title: Evaluation of Genetic Diversity of Candida spp. and Klebsiella spp. Isolated from the Denture Plaque of COPD Patients.
abstract: Yeast-like fungi and gram-negative bacilli are the most frequent potential pathogens of the respiratory tract isolated from the denture plaque of patients with chronic obstructive pulmonary disease (COPD). Dominant species among yeast-like fungi are Candida albicans and Candida tropicalis. Significant frequency is also exhibited by Klebsiella pneumoniae and Klebsiella oxytoca. The purpose of this study was to analyze genetic diversity of the strains of C. albicans, C. tropicalis, and Klebsiella spp. present in patients in stable phases of COPD. The analysis was conducted by the random amplified polymorphic DNA (RAPD) method on clinical strains isolated from patients with COPD and control patients in overall good health. Forty one strains of Candida albicans, 12 of Candida tropicalis, as well as 9 strains of K. pneumoniae and 7 of K. oxytoca were scrutinized. The dominant species in clinical material from COPD patients was Candida albicans with a substantial degree of variations of genetic profiles. On the basis of affinity analysis, 19 genetic types were identified within this strain. An analysis of the banding patterns among C. tropicalis strains indicated the existence of 6 genetic types. A considerable diversity of genetic profiles among Klebsiella spp. also was established. The genotype diversity of Klebsiella spp. strains may indicate the endogenic character of the majority of infections, regardless of the therapy applied for the underlying condition.
authors: Department of Prosthodontics, Warsaw Medical University, 59 Nowogrodzka Street, 02-005, Warsaw, Poland. dorota.przybylowska@gmail.com.
authors: D
authors: D
authors: Przybyłowska
authors: Department of Medical Microbiology, Warsaw Medical University, Warsaw, Poland.
authors: K
authors: K
authors: Piskorska
authors: Department of Medical Microbiology, Warsaw Medical University, Warsaw, Poland.
authors: M
authors: M
authors: Gołaś
authors: Department of Dental Microbiology, Warsaw Medical University, Warsaw, Poland.
authors: M
authors: M
authors: Sikora
authors: Department of Medical Microbiology, Warsaw Medical University, Warsaw, Poland.
authors: E
authors: E
authors: Swoboda-Kopeć
authors: Department of Prosthodontics, Warsaw Medical University, 59 Nowogrodzka Street, 02-005, Warsaw, Poland.
authors: J
authors: J
authors: Kostrzewa-Janicka
authors: Department of Prosthodontics, Warsaw Medical University, 59 Nowogrodzka Street, 02-005, Warsaw, Poland.
authors: E
authors: E
authors: Mierzwińska-Nastalska
mesh_terms: D002175:Candida / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002176:Candida albicans / Q000235:genetics; D041022:Candida tropicalis / Q000235:genetics; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology*; D016022:Case-Control Studies; D004269:DNA, Bacterial / Q000235:genetics*; D003773:Dental Plaque / Q000175:diagnosis / Q000382:microbiology*; D014644:Genetic Variation; D006801:Humans; D007709:Klebsiella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D007710:Klebsiella Infections / Q000175:diagnosis / Q000382:microbiology*; D041121:Klebsiella oxytoca / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis / Q000382:microbiology* / Q000503:physiopathology; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D004269:DNA, Bacterial
keywords: COPD; Candida spp.; Denture plaque; Genotyping; Klebsiella spp.; Respiratory tract