title: Morphological changes of the red blood cells treated with metal oxide nanoparticles.
abstract: The toxic effect of Al2O3, SiО2and ZrО2nanoparticles on red blood cells of Wistar rats was studied in vitro using the atomic force microscopy and the fluorescence analysis. Transformation of discocytes into echinocytes and spherocytes caused by the metal oxide nanoparticles was revealed. It was shown that only extremely high concentration of the nanoparticles (2mg/ml) allows correct estimating of their effect on the cell morphology. Besides, it was found out that the microviscosity changes of red blood cell membranes treated with nanoparticles began long before morphological modifications of the cells. On the contrary, the negatively charged ZrO2and SiO2nanoparticles did not affect ghost microviscosity up to concentrations of 1μg/ml and 0.1mg/ml, correspondingly. In its turn, the positively charged Al2O3nanoparticles induced structural changes in the lipid bilayer of the red blood cells already at a concentration of 0.05μg/ml. A decrease in microviscosity of the erythrocyte ghosts treated with Al2O3and SiO2nanoparticles was shown. It was detected that the interaction of ZrO2nanoparticles with the cells led to an increase in the membrane microviscosity and cracking of swollen erythrocytes.
authors: Institute of Strength Physics and Materials Science of SB RAS, Tomsk 634055, Russia. Electronic address: annakozelskaya@gmail.com.
authors: A I
authors: AI
authors: Kozelskaya
authors: Institute of Strength Physics and Materials Science of SB RAS, Tomsk 634055, Russia; National Research Tomsk Polytechnic University, Tomsk 634050, Russia.
authors: A V
authors: AV
authors: Panin
authors: National Research Tomsk Polytechnic University, Tomsk 634050, Russia; Siberian State Medical University, Tomsk 634050, Russia.
authors: I A
authors: IA
authors: Khlusov
authors: Scientific Research Institute of Biochemistry of SB RAMS, Novosibirsk 630117, Russia.
authors: P V
authors: PV
authors: Mokrushnikov
authors: SRC VB Vector, Koltsovo, Novosibirsk Region 630559, Russia.
authors: B N
authors: BN
authors: Zaitsev
authors: Siberian State Medical University, Tomsk 634050, Russia.
authors: D I
authors: DI
authors: Kuzmenko
authors: Siberian State Medical University, Tomsk 634050, Russia.
authors: G Yu
authors: GY
authors: Vasyukov
mesh_terms: D000537:Aluminum Oxide / Q000633:toxicity*; D000818:Animals; D004912:Erythrocytes / Q000187:drug effects* / Q000473:pathology; D053768:Metal Nanoparticles / Q000633:toxicity*; D018625:Microscopy, Atomic Force; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000633:toxicity*; D015040:Zirconium / Q000633:toxicity*
chemical_list: D012822:Silicon Dioxide; D015040:Zirconium; D000537:Aluminum Oxide; C028541:zirconium oxide
keywords: AFM; Fluorescence analysis; In vitro; Membrane; Metal oxide nanoparticle; Red blood cells