title: The cytotoxic T cells may contribute to the in situ immune response in Jorge Lobo's Disease human lesions.
abstract: Jorge Lobo's Disease (JLD) is a cutaneous chronic granulomatous disease caused by the pathogenic fungus Lacazia loboi. It is characterized by a granulomatous reaction with multinucleated giant cells and high number of fungal cells. In order to contribute to the comprehension of immune mechanisms in JLD human lesions, we studied the cytotoxic immune response, focusing on TCD8+ and NK cells, and granzyme B. Forty skin biopsies of lower limbs were selected and an immunohistochemistry protocol was developed to detect CD8+ T cells, NK cells and Granzyme B. In order to compare the cellular populations, we also performed a protocol to visualize TCD4+ cells. Immunolabeled cells were quantified in nine randomized fields in the dermis. Lesions were characterized by inflammatory infiltrate of macrophages, lymphocytes, epithelioid and multinucleated giant cells with intense number of fungal forms. There was a prevalence of CD8 over CD4 cells, followed by NK cells. Our results suggest that in JLD the cytotoxic immune response could represent another important mechanism to control Lacazia loboi infection. We may suggest that, although CD4+ T cells are essential for host defense in JLD, CD8+ T cells could play a role in the elimination of the fungus.
authors: Faculdade de Medicina, Universidade de São Paulo, Departamento de Patologia.|Programa de Pós-graduação em Ciências da Saúde/Instituto de Assistência Médica ao Servidor Público Estadual.
authors: Ariane Fernandes
authors: AF
authors: Alexandre
authors: Núcleo de Medicina Tropical, Universidade Federal do Pará.|Universidade do Estado do Pará.
authors: Juarez Antonio Simões
authors: JA
authors: Quaresma
authors: Faculdade de Medicina, Universidade de São Paulo, Departamento de Patologia.|Programa de Pós-graduação em Ciências da Saúde/Instituto de Assistência Médica ao Servidor Público Estadual.
authors: Tânia Cristina
authors: TC
authors: Barboza
authors: Núcleo de Medicina Tropical, Universidade Federal do Pará.
authors: Arival Cardoso
authors: AC
authors: de Brito
authors: Núcleo de Medicina Tropical, Universidade Federal do Pará.
authors: Marília Brasil
authors: MB
authors: Xavier
authors: Núcleo de Medicina Tropical, Universidade Federal do Pará.
authors: Clivia Maria Moraes
authors: CM
authors: de Oliveira
authors: Núcleo de Medicina Tropical, Universidade Federal do Pará.
authors: Deborah Aben Athar
authors: DA
authors: Unger
authors: Faculdade de Medicina, Universidade de São Paulo, Departamento de Patologia.
authors: Luciane
authors: L
authors: Kanashiro-Galo
authors: Faculdade de Medicina, Universidade de São Paulo, Departamento de Patologia.
authors: Mirian Nacagami
authors: MN
authors: Sotto
authors: Faculdade de Medicina, Universidade de São Paulo, Departamento de Patologia.
authors: Maria Irma Seixas
authors: MI
authors: Duarte
authors: Faculdade de Medicina, Universidade de São Paulo, Departamento de Patologia cpagliari@usp.br.|Programa de Pós-graduação em Ciências da Saúde/Instituto de Assistência Médica ao Servidor Público Estadual.
authors: Carla
authors: C
authors: Pagliari
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D015726:Giant Cells / Q000276:immunology; D053804:Granzymes / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007694:Killer Cells, Natural / Q000276:immunology; D060367:Lacazia / Q000254:growth & development / Q000276:immunology*; D060368:Lobomycosis / Q000473:pathology*; D008264:Macrophages / Q000276:immunology; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D012867:Skin / Q000473:pathology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D053804:Granzymes
keywords: Jorge Lobo's disease; cytotoxic response; human lesions; immunohistochemistry; skin