title: Nanoformulation of curcumin protects HUVEC endothelial cells against ionizing radiation and suppresses their adhesion to monocytes: potential in prevention of radiation-induced atherosclerosis.
abstract: OBJECTIVE
  To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to human THP-1 monocytoid cells.
  
  
  RESULTS
  Co60gamma rays reduced viability, raised the expression of adhesion molecules, ICAM-1, VCAM-1 and E-selectin (mRNA and protein), augmented the adhesion of THP-1 cells to HUVECs, activated NF-κB binding, increased the release of pro-inflammatory cytokines (IL-6, IL-8 and MCP-1) and induced oxidative damage (reduced glutathione declined, while 8-OHdG and TBARS increased). 5 µM DNC significantly inhibited these radiation-induced changes, activated the Nrf-2 pathway, and effectively suppressed THP-1 adhesion to HUVECs, implicating p38 MAPK signaling.
  
  
  CONCLUSIONS
  DNC treatment is a potential preventive method against inflammation and vascular damage from ionizing radiation.
authors: Department of Biotechnology, School of Chemistry, College of Science, University of Tehran, 14155-6455, Tehran, Iran.
authors: Behrooz
authors: B
authors: Soltani
authors: Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Narges
authors: N
authors: Bodaghabadi
authors: Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran.|Department of Biology, Azad University of Damghan, Damghan, Iran.
authors: Gita
authors: G
authors: Mahpour
authors: Department of Biotechnology, School of Chemistry, College of Science, University of Tehran, 14155-6455, Tehran, Iran. nasser.ghaemi@khayam.ut.ac.ir.
authors: Nasser
authors: N
authors: Ghaemi
authors: Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Majid
authors: M
authors: Sadeghizadeh
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D050197:Atherosclerosis / Q000209:etiology* / Q000517:prevention & control*; D002448:Cell Adhesion / Q000187:drug effects* / Q000528:radiation effects*; D003474:Curcumin / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects; D011839:Radiation, Ionizing; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D016207:Cytokines; D017392:Thiobarbituric Acid Reactive Substances; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D005978:Glutathione; D003474:Curcumin
keywords: Adhesion molecules; Curcumin; Human umbilical vein endothelial cells; Ionizing radiation; Monocytes; Nanoformulation