title: Lysine Biosynthesis of Thermococcus kodakarensis with the Capacity to Function as an Ornithine Biosynthetic System.
abstract: We recently discovered a biosynthetic system using a novel amino group carrier protein called LysW for lysine biosynthesis via α-aminoadipate (AAA), and revealed that this system is also utilized in the biosynthesis of arginine by Sulfolobus In the present study, we focused on the biosynthesis of lysine and ornithine in the hyperthermophilic archaeon Thermococcus kodakarensis, and showed that their biosynthesis is accomplished by a single set of metabolic enzymes. We also determined the crystal structure of the LysX family protein from T. kodakarensis, which catalyzes the conjugation of LysW with either AAA or glutamate, in a complex with LysW-γ-AAA. This crystal structure is the first example to show how LysX recognizes AAA as a substrate and provides a structural basis for the bifunctionality of the LysX family protein from T. kodakarensis Based on comparisons with other LysX family proteins, we propose a mechanism for substrate recognition and its relationship with molecular evolution among LysX family proteins, which have different substrate specificities.
authors: From the Biotechnology Research Center, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657.
authors: Ayako
authors: A
authors: Yoshida
authors: From the Biotechnology Research Center, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657.
authors: Takeo
authors: T
authors: Tomita
authors: the Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, and.|the Japan Science and Technology Agency, CREST, 7, Gobancho, Chiyoda-ku, Tokyo 102-0076 Japan.
authors: Haruyuki
authors: H
authors: Atomi
authors: From the Biotechnology Research Center, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657.
authors: Tomohisa
authors: T
authors: Kuzuyama
authors: From the Biotechnology Research Center, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, umanis@mail.ecc.u-tokyo.ac.jp.
authors: Makoto
authors: M
authors: Nishiyama
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D008239:Lysine / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D009952:Ornithine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000072417:Protein Domains; D019712:Thermococcus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D002352:Carrier Proteins; D009952:Ornithine; D008239:Lysine
keywords: amino acid; archaea; biosynthesis; carrier protein; crystal structure; evolution; protein complex