title: OPA1 functionally interacts with MIC60 but is dispensable for crista junction formation.
abstract: Remodeling of crista junctions (CJs) is observed in numerous human disorders and during apoptosis. The functional interplay of OPA1 and MIC60, two key players in this context, is unclear. We show that OPA1 modulates cristae morphology but is dispensable for CJ formation. MIC60 is strongly enriched at CJs, whereas OPA1 is distributed evenly across the inner membrane. MIC60 levels are increased in OPA1-/-cells which show increased cellular resistance to apoptosis induction. Endogenous OPA1 and MIC60 show a physical interaction. Overall, we suggest that the regulation of CJ remodeling during apoptosis is mediated via an interplay between OPA1 and MIC60.
authors: Mitochondrial Biology, Buchmann Institute of Molecular Life Sciences, Goethe University Frankfurt, Frankfurt am Main, Germany.
authors: Miguel
authors: M
authors: Barrera
authors: Mitochondrial Biology, Buchmann Institute of Molecular Life Sciences, Goethe University Frankfurt, Frankfurt am Main, Germany.
authors: Sebastian
authors: S
authors: Koob
authors: Mitochondrial Biology, Buchmann Institute of Molecular Life Sciences, Goethe University Frankfurt, Frankfurt am Main, Germany.
authors: Daniel
authors: D
authors: Dikov
authors: Max-Delbrück-Centrum für Molekulare Medizin, Berlin/Buch, Germany.
authors: Frank
authors: F
authors: Vogel
authors: Mitochondrial Biology, Buchmann Institute of Molecular Life Sciences, Goethe University Frankfurt, Frankfurt am Main, Germany. reichert@hhu.de.|Institute of Biochemistry and Molecular Biology I, Medical Faculty, Heinrich-Heine University Düsseldorf, Germany. reichert@hhu.de.
authors: Andreas S
authors: AS
authors: Reichert
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D051336:Mitochondrial Membranes / Q000378:metabolism* / Q000648:ultrastructure; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D024101:Mitochondrial Proteins; D009124:Muscle Proteins; C532603:mitofilin protein, mouse; D020558:GTP Phosphohydrolases; C498809:Opa1 protein, mouse
keywords: MICOS; Mitofilin; apoptosis; crista junction; mitochondria