title: Panoramic view of human corneal endothelial cell layer observed by a prototype slit-scanning wide-field contact specular microscope.
abstract: OBJECTIVE
  To observe the most peripheral region of the corneal endothelial cell (CEC) layer as long as optically recordable by use of a prototype slit-scanning wide-field contact specular microscope and produce a panoramic image to evaluate the variation of CEC density with ageing.
  
  
  METHODS
  Observational case series study.
  
  
  METHODS
  This study involved 15 eyes of 15 normal healthy subjects divided into three groups according to age: A (20-40 years), B (41-60 years) and C (>60 years). The corneal endothelial layer of each eye was recorded in a horizontal direction, from nasal to temporal, with a slit-scanning wide-field contact specular microscope (Konan) and endothelial cell density (ECD) in three specific regions (central, mid-peripheral, and peripheral) was automatically calculated via built-in analysis software.
  
  
  RESULTS
  Corneal endothelial images from near the surgical limbus to limbus in all eyes were clearly recorded and panoramic images were made by combining still images. ECD in groups A, B and C were 2809±186, 2717±91 and 2580±129 cells/mm2at the centre, 2902±242, 2772±97 and 2604±187 cells/mm2at the mid-periphery and 2893±308, 2691±99 and 2533±112 cells/mm2at the periphery. Significance differences in ECD was found between groups A and C in all regions and groups between B and C at mid-peripheral region.
  
  
  CONCLUSIONS
  A prototype slit-scanning wide-field contact specular microscope enabled us to record the endothelial layer from the surgical limbus to limbus of the cornea and compare specific areas among subjects, and showed that ECD in each region of the cornea decreases with ageing.
  
  
  BACKGROUND
  UMIN000021264, Results.
authors: Department of Ophthalmology, Kyoto Prefectural University of Medicine, Graduate School of Medicine, Kyoto, Japan.
authors: Hiroshi
authors: H
authors: Tanaka
authors: Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan.
authors: Naoki
authors: N
authors: Okumura
authors: Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan.
authors: Noriko
authors: N
authors: Koizumi
authors: Department of Ophthalmology, Kyoto Prefectural University of Medicine, Graduate School of Medicine, Kyoto, Japan.
authors: Chie
authors: C
authors: Sotozono
authors: Konan Medical, Inc, Nishinomiya, Japan.
authors: Yasuhiro
authors: Y
authors: Sumii
authors: Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors: Shigeru
authors: S
authors: Kinoshita
mesh_terms: D000328:Adult; D000368:Aged; D002452:Cell Count; D042783:Endothelial Cells / Q000166:cytology*; D004728:Endothelium, Corneal / Q000166:cytology*; D005260:Female; D006801:Humans; D008297:Male; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D008875:Middle Aged; D055815:Young Adult
keywords: Cornea; Diagnostic tests/Investigation; Imaging