title: Fluorometric Measurement of Individual Stomata Activity and Transpiration via a "Brush-on", Water-Responsive Polymer.
abstract: Much of atmospheric water originates from transpiration, the process by which plants release H2O from pores, known as stomata, that simultaneously intake CO2 for photosynthesis. Controlling stomatal aperture can regulate the extent of water transport in response to dynamic environmental factors including osmotic stress, temperature, light, and wind. While larger leaf regions are often examined, the extent of water vapor release from individual stomata remains unexplored. Using a "brush-on" sensing material, we can now assess transpiration using a water-responsive, polydiacetylene-based coating on the leaves surfaces. By eliciting a fluorometric signal to passing water vapor, we obtained information regarding the activity of individual stomata. In this demonstration, our results prove that this coating can identify the proportion of active stomata and the extent of transpirational diffusion of water in response to different conditions.
authors: Department of Chemical Engineering, Hanyang University, Seoul 04763, Korea.
authors: Minjeong
authors: M
authors: Seo
authors: Department of Chemical Engineering, Hanyang University, Seoul 04763, Korea.
authors: Dong-Hoon
authors: DH
authors: Park
authors: Institute of Nano Science and Technology, Hanyang University, Seoul 04763, Korea.
authors: Chan Woo
authors: CW
authors: Lee
authors: Department of Chemical Engineering, Hanyang University, Seoul 04763, Korea.|Institute of Nano Science and Technology, Hanyang University, Seoul 04763, Korea.
authors: Justyn
authors: J
authors: Jaworski
authors: Department of Chemical Engineering, Hanyang University, Seoul 04763, Korea.|Institute of Nano Science and Technology, Hanyang University, Seoul 04763, Korea.
authors: Jong-Man
authors: JM
authors: Kim
mesh_terms: D001272:Atmosphere / Q000737:chemistry; D002245:Carbon Dioxide / Q000737:chemistry / Q000378:metabolism; D010788:Photosynthesis; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D054046:Plant Stomata / Q000378:metabolism* / Q000502:physiology; D018526:Plant Transpiration; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry; D053279:Polyynes / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D000078789:Polyacetylene Polymer; D011108:Polymers; D014867:Water; D002245:Carbon Dioxide; D053279:Polyynes; C082361:polydiacetylene