title: Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients.
abstract: OBJECTIVE
  To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients.
  
  
  METHODS
  Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls). Gene expression analysis using quantitative polymerase chain reaction (qPCR) array was performed to compare (1) TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5) TKB versus TKC, and (6) UKB versus TKC.
  
  
  RESULTS
  Comparison 1 showed one down-regulated and one up-regulated gene; comparisons 2 and 3 resulted in the same five down-regulated genes; comparison 4 had no significant difference in relative gene expression; comparison 5 showed 26 down-regulated and 7 up-regulated genes; and comparison 6 showed 25 down-regulated and 11 up-regulated genes.
  
  
  CONCLUSIONS
  There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor.
authors: Fellow MSc degree, Division of Plastic Surgery, Department of Surgery, Universidade Federal de São Paulo (UNIFESP0, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, technical procedures, manuscript writing.
authors: Verônica
authors: V
authors: Chomiski
authors: PhD, Associate Professor, Division of Plastic Surgery, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. Conception, design, intellectual and scientific content of the study; analysis and interpretation of data; manuscript writing; critical revision.
authors: Alfredo
authors: A
authors: Gragnani
authors: Fellow MSc degree, Division of Plastic Surgery, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, technical procedures.
authors: Jéssica
authors: J
authors: Bonucci
authors: PhD, Postdoctoral degree, Division of Plastic Surgery, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, technical procedures, acquisition of data, manuscript writing.
authors: Silvana Aparecida Alves
authors: SA
authors: Correa
authors: PhD, Postdoctoral degree, Division of Plastic Surgery, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, manuscript writing.
authors: Samuel Marcos Ribeiro de
authors: SM
authors: Noronha
authors: Head, Full Professor, Division of Plastic Surgery, UNIFESP, Researcher 1A-CNPq, Director Medicine III-CAPES, Sao Paulo-SP, Brazil. Intellectual and scientific content of the study.
authors: Lydia Masako
authors: LM
authors: Ferreira
mesh_terms: D000328:Adult; D000818:Animals; D002056:Burns / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D002478:Cells, Cultured; D015536:Down-Regulation; D005260:Female; D051523:Fibroblast Growth Factor 7 / Q000494:pharmacology*; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D016133:Polymerase Chain Reaction; D012867:Skin / Q000166:cytology; D014945:Wound Healing / Q000235:genetics*
chemical_list: D051523:Fibroblast Growth Factor 7