title: Feruloyl esterases from Schizophyllum commune to treat food industry side-streams.
abstract: Agro-industrial side-streams are abundant and renewable resources of hydroxycinnamic acids with potential applications as antioxidants and preservatives in the food, health, cosmetic, and pharmaceutical industries. Feruloyl esterases (FAEs) from Schizophyllum commune were functionally expressed in Pichia pastoris with extracellular activities of 6000UL(-1). The recombinant enzymes, ScFaeD1 and ScFaeD2, released ferulic acid from destarched wheat bran and sugar beet pectin. Overnight incubation of coffee pulp released caffeic (>60%), ferulic (>80%) and p-coumaric acid (100%) indicating applicability for the valorization of food processing wastes and enhanced biomass degradation. Based on substrate specificity profiling and the release of diferulates from destarched wheat bran, the recombinant FAEs were characterized as type D FAEs. ScFaeD1 and ScFaeD2 preferably hydrolyzed feruloylated saccharides with ferulic acid esterified to the O-5 position of arabinose residues and showed an unprecedented ability to hydrolyze benzoic acid esters.
authors: Institut für Lebensmittelchemie, Leibniz Universität Hannover, Callinstraße 5, 30167 Hannover, Germany. Electronic address: annabel.nieter@lci.uni-hannover.de.
authors: Annabel
authors: A
authors: Nieter
authors: Institut für Lebensmittelchemie, Leibniz Universität Hannover, Callinstraße 5, 30167 Hannover, Germany.
authors: Sebastian
authors: S
authors: Kelle
authors: Institut für Lebensmittelchemie, Leibniz Universität Hannover, Callinstraße 5, 30167 Hannover, Germany.
authors: Diana
authors: D
authors: Linke
authors: Institut für Lebensmittelchemie, Leibniz Universität Hannover, Callinstraße 5, 30167 Hannover, Germany.
authors: Ralf G
authors: RG
authors: Berger
mesh_terms: D019817:Benzoic Acid / Q000737:chemistry / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D003069:Coffee / Q000737:chemistry; D003373:Coumaric Acids / Q000378:metabolism*; D004043:Dietary Fiber / Q000378:metabolism; D019649:Food Industry; D006868:Hydrolysis; D007220:Industrial Waste; D010368:Pectins / Q000737:chemistry / Q000378:metabolism; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011422:Propionates; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012567:Schizophyllum / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D003069:Coffee; D003373:Coumaric Acids; D004043:Dietary Fiber; D007220:Industrial Waste; D011422:Propionates; D011994:Recombinant Proteins; D010368:Pectins; D019817:Benzoic Acid; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; C495469:p-coumaric acid
keywords: Agro-industrial side-streams; Basidiomycota; Benzoic acid ester; Feruloyl esterase; Pichia pastoris