title: Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein-Ligand Interactions.
abstract: A highly efficient cap-exchange approach for preparing compact, dense polyvalent mannose-capped quantum dots (QDs) has been developed. The resulting QDs have been successfully used to probe multivalent interactions of HIV/Ebola receptors DC-SIGN and DC-SIGNR (collectively termed as DC-SIGN/R) using a sensitive, ratiometric Förster resonance energy transfer (FRET) assay. The QD probes specifically bind DC-SIGN, but not its closely related receptor DC-SIGNR, which is further confirmed by its specific blocking of DC-SIGN engagement with the Ebola virus glycoprotein. Tuning the QD surface mannose valency reveals that DC-SIGN binds more efficiently to densely packed mannosides. A FRET-based thermodynamic study reveals that the binding is enthalpy-driven. This work establishes QD FRET as a rapid, sensitive technique for probing structure and thermodynamics of multivalent protein-ligand interactions.
authors: School of Chemistry and Astbury Centre for Structural Molecular Biology University of Leeds Leeds LS2 9JT UK.
authors: Yuan
authors: 0000-0003-4607-7356
authors: Y
authors: Guo
authors: School of Chemistry and Astbury Centre for Structural Molecular Biology University of Leeds Leeds LS2 9JT UK.
authors: Chadamas
authors: C
authors: Sakonsinsiri
authors: Infection Biology Unit German Primate Center Kellnerweg 4 37077 Göttingen Germany.
authors: Inga
authors: I
authors: Nehlmeier
authors: Department of Chemistry University of York Heslington York YO10 5DD UK.
authors: Martin A
authors: MA
authors: Fascione
authors: School of Chemistry and Astbury Centre for Structural Molecular Biology University of Leeds Leeds LS2 9JT UK.
authors: Haiyan
authors: H
authors: Zhang
authors: School of Chemistry and Astbury Centre for Structural Molecular Biology University of Leeds Leeds LS2 9JT UK.
authors: Weili
authors: W
authors: Wang
authors: Infection Biology Unit German Primate Center Kellnerweg 4 37077 Göttingen Germany.
authors: Stefan
authors: S
authors: Pöhlmann
authors: School of Chemistry and Astbury Centre for Structural Molecular Biology University of Leeds Leeds LS2 9JT UK.
authors: W Bruce
authors: 0000-0002-7352-0360
authors: WB
authors: Turnbull
authors: School of Chemistry and Astbury Centre for Structural Molecular Biology University of Leeds Leeds LS2 9JT UK.
authors: Dejian
authors: 0000-0003-3314-9242
authors: D
authors: Zhou
keywords: FRET; Kohlenhydrate; Proteine; Quantenpunkte; Virenhemmung