title: Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue.
abstract: Guanylyl cyclase C (GC-C) is a member of a family of enzymes that metabolize GTP to cGMP and was first identified as a receptor for heat-stable enterotoxin. Guanylin (GNY) has since been identified as an endogenous ligand for GC-C in the intestine of several mammalian species. The GNY/GC-C system regulates ion transportation and pH in the mucosa. Recently, it was reported that GC-C and GNY are involved in lipid metabolism in rat mesenteric adipose tissue macrophages. To examine the role of GC-C and GNY in lipid metabolism in cattle, we used a bovine mesenteric adipocyte primary culture system and a coculture system for bovine adipocytes and GNY-/GC-C-expressing macrophages. Fat droplets were observed to accumulate in bovine mesenteric adipocytes cultured alone, whereas few fat droplets accumulated in adipocytes indirectly cocultured with macrophages. We also observed that GC-C was present in bovine mesenteric adipose tissue, and that fat droplet accumulation decreased afterin vitroGNY administration. Expressions of mRNAs encoding lipogenic factors decreased significantly in adipocytes after either coculture or GNY administration. These results suggest that the GNY/GC-C system is part of the control system for lipid accumulation in bovine mesenteric adipose tissue.
authors: Department of Veterinary Anatomy, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
authors: Masahiro
authors: M
authors: Yasuda
authors: Department of Veterinary Anatomy, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
authors: Jyunya
authors: J
authors: Kawabata
authors: Frontier Science Research Center, University of Miyazaki, Miyazaki 889-1692, Japan.
authors: Sayaka
authors: S
authors: Akieda-Asai
authors: Department of Veterinary Anatomy, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
authors: Tetsuo
authors: T
authors: Nasu
authors: Frontier Science Research Center, University of Miyazaki, Miyazaki 889-1692, Japan.
authors: Yukari
authors: Y
authors: Date
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D018920:Coculture Techniques / Q000662:veterinary; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D006162:Guanylate Cyclase; D050356:Lipid Metabolism / Q000187:drug effects / Q000502:physiology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D045265:Natriuretic Peptides / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D000074261:Receptors, Enterotoxin / Q000378:metabolism*
chemical_list: D005768:Gastrointestinal Hormones; D045265:Natriuretic Peptides; C072685:guanylin; D006162:Guanylate Cyclase; D000074261:Receptors, Enterotoxin
keywords: cattle; guanylin; guanylyl cyclase C; lipogenic factor; mesenteric adipose tissue