title: An enzyme-free surface plasmon resonance biosensing strategy for detection of DNA and small molecule based on nonlinear hybridization chain reaction.
abstract: A label-free and enzyme-free surface plasmon resonance (SPR) biosensing strategy has been developed for highly sensitive and specific detection of target DNA by employing the nonlinear hybridization chain reaction (HCR) amplification. Nonlinear HCR is a hairpin-free system in which double-stranded DNA monomers could dendritically assemble into highly branched nanostructure upon introducing a trigger sequence. The target DNA partly hybridizes with capture probe on the gold sensing chip and the unpaired fragment of target DNA works as a trigger to initiate the nonlinear HCR, forming a chain-branching growth of DNA dendrimer by self-assembly. Real-time amplified SPR response is observed upon the introduction of nonlinear HCR system. The method is capable of detecting target DNA at the concentration down to 0.85 pM in 60min with a dynamic range from 1 pM to 1000 pM, and could discriminate target DNA from mismatched sequences. This biosensing strategy exhibits good reproducibility and precision, and has been successfully applied for detection of target DNA in complex sample matrices. In addition, the nonlinear HCR based SPR biosensing methodology is extended to the detection of adenosine triphosphate (ATP) by aptamer recognition. Thus, the versatile method might become a potential alternative tool for biomolecule detection in medical research and early clinical diagnosis.
authors: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors: Xiaojuan
authors: X
authors: Ding
authors: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China; Molecular Oncology and Epigenetics Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors: Wei
authors: W
authors: Cheng
authors: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors: Yujian
authors: Y
authors: Li
authors: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors: Jiangling
authors: J
authors: Wu
authors: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors: Xinmin
authors: X
authors: Li
authors: Department of Chemistry, University of California, Riverside, CA 92521, USA.
authors: Quan
authors: Q
authors: Cheng
authors: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. Electronic address: dingshijia@163.com.
authors: Shijia
authors: S
authors: Ding
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D015342:DNA Probes; D006046:Gold; D000255:Adenosine Triphosphate; D004247:DNA
keywords: Adenosine triphosphate; Biosensing strategy; DNA; Nonlinear hybridization chain reaction; Surface plasmon resonance