title: A novel flow injection spectrophotometric method using plant extracts as green reagent for the determination of doxycycline.
abstract: A novel flow injection spectrophotometric method was developed for the determination of doxycycline in pharmaceutical preparations using iron(III) contained in extracts from plants. The assay was based on the complex formed between doxycycline and iron(III) characterized by an absorption maximum at 435nm. The calibration graphs obtained over the doxycycline concentration range 5-250μgmL-1gave correlation coefficients of 0.9979, 0.9987 and 0.9987 with the three green reagents prepared from Senna alata (L.) Roxb. (S. alata), Polygonum hydropiper L. (P. hydropiper) or Diplazium esculentum (Retz.) Sw. (D. esculentum), respectively. The relative standard deviations of the repeatability was <2.00%. The percentage recoveries were in the range of 98.27-101.03%. Doxycycline contents obtained by this new method and by the reference methods reported in literature were in agreement at 95% confidence level with the paired t-test. The sample throughput was 36h-1for each green reagent.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Sysay
authors.initials: S
authors.lastname: Palamy
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: wirat_ru@kku.ac.th.
authors.forename: Wirat
authors.initials: W
authors.lastname: Ruengsitagoon
mesh_terms: D004318:Doxycycline / Q000032:analysis* / Q000737:chemistry; D017022:Flow Injection Analysis / Q000379:methods*; D006851:Hydrochloric Acid / Q000737:chemistry; D007202:Indicators and Reagents; D007501:Iron / Q000737:chemistry; D057230:Limit of Detection; D017942:Nitric Acid / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D007202:Indicators and Reagents; D010936:Plant Extracts; D012997:Solvents; D017942:Nitric Acid; D007501:Iron; D004318:Doxycycline; D006851:Hydrochloric Acid
keywords: Doxycycline; Flow injection analysis; Green reagent; Iron(III)