title: Determination of dopamine hydrochloride by host-guest interaction based on water-soluble pillar[5]arene.
abstract: The supramolecular interaction between the water-soluble pillar[5]arene (WP[5]) as host and dopamine hydrochloride (DH) as guest was studied by spectrofluorometry. The fluorescence intensity of DH gradually decreased with increasing WP[5] concentration, and the possible interaction mechanism between WP[5] and DH was confirmed by1H NMR, 2D NOESY, and molecular modelling. Based on significant DH fluorescence, a highly sensitive and selective method for DH determination was developed for the first time. The fluorescence intensity was measured at 312nm, with excitation at 285nm. The effects of pH, temperature, and reaction time on the fluorescence spectra of the WP[5]-DH complex were investigated. A linear relationship between fluorescence intensity and DH concentration in the range of 0.07-6.2μgmL-1was obtained. The corresponding linear regression equation is ΔF=25.76 C+13.56 (where C denotes the concentration in μgmL-1), with the limit of detection equal to 0.03μgmL-1and the correlation coefficient equal to 0.9996. This method can be used for the determination of dopamine in injection and urine samples. In addition, the WP[5]-DH complex has potential applications in fluorescent sensing and pharmacokinetics studies of DH.
authors: Department of Chemistry, Shanxi Normal University, Linfen 041004, China.
authors: Xue-Dong
authors: XD
authors: Xiao
authors: Department of Chemistry, Shanxi Normal University, Linfen 041004, China.
authors: Lin
authors: L
authors: Shi
authors: Department of Chemistry, Shanxi Normal University, Linfen 041004, China.
authors: Li-Hui
authors: LH
authors: Guo
authors: Department of Chemistry, Shanxi Normal University, Linfen 041004, China. Electronic address: wangjunwen2013@126.com.
authors: Jun-Wen
authors: JW
authors: Wang
authors: Department of Chemistry, Shanxi Normal University, Linfen 041004, China. Electronic address: Xiangzh2000@hotmail.com.
authors: Xiang
authors: X
authors: Zhang
mesh_terms: D002138:Calibration; D047250:Calixarenes; D004298:Dopamine / Q000032:analysis* / Q000737:chemistry* / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D000644:Quaternary Ammonium Compounds; C570642:pillar(5)arene; D047250:Calixarenes; D004298:Dopamine
keywords: Dopamine hydrochloride; Spectrofluorometry; Supramolecular interaction; Water-soluble pillar[5]arene