title: Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
abstract: An exopolysaccharide (EPS) from probiotics Lactobacillus plantarum ZDY2013 was purified to illustrate its molecular weight, monosaccharide composition and biological activities. The yield of EPS (429.4±30.3mg/L) was obtained with a purity of 96.06%. The EPS was characterized to have only one symmetrical sharp peak by high-performance size-exclusion chromatography and its molecular weight was 5.17×10(4)Da. The GC analysis revealed that EPS only consisted of xylose and galactose, and the galactose possessed as high as 98.3% (w/w) of the total monosaccharides. By sulfonation, a sulfated EPS was successfully synthesized with the degree of substitution (DS) of 0.29, which was confirmed using FT-IR spectroscopy. Both EPS and sulfated EPS showed radical scavenging activities, and the antioxidant activities increased after sulfonation. In addition, sulfated EPS was more effective in counteracting the cytotoxicity induced by B. cereus enterotoxins on Caco-2 cells when compared with EPS. In summary, sulfonation is a feasible strategy for improving the biological activities of EPS from L. plantarum ZDY203.
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, PR China.
authors: Zhihong
authors: Z
authors: Zhang
authors: Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, PR China.
authors: Zhengqi
authors: Z
authors: Liu
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
authors: Xueying
authors: X
authors: Tao
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, PR China. Electronic address: weihua@ncu.edu.cn.
authors: Hua
authors: H
authors: Wei
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry* / Q000494:pharmacology*; D013431:Sulfates / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D011135:Polysaccharides, Bacterial; D020011:Protective Agents; D013431:Sulfates
keywords: Biological activity; Characterization; Exopolysaccharides; Lactobacillus plantarum ZDY2013; Sulfonation