title: Fluorescence Talbot microscope using incoherent source.
abstract: Fluorescence Talbot microscope is a scalable field-of-view (FOV) imaging platform, which takes advantage of the phase sensitivity of the self-image of a periodic structure. Such a system can maintain the microscopic resolution and extend the FOV for the whole slide (15  mm×15  mm) scanning. Previously reported Talbot fluorescence systems, tabletop and on-chip device alike, rely on the coherence of the illumination source, limiting their potential applications in low-resource setting environment. A more cost-effective setup using a light-emitting diode, which has an area of 4  mm2 and a full width at half maximum of 16 nm in wavelength, is demonstrated. Compared to the illumination that is spatially filtered by a single pinhole, our system has achieved an illumination intensity that is 357 times higher. The reconstructed image quality is comparable to that of a 10× microscope objective. Various samples, such as fluorescent beads, green fluorescence protein-labeled HeLa cells, and a mouse kidney slide, were reconstructed by the system.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Sun
authors.forename: Shuo
authors.initials: S
authors.lastname: Pang
mesh_terms: D000818:Animals; D003362:Cost-Benefit Analysis; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008029:Lighting; D051379:Mice; D008856:Microscopy, Fluorescence / Q000191:economics / Q000295:instrumentation*; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins