title: Genipin Cross-Linked Glucose Oxidase and Catalase Multi-enzyme for Gluconic Acid Synthesis.
abstract: In this work, glucose oxidase (GOD) and catalase (CAT) were used simultaneously to produce gluconic acid from glucose. In order to reduce the distance between the two enzymes, and therefore improve efficiency, GOD and CAT were cross-linked together using genipin. Improvements in gluconic acid production were due to quick removal of harmful intermediate hydrogen peroxide by CAT. GOD activity was significantly affected by the proportion of CAT in the system, with GOD activity in the cross-linked multi-enzyme (CLME) being 10 times higher than that in an un-cross-linked GOD/CAT mixture. The glucose conversion rate after 15 h using 15 % glucose was also 10 % higher using the CLME than was measured using a GOD/CAT mixture.
authors: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
authors: Caixia
authors: C
authors: Cui
authors: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
authors: Haibin
authors: H
authors: Chen
authors: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China. chenbq@mail.buct.edu.cn.
authors: Biqiang
authors: B
authors: Chen
authors: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
authors: Tianwei
authors: T
authors: Tan
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D002374:Catalase / Q000737:chemistry*; D003432:Cross-Linking Reagents; D004789:Enzyme Activation; D005942:Gluconates / Q000138:chemical synthesis*; D005947:Glucose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D039823:Iridoids / Q000737:chemistry*; D009097:Multienzyme Complexes / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D005942:Gluconates; D039823:Iridoids; D009097:Multienzyme Complexes; C007834:genipin; D005949:Glucose Oxidase; D002374:Catalase; D005947:Glucose; C030691:gluconic acid
keywords: Catalase; Cross-linking; Genipin; Gluconic acid; Glucose oxidase