title: Genomic and Metabolomic Profile Associated to Clustering of Cardio-Metabolic Risk Factors.
abstract: BACKGROUND
  To identify metabolomic and genomic markers associated with the presence of clustering of cardiometabolic risk factors (CMRFs) from a general population.
  
  
  RESULTS
  One thousand five hundred and two subjects, Caucasian, > 18 years, representative of the general population, were included. Blood pressure measurement, anthropometric parameters and metabolic markers were measured. Subjects were grouped according the number of CMRFs (Group 1: <2; Group 2: 2; Group 3: 3 or more CMRFs). Using SNPlex, 1251 SNPs potentially associated to clustering of three or more CMRFs were analyzed. Serum metabolomic profile was assessed by 1H NMR spectra using a Brucker Advance DRX 600 spectrometer. From the total population, 1217 (mean age 54±19, 50.6% men) with high genotyping call rate were analysed. A differential metabolomic profile, which included products from mitochondrial metabolism, extra mitochondrial metabolism, branched amino acids and fatty acid signals were observed among the three groups. The comparison of metabolomic patterns between subjects of Groups 1 to 3 for each of the genotypes associated to those subjects with three or more CMRFs revealed two SNPs, the rs174577_AA of FADS2 gene and the rs3803_TT of GATA2 transcription factor gene, with minimal or no statistically significant differences. Subjects with and without three or more CMRFs who shared the same genotype and metabolomic profile differed in the pattern of CMRFS cluster. Subjects of Group 3 and the AA genotype of the rs174577 had a lower prevalence of hypertension compared to the CC and CT genotype. In contrast, subjects of Group 3 and the TT genotype of the rs3803 polymorphism had a lower prevalence of T2DM, although they were predominantly males and had higher values of plasma creatinine.
  
  
  CONCLUSIONS
  The results of the present study add information to the metabolomics profile and to the potential impact of genetic factors on the variants of clustering of cardiometabolic risk factors.
authors: Metabolomic and Molecular Image Lab, Health Research Institute, INCLIVA, Valencia, Spain.
authors: Vannina G
authors: VG
authors: Marrachelli
authors: Genotyping and Genetic Diagnosis Unit, Health Research Institute, INCLIVA, Valencia, Spain.
authors: Pilar
authors: P
authors: Rentero
authors: Department of Nutrition, Food Science and Physiology, University of Navarra, Pamplona, Spain.
authors: María L
authors: ML
authors: Mansego
authors: Metabolomic and Molecular Image Lab, Health Research Institute, INCLIVA, Valencia, Spain.
authors: Jose Manuel
authors: JM
authors: Morales
authors: Genotyping and Genetic Diagnosis Unit, Health Research Institute, INCLIVA, Valencia, Spain.
authors: Inma
authors: I
authors: Galan
authors: Metabolomic and Molecular Image Lab, Health Research Institute, INCLIVA, Valencia, Spain.
authors: Mercedes
authors: M
authors: Pardo-Tendero
authors: INCLIVA Research Institute, University of Valencia, Valencia, Spain.
authors: Fernando
authors: F
authors: Martinez
authors: Internal Medicine, Hospital Rio Hortega, Valladolid, Spain.
authors: Juan Carlos
authors: JC
authors: Martin-Escudero
authors: INCLIVA Research Institute, University of Valencia, Valencia, Spain.
authors: Laisa
authors: L
authors: Briongos
authors: Genotyping and Genetic Diagnosis Unit, Health Research Institute, INCLIVA, Valencia, Spain.|CIBERDem, Health Institute Carlos III, Madrid, Spain.
authors: Felipe Javier
authors: FJ
authors: Chaves
authors: INCLIVA Research Institute, University of Valencia, Valencia, Spain.|CIBERObn, Health Institute Carlos III, Madrid, Spain.
authors: Josep
authors: J
authors: Redon
authors: Metabolomic and Molecular Image Lab, Health Research Institute, INCLIVA, Valencia, Spain.
authors: Daniel
authors: D
authors: Monleon
mesh_terms: D000328:Adult; D000368:Aged; D001794:Blood Pressure / Q000502:physiology; D002318:Cardiovascular Diseases / Q000235:genetics / Q000378:metabolism*; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D023281:Genomics; D005838:Genotype; D006801:Humans; D008297:Male; D008659:Metabolic Diseases / Q000235:genetics / Q000378:metabolism*; D055432:Metabolomics; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D005819:Genetic Markers