title: Placental bed apoptosis is increased in pregnant women with pre-eclampsia versus normotensive pregnant women.
abstract: We hypothesised that apoptosis in the placenta is increased in pregnant women whose pregnancies were complicated by pre-eclampsia as compared to normal pregnant women. Biopsy samples were obtained by punch biopsy from placental beds in 15 pre-eclamptic and 15 normotensive pregnant women during cesarean section. Apoptosis in syncytiotrophoblasts, syncytial cluster, extravillous cytotrophoblast, and decidual and stromal cells were evaluated by caspase-3, bax and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labelling (TUNEL) immunohistochemical methods. A significant involvement was observed via caspase-3 and TUNEL methods in the syncytiotrophoblasts, syncytial cluster and extravillous cytotrophoblast cells of the pre-eclamptic group versus normotensive group (p < 0.001). Caspase-3 method found significantly increased involvement in the pre-eclamptic group versus normotensive group (p < 0.001). Although bax method found significantly increased involvement in syncytiotrophoblasts in the pre-eclamptic group versus normotensive group (p < 0.001), no significant difference was found between the groups in terms of involvement of other cell groups (p > 0.05). Apoptosis in the placental bed is increased in pre-eclamptic woman.
authors: a Department of Obstetrics and Gynecology , M.D. Van Obstetrics and Pediatrics Hospital , Van , Turkey.
authors: Mürüde
authors: M
authors: Dagdelen
authors: b Department of Obstetrics and Gynecology , M.D. Manisa Merkezefendi Hospital , Manisa , Turkey.
authors: Muzaffer
authors: M
authors: Temur
authors: c Department of Obstetrics and Gynecology , M.D. Manisa Merkezefendi Hospital and Manisa Akhisar State Hospital , Manisa , Turkey.
authors: Özgür
authors: Ö
authors: Yılmaz
authors: d Department of Obstetrics and Gynecology , M.D. Kocaeli Anadolu Health Center , Kocaeli , Turkey.
authors: Tamer
authors: T
authors: Altındag
authors: e Department of Obstetrics and Gynecology , M.D. Dokuzeylul Uneversity Medical School , İzmir , Turkey.
authors: Turan
authors: T
authors: Uslu
authors: f Department of Obstetrics and Gynecology , M.D. Aydin Private Ege Liva Hospital , Aydin , Turkey.
authors: Pelin Özün
authors: PÖ
authors: Özbay
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000502:physiology*; D001794:Blood Pressure; D016022:Case-Control Studies; D053148:Caspase 3 / Q000032:analysis; D002585:Cesarean Section; D003656:Decidua / Q000502:physiology; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D010920:Placenta / Q000166:cytology* / Q000473:pathology / Q000503:physiopathology; D011225:Pre-Eclampsia / Q000473:pathology / Q000503:physiopathology*; D011247:Pregnancy; D017154:Stromal Cells / Q000502:physiology; D014327:Trophoblasts / Q000502:physiology
chemical_list: D053148:Caspase 3
keywords: Apoptosis; TUNEL method; caspase-3; placental bed; pre-eclampsia