title: A novel chitosan-tussah silk fibroin/nano-hydroxyapatite composite bone scaffold platform with tunable mechanical strength in a wide range.
abstract: Currently, great efforts have been made to enhance the mechanical strength of bone tissue engineering (BTE) scaffolds, which are composed of biopolymeric matrices and inorganic nano-fillers. But the tunability of mechanical strength in a wide range for BTE scaffolds has seldom been investigated in spite of the great importance of this performance. In this work, a chitosan-tussah silk fibroin/hydroxyapatite (CS-TSF/HAp) hydrogel was synthesized by using a novel in situ precipitation method. Through in situ inducing the conformation transition of TSF in the CS-TSF/HAp hydrogel, which could be monitored by XRD, FT-IR, TGA, and DTA, the elastic modulus and fracture strength of the final CS-TSF/HAp composite could be tailored in a wide range without changing its composition, morphology, roughness, and crystal structures. The elastic modulus of the CS-TSF/HAp composite ranged from ∼250 to ∼400MPa while its fracture strength ranged from ∼45 to ∼100MPa. In order to clarify the rationale behind this process, a speculative explanation was provided. In vitro cell culture indicated that MC3T3-E1 cells cultured on the CS-TSF/HAp composite had positive adhesion, proliferation, and differentiation potential. We believed that the CS-TSF/HAp composite could be used as an ideal scaffold platform for cell culture and implantation of bone reconstruction.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
authors: Jiabing
authors: J
authors: Ran
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
authors: Jingxiao
authors: J
authors: Hu
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
authors: Guanglin
authors: G
authors: Sun
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
authors: Si
authors: S
authors: Chen
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
authors: Pei
authors: P
authors: Jiang
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China. Electronic address: shenxy@whu.edu.cn.
authors: Xinyu
authors: X
authors: Shen
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China. Electronic address: sem@whu.edu.cn.
authors: Hua
authors: H
authors: Tong
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials; D012831:Bombyx; D018786:Bone Substitutes; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D055119:Elastic Modulus; D005349:Fibroins / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D016062:Porosity; D013718:Tensile Strength; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D020100:Hydrogels; D005349:Fibroins; D048271:Chitosan; D017886:Durapatite
keywords: Bone tissue engineering; Mechanical strength; Tussah silk fibroin