title: Transcriptome profiling of developmental leaf senescence in sorghum (Sorghum bicolor).
abstract: This piece of the submission is being sent via mail. Leaf senescence is essential for the nutrient economy of crops and is executed by so-called senescence-associated genes (SAGs). Here we explored the monocot C4model crop Sorghum bicolor for a holistic picture of SAG profiles by RNA-seq. Leaf samples were collected at four stages during developmental senescence, and in total, 3396 SAGs were identified, predominantly enriched in GO categories of metabolic processes and catalytic activities. These genes were enriched in 13 KEGG pathways, wherein flavonoid and phenylpropanoid biosynthesis and phenylalanine metabolism were overrepresented. Seven regions on Chromosomes 1, 4, 5 and 7 contained SAG 'hotspots' of duplicated genes or members of cupin superfamily involved in manganese ion binding and nutrient reservoir activity. Forty-eight expression clusters were identified, and the candidate orthologues of the known important senescence transcription factors such as ORE1, EIN3 and WRKY53 showed "SAG" expression patterns, implicating their possible roles in regulating sorghum leaf senescence. Comparison of developmental senescence with salt- and dark- induced senescence allowed for the identification of 507 common SAGs, 1996 developmental specific SAGs as well as 176 potential markers for monitoring senescence in sorghum. Taken together, these data provide valuable resources for comparative genomics analyses of leaf senescence and potential targets for the manipulation of genetic improvement of Sorghum bicolor.
authors: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.|Inner Mongolia Research Centre for Practaculture, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors: Xiao-Yuan
authors: XY
authors: Wu
authors: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.|Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors: Wei-Juan
authors: WJ
authors: Hu
authors: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.|Inner Mongolia Research Centre for Practaculture, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.
authors: Hong
authors: H
authors: Luo
authors: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.|Inner Mongolia Research Centre for Practaculture, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.
authors: Yan
authors: Y
authors: Xia
authors: College of Life Sciences and State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing, 100871, People's Republic of China.
authors: Yi
authors: Y
authors: Zhao
authors: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.|Inner Mongolia Research Centre for Practaculture, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.
authors: Li-Dong
authors: LD
authors: Wang
authors: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.|Inner Mongolia Research Centre for Practaculture, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China.
authors: Li-Min
authors: LM
authors: Zhang
authors: College of Life Sciences and State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing, 100871, People's Republic of China. luojc@pku.edu.cn.
authors: Jing-Chu
authors: JC
authors: Luo
authors: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China. hcjing@ibcas.ac.cn.|Inner Mongolia Research Centre for Practaculture, Chinese Academy of Sciences, Beijing, 100093, People's Republic of China. hcjing@ibcas.ac.cn.
authors: Hai-Chun
authors: HC
authors: Jing
mesh_terms: D018506:Gene Expression Regulation, Plant; D018515:Plant Leaves / Q000235:genetics*; D045868:Sorghum / Q000235:genetics* / Q000254:growth & development*; D059467:Transcriptome / Q000235:genetics*
keywords: Leaf senescence; RNA-seq; Senescence-associated genes; Sorghum bicolor; Transcriptome