title: Biosensing applications of titanium dioxide coated graphene modified disposable electrodes.
abstract: In the present work, preparation of titanium dioxide coated graphene (TiO2/graphene) and the use of this nanocomposite modified electrode for electrochemical biosensing applications were detailed. The nanocomposite was prepared with radio frequency (rf) rotating plasma method which serves homogeneous distribution of TiO2 onto graphene. TiO2/graphene was characterized with scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD) analysis. Then, this nanocomposite was dissolved in phosphate buffer solution (pH 7.4) and modified onto disposable pencil graphite electrode (PGE) by dip coating for the investigation of the biosensing properties of the prepared electrode. TiO2/graphene modified PGE was characterized with SEM, EDS and cyclic voltammetry (CV). The sensor properties of the obtained surface were examined for DNA and DNA-drug interaction. The detection limit was calculated as 1.25mgL(-1) (n=3) for double-stranded DNA (dsDNA). RSD% was calculated as 2.4% for three successive determinations at 5mgL(-1) dsDNA concentration. Enhanced results were obtained compared to the ones obtained with graphene and unmodified (bare) electrodes.
authors: Ordu University, Faculty of Arts and Sciences, Department of Chemistry, Ordu, 52200 Turkey. Electronic address: kuralay.filiz@gmail.com.
authors: Filiz
authors: F
authors: Kuralay
authors: Ordu University, Faculty of Arts and Sciences, Department of Chemistry, Ordu, 52200 Turkey.
authors: Selma
authors: S
authors: Tunç
authors: Suleyman Demirel University, Faculty of Arts and Sciences, Department of Chemistry, Isparta, 32260 Turkey.
authors: Ferhat
authors: F
authors: Bozduman
authors: Suleyman Demirel University, Faculty of Arts and Sciences, Department of Chemistry, Isparta, 32260 Turkey.
authors: Lutfi
authors: L
authors: Oksuz
authors: Suleyman Demirel University, Faculty of Arts and Sciences, Department of Physics, Isparta, 32260 Turkey.
authors: Aysegul Uygun
authors: AU
authors: Oksuz
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D061925:Immobilized Nucleic Acids / Q000737:chemistry*; D016685:Mitomycin / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D061925:Immobilized Nucleic Acids; C009495:titanium dioxide; D016685:Mitomycin; D006108:Graphite; D004247:DNA; D014025:Titanium
keywords: DNA; DNA-drug interaction; Electrochemistry; Rotating plasma; TiO(2)/graphene nanocomposite; TiO(2)/graphene nanocomposite modified electrode