title: A slow excitatory postsynaptic current mediated by a novel metabotropic glutamate receptor in CA1 pyramidal neurons.
abstract: Slow excitatory postsynaptic currents (EPSCs) mediated by metabotropic glutamate receptors (mGlu receptors) have been reported in several neuronal subtypes, but their presence in hippocampal pyramidal neurons remains elusive. Here we find that in CA1 pyramidal neurons a slow EPSC is induced by repetitive stimulation while ionotropic glutamate receptors and glutamate-uptake are blocked whereas it is absent in the VGLUT1 knockout mouse in which presynaptic glutamate is lost, suggesting the slow EPSC is mediated by glutamate activating mGlu receptors. However, it is not inhibited by known mGlu receptor antagonists. These findings suggest that this slow EPSC is mediated by a novel mGlu receptor, and that it may be involved in neurological diseases associated with abnormal high-concentration of extracellular glutamate. This article is part of the Special Issue entitled 'Metabotropic Glutamate Receptors, 5 years on'.
authors: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, United States.
authors: Nengyin
authors: N
authors: Sheng
authors: Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, United States; Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, United States.
authors: Jing
authors: J
authors: Yang
authors: Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, United States; Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, United States.
authors: Katlin
authors: K
authors: Silm
authors: Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, United States; Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, United States.
authors: Robert H
authors: RH
authors: Edwards
authors: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, United States; Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, United States. Electronic address: roger.nicoll@ucsf.edu.
authors: Roger A
authors: RA
authors: Nicoll
mesh_terms: D000818:Animals; D001565:Benzoates / Q000494:pharmacology; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000502:physiology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000037:antagonists & inhibitors / Q000502:physiology*; D050598:Vesicular Glutamate Transport Protein 1 / Q000037:antagonists & inhibitors / Q000172:deficiency*
chemical_list: D001565:Benzoates; D018691:Excitatory Amino Acid Antagonists; D018094:Receptors, Metabotropic Glutamate; C493465:Slc17a7 protein, mouse; D050598:Vesicular Glutamate Transport Protein 1; C079694:alpha-methyl-4-carboxyphenylglycine; D008734:Methoxyhydroxyphenylglycol; D005998:Glycine; C010117:3,4-dihydroxyphenylglycol
keywords: CA1 neuron; Glutamate uptake; Slow EPSC; TBOA; mGlu receptor