title: Guanylate-Binding Protein-1 protects ovarian cancer cell lines but not breast cancer cell lines from killing by paclitaxel.
abstract: Forced expression of the cytokine-induced large GTPase, human Guanylate-Binding Protein-1 (hGBP-1), in ovarian cancer cell lines increases resistance to paclitaxel. Elevated hGBP-1 RNA in ovarian tumors correlates with shorter recurrence-free survival. In contract, hGBP-1 is part of a gene signature predicting improved prognosis in all subtypes of breast cancers. hGBP-1 does not confer paclitaxel resistance on MCF-7 and TMX2-28 breast cancer cells. Expression of the isotype of the hGBP-1-interacting protein, PIM1, which may contribute to paclitaxel resistance when associated with hGBP-1, is different in breast and ovarian cancer cell lines. Breast cancer cell lines express the 44 kDa isoform of PIM-1, and ovarian cancer cell lines express the 33 kDa isoform.
authors: Department of Biological Sciences, University of Toledo, 2801 West Bancroft St., Toledo, OH 43606, Spain.
authors: Aaron R
authors: AR
authors: Tipton
authors: Department of Biological Sciences, University of Toledo, 2801 West Bancroft St., Toledo, OH 43606, Spain.
authors: Geoffrey O
authors: GO
authors: Nyabuto
authors: Department of Biological Sciences, University of Toledo, 2801 West Bancroft St., Toledo, OH 43606, Spain.
authors: Jill A
authors: JA
authors: Trendel
authors: Department of Medicine, University of Toledo, 3000 Arlington St., Toledo, OH 43614, Spain.
authors: Travis M
authors: TM
authors: Mazur
authors: Department of Biological Sciences, University of Toledo, 2801 West Bancroft St., Toledo, OH 43606, Spain.
authors: John P
authors: JP
authors: Wilson
authors: Department of Biological Sciences, University of Toledo, 2801 West Bancroft St., Toledo, OH 43606, Spain.
authors: Suzan
authors: S
authors: Wadi
authors: Department of Biological Sciences, University of Toledo, 2801 West Bancroft St., Toledo, OH 43606, Spain.
authors: Jacob S
authors: JS
authors: Justinger
authors: Department of Biological Sciences, University of Toledo, 2801 West Bancroft St., Toledo, OH 43606, Spain.
authors: Garret L
authors: GL
authors: Moore
authors: Department of Medicine, University of Toledo, 3000 Arlington St., Toledo, OH 43614, Spain.
authors: Peter T
authors: PT
authors: Nguyen
authors: Department of Biological Sciences, University of Toledo, 2801 West Bancroft St., Toledo, OH 43606, Spain. Electronic address: Deborah.Vestal@utoledo.edu.
authors: Deborah J
authors: DJ
authors: Vestal
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D019610:Cytoprotection / Q000187:drug effects; D018572:Disease-Free Survival; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology*; D017239:Paclitaxel / Q000494:pharmacology*; D051573:Proto-Oncogene Proteins c-pim-1 / Q000378:metabolism; D064726:Triple Negative Breast Neoplasms / Q000473:pathology; D014404:Tubulin / Q000378:metabolism
chemical_list: C490287:GBP1 protein, human; C498585:TUBB3 protein, human; D014404:Tubulin; C496634:PIM1 protein, human; D051573:Proto-Oncogene Proteins c-pim-1; D019204:GTP-Binding Proteins; D017239:Paclitaxel
keywords: Breast cancer; Drug resistance; Guanylate-binding protein; Paclitaxel