title: Fabrication and Application of Photocrosslinked, Nanometer-Scale, Physically Adsorbed Films for Tissue Culture Regeneration.
abstract: This study describes the development and cell culture application of nanometer thick photocrosslinkable thermoresponsive polymer films prepared by physical adsorption. Two thermoresponsive polymers, poly(N-isopropylacrylamide (NIPAm)-co-acrylamidebenzophenone (AcBzPh)) and poly(NIPAm-co-AcBzPh-co-N-tertbutylacrylamide) are investigated. Films are prepared both above and below the polymers' lower critical solution temperatures (LCSTs) and cross-linked, to determine the effect, adsorption preparation temperature has on the resultant film. The films prepared at temperatures below the LCST are smoother, thinner, and more hydrophilic than those prepared above. Human pulmonary microvascular endothelial cell (HPMEC) adhesion and proliferation are superior on the films produced below the polymers LCST compared to those produced above. Cells sheets are detached by simply lowering the ambient temperature to below the LCST. Transmission electron, scanning electron, and light microscopies indicate that the detached HPMEC sheets maintain their integrity.
authors: School of Chemistry, National University of Ireland Galway, H91 CF50, Galway, Ireland.
authors: Deirdre
authors: D
authors: Healy
authors: School of Chemistry, National University of Ireland Galway, H91 CF50, Galway, Ireland.
authors: Maria E
authors: ME
authors: Nash
authors: School of Chemistry and Chemical Biology, University College Dublin, D04 R7R0, Belfield, Dublin 4, Ireland.
authors: Alexander
authors: A
authors: Gorelov
authors: Center for Microscopy and Imaging, Anatomy, School of Medicine, National University of Ireland Galway, H91 CF50, Galway, Ireland.
authors: Kerry
authors: K
authors: Thompson
authors: Anatomy, School of Medicine, National University of Ireland Galway, H91 CF50, Galway, Ireland.
authors: Peter
authors: P
authors: Dockery
authors: Materials and Surface Science Institute, University of Limerick, V94 DPY6, Limerick, Ireland.
authors: Sergey
authors: S
authors: Beloshapkin
authors: School of Chemistry, National University of Ireland Galway, H91 CF50, Galway, Ireland.|Sechenov First Moscow State Medical University, Institute for Regenerative Medicine, 119991, Moscow, Russia.
authors: Yury
authors: Y
authors: Rochev
mesh_terms: D016475:3T3 Cells; D000178:Acrylamides / Q000737:chemistry / Q000494:pharmacology; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008027:Light; D008168:Lung / Q000098:blood supply; D008422:Materials Testing; D051379:Mice; D018625:Microscopy, Atomic Force; D055806:Microvessels / Q000166:cytology; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D012038:Regeneration / Q000187:drug effects*; D046509:Tissue Culture Techniques / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D000178:Acrylamides; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D014867:Water; C067295:N-isopropylacrylamide
keywords: adsorption; cross-linking; pNIPAm; thermoresponsive polymer; tissue engineering