title: An Integrative Analysis of microRNA and mRNA Profiling in CML Stem Cells.
abstract: Integrative analysis of microRNA (miRNA) and messenger RNA (mRNA) in Chronic Myeloid leukemia (CML) stem cells is an important technique to study the involvement of miRNA and their targets in CML stem cells self-renewal, maintenance, and therapeutic resistance. Here, we describe a simplified integrative analysis using Ingenuity Pathway Analysis software after performing proper RNA extraction, miRNA and mRNA microarray and data analysis.
authors: Department of Biology, Faculty of Arts and Sciences, American University of Beirut, Beirut, Lebanon.
authors: Farah J
authors: FJ
authors: Nassar
authors: Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut, Lebanon.
authors: Rabab
authors: R
authors: El Eit
authors: Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut, Lebanon. rn03@aub.edu.lb.
authors: Rihab
authors: R
authors: Nasr
mesh_terms: D019295:Computational Biology / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D015973:Gene Expression Regulation, Leukemic; D053263:Gene Regulatory Networks; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics*; D017423:Sequence Analysis, RNA; D012984:Software
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: CML stem cells; Ingenuity pathway analysis; RNA extraction; mRNA microarray; miRNA microarray; miRNA-mRNA integration