title: Next-Generation Sequencing: Role in Gynecologic Cancers.
abstract: Next-generation sequencing (NGS) has risen to the forefront of tumor analysis and has enabled unprecedented advances in the molecular profiling of solid tumors. Through massively parallel sequencing, previously unrecognized genomic alterations have been unveiled in many malignancies, including gynecologic cancers, thus expanding the potential repertoire for the use of targeted therapies. NGS has expanded the understanding of the genomic foundation of gynecologic malignancies and has allowed identification of germline and somatic mutations associated with cancer development, enabled tumor reclassification, and helped determine mechanisms of treatment resistance. NGS has also facilitated rationale therapeutic strategies based on actionable molecular aberrations. However, issues remain regarding cost and clinical utility. This review covers NGS analysis of and its impact thus far on gynecologic cancers, specifically ovarian, endometrial, cervical, and vulvar cancers.
authors: From the Departments of Obstetrics and Gynecology, Division of Gynecologic Oncology, Brigham and Women's Hospital; and Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts.
authors: Tarra
authors: T
authors: Evans
authors: From the Departments of Obstetrics and Gynecology, Division of Gynecologic Oncology, Brigham and Women's Hospital; and Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts.
authors: Ursula
authors: U
authors: Matulonis
mesh_terms: D005260:Female; D005833:Genital Neoplasms, Female / Q000175:diagnosis*; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*