title: Residual neurogenesis in chronically epileptic hippocampus of mice.
abstract: Abnormal hippocampal neurogenesis after acute seizures has been well addressed. However, whether newly generated cells continued to be disturbed even they were born in the chronic stage after pilocarpine-induce status epilepticus has remained elusive. Labeling dividing progenitors and their progeny with retroviral vector expressing green fluorescent protein or proliferation marker 5-bromo-2'-deoxyuridine at 3 months post pilocarpine-induced status epilepticus in mice, a spot of newly born neurons exhibiting hilar ectopic location (4.57±2.3%), aberrant basal dendrites (8.09±1.5%) and abnormal axon sprouting into inner molecular layer of dentate gyrus was identified when examined 6 weeks later. No significant difference on the amount of mossy fiber sprouting was found when cohorts of newborn cells were eliminated by methylazoxymethanol acetate injection initiated at 3 months after SE, suggesting that adult generated neurons in the chronically epileptic hippocampus don't contribute a lot to the mossy fiber sprouting. These results indicated that the aberrant neurogenesis in the chronically epileptic hippocampus occurs only in a small population of newly generated granule cells.
authors: Institute of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an city,710061, China.
authors: Jian-Xin
authors: JX
authors: Liu
authors: Institute of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an city,710061, China; Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an city, 710061, China.
authors: Ming
authors: M
authors: Hu
authors: Institute of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an city,710061, China.
authors: Kun
authors: K
authors: Zhu
authors: Institute of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an city,710061, China; Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an city, 710061, China.
authors: Xin-Lin
authors: XL
authors: Chen
authors: Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an city, 710061, China.
authors: Jian-Shui
authors: JS
authors: Zhang
authors: Institute of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an city,710061, China.
authors: Bo
authors: B
authors: Yuan
authors: Institute of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an city,710061, China. Electronic address: liuy5599@mail.xjtu.edu.cn.
authors: Yong
authors: Y
authors: Liu
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002465:Cell Movement; D002908:Chronic Disease; D004195:Disease Models, Animal; D004827:Epilepsy / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D005455:Fluorescent Antibody Technique; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology*; D051379:Mice; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000473:pathology / Q000502:physiology; D010862:Pilocarpine
chemical_list: D010862:Pilocarpine; D049452:Green Fluorescent Proteins; D001973:Bromodeoxyuridine
keywords: Basal dendrite; Ectopic granular cell; Epilepsy; Mossy fiber sprouting; Neurogenesis