title: Identification of functional farnesoid X receptors in brain neurons.
abstract: Farnesoid X receptor (FXR) has been confirmed to sense bile acids in various tissues. However, its expression in brain neurons remains obscure. In this study, we identified FXR mRNA and protein expression in mouse brain neurons and in mouse/human brain tissues. FXR was predominantly localized in the nucleus in cultured neurons, but in neurons in vivo, it mainly appeared in the cytoplasm. In nuclear compartments, the neuronal FXR exhibited a punctate distribution. Activation of FXR increased the small heterodimer partner (SHP) mRNA and protein expression levels in cultured neurons and in brain tissues. These findings will help explore new functions of FXR in the brain.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, China.|Key Laboratory of Inflammation and Molecular Drug Target of Jiangsu, Nantong, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, China.|Key Laboratory of Inflammation and Molecular Drug Target of Jiangsu, Nantong, China.
authors.forename: Jili
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, China.|Key Laboratory of Inflammation and Molecular Drug Target of Jiangsu, Nantong, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Hu
authors.affiliation: Basic Medical Research Center, Medical College, Nantong University, China.
authors.forename: Chengniu
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, China.|Key Laboratory of Inflammation and Molecular Drug Target of Jiangsu, Nantong, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, China.|Key Laboratory of Inflammation and Molecular Drug Target of Jiangsu, Nantong, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Tong
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, China.|Key Laboratory of Inflammation and Molecular Drug Target of Jiangsu, Nantong, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, School of Pharmacy, Nantong University, China. asicampa@aliyun.com.|Key Laboratory of Inflammation and Molecular Drug Target of Jiangsu, Nantong, China. asicampa@aliyun.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D000099150:Receptor, Farnesoid X-Activated
chemical_list: D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D000099150:Receptor, Farnesoid X-Activated; C099713:nuclear receptor subfamily 0, group B, member 2
keywords: brain; farnesoid X receptor; neuron; small heterodimer partner