title: Distribution of fibroblast growth factors and their roles in skin fibroblast cell migration.
abstract: Fibroblast growth factor (FGF)2/basic FGF is a member of the fibroblast growth factor family. Its function in skin wound healing has been well-characterized. However, the function of other FGFs in skin tissues remains to be elucidated. In the present study, FGF expression patterns in heart, liver, skin and kidney tissues were analyzed. Notably, in contrast to other tissues, only four FGFs, FGF2, 7, 10 and 21, were dominant in the skin. To examine FGF function in the wound healing process, mouse NIH3T3 fibroblast cells were treated with FGF2, FGF10 and FGF21, and cell migration was monitored. The results revealed that FGF treatment promoted cell migration, which is an important step in wound healing. In addition, FGF treatment enhanced the activity of c-Jun N-terminal kinase (JNK), a key regulator of fibroblast cell migration. To analyze its role in cell migration, FGF7 was overexpressed in fibroblast cells via a lentivirus system; however, this did not change cell migration speed. FGF2, 7, 10 and 21 were highly expressed in skin tissue, and all except FGF7 regulated fibroblast cell migration and activated JNK. The results of the present study increase our understanding of the role of FGFs in skin wound healing.
authors: Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Yong Huan
authors: YH
authors: Song
authors: The Key Zhejiang Province Laboratory of Biomedicine, School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors: Yu Ting
authors: YT
authors: Zhu
authors: Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Jian
authors: J
authors: Ding
authors: Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Fei Ya
authors: FY
authors: Zhou
authors: Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Ji Xin
authors: JX
authors: Xue
authors: Molecular Evolution Team, Department of Biotechnology R&D, Amicogen, Inc., Jinju, South Gyeongsang 660701, Republic of Korea.
authors: Jin Hee
authors: JH
authors: Jung
authors: Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Zhi Jie
authors: ZJ
authors: Li
authors: Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
authors: Wei Yang
authors: WY
authors: Gao
mesh_terms: D000818:Animals; D002465:Cell Movement; D005346:Fibroblast Growth Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D015870:Gene Expression; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D010802:Phylogeny; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D014945:Wound Healing
chemical_list: D005346:Fibroblast Growth Factors