title: Enhanced biodegradation of PAHs by microbial consortium with different amendment and their fate in in-situ condition.
abstract: Microbial degradation is a useful tool to prevent chemical pollution in soil. In the present study, in-situ bioremediation of polyaromatic hydrocarbons (PAHs) by microbial consortium consisting of Serratia marcescens L-11, Streptomyces rochei PAH-13 and Phanerochaete chrysosporium VV-18 has been reported. In preliminary studies, the consortium degraded nearly 60-70% of PAHs in broth within 7 days under controlled conditions. The same consortium was evaluated for its competence under natural conditions by amending the soil with ammonium sulphate, paddy straw and compost. Highest microbial activity in terms of dehydrogenase, FDA hydrolase and aryl esterase was recorded on the 5(th) day. The degradation rate of PAHs significantly increased up to 56-98% within 7 days under in-situ however almost complete dissipation (83.50-100%) was observed on the 30(th) day. Among all the co-substrates evaluated, faster degradation of PAHs was observed in compost amended soil wherein fluorene, anthracene, phenanthrene and pyrene degraded with half-life of 1.71, 4.70, 2.04 and 6.14 days respectively. Different degradation products formed were also identified by GC-MS. Besides traces of parent PAHs eleven non-polar and five polar products were identified by direct and silylation reaction respectively. Various products formed indicated that consortium was capable to degrade PAHs by oxidation to mineralization.
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India.
authors: Anamika
authors: A
authors: Sharma
authors: Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi 110012, India.
authors: Shashi Bala
authors: SB
authors: Singh
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India.
authors: Richa
authors: R
authors: Sharma
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India.
authors: Priyanka
authors: P
authors: Chaudhary
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India.
authors: Alok Kumar
authors: AK
authors: Pandey
authors: Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi 110012, India.
authors: Raunaq
authors: R
authors: Ansari
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India.
authors: Venugopal
authors: V
authors: Vasudevan
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India.
authors: Anju
authors: A
authors: Arora
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India.
authors: Surender
authors: S
authors: Singh
authors: Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi 110012, India.
authors: Supradip
authors: S
authors: Saha
authors: Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India. Electronic address: latarajat@yahoo.co.in.
authors: Lata
authors: L
authors: Nain
mesh_terms: D001673:Biodegradation, Environmental; D006207:Half-Life; D006841:Hydrocarbons, Aromatic / Q000737:chemistry / Q000378:metabolism*; D059013:Microbial Consortia; D020075:Phanerochaete / Q000378:metabolism; D011083:Polycyclic Compounds / Q000737:chemistry / Q000378:metabolism*; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism
chemical_list: D006841:Hydrocarbons, Aromatic; D011083:Polycyclic Compounds; D012987:Soil; D012989:Soil Pollutants
keywords: Bioaugmentation; Bioremediation; Consortium; Phanerochaete; Streptomyces