title: Spautin-1 Ameliorates Acute Pancreatitis via Inhibiting Impaired Autophagy and Alleviating Calcium Overload.
abstract: Acute pancreatitis is characterized by zymogen pre-activation. Severe inflammation caused by zymogen activation can eventually lead to multiple organ dysfunctions, which contributes to the high mortality rate of severe acute pancreatitis. However, there is no specific treatment available for acute pancreatitis therapy. Here, we show that spautin-1, which effectively inhibits autophagy flux, ameliorated the pathogenesis of acute pancreatitis induced by cerulein or L-Arginine. CaMKII phosphorylation due to cytosolic calcium oeverload was revealed in this paper. It was also demonstrated that autophagic protein aggregates degradation blockade accompanying with impaired autophagy correlated positively to intra acinar cells digestive aymogen activation sitimulated by cerulein or L-Arginine. The role of spautin-1 in ameliorating acute pancreatitis was shown here to be associated with impaired autophagy inhibition and Ca2+overload alleviation. We provided a promising therapy for acute pancreatitis here through targeting both impaired autophagy and increased cytosolic calcium.
authors: Youjiang Medical University for Nationalities, Baise 533000, P.R.China.|Western Guangxi Key Laboratory Cultivation Base of Microbial Infection Research.
authors: Juan
authors: J
authors: Xiao
authors: Youjiang Medical University for Nationalities, Baise 533000, P.R.China.
authors: Xueping
authors: X
authors: Feng
authors: Western Guangxi Key Laboratory Cultivation Base of Microbial Infection Research.
authors: Xiao-Ying
authors: XY
authors: Huang
authors: Youjiang Medical University for Nationalities, Baise 533000, P.R.China.|Western Guangxi Key Laboratory Cultivation Base of Microbial Infection Research.
authors: Zhongshi
authors: Z
authors: Huang
authors: Youjiang Medical University for Nationalities, Baise 533000, P.R.China.|Western Guangxi Key Laboratory Cultivation Base of Microbial Infection Research.
authors: Yanqiang
authors: Y
authors: Huang
authors: Youjiang Medical University for Nationalities, Baise 533000, P.R.China.
authors: Chaogan
authors: C
authors: Li
authors: Youjiang Medical University for Nationalities, Baise 533000, P.R.China.
authors: Genliang
authors: G
authors: Li
authors: Youjiang Medical University for Nationalities, Baise 533000, P.R.China.
authors: Song
authors: S
authors: Nong
authors: Department of Pathology, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, P.R.China.
authors: Ruoshi
authors: R
authors: Wu
authors: Department of Pathology, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, P.R.China.
authors: Yongzhi
authors: Y
authors: Huang
authors: Department of Pathology, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, P.R.China.|Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R.China.
authors: Xi-Dai
authors: XD
authors: Long
keywords: Acute pancreatitis; Calcium overload; Cerulein; L-Arginine; impaired autophagy; spautin-1