title: Plasma heme oxygenase-1 is decreased in peripheral artery disease patients.
abstract: Peripheral artery disease (PAD) is a common manifestation of atherosclerosis. A number of emerging risk factors, including oxidative stress biomarkers, free radicals and heat shock proteins, may add to the established risk factors for cardiovascular disease (CVD). The present study assessed surrogate markers of oxidative stress, including total reduced glutathione (GSH), lipid hydroperoxides (LOOH), isoprostanes, heme oxygenase‑1 (HO‑1) and metabolic biomarkers, such as adiponectin and lactate, in PAD patients (n=27). Healthy age‑matched volunteers (n=27) served as controls. GSH and LOOH were evaluated by measuring total thiol groups and iron oxidation, respectively, by spectrophotometric analysis. Adiponectin, isoprostanes and HO‑1 levels were determined using commercially available ELISA kits and lactate level was determined colorimetrically. Results from patients with PAD demonstrated no significant difference in GSH content and LOOH formation when compared with healthy controls (5.1±7.6 vs. 6.9±9.1 nmol/ml and 6.8±14.2 vs. 8.3±14.9 nmol/ml, respectively), however, isoprostanes were demonstrated to be significantly reduced (3.8±4.8 pg/ml vs. 120±91 pg/mll; P<0.001). Furthermore, HO‑1, a protective heat shock protein, was significantly reduced in PAD patients (0.8±0.7 vs. 3.4±1.3 ng/ml; P<0.001). Adiponectin, an antiatherogenic adipokine, was not significantly different between the two groups (1.4±0.2 vs. 1.5±0.5 µg/ml), whereas serum lactate was significantly higher in PAD patients compared with controls (0.11±0.01 vs. 0.1±0.01 mM; P<0.05). Using multivariate analysis, HO‑1, hypertension, smoking and dyslipidemia were indicated to be independently associated with the presence of PAD, while only ankle‑brachial index was an independent predictor of severity of PAD. The oxidative pathway may be partially involved in the onset and progression of PAD and may represent a target to reduce the risk of ischemic events.
authors: Department of Clinical and Experimental Medicine, University of Catania, I-95100 Catania, Italy.
authors: Salvatore Santo
authors: SS
authors: Signorelli
authors: Department of Biomedical and Biotechnological Science, University of Catania, I-95100 Catania, Italy.
authors: Guido
authors: G
authors: Li Volsi
authors: Department of Clinical and Experimental Medicine, University of Catania, I-95100 Catania, Italy.
authors: Valerio
authors: V
authors: Fiore
authors: Department of Clinical and Experimental Medicine, University of Catania, I-95100 Catania, Italy.
authors: Marco
authors: M
authors: Mangiafico
authors: Department of Drug Science, University of Catania, I-95100 Catania, Italy.
authors: Ignazio
authors: I
authors: Barbagallo
authors: Department of Biomedical and Biotechnological Science, University of Catania, I-95100 Catania, Italy.
authors: Rosalba
authors: R
authors: Parenti
authors: Biomedical Department of Internal Medicine and Medical Specialties, University of Palermo, I-90139 Palermo, Italy.
authors: Manfredi
authors: M
authors: Rizzo
authors: Department of Biomedical and Biotechnological Science, University of Catania, I-95100 Catania, Italy.
authors: Giovanni
authors: G
authors: Li Volti
mesh_terms: D052242:Adiponectin / Q000097:blood / Q000378:metabolism; D000368:Aged; D055109:Ankle Brachial Index; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000097:blood* / Q000378:metabolism; D006801:Humans; D028421:Isoprostanes / Q000097:blood / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D058729:Peripheral Arterial Disease / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism
chemical_list: D052242:Adiponectin; D015415:Biomarkers; D028421:Isoprostanes; D051547:Heme Oxygenase-1; D005978:Glutathione