title: Application of multiplex immunoassay technology to investigations of ocular disease.
abstract: Eye-derived fluids, including tears, aqueous humour and vitreous humour often contain molecular signatures of ocular disease states. These signatures can be composed of cytokines, chemokines, growth factors, proteases and soluble receptors. However, the small quantities (<10 µl) of these fluids severely limit the detection of these proteins by traditional enzyme-linked immunosorbent assay or Western blot. To maximise the amount of information generated from the analysis of these specimens, many researchers have employed multiplex immunoassay technologies for profiling the expression or modification of multiple proteins from minute sample volumes.
authors: Raybiotech, Inc.,Norcross, GA,USA.
authors: Valerie Sloane
authors: VS
authors: Jones
authors: The Affiliated third Hospital of Sun Yat-sen University, Sun Yat-sen University,Guangdong,China.
authors: Jian
authors: J
authors: Wu
authors: RayBiotech, Inc.,Guangzhou,China.
authors: Si-Wei
authors: SW
authors: Zhu
authors: Raybiotech, Inc.,Norcross, GA,USA.
authors: Ruo-Pan
authors: RP
authors: Huang
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000276:immunology / Q000378:metabolism; D015415:Biomarkers; D005128:Eye Diseases / Q000175:diagnosis* / Q000209:etiology / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D006801:Humans; D007118:Immunoassay / Q000379:methods; D020543:Proteome; D040901:Proteomics / Q000379:methods; D013666:Tears / Q000276:immunology / Q000378:metabolism
chemical_list: D015415:Biomarkers; D020543:Proteome