title: Analysis of fucosylation in liver-secreted N-glycoproteins from human hepatocellular carcinoma plasma using liquid chromatography with tandem mass spectrometry.
abstract: Fucosylation of N-glycoproteins has been implicated in various diseases, such as hepatocellular carcinoma (HCC). However, few studies have performed site-specific analysis of fucosylation in liver-secreted proteins. In this study, we characterized the fucosylation patterns of liver-secreted proteins in HCC plasma using a workflow to identify site-specific N-glycoproteins, where characteristic B- and/or Y-ion series with and without fucose in collision-induced dissociation were used in tandem mass spectrometry. In total, 71 fucosylated N-glycopeptides from 13 major liver-secreted proteins in human plasma were globally identified by LC-MS/MS. Additionally, 37 fucosylated N-glycopeptides were newly identified from nine liver-secreted proteins, including alpha-1-antichymotrypsin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, ceruloplasmin, alpha-1-acid glycoprotein 1/2, alpha-2-macroglobulin, serotransferrin, and beta-2-glycoprotein 1. Of the fucosylated N-glycopeptides, bi- and tri-antennary glycoforms were the most common ones identified in liver-secreted proteins from HCC plasma. Therefore, we suggest that this analytical method is effective for characterizing fucosylation in liver-secreted proteins. Graphical abstract A global map of fucosylated and non-fucosylated glycopeptides from 13 liver-secreted glycoproteins in hepatocellular carcinoma plasma.
authors: Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, Chungbuk, 28119, Republic of Korea.
authors: Eun Sun
authors: ES
authors: Ji
authors: Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, Chungbuk, 28119, Republic of Korea.
authors: Heeyoun
authors: H
authors: Hwang
authors: Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, Chungbuk, 28119, Republic of Korea.|Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors: Gun Wook
authors: GW
authors: Park
authors: Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, Chungbuk, 28119, Republic of Korea.
authors: Ju Yeon
authors: JY
authors: Lee
authors: Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, Chungbuk, 28119, Republic of Korea.|Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors: Hyun Kyoung
authors: HK
authors: Lee
authors: Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, Chungbuk, 28119, Republic of Korea.|Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors: Na Young
authors: NY
authors: Choi
authors: Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, Chungbuk, 28119, Republic of Korea.|Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors: Hoi Keun
authors: HK
authors: Jeong
authors: Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, Chungbuk, 28119, Republic of Korea.|Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors: Kwang Hoe
authors: KH
authors: Kim
authors: Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, Chungbuk, 28119, Republic of Korea.
authors: Jin Young
authors: JY
authors: Kim
authors: Department of Chemistry, Hannam University, Daejeon, 306-791, Republic of Korea.
authors: Seungho
authors: S
authors: Lee
authors: Department of Biomedical Science, Cheongju University, Cheongju, 28503, Republic of Korea. ahnyh@cju.ac.kr.
authors: Yeong Hee
authors: YH
authors: Ahn
authors: Biomedical Omics Group, Korea Basic Science Institute, 162 YeonGuDanji-Ro, Ochang-eup, Cheongju, Chungbuk, 28119, Republic of Korea. jongshin@kbsi.re.kr.|Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, Republic of Korea. jongshin@kbsi.re.kr.
authors: Jong Shin
authors: JS
authors: Yoo
mesh_terms: D002240:Carbohydrate Sequence; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D002853:Chromatography, Liquid; D005643:Fucose / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D058977:Molecular Sequence Annotation; D009363:Neoplasm Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D053719:Tandem Mass Spectrometry
chemical_list: D006023:Glycoproteins; D009363:Neoplasm Proteins; D005643:Fucose
keywords: Fucosylation; Human plasma; Liquid chromatography; Liver-secreted glycoproteins; Mass spectrometry; N-Glycoproteins