title: Evaluation of the Cross-Protective Efficacy of a Chimeric Porcine Reproductive and Respiratory Syndrome Virus Constructed Based on Two Field Strains.
abstract: One of the major hurdles to porcine reproductive and respiratory syndrome (PRRS) vaccinology is the limited or no cross-protection conferred by current vaccines. To overcome this challenge, a PRRS chimeric virus (CV) was constructed using an FL12-based cDNA infectious clone in which open reading frames (ORFs) 3-4 and ORFs 5-6 were replaced with the two Korean field isolates K08-1054 and K07-2273,respectively. This virus was evaluated as a vaccine candidate to provide simultaneous protection against two genetically distinct PRRS virus (PRRSV) strains. Thirty PRRS-negative three-week-old pigs were divided into five groups and vaccinated with CV, K08-1054, K07-2273, VR-2332, or a mock inoculum. At 25 days post-vaccination (dpv), the pigs in each group were divided further into two groups and challenged with either K08-1054 or K07-2273. All of the pigs were observed until 42 dpv and were euthanized for pathological evaluation. Overall, the CV-vaccinated group exhibited higher levels of tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), and interleukin-12 (IL-12) expression and of serum virus-neutralizing antibodies compared with the other groups after vaccination and also demonstrated better protection levels against both viruses compared with the challenge control group. Based on these results, it was concluded that CV might be an effective vaccine model that can confer a broader range of cross-protection to various PRRSV strains.
authors.affiliation: College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea. drnadurose@gmail.com.
authors.forename: Nadeem
authors.initials: N
authors.lastname: Shabir
authors.affiliation: College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea. amina.vet.sau.bd@gmail.com.
authors.forename: Amina
authors.initials: A
authors.lastname: Khatun
authors.affiliation: College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea. saliknazki@jbnu.ac.kr.
authors.forename: Salik
authors.initials: S
authors.lastname: Nazki
authors.affiliation: College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea. bskims@jbnu.ac.kr.
authors.forename: Bumseok
authors.initials: B
authors.lastname: Kim
authors.affiliation: Viral Disease Division, Animal and Plant Quarantine Agency, Anyang 430-757, Korea. choiej@korea.kr.
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. dong.sun@zoetis.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Sun
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. kyoon@iastate.edu.
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Yoon
authors.affiliation: College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea. kwi0621@jbnu.ac.kr.
authors.forename: Won-Il
authors.initials: WI
authors.lastname: Kim
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D056738:Cross Protection; D016207:Cytokines / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology*; D011995:Recombination, Genetic; D013552:Swine; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D016207:Cytokines; D014614:Vaccines, Synthetic; D014765:Viral Vaccines
keywords: chimeric virus; cytokines; pig; porcine reproductive and respiratory syndrome; serum virus neutralizing antibodies; vaccine