title: Immunoglobulin (Ig) D in Labeo rohita is widely expressed and differentially modulated in viral, bacterial and parasitic antigenic challenges.
abstract: Immunoglobulins (Igs) play critical roles in protecting host against diverse pathogenic invasion and diseases. Among all Ig isotypes, IgD is the most recently-evolved and enigmatic molecule detected in all vertebrates species except birds. In South-East Asia, Labeo rohita (rohu) is the leading candidate fish species for freshwater aquaculture, and this article describes about IgD gene expression in rohu following viral, bacterial and parasitic antigenic challenges. The partial cDNA (761bp) of Labeo rohita-IgD (LrIgD) was cloned and submitted in the GenBank with the accession no KT883581. Phylogenetically, LrIgD was closely related to grass carp IgD. Analysis of LrIgD gene expression in juveniles by quantitative real-time PCR (qRT-PCR) assay revealed gradual increase in IgD expression with the advancement of time. In the healthy rohu fingerlings, LrIgD expression occurred predominantly in kidney followed by liver and spleen. In response to rhabdoviral antigenic stimulation, LrIgD expression was significantly enhanced in all tested tissues. In bacterial (Aeromonas hydrophila) infection, transcripts of LrIgD increased more dramatically in liver followed by kidney and gill. In parasitic (Argulus) infection, most significant expression of IgD was noted in the skin, followed by kidney, liver, spleen and gill. These results collectively suggest the key role of IgD in the immune response of rohu during viral, bacterial and parasitic infections.
authors: ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, 751002, India.
authors: Madhubanti
authors: M
authors: Basu
authors: ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, 751002, India.
authors: Saswati S
authors: SS
authors: Lenka
authors: ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, 751002, India.
authors: Mahismita
authors: M
authors: Paichha
authors: ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, 751002, India.
authors: Banikalyan
authors: B
authors: Swain
authors: Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769 008, India.
authors: Bhakti
authors: B
authors: Patel
authors: Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769 008, India.
authors: Rajanya
authors: R
authors: Banerjee
authors: ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, 751002, India.
authors: Pallipuram
authors: P
authors: Jayasankar
authors: Department of Life Science, National Institute of Technology, Rourkela, Odisha, 769 008, India.
authors: Surajit
authors: S
authors: Das
authors: ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, Odisha, 751002, India. Electronic address: msamanta1969@yahoo.com.
authors: Mrinal
authors: M
authors: Samanta
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000276:immunology / Q000662:veterinary*; D003530:Cyprinidae / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology*; D007072:Immunoglobulin D / Q000032:analysis* / Q000235:genetics; D009928:Organ Specificity; D010273:Parasitic Diseases, Animal / Q000276:immunology*; D010802:Phylogeny; D014777:Virus Diseases / Q000276:immunology / Q000662:veterinary*
chemical_list: D007072:Immunoglobulin D
keywords: Aeromonas hydrophila; Argulus; IgD; Immunoglobulins; Labeo rohita; Rhabdovirus vaccine