title: Betulinic Acid, The First Lupane-Type Triterpenoid Isolated from Both a Phomopsis sp. and Its Host Plant Diospyros carbonaria Benoist.
abstract: Following a biological screening using a dengue replicon virus-cell-based assay, Diospyros carbonaria AcOEt extract was investigated, affording six known lupane-type triterpenoids endowed with anti-DENV-2 NS5 polymerase activity. The study of the associated microbial community of this species permitted us to identify 38 endophytes belonging to five different orders. Nine out of these 38 strains showed significant activity on the dengue replicon assay. The chemical investigation of the most active one, Phomopsis sp. SNB-LAP1-7-32, led to the isolation of betulinic acid, an anti-viral secondary metabolite isolated previously from the host plant. This result is the first example of a lupane-type triterpenoid isolated from both an endophyte and its host plant. Its presence in the Phomopsis strain may result from gene transfer and/or specific niche selection.
authors: Institut de Chimie des Substances Naturelles, CNRS, UPR2301, University Paris-Saclay, FR-91198, Gif-sur-Yvette Cedex, France.
authors: Laure-Anne
authors: LA
authors: Peyrat
authors: Institut de Chimie des Substances Naturelles, CNRS, UPR2301, University Paris-Saclay, FR-91198, Gif-sur-Yvette Cedex, France.
authors: Véronique
authors: V
authors: Eparvier
authors: Centre de Recherche Architecture et Fonction des Macromolécules Biologiques, UMR 7257 CNRS, Aix-Marseille University, 163 Avenue de Luminy, FR-13288, Marseille Cedex 09, France.
authors: Cécilia
authors: C
authors: Eydoux
authors: Centre de Recherche Architecture et Fonction des Macromolécules Biologiques, UMR 7257 CNRS, Aix-Marseille University, 163 Avenue de Luminy, FR-13288, Marseille Cedex 09, France.
authors: Jean-Claude
authors: JC
authors: Guillemot
authors: Institut de Chimie des Substances Naturelles, CNRS, UPR2301, University Paris-Saclay, FR-91198, Gif-sur-Yvette Cedex, France.
authors: Marc
authors: M
authors: Litaudon
authors: Institut de Chimie des Substances Naturelles, CNRS, UPR2301, University Paris-Saclay, FR-91198, Gif-sur-Yvette Cedex, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique, FR-66650, Banyuls/Mer, France.
authors: Didier
authors: D
authors: Stien
mesh_terms: D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry*; D029788:Diospyros / Q000737:chemistry*; D060026:Endophytes / Q000737:chemistry; D053978:Pentacyclic Triterpenes; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D000094062:Betulinic Acid
chemical_list: D000998:Antiviral Agents; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; C480546:lupane; D000094062:Betulinic Acid
keywords: 
  Diospyros carbonaria
  ; Dengue polymerase inhibition; Phomopsis sp.; Plant-endophyte common metabolite; Triterpenoids