title: MiR-98 promotes chondrocyte apoptosis by decreasing Bcl-2 expression in a rat model of osteoarthritis.
abstract: Altered expression of miRNA-98 (miR-98) has been reported in osteoarthritis (OA) patients, while its role and underlying mechanisms remain elusive. In the present study, a rat model of OA was established using modified Hulth method, and the expression level of miR-98 and its effect on cartilage degradation and cell apoptosis in OA rats were examined. The results showed that up-regulated miR-98 was observed in OA rats, and knockdown of miR-98 in OA rats resulted in an inhibitory effect on cartilage degradation and chondrocyte apoptosis. Then the potential apoptosis associated genes regulated by miR-98 were screened and examined in cartilage tissues. The target gene of miR-98 was validated by luciferase reporter assay. The data showed that the increased miR-98 was accompanied with a reduced expression of Bcl-2 at both mRNA and protein levels. Furthermore, the silencing of miR-98 in OA rats prevented the down-regulation of Bcl-2 in cartilage tissues. Finally, the luciferase reporter assay validated that Bcl-2 was the target gene of miR-98. In this study, we found that miR-98 might promote chondrocyte apoptosis and cartilage degradation by down-regulating Bcl-2 expression in the pathogenesis of OA, suggesting that miR-98 can be a potential target for the treatment of OA.
authors: Department of Rheumatology and Immunology, First People's Hospital of Yunnan Province, Kunming 650032, China.
authors: Jing
authors: J
authors: Wang
authors: Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China.
authors: Lingqiang
authors: L
authors: Chen
authors: Department of Rheumatology and Immunology, First People's Hospital of Yunnan Province, Kunming 650032, China.
authors: Song
authors: S
authors: Jin
authors: Department of Rheumatology and Immunology, First People's Hospital of Yunnan Province, Kunming 650032, China.
authors: Jun
authors: J
authors: Lin
authors: Department of Rheumatology and Immunology, First People's Hospital of Yunnan Province, Kunming 650032, China.
authors: Hongmei
authors: H
authors: Zheng
authors: Department of Rheumatology and Immunology, First People's Hospital of Yunnan Province, Kunming 650032, China.
authors: Hong
authors: H
authors: Zhang
authors: Department of Rheumatology and Immunology, First People's Hospital of Yunnan Province, Kunming 650032, China.
authors: Hongtao
authors: H
authors: Fan
authors: Department of Rheumatology and Immunology, First People's Hospital of Yunnan Province, Kunming 650032, China.
authors: Fang
authors: F
authors: He
authors: Department of Rheumatology and Immunology, First People's Hospital of Yunnan Province, Kunming 650032, China.
authors: Sha
authors: S
authors: Ma
authors: Department of Rheumatology and Immunology, First People's Hospital of Yunnan Province, Kunming 650032, China liqinkhyy@163.com.
authors: Qin
authors: Q
authors: Li
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D015153:Blotting, Western; D002358:Cartilage, Articular / Q000378:metabolism; D019902:Chondrocytes / Q000378:metabolism*; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D010003:Osteoarthritis / Q000235:genetics* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D020413:3' Untranslated Regions; C571630:MIRN98 microRNA-98, rat; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2
keywords: Bcl-2; apoptosis; microRNA-98; osteoarthritis