title: Enhanced noradrenergic axon regeneration into schwann cell-filled PVDF-TrFE conduits after complete spinal cord transection.
abstract: Schwann cell (SC) transplantation has been utilized for spinal cord repair and demonstrated to be a promising therapeutic strategy. In this study, we investigated the feasibility of combining SC transplantation with novel conduits to bridge the completely transected adult rat spinal cord. This is the first and initial study to evaluate the potential of using a fibrous piezoelectric polyvinylidene fluoride trifluoroethylene (PVDF-TrFE) conduit with SCs for spinal cord repair. PVDF-TrFE has been shown to enhance neurite growth in vitro and peripheral nerve repair in vivo. In this study, SCs adhered and proliferated when seeded onto PVDF-TrFE scaffolds in vitro. SCs and PVDF-TrFE conduits, consisting of random or aligned fibrous inner walls, were transplanted into transected rat spinal cords for 3 weeks to examine early repair. Glial fibrillary acidic protein (GFAP)+astrocyte processes and GFP (green fluorescent protein)-SCs were interdigitated at both rostral and caudal spinal cord/SC transplant interfaces in both types of conduits, indicative of permissivity to axon growth. More noradrenergic/DβH+(dopamine-beta-hydroxylase) brainstem axons regenerated across the transplant when greater numbers of GFAP+astrocyte processes were present. Aligned conduits promoted extension of DβH+axons and GFAP+processes farther into the transplant than random conduits. Sensory CGRP+(calcitonin gene-related peptide) axons were present at the caudal interface. Blood vessels formed throughout the transplant in both conduits. This study demonstrates that PVDF-TrFE conduits harboring SCs are promising for spinal cord repair and deserve further investigation. Biotechnol. Bioeng. 2017;114: 444-456. © 2016 Wiley Periodicals, Inc.
authors: The Miami Project to Cure Paralysis, Lois Pope LIFE Center, University of Miami Miller School of Medicine, P.O. Box 016960, Mail locator R-48, Miami, Florida 33101.
authors: Yee-Shuan
authors: YS
authors: Lee
authors: Department of Material Science and Engineering, New Jersey Institute of Technology, Newark, New Jersey.
authors: Siliang
authors: S
authors: Wu
authors: Department of Biomedical Engineering, New Jersey Institute of Technology, Newark, New Jersey.
authors: Treena Livingston
authors: TL
authors: Arinzeh
authors: The Miami Project to Cure Paralysis, Lois Pope LIFE Center, University of Miami Miller School of Medicine, P.O. Box 016960, Mail locator R-48, Miami, Florida 33101.|Department of Cell Biology, University of Miami Miller School of Medicine, Miami, Florida 33101.|Department of Neurological Surgery, University of Miami Miller School of Medicine, Miami, Florida 33101.
authors: Mary Bartlett
authors: MB
authors: Bunge
mesh_terms: D059331:Adrenergic Neurons / Q000166:cytology / Q000502:physiology*; D000818:Animals; D001369:Axons / Q000502:physiology; D055664:Electrochemical Techniques; D005260:Female; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D051381:Rats; D012583:Schwann Cells / Q000166:cytology* / Q000502:physiology; D013119:Spinal Cord Injuries / Q000628:therapy*; D058630:Spinal Cord Regeneration / Q000502:physiology*; D054457:Tissue Scaffolds / Q000737:chemistry*; D005465:Fluorocarbon Polymers
chemical_list: D006845:Hydrocarbons, Fluorinated; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers; C074585:trifluoroethene
keywords: PVDF-TrFE; Schwann cells; aligned fibers; conduit; electrospinning; spinal cord injury