title: Artificial Diels-Alderase based on the transmembrane protein FhuA.
abstract: Copper(I) and copper(II) complexes were covalently linked to an engineered variant of the transmembrane protein Ferric hydroxamate uptake protein component A (FhuA ΔCVF(tev)). Copper(I) was incorporated using an N-heterocyclic carbene (NHC) ligand equipped with a maleimide group on the side arm at the imidazole nitrogen. Copper(II) was attached by coordination to a terpyridyl ligand. The spacer length was varied in the back of the ligand framework. These biohybrid catalysts were shown to be active in the Diels-Alder reaction of a chalcone derivative with cyclopentadiene to preferentially give the endo product.
authors: Institute of Inorganic Chemistry, RWTH Aachen University, Landoltweg 1, 52056 Aachen, Germany.
authors: Hassan
authors: H
authors: Osseili
authors: Institute of Inorganic Chemistry, RWTH Aachen University, Landoltweg 1, 52056 Aachen, Germany.
authors: Daniel F
authors: DF
authors: Sauer
authors: Institute of Inorganic Chemistry, RWTH Aachen University, Landoltweg 1, 52056 Aachen, Germany.
authors: Klaus
authors: K
authors: Beckerle
authors: Institute of Biotechnology, RWTH Aachen University, Worringer Weg 1, 52056 Aachen, Germany.
authors: Marcus
authors: M
authors: Arlt
authors: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan.
authors: Tomoki
authors: T
authors: Himiyama
authors: Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany.
authors: Tino
authors: T
authors: Polen
authors: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan.
authors: Akira
authors: A
authors: Onoda
authors: Institute of Biotechnology, RWTH Aachen University, Worringer Weg 1, 52056 Aachen, Germany.
authors: Ulrich
authors: U
authors: Schwaneberg
authors: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan.
authors: Takashi
authors: T
authors: Hayashi
authors: Institute of Inorganic Chemistry, RWTH Aachen University, Landoltweg 1, 52056 Aachen, Germany.
authors: Jun
authors: J
authors: Okuda
keywords: artificial Diels-Alderase; biohybrid catalysis; copper enyzme; membrane protein