title: Comparison of amniotic fluid matrix metalloproteinase-8 and cathelicidin in the diagnosis of intra-amniotic infection.
abstract: To evaluate the association of amniotic fluid (AF) matrix metalloproteinase-8 (MMP-8) and cathelicidin concentrations with microbial invasion of the amniotic cavity (MIAC) in pregnancies with preterm prelabor rupture of the membranes or intact membranes.
  
  
  
  Amniocentesis was performed in 54 singleton pregnancies between 22+0and 34+2gestational weeks with suspected intra-amniotic infection. AF-MMP-8 was analysed by immunoassay and AF-cathelicidin by commercial ELISA. Standard biochemical methods, molecular microbiology and culture techniques were used.
  
  
  
  MIAC was present in 18 (33%) women. The cutoff value for the diagnosis of MIAC was 41.5 ng ml-1for AF-MMP-8, and 11.6 ng ml-1for AF-cathelicidin. With these cutoff values AF-MMP-8 had a sensitivity of 100%, specificity of 69%, positive predictive value of 62% and negative predictive value of 100% for MIAC. The corresponding values for AF-cathelicidin were 89, 81, 70 and 94%.
  
  
  
  The performance of AF-cathelicidin in the prediction of MIAC is comparable to AF-MMP-8.
authors: Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: T
authors: T
authors: Myntti
authors: Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: L
authors: L
authors: Rahkonen
authors: Department of Bacteriology, University of Helsinki and Helsinki University Hospital, HUSLAB, Helsinki, Finland.
authors: A
authors: A
authors: Pätäri-Sampo
authors: Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: M
authors: M
authors: Tikkanen
authors: Department of Oral and Maxillofacial Diseases, Helsinki University Hospital, Institute of Dentistry, University of Helsinki, Helsinki, Finland.|Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.
authors: T
authors: T
authors: Sorsa
authors: Medix Biochemica, Espoo, Finland.
authors: J
authors: J
authors: Juhila
authors: Children's Hospital, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: O
authors: O
authors: Helve
authors: Children's Hospital, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: S
authors: S
authors: Andersson
authors: Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: J
authors: J
authors: Paavonen
authors: Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: V
authors: V
authors: Stefanovic
mesh_terms: D000328:Adult; D000649:Amniocentesis; D000653:Amniotic Fluid / Q000737:chemistry* / Q000201:enzymology / Q000382:microbiology; D023181:Antimicrobial Cationic Peptides / Q000032:analysis* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D002821:Chorioamnionitis / Q000201:enzymology / Q000378:metabolism; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000175:diagnosis* / Q000201:enzymology; D005865:Gestational Age; D006801:Humans; D020784:Matrix Metalloproteinase 8 / Q000032:analysis* / Q000378:metabolism; D007752:Obstetric Labor, Premature / Q000201:enzymology; D011237:Predictive Value of Tests; D011247:Pregnancy; D011446:Prospective Studies; D012372:ROC Curve; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D015415:Biomarkers; D020784:Matrix Metalloproteinase 8; D054804:Cathelicidins