title: Effect of Asiaticoside, Collagenase, and Alpha-chymotrypsin on Wound Healing in Rabbits.
abstract: Wound dressing materials such as asiaticoside, collagenase, and alpha-chymotrypsin are often used for effective wound healing activity.
  
  
  OBJECTIVE
  In this study, the effects of asiaticoside, collagenase, and alpha-chymotrypsin were studied in rabbit models with open wounds with tissue loss and with full-thickness flank excisions for a period of 21 days.
  
  
  METHODS
  Three groups of 4 rabbits were examined during trial periods of 7, 14, and 21 days. Four circular wounds measuring 1.5 cm in diameter were made on the dorsal sides of the animals: 2 on the right and 2 the left. Asiaticoside, collagenase, and alpha-chymotrypsin were applied to wounds daily for a period of 7, 14, and 21 days, while 1 gauzed wound served as the control. All biopsy specimens were histopathologically evaluated for recovery. On day 7, microscopic review showed no differences in wound healing between groups.
  
  
  RESULTS
  By day 14, alpha-chymotrypsin showed the quickest reepithelialization (P < 0.05); and by day 21 asiaticoside and collagenase (P < 0.01) showed effective recovery, due to the completion of wound healing for all animals in both groups.
  
  
  CONCLUSIONS
  Alpha-chymotrypsin is more effective than the other 2 groups for only 14 days. The effectiveness of asiaticoside and collagenase displayed a more rapid improvement in comparison to alpha-chymotrypsin for healing open wounds with tissue loss for a period of 21 days.
authors: Faculty of Veterinary Medicine, Department of Pathology, University of Selcuk, Konya, Turkey.
authors: Ozgur
authors: O
authors: Ozdemir
authors: Faculty of Veterinary Medicine, Department of Pathology, University of Selcuk, Konya, Turkey.
authors: Kadircan
authors: K
authors: Ozkan
authors: Faculty of Veterinary Medicine, Department of Pathology, University of Selcuk, Konya, Turkey.
authors: Fatih
authors: F
authors: Hatipoglu
authors: Faculty of Veterinary Medicine, Department of Pathology, University of Selcuk, Konya, Turkey.
authors: Aysen
authors: A
authors: Uyaroglu
authors: Faculty of Veterinary Medicine, Department of Pathology, University of Selcuk, Konya, Turkey.
authors: Mustafa
authors: M
authors: Arican
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001458:Bandages; D002918:Chymotrypsin / Q000494:pharmacology*; D017364:Collagenases / Q000494:pharmacology*; D004195:Disease Models, Animal; D011817:Rabbits; D014315:Triterpenes / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000188:drug therapy / Q000473:pathology*; D014947:Wounds and Injuries / Q000473:pathology*
chemical_list: D000891:Anti-Infective Agents, Local; D014315:Triterpenes; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; D017364:Collagenases; C004446:asiaticoside