title: A new chitinase-D from a plant growth promoting Serratia marcescens GPS5 for enzymatic conversion of chitin.
abstract: The current study describes heterologous expression and biochemical characterization of single-modular chitinase-D from Serratia marcescens (SmChiD) with unprecedented catalytic properties which include chitobiase and transglycosylation (TG) activities besides hydrolytic activity. Without accessory domains, SmChiD, hydrolyzed insoluble polymeric chitin substrates like colloidal, α- and β-chitin. Activity studies on CHOS with degree of polymerization (DP) 2-6 as substrate revealed that SmChiD hydrolyzed DP2 with a chitobiase activity and showed TG activity on CHOS with DP3-6, producing longer chain CHOS. But, the TG products were further hydrolyzed to shorter chain CHOS with DP1-2 products. SmChiD with its unique catalytic properties, could be a potential enzyme for the production of long chain CHOS and also for the preparation of efficient enzyme cocktails for chitin degradation.
authors: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, India.
authors: Papa Rao
authors: PR
authors: Vaikuntapu
authors: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, India.
authors: Samudrala
authors: S
authors: Rambabu
authors: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, India.
authors: Jogi
authors: J
authors: Madhuprakash
authors: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, India. Electronic address: arpsl@uohyd.ernet.in.
authors: Appa Rao
authors: AR
authors: Podile
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002686:Chitin; D002688:Chitinases / Q000737:chemistry*; D006868:Hydrolysis; D012706:Serratia marcescens / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D002686:Chitin; D002688:Chitinases
keywords: Chitobiase; Chitooligosaccharides; Hydrolysis; SmChiD; Transglycosylation