title: Anti-adipogenic and anti-diabetic effects of cis-3',4'-diisovalerylkhellactone isolated from Peucedanum japonicum Thunb leaves in vitro.
abstract: Peucedanum japonicum Thunb is a medicinal plant belonging to the family Umbelliferae. This study evaluated the anti-diabetic and anti-obesity effects of cis-3',4'-diisovalerylkhellactone (cDIVK) isolated from Peucedanum japonicum Thunb leaves. cDIVK (30 and 50μM) effectively inhibited adipocyte differentiation and fat accumulation, whereas it stimulated glucose uptake compared with the control in 3T3-L1 cells. cDIVK significantly increased AMPK activation and suppressed protein and mRNA expression of major adipogenic transcriptional factors such as C/EBPα, PPARγ and SREBP-1c in 3T3-L1 cells. In addition, cDIVK had potential α-glucosidase inhibitory activity. These results indicated that cDIVK may act as a natural dual therapeutic agent for diabetes and obesity.
authors: Department of Food and Nutrition, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Republic of Korea.
authors: Ra-Yeong
authors: RY
authors: Choi
authors: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 03760, Republic of Korea; Suncheon Research Center for Natural Medicines, Suncheon 57922, Republic of Korea.
authors: Sang-Jip
authors: SJ
authors: Nam
authors: Department of Food and Nutrition, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Republic of Korea.
authors: Ju Ri
authors: JR
authors: Ham
authors: Mokpo Marin Food-Industry Research Center, Mokpo 58621, Republic of Korea.
authors: Hae-In
authors: HI
authors: Lee
authors: College of Pharmacy, Sunchon National University, Suncheon 57922, Republic of Korea; Suncheon Research Center for Natural Medicines, Suncheon 57922, Republic of Korea.
authors: Sung-Tae
authors: ST
authors: Yee
authors: College of Pharmacy, Sunchon National University, Suncheon 57922, Republic of Korea.
authors: Kyung-Yun
authors: KY
authors: Kang
authors: Department of Biotechnology, Dong-A University, Busan 49315, Republic of Korea.
authors: Kwon-Il
authors: KI
authors: Seo
authors: Functional Food & Nutrition Division, National Institute of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea.
authors: Ju-Hye
authors: JH
authors: Lee
authors: Department of Bakery & Barista, Suseong College, Daegu 42078, Republic of Korea.
authors: Myung-Joo
authors: MJ
authors: Kim
authors: Department of Food and Nutrition, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Republic of Korea; Suncheon Research Center for Natural Medicines, Suncheon 57922, Republic of Korea. Electronic address: leemk@sunchon.ac.kr.
authors: Mi-Kyung
authors: MK
authors: Lee
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D019661:Apiaceae / Q000737:chemistry*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002454:Cell Differentiation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D051379:Mice; D018515:Plant Leaves / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet
chemical_list: C510221:3', 4'-diisovalerylkhellactone; D019440:Anti-Obesity Agents; D003374:Coumarins; D007004:Hypoglycemic Agents
keywords: Adipocytes; Adipogenesis; Anti-diabetes; Anti-obesity; Peucedanum japonicum Thunb leaves