title: Histamine regulates murine primary dendritic cell functions.
abstract: OBJECTIVE
  The modulation of antigen uptake and activation of dendritic cells (DCs) by histamine may function as a regulator of inflammation. Therefore, we sought to determine the impact of histamine on antigen uptake by and activation of murine DCs.
  
  
  METHODS
  DCs from spleen and lung were either identified by flow cytometry or were immunomagnetically enriched. Cells were stimulated with histamine, and the regulation of MHC-II and co-stimulatory molecule expression (CD80, CD86, and ICOS-L) and antigen uptake were quantified by flow cytometry. Individual contributions of the histamine receptor subtypes were determined by using the antagonists mepyramine (histamine H1-receptor: H1R), famotidine (H2R), and JNJ 7777120 (H4R).
  
  
  RESULTS
  Histamine accelerated the uptake of soluble antigen via the H1R, H2R, and H4R in splenic DCs. Co-stimulatory molecule expression was enhanced already by enrichment procedures, thus, the analyses were performed in unseparated cell populations. Histamine enhanced the expression of CD86 and ICOS-L while expression of CD80 was unaffected. Antagonism at H1R, H2R, and H4R and at H1R and H4R reduced the histamine-induced enhanced expression of CD86 and ICOS-L, respectively.
  
  
  CONCLUSIONS
  Histamine contributes to the regulation of the immunological synapse by stimulation of antigen uptake and activation of DCs via H1R, H2R, and H4R.
authors: a Institute of Pharmacology, Hannover Medical School , Hannover , Germany ;
authors: Heiko
authors: H
authors: Schenk
authors: a Institute of Pharmacology, Hannover Medical School , Hannover , Germany ;
authors: Detlef
authors: D
authors: Neumann
authors: a Institute of Pharmacology, Hannover Medical School , Hannover , Germany ;|b Institute of Applied Anatomy, Hannover Medical School , Hannover , Germany.
authors: Christina
authors: C
authors: Kloth
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000276:immunology; D051940:B7-2 Antigen / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D006632:Histamine / Q000494:pharmacology*; D054992:Immunological Synapses / Q000276:immunology*; D060945:Inducible T-Cell Co-Stimulator Ligand / Q000276:immunology; D051379:Mice; D011969:Receptors, Histamine H1 / Q000276:immunology
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; C400230:Icosl protein, mouse; D060945:Inducible T-Cell Co-Stimulator Ligand; D011969:Receptors, Histamine H1; D006632:Histamine
keywords: Histamine; activation; antigen uptake; dendritic cells