title: Effect of Genetic Variations within the I-mfa Gene on the Growth Traits of Chinese Cattle.
abstract: I-mfa (inhibitor of the MyoD family a) is a transcription modulator that binds to MyoD family members and inhibits their transcriptional activities. It is highly expressed in the sclerotome and plays an important role in the patterning of the somite early in development. In this study, the polymorphisms of the bovine I-mfa gene were detected by polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and DNA pool sequencing methods in 541 individuals from three Chinese cattle breeds. The results showed that P3 locus had two novel complete linked single nucleotide polymorphisms (NC_007324.4:g.12284A>G and g.12331T>C), resulting in a missense mutation p.S(AGC)113G(GGC) and a synonymous mutation p.H(CAT)128H(CAC), respectively. P4 locus had a novel SNP (NC_007324.4: g.16432C>A), which resulted in a nonsense mutation p.C(TGC)241X(TGA). The statistical analyses indicated that the three SNPs, are associated with the phenotypic traits in Luxi (LX), Qinchuan (QC), and Jiaxian (JX) cattle population (P < 0.05 or P < 0.01). The mutant-type variants were superior for growth traits; the heterozygote diplotype was associated with higher growth traits compared to wild-type homozygote. Our results provide evidence that polymorphisms in the I-mfa gene are associated with growth traits and may be used for marker-assisted selection in beef cattle breeding program.
authors: a Institute of Cellular and Molecular Biology , Jiangsu Normal University , Xuzhou, Jiangsu , China.|b College of Animal Science and Technology , Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture , Yangling, Shaanxi , China.
authors: Yong-Zhen
authors: YZ
authors: Huang
authors: a Institute of Cellular and Molecular Biology , Jiangsu Normal University , Xuzhou, Jiangsu , China.
authors: Jing-Jing
authors: JJ
authors: Li
authors: a Institute of Cellular and Molecular Biology , Jiangsu Normal University , Xuzhou, Jiangsu , China.
authors: Chun-Lei
authors: CL
authors: Zhang
authors: a Institute of Cellular and Molecular Biology , Jiangsu Normal University , Xuzhou, Jiangsu , China.
authors: Xing-Tang
authors: XT
authors: Fang
authors: b College of Animal Science and Technology , Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture , Yangling, Shaanxi , China.
authors: Xian-Yong
authors: XY
authors: Lan
authors: c Bureau of Animal Husbandry of Biyang County , Biyang, Henan , China.
authors: Xing-Lei
authors: XL
authors: Qi
authors: a Institute of Cellular and Molecular Biology , Jiangsu Normal University , Xuzhou, Jiangsu , China.
authors: Hong
authors: H
authors: Chen
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D002681:China; D015810:Linkage Disequilibrium / Q000235:genetics; D018006:Myogenic Regulatory Factors / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D018006:Myogenic Regulatory Factors
keywords: Association; I-mfa; SNP; cattle; growth traits