title: Fervidobacterium thailandense sp. nov., an extremely thermophilic bacterium isolated from a hot spring.
abstract: Strain FC2004T, a strictly anaerobic, extremely thermophilic heterotroph, was isolated from a hot spring in Thailand. Typical cells of strain FC2004T were rod shaped (0.5-0.6×1.1-2.5 µm) with an outer membrane swelling out over an end. Filaments (10-30 µm long) and membrane-bound spheroids containing two or more cells inside (3-8 µm in diameter) were observed. The temperature range for growth was 60-88°C (optimum 78-80°C), pH range was 6.5-8.5 (optimum pH 7.5) and NaCl concentration range was 0 to <5 g l-1 (optimum 0.5 g l-1). S0 stimulated growth yield. S2O32- and NO3- did not influence growth. Glucose, maltose, sucrose, fructose, cellobiose, CM-cellulose and starch were utilized for growth. The membrane was composed mainly of the saturated fatty acids C16:0 and C18:0. The DNA G+C content was 45.8 mol%. The 16S rRNA gene sequence of strain FC2004T revealed highest similarity to species of the genus Fervidobacterium: F. pennivorans DSM 9078T (97-96 %), F. islandicum AW-1 (96 %), F. changbaicum CBS-1T (96 %), F. islandicum H21T (95 %), F. nodosum Rt17-B1T (95 %), F. riparium 1445tT (95 %) and F. gondwanense AB39T (93 %). Phylogenetic analysis of 16S rRNA gene sequences and average nucleotide identity analysis suggested that strain FC2004T represented a novel species within the genus Fervidobacterium, for which the name Fervidobacterium thailandense sp. nov. is proposed. The type strain is FC2004T (=JCM 18757T=ATCC BAA-2483T).
authors: Department of Microbiology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand.|Department of Mechanical Engineering, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon-Pathom, 73000, Thailand.
authors: Wirojne
authors: W
authors: Kanoksilapatham
authors: Department of Microbiology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand.
authors: Patlada
authors: P
authors: Pasomsup
authors: Department of Microbiology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand.|Western University Kanchanaburi, Huai Krachao District, Kanchanaburi 71170, Thailand.
authors: Porranee
authors: P
authors: Keawram
authors: IRNAS-CSIC, Department of Biogeochemistry, Plant and Microbial Ecology, Avda. Reina Mercedes 10, 41012 Sevilla, Spain.
authors: Alba
authors: A
authors: Cuecas
authors: IRNAS-CSIC, Department of Biogeochemistry, Plant and Microbial Ecology, Avda. Reina Mercedes 10, 41012 Sevilla, Spain.|Departamento Bioquímica y Biotechnología, Universidad Rovira y Virgili, Marceli Domingo 1, Tarragona 43007, Spain.
authors: Maria Carmen
authors: MC
authors: Portillo
authors: IRNAS-CSIC, Department of Biogeochemistry, Plant and Microbial Ecology, Avda. Reina Mercedes 10, 41012 Sevilla, Spain.
authors: Juan M
authors: JM
authors: Gonzalez
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology; D045482:Hot Springs / Q000382:microbiology*; D006358:Hot Temperature; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S