title: Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology.
abstract: The delayed reporting of antimicrobial susceptibility testing remains a limiting factor in clinical decision-making in the treatment of bacterial infection. This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial susceptibility. Three isolates each (two clinical isolates and one reference strain) of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were tested in triplicate using two commercial antimicrobial testing systems, the Vitek2 and the MicroScan MIC panel, to challenge the BacterioScan FLLS. The BacterioScan FLLS showed a high degree of categorical concordance with the commercial methods. Pairwise comparison with each commercial system serving as a reference standard showed 88.9% agreement with MicroScan (two minor errors) and 72.2% agreement with Vitek (five minor errors). FLLS using the BacterioScan system shows promise as a novel method for the rapid and accurate determination of antimicrobial susceptibility.
authors: St. Jude Children's Research Hospital, Department of Pathology, Memphis, Tennessee, USA randall.hayden@stjude.org.
authors: Randall T
authors: RT
authors: Hayden
authors: Pathology Residency Program, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA.
authors: Lani K
authors: LK
authors: Clinton
authors: St. Jude Children's Research Hospital, Department of Pathology, Memphis, Tennessee, USA.
authors: Carolyn
authors: C
authors: Hewitt
authors: Diagnostic Laboratory Services, Inc. (The Queen's Medical Center), Aiea, Hawaii, USA.
authors: Terri
authors: T
authors: Koyamatsu
authors: St. Jude Children's Research Hospital, Department of Biostatisics, Memphis, Tennessee, USA.
authors: Yilun
authors: Y
authors: Sun
authors: St. Jude Children's Research Hospital, Department of Pathology, Memphis, Tennessee, USA.
authors: Ginger
authors: G
authors: Jamison
authors: St. Jude Children's Research Hospital, Department of Pathology, Memphis, Tennessee, USA.
authors: Rosalie
authors: R
authors: Perkins
authors: St. Jude Children's Research Hospital, Department of Biostatisics, Memphis, Tennessee, USA.
authors: Li
authors: L
authors: Tang
authors: St. Jude Children's Research Hospital, Department of Biostatisics, Memphis, Tennessee, USA.
authors: Stanley
authors: S
authors: Pounds
authors: Diagnostic Laboratory Services, Inc. (The Queen's Medical Center), Aiea, Hawaii, USA.|Department of Pathology and Department of Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA.
authors: Matthew J
authors: MJ
authors: Bankowski
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000067493:Dynamic Light Scattering / Q000379:methods*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D007834:Lasers; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010865:Pilot Projects; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents