title: Prophylactic Role of Oral Melatonin Administration on Neurogenesis in Adult Balb/C Mice during REM Sleep Deprivation.
abstract: Purpose. The aim of this study was to assess the effect of melatonin in the proliferation of neural progenitors, melatonin concentration, and antiapoptotic proteins in the hippocampus of adult mice exposed to 96 h REM sleep deprivation (REMSD) prophylactic administration of melatonin for 14 days. Material and Methods. Five groups of Balb/C mice were used: (1) control, (2) REMSD, (3) melatonin (10 mg/kg) plus REMSD, (4) melatonin and intraperitoneal luzindole (once a day at 5 mg/kg) plus REMSD, and (5) luzindole plus REMSD. To measure melatonin content in hippocampal tissue we used HPLC. Bcl-2 and Bcl-xL proteins were measured by Western Blot and neurogenesis was determined by injecting 5-bromo-2-deoxyuridine (BrdU) and BrdU/nestin expressing cells in the subgranular zone of the dentate gyrus were quantified by epifluorescence. Results. The melatonin-treated REMSD group showed an increased neural precursor in 44% with respect to the REMSD group and in 28% when contrasted with the control group (P < 0.021). The melatonin-treated REMSD group also showed the highest expression of Bcl-2 and Bcl-xL as compared to the rest of the groups. Conclusion. The exogenous administration of melatonin restores the tissue levels of sleep-deprived group and appears to be an efficient neuroprotective agent against the deleterious effects of REMSD.
authors: Laboratorio de Microscopía de Alta Resolución, Departamento de Neurociencias, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Calle Sierra Mojada 950, 44340 Guadalajara, JAL, Mexico; Centro de Enseñanza Técnico Industrial (CETI) Campus Colomos, Ingeniería en Mecatrónica-Biomédica, Calle Nueva Escocia 1885, 44638 Guadalajara, JAL, Mexico.
authors: Gabriela
authors: 0000-0001-8571-8764
authors: G
authors: López-Armas
authors: Departamento de Farmacobiología, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Boulevard Marcelino García Barragán 1421, 44430 Guadalajara, JAL, Mexico.
authors: Mario Eduardo
authors: ME
authors: Flores-Soto
authors: Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social (IMSS), Calle Sierra Mojada 800, 44340 Guadalajara, JAL, Mexico.
authors: Verónica
authors: V
authors: Chaparro-Huerta
authors: Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social (IMSS), Calle Sierra Mojada 800, 44340 Guadalajara, JAL, Mexico.
authors: Luis Felipe
authors: 0000-0001-6209-5031
authors: LF
authors: Jave-Suarez
authors: Laboratorio de Microscopía de Alta Resolución, Departamento de Neurociencias, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Calle Sierra Mojada 950, 44340 Guadalajara, JAL, Mexico.
authors: Sofía
authors: S
authors: Soto-Rodríguez
authors: Instituto de Biotecnología, Centro de Investigación Biomédica, Parque Tecnológico de Ciencias de la Salud, Universidad de Granada, Avenida del Conocimiento s/n, Armilla, 18100 Granada, Spain.
authors: Iryna
authors: 0000-0002-3640-7350
authors: I
authors: Rusanova
authors: Instituto de Biotecnología, Centro de Investigación Biomédica, Parque Tecnológico de Ciencias de la Salud, Universidad de Granada, Avenida del Conocimiento s/n, Armilla, 18100 Granada, Spain.
authors: Dario
authors: 0000-0002-9680-1560
authors: D
authors: Acuña-Castroviejo
authors: Laboratorio de Neurociencias, Facultad de Psicología, Universidad de Colima, Avenida Universidad 333, 28040 Colima, COL, Mexico.
authors: Oscar
authors: O
authors: González-Perez
authors: Laboratorio de Microscopía de Alta Resolución, Departamento de Neurociencias, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Calle Sierra Mojada 950, 44340 Guadalajara, JAL, Mexico.
authors: Rocío Elizabeth
authors: 0000-0001-6220-5456
authors: RE
authors: González-Castañeda
mesh_terms: D000284:Administration, Oral; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000008:administration & dosage / Q000493:pharmacokinetics; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D008550:Melatonin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D058953:Neural Stem Cells / Q000187:drug effects* / Q000473:pathology; D055495:Neurogenesis / Q000187:drug effects*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011897:Random Allocation; D012892:Sleep Deprivation / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D012895:Sleep, REM / Q000187:drug effects*; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: C494811:BCL2L1 protein, human; D002492:Central Nervous System Depressants; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein; D008550:Melatonin