title: Structural characterization of a novel neutral polysaccharide from Lentinus giganteus and its antitumor activity through inducing apoptosis.
abstract: A novel neutral polysaccharide (LGPS-1), with a molecular weight of 1.547×10(5)Da, was isolated from Lentinus giganteus by precipitation and purification. The monosaccharides included d-mannose (Man), d-glucose (Glc) and d-galactose (Gal) with a molar ratio of 3.0:4.1:7.1. The backbone of LGPS-1 was composed of 1,6-Galp and 1,3,6-Manp whereas the branches were composed of 1,6-Glcp and 1-Glcp. The anticancer efficacy of LGPS-1 was assessed using HepG2 hepatocellular carcinoma cells. The results showed that LGPS-1 inhibited the proliferation of HepG2 cells and also induced the activation of caspase-3, and cleavage of PARP-1. Western blot analysis revealed that LGSP-1 significantly induced a loss of mitochondrial membrane potential (Δym), increased the ratio of Bax/Bcl-2, promoted the release of cytochrome c into cytoplasm as well as inhibited the phosphorylation of Akt in HepG2 cells. These findings suggest that LGPS-1 induced apoptosis in HepG2 cells through intrinsic mitochondrial apoptosis and PI3K/Akt signaling pathways.
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China; Fujian Provincial Key Lab of Quality Science and Processing Technology in Special Starch, Fuzhou, Fujian 350002, PR China.
authors: Yuting
authors: Y
authors: Tian
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
authors: Yingting
authors: Y
authors: Zhao
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
authors: Hongliang
authors: H
authors: Zeng
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
authors: Yaling
authors: Y
authors: Zhang
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China; Fujian Provincial Key Lab of Quality Science and Processing Technology in Special Starch, Fuzhou, Fujian 350002, PR China. Electronic address: zbdfst@hotmail.com.
authors: Baodong
authors: B
authors: Zheng
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002236:Carbohydrate Conformation; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D020074:Lentinula / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009005:Monosaccharides / Q000032:analysis; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000970:Antineoplastic Agents; D062610:Fungal Polysaccharides; D009005:Monosaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D051057:Proto-Oncogene Proteins c-akt
keywords: Antitumor activity; Apoptosis; Lentinus giganteus; Polysaccharide; Structural characterization