title: Treatment of hepatic tumors by thermal versus mechanical effects of pulsed high intensity focused ultrasound in vivo.
abstract: The purpose of this study is to comparatively assess the thermal versus mechanical effects of pulsed high intensity focused ultrasound (HIFU) treatment on hepatic tumors in vivo. Forty-five rabbits with hepatic VX2 tumors were randomly separated into three groups (15 animals per group) before HIFU ablation. The total HIFU energy (in situ) of 1250 J was used for each tumor for three groups. In groups I and II, animals were treated with 1 MHz pulsed ultrasound at 1 Hz pulsed repetition frequency (PRF), 0.5 duty cycle (0.5 s on and 0.5 s off) and10 s duration for one spot sonication. For group II, in addition to HIFU treatment, microbubbles (SonoVue, Bracco, Milan, Italy) were injected via vein before sonication acting as a synergist. In group III, animals were treated with 1 MHz pulsed ultrasound at 10 Hz PRF, 0.1 duty cycle (0.1 s on and 0.9 s off) and 10 s duration for one sonication. The total treatment spots were calculated according to the tumor volume. Tumors were examined with contrast-enhanced computed tomography (CECT) immediately prior to and post HIFU treatment. Histopathologic assessment was performed 3 h after treatment. Our study showed that all animals tolerated the HIFU treatment well. Our data showed that mechanical HIFU could lead to controlled injury in rabbit hepatic tumors with different histological changes in comparison to thermal HIFU with or without microbubbles.
authors: Department of Diagnostic Imaging, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, People's Republic of China.
authors: Song
authors: S
authors: Peng
authors: Ping
authors: P
authors: Zhou
authors: Wei
authors: W
authors: He
authors: Manqiong
authors: M
authors: Liao
authors: Lili
authors: L
authors: Chen
authors: C-M
authors: CM
authors: Ma
mesh_terms: D000818:Animals; D057086:High-Intensity Focused Ultrasound Ablation / Q000379:methods*; D008114:Liver Neoplasms, Experimental / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D045423:Microbubbles; D010743:Phospholipids / Q000378:metabolism*; D011817:Rabbits; D013459:Sulfur Hexafluoride / Q000378:metabolism*; D047368:Tumor Burden
chemical_list: D010743:Phospholipids; C420843:contrast agent BR1; D013459:Sulfur Hexafluoride