title: A preliminary investigation on the relationship between gut microbiota and gene expressions in peripheral mononuclear cells of infants with autism spectrum disorders.
abstract: Fecal and blood samples of infants with autism spectrum disorders (ASD) and healthy infants were analyzed to investigate the association of altered gut microbiota and ASD development. 16S rRNA gene-based sequencing found that, unlike those of healthy infants, feces of ASD infants had significantly higher and lower abundance of genera Faecalibacterium and Blautia, respectively. Moreover, DNA microarray analysis of peripheral blood mononuclear cells (PBMC) detected more highly than low expressed genes in ASD infants than in healthy infants. Gene Ontology analysis revealed that differentially expressed genes between ASD and healthy infants were involved in interferon (IFN)-γ and type-I IFN signaling pathways. Finally, strong positive correlations between expression of IFN signaling-associated genes in PBMC and fecal abundance of Faecalibacterium were found. Our results strongly suggested that altered gut microbiota in infants resulted from ASD development and was associated with systemic immunity dysregulation, especially chronic inflammation.
authors: a Laboratory of Animal Science, Department of Agricultural and Life Sciences , Kyoto Prefectural University , Kyoto , Japan.
authors: Ryo
authors: R
authors: Inoue
authors: b Department of Pediatrics , Shiga University of Medical Science , Otsu , Japan.
authors: Yuko
authors: Y
authors: Sakaue
authors: b Department of Pediatrics , Shiga University of Medical Science , Otsu , Japan.
authors: Chihiro
authors: C
authors: Sawai
authors: b Department of Pediatrics , Shiga University of Medical Science , Otsu , Japan.
authors: Toshihiro
authors: T
authors: Sawai
authors: c Department of Informatics and Mediology , Mukogawa Women's University , Nishinomiya , Japan.
authors: Motoyuki
authors: M
authors: Ozeki
authors: d Kyoto Institute of Nutrition and Pathology , Kyoto , Japan.
authors: Gustavo A
authors: GA
authors: Romero-Pérez
authors: a Laboratory of Animal Science, Department of Agricultural and Life Sciences , Kyoto Prefectural University , Kyoto , Japan.|d Kyoto Institute of Nutrition and Pathology , Kyoto , Japan.
authors: Takamitsu
authors: T
authors: Tsukahara
mesh_terms: D000067877:Autism Spectrum Disorder / Q000097:blood / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D016022:Case-Control Studies; D002675:Child, Preschool; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome; D006801:Humans; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D059467:Transcriptome
keywords: Faecalibacterium; autism spectrum disorders; gut microbiota; interferon signaling; peripheral blood mononuclear cell