title: Mutant IP3 receptors attenuate store-operated Ca2+ entry by destabilizing STIM-Orai interactions in Drosophila neurons.
abstract: Store-operated Ca2+entry (SOCE) occurs when loss of Ca2+from the endoplasmic reticulum (ER) stimulates the Ca2+sensor, STIM, to cluster and activate the plasma membrane Ca2+channel Orai (encoded by Olf186-F in flies). Inositol 1,4,5-trisphosphate receptors (IP3Rs, which are encoded by a single gene in flies) are assumed to regulate SOCE solely by mediating ER Ca2+release. We show that in Drosophila neurons, mutant IP3R attenuates SOCE evoked by depleting Ca2+stores with thapsigargin. In normal neurons, store depletion caused STIM and the IP3R to accumulate near the plasma membrane, association of STIM with Orai, clustering of STIM and Orai at ER-plasma-membrane junctions and activation of SOCE. These responses were attenuated in neurons with mutant IP3Rs and were rescued by overexpression of STIM with Orai. We conclude that, after depletion of Ca2+stores in Drosophila, translocation of the IP3R to ER-plasma-membrane junctions facilitates the coupling of STIM to Orai that leads to activation of SOCE.
authors: National Centre for Biological Sciences, Tata Institute for Fundamental Research, Bellary Road, Bangalore 560065, India Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK.
authors: Sumita
authors: S
authors: Chakraborty
authors: National Centre for Biological Sciences, Tata Institute for Fundamental Research, Bellary Road, Bangalore 560065, India.
authors: Bipan K
authors: BK
authors: Deb
authors: National Centre for Biological Sciences, Tata Institute for Fundamental Research, Bellary Road, Bangalore 560065, India.
authors: Tetyana
authors: 0000-0003-1304-0004
authors: T
authors: Chorna
authors: Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK.
authors: Vera
authors: V
authors: Konieczny
authors: Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK cwt1000@cam.ac.uk gaiti@ncbs.res.in.
authors: Colin W
authors: 0000-0001-7771-1044
authors: CW
authors: Taylor
authors: National Centre for Biological Sciences, Tata Institute for Fundamental Research, Bellary Road, Bangalore 560065, India cwt1000@cam.ac.uk gaiti@ncbs.res.in.
authors: Gaiti
authors: G
authors: Hasan
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism*; D008954:Models, Biological; D050505:Mutant Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D000071740:ORAI1 Protein / Q000378:metabolism*; D000071737:Stromal Interaction Molecule 1 / Q000378:metabolism*; D014714:Vertebrates / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D053496:Inositol 1,4,5-Trisphosphate Receptors; D050505:Mutant Proteins; D000071740:ORAI1 Protein; C502662:Stim protein, Drosophila; D000071737:Stromal Interaction Molecule 1; C510747:olf186-F protein, Drosophila; D002118:Calcium
keywords: Ca2+ signalling; Drosophila; IP3 receptor; Orai; STIM; Store-operated Ca2+ entry