title: MicroRNA-184 Promotes Proliferation and Inhibits Apoptosis in HaCaT Cells: An In Vitro Study.
abstract: BACKGROUND This study aimed to investigate the role of miR-184 in the proliferation and apoptosis of keratinocyte (HaCaT cells). MATERIAL AND METHODS HaCaT cells were cultured in a growth medium. The miR-184 was transfected with siRNA, then cell viability and apoptosis were assayed by MTT and flow cytometry, respectively. The colony-forming efficacy of HaCaT cells were detected as well. mRNA expressions of basic fibroblast growth factor (bFGF) and transforming growth factor (TGF)-β1 were measured with RT-PCR. The expressions of apoptosis-related proteins caspase-3 and Bcl-x in HaCaT cells were determined by Western blot. RESULTS After miR-184 was transfected with siRNA, cell viability and colony forming ability decreased significantly, and apoptosis was significantly increased. The expressions of growth factors TGF-β1 and bFGF mRNAs, as well as apoptosis-related proteins Bcl-x, in HaCaT cells declined significantly after miR-184 was transfected with siRNA. In addition, the expression of pro-apoptotic protein caspase-3 increased significantly. CONCLUSIONS Our results suggest distinct roles of miR-184 during the growth, proliferation, and apoptosis of keratinocytes.
authors: Department of Dermatology, First People's Hospital of Nanyang City, Nanyang, Henan, China (mainland).
authors: Xiaodong
authors: X
authors: Bi
authors: Department of Dermatology, First Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China (mainland).
authors: Yu
authors: Y
authors: Cao
authors: Department of Dermatology, First People's Hospital of Nanyang City, Nanyang, Henan, China (mainland).
authors: Rixin
authors: R
authors: Chen
authors: Department of Dermatology, First People's Hospital of Nanyang City, Nanyang, Henan, China (mainland).
authors: Chengyin
authors: C
authors: Liu
authors: Department of Dermatology, First People's Hospital of Nanyang City, Nanyang, Henan, China (mainland).
authors: Jinghong
authors: J
authors: Chen
authors: Department of Dermatology, First People's Hospital of Nanyang City, Nanyang, Henan, China (mainland).
authors: Dongfang
authors: D
authors: Min
mesh_terms: D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; C534424:MIRN184 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D053773:Transforming Growth Factor beta1; D016222:Fibroblast Growth Factor 2; D053148:Caspase 3