title: MiR-203 inhibits tumor invasion and metastasis in gastric cancer by ATM.
abstract: Gastric cancer is one of the most common malignancies in the world. A number of miRNAs are aberrantly expressed during the progression of gastric cancer. In this study, we aimed to investigate the role of miR-203 in the invasion and metastasis of gastric cancer and the potential mechanism of the effect of miR-203 on the tumor progression of gastric cancer. Our results showed that miR-203 was significantly downregulated in gastric cancer tissues and cells, while ataxia telangiectasia mutated kinase (ATM) was upregulated in gastric cancer tissues and cells and was directly regulated by miR-203. Ectopic overexpression of miR-203 inhibited the colony formation, migration, and invasion of gastric cancer cells. In addition, miR-203 overexpression significantly suppressed the protein level of Snail and obviously promoted the protein level of E-cadherin in gastric cancer cells. ATM knockdown phenocopied the effect of miR-203 overexpression. These results suggested that miR-203 suppressed the migration and invasion of gastric cancer through regulating the level of ATM-mediated-Snail and E-cadherin. MiR-203 might be a novel therapeutic strategy for the treatment of gastric cancer.
authors.affiliation: Department of Pathology, Qian-fo-shan Hospital Affiliated to Shandong University, Jinan 250014, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Pathology, Qian-fo-shan Hospital Affiliated to Shandong University, Jinan 250014, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Department of Pathology, Qian-fo-shan Hospital Affiliated to Shandong University, Jinan 250014, China.
authors.forename: Guo-Xia
authors.initials: GX
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Qian-fo-shan Hospital Affiliated to Shandong University, Jinan 250014, China sq167660@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
mesh_terms: D064007:Ataxia Telangiectasia Mutated Proteins / Q000502:physiology*; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D000071250:Snail Family Transcription Factors / Q000378:metabolism; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D015820:Cadherins; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; D000071250:Snail Family Transcription Factors; C576324:ATM protein, human; D064007:Ataxia Telangiectasia Mutated Proteins
keywords: ATM; gastric cancer; invasion; miR-203; migration