title: Effect of baicalin on hippocampal damage in kainic acid-induced epileptic mice.
abstract: The aim of the present study was to determine the effect of baicalin on the expression of miR-497 and its target B-cell lymphoma-2 (Bcl-2) in the hippocampus of kainic acid (KA)-induced epileptic mice. To establish status epilepticus (SE), 0.1 µg/5 µl KA was injected into the lateral cerebral ventricle in mice, which then received an intraperitoneal injection of baicalin (100 mg/kg) after 1 and 8 h. Hematoxylin and eosin staining was used to observe the pathological changes in morphology and neuronal apoptosis was determined by terminal transferase-mediated dUTP nick end-labeling staining. Western blot analysis was used to detect the expression of Bcl-2 and cleaved caspase-3 proteins in the hippocampus, while reverse transcription-quantitative polymerase chain reaction was used to quantify hippocampal miR-497 expression. The results showed that baicalin significantly attenuated neuronal damage and apoptosis in the hippocampus 72 h after SE. In addition, baicalin decreased SE-induced expression of miR-497 and cleaved caspase-3 protein, while upregulating the expression of Bcl-2 protein. In conclusion, the present results suggest that baicalin possesses potent antiapoptotic properties and attenuates hippocampal injury in mice after SE, which may be associated with the downregulation of miR-497 and cleaved caspase-3 and the upregulation of Bcl-2.
authors: Department of Neurosurgery, Union Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors: Zheng-Jian
authors: ZJ
authors: Liao
authors: Department of Neurosurgery, Union Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors: Ri-Sheng
authors: RS
authors: Liang
authors: Department of Neurosurgery, Union Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors: Song-Sheng
authors: SS
authors: Shi
authors: Department of Neurosurgery, Union Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors: Chun-Hua
authors: CH
authors: Wang
authors: Department of Neurosurgery, Union Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors: Wei-Zhong
authors: WZ
authors: Yang
keywords: B-cell lymphoma-2; apoptosis; baicalin; caspase-3; microRNA-497