title: Pooled shRNA Screening in Mammalian Cells as a Functional Genomic Discovery Platform.
abstract: Functional genomic screens using shRNA technology are a great tool in biomedical research. As more labs gain access to the necessary reagents and technology to perform such screens, some may lack in-depth knowledge on the difficulties often encountered. With this protocol, we aim to point out the most important caveats of performing shRNA based screens and provide a streamlined workflow that can be easily adapted to meet the specific needs of any particular screening project.
authors: Division of Molecular Carcinogenesis and NKI Robotics and Screening Center, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.
authors: Katarzyna
authors: K
authors: Jastrzebski
authors: Division of Molecular Carcinogenesis and NKI Robotics and Screening Center, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.
authors: Bastiaan
authors: B
authors: Evers
authors: Division of Molecular Carcinogenesis and NKI Robotics and Screening Center, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands. r.beijersbergen@nki.nl.
authors: Roderick L
authors: RL
authors: Beijersbergen
mesh_terms: D000818:Animals; D002460:Cell Line; D015723:Gene Library; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008322:Mammals / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D034741:RNA, Small Interfering / Q000032:analysis / Q000235:genetics*; D014161:Transduction, Genetic; D057188:Workflow
chemical_list: D034741:RNA, Small Interfering
keywords: Functional genetics; RNAi; Screens; shRNA