title: Enhanced Migration of Neural Stem Cells by Microglia Grown on a Three-Dimensional Graphene Scaffold.
abstract: One of the key challenges in engineering neural tissues for cell-based therapies is to develop a biocompatible scaffold material to direct neural stem cell (NSC) behaviors. One great advantage for a scaffold would be to induce NSC migration toward pathological sites during regeneration and repair. In particular, the inflammatory responses in the pathological zone, which are mainly mediated by microglia in the central nervous system, affect the repair capacity of NSCs through NSC migration. Recently, graphene was used as a neural interface and scaffold material, but few studies have addressed the relationship between microglia and NSCs in a graphene culture system. In this study, we used a combination of immunofluorescence, Western blotting, enzyme-linked immunosorbent assays, and scanning electron microscopy to investigate how conditioned medium (CM) produced from microglia grown on two-dimensional graphene (2D-G) films or three-dimensional graphene (3D-G) foams govern NSC migration. The results revealed that the CM produced by microglia grown in 3D-G cultures could promote neurosphere formation, facilitate NSC migration from the neurospheres, and increase single cell polarization by activating the stromal cell-derived factor 1 α (SDF-1α)/CXC chemokine receptor 4 (CXCR4) signaling pathway and enhancing cell adhesion on the substrate. By contrast, the 2D-G CM failed to achieve these results. Our study suggests the great potential of 3D-G as a neural scaffold for NSC-based therapy in tissue engineering and regenerative medicine.
authors: Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Jiangsu 215123, PR China.
authors: Ziyun
authors: Z
authors: Jiang
authors: Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Jiangsu 215123, PR China.
authors: Qin
authors: Q
authors: Song
authors: Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Jiangsu 215123, PR China.|Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University , Jiangsu 210018, PR China.
authors: Mingliang
authors: M
authors: Tang
authors: Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Jiangsu 215123, PR China.
authors: Lingyan
authors: L
authors: Yang
authors: Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Jiangsu 215123, PR China.
authors: Yilin
authors: Y
authors: Cheng
authors: Center for Neuroimmunology and Regenerative Therapy, Shanghai Tenth People's Hospital, School of Medicine, Tongji University , Shanghai 200092, PR China.
authors: Min
authors: M
authors: Zhang
authors: Center for Neuroimmunology and Regenerative Therapy, Shanghai Tenth People's Hospital, School of Medicine, Tongji University , Shanghai 200092, PR China.
authors: Dongsheng
authors: D
authors: Xu
authors: Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Jiangsu 215123, PR China.
authors: Guosheng
authors: G
authors: Cheng
mesh_terms: D002465:Cell Movement; D054377:Chemokine CXCL12; D006108:Graphite; D017628:Microglia; D058953:Neural Stem Cells; D019718:Receptors, CXCR4
chemical_list: D054377:Chemokine CXCL12; D019718:Receptors, CXCR4; D006108:Graphite
keywords: 3D graphene culture; SDF-1α/CXCR4; cell adhesion; microglia; migration