title: SfP53 and filamentous actin (F-actin) are the targets of viral pesticide AcMNPV-BmK IT (P10/PH) in host Spodoptera frugiperda 9 cells.
abstract: OBJECTIVE
  To analyze the anti-insect mechanism of viral pesticide AcMNPV-BmK IT(P10/PH) in the host Spodoptera frugiperda 9 (Sf9) cells.
  
  
  RESULTS
  Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV)- mediated expression of BmK IT, regulated by P10 protein promoter (P10) and polyhedrosis promoter (PH), promoted the replication of progeny virus in host Sf9 cells. AcMNPV-BmK IT(P10) could accelerate the budding process (or speed) of budded virus (BV) in Sf9 cells. The impact of AcMNPV-BmK IT(P10) on the nuclear polymerization of filamentous actin (F-actin) participated in regulating the accelerated budding process. Unexpectedly, both AcMNPV-BmK IT(P10) and AcMNPV-BmK IT(PH) delayed the nuclear polymerization of F-actin and promoted the clearance of F-actin in the nucleus. SfP53, an important apoptosis factor, was involved in the regulation of AcMNPV-BmK IT(P10/PH) in Sf9 cells. AcMNPV-BmK IT(P10/PH) could also delay and promote the nuclear recruitment of SfP53 after 27 h post infection (h p.i.).
  
  
  CONCLUSIONS
  SfP53 and F-actin are the targets of viral pesticide AcMNPV-BmK IT (P10/PH) in host Sf9 cells, which provides the experimental basis for the development of recombinant baculovirus biopesticides.
authors: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China.
authors: Leixi
authors: L
authors: Cao
authors: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China.
authors: Xing
authors: X
authors: Li
authors: University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
authors: Shuhua
authors: S
authors: Zheng
authors: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China.
authors: Aihua
authors: A
authors: Liang
authors: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China. yjfu@sxu.edu.cn.
authors: Yuejun
authors: http://orcid.org/0000-0002-9575-7019
authors: Y
authors: Fu
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D017924:Nucleopolyhedroviruses / Q000378:metabolism / Q000472:pathogenicity; D010575:Pesticides / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D061987:Sf9 Cells
chemical_list: D000199:Actins; D010575:Pesticides; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases
keywords: AcMNPV-BmK IT(P10/PH); F-Actin; Nuclear polymerization; Polyhedrosis promoter; Spodoptera frugiperda; Viral pesticide