title: Vertical Transmission of Zika Virus in Aedes aegypti Mosquitoes.
abstract: Previous experimental studies have demonstrated that a number of mosquito-borne flavivirus pathogens are vertically transmitted in their insect vectors, providing a mechanism for these arboviruses to persist during adverse climatic conditions or in the absence of a susceptible vertebrate host. In this study, designed to test whether Zika virus (ZIKV) could be vertically transmitted, female Aedes aegypti and Aedes albopictus were injected with ZIKV, and their F1adult progeny were tested for ZIKV infection. Six of 69 Ae. aegypti pools, comprised of a total of 1,738 F1adults, yielded ZIKV upon culture, giving a minimum filial infection rate of 1:290. In contrast, none of 803 F1Ae. albopictus adults (32 pools) yielded ZIKV. The MFIR for Ae. aegypti was comparable to MFIRs reported for other flaviviruses in mosquitoes, including dengue, Japanese encephalitis, yellow fever, West Nile, and St. Louis encephalitis viruses. The results suggest that vertical transmission may provide a potential mechanism for the virus to survive during adverse conditions.
authors: Department of Pathology, Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors: Saravanan
authors: S
authors: Thangamani
authors: Department of Pathology, Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors: Jing
authors: J
authors: Huang
authors: Department of Pathology, Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors: Charles E
authors: CE
authors: Hart
authors: Department of Pathology, Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors: Hilda
authors: H
authors: Guzman
authors: Department of Pathology, Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, Texas.
authors: Robert B
authors: RB
authors: Tesh
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D005260:Female; D018445:Infectious Disease Transmission, Vertical; D007303:Insect Vectors / Q000821:virology; D000071244:Zika Virus / Q000302:isolation & purification*; D000071243:Zika Virus Infection / Q000635:transmission*