title: MiR-200c promotes bladder cancer cell migration and invasion by directly targeting RECK.
abstract: BACKGROUND
  Increasing evidence suggests that the dysregulation of certain microRNAs plays an important role in tumorigenesis and metastasis. MiR-200c exhibits a disordered expression in many tumors and presents dual roles in bladder cancer (BC). Therefore, the definite role of miR-200c in BC needs to be investigated further.
  
  
  METHODS
  Quantitative reverse transcription polymerase chain reaction was used to assess miR-200c expression. Cell invasion and migration were evaluated using wound healing and transwell assays. The luciferase reporter assay was used to identify the direct target of miR-200c. The expression of reversion-inducing cysteine-rich protein with kazal motifs (RECK) in BC tissues and adjacent nontumor tissues, as well as in BC cell lines, was detected through quantitative reverse transcription polymerase chain reaction, Western blot assay, and immunohistochemistry.
  
  
  RESULTS
  The miR-200c expression was significantly upregulated in the BC tissues compared with the adjacent nontumor tissues. The downregulation of miR-200c significantly inhibited cell migration and invasion in the BC cell lines. The luciferase reporter assay showed that RECK was a direct target of miR-200c. The knockdown of RECK in the BC cell lines treated with anti-miR-200c elevated the previously attenuated cell migration and invasion.
  
  
  CONCLUSIONS
  Our findings indicated that miR-200c functions as oncogenes in BC and may provide a novel therapeutic strategy for the treatment of BC.
authors: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors: Yidong
authors: Y
authors: Cheng
authors: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors: Xiaolei
authors: X
authors: Zhang
authors: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors: Peng
authors: P
authors: Li
authors: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors: Chengdi
authors: C
authors: Yang
authors: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors: Jinyuan
authors: J
authors: Tang
authors: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors: Xiaheng
authors: X
authors: Deng
authors: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors: Xiao
authors: X
authors: Yang
authors: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors: Jun
authors: J
authors: Tao
authors: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors: Qiang
authors: Q
authors: Lu
authors: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
authors: Pengchao
authors: P
authors: Li
keywords: RECK; bladder cancer; invasion; miR-200c; migration