title: Differentially expressed genes in heads and tails of Angelica sinensis diels: Focusing on ferulic acid metabolism.
abstract: OBJECTIVE
  To explore the scientific connotation of the discrepant pharmaceutical activities between the head and tail of Angelica sinensis diels (AS), an important herb extensively utilized in Chinese medicine, by the approach of transcriptome sequencing.
  
  
  METHODS
  Ten samples of AS were randomly collected in Min County, Gansu Province of China. Transcriptome sequencing of AS was accomplished in a commercial ILLumina HiSeq-2000 platform. The transcriptome of each head and tail of AS were fixed in a gene chip, and detected under the procedure of Illumina HiSeq-2000. Differentially expressed unigenes between the heads and tails of AS were selected by Shanghai Biotechnology Corporation (SBC) online analysis system, based on Gene Ontology, Kyoto Encyclopedia of Genes and Genomes and relevant bioinformatic database.
  
  
  RESULTS
  Totally 63,585 unigenes were obtained from AS by high-throughput sequencing platform. Among which 3359 unigenes were identified as differentially expressed unigenes between the heads and tails of AS by SBC analysis system scanning. Of which 15 differentially expressed unigenes participate in the metabolic regulation of phenylpropanoid biosynthesis (PB) pathway and ferulic acid metabolites, in response to the distinguished pharmaceutical actions of the heads and tails of AS.
  
  
  CONCLUSIONS
  Different content of ferulic acid in the heads and tails of AS is related to the differentially expressed genes, particularly involved in the PB pathway.
authors: Bijie Medical College, Bijie, Guizhou Province, 551700, China.|Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Jie
authors: J
authors: Yang
authors: Bijie Medical College, Bijie, Guizhou Province, 551700, China.|Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Wei-Hong
authors: WH
authors: Li
authors: Bijie Medical College, Bijie, Guizhou Province, 551700, China.|Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Rong
authors: R
authors: An
authors: Bijie Medical College, Bijie, Guizhou Province, 551700, China.|Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Yi-Li
authors: YL
authors: Wang
authors: Bijie Medical College, Bijie, Guizhou Province, 551700, China.|Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Yan
authors: Y
authors: Xu
authors: Bijie Medical College, Bijie, Guizhou Province, 551700, China.|Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Jie
authors: J
authors: Chen
authors: Bijie Medical College, Bijie, Guizhou Province, 551700, China.|Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Xiao-Fang
authors: XF
authors: Wang
authors: Bijie Medical College, Bijie, Guizhou Province, 551700, China.|Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Xiao-Bo
authors: XB
authors: Zhang
authors: Bijie Medical College, Bijie, Guizhou Province, 551700, China.|Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Jing
authors: J
authors: Li
authors: Bijie Medical College, Bijie, Guizhou Province, 551700, China. Dingwj123@163.com.|Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China. Dingwj123@163.com.
authors: Wei-Jun
authors: WJ
authors: Ding
mesh_terms: D029971:Angelica sinensis / Q000235:genetics* / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D003373:Coumaric Acids / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D053858:Metabolic Networks and Pathways / Q000235:genetics; D058977:Molecular Sequence Annotation; D020005:Propanols / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics
chemical_list: D003373:Coumaric Acids; D020005:Propanols; C439395:1-phenylpropanol; C004999:ferulic acid
keywords: Angelica sinensis diels; Chinese medicine; ferulic acid; phenylpropanoid biosynthesis pathway; transcriptome sequencing