title: Controlled deposition of functionalized silica coated zinc oxide nano-assemblies at the air/water interface for blood cancer detection.
abstract: We report results of the studies relating to controlled deposition of the amino-functionalized silica-coated zinc oxide (Am-Si@ZnO) nano-assemblies onto an indium tin oxide (ITO) coated glass substrate using Langmuir-Blodgett (LB) technique. The monolayers have been deposited by transferring the spread solution of Am-Si@ZnO stearic acid prepared in chloroform at the air-water interface, at optimized pressure (16 mN/m), concentration (10 mg/ml) and temperature (23 °C). The high-resolution transmission electron microscopic studies of the Am-Si@ZnO nanocomposite reveal that the nanoparticles have a microscopic structure comprising of hexagonal assemblies of ZnO with typical dimensions of 30 nm. The surface morphology of the LB multilayer observed by scanning electron microscopy shows uniform surface of the Am-Si@ZnO film in the nanometer range (<80 nm). These electrodes have been utilized for chronic myelogenous leukemia (CML) detection by covalently immobilizing the amino-terminated oligonucleotide probe sequence via glutaraldehyde as a crosslinker. The response studies of these fabricated electrodes carried out using electrochemical impedance spectroscopy show that this Am-Si@ZnO LB film based nucleic acid sensor exhibits a linear response to complementary DNA (10(-6)-10(-16) M) with a detection limit of 1 × 10(-16) M. This fabricated platform is validated with clinical samples of CML positive patients and the results demonstrate its immense potential for clinical diagnosis.
authors: Biomedical Instrumentation Section, CSIR-National Physical Laboratory, New Delhi 110012, India; Nanobioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Delhi 110042, India.
authors: Chandra Mouli
authors: CM
authors: Pandey
authors: Biomedical Instrumentation Section, CSIR-National Physical Laboratory, New Delhi 110012, India; Biomedical Engineering Department, Deenbandhu Chhotu Ram University of Science & Technology, Haryana 131039, India.
authors: Srishti
authors: S
authors: Dewan
authors: Biomedical Engineering Department, Deenbandhu Chhotu Ram University of Science & Technology, Haryana 131039, India.
authors: Seema
authors: S
authors: Chawla
authors: Rajiv Gandhi Cancer Institute and Research Centre, Rohini, Delhi 110085, India.
authors: Birendra Kumar
authors: BK
authors: Yadav
authors: Biomedical Instrumentation Section, CSIR-National Physical Laboratory, New Delhi 110012, India. Electronic address: sumanagajjala@gmail.com.
authors: Gajjala
authors: G
authors: Sumana
authors: Biomedical Instrumentation Section, CSIR-National Physical Laboratory, New Delhi 110012, India; Nanobioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Delhi 110042, India. Electronic address: bansi.malhotra@gmail.com.
authors: Bansi Dhar
authors: BD
authors: Malhotra
mesh_terms: D000388:Air; D015374:Biosensing Techniques; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004566:Electrodes; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000175:diagnosis*; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D017971:Tin Compounds / Q000737:chemistry; D014867:Water / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D017971:Tin Compounds; D014867:Water; C109984:indium tin oxide; D012822:Silicon Dioxide; D015034:Zinc Oxide
keywords: Cancer; Chronic myelogenous leukemia; Langmuir-blodgett monolayer; Nucleic acid biosensor; Zinc oxide