title: Cloning identification and functional analysis of human IL-17A promoter.
abstract: OBJECTIVE
  To conduct the cloning identification and characterization of the sequence of human IL-17A promoter so as to analyze the regulatory mechanism of the gene expression of IL-17.
  
  
  METHODS
  First of all, the potential promoter region of IL-17A was found by means of the bioinformatics methods. Then, it was cloned into the reporter vector with PCR technique. Finally, the activity of the test promoter was determined by dual luciferase reporter system.
  
  
  RESULTS
  Two transcriptional start points of the upper region, 600 bp and 1000 bp, of IL-17A were obtained by PCR clone and proved to have certain activities by dual luciferase reporter system. Also, they could be activated by IL-17A activator STAT3, which could start the expression of the reported gene.
  
  
  CONCLUSIONS
  Clone established the regulatory region of human IL-17A promoter, which provided bases to the subsequent function research.
authors: School of Life Science, Institute of Neurobiology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui, 233030, China.
authors: Ming-Jie
authors: MJ
authors: Hu
authors: School of Life Science, Institute of Neurobiology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui, 233030, China.
authors: Shou-Wei
authors: SW
authors: Wu
authors: School of Life Science, Institute of Neurobiology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui, 233030, China.
authors: Mei-Li
authors: ML
authors: Wei
authors: School of Life Science, Institute of Neurobiology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui, 233030, China.
authors: Jun
authors: J
authors: Xi
authors: School of Life Science, Institute of Neurobiology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui, 233030, China.
authors: Lu
authors: L
authors: Wang
authors: School of Life Science, Institute of Neurobiology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui, 233030, China.
authors: Yu-Ze
authors: YZ
authors: Han
authors: School of Life Science, Institute of Neurobiology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui, 233030, China.
authors: Bi-Kui
authors: BK
authors: Tang
authors: School of Life Science, Institute of Neurobiology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui, 233030, China.
authors: Qian
authors: Q
authors: Fang
authors: Department of Neurology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, 233003, China. Electronic address: xuli7292@163.com.
authors: Li
authors: L
authors: Xu
keywords: Clone; Functional analysis; IL-17A; Promoter