title: Characterization of cAMP-phosphodiesterase activity in bovine seminal plasma.
abstract: The second messenger cyclic adenosine monophosphate (cAMP) has a central role in sperm physiology. Extracellular cAMP can be sequentially degraded into 5'AMP and adenosine by ecto-phosphodiesterases (ecto-PDE) and ecto-nucleotidases, a phenomenon called extracellular cAMP-adenosine pathway. As cAMP-adenosine pathway is involved in sperm capacitation, we hypothesize that extracellular PDEs are functionally present in seminal plasma. Exclusively measuring cAMP-PDE activity, total activity in bovine seminal plasma was 10.1 ± 1.5 fmoles/min/μg. Using different family-specific PDE inhibitors, we showed that in seminal plasma, the major cAMP-PDE activity was papaverine sensitive (47.5%). These data support the presence of PDE10 in bovine seminal plasma and was further confirmed by western blot. In epididymal fluid, total cAMP-PDE activity was 48.2 ± 14.8 fmoles/min/μg and we showed that the major cAMP-PDE activity was 3-isobutyl-methylxanthine insensitive and thus ascribed to PDE8 family. PDE10A mRNAs were found in the testis, epididymis, and seminal vesicles. cAMP-PDE activity is present in bovine seminal plasma and epididymal fluid. The results suggest a role for ecto-PDEs present in those fluids in the signaling pathways involved in sperm functions.
authors: Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle, Université Laval, Québec, QC, Canada.|Département des Sciences Animales, Faculté des Sciences de L'agriculture et de L'alimentation, Université Laval, Québec, QC, Canada.
authors: A
authors: A
authors: Bergeron
authors: Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle, Université Laval, Québec, QC, Canada.|Département des Sciences Animales, Faculté des Sciences de L'agriculture et de L'alimentation, Université Laval, Québec, QC, Canada.
authors: J P
authors: JP
authors: Aragon
authors: Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle, Université Laval, Québec, QC, Canada.|Département des Sciences Animales, Faculté des Sciences de L'agriculture et de L'alimentation, Université Laval, Québec, QC, Canada.
authors: C
authors: C
authors: Guillemette
authors: Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle, Université Laval, Québec, QC, Canada.|Département des Sciences Animales, Faculté des Sciences de L'agriculture et de L'alimentation, Université Laval, Québec, QC, Canada.
authors: A
authors: A
authors: Hébert
authors: Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle, Université Laval, Québec, QC, Canada.|Département Obstétrique, Gynécologie et Reproduction, Faculté de Médecine, Université Laval, Québec, QC, Canada.
authors: R
authors: R
authors: Sullivan
authors: Boviteq, Saint-Hyacinthe, QC, Canada.
authors: P
authors: P
authors: Blondin
authors: Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle, Université Laval, Québec, QC, Canada.|Département des Sciences Animales, Faculté des Sciences de L'agriculture et de L'alimentation, Université Laval, Québec, QC, Canada.
authors: F J
authors: FJ
authors: Richard
mesh_terms: D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism*; D008297:Male; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D012661:Semen / Q000187:drug effects / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism
chemical_list: D010726:Phosphodiesterase Inhibitors; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases
keywords: cAMP-phosphodiesterase; seminal plasma; spermatozoa