title: Effect of Prolyl-Glycyl-Proline (PGP) and Its Acetylated Form (N-AcPGP) on Calcium Level in the Cytoplasm of Rat Peritoneal Mast Cells.
abstract: Tripeptide glycyl-prolyl-proline (PGP), a regulatory peptide of the glyproline family, possesses a pronounced anti-inflammatory effect primarily due to its ability to prevent secretion of the proinflammatory mediator histamine by rat peritoneal mast cells. Activation of mast cell with synacthen (ACTH1-24) and substance 48/80 leads to an increase in intracellular calcium concentration. Pretreatment of mast cells with PGP prevented calcium entry into the cytoplasm from both intercellular space and intracellular stores. Acetylated peptide (N-AcPGP) produced a similar effect on histamine release and intracellular calcium content in mast cells activated with synacthen. These findings indicate that both forms of the peptide can stabilize mast cells and prevent intracellular calcium increase.
authors: N. K. Koltsov Institute of Developmental Biology of the Russian Academy of Sciences, Moscow, Russia. n.s.bondarenko@gmail.com.
authors: N S
authors: NS
authors: Bondarenko
authors: M. V. Lomonosov Moscow State University, Moscow, Russia.
authors: A D
authors: AD
authors: Kurenkova
authors: N. K. Koltsov Institute of Developmental Biology of the Russian Academy of Sciences, Moscow, Russia.|M. V. Lomonosov Moscow State University, Moscow, Russia.
authors: D A
authors: DA
authors: Nikishin
authors: M. V. Lomonosov Moscow State University, Moscow, Russia.
authors: B A
authors: BA
authors: Umarova
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D011392:Proline / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D051381:Rats
chemical_list: D009842:Oligopeptides; C113164:prolyl-glycyl-proline; D011392:Proline; D002118:Calcium
keywords: intracellular calcium; mast cells; prolyl-glycyl-proline (PGP)