title: Correlation between ZBED6 Gene Upstream CpG Island methylation and mRNA expression in cattle.
abstract: DNA methylation is essential for the regulation of gene expression and important roles in muscle development. To assess the extent of epigenetic modifications and gene expression on the differentially methylated region (DMR) in ZBED6, we simultaneously examined DNA methylation and expression in six tissues from two different developmental stages (fetal bovine and adult bovine). The DNA methylation pattern was compared using bisulfite sequencing polymerase chain reaction (BSP) and combined bisulfite restriction analysis (COBRA). The result of quantitative real-time PCR (qPCR) analysis showed that ZBED6 has a broad tissue distribution and is highly expressed in adult bovine (P < 0.05 or P < 0.01). The DNA methylation level was significantly different in liver, lung and spleen between the two cattle groups (P < 0.05 or P < 0.01). The adult bovine group exhibited a significantly higher mRNA level and lower DNA methylation level than the fetal bovine group in liver, lung, and spleen. No significant association was detected between DNA methylation level and muscle, heart, and kidney at two different stages. In this study, the statistical analyses indicated that DNA methylation patterns are associated with mRNA level in some tissues, these results may be a useful parameter to investigate muscle developmental in cattle and as a model for studies in other species, potentially contributing to an improvement of growth performance selection in beef cattle breeding program.
authors.affiliation: a College of Animal Science and Technology , Northwest A&F University , Yangling Shaanxi , People's Republic of China.
authors.forename: Yong-Zhen
authors.initials: YZ
authors.lastname: Huang
authors.affiliation: b Institute of Animal Husbandry and Veterinary Science , Henan Academy of Agricultural Sciences , Zhengzhou Henan , People's Republic of China.
authors.forename: Zi-Jing
authors.initials: ZJ
authors.lastname: Zhang
authors.affiliation: a College of Animal Science and Technology , Northwest A&F University , Yangling Shaanxi , People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.affiliation: a College of Animal Science and Technology , Northwest A&F University , Yangling Shaanxi , People's Republic of China.
authors.forename: Xiu-Kai
authors.initials: XK
authors.lastname: Cao
authors.affiliation: a College of Animal Science and Technology , Northwest A&F University , Yangling Shaanxi , People's Republic of China.
authors.forename: Cheng-Chuang
authors.initials: CC
authors.lastname: Song
authors.affiliation: a College of Animal Science and Technology , Northwest A&F University , Yangling Shaanxi , People's Republic of China.
authors.forename: Kun-Peng
authors.initials: KP
authors.lastname: Liu
authors.affiliation: a College of Animal Science and Technology , Northwest A&F University , Yangling Shaanxi , People's Republic of China.
authors.forename: Xian-Yong
authors.initials: XY
authors.lastname: Lan
authors.affiliation: a College of Animal Science and Technology , Northwest A&F University , Yangling Shaanxi , People's Republic of China.
authors.forename: Chu-Zhao
authors.initials: CZ
authors.lastname: Lei
authors.affiliation: d Bureau of Animal Husbandry of Biyang County , Biyang , Henan , People's Republic of China.
authors.forename: Xing-Lei
authors.initials: XL
authors.lastname: Qi
authors.affiliation: c Animal Health Supervision in Henan Province , Zhengzhou , Henan , People's Republic of China.
authors.forename: Yue-Yu
authors.initials: YY
authors.lastname: Bai
authors.affiliation: a College of Animal Science and Technology , Northwest A&F University , Yangling Shaanxi , People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002417:Cattle / Q000196:embryology* / Q000502:physiology*; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D009928:Organ Specificity / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D013223:Statistics as Topic; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D012097:Repressor Proteins
keywords: BSP; DNA methylation; ZBED6; cattle; qPCR