title: RNA-seq analysis revealed ROS-mediated related genes involved in cadmium detoxification in the razor clam Sinonovacula constricta.
abstract: The razor clam Sinonovacula constricta is a eukaryotic benthic intertidal bivalve species that is tolerant to different heavy metals, such as cadmium ion (Cd(2+)). However, the mechanism by which S. constricta responds to Cd(2+)-induced stress remains unclear. In this study, eight transcriptome libraries were constructed and sequenced using razor clams exposed to Cd(2+) for 12 and 48 h. A total of 18,330 unigenes with an average length of 500 bp were annotated. Among these 18,330 unigenes, 582 and 649 displayed differential expression profiles at 12 and 48 h in the gill, respectively. The corresponding differential unigenes in the hepatopancreas were 1056 and 382. Gene Ontology annotation revealed that these unigenes were highly pronounced in metabolic process, cellular process, binding, and catalytic activity. Notably, ROS production-related genes, such as heat shock proteins 32, metallothionein, and glutathione, were synchronously enriched in all experimental samples with induced expression profiles, which was also validated by qPCR. Our results highlighted the relation between immune regulation and Cd(2+)-induced stress in razor clam and provided new insights into the molecular mechanisms of heavy toxicology.
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors: Zhenhui
authors: Z
authors: Wang
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors: Yina
authors: Y
authors: Shao
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China. Electronic address: lichenghua@nbu.edu.cn.
authors: Chenghua
authors: C
authors: Li
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors: Weiwei
authors: W
authors: Zhang
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors: Xuemei
authors: X
authors: Duan
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors: Xuelin
authors: X
authors: Zhao
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors: Qiongfen
authors: Q
authors: Qiu
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
authors: Chunhua
authors: C
authors: Jin
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000235:genetics* / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism*; D008658:Inactivation, Metabolic; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D002104:Cadmium
keywords: Cadmium; Glutathione; Heat shock protein32; Metallothionein; RNA-seq; Sinonovacula constricta