title: Regeneration of Cartilage in Human Knee Osteoarthritis with Autologous Adipose Tissue-Derived Stem Cells and Autologous Extracellular Matrix.
abstract: This clinical case series demonstrates that percutaneous injections of autologous adipose tissue-derived stem cells (ADSCs) and homogenized extracellular matrix (ECM) in the form of adipose stromal vascular fraction (SVF), along with hyaluronic acid (HA) and platelet-rich plasma (PRP) activated by calcium chloride, could regenerate cartilage-like tissue in human knee osteoarthritis (OA) patients. Autologous lipoaspirates were obtained from adipose tissue of the abdominal origin. Afterward, the lipoaspirates were minced to homogenize the ECM. These homogenized lipoaspirates were then mixed with collagenase and incubated. The resulting mixture of ADSCs and ECM in the form of SVF was injected, along with HA and PRP activated by calcium chloride, into knees of three Korean patients with OA. The same affected knees were reinjected weekly with additional PRP activated by calcium chloride for 3 weeks. Pretreatment and post-treatment magnetic resonance imaging (MRI) data, functional rating index, range of motion (ROM), and pain score data were then analyzed. All patients' MRI data showed cartilage-like tissue regeneration. Along with MRI evidence, the measured physical therapy outcomes in terms of ROM, subjective pain, and functional status were all improved. This study demonstrates that percutaneous injection of ADSCs with ECM contained in autologous adipose SVF, in conjunction with HA and PRP activated by calcium chloride, is a safe and potentially effective minimally invasive therapy for OA of human knees.
authors: Stems Medical Clinic, Seoul, Republic of Korea.; TEDA-Puhua International Hospital, Tianjin, China.; Life Science Institute, Komplek Permata Senayan, Jalan Tentara Pelajar, Jakarta Selatan, Indonesia.
authors: Jaewoo
authors: J
authors: Pak
authors: Stems Medical Clinic, Seoul, Republic of Korea.; National Leading Research Laboratory, Department of Biological Sciences, Myongji University, Yongin, Republic of Korea.
authors: Jung Hun
authors: JH
authors: Lee
authors: National Leading Research Laboratory, Department of Biological Sciences, Myongji University , Yongin, Republic of Korea.
authors: Kwang Seung
authors: KS
authors: Park
authors: National Leading Research Laboratory, Department of Biological Sciences, Myongji University , Yongin, Republic of Korea.
authors: Byeong Chul
authors: BC
authors: Jeong
authors: National Leading Research Laboratory, Department of Biological Sciences, Myongji University , Yongin, Republic of Korea.
authors: Sang Hee
authors: SH
authors: Lee
keywords: adipose tissue-derived stem cells; extracellular matrix; human cartilage regeneration; osteoarthritis