title: Amino Acids Hydrolyzed from Animal Carcasses Are a Good Additive for the Production of Bio-organic Fertilizer.
abstract: High-quality bio-organic fertilizers (BIOs) cannot be produced without the addition of some proteins. In this study, compound liquid amino acids (CLAA) from animal carcasses were utilized as additives into matured composts to create novel BIOs containing plant growth-promoting rhizobacteria (PGPR). The results showed that adding CLAA and inoculating bacteria meanwhile resulted in failed solid-state fermentation (SSF) due to the higher H(+) contents. While after pre-compost for 4 days before PGPR inoculation, treatments of matured chicken or pig manure added with 0.2 ml g(-1) of CLAA resulted in a maximum biomass of functional strains. Illumine-MiSeq sequencing and Real-Time PCR results showed that the CLAA addition decreased the bacterial abundance and richness, altered the bacterial community structure and changed the relative abundance of some microbial groups. This study offers a high value-added utilization of waste protein resources for producing economical, high-quality BIO.
authors: Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, National Enginnering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University Nanjing, China.
authors: Hongjun
authors: H
authors: Liu
authors: Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, National Enginnering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University Nanjing, China.
authors: Dandan
authors: D
authors: Chen
authors: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences Beijing, China.
authors: Ruifu
authors: R
authors: Zhang
authors: Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, National Enginnering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University Nanjing, China.
authors: Xinnan
authors: X
authors: Hang
authors: Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, National Enginnering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University Nanjing, China.
authors: Rong
authors: R
authors: Li
authors: Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, National Enginnering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University Nanjing, China.
authors: Qirong
authors: Q
authors: Shen
keywords: Illumine-MiSeq sequencing; bio-organic fertilizer; compound liquid amino acids; microbial community; plant growth-promoting rhizobacteria; solid-state fermentation