title: Association of the CETP Taq1B and LIPG Thr111Ile Polymorphisms with Glycated Hemoglobin and Blood Lipids in Newly Diagnosed Hyperlipidemic Patients.
abstract: OBJECTIVE
  To examine the association of 2 common polymorphisms in high-density lipoprotein (HDL)-related genes, namely, cholesterol ester transfer protein CETP Taq1B (rs708272) and endothelial lipase LIPG Thr111Ile (rs2000813), with glycated hemoglobin (A1C), blood lipid levels and the risk for type 2 diabetes in a group of hyperlipidemic patients from northern Greece.
  
  
  METHODS
  We categorized 175 patients with hyperlipidemia into 2 subgroups according to the presence or absence of type 2 diabetes, defined as a recent diagnosis, A1C >6.5% and/or fasting glucose >126 mg/dL. Genotypes for the 2 polymorphisms studied were determined by polymerase chain reaction-restriction fragment length polymorphism. Both polymorphisms were analyzed by multivariate and univariate analyses of baseline A1C levels and plasma lipids. The genotype and allele frequencies of the 2 subgroups were compared.
  
  
  RESULTS
  The CETP Taq1B polymorphism was associated with HDL-cholesterol (HDL-C) and A1C levels, but this association was affected by type 2 diabetes; the association with A1C levels was significant only in type 2 diabetes (p=0.005), whereas the association with HDL-C occurred only in the subgroup without type 2 diabetes (p<0.001). LIPG Thr111Ile did not affect plasma HDL-C or A1C levels independently but appeared to modulate their association with CETP Taq1B, and LIPG 111IleIle homozygotes tended to be present at a higher frequency in the hyperlipidemic patients with type 2 diabetes compared to the hyperlipidemic patients without type 2 diabetes (p=0.056).
  
  
  CONCLUSIONS
  In hyperlipidemic patients, apart from its known association with HDL-C, CETP Taq1B is also associated with A1C levels, and both associations are modified by type 2 diabetes and LIPG Thr111Ile.
authors: First Propedeutic Department of Internal Medicine, AHEPA Hospital, Department of Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors: Dimitrios
authors: D
authors: Agapakis
authors: First Propedeutic Department of Internal Medicine, AHEPA Hospital, Department of Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors: Christos
authors: C
authors: Savopoulos
authors: Pharmacology Unit, Department of Medicine, University of Patras School of Health Sciences, Patras, Greece.
authors: Kyriakos E
authors: KE
authors: Kypreos
authors: First Laboratory of Pharmacology, Department of Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors: Emma
authors: E
authors: Gbandi
authors: Laboratory of Biological Chemistry, Department of Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors: Stavros
authors: S
authors: Iliadis
authors: First Propedeutic Department of Internal Medicine, AHEPA Hospital, Department of Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors: Apostolos I
authors: AI
authors: Hatzitolios
authors: First Laboratory of Pharmacology, Department of Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece. Electronic address: goulas@med.auth.gr.
authors: Antonis
authors: A
authors: Goulas
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D053480:Cholesterol Ester Transfer Proteins / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000235:genetics*; D008049:Lipase / Q000235:genetics*; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D011379:Prognosis; D012189:Retrospective Studies; D019914:Taq Polymerase / Q000235:genetics*
chemical_list: D015415:Biomarkers; D053480:Cholesterol Ester Transfer Proteins; D006442:Glycated Hemoglobin; D008049:Lipase; D008055:Lipids; D019914:Taq Polymerase; C506642:CETP protein, human; C517652:hemoglobin A1c protein, human; C410382:LIPG protein, human
keywords: cholesteryl ester transfer protein; diabète de type 2; endothelial lipase; gene polymorphisms; glycated hemoglobin; lipase endothéliale; lipides plasmatiques; plasma lipids; polymorphismes des gènes, hémoglobine glyquée; protéine de transfert des esters de cholestérol; type 2 diabetes