title: Intensity-specific effect of physical activity on urinary levels of 8-hydroxydeoxyguanosine in middle-aged Japanese.
abstract: Physical activity (PA) is recommended to both promote and maintain health and prevent cancer by improving the body's DNA repair system, which is considered a mechanism of cancer prevention. However, associations between PA and urinary levels of 8-hydroxydeoxyguanosine (8-OH-dG), which reflects DNA damage, are unclear. This cross-sectional study included 2370 men and 4052 women aged 45-74 years enrolled between 2010 and 2012. Habitual PA was assessed by single-axis accelerometer and urinary 8-OH-dG levels by automated HPLC. Multiple linear regression analysis was used to examine the relationship between log-transformed urinary 8-OH-dG and total PA (TPA) and PA of moderate/vigorous intensity (MVPA; ≥3 metabolic equivalents), with adjustment for age, body mass index, energy intake, alcohol consumption, smoking status, daily coffee drinking, menopause status (in women), and TPA (for MVPA). On multivariate adjustment, urinary 8-OH-dG levels were inversely correlated with TPA (β = -0.020, P < 0.01) in women, and this correlation was not changed by PA intensity. Conversely, urinary 8-OH-dG levels were inversely correlated with MVPA (β = -0.022, P < 0.05) in men, although the correlation with TPA was non-significant. This inverse correlation was clearer in current smokers than in never or former smokers, although the interaction between smoking status and MVPA on urinary 8-OH-dG levels was non-significant. In conclusion, greater TPA in women and greater MVPA in men were correlated with reduction in urinary 8-OH-dG, suggesting sex-specific effects of MVPA and TPA on protection from oxidative DNA damage. Increasing PA may mediate reduction in oxidative stress.
authors: Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan.
authors: Megumi
authors: M
authors: Hara
authors: Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan.
authors: Yuichiro
authors: Y
authors: Nishida
authors: Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan.
authors: Chisato
authors: C
authors: Shimanoe
authors: Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan.
authors: Yasuko
authors: Y
authors: Otsuka
authors: Department of Public Health, Showa University, Shinagawa, Tokyo, Japan.
authors: Hinako
authors: H
authors: Nanri
authors: Laboratory of Exercise Physiology, Faculty of Sports and Health Science, Fukuoka University, Fukuoka, Japan.
authors: Jun
authors: J
authors: Yasukata
authors: Department of Childhood Care Education, Seika Women's Junior College, Fukuoka, Japan.
authors: Nobuyuki
authors: N
authors: Miyoshi
authors: Department of Nutritional Science, National Institute of Health and Nutrition, Tokyo, Japan.
authors: Yosuke
authors: Y
authors: Yamada
authors: Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan.
authors: Mikako
authors: M
authors: Horita
authors: Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors: Kazuaki
authors: K
authors: Kawai
authors: Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors: Yun-Shan
authors: YS
authors: Li
authors: OHG Institute Co., Ltd., Kitakyushu, Japan.
authors: Jun
authors: J
authors: Kasai
authors: Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan.|OHG Institute Co., Ltd., Kitakyushu, Japan.
authors: Hiroshi
authors: H
authors: Kasai
authors: Laboratory of Exercise Physiology, Faculty of Sports and Health Science, Fukuoka University, Fukuoka, Japan.
authors: Yasuki
authors: Y
authors: Higaki
authors: Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan.
authors: Keitaro
authors: K
authors: Tanaka
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D061725:Accelerometry; D000328:Adult; D000368:Aged; D000428:Alcohol Drinking / Q000453:epidemiology; D015992:Body Mass Index; D063646:Carcinogenesis / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D002149:Energy Intake; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012907:Smoking / Q000453:epidemiology; D011795:Surveys and Questionnaires
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: Accelerometry; DNA damage; deoxyguanosine; exercise; oxidative stress