title: Bacterial succession and metabolite changes during flax (Linum usitatissimum L.) retting with Bacillus cereus HDYM-02.
abstract: High-throughput sequencing and GC-MS (gas chromatography-mass spectrometry) were jointly used to reveal the bacterial succession and metabolite changes during flax (Linum usitatissimum L.) retting. The inoculation of Bacillus cereus HDYM-02 decreased bacterial richness and diversity. This inoculum led to the replacement of Enterobacteriaceae by Bacillaceae. The level of aerobic Pseudomonadaceae (mainly Azotobacter) and anaerobic Clostridiaceae_1 gradually increased and decreased, respectively. Following the addition of B. cereus HDYM-02, the dominant groups were all degumming enzyme producers or have been proven to be involved in microbial retting throughout the entire retting period. These results could be verified by the metabolite changes, either degumming enzymes or their catalytic products galacturonic acid and reducing sugars. The GC-MS data showed a clear separation between flax retting with and without B. cereus HDYM-02, particularly within the first 72 h. These findings reveal the important bacterial groups that are involved in fiber retting and will facilitate improvements in the retting process.
authors: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.|Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, China.
authors: Dan
authors: D
authors: Zhao
authors: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.
authors: Pengfei
authors: P
authors: Liu
authors: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.
authors: Chao
authors: C
authors: Pan
authors: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.
authors: Renpeng
authors: R
authors: Du
authors: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.|Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, China.
authors: Wenxiang
authors: W
authors: Ping
authors: Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, China.|Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, China.
authors: Jingping
authors: J
authors: Ge
mesh_terms: D001420:Bacteria, Aerobic / Q000145:classification* / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000145:classification* / Q000378:metabolism*; D001688:Biological Products / Q000032:analysis; D058448:Biota; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D019597:Flax / Q000378:metabolism* / Q000382:microbiology*; D008401:Gas Chromatography-Mass Spectrometry; D059014:High-Throughput Nucleotide Sequencing
chemical_list: D001688:Biological Products; D004269:DNA, Bacterial