title: CD25 siRNA induces Treg/Th1 cytokine expression in rat corneal transplantation models.
abstract: Corneal graft rejection is the major reason for transplant failure. CD25 plays an important role in the induction of corneal graft rejection by regulating CD4(+) T cell function. Furthermore, CD25-mediated signaling is closely associated with the expression of Treg cytokines (IL-10, TGF-β) and Th1 cytokines (IFN-γ, IL-1β, and TNF-α). In the current study, corneal transplantation was performed on Wistar rats as donors and Sprague-Dawley rats as recipients. The survival curves indicated that CD25 siRNA treatment significantly prolonged graft survival time (mean survival time [MST], 14.8 ± 0.7 days) as compared with controls (MST, 7.6 ± 0.7 days; n = 12, p < 0.01). HE staining showed that CD25 siRNA alleviated inflammatory cell infiltration. At days 3, 7, 14, and 21, the mRNA and protein expression of CD25 in the CD25 siRNA groups were less than those of the control group, although the most significant decrease of CD25 protein was at day 3. The expression of IL-10 and TGF-β in the CD25 siRNA group increased, while IFN-γ, IL-1β, and TNF-α expression decreased, as well as no significant changes in Foxp3 expression were observed at day 14 post-operation. In conclusion, CD25 siRNA gene therapy played a protective role in corneal graft rejection via up-regulation of Treg cytokine expression and down-regulation of Th1 cytokine expression.
authors: Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Bank, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China.
authors: Qin
authors: Q
authors: Qin
authors: Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Bank, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China.
authors: Dan
authors: D
authors: Luo
authors: Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Bank, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China.
authors: Yunjie
authors: Y
authors: Shi
authors: Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Bank, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China.
authors: Qingqing
authors: Q
authors: Zhao
authors: Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Bank, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China.
authors: Yuan
authors: Y
authors: Chen
authors: Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Bank, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China.
authors: Jing
authors: J
authors: Wu
authors: Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Bank, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China. Electronic address: minzhaomz@outlook.com.
authors: Min
authors: M
authors: Zhao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D016039:Corneal Transplantation; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D015316:Genetic Therapy / Q000379:methods*; D006084:Graft Rejection / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D053645:Interleukin-2 Receptor alpha Subunit / Q000627:therapeutic use*; D012313:RNA / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics; D014184:Transplantation, Homologous
chemical_list: D053645:Interleukin-2 Receptor alpha Subunit; D034741:RNA, Small Interfering; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D012313:RNA
keywords: CD25; CD25 siRNA treatment; Th1 cytokines; Treg cytokines