title: A nanobiosensor for the detection of arginase activity.
abstract: A nanobiosensor for arginase detection was designed and synthesized. It features a central dopamine-coated iron/iron oxide nanoparticle to which sulfonated cyanine 7.0 is tethered via a stable amide bond. Cyanine 5.5 is linked to the N-terminal of the peptide sequence GRRRRRRRG. Arginine (R) reacts to ornithine (O) in the presence of arginase. Based on calibration with commercially obtained arginase II, the limit of detection (LOD) is picomolar. It is noteworthy that the nanobiosensor for arginase detection does not show a fluorescence increase when incubated with the enzyme NO-reductase, which also uses arginase as substrate, but is indicative of an inflammatory response by the host to cancer and infections. Arginase activity was determined in a syngeneic mouse model for aggressive breast cancer (4T1 tumors in BALB/c mice). It was found that the arginase activity is systemically enhanced, but especially pronounced in the active tumor regions.
authors: Department of Chemistry, Kansas State University, Manhattan, KS, USA.
authors: Aruni P
authors: AP
authors: Malalasekera
authors: Department of Chemistry, Kansas State University, Manhattan, KS, USA.
authors: Hongwang
authors: H
authors: Wang
authors: Department of Chemistry, Kansas State University, Manhattan, KS, USA.
authors: Thilani N
authors: TN
authors: Samarakoon
authors: Department of Chemistry, Kansas State University, Manhattan, KS, USA.
authors: Dinusha N
authors: DN
authors: Udukala
authors: Department of Chemistry, Kansas State University, Manhattan, KS, USA.
authors: Asanka S
authors: AS
authors: Yapa
authors: Department of Chemistry, Kansas State University, Manhattan, KS, USA.
authors: Raquel
authors: R
authors: Ortega
authors: Department of Anatomy & Physiology, Kansas State University, Manhattan, KS, USA.
authors: Tej B
authors: TB
authors: Shrestha
authors: Department of Anatomy & Physiology, Kansas State University, Manhattan, KS, USA.
authors: Hamad
authors: H
authors: Alshetaiwi
authors: Department of Chemistry, Kansas State University, Manhattan, KS, USA.
authors: Emily J
authors: EJ
authors: McLaurin
authors: Department of Anatomy & Physiology, Kansas State University, Manhattan, KS, USA.
authors: Deryl L
authors: DL
authors: Troyer
authors: Department of Chemistry, Kansas State University, Manhattan, KS, USA. Electronic address: sbossman@ksu.edu.
authors: Stefan H
authors: SH
authors: Bossmann
mesh_terms: D000818:Animals; D001119:Arginase / Q000378:metabolism*; D001120:Arginine; D015374:Biosensing Techniques; D001943:Breast Neoplasms / Q000175:diagnosis / Q000201:enzymology; D053768:Metal Nanoparticles; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide; D009952:Ornithine
chemical_list: D009569:Nitric Oxide; D001120:Arginine; D009952:Ornithine; D001119:Arginase
keywords: Arginase detection; Iron/iron oxide nanoparticle-based nanoplatform; Posttranslational sensor; Quantitative fluorescence detection