title: Dystroglycan Suppresses Notch to Regulate Stem Cell Niche Structure and Function in the Developing Postnatal Subventricular Zone.
abstract: While the extracellular matrix (ECM) is known to regulate neural stem cell quiescence in the adult subventricular zone (SVZ), the function of ECM in the developing SVZ remains unknown. Here, we report that the ECM receptor dystroglycan regulates a unique developmental restructuring of ECM in the early postnatal SVZ. Dystroglycan is furthermore required for ependymal cell differentiation and assembly of niche pinwheel structures, at least in part by suppressing Notch activation in radial glial cells, which leads to the increased expression of MCI, Myb, and FoxJ1, transcriptional regulators necessary for acquisition of the multiciliated phenotype. Dystroglycan also regulates perinatal radial glial cell proliferation and transition into intermediate gliogenic progenitors, such that either acute or constitutive loss of function in dystroglycan results in increased oligodendrogenesis. These findings reveal a role for dystroglycan in orchestrating both the assembly and function of the SVZ neural stem cell niche.
authors: Department of Pharmacology, Stony Brook University, Stony Brook, NY 11794-8651, USA.
authors: Freyja K
authors: FK
authors: McClenahan
authors: Department of Pharmacology, Stony Brook University, Stony Brook, NY 11794-8651, USA.
authors: Himanshu
authors: H
authors: Sharma
authors: Department of Pharmacology, Stony Brook University, Stony Brook, NY 11794-8651, USA.
authors: Xiwei
authors: X
authors: Shan
authors: Department of Pharmacology, Stony Brook University, Stony Brook, NY 11794-8651, USA.
authors: Christopher
authors: C
authors: Eyermann
authors: Department of Pharmacology, Stony Brook University, Stony Brook, NY 11794-8651, USA. Electronic address: holly.colognato@stonybrook.edu.
authors: Holly
authors: H
authors: Colognato
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D049030:Dystroglycans / Q000235:genetics* / Q000378:metabolism; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism; D020547:Lateral Ventricles / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D058953:Neural Stem Cells / Q000378:metabolism*; D055495:Neurogenesis / Q000235:genetics*; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D051381:Rats; D051880:Receptors, Notch / Q000096:biosynthesis / Q000235:genetics; D055153:Stem Cell Niche / Q000235:genetics*
chemical_list: D051880:Receptors, Notch; D049030:Dystroglycans