title: Complete genomic sequence and comparative analysis of four genital and respiratory isolates of bovine herpesvirus subtype 1.2b (BoHV-1.2b), including the prototype virus strain K22.
abstract: Bovine herpesvirus subtype 1.2b (BoHV-1.2b) is associated primarily with bovine infectious pustular vulvovaginitis. We report here the complete genomic sequence of four BoHV-1.2b isolates. The DNA sequence identity of the four genomes is 98.9 %. Differences were primarily in regions containing direct repeats, specifically gene UL36 and the terminal repeat regions immediately flanking gene BICP22. BoHV-1.2b and BoHV-1.1 genomes are similar in size (~135 kb), completely orthologous with respect to regional structure and gene location, and have a 97.5 % DNA sequence homology. The most notable difference is the structure of the DNA replication origin of the two viruses.
authors: Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, 118 McElroy Hall, Oklahoma State University, Stillwater, OK, 74078, USA. jean.doffay@okstate.edu.
authors: Jean M
authors: JM
authors: d'Offay
authors: Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, 118 McElroy Hall, Oklahoma State University, Stillwater, OK, 74078, USA.
authors: R
authors: R
authors: Eberle
authors: Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, 118 McElroy Hall, Oklahoma State University, Stillwater, OK, 74078, USA.
authors: Robert W
authors: RW
authors: Fulton
authors: Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Meanagle, NSW, Australia.
authors: Peter D
authors: PD
authors: Kirkland
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D023061:Gene Order; D014644:Genetic Variation; D005835:Genitalia / Q000821:virology*; D016679:Genome, Viral; D006566:Herpesviridae Infections / Q000662:veterinary / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D018741:Replication Origin; D012137:Respiratory System / Q000821:virology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D026801:Synteny
chemical_list: D004279:DNA, Viral
keywords: BHV-1; BRD; BoHV-1; BoHV-1.1; BoHV-1.2b; Bovine herpesvirus; Bovine respiratory disease; Complete genome sequence; IPB; IPV; Infectious pustular balanoposthitis; Infectious pustular vulvovaginitis; Subtype 1.1; Subtype 1.2b