title: Spectroscopic and calorimetric studies on the interaction between PAMAM G4-OH and 5-fluorouracil in aqueous solutions.
abstract: The results of spectroscopic measurements (an increase in solubility, equilibrium dialysis,1H NMR titration) and calorimetric measurements (isothermal titration ITC) indicate spontaneous (ΔG<0) binding of 5-fluorouracil molecules by PAMAM G4-OH dendrimer with terminal hydroxyl groups in an aqueous solution. PAMAM G4-OH dendrimer bonds about n=8±1 molecules of the drug with an equilibrium constant of K=70±10. The process of saturating the dendrimer active sites by the drug molecules is exothermal (ΔH<0) and is accompanied by an advantageous change in entropy (ΔS>0). The parameters of binding 5-fluorouracil by PAMAM G4-OH dendrimer were compared with those of binding this drug by the macromolecules of PAMAM G3-OH and G5-OH.
authors: Department of Physical Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 165, Lodz 90-236, Poland. Electronic address: adam.buczkowski@chemia.uni.lodz.pl.
authors: Adam
authors: A
authors: Buczkowski
authors: Department of Inorganic and Analytical Chemistry, Faculty of Chemistry, University of Lodz, Tamka 12, 91-403 Lodz, Poland.
authors: Pawel
authors: P
authors: Urbaniak
authors: Department of Physical Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 165, Lodz 90-236, Poland.
authors: Henryk
authors: H
authors: Piekarski
authors: Department of Physical Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 165, Lodz 90-236, Poland. Electronic address: paleczb@uni.lodz.pl.
authors: Bartlomiej
authors: B
authors: Palecz
mesh_terms: D002151:Calorimetry / Q000379:methods*; D050091:Dendrimers / Q000737:chemistry*; D005472:Fluorouracil / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D012996:Solutions; D013696:Temperature
chemical_list: D050091:Dendrimers; D009757:Nylons; C520550:PAMAM-G4; D012996:Solutions; D005472:Fluorouracil
keywords: (1)H NMR spectroscopy; 5-Fluorouracil; Equilibrium dialysis; Isothermal titration calorimetry; PAMAM G4-OH; Solubility measurements