title: Quantitative Proteomics Analysis of Leukemia Cells.
abstract: Chronic myeloid leukemia (CML) is driven by the oncogenic fusion kinase Bcr-Abl, which organizes its own signaling network with various proteins. These proteins, their interactions, and their role in relevant signaling pathways can be analyzed by quantitative mass spectrometry (MS) approaches in various models systems, e.g., in cell culture models. In this chapter, we describe in detail immunoprecipitations and quantitative proteomics analysis using stable isotope labeling by amino acids in cell culture (SILAC) of components of the Bcr-Abl signaling pathway in the human CML cell line K562.
authors: Institute of Molecular Medicine and Cell Research (IMMZ), Faculty of Medicine, University of Freiburg, Stefan Meier Strasse 17, 79104, Freiburg im Breisgau, Germany.|Faculty of Biology, University of Freiburg, Freiburg im Breisgau, Germany.|Spemann Graduate School of Biology and Medicine, University of Freiburg, Freiburg im Breisgau, Germany.
authors: Sebastian
authors: S
authors: Halbach
authors: BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg im Breisgau, Germany. joern.dengjel@medizin.uni-freiburg.de.|FRIAS Freiburg Institute for Advanced Studies, University of Freiburg, Freiburg im Breisgau, Germany. joern.dengjel@medizin.uni-freiburg.de.|Department of Dermatology, University Medical Center, University of Freiburg, Freiburg im Breisgau, Germany. joern.dengjel@medizin.uni-freiburg.de.|ZBSA Center for Biological Systems Analysis, University of Freiburg, Freiburg im Breisgau, Germany. joern.dengjel@medizin.uni-freiburg.de.
authors: Jörn
authors: J
authors: Dengjel
authors: Institute of Molecular Medicine and Cell Research (IMMZ), Faculty of Medicine, University of Freiburg, Stefan Meier Strasse 17, 79104, Freiburg im Breisgau, Germany. tilman.brummer@zbsa.uni-freiburg.de.|Faculty of Biology, University of Freiburg, Freiburg im Breisgau, Germany. tilman.brummer@zbsa.uni-freiburg.de.|BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg im Breisgau, Germany. tilman.brummer@zbsa.uni-freiburg.de.|Deutsches Konsortium für Translationale Krebsforschung (DKTK) and Comprehensive Cancer Center Freiburg, University Medical Center, Freiburg im Breisgau, Germany. tilman.brummer@zbsa.uni-freiburg.de.
authors: Tilman
authors: T
authors: Brummer
mesh_terms: D018929:Cell Culture Techniques; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D047468:Immunoprecipitation; D007553:Isotope Labeling; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry / Q000379:methods*
keywords: Bcr-Abl; Chronic myeloid leukemia; Gab2; Immunoprecipitation; Mass spectrometry; Posttranslational modification; Protein complex; Protein dynamics; Proteomics; SILAC