title: Evaluation of 5-HT7 Receptor Trafficking on In Vivo and In Vitro Model of Lipopolysaccharide (LPS)-Induced Inflammatory Cell Injury in Rats and LPS-Treated A549 Cells.
abstract: This study aimed to investigate the effects of the 5-HT7 receptor agonist (LP44) and antagonist (SB269970) on LPS-induced in vivo tissue damage and cell culture by molecular methods. This study was conducted in two steps. For in vivo studies, 24 female rats were divided into four groups. Group I: healthy; II (2nd h): LPS 5 mg/kg administered intraperitoneally (i.p.); III (4th h): LPS 5 mg/kg administered i.p.; IV (8th h): LPS 5 mg/kg administered i.p. For in vitro studies, we used the A549 cell line. Groups: I control (healthy) (2-4 h); II LPS: 1 µg/ml E. Coli O55:B5 strain (2-4 h); III agonist (LP44) 10-9M (2-4 h); IV antagonist (SB269970) 10-9M (2-4 h); V LPS+agonist 10-9M (LP44 1 µg/ml) (2-4 h); VI LPS+antagonist 10-9M (2-4 h). In molecular analyses, we determined increased TNF-α, IL-1β, NF-κB, and 5-HT7 mRNA expressions in rat lung tissues and increased TNF-α, iNOS, and 5-HT7 mRNA expressions in the A549 cell line. In in vitro parameters, LP44 agonist administration-related decrease was observed. Our study showed that lung 5-HT7 receptor expression is increased in LPS-induced endotoxemia. All this data suggest that 5-HT7 receptor overexpression is an important protective mechanism during LPS-induced sepsis-related cell damage.
authors: Department of Pharmacology, Faculty of Medicine, Ataturk University, Campus, 25240, Erzurum, Turkey.
authors: Gulsen
authors: G
authors: Ayaz
authors: Department of Pharmacology, Faculty of Medicine, Ataturk University, Campus, 25240, Erzurum, Turkey.
authors: Zekai
authors: Z
authors: Halici
authors: Department of Pharmacology, Faculty of Medicine, Ataturk University, Campus, 25240, Erzurum, Turkey. dramecit@gmail.com.
authors: Abdulmecit
authors: A
authors: Albayrak
authors: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey.
authors: Emre
authors: E
authors: Karakus
authors: Department of Pharmacology, Faculty of Medicine, Ataturk University, Campus, 25240, Erzurum, Turkey.
authors: Elif
authors: E
authors: Cadirci
mesh_terms: D000072283:A549 Cells; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D055370:Lung Injury; D008297:Male; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D011985:Receptors, Serotonin / Q000378:metabolism*
chemical_list: D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D011985:Receptors, Serotonin; C104425:serotonin 7 receptor
keywords: 5-HT7; LP44; LPS; SB269970; Sepsis