title: Chitosan-coated microvesicles: Effect of polysaccharide-phospholipid affinity on decafluorobutane dissolution.
abstract: The stability of perfluorinated microvesicles is mainly determined by the presence of interfacial materials and their ability to hinder the gas component diffusibility into the bloodstream. The goal of this study is to increase the persistence of the gaseous-core by introducing chitosan-coated 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) microvesicles, reducing gas diffusion from microvesicles, and increasing for a long time ultrasonic signals. Our hypothesis was based on the irreversible adhesion of chitosan towards DSPC head groups observed in thin-films models. This affinity enhanced the stabilization of gaseous-core microvesicles, in which the polysaccharide effectively reduced the phospholipid phase transition enthalpy from 383±5.5Jmg(-1) for plain to 150±9.7Jmg(-1) for chitosan-coated microvesicles, providing a more stable structure that diminished the gaseous component lost and provided the persistence of intense (19)F-NMR signals after 48h, twice as long compared to plain samples. As a result, stronger and long-lasting ultrasonic signals were produced by the more stable chitosan-containing microvesicles, thus, presenting great potential to increase the diagnostic and therapeutic applications of perfluorocarbon carries.
authors: Biopol, Chemistry Department, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil; CEB, Pharmacy Department, Federal University of Paraná, 80210-170 Curitiba, PR, Brazil.
authors: Guilherme F
authors: GF
authors: Picheth
authors: Biopol, Chemistry Department, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil.
authors: Cleverton L
authors: CL
authors: Pirich
authors: Biopol, Chemistry Department, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil.
authors: Larissa A
authors: LA
authors: Dos Santos
authors: Medicine Department, Federal University of Paraná, 80060-240 Curitiba, PR, Brazil.
authors: Ana C
authors: AC
authors: Camarozano
authors: Biopol, Chemistry Department, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil.
authors: Maria Rita
authors: MR
authors: Sierakowski
authors: NMR center, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil.
authors: Fernanda M
authors: FM
authors: Ocampos
authors: NMR center, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil.
authors: Andersson
authors: A
authors: Barison
authors: Biopol, Chemistry Department, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil; CEB, Pharmacy Department, Federal University of Paraná, 80210-170 Curitiba, PR, Brazil.
authors: Gabriel A
authors: GA
authors: Kaminski
authors: CEB, Pharmacy Department, Federal University of Paraná, 80210-170 Curitiba, PR, Brazil.
authors: Roberto
authors: R
authors: Pontarolo
authors: Biopol, Chemistry Department, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil. Electronic address: rilton@ufpr.br.
authors: Rilton Alves
authors: RA
authors: de Freitas
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D003287:Contrast Media / Q000737:chemistry*; D005466:Fluorocarbons / Q000737:chemistry*; D045423:Microbubbles; D010713:Phosphatidylcholines / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D012995:Solubility; D014463:Ultrasonography
chemical_list: D003287:Contrast Media; D005466:Fluorocarbons; D010713:Phosphatidylcholines; D010743:Phospholipids; D048271:Chitosan; C010942:1,2-distearoyllecithin; C108042:perfluorobutane
keywords: Chitosan; Decafluorobutane; Microvesicles; Phospholipids; Ultrasound contrast agent