title: Reduced Expression of SARM in Mouse Spleen during Polymicrobial Sepsis.
abstract: Objective Immune dysfunction, including prominent apoptosis of immune cells and decreased functioning of the remaining immune cells, plays a central role in the pathogenesis of sepsis. Sterile α and HEAT/armadillo motif-containing protein (SARM) is implicated in the regulation of immune cell apoptosis. This study aimed to elucidate SARM contributes to sepsis-induced immune cell death and immunosuppression. Methods A mouse model of polymicrobial sepsis was generated by cecum ligation and puncture (CLP). SARM gene and protein expression, caspase 3 cleavage and intracellular ATP production were measured in the mouse spleens. Results CLP-induced polymicrobial sepsis specifically attenuated both the gene and protein expression of SARM in the spleens. Moreover, the attenuation of SARM expression synchronized with splenocyte apoptosis, as evidenced by increased caspase 3 cleavage and ATP depletion. Conclusions These findings suggest that SARM is a potential regulator of sepsis-induced splenocyte apoptosis.
authors: Biomedicine Research Center and Department of Surgery, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Guangzhou, Guangdong, China, 510150. shirley_gong17@hotmail.com.|Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. shirley_gong17@hotmail.com.
authors: Yu
authors: Y
authors: Gong
authors: Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
authors: Lin
authors: L
authors: Zou
authors: Department of Oncology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors: Dongzhi
authors: D
authors: Cen
authors: Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
authors: Wei
authors: W
authors: Chao
authors: Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, 63 Duobao Road, Guangzhou, Guangdong, China, 510150. djchen_gysy@sina.com.
authors: Dunjin
authors: D
authors: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology; D051186:Armadillo Domain Proteins / Q000378:metabolism*; D060085:Coinfection / Q000276:immunology*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004195:Disease Models, Animal; D007108:Immune Tolerance; D051379:Mice; D018805:Sepsis / Q000276:immunology*; D013154:Spleen / Q000378:metabolism* / Q000473:pathology
chemical_list: D051186:Armadillo Domain Proteins; D003598:Cytoskeletal Proteins; C543961:SARM1 protein, mouse
keywords: SARM; polymicrobial sepsis; splenocyte apoptosis