title: Synthesis of Novel Pyrimethanil Grafted Chitosan Derivatives with Enhanced Antifungal Activity.
abstract: In this study, three pyrimethanil grafted chitosan (PML-g-CS) derivatives were obtained. The structures of the conjugates were confirmed by FT-IR, (1)H NMR, and EA. The grafting ratios were measured by HPLC. Antifungal properties of pyrimethanil grafted chitosan (PML-g-CS) derivatives against the plant pathogenic fungi Rhizoctonia solani and Gibberella zeae were investigated at concentrations of 100, 200, and 400 mg/L. The PML-g-CS derivatives showed enhanced antifungal activity in comparison with chitosan. The PML-g-CS-1 showed the best antifungal activity against R. solani, whose antifungal index was 58.32%. The PML-g-CS-2 showed the best antifungal activity against G. zeae, whose antifungal index was 53.48%. The conjugation of chitosan and pyrimethanil showed synergistic effect. The PML-g-CS derivatives we developed showed potential for further study and application in crop protection.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China.
authors.forename: Yan
authors.identifier: 0000-0001-9840-1301
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China.
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China.
authors.forename: Yukun
authors.identifier: 0000-0001-6497-462X
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China.
authors.forename: Ronge
authors.initials: R
authors.lastname: Xing
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China.
authors.forename: Kecheng
authors.identifier: 0000-0002-8755-924X
authors.initials: K
authors.lastname: Li
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China.
authors.forename: Pengcheng
authors.identifier: 0000-0002-8660-4950
authors.initials: P
authors.lastname: Li
mesh_terms: D001681:Biological Assay; D048271:Chitosan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D000069601:Crop Protection; D018556:Crops, Agricultural / Q000382:microbiology; D004305:Dose-Response Relationship, Drug; D005659:Fungicides, Industrial / Q000737:chemistry*; D005874:Gibberella / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D011743:Pyrimidines / Q000737:chemistry*; D012232:Rhizoctonia / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005659:Fungicides, Industrial; D011743:Pyrimidines; C108337:pyrimethanil; D048271:Chitosan