title: Effect of swine leukocyte antigen-DQA gene variation on diarrhea in Large White, Landrace, and Duroc piglets.
abstract: Piglet diarrhea is one of the most common factors that affects the benefits of the swine industry. Although recent studies have shown that exon 2 of SLA-DQA is associated with piglet resistance to diarrhea, contributions of genetic variation in the additional exon coding regions of this gene remain unclear. Here, we investigated variation in exons 1, 3 and 4 of the SLA-DQA gene and evaluated their effects on diarrheal infection in 425 suckling piglets. No variation was identified in exon 1. In exon 3, there were eight alleles detected, generated by 14 single nucleotide polymorphisms (SNPs) and three nucleotide deletions, eight SNPs being newly identified. Four allele sequences and three SNPs were identified in exon 4, only one SNP being newly identified. Statistical analysis showed that the genotypes of exon 3 are significantly associated with piglet diarrhea; indeed, genotypes DQA*wb01/wb02 and wb04/wb05 are clearly associated with resistance to piglet diarrhea, as they have the lowest probabilities of infection (P < 0.05). However, no significant association was found between the genotypes of exon 4 and diarrhea (P > 0.05). These results provide important new information concerning the level of genetic diversity at the SLA-DQA locus and suggest that further genetic association studies of piglet diarrhea resistance should include analyses of both exons 2 and 3 of this locus.
authors: College of Animal Science and Technology, Gansu Agricultural University, No. 1 Yingmen Village, Lanzhou, 730070, China.
authors: Q L
authors: QL
authors: Yang
authors: College of Animal Science and Technology, Gansu Agricultural University, No. 1 Yingmen Village, Lanzhou, 730070, China.
authors: X Y
authors: XY
authors: Huang
authors: College of Animal Science and Technology, Gansu Agricultural University, No. 1 Yingmen Village, Lanzhou, 730070, China.
authors: S G
authors: SG
authors: Zhao
authors: College of Life Science and Engineering, Northwest University for Nationalities, Northwest Village No.1, Chengguan District, Lanzhou, 730030, China.
authors: L X
authors: LX
authors: Liu
authors: College of Animal Science and Technology, Gansu Agricultural University, No. 1 Yingmen Village, Lanzhou, 730070, China.
authors: S W
authors: SW
authors: Zhang
authors: College of Animal Science and Technology, Gansu Agricultural University, No. 1 Yingmen Village, Lanzhou, 730070, China.
authors: W Z
authors: WZ
authors: Huang
authors: College of Animal Science and Technology, Gansu Agricultural University, No. 1 Yingmen Village, Lanzhou, 730070, China. gunsbao056@126.com.|Gansu Research Center for Swine Production Engineering and Technology, No. 1 Yingmen Village, Lanzhou, 730070, China. gunsbao056@126.com.
authors: S B
authors: SB
authors: Gun
mesh_terms: D000483:Alleles; D000818:Animals; D003967:Diarrhea / Q000235:genetics*; D060467:Disease Resistance / Q000235:genetics; D005091:Exons; D005787:Gene Frequency; D005838:Genotype; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics*; D013552:Swine / Q000235:genetics; D013553:Swine Diseases / Q000235:genetics*
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen
keywords: 
  SLA-DQA
  ; 
  SNP
  ; association; piglet diarrhea; resistance; sequence variation