title: Development of a simple, low-cost and eurytopic medium based on Pleurotus eryngii for lactic acid bacteria.
abstract: Lactic acid bacteria (LAB) are a group of important beneficial microorganisms for human, but their growth is restricted to the habitats with rich nutrients. In order to develop a simple, low-cost and efficient medium based on the mushroom Pleurotus eryngii, this study evaluated the effects of different treatment methods for the mushroom, concentration of the mushroom, buffers, tween 80, MgSO4·7H2O, MnSO4·4H2O, CuSO4·5H2O, riboflavin and ascorbic acid on the growth of Lactococcus lactis subsp. lactis SLPE1-3. An optimized medium was developed, which was composed of the mushroom at 200 g/L, the buffer sodium acetate at 5 g/L, and riboflavin at 0.5 mg/L. The mushroom was ground, boiled and filtered for the filtrate in advance. In this optimized medium which was named as PSR medium, the population density of SLPE1-3 sharply reached 2.13 × 10(9) CFU/mL within 18 h of incubation, and still maintained 1.17 × 10(8) CFU/mL at 120 h. In addition, this study found that 6 kinds of LAB could grow almost well, and maintained high survival in PSR medium compared to M17 or MRS medium, including Lactococcus lactis subsp. lactis, Lactobacillus plantarum, Lactococcus lactis subsp. cremoris, Lactobacillus paracasei, Pediococcus pentosaceus and Lactobacillus rhamnosus. These results showed that PSR medium was a simple, low cost and eurytopic medium for the cultivation of LAB, and could replace MRS or M17 medium in the food industry, biomedicine and laboratory.
authors: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors: Yancun
authors: Y
authors: Zhao
authors: Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors: Ying
authors: Y
authors: Wang
authors: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors: Zhiwei
authors: Z
authors: Song
authors: Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors: Chengjun
authors: C
authors: Shan
authors: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
authors: Runjie
authors: R
authors: Zhu
authors: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. fqliu20011@sina.com.
authors: Fengquan
authors: F
authors: Liu
keywords: Lactic acid bacteria; Nutrient requirements; Pleurotus eryngii; Riboflavin; Sodium acetate