title: A novel [Mn2(μ-(C6H5)2CHCOO)2(bipy)4](bipy)(ClO4)2complex loaded solid lipid nanoparticles: synthesis, characterization and in vitro cytotoxicity on MCF-7 breast cancer cells.
abstract: Manganese (Mn)-based complexes have been drawing attention due to the fact that they are more effective than other metal complexes. However, the use of Mn(II)-based complexes in medicine remains limited because of certain side effects. The aim of this study was to investigate the cytotoxic and apoptotic effects of a novel Mn(II) complex [Mn2(μ-(C6H5)2CHCOO)2(bipy)4](bipy)(ClO4)2and Mn(II) complex loaded solid lipid nanoparticles (SLNs) on MCF-7 and HUVEC control cells. The average diameter of Mn(II) complex was about 1120 ± 2.43 nm, while the average particle size of Mn(II) complex-SLNs was ∼340 ± 2.27 nm. The cytotoxic effects of Mn(II) complex and Mn(II)-SLNs were 86.8 and 66.4%, respectively (p < .05). Additionally, both Mn(II) complex (39.25%) and Mn(II)-SLNs (38.05%) induced apoptosis and increased the arrest of G0/G1phase. However, Mn(II) complex exerted toxic effects on the HUVEC control cell (63.4%), whereas no toxic effects was observed when treated with Mn(II)-SLNs at 150 μM. As a consequence, SLNs might be potentially used for metal-based complexes in the treatment of cancer due to reducing size and toxic effects of metal-based complexes.
authors: a Deparment of Medical Biology, Faculty of Medicine , Uludag University , Bursa , Turkey.
authors: G
authors: G
authors: Guney Eskiler
authors: a Deparment of Medical Biology, Faculty of Medicine , Uludag University , Bursa , Turkey.
authors: G
authors: G
authors: Cecener
authors: b Central Research Laboratory Research and Application Center , Eskisehir Osmangazi University , Eskisehir , Turkey.
authors: G
authors: G
authors: Dikmen
authors: c Deparment of Chemistry, Faculty of Science , Anadolu University , Eskisehir , Turkey.
authors: I
authors: I
authors: Kani
authors: a Deparment of Medical Biology, Faculty of Medicine , Uludag University , Bursa , Turkey.
authors: U
authors: U
authors: Egeli
authors: a Deparment of Medical Biology, Faculty of Medicine , Uludag University , Bursa , Turkey.
authors: B
authors: B
authors: Tunca
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D003603:Cytotoxins / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D061986:MCF-7 Cells; D008345:Manganese / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size
chemical_list: D000970:Antineoplastic Agents; D003603:Cytotoxins; D008055:Lipids; D008345:Manganese
keywords: 2,2´-bipyridine; Metal-based ligands; Mn(II) complex; cancer; drug delivery systems; solid lipid nanoparticles (SLNs)