title: In vitro activity of plant extracts against biofilm-producing food-related bacteria.
abstract: The identification of effective antimicrobial agents also active on biofilms is a topic of crucial importance in food and industrial environment. For that purpose methanol extracts of Turkish plants, Ficus carica L., Juglans regia L., Olea europaea L., Punica granatum L. and Rhus coriaria L., were investigated. Among the extracts, P. granatum L. and R. coriaria L. showed the best antibacterial activity with minimum inhibitory concentrations (MIC) of 78-625μg/ml for Listeria monocytogenes and Staphylococcus aureus and 312-1250μg/ml for Escherichia coli and Pseudomonas aeruginosa. SubMICs produced a significant biofilm inhibition equal to 80-60% for L. monocytogenes and 90-80% for S. aureus. The extracts showed also the highest polyphenol content and the strongest antioxidant activity. Bioassay-guided and HPLC procedures demonstrated the presence of apigenin 4'-O-β-glucoside in P. granatum L. and myricetrin and quercitrin in R. coriaria L. Antigenotoxicity of plant extracts was also observed The present findings promote the value-adding of P. granatum L. and R. coriaria L. leaves as natural antimicrobial/antioxidant agents for control of food-related bacterial biofilms.
authors: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy. Electronic address: anostro@unime.it.
authors: Antonia
authors: A
authors: Nostro
authors: Department of Life Sciences and Biotechnology (SVeB), University of Ferrara, Ferrara, Italy.
authors: Alessandra
authors: A
authors: Guerrini
authors: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
authors: Andreana
authors: A
authors: Marino
authors: Department of Life Sciences and Biotechnology (SVeB), University of Ferrara, Ferrara, Italy.
authors: Massimo
authors: M
authors: Tacchini
authors: Department of Pharmacy, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
authors: Mara
authors: M
authors: Di Giulio
authors: Department of Life Sciences and Biotechnology (SVeB), University of Ferrara, Ferrara, Italy.
authors: Alessandro
authors: A
authors: Grandini
authors: Department of Biology, Science Faculty, Selcuk University, Campus, Konya, Turkey.
authors: Methap
authors: M
authors: Akin
authors: Department of Pharmacy, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
authors: Luigina
authors: L
authors: Cellini
authors: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
authors: Giuseppe
authors: G
authors: Bisignano
authors: Department of Biology, Science Faculty, Selcuk University, Campus, Konya, Turkey.
authors: Hatice T
authors: HT
authors: Saraçoğlu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D005516:Food Microbiology; D019684:Magnoliopsida / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000975:Antioxidants; D010936:Plant Extracts; D000432:Methanol
keywords: Antibacterial activity; Antioxidant activity; Bioautographic analysis; Biofilm; HPLC; Plant extracts