title: High-affinity monoclonal IgA regulates gut microbiota and prevents colitis in mice.
abstract: Immunoglobulin A (IgA) is the main antibody isotype secreted into the intestinal lumen. IgA plays a critical role in the defence against pathogens and in the maintenance of intestinal homeostasis. However, how secreted IgA regulates gut microbiota is not completely understood. In this study, we isolated monoclonal IgA antibodies from the small intestine of healthy mouse. As a candidate for an efficient gut microbiota modulator, we selected a W27 IgA, which binds to multiple bacteria, but not beneficial ones such as Lactobacillus casei. W27 could suppress the cell growth of Escherichia coli but not L. casei in vitro, indicating an ability to improve the intestinal environment. Indeed W27 oral treatment could modulate gut microbiota composition and have a therapeutic effect on both lymphoproliferative disease and colitis models in mice. Thus, W27 IgA oral treatment is a potential remedy for inflammatory bowel disease, acting through restoration of host-microbial symbiosis.
authors: Department of Immunology, Nagahama Institute of Bioscience and Technology, Nagahama, Shiga 526-0829, Japan.
authors: Shinsaku
authors: S
authors: Okai
authors: Department of Immunology, Nagahama Institute of Bioscience and Technology, Nagahama, Shiga 526-0829, Japan.
authors: Fumihito
authors: F
authors: Usui
authors: Department of Immunology, Nagahama Institute of Bioscience and Technology, Nagahama, Shiga 526-0829, Japan.
authors: Shuhei
authors: S
authors: Yokota
authors: Department of Immunology, Nagahama Institute of Bioscience and Technology, Nagahama, Shiga 526-0829, Japan.
authors: Yusaku
authors: Y
authors: Hori-I
authors: Department of Protein Function Analysis, Nagahama Institute of Bioscience and Technology, Nagahama, Shiga 526-0829, Japan.
authors: Makoto
authors: M
authors: Hasegawa
authors: Department of Epigenetics, Nagahama Institute of Bioscience and Technology, Nagahama, Shiga 526-0829, Japan.
authors: Toshinobu
authors: http://orcid.org/0000-0003-3881-3368
authors: T
authors: Nakamura
authors: Department of Diagnostic Pathology, Kyoto University Hospital, Kyoto 606-8501, Japan.
authors: Manabu
authors: M
authors: Kurosawa
authors: Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, Kumamoto 860-0811, Japan.
authors: Seiji
authors: S
authors: Okada
authors: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Tokyo 152-8550, Japan.
authors: Kazuya
authors: K
authors: Yamamoto
authors: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Tokyo 152-8550, Japan.
authors: Eri
authors: E
authors: Nishiyama
authors: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Tokyo 152-8550, Japan.
authors: Hiroshi
authors: H
authors: Mori
authors: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Tokyo 152-8550, Japan.
authors: Takuji
authors: T
authors: Yamada
authors: Earth-Life Science Institute, Tokyo Institute of Technology, Tokyo 152-8550, Japan.
authors: Ken
authors: http://orcid.org/0000-0001-8662-9958
authors: K
authors: Kurokawa
authors: Yakult Central Institute, Tokyo 186-8650, Japan.
authors: Satoshi
authors: S
authors: Matsumoto
authors: Yakult Central Institute, Tokyo 186-8650, Japan.
authors: Masanobu
authors: M
authors: Nanno
authors: Yakult Central Institute, Tokyo 186-8650, Japan.
authors: Tomoaki
authors: T
authors: Naito
authors: Yakult Central Institute, Tokyo 186-8650, Japan.
authors: Yohei
authors: Y
authors: Watanabe
authors: RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa 230-0045, Japan.
authors: Tamotsu
authors: T
authors: Kato
authors: RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa 230-0045, Japan.
authors: Eiji
authors: E
authors: Miyauchi
authors: RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa 230-0045, Japan.|Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.|Graduate School of Medical Life Science, Yokohama City University, Kanagawa 230-0045, Japan.
authors: Hiroshi
authors: http://orcid.org/0000-0001-8776-9661
authors: H
authors: Ohno
authors: Department of Immunology, Nagahama Institute of Bioscience and Technology, Nagahama, Shiga 526-0829, Japan.|PRESTO, Japan Science and Technology Agency, Saitama 332-0012, Japan.
authors: Reiko
authors: R
authors: Shinkura
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003092:Colitis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D004926:Escherichia coli / Q000254:growth & development / Q000276:immunology; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000276:immunology*; D006706:Homeostasis; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D015212:Inflammatory Bowel Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D007421:Intestine, Small / Q000276:immunology / Q000382:microbiology; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013559:Symbiosis
chemical_list: D000911:Antibodies, Monoclonal; D007071:Immunoglobulin A, Secretory