title: Visualizing Wnt Palmitoylation in Single Cells.
abstract: Wnt palmitoylation regulates its secretion and signaling activity in cells. Methods to monitor cellular Wnt palmitoylation are instrumental in investigating Wnt activity, secretion, and its interaction with cellular membrane compartments. This protocol describes a method we have recently developed to detect cellular Wnt palmitoylation. The method, combining click chemistry, bio-orthogonal fatty acid probes, and proximity ligation assay (PLA), provides high sensitivity and subcellular resolution for detection of Wnt palmitoylation. It is also compatible with multiple imaging platforms, and is applicable to detecting palmitoylated forms of other fatty acylated proteins.
authors: Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390-9039, USA.
authors: Xinxin
authors: X
authors: Gao
authors: Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390-9039, USA. hannoush.rami@gene.com.
authors: Rami N
authors: RN
authors: Hannoush
mesh_terms: D000215:Acylation; D058948:Cell Tracking / Q000379:methods; D057930:Click Chemistry / Q000379:methods*; D006801:Humans; D054878:Lipoylation; D015398:Signal Transduction; D059010:Single-Cell Analysis / Q000379:methods*; D051153:Wnt Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D051153:Wnt Proteins
keywords: Alkyne fatty acid; Click chemistry; Microscopy; Proximity ligation; Wnt palmitoylation