title: Preparation of guar gum scaffold film grafted with ethylenediamine and fish scale collagen, cross-linked with ceftazidime for wound healing application.
abstract: Present study describes the synthesis of carboxymethyl guar gum (CMGG) from the native guar gum (GG) and the prepared CMGG is grafted with ethylenediamine (EDA) to form aminated CMGG. Then, fish scale collagen and aminated CMGG are cross-linked by ceftazidime drug through non- covalent ionic interaction. The resultant cross-linked film is subjected to the analysis of (1)HNMR, ATR-FTIR, TGA, SEM and XRD. The TNBS results revealed that 45% of interaction between EDA and CMGG and 90-95% of Ceftazidime is released from aminated CMGG-Ceftazidime-Collagen (ACCC) film after 96h of incubation at physiological pH. In vitro cell line studies reveal the biocompatibility of the cross-linked film and the antimicrobial studies display the growth inhibition against Staphylococcus aureus and Pseudomonas aeruginosa organisms. Overall, the study indicates that the incorporation of Ceftazidime into collagen and aminated CMGG can improve the functional property of aminated CMGG as well as collagen, leading to its biomedical applications.
authors: Department of Polymer Science & Technology, University of Calcutta, University College of Science & Technology, 92, A.P.C Road, Kolkata 700009, WestBengal, India.
authors: Piyali
authors: P
authors: Jana
authors: Department of Polymer Science & Technology, University of Calcutta, University College of Science & Technology, 92, A.P.C Road, Kolkata 700009, WestBengal, India. Electronic address: tapasbiochem1@gmail.com.
authors: Tapas
authors: T
authors: Mitra
authors: Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu, India.
authors: Thirupathi Kumara Raja
authors: TKR
authors: Selvaraj
authors: Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu, India.
authors: A
authors: A
authors: Gnanamani
authors: Department of Polymer Science & Technology, University of Calcutta, University College of Science & Technology, 92, A.P.C Road, Kolkata 700009, WestBengal, India; Department of Chemical Engineering, Indian Institute of Technology, Roorkee 247667, India. Electronic address: ppk923@yahoo.com.
authors: P P
authors: PP
authors: Kundu
mesh_terms: D000586:Amination; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D002442:Ceftazidime / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D005029:Ethylenediamines / Q000737:chemistry*; D005399:Fishes; D005685:Galactans / Q000737:chemistry*; D006801:Humans; D008351:Mannans / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D053149:Plant Gums / Q000737:chemistry*; D011552:Pseudomonas Infections / Q000188:drug therapy; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D003432:Cross-Linking Reagents; D005029:Ethylenediamines; D005685:Galactans; D008351:Mannans; D053149:Plant Gums; C469704:carboxymethyl guar gum; C031234:ethylenediamine; D003094:Collagen; D002442:Ceftazidime
keywords: Ceftazidime cross-linker; Cyamopsis tetragonoloba seeds; Ethylenediamine; Fish scale collagen; Guar gum