title: Effective one-pot multienzyme (OPME) synthesis of monotreme milk oligosaccharides and other sialosides containing 4-O-acetyl sialic acid.
abstract: A facile one-pot two-enzyme chemoenzymatic approach has been established for the gram (Neu4,5Ac2α3Lac, 1.33 g) and preparative scale (Neu4,5Ac2α3LNnT) synthesis of monotreme milk oligosaccharides. Other O-acetyl-5-N-acetylneuraminic acid (Neu4,5Ac2)- or 4-O-acetyl-5-N-glycolylneuraminic acid (Neu4Ac5Gc) -containing α2-3-sialosides have also been synthesized in the preparative scale. Used as an effective probe, Neu4,5Ac2α3GalβpNP was found to be a suitable substrate by human influenza A viruses but not bacterial sialidases.
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA. xiichen@ucdavis.edu.
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Jie
authors.initials: J
authors.lastname: Zeng
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Li
authors.forename: Vireak
authors.initials: V
authors.lastname: Thon
authors.forename: Baojun
authors.initials: B
authors.lastname: Shi
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D008968:Molecular Conformation; D000216:N-Acylneuraminate Cytidylyltransferase / Q000378:metabolism*; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D012794:Sialic Acids / Q000096:biosynthesis* / Q000737:chemistry; D012799:Sialyltransferases / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D012794:Sialic Acids; D012799:Sialyltransferases; D000216:N-Acylneuraminate Cytidylyltransferase