title: An rs13293512 polymorphism in the promoter of let-7 is associated with a reduced risk of ischemic stroke.
abstract: The expression of let-7 family members was differentiated in ischemic stroke (IS), functioning as an important regulating molecular in the pathophysiology of stroke. We hypothesized that genetic polymorphism in the promoters of let-7 family may be associated with the risk of IS. To test this hypothesis, we investigated the association of the rs10877887 and rs13293512 in the promoters of let-7 family with the susceptibility to IS. A hospital-based case-control study was performed. The rs10877887 genotype was determined by using a polymerase chain reaction-restriction fragment length polymorphism assay, and the rs13293512 genotype was determined by using a TaqMan assay. We found that the rs13293512CC genotype was associated with a reduced risk of IS (CC vs. TT: adjusted OR = 0.43, 95 % CI 0.26-0.71; dominant model: adjusted OR = 0.70, 95 % CI 0.49-0.98; recessive model: adjusted OR = 0.45, 95 % CI, 0.28-0.73). Stratification analysis showed that the rs10877887TT carriers had a higher level of total cholesterol compared to rs10877887TC/CC carriers (P = 0.03). Combined analysis showed that the rs10877887TC/CC and rs13293512TC/CC genotypes had a reduced risk of IS risk (adjusted OR = 0.58, 95 % CI 0.36-0.95). Our findings suggest that the rs13293512 polymorphism may be a protective factor for the development of IS.
authors.affiliation: Department of Neurology, The First People's Hospital of Qujing City, Qingjing, 655000, Yunnan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The First People's Hospital of Qujing City, Qingjing, 655000, Yunnan, China.
authors.forename: Junsu
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Yanan Hospital of Kunming, Kunming, 650051, Yunnan, China. xueqiang3513@126.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xue
authors.affiliation: Department of Cardiology, Yanan Hospital of Kunming, Kunming, 650051, Yunnan, China. yd707@163.com.
authors.forename: Dong
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Yanan Hospital of Kunming, Kunming, 650051, Yunnan, China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Cardiology, Yanan Hospital of Kunming, Kunming, 650051, Yunnan, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Guang
authors.affiliation: Department of Cardiology, Yanan Hospital of Kunming, Kunming, 650051, Yunnan, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The First People's Hospital of Qujing City, Qingjing, 655000, Yunnan, China.
authors.forename: Ruiqiong
authors.initials: R
authors.lastname: Ba
authors.affiliation: Department of Neurology, The First People's Hospital of Qujing City, Qingjing, 655000, Yunnan, China.
authors.forename: Hongwen
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Neurology, The First People's Hospital of Qujing City, Qingjing, 655000, Yunnan, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ma
mesh_terms: D000368:Aged; D002545:Brain Ischemia / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D002784:Cholesterol / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D020521:Stroke / Q000097:blood / Q000235:genetics*
chemical_list: D035683:MicroRNAs; C487931:mirnlet7 microRNA, human; D002784:Cholesterol
keywords: Ischemic stroke; Let-7; Polymorphism; Promoter