title: Construction of genetic parts from the Corynebacterium glutamicum genome with high expression activities.
abstract: OBJECTIVE
  To construct effective genetic expression parts controlling transcription and translation initiation for synthetic biology and heterologous expression in Corynebacterium glutamicum.
  
  
  RESULTS
  Twelve highly expressed genes were identified from the proteomic data of C. glutamicum. Their related sequences were used to construct bicistronic genetic expression parts. Each part contain promoter, 5'-UTR, N-terminal sequence of the source gene and a conserved SD sequence, associated with target gene, forming the bicistronic expression cassette. The enhanced green fluorescent protein (EGFP) expression levels controlled by these novel parts have 1.4 to 790-fold increase in C. glutamicum compared with corresponding promoter-5'-UTR part. One of the bicistronic parts is 1.35 times the EGFP expression of the constitutive-expression pXMJ19. These bicistronic parts had expression advantage compared with conventional promoter-5'-UTR parts.
  
  
  CONCLUSIONS
  Various genetic parts for efficient gene expression can be quickly obtained via this new method.
authors: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
authors: Zihao
authors: Z
authors: Zhao
authors: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China. liuxiuxia@jiangnan.edu.cn.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China. liuxiuxia@jiangnan.edu.cn.
authors: Xiuxia
authors: X
authors: Liu
authors: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
authors: Wei
authors: W
authors: Zhang
authors: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
authors: Yankun
authors: Y
authors: Yang
authors: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
authors: Xiaofeng
authors: X
authors: Dai
authors: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China. baizhonghu@jiangnan.edu.cn.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China. baizhonghu@jiangnan.edu.cn.
authors: Zhonghu
authors: Z
authors: Bai
mesh_terms: D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism; D016680:Genome, Bacterial / Q000235:genetics*; D040901:Proteomics / Q000379:methods
keywords: Corynebacterium glutamicum; Gene expression; Genetic part; Proteomics; Translation coupling