title: Nitrate Controls Root Development through Posttranscriptional Regulation of the NRT1.1/NPF6.3 Transporter/Sensor.
abstract: Plants are able to modulate root growth and development to optimize their nitrogen nutrition. In Arabidopsis (Arabidopsis thaliana), the adaptive root response to nitrate (NO3-) depends on the NRT1.1/NPF6.3 transporter/sensor. NRT1.1 represses emergence of lateral root primordia (LRPs) at low concentration or absence of NO3-through its auxin transport activity that lowers auxin accumulation in LR. However, these functional data strongly contrast with the known transcriptional regulation of NRT1.1, which is markedly repressed in LRPs in the absence of NO3-To explain this discrepancy, we investigated in detail the spatiotemporal expression pattern of the NRT1.1 protein during LRP development and combined local transcript analysis with the use of transgenic lines expressing tagged NRT1.1 proteins. Our results show that although NO3-stimulates NRT1.1 transcription and probably mRNA stability both in primary root tissues and in LRPs, it acts differentially on protein accumulation, depending on the tissues considered with stimulation in cortex and epidermis of the primary root and a strong repression in LRPs and to a lower extent at the primary root tip. This demonstrates that NRT1.1 is strongly regulated at the posttranscriptional level by tissue-specific mechanisms. These mechanisms are crucial for controlling the large palette of adaptive responses to NO3-mediated by NRT1.1 as they ensure that the protein is present in the proper tissue under the specific conditions where it plays a signaling role in this particular tissue.
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/SupAgro-M/UM2, Montpellier 34060, France (E.Bo., F.P.-W., M.P., J.R., A.M., L.B., G.K., A.G., P.N.); andInstitute of Science and Technology Austria, A-3400 Klosterneuburg, Austria (C.C., E.Ba.).
authors.forename: Eléonore
authors.identifier: 0000-0002-6501-5823
authors.initials: E
authors.lastname: Bouguyon
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/SupAgro-M/UM2, Montpellier 34060, France (E.Bo., F.P.-W., M.P., J.R., A.M., L.B., G.K., A.G., P.N.); andInstitute of Science and Technology Austria, A-3400 Klosterneuburg, Austria (C.C., E.Ba.).
authors.forename: Francine
authors.initials: F
authors.lastname: Perrine-Walker
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/SupAgro-M/UM2, Montpellier 34060, France (E.Bo., F.P.-W., M.P., J.R., A.M., L.B., G.K., A.G., P.N.); andInstitute of Science and Technology Austria, A-3400 Klosterneuburg, Austria (C.C., E.Ba.).
authors.forename: Marjorie
authors.identifier: 0000-0001-8496-2474
authors.initials: M
authors.lastname: Pervent
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/SupAgro-M/UM2, Montpellier 34060, France (E.Bo., F.P.-W., M.P., J.R., A.M., L.B., G.K., A.G., P.N.); andInstitute of Science and Technology Austria, A-3400 Klosterneuburg, Austria (C.C., E.Ba.).
authors.forename: Juliette
authors.initials: J
authors.lastname: Rochette
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/SupAgro-M/UM2, Montpellier 34060, France (E.Bo., F.P.-W., M.P., J.R., A.M., L.B., G.K., A.G., P.N.); andInstitute of Science and Technology Austria, A-3400 Klosterneuburg, Austria (C.C., E.Ba.).
authors.forename: Candela
authors.identifier: 0000-0003-1923-2410
authors.initials: C
authors.lastname: Cuesta
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/SupAgro-M/UM2, Montpellier 34060, France (E.Bo., F.P.-W., M.P., J.R., A.M., L.B., G.K., A.G., P.N.); andInstitute of Science and Technology Austria, A-3400 Klosterneuburg, Austria (C.C., E.Ba.).
authors.forename: Eva
authors.identifier: 0000-0002-8510-9739
authors.initials: E
authors.lastname: Benkova
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/SupAgro-M/UM2, Montpellier 34060, France (E.Bo., F.P.-W., M.P., J.R., A.M., L.B., G.K., A.G., P.N.); andInstitute of Science and Technology Austria, A-3400 Klosterneuburg, Austria (C.C., E.Ba.).
authors.forename: Alexandre
authors.initials: A
authors.lastname: Martinière
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/SupAgro-M/UM2, Montpellier 34060, France (E.Bo., F.P.-W., M.P., J.R., A.M., L.B., G.K., A.G., P.N.); andInstitute of Science and Technology Austria, A-3400 Klosterneuburg, Austria (C.C., E.Ba.).
authors.forename: Lien
authors.initials: L
authors.lastname: Bach
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/SupAgro-M/UM2, Montpellier 34060, France (E.Bo., F.P.-W., M.P., J.R., A.M., L.B., G.K., A.G., P.N.); andInstitute of Science and Technology Austria, A-3400 Klosterneuburg, Austria (C.C., E.Ba.).
authors.forename: Gabriel
authors.identifier: 0000-0003-3693-6735
authors.initials: G
authors.lastname: Krouk
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/SupAgro-M/UM2, Montpellier 34060, France (E.Bo., F.P.-W., M.P., J.R., A.M., L.B., G.K., A.G., P.N.); andInstitute of Science and Technology Austria, A-3400 Klosterneuburg, Austria (C.C., E.Ba.).
authors.forename: Alain
authors.initials: A
authors.lastname: Gojon
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique/SupAgro-M/UM2, Montpellier 34060, France (E.Bo., F.P.-W., M.P., J.R., A.M., L.B., G.K., A.G., P.N.); andInstitute of Science and Technology Austria, A-3400 Klosterneuburg, Austria (C.C., E.Ba.).
authors.forename: Philippe
authors.identifier: 0000-0001-7766-4989
authors.initials: P
authors.lastname: Nacry
mesh_terms: D027321:Anion Transport Proteins / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018519:Meristem / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D009154:Mutation; D009566:Nitrates / Q000378:metabolism*; D009928:Organ Specificity / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D020871:RNA Stability / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097573:Red Fluorescent Protein
chemical_list: D027321:Anion Transport Proteins; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D009566:Nitrates; D010940:Plant Proteins; D000097573:Red Fluorescent Protein; C101492:NRT1.1 protein, Arabidopsis