title: Gene expression profile analysis of dbpA knockdown in colorectal cancer cells.
abstract: DNA-binding protein A (dbpA) has been reported associated with the pathogenesis and development of various cancers. However, no evidence showed the gene expression profiling alternation involved in dbpA knockdown in colorectal cancer (CRC) cells. Small interference RNA (siRNA) method was used to knock down dbpA expression in SW620 cells. The changes of gene expression profiles in dbpA knockdown SW620 cells were determined by microarray analysis and Western blot. A total of 578 genes expressed differentially (twofold change), 181 genes were up-regulated, and 397 down-regulated in the dbpA knockdown group in comparison with the control group. The discrimination reliability was further verified by principal component analysis and Pearson correlation. Gene ontology (GO) pathway analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the most significantly expressed genes were linked to MAPK signaling pathway. Furthermore, Western blot verified that dbpA knockdown directly inhibited the activations of TAK1, p38, and JNK in CRC cells. In conclusion, dbpA knockdown in SW620 cells altered the expression of carcinogenesis-associated genes in CRC and the involvement of dbpA on CRC might through MAPK signaling pathway, which might provide valuable evidence to further investigate the correlation between dbpA expression and CRC development.
authors: Department of General Surgery, Shaanxi Provincial People's Hospital, The Third Affiliated Hospital, Medical College, Xi'an Jiao Tong University, Xi'an, 710068, China.
authors: Ruiting
authors: R
authors: Liu
authors: Department of General Surgery, Shaanxi Provincial People's Hospital, The Third Affiliated Hospital, Medical College, Xi'an Jiao Tong University, Xi'an, 710068, China.
authors: Guorong
authors: G
authors: Wang
authors: Department of Hepatobiliary Surgery, The First Affiliated Hospital, Medical College, Xi'an Jiao Tong University, Xi'an, 710061, China.
authors: Chang
authors: C
authors: Liu
authors: Department of General Surgery, Shaanxi Provincial People's Hospital, The Third Affiliated Hospital, Medical College, Xi'an Jiao Tong University, Xi'an, 710068, China.
authors: Jian
authors: J
authors: Qiu
authors: Department of General Surgery, Shaanxi Provincial People's Hospital, The Third Affiliated Hospital, Medical College, Xi'an Jiao Tong University, Xi'an, 710068, China.
authors: Likun
authors: L
authors: Yan
authors: Department of General Surgery, Shaanxi Provincial People's Hospital, The Third Affiliated Hospital, Medical College, Xi'an Jiao Tong University, Xi'an, 710068, China.
authors: Xiaojun
authors: X
authors: Li
authors: Department of General Surgery, Shaanxi Provincial People's Hospital, The Third Affiliated Hospital, Medical College, Xi'an Jiao Tong University, Xi'an, 710068, China.
authors: Xiaoqiang
authors: X
authors: Wang
mesh_terms: D015153:Blotting, Western; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D020868:Gene Silencing; D005822:Genetic Vectors / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011786:Quality Control; D034741:RNA, Small Interfering / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000235:genetics
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D006360:Heat-Shock Proteins; D034741:RNA, Small Interfering; C094867:YBX3 protein, human
keywords: colorectal cancer; dpbA; gene expression profiling; microassay; small interfering RNA