title: Enhanced expression of semaphorin 3E is involved in the gastric cancer development.
abstract: Semaphorins and their receptors are abnormally expressed in various cancers, but little is known about the expression and function of semaphorin 3E (SEMA3E) and its receptor, plexin D1 (PLXND1), in gastric cancer development or metastasis. We evaluated SEMA3E and PLXND1 expression by quantitative RT-PCR in gastric tissues from 62 patients who underwent gastrectomy and analyzed the correlation between their expression and clinicopathological variables. To assess the function of SEMA3E, we generated human gastric cancer cell lines with suppressed or increased SEMA3E expression. The expression level of SEMA3E, but not PLXND1, was correlated with lymph node involvement and metastatic progression in gastric cancer. A significant association was observed between a high level of SEMA3E expression and poor differentiation or poor survival in the intestinal type of gastric cancer. SEMA3E knockdown in gastric cancer cells attenuated cell proliferation and metastatic ability in vitro and in vivo. Moreover, SEMA3E caused cell proliferation and anchorage-independent cell growth in the intestinal type of gastric cancer. These results suggested that SEMA3E is likely to be involved in the development of gastric cancer and might also be a therapeutic target for its treatment.
authors: Division of Cancer Stem Cell, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan.
authors: Ryuhei
authors: R
authors: Maejima
authors: Division of Cancer Stem Cell, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan.
authors: Keiichi
authors: K
authors: Tamai
authors: Division of Cancer Stem Cell, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan.
authors: Takeharu
authors: T
authors: Shiroki
authors: Division of Cancer Stem Cell, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan.
authors: Misa
authors: M
authors: Yokoyama
authors: Division of Cancer Stem Cell, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan.
authors: Rie
authors: R
authors: Shibuya
authors: Division of Cancer Stem Cell, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan.
authors: Mao
authors: M
authors: Nakamura
authors: Division of Molecular and Cellular Oncology, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan.
authors: Kazunori
authors: K
authors: Yamaguchi
authors: Division of Cancer Stem Cell, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan.
authors: Makoto
authors: M
authors: Abue
authors: Department of Gastroenterology, Miyagi Cancer Center, Natori, Miyagi, Japan.
authors: Tomoyuki
authors: T
authors: Oikawa
authors: Department of Gastroenterology, Miyagi Cancer Center, Natori, Miyagi, Japan.
authors: Tetsuya
authors: T
authors: Noguchi
authors: Department of Gastroenterological Surgery, Miyagi Cancer Center, Natori, Miyagi, Japan.
authors: Koh
authors: K
authors: Miura
authors: Department of Gastroenterological Surgery, Miyagi Cancer Center, Natori, Miyagi, Japan.
authors: Tsuneaki
authors: T
authors: Fujiya
authors: Department of Pathology, Miyagi Cancer Center, Natori, Miyagi, Japan.
authors: Ikuro
authors: I
authors: Sato
authors: Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.
authors: Katsunori
authors: K
authors: Iijima
authors: Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.
authors: Tooru
authors: T
authors: Shimosegawa
authors: Division of Cancer Biology and Therapeutics, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan.
authors: Nobuyuki
authors: N
authors: Tanaka
authors: Division of Cancer Stem Cell, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan.
authors: Kennichi
authors: K
authors: Satoh
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008562:Membrane Glycoproteins; D051379:Mice; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D011379:Prognosis; D039961:Semaphorins / Q000235:genetics* / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016019:Survival Analysis; D015854:Up-Regulation
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; C493082:PLXND1 protein, human; C494123:SEMA3E protein, human; D039961:Semaphorins