title: Pure titanium particle loaded nanocomposites: study on the polymer/filler interface and hMSC biocompatibility.
abstract: The integration of inorganic nanoparticles into polymer matrices allows for the modification of physical properties as well as the implementation of new features for unexplored application fields. Here, we propose the study of a new metal/polymer nanocomposite fabricated by dispersing pure Ti nanoparticles into a poly(methylmetacrilate) matrix via solvent casting process, to investigate its potential use as new biomaterial for biomedical applications. We demonstrated that Ti nanoparticles embedded in the poly(methylmetacrilate) matrix can act as reinforcing agent, not negatively influencing the biological response of human mesenchymal stem cell in terms of cytotoxicity and cell viability. As a function of relative amount and surface treatment, Ti nanoparticles may enhance mechanical strength of the composite-ranging from 31.1 ± 2.5 to 43.7 ± 0.7 MPa-also contributing to biological response in terms of adhesion and proliferation mechanisms. In particular, for 1 wt% Ti, treated Ti nanoparticles improve cell materials recognition, as confirmed by higher cell spreading-quantified in terms of cell area via image analysis-locally promoting stronger interactions at cell matrix interface. At this stage, these preliminary results suggest a promising use of pure Ti nanoparticles as filler in polymer composites for biomedical applications.
authors: Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, Via Campi Flegrei 34, 80078, Pozzuoli, Italy.
authors: Roberto
authors: R
authors: Avolio
authors: Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, Mostra d'Oltremare, Pad.20, V.le Kennedy 54, 80125, Naples, Italy.
authors: Marietta
authors: M
authors: D'Albore
authors: Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, Mostra d'Oltremare, Pad.20, V.le Kennedy 54, 80125, Naples, Italy. vguarino@unina.it.
authors: Vincenzo
authors: V
authors: Guarino
authors: Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, Via Campi Flegrei 34, 80078, Pozzuoli, Italy.
authors: Gennaro
authors: G
authors: Gentile
authors: Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, Via Campi Flegrei 34, 80078, Pozzuoli, Italy.
authors: Maria Cristina
authors: MC
authors: Cocca
authors: Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, Mostra d'Oltremare, Pad.20, V.le Kennedy 54, 80125, Naples, Italy.
authors: Stefania
authors: S
authors: Zeppetelli
authors: Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, Via Campi Flegrei 34, 80078, Pozzuoli, Italy.
authors: Maria Emanuela
authors: ME
authors: Errico
authors: Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, Via Campi Flegrei 34, 80078, Pozzuoli, Italy.
authors: Maurizio
authors: M
authors: Avella
authors: Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, Mostra d'Oltremare, Pad.20, V.le Kennedy 54, 80125, Naples, Italy.|Department of Chemical Sciences & Materials Technology, National Research Council of Italy, P.le A. Moro, 7, 00185, Rome, Italy.
authors: Luigi
authors: L
authors: Ambrosio
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011312:Pressure; D012997:Solvents / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties; D013696:Temperature; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D012997:Solvents; D019904:Polymethyl Methacrylate; D014025:Titanium; D009584:Nitrogen; D010100:Oxygen