title: Chemopreventive effect and angiogenic activity of punicalagin isolated from leaves of Lafoensia pacari A. St.-Hil.
abstract: Punicalagin is the major ellagitannin constituent from leaves of Lafoensia pacari, a Brazilian medicinal plant widely used for the treatment of peptic ulcer and wound healing. Genotoxic, cytotoxic, antigenotoxic, and anticytotoxic effects of punicalagin were assessed using micronucleus (MN) test and comet assay in mice. Due to the extensive use of L. pacari in the wound healing process, we also assessed the angiogenic activity of punicalagin using the chick chorioallantoic membrane (CAM) angiogenic assay. The highest dose of punicalagin (50mg/kg) showed significant cytotoxic effect by MN test and in the co-treatment with cyclophosphamide (CPA), this cytotoxicity was enhanced. Co-treatment, pre-treatment and post-treatment of punicalagin with CPA led to a significant reduction in the number of DNA breaks and in the frequency of CPA-induced MN, indicating antigenotoxic effect. Using the CAM model, punicalagin exhibited angiogenic activity in all doses mainly at the lowest concentration (12.5μg/μL). Therefore, these findings indicate an effective chemopreventive role of punicalagin and a high capacity to induce DNA repair. Also, the angiogenic activity presented by punicalagin in this study could contribute for the processes of tissue repairing and wound healing.
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001970 Goiânia, GO, Brazil. Electronic address: profacristiene@gmail.com.
authors.forename: Cristiene Costa
authors.initials: CC
authors.lastname: Carneiro
authors.affiliation: Instituto de Química, Universidade Federal de Goiás, 74001970 Goiânia, GO, Brazil.
authors.forename: Suzana
authors.initials: S
authors.lastname: da Costa Santos
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, 74605050 Goiânia, GO, Brazil.
authors.forename: Ruy
authors.initials: R
authors.lastname: de Souza Lino
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Goiás, 74605170 Goiânia, GO, Brazil.
authors.forename: Maria Teresa Freitas
authors.initials: MT
authors.lastname: Bara
authors.affiliation: Faculdade de Farmácia, Universidade Federal de Goiás, 74605170 Goiânia, GO, Brazil.
authors.forename: Beatriz Abdallah
authors.initials: BA
authors.lastname: Chaibub
authors.affiliation: Laboratório de Estudos Experimentais e Biotecnológicos, Pontifícia Universidade Católica de Goiás, 74605010 Goiânia, GO, Brazil.
authors.forename: Paulo Roberto
authors.initials: PR
authors.lastname: de Melo Reis
authors.affiliation: Laboratório de Estudos Experimentais e Biotecnológicos, Pontifícia Universidade Católica de Goiás, 74605010 Goiânia, GO, Brazil.
authors.forename: Dwight Assis
authors.initials: DA
authors.lastname: Chaves
authors.affiliation: Núcleo de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, 21941590 Rio de Janeiro, RJ, Brazil.
authors.forename: Antônio Jorge Ribeiro
authors.initials: AJ
authors.lastname: da Silva
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001970 Goiânia, GO, Brazil.
authors.forename: Luana Santos
authors.initials: LS
authors.lastname: Silva
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001970 Goiânia, GO, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: de Melo E Silva
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001970 Goiânia, GO, Brazil.
authors.forename: Lee
authors.initials: L
authors.lastname: Chen-Chen
mesh_terms: D000818:Animals; D018890:Chemoprevention; D002642:Chick Embryo; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D029561:Lythraceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D047348:Hydrolyzable Tannins; C115642:punicalagin
keywords: Angiogenesis; Anticytotoxicity; Antigenotoxicity; Chemoprevention; DNA repair; Punicalagin