title: Daidzein increases OPG/RANKL ratio and suppresses IL-6 in MG-63 osteoblast cells.
abstract: Daidzein is a major dietary source of isoflavones found in Leguminosae, and belongs to the family of diphenolic compounds. The estrogenic effects of daidzein to prompt bone formation and prevent bone resorption have been observed in animal models and cultured cells. In our study, we studied the effects of daidzein, raloxifene and E2on expression of the osteoblast-produced bone regulatory factors OPG, RANKL and IL-6 in human osteoblastic MG-63 cells. Results suggest that treatment with daidzein, raloxifene and E2increased the levels of OPG and decreased those of RANKL and IL-6. The effects of daidzein on OPG and RANKL expression are mediated by both ERα and ERβ but those on IL-6 production primarily by ERα. Moreover, daidzein may promote activation of the classic estrogen response element (ERE) pathway through increasing ERα, ERβ and steroid hormone receptor coactivator (SRC)-1 expression. E2was also able to enhance transcription derived from the ERE, while raloxifene has no effect on it. Raloxifene increased ERα protein and gene expression levels but had no effect on ERβ protein and gene expression at 0.1μM. E2was found significantly increased the protein and mRNA levels of SRC-1, while raloxifene has no effect on it compared with control. This ability of daidzein to affect osteoblastic cells makes it a good candidate for the treatment of bone loss in postmenopausal women.
authors: Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin, People's Republic of China; Department of Pharmacology, Logistics College of Chinese People's Armed Police Forces, Tianjin, People's Republic of China.
authors: Jing
authors: J
authors: Sun
authors: Department of Medical Administration, The General Hospital of Chinese People's Armed Police Forces, Beijing, People's Republic of China.
authors: Wei Jia
authors: WJ
authors: Sun
authors: Department of Orthodontics, Tianjin Stomatological Hospital, Tianjin, People's Republic of China.
authors: Zheng Yang
authors: ZY
authors: Li
authors: Department of Pharmacology, Logistics College of Chinese People's Armed Police Forces, Tianjin, People's Republic of China.
authors: Ling
authors: L
authors: Li
authors: Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin, People's Republic of China; Department of Pathogenic Biology and Immunology, Logistics College of Chinese People's Armed Police Forces, Tianjin, People's Republic of China. Electronic address: wangyue6808@126.com.
authors: Yue
authors: Y
authors: Wang
authors: Department of Pathogenic Biology and Immunology, Logistics College of Chinese People's Armed Police Forces, Tianjin, People's Republic of China.
authors: Yu
authors: Y
authors: Zhao
authors: Department of Pathogenic Biology and Immunology, Logistics College of Chinese People's Armed Police Forces, Tianjin, People's Republic of China.
authors: Cong
authors: C
authors: Wang
authors: Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin, People's Republic of China.
authors: Li Ren
authors: LR
authors: Yu
authors: Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin, People's Republic of China; Department of Pharmaceutical Chemistry, Logistics College of Chinese People's Armed Police Forces, Tianjin, People's Republic of China.
authors: Ling Zhi
authors: LZ
authors: Li
authors: Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin, People's Republic of China; Ministry of Training, Logistics College of Chinese People's Armed Police Forces, Tianjin, People's Republic of China. Electronic address: zhang78127@tom.com.
authors: Yong Liang
authors: YL
authors: Zhang
mesh_terms: D002460:Cell Line; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000235:genetics; D047629:Estrogen Receptor beta / Q000235:genetics; D004967:Estrogens / Q000494:pharmacology; D000077267:Fulvestrant; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D007529:Isoflavones / Q000494:pharmacology*; D056920:Nuclear Receptor Coactivator 1 / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism*; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D020849:Raloxifene Hydrochloride / Q000494:pharmacology
chemical_list: C506487:ESR1 protein, human; D004965:Estrogen Antagonists; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; C508600:IL6 protein, human; D015850:Interleukin-6; D007529:Isoflavones; D053244:Osteoprotegerin; D053245:RANK Ligand; D034741:RNA, Small Interfering; C506030:TNFRSF11B protein, human; C505688:TNFSF11 protein, human; D000077267:Fulvestrant; D020849:Raloxifene Hydrochloride; D004958:Estradiol; C004742:daidzein; C538859:NCOA1 protein, human; D056920:Nuclear Receptor Coactivator 1
keywords: Daidzein; ERE; IL-6; OPG; RANKL