title: Comprehensive behavioral study and proteomic analyses of CRMP2-deficient mice.
abstract: Collapsin response mediator protein 2 (CRMP2) plays a key role in axon guidance, dendritic morphogenesis and cell polarization. CRMP2 is implicated in various neurological and psychiatric disorders. However, in vivo functions of CRMP2 remain unknown. We generated CRMP2 gene-deficient (crmp2-/-) mice and examined their behavioral phenotypes. During 24-h home cage monitoring, the activity level during the dark phase of crmp2-/-mice was significantly higher than that of wild-type (WT) mice. Moreover, the time during the open arm of an elevated plus maze was longer for crmp2-/-mice than for WT mice. The duration of social interaction was shorter for crmp2-/-mice than for WT mice. Crmp2-/-mice also showed mild impaired contextual learning. We then examined the methamphetamine-induced behavioral change of crmp2-/-mice. Crmp2-/-mice showed increased methamphetamine-induced ambulatory activity and serotonin release. Crmp2-/-mice also showed altered expression of proteins involved in GABAergic synapse, glutamatergic synapse and neurotrophin signaling pathways. In addition, SNAP25, RAB18, FABP5, ARF5 and LDHA, which are related genes to schizophrenia and methamphetamine sensitization, are also decreased in crmp2-/-mice. Our study implies that dysregulation of CRMP2 may be involved in pathophysiology of neuropsychiatric disorders.
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.|Department of Neurology and Stroke Medicine, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.
authors: Haruko
authors: H
authors: Nakamura
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.|JSPS Postdoctoral Fellowship for Research Abroad, Tokyo, 102-0083, Japan.
authors: Naoya
authors: N
authors: Yamashita
authors: Advanced Medical Research Center, Yokohama City University, Yokohama, 236-0004, Japan.
authors: Ayuko
authors: A
authors: Kimura
authors: Advanced Medical Research Center, Yokohama City University, Yokohama, 236-0004, Japan.
authors: Yayoi
authors: Y
authors: Kimura
authors: Advanced Medical Research Center, Yokohama City University, Yokohama, 236-0004, Japan.
authors: Hisashi
authors: H
authors: Hirano
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.
authors: Hiroko
authors: H
authors: Makihara
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.|Department of Neurology and Stroke Medicine, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.
authors: Yuko
authors: Y
authors: Kawamoto
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.
authors: Aoi
authors: A
authors: Jitsuki-Takahashi
authors: Department of Anesthesiology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.
authors: Kumiko
authors: K
authors: Yonezaki
authors: Department of Anesthesiology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.|Laboratory of Psychology, Jichi Medical University, Shimotsuke, 329-0498, Japan.
authors: Kenkichi
authors: K
authors: Takase
authors: Department of Anesthesiology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.|Department of Physiology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.
authors: Tomoyuki
authors: T
authors: Miyazaki
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.
authors: Fumio
authors: F
authors: Nakamura
authors: Department of Neurology and Stroke Medicine, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan.
authors: Fumiaki
authors: F
authors: Tanaka
authors: Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan. goshima@med.yokohama-cu.ac.jp.
authors: Yoshio
authors: Y
authors: Goshima
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D004195:Disease Models, Animal; D036341:Intercellular Signaling Peptides and Proteins / Q000172:deficiency / Q000235:genetics* / Q000502:physiology*; D007859:Learning Disabilities / Q000378:metabolism; D008297:Male; D001523:Mental Disorders / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D009422:Nervous System Diseases / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000378:metabolism; D020543:Proteome
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D020543:Proteome; C095114:collapsin response mediator protein-2