title: [Role of epithelial sodium channel in rat osteoclast differentiation and bone resorption].
abstract: OBJECTIVE
  To explore the role of epithelial sodium channel (ENaC) in regulating the functional activity of osteoclasts.
  
  
  METHODS
  Multinucleated osteoclasts were obtained by inducing the differentiation of rat bone marrow cells with macrophage colony-stimulating factor (M-CSF) and RANKL. The osteoclasts were exposed to different concentrations of the ENaC inhibitor amiloride, and the expression of ENaC on osteoclasts was examined using immunofluorescence technique. The osteoclasts were identified with tartrate-resistant acid phosphatase (TRAP) staining, and the positive cells were incubated with fresh bovine femoral bone slices and the number of bone absorption pits was counted by computer-aided image processing. RT-PCR was performed to analyze the expression of cathepsin K in the osteoclasts.
  
  
  RESULTS
  s Exposure to different concentrations of amiloride significantly inhibited the expression of ENaC and reduced the number of TRAP-positive osteoclasts. Exposure of the osteoclasts to amiloride also reduced the number of bone resorption pits on bone slices and the expression of osteoclast-specific gene cathepsin K.
  
  
  CONCLUSIONS
  s ENaC may participate in the regulation of osteoclast differentiation and bone resorption, suggesting its role in functional regulation of the osteoclasts and a possibly new signaling pathway related with ENaC regulation for modulating bone metabolism.
authors: Guangdong Food and Drug Vocational College, Guangzhou 510520, China. E-mail: 759949207 @qq.com.
authors: Song-Yan
authors: SY
authors: Hu
authors: Xiao-Dong
authors: XD
authors: Jin
authors: Hao
authors: H
authors: Zhang
authors: Jun
authors: J
authors: Chen
authors: Guo-Zhu
authors: GZ
authors: Yang
authors: Xiao-Dong
authors: XD
authors: Wang
authors: Lu
authors: L
authors: Tang
authors: Xing-Yan
authors: XY
authors: Lu
authors: Li
authors: L
authors: Lu
authors: Qing-Nan
authors: QN
authors: Li
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D001862:Bone Resorption; D056657:Cathepsin K / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation; D053503:Epithelial Sodium Channels / Q000378:metabolism*; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology*; D053245:RANK Ligand / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction
chemical_list: D053503:Epithelial Sodium Channels; D053245:RANK Ligand; D016173:Macrophage Colony-Stimulating Factor; D056657:Cathepsin K