title: DSBCapture: in situ capture and sequencing of DNA breaks.
abstract: Double-strand DNA breaks (DSBs) continuously arise and cause mutations and chromosomal rearrangements. Here, we present DSBCapture, a sequencing-based method that captures DSBs in situ and directly maps these at single-nucleotide resolution, enabling the study of DSB origin. DSBCapture shows substantially increased sensitivity and data yield compared with other methods. Using DSBCapture, we uncovered a striking relationship between DSBs and elevated transcription within nucleosome-depleted chromatin.
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
authors.forename: Stefanie V
authors.initials: SV
authors.lastname: Lensing
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Marsico
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
authors.forename: Robert
authors.initials: R
authors.lastname: Hänsel-Hertsch
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
authors.forename: Enid Y
authors.initials: EY
authors.lastname: Lam
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
authors.forename: David
authors.initials: D
authors.lastname: Tannahill
authors.affiliation: Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.|Department of Chemistry, University of Cambridge, Cambridge, UK.|School of Clinical Medicine, University of Cambridge, Cambridge, UK.
authors.forename: Shankar
authors.initials: S
authors.lastname: Balasubramanian
mesh_terms: D018929:Cell Culture Techniques; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000235:genetics*; D053903:DNA Breaks, Double-Stranded; D059766:DNA End-Joining Repair / Q000235:genetics; D044127:Epigenesis, Genetic; D006367:HeLa Cells; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D002843:Chromatin; D004247:DNA