title: Evaluation of the Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Gram-Negative Bacteria in Comparison with the RAPIDEC CARBA NP.
abstract: Timely detection of carbapenemases by both phenotypic and genotypic methods is essential for developing strategies to control the spread of infections by carbapenem-resistant isolates and related morbidity and mortality. The aim of this study was to compare the performance of a commercial kit, the RAPIDEC®CARBA NP, and an in-house technique, the carbapenem inactivation method (CIM), against a panel of 136 carbapenemase- and noncarbapenemase-producing Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa isolates. RAPIDEC CARBA NP displayed 99% sensitivity and 100% specificity, whereas the sensitivity and specificity were 78% and 100% for the CIM test, respectively. A slight modification of the CIM test, a prolonged incubation time of 4 hours instead of two, increased the sensitivity of the test to 90% by diminishing false negativity particularly for A. baumannii. In conclusion, both tests possess a high performance and are practical for the detection of carbapenemases. Although RAPIDEC CARBA NP is a more rapid and reliable method, the CIM test may represent a useful tool for microbiology laboratories due to its simplicity and availability at any laboratory with low cost.
authors: 1 Clinical Microbiology Laboratory, Şişli Hamidiye Etfal Training and Research Hospital , Istanbul, Turkey .
authors: Elif
authors: E
authors: Aktaş
authors: 2 Kocaeli Public Health Laboratory , Kocaeli, Turkey .
authors: Gülşah
authors: G
authors: Malkoçoğlu
authors: 3 Department of Medical Microbiology, Faculty of Medicine, İnönü University , Malatya, Turkey .
authors: Barış
authors: B
authors: Otlu
authors: 4 Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdoğan University , Rize, Turkey .
authors: Ayşegül
authors: A
authors: Çopur Çiçek
authors: 5 Department of Medical Microbiology, Faculty of Medicine, Bülent Ecevit University Zonguldak , Turkey .
authors: Canan
authors: C
authors: Külah
authors: 5 Department of Medical Microbiology, Faculty of Medicine, Bülent Ecevit University Zonguldak , Turkey .
authors: Füsun
authors: F
authors: Cömert
authors: 6 Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdoğan University , Rize, Turkey .
authors: Cemal
authors: C
authors: Sandallı
authors: 3 Department of Medical Microbiology, Faculty of Medicine, İnönü University , Malatya, Turkey .
authors: Nafia Canan
authors: NC
authors: Gürsoy
authors: 1 Clinical Microbiology Laboratory, Şişli Hamidiye Etfal Training and Research Hospital , Istanbul, Turkey .
authors: Duygu
authors: D
authors: Erdemir
authors: 1 Clinical Microbiology Laboratory, Şişli Hamidiye Etfal Training and Research Hospital , Istanbul, Turkey .
authors: Mehmet Emin
authors: ME
authors: Bulut
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression; D008658:Inactivation, Metabolic; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012680:Sensitivity and Specificity; D013845:Thienamycins / Q000378:metabolism* / Q000494:pharmacology; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; D013845:Thienamycins; D001618:beta-Lactamases; C063912:carbapenemase; D000077731:Meropenem
keywords: CARBA NP; CIM; carbapenemase