title: Insights into Interspecific Hybridization Events in Allotetraploid Cotton Formation from Characterization of a Gene-Regulating Leaf Shape.
abstract: The morphology of cotton leaves varies considerably. Phenotypes, including okra, sea-island, super-okra, and broad leaf, are controlled by a multiple allele locus, L2Okra leaf (L2°) is an incomplete mutation that alters leaf shape by increasing the length of lobes with deeper sinuses. Using a map-based cloning strategy, we cloned the L2locus gene, which encodes a LATE MERISTEM IDENTITY 1 (LMI1)-like transcription factor (GhOKRA). Silencing GhOKRA leads to a change in phenotype from okra to broad leaf. Overexpression of GhOKRA in Arabidopsis thaliana greatly increases the degree of the leaf lobes and changes the leaf shape. Premature termination of translation in GhOKRA results in the production of broad leaves. The sequences of OKRA from diploid progenitor D-genome species, and wild races and domesticated allotetraploid cottons in Gossypium hirsutum show that a premature termination mutation occurred before and after the formation of tetraploid cotton, respectively. This study provides genomic insights into the two interspecific hybridization events: one produced the present broad leaf and another formed okra leaf phenotype with complete OKRA, that occurred during allotetraploid cotton formation.
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 210095, China.
authors: Lijing
authors: L
authors: Chang
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 210095, China.
authors: Lei
authors: L
authors: Fang
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 210095, China.
authors: Yajuan
authors: Y
authors: Zhu
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 210095, China.
authors: Huaitong
authors: H
authors: Wu
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 210095, China.
authors: Zhiyuan
authors: Z
authors: Zhang
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 210095, China.
authors: Chunxiao
authors: C
authors: Liu
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 210095, China.
authors: Xinghe
authors: X
authors: Li
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 210095, China cotton@njau.edu.cn.
authors: Tianzhen
authors: T
authors: Zhang
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000033:anatomy & histology / Q000235:genetics*; D006824:Hybridization, Genetic; D010641:Phenotype; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D057891:Tetraploidy; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: HD-ZIP I transcription factor; cotton; evolution; interspecific hybridization; leaf shape