title: Analysis of the C. elegans Nucleolus by Immuno-DNA FISH.
abstract: Caenorhabditis elegans is a well-established model organism which allows, among others, to investigate the link between nucleolar structure/function on the one hand and cell fate choices and cellular differentiation on the other. In addition, C. elegans can be used to study the role of the nucleolus in processes that can be difficult to faithfully reproduce in vitro, such as gametogenesis, disease development, and aging. Here I present two complementary techniques, immunofluorescent staining and DNA fluorescence in situ hybridization, that have been adapted to label nucleolar components at various stages of the life cycle of the worm.
authors: Institute of Cellular Biology and Pathology, First Faculty of Medicine, Charles University in Prague, Albertov 4, 128 00, Prague, Czech Republic. christian.lanctot@lf1.cuni.cz.
authors: Christian
authors: C
authors: Lanctôt
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics*; D002466:Cell Nucleolus / Q000235:genetics* / Q000378:metabolism; D004247:DNA; D015342:DNA Probes; D004275:DNA, Ribosomal / Q000235:genetics; D047108:Embryonic Development / Q000235:genetics; D005455:Fluorescent Antibody Technique; D017404:In Situ Hybridization, Fluorescence; D018613:Microscopy, Confocal
chemical_list: D015342:DNA Probes; D004275:DNA, Ribosomal; D004247:DNA
keywords: Caenorhabditis elegans; DNA FISH; Embryogenesis; Immunofluorescence; Nucleolus