title: Dextran sulfate sodium-induced acute experimental colitis in C57BL/6 mice is mitigated by selenium.
abstract: BACKGROUND
  Sodium selenite has been shown to have a protective role in experimental colitis. Th1 and Th17 responses are involved in the pathogenesis of dextran sulfate sodium (DSS)-induced colitis and inflammatory bowel disease. This study investigated whether sodium selenite can suppress Th1/Th17-mediated experimental colitis.
  
  
  METHODS
  Mice were administered sodium selenite (2μg/g body weight) by gavage daily for 30days. Beginning on day 21, mice were administered 2.5% oral DSS for 9days. The mice were sacrificed on day 31. Survival rates, clinical symptoms, colon lengths, and histological changes were determined.
  
  
  RESULTS
  Pretreatment with sodium selenite (2μg/g body weight) improved survival rates, colon shortening, body weight loss, disease activity index, and histopathological score in mice with DSS-induced colitis. Pretreatment with sodium selenite restored interleukin-10 and Foxp3 excretion, as well as reducing the levels of interferon-γ and interleukin-17A.
  
  
  CONCLUSIONS
  Pretreatment with sodium selenite showed therapeutic potential for preventing colitis in mice. This effect may be mediated by the immunomodulation of regulatory T cells, expressing anti-inflammatory genes that suppress Th1 and Th17 responses.
authors.affiliation: Department of Immunology, China Medical University, Shenyang, China; Department of Geriatrics, First Affiliated Hospital, China Medical University, Shenyang, China. Electronic address: sanglixuan2008@163.com.
authors.forename: Lixuan
authors.initials: L
authors.lastname: Sang
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, China Medical University, Shenyang, China. Electronic address: cb000216@163.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Chang
authors.affiliation: Department of Immunology, China Medical University, Shenyang, China. Electronic address: zjf0709@126.com.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Immunology, China Medical University, Shenyang, China. Electronic address: yfl8412@163.com.
authors.forename: Fangli
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Immunology, China Medical University, Shenyang, China. Electronic address: licaorenyanyan@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Immunology, China Medical University, Shenyang, China. Electronic address: cmujxf@hotmail.com.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Immunology, China Medical University, Shenyang, China. Electronic address: wsunxun1220@hotmail.com.
authors.forename: Xun
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Immunology, China Medical University, Shenyang, China. Electronic address: cllu@mail.cmu.edu.cn.
authors.forename: Changlong
authors.initials: C
authors.lastname: Lu
authors.affiliation: Department of Immunology, China Medical University, Shenyang, China. Electronic address: wangdanan1972@126.com.
authors.forename: Danan
authors.initials: D
authors.lastname: Wang
mesh_terms: D000208:Acute Disease; D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D016264:Dextran Sulfate; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism*; D020381:Interleukin-17 / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D018038:Sodium Selenite / Q000627:therapeutic use*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D020381:Interleukin-17; D016753:Interleukin-10; D007371:Interferon-gamma; D016264:Dextran Sulfate; D018038:Sodium Selenite
keywords: DSS-induced colitis; Inflammatory bowel disease; Sodium selenite