title: miRNA miR-17-92 cluster is differentially regulated in the imiqumod-treated skin but is not required for imiqumod-induced psoriasis-like dermatitis in mice.
abstract: MicroRNAs (miRNAs) play very important roles in the control of immune cell and keratinocyte development and function and are implicated in skin inflammatory diseases, including psoriasis. miRNA miR-17-92 was reported to promote the differentiation of Th1 and Th1 cells and to regulate cell proliferation and apoptosis. Here we showed that imiquimod (IMQ) differentially regulates the expression of miR-17-92 cluster in the mouse skin, upregulating miR-17 and miR-19 families and downregulating miR-92. To investigate whether miR-17-92 cluster is functionally involved in the psoriasis, we have generated three mutant mice with specific deletion or overexpression of miR-17-92 cluster in keratinocytes, or with deletion of miR-17-92 cluster in T cells. Interestingly, deletion or overexpression of miR-17-92 cluster in keratinocytes, or deletion of miR-17-92 in T cells did not significantly affect IMQ-induced psoriasis-like dermatitis development in the mutant mice compared with wild-type littermates. Thus, miRNA miR-17-92 cluster may not be a key factor regulating imiqumod-induced psoriasis-like dermatitis.
authors: Department of Dermatology, Guangdong provincial Hospital of Chinese Medicine, Guanghzou, China.|Department of Dermatology, Henry Ford Health System, Detroit, MI, USA.|Henry Ford Immunology Program, Henry Ford Health System, Detroit, MI, USA.
authors: Dinghong
authors: D
authors: Wu
authors: Department of Dermatology, Henry Ford Health System, Detroit, MI, USA.|Henry Ford Immunology Program, Henry Ford Health System, Detroit, MI, USA.
authors: Xinling
authors: X
authors: Bi
authors: Department of Dermatology, Henry Ford Health System, Detroit, MI, USA.|Henry Ford Immunology Program, Henry Ford Health System, Detroit, MI, USA.
authors: Le
authors: L
authors: Qu
authors: Department of Dermatology, Guangdong provincial Hospital of Chinese Medicine, Guanghzou, China.|Department of Dermatology, Henry Ford Health System, Detroit, MI, USA.|Henry Ford Immunology Program, Henry Ford Health System, Detroit, MI, USA.
authors: Ling
authors: L
authors: Han
authors: Department of Dermatology, Henry Ford Health System, Detroit, MI, USA.|Henry Ford Immunology Program, Henry Ford Health System, Detroit, MI, USA.
authors: Congcong
authors: C
authors: Yin
authors: Department of Dermatology, Guangdong provincial Hospital of Chinese Medicine, Guanghzou, China.|Department of Dermatology, Henry Ford Health System, Detroit, MI, USA.|Henry Ford Immunology Program, Henry Ford Health System, Detroit, MI, USA.
authors: Jingwen
authors: J
authors: Deng
authors: Department of Cellular Biology and Anatomy, Augusta University, Augusta, GA, USA.
authors: Zheng
authors: Z
authors: Dong
authors: Department of Dermatology, Henry Ford Health System, Detroit, MI, USA.|Henry Ford Immunology Program, Henry Ford Health System, Detroit, MI, USA.|Department of Immunology and Microbiology, Wayne State University, Detroit, MI, USA.|Department of Internal Medicine, Henry Ford Health System, Detroit, MI, USA.
authors: Qing-Sheng
authors: QS
authors: Mi
authors: Department of Dermatology, Henry Ford Health System, Detroit, MI, USA.|Henry Ford Immunology Program, Henry Ford Health System, Detroit, MI, USA.|Department of Immunology and Microbiology, Wayne State University, Detroit, MI, USA.|Department of Internal Medicine, Henry Ford Health System, Detroit, MI, USA.
authors: Li
authors: L
authors: Zhou
mesh_terms: D000634:Aminoquinolines; D000818:Animals; D015536:Down-Regulation; D000077271:Imiquimod; D015603:Keratinocytes; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics*; D011565:Psoriasis / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D013601:T-Lymphocytes; D015854:Up-Regulation
chemical_list: D000634:Aminoquinolines; C575895:MIRN17-92 microRNA, mouse; C561642:MIRN19 microRNA, mouse; D035683:MicroRNAs; C543614:Mirn17 microRNA, mouse; C541873:Mirn92 microRNA, mouse; D000077271:Imiquimod
keywords: T cells; imiqumod; knockin; knockout; miRNAs; psoriasis