title: Hydrostatic Pressure Increases the Catalytic Activity of Amyloid Fibril Enzymes.
abstract: We studied the combined effects of pressure (0.1-200 MPa) and temperature (22, 30, and 38 °C) on the catalytic activity of designed amyloid fibrils using a high-pressure stopped-flow system with rapid UV/Vis absorption detection. Complementary FT-IR spectroscopic data revealed a remarkably high pressure and temperature stability of the fibrillar systems. High pressure enhances the esterase activity as a consequence of a negative activation volume at all temperatures (about -14 cm(3)  mol(-1) ). The enhancement is sustained in the whole temperature range covered, which allows a further acceleration of the enzymatic activity at high temperatures (activation energy 45-60 kJ mol(-1) ). Our data reveal the great potential of using both pressure and temperature modulation to optimize the enzyme efficiency of catalytic amyloid fibrils.
authors: Fakultät für Chemie und Chemische Biologie, TU Dortmund, Otto-Hahn-Strasse 4a, 44227, Dortmund, Germany.
authors: Trung Quan
authors: TQ
authors: Luong
authors: Fakultät für Chemie und Chemische Biologie, TU Dortmund, Otto-Hahn-Strasse 4a, 44227, Dortmund, Germany.
authors: Nelli
authors: N
authors: Erwin
authors: Institut für Stochastik, Universität Ulm, Germany.
authors: Matthias
authors: M
authors: Neumann
authors: Institut für Pharmazeutische Biotechnologie, Universität Ulm, Helmholtzstrasse 8/1, 89081, Ulm, Germany.
authors: Andreas
authors: A
authors: Schmidt
authors: Institut für Pharmazeutische Biotechnologie, Universität Ulm, Helmholtzstrasse 8/1, 89081, Ulm, Germany.
authors: Cornelia
authors: C
authors: Loos
authors: Institut für Stochastik, Universität Ulm, Germany.
authors: Volker
authors: V
authors: Schmidt
authors: Institut für Pharmazeutische Biotechnologie, Universität Ulm, Helmholtzstrasse 8/1, 89081, Ulm, Germany. marcus.faendrich@uni-ulm.de.
authors: Marcus
authors: M
authors: Fändrich
authors: Fakultät für Chemie und Chemische Biologie, TU Dortmund, Otto-Hahn-Strasse 4a, 44227, Dortmund, Germany. roland.winter@tu-dortmund.de.
authors: Roland
authors: R
authors: Winter
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D004950:Esterases / Q000378:metabolism*; D006868:Hydrolysis; D006874:Hydrostatic Pressure; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D056004:Molecular Dynamics Simulation; D011789:Quantum Theory; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D000682:Amyloid; D004950:Esterases
keywords: amyloid fibrils; catalysis; pressure; stopped-flow techniques