title: Acetylated α-Tubulin Regulated by N-Acetyl-Seryl-Aspartyl-Lysyl-Proline(Ac-SDKP) Exerts the Anti-fibrotic Effect in Rat Lung Fibrosis Induced by Silica.
abstract: Silicosis is the most serious occupational disease in China. The objective of this study was to screen various proteins related to mechanisms of the pathogenesis of silicosis underlying the anti-fibrotic effect of N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) using proteomic profile analysis. We also aimed to explore a potential mechanism of acetylated α-tubulin (α-Ac-Tub) regulation by Ac-SDKP. Two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) were used to assess the different protein expression profiles between control and silicosis rats treated with or without Ac-SDKP. Twenty-nine proteins were identified to be potentially involved in the progression of silicosis and the anti-fibrotic effect of Ac-SDKP. Our current study finds that 1) the lost expression of Ac-Tub-α may be a new mechanism in rat silicosis; 2) treatment of silicotic rats with N-acetyl-Seryl-Aspartyl-Lysyl-Proline (Ac-SDKP) inhibits myofibroblast differentiation and collagen deposition accompanied by stabilizing the expression of α-Ac-Tub in vivo and in vitro, which is related with deacetylase family member 6 (HDAC6) and α-tubulin acetyl transferase (α-TAT1). Our data suggest that α-Ac-Tub regulation by Ac-SDKP may potentially be a new anti-fibrosis mechanism.
authors: Basic Medical Collage, Hebei Medical University, Shijiazhuang, China.
authors: Wang
authors: W
authors: Xiaojun
authors: Basic Medical Collage, Hebei Medical University, Shijiazhuang, China.
authors: Liu
authors: L
authors: Yan
authors: Medical Research Center, North China University of Science and Technology, Tangshan, China.
authors: Xu
authors: X
authors: Hong
authors: Basic Medical Collage, Hebei Medical University, Shijiazhuang, China.
authors: Zhang
authors: Z
authors: Xianghong
authors: Medical Research Center, North China University of Science and Technology, Tangshan, China.
authors: Li
authors: L
authors: Shifeng
authors: Traditional Chinese Medicine Collage, North China University of Science and Technology, Tangshan, China.
authors: Xu
authors: X
authors: Dingjie
authors: Medical Research Center, North China University of Science and Technology, Tangshan, China.
authors: Gao
authors: G
authors: Xuemin
authors: Medical Research Center, North China University of Science and Technology, Tangshan, China.
authors: Zhang
authors: Z
authors: Lijuan
authors: Medical Research Center, North China University of Science and Technology, Tangshan, China.
authors: Zhang
authors: Z
authors: Bonan
authors: Medical Research Center, North China University of Science and Technology, Tangshan, China.
authors: Wei
authors: W
authors: Zhongqiu
authors: Medical Research Center, North China University of Science and Technology, Tangshan, China.
authors: Wang
authors: W
authors: Ruimin
authors: Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA.
authors: Darrell
authors: D
authors: Brann
authors: Basic Medical Collage, Hebei Medical University, Shijiazhuang, China.
authors: Yang
authors: Y
authors: Fang
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D063990:Gene Ontology; D000073864:Histone Deacetylase 6 / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D065840:Protective Factors; D011499:Protein Processing, Post-Translational; D055550:Protein Stability; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000378:metabolism*; D017208:Rats, Wistar; D012822:Silicon Dioxide; D012829:Silicosis / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*
chemical_list: D000123:Acetyltransferases; D000199:Actins; D000804:Angiotensin II; D000073864:Histone Deacetylase 6; D012822:Silicon Dioxide; D014404:Tubulin; C498862:Acta2 protein, rat; C545556:HDAC6 protein, rat