title: The Effect of Ozone Therapy on Experimental Vasospasm in the Rat Femoral Artery.
abstract: OBJECTIVE
  Oxidation products following subarachnoid hemorrhage (SAH) are among the causative substances of cerebral vasospasm and poor outcome. Ozone (O3) is a gas that contains three atoms of oxygen with a cyclic structure. It has been suggested that application of low-dose ozone has an antioxidant effect and provides resistance to oxidative stress. We investigated the effect of oxygen-ozone therapy on rat femoral artery vasospasm.
  
  
  METHODS
  Twenty-four male Sprague-Dawley rats were randomly separated into vasospasm, vasospasm + ozone and control groups. The femoral artery vasospasm model was used. Rats in the vasospasm + ozone group were given 4 mL of ozone (20 μ/mL) daily for 7 days. Femoral arteries were examined by light microscopy for histological changes and morphometric analysis. Kruskal Wallis test and Mann Whitney U tests were used for the statistical analysis. The values of p < 0.01 and p < 0.05 were recognized as statistically significant.
  
  
  RESULTS
  Ozone treatment reduced the morphometric changes as irregularity of the elastic lamina, disruption of the endothelial cells, vacuolization and hemorrhages that caused by vasospasm. The measurements of the wall thickness (p=0.003; p < 0.01) and lumen diameter (p=0.001; p < 0.01) showed statistically significant difference (p < 0.01) between the vasospasm and vasospasm+ozone groups.
  
  
  CONCLUSIONS
  Ozone therapy may be useful in the treatment of post-hemorrhagic vasospasm.
authors: Haydarpasa Numune Teaching and Research Hospital, Department of Neurosurgery, Istanbul, Turkey.
authors: Metin
authors: M
authors: Orakdogen
authors: Serap
authors: S
authors: Uslu
authors: Selin Tural
authors: ST
authors: Emon
authors: Hakan
authors: H
authors: Somay
authors: Zeynep Cingu
authors: ZC
authors: Meric
authors: Tayfun
authors: T
authors: Hakan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D005263:Femoral Artery / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010126:Ozone / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020301:Vasospasm, Intracranial / Q000188:drug therapy*
chemical_list: D000975:Antioxidants; D010126:Ozone