title: Pistricoccus aurantiacus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a shark.
abstract: A novel Gram-stain negative, non-motile, moderately halophilic, facultatively anaerobic and spherical bacterium designated strain SS9Twas isolated from the gill homogenate of a shark. Cells of SS9Twere observed to be 0.8-1.2 μm in diameter. The strain was found to grow optimally at 33 °C, pH 7.0-8.0 and in the presence of 6.0 % (w/v) NaCl. On the basis of 16S rRNA gene phylogeny, strain SS9Tcan be affiliated with the family Halomonadaceae and is closely related to Chromohalobacter marismortui NBRC 103155T(95.6 % sequence similarity), Halomonas ilicicola SP8T(95.6 %) and Chromohalobacter salexigens DSM 3043T(95.5 %). Multilocus sequence analysis of strain SS9Tusing the housekeeping genes 16S rRNA, 23S rRNA, gyrB, rpoD and secA revealed the strain's distinct phylogenetic position, separate from other known genera of the family Halomonadaceae. Strain SS9Twas found to contain ubiquinone-9 (Q-9) as the predominant ubiquinone and C18:1ω7c, C16:0and summed feature 3 (C16:1ω7c and/or iso-C15:02-OH) as the major fatty acids. The major polar lipids of strain SS9Twere identified as phosphatidylglycerol and phosphatidylethanolamine. The DNA G + C content of strain SS9Twas determined to be 60.4 mol%. It is evident from phylogenetic, genotypic, phenotypic and chemotaxonomic results that strain SS9Trepresents a novel species in a new genus, for which the name Pistricoccus aurantiacus gen. nov., sp. nov. is proposed. The type strain is SS9T(=KCTC 42586T= MCCC 1H00111T).
authors: College of Marine Science, Shandong University at Weihai, Weihai, 264209, China.
authors: Zhen-Xing
authors: ZX
authors: Xu
authors: College of Marine Science, Shandong University at Weihai, Weihai, 264209, China.
authors: Qi-Yun
authors: QY
authors: Liang
authors: College of Marine Science, Shandong University at Weihai, Weihai, 264209, China.
authors: De-Chen
authors: DC
authors: Lu
authors: College of Marine Science, Shandong University at Weihai, Weihai, 264209, China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China.
authors: Guan-Jun
authors: GJ
authors: Chen
authors: College of Marine Science, Shandong University at Weihai, Weihai, 264209, China. duzongjun@sdu.edu.cn.|State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China. duzongjun@sdu.edu.cn.
authors: Zong-Jun
authors: ZJ
authors: Du
mesh_terms: D000818:Animals; D002681:China; D044129:Halomonadaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D058889:Molecular Typing; D010802:Phylogeny; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012754:Sharks / Q000382:microbiology*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene; MLSA; Moderately halophilic bacterium; Pistricoccus aurantiacus