title: Candida albicans responds to glycostructure damage by Ace2-mediated feedback regulation of Cek1 signaling.
abstract: Candida albicans uses the Cek1 MAPK pathway to restore cells from damage of its cell wall glycostructures. Defective protein N- or O-glycosylation activates Cek1 and the transcription factor Ace2 as its downstream target, to upregulate genes encoding protein O-mannosyltransferases (Pmt proteins). In unstressed cells, Cek1-Ace2 activity blocks expression of PMT1, which is de-repressed by tunicamycin. Genomic binding targets of Ace2 included ZCF21, which was upregulated by Ace2 and found to repress PMT1 transcription in unstressed cells. Surprisingly, genes encoding components of the Cek1 pathway including MSB2, CST20, HST7, CEK1 and ACE2 were also identified as Ace2 targets indicating Ace2-mediated transcriptional amplification of pathway genes under N-glycosylation stress. In this condition, physical interaction of the Ace2 protein with the upstream MAPKKK Cst20 was detected. Cst20-GFP showed stress-induced import from the cytoplasm into the nucleus and phosphorylation of Ace2. Interestingly, forced nuclear localization of Cst20 inhibited Cek1-Ace2 signaling, while forced cytoplasmic localization of Cst20 retained full signaling activity, suggesting that nuclear Cst20 downregulates the Cek1 pathway. Collectively, the results indicate that Ace2 is a versatile multifunctional transcriptional regulator, which activates glycostress responses of C. albicans by both positive forward and negative feedback regulation of Cek1 signaling.
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, 40225, Düsseldorf, Germany.|Manchot Graduate School Molecules of Infection, Heinrich-Heine-Universität, 40225, Düsseldorf, Germany.
authors: Lasse
authors: L
authors: van Wijlick
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, 40225, Düsseldorf, Germany.
authors: Marc
authors: M
authors: Swidergall
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, 40225, Düsseldorf, Germany.
authors: Philipp
authors: P
authors: Brandt
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, 40225, Düsseldorf, Germany.|Manchot Graduate School Molecules of Infection, Heinrich-Heine-Universität, 40225, Düsseldorf, Germany.
authors: Joachim F
authors: JF
authors: Ernst
mesh_terms: D002176:Candida albicans / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D008364:Mannosyltransferases / Q000235:genetics / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010766:Phosphorylation; D011497:Protein O-Methyltransferase / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics
chemical_list: C505770:ACE2 protein, Candida glabrata; D005656:Fungal Proteins; D014157:Transcription Factors; C077339:CEK1 protein, Candida albicans; D011497:Protein O-Methyltransferase; D008364:Mannosyltransferases; C082696:protein O-mannosyltransferase; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases