title: T cell abundance in blood predicts acute organ toxicity in chemoradiotherapy for head and neck cancer.
abstract: Treatment of head and neck squamous cell carcinoma (HNSCC) by chemoradiotherapy (CRT) often results in high-grade acute organ toxicity (HGAOT). As these adverse effects impair the patients' quality of life and the feasibility of the planned therapy, we sought to analyze immunological parameters in tumor material and blood samples obtained from 48 HNSCC patients in order to assess the potential to predict the individual acute organ toxicity. T cells in the tumor stroma were enriched in patients developing HGAOT whereas levels of soluble factors in the plasma and gene expression in whole blood did not coincide with the occurrence of acute organ toxicity. In contrast, the frequency and absolute numbers of selected leukocyte subpopulations measured in samples of peripheral blood mononuclear cells (PBMCs) directly before the beginning of CRT were significantly different in patients with HGAOT as compared to those without. When we validated several potential markers including the abundance of T cells in a small prospective study with 16 HNSCC patients, we were able to correctly predict acute organ toxicity in up to 81% of the patients. We conclude that analysis of PBMCs by fluorescence-activated cell sorting (FACS) might be a convenient strategy to identify patients at risk of developing HGAOT caused by CRT, which might allow to adapt the treatment regimen and possibly improve disease outcome.
authors: Institute for Cellular and Molecular Immunology, University Medical Center, Georg-August-University Göttingen, Germany.
authors: L Milena
authors: LM
authors: Beschel
authors: Department of Radiotherapy and Radiooncology, University Medical Center, Georg-August-University Göttingen, Germany.
authors: Martin
authors: M
authors: Leu
authors: Institute for Cellular and Molecular Immunology, University Medical Center, Georg-August-University Göttingen, Germany.
authors: Sybille D
authors: SD
authors: Reichardt
authors: Department of Radiotherapy and Radiooncology, University Medical Center, Georg-August-University Göttingen, Germany.
authors: Margret
authors: M
authors: Rave-Fränk
authors: Department of Radiotherapy and Radiooncology, University Medical Center, Georg-August-University Göttingen, Germany.|Institute of Clinical Pharmacology, University Medical Center, Georg-August-University Göttingen, Germany.
authors: Markus A
authors: MA
authors: Schirmer
authors: Institute of Neuropathology, University Medical Center, Georg-August-University Göttingen, Germany.
authors: Christine
authors: C
authors: Stadelmann
authors: Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center, Georg-August-University Göttingen, Germany.
authors: Martin
authors: M
authors: Canis
authors: University Medical Center, Georg-August-University Göttingen, Germany.|Present address: Strahlentherapie Radiologie München, Germany.
authors: Hendrik A
authors: HA
authors: Wolff
authors: Institute for Cellular and Molecular Immunology, University Medical Center, Georg-August-University Göttingen, Germany.
authors: Holger M
authors: HM
authors: Reichardt
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000276:immunology* / Q000628:therapy; D049109:Cell Proliferation; D059248:Chemoradiotherapy / Q000009:adverse effects*; D005260:Female; D005500:Follow-Up Studies; D006258:Head and Neck Neoplasms / Q000097:blood / Q000276:immunology* / Q000628:therapy; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011379:Prognosis; D012189:Retrospective Studies; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D018675:Toxicity Tests; D014407:Tumor Cells, Cultured
keywords: T cell; acute organ toxicity; chemoradiotherapy; head and neck squamous cell carcinoma; predictive marker