title: Core-Shell Nanorod Columnar Array Combined with Gold Nanoplate-Nanosphere Assemblies Enable Powerful In Situ SERS Detection of Bacteria.
abstract: Development of a label-free ultrasensitive nanosensor for detection of bacteria is presented. Sensitive assay for Gram-positive bacteria was achieved via electrostatic attraction-guided plasmonic bifacial superstructure/bacteria/columnar array assembled in one step. Dynamic optical hotspots were formed in the hybridized nanoassembly under wet-dry critical state amplifying efficiently the weak vibrational modes of three representative food-borne Gram-positive bacteria, that is, Staphylococcus xylosus, Listeria monocytogenes, and Enterococcus faecium. These three bacteria with highly analogous Raman spectra can be effectively differentiated through droplet wet-dry critical SERS approach combined with 3D PCA statistical analysis so that highly sensitive discrimination of bacterial species and samples containing mixtures of bacteria can be achieved.
authors: Institute of Intelligent Machines, HeFei Institutes of Physical Science, Chinese Academy of Sciences , Hefei, Anhui 230031, People's Republic of China.
authors: Li
authors: L
authors: Qiu
authors: Institute of Health Sciences and School of Life Science, AnHui University , Hefei, Anhui 230601, People's Republic of China.
authors: WeiQiang
authors: W
authors: Wang
authors: Institute of Intelligent Machines, HeFei Institutes of Physical Science, Chinese Academy of Sciences , Hefei, Anhui 230031, People's Republic of China.
authors: AiWen
authors: A
authors: Zhang
authors: Institute of Health Sciences and School of Life Science, AnHui University , Hefei, Anhui 230601, People's Republic of China.
authors: NanNan
authors: N
authors: Zhang
authors: Department of Physics, Nanoscience Center, University of Jyväskylä , P.O. Box 35, FI-40014 Jyväskylä, Finland.
authors: Tibebe
authors: T
authors: Lemma
authors: Institute of Health Sciences and School of Life Science, AnHui University , Hefei, Anhui 230601, People's Republic of China.
authors: HongHua
authors: H
authors: Ge
authors: Department of Physics, Nanoscience Center, University of Jyväskylä , P.O. Box 35, FI-40014 Jyväskylä, Finland.
authors: J Jussi
authors: JJ
authors: Toppari
authors: BioMediTech, University of Tampere and Fimlab Laboratories , FI-33520 Tampere, Finland.
authors: Vesa P
authors: VP
authors: Hytönen
authors: Institute of Intelligent Machines, HeFei Institutes of Physical Science, Chinese Academy of Sciences , Hefei, Anhui 230031, People's Republic of China.
authors: Jin
authors: J
authors: Wang
mesh_terms: D006046:Gold; D008089:Listeria monocytogenes; D054118:Nanospheres; D043942:Nanotubes; D013059:Spectrum Analysis, Raman
chemical_list: D006046:Gold
keywords: 3D PCA; Gram-positive bacteria; SERS; nanoarray; superstructure