title: Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy.
abstract: The components for connecting high-performance liquid chromatography (HPLC) with Fourier-transform infrared spectroscopy (FTIR) were investigated to determine estrogen in the water environment, including heating for atomization, solvent removal, sample deposition, drive control, spectrum collection, chip swap, cleaning and drying. Results showed that when the atomization temperature was increased to 388 K, the interference of mobile phase components (methanol, H2O, acetonitrile, and NaH2PO4) were completely removed in the IR measurement of estrogen, with 0.999 of similarity between IR spectra obtained after separation and corresponding to the standard IR spectra. In experiments with varying HPLC injection volumes, high similarity for IR spectra was obtained at 20 ul injection volume at 0.01 mg/L BPA while a useful IR spectrum for 10 ng/L BPA was obtained at 80 ul injection volume. In addition, estrogen concentrations in the natural water samples were calculated semi-quantitatively from the peak intensities of IR spectrum in the mid-infrared region.
authors: Key Laboratory of Drinking Water Science and Technology, Chinese Academy of Sciences, Beijing 100085, China.
authors: Bei
authors: B
authors: Zheng
authors: Key Laboratory of Drinking Water Science and Technology, Chinese Academy of Sciences, Beijing 100085, China.
authors: Wentao
authors: W
authors: Li
authors: Key Laboratory of Drinking Water Science and Technology, Chinese Academy of Sciences, Beijing 100085, China.
authors: Hongyan
authors: H
authors: Li
authors: Northern Engineering Design and Research International Co., Shijiazhuang 050011, China.
authors: Lin
authors: L
authors: Liu
authors: Key Laboratory of Drinking Water Science and Technology, Chinese Academy of Sciences, Beijing 100085, China.
authors: Pei
authors: P
authors: Lei
authors: Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors: Xiaopeng
authors: X
authors: Ge
authors: Key Laboratory of Drinking Water Science and Technology, Chinese Academy of Sciences, Beijing 100085, China.
authors: Zhiyong
authors: Z
authors: Yu
authors: Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors: Yiqi
authors: Y
authors: Zhou
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D004967:Estrogens / Q000737:chemistry / Q000302:isolation & purification*; D057230:Limit of Detection; D045483:Rivers / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004967:Estrogens; D014874:Water Pollutants, Chemical