title: Lysenin family proteins in earthworm coelomocytes - Comparative approach.
abstract: Sphingomyelin-binding proteins of the lysenin family were originally identified in earthworms belonging to the genus Eisenia comprised of at least two distinct species, E. andrei and E. fetida, until recently considered subspecies or morphotypes of E. foetida (sic). In the present study the presence of lysenin and lysenin-related protein 2 (LRP-2, known also as fetidin) was detected in coelomocytes retrieved from all investigated adult specimens of E. andrei, and E. fetida. They were accompanied by LRP-3 and LRP-1 in some specimens of E. andrei and E. fetida, respectively. Lysenins were not observed in a third composting lumbricid species, Dendrobaena veneta, which served as a convenient negative reference for techniques and procedures used in the study. The pore-forming potential of soluble and cellular fractions of coelomic fluid was studied towards sheep red blood cells and sphingomyelin-rich liposomes. After experimental depletion the potential was restored in parallel with restoration of chloragocyte-derived eleocytes in both E. andrei and E. fetida.
authors: Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland; Laboratory of Proteomics and Mass Spectrometry, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
authors: Bianka
authors: B
authors: Swiderska
authors: Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland; Laboratory of Proteomics and Mass Spectrometry, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
authors: Sylwia
authors: S
authors: Kedracka-Krok
authors: Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors: Tomasz
authors: T
authors: Panz
authors: Cardiff School of Biosciences, Main Building, Cardiff University, Cardiff CF10 3US, Wales, UK.
authors: A John
authors: AJ
authors: Morgan
authors: Department of Malacology, Institute of Zoology, Faculty of Biology and Earth Sciences, Jagiellonian University, Kraków, Poland.
authors: Andrzej
authors: A
authors: Falniowski
authors: Department of Genetics and Evolution, Institute of Zoology, Faculty of Biology and Earth Sciences, Jagiellonian University, Kraków, Poland.
authors: Pawel
authors: P
authors: Grzmil
authors: Department of Evolutionary Immunology, Institute of Zoology, Faculty of Biology and Earth Sciences, Jagiellonian University, Kraków, Poland. Electronic address: barbara.plytycz@uj.edu.pl.
authors: Barbara
authors: B
authors: Plytycz
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006461:Hemolysis; D013058:Mass Spectrometry; D009835:Oligochaeta / Q000276:immunology*; D010586:Phagocytes / Q000276:immunology*; D052899:Pore Forming Cytotoxic Proteins / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D013109:Sphingomyelins / Q000378:metabolism; D014118:Toxins, Biological / Q000235:genetics / Q000378:metabolism*
chemical_list: D052899:Pore Forming Cytotoxic Proteins; D011506:Proteins; D013109:Sphingomyelins; D014118:Toxins, Biological; C103929:fetidin; C107040:lysenin
keywords: COI barcoding; Dendrobaena veneta/Eisenia hortensis; Eisenia andrei; Eisenia fetida; Lysenin-related proteins; Mass spectrometry