title: FAT1 is a novel upstream regulator of HIF1α and invasion of high grade glioma.
abstract: The hypoxic microenvironment is an important contributor of glioblastoma (GBM) aggressiveness via HIF1α, while tumour inflammation is profoundly influenced by FAT Atypical Cadherin (FAT1). This study was designed to explore the functional interaction and significance of FAT1 and HIF1α under severe hypoxia-mimicking tumour microenvironment in primary human tumours. We first identified a positive correlation of FAT1 with HIF1α and its target genes in GBM tumour specimens, revealing the significance of the FAT1-HIF1α axis in glioma cells. We found that severe hypoxia leads to an increased expression of FAT1 and HIF1α in U87MG and U373MG cells. To reveal the relevance of FAT1 under hypoxic conditions, we depleted endogenous FAT1 under hypoxia and found a substantial reduction in the expression of HIF1α and its downstream target genes like CA9, GLUT1, VEGFA, MCT4, HK2, BNIP3 and REDD1, as well as a significant reduction in the invasiveness in GBM cells. At the molecular level, under hypoxia the FAT1 depletion-associated reduction in HIF1α was due to compromised EGFR-Akt signaling as well as increased VHL-dependent proteasomal degradation of HIF1α. In brief, for the first time, these results reveal an upstream master regulatory role of FAT1 in the expression and role of HIF1α under hypoxic conditions and that FAT1-HIF1α axis controls the invasiveness of GBM. Hence, FAT1 represents a novel potential therapeutic target for GBM.
authors.affiliation: Department of Biochemistry, AIIMS, New Delhi, India.
authors.forename: Evanka
authors.initials: E
authors.lastname: Madan
authors.affiliation: Department of Biochemistry, AIIMS, New Delhi, India.
authors.forename: Bhawana
authors.initials: B
authors.lastname: Dikshit
authors.affiliation: Department of Biochemistry, AIIMS, New Delhi, India.
authors.forename: Srinivas H
authors.initials: SH
authors.lastname: Gowda
authors.affiliation: Department of Biochemistry, AIIMS, New Delhi, India.
authors.forename: Chitrangda
authors.initials: C
authors.lastname: Srivastava
authors.affiliation: Department of Pathology, AIIMS, New Delhi, India.
authors.forename: Chitra
authors.initials: C
authors.lastname: Sarkar
authors.affiliation: Department of Biochemistry, AIIMS, New Delhi, India.
authors.forename: Parthaprasad
authors.initials: P
authors.lastname: Chattopadhyay
authors.affiliation: Department of Biochemistry, AIIMS, New Delhi, India. kunzangchosdol@yahoo.com.|Director, National Brain Research Center, Manesar, Gurgaon, India. kunzangchosdol@yahoo.com.
authors.forename: Subrata
authors.initials: S
authors.lastname: Sinha
authors.affiliation: Department of Biochemistry, AIIMS, New Delhi, India. kchosdol@aiims.ac.in.
authors.forename: Kunzang
authors.initials: K
authors.lastname: Chosdol
mesh_terms: D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000378:metabolism; D055785:Gene Knockdown Techniques; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis* / Q000235:genetics; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D015398:Signal Transduction; D051794:Von Hippel-Lindau Tumor Suppressor Protein / Q000096:biosynthesis
chemical_list: D015820:Cadherins; C502403:FAT1 protein, human; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051794:Von Hippel-Lindau Tumor Suppressor Protein; C512478:EGFR protein, human; D066246:ErbB Receptors; D051057:Proto-Oncogene Proteins c-akt; C497446:VHL protein, human
keywords: EGFR-AKT; FAT1; Glioblastoma; HIF1α; Hypoxia; VHL