title: A characteristic biosignature for discrimination of gastric cancer from healthy population by high throughput GC-MS analysis.
abstract: Early diagnosis of gastric cancer is crucial to improve patient' outcome. A good biomarker will function in early diagnosis for gastric cancer. In order to find practical and cost-effective biomarkers, we used gas chromatography combined mass spectrometer (GC-MS) to profile urinary metabolites on 293 urine samples. Ninety-four samples are taken as training set, others for validating study. Orthogonal partial least squares discriminant analysis (OPLS-DA), significance analysis of microarray (SAM) and Mann-Whitney U test are used for data analysis. The diagnostic value of urinary metabolites was evaluated by ROC curve. As results, Seventeen metabolites are significantly different between patients and healthy controls in training set. Among them, 14 metabolites show diagnostic value better than classic blood biomarkers by quantitative assay on validation set. Ten of them are amino acids and four are organic metabolites. Importantly, proline, p-cresol and 4-hydroxybenzoic acid disclose outcome-prediction value by means of survival analysis. Therefore, the examination of urinary metabolites is a promising noninvasive strategy for gastric cancer screening.
authors: Department of Surgery of Ruijin Hospital, and Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Yinan
authors: Y
authors: Chen
authors: Department of Surgery of Ruijin Hospital, and Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Jun
authors: J
authors: Zhang
authors: Department of Surgery of Ruijin Hospital, and Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Lei
authors: L
authors: Guo
authors: Department of Surgery of Ruijin Hospital, and Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Lei
authors: L
authors: Liu
authors: Tongji University, School of Life Science and Technology, Shanghai, China.
authors: Jingran
authors: J
authors: Wen
authors: Department of Surgery of Ruijin Hospital, and Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Lu
authors: L
authors: Xu
authors: Department of Surgery of Ruijin Hospital, and Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Min
authors: M
authors: Yan
authors: Tongji University, School of Life Science and Technology, Shanghai, China.
authors: Zuofeng
authors: Z
authors: Li
authors: Tongji University, School of Life Science and Technology, Shanghai, China.
authors: Xiaoyan
authors: X
authors: Zhang
authors: School of Life Sciences, Fudan University, Shanghai, China.
authors: Peng
authors: P
authors: Nan
authors: Department of Surgery of Ruijin Hospital, and Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Jinling
authors: J
authors: Jiang
authors: Department of Surgery of Ruijin Hospital, and Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Jun
authors: J
authors: Ji
authors: Department of Surgery of Ruijin Hospital, and Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Jianian
authors: J
authors: Zhang
authors: Department of Surgery of Ruijin Hospital, and Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Wei
authors: W
authors: Cai
authors: School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, China.
authors: Huisheng
authors: H
authors: Zhuang
authors: College of Public Health, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors: Yan
authors: Y
authors: Wang
authors: Department of Surgery of Ruijin Hospital, and Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Zhenggang
authors: Z
authors: Zhu
authors: Department of Surgery of Ruijin Hospital, and Shanghai Institute of Digestive Surgery, Shanghai Key Laboratory for Gastric Neoplasms, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Yingyan
authors: Y
authors: Yu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000596:Amino Acids / Q000652:urine; D014408:Biomarkers, Tumor / Q000652:urine; D055088:Early Detection of Cancer; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008297:Male; D053858:Metabolic Networks and Pathways; D008875:Middle Aged; D012372:ROC Curve; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000401:mortality / Q000652:urine
chemical_list: D000596:Amino Acids; D014408:Biomarkers, Tumor
keywords: GC-MS; biomarkers; gastric cancer; metabolomics; urine