title: A multiplex allele-specific real-time polymerase chain reaction assay for HLA-B*13:01 genotyping in four Chinese populations.
abstract: Human leukocyte antigen HLA-B*13:01 is identified currently as a marker of individual susceptibility to drug-induced hypersensitivity reaction, such as dapsone-induced hypersensitivity reactions (DIHRs) and trichloroethylene-induced dermatitis. Therefore, screening for the HLA-B*13:01 allele can assist clinics in identifying patients at risk of developing DIHRs. By combining the allele-specific primers with TaqMan probes, we established a single tube, triplex real-time PCR to detect HLA-B*13:01. The reliability of this assay was validated by the comparison of genotyping results with those by sequence-based typing (SBT). With this assay, the distribution of HLA-B*13:01 in a total of 350 blood samples from four ethnic groups: Han, Tibetan, Uighur, and Buyei were determined. A 100% concordance was observed between the results with the established real-time PCR and SBT in 100 samples. The detection limit of this assay was 0.016 ng genomic DNA. The prevalence of HLA-B*13:01 carriers were 11%, 8%, 1%, and 2% in the Buyei (n = 100), Northern Han (n = 100), Tibetan (n = 100), and Uighur (n = 50) populations, respectively. The multiplex real-time PCR assay provided a fast and reliable method for accurate detection of HLA-B*13:01 allele prior to dapsone administration in clinical practice and onset of the reaction after exposure to trichloroethylene.
authors: National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University, Xi'an, China.
authors: Z
authors: Z
authors: Liu
authors: Gynecology Laboratory, Xi an GaoXin Hospital, Xi'an, China.
authors: G
authors: G
authors: Chen
authors: National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University, Xi'an, China.
authors: X
authors: X
authors: Kang
authors: National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University, Xi'an, China.
authors: M
authors: M
authors: Han
authors: National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University, Xi'an, China.
authors: R
authors: R
authors: Chen
authors: National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University, Xi'an, China. cchen898@nwu.edu.cn.
authors: C
authors: C
authors: Chen
authors: National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University, Xi'an, China. whj@nwu.edu.cn.
authors: H
authors: H
authors: Wang
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002681:China; D017931:DNA Primers / Q000138:chemical synthesis / Q000378:metabolism; D015342:DNA Probes / Q000138:chemical synthesis / Q000378:metabolism; D003622:Dapsone / Q000008:administration & dosage; D004342:Drug Hypersensitivity / Q000097:blood / Q000175:diagnosis* / Q000208:ethnology / Q000276:immunology; D005006:Ethnicity; D005091:Exons; D005787:Gene Frequency; D059908:HLA-B13 Antigen / Q000145:classification / Q000235:genetics* / Q000276:immunology; D064368:Healthy Volunteers; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007438:Introns; D057230:Limit of Detection; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*; D017422:Sequence Analysis, DNA; D014241:Trichloroethylene / Q000008:administration & dosage
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D059908:HLA-B13 Antigen; D014241:Trichloroethylene; D003622:Dapsone
keywords: HLA-B*13:01; dapsone-induced hypersensitivities; multiplex real-time PCR; trichloroethylene-induced dermatitis