title: Optical coherence tomography angiography of stimulus evoked hemodynamic responses in individual retinal layers.
abstract: Blood flow changes are highly related to neural activities in the retina. It has been reported that neural activity increases when flickering light stimulation of the retina is used. It is known that blood flow changes with flickering light stimulation can be altered in patients with vascular disease and that measurement of flicker-induced vasodilatation is an easily applied tool for monitoring functional microvascular alterations. However, details of distortions in retinal neurovascular coupling associated with major eye diseases are not well understood due to the limitation of existing techniques. In this study, flickering light stimulation was applied to mouse retinas to investigate stimulus evoked hemodynamic responses in individual retinal layers. A spectral domain optical coherence tomography (OCT) angiography imaging system was developed to provide dynamic mapping of hemodynamic responses in the ganglion cell layer, inner plexiform layer, outer plexiform layer and choroid layer before, during and after flickering light stimulation. Experimental results showed hemodynamic responses with different magnitudes and time courses in individual retinal layers. We anticipate that the dynamic OCT angiography of stimulus evoked hemodynamic responses can greatly foster the study of neurovascular coupling mechanisms in the retina, promising new biomarkers for retinal disease detection and diagnosis.
authors: Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA.
authors: Taeyoon
authors: T
authors: Son
authors: Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA.
authors: Benquan
authors: B
authors: Wang
authors: Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA.
authors: Damber
authors: D
authors: Thapa
authors: Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA.
authors: Yiming
authors: Y
authors: Lu
authors: Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA.
authors: Yanjun
authors: Y
authors: Chen
authors: Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors: Dingcai
authors: D
authors: Cao
authors: Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA; Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors: Xincheng
authors: X
authors: Yao
keywords: (170.2655) Functional monitoring and imaging; (170.4460) Ophthalmic optics and devices; (170.4470) Ophthalmology; (170.4500) Optical coherence tomography; (330.4300) Vision system - noninvasive assessment