title: Functional regeneration of the transected recurrent laryngeal nerve using a collagen scaffold loaded with laminin and laminin-binding BDNF and GDNF.
abstract: Recurrent laryngeal nerve (RLN) injury remains a challenge due to the lack of effective treatments. In this study, we established a new drug delivery system consisting of a tube of Heal-All Oral Cavity Repair Membrane loaded with laminin and neurotrophic factors and tested its ability to promote functional recovery following RLN injury. We created recombinant fusion proteins consisting of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) fused to laminin-binding domains (LBDs) in order to prevent neurotrophin diffusion. LBD-BDNF, LBD-GDNF, and laminin were injected into a collagen tube that was fitted to the ends of the transected RLN in rats. Functional recovery was assessed 4, 8, and 12 weeks after injury. Although vocal fold movement was not restored until 12 weeks after injury, animals treated with the collagen tube loaded with laminin, LBD-BDNF and LBD-GDNF showed improved recovery in vocalisation, arytenoid cartilage angles, compound muscle action potentials and regenerated fibre area compared to animals treated by autologous nerve grafting (p < 0.05). These results demonstrate the drug delivery system induced nerve regeneration following RLN transection that was superior to that induced by autologus nerve grafting. It may have potential applications in nerve regeneration of RLN transection injury.
authors: Department of Otolaryngology, Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, P.R. China.
authors: Baoxin
authors: B
authors: Wang
authors: Department of Orthopedics, Shanghai Fengxian District Central Hospital, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai 201499, P.R. China.
authors: Junjie
authors: J
authors: Yuan
authors: Department of Otolaryngology, Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, P.R. China.
authors: Xinwei
authors: X
authors: Chen
authors: School of Economics and Finance, Shanghai International Studies University, Shanghai 200083, P.R. China.
authors: Jiafeng
authors: J
authors: Xu
authors: Department of Otolaryngology, Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, P.R. China.
authors: Yu
authors: Y
authors: Li
authors: Department of Otolaryngology, Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, P.R. China.
authors: Pin
authors: P
authors: Dong
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D007797:Laminin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D007823:Laryngeal Nerves / Q000502:physiology*; D061222:Lingual Nerve Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007797:Laminin; D011993:Recombinant Fusion Proteins; D003094:Collagen