title: Biochemical and molecular mechanisms underlying the chemopreventive efficacy of rosmarinic acid in a rat colon cancer.
abstract: To shed light on colon cancer chemoprevention, natural phytochemicals attract researchers by virtue of their beneficial biological effects. The chemopreventive potential of rosmarinic acid (RA) was tested by using the colon carcinogen, 1,2-dimethylhydrazine (DMH) by evaluating the Aberrant crypt foci (ACF), tumour incidence, lipid peroxidative byproducts, phase I & II drug metabolizing enzymes, cell proliferative and apoptotic proteins. Rats were divided into six groups and received modified pellet diet. Group 1 served as control rats, group 2 rats received RA (5mg/kg b.w. p.o.), rats in groups 3-6 received DMH (20mg/kg b.w., s.c.) for the first fifteen weeks. In addition to DMH, groups 4-6 received RA at the dose of 5mg/kg b.w. during initiation, post initiation stages and also for the entire study period. DMH treated rats showed an increase in the development of ACF, tumour formation and multiplicity and decrease in lipid peroxidative byproducts. Moreover, it modulates xenobiotic enzymes and reduces the expressions of proapoptotic proteins; increases expressions of anti apoptotic proteins at the end of the study. Supplementation with RA to carcinogen treated rats protected them from the above deleterious effects caused by DMH and thus RA may be used as a potent chemopreventive agent.
authors: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamilnadu, India.
authors: Karthikkumar
authors: K
authors: Venkatachalam
authors: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamilnadu, India.
authors: Sivagami
authors: S
authors: Gunasekaran
authors: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamilnadu, India. Electronic address: nalininam@yahoo.com.
authors: Nalini
authors: N
authors: Namasivayam
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D063646:Carcinogenesis / Q000187:drug effects; D002273:Carcinogens / Q000633:toxicity; D002934:Cinnamates / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology*; D053630:Depsides / Q000494:pharmacology*; D005243:Feces / Q000382:microbiology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects / Q000382:microbiology; D015227:Lipid Peroxidation / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D015262:Xenobiotics / Q000378:metabolism; D000097123:Rosmarinic Acid
chemical_list: D016588:Anticarcinogenic Agents; D002273:Carcinogens; D002934:Cinnamates; D053630:Depsides; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D015262:Xenobiotics; D000097123:Rosmarinic Acid
keywords: ACF; Antioxidants; Apoptosis; Chemoprevention; DMH