title: Neuroprotective Effect of Matrine in Mouse Model of Vincristine-Induced Neuropathic Pain.
abstract: Chemotherapy drugs such as vincristine (VCR) can cause neuropathic pain, and there is still lack of ideal strategy to treat it. The current study was designed to investigate effect of matrine (MT) on VCR-induced neuropathic pain in animal model. VCR (75 μg/kg, i.p. for 10 consecutive days) was administered to induce painful neuropathy model in mice. MT (15, 30 and 60 mg/kg, i.p.) and pregabalin (10 mg/kg, i.p.) were administered for 11 consecutive days. Various tests were performed to assess the degree of pain at different days (1, 6, 11, 16, and 21). Von Frey hair, hot plate, cold-plate and paw pressure tests were conducted to assess the degree of mechanical allodynia, thermal hyperalgesia, cold allodynia and mechanical hyperalgesia in the hind paw respectively. The electrophysiological and histopathological changes were also analyzed. Furthermore, tissue malondialdehyde (MDA), total antioxidant capacity (T-AOC),superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total calcium (TCA), myeloperoxidase (MPO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10) were measured to investigate possible involvement of MT in inflammation and oxidative stress. Administration of MT attenuated the VCR-induced behavioral alterations as well as electrophysiological and histopathological changes in a dose dependent manner. Further, MT also attenuated the VCR-induced oxidative stress (MDA, T-AOC, GSH-Px, SOD and TCA) and inflammation (MPO, TNF-α, IL-6 and IL-10). Taken together, MT ameliorated VCR-induced painful neuropathy, which might be attributed to neuroprotective effects by subsequent reduction in oxidative stress and anti-inflammatory actions.
authors: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, 1160 Shengli Street, Xingqing, Ningxia Hui Autonomous Region, Yinchuan, 750004, China.
authors: Shuai-Shuai
authors: SS
authors: Gong
authors: College of Nursing, Ningxia Medical University, Yinchuan, 750004, China.
authors: Yu-Xiang
authors: YX
authors: Li
authors: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, 1160 Shengli Street, Xingqing, Ningxia Hui Autonomous Region, Yinchuan, 750004, China.
authors: Meng-Ting
authors: MT
authors: Zhang
authors: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, 1160 Shengli Street, Xingqing, Ningxia Hui Autonomous Region, Yinchuan, 750004, China.
authors: Juan
authors: J
authors: Du
authors: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, 1160 Shengli Street, Xingqing, Ningxia Hui Autonomous Region, Yinchuan, 750004, China.
authors: Peng-Sheng
authors: PS
authors: Ma
authors: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, 1160 Shengli Street, Xingqing, Ningxia Hui Autonomous Region, Yinchuan, 750004, China.
authors: Wan-Xia
authors: WX
authors: Yao
authors: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, 1160 Shengli Street, Xingqing, Ningxia Hui Autonomous Region, Yinchuan, 750004, China.
authors: Ru
authors: R
authors: Zhou
authors: Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, 750004, China.
authors: Yang
authors: Y
authors: Niu
authors: Ningxia Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan, 750004, China.
authors: Tao
authors: T
authors: Sun
authors: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, 1160 Shengli Street, Xingqing, Ningxia Hui Autonomous Region, Yinchuan, 750004, China. yujq910315@163.com.|Ningxia Hui Medicine Modern Engineering Research Center and Collaborative Innovation Center, Ningxia Medical University, Yinchuan, 750004, China. yujq910315@163.com.
authors: Jian-Qiang
authors: JQ
authors: Yu
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004195:Disease Models, Animal; D051379:Mice; D009437:Neuralgia / Q000139:chemically induced / Q000188:drug therapy*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D011807:Quinolizines / Q000494:pharmacology*; D012584:Sciatic Nerve / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014750:Vincristine / Q000494:pharmacology*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000975:Antioxidants; D018696:Neuroprotective Agents; D011807:Quinolizines; D014409:Tumor Necrosis Factor-alpha; D014750:Vincristine; D000093842:Matrines
keywords: Anti-inflammatory; Electrophysiology; Matrine; Neuropathic pain; Neuroprotective; Vincristine