title: Effects of silver nanoparticles in combination with antibiotics on the resistant bacteria Acinetobacter baumannii.
abstract: Acinetobacter baumannii resistance to carbapenem antibiotics is a serious clinical challenge. As a newly developed technology, silver nanoparticles (AgNPs) show some excellent characteristics compared to older treatments, and are a candidate for combating A. baumannii infection. However, its mechanism of action remains unclear. In this study, we combined AgNPs with antibiotics to treat carbapenem-resistant A. baumannii (aba1604). Our results showed that single AgNPs completely inhibited A. baumannii growth at 2.5 μg/mL. AgNP treatment also showed synergistic effects with the antibiotics polymixin B and rifampicin, and an additive effect with tigecyline. In vivo, we found that AgNPs-antibiotic combinations led to better survival ratios in A. baumannii-infected mouse peritonitis models than that by single drug treatment. Finally, we employed different antisense RNA-targeted Escherichia coli strains to elucidate the synergistic mechanism involved in bacterial responses to AgNPs and antibiotics.
authors: School of Life Science and Technology, China Pharmaceutical University, Nanjing; Shanghai Laiyi Center for Biopharmaceutical R&D.
authors: Guoqing
authors: G
authors: Wan
authors: Shanghai Laiyi Center for Biopharmaceutical R&D; School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors: Lingao
authors: L
authors: Ruan
authors: Shanghai Laiyi Center for Biopharmaceutical R&D; School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors: Yu
authors: Y
authors: Yin
authors: Shanghai Laiyi Center for Biopharmaceutical R&D; School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors: Tian
authors: T
authors: Yang
authors: Shanghai Laiyi Center for Biopharmaceutical R&D.
authors: Mei
authors: M
authors: Ge
authors: School of Life Science and Technology, China Pharmaceutical University, Nanjing; Department of Integrative Biology & Pharmacology, The University of Texas Health Science Center, Houston, TX, USA.
authors: Xiaodong
authors: X
authors: Cheng
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology; D004357:Drug Synergism; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry; D008810:Mice, Inbred C57BL; D008826:Microbial Sensitivity Tests; D010538:Peritonitis / Q000188:drug therapy / Q000382:microbiology; D012293:Rifampin / Q000494:pharmacology; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D018440:beta-Lactam Resistance / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D012834:Silver; D012293:Rifampin
keywords: Acinetobacter baumannii; AgNPs; antibiotic combination; antisense RNA; synergistic