title: Genetic code expansion in the mouse brain.
abstract: Site-specific incorporation of non-natural amino acids into proteins, via genetic code expansion with pyrrolysyl tRNA synthetase (PylRS) and tRNA(Pyl)CUA pairs (and their evolved derivatives) from Methanosarcina sp., forms the basis of powerful approaches to probe and control protein function in cells and invertebrate organisms. Here we demonstrate that adeno-associated viral delivery of these pairs enables efficient genetic code expansion in primary neuronal culture, organotypic brain slices and the brains of live mice.
authors: Medical Research Council Laboratory of Molecular Biology, Cambridge, England, UK.
authors: Russell J
authors: RJ
authors: Ernst
authors: Medical Research Council Laboratory of Molecular Biology, Cambridge, England, UK.
authors: Toke P
authors: TP
authors: Krogager
authors: Medical Research Council Laboratory of Molecular Biology, Cambridge, England, UK.
authors: Elizabeth S
authors: ES
authors: Maywood
authors: Medical Research Council Laboratory of Molecular Biology, Cambridge, England, UK.
authors: Roberto
authors: R
authors: Zanchi
authors: Medical Research Council Laboratory of Molecular Biology, Cambridge, England, UK.
authors: Václav
authors: V
authors: Beránek
authors: Medical Research Council Laboratory of Molecular Biology, Cambridge, England, UK.
authors: Thomas S
authors: TS
authors: Elliott
authors: Medical Research Council Laboratory of Molecular Biology, Cambridge, England, UK.
authors: Nicholas P
authors: NP
authors: Barry
authors: Medical Research Council Laboratory of Molecular Biology, Cambridge, England, UK.
authors: Michael H
authors: MH
authors: Hastings
authors: Medical Research Council Laboratory of Molecular Biology, Cambridge, England, UK.
authors: Jason W
authors: JW
authors: Chin
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism*; D000229:Dependovirus / Q000235:genetics; D005815:Genetic Code / Q000235:genetics*; D017020:Methanosarcina / Q000235:genetics; D051379:Mice; D015394:Molecular Structure; D012343:RNA, Transfer / Q000235:genetics* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases