title: Gingival fibroblasts resist apoptosis in response to oxidative stress in a model of periodontal diseases.
abstract: Periodontal diseases are classified as inflammation affecting the supporting tissue of teeth, which eventually leads to tooth loss. Mild reversible gingivitis and severe irreversible periodontitis are the most common periodontal diseases. Periodontal pathogens initiate the diseases. The bacterial toxin, lipopolysaccharide (LPS), triggers the inflammatory response and leads to oxidative stress. However, the progress of oxidative stress in periodontal diseases is unknown. The purpose of this study is to examine oxidative stress and cell damage in gingivitis and periodontitis. Our results showed that LPS increases reactive oxygen species (ROS) accumulation in gingival fibroblast (GF). However, oxidative stress resulting from excessive ROS did not influence DNA damage and cell apoptosis within 24 h. The mechanism may be related to the increased expression of DNA repair genes, Ogg1, Neil1 and Rad50. Detection of apoptosis-related proteins also showed anti-apoptotic effects and pro-apoptotic effects were balanced. The earliest damage appeared in DNA when increased γH2AX, an early biomarker for DNA damage, was detected in the LPS group after 48 h. Later, when recurrent inflammation persisted, 8-OHdG, a biomarker for oxidative stress was much higher in periodontitis model compared to the control in vivo. Staining of 8-OHdG in human periodontitis specimens confirmed the results. Furthermore, TUNEL staining of apoptotic cells indicated that the periodontitis model induced more cell apoptosis in gingival tissue. This suggested GF could resist early and acute inflammation (gingivitis), which was regarded as reversible, but recurrent and chronic inflammation (periodontitis) led to permanent cell damage and death.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China; Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: R
authors.initials: R
authors.lastname: Cheng
authors.affiliation: Cedars-Sinai Medical Center , Los Angeles, CA, USA.
authors.forename: D
authors.initials: D
authors.lastname: Choudhury
authors.affiliation: Cedars-Sinai Medical Center, Los Angeles, CA, USA; Affiliated Hospital of Stomatology, Zhejiang University, Hangzhou, China.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.affiliation: Cedars-Sinai Medical Center , Los Angeles, CA, USA.
authors.forename: S
authors.initials: S
authors.lastname: Billet
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University , Chengdu, China.
authors.forename: T
authors.initials: T
authors.lastname: Hu
authors.affiliation: Cedars-Sinai Medical Center , Los Angeles, CA, USA.
authors.forename: N A
authors.initials: NA
authors.lastname: Bhowmick