title: 3D-engineering of Cellularized Conduits for Peripheral Nerve Regeneration.
abstract: Tissue engineered conduits have great promise for bridging peripheral nerve defects by providing physical guiding and biological cues. A flexible method for integrating support cells into a conduit with desired architectures is wanted. Here, a 3D-printing technology is adopted to prepare a bio-conduit with designer structures for peripheral nerve regeneration. This bio-conduit is consisted of a cryopolymerized gelatin methacryloyl (cryoGelMA) gel cellularized with adipose-derived stem cells (ASCs). By modeling using 3D-printed "lock and key" moulds, the cryoGelMA gel is structured into conduits with different geometries, such as the designed multichannel or bifurcating and the personalized structures. The cryoGelMA conduit is degradable and could be completely degraded in 2-4 months in vivo. The cryoGelMA scaffold supports the attachment, proliferation and survival of the seeded ASCs, and up-regulates the expression of their neurotrophic factors mRNA in vitro. After implanted in a rat model, the bio-conduit is capable of supporting the re-innervation across a 10 mm sciatic nerve gap, with results close to that of the autografts in terms of functional and histological assessments. The study describes an indirect 3D-printing technology for fabricating cellularized designer conduits for peripheral nerve regeneration, and could lead to the development of future nerve bio-conduits for clinical use.
authors: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan province, China.|Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, Sichuan province, China.
authors: Yu
authors: Y
authors: Hu
authors: Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, Sichuan province, China.
authors: Yao
authors: Y
authors: Wu
authors: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan province, China.
authors: Zhiyuan
authors: Z
authors: Gou
authors: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan province, China.
authors: Jie
authors: J
authors: Tao
authors: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan province, China.
authors: Jiumeng
authors: J
authors: Zhang
authors: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan province, China.
authors: Qianqi
authors: Q
authors: Liu
authors: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan province, China.
authors: Tianyi
authors: T
authors: Kang
authors: Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, Sichuan province, China.
authors: Shu
authors: S
authors: Jiang
authors: Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, Sichuan province, China.
authors: Siqing
authors: S
authors: Huang
authors: State key laboratory for manufacturing systems engineering, Xi'an Jiaotong University, Xi'an, 710049,China.
authors: Jiankang
authors: J
authors: He
authors: Department of NanoEngineering, University of California, San Diego, La Jolla, California 92093, USA.
authors: Shaochen
authors: S
authors: Chen
authors: Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084. China.
authors: Yanan
authors: Y
authors: Du
authors: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan province, China.
authors: Maling
authors: M
authors: Gou
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D059985:Cryogels / Q000737:chemistry / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D009416:Nerve Regeneration / Q000187:drug effects*; D059348:Peripheral Nerve Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology*; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology
chemical_list: D059985:Cryogels; D005780:Gelatin