated during in vitro decidualization of human endometrial stromal cells. Knockdown of miR-200 negatively affected decidualization and prevented the mesenchymal-epithelial transition-like changes that accompanied decidual differentiation. Notably, superovulation of mice and humans altered miR-200 expression. Our findings suggest that hormonal alterations that accompany superovulation may negatively impact endometrial development and decidualization by causing aberrant miR-200 expression.
authors.affiliation: Departments of Obstetrics and Gynecology (P.T.J., R.A.W., W.L.K., C.R.M.) and Biochemistry (C.R.M.) and Green Center for Reproductive Biology Sciences (P.T.J., R.A.W., W.L.K., C.R.M.), University of Texas Southwestern Medical Center, Dallas, Texas 75390; and Department of Obstetrics and Gynecology (M.A.M.), University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104.
authors.forename: Patricia T
authors.initials: PT
authors.lastname: Jimenez
authors.affiliation: Departments of Obstetrics and Gynecology (P.T.J., R.A.W., W.L.K., C.R.M.) and Biochemistry (C.R.M.) and Green Center for Reproductive Biology Sciences (P.T.J., R.A.W., W.L.K., C.R.M.), University of Texas Southwestern Medical Center, Dallas, Texas 75390; and Department of Obstetrics and Gynecology (M.A.M.), University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104.
authors.forename: Monica A
authors.initials: MA
authors.lastname: Mainigi
authors.affiliation: Departments of Obstetrics and Gynecology (P.T.J., R.A.W., W.L.K., C.R.M.) and Biochemistry (C.R.M.) and Green Center for Reproductive Biology Sciences (P.T.J., R.A.W., W.L.K., C.R.M.), University of Texas Southwestern Medical Center, Dallas, Texas 75390; and Department of Obstetrics and Gynecology (M.A.M.), University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104.
authors.forename: R Ann
authors.initials: RA
authors.lastname: Word
authors.affiliation: Departments of Obstetrics and Gynecology (P.T.J., R.A.W., W.L.K., C.R.M.) and Biochemistry (C.R.M.) and Green Center for Reproductive Biology Sciences (P.T.J., R.A.W., W.L.K., C.R.M.), University of Texas Southwestern Medical Center, Dallas, Texas 75390; and Department of Obstetrics and Gynecology (M.A.M.), University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104.
authors.forename: W Lee
authors.initials: WL
authors.lastname: Kraus
authors.affiliation: Departments of Obstetrics and Gynecology (P.T.J., R.A.W., W.L.K., C.R.M.) and Biochemistry (C.R.M.) and Green Center for Reproductive Biology Sciences (P.T.J., R.A.W., W.L.K., C.R.M.), University of Texas Southwestern Medical Center, Dallas, Texas 75390; and Department of Obstetrics and Gynecology (M.A.M.), University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104.
authors.forename: Carole R
authors.initials: CR
authors.lastname: Mendelson
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D014945:Wound Healing / Q000235:genetics / Q000502:physiology
chemical_list: D035683:MicroRNAs; C543719:Mirn200 microRNA, mouse