title: Fluorometric immunoassay for human serum albumin based on its inhibitory effect on the immunoaggregation of quantum dots with silver nanoparticles.
abstract: Quantitative determination of HSA was conducted by competitive immunoassay. Inhibition of aggregation of antibody conjugated quantum dots (QD) with albumin conjugated silver nanoparticles (AgNPs) in the presence of HSA was conducted. If antibody-loaded CdSe QDs aggregate with HSA-coated silver nanoparticles the distance between the two kinds of nanoparticles will be reduced enough to cause fluorescence resonance energy transfer (FRET). In this case the yellow fluorescence of the Ab-QDs is quenched. However if HSA (antigen) is added to the Ab-QDs their surface will be blocked and they cannot aggregate any longer with the HSA-AgNPs. Hence, fluorescence will not be quenched. The drop of the intensity of fluorescence (peaking at 570nm) is inversely correlated with the concentration of HSA in the sample. The method allows to determine HSA in the 30-600ng·mL-1concentration range.
authors: Institute of Biochemistry, National Academy of Science, Republic of Armenia, P. Sevak str. 5/1, Yerevan 0014, Armenia.
authors: Seda S
authors: SS
authors: Marukhyan
authors: Institute of Biochemistry, National Academy of Science, Republic of Armenia, P. Sevak str. 5/1, Yerevan 0014, Armenia. Electronic address: vgasparyan@excite.com.
authors: Vardan K
authors: VK
authors: Gasparyan
mesh_terms: D016880:Anisotropy; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D000075462:Serum Albumin, Human / Q000032:analysis* / Q000737:chemistry; D012834:Silver / Q000737:chemistry
chemical_list: D012834:Silver; D000075462:Serum Albumin, Human
keywords: Albumin determination; Fluorescence energy transfer; Quantum dots; Silver nanoparticles