title: The comparison of structure and anticancer activity in vitro of polysaccharides from brown algae Alaria marginata and A. angusta.
abstract: Laminaran and three fucoidan fractions were obtained from the brown alga Alaria marginata. Alaria angusta, studied earlier by us, has the same polysaccharide composition. Galactofucan AmF3 from A. marginata has a main chain of →3)-α-l-Fucp-(2,4-SO3(-))-(1→residues, similar to galactofucan from A. angusta. However, the structure of the branches in fucoidan AmF3 can differ from those in the fucoidan from A. angusta. The following fragments were identified in AmF3: HexA-(1→2)-Fuc, HexA-(1→2)-Gal, Gal-(1→4)-HexA, Fuc-(1→2)-Gal-6-SO3(-), Fuc-4-SO3(-)-(1→6)-Gal, Gal-(1→2)-Gal-2-SO3(-), Gal-4-SO3(-)-(1 →6)-Gal, Gal-4-SO3(-)-(1→3)-Fuc-(1→3)-Fuc, Fuc-4-SO3(-)-(1→6)-Gal-(1→4)-Gal, Gal-(1→4)-Gal-(1→3)-Fuc, Gal-2-SO3(-)-(1→4)-Gal-(1→4)-Gal, Gal-(1→4)-Gal-6-SO3(-)-(1→2)-Gal. Chains of galactose residues (DP up to 9) were found in AmF3 fucoidan. The laminarans, galactofucans and their derivatives from both algae exhibited no cytotoxicity in vitro. Polysaccharides from A. angusta were more effective against colony formation of HT-29 cells, while those from A. marginata had a greater effect on T-47D cells. Sulfated and desulfated fucoidans possessed weak antitumor activity using SK-MEL-28 cells.
authors: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku prosp., 159, 690022, Vladivostok, Russian Federation. Electronic address: Usoltseva-R@yandex.ru.
authors: Roza V
authors: RV
authors: Usoltseva Menshova
authors: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku prosp., 159, 690022, Vladivostok, Russian Federation.
authors: Stanislav D
authors: SD
authors: Anastyuk
authors: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku prosp., 159, 690022, Vladivostok, Russian Federation.
authors: Natalia M
authors: NM
authors: Shevchenko
authors: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku prosp., 159, 690022, Vladivostok, Russian Federation.
authors: Tatiana N
authors: TN
authors: Zvyagintseva
authors: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku prosp., 159, 690022, Vladivostok, Russian Federation.
authors: Svetlana P
authors: SP
authors: Ermakova
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D019073:HT29 Cells; D006801:Humans; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014410:Tumor Stem Cell Assay
chemical_list: D000970:Antineoplastic Agents; D005936:Glucans; D011134:Polysaccharides; C008247:laminaran; C007789:fucoidan
keywords: Anticancer activity; Brown alga; Depolymerization; Fucoidan; Structure