title: Taxonomy and physiological characterisation of Scheffersomyces titanus sp. nov., a new D-xylose-fermenting yeast species from China.
abstract: Three strains of a d-xylose-fermenting yeast species were isolated from the host beetle Dorcus titanus collected from two different localities in Henan Province, Central China. These strains formed two hat-shaped ascospores in conjugated and deliquescent asci. Multilocus phylogenetic analysis that included the nearly complete small subunit (SSU), the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit (LSU) rDNAs, as well as RNA polymerase II largest subunit (RPB1) gene demonstrated that these strains represent a novel yeast species belonging to the genus Scheffersomyces. The phylogenetic analysis based on the nucleotide sequences of the xylose reductase (XYL1) gene supported the view that the new strains could be grouped as a unique species. Although this new species is highly similar to Scheffersomyces stipitis-like yeasts in terms of nrDNA sequences and morphological and physiological characteristics, the species can be clearly differentiated from its close relatives on the basis of the sequences of XYL1 and RPB1. Therefore, a novel yeast species, Scheffersomyces titanus sp. nov., is proposed to accommodate these strains. The type strain is NYNU 14712(T) (CICC 33061(T) = CBS 13926(T)).
authors: School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P. R. China.
authors: Xiao-Jing
authors: XJ
authors: Liu
authors: School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P. R. China.
authors: Wan-Nan
authors: WN
authors: Cao
authors: School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P. R. China.
authors: Yong-Cheng
authors: YC
authors: Ren
authors: School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P. R. China.
authors: Long-Long
authors: LL
authors: Xu
authors: School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P. R. China.
authors: Ze-Hao
authors: ZH
authors: Yi
authors: School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P. R. China.
authors: Zheng
authors: Z
authors: Liu
authors: School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, P. R. China.|Henan Provincial Key Laboratory of Funiu Mountain Insect Biology, Nanyang Normal University, Nanyang 473061, P. R. China.
authors: Feng-Li
authors: FL
authors: Hui
mesh_terms: D000818:Animals; D002681:China; D001517:Coleoptera / Q000382:microbiology; D004275:DNA, Ribosomal; D005285:Fermentation; D010802:Phylogeny; D012319:RNA Polymerase II / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification* / Q000502:physiology*; D013172:Spores, Fungal; D014994:Xylose / Q000378:metabolism*
chemical_list: D004275:DNA, Ribosomal; D014994:Xylose; D012319:RNA Polymerase II