title: Laser Capture Microdissection as a Tool to Study Tumor Stroma.
abstract: Laser capture microdissection (or LCM) allows for isolation of cells from specific tissue compartments, which can then be followed by DNA, RNA, and/or protein isolation and downstream characterization. Unlike other methods for cell isolation, LCM can be directed towards cells situated in specific anatomical contexts, and is therefore of significant value when investigating the tumor microenvironment, where localization is often key to function. Here, we present a summary of ways in which LCM can be utilized, as well as protocols for the isolation of tumor and tumor-associated stromal elements from frozen breast cancer samples, with a focus on preparation of samples for RNA characterization.
authors: Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montréal, QC, Canada.
authors: Nicholas R
authors: NR
authors: Bertos
authors: Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montréal, QC, Canada. morag.park@mcgill.ca.|Department of Experimental Medicine, McGill University, Montréal, QC, Canada. morag.park@mcgill.ca.|Department of Biochemistry, McGill University, Montréal, QC, Canada. morag.park@mcgill.ca.|Department of Oncology, McGill University, Montréal, QC, Canada. morag.park@mcgill.ca.|Department of Pathology, McGill University, Montréal, QC, Canada. morag.park@mcgill.ca.
authors: Morag
authors: M
authors: Park
mesh_terms: D015415:Biomarkers; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology*
chemical_list: D015415:Biomarkers
keywords: Arcturus PixCell IIe; Cell isolation; Laser capture microdissection; Quality control; RNA profiling