title: Harvest tissue source does not alter the protective power of stromal cell therapy after intestinal ischemia and reperfusion injury.
abstract: Transplantation of mesenchymal stromal cells (MSCs) may be a novel treatment for intestinal ischemia. The optimal stromal cell source that could yield maximal protection after injury, however, has not been identified. We hypothesized that (1) MSCs would increase survival and mesenteric perfusion, preserve intestinal histologic architecture, and limit inflammation after intestinal ischemia and reperfusion (I/R) injury, and (2) MSCs harvested from different sources of tissue would have equivalent protective properties to the intestine after I/R inury.
  
  
  
  Adult male mice were anesthetized, and a midline laparotomy was performed. The intestines were eviscerated, the small bowel mesenteric root was identified, and baseline intestinal perfusion was determined using laser Doppler imaging. Intestinal ischemia was established by temporarily occluding the superior mesenteric artery for 60 min with a noncrushing clamp. After ischemia, the clamp was removed and the intestines were allowed to recover. Before abdominal closure, 2 × 10(6) human umbilical cord-derived MSCs, bone marrow-derived MSCs, or keratinocytes in 250 μL of phosphate-buffered saline vehicle were injected into the peritoneum. Animals were allowed to recover for 12 or 24 h (perfusion, histology, and inflammatory studies) or 7 d (survival studies). Survival data was analyzed using the log-rank test. Perfusion was expressed as a percentage of the baseline, and 12- and 24-h data was analyzed using one-way analysis of variance and the Student t-test. Nonparametric data was compared using the Mann-Whitney U-test. A P value of <0.05 was considered statistically significant.
  
  
  
  All MSCs increased 7-d survival after I/R injury and were superior to vehicle and keratinocytes (P < 0.05). All MSCs increased mesenteric perfusion more than vehicle at 12 and 24 h after injury (P < 0.05). All MSCs provided superior perfusion compared with keratinocytes at 24 h after injury (P < 0.05). Administration of each MSC line improved intestinal histology after I/R injury (P < 0.05). Multiple proinflammatory chemokines were downregulated after the application of MSCs, suggesting a decreased inflammatory response after MSC therapy.
  
  
  
  Transplantation of MSCs after intestinal I/R injury, irrespective of a tissue source, significantly increases survival and mesenteric perfusion and at the same time limits intestinal damage and inflammation. Further studies are needed to identify the mechanism that these cells use to promote improved outcomes after injury.
authors: Department of Surgery, Section of Pediatric Surgery, Indianapolis, Indiana; Indiana University School of Medicine, Indianapolis, Indiana.
authors: Amanda R
authors: AR
authors: Jensen
authors: Department of Surgery, Section of Pediatric Surgery, Indianapolis, Indiana; Indiana University School of Medicine, Indianapolis, Indiana.
authors: Morenci M
authors: MM
authors: Manning
authors: Department of Surgery, Section of Pediatric Surgery, Indianapolis, Indiana.
authors: Sina
authors: S
authors: Khaneki
authors: Department of Surgery, Section of Pediatric Surgery, Indianapolis, Indiana; Indiana University School of Medicine, Indianapolis, Indiana.
authors: Natalie A
authors: NA
authors: Drucker
authors: Department of Surgery, Section of Pediatric Surgery, Indianapolis, Indiana; Indiana University School of Medicine, Indianapolis, Indiana; Riley Hospital for Children at Indiana University Health, Indianapolis, Indiana. Electronic address: tmarkel@iupui.edu.
authors: Troy A
authors: TA
authors: Markel
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007410:Intestinal Diseases / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000098:blood supply* / Q000473:pathology; D015603:Keratinocytes; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D015427:Reperfusion Injury / Q000150:complications / Q000473:pathology / Q000517:prevention & control*; D013152:Splanchnic Circulation; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D016207:Cytokines
keywords: Human bone marrow mesenchymal stromal cells; Human umbilical mesenchymal stromal cells; Inflammation; Intestinal ischemia; Mortality; Perfusion; Survival