title: Low-Dose Steroid Therapy Is Associated with Decreased IL-12 Production in PBMCs of Severe Septic Patients.
abstract: Background. Sepsis-induced immunosuppression may result in higher mortality rates in patients. Methods. We examined the relationship of cytokine responses from stimulated peripheral blood mononuclear cells (PBMCs) and monocyte human leukocyte antigen-DR (HLA-DR) expression (days 1 and 7) with low-dose steroid therapy in 29 septic patients. Patients were treated according to the guidelines. Thirty healthy controls were enrolled for validation. Results. Eighteen patients were prescribed low-dose steroids and 11 were not. Interleukin- (IL-) 12 responses in patients without low-dose steroid therapy on days 1 and 7 were higher than those with low-dose steroid therapy. Compared to day 1, IL-12 responses significantly increased on day 7 in patients without low-dose steroid therapy. After regression analysis, the change in the IL-12 response from day 7 to day 1 was found to be independently associated with the low-dose steroid therapy. There was no difference in monocyte HLA-DR expression between patients treated with and without low-dose steroid on day 1 or 7. No change in monocyte HLA-DR expression from day 7 to day 1 was observed in patients with or without low-dose steroid therapy. Conclusion. Decreased IL-12 response was associated with the low-dose steroid therapy in PBMCs of septic patients.
authors.affiliation: Division of Pulmonary, Critical Care and Sleep Medicine, Chang Gung Memorial Hospital, Keelung 204, Taiwan; Chang Gung University College of Medicine, Taoyuan 333, Taiwan.
authors.forename: Huang-Pin
authors.identifier: 0000-0002-8906-4566
authors.initials: HP
authors.lastname: Wu
authors.affiliation: Department of Emergence, Chang Gung Memorial Hospital, Keelung 204, Taiwan.
authors.forename: Chi-Chung
authors.initials: CC
authors.lastname: Shih
authors.affiliation: Department of Chemistry, National Chung-Hsing University, Taichung 402, Taiwan.
authors.forename: Duen-Yau
authors.initials: DY
authors.lastname: Chuang
authors.affiliation: Chang Gung University College of Medicine, Taoyuan 333, Taiwan; Division of Cardiology, Chang Gung Memorial Hospital, Keelung 204, Taiwan.
authors.forename: Tien-Hsing
authors.initials: TH
authors.lastname: Chen
mesh_terms: D000368:Aged; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D007963:Leukocytes, Mononuclear; D016014:Linear Models; D008297:Male; D018123:Receptors, Interleukin / Q000378:metabolism; D012189:Retrospective Studies; D018805:Sepsis / Q000188:drug therapy* / Q000276:immunology* / Q000378:metabolism; D013256:Steroids / Q000008:administration & dosage / Q000627:therapeutic use*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: C448419:IL17RC protein, human; D015850:Interleukin-6; D018123:Receptors, Interleukin; D013256:Steroids; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12