title: Short communication: Follicle superstimulation before ovum pick-up for in vitro embryo production in Holstein cows.
abstract: The objective was to evaluate in vitro embryo production (IVEP) in nonlactating Holstein cows after ovarian superstimulation. Cows were randomly assigned in a crossover design to 1 of 2 groups: control (n=35), which was not synchronized and not treated with hormones before ovum pick-up (OPU), or hormone-treated (n=35), in which wave emergence was synchronized and animals treated with porcine (p)-FSH in the presence of norgestomet before OPU. In the hormone-treated group, all follicles ≥7mm in diameter were aspirated for synchronization of wave emergence and cows received a norgestomet ear implant. After 36h, treatment with p-FSH (6 doses of 40mg each, 12h apart, i.m.) started. Ovum pick-up from follicles >2mm in diameter was performed 44h after the last p-FSH (coasting). Then, IVEP was performed. The total number of cumulus-oocyte complexes recovered (16.0 vs. 20.5±2.2) and number of grades I to III (viable) oocytes (10.7 vs. 12.3±1.6) did not differ between hormone-treated and control groups Additionally, no differences were found in the number of blastocysts per cow per OPU (3.0 vs. 2.6±0.5) or in blastocyst rates (17.1 vs. 12.2±2.4%) between hormone-treated and control, respectively. Thus, in this study, ovarian follicle superstimulation with p-FSH followed by coasting in nonlactating Holstein cows that had synchronization of wave emergence and progestin supplementation did not improve oocyte quality or IVEP compared to no hormonal treatment.
authors: Department of Animal Science, University of São Paulo, Piracicaba, SP 13418-900 Brazil.
authors: Louise H
authors: LH
authors: Oliveira
authors: Department of Animal Production, São Paulo State University, Botucatu, SP 18168-000 Brazil.
authors: Carlos P
authors: CP
authors: Sanches
authors: Alcance Rural, Itapeva, SP 18400-970 Brazil.
authors: Adriano S
authors: AS
authors: Seddon
authors: Central Pioneiros, Carambeí, PR 84145-000 Brazil.
authors: Marcio B
authors: MB
authors: Veras
authors: Central Pioneiros, Carambeí, PR 84145-000 Brazil.
authors: Flávio A
authors: FA
authors: Lima
authors: Department of Animal Science, University of São Paulo, Piracicaba, SP 13418-900 Brazil.
authors: Pedro L J
authors: PLJ
authors: Monteiro
authors: Department of Dairy Science, University of Wisconsin-Madison, Madison 53706.
authors: Milo C
authors: MC
authors: Wiltbank
authors: Department of Animal Science, University of São Paulo, Piracicaba, SP 13418-900 Brazil. Electronic address: robertosartori@usp.br.
authors: Roberto
authors: R
authors: Sartori
mesh_terms: D000818:Animals; D001755:Blastocyst; D002417:Cattle; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D005640:Follicle Stimulating Hormone; D009865:Oocytes; D006080:Ovarian Follicle; D010063:Ovum
chemical_list: D005640:Follicle Stimulating Hormone
keywords: biotechnology; blastocyst; cattle; oocyte