title: Reno-protective effects of propolis on gentamicin-induced acute renal toxicity in swiss albino mice.
abstract: BACKGROUND
  Kidney is a vital organ which plays an important and irreplaceable role in detoxification and removal of xenobiotics. And therefore is vulnerable to develop various forms of injuries. Hence, making it immensely important to search for natural reno-protective compounds.
  
  
  OBJECTIVE
  This study therefore, aims to evaluate the reno-protective properties of propolis against gentamicin induced renal toxicity in mice.
  
  
  METHODS
  Three groups of 10 male mice each were used for this study. First group served as control, the second group (Gm group) was administered orally 80mg/kg body weight gentamicin for 7 days, and the third group (GmP group) was administered same dose of gentamicin with propolis (500mg/kg body weight) for 7 days. Various parameters were used to study the renal toxicity.
  
  
  RESULTS
  Gentamicin caused significant renal damage as evident by the rise in BUN levels, diminished glomeruli hypocellularity, moderately dilated tubules, and mild loss of brush border, severe infiltration, extensive tubular degeneration and presence of tubular cast. Histochemistry results show presence of collagen and reticular fibres. Immunohistochemical reactions show kidney injury (Kim-1 gene-expression), oxidative stress (MDA gene-expression), and an increase in apoptosis (caspase-3 gene-expression). Co-administration of propolis with gentamicin showed significant decrease in BUN levels, appearance of healthy glomeruli with normal cellularity, reduction of tubular injury, decrease of collagen and reticular fibres deposition, reduction of apoptosis, kidney injury and oxidative stress.
  
  
  CONCLUSIONS
  Results presented in this study clearly show the reno-protective role of propolis against gentamicin-induced toxicity on mice kidney.
authors: King Saud University, College of Science, Department of Zoology, Riyadh, Saudi Arabia. Electronic address: badr.zool.ksu@gmail.com.
authors: Badr Abdullah
authors: BA
authors: Aldahmash
authors: Ain Shams University, College of Girls for Science, Arts and Education, Department of Zoology, Cairo, Egypt.
authors: Doaa Mohamed
authors: DM
authors: El-Nagar
authors: King Saud University, College of Science, Department of Zoology, Riyadh, Saudi Arabia.
authors: Khalid Elfakki
authors: KE
authors: Ibrahim
mesh_terms: D000818:Animals; D005839:Gentamicins / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology; D008297:Male; D051379:Mice; D018384:Oxidative Stress; D011429:Propolis / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051437:Renal Insufficiency / Q000139:chemically induced / Q000188:drug therapy*
chemical_list: D005839:Gentamicins; D020011:Protective Agents; D011429:Propolis
keywords: Apoptosis; Estrés oxidativo; Gentamicin; Gentamicina; Kidney; Oxidative stress; Propolis; Propóleo; Riñón