title: High-Resolution Multispectral Optoacoustic Tomography of the Vascularization and Constitutive Hypoxemia of Cancerous Tumors.
abstract: Diversity of the design and alignment of illumination and ultrasonic transducers empower the fine scalability and versatility of optoacoustic imaging. In this study, we implement an innovative high-resolution optoacoustic mesoscopy for imaging the vasculature and tissue oxygenation within subcutaneous and orthotopic cancerous implants of mice in vivo through acquisition of tomographic projections over 180° at a central frequency of 24 MHz. High-resolution volumetric imaging was combined with multispectral functional measurements to resolve the exquisite inner structure and vascularization of the entire tumor mass using endogenous and exogenous optoacoustic contrast. Evidence is presented for constitutive hypoxemia within the carcinogenic tissue through analysis of the hemoglobin absorption spectra and distribution. Morphometric readouts obtained with optoacoustic mesoscopy have been verified with high-resolution ultramicroscopic studies. The findings described herein greatly extend the applications of optoacoustic mesoscopy toward structural and multispectral functional measurements of the vascularization and hemodynamics within solid tumors in vivo and are of major relevance to basic and preclinical oncological studies in small animal models.
authors: Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstr., 85764 Neuherberg, Germany; Munich School of Bioengineering, Technische Universität München (TUM), Ismaningerstr. 22, 81675 München, Germany.
authors: Andrei
authors: A
authors: Chekkoury
authors: Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstr., 85764 Neuherberg, Germany.
authors: Antonio
authors: A
authors: Nunes
authors: Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstr., 85764 Neuherberg, Germany; Munich School of Bioengineering, Technische Universität München (TUM), Ismaningerstr. 22, 81675 München, Germany; ESPCI Paris-Tech, PSL Research University, CNRS, Institut Langevin, 1 rue Jussieu, F-75005, Paris, France.
authors: Jerome
authors: J
authors: Gateau
authors: Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstr., 85764 Neuherberg, Germany; Munich School of Bioengineering, Technische Universität München (TUM), Ismaningerstr. 22, 81675 München, Germany.
authors: Panagiotis
authors: P
authors: Symvoulidis
authors: Research Unit of Analytical Pathology, Institute of Pathology, Helmholtz Zentrum München, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany.
authors: Annette
authors: A
authors: Feuchtinger
authors: Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstr., 85764 Neuherberg, Germany.
authors: Nicolas
authors: N
authors: Beziere
authors: Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstr., 85764 Neuherberg, Germany; Munich School of Bioengineering, Technische Universität München (TUM), Ismaningerstr. 22, 81675 München, Germany.
authors: Saak V
authors: SV
authors: Ovsepian
authors: Research Unit of Analytical Pathology, Institute of Pathology, Helmholtz Zentrum München, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany.
authors: Axel
authors: A
authors: Walch
authors: Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstr., 85764 Neuherberg, Germany; Munich School of Bioengineering, Technische Universität München (TUM), Ismaningerstr. 22, 81675 München, Germany. Electronic address: v.natziachristos@tum.de.
authors: Vasilis
authors: V
authors: Ntziachristos
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D000860:Hypoxia / Q000378:metabolism*; D051379:Mice; D009367:Neoplasm Staging; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging* / Q000378:metabolism*; D019047:Phantoms, Imaging; D061088:Photoacoustic Techniques; D015203:Reproducibility of Results; D014054:Tomography / Q000379:methods