title: Activation of 5-HT2A/2C receptors reduces the excitability of cultured cortical neurons.
abstract: The abundant forebrain serotonergic projections are believed to modulate the activities of cortical neurons. 5-HT2 receptor among multiple subtypes of serotonin receptors contributes to the modulation of excitability, synaptic transmissions and plasticity. In the present study, whole-cell patch-clamp recording was adopted to examine whether activation of 5-HT2A/2C receptors would have any impact on the excitability of cultured cortical neurons. We found that 2,5-Dimethoxy-4-iodoamphetamine (DOI), a selective 5-HT2A/2C receptor agonist, rapidly and reversibly depressed spontaneous action potentials mimicking the effect of serotonin. The decreased excitability was also observed for current-evoked firing. Additionally DOI increased neuronal input resistance. Hyperpolarization-activated cyclic nucleotide-gated cationic channels (HCN) did not account for the inhibition of spontaneous firing. The synaptic contribution was ruled out in that DOI augmented excitation and attenuated inhibition to actually favor an increase in the excitability. Our findings revealed that activation of 5-HT2A/2C receptors reduces neuronal excitability, which would deepen our understanding of serotonergic modulation of cortical activities.
authors: Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China; Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou, Guangdong, 510530, China.
authors: Lingli
authors: L
authors: Hu
authors: Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China; Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou, Guangdong, 510530, China.
authors: Chunhua
authors: C
authors: Liu
authors: Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China; Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou, Guangdong, 510530, China.
authors: Minyan
authors: M
authors: Dang
authors: Department of Otolaryngology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei, 230001, China.
authors: Bin
authors: B
authors: Luo
authors: Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China; Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou, Guangdong, 510530, China. Electronic address: guo_yiping@gibh.ac.cn.
authors: Yiping
authors: Y
authors: Guo
authors: Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China; Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou, Guangdong, 510530, China; Department of Neurobiology and Biophysics, University of Science and Technology of China, Hefei, 230027, China. Electronic address: wang_haitao@gibh.ac.cn.
authors: Haitao
authors: H
authors: Wang
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000662:Amphetamines / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects*; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000037:antagonists & inhibitors; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D011743:Pyrimidines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000494:pharmacology*; D058826:Serotonin 5-HT2 Receptor Agonists / Q000494:pharmacology*; D009435:Synaptic Transmission / Q000187:drug effects
chemical_list: D000662:Amphetamines; D054815:Cyclic Nucleotide-Gated Cation Channels; D011743:Pyrimidines; D058826:Serotonin 5-HT2 Receptor Agonists; C082246:ICI D2788; D012701:Serotonin; C015952:4-iodo-2,5-dimethoxyphenylisopropylamine
keywords: 5-HT(2A/2C) receptors; Excitability; Patch-clamp; Synaptic transmission