title: Vanilloid Receptor 1 Agonists, Capsaicin and Resiniferatoxin, Enhance MHC Class I-restricted Viral Antigen Presentation in Virus-infected Dendritic Cells.
abstract: DCs, like the sensory neurons, express vanilloid receptor 1 (VR1). Here we demonstrate that the VR1 agonists, capsaicin (CP) and resiniferatoxin (RTX), enhance antiviral CTL responses by increasing MHC class I-restricted viral antigen presentation in dendritic cells (DCs). Bone marrow-derived DCs (BM-DCs) were infected with a recombinant vaccinia virus (VV) expressing OVA (VV-OVA), and then treated with CP or RTX. Both CP and RTX increased MHC class I-restricted presentation of virus-encoded endogenous OVA in BM-DCs. Oral administration of CP or RTX significantly increased MHC class I-restricted OVA presentation by splenic and lymph node DCs in VV-OVA-infected mice, as assessed by directly measuring OVA peptide SIINFEKL-K(b) complexes on the cell surface and by performing functional assays using OVA-specific CD8 T cells. Accordingly, oral administration of CP or RTX elicited potent OVA-specific CTL activity in VV-OVA-infected mice. The results from this study demonstrate that VR1 agonists enhance anti-viral CTL responses, as well as a neuro-immune connection in anti-viral immune responses.
authors: College of Pharmacy, Chungbuk National University, Cheongju 28644, Korea.
authors: Young-Hee
authors: YH
authors: Lee
authors: College of Pharmacy, Chungbuk National University, Cheongju 28644, Korea.
authors: Sun-A
authors: SA
authors: Im
authors: College of Pharmacy, Chungbuk National University, Cheongju 28644, Korea.
authors: Ji-Wan
authors: JW
authors: Kim
authors: College of Pharmacy, Chungbuk National University, Cheongju 28644, Korea.
authors: Chong-Kil
authors: CK
authors: Lee
keywords: Antigen presentation; Dendritic cell; MHC class I; VR1 agonist