title: Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216(T).
abstract: Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of the B. smithii type strain DSM 4216(T), which consists of a 3,368,778 bp chromosome (GenBank accession number CP012024.1) and a 12,514 bp plasmid (GenBank accession number CP012025.1), together encoding 3880 genes. Genome annotation via RAST was complemented by a protein domain analysis. Some unique features of B. smithii central metabolism in comparison to related organisms included the lack of a standard acetate production pathway with no apparent pyruvate formate lyase, phosphotransacetylase, and acetate kinase genes, while acetate was the second fermentation product.
authors: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands ; Present address: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Kgs. Lyngby, 2800 Denmark.
authors: Elleke F
authors: EF
authors: Bosma
authors: Laboratory of Systems and Synthetic Biology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors: Jasper J
authors: JJ
authors: Koehorst
authors: CMBI, NCMLS, Geert-Grooteplein Zuid 26-28, 6525 GA Nijmegen, The Netherlands.
authors: Sacha A F T
authors: SA
authors: van Hijum
authors: CMBI, NCMLS, Geert-Grooteplein Zuid 26-28, 6525 GA Nijmegen, The Netherlands.
authors: Bernadet
authors: B
authors: Renckens
authors: Corbion, Arkelsedijk 46, 4206 AC Gorinchem, The Netherlands.
authors: Bastienne
authors: B
authors: Vriesendorp
authors: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors: Antonius H P
authors: AH
authors: van de Weijer
authors: Laboratory of Systems and Synthetic Biology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors: Peter J
authors: PJ
authors: Schaap
authors: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors: Willem M
authors: WM
authors: de Vos
authors: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors: John
authors: J
authors: van der Oost
authors: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands ; Corbion, Arkelsedijk 46, 4206 AC Gorinchem, The Netherlands.
authors: Richard
authors: 0000-0001-5856-1546
authors: R
authors: van Kranenburg
keywords: Bacillus smithii; Biotechnology; Genome sequence; Lactic acid; Thermophile; Thermophilic bacillus