title: Matrine Exerts a Strong Anti-Arthritic Effect on Type II Collagen-Induced Arthritis in Rats by Inhibiting Inflammatory Responses.
abstract: To investigate anti-arthritic effects of matrine isolated from the roots of S. flavescens on type II collagen-induced arthritis (CIA) in rats and to explore its related potential mechanisms, CIA rats were established and administered with matrine (20, 40 or 80 mg/kg/days, for 30 days). Subsequently, blood was collected to determine serum levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, IL-10, MMP-2, MMP-3 and MMP-9, and hind paws and knee joints were collected for histopathological examination. Furthermore, indices of the thymus and spleen were determined, and synovial tissues were collected to determine the protein expressions of p-IκB, IκB, Cox-2 and iNOS. Our results indicated that matrine significantly suppressed inflammatory reactions and synovial tissue destruction. Matrine inhibited paw swelling, arthritis indices and weight loss in CIA rats. Additionally, matrine decreased the levels of TNF-α, IL-1β, IL-6, IL-8, IL-17A, MMP-2, MMP-3 and MMP-9. Matrine also down-regulated expressions of p-IκB, Cox-2, and iNOS but up-regulated IκB in synovial tissues in CIA rats. The results suggested matrine possesses an anti-arthritic effect in CIA rats via inhibiting the release of pro-inflammatory cytokines and proteins that promote the NF-κB pathway.
authors: Administrative Office, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China. pujiang0408@163.com.
authors: Jiang
authors: J
authors: Pu
authors: Department of Rehabilitation, Changhai Hospital of TCM, Second Military Medical University, Shanghai 200433, China. fangfanfu@126.com.
authors: Fan-Fu
authors: FF
authors: Fang
authors: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China. liuxiuqing@126.com.
authors: Xiu-Qing
authors: XQ
authors: Li
authors: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China. 13817760547@163.com.
authors: Zhi-Heng
authors: ZH
authors: Shu
authors: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China. msjyp@163.com.
authors: Yi-Ping
authors: YP
authors: Jiang
authors: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China. than927@163.com.
authors: Ting
authors: T
authors: Han
authors: College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China. pengwei002@126.com.
authors: Wei
authors: W
authors: Peng
authors: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China. zhengchengjian1984@126.com.
authors: Cheng-Jian
authors: CJ
authors: Zheng
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D024043:Collagen Type II / Q000633:toxicity*; D007249:Inflammation / Q000097:blood / Q000188:drug therapy*; D016753:Interleukin-10 / Q000097:blood; D020381:Interleukin-17 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood; D019278:Matrix Metalloproteinase 3 / Q000097:blood; D020780:Matrix Metalloproteinase 9 / Q000097:blood; D011807:Quinolizines / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D029909:Sophora / Q000737:chemistry; D000093842:Matrines
chemical_list: D000470:Alkaloids; D024043:Collagen Type II; D020381:Interleukin-17; D015850:Interleukin-6; D016209:Interleukin-8; D011807:Quinolizines; D016753:Interleukin-10; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D000093842:Matrines
keywords: Sophora flavescens; anti-arthritic effect; matrine; rheumatoid arthritis; type II collagen