title: Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution.
abstract: Membrane protein transport on the single protein level still evades detailed analysis, if the substrate translocated is non-electrogenic. Considerable efforts have been made in this field, but techniques enabling automated high-throughput transport analysis in combination with solvent-free lipid bilayer techniques required for the analysis of membrane transporters are rare. This class of transporters however is crucial in cell homeostasis and therefore a key target in drug development and methodologies to gain new insights desperately needed. The here presented manuscript describes the establishment and handling of a novel biochip for the analysis of membrane protein mediated transport processes at single transporter resolution. The biochip is composed of microcavities enclosed by nanopores that is highly parallel in its design and can be produced in industrial grade and quantity. Protein-harboring liposomes can directly be applied to the chip surface forming self-assembled pore-spanning lipid bilayers using SSM-techniques (solid supported lipid membranes). Pore-spanning parts of the membrane are freestanding, providing the interface for substrate translocation into or out of the cavity space, which can be followed by multi-spectral fluorescent readout in real-time. The establishment of standard operating procedures (SOPs) allows the straightforward establishment of protein-harboring lipid bilayers on the chip surface of virtually every membrane protein that can be reconstituted functionally. The sole prerequisite is the establishment of a fluorescent read-out system for non-electrogenic transport substrates. High-content screening applications are accomplishable by the use of automated inverted fluorescent microscopes recording multiple chips in parallel. Large data sets can be analyzed using the freely available custom-designed analysis software. Three-color multi spectral fluorescent read-out furthermore allows for unbiased data discrimination into different event classes, eliminating false positive results. The chip technology is currently based on SiO2 surfaces, but further functionalization using gold-coated chip surfaces is also possible.
authors: Institute of Biochemistry, Biocenter, Goethe University Frankfurt.
authors: Michael
authors: M
authors: Urban
authors: Nanospot GmbH.
authors: Marc
authors: M
authors: Vor der Brüggen
authors: Institute of Biochemistry, Biocenter, Goethe University Frankfurt; tampe@em.uni-frankfurt.de.
authors: Robert
authors: R
authors: Tampé
mesh_terms: D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism; D008081:Liposomes; D058608:Nanopores; D012822:Silicon Dioxide
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D012822:Silicon Dioxide