title: Replication of Merkel cell polyomavirus induces reorganization of promyelocytic leukemia nuclear bodies.
abstract: Merkel cell polyomavirus (MCPyV) is associated with Merkel cell carcinoma (MCC), a rare but aggressive skin cancer. The virus is highly prevalent: 60-80 % of adults are seropositive; however, cells permissive for MCPyV infection are unknown. Consequently, very little information about the MCPyV life cycle is available. Until recently, MCPyV replication could only be studied using a semi-permissive in vitro replication system (Neumann et al., 2011; Feng et al., 2011, Schowalter et al., 2011). MCPyV replication most likely depends on subnuclear structures such as promyelocytic leukemia protein nuclear bodies (PML-NBs), which are known to play regulatory roles in the infection of many DNA viruses. Here, we investigated PML-NB components as candidate host factors to control MCPyV DNA replication. We showed that PML-NBs change in number and size in cells actively replicating MCPyV proviral DNA. We observed a significant increase in PML-NBs in cells positive for MCPyV viral DNA replication. Interestingly, a significant amount of cells actively replicating MCPyV did not show any Sp100 expression. While PML and Daxx had no effect on MCPyV DNA replication, MCPyV replication was increased in cells depleted for Sp100, strongly suggesting that Sp100 is a negative regulator of MCPyV DNA replication.
authors: Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors: Friederike
authors: F
authors: Neumann
authors: Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors: Manja
authors: M
authors: Czech-Sioli
authors: Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
authors: Thomas
authors: T
authors: Dobner
authors: Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
authors: Adam
authors: A
authors: Grundhoff
authors: Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
authors: Sabrina
authors: S
authors: Schreiner
authors: Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors: Nicole
authors: N
authors: Fischer
mesh_terms: D034961:Antigens, Nuclear / Q000235:genetics / Q000378:metabolism; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism; D015266:Carcinoma, Merkel Cell / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007181:Inclusion Bodies, Viral / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D059965:Merkel cell polyomavirus / Q000235:genetics / Q000502:physiology*; D027601:Polyomavirus Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D000071156:Promyelocytic Leukemia Protein / Q000235:genetics / Q000378:metabolism*; D014412:Tumor Virus Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D014779:Virus Replication
chemical_list: D034961:Antigens, Nuclear; D001324:Autoantigens; D004279:DNA, Viral; D000071156:Promyelocytic Leukemia Protein; C066642:SP100 protein, human