title: [Effect of Interferon Combined with Thalidomid on HEL Cell Apoptosis and JAK2V617F Mutation Gene Expression].
abstract: OBJECTIVE
  To investigate the effects of interferon-α2b combined with thalidomid(THD) on the proliferation and apoptosis of HEL cells, and expression of JAK2V617F-mutated gene.
  
  
  METHODS
  The cell survival rates were assayed by CCK-8 after HEL cells were treated by interferon-α2b, thalidomid and thalidomid combined with interferon-α2b for 24, 48 and 72 hours, while the apoptosis and expression of JAK2V617F mutation gene were detected by flow cytometry and fluorescence quantitative PCR respectively after treatmemt for 48 hours.
  
  
  RESULTS
  IFN-α2b combined with THD could more obviously inhibit the proliferation of HEL cells than IFN-α2b or THD alone for 24 and 48 and 72 hours, and the differences between groups were statistically significant(P<0.05). In addition, the apoptosis-inducing effect of IFN-α2b combined with THD on HEL cells was more obvious than that of IFN-α2b used alone, the differences was statistically significant(P<0.05). Although IFN-α2b combined with THD can decrease the JAK2V617F mutation gene expression more obviously than IFN-α2b alone,but the difference was no statistically significant (P>0.05). And the remaining groups showed statistically significance(P<0.05).
  
  
  CONCLUSIONS
  IFN-α2b combined with THD can obviously inhibit the proliferation and induce apoptosis of HEL cells more than that of IFN-α2b alone, but the effect of reducing JAK2V617F mutation gene expression is not different.
authors.affiliation: Department of Hematology, Lianyungang Hospital Affiliated to Xuzhou Medical School, Lianyungang 222000, Jiangsu Province, China.
authors.forename: Xiao-Xie
authors.initials: XX
authors.lastname: Wu
authors.affiliation: Department of Hematology, Lianyungang Hospital Affiliated to Xuzhou Medical School, Lianyungang 222000, Jiangsu Province, China.
authors.forename: Lian-Guo
authors.initials: LG
authors.lastname: Xue
authors.affiliation: Department of Hematology, Lianyungang Hospital Affiliated to Xuzhou Medical School, Lianyungang 222000, Jiangsu Province, China. E-mail: zld060201@sina.com.
authors.forename: Li-Dong
authors.initials: LD
authors.lastname: Zhao
authors.affiliation: Department of Hematology, Lianyungang Hospital Affiliated to Xuzhou Medical School, Lianyungang 222000, Jiangsu Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Hematology, Lianyungang Hospital Affiliated to Xuzhou Medical School, Lianyungang 222000, Jiangsu Province, China.
authors.forename: Zhi-Mei
authors.initials: ZM
authors.lastname: Cai
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016898:Interferon-alpha; D053614:Janus Kinase 2; D009154:Mutation; D013792:Thalidomide
chemical_list: D016898:Interferon-alpha; D013792:Thalidomide; C507924:JAK2 protein, human; D053614:Janus Kinase 2