title: MiR-139-5p is Increased in the Peripheral Blood of Patients with Prostate Cancer.
abstract: BACKGROUND
  Emerging evidence suggested that microRNAs (miRNAs) play a causal role in cancer tumorigenesis. Aberrant expression of miRNA (miR)-139-5p has been observed in various types of cancers. The present study evaluated the relationship between miR-139-5p expression levels and prostate cancer (PCa), to assess the feasibility of using peripheral blood miR-139-5p as a potential non-invasive biomarker for PCa.
  
  
  METHODS
  Total RNA was extracted from peripheral whole blood samples from 45 PCa patients, 45 benign prostatic hyperplasia (BPH) patients and 50 healthy controls (HC). The expression of miR-139-5p was assessed by reverse transcription quantitative polymerase chain reaction.
  
  
  RESULTS
  MiR-139-5p in peripheral blood was significantly higher in PCa patients than in patients with BPH and HC individuals (P<0.001). Higher miR-139-5p expression was observed to be associated with certain clinicopathological parameters, including PSA>20ng/ml (P<0.05), pathological tumor stage 3/4 (P<0.05) and Gleason score >7 (P<0.01). A receiver operating characteristic (ROC) curve analysis revealed that miR-139-5p distinguished PCa patients from BPH patients [area under the curve (AUC), 0.936; 95% CI, 0.878-0.993; P<0.001].
  
  
  CONCLUSIONS
  Peripheral blood miR-139-5p may be utilized as a potential novel non-invasive biomarker for PCa screening.
authors: Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors: Cheng
authors: C
authors: Pang
authors: Ming
authors: M
authors: Liu
authors: Weiwei
authors: W
authors: Fang
authors: Jun
authors: J
authors: Guo
authors: Zhipeng
authors: Z
authors: Zhang
authors: Pengjie
authors: P
authors: Wu
authors: Yaoguang
authors: Y
authors: Zhang
authors: Jianye
authors: J
authors: Wang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D003937:Diagnosis, Differential; D015870:Gene Expression; D006801:Humans; D007610:Kallikreins / Q000097:blood / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D017430:Prostate-Specific Antigen / Q000097:blood / Q000235:genetics*; D011470:Prostatic Hyperplasia / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D012372:ROC Curve
chemical_list: D014408:Biomarkers, Tumor; C555098:MIRN139 microRNA, human; D035683:MicroRNAs; C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen