title: Capillary electrophoresis chemiluminescence assay of naphthol isomers in human urine and river water using Ni(IV) complex-luminol system.
abstract: A capillary electrophoresis method involving online indirect chemiluminescence (CL) detection was used to determine naphthol (NAP) isomers. The method was based on the quenching effect of 1- and 2-NAP on a new CL reaction of luminol with Ni(IV) complex in an alkaline medium. Separation was conducted with a 25.0 mM sodium borate buffer containing 0.8 mmol/L luminol. Under optimized conditions, 1- and 2-NAP were baseline separated and detected in less than 8 min. The limits of detection of 1- and 2-NAP were 3.1 and 2.7 μg/L, respectively (S/N = 3), with a linear range of 4.0-80.0 μg/L (r > 0.995). Analysis of real samples demonstrated that the spiked recoveries were in the range of 89.2-107.5% (n = 3). The proposed method was successfully used to determine 1- and 2-NAP contents in three environmental water samples and 14 human urine samples. No derivatization or tedious pretreatment was required in the analysis. The proposed method is a potential approach for routine tests of naphthol isomers in a facile CE-CL system.
authors: Key Laboratory of Forensic Medicine of Hebei Province, Shijiazhuang, Hebei Province, P. R. China.|School of Public Health, Hebei Medical University, Shijiazhuang, Hebei Province, P. R. China.
authors: Xiangdong
authors: X
authors: Xu
authors: School of Public Health, Hebei Medical University, Shijiazhuang, Hebei Province, P. R. China.
authors: Nan
authors: N
authors: Li
authors: School of Public Health, Hebei Medical University, Shijiazhuang, Hebei Province, P. R. China.
authors: Xiang
authors: X
authors: Li
authors: School of Public Health, Hebei Medical University, Shijiazhuang, Hebei Province, P. R. China.
authors: Hongmei
authors: H
authors: Shi
authors: Key Laboratory of Forensic Medicine of Hebei Province, Shijiazhuang, Hebei Province, P. R. China.
authors: Chunling
authors: C
authors: Ma
authors: School of Public Health, Hebei Medical University, Shijiazhuang, Hebei Province, P. R. China.
authors: Weijun
authors: W
authors: Kang
authors: Key Laboratory of Forensic Medicine of Hebei Province, Shijiazhuang, Hebei Province, P. R. China.
authors: Bin
authors: B
authors: Cong
mesh_terms: D000328:Adult; D000368:Aged; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D008875:Middle Aged; D009284:Naphthols / Q000032:analysis / Q000737:chemistry / Q000652:urine*; D009532:Nickel / Q000737:chemistry; D015203:Reproducibility of Results; D045483:Rivers / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry; D055815:Young Adult
chemical_list: D009284:Naphthols; D014874:Water Pollutants, Chemical; D008165:Luminol; D009532:Nickel
keywords: Capillary electrophoresis; Chemiluminescence; Naphthol isomers; Ni(IV) complex