title: Protection of a novel epitope-RNA VLP double-effective VLP vaccine for foot-and-mouth disease.
abstract: Foot and mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. Previously, we found that the epitope peptide EP141-160displayed on virus-like particles (VLP) for use as a vaccine showed high immunoreactivity and conferred partially effective protection to animals. In this study, we first combined antisense RNA with VLP as a vaccine against the foot-and-mouth disease virus (FMDV) by using a prokaryotic co-expression system. The antisense RNA against the 3D genes of FMDV was packaged into VLP with EP141-160presented on the surface. ELISA and Western blotting proved that the epitope-RNA VLP eliciting an immune response to FMDV in mice. Furthermore, the potency of the vaccine was tested in mice and guinea pigs. The results indicated that the epitope-RNA VLP vaccine protected 40% of suckling mice and 85% (17/20) of guinea pigs from FMDV. Based on the experimental data, the epitope-RNA VLP vaccine should have value in exploring and developing vaccines against FMDV in the future.
authors: School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China; Zhongshan Hospital, Xiamen University, Xiamen, Fujian 361004, PR China.
authors: Yan-Mei
authors: YM
authors: Dong
authors: Zhongshan Hospital, Xiamen University, Xiamen, Fujian 361004, PR China. Electronic address: jianchunfh2@sina.com.
authors: Jian-Chun
authors: JC
authors: Cai
authors: State Key Laboratory of Veterinary Etiologic Biology, National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, PR China. Electronic address: haotaichen@163.com.
authors: Hao-Tai
authors: HT
authors: Chen
authors: School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China. Electronic address: chenlg@xmu.edu.cn.
authors: Liang
authors: L
authors: Chen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology; D006168:Guinea Pigs; D051379:Mice; D009500:Neutralization Tests; D016372:RNA, Antisense / Q000235:genetics; D012367:RNA, Viral / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D064166:Vaccine Potency; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D016372:RNA, Antisense; D012367:RNA, Viral; D058425:Vaccines, Virus-Like Particle
keywords: Epitope-RNA; Foot-and-mouth disease virus; Potency