title: Charge Transfer Dissociation of Complex Oligosaccharides: Comparison with Collision-Induced Dissociation and Extreme Ultraviolet Dissociative Photoionization.
abstract: The structural characterization of oligosaccharides still challenges the field of analytical chemistry. Tandem mass spectrometry offers many advantages toward this aim, although the generic fragmentation method (low-energy collision-induced dissociation) shows clear limitations and is often insufficient to retrieve some essential structural information on these molecules. In this work, we present the first application of helium charge transfer dissociation (He-CTD) to characterize the structure of complex oligosaccharides. We compare this method with low-energy collision-induced dissociation and extreme-ultraviolet dissociative photoionization (XUV-DPI), which was shown previously to ensure the successful characterization of complex glycans. Similarly to what could be obtained by XUV-DPI, He-CTD provides a complete description of the investigated structures by producing many informative cross-ring fragments and no ambiguous fragmentation. Unlike XUV-DPI, which is performed at a synchrotron source, He-CTD has the undeniable advantage of being implementable in a conventional benchtop ion trap in a conventional laboratory setting. Graphical Abstract ᅟ.
authors: INRA, UR1268 Biopolymers Interactions Assemblies, 44316, Nantes, France.
authors: David
authors: D
authors: Ropartz
authors: C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, 26506, USA.
authors: Pengfei
authors: P
authors: Li
authors: INRA, UR1268 Biopolymers Interactions Assemblies, 44316, Nantes, France.
authors: Mathieu
authors: M
authors: Fanuel
authors: Synchrotron SOLEIL, L'Orme des Merisiers, 91190, Gif-sur-Yvette, France.|UAR 1008 CEPIA, INRA, 44316, Nantes, France.
authors: Alexandre
authors: A
authors: Giuliani
authors: INRA, UR1268 Biopolymers Interactions Assemblies, 44316, Nantes, France. helene.rogniaux@nantes.inra.fr.
authors: Hélène
authors: H
authors: Rogniaux
authors: C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, 26506, USA.|Department of Forensic and Investigative Science, West Virginia University, Morgantown, WV, 26506-6121, USA.
authors: Glen P
authors: GP
authors: Jackson
mesh_terms: D006371:Helium; D009844:Oligosaccharides / Q000737:chemistry*; D055668:Photochemical Processes; D011134:Polysaccharides; D017356:Synchrotrons; D053719:Tandem Mass Spectrometry; D014466:Ultraviolet Rays
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides; D006371:Helium
keywords: Characterization of complex oligosaccharide structures; Extreme Ultra-violet photon activation; Fragmentation method; Helium-charge transfer dissociation; Tandem mass spectrometry