title: Neougonin A Inhibits Lipopolysaccharide-Induced Inflammatory Responses via Downregulation of the NF-kB Signaling Pathway in RAW 264.7 Macrophages.
abstract: Neougonin A is a prenylated flavonoid isolated from the whole plants of Helminthostachys zeylanica (Ophioglossaceae), which was usually used as traditional Chinese herbal medicine for the treatment of fever and inflammation. In this study, the pharmacological effects and underlying molecular mechanisms of neougonin A on lipopolysaccharide (LPS)-induced inflammatory responses were investigated. We observed that neougonin A reduced the production of inflammatory mediators (TNFα, PGE2, NO, IL-1β, and IL-6; P < 0.001) and inflammation-related proteins (iNOS and COX-2) induced by LPS in murine macrophage RAW 264.7 cells. Furthermore, Western blot and immunofluorescence analysis indicated that neougonin A could inhibit the phosphorylation of IkBα and block the translocation of NF-kB/p65 into the nucleus even at 1.25 μM (P < 0.05), but have no effect on JNK, ERK1/2, and p38MAPK phosphorylation. It was suggested that the anti-inflammatory actions of neougonin A might be due to the downregulation of TNFα, IL-1β, IL-6, NO, and PGE2via the suppression of NF-kB signal transduction pathway.
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China.
authors: Lang
authors: L
authors: Cao
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China.
authors: Rongtao
authors: R
authors: Li
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China.
authors: Xuanqin
authors: X
authors: Chen
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China.
authors: Yong
authors: Y
authors: Xue
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China. liudankm512@163.com.
authors: Dan
authors: http://orcid.org/0000-0001-5176-9651
authors: D
authors: Liu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D015232:Dinoprostone; D015536:Down-Regulation / Q000187:drug effects*; D005419:Flavonoids / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy*; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors*; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide; D000067996:RAW 264.7 Cells; D015398:Signal Transduction / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C000613627:neougonin A; D009569:Nitric Oxide; D015232:Dinoprostone
keywords: anti-inflammatory; inflammatory mediators; neougonin A; nitric oxide; nuclear factor-kappa B