title: Fatty acid ω-hydroxylases from Solanum tuberosum.
abstract: Potato StCYP86A33 complements the Arabidopsis AtCYP86A1 mutant, horst - 1. Suberin is a cell-wall polymer that comprises both phenolic and aliphatic components found in specialized plant cells. Aliphatic suberin is characterized by bi-functional fatty acids, typically ω-hydroxy fatty acids and α,ω-dioic acids, which are linked via glycerol to form a three-dimensional polymer network. In potato (Solanum tuberosum L.), over 65 % of aliphatics are either ω-hydroxy fatty acids or α,ω-dioic acids. Since the biosynthesis of α,ω-dioic acids proceeds sequentially through ω-hydroxy fatty acids, the formation of ω-hydroxy fatty acids represents a significant metabolic commitment during suberin deposition. Four different plant cytochrome P450 subfamilies catalyze ω-hydroxylation, namely, 86A, 86B, 94A, and 704B; though to date, only a few members have been functionally characterized. In potato, CYP86A33 has been identified and implicated in suberin biosynthesis through reverse genetics (RNAi); however, attempts to express the CYP86A33 protein and characterize its catalytic function have been unsuccessful. Herein, we describe eight fatty acid ω-hydroxylase genes (three CYP86As, one CYP86B, three CYP94As, and a CYP704B) from potato and demonstrate their tissue expression. We also complement the Arabidopsis cyp86A1 mutant horst-1 using StCYP86A33 under the control of the Arabidopsis AtCYP86A1 promoter. Furthermore, we provide preliminary analysis of the StCYP86A33 promoter using a hairy root transformation system to monitor pStCYP86A33::GUS expression constructs. These data confirm the functional role of StCYP86A33 as a fatty acid ω-hydroxylase, and demonstrate the utility of hairy roots in the study of root-specific genes.
authors: Department of Biology and the Biotron, The University of Western Ontario, London, ON, N6A 5B7, Canada.
authors: Anica
authors: A
authors: Bjelica
authors: Department of Biology and the Biotron, The University of Western Ontario, London, ON, N6A 5B7, Canada.
authors: Meghan L
authors: ML
authors: Haggitt
authors: Department of Biology and the Biotron, The University of Western Ontario, London, ON, N6A 5B7, Canada.
authors: Kathlyn N
authors: KN
authors: Woolfson
authors: Department of Biology and the Biotron, The University of Western Ontario, London, ON, N6A 5B7, Canada.
authors: Daniel P N
authors: DP
authors: Lee
authors: Department of Biology and the Biotron, The University of Western Ontario, London, ON, N6A 5B7, Canada.
authors: Abdullah B
authors: AB
authors: Makhzoum
authors: Department of Biology and the Biotron, The University of Western Ontario, London, ON, N6A 5B7, Canada. bernards@uwo.ca.
authors: Mark A
authors: MA
authors: Bernards
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D001483:Base Sequence; D042926:Cytochrome P-450 CYP4A / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005816:Genetic Complementation Test; D008055:Lipids / Q000737:chemistry; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D035281:Plant Tubers / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011198:Solanum tuberosum / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
chemical_list: D008055:Lipids; D010940:Plant Proteins; D000040:Abscisic Acid; C065875:suberin; D042926:Cytochrome P-450 CYP4A
keywords: CYP86A1; CYP86A33; Potato; Solanum tuberosum L.; Suberin; ω-Hydroxylases