title: Characterization of Growth and Reproduction Performance, Transgene Integration, Expression, and Transmission Patterns in Transgenic Pigs Produced by piggyBac Transposition-Mediated Gene Transfer.
abstract: Previously we successfully produced a group of EGFP-expressing founder transgenic pigs by a newly developed efficient and simple pig transgenesis method based on cytoplasmic injection of piggyBac plasmids. In this study, we investigated the growth and reproduction performance and characterized the transgene insertion, transmission, and expression patterns in transgenic pigs generated by piggyBac transposition. Results showed that transgene has no injurious effect on the growth and reproduction of transgenic pigs. Multiple copies of monogenic EGFP transgene were inserted at noncoding sequences of host genome, and passed from founder transgenic pigs to their transgenic offspring in segregation or linkage manner. The EGFP transgene was ubiquitously expressed in transgenic pigs, and its expression intensity was associated with transgene copy number but not related to its promoter DNA methylation level. To the best of our knowledge, this is first study that fully described the growth and reproduction performance, transgene insertion, expression, and transmission profiles in transgenic pigs produced by piggyBac system. It not only demonstrates that piggyBac transposition-mediated gene transfer is an effective and favorable approach for pig transgenesis, but also provides scientific information for understanding the transgene insertion, expression and transmission patterns in transgenic animals produced by piggyBac transposition.
authors: a National Engineering Research Center for Breeding Swine Industry, College of Animal Science , South China Agricultural University , Guangzhou , China.|b Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science , South China Agricultural University , Guangzhou , China.
authors: Fang
authors: F
authors: Zeng
authors: a National Engineering Research Center for Breeding Swine Industry, College of Animal Science , South China Agricultural University , Guangzhou , China.|b Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science , South China Agricultural University , Guangzhou , China.
authors: Zicong
authors: Z
authors: Li
authors: c Institute of Animal Science , Guangdong Academy of Agricultural Sciences , Guangzhou , China.
authors: Gengyuan
authors: G
authors: Cai
authors: a National Engineering Research Center for Breeding Swine Industry, College of Animal Science , South China Agricultural University , Guangzhou , China.|b Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science , South China Agricultural University , Guangzhou , China.
authors: Wenchao
authors: W
authors: Gao
authors: a National Engineering Research Center for Breeding Swine Industry, College of Animal Science , South China Agricultural University , Guangzhou , China.|b Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science , South China Agricultural University , Guangzhou , China.
authors: Gelong
authors: G
authors: Jiang
authors: a National Engineering Research Center for Breeding Swine Industry, College of Animal Science , South China Agricultural University , Guangzhou , China.|b Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science , South China Agricultural University , Guangzhou , China.
authors: Dewu
authors: D
authors: Liu
authors: d Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, John A. Burns School of Medicine , University of Hawaii at Manoa , Honolulu , Hawaii , USA.
authors: Johann
authors: J
authors: Urschitz
authors: d Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, John A. Burns School of Medicine , University of Hawaii at Manoa , Honolulu , Hawaii , USA.|e Manoa BioSciences , Honolulu , Hawaii , USA.
authors: Stefan
authors: S
authors: Moisyadi
authors: a National Engineering Research Center for Breeding Swine Industry, College of Animal Science , South China Agricultural University , Guangzhou , China.|b Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science , South China Agricultural University , Guangzhou , China.
authors: Zhenfang
authors: Z
authors: Wu
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D019175:DNA Methylation / Q000235:genetics; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D009929:Organ Size / Q000235:genetics; D013552:Swine / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D019076:Transgenes / Q000235:genetics*
keywords: Copy number; methylation; piggyBac; transgenic pigs; transposition