title: Octadecylamine-modified poly (glycidylmethacrylate-divinylbenzene) stationary phase for HPLC determination of N-nitrosamines.
abstract: Poly (glycidylmethacrylate-divinylbenzene) (Poly (GMA-DVB)) microspheres were prepared by the two-staged swelling and polymerization method, and modified with octadecylamine (ODA) to obtain ODA-poly (GMA-DVB) stationary phase for HPLC. The new material was characterized by scanning electron microscope, nitrogen adsorption-desorption measurement, Fourier transform infrared spectrum, elemental analysis and thermogravimetric analysis. The results showed that poly (GMA-DVB) microspheres had good monodispersity, porosity and ball shapes. The diameters and specific surface area of the microspheres were about 6µm and 396m(2)g(-1), respectively. ODA-poly (GMA-DVB) stationary phase had good thermal stability. Furthermore, the chromatographic performance of the stationary phase was illustrated by separating n-alkylbenzenes, mono-substituted benzenes and N-nitrosamines. Auxiliary quantum chemistry calculation was also carried out to evaluate the interaction mechanism. According to the evaluation, ODA-poly (GMA-DVB) stationary phase exhibited good hydrophobicity and hydrophobic selectivity, strong stereo-selectivity, polar interaction and π-π interaction. The multi-interaction mechanisms could very likely guarantee its excellent chromatographic performance for the analysis of complex samples. Finally, the column was successfully applied in the determination of N-nitrosamines in pickles sample.
authors: Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
authors: Junwei
authors: J
authors: Liu
authors: Institute of Forensic Science and Technology of Nanjing Public Security Bureau, Nanjing 210001, China.
authors: Yong
authors: Y
authors: Wang
authors: Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
authors: Weixia
authors: W
authors: Li
authors: Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.
authors: Peimin
authors: P
authors: Zhang
authors: Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China; Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China. Electronic address: wnn8511@163.com.
authors: Nani
authors: N
authors: Wang
authors: Zhejiang Institute of Geology and Mineral Resources, Hangzhou 310007, China.
authors: Shuchao
authors: S
authors: Wu
authors: Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China. Electronic address: zhuyan@zju.edu.cn.
authors: Yan
authors: Y
authors: Zhu
mesh_terms: D000588:Amines / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D018553:Cucumis sativus; D005638:Fruit / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D008863:Microspheres; D009602:Nitrosamines / Q000032:analysis* / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000588:Amines; D009602:Nitrosamines; D011109:Polymethacrylic Acids; D011137:Polystyrenes; D011145:Polyvinyls; C516630:poly(glycidyl methacrylate-co-divinylbenzene); C009317:stearylamine
keywords: Interaction mechanism; N-nitrosamines; Octadecylamine; Poly (glycidylmethacrylate-divinylbenzene); Stationary phase