title: Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique.
abstract: To investigate the protein profiling of buffalo oocytes at the germinal vesicle (GV) stage and metaphase II (MII) stage, an iTRAQ-based strategy was applied. A total of 3,763 proteins were identified, which representing the largest buffalo oocytes proteome dataset to date. Among these proteins identified, 173 proteins were differentially expressed in GV oocytes and competent MII oocytes, and 146 proteins were differentially abundant in competent and incompetent matured oocytes. Functional and KEGG pathway analysis revealed that the up-regulated proteins in competent MII oocytes were related to chromosome segregation, microtubule-based process, protein transport, oxidation reduction, ribosome, and oxidative phosphorylation, etc., in comparison with GV and incompetent MII oocytes. This is the first proteomic report on buffalo oocytes from different maturation stages and developmental competent status. These data will provide valuable information for understanding the molecular mechanism underlying buffalo oocyte maturation, and these proteins may potentially act as markers to predict developmental competence of buffalo oocyte during in vitro maturation.
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530005, China.|State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Engineering Research Center for Protein Drugs, National Center for Protein Sciences, Beijing Institute of Radiation Medicine, Beijing 102206, China.
authors: Lingsheng
authors: L
authors: Chen
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Engineering Research Center for Protein Drugs, National Center for Protein Sciences, Beijing Institute of Radiation Medicine, Beijing 102206, China.|Chemical Proteomics Center &State Key Laboratory of Drug Research, Shanghai Institute of Materia Medical, Chinese Academy of Sciences, Shanghai, 201203, China.
authors: Linhui
authors: L
authors: Zhai
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530005, China.|Department of reproductive medicine, Hechi People's hospital of Guangxi, Hechi 547000, China.
authors: Chunfeng
authors: C
authors: Qu
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Engineering Research Center for Protein Drugs, National Center for Protein Sciences, Beijing Institute of Radiation Medicine, Beijing 102206, China.
authors: Chengpu
authors: C
authors: Zhang
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530005, China.
authors: Sheng
authors: S
authors: Li
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Engineering Research Center for Protein Drugs, National Center for Protein Sciences, Beijing Institute of Radiation Medicine, Beijing 102206, China.
authors: Feilin
authors: F
authors: Wu
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Engineering Research Center for Protein Drugs, National Center for Protein Sciences, Beijing Institute of Radiation Medicine, Beijing 102206, China.
authors: Yingzi
authors: Y
authors: Qi
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530005, China.
authors: Fenghua
authors: F
authors: Lu
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Engineering Research Center for Protein Drugs, National Center for Protein Sciences, Beijing Institute of Radiation Medicine, Beijing 102206, China.|Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China.
authors: Ping
authors: P
authors: Xu
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530005, China.
authors: Xiangping
authors: X
authors: Li
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530005, China.
authors: Deshun
authors: D
authors: Shi
mesh_terms: D000818:Animals; D002020:Buffaloes; D002384:Catalysis; D002417:Cattle; D018929:Cell Culture Techniques; D019295:Computational Biology; D054885:Cumulus Cells / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D013058:Mass Spectrometry; D008677:Metaphase; D008870:Microtubules / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis; D010085:Oxidative Phosphorylation; D010455:Peptides; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012270:Ribosomes / Q000378:metabolism; D014357:Trypsin / Q000737:chemistry
chemical_list: D010455:Peptides; D020543:Proteome; D014357:Trypsin