title: Effect of different metals on oxidative state and mitochondrial membrane potential in trout erythrocytes.
abstract: Homeostasis of metal ions is critical for life and excessive exposure can promote cellular damage that could be due to oxidative damage. In this context we evaluated the effects of three different elements (copper, zinc and aluminum) on oxidative stress and mitochondrial functionality in nucleated trout erythrocytes (Oncorhynchus mykiss). Flowcytometric measurements using MitoProbe and DCFDA-H2as fluorescent probes, indicated that redox active copper was able to influence all the biological parameters considered while redox inert, zinc and aluminum, show no significant effects. Toxicity of Al and Zn represent a debated argument and their ability to interact with other endogenous metal ions/metal binding proteins could play a role modulating their cellular toxicity.
authors: Department of Clinical Dental Sciences, Polytechnic University of Marche, Ancona, Italy.
authors: Sonia
authors: S
authors: Silvestri
authors: Department of Clinical Dental Sciences, Polytechnic University of Marche, Ancona, Italy.
authors: Patrick
authors: P
authors: Orlando
authors: Department of Clinical Dental Sciences, Polytechnic University of Marche, Ancona, Italy.
authors: Francesca
authors: F
authors: Brugè
authors: School of Pharmacy and Health Products, University of Camerino, Italy.
authors: Giancarlo
authors: G
authors: Falcioni
authors: Department of Clinical Dental Sciences, Polytechnic University of Marche, Ancona, Italy. Electronic address: l.tiano@univpm.it.
authors: Luca
authors: L
authors: Tiano
keywords: Flow cytometry; Haemoglobin; Metals; Mitochondrial membrane potential; Reactive oxygen species; Trout erythrocytes