title: Long non-coding RNA MALAT-1 modulates metastatic potential of tongue squamous cell carcinomas partially through the regulation of small proline rich proteins.
abstract: We previously described several abnormally expressed long non-coding RNA (lncRNA) in tong squamous cell carcinomas (TSCCs) that might be associated with tumor progression. In the present study, we aimed to investigate the role of abnormally expressed metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) lncRNA in the metastatic potential of TSCC cells and its molecular mechanisms.
  
  
  
  Expression levels of MALAT-1 lncRNA were examined via quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) in 127 TSCC samples as well as paired adjacent normal tissues and lymph node metastases (if exist). Lentiviral vectors expressing short hairpin RNA (shRNA) were used to knock down the expression of MALAT1 gene in two TSCC cell lines (CAL27 and SCC-25) with relatively higher MALAT-1 expression. Proliferational ability of the TSCC cells was analyzed using water soluble tetrazolium-1 (WST-1) assay. Metastatic abilities of TSCC cells were estimated in-vitro and in-vivo. We also performed a microarray-based screen to identify the genes influenced by MALAT-1 alteration, which were validated by real-time PCR analysis.
  
  
  
  Expression of MALAT-1 lncRNA was enhanced in TSCCs, especially in those with lymph node metastasis (LNM). Knockdown (KD) of MALAT-1 lncRNA in TSCC cells led to impaired migration and proliferation ability in-vitro and fewer metastases in-vivo. DNA microarray analysis showed that several members of small proline rich proteins (SPRR) were up-regulated by KD of MALAT-1 lncRNA in TSCC cells. SPRR2A over-expression could impair distant metastasis of TSCC cells in-vivo.
  
  
  
  Enhanced expression of MALAT-1 is associated with the growth and metastatic potential of TSCCs. Knock down of MALAT-1 in TSCCs leads to the up-regulation of certain SPRR proteins, which influenced the distant metastasis of TSCC cells.
authors: Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong province, China.|Department of Oral and Maxillofacial, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, People's Republic of China.
authors: Zhengyu
authors: Z
authors: Fang
authors: Department of Oral and Maxillofacial, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, People's Republic of China.
authors: Shanshan
authors: S
authors: Zhang
authors: Department of Oral and Maxillofacial, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, People's Republic of China.
authors: Yufan
authors: Y
authors: Wang
authors: Department of Oral and Maxillofacial, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, People's Republic of China.
authors: Shiyue
authors: S
authors: Shen
authors: Department of Oral and Maxillofacial, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, People's Republic of China.
authors: Feng
authors: F
authors: Wang
authors: Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong province, China.
authors: Yinghua
authors: Y
authors: Hao
authors: Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong province, China.
authors: Yuxia
authors: Y
authors: Li
authors: Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong province, China.
authors: Bingyue
authors: B
authors: Zhang
authors: Biomedical Research Institute, Shenzhen Peking University- The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong province, China.
authors: You
authors: Y
authors: Zhou
authors: Department of Oral and Maxillofacial, Shenzhen Hospital, Peking University, Shenzhen, Guangdong Province, People's Republic of China. hy192@tom.com.
authors: Hongyu
authors: H
authors: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000473:pathology*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D000077195:Squamous Cell Carcinoma of Head and Neck; D014062:Tongue Neoplasms / Q000235:genetics / Q000473:pathology*; D055815:Young Adult
chemical_list: C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding
keywords: Cancer metastasis; Long non-coding RNA; MALAT-1; Tongue squamous cell cancer