title: Increased oxygen exposure alters collagen expression and tissue architecture during ligature-induced periodontitis.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effects of increased oxygen availability on gene expression and on collagen deposition/maturation in the periodontium following disease.
  
  
  METHODS
  Male Wistar rats had ligatures placed around their molars to induce periodontal disease, and a subset of animals underwent hyperbaric oxygen (HBO) treatment for 2 h twice per day. At 15 and 28 d, tissue gene expression of COL1A1, transforming growth factor-β1 and alkaline phosphatase was determined; other histological samples were stained with Picrosirius red to evaluate levels of collagen deposition, maturation and thickness.
  
  
  RESULTS
  In animals that underwent HBO treatment, type I collagen expression was higher and collagen deposition, maturation and thickness were more robust. Reduced mRNA levels of transforming growth factor-beta1 and alkaline phosphatase in HBO-treated rats on day 28 suggested that a quicker resolution in both soft tissue and bone remodeling occurred following oxygen treatment. No differences in inflammation were observed between groups.
  
  
  CONCLUSIONS
  The extracellular matrix regenerated more quickly in the HBO-treated group as evidenced by higher collagen expression, deposition and maturation.
authors: Department of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.|Center for Wound Healing and Tissue Regeneration, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
authors: P K
authors: PK
authors: Gajendrareddy
authors: Faculty of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.|Faculty of Dentistry, Department of Oral Biology, University of Oslo, Oslo, Norway.
authors: R
authors: R
authors: Junges
authors: Department of Oral and Maxillofacial Surgery, West Virginia University, Morgantown, WV, USA.
authors: G
authors: G
authors: Cygan
authors: Department of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.|Center for Wound Healing and Tissue Regeneration, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.
authors: Y
authors: Y
authors: Zhao
authors: Center for Wound Healing and Tissue Regeneration, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.|School of Dentistry, Oregon Health and Science University, Portland, OR, USA.
authors: P T
authors: PT
authors: Marucha
authors: Center for Wound Healing and Tissue Regeneration, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.|Department of Biobehavioral Health, The Pennsylvania State University, University Park, PA, USA.|College of Nursing, The Pennsylvania State University, University Park, PA, USA.
authors: C G
authors: CG
authors: Engeland
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D003094:Collagen / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D015870:Gene Expression / Q000187:drug effects; D006931:Hyperbaric Oxygenation; D008297:Male; D010518:Periodontitis / Q000378:metabolism* / Q000473:pathology; D010519:Periodontium / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D053773:Transforming Growth Factor beta1; D003094:Collagen; D000469:Alkaline Phosphatase
keywords: alkaline phosphatase; collagen type I; cytokine; hyperbaric oxygen; inflammation; transforming growth factor beta1