title: Immunogenicity of a recombinant parapoxvirus expressing the spike protein of Porcine epidemic diarrhea virus.
abstract: The parapoxvirus Orf virus (ORFV), has long been recognized for its immunomodulatory properties in permissive and non-permissive animal species. Here, a new recombinant ORFV expressing the full-length spike (S) protein of Porcine epidemic diarrhea virus (PEDV) was generated and its immunogenicity and protective efficacy were evaluated in pigs. The PEDV S was inserted into the ORFV121 gene locus, an immunomodulatory gene that inhibits activation of the NF-κB signalling pathway and contributes to ORFV virulence in the natural host. The recombinant ORFV-PEDV-S virus efficiently and stably expressed the PEDV S protein in cell culture in vitro. Three intramuscular (IM) immunizations with the recombinant ORFV-PEDV-S in 3-week-old pigs elicited robust serum IgG, IgA and neutralizing antibody responses against PEDV. Additionally, IM immunization with the recombinant ORFV-PEDV-S virus protected pigs from clinical signs of porcine epidemic diarrhoea (PED) and reduced virus shedding in faeces upon challenge infection. These results demonstrate the suitability of ORFV121 gene locus as an insertion site for heterologous gene expression and delivery by ORFV-based viral vectors. Additionally, the results provide evidence of the potential of ORFV as a vaccine delivery vector for enteric viral diseases of swine. This study may have important implications for future development of ORFV-vectored vaccines for swine.
authors: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
authors: Kyle S
authors: KS
authors: Hain
authors: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
authors: Lok R
authors: LR
authors: Joshi
authors: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.|National Research Center, Giza, Egypt.
authors: Faten
authors: F
authors: Okda
authors: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
authors: Julie
authors: J
authors: Nelson
authors: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
authors: Aaron
authors: A
authors: Singrey
authors: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
authors: Steven
authors: S
authors: Lawson
authors: Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors: Mathias
authors: M
authors: Martins
authors: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
authors: Angela
authors: A
authors: Pillatzki
authors: Department of Pathobiology, University of Connecticut, Storrs, CT, USA.
authors: Gerald F
authors: GF
authors: Kutish
authors: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
authors: Eric A
authors: EA
authors: Nelson
authors: Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors: Eduardo F
authors: EF
authors: Flores
authors: Animal Disease Research and Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
authors: Diego G
authors: DG
authors: Diel
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D018352:Coronavirus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D007114:Immunization; D018157:Parapoxvirus / Q000235:genetics / Q000378:metabolism; D053485:Porcine epidemic diarrhea virus / Q000235:genetics / Q000276:immunology*; D064370:Spike Glycoprotein, Coronavirus / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D064370:Spike Glycoprotein, Coronavirus; D014765:Viral Vaccines