title: MicroRNA expression profiles in testicular biopsies of patients with impaired spermatogenesis.
abstract: Spermatogenesis is a complex process that involves thousands of genes whose expression during different stages is strictly regulated. Small non-coding microRNAs play an important role in the posttranscriptional regulation of mRNA processing during spermatogenesis. Using Agilent SurePrint v16 microRNA 8 × 60 K microarray kit, we investigated the microRNA expression profiles of 24 formalin-fixed paraffin-embedded testicular biopsies from patients with hypospermatogenesis (n = 10), hypospermatogenesis and azoospermia factor c region on the Y chromosome (AZFc) deletion (n = 3), Sertoli cell-only syndrome (n = 3) and maturation arrest (n = 2), in comparison with subjects with normal spermatogenesis (n = 6). After adjusting for multiple testing, six deregulated miRNAs were detected in the patients with AZFc deletion, 30 in maturation arrest group, 52 in Sertoli cell-only syndrome group of patients, and none in the group of patients with hypospermatogenesis. Some of the deregulated microRNAs were shared between groups, resulting in 58 unique differentially expressed microRNAs. The expression of five microRNAs (hsa-miR-34b, hsa-miR-449b, hsa-miR-517c, hsa-miR-181c, and hsa-miR-605) was validated by qRT-PCR in a total of 74 samples. Using mRNA expression profiles of subjects with matching histopathological patterns of impaired spermatogenesis from publically available Gene Expression Omnibus data sets, we have performed integrated mRNA-microRNA regulatory network analysis. Pathway analysis revealed significantly enriched set of genes for tumor necrosis factor-related apoptosis-inducing ligand signaling pathway, previously shown to be involved in regulation of apoptosis in normal functioning testis. Our results should be considered as preliminary as we have analyzed only a small number of patients in each studied group. Further studies with larger number of patients with impaired spermatogenesis as well as more targeted approaches with parallel microRNA and mRNA expression profiling in isolated subpopulations of somatic or germ cells from different stages of spermatogenesis are needed to clarify the role of the microRNAs in the process of spermatogenesis.
authors: Research Center for Genetic Engineering and Biotechnology 'Georgi D. Efremov', Macedonian Academy of Science and Arts, Skopje, Republic of Macedonia.
authors: P
authors: P
authors: Noveski
authors: Research Center for Genetic Engineering and Biotechnology 'Georgi D. Efremov', Macedonian Academy of Science and Arts, Skopje, Republic of Macedonia.
authors: K
authors: K
authors: Popovska-Jankovic
authors: Clinical Hospital 'Acibadem Sistina', Skopje, Republic of Macedonia.
authors: K
authors: K
authors: Kubelka-Sabit
authors: Clinical Hospital 'Acibadem Sistina', Skopje, Republic of Macedonia.
authors: V
authors: V
authors: Filipovski
authors: Clinical Hospital 'Acibadem Sistina', Skopje, Republic of Macedonia.
authors: S
authors: S
authors: Lazarevski
authors: Faculty of Medicine, Clinic of Endocrinology and Metabolic Disorders, Skopje, Republic of Macedonia.
authors: T
authors: T
authors: Plaseski
authors: Research Center for Genetic Engineering and Biotechnology 'Georgi D. Efremov', Macedonian Academy of Science and Arts, Skopje, Republic of Macedonia.
authors: D
authors: D
authors: Plaseska-Karanfilska
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009845:Oligospermia / Q000235:genetics; D054331:Sertoli Cell-Only Syndrome / Q000235:genetics; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000473:pathology
chemical_list: D035683:MicroRNAs
keywords: impaired spermatogenesis; male infertility; microRNA-mRNA; testis miRNA; tumor necrosis factor-related apoptosis-inducing ligand