title: Characterization of a Rolling-Circle Replication Plasmid pM411 from Lactobacillus plantarum 1-3.
abstract: A cryptic plasmid pM411 isolated from Lactobacillus plantarum 1-3 consisted of a 2303-bp circular molecule with a G + C content 32.96 %. Sequence analysis of pM411 revealed four putative open reading frames (ORFs). ORF1 shared 99 and 94 % similarities, respectively, with the Rep proteins of plasmids pLC2 and pYC2, which belong to the rolling-circle replication pMV158 family. A typical pMV158 family double-strand origin (dso) and a putative single-strand origin (sso) located upstream of the rep gene. Southern hybridization confirmed the presence of single-strand DNA (ssDNA) intermediates, suggesting that pM411 belongs to the RCR pMV158 family. Sequence homology analysis indicated that the sso belongs to the ssoW family. Furthermore, the relative copy number of pM411 was about 88 copies in each cell by real-time PCR.
authors: Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, Beijing, 100083, People's Republic of China.
authors: Xvchuan
authors: X
authors: Ma
authors: Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, Beijing, 100083, People's Republic of China.
authors: Jinhua
authors: J
authors: Li
authors: Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, Beijing, 100083, People's Republic of China.
authors: Yao
authors: Y
authors: Xiong
authors: Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, Beijing, 100083, People's Republic of China.
authors: Zhengyuan
authors: Z
authors: Zhai
authors: The Innovation Centre of Food Nutrition and Human Health (Beijing), College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.
authors: Fazheng
authors: F
authors: Ren
authors: The Innovation Centre of Food Nutrition and Human Health (Beijing), College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China. haoyl@cau.edu.cn.|Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, Beijing, 100083, People's Republic of China. haoyl@cau.edu.cn.
authors: Yanling
authors: Y
authors: Hao
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D048191:Lactiplantibacillus plantarum / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial