title: Development and validation of a LC-MS/MS method for the quantification of tenofovir and emtricitabine in seminal plasma.
abstract: Accurate and sensitive liquid-chromatography tandem mass spectrometry method for the quantification of tenofovir and emtricitabine in seminal plasma has been developed and full validated. Molecules were separated by high-performance liquid chromatography on an Atlantis T3 C18 column using a gradient of deionized water and methanol, including 0.05% formic acid (250μl/min) and detected by electrospray ionisation/tandem mass spectrometry in positive ion mode. The method was validated over a clinical range of 3.13-1000ng/mL for tenofovir and 6.25-2000ng/mL for emtricitabine. Inter and intra-assay precisions were <9.37% for tenofovir and<10.88% for emtricitabine, and accuracies were between 0.48% and 8.43% for tenofovir, and between 0.64% and 13.87% for emtricitabine. The developed method was successfully applied for analysing tenofovir and emtricitabine concentrations in seminal plasma samples from a clinical study. The use of tandem mass spectrometry can be a suitable method for the analysis of this kind of matrices, providing high sensitivity and specificity to the analysis.
authors: EA 7323, Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France; Unité de Recherche Clinique, Assistance Publique-Hôpitaux de Paris (APHP), Hôpital Tarnier, 75006 Paris, France. Electronic address: 07silvia@gmail.com.
authors: S M
authors: SM
authors: Illamola
authors: EA 7323, Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France; Unité de Recherche Clinique, Assistance Publique-Hôpitaux de Paris (APHP), Hôpital Tarnier, 75006 Paris, France.
authors: E
authors: E
authors: Valade
authors: EA 7323, Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France; Assistance Publique-Hôpitaux de Paris (APHP), Groupe Hospitalier Universitaire (GHU) Ouest, Centre Hospitalier Universitaire (CHU) Cochin, Service de Pharmacologie Clinique, 75014 Paris, France.
authors: D
authors: D
authors: Hirt
authors: Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Assistance Publique-Hôpitaux de Paris (APHP), Groupe Hospitalier Universitaire (GHU) Ouest, Centre Hospitalier Universitaire (CHU) Cochin, Department of Histology, Embryology and Biology of Reproduction, 75679 Paris, France.
authors: E
authors: E
authors: Dulioust
authors: Unité de Recherche Clinique, Assistance Publique-Hôpitaux de Paris (APHP), Hôpital Tarnier, 75006 Paris, France.
authors: Y
authors: Y
authors: Zheng
authors: Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Assistance Publique-Hôpitaux de Paris (APHP), Groupe Hospitalier Universitaire (GHU) Ouest, Centre Hospitalier Universitaire (CHU) Cochin, Department of Histology, Embryology and Biology of Reproduction, 75679 Paris, France.
authors: J P
authors: JP
authors: Wolf
authors: EA 7323, Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France; Unité de Recherche Clinique, Assistance Publique-Hôpitaux de Paris (APHP), Hôpital Tarnier, 75006 Paris, France; Assistance Publique-Hôpitaux de Paris (APHP), Groupe Hospitalier Universitaire (GHU) Ouest, Centre Hospitalier Universitaire (CHU) Cochin, Service de Pharmacologie Clinique, 75014 Paris, France; CIC-0901 Inserm, Cochin-Necker, 75015 Paris, France.
authors: J M
authors: JM
authors: Tréluyer
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D000068679:Emtricitabine / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008297:Male; D012015:Reference Standards; D015203:Reproducibility of Results; D012661:Semen / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000068698:Tenofovir / Q000032:analysis* / Q000737:chemistry
chemical_list: D000068698:Tenofovir; D000068679:Emtricitabine
keywords: Emtricitabine; LC–MS/MS; Seminal plasma; Tenofovir