title: Ehrlichia chaffeensis TRP32 is a Nucleomodulin that Directly Regulates Expression of Host Genes Governing Differentiation and Proliferation.
abstract: Ehrlichia chaffeensis is an obligately intracellular bacterium that reprograms the mononuclear phagocyte through diverse effector-host interactions to modulate numerous host cell processes, including transcription. In a previous study, we reported that E. chaffeensis TRP32, a type 1 secreted effector, interacts with multiple host nucleus-associated proteins and also auto-activates reporter gene expression in yeast. In this study, we demonstrate that TRP32 is a nucleomodulin that binds host DNA and alters host gene transcription. TRP32 enters the host cell nucleus via a noncanonical translocation mechanism that involves phosphorylation of Y179 located in a C-terminal tri-tyrosine motif. Both genistein and mutation of Y179 inhibited TRP32 nuclear entry. An electromobility shift assay (EMSA) demonstrated TRP32 host DNA binding via its tandem repeat domain. TRP32 DNA binding and motif preference were further confirmed by supershift assays, as well as competition and mutant probe analyses. Using ChIP-Seq, we determined that TRP32 binds a G-rich motif primarily within ±500 bp of the gene transcription start site. An ontology analysis identified genes involved in processes such as immune cell differentiation, chromatin remodeling, and RNA transcription and processing, as primary TRP32 targets. TRP32 bound genes (n=1223) were distributed on all chromosomes and included several global regulators of proliferation and inflammation such as FOS and JUN, AKT3 and NRAS, and non-coding RNA genes, miRNA 21 and miRNA 142. TRP32 target genes were differentially regulated during infection, the majority of which were repressed, and direct repression/activation of these genes by TRP32 was confirmed in vitro with a cellular luciferase reporter assay.
authors: Departments of Microbiology and Immunology and Pathology.
authors: Tierra R
authors: 0000-0001-9022-1584
authors: TR
authors: Farris
authors: Departments of Microbiology and Immunology and Pathology.
authors: Paige S
authors: PS
authors: Dunphy
authors: Departments of Microbiology and Immunology and Pathology.
authors: Bing
authors: B
authors: Zhu
authors: Departments of Microbiology and Immunology and Pathology.
authors: Clayton E
authors: CE
authors: Kibler
authors: Departments of Microbiology and Immunology and Pathology Departments of Microbiology and Immunology and Pathology Center for Biodefense and Emerging Infectious Diseases Sealy Center for Vaccine Development Institute for Human Infections and Immunity , University of Texas Medical Branch, Galveston, TX jemcbrid@utmb.edu.
authors: Jere W
authors: JW
authors: McBride