title: Inhibitory Effects of Vegetable Juices on CYP3A4 Activity in Recombinant CYP3A4 and LS180 Cells.
abstract: It is thought that eating habits induces individual variation in intestinal absorption and metabolism of drugs. The objective of this research was to clarify the influence of vegetables juices on CYP3A4 activity, which is an important enzyme in intestine. Five vegetables juices (VJ-o, Kagome Original(®); VJ-g, Kagome 30 kinds of vegetables and fruits(®); VJ-p, Kagome Purple vegetables(®); VJ-r, Kagome Sweet Tomato(®); and VJ-y, Kagome Fruity Salada(®); KAGOME Co., Ltd., Aichi, Japan) were centrifuged (1630×g, 10 min) and filtered using filter paper and 0.45-µm membrane filters. In this study, recombinant CYP3A4 and LS180 cells were used for the evaluation of CYP3A4 activity. The metabolisms to 6β-hydroxytestosterone by recombinant CYP3A4 were significantly inhibited by VJ-o, VJ-g, and VJ-y in a preincubation time-dependent manner, and CYP3A4 activity in LS180 cells were significantly inhibited by VJ-o and VJ-y. These results show that the difference in ingestion volume of vegetable juices and vegetables might partially induce individual difference in intestinal drug metabolism.
authors: Department of Clinical Pharmacy, Faculty of Pharmaceutical Science, Kyoto Pharmaceutical University.
authors: Masayuki
authors: M
authors: Tsujimoto
authors: Tomoe
authors: T
authors: Uchida
authors: Hiroyuki
authors: H
authors: Kozakai
authors: Saori
authors: S
authors: Yamamoto
authors: Tetsuya
authors: T
authors: Minegaki
authors: Kohshi
authors: K
authors: Nishiguchi
mesh_terms: D045744:Cell Line, Tumor; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism*; D018565:Food-Drug Interactions; D000067030:Fruit and Vegetable Juices; D006801:Humans; D006915:Hydroxytestosterones / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014675:Vegetables
chemical_list: D006915:Hydroxytestosterones; D011994:Recombinant Proteins; C025591:6 beta-hydroxytestosterone; D051544:Cytochrome P-450 CYP3A