title: Exploring the potential of laser capture microdissection technology in integrated oral biosciences.
abstract: Laser capture microdissection (LCM) is a high-end research and diagnostic technology that helps in obtaining pure cell populations for the purpose of cell- or lesion-specific genomic and proteomic analysis. Literature search on the application of LCM in oral tissues was made through PubMed. There is ample evidence to substantiate the utility of LCM in understanding the underlying molecular mechanism involving an array of oral physiological and pathological processes, including odontogenesis, taste perception, eruptive tooth movement, oral microbes, and cancers of the mouth and jaw tumors. This review is aimed at exploring the potential application of LCM in oral tissues as a high-throughput tool for integrated oral sciences. The indispensable application of LCM in the construction of lesion-specific genomic libraries with emphasis on some of the novel molecular markers thus discovered is also highlighted.
authors: Department of Oral Pathology, Manipal College of Dental Sciences, Manipal, Karnataka, India.
authors: A
authors: A
authors: Thennavan
authors: Pacific Academy of Higher Education and Research (PAHER) University, Udaipur, Rajasthan, India.|Department of Oral Pathology, ITS Dental College, Hospital and Research Center, Greater Noida, India.
authors: M
authors: M
authors: Sharma
authors: Department of Oral Pathology, Manipal College of Dental Sciences, Manipal, Karnataka, India.
authors: C
authors: C
authors: Chandrashekar
authors: School of Clinical Dentistry, The University of Sheffield, Sheffield, UK.
authors: K
authors: K
authors: Hunter
authors: Department of Oral Pathology, Manipal College of Dental Sciences, Manipal, Karnataka, India.|School of Clinical Dentistry, The University of Sheffield, Sheffield, UK.
authors: R
authors: http://orcid.org/0000-0003-0088-4777
authors: R
authors: Radhakrishnan
mesh_terms: D023281:Genomics; D006801:Humans; D061346:Laser Capture Microdissection; D009055:Mouth / Q000196:embryology* / Q000378:metabolism*; D009059:Mouth Diseases / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D013048:Specimen Handling
keywords: laser capture microdissection; oral embryology; oral physiology; oral squamous cell carcinoma