title: MicroRNA-137 suppresses tongue squamous carcinoma cell proliferation, migration and invasion.
abstract: OBJECTIVE
  Tongue squamous cell carcinoma (TSCC) is the most frequent type of oral malignancy. Increasing evidence has shown that miRNAs play key roles in many biological processes such as cell development, invasion, proliferation, differentiation, metabolism, apoptosis and migration.
  
  
  METHODS
  qRT-PCR analysis was performed to measure miR-137 expression. CCK-8 analysis, cell colony formation, wound-healing analysis and invasion were performed to detect resultant cell functions. The direct target of miR-137 was labelled and measured by luciferase assay and Western blotting.
  
  
  RESULTS
  We demonstrated that expression of miR-137 was downregulated in TSCC tissues compared to matched normal ones. miR-137 expression was downregulated in TSCC lines (SCC4, SCC1, UM1 and Cal27) compared to the immortalized NOK16B cell line and normal oral keratinocytes in culture (NHOK). In addition, we have shown that miR-137 expression was epigenetically regulated in TSCCs. Overexpression of miR-137 suppressed TSCC proliferation and colony formation. Ectopic expression of miR-137 promoted expression of the epithelial biomarker, E-cadherin, and inhibited the mesenchymal biomarker, N-cadherin, as well as vimentin and Snail expression, indicating that miR-137 suppressed TSCC epithelial-mesenchymal transition (EMT). We also showed that ectopic expression of miR-137 inhibited TSCC invasion and migration. In addition, we identified SP1 as a direct target gene of miR-137 in SCC1 cells. SP1 overexpression rescued inhibitory effects exerted by miR-137 on cell proliferation and EMT.
  
  
  CONCLUSIONS
  These results indicate that miR-137 acted as a tumour suppressor in TSCC by targeting SP1.
authors: School of Stomatology, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University, Jinan, 250012, China.|Oral Implantology Center, Stomatology Hospital of Jinan, Jinan, 250001, China.
authors: Lanying
authors: L
authors: Sun
authors: School of Stomatology, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University, Jinan, 250012, China.
authors: Jin
authors: J
authors: Liang
authors: Department of Endodontics, Stomatology Hospital of Jinan, Jinan, 250001, China.
authors: Qibao
authors: Q
authors: Wang
authors: Oral Implantology Center, Stomatology Hospital of Jinan, Jinan, 250001, China.
authors: Zhaoyuan
authors: Z
authors: Li
authors: Department of Endodontics, Stomatology Hospital of Jinan, Jinan, 250001, China.
authors: Yi
authors: Y
authors: Du
authors: School of Stomatology, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University, Jinan, 250012, China. xinxu16@tom.com.
authors: Xin
authors: X
authors: Xu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D016329:Sp1 Transcription Factor / Q000235:genetics; D014059:Tongue / Q000473:pathology*; D014062:Tongue Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C545567:MIRN137 microRNA, human; D035683:MicroRNAs; D016329:Sp1 Transcription Factor