title: Development of an Atlantic salmon heart endothelial cell line (ASHe) that responds to lysophosphatidic acid (LPA).
abstract: As diseases and abnormalities of the heart can interfere with the aquaculture of Atlantic salmon, the heart was investigated as a source of cell lines that could be used to study the cellular basis of these conditions. An Atlantic salmon heart endothelial cell line, ASHe, was developed and characterized for growth properties, endothelial cell characteristics, and responsiveness to lysophosphatidic acid (LPA). AHSe cells stained negative for senescence associated ß-galactosidase and grew well in 10 and 20% FBS/L15 at high cell density, but not in L15 medium supplemented with calf serum. It displayed many endothelial cell-like characteristics including a cobblestone morphology, capillary-like structures formation on Matrigel, and expression of von Willebrand factor and endothelial cell-related tight junction proteins ZO-1, claudin 3, and claudin 5. ASHe cells responded to the cardiovascular modulator, LPA, in two contrasting ways. LPA at 5 and 25 μM inhibited the ability of ASHe cells to heal a wound but stimulated their proliferation, especially as evaluated by colony formation in low-density cultures. The enhancement of proliferation by LPA parallels what has been observed previously in mammalian endothelial cell cultures exposed to LPA, whereas the LPA slowing of ASHe cell migration contrasted with the LPA-enhanced migration of some mammalian cells. Therefore, this cell line is a potentially useful model for future comparative studies on piscine and mammalian cardiovascular cell biology and for studies on diseases of Atlantic salmon in aquaculture.
authors: Department of Biology, University of Waterloo, Waterloo, ON, Canada.
authors: Phuc H
authors: PH
authors: Pham
authors: Department of Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, ON, Canada.
authors: Nguyen T K
authors: NT
authors: Vo
authors: Department of Biology, University of Waterloo, Waterloo, ON, Canada.
authors: Elizabeth J H
authors: EJ
authors: Tan
authors: Department of Fisheries and Aquaculture, Vancouver Island University, Nanaimo, BC, Canada.
authors: Spencer
authors: S
authors: Russell
authors: Elanco Animal Health, PEI, Victoria, Canada.
authors: Ginny
authors: G
authors: Jones
authors: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
authors: John S
authors: JS
authors: Lumsden
authors: Department of Biology, University of Waterloo, Waterloo, ON, Canada. ncbols@uwaterloo.ca.
authors: Niels C
authors: NC
authors: Bols
mesh_terms: D000818:Animals; D002196:Capillaries / Q000187:drug effects / Q000254:growth & development; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line / Q000166:cytology*; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology; D003114:Colony-Forming Units Assay; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004338:Drug Combinations; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D007797:Laminin / Q000494:pharmacology; D008246:Lysophospholipids / Q000494:pharmacology*; D009206:Myocardium / Q000166:cytology*; D011509:Proteoglycans / Q000494:pharmacology; D020317:Salmo salar; D062725:Tight Junction Proteins / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D004338:Drug Combinations; D007797:Laminin; D008246:Lysophospholipids; D011509:Proteoglycans; D062725:Tight Junction Proteins; D014841:von Willebrand Factor; C059485:matrigel; D003094:Collagen; C032881:lysophosphatidic acid
keywords: Atlantic salmon; Capillary formation; Endothelial cell line; Lysophosphatidic acid (LPA); Von Willebrand factor