title: Characterization of a plasmid carrying cat, ermB and tetS genes in a foodborne Listeria monocytogenes strain and uptake of the plasmid by cariogenic Streptococcus mutans.
abstract: A multi-drug resistant (MDR) Listeria monocytogenes isolate (serotype 1/2c) was recovered from a quick-frozen rice flour product collected from Langfang city in northern China. PCR screening identified the presence of cat, ermB and tetS genes. The plasmid profile of the strain showed the presence of an approximately 22.4-kb plasmid. Curing of this plasmid resulted in the loss of cat, ermB and tetS genes and increased susceptibility to several antibiotics, suggesting the involvement of the plasmid in multiple antibiotic resistances. Moreover, the plasmid was able to be uptaken by human oral pathogen Streptococcus mutans by natural transformation and resulted in the acquiring of multiple resistances in the transconjugants. This study contributes to our knowledge on acquired multi-drug resistance in foodborne pathogenic L.monocytogenes, which will add to a better understanding of effective clinical management of listeriosis.
authors: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, PR China; Department of Veterinary Disease Biology, Faculty Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark.
authors: Lili
authors: L
authors: Li
authors: Department of Veterinary Disease Biology, Faculty Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark.
authors: Rikke Heidemann
authors: RH
authors: Olsen
authors: Research Institute of Food Safety and Nutrition, Jinan University, 510632 Guangzhou, PR China.
authors: Lei
authors: L
authors: Shi
authors: Research Institute of Food Safety and Nutrition, Jinan University, 510632 Guangzhou, PR China.
authors: Lei
authors: L
authors: Ye
authors: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, PR China.
authors: Jianhua
authors: J
authors: He
authors: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, PR China. Electronic address: femenghc@scut.edu.cn.
authors: Hecheng
authors: H
authors: Meng
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D002681:China; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics; D008089:Listeria monocytogenes / Q000187:drug effects / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*; D013295:Streptococcus mutans / Q000187:drug effects / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; C083137:Tet(S) protein, Listeria monocytogenes; D008780:Methyltransferases; D015500:Chloramphenicol O-Acetyltransferase
keywords: Listeria monocytogenes; Multiple resistances; Natural transformation; Plasmid; Streptococcus mutans