title: Baicalin Scavenged Reactive Oxygen Species and Protected Human Keratinocytes Against UVB-induced Cytotoxicity.
abstract: Ultraviolet B (UVB), with a wavelength of 280-320 nm, represents one of the most important environmental factors for skin disorders, including sunburn, hyperpigmentation, solar keratosis, solar elastosis and skin cancer. Therefore, protection against excessive UVA-induced damage is useful for prevention of sunburn and other human diseases. Baicalin, a major component of traditional Chinese medicine Scutellaria baicalensis, has been reported to possess antioxidant and cytostatic capacities. In this study, we examined whether baicalin is also capable of protecting human keratinocytes from UVB irradiation. The results showed that baicalin effectively scavenged reactive oxygen species (ROS) elevated within 4 h after UVB radiation and reversed the UVB-suppressed cell viability and UVB-induced apoptosis after 24 h. Our results demonstrated the utility of baicalin to complement the contributions of traditional Chinese medicine in UVB-induced damage to skin and suggested their potential application as pharmaceutical agents in long-term sun-shining injury prevention.
authors: Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Wen-Shin
authors: WS
authors: Chang
authors: Department of Neurosurgery, Taipei Medical University Hospital, Taipei, Taiwan, R.O.C.
authors: En-Yuan
authors: EY
authors: Lin
authors: Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C.
authors: Shih-Wei
authors: SW
authors: Hsu
authors: Department of Ophthalmology, Changhua Christian Hospital, Changhua, Taiwan, R.O.C.
authors: Pei-Shin
authors: PS
authors: Hu
authors: Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C.
authors: Chin-Liang
authors: CL
authors: Chuang
authors: Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C.
authors: Cheng-Hsi
authors: CH
authors: Liao
authors: Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C.
authors: Chun-Kai
authors: CK
authors: Fu
authors: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Chung-Hao
authors: CH
authors: Su
authors: Department of Physiology, China Medical University, Taichung, Taiwan, R.O.C.
authors: Chi-Li
authors: CL
authors: Gong
authors: Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors: Chieh-Lun
authors: CL
authors: Hsiao
authors: Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C. datian@mail.cmuh.org.tw artbau2@gmail.com.
authors: DA-Tian
authors: DT
authors: Bau
authors: Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, R.O.C Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C. datian@mail.cmuh.org.tw artbau2@gmail.com.
authors: Chia-Wen
authors: CW
authors: Tsai
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D005419:Flavonoids / Q000008:administration & dosage*; D006801:Humans; D017495:Hyperpigmentation / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D015603:Keratinocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D007642:Keratosis / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D020011:Protective Agents / Q000008:administration & dosage*; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000528:radiation effects; D012867:Skin / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D012878:Skin Neoplasms / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D013471:Sunburn / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D020011:Protective Agents; D017382:Reactive Oxygen Species; C038044:baicalin
keywords: Baicalin; UVB; keratinocytes; reactive oxygen species; traditional Chinese medicine