title: A Multicomponent Animal Virus Isolated from Mosquitoes.
abstract: RNA viruses exhibit a variety of genome organization strategies, including multicomponent genomes in which each segment is packaged separately. Although multicomponent genomes are common among viruses infecting plants and fungi, their prevalence among those infecting animals remains unclear. We characterize a multicomponent RNA virus isolated from mosquitoes, designated Guaico Culex virus (GCXV). GCXV belongs to a diverse clade of segmented viruses (Jingmenvirus) related to the prototypically unsegmented Flaviviridae. The GCXV genome comprises five segments, each of which appears to be separately packaged. The smallest segment is not required for replication, and its presence is variable in natural infections. We also describe a variant of Jingmen tick virus, another Jingmenvirus, sequenced from a Ugandan red colobus monkey, thus expanding the host range of this segmented and likely multicomponent virus group. Collectively, this study provides evidence for the existence of multicomponent animal viruses and their potential relevance for animal and human health.
authors: Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. Electronic address: jason.t.ladner.ctr@mail.mil.
authors: Jason T
authors: JT
authors: Ladner
authors: Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: Michael R
authors: MR
authors: Wiley
authors: Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: Brett
authors: B
authors: Beitzel
authors: Institute for Human Infections and Immunity, Departments of Pathology, Microbiology & Immunology, and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Albert J
authors: AJ
authors: Auguste
authors: Arbovirus Laboratory, Wadsworth Center, New York State Department of Health, Albany, NY 12159, USA.
authors: Alan P
authors: AP
authors: Dupuis
authors: Virology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: Michael E
authors: ME
authors: Lindquist
authors: Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors: Samuel D
authors: SD
authors: Sibley
authors: Molecular and Translational Sciences Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: Krishna P
authors: KP
authors: Kota
authors: Research Support Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: David
authors: D
authors: Fetterer
authors: Arbovirus Laboratory, Wadsworth Center, New York State Department of Health, Albany, NY 12159, USA.
authors: Gillian
authors: G
authors: Eastwood
authors: Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: David
authors: D
authors: Kimmel
authors: Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: Karla
authors: K
authors: Prieto
authors: Institute for Human Infections and Immunity, Departments of Pathology, Microbiology & Immunology, and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Hilda
authors: H
authors: Guzman
authors: Arbovirus Laboratory, Wadsworth Center, New York State Department of Health, Albany, NY 12159, USA.
authors: Matthew T
authors: MT
authors: Aliota
authors: Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: Daniel
authors: D
authors: Reyes
authors: Molecular and Translational Sciences Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: Ernst E
authors: EE
authors: Brueggemann
authors: Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: Lena
authors: L
authors: St John
authors: Makerere University, Kampala, Uganda.
authors: David
authors: D
authors: Hyeroba
authors: Wisconsin National Primate Research Center, Madison, WI 53715, USA; Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA.
authors: Michael
authors: M
authors: Lauck
authors: Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA; Wisconsin National Primate Research Center, Madison, WI 53715, USA.
authors: Thomas C
authors: TC
authors: Friedrich
authors: Wisconsin National Primate Research Center, Madison, WI 53715, USA; Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA.
authors: David H
authors: DH
authors: O'Connor
authors: Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: Marie C
authors: MC
authors: Gestole
authors: Molecular and Translational Sciences Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA; Henry M. Jackson Foundation, Bethesda, MD 20817, USA; DoD Biotechnology High Performance Computing Software Applications Institute, Frederick, MD 21702, USA; Telemedicine and Advanced Technology Research Center, U.S. Army Medical Research and Materiel Command, Fort Detrick, MD 21702, USA.
authors: Lisa H
authors: LH
authors: Cazares
authors: Institute for Human Infections and Immunity, Departments of Pathology, Microbiology & Immunology, and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Vsevolod L
authors: VL
authors: Popov
authors: U.S. Naval Medical Research Unit No. 6, Lima, Peru.
authors: Fanny
authors: F
authors: Castro-Llanos
authors: U.S. Naval Medical Research Unit No. 6, Lima, Peru.
authors: Tadeusz J
authors: TJ
authors: Kochel
authors: Molecular and Translational Sciences Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: Tara
authors: T
authors: Kenny
authors: Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: Bailey
authors: B
authors: White
authors: Molecular and Translational Sciences Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
authors: Michael D
authors: MD
authors: Ward
authors: Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología, Ciudad de Panamá, Panamá.
authors: Jose R
authors: JR
authors: Loaiza
authors: Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA; Makerere University, Kampala, Uganda; Wisconsin National Primate Research Center, Madison, WI 53715, USA.
authors: Tony L
authors: TL
authors: Goldberg
authors: Institute for Human Infections and Immunity, Departments of Pathology, Microbiology & Immunology, and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Scott C
authors: SC
authors: Weaver
authors: Arbovirus Laboratory, Wadsworth Center, New York State Department of Health, Albany, NY 12159, USA; School of Public Health, State University of New York at Albany, One University Place Rensselaer, East Greenbush, NY 12144, USA.
authors: Laura D
authors: LD
authors: Kramer
authors: Institute for Human Infections and Immunity, Departments of Pathology, Microbiology & Immunology, and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Robert B
authors: RB
authors: Tesh
authors: Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA. Electronic address: gustavo.f.palacios.ctr@mail.mil.
authors: Gustavo
authors: G
authors: Palacios
mesh_terms: D000818:Animals; D003104:Colobus / Q000821:virology*; D009033:Culicidae / Q000821:virology*; D008856:Microscopy, Fluorescence; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure*; D014780:Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure*