title: Dental pulp mesenchymal stem/stromal cells labeled with iron sucrose release exosomes and cells applied intra-nasally migrate to intracerebral glioblastoma.
abstract: We report on a simple iron oxide (Venofer) labeling procedure of dental pulp mesenchymal stem cells (DP-MSCs) and DP-MSCs transduced with yeast cytosinedeaminase::uracilphosphoribosyltransferase (yCD::UPRT-DP-MSCs). Venofer is a drug approved for intravenous application to treat iron deficiency anemia in patients. Venofer labeling did not affect DP-MSCs or yCD::UPRT-DP-MSCs viability and growth kinetics. Electron microscopy of labeled cells showed internalized Venofer nanoparticles in endosomes. MRI relativity measurement of Venofer labeled DP-MSCs in a phantom arrangement revealed that 100 cells per 0.1 ml were still detectable. DP-MSCs or yCD::UPRT-DP-MSCs and the corresponding Venofer labeled cells release exosomes into conditional medium (CM). CM from yCD::UPRT-DP-MSCs in the presence of a prodrug 5-fluorocytosine caused tumor cell death in a dose dependent manner. Iron labeled DP-MSCs or yCD::UPRT-DP-MSCs sustained their tumor tropism in vivo; intra-nasally applied cells migrated and specifically engrafted orthotopic glioblastoma xenografts in rats.
authors: U
authors: U
authors: Altanerova
authors: K
authors: K
authors: Benejova
authors: V
authors: V
authors: Altanerova
authors: S
authors: S
authors: Tyciakova
authors: B
authors: B
authors: Rychly
authors: P
authors: P
authors: Szomolanyi
authors: F
authors: F
authors: Ciampor
authors: M
authors: M
authors: Cihova
authors: V
authors: V
authors: Repiska
authors: K
authors: K
authors: Ondicova
authors: B
authors: B
authors: Mravec
authors: C
authors: C
authors: Altaner
mesh_terms: D000281:Administration, Intranasal; D002465:Cell Movement; D049109:Cell Proliferation; D003782:Dental Pulp / Q000166:cytology*; D055354:Exosomes; D000077605:Ferric Oxide, Saccharated / Q000493:pharmacokinetics; D005909:Glioblastoma; D006801:Humans; D059630:Mesenchymal Stem Cells
chemical_list: D000077605:Ferric Oxide, Saccharated
keywords: dental pulp MSCs; intranasal application migration to intracerebral glioblastoma.; iron labeling; yCD::UPRT-exosomes