title: Detection and Quantification of the Entomopathogenic Fungal Endophyte Beauveria bassiana in Plants by Nested and Quantitative PCR.
abstract: The described protocol allows detecting as low as 10 fg the entomopathogenic fungal endophyte Beauveria bassiana in host plants by using a two-step nested PCR with the ITS1F/ITS4 and BB.fw and BB.rv primer pairs. On the other hand, a qPCR protocol using BB.fw and BB.rv primers is also available allowing the quantification of up to 26 fg of B. bassiana DNA per 20 ng of leaf DNA.
authors: Department of Agricultural and Forest Science and Resources, University of Córdoba (UCO), Campus de Rabanales, CeiA3, P.O. Box 3048, 14080, Córdoba, Spain. g72gajui@uco.es.
authors: Inmaculada
authors: I
authors: Garrido-Jurado
authors: Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Campus de Excelencia en Agroalimentación, CeiA3, P.O. Box 4084, 14080, Córdoba, Spain.
authors: Blanca B
authors: BB
authors: Landa
authors: Department of Agricultural and Forest Science and Resources, University of Córdoba (UCO), Campus de Rabanales, CeiA3, P.O. Box 3048, 14080, Córdoba, Spain.
authors: Enrique
authors: E
authors: Quesada-Moraga
mesh_terms: D052982:Beauveria / Q000235:genetics*; D015169:Colony Count, Microbial; D004271:DNA, Fungal; D021901:DNA, Intergenic; D060026:Endophytes / Q000235:genetics*; D016533:Mycological Typing Techniques; D010944:Plants / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic
keywords: DNA; Leaves; Mitosporic ascomycetes; Nested PCR; Quantitative PCR