title: Cas-analyzer: an online tool for assessing genome editing results using NGS data.
abstract: Genome editing with programmable nucleases has been widely adopted in research and medicine. Next generation sequencing (NGS) platforms are now widely used for measuring the frequencies of mutations induced by CRISPR-Cas9 and other programmable nucleases. Here, we present an online tool, Cas-Analyzer, a JavaScript-based implementation for NGS data analysis. Because Cas-Analyzer is completely used at a client-side web browser on-the-fly, there is no need to upload very large NGS datasets to a server, a time-consuming step in genome editing analysis. Currently, Cas-Analyzer supports various programmable nucleases, including single nucleases and paired nucleases.
  
  
  
  Free access at http://www.rgenome.net/cas-analyzer/ CONTACT: sangsubae@hanyang.ac.kr or jskim01@snu.ac.krSupplementary information: Supplementary data are available at Bioinformatics online.
authors: Department of Chemistry, Hanyang University, Seoul, South Korea.
authors: Jeongbin
authors: J
authors: Park
authors: Center for Genome Engineering, Institute for Basic Science, Seoul, South Korea.|Department of Chemistry, Seoul National University, Seoul, South Korea and.
authors: Kayeong
authors: K
authors: Lim
authors: Center for Genome Engineering, Institute for Basic Science, Seoul, South Korea.|Department of Chemistry, Seoul National University, Seoul, South Korea and.
authors: Jin-Soo
authors: JS
authors: Kim
authors: Department of Chemistry, Hanyang University, Seoul, South Korea.|Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul, South Korea.
authors: Sangsu
authors: S
authors: Bae
mesh_terms: D064113:CRISPR-Cas Systems; D004720:Endonucleases / Q000378:metabolism; D000072669:Gene Editing / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D020407:Internet; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software
chemical_list: D004720:Endonucleases