title: Evaluation of a Silicone Membrane as an Alternative to Human Skin for Determining Skin Permeation Parameters of Chemical Compounds.
abstract: We evaluated the effectiveness of a silicone membrane as an alternative to human skin using the skin permeation parameters of chemical compounds. An in vitro permeation study using 15 model compounds was conducted, and permeation parameters comprising permeability coefficient (P), diffusion parameter (DL(-2)), and partition parameter (KL) were calculated from each permeation profile. Significant correlations were obtained in log P, log DL(-2), and log KL values between the silicone membrane and human skin. DL(-2) values of model compounds, except flurbiprofen, in the silicone membrane were independent of the lipophilicity of the model compounds and were 100-fold higher than those in human skin. For antipyrine and caffeine, which are hydrophilic, KL values in the silicone membrane were 100-fold lower than those in human skin, and P values, calculated as the product of a DL(-2) and KL, were similar. For lipophilic compounds, such as n-butyl paraben and flurbiprofen, KL values for silicone were similar to or 10-fold higher than those in human skin, and P values for silicone were 100-fold higher than those in human skin. Furthermore, for amphiphilic compounds with log Ko/w values from 0.5 to 3.5, KL values in the silicone membrane were 10-fold lower than those in human skin, and P values for silicone were 10-fold higher than those in human skin. The silicone membrane was useful as a human skin alternative in an in vitro skin permeation study. However, depending on the lipophilicity of the model compounds, some parameters may be over- or underestimated.
authors: Skin Care Products Research, Kao Corporation.
authors: Takashi
authors: T
authors: Uchida
authors: Masafumi
authors: M
authors: Yakumaru
authors: Keisuke
authors: K
authors: Nishioka
authors: Yoshihiro
authors: Y
authors: Higashi
authors: Tomohiko
authors: T
authors: Sano
authors: Hiroaki
authors: H
authors: Todo
authors: Kenji
authors: K
authors: Sugibayashi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008954:Models, Biological; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry*; D012828:Silicones / Q000737:chemistry*; D012867:Skin / Q000737:chemistry*; D012869:Skin Absorption; D012995:Solubility
chemical_list: D004364:Pharmaceutical Preparations; D012828:Silicones