title: High Throughput siRNA Screening Using Reverse Transfection.
abstract: RNA interference (RNAi) is a commonly used technique to knockdown gene function. Here, we describe a high throughput screening method for siRNA mediated gene silencing of the breast cancer cell line MDA-MB-231 using reverse transfection. Furthermore, we describe the setup for two separate methods for detecting viable and dead cells using either homogenous assays or image-based analysis.
authors: Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Tukholmankatu 8, Helsinki, FI-00290, Finland. carina.vonschantz-fant@helsinki.fi.
authors: Carina
authors: C
authors: von Schantz
authors: Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Tukholmankatu 8, Helsinki, FI-00290, Finland.
authors: Jani
authors: J
authors: Saarela
mesh_terms: D045744:Cell Line, Tumor; D005260:Female; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
chemical_list: D034741:RNA, Small Interfering
keywords: Acoustic droplet ejection; Gene silencing; High throughput screening; RNAi; Reverse transfection; siRNA screening