title: Construction of Epidermal Growth Factor Receptor Peptide Magnetic Nanovesicles with Lipid Bilayers for Enhanced Capture of Liver Cancer Circulating Tumor Cells.
abstract: Highly effective targeted tumor recognition via vectors is crucial for cancer detection. In contrast to antibodies and proteins, peptides are direct targeting ligands with a low molecular weight. In the present study, a peptide magnetic nanovector platform containing a lipid bilayer was designed using a peptide amphiphile (PA) as a skeleton material in a controlled manner without surface modification. Fluorescein isothiocyanate-labeled epidermal growth factor receptor (EGFR) peptide nanoparticles (NPs) could specifically bind to EGFR-positive liver tumor cells. EGFR peptide magnetic vesicles (EPMVs) could efficiently recognize and separate hepatoma carcinoma cells from cell solutions and treated blood samples (ratio of magnetic EPMVs versus anti-EpCAM NPs: 3.5 ± 0.29). Analysis of the circulating tumor cell (CTC) count in blood samples from 32 patients with liver cancer showed that EPMVs could be effectively applied for CTC capture. Thus, this nanoscale, targeted cargo-packaging technology may be useful for designing cancer diagnostic systems.
authors: Digestive Department, The First Affiliated Hospital of Fujian Medical University , 20 Chazhong Road, Fuzhou 350005, China.
authors: Jian
authors: J
authors: Ding
authors: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine , No.25/Ln2200 Xie Tu Road, Shanghai 200032, China.
authors: Kai
authors: K
authors: Wang
authors: Research Centre for Translational Medicine, East Hospital, Tongji University School of Medicine , 150 Jimo Road, Shanghai 200120, China.
authors: Wen-Jie
authors: WJ
authors: Tang
authors: Digestive Department, Union Hospital of Fujian Medical University , Fuzhou 350001, China.
authors: Dan
authors: D
authors: Li
authors: Research Centre for Translational Medicine, East Hospital, Tongji University School of Medicine , 150 Jimo Road, Shanghai 200120, China.
authors: You-Zhen
authors: YZ
authors: Wei
authors: Research Centre for Translational Medicine, East Hospital, Tongji University School of Medicine , 150 Jimo Road, Shanghai 200120, China.
authors: Ying
authors: Y
authors: Lu
authors: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine , No.25/Ln2200 Xie Tu Road, Shanghai 200032, China.
authors: Zong-Hai
authors: ZH
authors: Li
authors: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine , No.25/Ln2200 Xie Tu Road, Shanghai 200032, China.
authors: Xiao-Fei
authors: XF
authors: Liang
mesh_terms: D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism; D008113:Liver Neoplasms / Q000097:blood / Q000473:pathology*; D059346:Magnets / Q000737:chemistry; D053769:Nanocapsules / Q000737:chemistry; D009360:Neoplastic Cells, Circulating / Q000378:metabolism / Q000473:pathology*; D010455:Peptides / Q000378:metabolism*
chemical_list: D008051:Lipid Bilayers; D053769:Nanocapsules; D010455:Peptides; C512478:EGFR protein, human; D066246:ErbB Receptors