title: Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway.
abstract: Wnt pathways are critical for embryonic development and adult tissue homeostasis in all multicellular animals. Many regulatory mechanisms exist to control proper signaling output. Recent studies suggest that cell surface Wnt receptor level is controlled by ubiquitination, and serve as a critical regulatory point of Wnt pathway activity as it determines the responsiveness of cells to Wnt signal. Here, we describe flow cytometry, cell surface protein biotinylation, and immunofluorescence pulse-chase methods to probe the surface expression, ubiquitination, and internalization of the Wnt receptors FZD and LRP6.
authors: Immuno-oncology, Novartis Institutes for Biomedical Research, Cambridge, MA, USA.
authors: Xiaomo
authors: X
authors: Jiang
authors: Developmental and Molecular Pathways, Novartis Institutes for Biomedical Research, 250 Massachusetts Avenue, Cambridge, MA, 02139, USA. feng.cong@novartis.com.
authors: Feng
authors: F
authors: Cong
mesh_terms: D000818:Animals; D019921:Biotinylation; D002462:Cell Membrane / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D011247:Pregnancy; D060490:Receptors, Wnt / Q000737:chemistry* / Q000378:metabolism; D051153:Wnt Proteins / Q000737:chemistry* / Q000378:metabolism; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000737:chemistry / Q000378:metabolism
chemical_list: D060490:Receptors, Wnt; D051153:Wnt Proteins; D051176:beta Catenin
keywords: Cell surface protein biotinylation; FZD; Flow cytometry; Immunofluorescence; LRP6; Pulse-chase; Receptor; Wnt