title: CGRP and Visceral Pain: The Role of Sex Hormones in In Vitro Experiment.
abstract: A large number of studies have showed that women reported feeling pain more acutely than men. In support of this hypothesis, many research groups proved that in different animals model of pain the sex hormones regulate the somatic and visceral sensitivity to different noxious stimuli. Therefore, in this study, we went to evaluate if estrogen hormones by regulating the CGRP levels are implicated during the visceral pain transmission. Toward this aim, we have investigated the effect of 17β-estradiol in regulating the synthesis and release of CGRP, as well as the expression levels of the opioid receptor of type K. In order to gain information about the potential effects of 17β-estradiol on K-opioid receptor expression and activity, we have cultured F11 cells. Our results revealed that, when F11 cells were short-term exposed (30 min) to 17β-estradiol, the expression of the opioid K receptor was not significantly modified. We carried out enzyme immunoassay analysis to evaluate the potential effects of short-term exposure to 17-estradiol (30 min) on the release of CGRP in F11 cells. The results obtained showed that 17β-estradiol at the dose of 100 nM is able to induce the release of CGRP from F11 cells; whereas, a higher dose of 17β-estradiol (200 nM) did not produce significant effects when compared to control. In conclusion, all these findings suggest that the 17β-estradiol-regulated release of CGRP could at least in part provide a rational explanation for the difference of gender in the visceral pain sensitivity. J. Cell. Biochem. 118: 510-517, 2017. © 2016 Wiley Periodicals, Inc.
authors: Department of Anaesthesiological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors: Vincenzo
authors: V
authors: Pota
authors: Department of Anaesthesiological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors: Vincenzo
authors: V
authors: Quagliariello
authors: Department of Anaesthesiological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors: Emilia
authors: E
authors: Armenia
authors: Department of Anaesthesiological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors: Caterina
authors: C
authors: Aurilio
authors: Department of Anaesthesiological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors: Maria Beatrice
authors: MB
authors: Passavanti
authors: Department of Anaesthesiological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors: Pasquale
authors: P
authors: Sansone
authors: Department of Anaesthesiology, ASL Salerno, Salerno, Italy.
authors: Mario
authors: M
authors: Iannotti
authors: Department of Engine, Second University of Naples, Naples, Italy.
authors: Michelina
authors: M
authors: Catauro
authors: Perugia University School of Medicine-District of Terni, Institute of Internal Medicine, Rheumatology and Medical Pain Therapy, Perugia, Italy.
authors: Stefano
authors: S
authors: Coaccioli
authors: Department of Neurosurgery, Second University of Naples, Naples, Italy.
authors: Manlio
authors: M
authors: Barbarisi
authors: Department of Anaesthesiological, Surgical and Emergency Sciences, Second University of Naples, Naples, Italy.
authors: Maria Caterina
authors: MC
authors: Pace
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D059265:Visceral Pain / Q000378:metabolism*
chemical_list: D004958:Estradiol; D015740:Calcitonin Gene-Related Peptide
keywords: CGRP; GENDER; VISCERAL PAIN