title: Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation.
abstract: Lipid droplets are organelles found in most mammalian cells, as well as in various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol in which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation. © 2016 by John Wiley & Sons, Inc.
authors: Rutgers, The State University of New Jersey, New Brunswick, New Jersey.
authors: Dawn L
authors: DL
authors: Brasaemle
authors: Washington University School of Medicine, St. Louis, Missouri.
authors: Nathan E
authors: NE
authors: Wolins
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient / Q000379:methods*; D015151:Immunoblotting; D066292:Lipid Droplets / Q000737:chemistry*; D008055:Lipids / Q000302:isolation & purification; D051379:Mice; D011506:Proteins / Q000378:metabolism; D012995:Solubility; D013194:Staining and Labeling
chemical_list: D008055:Lipids; D011506:Proteins
keywords: cell cultures; density-gradient centrifugation; isolation of lipid droplets; mammalian; preparation of lipid droplets by centrifugation