title: Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status.
abstract: A cell line representative of human high-grade serous ovarian cancer (HGSOC) should not only resemble its tumour of origin at the molecular level, but also demonstrate functional utility in pre-clinical investigations. Here, we report the integrated proteomic analysis of 26 ovarian cancer cell lines, HGSOC tumours, immortalized ovarian surface epithelial cells and fallopian tube epithelial cells via a single-run mass spectrometric workflow. The in-depth quantification of >10,000 proteins results in three distinct cell line categories: epithelial (group I), clear cell (group II) and mesenchymal (group III). We identify a 67-protein cell line signature, which separates our entire proteomic data set, as well as a confirmatory publicly available CPTAC/TCGA tumour proteome data set, into a predominantly epithelial and mesenchymal HGSOC tumour cluster. This proteomics-based epithelial/mesenchymal stratification of cell lines and human tumours indicates a possible origin of HGSOC either from the fallopian tube or from the ovarian surface epithelium.
authors: Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
authors: F
authors: F
authors: Coscia
authors: Department of Obstetrics and Gynecology, Section of Gynecologic Oncology, University of Chicago, Chicago, Illinois 60637, USA.
authors: K M
authors: KM
authors: Watters
authors: Department of Obstetrics and Gynecology, Section of Gynecologic Oncology, University of Chicago, Chicago, Illinois 60637, USA.
authors: M
authors: M
authors: Curtis
authors: Department of Obstetrics and Gynecology, Section of Gynecologic Oncology, University of Chicago, Chicago, Illinois 60637, USA.
authors: M A
authors: MA
authors: Eckert
authors: Department of Obstetrics and Gynecology, Section of Gynecologic Oncology, University of Chicago, Chicago, Illinois 60637, USA.
authors: C Y
authors: CY
authors: Chiang
authors: Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
authors: S
authors: S
authors: Tyanova
authors: Department of Pathology, University of Chicago Medicine, Chicago, Illinois 60637, USA.
authors: A
authors: A
authors: Montag
authors: Department of Pathology, University of Chicago Medicine, Chicago, Illinois 60637, USA.
authors: R R
authors: RR
authors: Lastra
authors: Department of Obstetrics and Gynecology, Section of Gynecologic Oncology, University of Chicago, Chicago, Illinois 60637, USA.
authors: E
authors: E
authors: Lengyel
authors: Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
authors: M
authors: M
authors: Mann
mesh_terms: D045744:Cell Line, Tumor; D066264:Datasets as Topic; D004847:Epithelial Cells / Q000473:pathology*; D005187:Fallopian Tubes / Q000166:cytology / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D060787:Neoplasm Grading; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D010053:Ovary / Q000166:cytology / Q000473:pathology; D061251:Primary Cell Culture; D040901:Proteomics / Q000379:methods*; D059467:Transcriptome