title: Monitoring of anatabine release by methyl jasmonate elicited BY-2 cells using surface-enhanced Raman scattering.
abstract: A new application of surface-enhanced Raman scattering (SERS) in the field of plant material analysis is proposed in this study. The aim was to monitor the release of anatabine by methyl jasmonate (MeJa) elicited Bright Yellow-2 (BY-2) cells. Gold nanoparticles (AuNps) were used as SERS substrate. The first step was to study the SERS activity of anatabine in a complex matrix comprising the culture medium and BY-2 cells. The second step was the calibration. This one was successfully performed directly in the culture medium in order to take into account the matrix effect, by spiking the medium with different concentrations of anatabine, leading to solutions ranging from 250 to 5000µgL(-1). A univariate analysis was performed, the intensity of a band situated at 1028cm(-1), related to anatabine, was plotted against the anatabine concentration. A linear relationship was observed with a R(2) of 0.9951. During the monitoring study, after the MeJa elicitation, samples were collected from the culture medium containing BY-2 cells at 0, 24h, 48h, 72h and 96h and were analysed using SERS. Finally, the amount of anatabine released in the culture medium was determined using the response function, reaching a plateau after 72h of 82µg of anatabine released/g of fresh weight (FW) MeJa elicited BY-2 cells.
authors: University of Liege (ULg), CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, Quartier Hôpital, Avenue Hippocrate 15, B36, B-4000 Liege, Belgium. Electronic address: cdebleye@ulg.ac.be.
authors: C
authors: C
authors: De Bleye
authors: University of Liege (ULg), CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, Quartier Hôpital, Avenue Hippocrate 15, B36, B-4000 Liege, Belgium.
authors: E
authors: E
authors: Dumont
authors: University of Liege (ULg), CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, Quartier Hôpital, Avenue Hippocrate 15, B36, B-4000 Liege, Belgium.
authors: A
authors: A
authors: Dispas
authors: University of Liege (ULg), CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, Quartier Hôpital, Avenue Hippocrate 15, B36, B-4000 Liege, Belgium.
authors: C
authors: C
authors: Hubert
authors: University of Liege (ULg), CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, Quartier Hôpital, Avenue Hippocrate 15, B36, B-4000 Liege, Belgium.
authors: P-Y
authors: PY
authors: Sacré
authors: University of Liege (ULg), CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, Quartier Hôpital, Avenue Hippocrate 15, B36, B-4000 Liege, Belgium.
authors: L
authors: L
authors: Netchacovitch
authors: University of Liege (ULg), CEDEVIT (ASBL), Plant and Biology Institute, Plant Molecular Biology and Biotechnology Unit, Sart-Tilman, Quartier Vallée 1, Chemin de la Vallée 4, B22, B-4000 Liege, Belgium.
authors: B
authors: B
authors: De Muyt
authors: University of Liege (ULg), CEDEVIT (ASBL), Plant and Biology Institute, Plant Molecular Biology and Biotechnology Unit, Sart-Tilman, Quartier Vallée 1, Chemin de la Vallée 4, B22, B-4000 Liege, Belgium.
authors: C
authors: C
authors: Kevers
authors: University of Liege (ULg), CEDEVIT (ASBL), Plant and Biology Institute, Plant Molecular Biology and Biotechnology Unit, Sart-Tilman, Quartier Vallée 1, Chemin de la Vallée 4, B22, B-4000 Liege, Belgium.
authors: J
authors: J
authors: Dommes
authors: University of Liege (ULg), CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, Quartier Hôpital, Avenue Hippocrate 15, B36, B-4000 Liege, Belgium.
authors: Ph
authors: P
authors: Hubert
authors: University of Liege (ULg), CIRM, Department of Pharmacy, Laboratory of Analytical Chemistry, CHU, Quartier Hôpital, Avenue Hippocrate 15, B36, B-4000 Liege, Belgium.
authors: E
authors: E
authors: Ziemons
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002853:Chromatography, Liquid; D003470:Culture Media / Q000032:analysis; D003517:Cyclopentanes / Q000494:pharmacology*; D006046:Gold / Q000737:chemistry; D013058:Mass Spectrometry; D053768:Metal Nanoparticles / Q000737:chemistry; D054883:Oxylipins / Q000494:pharmacology*; D011725:Pyridines / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D014026:Nicotiana / Q000166:cytology*
chemical_list: D000085:Acetates; D000470:Alkaloids; D003470:Culture Media; D003517:Cyclopentanes; D054883:Oxylipins; D011725:Pyridines; C012737:anatabine; D006046:Gold; C072239:methyl jasmonate
keywords: BY-2 cells; Calibration in the matrix; Gold nanoparticles; Monitoring of anatabine release; Surface-enhanced Raman scattering