title: A population study using the human erythrocyte PIG-A assay.
abstract: Erythrocyte-based PIG-A assay is sensitive and reliable in detecting exposure to mutagenetic agents in animal studies, but there are few data from human populations. In this study, we employed a method for detecting CD59 phenotypic variants, resulting from mutation in the PIG-A gene, in human red blood cells (RBCs), and determined the CD59-deficient RBC (RBCCD59-) frequencies in 217 subjects from general population. The majority of subjects had a relatively low mutant frequencies (MFs) (average, 5.25 ± 3.6 × 10-6, median, 4.38 × 10-6, for all subjects), but with males having a significantly greater MFs (5.97 ± 4.0 × 10-6) than females (4.19 ± 2.5 ×10-6). There was no correlation between MFs and age. In addition, MFs showed no difference between smoker and nonsmoker, and also no association with smoke duration in male subjects. However, there was a significant correlation between cigarette-pack-years which indicated that the MF was only slightly elevated with the increase of cigarette-pack-years. Moreover, intraindividual variations were investigated in three volunteer subjects over 300 days, and the MFs were relatively stable and repeatable. Furthermore, a pilot study by using white blood cell (WBC) assay based on labeling with FLAER was performed in volunteer subjects. The MFs of FLAER-deficient WBC (WBCFLAER-) and RBCCD59-were consistently elevated in two subjects. Our findings provide baseline data that will be helpful in designing further studies using the PIG-A assay to monitor the genotoxic effects of carcinogens in human populations. Environ. Mol. Mutagen. 57:589-604, 2016. © 2016 Wiley Periodicals, Inc.
authors: Hongqiao International Institute of Medicine, Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
authors: Yiyi
authors: Y
authors: Cao
authors: Department of Hematology, Tong Ren Hospital Affiliated to Shanghai Jiaotong University School Of Medicine, Shanghai, 200336, China.
authors: Li
authors: L
authors: Yang
authors: Hongqiao International Institute of Medicine, Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
authors: Nannan
authors: N
authors: Feng
authors: Hongqiao International Institute of Medicine, Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
authors: Oumin
authors: O
authors: Shi
authors: Hongqiao International Institute of Medicine, Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
authors: Jing
authors: J
authors: Xi
authors: Hongqiao International Institute of Medicine, Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
authors: Xinyue
authors: X
authors: You
authors: Department of Hematology, Tong Ren Hospital Affiliated to Shanghai Jiaotong University School Of Medicine, Shanghai, 200336, China.
authors: Chunrong
authors: C
authors: Yin
authors: Department of Endocrinology, Tong Ren Hospital Affiliated to Shanghai Jiaotong University School Of Medicine, Shanghai, 200336, China.
authors: Huan
authors: H
authors: Yang
authors: Division of Genetics and Mutagenesis, National Institute of Health Sciences, Setagaya-ku, Tokyo, 158-8501, Japan.
authors: Katsuyoshi
authors: K
authors: Horibata
authors: Division of Genetics and Mutagenesis, National Institute of Health Sciences, Setagaya-ku, Tokyo, 158-8501, Japan.
authors: Masamitsu
authors: M
authors: Honma
authors: Hongqiao International Institute of Medicine, Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
authors: Biyun
authors: B
authors: Qian
authors: Department of Hematology, Tong Ren Hospital Affiliated to Shanghai Jiaotong University School Of Medicine, Shanghai, 200336, China. yluan@sjtu.edu.cn.
authors: Wei
authors: W
authors: Weng
authors: Hongqiao International Institute of Medicine, Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China. chenwengw@163.com.
authors: Yang
authors: Y
authors: Luan
mesh_terms: D018957:CD59 Antigens / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D005828:Genetics, Population; D064368:Healthy Volunteers; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D059645:Mutation Rate
chemical_list: D018957:CD59 Antigens; D008565:Membrane Proteins; D009153:Mutagens; C080086:phosphatidylinositol glycan-class A protein
keywords: PIG-A assay; flow cytometer; human population; red blood cells; somatic mutation