title: Attenuating properties of Agastache rugosa leaf extract against ultraviolet-B-induced photoaging via up-regulating glutathione and superoxide dismutase in a human keratinocyte cell line.
abstract: Agastache rugosa Kuntze, known as a Korean mint, is an herbal medicine that has been used for the treatment of diverse kinds of symptoms in traditional medicine. This work was undertaken to assess the protective properties of A. rugosa leaves against UV-B-induced photoaging in HaCaT keratinocytes. They were evaluated via analyzing reactive oxygen species (ROS), promatrix metalloproteinase-2 (proMMP-2) and -9 (proMMP-9), total glutathione (GSH), total superoxide dismutase (SOD), cellular viability, flavonoid content and in vitro radical scavenging activity. Total flavonoid content of ARE, a hot water extract of A. rugosa leaves, was 22.8±7.6mg of naringin equivalent/g ARE. ARE exhibited ABTS(+) radical scavenging activity with an SC50 of 836.9μg/mL. ARE attenuated the UV-B-induced ROS generation. It diminished the UV-B-induced elevation of proMMP-2 and -9 at both activity and protein levels. On the contrary, ARE was able to enhance the UV-B-reduced total GSH and total SOD activity levels. ARE, at the used concentrations, was unable to interfere with the cellular viabilities of HaCaT keratinocytes under UV-B irradiation. Taken together, ARE possesses a protective potential against UV-B-induced photoaging in HaCaT keratinocytes, possibly based upon up-regulating antioxidant components, including total GSH and SOD. These findings reasonably suggest the use of A. rugosa leaves as a photoprotective resource in manufacturing functional cosmetics.
authors: Department of Biological Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea.
authors: Yuri
authors: Y
authors: Oh
authors: Shebah Biotech Inc., G-Tech Village, Chuncheon 24398, Republic of Korea.
authors: Hye-Won
authors: HW
authors: Lim
authors: Shebah Biotech Inc., G-Tech Village, Chuncheon 24398, Republic of Korea.
authors: Yu-Hua
authors: YH
authors: Huang
authors: Hankook Cosmo Co., Dongnam-gu, Cheonan 31214, Republic of Korea.
authors: Hee-Souk
authors: HS
authors: Kwon
authors: Department of Biological Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea.
authors: Chang Duck
authors: CD
authors: Jin
authors: Department of Biological Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea. Electronic address: kkim@kangwon.ac.kr.
authors: Kyunghoon
authors: K
authors: Kim
authors: Shebah Biotech Inc., G-Tech Village, Chuncheon 24398, Republic of Korea; Department of Biochemistry, Kangwon National University, Chuncheon 24341, Republic of Korea. Electronic address: cjlim@kangwon.ac.kr.
authors: Chang-Jin
authors: CJ
authors: Lim
mesh_terms: D031327:Agastache / Q000737:chemistry*; D000975:Antioxidants / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D016922:Cellular Senescence / Q000187:drug effects / Q000528:radiation effects; D005419:Flavonoids / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D015854:Up-Regulation / Q000187:drug effects* / Q000528:radiation effects; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D014867:Water; D013482:Superoxide Dismutase; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D005978:Glutathione
keywords: Agastache rugosa; Glutathione; Promatrix metalloproteinase; Reactive oxygen species; Superoxide dismutase; UV-B radiation