title: A quantitative method for determining relative colonization rates of maize callus by Fusarium graminearum for resistance gene evaluations.
abstract: A quantitative PCR method was developed for detecting Fusarium graminearum growing in maize callus. Fungal DNA was detected 12h after inoculation (detection limit, 0.2pg) and was correlated with visual ratings. The method effectively quantified fungal growth in callus overexpressing a peroxidase gene conferring fungal resistance.
authors: Crop Bioprotection Research, USDA Agricultural Research Service, 1815 N. University Street, Peoria, IL 61604, United States. Electronic address: eric.johnson2@ars.usda.gov.
authors: Eric T
authors: ET
authors: Johnson
authors: Crop Bioprotection Research, USDA Agricultural Research Service, 1815 N. University Street, Peoria, IL 61604, United States.
authors: Patrick F
authors: PF
authors: Dowd
mesh_terms: D002146:Bony Callus / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis*; D018744:DNA, Plant / Q000032:analysis; D005670:Fusarium / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D015966:Gene Expression Regulation, Fungal; D019450:Genes, MDR / Q000235:genetics; D016533:Mycological Typing Techniques / Q000379:methods; D009183:Mycotoxins / Q000032:analysis; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D013172:Spores, Fungal; D003313:Zea mays / Q000235:genetics* / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D018744:DNA, Plant; D009183:Mycotoxins; D010940:Plant Proteins; D009195:Peroxidase
keywords: Callus; Maize; Mycotoxin; Pathogen resistance; Peroxidase