title: Gene sequencing and variable site analyzing of coding region of two enterovirus A71 isolates with different clinical phenotypes.
abstract: Currently, it is still controversial that if the pathogenicity of EV-A71 causing severe or mild hand, foot, and mouth disease (HFMD) is associated with viral nucleotide or amino acid sequence(s). In this study, 19 clinical strains were detected in samples from diagnosed patients of EV-A71-caused HFMD with mild or severe symptoms. Then, VP1-2A fragment sequences of 19 EV-A71 isolates were determined, the phylogenetic analysis, based on VP1 sequences of 19 EV-A71 stains in this study and which of 62 EV-A71 strains with different clinical phenotypes reported before, were carried out. Our results showed that no difference in the genotype and evolution distribution was observed among the EV-A71 strains mentioned above. Furthermore, two EV-A71 isolates, which with much close evolutionary relationship but different clinical manifestations, were purified by plaque assay, the complete genome sequencing was done, and deduced amino acid sequence analysis of 11 proteins coded by EV-A71 was carried out. Eight variable amino acid sites were found and further verified with those of 62 strains reported before. Our study provides further evidence that the potential pathogenicity of EV-A71 causing severe or mild HFMD seems not to be associated with viral genotype and even the amino acid substitution.
authors: Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.
authors: Bing-Yu
authors: BY
authors: Xie
authors: Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.
authors: Chao
authors: C
authors: Xu
authors: Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.
authors: Qing
authors: Q
authors: Xiong
authors: Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.
authors: Meng
authors: M
authors: Zhu
authors: Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.
authors: Hao
authors: H
authors: Duan
authors: Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.
authors: Yu-Ya
authors: YY
authors: Wang
authors: Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.
authors: Hao
authors: H
authors: Zhang
authors: Infectious Disease Center, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, People's Republic of China.
authors: Xue-Fei
authors: XF
authors: Ni
authors: Infectious Disease Center, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, People's Republic of China.
authors: Ling-Hang
authors: LH
authors: Wang
authors: Infectious Disease Center, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, People's Republic of China.
authors: Xing-Wang
authors: XW
authors: Li
authors: Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.
authors: Tao
authors: T
authors: Shen
authors: Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China. Electronic address: ypeng78@bjmu.edu.cn.
authors: Yi-Hong
authors: YH
authors: Peng
mesh_terms: D000596:Amino Acids; D036022:Capsid Proteins / Q000235:genetics; D015331:Cohort Studies; D004770:Enterovirus / Q000145:classification / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics; D006232:Hand, Foot and Mouth Disease / Q000175:diagnosis / Q000503:physiopathology / Q000821:virology*; D006801:Humans; D010641:Phenotype; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D017423:Sequence Analysis, RNA
chemical_list: D000596:Amino Acids; D036022:Capsid Proteins; D012367:RNA, Viral
keywords: Amino acid site; Clinical phenotype; Enterovirus A71; Pathogenicity; Phylogenetic analysis