title: Synchronization of S Phase in Schizosaccharomyces pombe Cells by Transient Exposure to M-Factor Pheromone.
abstract: A well-characterized S phase, a unicellular lifestyle, and a plethora of mutations in key components of DNA metabolism make fission yeast a particularly attractive system in which to study DNA replication. However, synchronization of passage through a normal S phase has proved challenging. This protocol describes how combining nitrogen starvation with M-factor mating pheromone treatment presents a highly effective method for synchronizing passage through an ostensibly normal S phase.
authors: Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
authors: Olaf
authors: O
authors: Nielsen
mesh_terms: D004261:DNA Replication; D010675:Pheromones / Q000378:metabolism*; D016196:S Phase / Q000187:drug effects*; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D010675:Pheromones