title: Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers.
abstract: In this study, we developed biodegradable sheath-core-structured drug-eluting nanofibers for sustainable delivery of antibiotics (vancomycin and ceftazidime) and recombinant human bone morphogenetic protein (rhBMP-2) via electrospinning. To prepare the biodegradable sheath-core nanofibers, we first prepared solutions of poly(d,l)-lactide-co-glycolide, vancomycin, and ceftazidime in 1,1,1,3,3,3-hexafluoro-2-propanol and rhBMP-2 in phosphate-buffered solution. The poly(d,l)-lactide-co-glycolide/antibiotics and rhBMP-2 solutions were then fed into two different capillary tubes controlled by two independent pumps for coaxial electrospinning. The electrospun nanofiber morphology was observed under a scanning electron microscope. We further characterized the in vitro antibiotic release from the nanofibers via high-performance liquid chromatography and that of rhBMP-2 via enzyme-linked immunosorbent assay and alkaline phosphatase activity. We showed that the biodegradable coaxially electrospun nanofibers could release high vancomycin/ceftazidime concentrations (well above the minimum inhibition concentration [MIC]90) and rhBMP-2 for >4 weeks. These experimental results demonstrate that novel biodegradable nanofibers can be constructed with various pharmaceuticals and proteins for long-term drug deliveries.
authors: Department of Orthopedic Surgery, Chang Gung Memorial Hospital; Department of Mechanical Engineering.
authors: Yung-Hen
authors: YH
authors: Hsu
authors: Department of Mechanical Engineering.
authors: Chang-Tun
authors: CT
authors: Lin
authors: Department of Orthopedic Surgery, Chang Gung Memorial Hospital; Department of Mechanical Engineering.
authors: Yi-Hsun
authors: YH
authors: Yu
authors: Department of Orthopedic Surgery, Chang Gung Memorial Hospital; Department of Mechanical Engineering.
authors: Ying-Chao
authors: YC
authors: Chou
authors: Department of Orthopedic Surgery, Chang Gung Memorial Hospital; Department of Mechanical Engineering.
authors: Shih-Jung
authors: SJ
authors: Liu
authors: Department of Medical Biotechnology and Laboratory Science, School of Medical Technology, Chang Gung University, Tao-Yuan, Taiwan.
authors: Err-Cheng
authors: EC
authors: Chan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry*; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002442:Ceftazidime / Q000494:pharmacology; D016503:Drug Delivery Systems; D065546:Drug Liberation; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D014640:Vancomycin / Q000494:pharmacology; D014867:Water / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D014640:Vancomycin; D002442:Ceftazidime
keywords: biodegradable sheath-core nanofibers; ceftazidime; coaxial electrospinning; release characteristics; rhBMP-2; vancomycin