title: Establishment and molecular characterisation of seven novel soft-tissue sarcoma cell lines.
abstract: BACKGROUND
  Soft-tissue sarcomas (STS) are a diverse group of malignancies that remain a diagnostic and therapeutic challenge. Relatively few reliable cell lines currently exist. Rapidly developing technology for genomic profiling with emerging insights into candidate functional (driver) aberrations raises the need for more models for in vitro functional validation of molecular targets.
  
  
  METHODS
  Primary cell culture was performed on STS tumours utilising a differential attachment approach. Cell lines were characterised by morphology, immunocytochemistry, proliferation assays, short tandem repeat (STR) and microarray-based genomic copy number profiling.
  
  
  RESULTS
  Of 47 STS cases of various subtypes, half formed adherent monolayers. Seven formed self-immortalised cell lines, including three undifferentiated pleomorphic sarcomas, two dedifferentiated liposarcomas (one of which had received radiotherapy), a leiomyosarcoma and a myxofibrosarcoma. Two morphologically distinct yet genetically identical variants were established in separate cultures for the latter two tumours. All cell lines demonstrated genomic and phenotypic features that not only confirm their malignant characteristics but also confirm retention of DNA copy number aberrations present in their parent tumours that likely include drivers.
  
  
  CONCLUSIONS
  These primary cell lines are much-needed additions to the number of reliable cell lines of STS with complex genomics available for initial functional validation of candidate molecular targets.
authors: Department of Oncology and Metabolism, The University of Sheffield, Medical School, Beech Hill Road, Sheffield S10 2RX, UK.
authors: Abdulazeez
authors: A
authors: Salawu
authors: Department of Histopathology, Sheffield Teaching Hospitals, Royal Hallamshire Hospital, Glossop Road Sheffield S10 2JF, UK.
authors: Malee
authors: M
authors: Fernando
authors: Department of Histopathology, Sheffield Teaching Hospitals, Royal Hallamshire Hospital, Glossop Road Sheffield S10 2JF, UK.
authors: David
authors: D
authors: Hughes
authors: Brighton and Sussex Medical School, University of Sussex, Falmer, Brighton BN1 9PX, UK.
authors: Malcolm W R
authors: MW
authors: Reed
authors: Academic Unit of Clinical Oncology, Weston Park Hospital, University of Sheffield, Whitham Road, Sheffield S10 2SJ, UK.
authors: Penella
authors: P
authors: Woll
authors: Department of Oncology and Metabolism, The University of Sheffield, Medical School, Beech Hill Road, Sheffield S10 2RX, UK.
authors: Claire
authors: C
authors: Greaves
authors: Department of Oncology and Metabolism, The University of Sheffield, Medical School, Beech Hill Road, Sheffield S10 2RX, UK.
authors: Chris
authors: C
authors: Day
authors: Department of Oncology and Metabolism, The University of Sheffield, Medical School, Beech Hill Road, Sheffield S10 2RX, UK.|Prince Sultan Military Medical City, Post Box 7897 Riyadh 11159, Kingdom of Saudi Arabia.
authors: Meshal
authors: M
authors: Alhajimohammed
authors: Department of Oncology and Metabolism, The University of Sheffield, Medical School, Beech Hill Road, Sheffield S10 2RX, UK.
authors: Karen
authors: K
authors: Sisley
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D007890:Leiomyosarcoma / Q000235:genetics / Q000473:pathology; D008080:Liposarcoma / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D061251:Primary Cell Culture / Q000379:methods; D012509:Sarcoma / Q000235:genetics* / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000235:genetics* / Q000473:pathology*