title: Aminopyridinyl-Pseudodeoxycytidine Derivatives Selectively Stabilize Antiparallel Triplex DNA with Multiple CG Inversion Sites.
abstract: The sequence-specific formation of triplex DNA offers a potential basis for genome-targeting technologies. In an antiparallel triplex DNA, the sequence-specificity is established by the formation of specific base triplets (G-GC, A-AT, and T-AT) between a triplex-forming oligonucleotide (TFO) and a duplex DNA. However, there are no natural nucleosides that can selectively recognize the inverted CG and TA base pairs. Therefore, the recognition of the CG and TA inversion sites to form a stable triplex DNA has been a long-standing goal for the triplex-forming technology. We now describe the design and synthesis of pseudo-deoxycytidine (ΨdC) derivatives for selective recognition of the CG base pair to expand the triplex-forming sequence. The aminopyridine-bearing ΨdC derivatives showed high selectivity and affinity toward the CG base pair in all neighboring base contexts. Remarkably, 3-methyl-2-aminopyridinyl-ΨdC ((Me) AP-ΨdC) formed a stable triplex with the promoter sequence of the hTERT gene containing four CG inversion sites, and effectively inhibited its transcription in human cancer cells. Thus, (Me) AP-ΨdC is expected to serve as a new starting point of triplex-forming oligonucleotides for a wide variety of genome-targeting applications.
authors: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors: Hidenori
authors: 0000-0002-2792-9595
authors: H
authors: Okamura
authors: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. taniguch@phar.kyushu-u.ac.jp.
authors: Yosuke
authors: 0000-0002-8985-3629
authors: Y
authors: Taniguchi
authors: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. sasaki@phar.kyushu-u.ac.jp.
authors: Shigeki
authors: 0000-0003-2013-2441
authors: S
authors: Sasaki
mesh_terms: D000631:Aminopyridines / Q000737:chemistry*; D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D003841:Deoxycytidine / Q000138:chemical synthesis / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006860:Hydrogen Bonding; D007700:Kinetics; D008856:Microscopy, Fluorescence; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D019098:Telomerase / Q000235:genetics
chemical_list: D000631:Aminopyridines; C051802:triplex DNA; D003841:Deoxycytidine; D004247:DNA; C509186:TERT protein, human; D019098:Telomerase; D016650:Fluorescein-5-isothiocyanate
keywords: antigenes; hTERT genes; inversion sites; pseudocytidines; triplex DNA