title: New Findings on the Mechanism of Perspiration Including Aquaporin-5 Water Channel.
abstract: Aquaporin-5 (AQP5) is a member of the water channel protein family. Although AQP5 has been shown to be present in sweat glands, the presence or absence of regulated intracellular translocation of AQP5 in sweat glands remains to be determined. In this article, recent findings on AQP5 in sweat glands are presented. (1) Immunoreactive AQP5 was detected in the apical membranes and the intercellular canaliculi of secretory coils, and in the basolateral membranes of the clear cells in human eccrine sweat glands. (2) AQP5 rapidly concentrated at the apical membranes during sweating in mouse sweat glands. (3) Treatment of human AQP5-expressing Madin-Darby canine kidney cells with calcium ionophore A23187 resulted in a twofold increase in the AQP5 level in the apical membranes within 5 min. (4) Anoctamin-1, a calcium-activated chloride channel was detected in the apical membranes and it completely colocalized with AQP5 in the apical membranes in mouse sweat glands. AQP5 may be involved in sweating and its translocation may help to increase the water permeability of the apical membranes of sweat glands. AQP5 is a potential target molecule for the design of a sweat-modulating drug.
authors: Department of Dermatology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Risako
authors: R
authors: Inoue
mesh_terms: D000818:Animals; D000075369:Anoctamin-1; D051402:Aquaporin 5 / Q000187:drug effects / Q000502:physiology*; D001692:Biological Transport; D000001:Calcimycin / Q000494:pharmacology; D061207:Calcium Ionophores / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D018118:Chloride Channels / Q000378:metabolism; D004285:Dogs; D006801:Humans; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D013545:Sweat Glands / Q000378:metabolism / Q000502:physiology*; D013546:Sweating / Q000502:physiology*
chemical_list: C531181:ANO1 protein, mouse; D000075369:Anoctamin-1; D051402:Aquaporin 5; D061207:Calcium Ionophores; D018118:Chloride Channels; D000001:Calcimycin