title: Functionalized poly (ionic liquid) as the support to construct a ratiometric electrochemical biosensor for the selective determination of copper ions in AD rats.
abstract: An efficient ratiometric electrochemical biosensor for Cu2+determination was constructed using dual hydroxyl-functionalized poly (ionic liquid) (DHF-PIL) as the catalyst support. The DHF-PIL exhibited typical macroporous structure, which provided a high surface area of 39.31m2/g for the sufficient loading of biomolecules. The specific recognition of Cu2+was accomplished by employing neurokinin B (NKB) for the first time, which could bind to Cu2+to form a [CuII(NKB)2] complex with high specificity. Meanwhile, a common redox mediator, 2, 2'-Azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) was modified into DHF-PIL by electrostatic interactions to act as an inner reference molecule, which provided a built-in correction for environmental effects and improving the detection accuracy. With this strategy, the developed electrochemical biosensor was capable of determining Cu2+with a linear range between 0.9 and 36.1μM and low detection limit (LOD) and quantification limit (LOQ) of 0.24 and 0.6μM, respectively. The sensor also displayed a satisfactory selectivity against a series of interferences in the brain, including metal ions, amino acids and other endogenous compounds. Accordingly, the present biosensor was successfully applied to evaluate Cu2+levels in normal and AD rats.
authors: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, Jiangsu 221004, PR China.
authors: Yanyan
authors: Y
authors: Yu
authors: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, Jiangsu 221004, PR China.
authors: Chao
authors: C
authors: Yu
authors: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, Jiangsu 221004, PR China.
authors: Tianxiao
authors: T
authors: Yin
authors: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, Jiangsu 221004, PR China.
authors: Shanshan
authors: S
authors: Ou
authors: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, Jiangsu 221004, PR China.
authors: Xiaoyu
authors: X
authors: Sun
authors: College of Biomedical Sciences, Xuzhou Medical University, 209 Tongshan Road, Xuzhou 221004, PR China.
authors: Xiangru
authors: X
authors: Wen
authors: Jiangsu Vcare Pharmatech Company, 15 Wanshou Road, Nanjing, Jiangsu 210000, PR China.
authors: Lin
authors: L
authors: Zhang
authors: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, Jiangsu 221004, PR China. Electronic address: tdq993@126.com.
authors: Daoquan
authors: D
authors: Tang
authors: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, Jiangsu 221004, PR China. Electronic address: yinxx@xzmc.edu.cn.
authors: Xiaoxing
authors: X
authors: Yin
mesh_terms: D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000473:pathology; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001923:Brain Chemistry; D002384:Catalysis; D003300:Copper / Q000032:analysis* / Q000134:cerebrospinal fluid*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D052578:Ionic Liquids / Q000737:chemistry*; D057230:Limit of Detection; D015287:Neurokinin B / Q000737:chemistry; D051381:Rats
chemical_list: D052578:Ionic Liquids; D003300:Copper; D015287:Neurokinin B
keywords: AD; Cu(2+); Electrochemistry; Functionalized PILs; Neurokinin B