title: Identification of promising plasma immune biomarkers to differentiate active pulmonary tuberculosis.
abstract: Although much research has been done related to biomarker discovery for tuberculosis infection, a set of biomarkers that can discriminate between active and latent TB diseases remains elusive. In the current study we correlate clinical aspects of TB disease with changes in the immune response as determined by biomarkers detected in plasma. Our study measured 18 molecules in human plasma in 17 patients with active disease (APTB), 14 individuals with latent tuberculosis infection (LTBI) and 16 uninfected controls (CTRL). We found that active tuberculosis patients have increased plasma levels of IL-6, IP-10, TNF-α, sCD163 and sCD14. Statistical analysis of these biomarkers indicated that simultaneous measurement of sCD14 and IL-6 was able to diagnose active tuberculosis infection with 83% accuracy. We also demonstrated that TNF-α and sCD163 were correlated with tuberculosis severity. We showed that the simultaneous detection of both plasma sCD14 and IL-6 is a promising diagnostic approach to identify APTB, and further, measurement of TNF-α and sCD163 can identify the most severe cases of tuberculosis.
authors: Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil. Electronic address: fabianazambuzi@usp.br.
authors: Fabiana A
authors: FA
authors: Zambuzi
authors: Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil. Electronic address: priscilla.mariane@hotmail.com.
authors: Priscilla M
authors: PM
authors: Cardoso-Silva
authors: Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil. Electronic address: msespindola@usp.br.
authors: Milena S
authors: MS
authors: Espindola
authors: Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil. Electronic address: luanasisoares@usp.br.
authors: Luana S
authors: LS
authors: Soares
authors: Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil. Electronic address: galvaolima@usp.br.
authors: Leonardo J
authors: LJ
authors: Galvão-Lima
authors: Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil. Electronic address: veronica.brauer@hotmail.com.
authors: Verônica S
authors: VS
authors: Brauer
authors: Laboratorio de Bioinformatica e Analises Moleculares - INGEB/FACOM, Universidade Federal de Uberlandia, Patos de Minas, MG, Brazil. Electronic address: matheus@ingeb.ufu.br.
authors: Matheus S
authors: MS
authors: Gomes
authors: Laboratorio de Bioinformatica e Analises Moleculares - INGEB/FACOM, Universidade Federal de Uberlandia, Patos de Minas, MG, Brazil. Electronic address: laurence@ufu.br.
authors: Laurence R
authors: LR
authors: Amaral
authors: Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA. Electronic address: mschalle@umich.edu.
authors: Matthew
authors: M
authors: Schaller
authors: Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil. Electronic address: bollela@gmail.com.
authors: Valdes R
authors: VR
authors: Bollela
authors: Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil. Electronic address: frantz@usp.br.
authors: Fabiani G
authors: FG
authors: Frantz
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000097:blood*; D008297:Male; D060149:Tetraspanin 30 / Q000097:blood*; D014397:Tuberculosis, Pulmonary / Q000097:blood*
chemical_list: D015415:Biomarkers; C554586:CD63 protein, human; D016207:Cytokines; D018950:Lipopolysaccharide Receptors; D060149:Tetraspanin 30
keywords: Biosignature; Diagnostic; Immune response; Prognostic; Pulmonary tuberculosis