title: Possible senescence associated change in the predominant a-Na+/K+ ATP-ase isoform in the renal cortex of the rat.
abstract: With aging the kidney exhibits progressive deterioration, with a decrease in renal function. Most of the filtered Na+ is actively reabsorbed in the proximal tubules through different transporters located in apical membrane. This process is possible because basolateral Na+/K+-ATP-ase generates electrochemical conditions necessary for energetically favorable Na+ transport. The a-subunit is the catalytic domain of Na+/K+-ATP-ase. There are three isoforms of the a/subunit present in rat kidney. The present study was undertaken to examine the expression pattern of rat a-Na+/K+-ATP-ase during senescence. We tested the impact of aging on mRNA expression of a-Na+/K+-ATP-ase in cortex and medulla of aged Wistar rats. We observed a significant expression decrease in mRNA levels and a possible change of isoform in the cortex of aged animals. These expression changes observed for a subunit could be contributing to affect the renal function in conditions of water and salt stress.
authors: Centro de Estudios en Salud y Medio Ambiente, Escuela de Ciencia y Tecnología, Universidad Nacional de General San Martín, San Martín, Buenos Aires, Argentina.
authors: María Constanza
authors: MC
authors: Potilinski
authors: Centro de Estudios en Salud y Medio Ambiente, Escuela de Ciencia y Tecnología, Universidad Nacional de General San Martín, San Martín, Buenos Aires, Argentina.
authors: Rosalía
authors: R
authors: Moretta
authors: Centro de Estudios en Salud y Medio Ambiente, Escuela de Ciencia y Tecnología, Universidad Nacional de General San Martín, San Martín, Buenos Aires, Argentina.
authors: Leonardo
authors: L
authors: Casal
authors: Centro de Estudios en Salud y Medio Ambiente, Escuela de Ciencia y Tecnología, Universidad Nacional de General San Martín, San Martín, Buenos Aires, Argentina.
authors: Eduardo
authors: E
authors: García Gras
authors: Centro de Estudios en Salud y Medio Ambiente, Escuela de Ciencia y Tecnología, Universidad Nacional de General San Martín, San Martín, Buenos Aires, Argentina. E-mail: carlos.amorena@unsam.edu.ar.
authors: Carlos E
authors: CE
authors: Amorena
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D007672:Kidney Cortex / Q000201:enzymology*; D007679:Kidney Medulla / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase
keywords: aging; kidney; sodium-potassium-exchanging ATPase