title: Establishment of induced pluripotent stem cell (iPSC) line from a 75-year old patient with late onset Alzheimer's disease (LOAD).
abstract: Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterised 75-year old woman with late onset Alzheimer's disease (LOAD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously towards the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to further study the pathomechanism of sporadic AD, to identify early biomarkers and also for drug testing and gene therapy studies.
authors: BioTalentum Ltd., Gödöllő, Hungary; Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllő, Hungary.
authors: Zsuzsanna
authors: Z
authors: Táncos
authors: BioTalentum Ltd., Gödöllő, Hungary.
authors: Eszter
authors: E
authors: Varga
authors: BioTalentum Ltd., Gödöllő, Hungary.
authors: Eszter
authors: E
authors: Kovács
authors: BioTalentum Ltd., Gödöllő, Hungary; Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllő, Hungary.
authors: András
authors: A
authors: Dinnyés
authors: BioTalentum Ltd., Gödöllő, Hungary.
authors: Julianna
authors: J
authors: Kobolák