title: Synthesis and characterization of non-toxic and thermo-sensitive poly(N-isopropylacrylamide)-grafted cashew gum nanoparticles as a potential epirubicin delivery matrix.
abstract: Cashew gum (CG) was grafted with N-isopropylacrylamide (NIPA) by radical polymerization to originate a stimuli-sensitive copolymer for drug delivery purposes. NMR and IR spectroscopy confirmed the insertion of NIPA onto the cashew gum chains. The graft copolymer (CG:NIPA) demonstrated thermal responsiveness. The critical aggregation concentration of the copolymers at 25°C was higher than at 50°C. At temperatures lower than the LCST, the nanoparticle size ranged from 12 to 21nm, depending on the CG:NIPA ratio, but above the LCST the particles aggregated, increasing the particle size. Regarding the potential for future oral application, the nanoparticles showed no cytotoxic activity against the Caco-2 and HT29-MTX intestine cell lines. Epirubicin was encapsulated into nanoparticles of CG-NIPA (1:1), resulting in a 64% association efficiency and 22% loading capacity. Thus, the CG:NIPA graft copolymer demonstrates good potential for used in controlled drug delivery systems.
authors: Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Caixa Postal 6021, Rua Humberto Monte s/n, Campus do Pici, CEP 60455-760 Fortaleza, Brazil.
authors: Clara M W S
authors: CM
authors: Abreu
authors: Departamento de Química Analítica e Físico-Química, Universidade Federal do Ceará, Caixa Postal 6021, Rua Humberto Monte s/n, Campus do Pici, CEP 60455-760 Fortaleza, Brazil.
authors: Haroldo C B
authors: HC
authors: Paula
authors: CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde and Instituto Universitário de Ciências da Saúde, Rua Central de Gandra 1317, 4585-116 Gandra, Portugal.
authors: Vitor
authors: V
authors: Seabra
authors: Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Caixa Postal 6021, Rua Humberto Monte s/n, Campus do Pici, CEP 60455-760 Fortaleza, Brazil.
authors: Judith P A
authors: JP
authors: Feitosa
authors: CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde and Instituto Universitário de Ciências da Saúde, Rua Central de Gandra 1317, 4585-116 Gandra, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal; i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal.
authors: Bruno
authors: B
authors: Sarmento
authors: Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Caixa Postal 6021, Rua Humberto Monte s/n, Campus do Pici, CEP 60455-760 Fortaleza, Brazil. Electronic address: rpaula@dqoi.ufc.br.
authors: Regina C M
authors: RC
authors: de Paula
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D031021:Anacardium / Q000737:chemistry; D018938:Caco-2 Cells; D016503:Drug Delivery Systems / Q000379:methods*; D000067493:Dynamic Light Scattering; D015251:Epirubicin / Q000008:administration & dosage*; D019073:HT29 Cells; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D053149:Plant Gums / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
chemical_list: D000180:Acrylic Resins; D053149:Plant Gums; C052970:poly-N-isopropylacrylamide; D015251:Epirubicin
keywords: Cytotoxicity; Epirubicin; N-isopropylacrylamide; Nanoparticles; Thermo-sensitive