title: CML Mouse Model Generated from Leukemia Stem Cells.
abstract: Chronic myeloid leukemia (CML) is a myeloproliferative disorder with a high number of well-differentiated neutrophils in peripheral blood and myeloid cells in bone marrow (BM). CML is derived from the hematopoietic stem cells (HSCs) with the Philadelphia chromosome (Ph(+), t(9;22)-(q34;q11)), resulting in generating a fusion oncogene, BCR/ABL1. HSCs with Ph(+) are defined as leukemia stem cells (LSCs), a subpopulation cell at the apex of hierarchies in leukemia cells and responsible for the disease continuous propagation. Several kinds of CML models have been developed to reveal the mechanism of CML pathogenesis and evaluate therapeutic drugs in the past three decades. Here, we describe the procedures to generate a CML mouse model by introducing BCR/ABL1 into Lin(-)Sca1(+) cKit(+) population cells purified from mouse bone marrow. In CML retroviral transduction/transplantation mouse models, this modified model can mimic CML pathogenesis on high fidelity.
authors: Department of Hematology, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, No.17, the Third Part Renmin South Road, Chengdu, Sichuan, 610041, China. huyiguo@scu.edu.cn.
authors: Yiguo
authors: Y
authors: Hu
mesh_terms: D000818:Animals; D000067696:Ataxin-1 / Q000235:genetics; D004195:Disease Models, Animal; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000473:pathology*; D051379:Mice; D041681:NIH 3T3 Cells; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000473:pathology; D010677:Philadelphia Chromosome; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: D000067696:Ataxin-1; C000593750:Atxn1 protein, mouse; D019009:Proto-Oncogene Proteins c-kit; D016044:Fusion Proteins, bcr-abl
keywords: BCR/ABL1; Chronic myeloid leukemia (CML); Leukemia stem cells (LSCs); Mouse model; Retroviral transduction/transplantation