title: Triadin and CLIMP-63 form a link between triads and microtubules in muscle cells.
abstract: In skeletal muscle, the triad is a structure comprising a transverse (T)-tubule and sarcoplasmic reticulum (SR) cisternae. Triads constitute the basis of excitation-contraction coupling as the cradle of the Ca2+release complex. We have shown previously that triadin, a member of this complex, has shaping properties on reticulum membrane and is indirectly involved in a link between triads and microtubules. We have identified here that CLIMP-63 (also known as CKAP4), as the partner of triadin, is responsible for this association of triads and microtubules. Triadin and CLIMP-63 interact through their respective luminal domains and the shaping properties of triadin depend on the capacity of CLIMP-63 to bind microtubules with its cytosolic portion. In skeletal muscle, CLIMP-63 is localized in the SR, including triads, and is associated with the Ca2+release complex through its interaction with triadin. Knockout of triadin in muscles results in the delocalization of CLIMP-63 from triads, its dissociation from the Ca2+release complex and a disorganization of the microtubule network. Our results suggest that the association of triadin and CLIMP-63 could be involved in the shaping of SR terminal cisternae and in the guidance of microtubules close to the triads.
authors: University Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, Grenoble F-38000, France INSERM, U1216, Grenoble F-38000, France.
authors: Alexis
authors: 0000-0003-3435-5785
authors: A
authors: Osseni
authors: University Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, Grenoble F-38000, France INSERM, U1216, Grenoble F-38000, France.
authors: Muriel
authors: M
authors: Sébastien
authors: University Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, Grenoble F-38000, France INSERM, U1216, Grenoble F-38000, France.
authors: Oriana
authors: 0000-0002-6992-8144
authors: O
authors: Sarrault
authors: University Grenoble Alpes, BIG-BGE, Grenoble F-38000, France CEA, BIG, BGE and GPC, Grenoble F-38000, France INSERM, BGE, Grenoble F-38000, France.
authors: Mathieu
authors: 0000-0001-8404-6070
authors: M
authors: Baudet
authors: University Grenoble Alpes, BIG-BGE, Grenoble F-38000, France CEA, BIG, BGE and GPC, Grenoble F-38000, France INSERM, BGE, Grenoble F-38000, France.
authors: Yohann
authors: Y
authors: Couté
authors: University Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, Grenoble F-38000, France INSERM, U1216, Grenoble F-38000, France CHU de Grenoble, Grenoble F-38000, France.
authors: Julien
authors: J
authors: Fauré
authors: University Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, Grenoble F-38000, France INSERM, U1216, Grenoble F-38000, France CEA, BIG, BGE and GPC, Grenoble F-38000, France isabelle.marty@univ-grenoble-alpes.fr anne.fourest-lieuvin@univ-grenoble-alpes.fr.
authors: Anne
authors: 0000-0003-1325-6788
authors: A
authors: Fourest-Lieuvin
authors: University Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, Grenoble F-38000, France INSERM, U1216, Grenoble F-38000, France isabelle.marty@univ-grenoble-alpes.fr anne.fourest-lieuvin@univ-grenoble-alpes.fr.
authors: Isabelle
authors: 0000-0003-3625-913X
authors: I
authors: Marty
mesh_terms: D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D002522:Chlorocebus aethiops; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D018345:Mice, Knockout; D008870:Microtubules / Q000378:metabolism*; D032342:Muscle Cells / Q000378:metabolism*; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding; D000072417:Protein Domains; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D014162:Transfection
chemical_list: C081876:CKAP4 protein, human; C558686:CLIMP-63 protein, mouse; D002352:Carrier Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D020033:Protein Isoforms; C498269:TRDN protein, human; C070048:triadin
keywords: CLIMP-63; Ca2+ release complex; Microtubule; Sarcoplasmic reticulum; Triad; Triadin