title: Photoprotective Effects of Cycloheterophyllin against UVA-Induced Damage and Oxidative Stress in Human Dermal Fibroblasts.
abstract: Ultraviolet (UV) radiation, particularly ultraviolet A (UVA), is known to play a major role in photoaging of the human skin. Many studies have demonstrated that UV exposure causes the skin cells to generate reactive oxygen species and activates the mitogen-activated protein kinase (MAPK) pathway. Previous studies have also demonstrated that cycloheterophyllin has an antioxidant effect and can effectively scavenge free radicals. Extending the aforementioned investigations, in this study, human dermal fibroblasts were used to investigate the protective effect of cycloheterophyllin against UV-induced damage. We found that cycloheterophyllin not only significantly increased cell viability, but also attenuated the phosphorylation of MAPK after UVA exposure. Furthermore, cycloheterophyllin could reduce hydrogen peroxide (H2O2) generation and down-regulate H2O2-induced MAPK phosphorylation. In the in vivo studies, the topical application of cycloheterophyllin before UVA irradiation significantly decreased trans-epidermal water loss (TEWL), erythema, and blood flow rate. These results indicate that cycloheterophyllin is a photoprotective agent that inhibits UVA-induced oxidative stress and damage, and could be used in the research on and prevention of skin photoaging.
authors: School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan.|Department of Internal Medicine, Cathay General Hospital, Taipei, Taiwan.
authors: Cheng-Hua
authors: CH
authors: Huang
authors: Department of Chemistry, Fu Jen University, New Taipei City, Taiwan.
authors: Hsin-Ju
authors: HJ
authors: Li
authors: Department of Medicine, Mackay Medical College, New Taipei City, Taiwan.|Department of Dermatology, Mackay Memorial Hospital, Taipei, Taiwan.|Mackay Junior College of Medicine, Nursing, and Management, New Taipei City, Taiwan.
authors: Nan-Lin
authors: NL
authors: Wu
authors: Department of Nutrition and Health Science, Chang Guang University of Science and Technology, Taoyuan, Taiwan.|Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
authors: Chien-Yu
authors: CY
authors: Hsiao
authors: College of Pharmacy, Kaoshiung Medical University, Kaohsiung, Taiwan.
authors: Chun-Nan
authors: CN
authors: Lin
authors: Biomedical Cybernetics Laboratory, Harvard Medical School, Boston, Massachusetts, United States of America.
authors: Hsun-Hsien
authors: HH
authors: Chang
authors: School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan.
authors: Chi-Feng
authors: CF
authors: Hung
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012867:Skin / Q000166:cytology*; D015595:Skin Aging / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D005419:Flavonoids; D011837:Radiation-Protective Agents; C107099:cycloheterophyllin; D020928:Mitogen-Activated Protein Kinases