title: Triticonazole enantiomers: Separation by supercritical fluid chromatography and the effect of the chromatographic conditions.
abstract: Enantiomeric pairs of triticonazole have been successfully separated by supercritical fluid chromatography coupled with a tris(3,5-dimethylphenylcarbamoyl) cellulose-coated chiral stationary phase in this work. The effects of co-solvent, dissolution solvent, flow rate, backpressure, and column temperature have been studied in detail with respect to retention, selectivity, and resolution of triticonazole. As indicated, the co-solvents mostly affected the retention factors and resolution, due to the different molecular structure and polarity. In addition, the dissolution solvents, namely, chloromethanes and alcohols, have been also important for enantioseparation because of the different interaction with stationary phase. Higher flow rate and backpressure led to faster elution of the triticonazole molecules, and the change of column temperature showed slight effect on the resolution of triticonazole racemate. Moreover, a comparative separation experiment between supercritical fluid chromatography and high performance liquid chromatography revealed that chiral supercritical fluid chromatography gave the 3.5 times value of Rs/tR2than high performance liquid chromatography, which demonstrated that supercritical fluid chromatography had much higher separation efficiency.
authors: School of Chemistry and Environment, South China Normal University, Guangzhou, China.
authors: Jianfeng
authors: J
authors: He
authors: School of Chemistry and Environment, South China Normal University, Guangzhou, China.
authors: Jun
authors: J
authors: Fan
authors: School of Chemistry and Environment, South China Normal University, Guangzhou, China.
authors: Yilun
authors: Y
authors: Yan
authors: Guangdong YanJie Pharmatech Co. Ltd, Guangzhou, China.
authors: Xiaodong
authors: X
authors: Chen
authors: Guangdong YanJie Pharmatech Co. Ltd, Guangzhou, China.
authors: Tai
authors: T
authors: Wang
authors: Yingde Greatchem Chemicals Co. Ltd, Yingde, China.
authors: Yaomou
authors: Y
authors: Zhang
authors: School of Chemistry and Environment, South China Normal University, Guangzhou, China.
authors: Weiguang
authors: W
authors: Zhang
keywords: Chiral separation; Chromatographic conditions; Supercritical fluid chromatography; Triticonazole