title: Reduction-responsive multifunctional hyperbranched polyaminoglycosides with excellent antibacterial activity, biocompatibility and gene transfection capability.
abstract: There is an increasing demand in developing of multifunctional materials with good antibacterial activity, biocompatibility and drug/gene delivery capability for next-generation biomedical applications. To achieve this purpose, in this work series of hydroxyl-rich hyperbranched polyaminoglycosides of gentamicin, tobramycin, and neomycin (HP and SS-HP with redox-responsive disulfide bonds) were readily synthesized via ring-opening reactions in a one-pot manner. Both HP and SS-HP exhibit high antibacterial activity toward Escherichia coli and Staphylococcus aureus. Meanwhile, the hemolysis assay of the above materials shows good biocompatibility. Moreover, SS-HPs show excellent gene transfection efficiency in vitro due to the breakdown of reduction-responsive disulfide bonds. For an in vivo anti-tumor assay, the SS-HP/p53 complexes exhibit potent inhibition capability to the growth of tumors. This study provides a promising approach for the design of next-generation multifunctional biomedical materials.
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China; Key Laboratory of Carbon Fiber and Functional Polymers, Beijing University of Chemical Technology, Ministry of Education, Beijing 100029, China; Beijing Laboratory of Biomedical Materials, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors: Yajun
authors: Y
authors: Huang
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China; Key Laboratory of Carbon Fiber and Functional Polymers, Beijing University of Chemical Technology, Ministry of Education, Beijing 100029, China; Beijing Laboratory of Biomedical Materials, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors: Xiaokang
authors: X
authors: Ding
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China; Key Laboratory of Carbon Fiber and Functional Polymers, Beijing University of Chemical Technology, Ministry of Education, Beijing 100029, China; Beijing Laboratory of Biomedical Materials, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors: Yu
authors: Y
authors: Qi
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China; Key Laboratory of Carbon Fiber and Functional Polymers, Beijing University of Chemical Technology, Ministry of Education, Beijing 100029, China; Beijing Laboratory of Biomedical Materials, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: yubr@mail.buct.edu.cn.
authors: Bingran
authors: B
authors: Yu
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China; Key Laboratory of Carbon Fiber and Functional Polymers, Beijing University of Chemical Technology, Ministry of Education, Beijing 100029, China; Beijing Laboratory of Biomedical Materials, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: xufj@mail.buct.edu.cn.
authors: Fu-Jian
authors: FJ
authors: Xu
mesh_terms: D000617:Aminoglycosides / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D010084:Oxidation-Reduction; D010957:Plasmids / Q000008:administration & dosage*; D014162:Transfection / Q000379:methods*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D053769:Nanocapsules
keywords: Aminoglycoside; Antibacterial; Gene vectors; Hyperbranched polymer; Reduction-responsive