title: Non-specific activation of CD8α-characterised γδ T cells in PBL cultures of different chicken lines.
abstract: Avian γδ T lymphocytes still represent an enigmatic cell population not only regarding their functions but also their activation requirements and subset differentiation. To find a suitable method for non-specific stimulation and multiplication of CD8α-characterised γδ T-cell subsets in peripheral blood lymphocyte (PBL) cultures, PBLs of four different chicken lines (WLA, BLA, R11, L68) were treated with a range of commonly used non-specific reagents (PMA, PHA, ConA) and interleukins (IL-2, IL-12, IL-15), and the CD8α(-), CD8α(hi)β(+) and CD8αα(hi+) γδ T-cell subsets examined in relation to activation (CD25 expression) and proliferation by flow cytometry. The culture of avian PBLs with PMA led to an increase of CD25-expression intensity, whereas IL-2 induced proliferation of γδ T-cells. The combinational application of IL-2 plus PMA functioned synergistically and resulted in significantly enhanced numbers of CD25(+) cells, with simultaneous significant increase of the CD25-expression intensity and proliferation in all γδ T-cell subsets of all chicken lines. The four chicken lines revealed only sporadically differences with frequently highest proliferation rates in PBLs of WLA. In conclusion, PMA in combination with IL-2 is the best suitable additive for avian PBL cultures in order to multiply, activate and maintain CD8α-characterised γδ T-cell subsets of different chicken lines.
authors: Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut, Jena, Germany.
authors: Christina
authors: C
authors: Polasky
authors: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Neustadt-Mariensee, Germany.
authors: Steffen
authors: S
authors: Weigend
authors: Institute of Animal Welfare and Animal Husbandry, Friedrich-Loeffler-Institut, Celle, Germany.
authors: Lars
authors: L
authors: Schrader
authors: Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut, Jena, Germany. Electronic address: angela.berndt@fli.de.
authors: Angela
authors: A
authors: Berndt
mesh_terms: D000818:Animals; D016827:CD8 Antigens / Q000276:immunology*; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology*; D005434:Flow Cytometry; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D007376:Interleukin-2; D016692:Receptors, Antigen, T-Cell, gamma-delta; D013755:Tetradecanoylphorbol Acetate
keywords: CD25; Chicken lines; Non-specific activation; Proliferation; γδ T cells