title: Fas/FasL gene polymorphism in patients with Hashimoto's thyroiditis in Turkish population.
abstract: OBJECTIVE
  Hashimoto's disease is a polygenic disorder with complex etiopathogenesis. Apoptosis is proposed as one of its mechanisms. The Fas/Fas ligand cascade represents a major pathway initiating apoptosis. This study aims to evaluate the influence of Fas and FasL gene polymorphism in Hashimoto's thyroiditis in Turkish population.
  
  
  METHODS
  A total of 112 patients with Hashimoto's thyroiditis and 112 cases of healthy control people were included in this study. The evaluation of genotype for Fas -670 A/G and FasL 843 C/T gene polymorphism was performed by using PCR-RFLP method.
  
  
  RESULTS
  The FAS genotype and gene allele frequency distribution did differ between the control group (AA 36.6 %, AG 50.0 %, GG 13.4 %, A 61.6 %, G 38.4 %) and the Hashimoto's thyroiditis patients (AA 21.4 %, AG 50.9 %, GG 27.7 %, A 46.9 %, G 53.1 %) (p < 0.01). The evaluation of FasL genotype and gene allele frequency did not show statistically significant difference between the patient group (CC 27.7 %, CT 45.5 %, TT 26.8 %, C 50.4 %, T 49.6 %) and control group (CC 33.9 %, CT 44.6 %, TT 21,4 %, C 56.3 %, T 43.8 %) (p > 0.05).
  
  
  CONCLUSIONS
  Gene polymorphism of Fas and G allele frequency may play a role in the regulation of apoptosis in thyroid autoimmune disorders. There is a need for further studies to clarify the genetic role of apoptosis in HT.
authors: Department of Endocrinology and Metabolic Diseases, Ege University Medical School, 35100, Bornova, Izmir, Turkey. drmerdogan61@hotmail.com.
authors: M
authors: M
authors: Erdogan
authors: Department of Endocrinology and Metabolic Diseases, Necmettin Erbakan University Medical School, Konya, Turkey.
authors: M
authors: M
authors: Kulaksizoglu
authors: Department of Internal Medicine, Numune Training and Research Hospital, Adana, Turkey.
authors: S
authors: S
authors: Ganidagli
authors: Laboratory of Molecular Medicine, Department of Pediatrics, Ege University Medical School, Bornova, Izmir, Turkey.
authors: A
authors: A
authors: Berdeli
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D050031:Hashimoto Disease / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D007118:Immunoassay; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014421:Turkey / Q000453:epidemiology; D019014:fas Receptor / Q000235:genetics*
chemical_list: D015415:Biomarkers; C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D019014:fas Receptor
keywords: Fas/FasL; Gene polymorphism; Hashimoto’s thyroiditis