title: Oral vitamin D supplementation at five times the recommended allowance marginally affects serum 25-hydroxyvitamin D concentrations in dogs.
abstract: Little is known regarding optimal vitamin D status in adult dogs. To date no studies on vitamin D supplementation for improving vitamin D status have been reported for adult dogs. The aims of this study were to identify dogs with low vitamin D status and evaluate an oral dosage of cholecalciferol (D3) for effectiveness in increasing vitamin D status. For this, forty-six privately owned dogs were evaluated. Of the dogs, thirty-three (or 71·7 %) had serum 25-hydroxyvitamin D (25(OH)D) concentrations less than 100 ng/ml, a minimum previously suggested for vitamin D sufficiency in dogs. Subsequently, thirteen dogs were enrolled in a supplementation trial. Dogs were given either a D3 supplement (n 7; 2·3 µg/kg(0·75)) or olive oil placebo (n 6) daily with food. Serum concentrations of 25(OH)D were determined at weeks 1, 3 and 6, and at the trial end. Only at the trial end (weeks 9-10) was 25(OH)D significantly greater (P = 0·05) in supplemented v. placebo dogs. Serum concentrations of 24R,25-dihydroxycholecalciferol determined at the trial end were about 40 % of that of 25(OH)D3 and not significantly different between the groups. Concentrations of parathyroid hormone, ionised Ca, P and creatinine measured in initial and final serum samples indicated supplementation caused no toxicity. We conclude that vitamin D3 supplementation at a dosage near the National Research Council recommended safe-upper limit was not effective for rapidly raising serum 25(OH)D concentrations in healthy, adult dogs. Further work is needed in evaluating the metabolism of orally administered D3 in dogs before dosing recommendations can be made.
authors.affiliation: Department of Veterinary Medicine and Surgery , College of Veterinary Medicine , University of Missouri , Columbia , MO 65211 , USA.
authors.forename: Lauren R
authors.initials: LR
authors.lastname: Young
authors.affiliation: Department of Veterinary Medicine and Surgery , College of Veterinary Medicine , University of Missouri , Columbia , MO 65211 , USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Backus
keywords: 1,25(OH)2D3, 1,25-dihydroxyvitamin D3; 24R, 25-dihydroxycholecalciferol; 24R,25(OH)2D3, 24R,25-dihydroxyvitamin D3; 25(OH)D, 25-hydroxyvitamin D; 25(OH)D2, 25-hydroxyergocalciferol; 25(OH)D3, 25-hydroxycholecalciferol; 25-Hydroxyergocalciferol; BW, body weight; CLIA, chemiluminesence immunoassay; Cholecalciferol; Ionised calcium; NRC, National Research Council; PTH, parathyroid hormone; Parathyroid hormone; iCa, ionised Ca