title: A serum 6-miRNA panel as a novel non-invasive biomarker for meningioma.
abstract: Circulating microRNAs (miRNAs) hold great promise as novel clinically blood-based biomarkers for cancer diagnosis and prognosis. However, little is known about their impact in meningioma. The TLDA assay was performed as an initial survey to determine the serum miRNA expression profile in two pooled samples from 20 pre-operative meningiomas and 20 matched healthy controls. Selected candidate miRNAs were subsequently validated individually in another 210 patients and 210 healthy controls from two independent cohorts by qRT-PCR. The serum levels of miR-106a-5p, miR-219-5p, miR-375, and miR-409-3p were significantly increased, whereas the serum levels of miR-197 and miR-224 were markedly decreased. The area under the ROC curve (AUC) for the six combined miRNAs was 0.778. The 4 increased miRNAs were significantly decreased, while the 2 decreased miRNAs were significantly increased after tumor removal. Furthermore, the expression levels of miR-224 were associated with sex, and the expression levels of miR-219-5p were positively associated with the clinical stages of meningioma. Finally, the high expression of miR-409-3p and low expression of miR-224 were significantly correlated with higher recurrence rates. The present study revealed that the panel of 6 serum miRNA may have the potential to be used clinically as an auxiliary tool for meningioma patients.
authors: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
authors: Feng
authors: F
authors: Zhi
authors: Department of Neurosurgery, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
authors: Naiyuan
authors: N
authors: Shao
authors: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
authors: Bowen
authors: B
authors: Li
authors: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
authors: Lian
authors: L
authors: Xue
authors: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
authors: Danni
authors: D
authors: Deng
authors: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
authors: Yuan
authors: Y
authors: Xu
authors: Department of Neurosurgery, Second Affiliated Hospital of Soochow University, Soochow, Jiangsu, China.
authors: Qing
authors: Q
authors: Lan
authors: Department of Neurosurgery, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
authors: Ya
authors: Y
authors: Peng
authors: Modern Medical Research Center, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.|Department of Neurosurgery, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
authors: Yilin
authors: Y
authors: Yang
mesh_terms: D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005819:Genetic Markers; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D008579:Meningioma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D005819:Genetic Markers; C546140:MIRN224 microRNA, human; C572367:MIRN409 microRNA, human; D035683:MicroRNAs