title: Analysis of Toll-like Receptor 9 Gene Polymorphisms in Sepsis.
abstract: OBJECTIVE
  To analyze the effect of TLR-9 (-1486 T>C) and TLR-9 (C>T) gene polymorphisms both at TLR-9 levels and together with their sepsis parameters. In this regard, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used in order to detect TLR-9 gene polymorphisms, whereas the ELISA technique was used to analyze TLR-9 serum levels in 80 sepsis patients and 100 healthy individuals.
  
  
  METHODS
  The study group consisted of 80 patients with a diagnosis of sepsis and 100 healthy individuals. TLR-9 C>T polymorphism was identified by PCR-RFLP.
  
  
  RESULTS
  There was no substantial difference observed between sepsis and control groups in terms of TLR-9 (-1486 T>C) and TLR-9 (C>T) genotype and allele distribution. When serum TLR-9 levels were compared to TLR-9 (-1486 T>C) and TLR-9 (C>T) genotype and allele distribution, there was a statistically substantial decrease in TLR-9 serum levels of both TLR-9 (-1486 T>C) TT and TLR-9 (C>T) TT individuals in the sepsis group (p=0.011 and p=0.036, respectively).
  
  
  CONCLUSIONS
  There is no relation between sepsis and both TLR-9 (C>T) and TLR-9(-1486 T>C) polymorphisms; however, there is a relation between sepsis and decreased serum TLR-9 levels of both TLR-9 (-1486 T>C) TT and TLR-9 (C>T) TT polymorphisms due to sepsis-associated immunosuppression.
authors: Programme of Anesthesiology, Marmara University Vocational School of Health Services, Istanbul, Turkey.
authors: Nazan
authors: N
authors: Atalan
authors: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors: Hande
authors: H
authors: Karagedik
authors: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors: Leyla
authors: L
authors: Acar
authors: Department of Cardiovascular Surgery, Marmara University Pendik Education and Research Hospital, Istanbul, Turkey.
authors: Selim
authors: S
authors: Isbir
authors: Department of Molecular Medicine, Institute of Health Sciences, Faculty of Medicine, Yeditepe University, Istanbul Turkey.
authors: Seda Gulec
authors: SG
authors: Yilmaz
authors: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors: Arzu
authors: A
authors: Ergen
authors: Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul Turkey turgay.isbir@yeditepe.edu.tr tisbir@superonline.com.
authors: Turgay
authors: T
authors: Isbir
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D018805:Sepsis / Q000235:genetics* / Q000473:pathology; D051217:Toll-Like Receptor 9 / Q000235:genetics*
chemical_list: D051217:Toll-Like Receptor 9
keywords: Toll-like receptors; inflammation; polymorphism; sepsis