title: An electrochemical biosensor for the assay of alpha-fetoprotein-L3 with practical applications.
abstract: The detection of alpha-fetoprotein-L3 (AFP-L3) is of great importance for hepatocellular carcinoma (HCC) diagnosis, but remains unsatisfactory owing to poor sensitivity and complex operating steps. In this work, a simple and sensitive method has been proposed for the detection of AFP-L3. Firstly, biotinylated Lens culinaris agglutinin-integrated silver nanoparticles (B-LCA-AgNPs) is fabricated. Owing to the specific interaction between Lens culinaris agglutinin and AFP-L3, AFP-L3 can be detected directly through the electrochemical signal readout of AgNPs, avoiding separated steps used in clinical practice. Furthermore, after the recognition between B-LCA-AgNPs and AFP-L3, large amount of AgNPs can be gathered at the binding site through avidin-biotin interactions, which can amplify the signal. Therefore, detection of AFP-L3 can be sensitively achieved. Moreover, compared with the other approaches, this new method has a better linear correlation (25-15,000pg/mL) and a lower detection limit (12pg/mL). Also, the new method developed in this work has been demonstrated to have good stability and reproducibility for the assay of AFP-L3 in human serum samples, so, it may hold a great application prospect in clinical diagnostics.
authors: State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University, Nanjing 210093, PR China; Department of Laboratory Medicine, the Second Affiliated Hospital of Southeast University, Nanjing 210003, PR China.
authors: Jinlong
authors: J
authors: Li
authors: State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University, Nanjing 210093, PR China.
authors: Tao
authors: T
authors: Gao
authors: State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University, Nanjing 210093, PR China.
authors: Shiyu
authors: S
authors: Gu
authors: State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University, Nanjing 210093, PR China.
authors: Jun
authors: J
authors: Zhi
authors: State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University, Nanjing 210093, PR China.
authors: Jie
authors: J
authors: Yang
authors: State Key Laboratory of Pharmaceutical Biotechnology and Collaborative Innovation Center of Chemistry for Life Sciences, Department of Biochemistry, Nanjing University, Nanjing 210093, PR China; Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai 200444, PR China. Electronic address: genxili@nju.edu.cn.
authors: Genxi
authors: G
authors: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods*; D019921:Biotinylation; D006528:Carcinoma, Hepatocellular / Q000097:blood; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D008113:Liver Neoplasms / Q000097:blood; D053768:Metal Nanoparticles / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry*; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D037121:Plant Lectins; D000509:alpha-Fetoproteins; C033619:lentil lectin; D012834:Silver
keywords: AFP-L3; Electrochemical biosensor; Lens culinaris agglutinin