title: RNAi Screening in Spodoptera frugiperda.
abstract: RNA interference is a potent and precise reverse genetic approach to carryout large-scale functional genomic studies in a given organism. During the past decade, RNAi has also emerged as an important investigative tool to understand the process of viral pathogenesis. Our laboratory has successfully generated transgenic reporter and RNAi sensor line of Spodoptera frugiperda (Sf21) cells and developed a reversal of silencing assay via siRNA or shRNA guided screening to investigate RNAi factors or viral pathogenic factors with extraordinary fidelity. Here we describe empirical approaches and conceptual understanding to execute successful RNAi screening in Spodoptera frugiperda 21-cell line.
authors: Insect Resistance Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors: Subhanita
authors: S
authors: Ghosh
authors: Insect Resistance Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.|College of Veterinary Medicine, Veterinary Medicine Academic Building, 1900 Coffey Road, Columbus, OH, 43210, USA.
authors: Gatikrushna
authors: G
authors: Singh
authors: Insect Resistance Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors: Bindiya
authors: B
authors: Sachdev
authors: Centre for Bioinformatics, M.D. University, Rohtak, 124001, India.
authors: Ajit
authors: A
authors: Kumar
authors: Malaria Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India. pawanmal@gmail.com.
authors: Pawan
authors: P
authors: Malhotra
authors: Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India. sunilmukherjeeudsc@gmail.com.
authors: Sunil K
authors: SK
authors: Mukherjee
authors: Insect Resistance Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India. raj@icgeb.res.in.
authors: Raj K
authors: RK
authors: Bhatnagar
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D016367:Baculoviridae / Q000235:genetics; D002460:Cell Line; D017930:Genes, Reporter; D049750:Genome, Insect; D049452:Green Fluorescent Proteins / Q000235:genetics; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D034622:RNA Interference; D034741:RNA, Small Interfering; D015203:Reproducibility of Results; D018411:Spodoptera / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins
keywords: Insect RNAi; RNA interference; RNAi screening; Sf21 cells; Spodoptera frugiperda; siRNA screening