title: Identification of the First Riboflavin Catabolic Gene Cluster Isolated from Microbacterium maritypicum G10.
abstract: Riboflavin is a common cofactor, and its biosynthetic pathway is well characterized. However, its catabolic pathway, despite intriguing hints in a few distinct organisms, has never been established. This article describes the isolation of a Microbacterium maritypicum riboflavin catabolic strain, and the cloning of the riboflavin catabolic genes. RcaA, RcaB, RcaD, and RcaE were overexpressed and biochemically characterized as riboflavin kinase, riboflavin reductase, ribokinase, and riboflavin hydrolase, respectively. Based on these activities, a pathway for riboflavin catabolism is proposed.
authors: From the Department of Chemistry, Texas A&M University, College Station, Texas 77843.
authors: Hui
authors: H
authors: Xu
authors: From the Department of Chemistry, Texas A&M University, College Station, Texas 77843.
authors: Yindrila
authors: Y
authors: Chakrabarty
authors: From the Department of Chemistry, Texas A&M University, College Station, Texas 77843.
authors: Benjamin
authors: B
authors: Philmus
authors: From the Department of Chemistry, Texas A&M University, College Station, Texas 77843.
authors: Angad P
authors: AP
authors: Mehta
authors: From the Department of Chemistry, Texas A&M University, College Station, Texas 77843.
authors: Dhananjay
authors: D
authors: Bhandari
authors: From the Department of Chemistry, Texas A&M University, College Station, Texas 77843.
authors: Hans-Peter
authors: HP
authors: Hohmann
authors: From the Department of Chemistry, Texas A&M University, College Station, Texas 77843 begley@tamu.edu.
authors: Tadhg P
authors: TP
authors: Begley
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D038181:FMN Reductase / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D012256:Riboflavin / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D038181:FMN Reductase; D017853:Phosphotransferases (Alcohol Group Acceptor); C022534:ribokinase; C022532:riboflavin kinase; D006867:Hydrolases; D012256:Riboflavin
keywords: Vitamin B2; catabolism; cloning; enzyme mechanism; flavoprotein; lyase; quorum sensing; reconstitution; riboflavin