title: Spring viraemia of carp virus enters grass carp ovary cells via clathrin-mediated endocytosis and macropinocytosis.
abstract: Spring viraemia of carp virus (SVCV) is the causative pathogen of the outbreaks of an acute haemorrhagic and contagious viraemia responsible for the significant mortality in several cyprinid species. However, the endocytic pathway(s) and their regulatory molecules have not been characterized for SVCV. Here, using a combination of specific pharmacological inhibitors, transmission electron microscopy, immunofluorescence microscopy and real-time quantitative PCR, we found that SVCV entered grass carp ovary cells via clathrin-mediated endocytosis and macropinocytosis in a low-pH-dependent manner. We also discovered that dynamin II, actin microfilaments and microtubules were essential for SVCV internalization. Moreover, we found that the P21-activated kinase 1 inhibitor IPA-3 and the protein kinase C inhibitor rottlerin could block SVCV cell entry and replication, while phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 could promote SVCV infection. Results presented in this study provide helpful insight into revealing the initial steps of SVCV infection, and they may facilitate the development of therapeutic interventions.
authors: Shanghai Fisheries Research Institute, Shanghai Fisheries Technical Extension Station, Shanghai 200433, PR China.
authors: Ling
authors: L
authors: Shao
authors: Institute of Embryo-Fetal Original Adult Disease Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200030, PR China.
authors: Jiuru
authors: J
authors: Zhao
authors: Shanghai Fisheries Research Institute, Shanghai Fisheries Technical Extension Station, Shanghai 200433, PR China.
authors: Haiqiang
authors: H
authors: Zhang
mesh_terms: D000818:Animals; D002347:Carps / Q000821:virology*; D002966:Clathrin / Q000378:metabolism*; D004705:Endocytosis; D005260:Female; D005393:Fish Diseases / Q000378:metabolism / Q000503:physiopathology / Q000821:virology*; D029941:Fish Proteins / Q000378:metabolism*; D010053:Ovary / Q000821:virology*; D018116:Vesiculovirus / Q000502:physiology*; D014766:Viremia / Q000378:metabolism / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D053586:Virus Internalization
chemical_list: D002966:Clathrin; D029941:Fish Proteins