title: The level of the zinc homeostasis regulating proteins in the brain of rats subjected to olfactory bulbectomy model of depression.
abstract: Zinc transporters (ZnTs) and metallothioneins (MT) are important in maintaining Zn homeostasis in the brain. The present study was designed to find out whether alterations in ZnTs and MTs are associated with the pathophysiology of depression and the mechanism of antidepressant action.
  
  
  
  Messenger RNA and proteins of ZnT1, ZnT3, ZnT4, ZnT5, ZnT6 and MT1/2 were measured in the prefrontal cortex (PFC) and hippocampus (Hp) of rats subjected to olfactory bulbectomy (OB) (a model of depression) and chronic amitriptyline (AMI) treatment by Real Time PCR and Western Blot/Immunohistochemistry (IHP).
  
  
  
  Results in the OB rats showed: increases in the protein levels of ZnT1 in the PFC and Hp and MT1/2 in the PFC; a decrease in ZnT3 protein level in the PFC; no changes in ZnT4, ZnT5 and ZnT6 in the PFC and Hp. IHP labeling revealed increases in the optical densities of ZnT1-IR in the PFC and Hp and decreases in ZnT3 and ZnT4-IR in the PFC of OB rats. Although OB had no effects on gene expression of ZnTs, mRNAs for MT1/2 were increased. Chronic AMI treatment did not influence protein levels of ZnTs and MT1/2 in Sham and OB rats; however decreased mRNA levels of ZnT4 and ZnT5 in PFC and ZnT1, ZnT3, ZnT4 and ZnT6 in Hp of Sham rats and normalized OB induced increase in MT1/2 gene expression.
  
  
  
  Changes in ZnTs and MT1/2 suggest altered cortical distribution of Zn in the OB model which further supports the hypothesis that Zn dyshomeostasis may be involved in the pathophysiology of depression.
authors: Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland; Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland.
authors: Anna
authors: A
authors: Rafalo
authors: Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, PL 30-688, Krakow, Poland.
authors: Monika
authors: M
authors: Zadrozna
authors: Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, PL 30-688, Krakow, Poland.
authors: Barbara
authors: B
authors: Nowak
authors: Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland.
authors: Katarzyna
authors: K
authors: Kotarska
authors: Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland.
authors: Katarzyna
authors: K
authors: Wiatrowska
authors: Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland.
authors: Bartlomiej
authors: B
authors: Pochwat
authors: Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland.
authors: Magdalena
authors: M
authors: Sowa-Kucma
authors: Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland; Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, PL 30-688, Krakow, Poland.
authors: Paulina
authors: P
authors: Misztak
authors: Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland; Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, PL 30-688, Krakow, Poland.
authors: Gabriel
authors: G
authors: Nowak
authors: Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland. Electronic address: szewczyk@if-pan.krakow.pl.
authors: Bernadeta
authors: B
authors: Szewczyk
mesh_terms: D000639:Amitriptyline / Q000627:therapeutic use; D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000627:therapeutic use; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003863:Depression / Q000188:drug therapy / Q000209:etiology / Q000473:pathology*; D004195:Disease Models, Animal; D005106:Exploratory Behavior / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism; D009830:Olfactory Bulb / Q000601:surgery*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015032:Zinc / Q000378:metabolism*
chemical_list: D000929:Antidepressive Agents, Tricyclic; D002352:Carrier Proteins; D012333:RNA, Messenger; C017800:zinc-binding protein; D000639:Amitriptyline; D008668:Metallothionein; D015032:Zinc
keywords: Depression; Metallothioneins; Olfactory bulbectomy; Zinc; Zinc transporter