title: Production of d-lactate from sugarcane bagasse and corn stover hydrolysates using metabolic engineered Escherichia coli strains.
abstract: In this study, the lactogenic Escherichia coli strain JU15 was used and modified to produce d-lactate (d-LA) from plant hydrolysates with a minimal nutrient addition in pH controlled fermenters. Results showed that strain JU15 produces d-LA with high yield and productivity in laboratory simulated hydrolysate media and actual sugar cane bagasse hemicellulosic hydrolysate. Strain JU15 showed sequential carbon source utilization and acetic acid production. The l-lactic and acetic acid production pathways were deleted in JU15, resulting strain AV03 (JU15 ΔpoxB, ΔackA-pta, ΔmgsA), which showed simultaneous consumption of glucose and xylose and no acetic acid production in the simulated hydrolysate. The d-LA yield from hydrolysate sugars was close to 0.95gD-LA/gsugars in all cases. Our results show that d-LA can be produced from plant hydrolysates in simple batch fermentation processes with a high productivity using engineered E. coli strains at fermenter scales from 0.2 up to 10L.
authors: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Mor. 62250, Mexico.
authors: José
authors: J
authors: Utrilla
authors: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Mor. 62250, Mexico.
authors: Alejandra
authors: A
authors: Vargas-Tah
authors: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Mor. 62250, Mexico.
authors: Berenice
authors: B
authors: Trujillo-Martínez
authors: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Mor. 62250, Mexico.
authors: Guillermo
authors: G
authors: Gosset
authors: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca, Mor. 62250, Mexico. Electronic address: alfredo@ibt.unam.mx.
authors: Alfredo
authors: A
authors: Martinez
mesh_terms: D002482:Cellulose / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis*; D031786:Saccharum / Q000378:metabolism*; D003313:Zea mays / Q000378:metabolism*
chemical_list: D019344:Lactic Acid; D002482:Cellulose; C027433:bagasse; D005947:Glucose
keywords: Corn stover; Hemicellulosic hydrolysates; Lactogenic Escherichia coli; Sugar cane bagasse; d-Lactate