title: [miR-124 regulates radiosensitivity of colorectal cancer cells by targeting PRRX1].
abstract: OBJECTIVE
  To detect the expression of miR-124 in colorectal carcinoma (CRC) cells and tissue specimens and analyze its association with the radiosensitivity of the cells.
  
  
  METHODS
  The expression of miR-124 in CRC cell lines and tissues were detected using qRT-PCR. The effect of miR-124 in modulating cell radiosensitivity was assessed in CRC cells with miRNA-124 overexpression and miRNA-124 knockdown, and bioinformatics prediction and dual luciferase reporter system were employed to identify the direct target of miR-124.
  
  
  RESULTS
  s miR-124 expression was down-regulated in CRC cell lines and tissues. CRC cells over-expressing miR-124 showed an obviously enhanced radiosensitivity, whereas miR-124 knockdown resulted in a reduced radiosensitivity of the cells. Bioinformatics prediction and dual luciferase reporter system verified PRRX1 as a direct target of miR-124, which regulated the radiosensitivity of CRC cells by directly inhibiting PRRX1.
  
  
  CONCLUSIONS
  miR-124 can enhance the radiosensitivity of CRC cells by directly targeting PRRX1, which provides a target for improving the therapeutic effect of radiotherapy of CRC.
authors: Department of Radiation Oncology, Nanfang Hospital1, Department of Pathology, Southern Medical University, Guangzhou 510515, China. E-mail: 172621156@qq.com.
authors: Shui-Miao
authors: SM
authors: Lin
authors: Qiong
authors: Q
authors: Xia
authors: Yu-Qin
authors: YQ
authors: Zhang
authors: Ai-Min
authors: AM
authors: Sun
authors: Yu-Sheng
authors: YS
authors: Shi
authors: Lin
authors: L
authors: Zheng
authors: Long-Hua
authors: LH
authors: Chen
mesh_terms: D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000473:pathology* / Q000532:radiotherapy; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008156:Luciferases; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011836:Radiation Tolerance
chemical_list: D018398:Homeodomain Proteins; C509207:MIRN124 microRNA, human; D035683:MicroRNAs; C082157:PRRX1 protein, human; D008156:Luciferases