title: A conserved role of the RSC chromatin remodeler in the establishment of nucleosome-depleted regions.
abstract: The occupancy of nucleosomes governs access to the eukaryotic genomes and results from a combination of biophysical features and the effect of ATP-dependent remodelling complexes. Most promoter regions show a conserved pattern characterized by a nucleosome-depleted region (NDR) flanked by nucleosomal arrays. The conserved RSC remodeler was reported to be critical to establish NDR in vivo in budding yeast but other evidences suggested that this activity may not be conserved in fission yeast. By reanalysing and expanding previously published data, we propose that NDR formation requires, at least partially, RSC in both yeast species. We also discuss the most prominent biological role of RSC and the possibility that non-essential subunits do not define alternate versions of the complex.
authors: URPHYM-GEMO, Namur Research College (NARC), The University of Namur, 5000, Namur, Belgium.
authors: Carlo
authors: C
authors: Yague-Sanz
authors: Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC)/Universidad de Salamanca, Campus Miguel de Unamuno, 37007, Salamanca, Spain.
authors: Enrique
authors: E
authors: Vázquez
authors: Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC)/Universidad de Salamanca, Campus Miguel de Unamuno, 37007, Salamanca, Spain.
authors: Mar
authors: M
authors: Sánchez
authors: Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC)/Universidad de Salamanca, Campus Miguel de Unamuno, 37007, Salamanca, Spain.
authors: Francisco
authors: F
authors: Antequera
authors: URPHYM-GEMO, Namur Research College (NARC), The University of Namur, 5000, Namur, Belgium. Damien.Hermand@unamur.be.
authors: Damien
authors: http://orcid.org/0000-0002-1029-5848
authors: D
authors: Hermand
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D009707:Nucleosomes / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D004718:Saccharomycetales / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity
chemical_list: D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D000251:Adenosine Triphosphatases
keywords: Chromatin; Mitosis; Nucleosome; RSC; Yeast