title: Fabrication of type I collagen microcarrier using a microfluidic 3D T-junction device and its application for the quantitative analysis of cell-ECM interactions.
abstract: We presented a new quantitative analysis for cell and extracellular matrix (ECM) interactions, using cell-coated ECM hydrogel microbeads (hydrobeads) made of type I collagen. The hydrobeads can carry cells as three-dimensional spheroidal forms with an ECM inside, facilitating a direct interaction between the cells and ECM. The cells on hydrobeads do not have a hypoxic core, which opens the possibility for using as a cell microcarrier for bottom-up tissue reconstitution. This technique can utilize various types of cells, even MDA-MB-231 cells, which have weak cell-cell interactions and do not form spheroids in conventional spheroid culture methods. Morphological indices of the cell-coated hydrobead visually present cell-ECM interactions in a quantitative manner.
authors: School of Mechanical Engineering, Korea University, 145 Anam-ro, Seoungbuk-gu, Seoul 02841, Korea.
authors: Junghyo
authors: J
authors: Yoon
authors: Jaehoon
authors: J
authors: Kim
authors: Hyo Eun
authors: HE
authors: Jeong
authors: Ryo
authors: R
authors: Sudo
authors: Myung-Jin
authors: MJ
authors: Park
authors: Seok
authors: S
authors: Chung
mesh_terms: D015820:Cadherins / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002460:Cell Line; D002470:Cell Survival; D024042:Collagen Type I / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D046210:Microfluidic Analytical Techniques / Q000379:methods; D008856:Microscopy, Fluorescence; D014783:Viscosity
chemical_list: D015820:Cadherins; D024042:Collagen Type I; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate