title: Age-dependent changes in inflammation and extracellular matrix in bovine oviduct epithelial cells during the post-ovulatory phase.
abstract: The mammalian oviduct is an essential site for sperm storage, the transport of gametes, fertilization, and embryo development-functions that are aided by cytokines secreted from oviduct epithelial cells (OECs). Aging leads to cellular and organ dysfunction, with infertility associated with advanced maternal age. Few studies have investigated age-dependent changes in the oviduct as a possible cause of infertility, so we compared OECs from young (30-50 months) versus aged (more than 120 months) cattle. Next-generation sequencing was first used to identify age-related differences in gene expression. Several proinflammatory-related genes (including IL1B, IL1A, IL17C, IL8, S100A8, S100A9, and TNFA) were activated in OECs from aged (more than 120 months) compare to young (30-50 months) individuals, whereas genes associated with extracellular matrix-related factors (COLs, POSTN, BGN, and LUM) were down-regulation in aged OECs. Indeed, IL1 B and IL8 abundance was higher in aged OECs than in young OECs. Young OECs also tended to proliferate faster, and the revolution frequency of young, ciliated OECs was higher than that of their aged counterparts. In contrast, aged OECs possessed more F-actin, an actin cytoskeleton marker associated with reduced elasticity, and contained high levels of reactive oxygen species, which are mediators of inflammation and senescence. These different functional characteristics of bovine OECs during the post-ovulatory phase support the emerging concept of "inflammaging," that is, age-dependent inflammation. Mol. Reprod. Dev. 83: 815-826, 2016 © 2016 Wiley Periodicals, Inc.
authors: Department of Animal Science, Laboratory of Animal Reproduction, Tokyo University of Agriculture, Atsugi, Kanagawa, Japan.
authors: Hazuki
authors: H
authors: Tanaka
authors: Department of Animal Science, Laboratory of Animal Reproduction, Tokyo University of Agriculture, Atsugi, Kanagawa, Japan.
authors: Ayaka
authors: A
authors: Ohtsu
authors: Department of Animal Science, Laboratory of Animal Reproduction, Tokyo University of Agriculture, Atsugi, Kanagawa, Japan.
authors: Shogo
authors: S
authors: Shiratsuki
authors: NODAI Genome Research Center, Tokyo University of Agriculture, Setagaya, Tokyo, Japan.
authors: Ryoka
authors: R
authors: Kawahara-Miki
authors: Department of Animal Science, Laboratory of Animal Reproduction, Tokyo University of Agriculture, Atsugi, Kanagawa, Japan.
authors: Hisataka
authors: H
authors: Iwata
authors: Department of Animal Science, Laboratory of Animal Reproduction, Tokyo University of Agriculture, Atsugi, Kanagawa, Japan.
authors: Takehito
authors: T
authors: Kuwayama
authors: Department of Animal Science, Laboratory of Animal Reproduction, Tokyo University of Agriculture, Atsugi, Kanagawa, Japan. ks205312@nodai.ac.jp.
authors: Koumei
authors: K
authors: Shirasuna
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002417:Cattle; D016207:Cytokines / Q000096:biosynthesis; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism* / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005260:Female; D005786:Gene Expression Regulation; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D010057:Oviducts / Q000378:metabolism* / Q000473:pathology; D010060:Ovulation
chemical_list: D016207:Cytokines; D016326:Extracellular Matrix Proteins