title: Visualizing Herpesvirus Procapsids in Living Cells.
abstract: A complete understanding of herpesvirus morphogenesis requires studies of capsid assembly dynamics in living cells. Although fluorescent tags fused to the VP26 and pUL25 capsid proteins are available, neither of these components is present on the initial capsid assembly, the procapsid. To make procapsids accessible to live-cell imaging, we made a series of recombinant pseudorabies viruses that encoded green fluorescent protein (GFP) fused in frame to the internal capsid scaffold and maturation protease. One recombinant, a GFP-VP24 fusion, maintained wild-type propagation kinetics in vitro and approximated wild-type virulence in vivo The fusion also proved to be well tolerated in herpes simplex virus. Viruses encoding GFP-VP24, along with a traditional capsid reporter fusion (pUL25/mCherry), demonstrated that GFP-VP24 was a reliable capsid marker and revealed that the protein remained capsid associated following entry into cells and upon nuclear docking. These dual-fluorescent viruses made possible the discrimination of procapsids during infection and monitoring of capsid shell maturation kinetics. The results demonstrate the feasibility of imaging herpesvirus procapsids and their morphogenesis in living cells and indicate that the encapsidation machinery does not substantially help coordinate capsid shell maturation.
  
  
  OBJECTIVE
  The family Herpesviridae consists of human and veterinary pathogens that cause a wide range of diseases in their respective hosts. These viruses share structurally related icosahedral capsids that encase the double-stranded DNA (dsDNA) viral genome. The dynamics of capsid assembly and maturation have been inaccessible to examination in living cells. This study has overcome this technical hurdle and provides new insights into this fundamental stage of herpesvirus infection.
authors: Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois, USA.
authors: Oana
authors: O
authors: Maier
authors: School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
authors: Patricia J
authors: PJ
authors: Sollars
authors: School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
authors: Gary E
authors: GE
authors: Pickard
authors: Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois, USA g-smith3@northwestern.edu.
authors: Gregory A
authors: GA
authors: Smith
mesh_terms: D000818:Animals; D002213:Capsid / Q000378:metabolism*; D036022:Capsid Proteins / Q000378:metabolism*; D002460:Cell Line; D002522:Chlorocebus aethiops; D016679:Genome, Viral / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006561:Herpes Simplex / Q000378:metabolism* / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000378:metabolism*; D011558:Herpesvirus 1, Suid / Q000378:metabolism; D008297:Male; D051379:Mice; D014709:Vero Cells; D014764:Viral Proteins / Q000378:metabolism; D019065:Virus Assembly / Q000502:physiology; D053586:Virus Internalization
chemical_list: D036022:Capsid Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins