title: Heme oxygenase-1 retards hepatocellular carcinoma progression through the microRNA pathway.
abstract: Heme metabolism system is involved in microRNA (miRNA) biogenesis. The complicated interplay between heme oxygenase-1 (HO-1) and miRNA has been observed in various tissues and diseases, including human malignancy. In the present study, our data showed that stable HO-1 overexpression in hepatocellular carcinoma (HCC) cells downregulated several oncomiRs. The most stably downregulated are miR-30d and miR-107. Iron, one of HO-1 catalytic products, was an important mediator in this regulation. Cell function analysis demonstrated that HO-1 inhibited the proliferation and metastasis of HepG2 cells, whereas miR-30d/miR-107 improved the proliferative and migratory ability of HepG2 cells. The beneficial effect of HO-1 in HCC inhibition could be reversed by upregulating miR-30d and miR-107. Akt and ERK pathways may be involved in the regulation of HO-1/miR-30d/miR-107 in HCC. These data indicate that HO-1 significantly suppresses HCC progression by regulating the miR-30d/miR-107 level, suggesting miR-30d/miR-107 regulation as a new molecular mechanism of HO-1 anticancer effect.
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang, P.R. China.
authors: Chaoxia
authors: C
authors: Zou
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang, P.R. China.
authors: Chendan
authors: C
authors: Zou
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang, P.R. China.
authors: Wanpeng
authors: W
authors: Cheng
authors: Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, P.R. China.
authors: Qiang
authors: Q
authors: Li
authors: Department of Hemopathology, General Hospital of Daqing Oil Field, Daqing, Heilongjiang, P.R. China.
authors: Zhongjing
authors: Z
authors: Han
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang, P.R. China.
authors: Xiaona
authors: X
authors: Wang
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang, P.R. China.
authors: Jianfeng
authors: J
authors: Jin
authors: Institute of Clinical Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, P.R. China.
authors: Jiaqi
authors: J
authors: Zou
authors: Institute of Clinical Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, P.R. China.
authors: Zhiyan
authors: Z
authors: Liu
authors: Institute of Clinical Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, P.R. China.
authors: Zhongqiu
authors: Z
authors: Zhou
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang, P.R. China.
authors: Weiming
authors: W
authors: Zhao
authors: Institute of Clinical Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, P.R. China.
authors: Zhimin
authors: Z
authors: Du
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D051547:Heme Oxygenase-1 / Q000096:biosynthesis* / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction
chemical_list: C549045:MIRN107 microRNA, human; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D051547:Heme Oxygenase-1