title: Citrus flavanones naringenin and hesperetin improve antioxidant status and membrane lipid compositions in the liver of old-aged Wistar rats.
abstract: This study aimed to investigate effects of citrus flavanones naringenin (NAR) and hesperetin (HES) on liver antioxidant status and membrane phospholipid composition in 24-month-old rats. NAR and HES (15mg/kg) were administrated orally to male Wistar rats, once per day, for 4weeks. Control group received either vehicle (sunflower oil) or remained intact. The results showed decreased (p<0.05) activity of antioxidant enzymes (AOE), specifically catalase (CAT), superoxide dismutase (SOD) 1 and glutathione reductase (GR) in the liver of intact control old-aged rats in comparison to young intact controls. Flavanone administration to old-aged males increased (p<0.05) examined AOE activities in comparison to vehicle-administered animals. Namely, NAR was more potent in comparison to HES regarding the increase (p<0.05) in activities of examined antioxidant enzymes (SOD 1 and 2, glutathione peroxidase-GPx and GR) and the liver glutathione (GSH), while HES elevated (p<0.05) only activity of CAT and GR. Both flavanones significantly decreased (p<0.05) TBARS and improved (p<0.05) membrane phospholipid composition in favor of n-3 PUFA and n-6/n-3 PUFA ratio. Both flavanones did not affect liver histology and reduced (p<0.05) alanine aminotransferase and aspartate aminotransferase levels in serum. The results of this study indicate beneficial potential of citrus flavanones in the old-aged rat liver.
authors: Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia. Electronic address: marko.miler@ibiss.bg.ac.rs.
authors: Marko
authors: M
authors: Miler
authors: Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
authors: Jasmina
authors: J
authors: Živanović
authors: Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
authors: Vladimir
authors: V
authors: Ajdžanović
authors: Department of Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
authors: Zorana
authors: Z
authors: Oreščanin-Dušić
authors: INRA, UMR 1019, UNH, CRNH Auvergne, Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Dragan
authors: D
authors: Milenković
authors: Centre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, University of Belgrade, Belgrade, Serbia.
authors: Aleksandra
authors: A
authors: Konić-Ristić
authors: Department of Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
authors: Duško
authors: D
authors: Blagojević
authors: Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
authors: Verica
authors: V
authors: Milošević
authors: Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
authors: Branka
authors: B
authors: Šošić-Jurjević
mesh_terms: D000375:Aging / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002957:Citrus / Q000737:chemistry*; D044950:Flavanones / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006569:Hesperidin / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010743:Phospholipids / Q000737:chemistry; D010938:Plant Oils; D051381:Rats; D017208:Rats, Wistar; D000074242:Sunflower Oil; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000975:Antioxidants; D044950:Flavanones; D010743:Phospholipids; D010938:Plant Oils; D000074242:Sunflower Oil; D017392:Thiobarbituric Acid Reactive Substances; D006569:Hesperidin; C005273:naringenin; C013015:hesperetin
keywords: Antioxidant system; Citrus flavanones; Liver; Old age rats; Phospholipid composition