title: Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening.
abstract: RNAi has now become a valuable research tool for cell-based high-throughput screening. However, traditional RNAi high-throughput methods are based on multi-well plates, relying on expensive instruments and complicated operations. In this chapter, we describe a method termed self-assembled cell microarray (SAMcell), which integrates micro-fabrication, reverse transfection, and RNAi technologies and allows for cell behavior investigations to be performed directly on the cell chip. This method has been successfully employed to perform large-scale functional screening assays to identify gene modulators of cell migration, cell proliferation, and cellular apoptosis.
authors: Biomedical Engineering Department, College of Engineering, Peking University, Beijing, 100871, China. shzzhshuo@sina.com.
authors: Hanshuo
authors: H
authors: Zhang
authors: Suzhou Genoarray Biotech Co. Ltd., Suzhou, 215123, China.
authors: Juan
authors: J
authors: Li
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D006367:HeLa Cells; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D034622:RNA Interference; D046888:Tissue Array Analysis / Q000295:instrumentation / Q000379:methods*; D014162:Transfection / Q000379:methods
keywords: Cell behavior; Chip; High-throughput screening; RNAi; SAMcell