title: In Vitro-Pooled shRNA Screening to Identify Determinants of Radiosensitivity.
abstract: Short hairpin RNA (shRNA)-pooled screening is a valuable and cost-effective tool for assaying the contribution of individual genes to cell viability and proliferation on a genomic scale. Here we describe the key considerations for the design and execution of a pooled shRNA screen to identify determinants of radiosensitivity.
authors: Adaptimmune Ltd, 91 Park Drive, Milton Park, Oxon, OX14 4RY, UK. alessandro_ceroni@hotmail.com.
authors: Alessandro
authors: A
authors: Ceroni
authors: Department of Oncology, University of Oxford-Old Road Campus, Oxford, UK.
authors: Geoff S
authors: GS
authors: Higgins
authors: Target Discovery Institute, Nuffield Department of Medicine, University of Oxford-Old Road Campus, Oxford, UK.
authors: Daniel V
authors: DV
authors: Ebner
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002452:Cell Count; D015723:Gene Library; D023281:Genomics / Q000379:methods*; D057809:HEK293 Cells; D006583:Hexadimethrine Bromide / Q000494:pharmacology; D006801:Humans; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000528:radiation effects*; D011836:Radiation Tolerance / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D034741:RNA, Small Interfering; D006583:Hexadimethrine Bromide
keywords: Genomic screening; Lentivirus; Pooled library; Radiosensitivity; shRNA