title: miR-378 attenuates muscle regeneration by delaying satellite cell activation and differentiation in mice.
abstract: Skeletal muscle mass and homeostasis during postnatal muscle development and regeneration largely depend on adult muscle stem cells (satellite cells). We recently showed that global overexpression of miR-378 significantly reduced skeletal muscle mass in mice. In the current study, we used miR-378 transgenic (Tg) mice to assess the in vivo functional effects of miR-378 on skeletal muscle growth and regeneration. Cross-sectional analysis of skeletal muscle tissues showed that the number and size of myofibers were significantly lower in miR-378 Tg mice than in wild-type mice. Attenuated cardiotoxin-induced muscle regeneration in miR-378 Tg mice was found to be associated with delayed satellite cell activation and differentiation. Mechanistically, miR-378 was found to directly target Igf1r in muscle cells both in vitro and in vivo These miR-378 Tg mice may provide a model for investigating the physiological and pathological roles of skeletal muscle in muscle-associated diseases in humans, particularly in sarcopenia.
authors: Division of Geriatrics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences , Beijing 100730, China.
authors: Ping
authors: P
authors: Zeng
authors: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College , Beijing 100005, China.
authors: Wanhong
authors: W
authors: Han
authors: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College , Beijing 100005, China.
authors: Changyin
authors: C
authors: Li
authors: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College , Beijing 100005, China.
authors: Hu
authors: H
authors: Li
authors: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College , Beijing 100005, China.
authors: Dahai
authors: D
authors: Zhu
authors: The State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College , Beijing 100005, China xhliu41@medmail.com.cn yongzhang@ibms.pumc.edu.cn.
authors: Yong
authors: Y
authors: Zhang
authors: Division of Geriatrics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences , Beijing 100730, China xhliu41@medmail.com.cn yongzhang@ibms.pumc.edu.cn.
authors: Xiaohong
authors: X
authors: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics*; D018482:Muscle, Skeletal / Q000502:physiology; D017526:Receptor, IGF Type 1 / Q000235:genetics / Q000502:physiology; D012038:Regeneration / Q000235:genetics* / Q000502:physiology*; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000502:physiology*
chemical_list: C546119:MIRN378 microRNA, mouse; D035683:MicroRNAs; D017526:Receptor, IGF Type 1
keywords: Igf1r; differentiation; miR-378; muscle regeneration; satellite cell activation