title: Effects of donor cells' sex on nuclear transfer efficiency and telomere lengths of cloned goats.
abstract: The aim of this study was to investigate the effects of donor cells' sex on nuclear transfer efficiency and telomere length of cloned goats from adult skin fibroblast cells. The telomere length of somatic cell cloned goats and their offspring was determined by measuring their mean terminal restriction fragment (TRF) length. The result showed that (i) reconstructed embryos with fibroblast cells from males Boer goats obtained significantly higher kids rate and rate of live kids than those of female embryos and (ii) the telomere lengths of four female cloned goats were shorter compared to their donor cells, but five male cloned goats had the same telomere length with their donor cells, mainly due to great variation existed among them. The offspring from female cloned goats had the same telomere length with their age-matched counterparts. In conclusion, the donor cells' sex had significant effects on nuclear transfer efficiency and telomere lengths of cloned goats.
authors: College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, P. R. China.|Tianjin Institute of Animal Science and Veterinary Medicine, Tianjin, P. R. China.
authors: H-J
authors: HJ
authors: Liu
authors: College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, P. R. China.
authors: H
authors: H
authors: Peng
authors: College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei, P. R. China.
authors: C-C
authors: CC
authors: Hu
authors: College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei, P. R. China.
authors: X-Y
authors: XY
authors: Li
authors: Tianjin Institute of Animal Science and Veterinary Medicine, Tianjin, P. R. China.
authors: J-L
authors: JL
authors: Zhang
authors: Tianjin Institute of Animal Science and Veterinary Medicine, Tianjin, P. R. China.
authors: Z
authors: Z
authors: Zheng
authors: College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, P. R. China. zwcfz@163.com.
authors: W-C
authors: WC
authors: Zhang
mesh_terms: D000818:Animals; D019976:Cloning, Organism; D004247:DNA / Q000235:genetics; D004624:Embryo Transfer / Q000662:veterinary; D004622:Embryo, Mammalian; D005260:Female; D006041:Goats / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D017422:Sequence Analysis, DNA / Q000379:methods; D016615:Telomere / Q000235:genetics
chemical_list: D004247:DNA