title: Decreased demand for olfactory periglomerular cells impacts on neural precursor cell viability in the rostral migratory stream.
abstract: The subventricular zone (SVZ) provides a constant supply of new neurons to the olfactory bulb (OB). Different studies have investigated the role of olfactory sensory input to neural precursor cell (NPC) turnover in the SVZ but it was not addressed if a reduced demand specifically for periglomerular neurons impacts on NPC-traits in the rostral migratory stream (RMS). We here report that membrane type-1 matrix metalloproteinase (MT1-MMP) deficient mice have reduced complexity of the nasal turbinates, decreased sensory innervation of the OB, reduced numbers of olfactory glomeruli and reduced OB-size without alterations in SVZ neurogenesis. Large parts of the RMS were fully preserved in MT1-MMP-deficient mice, but we detected an increase in cell death-levels and a decrease in SVZ-derived neuroblasts in the distal RMS, as compared to controls. BrdU-tracking experiments showed that homing of NPCs specifically to the glomerular layer was reduced in MT1-MMP-deficient mice in contrast to controls while numbers of tracked cells remained equal in other OB-layers throughout all experimental groups. Altogether, our data show the demand for olfactory interneurons in the glomerular layer modulates cell turnover in the RMS, but has no impact on subventricular neurogenesis.
authors: Cellular Neurosciences, Max Delbrück Centre for Molecular Medicine in the Helmholtz Association, Robert Rössle Str. 10, Berlin, Germany.|Department of Psychiatry and Psychotherapy/Neuropsychiatry, Charité - Universitätsmedizin Berlin, Charitéplatz 1, Berlin, Germany.
authors: Anika
authors: A
authors: Langenfurth
authors: Neurosurgical Research, Ludwig Maximilians University Munich, Marchioninistr. 15, Munich, Germany.|Department of Neurosurgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China, 230022.
authors: Song
authors: S
authors: Gu
authors: Institute for Physiology, University of Hohenheim, Garbenstr. 30, Hohenheim, Germany.
authors: Verena
authors: V
authors: Bautze
authors: Neurosurgical Research, Ludwig Maximilians University Munich, Marchioninistr. 15, Munich, Germany.
authors: Caiyi
authors: C
authors: Zhang
authors: Center for Neuropathology, Ludwig Maximilians University Munich, Feodor-Lynen-Str. 23, Munich, Germany.|Institute of Neuropathology, University Medical Center, Hamburg-Eppendorf, Germany.|Research Institute Children's Cancer Center, Hamburg, Germany.
authors: Julia E
authors: JE
authors: Neumann
authors: Center for Neuropathology, Ludwig Maximilians University Munich, Feodor-Lynen-Str. 23, Munich, Germany.|Institute of Neuropathology, University Medical Center, Hamburg-Eppendorf, Germany.|Research Institute Children's Cancer Center, Hamburg, Germany.|Department of Pediatric Hematology and Oncology, University Medical Center Hamburg-Eppendorf, 20264 Hamburg, Germany.
authors: Ulrich
authors: U
authors: Schüller
authors: Cellular Neurosciences, Max Delbrück Centre for Molecular Medicine in the Helmholtz Association, Robert Rössle Str. 10, Berlin, Germany.
authors: Kristin
authors: K
authors: Stock
authors: Cellular Neurosciences, Max Delbrück Centre for Molecular Medicine in the Helmholtz Association, Robert Rössle Str. 10, Berlin, Germany.
authors: Susanne A
authors: SA
authors: Wolf
authors: Institute for Physiology, University of Hohenheim, Garbenstr. 30, Hohenheim, Germany.
authors: Anna-Maria
authors: AM
authors: Maier
authors: Neurosurgical Research, Ludwig Maximilians University Munich, Marchioninistr. 15, Munich, Germany.
authors: Giorgia
authors: G
authors: Mastrella
authors: Craniofacial and Skeletal Diseases Branch, NIDCR, National Institutes of Health (NIH), Bethesda, Maryland 20892-4380, USA.
authors: Andrew
authors: A
authors: Pak
authors: Department of Neurosurgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China, 230022.
authors: Hongwei
authors: H
authors: Cheng
authors: Neurosurgical Research, Ludwig Maximilians University Munich, Marchioninistr. 15, Munich, Germany.
authors: Roland E
authors: RE
authors: Kälin
authors: Craniofacial and Skeletal Diseases Branch, NIDCR, National Institutes of Health (NIH), Bethesda, Maryland 20892-4380, USA.
authors: Kenn
authors: K
authors: Holmbeck
authors: Institute for Physiology, University of Hohenheim, Garbenstr. 30, Hohenheim, Germany.
authors: Jörg
authors: J
authors: Strotmann
authors: Cellular Neurosciences, Max Delbrück Centre for Molecular Medicine in the Helmholtz Association, Robert Rössle Str. 10, Berlin, Germany.
authors: Helmut
authors: H
authors: Kettenmann
authors: Neurosurgical Research, Ludwig Maximilians University Munich, Marchioninistr. 15, Munich, Germany.
authors: Rainer
authors: R
authors: Glass
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D020547:Lateral Ventricles / Q000166:cytology / Q000378:metabolism*; D053511:Matrix Metalloproteinase 14 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D009830:Olfactory Bulb / Q000166:cytology / Q000378:metabolism*; D018034:Olfactory Receptor Neurons / Q000166:cytology / Q000378:metabolism*
chemical_list: C507096:Mmp14 protein, mouse; D053511:Matrix Metalloproteinase 14