title: Surface Engineered Protein Nanoparticles With Hyaluronic Acid Based Multilayers For Targeted Delivery Of Anticancer Agents.
abstract: Layer-by-layer (LbL) technique was employed to modify the surface of doxorubicin (Dox)-loaded bovine serum albumin (BSA) nanoparticles using hyaluronic acid (HA) to enable targeted delivery to overexpressed CD44 receptors in metastatic breast cancer cells. LbL technique offers a versatile approach to modify the surface of colloidal nanoparticles without any covalent modification. Dox-loaded BSA (Dox Ab) nanoparticles optimized for their size, zeta potential, and drug encapsulation efficiency were prepared by modified desolvation technique. The cellular uptake and cytotoxicity of the LbL coated Dox Ab nanoparticles were analyzed in CD44 overexpressing breast cancer cell line MDA-MB-231. Nanoparticles with HA as the final layer (Dox Ab HA) showed maximum cellular uptake in MDA-MB-231 cells owing to the CD44 receptor-mediated endocytosis and hence, exhibited more cytotoxicity as compared to free Dox. Further, luciferase-transfected MDA-MB-231 cells were used to induce tumor in BALB/c female nude mice to enable whole body tumor imaging. The mice were imaged before and after Dox treatment to visualize the tumor growth. The in vivo biodistribution of Dox Ab HA nanoparticles in nude mice showed maximum accumulation in tumor, and importantly, better tumor reduction in comparison with free Dox, thus paving the way for improved drug delivery into tumors.
authors: Department of Materials Engineering, Indian Institute of Science , Bangalore, 560012, India.
authors: Sreeranjini
authors: S
authors: Pulakkat
authors: Molecular Reproduction, Development and Genetics, Indian Institute of Science , Bangalore, 560012, India.|Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University , Manipal, 576104, India.
authors: Sai A
authors: SA
authors: Balaji
authors: Molecular Reproduction, Development and Genetics, Indian Institute of Science , Bangalore, 560012, India.
authors: Annapoorni
authors: A
authors: Rangarajan
authors: Department of Materials Engineering, Indian Institute of Science , Bangalore, 560012, India.|Nanotechnology and Water Sustainability Research Unit, University of South Africa , The Science Campus, Florida Park, 1710 Roodepoort, Johannesburg, South Africa.
authors: Ashok M
authors: AM
authors: Raichur
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents; D045744:Cell Line, Tumor; D004317:Doxorubicin; D016503:Drug Delivery Systems; D005260:Female; D006820:Hyaluronic Acid; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles; D014018:Tissue Distribution
chemical_list: D000970:Antineoplastic Agents; D004317:Doxorubicin; D006820:Hyaluronic Acid
keywords: Cd44; LbL technique; albumin nanoparticles; hyaluronic acid; in vivo targeted delivery