title: Utility of different serum fibrosis markers in diagnosing patients with chronic pancreatitis and pancreatic adenocarcinoma.
abstract: OBJECTIVE
  To estimate the levels of serum cytokines in chronic pancreatitis (CP) and pancreatic ductal adenocarcinoma (PDAC) patients in order to evaluate their usefulness as possible biomarkers.
  
  
  METHODS
  The study included 167 Caucasian patients: 74 with PDAC (28 men and 42 women, aged 30-88 years), 78 with CP (50 men and 21 women, aged 20-79 years) and 15 age-matched healthy controls hospitalized in the Department of Digestive Tract Diseases, Medical University of Lodz, Poland between 2006 and 2013. Serum MCP-1, transforming growth factor (TGF)-β1, HA and s-Fr were measured in patients with CP (n = 78), PDAC (n = 74) and healthy controls (n = 15) using ELISA (Corgenix United Kingdom Ltd R and D Systems). The severity of CP was assessed according to the Cambridge classification.
  
  
  RESULTS
  Both patients with CP and PDAC had a significantly higher mean TGF-β1 serum level (1066 ± 582 and 888 ± 356 vs 264 ± 93, P < 0.0001), mean s-Fr (2.42 ± 1.385 and 2.41 ± 1.275 vs 0.6 ± 0.370, P < 0.0001) and mean HA (199 ± 254 and 270 ± 358 vs 40 ± 26, P < 0.0001) compared to controls. There was no difference in mean MCP-1 between all the groups. There were no significant differences in any cytokine levels between the PC and PDAC groups. No significant differences between serum cytokines depending on age, gender or smoking status were found in CP patients. Mean s-Fr concentration was significantly higher in CP, lasting longer than 5 years compared to those with a shorter disease clinical course (2.639 ± 1.125 vs 1.870 ± 0.970, P < 0.03). There was no correlation between tumor size, localization or TNM classification and serum TGF-β1, MCP-1, s-Fr and HA levels in patients with PDAC. No significant differences between cytokines depending on diabetes presence in CP were found. Nevertheless, mean serum TGF-β1 concentration in PDAC patients was higher in those with diabetes compared to the remaining group (986 vs 839, P = 0.043).
  
  
  CONCLUSIONS
  Serum TGF-β1, s-Fr and HA may be considered additional diagnostic markers of CP and PDAC. TGF-β1 may be useful to predict endocrine insufficiency in PDAC.
authors: Anna Kozak, Renata Talar-Wojnarowska, Aleksandra Kaczka, Ewa Małecka-Panas, Anita Gąsiorowska, Department of Digestive Tract Diseases, Medical University of Lodz, 90-153 Lodz, Poland.
authors: Anna
authors: A
authors: Kozak
authors: Anna Kozak, Renata Talar-Wojnarowska, Aleksandra Kaczka, Ewa Małecka-Panas, Anita Gąsiorowska, Department of Digestive Tract Diseases, Medical University of Lodz, 90-153 Lodz, Poland.
authors: Renata
authors: R
authors: Talar-Wojnarowska
authors: Anna Kozak, Renata Talar-Wojnarowska, Aleksandra Kaczka, Ewa Małecka-Panas, Anita Gąsiorowska, Department of Digestive Tract Diseases, Medical University of Lodz, 90-153 Lodz, Poland.
authors: Aleksandra
authors: A
authors: Kaczka
authors: Anna Kozak, Renata Talar-Wojnarowska, Aleksandra Kaczka, Ewa Małecka-Panas, Anita Gąsiorowska, Department of Digestive Tract Diseases, Medical University of Lodz, 90-153 Lodz, Poland.
authors: Anna
authors: A
authors: Borkowska
authors: Anna Kozak, Renata Talar-Wojnarowska, Aleksandra Kaczka, Ewa Małecka-Panas, Anita Gąsiorowska, Department of Digestive Tract Diseases, Medical University of Lodz, 90-153 Lodz, Poland.
authors: Leszek
authors: L
authors: Czupryniak
authors: Anna Kozak, Renata Talar-Wojnarowska, Aleksandra Kaczka, Ewa Małecka-Panas, Anita Gąsiorowska, Department of Digestive Tract Diseases, Medical University of Lodz, 90-153 Lodz, Poland.
authors: Ewa
authors: E
authors: Małecka-Panas
authors: Anna Kozak, Renata Talar-Wojnarowska, Aleksandra Kaczka, Ewa Małecka-Panas, Anita Gąsiorowska, Department of Digestive Tract Diseases, Medical University of Lodz, 90-153 Lodz, Poland.
authors: Anita
authors: A
authors: Gąsiorowska
keywords: Chronic pancreatitis; Fibrosis; Hyaluronic acid; Monocyte chemoattractant protein-1; Pancreatic adenocarcinoma; Soluble type fractalkine; Transforming growth factor beta-1