title: Identification of Atg8 from Acanthamoeba castellanii by genetic complementation in Saccharomyces cerevisiae.
abstract: Autophagy is a eukaryotic process responsible for the degradation of intracellular content such as damaged organelles. Several putative autophagy-related genes have been identified within the annotated genome of the free-living amoeba Acanthamoeba castellanii. However, the involvement of the corresponding proteins in the autophagy pathway had not been formerly established. Here, we report that AcAtg8 cDNA can complement ATG8-deficient Saccharomyces cerevisiae.
authors: Signalisation et Transports Ioniques Membranaires, CNRS ERL 7368, Université de Poitiers, Bât B31, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France.
authors: Matthieu
authors: M
authors: Régnacq
authors: Signalisation et Transports Ioniques Membranaires, CNRS ERL 7368, Université de Poitiers, Bât B31, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France.
authors: Pierre
authors: P
authors: Voisin
authors: Laboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS 7267, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France.
authors: Yann
authors: Y
authors: Héchard
authors: Signalisation et Transports Ioniques Membranaires, CNRS ERL 7368, Université de Poitiers, Bât B31, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France.
authors: Thierry
authors: T
authors: Bergès
authors: Laboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS 7267, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France.
authors: Christine
authors: C
authors: Braquart-Varnier
authors: Laboratoire Ecologie et Biologie des Interactions, Université de Poitiers, UMR CNRS 7267, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France. Electronic address: ascel.samba@univ-poitiers.fr.
authors: Ascel
authors: A
authors: Samba-Louaka
mesh_terms: D048248:Acanthamoeba castellanii / Q000235:genetics* / Q000378:metabolism; D001343:Autophagy / Q000235:genetics; D000071190:Autophagy-Related Protein 8 Family / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005816:Genetic Complementation Test; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D000071190:Autophagy-Related Protein 8 Family; D015800:Protozoan Proteins
keywords: Acanthamoeba castellanii; Atg8; Autophagy; Saccharomyces cerevisiae