title: Effect of pretreatment and enzymatic hydrolysis on the physical-chemical composition and morphologic structure of sugarcane bagasse and sugarcane straw.
abstract: The present work aimed to study the effect of the pretreatment of sugarcane bagasse and straw with microwave irradiation in aqueous and acid glycerol solutions on their chemical composition, fiber structure and the efficiency of subsequent enzymatic hydrolysis. Thermogravimetric analysis showed that the pretreatment acted mainly on the lignin and hemicellulose fractions of the bagasse, whereas, in the straw, lesser structural and chemical changes were observed. The images from transmission electron microscopy (TEM) revealed that treating bagasse and straw with acid glycerol solution loosened the cell walls and there was a breakdown in the pit membrane. The treated material was submitted to hydrolysis for 72h and higher yields of reducing sugars were observed compared to the untreated material (250.9mg/g from straw and 197.4mg/g from bagasse). TEM images after hydrolysis confirmed the possible points of access of the enzymes to the secondary cell wall region of the pretreated biomass.
authors: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil. Electronic address: marciamoretty@yahoo.com.br.
authors: Marcia Maria de Souza
authors: MMS
authors: Moretti
authors: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors: Olavo Micali
authors: OM
authors: Perrone
authors: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors: Christiane da Costa Carreira
authors: CDCC
authors: Nunes
authors: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors: Sebastião
authors: S
authors: Taboga
authors: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors: Maurício
authors: M
authors: Boscolo
authors: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors: Roberto
authors: R
authors: da Silva
authors: Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
authors: Eleni
authors: E
authors: Gomes
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002473:Cell Wall / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008872:Microwaves; D009005:Monosaccharides / Q000737:chemistry / Q000378:metabolism; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D012996:Solutions / Q000737:chemistry; D013818:Thermogravimetry
chemical_list: D004798:Enzymes; D009005:Monosaccharides; D011134:Polysaccharides; D012996:Solutions; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D005990:Glycerol
keywords: Glycerol; Microwave; Pretreatment; Saccharification; Sugarcane biomass; Sulfuric acid