title: PspAG97A: A Halophilic α-Glucoside Hydrolase with Wide Substrate Specificity from Glycoside Hydrolase Family 97.
abstract: A novel α-glucoside hydrolase (named PspAG97A) from glycoside hydrolase family 97 (GH97) was cloned from the deep-sea bacteriumPseudoalteromonassp. K8, which was screened from the sediment of Kongsfjorden. Sequence analysis showed that PspAG97A belonged to GH97, and shared 41% sequence identity with the characterized α-glucosidase BtGH97a. PspAG97A possessed three key catalytically related glutamate residues. Mutation of the glutamate residues indicated that PspAG97A belonged to the inverting subfamily of GH97. PspAG97A showed significant reversibility against changes in salt concentration. It exhibited halophilic ability and improved thermostability in NaCl solution, with maximal activity at 1.0 M NaCl/KCl, and retained more than 80% activity at NaCl concentrations ranging from 0.8 to 2.0 M for over 50 h. Furthermore, PspAG97A hydrolyzed not only α-1,4-glucosidic linkage, but also α-1,6- and α-1,2-glucosidic linkages. Interestingly, PspAG97A possessed high catalytic efficiency for long-chain substrates with α-1,6-linkage. These characteristics are clearly different from other known α-glucoside hydrolases in GH97, implying that PspAG97A is a unique α-glucoside hydrolase of GH97.
authors: School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, P.R. China.
authors: Wei
authors: W
authors: Li
authors: School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, P.R. China.
authors: Han
authors: H
authors: Fan
authors: School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, P.R. China.
authors: Chao
authors: C
authors: He
authors: School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, P.R. China.
authors: Xuecheng
authors: X
authors: Zhang
authors: Department of Chemistry and Biochemistry, Florida International University, Miami, Florida 33199, USA.
authors: Xiaotang
authors: X
authors: Wang
authors: School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, P.R. China.
authors: Jing
authors: J
authors: Yuan
authors: School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, P.R. China.
authors: Zemin
authors: Z
authors: Fang
authors: School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, P.R. China.
authors: Wei
authors: W
authors: Fang
authors: School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, P.R. China.
authors: Yazhong
authors: Y
authors: Xiao
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D031161:Pseudoalteromonas / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology; D016415:Sequence Alignment; D013379:Substrate Specificity; D000520:alpha-Glucosidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006026:Glycoside Hydrolases; D000520:alpha-Glucosidases
keywords: Halophilic; glycoside hydrolase family 97; substrate specificity; α-glucoside hydrolase