title: Improvement of erectile dysfunction by the active pepide from Urechis unicinctus by high temperature/pressure and ultra - wave assisted lysis in Streptozotocin Induced Diabetic Rats.
abstract: BACKGROUND
  We investigate the effect of active peptide from Urechis unicinctus (UU) by high temperature/pressure and ultra-wave assisted lysis on erectile dysfunction in streptozotocin-induced diabetic rats.
  
  
  METHODS
  Forty 12-week-old Sprague-Dawley rats were used in this study. Diabetes was induced by a one-time intraperitoneal injection of streptozotocin (50mg/kg). One week later, the diabetic rats were randomly divided into four groups: normal control, untreated diabetes control, and groups treated with 100 or 500mg/kg/d UU peptide. Rats were fed with UU peptide by intragastric administration for 8 weeks. After 8 weeks, penile hemodynamic function was evaluated in all groups by measuring the intracavernosal pressure after electrostimulating the cavernous nerve. Nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) activities were measured and endothelial nitric oxide synthase (eNOS) and neuronal NOS (nNOS) protein expression. was determined by Western blot.
  
  
  RESULTS
  Maximum intracavernosal pressure in diabetic control rats decreased significantly compared to normal control rats, and was increased significantly compared to untreated diabetic rats after UU peptide supplementation. Treatment with the higher dose of UU peptide significantly increased the NO and cGMP levels compared with the diabetic control group. Decreased activity and expression eNOS and nNOS were found in the diabetic rats compared with the normal control group. Decreased eNOS and nNOS in diabetic rats were improved by UU peptide administration.
  
  
  CONCLUSIONS
  Active peptide from UU ameliorates erectile function in a streptozotocin induced diabetic rat model of erectile dysfunction.
authors: Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Kang Sup
authors: KS
authors: Kim
authors: Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.|Catholic Integrative Medicine Research Institute, The Catholic University of Korea, Seoul, Korea.
authors: Woong Jin
authors: WJ
authors: Bae
authors: Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Su Jin
authors: SJ
authors: Kim
authors: Marine Bioprocess Research Center, Pukyong National University, Busan, Korea.
authors: Kyong-Hwa
authors: KH
authors: Kang
authors: Department of Marine-Bio, Convergence Science and Marine Bioprocess Research Center, Pukyong National University, Busan, Korea.
authors: Se-Kwon
authors: SK
authors: Kim
authors: Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Hyuk Jin
authors: HJ
authors: Cho
authors: Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Sung-Hoo
authors: SH
authors: Hong
authors: Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Ji Youl
authors: JY
authors: Lee
authors: Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea.|Catholic Integrative Medicine Research Institute, The Catholic University of Korea, Seoul, Korea.
authors: Sae Woong
authors: SW
authors: Kim
mesh_terms: D000818:Animals; D000845:Annelida / Q000737:chemistry*; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000150:complications*; D007172:Erectile Dysfunction / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D008297:Male; D010413:Penis / Q000187:drug effects; D010455:Peptides / Q000032:analysis / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D013696:Temperature
chemical_list: D010455:Peptides; D013311:Streptozocin
keywords: Erectile Dysfunction; Streptozocin; Urechis unicinctus [Supplementary Concept]