title: Expression patterns of CD56+ and CD16+ cells in renal transplant biopsies with acute rejection: Associations with microcirculation injuries and graft survival.
abstract: BACKGROUND
  The study investigated whether immunohistochemical features of interstitial and glomerular CD56 and CD16 infiltrates - NK cells markers - could be associated with microcirculation injury scores - peritubular capillaritis (ptc) and glomerulitis (g) - and graft survival.
  
  
  METHODS
  The research analyzed the immunohistochemical pattern of CD56 and CD16 in interstitial and glomerular compartments of biopsies for-cause biopsies from 59 recipients diagnosed with acute rejection (mean = 135.5 days post-transplant).
  
  
  RESULTS
  Interstitial CD56+ cells had an increased expression for glomerulitis (g ≥ 1) (P = 0.02) and peritubular capillaritis (ptc ≥ 2) (P = 0.003) presence. It was noted that interstitial CD56 + cells with mean above 0.56 cells/mm2had worse allograft survival. CD56+ cells in the interstitial compartment with mean less than or equal to 0.56cells/mm2was related with absence or mild peritubular capillaritis (P = 0.012) and mean above 0.56 cells/mm2, respectively, with glomerulitis (P = 0.002) presence. Interstitial CD16 cells showed greater positive results in relation to peritubular capillaritis (P = 0.0001) cases. Similarly, it was observed that glomerular CD16+ cells had higher positive results in glomerulitis (P = 0.009) presence.
  
  
  CONCLUSIONS
  The study findings showed that CD56+ cell infiltrated in the interstitial compartment was significantly associated with microcirculation injury scores, especially the glomerulitis, and graft survival.
authors.affiliation: Department of Pathology, College of Medicine, São Paulo State University, Botucatu, São Paulo, Brazil.|Department of Pathology, College of Medicine, University of São Paulo, Brazil.
authors.forename: Daniela Cristina
authors.initials: DC
authors.lastname: Dos Santos
authors.affiliation: Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, Brazil.
authors.forename: Niels Olsen
authors.initials: NO
authors.lastname: Saraiva Camara
authors.affiliation: Department of Pathology, College of Medicine, University of São Paulo, Brazil.
authors.forename: Daisa Silva Ribeiro
authors.initials: DSR
authors.lastname: David
authors.affiliation: Department of Pathology, College of Medicine, University of São Paulo, Brazil.
authors.forename: Denise Maria Avancini Costa
authors.initials: DMAC
authors.lastname: Malheiros
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D001706:Biopsy; D019002:CD56 Antigen / Q000032:analysis*; D005260:Female; D058851:GPI-Linked Proteins / Q000032:analysis; D006084:Graft Rejection; D006085:Graft Survival; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008833:Microcirculation; D008875:Middle Aged; D017452:Receptors, IgG / Q000032:analysis*; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D019002:CD56 Antigen; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; C522918:NCAM1 protein, human; D017452:Receptors, IgG
keywords: Natural Killer cells; immunostaining; microcirculation injury; renal allograft survival; renal pathology; transplant