title: Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo.
abstract: The activity of tankyrase (Tnks) enzymes modulates the activity of the β-catenin destruction complex in the Wnt/β-catenin signaling pathway. Here, we describe a method for determining the accessibility of various zebrafish tissues in vivo and in vitro to small molecule inhibitors of Tankyrase enzymes. This biochemical assay will facilitate chemically based studies focused on understanding the role of Tankyrase in cell fate reprogramming and tissue homeostasis and provide insights into the potential role of Wnt/β-catenin signaling in these processes.
authors: Pediatrics and Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors: Jesung
authors: J
authors: Moon
authors: Pediatrics, Molecular Biology and Internal Medicine, University of Texas Southwestern Medical Center, ND3.120C, 5323 Harry Hines Blvd., Dallas, TX, 75390-8543, USA. James.Amatruda@utsouthwestern.edu.
authors: James F
authors: JF
authors: Amatruda
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006706:Homeostasis / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D038501:Tankyrases / Q000737:chemistry* / Q000235:genetics; D060449:Wnt Signaling Pathway; D015027:Zebrafish / Q000235:genetics; D051176:beta Catenin / Q000737:chemistry* / Q000235:genetics
chemical_list: D004791:Enzyme Inhibitors; D051176:beta Catenin; D038501:Tankyrases
keywords: Tankyrase; Western blotting; Wnt inhibitor; Wnt/β-catenin signaling; Zebrafish