title: DNA DAMAGE BINDING PROTEIN2 Shapes the DNA Methylation Landscape.
abstract: In eukaryotes, DNA repair pathways help to maintain genome integrity and epigenomic patterns. However, the factors at the nexus of DNA repair and chromatin modification/remodeling remain poorly characterized. Here, we uncover a previously unrecognized interplay between the DNA repair factor DNA DAMAGE BINDING PROTEIN2 (DDB2) and the DNA methylation machinery inArabidopsis thalianaLoss-of-function mutation in DDB2 leads to genome-wide DNA methylation alterations. Genetic and biochemical evidence indicate that at many repeat loci, DDB2 influences de novo DNA methylation by interacting with ARGONAUTE4 and by controlling the local abundance of 24-nucleotide short interfering RNAs (siRNAs). We also show that DDB2 regulates active DNA demethylation mediated by REPRESSOR OF SILENCING1 and DEMETER LIKE3. Together, these findings reveal a role for the DNA repair factor DDB2 in shaping the Arabidopsis DNA methylation landscape in the absence of applied genotoxic stress.
authors.affiliation: Institut de Biologie Moléculaire de Plantes du CNRS, UPR 2357, F-67084 Strasbourg, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Schalk
authors.affiliation: Ecole Normale Supérieure, PSL Research University, Institut de Biologie de l'Ecole Normale Supérieure, CNRS UMR 8197, INSERM U1024, F-75005 Paris, France.
authors.forename: Stéphanie
authors.identifier: http://orcid.org/0000-0002-2356-7021
authors.initials: S
authors.lastname: Drevensek
authors.affiliation: Ecole Normale Supérieure, PSL Research University, Institut de Biologie de l'Ecole Normale Supérieure, CNRS UMR 8197, INSERM U1024, F-75005 Paris, France.
authors.forename: Amira
authors.initials: A
authors.lastname: Kramdi
authors.affiliation: Ecole Normale Supérieure, PSL Research University, Institut de Biologie de l'Ecole Normale Supérieure, CNRS UMR 8197, INSERM U1024, F-75005 Paris, France.
authors.forename: Mohamed
authors.identifier: http://orcid.org/0000-0002-9913-1398
authors.initials: M
authors.lastname: Kassam
authors.affiliation: Ecole Normale Supérieure, PSL Research University, Institut de Biologie de l'Ecole Normale Supérieure, CNRS UMR 8197, INSERM U1024, F-75005 Paris, France.
authors.forename: Ikhlak
authors.initials: I
authors.lastname: Ahmed
authors.affiliation: Institut de Biologie Moléculaire de Plantes du CNRS, UPR 2357, F-67084 Strasbourg, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Cognat
authors.affiliation: Institut de Biologie Moléculaire de Plantes du CNRS, UPR 2357, F-67084 Strasbourg, France.
authors.forename: Stéfanie
authors.initials: S
authors.lastname: Graindorge
authors.affiliation: Institut de Biologie Moléculaire de Plantes du CNRS, UPR 2357, F-67084 Strasbourg, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Bergdoll
authors.affiliation: Institut de Biologie Moléculaire de Plantes du CNRS, UPR 2357, F-67084 Strasbourg, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Baumberger
authors.affiliation: Institut de Biologie Moléculaire de Plantes du CNRS, UPR 2357, F-67084 Strasbourg, France.
authors.forename: Dimitri
authors.initials: D
authors.lastname: Heintz
authors.affiliation: Ecole Normale Supérieure, PSL Research University, Institut de Biologie de l'Ecole Normale Supérieure, CNRS UMR 8197, INSERM U1024, F-75005 Paris, France.
authors.forename: Chris
authors.initials: C
authors.lastname: Bowler
authors.affiliation: Institut de Biologie Moléculaire de Plantes du CNRS, UPR 2357, F-67084 Strasbourg, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Genschik
authors.affiliation: Ecole Normale Supérieure, PSL Research University, Institut de Biologie de l'Ecole Normale Supérieure, CNRS UMR 8197, INSERM U1024, F-75005 Paris, France.
authors.forename: Fredy
authors.initials: F
authors.lastname: Barneche
authors.affiliation: Ecole Normale Supérieure, PSL Research University, Institut de Biologie de l'Ecole Normale Supérieure, CNRS UMR 8197, INSERM U1024, F-75005 Paris, France.
authors.forename: Vincent
authors.identifier: http://orcid.org/0000-0002-6382-1610
authors.initials: V
authors.lastname: Colot
authors.affiliation: Institut de Biologie Moléculaire de Plantes du CNRS, UPR 2357, F-67084 Strasbourg, France jean.molinier@ibmp-cnrs.unistra.fr.
authors.forename: Jean
authors.initials: J
authors.lastname: Molinier