title: Preparation of Cell Lysate from Mouse Oocytes for Western Blotting Analysis.
abstract: Western Blotting has been used extensively for the identification of the protein factors that regulate mammalian oocyte meiosis. However, the limitations in collecting sufficient numbers of oocytes can hinder the efficiency of the technique. Here we provide a detailed protocol for the accurate preparation of mouse oocyte samples for Western Blotting analysis.
authors.affiliation: Department of Biological Applications and Technology, University of Ioannina, Ioannina, 45110, Greece. pmaragos@cc.uoi.gr.|Department of Biomedical Research, Institute of Molecular Biology and Biotechnology-Foundation for Research and Technology(IMBB/FORTH), Ioannina, 45110, Greece. pmaragos@cc.uoi.gr.
authors.forename: Petros
authors.initials: P
authors.lastname: Marangos
mesh_terms: D000818:Animals; D015415:Biomarkers; D015153:Blotting, Western / Q000379:methods; D005260:Female; D051379:Mice; D009865:Oocytes / Q000378:metabolism*
chemical_list: D015415:Biomarkers
keywords: Chemiluminescence; HRP-conjugated antibody; Morpholino; Mouse oocyte; Primary antibody; SDS-PAGE; Securin; Western blotting