title: DNA binding, DNA cleavage and HSA interaction of several metal complexes containing N-(2-hydroxyethyl)-N'-benzoylthiourea and 1,10-phenanthroline ligands.
abstract: Four novel ternary metal complexes of the type [M(Phen)(L1)2)] [phen = 1,10-phenanthroline, L1 = N-(2-hydroxyethyl)-N'-benzoylthiourea, M = Ni(II)(1), Co(II) (2), Cu(II) (3), Pd(II) (4)] were synthesized. The organic ligands and their corresponding organometallic complexes have been characterized using UV-vis absorption spectroscopy, element analysis, infrared radiation spectroscopy and fluorescence spectra. DNA binding and cleavage studies of these complexes were conducted in detail. In vitro DNA-binding properties were studied by electronic absorption spectra and fluorescence spectra methods. The results indicate that all of the ternary metal complexes can efficiently bind to DNA via intercalation mode. The DNA-binding constants for all ternary compounds are around 4 × 10(6) M(-1). The binding propensity of the complexes to human serum albumin (HSA) was also investigated. Agarose gel electrophoresis study revealed that the metal complexes could cleave super-coiled pBR322 DNA to a nicked form in the absence of external agents. In vitro anti bacterial studies show that copper complex has weak antibacterial activities. Copper complex exhibits a better biological activity among all complexes. This study provides a new perspective and evaluation on the role and importance of the effect factors on the medicinal properties of benzoylthiourea compounds. Synchronous fluorescence spectra of HSA (10 μM) as a function of concentration of the complexes 1-4.
authors: Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, Key Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China. pengbo.nwnu@hotmail.com.
authors: Bo
authors: B
authors: Peng
authors: Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, Key Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China.
authors: Zhuantao
authors: Z
authors: Gao
authors: College of Life Science, Northwest Normal University, Lanzhou, 730070, China.
authors: Xibo
authors: X
authors: Li
authors: Food Test Center of Jiuquan, Jiuquan, 735000, China.
authors: Tingting
authors: T
authors: Li
authors: Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, Key Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China.
authors: Guorong
authors: G
authors: Chen
authors: Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, Key Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China.
authors: Min
authors: M
authors: Zhou
authors: College of Life Science, Northwest Normal University, Lanzhou, 730070, China.
authors: Ji
authors: J
authors: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D010618:Phenanthrolines / Q000737:chemistry*; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000187:drug effects; D013890:Thiourea / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D010618:Phenanthrolines; D012709:Serum Albumin; C474261:N-benzoylthiourea; D004247:DNA; D013890:Thiourea; C025205:1,10-phenanthroline
keywords: Antibacterial activities; Complex; DNA; Phenanthroline; Thiourea