title: A wheat COP9 subunit 5-like gene is negatively involved in host response to leaf rust.
abstract: The COP9 (constitutive photomorphogenesis 9) signalosome (CSN) is a protein complex involved in the ubiquitin proteasome system and a common host target of diverse pathogens in Arabidopsis. The known derubylation function of the COP9 complex is carried out by subunit 5 encoded by AtCSN5A or AtCSN5B in Arabidopsis. A single CSN5-like gene (designated as TaCSN5) with three homeologues was identified on the long arms of wheat (Triticum aestivum L.) group 2 chromosomes. In this study, we identified and characterized the function of TaCSN5 in response to infection by the leaf rust pathogen. Down-regulation of all three TaCSN5 homeologues or mutations in the homeologues on chromosomes 2A or 2D resulted in significantly enhanced resistance to leaf rust. Enhanced leaf rust resistance corresponded to a seven-fold increase in PR1 (pathogenesis-related gene 1) expression. Collectively, the data indicate that the wheat COP9 subunit 5-like gene acts as a negative regulator of wheat leaf rust resistance.
authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, 59717-3150, USA.
authors: Hongtao
authors: H
authors: Zhang
authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, 59717-3150, USA.|State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shanxi, 712100, China.
authors: Xiaojing
authors: X
authors: Wang
authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, 59717-3150, USA.
authors: Michael J
authors: MJ
authors: Giroux
authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, 59717-3150, USA.
authors: Li
authors: L
authors: Huang
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D001487:Basidiomycota / Q000502:physiology*; D003001:Cloning, Molecular; D003198:Computer Simulation; D005020:Ethyl Methanesulfonate; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D054884:Host-Pathogen Interactions / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D014908:Triticum / Q000235:genetics* / Q000382:microbiology*
chemical_list: D010940:Plant Proteins; D021122:Protein Subunits; D005020:Ethyl Methanesulfonate
keywords: Triticum aestivum; VIGS; in silico cloning; leaf rust; qRT-PCR