title: [Investigation of Gene Expression Profile of A549 Cells after Overexpression of GPC5  by High Throughput Transcriptome Sequencing].
abstract: BACKGROUND
  Glypican-5 (GPC5) is an important tumor suppressor, while little is known about the impact of GPC5 on proliferation ability and gene expression in lung adenocarcinoma cell lines. Here, we stably overexpressed GPC5 in A549 cells and investigated the impact of cell proliferation ability and gene expression.
  
  
  METHODS
  A549 cells that stably overexpressed GPC5 were constructed by lentivirus. Cell counter kit 8 (CCK8), colony formation, EdU assay were conducted to analyze cell proliferation ability, and transcriptome sequencing was utilized to investigate gene expression profile.
  
  
  RESULTS
  CCK8 assay showed that compared with empty vector, overexpression of GPC5 significantly inhibited cell proliferation rate in A549 cells and the number of colony was also decreased (181±17 vs 278±23). EdU assay also confirmed the percentage of positive staining cells decreased after GPC5 overexpression. Transcriptome sequencing revealed that 2,108 genes were differentially expressed after GPC5 overexpression. Among these differentially expressed genes, 47 genes of the Gene Ontology item "positive regulation of cell proliferation" were downregulated.
  
  
  CONCLUSIONS
  Overexpression of GPC5 inhibited proliferation ability in lung adenocarcinoma A549 cells and genes with the function of "positive regulation of cell proliferation" were downregulated.
authors: Department of Clinical Medicine, Xuzhou Medical University, Xuzhou 221004, China.
authors: Haitian
authors: H
authors: Zhang
authors: Department of Cardiothoracic Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China.
authors: Guoxiang
authors: G
authors: Wang
authors: Department of Oncology, the First People's Hospital of Changzhou, Changzhou 213003, China.
authors: Xin
authors: X
authors: Yang
authors: Department of Thoracic Surgery, Nanjing Medical University Affiliated Cancer Hospital, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Cancer Institute of Jiangsu Province, Nanjing 210009, China.
authors: Mantang
authors: M
authors: Qiu
authors: Department of Thoracic Surgery, Nanjing Medical University Affiliated Cancer Hospital, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Cancer Institute of Jiangsu Province, Nanjing 210009, China.
authors: Lin
authors: L
authors: Xu
mesh_terms: D000072283:A549 Cells; D049109:Cell Proliferation / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D053673:Glypicans / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans
chemical_list: C106228:GPC5 protein, human; D053673:Glypicans