title: Genome-Wide RNAi Screens in C. elegans to Identify Genes Influencing Lifespan and Innate Immunity.
abstract: RNA interference is a rapid, inexpensive, and highly effective tool used to inhibit gene function. In C. elegans, whole genome screens have been used to identify genes involved with numerous traits including aging and innate immunity. RNAi in C. elegans can be carried out via feeding, soaking, or injection. Here we outline protocols used to maintain, grow, and carry out RNAi via feeding in C. elegans and determine whether the inhibited genes are essential for lifespan or innate immunity.
authors: University of Massachusetts Medical School, LRB 770R, 364 Plantation Street, Worcester, MA, 01605, USA.
authors: Amit
authors: A
authors: Sinha
authors: School of Natural Sciences and Psychology, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool, L3 3AF, UK. r.g.rae@ljmu.ac.uk.
authors: Robbie
authors: R
authors: Rae
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D017238:Genes, Helminth; D049751:Genome, Helminth; D023281:Genomics / Q000379:methods*; D007113:Immunity, Innate / Q000235:genetics*; D008136:Longevity / Q000235:genetics*; D034622:RNA Interference
keywords: Ageing; Caenorhabditis elegans; Innate immunity; Lifespan; RNA interference