title: Role of intestinal flora imbalance in pathogenesis of pouchitis.
abstract: OBJECTIVE
  To discuss the role of intestinal flora imbalance in the pathogenesis of pouchitis.
  
  
  METHODS
  The pouchitis rat model was established and the faeces sample and the mucous membrane sample were collected regularly, in which the bacterial nucleic acids were extracted for quantitative analysis of the intestinal flora in the samples through using the real-time quantitative PCR technique and high energy sequencing technology.
  
  
  RESULTS
  The disorder phenomenon of the intestinal flora appeared at the 7th day of the experiment, and the pouchitis was presented at the 21st day of the experiment. At the 31st day of the experiment, compared to control group and non-pouchitis group, the quantity of Bifidobacterium and the Lactobacillus of the pouchitis model rats in the mucous membrane sample and the faeces sample were significantly decreased (P < 0.05), and the Bacteroidetes, Faecalibacterium prausnitzii and XIV Clostridium leptum subgroup in the mucous membrane of pouchitis were significantly decreased (P < 0.05). The IV Clostridium coccoides group was the main flora in the mucous membrane of pouchitis, the bacterial diversity of non-pouchitis group and control group was significantly higher than that of the pouchitis group (P < 0.05).
  
  
  CONCLUSIONS
  The intestinal flora imbalance is one of the factors that cause the incidence of the pouchitis; this study provides a clue of the pathogenesis and treatment direction of the intestinal inflammatory disease.
authors: Research Institute of General Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province 210002, People's Republic of China.
authors: Xiao-Bo
authors: XB
authors: Feng
authors: Research Institute of General Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province 210002, People's Republic of China.
authors: Jun
authors: J
authors: Jiang
authors: Research Institute of General Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province 210002, People's Republic of China.
authors: Min
authors: M
authors: Li
authors: Research Institute of General Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province 210002, People's Republic of China.
authors: Gang
authors: G
authors: Wang
authors: Research Institute of General Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province 210002, People's Republic of China.
authors: Jin-Wei
authors: JW
authors: You
authors: State Key Laboratory of Infectious Disease Diagnosis and Treatment, First Affiliated Hospital of Medical School of Zhejiang University, Hangzhou, Zhejiang Province 310003, China. Electronic address: zuojian4743@163.com.
authors: Jian
authors: J
authors: Zuo
keywords: Bacteroid; Bifidobacterium; Intestinal flora; Lactobacillus; Pouchitis