title: First report of Sapajus cay naturally infected by Trypanosoma cruzi in San Pedro Department, Paraguay.
abstract: To verify the occurrence of natural Trypanosoma cruzi infection in non-human primates from a rural endemic area of the east region of Paraguay, xenodiagnosis was performed in 35 animals belonging to two species. For genotyping and T. cruzi discrete typing unit (DTU) assignment, a combination of four markers was used, including amplification products of the small (18S) and large (24Sα) subunits of ribosomal ribonucleic acid gene, the intergenic region of mini-exon gene and the heat shock protein 60 Eco-RV polymerase chain reaction-restriction fragment length polymorphism (HSP60/EcoRV-PCR-RFLP). One specimen of Sapajus cay was found positive and infected by the DTU TcII. This result constitutes the first record of natural T. cruzi infection in a sylvatic monkey in Paraguay, harbouring a DTU associated with severe Chagas disease in humans.
authors: Departamento de Medicina Tropical, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción - UNA, San Lorenzo, Paraguay.
authors: Nidia
authors: N
authors: Acosta
authors: Departamento de Medicina Tropical, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción - UNA, San Lorenzo, Paraguay.
authors: Jorge
authors: J
authors: Miret
authors: Departamento de Medicina Tropical, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción - UNA, San Lorenzo, Paraguay.
authors: Elsa
authors: E
authors: López
authors: Departamento de Medicina Tropical, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción - UNA, San Lorenzo, Paraguay.
authors: Alicia
authors: A
authors: Schinini