title: A Photoswitchable Fluorophore for the Real-Time Monitoring of Dynamic Events in Living Organisms.
abstract: This study reports the synthesis of a photoactivatable fluorophore with optimal photochemical and photophysical properties for the real-time tracking of motion in vivo. The photoactivation mechanism designed into this particular compound permits the conversion of an emissive reactant into an emissive product with resolved fluorescence, under mild illumination conditions that are impossible to replicate with conventional switching schemes based on bleaching. Indeed, the supramolecular delivery of these photoswitchable probes into the cellular blastoderm of Drosophila melanogaster embryos allows the real-time visualization of translocating molecules with no detrimental effects on the developing organisms. Thus, this innovative mechanism for fluorescence photoactivation can evolve into a general chemical tool to monitor dynamic processes in living biological specimens.
authors: Laboratory for Molecular Photonics, Departments of Biology and Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL, 33146-0431, USA.
authors: Yang
authors: Y
authors: Zhang
authors: Laboratory for Molecular Photonics, Departments of Biology and Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL, 33146-0431, USA.
authors: Sicheng
authors: S
authors: Tang
authors: Laboratory for Molecular Photonics, Departments of Biology and Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL, 33146-0431, USA.
authors: Lorenzo
authors: L
authors: Sansalone
authors: Laboratory for Molecular Photonics, Departments of Biology and Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL, 33146-0431, USA.
authors: James D
authors: JD
authors: Baker
authors: Laboratory for Molecular Photonics, Departments of Biology and Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL, 33146-0431, USA. fraymo@miami.edu.
authors: Françisco M
authors: FM
authors: Raymo
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster; D005456:Fluorescent Dyes / Q000737:chemistry*; D055668:Photochemical Processes
chemical_list: D005456:Fluorescent Dyes
keywords: BODIPY; molecular switches; photoactivatable fluorophores; photocages; supramolecular nanocarriers