title: High-fat diet induced changes in lumbar vertebra of the male rat offsprings.
abstract: In obesity, bone marrow adiposity increases and proinflammatory cytokines excretion activates RANK/RANKL/OPG system, which leads to increased bone resorption. The aim of this study was to analyze trabecular and cortical bone parameters in animals exposed to the high-fat diet in utero and after lactation. Skeletal organ of interest was the fifth lumbar vertebra, which is not exposed to biomechanical loading in rats. Further aims were to determine TNF-α and IL-6 serum concentrations, and the intensity of the TNF-α immunohistochemical staining in the bone marrow. Ten female Sprague Dawley rats, nine weeks old, were randomly divided in two groups and fed either standard laboratory chow or food rich in saturated fatty acids during five weeks, and then mated with genetically similar male subjects. After birth and lactation male offsprings from both groups were divided in four subgroups depending on the diet they were fed until twenty-two weeks of age. The highest cholesterol and triglyceride concentration were found in both groups of offsprings fed with high-fat diet. The lowest trabecular bone volume, lowest trabecular number and highest trabecular separation were found in offsprings fed with high-fat diet of mothers on standard laboratory chow. The same group of offsprings was also characterized by the highest intensity of TNF-α immunostaining in the bone marrow and the highest TNF-α serum concentration, which suggest that this proinflammatory cytokine has interfered with bone metabolism, possibly by stimulation of bone resorption, which led to inadequate trabecular bone development and bone modeling of the fifth lumbar vertebra.
authors: Department of Anatomy and Neuroscience, Faculty of Medicine University of Osijek, J. Huttlera 4, 3100 Osijek, Croatia. Electronic address: zperic@mefos.hr.
authors: Zeljka
authors: Z
authors: Peric Kacarevic
authors: Department of Anatomy and Neuroscience, Faculty of Medicine University of Osijek, J. Huttlera 4, 3100 Osijek, Croatia; Clinical Institute of Nuclear Medicine and Radiation Protection, University Hospital Centre Osijek, J. Huttlera 4, 31000, Osijek, Croatia. Electronic address: dsnajder@mefos.hr.
authors: Darija
authors: D
authors: Snajder
authors: Department of Anatomy and Neuroscience, Faculty of Medicine University of Osijek, J. Huttlera 4, 3100 Osijek, Croatia; Department of Physical Medicine and Rehabilitation, University Hospital Centre Osijek, Suncana 37A, 31222 Bizovac, Croatia. Electronic address: angela.maric@gmail.com.
authors: Andela
authors: A
authors: Maric
authors: Department of Histology and Embryology, Faculty of Medicine, University of Osijek, J. Huttlera 4, 31000 Osijek, Croatia. Electronic address: nbijelic@mefos.hr.
authors: Nikola
authors: N
authors: Bijelic
authors: Department of Anatomy, Rijeka Medical Faculty, Brace Branchetta 20/1, 51000 Rijeka, Croatia. Electronic address: olga.cvijanovic@medri.uniri.hr.
authors: Olga
authors: O
authors: Cvijanovic
authors: Department of Chemistry and Biochemistry, Medical Faculty of University of Rijeka, Brace Branchetta 20/1, 51000 Rijeka, Croatia. Electronic address: robert.domitrovic@medri.uniri.hr.
authors: Robert
authors: R
authors: Domitrovic
authors: Department of Anatomy and Neuroscience, Faculty of Medicine University of Osijek, J. Huttlera 4, 3100 Osijek, Croatia. Electronic address: rradic@mefos.hr.
authors: Radivoje
authors: R
authors: Radic
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology*; D001862:Bone Resorption / Q000378:metabolism*; D001842:Bone and Bones / Q000378:metabolism*; D059305:Diet, High-Fat; D005260:Female; D015850:Interleukin-6 / Q000378:metabolism; D008159:Lumbar Vertebrae / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000378:metabolism*; D053245:RANK Ligand / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D053245:RANK Ligand; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha
keywords: Bone histomorphometry; Lumbar spine; Maternal obesity; Saturated fatty acids; TNF-α