title: Enhanced stability and activity of an antimicrobial peptide in conjugation with silver nanoparticle.
abstract: The conjugation of nanoparticles with antimicrobial peptides (AMP) is emerging as a promising route to achieve superior antimicrobial activity. However, the nature of peptide-nanoparticle interactions in these systems remains unclear. This study describes a system consisting of a cysteine containing antimicrobial peptide conjugated with silver nanoparticles, in which the two components exhibit a dynamic interaction resulting in a significantly enhanced stability and biological activity compared to that of the individual components. This was investigated using NMR spectroscopy in conjunction with other biophysical techniques. Using fluorescence assisted cell sorting and membrane mimics we carried out a quantitative comparison of the activity of the AMP-nanoparticle system and the free peptide. Taken together, the study provides new insights into nanoparticle-AMP interactions at a molecular level and brings out the factors that will be useful for consideration while designing new conjugates with enhanced functionality.
authors: NMR Research Centre, Indian Institute of Science, Bangalore 560012, India; Solid State and Structural Chemistry Unit, Indian Institute of Science, Bangalore 560012, India.
authors: Indrani
authors: I
authors: Pal
authors: NMR Research Centre, Indian Institute of Science, Bangalore 560012, India.
authors: Varsha P
authors: VP
authors: Brahmkhatri
authors: Department of Biophysics, Bose Institute, Kolkata 700054, India.
authors: Swapna
authors: S
authors: Bera
authors: Department of Biophysics, Bose Institute, Kolkata 700054, India.
authors: Dipita
authors: D
authors: Bhattacharyya
authors: Molecular Reproduction Development and Genetics, Indian Institute of Science, Bangalore 560012, India.
authors: Yasrib
authors: Y
authors: Quirishi
authors: Department of Biophysics, Bose Institute, Kolkata 700054, India. Electronic address: bhunia@jcbose.ac.in.
authors: Anirban
authors: A
authors: Bhunia
authors: NMR Research Centre, Indian Institute of Science, Bangalore 560012, India. Electronic address: hsatreya@sif.iisc.ernet.in.
authors: Hanudatta S
authors: HS
authors: Atreya
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000494:pharmacology; D040761:Biomimetic Materials / Q000737:chemistry; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D004355:Drug Stability; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D050296:Microbial Viability / Q000187:drug effects; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D053835:Unilamellar Liposomes / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D053835:Unilamellar Liposomes; C057561:1-palmitoyl-2-oleoylphosphatidylethanolamine; D012834:Silver; C060037:1-palmitoyl-2-oleoylglycero-3-phosphoglycerol; D003545:Cysteine
keywords: Antimicrobial peptide; Fast chemical exchange; Membrane interaction; NMR spectroscopy; Peptide nanoparticle conjugate; Silver nanoparticle