title: Presynaptic DLG regulates synaptic function through the localization of voltage-activated Ca(2+) Channels.
abstract: The DLG-MAGUK subfamily of proteins plays a role on the recycling and clustering of glutamate receptors (GLUR) at the postsynaptic density. discs-large1 (dlg) is the only DLG-MAGUK gene in Drosophila and originates two main products, DLGA and DLGS97 which differ by the presence of an L27 domain. Combining electrophysiology, immunostaining and genetic manipulation at the pre and postsynaptic compartments we study the DLG contribution to the basal synaptic-function at the Drosophila larval neuromuscular junction. Our results reveal a specific function of DLGS97 in the regulation of the size of GLUR fields and their subunit composition. Strikingly the absence of any of DLG proteins at the presynaptic terminal disrupts the clustering and localization of the calcium channel DmCa1A subunit (Cacophony), decreases the action potential-evoked release probability and alters short-term plasticity. Our results show for the first time a crucial role of DLG proteins in the presynaptic function in vivo.
authors: Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
authors: César
authors: C
authors: Astorga
authors: Program of Physiology and Biophysics, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago, Chile.|Neuroscience Department, School of Medicine, Universidad Central del Caribe, Bayamon, 00956, PR, USA.
authors: Ramón A
authors: RA
authors: Jorquera
authors: Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
authors: Mauricio
authors: M
authors: Ramírez
authors: Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
authors: Andrés
authors: A
authors: Kohler
authors: Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
authors: Estefanía
authors: E
authors: López
authors: Department of Biology, Faculty of Science, Universidad de Chile, Santiago, Chile.
authors: Ricardo
authors: R
authors: Delgado
authors: Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
authors: Alex
authors: A
authors: Córdova
authors: Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile.|Program of Human Genetics, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
authors: Patricio
authors: P
authors: Olguín
authors: Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile.|Program of Physiology and Biophysics, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
authors: Jimena
authors: J
authors: Sierralta
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D013569:Synapses / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D029721:Drosophila Proteins; D025521:Tumor Suppressor Proteins; C472405:cac protein, Drosophila; C070350:dlg1 protein, Drosophila