title: Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy's side-necked turtle) in urban environment.
abstract: Leptospira spp., a zoonotic agent relevant for public health, occurs frequently in tropical regions. The aquatic environment represents a viable survival and transmission pathway. This study aimed to investigate the presence of anti-Leptospira spp. antibodies in Phrynops geoffroanus (Geoffroy's side-necked turtle) serum samples using the microagglutination test (MAT), and Leptospira spp. in gastric and cloacal lavage samples using the polymerase chain reaction (PCR) technique. Antibodies against nine different Leptospira spp. serovars were detected in 45.45% (30/66) of the serum samples. Specific amplification of Leptospira spp. genomic material (331bp) was observed in 16.67% (11/66) of the samples. In conclusion, these freshwater testudines host Leptospira spp. and eliminate them. This situation may represent a risk to public health, especially to people who use urban streams for fishing and recreational activities. Additionally, we described some Leptospira spp. serovars, not yet reported in testudines, detected here in P. geoffroanus.
authors: Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil. Electronic address: julepaulavet@hotmail.com.
authors: J P
authors: JP
authors: Oliveira
authors: Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil. Electronic address: aline.kawanami@gmail.com.
authors: A E
authors: AE
authors: Kawanami
authors: Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil. Electronic address: aslsilva@yahoo.com.br.
authors: A S L
authors: ASL
authors: Silva
authors: Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil. Electronic address: dgchungbr@yahoo.com.
authors: D G
authors: DG
authors: Chung
authors: Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil. Electronic address: werther@fcav.unesp.br.
authors: K
authors: K
authors: Werther
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000469:parasitology; D001938:Brazil; D004199:Disease Vectors; D006801:Humans; D007919:Leptospira / Q000302:isolation & purification*; D007922:Leptospirosis / Q000635:transmission*; D016133:Polymerase Chain Reaction; D065288:Serogroup; D014426:Turtles / Q000469:parasitology*; D014504:Urban Health
keywords: Leptospira; Public health; Reptile; Terrapin; Zoonosis