title: Treatment of raw and ozonated oil sands process-affected water under decoupled denitrifying anoxic and nitrifying aerobic conditions: a comparative study.
abstract: Batch experiments were performed to evaluate biodegradation of raw and ozonated oil sands process-affected water (OSPW) under denitrifying anoxic and nitrifying aerobic conditions for 33 days. The results showed both the anoxic and aerobic conditions are effective in degrading OSPW classical and oxidized naphthenic acids (NAs) with the aerobic conditions demonstrating higher removal efficiency. The reactors under nitrifying aerobic condition reduced the total classical NAs of raw OSPW by 69.1 %, with better efficiency for species of higher hydrophobicity. Compared with conventional aerobic reactor, nitrifying aerobic condition substantially shortened the NA degradation half-life to 16 days. The mild-dose ozonation remarkably accelerated the subsequent aerobic biodegradation of classical NAs within the first 14 days, especially for those with long carbon chains. Moreover, the ozone pretreatment enhanced the biological removal of OSPW classical NAs by leaving a considerably lower final residual concentration of 10.4 mg/L under anoxic conditions, and 5.7 mg/L under aerobic conditions. The combination of ozonation and nitrifying aerobic biodegradation removed total classical NAs by 76.5 % and total oxy-NAs (O3-O6) by 23.6 %. 454 Pyrosequencing revealed that microbial species capable of degrading recalcitrant hydrocarbons were dominant in all reactors. The most abundant genus in the raw and ozonated anoxic reactors was Thauera (~56 % in the raw OSPW anoxic reactor, and ~65 % in the ozonated OSPW anoxic reactor); whereas Rhodanobacter (~40 %) and Pseudomonas (~40 %) dominated the raw and ozonated aerobic reactors, respectively. Therefore, the combination of mild-dose ozone pretreatment and subsequent biological process could be a competent choice for OSPW treatment.
authors: Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada.
authors: Jinkai
authors: J
authors: Xue
authors: Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada.
authors: Yanyan
authors: Y
authors: Zhang
authors: Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada. yang.liu@ualberta.ca.
authors: Yang
authors: Y
authors: Liu
authors: Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada. mgamalel-din@ualberta.ca.
authors: Mohamed
authors: M
authors: Gamal El-Din
mesh_terms: D000332:Aerobiosis; D001673:Biodegradation, Environmental; D002264:Carboxylic Acids / Q000378:metabolism; D058440:Denitrification; D058465:Nitrification; D061329:Oil and Gas Fields; D010126:Ozone / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D018508:Water Purification / Q000379:methods*
chemical_list: D002264:Carboxylic Acids; D014874:Water Pollutants, Chemical; D010126:Ozone; C046170:naphthenic acid
keywords: Denitrification; Microbial community; Naphthenic acids; Nitrification; Ozonation