title: Photosensitizer effect of 9-hydroxypheophorbide α on diode laser-irradiated laryngeal cancer cells: Oxidative stress-directed cell death and migration suppression.
abstract: The present study aimed to investigate the effect, and elucidate the potential mechanisms, of 9-hydroxypheophorbide α-based photodynamic therapy (9-HPbD-PDT) on apoptosis and necrosis induction, and migration suppression of laryngeal cancer AMC-HN-3 (HN-3) cells. Phototoxicity initiated by 9-HPbD-PDT on HN-3 cells was observed in a photosensitizer dose-dependent pattern. There was an initial increase of apoptotic cells coupled with gradual enhancement of reactive oxygen series (ROS) generation at lower doses of 9-HPbD. By contrast, at a higher dose of 9-HPbD, there was a clear increase of necrotic cells with a gradual decrease of ROS generation. Following PDT, an elevated percentage of apoptotic cells with shrinkage or condensing nuclei was observed using Hoechst 33342/propidium iodide double staining, and an upregulated expression of poly ADP-ribose polymerase was detected through western blotting. A disruption of the mitochondrial membrane potential was detected 2 h following PDT. Significant suppression of cell migration and downregulation of epidermal growth factor receptor (EGFR) expression were recorded following PDT. These results indicate that the distribution of photosensitizer leads to differences in the generation of ROS, which subsequently determines the type of cell death. Overall, mitochondrial activation under oxidative stress is important in the 9-HPbD-PDT-induced apoptosis of HN-3 cells. Migration suppression of HN-3 cells following PDT may be associated with the inhibited expression of EGFR, due to oxidative stress.
authors: Department of Otolaryngology-Head and Neck Surgery, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, Shanghai 200031, P.R. China; Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, P.R. China.
authors: Peijie
authors: P
authors: He
authors: Institute of Radiation Medicine, Fudan University, Shanghai 200032, P.R. China.
authors: Shen
authors: S
authors: Bo
authors: Department of Otolaryngology-Head and Neck Surgery, Medical Laser Research Center, College of Medicine, Dankook University, Cheonan 330-715, Republic of Korea.
authors: Phil-Sang
authors: PS
authors: Chung
authors: Department of Otolaryngology-Head and Neck Surgery, Medical Laser Research Center, College of Medicine, Dankook University, Cheonan 330-715, Republic of Korea.
authors: Jin-Chul
authors: JC
authors: Ahn
authors: Department of Otolaryngology-Head and Neck Surgery, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, Shanghai 200031, P.R. China.
authors: Liang
authors: L
authors: Zhou
keywords: 9-hydroxypheophorbide-a; cell death; diode laser; laryngeal cancer; migration