title: DNA forms of arboviral RNA genomes are generated following infection in mosquito cell cultures.
abstract: Although infections of vertebrate hosts by arthropod-borne viruses may lead to pathogenic outcomes, infections of vector mosquitoes result in persistent infections, where the virus replicates in the host without causing apparent pathological effects. It is unclear how persistent infections are established and maintained in mosquitoes. Several reports revealed the presence of flavivirus-like DNA sequences in the mosquito genome, and recent studies have shown that DNA forms of RNA viruses restrict virus replication in Drosophila, suggesting that DNA forms may have a role in developing persistent infections. Here, we sought to investigate whether arboviruses generate DNA forms following infection in mosquitoes. Our results with West Nile, Dengue, and La Crosse viruses demonstrate that DNA forms of the viral RNA genome are generated in mosquito cells; however, not the entire viral genome, but patches of viral RNA in DNA forms can be detected 24h post infection.
authors: Wadsworth Center, Griffin Laboratory, New York State Department of Health, 5668 State Farm Road, Slingerlands, NY 12159, USA; Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, NY 12201, USA. Electronic address: dilip.nag@health.ny.gov.
authors: Dilip K
authors: DK
authors: Nag
authors: Wadsworth Center, Griffin Laboratory, New York State Department of Health, 5668 State Farm Road, Slingerlands, NY 12159, USA. Electronic address: matthew.brecher@health.ny.gov.
authors: Matthew
authors: M
authors: Brecher
authors: Wadsworth Center, Griffin Laboratory, New York State Department of Health, 5668 State Farm Road, Slingerlands, NY 12159, USA; Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, NY 12201, USA. Electronic address: laura.kramer@health.ny.gov.
authors: Laura D
authors: LD
authors: Kramer
mesh_terms: D000818:Animals; D001103:Arboviruses / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D009033:Culicidae / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D023061:Gene Order; D016679:Genome, Viral; D016366:Open Reading Frames; D014779:Virus Replication
chemical_list: D004279:DNA, Viral
keywords: Arbovirus; Bunyavirus; Dengue virus; Flavivirus; La Crosse virus; Mosquito vectors; Persistent infections; West Nile virus