title: A Comparative Evaluation of Hydroxycamptothecin Drug Nanorods With and Without Methotrexate Prodrug Functionalization for Drug Delivery.
abstract: We developed a novel self-targeted multi-drug co-delivery system based on rod-shaped 10-hydroxycamptothecin (CPT) nanoanticancer drug (CPT NRs) followed by a surface functionalization with self-targeting PEGylated lipid-conjugated methotrexate (MTX) pro-anticancer drug. The self-targeting effect and in vitro cell viability of the MTX-PEG-CPT NRs on HeLa cells were demonstrated by comparative cellular uptake and MTT assay of the PEG-CPT NRs. In vitro studies showed the feasibility of using this high drug-loading MTX-PEG-CPT NRs in self-targeted drug delivery, controlled-/sustained-release, and synergistic cancer therapy. More importantly, this work would stimulate interest in the use of PEGylated lipid-conjugated MTX by introducing an early-phase tumor-targeting role and then driving a late-phase anticancer role for the highly convergent design of nanomulti-drug, which may advantageously offer a new and simple strategy for simultaneously targeting and treating FA receptor-overexpressing cancer cells.
authors: Department of Physics, Changji University, Changji, 831100, China.
authors: Fuqiang
authors: F
authors: Guo
authors: Department of Physics, Changji University, Changji, 831100, China.
authors: Zhongxiong
authors: Z
authors: Fan
authors: People's Hospital of Xintai City, Xintai, Shandong, 271200, China.
authors: Jinbin
authors: J
authors: Yang
authors: College of Materials, Xiamen University, Xiamen, 361005, China.
authors: Yang
authors: Y
authors: Li
authors: College of Materials, Xiamen University, Xiamen, 361005, China.
authors: Yange
authors: Y
authors: Wang
authors: Department of Physics, Changji University, Changji, 831100, China.
authors: Hai
authors: H
authors: Zhao
authors: The First Affiliated Hospital of Xiamen University, Xiamen, 361003, China. xly885@163.com.
authors: Liya
authors: L
authors: Xie
authors: Department of Physics, Changji University, Changji, 831100, China. houzhenqing@xmu.edu.cn.|College of Materials, Xiamen University, Xiamen, 361005, China. houzhenqing@xmu.edu.cn.
authors: Zhenqing
authors: http://orcid.org/0000-0002-5537-075X
authors: Z
authors: Hou
keywords: 10-Hydroxycamptothecin; Controlled and sustained release; Methotrexate; Self-targeted multi-drug co-delivery system