title: Construction of Nuclear Envelope Shape by a High-Genus Vesicle with Pore-Size Constraint.
abstract: Nuclear pores have an approximately uniform distribution in the nuclear envelope of most living cells. Hence, the morphology of the nuclear envelope is a spherical stomatocyte with a high genus. We have investigated the morphology of high-genus vesicles under pore-size constraint using dynamically triangulated membrane simulations. Bending-energy minimization without volume or other constraints produces a circular-cage stomatocyte, where the pores are aligned in a circular line on an oblate bud. As the pore radius is reduced, the circular-pore alignment is more stabilized than a random pore distribution on a spherical bud. However, we have clarified the conditions for the formation of a spherical stomatocyte: a small perinuclear volume, osmotic pressure within nucleoplasm, and repulsion between the pores. When area-difference elasticity is taken into account, the formation of cylindrical or budded tubules from the stomatocyte and discoidal stomatocyte is found.
authors: Institute for Solid State Physics, University of Tokyo, Kashiwa, Chiba, Japan. Electronic address: noguchi@issp.u-tokyo.ac.jp.
authors: Hiroshi
authors: H
authors: Noguchi
mesh_terms: D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D009685:Nuclear Envelope / Q000737:chemistry / Q000378:metabolism*; D009997:Osmotic Pressure; D016062:Porosity