title: An acid precipitation technique: A strip assay for the large-scale DNA polymerase activity screening.
abstract: A new format of a very rapid, low-cost and high-productive analysis based on the acid precipitation of radiolabeled DNA was developed. By contrast to the conventional processing of a large number of GF/C discs, the method employs one GF/C strip containing samples on individual teeth. The strip assay was validated by comparison with the glass fiber disk technique; the efficiency was demonstrated by screening E. coli superproducers and fractions obtained at the steps of Bst DNA polymerase, Large Fragment purification by the protocol we developed. The principle proposed allows simultaneous assaying many samples for the activity of different polymerases.
authors: Institute of Molecular Genetics, Russian Academy of Sciences, 123182 Moscow, Russian Federation.
authors: Maria Yu
authors: MY
authors: Kordyukova
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation.
authors: Valeriya N
authors: VN
authors: Antipova
authors: Institute of Cell Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation.
authors: Vadim V
authors: VV
authors: Rogachevsky
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation. Electronic address: zyrina.nv@gmail.com.
authors: Nadezhda V
authors: NV
authors: Zyrina
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D001411:Geobacillus stearothermophilus / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase
keywords: Bst DNA polymerase; DNA polymerase activity screening; GF/C glass fiber; Large fragment purification