title: Thiol-ene click synthesis of L-Cysteine-bonded zwitterionic hydrophilic magnetic nanoparticles for selective and efficient enrichment of glycopeptides.
abstract: Efficient and specific enrichment of low-abundant glycopeptides from complex biological samples is essential to glycoproteomics analysis. Herein, novel magnetic zwitterionic hydrophilic nanoparticles based on thiol-ene click chemistry were synthesized. The functionalized magnetic nanoparticles exhibited superior performance in glycopeptide enrichment of HRP tryptic digest, demonstrating low detection limit (0.04ng/μL), high selectivity (a mixture of HRP and BSA at the mass ratio of 1:50) and reproducibility (5 repeating cycles). In addition, the material was successfully applied to glycopeptide enrichment from human serum. The outstanding results indicate the potential of the method in the development of glycoproteomics analysis in real biological samples.
authors: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, P.R. China.
authors: Runqing
authors: R
authors: Wu
authors: Department of Orthopaedic Surgery, Second Military Medical University, The Affiliated Changhai Hospital of Shanghai 200433, P.R. China. Electronic address: xysmmu@163.com.
authors: Yang
authors: Y
authors: Xie
authors: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, P.R. China. Electronic address: chdeng@fudan.edu.cn.
authors: Chunhui
authors: C
authors: Deng
mesh_terms: D057930:Click Chemistry; D003545:Cysteine / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry
chemical_list: D006020:Glycopeptides; D058185:Magnetite Nanoparticles; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D006735:Horseradish Peroxidase; D014357:Trypsin; D003545:Cysteine
keywords: Enrichment; Glycopeptide; Hydrophilicity; MALDI-TOF-MS; Thiol-ene click chemistry