title: Truncated Autoinducing Peptides as Antagonists of Staphylococcus lugdunensis Quorum Sensing.
abstract: Competitive quorum sensing (QS) antagonism offers a novel strategy for attenuating current multidrug resistant staphylococcal infections. To this end, a series of 10 truncated analogues based on the parent autoinducing peptides (AIPs) of Staphylococcus lugdunensis (groups I and II) and Staphylococcus epidermidis (groups I-III) were sequentially assessed against a newly developed Staphylococcus lugdunensis group I QS reporter strain. The truncated analogues based upon Staphylococcus lugdunensis AIP-1 (1) and AIP-2 (2) displayed respective IC50values of 0.2 ± 0.01 μM and 0.3 ± 0.01 μM, while the truncated analogue of the Staphylococcus epidermidis AIP-1 (3) elicited an IC50value of 2.7 ± 0.1 μM. These findings demonstrate the potential of cognate and "crosstalk" competitive quorum sensing inhibition using truncated AIPs as a means of attenuating staphylococcal infections in species beyond Staphylococcus aureus.
authors: School of Science and Health, Western Sydney University , Locked Bag 1797, Penrith South Dc, NSW 2751, Australia.
authors: Christopher P
authors: CP
authors: Gordon
authors: Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa , Iowa City, Iowa 52242, United States.
authors: Shondra D
authors: SD
authors: Olson
authors: Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa , Iowa City, Iowa 52242, United States.
authors: Jessica L
authors: JL
authors: Lister
authors: Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa , Iowa City, Iowa 52242, United States.
authors: Jeffrey S
authors: JS
authors: Kavanaugh
authors: Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa , Iowa City, Iowa 52242, United States.
authors: Alexander R
authors: AR
authors: Horswill
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000494:pharmacology*; D055808:Drug Discovery; D006801:Humans; D008958:Models, Molecular; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000494:pharmacology*; D053038:Quorum Sensing / Q000187:drug effects*; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D013212:Staphylococcus epidermidis / Q000737:chemistry; D057787:Staphylococcus lugdunensis / Q000187:drug effects* / Q000502:physiology
chemical_list: C414532:AgrD protein, Staphylococcus; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010456:Peptides, Cyclic