title: SOX1 is correlated to stemness state regulator SALL4 through progression and invasiveness of esophageal squamous cell carcinoma.
abstract: SOX1, as a tumor suppressor, play anti-tumorigenecity role in different cells and its expression is inhibited in a variety of cancers. The aim of this study was to evaluate SOX1 expression and its correlation with cancer stem cell (CSC) markers in ESCC. Using real time PCR, the relative comparative expression of SOX1 in 40 ESCC samples was assessed compared to related margin normal tissues, and its correlation with CSC markers including SALL4, SOX2, and MEIS1 was analyzed statistically. The results revealed significant under-expression of SOX1 in ESCC in significant correlation with different indices of poor prognosis including depth of tumor invasion (P=0.02), Stage of tumor cell progression (P=0.05), and number of involved lymph node metastasis (P=0.05). Furthermore, the under-expression of SOX1 was associated significantly with SALL4 overexpression. This study was the first to evaluate SOX1 underexpression and its association with poor prognosis in ESCC. Since correlation of SOX1 and SALL4 was detected in advanced stages of ESCC progression, as well as high invasive and aggressive tumor tissues, it may be extrapolated that SOX1 expression may have critical role in inhibition of ESCC invasiveness and aggressiveness especially in advanced stages of the disease.
authors: Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran.
authors: Abolfazl
authors: A
authors: Rad
authors: Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
authors: Saeed
authors: S
authors: Esmaeili Dizghandi
authors: Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Mohammad Reza
authors: MR
authors: Abbaszadegan
authors: Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Negin
authors: N
authors: Taghechian
authors: Clinical Research Development Units, Sabzevar University of Medical Sciences, Sabzevar, Iran.
authors: Maryam
authors: M
authors: Najafi
authors: Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran. Electronic address: forghanifard@gmail.com.
authors: Mohammad Mahdi
authors: MM
authors: Forghanifard
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018572:Disease-Free Survival; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D009367:Neoplasm Staging; D014411:Neoplastic Stem Cells; D055748:SOXB1 Transcription Factors / Q000378:metabolism*; D015996:Survival Rate; D014157:Transcription Factors / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: D009363:Neoplasm Proteins; C472575:SALL4 protein, human; C490906:SOX1 protein, human; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors
keywords: CSCs; ESCC; SALL4; SOX1; Under-expression