title: The role of a novel C-type lectin-like protein from planarian in innate immunity and regeneration.
abstract: Planarian, a representative of platyhelminthes, has strong regeneration ability and less complicated innate immune system. However, planarian immune system remains poorly understood. In this paper, a novel C-type lectin-like protein, namely, DjCTL was identified and characterized in Dugesia japonica. DjCTL was mainly expressed in the pharyngeal and epidermis and up-regulated upon the induction of lipopolysaccharide (LPS), peptidoglycan (PGN), Gram-positive and Gram-negative bacteria indicating that DjCTL may be involved in the immune responses. Recombination DjCTL protein agglomerated rabbit red blood cells and interacted with LPS, PGN, mannose and galactose as well as both Gram-positive and Gram-negative bacteria, but it can only cause the agglutination of Gram-negative bacteria. Importantly, in the early periods of regeneration, DjCTL had a significantly high expression and was mainly expressed in early blastemas. RNA interference of DjCTL by dsRNA-DjCTL led to a slow wound healing during regeneration. These findings suggest that DjCTL participates in the innate immune response and plays an important role in early stages of regeneration.
authors: Laboratory of Developmental and Evolutionary Biology, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China; Anti-Aging & Regenerative Medicine Research Institution, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China.
authors: Lili
authors: L
authors: Gao
authors: Laboratory of Developmental and Evolutionary Biology, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China; Anti-Aging & Regenerative Medicine Research Institution, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China.
authors: Yu
authors: Y
authors: Han
authors: Laboratory of Developmental and Evolutionary Biology, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China.
authors: Hongkuan
authors: H
authors: Deng
authors: Laboratory of Developmental and Evolutionary Biology, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China; Anti-Aging & Regenerative Medicine Research Institution, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China.
authors: Wenjing
authors: W
authors: Hu
authors: Laboratory of Developmental and Evolutionary Biology, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China.
authors: Hui
authors: H
authors: Zhen
authors: Laboratory of Developmental and Evolutionary Biology, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China; Anti-Aging & Regenerative Medicine Research Institution, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China.
authors: Na
authors: N
authors: Li
authors: Laboratory of Developmental and Evolutionary Biology, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China.
authors: Nianci
authors: N
authors: Qin
authors: Laboratory of Developmental and Evolutionary Biology, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China.
authors: Meihui
authors: M
authors: Yan
authors: Anti-Aging & Regenerative Medicine Research Institution, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China.
authors: Weiwei
authors: W
authors: Wu
authors: Anti-Aging & Regenerative Medicine Research Institution, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China; Shenzhen University of Health Science Center, District Shenzhen, 518060, PR China. Electronic address: ppliew@szu.edu.cn.
authors: Baohua
authors: B
authors: Liu
authors: Laboratory of Developmental and Evolutionary Biology, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China. Electronic address: zhaobosheng@sdut.edu.cn.
authors: Bosheng
authors: B
authors: Zhao
authors: Laboratory of Developmental and Evolutionary Biology, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China; Anti-Aging & Regenerative Medicine Research Institution, School of Life Sciences, Shandong University of Technology, Zibo, 255049, PR China. Electronic address: pangqiuxiang@163.com.
authors: Qiuxiang
authors: Q
authors: Pang
mesh_terms: D000818:Animals; D001419:Bacteria / Q000276:immunology / Q000378:metabolism*; D001424:Bacterial Infections / Q000276:immunology*; D003001:Cloning, Molecular; D006384:Hemagglutination; D007113:Immunity, Innate; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D010457:Peptidoglycan / Q000378:metabolism; D010802:Phylogeny; D010932:Planarians / Q000276:immunology*; D011485:Protein Binding; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000378:metabolism*; D012038:Regeneration; D015854:Up-Regulation
chemical_list: D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D051192:Receptors, Pattern Recognition
keywords: C-type lectin; Functional analysis; Innate immunity; Planarian; Regeneration