title: Rapid quantitation of human epididymis protein 4 in human serum by amplified luminescent proximity homogeneous immunoassay (AlphaLISA).
abstract: A sensitive, rapid and homogeneous reaction measurement method for quantitation of human epididymis protein 4 (HE4) in human serum by amplified luminescent proximity homogeneous immunoassay (AlphaLISA) was described. Built on a sandwich-type immunoassay format, analytes in samples were captured by one biotinylated monoclonal antibody combining on the surface of streptavidin coated donor beads, and "sandwiched" by another monoclonal antibody coated on acceptor beads. The coefficient variations of the method were lower than 10%, and the recoveries were in the range of 90-110% for serum samples. A value of 0.88pmol/l was identified as the minimum detectable dose of the present method for HE4. Compared with the results from electrochemiluminescence immunoassay kit (Roche) in 170 serum samples, there was a satisfied correlation coefficient of 0.984. The present assay demonstrated high sensitivity, wider effective detection range and excellent reproducibility for quantitation of HE4 can be useful for early screening and prognosis evaluation of patients with ovarian cancer.
authors: Department of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors: Hui
authors: H
authors: Zhao
authors: Teaching and Scientific Research Center, School of Biotechnology, Southern Medical University, Guangzhou, China.
authors: Guanfeng
authors: G
authors: Lin
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, China.
authors: Tiancai
authors: T
authors: Liu
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, China.
authors: Junyu
authors: J
authors: Liang
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, China.
authors: Zhiqi
authors: Z
authors: Ren
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, China.
authors: Rongliang
authors: R
authors: Liang
authors: Teaching and Scientific Research Center, School of Biotechnology, Southern Medical University, Guangzhou, China.
authors: Baihong
authors: B
authors: Chen
authors: Department of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China. Electronic address: huangwenhua2009@139.com.
authors: Wenhua
authors: W
authors: Huang
authors: Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, China. Electronic address: wg@smu.edu.cn.
authors: Yingsong
authors: Y
authors: Wu
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D010051:Ovarian Neoplasms / Q000175:diagnosis*; D011506:Proteins / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry*; D000081102:WAP Four-Disulfide Core Domain Protein 2
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D011506:Proteins; D000081102:WAP Four-Disulfide Core Domain Protein 2; C569698:WFDC2 protein, human
keywords: Amplified luminescent proximity homogeneous immunoassay; Human epididymis protein 4; Ovarian cancer