title: Activation and selective IL-17 response of human Vγ9Vδ2 T lymphocytes by TLR-activated plasmacytoid dendritic cells.
abstract: Vγ9Vδ2 T cells and plasmacytoid dendritic cells (pDCs) are two distinct cell types of innate immunity that participate in early phases of immune response. We investigated whether a close functional relationship exists between these two cell populations using an in vitro co-culture in a human system.pDCs that had been activated by IL-3 and the TLR9 ligand CpG induced substantial activation of Vγ9Vδ2 T cells upon co-culture, which was cell-to-cell contact dependent, as demonstrated in transwell experiments, but that did not involve any of the costimulatory molecules potentially expressed by pDCs or Vγ9V2 T cells, such as ICOS-L, OX40 and CD40L. Activated pDCs selectively induced IL-17, but not IFN-γ, responses of Vγ9Vδ2T cells, which was dominant over the antigen-induced response, and this was associated with the expansion of memory (both central and effector memory) subsets of Vγ9Vδ2 T cells.Overall, our results provide a further piece of information on the complex relationship between these two populations of cells with innate immunity features during inflammatory responses.
authors: Central Laboratory of Advanced Diagnosis and Biomedical Research (CLADIBIOR), University of Palermo, Palermo, Italy.|Department of Biopathology and Medical Biotechnologies (DIBIMED), University of Palermo, Palermo, Italy.
authors: Elena
authors: E
authors: Lo Presti
authors: Central Laboratory of Advanced Diagnosis and Biomedical Research (CLADIBIOR), University of Palermo, Palermo, Italy.|Department of Biopathology and Medical Biotechnologies (DIBIMED), University of Palermo, Palermo, Italy.
authors: Nadia
authors: N
authors: Caccamo
authors: Central Laboratory of Advanced Diagnosis and Biomedical Research (CLADIBIOR), University of Palermo, Palermo, Italy.|Department of Biopathology and Medical Biotechnologies (DIBIMED), University of Palermo, Palermo, Italy.
authors: Valentina
authors: V
authors: Orlando
authors: Central Laboratory of Advanced Diagnosis and Biomedical Research (CLADIBIOR), University of Palermo, Palermo, Italy.|Department of Biopathology and Medical Biotechnologies (DIBIMED), University of Palermo, Palermo, Italy.
authors: Francesco
authors: F
authors: Dieli
authors: Central Laboratory of Advanced Diagnosis and Biomedical Research (CLADIBIOR), University of Palermo, Palermo, Italy.|Department of Biopathology and Medical Biotechnologies (DIBIMED), University of Palermo, Palermo, Italy.
authors: Serena
authors: S
authors: Meraviglia
mesh_terms: D017951:Antigen Presentation; D023201:CD40 Ligand / Q000378:metabolism; D002450:Cell Communication; D049109:Cell Proliferation; D018920:Coculture Techniques; D018899:CpG Islands; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D007156:Immunologic Memory; D060945:Inducible T-Cell Co-Stimulator Ligand / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008024:Ligands; D008213:Lymphocyte Activation / Q000276:immunology*; D010641:Phenotype; D053262:Receptors, OX40 / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: C498514:ICOSLG protein, human; C508717:IL17A protein, human; D060945:Inducible T-Cell Co-Stimulator Ligand; D020381:Interleukin-17; D008024:Ligands; D053262:Receptors, OX40; C505716:TNFRSF4 protein, human; D023201:CD40 Ligand; D007371:Interferon-gamma
keywords: IL-17; Immune response; Immunity; Immunology and Microbiology Section; TLR activation; plasmacytoid dendritic cells; proliferation; γδ T cells