title: Antimutagenic and antioxidant properties of the aqueous extracts of organic and conventional grapevine Vitis labrusca cv. Isabella leaves in V79 cells.
abstract: Grapes are one of the most commonly consumed fruit, in both fresh and processed forms; however, a significant amount is disposed of in the environment. Searching for a use of this waste, the antigenotoxic, antimutagenic, and antioxidant activities of aqueous extracts from organic and conventional Vitis labrusca leaves were determined using V79 cells as model. The antigenotoxic activity was analyzed by the alkaline comet assay using endonuclease III and formamidopyrimidine DNA glycosylase enzymes. The antimutagenic property was assessed through the micronucleus (MN) formation, and antioxidant activities were assessed using 2',7'-dichlorodihydrofluorescin diacetate (DCFH-DA) assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH(●)) radical scavenging, as well as with superoxide dismutase (SOD) and catalase (CAT) activity assays. In addition, phenolic content and ascorbic acid levels of both extracts were determined. Data showed that both organic and conventional grapevine leaves extracts possessed antigenotoxic and antimutagenic properties. The extract of organic leaves significantly reduced intracellular reactive oxygen species (ROS) levels in V79 cells, and displayed greater ability for DPPH(●) scavenging and higher SOD and CAT activities than extract from conventional leaves. Further, the extract from organic leaves contained higher phenolic and ascorbic acid concentrations. In summary, extracts from organic and conventional grape leaves induced important in vitro biological effects.
authors: a Departamento de Biofísica/Centro de Biotecnologia , Instituto de Biociências, Universidade Federal do Rio Grande do Sul) , Porto Alegre , Rio Grande do Sul , Brazil.|b Unidad de Investigación, Innovación y Desarrollo en Genética y Biología Molecular , Universidad Simón Bolívar , Barranquilla , Colombia.
authors: Cristiano
authors: C
authors: Trindade
authors: c Instituto de Biotecnologia, Universidade de Caxias do Sul , Caxias do Sul , Rio Grande do Sul , Brazil.
authors: Giovana Vera
authors: GV
authors: Bortolini
authors: c Instituto de Biotecnologia, Universidade de Caxias do Sul , Caxias do Sul , Rio Grande do Sul , Brazil.
authors: Bárbara Segalotto
authors: BS
authors: Costa
authors: c Instituto de Biotecnologia, Universidade de Caxias do Sul , Caxias do Sul , Rio Grande do Sul , Brazil.
authors: Joanna Carra
authors: JC
authors: Anghinoni
authors: a Departamento de Biofísica/Centro de Biotecnologia , Instituto de Biociências, Universidade Federal do Rio Grande do Sul) , Porto Alegre , Rio Grande do Sul , Brazil.
authors: Temenouga Nikolova
authors: TN
authors: Guecheva
authors: d Cátedra de Farmacognosia y Productos Naturales, Facultad de Química , Universidad de la República , Montevideo , Uruguay.
authors: Ximena
authors: X
authors: Arias
authors: d Cátedra de Farmacognosia y Productos Naturales, Facultad de Química , Universidad de la República , Montevideo , Uruguay.
authors: Maria Verónica
authors: MV
authors: Césio
authors: d Cátedra de Farmacognosia y Productos Naturales, Facultad de Química , Universidad de la República , Montevideo , Uruguay.
authors: Horácio
authors: H
authors: Heinzen
authors: e Laboratório de Genética Toxicológica , Universidade Federal de Ciências da Saúde de Porto Alegre , Porto Alegre , Rio Grande do Sul , Brazil.
authors: Dinara Jaqueline
authors: DJ
authors: Moura
authors: e Laboratório de Genética Toxicológica , Universidade Federal de Ciências da Saúde de Porto Alegre , Porto Alegre , Rio Grande do Sul , Brazil.
authors: Jenifer
authors: J
authors: Saffi
authors: c Instituto de Biotecnologia, Universidade de Caxias do Sul , Caxias do Sul , Rio Grande do Sul , Brazil.
authors: Mirian
authors: M
authors: Salvador
authors: a Departamento de Biofísica/Centro de Biotecnologia , Instituto de Biociências, Universidade Federal do Rio Grande do Sul) , Porto Alegre , Rio Grande do Sul , Brazil.|c Instituto de Biotecnologia, Universidade de Caxias do Sul , Caxias do Sul , Rio Grande do Sul , Brazil.
authors: João Antonio Pêgas
authors: JA
authors: Henriques
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000032:analysis*; D002460:Cell Line; D003412:Cricetulus; D015162:Micronucleus Tests; D058871:Organic Agriculture; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis*; D027843:Vitis / Q000737:chemistry*
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D001205:Ascorbic Acid