title: Regulation of the Coxsackie and adenovirus receptor expression is dependent on cystic fibrosis transmembrane regulator in airway epithelial cells.
abstract: The coxsackievirus and adenovirus receptor (CAR), in addition to serving as viral receptor, is a component of tight junctions and plays an important role in tissue homeostasis. Defects in the cystic fibrosis transmembrane regulator (CFTR) in lung epithelial cells are linked to inflammation and susceptibility for respiratory tract infections. Here, we demonstrate that CAR expression and infectivity with adenovirus (Ad) are increased in cystic fibrosis airway epithelial cells. Inhibition of CFTR or histone deacetylase (HDAC) enhanced CAR expression while CFTR overexpression or restoration of the diminished HDAC activity in cystic fibrosis cells reduced CAR expression. This connects the CFTR to CAR expression and infectivity with adenovirus through HDAC.
authors.affiliation: Department of Pediatrics, Weill Cornell Medicine, New York, New York, USA.
authors.forename: Anurag
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of Pediatrics, Weill Cornell Medicine, New York, New York, USA.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA.
authors.forename: Biin
authors.initials: B
authors.lastname: Sung
authors.affiliation: Department of Physiology and Biophysics, Weill Cornell Medicine, New York, New York, USA.|Department of Pharmacology and Physiology, Karolinska Institute, Stockholm, Sweden.
authors.forename: C Theresa
authors.initials: CT
authors.lastname: Vincent
authors.affiliation: Department of Pathology, Columbia University, New York, New York, USA.
authors.forename: Tilla
authors.initials: T
authors.lastname: Worgall
authors.affiliation: Department of Pediatrics, Weill Cornell Medicine, New York, New York, USA.|Department of Genetic Medicine, Weill Cornell Medicine, New York, New York, USA.
authors.forename: Stefan
authors.initials: S
authors.lastname: Worgall
mesh_terms: D000256:Adenoviridae / Q000254:growth & development; D002460:Cell Line; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein / Q000096:biosynthesis*; D003550:Cystic Fibrosis / Q000473:pathology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans
chemical_list: C505032:CFTR protein, human; C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D019005:Cystic Fibrosis Transmembrane Conductance Regulator