title: Structural, antioxidant, and emulsifying activities of fucoidan from Saccharina japonica using pressurized liquid extraction.
abstract: Pressurized liquid extraction (PLE) was utilized to extract sulfated polysaccharides (fucoidan) from brown seaweed Saccharina japonica. Various conditions of temperature (80-200°C), pressure (5-100bar), and solvents (water, 0.1% sodium hydroxide, 0.1% formic acid, 70% ethanol, 50% ethanol, and 25% ethanol) were assessed; the best crude fucoidan (CF) yield was 8.23%, obtained from 140°C and 50bar (sodium hydroxide). Compositional analysis, FT-IR, molecular weight, monosaccharides, TGA, UV-vis, XRD, and elemental analysis confirm that extracted polysaccharides revealed the features of fucoidan. Fucose was the main monosaccharide present in CF obtained by various solvent systems. All CF showed antioxidant activities as measured by DPPH radical and ABTS(+) radical scavenging. CF demonstrates good emulsion-stabilizing capacities, especially with vegetable oils. This study demonstrates that PLE is an efficacious method for enhancing the yield of polysaccharides from S. japonica and that it could be a potential source of natural antioxidants and emulsifiers.
authors: Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Namgu, Busan, 608-737, Republic of Korea.
authors: Periaswamy Sivagnanam
authors: PS
authors: Saravana
authors: Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Namgu, Busan, 608-737, Republic of Korea.
authors: Yeon-Jin
authors: YJ
authors: Cho
authors: Department of Chemical Engineering, Pukyong National University, 365 Sinseon-ro, Namgu, Busan, 608-737, Republic of Korea.
authors: Yong-Beom
authors: YB
authors: Park
authors: Department of Chemical Engineering, Pukyong National University, 365 Sinseon-ro, Namgu, Busan, 608-737, Republic of Korea.
authors: Hee-Chul
authors: HC
authors: Woo
authors: Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Namgu, Busan, 608-737, Republic of Korea. Electronic address: bschun@pknu.ac.kr.
authors: Byung-Soo
authors: BS
authors: Chun
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D045703:Emulsifying Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D004655:Emulsions / Q000737:chemistry; D059625:Liquid-Liquid Extraction / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D011312:Pressure; D012624:Seaweed / Q000737:chemistry*; D012997:Solvents; D013431:Sulfates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013696:Temperature
chemical_list: D000975:Antioxidants; D045703:Emulsifying Agents; D004655:Emulsions; D011134:Polysaccharides; D012997:Solvents; D013431:Sulfates; C007789:fucoidan
keywords: Antioxidant activity; Emulsifying activity; Fucoidan; Pressurized liquid extraction; Saccharina japonica; Sulfated polysaccharides