title: Identification of antimicrobial activity among new sulfonamide metal complexes for combating rapidly growing mycobacteria.
abstract: Mycobacteriosis is a type of infection caused by rapidly growing mycobacteria (RGM), which can vary from localized illness, such as skin disease, to disseminated disease. Amikacin, cefoxitin, ciprofloxacin, clarithromycin, doxycycline, imipenem and sulfamethoxazole are antimicrobial drugs chosen to treat such illnesses; however, not all patients obtain the cure. The reason why the treatment does not work for those patients is related to the fact that some clinical strains present resistance to the existing antimicrobial drugs; thereby, the research of new therapeutic approaches is extremely relevant. The coordination of antimicrobial drugs to metals is a promising alternative in the development of effective compounds against resistant microorganisms. Sulfonamides complexed with Au, Cd, Ag, Cu, and Hg have shown excellent activity against a variety of microorganisms. Considering the importance of fighting against infections associated with RGM, the objective of this study is to evaluate the antimycobacterial activity of metal complexes of sulfonamides against RGM. Complexed sulfonamides activity were individually tested and in association with trimethoprim. The minimum inhibitory concentration (MIC) and time-kill curve of compounds against the standard strains of RGM [Mycobacterium abscessus (ATCC 19977), Mycobacterium fortuitum (ATCC 6841) and Mycobacterium massiliense (ATCC 48898)] was determined. The interaction of sulfonamides with trimethoprim was defined by inhibitory concentration index fractional for each association. The results showed that sulfonamides complexed whit metals have outstanding antimicrobial activity when compared to free sulfamethoxazole, bactericidal activity and synergistic effect when combined with trimethoprim.
authors: Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, 1000 Avenida Roraima, Santa Maria, RS, 97105900, Brazil.
authors: Vanessa Albertina
authors: VA
authors: Agertt
authors: Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, 1000 Avenida Roraima, Santa Maria, RS, 97105900, Brazil.
authors: Pauline Cordenonsi
authors: PC
authors: Bonez
authors: Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, 1000 Avenida Roraima, Santa Maria, RS, 97105900, Brazil.
authors: Grazielle Guidolin
authors: GG
authors: Rossi
authors: Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, 1000 Avenida Roraima, Santa Maria, RS, 97105900, Brazil.
authors: Vanessa da Costa
authors: Vda C
authors: Flores
authors: Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, 1000 Avenida Roraima, Santa Maria, RS, 97105900, Brazil.
authors: Fallon Dos Santos
authors: Fdos S
authors: Siqueira
authors: Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, 1000 Avenida Roraima, Santa Maria, RS, 97105900, Brazil.
authors: Caren Rigon
authors: CR
authors: Mizdal
authors: Centro de Ciências Naturais e Exatas, Departamento de Química, Universidade Federal de Santa Maria, 1000 Avenida Roraima, Santa Maria, RS, 9710597, Brazil.
authors: Lenice Lorenço
authors: LL
authors: Marques
authors: Centro de Ciências Naturais e Exatas, Departamento de Química, Universidade Federal de Santa Maria, 1000 Avenida Roraima, Santa Maria, RS, 9710597, Brazil.
authors: Gelson Noe Manzoni
authors: GN
authors: de Oliveira
authors: Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, 1000 Avenida Roraima, Santa Maria, RS, 97105900, Brazil. marlimatiko@yahoo.com.
authors: Marli Matiko Anraku
authors: MM
authors: de Campos
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D019216:Metals, Heavy / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000187:drug effects* / Q000254:growth & development*; D013329:Structure-Activity Relationship; D013449:Sulfonamides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D056831:Coordination Complexes; D019216:Metals, Heavy; D013449:Sulfonamides
keywords: Metal complexes; Rapidly growing mycobacteria; Sulfamethoxazole; Susceptibility; Trimethoprim