title: A novel method for dengue virus detection and antibody screening using a graphene-polymer based electrochemical biosensor.
abstract: Dengue fever is a major disease that kills many people in the developing world every year. During early infection, a patient displays a high temperature without other signs. After this stage, and without proper treatment, serious damage to internal organs can happen, which occasionally leads to death. A rapid technique for the early detection of dengue virus (DENV) could reduce the number of fatalities. This study presents a new technique for the detection, classification and antibody screening of DENV based on electrochemical impedance spectroscopy (EIS). We found that the charge transfer resistance (Rct) of a gold electrode coated with graphene oxide reinforced polymer was influenced by virus type and quantity exposed on the surface. Molecular recognition capability established during the GO-polymer composite preparation was used to explain this observation. The linear dependence of Rctversus virus concentrations ranged from 1 to 2×103pfu/mL DENV with a 0.12 pfu/mL detection limit.
authors: Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors: Krongkaew
authors: K
authors: Navakul
authors: Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors: Chompunuch
authors: C
authors: Warakulwit
authors: Siriraj Center of Excellence in Biomedical Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors: Pa-Thai
authors: PT
authors: Yenchitsomanus
authors: Siriraj Center of Excellence in Biomedical Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors: Aussara
authors: A
authors: Panya
authors: Department of Physical Chemistry, Faculty for Chemistry, University of Vienna, Vienna, Austria.
authors: Peter A
authors: PA
authors: Lieberzeit
authors: Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand. Electronic address: fscicsm@ku.ac.th.
authors: Chak
authors: C
authors: Sangma
mesh_terms: D015374:Biosensing Techniques; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus / Q000276:immunology*; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold; D006108:Graphite; D006801:Humans; D011108:Polymers
chemical_list: D011108:Polymers; D006046:Gold; D006108:Graphite
keywords: Biosensor; Dengue; Electrochemical impedance spectroscopy; Graphene; Polymer matrix composite; Virus detection