title: Quantitative index imaging of coculture cells by scanning focused refractive index microscopy.
abstract: We report the quantitative refractive index (RI) imaging of cocultured cells in their living environment by scanning focused refractive index microscopy (SFRIM). Mouse microglial cells and synovial cells are cocultured on the top surface of a trapezoid prism. The RI imaging of living cells is obtained in a reflection-type method. The RI information is deduced with the simple derivative total internal reflection method, where a complex retrieval algorithm or reconstruction process is unnecessary. The outline of each cell is determined according to the RI value compared with that of the immersion liquid. The cocultured cells can be discriminated in the RI image. The measurement is nondestructive and label-free. The experimental results prove that SFRIM is a promising tool in the field of biological optics.
authors: Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, China.
authors: Teng-Qian
authors: TQ
authors: Sun
authors: Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, ChinabNankai University, The 2011 Project Collaborative Innovation Center for Biological Therapy, 94th Weijin Road, Tianjin 300071, China.
authors: Qing
authors: Q
authors: Ye
authors: Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, China.
authors: Fen
authors: F
authors: Hu
authors: Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, China.
authors: Shi-Ke
authors: SK
authors: Liu
authors: Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, China.
authors: Xiao-Wan
authors: XW
authors: Wang
authors: Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, China.
authors: Jin
authors: J
authors: Wang
authors: Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, China.
authors: Zhi-Chao
authors: ZC
authors: Deng
authors: Nankai University, The 2011 Project Collaborative Innovation Center for Biological Therapy, 94th Weijin Road, Tianjin 300071, ChinacNankai University, Advanced Technology Institute, 94th Weijin Road, Tianjin 300071, China.
authors: Jian-Chun
authors: JC
authors: Mei
authors: Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, ChinabNankai University, The 2011 Project Collaborative Innovation Center for Biological Therapy, 94th Weijin Road, Tianjin 300071, China.
authors: Wen-Yuan
authors: WY
authors: Zhou
authors: Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, China.
authors: Chun-Ping
authors: CP
authors: Zhang
authors: Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, China.
authors: Xin-Yu
authors: XY
authors: Wang
authors: Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, ChinadNankai University, State Key Laboratory of Medicinal Chemical Biology, 94th Weijin Road, Tianjin, China.
authors: Lei-Ting
authors: LT
authors: Pan
authors: Nankai University, School of Physics and TEDA Applied Physics School, Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, 94th Weijin Road, Tianjin 300071, ChinabNankai University, The 2011 Project Collaborative Innovation Center for Biological Therapy, 94th Weijin Road, Tianjin 300071, China.
authors: Jian-Guo
authors: JG
authors: Tian
mesh_terms: D000818:Animals; D018920:Coculture Techniques; D051379:Mice; D017628:Microglia / Q000166:cytology*; D008853:Microscopy; D061848:Optical Imaging / Q000379:methods*; D012031:Refractometry; D013582:Synovial Fluid / Q000166:cytology*