title: [BRAF V600E mutation in thyroid nodules in Argentina].
abstract: This prospective study analyzed the frequency of V600E mutation of oncogene BRAF in patients operated for benign thyroid nodules and for papillary thyroid cancer in an Argentine population. In patients with papillary thyroid cancer we compared clinicopathological characteristics between those harboring BRAF mutation and those without it. Twenty five consecutive patients operated for benign nodules and for papillary carcinoma were prospectively included. Fresh tissue samples of thyroid nodules and of adjacent thyroid parenchyma were obtained. DNA was extracted and amplified by amplification refractory mutation system polymerase chain reaction (ARMS PCR). Direct sequencing was performed in four samples. Of those patients operated for papillary thyroid cancer, 77% harbored BRAF mutation. All samples from adjacent thyroid parenchyma and from patients operated for benign nodules tested negative for the mutation. Direct sequencing confirmed the results obtained by ARMS PCR. Patients with BRAF mutation were significantly older at the time of diagnosis (BRAF+ 47.7 ± 12.7 years vs. BRAF- 24.7 ± 8.1 years, p < 0.01). Nine out of ten papillary carcinomas with BRAF mutation corresponded to the classic histological subtype, which was not observed in BRAF negative tumors (p < 0.02). In conclusion, we found a high frequency of BRAF V600E mutation in this population of patients operated for papillary thyroid carcinoma in Argentina. These results are consistent with those reported in the literature.
authors: División Endocrinología, Hospital General de Agudos José María Ramos Mejía, Buenos Aires, Argentina. E-mail: veronicailera@gmail.com.
authors: Verónica
authors: V
authors: Ilera
authors: Sección Biología Molecular, División Laboratorio Central, Hospital General de Agudos José María Ramos Mejía, Buenos Aires, Argentina.
authors: Ricardo
authors: R
authors: Dourisboure
authors: División Anatomía Patológica, Hospital General de Agudos José María Ramos Mejía, Buenos Aires, Argentina.
authors: Antonio
authors: A
authors: Colobraro
authors: División Endocrinología, Hospital General de Agudos José María Ramos Mejía, Buenos Aires, Argentina.
authors: María Del Carmen
authors: Mdel C
authors: Silva Croome
authors: División Cirugía. Hospital General de Agudos José María Ramos Mejía, Buenos Aires, Argentina.
authors: Gustavo
authors: G
authors: Olstein
authors: División Endocrinología, Hospital General de Agudos José María Ramos Mejía, Buenos Aires, Argentina.
authors: Alicia
authors: A
authors: Gauna
mesh_terms: D000328:Adult; D000368:Aged; D001118:Argentina; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002291:Carcinoma, Papillary; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011446:Prospective Studies; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D016606:Thyroid Nodule / Q000235:genetics* / Q000473:pathology
chemical_list: D048493:Proto-Oncogene Proteins B-raf
keywords: BRAF; molecular testing; papillary thyroid cancer; thyroid nodules