title: Viability of human composite tissue model for experimental study of burns.
abstract: Experimental studies of burns are primarily performed with animal models that have important anatomical and physiological differences relative to human systems. The aim of this study was to develop a human experimental burn model using composite tissue obtained from bariatric surgery. We established a new protocol to maintain viable sections of human cutaneous and subcutaneous (sub/cutaneous) tissue in vitro. Under the conditions selected, multiparametric flow cytometry and histological analysis confirmed the viability and integrity of the human sub/cutaneous tissue for at least 5 days. Furthermore, we utilized a precision McKenna burner to inflict burns on the human tissue model under well-defined thermal conditions in vitro. Our data showed a localized, temporally restricted polarization of the resident macrophages in the subcutaneous human tissue in response to specific thermal forces. Therefore, our model provides a useful alternative to animal studies for further detailed investigations of human responses to injuries and treatments.
authors: Department of Plastic and Reconstructive Surgery, Hand Surgery and Burn Center, RWTH Aachen University Hospital, Aachen 52074, Germany.
authors: Miao
authors: M
authors: Qu
authors: Institute for Combustion Technology, RWTH Aachen University, Aachen 52056, Germany.
authors: Stephan
authors: S
authors: Kruse
authors: Institute for Combustion Technology, RWTH Aachen University, Aachen 52056, Germany.
authors: Heinz
authors: H
authors: Pitsch
authors: Department of Plastic and Reconstructive Surgery, Hand Surgery and Burn Center, RWTH Aachen University Hospital, Aachen 52074, Germany.
authors: Norbert
authors: N
authors: Pallua
authors: Department of Plastic and Reconstructive Surgery, Hand Surgery and Burn Center, RWTH Aachen University Hospital, Aachen 52074, Germany.
authors: Mahtab
authors: M
authors: Nourbakhsh
mesh_terms: D000293:Adolescent; D000328:Adult; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008962:Models, Theoretical; D055815:Young Adult