title: Immunofluorescent Staining of Mouse Intestinal Stem Cells.
abstract: Immunofluorescent staining of organoids can be performed to visualize molecular markers of cell behavior. For example, cell proliferation marked by incorporation of nucleotide (EdU), or to observe markers of intestinal differentiation including paneth cells, goblet cells, or enterocytes (see Figure 1). In this protocol we detail a method to fix, permeabilize, stain and mount intestinal organoids for analysis by immunofluorescent confocal microscopy.
authors: Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program, New York, USA; Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, USA.
authors: Kevin P
authors: KP
authors: O'Rourke
authors: Department of Medicine, Weill Cornell Medical College, New York, USA.
authors: Lukas E
authors: LE
authors: Dow
authors: Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, USA; Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, USA.
authors: Scott W
authors: SW
authors: Lowe