title: Immunolocalisation pattern of complex I-V in ageing human retina: Correlation with mitochondrial ultrastructure.
abstract: Earlier studies reported accumulation of mitochondrial DNA mutations in ageing and age-related macular degeneration. To know about the mitochondrial status with age, we examined immunoreactivity (IR) to markers of mitochondria (anti-mitochondrial antibody and voltage-dependent anion channel-1) and complex I-V (that mediate oxidative phosphorylation, OXPHOS) in donor human retinas (age: 19-94years; N=26; right eyes). In all samples, at all ages, IR to anti-mitochondrial antibody and voltage-dependent anion channel-1 was prominent in photoreceptor cells. Between second and seventh decade of life, strong IR to complex I-V was present in photoreceptors over macular to peripheral retina. With progressive ageing, the photoreceptors showed a decrease in complex I-IR (subunit NDUFB4) at eighth decade, and a weak or absence of IR in 10 retinas between ninth and tenth decade. Patchy IR to complex III and complex IV was detected at different ages. IR to ND1 (complex I) and complex II and V remained unaltered with ageing. Nitrosative stress (evaluated by IR to a nitro-tyrosine antibody) was found in photoreceptors. Superoxide dismutase-2 was found upregulated in photoreceptors with ageing. Mitochondrial ultrastructure was examined in two young retinas with intact complex IR and six aged retinas whose counterparts showed weak to absence of IR. Observations revealed irregular, photoreceptor inner segment mitochondria in aged maculae and mid-peripheral retina between eighth and ninth decade; many cones possessed autophagosomes with damaged mitochondria, indicating age-related alterations. A trend in age-dependent reduction of complex I-IR was evident in aged photoreceptors, whereas patchy complex IV-IR (subunits I and II) was age-independent, suggesting that the former is prone to damage with ageing perhaps due to oxidative stress. These changes in OXPHOS system may influence the energy budget of human photoreceptors, affecting their viability.
authors: Department of Anatomy, Neurobiology Laboratory, All India Institute of Medical Sciences, New Delhi 110029, India. Electronic address: tapas_nag@yahoo.com.
authors: Tapas Chandra
authors: TC
authors: Nag
authors: Department of Anatomy, Neurobiology Laboratory, All India Institute of Medical Sciences, New Delhi 110029, India.
authors: Shashi
authors: S
authors: Wadhwa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D045222:Electron Transport Chain Complex Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000737:chemistry* / Q000648:ultrastructure*; D010786:Photoreceptor Cells / Q000737:chemistry*; D012160:Retina / Q000737:chemistry*; D055815:Young Adult
chemical_list: D045222:Electron Transport Chain Complex Proteins
keywords: Ageing; Complex I; Mitochondria; Oxidative phosphorylation; Photoreceptors; Retina