title: Transcriptome Characterization of Dendrolimus punctatus and Expression Profiles at Different Developmental Stages.
abstract: The pine moth Dendrolimus punctatus (Walker) is a common insect pest that confers serious damage to conifer forests in south of China. Extensive physiology and ecology studies on D. punctatus have been carried out, but the lack of genetic information has limited our understanding of the molecular mechanisms behind its development and resistance. Using RNA-seq approach, we characterized the transcriptome of this pine moth and investigated its developmental expression profiles during egg, larval, pupal, and adult stages. A total of 107.6 million raw reads were generated that were assembled into 70,664 unigenes. More than 30% unigenes were annotated by searching for homology in protein databases. To better understand the process of metamorphosis, we pairwise compared four developmental phases and obtained 17,624 differential expression genes. Functional enrichment analysis of differentially expressed genes showed positive correlation with specific physiological activities of each stage, and these results were confirmed by qRT-PCR experiments. This study provides a valuable genomic resource of D. punctatus covering all its developmental stages, and will promote future studies on biological processes at the molecular level.
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: Cong-Hui
authors: CH
authors: Yang
authors: Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China.
authors: Peng-Cheng
authors: PC
authors: Yang
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: Jing
authors: J
authors: Li
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: Fan
authors: F
authors: Yang
authors: College of Life Sciences, Capital Normal University, Beijing, China.
authors: Ai-Bing
authors: AB
authors: Zhang
mesh_terms: D000818:Animals; D002681:China; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D016678:Genome; D019476:Insect Proteins / Q000235:genetics; D007814:Larva / Q000235:genetics; D008297:Male; D008675:Metamorphosis, Biological; D058977:Molecular Sequence Annotation; D009036:Moths / Q000235:genetics* / Q000254:growth & development; D011679:Pupa / Q000235:genetics; D017423:Sequence Analysis, RNA; D013997:Time Factors; D059467:Transcriptome
chemical_list: D019476:Insect Proteins