title: miRNA expression profiling of 'noninvasive follicular thyroid neoplasms with papillary-like nuclear features' compared with adenomas and infiltrative follicular variants of papillary thyroid carcinomas.
abstract: Follicular variants of papillary thyroid carcinoma include encapsulated (with or without capsular/vascular invasion) and infiltrative forms, which have different clinical behaviors. The encapsulated forms that lack capsular invasion have an indolent clinical behavior that is similar to benign lesions; therefore, they were recently reclassified as 'noninvasive follicular thyroid neoplasms with papillary-like nuclear features' (NIFTPs). Because NIFTPs have nuclear features of papillary carcinomas, distinguishing between NIFTPs and infiltrative follicular variant of papillary thyroid carcinoma is almost impossible with cytological examination. The aim of this study is to determine whether miRNA expression profiles may help distinguish between NIFTPs versus follicular adenomas and infiltrative follicular variant of papillary thyroid carcinomas. The expression profiling of 798 miRNAs was tested in 54 thyroid tumors, including 18 follicular adenomas, 19 NIFTPs and 17 infiltrative follicular variant of papillary thyroid carcinomas, using nCounter Nanostring. We found that miR-146-5p, miR-221-5p, miR-222-3p, miR-30e-3p, and miR-152-3p could discriminate between benign and malignant lesions with a very high level of significance (P-value<0.001). High expression levels of miR-146-5p, miR-199a-5p, miR-199b-5p, miR-1285-5p, miR-1915-3p, and miR-4516, and low miR-148b-3p expression were associated with infiltrative growth of follicular variant of papillary thyroid carcinomas. Interestingly, miR-152-3p, miR-185-5p, and miR-574-3p were significantly downregulated in NIFTPs compared with follicular adenomas, whereas miR-10a-5p and miR-320e can discriminate between NIFTPs and infiltrative forms of follicular variant of papillary thyroid carcinomas. In conclusion, a panel of these markers could have high diagnostic potential as well as could be applied to presurgical fine-needle aspiration, especially for lesions classified as indeterminate thyroid nodules.
authors: Department of Surgical Pathology, Medical, Molecular and Critical Area, University of Pisa, Pisa, Italy.
authors: Nicla
authors: N
authors: Borrelli
authors: Department of Surgical Pathology, Medical, Molecular and Critical Area, University of Pisa, Pisa, Italy.
authors: Maria
authors: M
authors: Denaro
authors: Department of Laboratory Medicine, Section of Pathology Azienda Ospedaliero-Universitaria Pisana, Pisa, Italy.
authors: Clara
authors: C
authors: Ugolini
authors: Department of Surgical Pathology, Medical, Molecular and Critical Area, University of Pisa, Pisa, Italy.
authors: Anello Marcello
authors: AM
authors: Poma
authors: Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
authors: Mario
authors: M
authors: Miccoli
authors: Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
authors: Paolo
authors: P
authors: Vitti
authors: Department of Surgical Pathology, Medical, Molecular and Critical Area, University of Pisa, Pisa, Italy.
authors: Paolo
authors: P
authors: Miccoli
authors: Department of Surgical Pathology, Medical, Molecular and Critical Area, University of Pisa, Pisa, Italy.
authors: Fulvio
authors: F
authors: Basolo
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000236:Adenoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014408:Biomarkers, Tumor; D018265:Carcinoma, Papillary, Follicular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs