title: Mixed lymphocyte reaction induced by multiple alloantigens and the role for IL-10 in proliferation inhibition.
abstract: The frequency of T cells that can respond to alloantigens is unusually high. It remains unclear how T cells would respond when stimulated by multiple major histocompatibility complex (MHC) disparate alloantigens in the same cultures. In this report, we examined potential interactions of T cell clones that were stimulated simultaneously by two sets of complete MHC disparate alloantigens using mixed lymphocyte reaction (MLR). In this assay, we observed that proliferation of B6 lymphocytes (H-2b) stimulated by both BALB/c (H-2d) and C3H (H-2k) allogeneic cells was not increased but rather reduced as compared to B6 cells stimulated with either BALB/c or C3H allogeneic cells. Interestingly, interleukin (IL)-10 expressions at both protein level and mRNA level was significantly increased in cultures stimulated with the two MHC alloantigens, while IL-2, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β1 production did not show any differences. In addition, Foxp3 mRNA expression was comparable amongst all groups. In conclusion, we observed an inhibitory effect in T cell proliferation in response to multiple MHC mismatched alloantigens in MLR, and this effect might be associated with the upregulation of IL-10 expression.
authors: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, the Third Military Medical University, China ; Chongqing Key Laboratory for Proteomics of Diseases, Chongqing, China.
authors: Junyi
authors: J
authors: Zhou
authors: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, the Third Military Medical University, China ; Chongqing Key Laboratory for Proteomics of Diseases, Chongqing, China.
authors: Weifeng
authors: W
authors: He
authors: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, the Third Military Medical University, China ; Chongqing Key Laboratory for Proteomics of Diseases, Chongqing, China.
authors: Gaoxing
authors: G
authors: Luo
authors: State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, the Third Military Medical University, China ; Chongqing Key Laboratory for Proteomics of Diseases, Chongqing, China.
authors: Jun
authors: J
authors: Wu
keywords: Interleukin-10; T-lymphocytes; intraclonal competition; mixed lymphocyte reaction