title: Dual-Stage Crosslinking of a Gel-Phase Bioink Improves Cell Viability and Homogeneity for 3D Bioprinting.
abstract: Current bioinks for cell-based 3D bioprinting are not suitable for technology scale-up due to the challenges of cell sedimentation, cell membrane damage, and cell dehydration. A novel bioink hydrogel is presented with dual-stage crosslinking specifically designed to overcome these three major hurdles. This bioink enables the direct patterning of highly viable, multicell type constructs with long-term spatial fidelity.
authors: Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA.
authors: Karen
authors: K
authors: Dubbin
authors: Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA.
authors: Yuki
authors: Y
authors: Hori
authors: Chemical Engineering, Stanford University, Stanford, CA, 94305, USA.
authors: Kazuomori K
authors: KK
authors: Lewis
authors: Materials Science and Engineering, 476 Lomita Mall, Stanford University, Stanford, CA, 94305, USA. Heilshorn@stanford.edu.
authors: Sarah C
authors: SC
authors: Heilshorn
mesh_terms: D000818:Animals; D062028:Bioprinting / Q000379:methods*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D051379:Mice; D041681:NIH 3T3 Cells; D066330:Printing, Three-Dimensional; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: bioink; biomaterials; bioprinting; hydrogels; tissue engineering