title: Combined Study of Titanium Dioxide Nanoparticle Transport and Toxicity on Microbial Nitrifying Communities under Single and Repeated Exposures in Soil Columns.
abstract: Soils are exposed to nanoparticles (NPs) as a result of their increasing use in many commercial products. Adverse effects of NPs on soil microorganisms have been reported in several ecotoxicological studies using microcosms. Although repeated exposures are more likely to occur in soils, most of these previous studies were performed as a single exposure to NPs. Contrary to single contamination, the study of multiple NP contaminations in soils requires the use of specialized setups. Using a soil column experiment, we compared the influence of single and repeated exposures (one, two, or three exposures that resulted in the same final concentration applied) on the transport of titanium dioxide (TiO2) NPs through soil and the effect of these different exposure scenarios on the abundance and activity of soil nitrifying microbial communities after a 2 month incubation. The transport of TiO2NPs was very limited under both single and repeated exposures and was highest for the lowest concentration injected during the first application. Significant decreases in nitrification activity and ammonia-oxidizing archaea and bacteria populations were observed only for the repeated exposure scenario (three TiO2NP contaminations). These results suggest that, under repeated exposures, the transport of TiO2NPs to deep soil layers and groundwater is limited and that a chronic contamination is more harmful for the soil microbiological functioning than a single exposure.
authors: Université de Lyon , F-69002 Lyon, France.|Université Claude Bernard Lyon 1 , F-69100 Villeurbanne, France.|Microbial Ecology Lab, UMR 5557, Centre National de la Recherche Scientifique (CNRS), Université Lyon 1 , F-69622 Villeurbanne, France.|Laboratoire d'étude des Transferts en Hydrologie et Environnement (LTHE), UMR 5564, Université Grenoble Alpes , F-38000 Grenoble, France.
authors: Marie
authors: M
authors: Simonin
authors: Laboratoire d'étude des Transferts en Hydrologie et Environnement (LTHE), UMR 5564, Université Grenoble Alpes , F-38000 Grenoble, France.|Laboratoire d'étude des Transferts en Hydrologie et Environnement (LTHE), Centre National de la Recherche Scientifique (CNRS) , F-38000 Grenoble, France.
authors: Jean M F
authors: JM
authors: Martins
authors: Laboratoire d'étude des Transferts en Hydrologie et Environnement (LTHE), UMR 5564, Université Grenoble Alpes , F-38000 Grenoble, France.|Laboratoire d'étude des Transferts en Hydrologie et Environnement (LTHE), Institut de Recherche pour le Développement (IRD) , F-38000 Grenoble, France.
authors: Gaëlle
authors: G
authors: Uzu
authors: Laboratoire d'étude des Transferts en Hydrologie et Environnement (LTHE), UMR 5564, Université Grenoble Alpes , F-38000 Grenoble, France.|Laboratoire d'étude des Transferts en Hydrologie et Environnement (LTHE), Centre National de la Recherche Scientifique (CNRS) , F-38000 Grenoble, France.
authors: Erwann
authors: E
authors: Vince
authors: Université de Lyon , F-69002 Lyon, France.|Université Claude Bernard Lyon 1 , F-69100 Villeurbanne, France.|Microbial Ecology Lab, UMR 5557, Centre National de la Recherche Scientifique (CNRS), Université Lyon 1 , F-69622 Villeurbanne, France.
authors: Agnès
authors: A
authors: Richaume
mesh_terms: D001105:Archaea; D053758:Nanoparticles / Q000633:toxicity; D058465:Nitrification; D012987:Soil; D012988:Soil Microbiology; D014025:Titanium / Q000633:toxicity
chemical_list: D012987:Soil; D014025:Titanium