title: Characterization of a liposome-based artificial skin membrane for in vitro permeation studies using Franz diffusion cell device.
abstract: A prerequisite for successful transdermal or dermal drug therapy is the drug ability to penetration through the skin, especially stratum corneum (SC). The most acceptable technique for measuring skin permeation in vitro is the application of both the Franz diffusion cell device and the skin model. In the skin model, a liposome-based artificial skin membrane (LASM) consisting of tight layers of liposomes immobilized on a filter was prepared and characterized. Using porcine ear skin, rat skin and Strat-M™ artificial membrane as control, the LASM was then evaluated in permeation studies with five active compounds: ferulic acid, paeoniflorin, albiflorin, tetrahydrocolumbamine, and tetrahydropalmatine. The scanning electron microscope images demonstrated complete filling of the membrane pores with lipids and the formation of a continuous liposomal coating. The contents of egg phosphatidylcholine (EPC) and cholesterol in LASM were measured to be 12.08 ± 0.18 and 4.41 ± 0.04 mg/cm2, respectively. Moreover, revealed by the measurement of electrical resistance, the LASM remains intact for at least 12 h with the incubation of 20% ethanol. The results of permeation studies demonstrated a good correlation (r2= 0.9743, r = 0.9871) of Pappvalues between the drugs' permeation through LASM and porcine ear skin. In addition, by ATR-FTIR analysis, a slighter shift of CH2stretching frequency between LASM and porcine ear skin was observed compared with the shift between Strat-M™ membrane and porcine ear skin. In summary, for the first time, the LASM has been proved to be a valuable alternative to porcine ear skin in permeation studies using Franz diffusion cell device.
authors: a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine , Nanjing , China and.|b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.
authors: Hui
authors: H
authors: Zhang
authors: a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine , Nanjing , China and.|b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.
authors: Xuemin
authors: X
authors: Zhu
authors: b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.
authors: Jingjing
authors: J
authors: Shen
authors: b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.
authors: Haiheng
authors: H
authors: Xu
authors: a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine , Nanjing , China and.|b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.
authors: Min
authors: M
authors: Ma
authors: b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.
authors: Wei
authors: W
authors: Gu
authors: a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine , Nanjing , China and.|b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.
authors: Qiudong
authors: Q
authors: Jiang
authors: a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine , Nanjing , China and.|b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.
authors: Jun
authors: J
authors: Chen
authors: a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine , Nanjing , China and.
authors: Jinao
authors: J
authors: Duan
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002784:Cholesterol / Q000737:chemistry; D004058:Diffusion; D004817:Epidermis / Q000378:metabolism; D004864:Equipment and Supplies; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000138:chemical synthesis* / Q000378:metabolism*; D008567:Membranes, Artificial; D010539:Permeability; D010713:Phosphatidylcholines / Q000737:chemistry; D051381:Rats; D012869:Skin Absorption / Q000502:physiology*; D017768:Skin, Artificial; D013552:Swine
chemical_list: D008055:Lipids; D008081:Liposomes; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D002784:Cholesterol
keywords: Artificial skin membrane; liposomes; permeation studies