title: Development of stress tolerant Saccharomyces cerevisiae strains by metabolic engineering: New aspects from cell flocculation and zinc supplementation.
abstract: Budding yeast Saccharomyces cerevisiae is widely studied for the production of biofuels from lignocellulosic biomass. However, economic production is currently challenged by the repression of cell growth and compromised fermentation performance of S. cerevisiae strains in the presence of various environmental stresses, including toxic level of final products, inhibitory compounds released from the pretreatment of cellulosic feedstocks, high temperature, and so on. Therefore, it is important to improve stress tolerance of S. cerevisiae to these stressful conditions to achieve efficient and economic production. In this review, the latest advances on development of stress tolerant S. cerevisiae strains are summarized, with the emphasis on the impact of cell flocculation and zinc addition. It was found that cell flocculation affected ethanol tolerance and acetic acid tolerance of S. cerevisiae, and addition of zinc to a suitable level improved stress tolerance of yeast cells to ethanol, high temperature and acetic acid. Further studies on the underlying mechanisms by which cell flocculation and zinc status affect stress tolerance will not only enrich our knowledge on stress response and tolerance mechanisms of S. cerevisiae, but also provide novel metabolic engineering strategies to develop robust yeast strains for biofuels production.
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Cheng
authors: C
authors: Cheng
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Mingming
authors: M
authors: Zhang
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Chuang
authors: C
authors: Xue
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China; State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors: Fengwu
authors: F
authors: Bai
authors: State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: xqzhao@sjtu.edu.cn.
authors: Xinqing
authors: X
authors: Zhao
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000220:Adaptation, Biological / Q000235:genetics; D056804:Biofuels; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000502:physiology; D005427:Flocculation; D060847:Metabolic Engineering / Q000379:methods / Q000639:trends*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D015032:Zinc / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D056804:Biofuels; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; D015032:Zinc; D019342:Acetic Acid
keywords: Biofuels production; Cell flocculation; Metabolic engineering; Saccharomyces cerevisiae; Yeast stress tolerance; Zinc supplementation