title: Local in vitro delivery of rapamycin from electrospun PEO/PDLLA nanofibers for glioblastoma treatment.
abstract: Rapamycin, a mammalian target of rapamycin inhibitor and anti-proliferative agent, is used to treat glioma and other malignancies, but its effectiveness is limited by the fact that it cannot be delivered in a targeted manner to the site of the tumor. To address this issue, we fabricated a mesh via electrospinning using two biodegradable materials, poly(lactic acid) (PLA) and polyethylene oxide (PEO) as a carrier for rapamycin delivery to the tumor. Nanofiber diameter decreased with increasing PLA concentration in the mixed solution. Scanning electron microscopy analysis revealed the smooth and uniform surface morphology of hybrid fibers. Fourier transform infrared spectroscopy analysis demonstrated that rapamycin was encapsulated in the polymer solution; encapsulation efficiency was high and stable over the range of drug concentrations from 0.5-2wt%. A correlation was observed between sustained release of the drug in vitro and cytotoxicity in cultured glioma cells. These results indicate that the PEO/poly(d,l-lactic acid) nanofiber mesh can be used as a targeted delivery system for rapamycin that can limit side effects and prevent locoregional recurrence following surgical resection of glioma.
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, China.
authors: Benlin
authors: B
authors: Wang
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, China.
authors: Haoyuan
authors: H
authors: Li
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, China.
authors: Qingyu
authors: Q
authors: Yao
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, China.
authors: Yulin
authors: Y
authors: Zhang
authors: Affiliated Hospital of Jining Medical University, Jining 272000, China.
authors: Xiaodong
authors: X
authors: Zhu
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, China.
authors: Tongliang
authors: T
authors: Xia
authors: Brain Science Research Institute, Shandong University, Jinan 250012, China; Department of Biomedicine, University of Bergen, Jonas Lies Vei 91, 5009 Bergen, Norway.
authors: Jian
authors: J
authors: Wang
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, China; Brain Science Research Institute, Shandong University, Jinan 250012, China.
authors: Gang
authors: G
authors: Li
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, China; Brain Science Research Institute, Shandong University, Jinan 250012, China.
authors: Xingang
authors: X
authors: Li
authors: Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, China. Electronic address: ytfishn@126.com.
authors: Shilei
authors: S
authors: Ni
mesh_terms: D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D065546:Drug Liberation; D005909:Glioblastoma / Q000188:drug therapy / Q000378:metabolism; D006801:Humans; D007773:Lactates / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D057139:Nanofibers / Q000008:administration & dosage / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D020123:Sirolimus / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D016896:Treatment Outcome; D014961:X-Ray Diffraction
chemical_list: D007773:Lactates; C058523:polyethylene oxide-polylactic acid block copolymer; D011092:Polyethylene Glycols; D020123:Sirolimus
keywords: Cell viability; Electrospinning; Glioma; Local delivery; Nanofiber; Rapa