title: Methods for Detecting Microbial Methane Production and Consumption by Gas Chromatography.
abstract: Methane is an energy-dense fuel but is also a greenhouse gas 25 times more detrimental to the environment than CO2. Methane can be produced abiotically by serpentinization, chemically by Sabatier or Fisher-Tropsh chemistry, or biotically by microbes (Berndtet al., 1996; Horita and Berndt, 1999; Dry, 2002; Wolfe, 1982; Thauer, 1998; Metcalfet al., 2002). Methanogens are anaerobic archaea that grow by producing methane gas as a metabolic byproduct (Wolfe, 1982; Thauer, 1998). Our lab has developed and optimized three different gas chromatograph-utilizing assays to characterize methanogen metabolism (Catlettet al., 2015). Here we describe the end point and kinetic assays that can be used to measure methane production by methanogens or methane consumption by methanotrophic microbes. The protocols can be used for measuring methane production or consumption by microbial pure cultures or by enrichment cultures.
authors: Department of Biochemistry, Redox Biology Center, N200 Beadle Center, University of Nebraska-Lincoln, Lincoln, USA.
authors: Jared T
authors: JT
authors: Aldridge
authors: Department of Biochemistry, Redox Biology Center, N200 Beadle Center, University of Nebraska-Lincoln, Lincoln, USA.
authors: Jennie L
authors: JL
authors: Catlett
authors: Department of Biochemistry, Redox Biology Center, N200 Beadle Center, University of Nebraska-Lincoln, Lincoln, USA.
authors: Megan L
authors: ML
authors: Smith
authors: Department of Biochemistry, Redox Biology Center, N200 Beadle Center, University of Nebraska-Lincoln, Lincoln, USA.
authors: Nicole R
authors: NR
authors: Buan