title: High fat diet drives obesity regardless the composition of gut microbiota in mice.
abstract: The gut microbiota is involved in many aspects of host physiology but its role in body weight and glucose metabolism remains unclear. Here we studied the compositional changes of gut microbiota in diet-induced obesity mice that were conventionally raised or received microbiota transplantation. In conventional mice, the diversity of the faecal microbiota was weakly associated with 1(st) week weight gain but transferring the microbiota of mice with contrasting weight gain to germfree mice did not change obesity development or feed efficiency of recipients regardless whether the microbiota was taken before or after 10 weeks high fat (HF) feeding. Interestingly, HF-induced glucose intolerance was influenced by microbiota inoculation and improved glucose tolerance was associated with a low Firmicutes to Bacteroidetes ratio. Transplantation of Bacteroidetes rich microbiota compared to a control microbiota ameliorated glucose intolerance caused by HF feeding. Altogether, our results demonstrate that gut microbiota is involved in the regulation of glucose metabolism and the abundance of Bacteroidetes significantly modulates HF-induced glucose intolerance but has limited impact on obesity in mice. Our results suggest that gut microbiota is a part of complex aetiology of insulin resistance syndrome, individual microbiota composition may cause phenotypic variation associated with HF feeding in mice.
authors: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
authors: Sylvie
authors: S
authors: Rabot
authors: Nestlé Research Centre, Lausanne, Switzerland.
authors: Mathieu
authors: M
authors: Membrez
authors: Nestlé Research Centre, Lausanne, Switzerland.
authors: Florence
authors: F
authors: Blancher
authors: Nestlé Research Centre, Lausanne, Switzerland.
authors: Bernard
authors: B
authors: Berger
authors: Nestlé Research Centre, Lausanne, Switzerland.|Nestlé Institute of Health Sciences, Lausanne, Switzerland.
authors: Déborah
authors: D
authors: Moine
authors: Nestlé Research Centre, Lausanne, Switzerland.
authors: Lutz
authors: L
authors: Krause
authors: Nestlé Research Centre, Lausanne, Switzerland.
authors: Rodrigo
authors: R
authors: Bibiloni
authors: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
authors: Aurélia
authors: A
authors: Bruneau
authors: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
authors: Philippe
authors: P
authors: Gérard
authors: Nestlé Institute of Health Sciences, Lausanne, Switzerland.
authors: Jay
authors: J
authors: Siddharth
authors: Nestlé Institute of Health Sciences, Lausanne, Switzerland.
authors: Christian L
authors: CL
authors: Lauber
authors: Nestlé Research Centre, Lausanne, Switzerland.|Nestlé Institute of Health Sciences, Lausanne, Switzerland.
authors: Chieh Jason
authors: CJ
authors: Chou
mesh_terms: D000818:Animals; D041963:Bacteroidetes / Q000145:classification / Q000254:growth & development; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000009:adverse effects*; D000069467:Fecal Microbiota Transplantation; D000068536:Bacillota / Q000145:classification / Q000254:growth & development; D000069196:Gastrointestinal Microbiome / Q000502:physiology; D018149:Glucose Intolerance / Q000209:etiology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D020560:Proteobacteria / Q000145:classification / Q000254:growth & development
chemical_list: D004041:Dietary Fats