title: Nogo receptor 1 is expressed in both primary cultured glial cells and neurons.
abstract: Nogo receptor (NgR) is common in myelin-derived molecules, i.e., Nogo, MAG, and OMgp, and plays important roles in both axon fasciculation and the inhibition of axonal regeneration. In contrast to NgR's roles in neurons, its roles in glial cells have been poorly explored. Here, we found a dynamic regulation of NgR1 expression during development and neuronal injury. NgR1 mRNA was consistently expressed in the brain from embryonic day 18 to postnatal day 25. In contrast, its expression significantly decreased in the spinal cord during development. Primary cultured neurons, microglia, and astrocytes expressed NgR1. Interestingly, a contusion injury in the spinal cord led to elevated NgR1 mRNA expression at the injury site, but not in the motor cortex, 14 days after injury. Consistent with this, astrocyte activation by TGFβ1 increased NgR1 expression, while microglia activation rather decreased NgR1 expression. These results collectively suggest that NgR1 expression is enhanced in a milieu of neural injury. Our findings may provide insight into the roles of NgR1 in glial cells.
authors: Departments of Biochemistry, Nagoya University Graduate School of Medicine, Nagoya, Japan; Departments of Orthopedic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Junichi
authors: J
authors: Ukai
authors: Departments of Orthopedic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Shiro
authors: S
authors: Imagama
authors: Departments of Orthopedic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan; Department of Developmental Molecular Anatomy, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan.
authors: Tomohiro
authors: T
authors: Ohgomori
authors: Departments of Orthopedic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Zenya
authors: Z
authors: Ito
authors: Departments of Orthopedic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Kei
authors: K
authors: Ando
authors: Departments of Orthopedic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Naoki
authors: N
authors: Ishiguro
authors: Departments of Biochemistry, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors: Kenji
authors: K
authors: Kadomatsu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D009457:Neuroglia; D009474:Neurons; D000070816:Nogo Receptor 1; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000070816:Nogo Receptor 1
keywords: NgR1; Spinal cord injury; astrocytes