title: Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples.
abstract: Diuron is one of the most widely herbicide used worldwide, which can undergo degradation producing three primary metabolites: 3,4-dichlorophenylurea, 3-(3,4-dichlorophenyl)-1-methylurea, and 3,4-dichloroaniline. Since the persistence of diuron and its by-products in ecosystems involves risk of toxicity to environment and human health, a reliable quantitative method for simultaneous monitoring of these compounds is required. Hence, a simple method without preconcentration step was validated for quantitation of diuron and its main metabolites by high performance liquid chromatography with ultraviolet detection. Separation was achieved in less than 11 minutes using a C18 column, mobile phase composed of acetonitrile and water (45:55 v/v) at 0.86 mL min-1 and detection at 254 nm. The validated method using solid-liquid extraction followed by an isocratic chromatographic elution proved to be specific, precise and linear (R2 ˃ 0.99), presenting more than 90% of recovery. The method was successfully applied to quantify diuron and their by-products in soil samples collected in a sugarcane cultivation area, focusing on the environmental control.
authors: Universidade Estadual de Londrina, Departamento de Química, Rod. Celso Garcia Cid, Km 380, 86057-970 Londrina, PR, Brasil.
authors: Ana Lucia S M
authors: AL
authors: Felicio
authors: Universidade Estadual de Londrina, Departamento de Química, Rod. Celso Garcia Cid, Km 380, 86057-970 Londrina, PR, Brasil.
authors: Alessandra M
authors: AM
authors: Monteiro
authors: Universidade Estadual de Londrina, Departamento de Química, Rod. Celso Garcia Cid, Km 380, 86057-970 Londrina, PR, Brasil.
authors: Mariana B
authors: MB
authors: Almeida
authors: Universidade Estadual de Londrina, Departamento de Química, Rod. Celso Garcia Cid, Km 380, 86057-970 Londrina, PR, Brasil.
authors: Tiago B
authors: TB
authors: Madeira
authors: Universidade Estadual de Londrina, Departamento de Química, Rod. Celso Garcia Cid, Km 380, 86057-970 Londrina, PR, Brasil.
authors: Suzana L
authors: SL
authors: Nixdorf
authors: Universidade Estadual de Londrina, Departamento de Química, Rod. Celso Garcia Cid, Km 380, 86057-970 Londrina, PR, Brasil.
authors: Maria Josefa S
authors: MJ
authors: Yabe
mesh_terms: D000814:Aniline Compounds / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004237:Diuron / Q000032:analysis*; D006540:Herbicides / Q000032:analysis*; D010671:Phenylurea Compounds / Q000032:analysis; D012987:Soil / Q000737:chemistry*
chemical_list: C561340:3,4-dichlorophenylurea; D000814:Aniline Compounds; D006540:Herbicides; D010671:Phenylurea Compounds; D012987:Soil; C014464:3,4-dichloroaniline; D004237:Diuron