title: Optimization of Transfection Conditions for siRNA Screening.
abstract: RNAi screening of mammalian cells is often performed using siRNAs and cationic lipids as transfection reagents. Efficiency of transfection depends on growth characteristics of the cells and the cationic lipid used. With a large selection of cationic lipids available, it can often be difficult to select the optimal lipid and lipid:siRNA (vol:wt) ratio. Here, we describe the process of optimizing siRNA transfection conditions for efficient reverse transfection of mammalian cells using specific positive and negative siRNA controls.
authors: Institute of Molecular Medicine, Phoenix Children's Hospital, Phoenix, AZ, USA.|Department of Child Health, University of Arizona College of Medicine - Phoenix, Phoenix, AZ, USA.
authors: Justin J
authors: JJ
authors: Montoya
authors: Institute of Molecular Medicine, Phoenix Children's Hospital, Phoenix, AZ, USA. dazorsa@email.arizona.edu.|Department of Child Health, University of Arizona College of Medicine - Phoenix, Phoenix, AZ, USA. dazorsa@email.arizona.edu.
authors: David O
authors: DO
authors: Azorsa
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids / Q000737:chemistry; D034741:RNA, Small Interfering; D014162:Transfection / Q000379:methods*
chemical_list: D002412:Cations; D007202:Indicators and Reagents; D008055:Lipids; D034741:RNA, Small Interfering
keywords: Assay; Cationic lipid; RNAi screening; siRNA