title: MiR124 suppresses collagen formation of human tendon derived stem cells through targeting egr1.
abstract: Collagen formation is used as a crucial indicator of tenogenic differentiation of human tendon derived stem cell (hTDSC). Early growth response-1(egr1), a transcriptional factor, has been demonstrated to regulate tendon differentiation and promote tendon repair. Considering that the therapeutic options for tendon injuries remain limited, investigating the regulation of egr1 could facilitate the understanding of tendon development at molecular level so as to find a promising therapeutic target. MicroRNAs (miRNA) have been considered as epigenetic regulators to mediate multiple biological activities including stem cell differentiation. In the present study, biological experiments confirmed the prediction that miR124-3p (miR124) could have direct binding with egr1. We also found that miR124 suppressed collagen formation during the tendon differentiation of hTDSC while anti-miR124 promoted it. Furthermore, egr1 knockdown abolished the promotive effect of anti-miR124, suggesting that miR124 prevents tendon differentiation via suppressing egr1 expression. Therefore, miR124 may be a promising therapeutic target for tendon injury.
authors: Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China; The Chinese University of Hong Kong Shenzhen Research Institute, Shenzhen, PR China.
authors: Bin
authors: B
authors: Wang
authors: Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China.
authors: Jia
authors: J
authors: Guo
authors: Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China.
authors: Lu
authors: L
authors: Feng
authors: Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China.
authors: Chun-Wai
authors: CW
authors: Suen
authors: School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, PR China. Electronic address: fuweiming76@smu.edu.cn.
authors: Wei-Ming
authors: WM
authors: Fu
authors: Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China; The Chinese University of Hong Kong Shenzhen Research Institute, Shenzhen, PR China.
authors: Jin-Fang
authors: JF
authors: Zhang
authors: Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, PR China; The Chinese University of Hong Kong Shenzhen Research Institute, Shenzhen, PR China. Electronic address: gangli@cuhk.edu.hk.
authors: Gang
authors: G
authors: Li
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D002454:Cell Differentiation; D003094:Collagen / Q000378:metabolism*; D051766:Early Growth Response Protein 1 / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D013710:Tendons / Q000166:cytology*
chemical_list: D020413:3' Untranslated Regions; C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; C509207:MIRN124 microRNA, human; D035683:MicroRNAs; D003094:Collagen
keywords: Early growth response-1; Human tendon derived stem cell; Microrna; Tendon differentiation