title: New Methods for the Site-Selective Placement of Peptides on a Microelectrode Array: Probing VEGF-v107 Binding as Proof of Concept.
abstract: Cu(I)-catalyzed "click" reactions cannot be performed on a borate ester derived polymer coating on a microelectrode array because the Cu(II) precursor for the catalyst triggers background reactions between both acetylene and azide groups with the polymer surface. Fortunately, the Cu(II)-background reaction can itself be used to site-selectively add the acetylene and azide nucleophiles to the surface of the array. In this way, molecules previously functionalized for use in "click" reactions can be added directly to the array. In a similar fashion, activated esters can be added site-selectively to a borate ester coated array. The new chemistry can be used to explore new biological interactions on the arrays. Specifically, the binding of a v107 derived peptide with both human and murine VEGF was probed using a functionalized microelectrode array.
authors.affiliation: Department of Chemistry, Washington University in St. Louis , St. Louis, Missouri 63130, United States.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Graaf
authors.affiliation: Department of Radiology, Washington University in St. Louis , St. Louis, Missouri 63110, United States.
authors.forename: Bernadette V
authors.initials: BV
authors.lastname: Marquez
authors.affiliation: Department of Chemistry, Washington University in St. Louis , St. Louis, Missouri 63130, United States.
authors.forename: Nai-Hua
authors.initials: NH
authors.lastname: Yeh
authors.affiliation: Department of Chemistry, Washington University in St. Louis , St. Louis, Missouri 63130, United States.|Department of Radiology, Washington University in St. Louis , St. Louis, Missouri 63110, United States.
authors.forename: Suzanne E
authors.initials: SE
authors.lastname: Lapi
authors.affiliation: Department of Chemistry, Washington University in St. Louis , St. Louis, Missouri 63130, United States.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Moeller
mesh_terms: D003300:Copper / Q000737:chemistry; D004951:Esterification; D006801:Humans; D008839:Microelectrodes; D010455:Peptides / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry*
chemical_list: D010455:Peptides; D042461:Vascular Endothelial Growth Factor A; D003300:Copper