title: Colistin-resistant Escherichia coli clinical isolate harbouring the mcr-1 gene in Ecuador.
abstract: Colistin resistance mediated by the mcr-1 gene has been reported worldwide, but to date not from the Andean region, South America. We report the first clinical isolate of Escherichia coli harbouring the mcr-1 gene in Ecuador. The strain was isolated from peritoneal fluid from a 14-year-old male with acute appendicitis, and subjected to molecular analysis. The minimum inhibitory concentration of colistin for the strain was 8 mg/ml and it was susceptible to carbapenems but resistant to tigecycline. The strain harboured mcr-1 and bla CTX-M-55 genes and was of sequence type 609. The recognition of an apparently commensal strain of E. coli harbouring mcr-1 serves as an alert to the presence in the region of this recently described resistance mechanism to one of the last line of drugs available for the treatment of multi-resistant Gram-negative infections.
authors: Unidad de Investigaciones en Biomedicina,Zurita & Zurita Laboratorios,Quito,Ecuador.
authors: D
authors: D
authors: Ortega-Paredes
authors: Unidad de Investigaciones en Biomedicina,Zurita & Zurita Laboratorios,Quito,Ecuador.
authors: P
authors: P
authors: Barba
authors: Unidad de Investigaciones en Biomedicina,Zurita & Zurita Laboratorios,Quito,Ecuador.
authors: J
authors: J
authors: Zurita
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001064:Appendicitis / Q000382:microbiology; D003091:Colistin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004484:Ecuador; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; C000614037:MCR-1 protein, E coli; D003091:Colistin
keywords: 
                 Escherichia coli
              ; 
                 mcr-1
              ; Ecuador