title: Lipoic acid functionalized amino acids cationic lipids as gene vectors.
abstract: A series of reducible cationic lipids 4a-4f with different amino acid polar-head groups were prepared. The novel lipid contains a hydrophobic lipoic acid (LA) moiety, which can be reduced under reductive conditions to release of the encapsulated plasmid DNA. The particle size, zeta potential and cellular uptake of lipoplexes formed with DNA, as well as the transfection efficacy (TE) were characterized. The TE of the cationic lipid based on arginine was especially high, and was 2.5times higher than that of a branched polyethylenimine in the presence of 10% serum.
authors: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, Chengdu 610041, PR China.
authors: Rong-Chuan
authors: RC
authors: Su
authors: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, Chengdu 610041, PR China. Electronic address: lqchem@163.com.
authors: Qiang
authors: Q
authors: Liu
authors: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, Chengdu 610041, PR China.
authors: Wen-Jing
authors: WJ
authors: Yi
authors: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, Chengdu 610041, PR China.
authors: Li-Ting
authors: LT
authors: Zheng
authors: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, Chengdu 610041, PR China. Electronic address: zzg63129@163.com.
authors: Zhi-Gang
authors: ZG
authors: Zhao
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D002412:Cations; D005822:Genetic Vectors; D008063:Thioctic Acid / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D002412:Cations; D008063:Thioctic Acid
keywords: Amino acids; Cationic lipid; Lipoic acid; Non-viral vector; Serum