title: Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals.
abstract: In this paper, a microthermograph, temperature mapping with high spatial resolution, was established using luminescent molecules embedded ultrathin polymeric films (nanosheets), and demonstrated in a living small animal to map out and visualize temperature shift due to animal's muscular activity. Herein, we report super flexible and self-adhesive (no need of glue) nanothermosensor consisting of stacked two different polymeric nanosheets with thermosensitive (Eu-tris (dinaphthoylmethane)-bis-trioctylphosphine oxide: EuDT) and insensitive (Rhodamine 800) dyes being embedded. Such stacked nanosheets allow for the ratiometric thermometry, with which the undesired luminescence intensity shift due to focal drift or animal's z-axis displacement is eliminated and the desired intensity shift solely due to the temperature shift of the sample (living muscle) can be acquired. With the stacked luminescent nanosheets, we achieved the first-ever demonstration of video filming of chronologically changing temperature-shift distribution from the rest state to the active state of the muscles in the living animal. The polymer nanosheet engineering and in vivo microthermography presented in the paper are promising technologies to microscopically explore the heat production and heat transfer in living cells, tissues, and organisms with high spatial resolution beyond what existing thermometric technologies such as infrared thermography have ever achieved.
authors: Takuya
authors: T
authors: Miyagawa
authors: PRESTO, Japan Science and Technology Agency , 4-1-8 Honcho, Kawaguchi, Saitama 332-0012 Japan.
authors: Toshinori
authors: T
authors: Fujie
authors: Division of Engineering Mechanics, School of Mechanical & Aerospace Engineering, College of Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798 Singapore.
authors: Ferdinandus
authors: Division of Engineering Mechanics, School of Mechanical & Aerospace Engineering, College of Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798 Singapore.
authors: Tat Thang
authors: TT
authors: Vo Doan
authors: Division of Engineering Mechanics, School of Mechanical & Aerospace Engineering, College of Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798 Singapore.
authors: Hirotaka
authors: H
authors: Sato
authors: Shinji
authors: S
authors: Takeoka
mesh_terms: D000269:Adhesives; D000818:Animals; D049449:Luminescence; D013817:Thermography; D013821:Thermometers
chemical_list: D000269:Adhesives
keywords: heat production; luminescent; optical sensor; thermometry; ultrathin film (nanosheet)