title: Calcium-Sensing Receptor in Human Peripheral Blood T Lymphocytes Is Involved in the AMI Onset and Progression through the NF-κB Signaling Pathway.
abstract: Acute myocardial infarction (AMI) is a condition triggered by an inflammatory process that seriously affects human health. Calcium-sensing receptor (CaSR) in T lymphocytes is involved during the inflammation reaction. However, the relationship between them is not very clear. In this study, we collected human peripheral blood T lymphocytes from patients with AMI and in different stages of percutaneous coronary intervention (PCI) (at the onset of AMI, the first day after PCI (PCI-1), PCI-3, and PCI-5) to study the CaSR and NF-κB pathway protein expression, cytokine release and T cell apoptosis. The results showed that the expressions of CaSR, P-p65, Caspase-12, and the secretions of Th-1 and Th-2 type cytokines were increased at the onset of AMI, especially on the PCI-1. Meanwhile, the apoptosis rate of CD(3+), CD(4+) and CD(8+) T lymphocytes also increased. However, from PCI-3, all the indicators began to decline. In addition, we also found that positive CaSR small interfering RNA (siRNA) transfection in T lymphocytes and NF-κB pathway blocker Bay-11-7082 reversed the increased expressions of CaSR, P-p65, Caspase-12, reduced the secretions of Th-1 and Th-2 type cytokines, and decreased T lymphocytes apoptosis rate not only in the AMI patients but also in the normal controls. All of these results indicated that CaSR in the human peripheral blood T lymphocytes were involved in the AMI onset and progression, which probably was related to the NF-κB pathway. Our study demonstrated the relationship between AMI and CaSR, and will provide new effective prevention theory and new targets for drug treatment.
authors: Department of Clinical Laboratory, Harbin Medical University Cancer Hospital, Harbin 150086, China. zengjingya121@163.com.
authors: Jing-Ya
authors: JY
authors: Zeng
authors: Blood Transfusion Department, the First Affiliated Hospital of Harbin Medical University, Harbin 150086, China. 18745793906@126.com.
authors: Jing-Jing
authors: JJ
authors: Du
authors: Department of Clinical Laboratory, Harbin Medical University Cancer Hospital, Harbin 150086, China. 13019019570@163.com.
authors: Ying
authors: Y
authors: Pan
authors: Department of Cardiology, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China. wujian780805@163.com.
authors: Jian
authors: J
authors: Wu
authors: Department of Clinical Laboratory, Harbin Medical University Cancer Hospital, Harbin 150086, China. 18346144845@163.com.
authors: Hai-Liang
authors: HL
authors: Bi
authors: Department of Clinical Laboratory, Harbin Medical University Cancer Hospital, Harbin 150086, China. cuibaohongsun@163.com.
authors: Bao-Hong
authors: BH
authors: Cui
authors: Department of Clinical Laboratory, Harbin Medical University Cancer Hospital, Harbin 150086, China. zhaitaiyu0810@163.com.
authors: Tai-Yu
authors: TY
authors: Zhai
authors: Department of Cardiology, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China. ssunyyong@126.com.
authors: Yong
authors: Y
authors: Sun
authors: Department of Clinical Laboratory, Harbin Medical University Cancer Hospital, Harbin 150086, China. syh200415@126.com.
authors: Yi-Hua
authors: YH
authors: Sun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D053454:Caspase 12 / Q000378:metabolism; D005260:Female; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology*; D016328:NF-kappa B / Q000378:metabolism*; D011446:Prospective Studies; D044169:Receptors, Calcium-Sensing / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D044169:Receptors, Calcium-Sensing; D053454:Caspase 12
keywords: acute myocardial infarction; calcium-sensing receptor; cytokine; lymphocyte; signaling pathway