title: Radiographic and histological evaluation of ectopic application of deproteinized bovine bone matrix.
abstract: OBJECTIVE
  To evaluate, through radiographic and histological analysis, the tissue reaction induced by a biomaterial based on deproteinized bovine bone matrix (DBBM) in the muscle of sheep.
  
  
  METHODS
  Sixteen sheep were used. The animals underwent surgery to insert polyethylene tubes containing the biomaterial in the muscle of the lower back (ectopic site) and were euthanized after 3 and 6 months. Each sheep received three tubes: Group 1 - sham group (negative control - tube without biomaterial), Group 2 - particulate autogenous bone (positive control), and Group 3 - DBBM biomaterial (GenOx Inorg). The material removed was evaluated by radiographic, macroscopic, and microscopic analysis, descriptively.
  
  
  RESULTS
  Macroscopic analysis showed that Group 3 had a greater tissue volume maintenance. Microscopic analysis indicated that Group 1 had a higher concentration of dense, thin collagen fibers (3 and 6 months); in Group 2, there was a decrease in the inflammatory process and the deposition of dense, thin collagen fibers (3 and 6 months); in Group 3, the presence of a dense connective tissue was noted, in which the DBBM particles (3 months) were found. On the periphery of these particles, a deposition of basophilic material was found, indicating the formation of mineral particles and the formation of tissues with osteoid characteristics (6 months).
  
  
  CONCLUSIONS
  Based on the results obtained, it can be concluded that the biomaterial based on DBBM led to the formation of tissue with similar characteristics to an osteoid matrix in a postoperative period of 6 months. However, none of the groups evaluated showed ectopic bone neoformation.
authors: Graduate Program in Dentistry, Positivo University, Curitiba, PR, Brazil.
authors: Rodrigo Carlos
authors: RC
authors: da Silva
authors: Graduate Program in Dentistry, Positivo University, Curitiba, PR, Brazil.
authors: Viviane Rozeira
authors: VR
authors: Crivellaro
authors: Graduate Program in Dentistry, Positivo University, Curitiba, PR, Brazil.
authors: Allan Fernando
authors: AF
authors: Giovanini
authors: Graduate Program in Dentistry, Positivo University, Curitiba, PR, Brazil.
authors: Rafaela
authors: R
authors: Scariot
authors: Graduate Program in Dentistry, Positivo University, Curitiba, PR, Brazil.
authors: Carla Castiglia
authors: CC
authors: Gonzaga
authors: Graduate Program in Dentistry, Positivo University, Curitiba, PR, Brazil.
authors: João César
authors: JC
authors: Zielak
keywords: Bone matrix; ectopic graft; sheep