title: Effect of a chemical inhibitor of human phosphatidylethanolamine-binding protein 4 on radiosensitivity of rectal cancer cells.
abstract: BACKGROUND
  Human phosphatidylethanolamine-binding protein 4 (hPEBP4) is a well-established antiapoptosis molecule in recent years. It has also been demonstrated to be involved in the radioresistance of rectal cancer. The objective of this study was to determine whether IOI-42, a chemical inhibitor of hPEBP4, could sensitize rectal cancer cells.
  
  
  METHODS
  Rectal cancer cells were treated with IOI-42 alone or in combination with irradiation. Clonogenic survival assays and tumor volume growth analysis were used, respectively, to study the effect of IOI-42 in vitro and in vivo. Western blot was adopted to measure the activation of signal pathway.
  
  
  RESULTS
  Clonogenic survival assays showed that IOI-42, combined with irradiation, caused a significant decrease in colony formation compared with radiation alone, which was associated with the downregulation of Akt activation. And we also confirmed the effect of IOI-42 in nude mice transplanted with human rectal cancer subcutaneously.
  
  
  CONCLUSIONS
  These data suggest that IOI-42 has a potential to enhance the radiosensitivity of rectal cancer cells, providing a rationale to further investigate the feasibility of combining of IOI-42 with radiation, keeping in mind that this may result in unexpected toxicities.
authors.affiliation: Department of Colorectal Surgery, The Third Hospital of Hangzhou, 38 West Lake Avenue, ShangChen District, Hangzhou, China. 15168427344@163.com.
authors.forename: Jianming
authors.identifier: http://orcid.org/0000-0003-2798-5287
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Department of Colorectal Surgery, The Third Hospital of Hangzhou, 38 West Lake Avenue, ShangChen District, Hangzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Department of Colorectal Surgery, The Third Hospital of Hangzhou, 38 West Lake Avenue, ShangChen District, Hangzhou, China.
authors.forename: Guangen
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Colorectal Surgery, The Third Hospital of Hangzhou, 38 West Lake Avenue, ShangChen District, Hangzhou, China.
authors.forename: Kan
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Pathology, The Third Hospital of Hangzhou, 38 West Lake Avenue, ShangChen District, Hangzhou, China.
authors.forename: A Li
authors.initials: AL
authors.lastname: Lin
authors.affiliation: Department of Pathology, The Third Hospital of Hangzhou, 38 West Lake Avenue, ShangChen District, Hangzhou, China.
authors.forename: Liuyu
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D005720:Gamma Rays; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008819:Mice, Nude; D049488:Phosphatidylethanolamine Binding Protein / Q000037:antagonists & inhibitors*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011836:Radiation Tolerance / Q000187:drug effects*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D012004:Rectal Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
chemical_list: C518018:PE-binding protein 4, human; D049488:Phosphatidylethanolamine Binding Protein; D011838:Radiation-Sensitizing Agents; D051057:Proto-Oncogene Proteins c-akt
keywords: Human phosphatidylethanolamine-binding protein 4; Radiosensitivity; Rectal cancer