title: Microarray analyses for identifying genes conferring resistance to pepper leaf curl virus in chilli pepper (Capsicum spp.).
abstract: Pepper leaf curl virus (PepLCV) is a serious threat to pepper (Capsicum spp.) production worldwide. Molecular mechanism underlying pepper plants response to PepLCV infection is key to develop PepLCV resistant varieties. In this study, we generated transcriptome profiles of PepLCV resistant genotype (BS-35) and susceptible genotype (IVPBC-535) after artificial viral inoculation using microarray technology and detail experimental procedures and analyses are described. A total of 319 genes differentially expressed between resistant and susceptible genotypes were identified, out of that 234 unique genes were found to be up-regulated > 2-fold in resistant line BS-35 when compared to susceptible, IVPBC-535. The data set we generated has been analyzed to identify genes that are involved in the regulation of resistance against PepLCV. The raw data have been deposited in the Gene Expression Omnibus (GEO) database under accession number GSE41131.
authors.affiliation: Department of Genetics & Plant Breeding, Banaras Hindu University, Varanasi 221005, India; ICAR-Indian Institute of Vegetable Research, Varanasi 221305, India.
authors.forename: Ved Prakash
authors.initials: VP
authors.lastname: Rai
authors.affiliation: ICAR-Indian Institute of Vegetable Research, Varanasi 221305, India.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Rai
authors.affiliation: ICAR-Indian Institute of Vegetable Research, Varanasi 221305, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department of Genetics & Plant Breeding, Jawaharlal Nehru Krishi Vishwavidyalaya, Jabalpur 482004, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.affiliation: World Vegetable Center, P.O. Box 42, Shanhua, Tainan 741, Taiwan.
authors.forename: Sanjeet
authors.initials: S
authors.lastname: Kumar
authors.affiliation: ICAR-Indian Institute of Vegetable Research, Varanasi 221305, India.
authors.forename: Major
authors.initials: M
authors.lastname: Singh
authors.affiliation: Department of Genetics & Plant Breeding, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Sheo Pratap
authors.initials: SP
authors.lastname: Singh
keywords: Agilent; Begomovirus; Cross species hybridization; Molecular mechanism