title: Molecular cloning and expression analysis of NOD-like receptor 5 in Japanese flounder (Paralichthys olivaceus) after injection with two different formalin-killed pathogenic bacteria and poly (I:C).
abstract: NOD-like receptors (NLRs) are members of pattern recognition receptors (PRRs) recognized intracellular pathogens. Here, we identified a type of NLR with a CARD domain (NLRC5) in Japanese flounder, Paralichthys olivaceus (JfNLRC5). The coding sequence JfNLRC5 is 5529 bp long and encodes a protein of 1842 deduced amino acid residues. JfNLRC5 transcripts were highly detected in gills, intestine and spleen of healthy fish. In Japanese flounder stimulated with poly (I:C), JfNLRC5 was significantly up-regulated after 24 h at 15 °C and after 3 h at 25 °C. Expression of JfNLRC5 was up-regulated by formalin-killed Edwardsiella tarda but not by formalin-killed Streptococcus iniae. These findings suggest that JfNLRC5 is involved in fish immune response against viral and Gram-negative bacterial infections.
authors: Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
authors: Kittipong
authors: K
authors: Thanasaksiri
authors: Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
authors: Ikuo
authors: I
authors: Hirono
authors: Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan. Electronic address: h-kondo@kaiyodai.ac.jp.
authors: Hidehiro
authors: H
authors: Kondo
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001424:Bacterial Infections / Q000276:immunology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D020609:Edwardsiella tarda / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005432:Flounder / Q000276:immunology*; D005557:Formaldehyde / Q000378:metabolism; D005786:Gene Expression Regulation; D005880:Gills / Q000276:immunology* / Q000382:microbiology; D007113:Immunity, Innate; D000070576:NLR Proteins / Q000235:genetics / Q000378:metabolism*; D011070:Poly I-C / Q000276:immunology; D000070058:Streptococcus iniae / Q000276:immunology*; D059467:Transcriptome; D014777:Virus Diseases / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D029941:Fish Proteins; D000070576:NLR Proteins; D005557:Formaldehyde; D011070:Poly I-C
keywords: Edwardsiella tarda; Japanese flounder; NLRC5; Poly (I:C); Streptococcus iniae