title: LC-MS-MS Method for the Analysis of Miscellaneous Drugs in Wastewater During Football Games III.
abstract: Continuing our previous studies analyzing drugs of abuse in municipal wastewater, a method was developed for the analysis of miscellaneous drugs of abuse in wastewater samples using liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS). Eight drugs and metabolites were analyzed including 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrolidine (EDDP), fentanyl, norfentanyl, meperidine, normeperidine, methadone, phencyclidine and tramadol. These drugs were chosen because of their widespread abuse. Wastewater samples were collected at both the Oxford Waste Water Treatment Plant in Oxford, Mississippi (MS) and the University Wastewater Treatment Plant in University, MS. These wastewater samples were collected on weekends in which the University of Mississippi football team (colloquially the "Ole Miss Rebels" football team) held home games (Vaught-Hemingway Stadium, University, MS 38677). The collected samples were analyzed using a validated method and found to contain tramadol in 25 samples at quantifiable levels. EDDP, meperidine, normeperidine and methadone were also detected but were under the limit of quantitation.
authors: ElSohly Laboratories, Inc., 5 Industrial Park Drive, Oxford, MS 38655, USA Department of Chemistry and Biochemistry, University of Mississippi, MS 38677, USA.
authors: Brandon
authors: B
authors: Stamper
authors: ElSohly Laboratories, Inc., 5 Industrial Park Drive, Oxford, MS 38655, USA National Center for Natural Products Research, University of Mississippi, MS 38677, USA.
authors: Waseem
authors: W
authors: Gul
authors: Department of Chemistry and Biochemistry, University of Mississippi, MS 38677, USA.
authors: Murrell
authors: M
authors: Godfrey
authors: ElSohly Laboratories, Inc., 5 Industrial Park Drive, Oxford, MS 38655, USA.
authors: Shahbaz W
authors: SW
authors: Gul
authors: ElSohly Laboratories, Inc., 5 Industrial Park Drive, Oxford, MS 38655, USA National Center for Natural Products Research, University of Mississippi, MS 38677, USA Department of Pharmaceutics and Drug Delivery, University of Mississippi, MS 38677, USA elsohly@elsohly.com.
authors: Mahmoud A
authors: MA
authors: ElSohly
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D005283:Fentanyl / Q000031:analogs & derivatives / Q000032:analysis; D005538:Football; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis; D057230:Limit of Detection; D008614:Meperidine / Q000031:analogs & derivatives / Q000032:analysis; D008691:Methadone / Q000032:analysis; D008922:Mississippi; D010622:Phencyclidine / Q000032:analysis; D011759:Pyrrolidines / Q000032:analysis; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014147:Tramadol / Q000032:analysis; D062065:Wastewater / Q000032:analysis*
chemical_list: D005283:Fentanyl; D013287:Illicit Drugs; D008614:Meperidine; D008691:Methadone; D010622:Phencyclidine; D011759:Pyrrolidines; D014147:Tramadol; D062065:Wastewater; C080127:norfentanyl; C002752:normeperidine; C010724:2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine