title: Isolation and Characterization of Low- vs. High-Density Neutrophils in Cancer.
abstract: Neutrophils are the most abundant of all white blood cells in the human circulation and serve as the first line of defense against microbial infections. Traditionally, neutrophils were viewed as a homogeneous population of myeloid cells. However, in recent years accumulating evidence has suggested that neutrophils are heterogeneous and that distinct neutrophil subsets may play very different roles. Here, we describe the methodology for isolation of high- and low-density neutrophils from the murine and human circulation using a density gradient and antibody based enrichment. We further describe the methodology for functional characterization of these different neutrophil subsets in the context of cancer.
authors: Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, 91120, Jerusalem, Israel.
authors: Jitka Y
authors: JY
authors: Sagiv
authors: Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, 91120, Jerusalem, Israel.
authors: Sandra
authors: S
authors: Voels
authors: Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, 91120, Jerusalem, Israel. zvikag@ekmd.huji.ac.il.
authors: Zvi
authors: Z
authors: Granot
mesh_terms: D000818:Animals; D015415:Biomarkers; D002469:Cell Separation; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D004195:Disease Models, Animal; D006801:Humans; D016130:Immunophenotyping; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology*; D020556:Neutrophil Infiltration / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D010587:Phagocytosis; D010641:Phenotype; D016897:Respiratory Burst / Q000276:immunology
chemical_list: D015415:Biomarkers
keywords: Cancer; Cytotoxicity; Density; Enrichment; Flow cytometry; Isolation; Neutrophils