title: Bacterial communities and enzymatic activities in the vegetation-activated sludge process (V-ASP) and related advantages by comparison with conventional constructed wetland.
abstract: A new-developed vegetation-activated sludge process (V-ASP) was implemented for decentralized domestic wastewater treatment, and studied in lab-scale and full-scale. The main purpose of this work was the investigation of biomass activities and microbial communities in V-ASP by comparison with conventional constructed wetland (CW), to unveil the causations of its consistently higher pollutants removal efficiencies. Compared with CWs, V-ASP has greater vegetation nitrogen and phosphorus uptake rates, higher biomass and enzymatic activities, and more bacteria community diversity. The microbial community structure was comprehensively analyzed by using high-throughput sequencing. It was observed that Proteobacteria was dominated in both CWs and V-ASPs, while their subdivisions distribution was rather different. V-ASPs contained a higher nitrite-oxidizing bacteria (Nitrospira) abundances that resulted in a consistently better nitrogen removal efficiency. Hence, a long-term experiment of full-scale V-ASP displayed stably excellent capability in resistance of influent loading shocks and seasonal temperature effect.
authors: Harbin Institute of Technology Shenzhen Graduate School, Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, Shenzhen 518055, China.
authors: Jiajia
authors: J
authors: Yuan
authors: Harbin Institute of Technology Shenzhen Graduate School, Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, Shenzhen 518055, China.
authors: Wenyi
authors: W
authors: Dong
authors: Harbin Institute of Technology Shenzhen Graduate School, Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, Shenzhen 518055, China. Electronic address: sunfeiyun1982@gmail.com.
authors: Feiyun
authors: F
authors: Sun
authors: Harbin Institute of Technology Shenzhen Graduate School, Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, Shenzhen 518055, China.
authors: Ke
authors: K
authors: Zhao
authors: Harbin Institute of Technology Shenzhen Graduate School, Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, Shenzhen 518055, China.
authors: Changhang
authors: C
authors: Du
authors: Harbin Institute of Technology Shenzhen Graduate School, Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, Shenzhen 518055, China.
authors: Yunxian
authors: Y
authors: Shao
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D018533:Biomass; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction; D010758:Phosphorus; D020560:Proteobacteria / Q000145:classification / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D018505:Waste Management / Q000379:methods*; D062065:Wastewater / Q000737:chemistry*; D053833:Wetlands
chemical_list: D009584:Nitrogen; D010758:Phosphorus; D012722:Sewage; D062065:Wastewater
keywords: Bacterial communities; Constructed wetland; Decentralized wastewater treatment; High-throughput sequencing; Vegetation-activated sludge process (V-ASP)