title: Vibrational monitor of early demineralization in tooth enamel after in vitro exposure to phosphoridic liquid.
abstract: The Raman spectroscopic method has been applied to quantitatively assess the in vitro degree of demineralization in healthy human teeth. Based on previous evaluations of Raman selection rules (empowered by an orientation distribution function (ODF) statistical algorithm) and on a newly proposed analysis of phonon density of states (PDOS) for selected vibrational modes of the hexagonal structure of hydroxyapatite, a molecular-scale evaluation of the demineralization process upon in vitro exposure to a highly acidic beverage (i.e., CocaCola™ Classic, pH=2.5) could be obtained. The Raman method proved quite sensitive and spectroscopic features could be directly related to an increase in off-stoichiometry of the enamel surface structure since the very early stage of the demineralization process (i.e., when yet invisible to other conventional analytical techniques). The proposed Raman spectroscopic algorithm might possess some generality for caries risk assessment, allowing a prompt non-contact diagnostic practice in dentistry.
authors: Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, 606-8585 Kyoto, Japan; Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan. Electronic address: pezzotti@kit.ac.jp.
authors: Giuseppe
authors: G
authors: Pezzotti
authors: Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan.
authors: Tetsuya
authors: T
authors: Adachi
authors: Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, 606-8585 Kyoto, Japan; Department of Engineering and Architecture, University of Trieste, Via A. Valerio 6/A, 34127 Trieste, Italy.
authors: Isabella
authors: I
authors: Gasparutti
authors: Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, 606-8585 Kyoto, Japan; Chemistry, Material and Chemical Engineering Department "Giulio Natta", Polytechnic of Milan, Via Mancinelli 7, Milan 20131, Italy.
authors: Giulio
authors: G
authors: Vincini
authors: Department of Medical Engineering for Treatment of Bone and Joint Disorders, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0854, Japan.
authors: Wenliang
authors: W
authors: Zhu
authors: Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, 606-8585 Kyoto, Japan.
authors: Marco
authors: M
authors: Boffelli
authors: Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, 606-8585 Kyoto, Japan; Department of Engineering and Architecture, University of Trieste, Via A. Valerio 6/A, 34127 Trieste, Italy.
authors: Alfredo
authors: A
authors: Rondinella
authors: Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, 606-8585 Kyoto, Japan.
authors: Elia
authors: E
authors: Marin
authors: Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan.
authors: Hiroaki
authors: H
authors: Ichioka
authors: Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan.
authors: Toshiro
authors: T
authors: Yamamoto
authors: Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan; Department of Bio-Ionomics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan.
authors: Yoshinori
authors: Y
authors: Marunaka
authors: Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan.
authors: Narisato
authors: N
authors: Kanamura
mesh_terms: D002253:Carbonated Beverages / Q000009:adverse effects*; D003731:Dental Caries / Q000000981:diagnostic imaging; D003743:Dental Enamel / Q000737:chemistry* / Q000000981:diagnostic imaging / Q000187:drug effects*; D009063:Dental Physiological Phenomena / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D008963:Molar / Q000737:chemistry / Q000000981:diagnostic imaging*; D010756:Phosphoric Acids / Q000009:adverse effects; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties; D014732:Vibration
chemical_list: D010756:Phosphoric Acids; D017886:Durapatite; C030242:phosphoric acid
keywords: Demineralization; Raman spectroscopy; Tooth