title: CHD7, Oct3/4, Sox2, and Nanog control FoxD3 expression during mouse neural crest-derived stem cell formation.
abstract: Neural crest-derived stem cells (NCSCs) are tissue-specific stem cells derived from multipotent neural crest cells. NCSCs are present in some adult tissues such as dorsal root ganglia, sciatic nerve, and bone marrow. However, little is known about the formation mechanisms of these cells. We have shown that BMP2/Wnt3a signaling and a chromatin remodeler, CHD7, in mice help to maintain the multipotency of neural crest cells and lead to the formation of NCSCs. In the present study, we analyzed a regulatory gene cascade in the formation of mouse NCSCs. The inhibition of FoxD3 expression significantly suppressed the expression of Sox10, which is an indispensable transcription factor for mouse NCSC formation, in the presence of BMP2/Wnt3a. CHD7, Oct3/4, Sox2, and Nanog occupied multiple conserved regions of mouse FoxD3, mE1, mE2, and mE3, in a BMP2/Wnt3a-dependent manner. Furthermore, siRNA of CHD7, Oct3/4, Sox2, and Nanog significantly suppressed FoxD3 expression. The inhibition of histone H3K4 mono- or trimethylation also repressed FoxD3 expression. The present data suggest that CHD7, Oct3/4, Sox2, and Nanog directly induce FoxD3 expression when stimulated by BMP2/Wnt3a signaling, that FoxD3 promotes Sox10 expression, and that histone H3K4 methylation plays important roles in this process of mouse NCSC formation.
authors: Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan.
authors: Kyohei
authors: K
authors: Fujita
authors: Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan.
authors: Ryuhei
authors: R
authors: Ogawa
authors: Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan. itokazuo@bio.sci.osaka-u.ac.jp.
authors: Kazuo
authors: K
authors: Ito
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002478:Cells, Cultured; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D053263:Gene Regulatory Networks; D006657:Histones / Q000378:metabolism; D008745:Methylation; D051379:Mice; D000071317:Nanog Homeobox Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009432:Neural Crest / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D050814:Octamer Transcription Factor-3 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D055758:SOX9 Transcription Factor / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D055757:SOXE Transcription Factors / Q000378:metabolism; D060509:Wnt3A Protein / Q000378:metabolism
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; C507367:Chd7 protein, mouse; D004268:DNA-Binding Proteins; D051858:Forkhead Transcription Factors; C101757:Foxd3 protein, mouse; D006657:Histones; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D012097:Repressor Proteins; D055758:SOX9 Transcription Factor; D055748:SOXB1 Transcription Factors; D055757:SOXE Transcription Factors; C527924:Sox10 protein, mouse; C527783:Sox2 protein, mouse; C527933:Sox9 protein, mouse; D060509:Wnt3A Protein; C555511:Wnt3a protein, mouse
keywords: BMP2/Wnt3a signaling; CHD7; FoxD3; mouse neural crest-derived stem cells; pluripotent stem cell-related genes