title: Analysis of structures and epitopes of a novel secreted protein MYR1 in Toxoplasma gondii.
abstract: Toxoplasma gondii (Nicolle et Manceaux, 1908) is an obligate intracellular apicomplexan parasite and can infect warmblooded animals and humans all over the world. Development of effective vaccines is considered the only ideal way to control infection with T. gondii. However, only one live vaccine is commercially available for use in sheep and goats. Thus more effective antigenic proteins are searched for. In the present study we report a novel protein by secreted T. gondii termed Myc regulation 1 (MYR1). The physical and chemical characteristics, epitopes, hydrophilicity and functional sites of MYR1 were analysed by multiple bioinformatic approaches. The 3D models of MYR1 proteins were constructed and analysed. Furthermore, liner B-cell epitopes and T-cell epitopes of MYR1 protein and SAG1 were predicted. Compared to SAG1, MYR1 with good B-cell epitopes and T-cell epitopes had a potentiality to become a more successful vaccine against T. gondii. The bioinformatics analysis of MYR1 proteins could laid the foundation for further studies of its biological function experimentally and provide valuable information necessary for a better prevention and treatment of toxoplasmosis.
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, China.
authors: Jian
authors: J
authors: Zhou
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, China.
authors: Gang
authors: G
authors: Lu
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, China.
authors: Shenyi
authors: S
authors: He
mesh_terms: D000818:Animals; D000939:Epitopes / Q000378:metabolism*; D006801:Humans; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism*; D016052:Protozoan Vaccines / Q000276:immunology; D014122:Toxoplasma / Q000737:chemistry* / Q000378:metabolism*; D014123:Toxoplasmosis / Q000517:prevention & control / Q000628:therapy
chemical_list: D000939:Epitopes; D015800:Protozoan Proteins; D016052:Protozoan Vaccines
keywords: Myc regulation; PTM sites; bioinformatic analysis; toxoplasmosis; vaccine