title: Effect of in vivo and in vitro exposure to electrostatic field on some hematological parameters in rats.
abstract: The aim of this study was to investigate the effect of the external electrostatic field (ESF) on some hematological parameters in rats. Both in vivo and in vitro experiments were carried out. In in vivo investigations, rats were exposed to ESF (200 kV/m) during short (1 h) and long periods (6 days, 6 h daily). For in vitro study, the blood of intact rats was exposed to ESF for 1 h. Blood hematology was measured using validated ABX Micros ESV 60 Veterinary Hematology Analyzer. DNA damage in blood leucocytes was detected by means of comet assay. ESF effect on blood cell count was mainly manifested in white blood cells (WBC) and platelets. Damage of WBC was shown both in vitro and in vivo despite alterations in the count. This means the observed increase in WBC count in some cases might be a result of WBC compensatory mobilization from the bone marrow. Red blood cell (RBC) count and related parameters were slightly affected by ESF. Nevertheless, alterations in the shape and size of RBC were manifested. All ESF effects were extinguished in 14 days after the end of exposure. Bioelectromagnetics. 37:513-526, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Laboratory of Biochemical and Biophysical Investigations, Scientific-Research Centre, Yerevan State Medical University After Mkhitar Heratsi, Yerevan, Republic of Armenia.|Laboratory of Adenyline Compaunds Metabolism, H. Buniatian Institute of Biochemistry, National Academy of Sciences of Republic of Armenia, Yerevan, Republic of Armenia.
authors.forename: Hayk
authors.initials: H
authors.lastname: Harutyunyan
authors.affiliation: Chair of Therapy Clinical Diagnostics and Pharmacology, Faculty of Veterinary Medicine and Animal Husbandry, Armenian National Agrarian University, Yerevan, Republic of Armenia.
authors.forename: Vahe
authors.initials: V
authors.lastname: Mkrtchyan
authors.affiliation: Chair of Therapy Clinical Diagnostics and Pharmacology, Faculty of Veterinary Medicine and Animal Husbandry, Armenian National Agrarian University, Yerevan, Republic of Armenia.
authors.forename: Karine
authors.initials: K
authors.lastname: Sukiasyan
authors.affiliation: Laboratory of Biochemical and Biophysical Investigations, Scientific-Research Centre, Yerevan State Medical University After Mkhitar Heratsi, Yerevan, Republic of Armenia.
authors.forename: Gohar
authors.initials: G
authors.lastname: Sahakyan
authors.affiliation: Laboratory of Biochemical and Biophysical Investigations, Scientific-Research Centre, Yerevan State Medical University After Mkhitar Heratsi, Yerevan, Republic of Armenia.
authors.forename: Gayane
authors.initials: G
authors.lastname: Poghosyan
authors.affiliation: Laboratory of Biochemical and Biophysical Investigations, Scientific-Research Centre, Yerevan State Medical University After Mkhitar Heratsi, Yerevan, Republic of Armenia.
authors.forename: Ani
authors.initials: A
authors.lastname: Soghomonyan
authors.affiliation: Laboratory of Biochemistry of Drugs, Research Institute for Physical Chemical Problems, Belarusian State University, Minsk, Republic of Belarus.
authors.forename: Eugene
authors.initials: E
authors.lastname: Cherniavsky
authors.affiliation: Laboratory of Biochemistry of Drugs, Research Institute for Physical Chemical Problems, Belarusian State University, Minsk, Republic of Belarus.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Bondarenko
authors.affiliation: Laboratory of Biochemistry of Drugs, Research Institute for Physical Chemical Problems, Belarusian State University, Minsk, Republic of Belarus.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Shkumatov
keywords: DNA-comet test; electrostatic field; platelets; red blood cells; white blood cells