title: Preparation of drug-in-cyclodextrin-in-liposomes at a large scale using a membrane contactor: Application to trans-anethole.
abstract: The present study aimed to prepare liposomes loaded with cyclodextrin/drug inclusion complexes at a pilot scale based on the ethanol injection technique. Anethole (ANE), a major component of anise and fennel essential oils, was used as a model of a volatile and highly hydrophobic drug. Membrane contactor (600mL) and a pilot plant (3L) were used for liposome production. The liposome preparations obtained were characterized for size, polydispersity index, zeta potential, morphology, stability and ANE release rate. All experimental set-ups were shown to be appropriate for the preparation of small, multilamellar vesicles with narrow size distribution and good stability at 4°C. The drug release study showed that only a small amount of ANE was released from liposome formulations after 21days of storage at 4°C. The loading rate of ANE was higher when ethanol was evaporated directly on the pilot plant compared to a rotary evaporation.
authors: Bioactive Molecules Research Group, Faculty of Sciences, Doctoral School of Sciences and Technologies, Lebanese University, Lebanon; Laboratoire d'Automatique et de Génie des Procédés, Université Claude Bernard Lyon 1, France. Electronic address: gharib_riham@hotmail.com.
authors: Riham
authors: R
authors: Gharib
authors: Bioactive Molecules Research Group, Faculty of Sciences, Doctoral School of Sciences and Technologies, Lebanese University, Lebanon. Electronic address: hgreige@ul.edu.lb.
authors: Hélène
authors: H
authors: Greige-Gerges
authors: Bioactive Molecules Research Group, Faculty of Sciences, Doctoral School of Sciences and Technologies, Lebanese University, Lebanon. Electronic address: ajraij@ul.edu.lb.
authors: Alia
authors: A
authors: Jraij
authors: Bioactive Molecules Research Group, Faculty of Sciences, Doctoral School of Sciences and Technologies, Lebanese University, Lebanon. Electronic address: lauezova@ul.edu.lb.
authors: Lizette
authors: L
authors: Auezova
authors: Laboratoire d'Automatique et de Génie des Procédés, Université Claude Bernard Lyon 1, France. Electronic address: charcosset@lagep.univ-lyon1.fr.
authors: Catherine
authors: C
authors: Charcosset
mesh_terms: D000081248:Allylbenzene Derivatives; D000840:Anisoles / Q000737:chemistry / Q000493:pharmacokinetics*; D055731:Carbohydrate Biochemistry / Q000295:instrumentation / Q000379:methods; D003505:Cyclodextrins / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D065546:Drug Liberation; D004355:Drug Stability; D000067493:Dynamic Light Scattering; D004867:Equipment Design; D008081:Liposomes / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission
chemical_list: D000081248:Allylbenzene Derivatives; D000840:Anisoles; D003505:Cyclodextrins; D004337:Drug Carriers; D008081:Liposomes; C006578:anethole
keywords: Anethole; Cyclodextrin; Double loading; Liposome; Membrane contactor; Scale-up