title: Role of LncRNA-activated by transforming growth factor beta in the progression of hepatitis C virus-related liver fibrosis.
abstract: Long non-coding RNA (LncRNA)-activated by transforming growth factor-beta (LncRNA-ATB) is a key regulator of transforming growth factor-beta (TGF-β) signaling pathway, and is positively correlated with the development of liver cirrhosis and vascular invasion of hepatocellular carcinoma (HCC). However, the role of LncRNA-ATB in hepatitis C virus (HCV)-related liver fibrosis remains largely unknown. In the present study, we confirmed a high expression level of LncRNA-ATB in the liver tissues and plasma samples of patients with HCV-related hepatic fibrosis, and the plasma level of LncRNA-ATB was significantly correlated with liver fibrosis stages. Furthermore, increased expression level of LncRNA-ATB was also present in activated hepatic stellate cells (HSCs), and knockdown of LncRNA-ATB inhibited the expression of alpha-smooth muscle actin (α-SMA) and alpha-1 type I collagen (Col1A1). LncRNA-ATB was found to share the common miRNA responsive element of miR-425-5p with TGF-β type II receptor (TGF-βRII) and SMAD2. Ectopic expression of LncRNA-ATB in HSCs could upregulate the protein expression of TGF-βRII and SMAD2 by inhibiting the endogenous miR-425-5p. Moreover, overexpression of miR-425-5p could partly abrogate the expression of TGF-βRII and SMAD2 induced by LncRNA-ATB. Hence, we conclude that LncRNA-ATB promotes HCV-induced liver fibrogenesis by activating HSCs and increasing collagen I production through competitively binding to miR-425-5p. LncRNA-ATB may be a novel diagnostic biomarker and a potential therapeutic target for HCV-related hepatic fibrosis.
authors: Department of Traditional and Western Medical Hepatology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
authors: Na
authors: N
authors: Fu
authors: Department of Traditional and Western Medical Hepatology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
authors: Xuemin
authors: X
authors: Niu
authors: Department of Traditional and Western Medical Hepatology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
authors: Yang
authors: Y
authors: Wang
authors: Department of Traditional and Western Medical Hepatology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
authors: Huijuan
authors: H
authors: Du
authors: Department of Traditional and Western Medical Hepatology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
authors: Baoyu
authors: B
authors: Wang
authors: Department of Traditional and Western Medical Hepatology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
authors: Jinghua
authors: J
authors: Du
authors: Department of Experimental Center, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
authors: Ya
authors: Y
authors: Li
authors: Department of Traditional and Western Medical Hepatology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
authors: Rongqi
authors: R
authors: Wang
authors: Department of Traditional and Western Medical Hepatology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
authors: Yuguo
authors: Y
authors: Zhang
authors: Department of Traditional and Western Medical Hepatology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
authors: Suxian
authors: S
authors: Zhao
authors: The Liver Disease Diagnosis and Treatment Center of PLA, Bethune International Peace Hospital, Shijiazhuang, Hebei 050051, China.
authors: Dianxing
authors: D
authors: Sun
authors: Storr Liver Centre, Westmead Millennium Institute for Medical Research, the University of Sydney at Westmead Hospital, Westmead, NSW 2145, Australia.
authors: Liang
authors: L
authors: Qiao
authors: Department of Traditional and Western Medical Hepatology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
authors: Yuemin
authors: Y
authors: Nan
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D005260:Female; D057809:HEK293 Cells; D016174:Hepacivirus / Q000472:pathogenicity*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008103:Liver Cirrhosis / Q000378:metabolism* / Q000473:pathology* / Q000821:virology; D008297:Male; D035683:MicroRNAs / Q000235:genetics; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; D062085:RNA, Long Noncoding; C000619854:long non-coding RNA ATB, human