title: FgLPMO9A from Fusarium graminearum cleaves xyloglucan independently of the backbone substitution pattern.
abstract: Lytic polysaccharide monooxygenases (LPMOs) are important for the enzymatic conversion of biomass and seem to play a key role in degradation of the plant cell wall. In this study, we characterize an LPMO from the fungal plant pathogen Fusarium graminearum (FgLPMO9A) that catalyzes the mixed C1/C4 oxidative cleavage of cellulose and xyloglucan, but is inactive toward other (1,4)-linked β-glucans. Our findings indicate that FgLPMO9A has unprecedented broad specificity on xyloglucan, cleaving any glycosidic bond in the β-glucan main chain, regardless of xylosyl substitutions. Interestingly, we found that when incubated with a mixture of xyloglucan and cellulose, FgLPMO9A efficiently attacks the xyloglucan, whereas cellulose conversion is inhibited. This suggests that removal of hemicellulose may be the true function of this LPMO during biomass conversion.
authors: Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, Kongens Lyngby, Denmark.
authors: Laura
authors: L
authors: Nekiunaite
authors: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway.
authors: Dejan M
authors: DM
authors: Petrović
authors: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway.
authors: Bjørge
authors: B
authors: Westereng
authors: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway.
authors: Gustav
authors: G
authors: Vaaje-Kolstad
authors: Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, Kongens Lyngby, Denmark.
authors: Maher Abou
authors: MA
authors: Hachem
authors: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway. aniko.varnai@nmbu.no.
authors: Anikó
authors: A
authors: Várnai
authors: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway.
authors: Vincent G H
authors: VG
authors: Eijsink
mesh_terms: D002482:Cellulose / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D005670:Fusarium / Q000201:enzymology*; D005936:Glucans / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D014990:Xylans / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D014990:Xylans; C029353:xyloglucan; D002482:Cellulose; D006899:Mixed Function Oxygenases
keywords: 
  Fusarium graminearum
  ; AA9; cellulose; lytic polysaccharide monooxygenase; xyloglucan