title: Functional evaluation of rat hearts transplanted after preservation in a high-pressure gaseous mixture of carbon monoxide and oxygen.
abstract: We recently succeeded in resuscitating an extracted rat heart following 24-48 hours of preservation in a high-pressure gaseous mixture of carbon monoxide (CO) and oxygen (O2). This study aimed to examine the function of rat hearts transplanted after being preserved in the high-pressure CO and O2 gas mixture. The hearts of donor rats were preserved in a chamber filled with CO and O2 under high pressure for 24 h (CO24h) or 48 h at 4 °C. For the positive control (PC) group, hearts immediately extracted from donor rats were used for transplantation. The preserved hearts were transplanted into recipient rats by heterotopic cervical heart transplantation. CO toxicity does not affect the grafts or the recipients. Light microscopy and [(18)F]-fluorodeoxyglucose positron emission tomography revealed that there were no significant differences in the size of the myocardial infarction or apoptosis of myocardial cells in post-transplant hearts between the PC and CO24h groups. Furthermore, at 100 days after the transplantation, the heart rate, weight and histological staining of the post-transplanted hearts did not differ significantly between the PC and CO24h groups. These results indicate that the function of rat hearts is well preserved after 24 hours of high-pressure preservation in a CO and O2 gas mixture. Therefore, high-pressure preservation in a gas mixture can be a useful method for organ preservation.
authors: Department of Anatomy, Tokyo Medical University, Tokyo, Japan.|Department of Anatomy, Aichi Medical University, 1-1 Yazakokarimata, Nagakute-city, Aichi Pref., 480-1195, Japan.
authors: Naoyuki
authors: N
authors: Hatayama
authors: Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan.|Department of Nuclear Medicine, Kawasaki Medical School, Kurashiki, Japan.
authors: Masayuki
authors: M
authors: Inubushi
authors: Department of Anatomy, Tokyo Medical University, Tokyo, Japan.|Department of Anatomy, Aichi Medical University, 1-1 Yazakokarimata, Nagakute-city, Aichi Pref., 480-1195, Japan.
authors: Munekazu
authors: M
authors: Naito
authors: Department of Anatomy, Tokyo Medical University, Tokyo, Japan.
authors: Shuichi
authors: S
authors: Hirai
authors: Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan.
authors: Yong-Nan
authors: YN
authors: Jin
authors: Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan.
authors: Atsushi B
authors: AB
authors: Tsuji
authors: High Altitude Pathology Institute, La Paz, Bolivia.
authors: Kunihiro
authors: K
authors: Seki
authors: Department of Anatomy, Tokyo Medical University, Tokyo, Japan.
authors: Masahiro
authors: M
authors: Itoh
authors: Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan.
authors: Tsuneo
authors: T
authors: Saga
authors: National Research Institute for Child Health and Development, Tokyo, Japan.
authors: Xiao-Kang
authors: XK
authors: Li
mesh_terms: D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology*; D016027:Heart Transplantation; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000209:etiology / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009926:Organ Preservation / Q000379:methods*; D010100:Oxygen / Q000494:pharmacology*; D049268:Positron-Emission Tomography; D051381:Rats
chemical_list: D002248:Carbon Monoxide; D010100:Oxygen