title: A Selenium-Enriched Ziyang Green Tea Polysaccharide Induces Bax-Dependent Mitochondrial Apoptosis and Inhibits TGF-β1-Stimulated Collagen Expression in Human Keloid Fibroblasts via NG2 Inactivation.
abstract: Keloids are fibroproliferative disorders characterized by the overabundant deposition of extracellular matrix (ECM), especially collagen and overgrowth of scar tissue in response to cutaneous injury. In this study, we isolated a selenium (Se)-containing polysaccharide (Se-ZGTP-I) from Ziyang green tea and explored its potential therapeutic effects on keloid fibroblasts formation. 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and annexin V/propidium iodide (PI) staining assays demonstrated that Se-ZGTP-I or neuron-glia 2 (NG2) short hairpin RNA (shRNA) significantly inhibited proliferation of human keloid fibroblasts via induction of apoptosis. Besides, the activation of caspase-3 and the subsequent cleavage of poly (ADP-ribose) polymerase (PARP) were observed in keloid fibroblasts following Se-ZGTP-I (200 and 400 μg/ml) or NG2 shRNA treatment. Moreover, Western blotting analysis showed that treatment of keloid fibroblasts with Se-ZGTP-I (200 and 400 μg/ml) or NG2 shRNA resulted in an increase of pro-apoptotic protein Bax expression and a decrease in expression levels of anti-apoptotic protein Bcl-2 and NG2. In addition, type I collagen biosynthesis and protein expression in keloid fibroblasts following TGF-β1 stimulation were decreased by Se-ZGTP-I (200 and 400 μg/ml) or NG2 shRNA management. Current findings imply that Se-ZGTP-I has a therapeutic potential to intervene and prevent keloid formation and other fibrotic diseases.
authors: The Third Military Medical University Southwest Plastic Surgery Hospital, Chongqing, 400037, People's Republic of China.
authors: Lele
authors: L
authors: Lu
authors: The Third Military Medical University Southwest Plastic Surgery Hospital, Chongqing, 400037, People's Republic of China.
authors: Linlin
authors: L
authors: Chai
authors: The Third Military Medical University Southwest Plastic Surgery Hospital, Chongqing, 400037, People's Republic of China.
authors: Wenping
authors: W
authors: Wang
authors: The Third Military Medical University Southwest Plastic Surgery Hospital, Chongqing, 400037, People's Republic of China.
authors: Xi
authors: X
authors: Yuan
authors: The Third Military Medical University Southwest Plastic Surgery Hospital, Chongqing, 400037, People's Republic of China. shirong_li@aol.com.
authors: Shirong
authors: S
authors: Li
authors: The Third Military Medical University Southwest Plastic Surgery Hospital, Chongqing, 400037, People's Republic of China. chuancaotmmu@sina.cn.
authors: Chuan
authors: C
authors: Cao
mesh_terms: D000941:Antigens; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011509:Proteoglycans / Q000037:antagonists & inhibitors*; D012643:Selenium / Q000737:chemistry / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry*; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism*; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D003094:Collagen; D011134:Polysaccharides; D011509:Proteoglycans; D012643:Selenium; D013662:Tea; D053773:Transforming Growth Factor beta1; D051028:bcl-2-Associated X Protein; D000099216:Chondroitin Sulfate Proteoglycan 4; C494826:BAX protein, human
keywords: Collagen; Keloid; NG2; TGF-β1; Ziyang green tea polysaccharides