title: The mitochondrial BKCachannel cardiac interactome reveals BKCaassociation with the mitochondrial import receptor subunit Tom22, and the adenine nucleotide translocator.
abstract: Mitochondrial BKCachannel, mitoBKCa, regulates mitochondria function in the heart but information on its protein partnerships in cardiac mitochondria is missing. A directed proteomic approach discovered the novel interaction of BKCawith Tom22, a component of the mitochondrion outer membrane import system, and the adenine nucleotide translocator (ANT). The expressed protein partners co-immunoprecipitated and co-segregated into mitochondrial fractions in HEK293T cells. The BKCa50 amino acid splice insert, DEC, facilitated BKCainteraction with ANT. Further, BKCatransmembrane domain was required for the association with both Tom22 and ANT. The results serve as a working framework to understand mitoBKCaimport and functional relationships.
authors: Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Department of Molecular & Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA.
authors: Jin
authors: J
authors: Zhang
authors: Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
authors: Min
authors: M
authors: Li
authors: Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
authors: Zhu
authors: Z
authors: Zhang
authors: Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
authors: Ronghui
authors: R
authors: Zhu
authors: Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Department of Physiology, University of California, Los Angeles, Los Angeles, CA, USA; Brain Research Institute, University of California, Los Angeles, Los Angeles, CA, USA; Cardiovascular Research Laboratory, University of California, Los Angeles, Los Angeles, CA, USA.
authors: Riccardo
authors: R
authors: Olcese
authors: Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Department of Physiology, University of California, Los Angeles, Los Angeles, CA, USA; Brain Research Institute, University of California, Los Angeles, Los Angeles, CA, USA; Cardiovascular Research Laboratory, University of California, Los Angeles, Los Angeles, CA, USA.
authors: Enrico
authors: E
authors: Stefani
authors: Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Department of Molecular & Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, USA; Brain Research Institute, University of California, Los Angeles, Los Angeles, CA, USA; Cardiovascular Research Laboratory, University of California, Los Angeles, Los Angeles, CA, USA. Electronic address: ltoro@ucla.edu.
authors: Ligia
authors: L
authors: Toro
mesh_terms: D000818:Animals; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000378:metabolism*; D008297:Male; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology*; D011485:Protein Binding; D060066:Protein Interaction Maps; D040901:Proteomics; D017207:Rats, Sprague-Dawley
chemical_list: D051036:Large-Conductance Calcium-Activated Potassium Channels; D033681:Mitochondrial Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D024101:Mitochondrial Proteins; C489971:TOMM22 protein, human; D000226:Mitochondrial ADP, ATP Translocases
keywords: Adenine nucleotide translocator; BK channel; Heart; Macromolecular complex; MaxiK channel; Mitochondria; Potassium ion channel; Proteomics; Tom22