title: Radiotherapy changes salivary properties and impacts quality of life of children with Hodgkin disease.
abstract: OBJECTIVE
  We aimed to perform a longitudinal investigation of the effects of radiotherapy on salivary flow rate, pH, buffering capacity, and protein composition of saliva and on the quality of life of children with Hodgkin disease.
  
  
  METHODS
  Ten children (6-16-year-old) with Hodgkin disease and 10 matched healthy children were investigated. Stimulated and non-stimulated saliva samples were collected at baseline, after 1080 and 2160cGy of radiation, and 1, 2, and 3 months post-radiotherapy. The salivary flow rate was expressed as mL/min. Buffer capacity was determined by titration. Amylase activity, immunoglobulin A, mucin, and lactoferrin concentrations were determined by ELISA. Quality of life was assessed by Quality of Life - Head and Neck module 35 questionnaire.
  
  
  RESULTS
  We found that radiotherapy caused hyposalivation at 1080cGy and 1 month after radiotherapy and reduced buffering capacity at 2160cGy. Mucin concentration and amylase activity in non-stimulated saliva increased 1 month after radiotherapy. Lactoferrin concentration increased during and after radiotherapy. Immunoglobulin A concentration increased at 1080cGy, 1 and 2 months, for non-stimulated saliva and at 2160cGy and 1 month for stimulated saliva. Children reported more pain after radiotherapy and more xerostomia during radiotherapy.
  
  
  CONCLUSIONS
  We concluded that the radiotherapy protocol affected the children's salivary properties and children's quality of life.
authors: Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil Avenida Limeira 901, Piracicaba, SP, 13414-900, Brazil. Electronic address: lenita_m_l@hotmail.com.
authors: L
authors: L
authors: Marangoni-Lopes
authors: Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil Avenida Limeira 901, Piracicaba, SP, 13414-900, Brazil. Electronic address: livia_pagotto@hotmail.com.
authors: L P
authors: LP
authors: Rodrigues
authors: Boldrini Children's Center, Campinas, SP, Brazil Rua Dr. Gabriel Porto, 1270, Campinas, SP, 13083-210, Brazil. Electronic address: reginamhm@gmail.com.
authors: R H
authors: RH
authors: Mendonça
authors: Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil Avenida Limeira 901, Piracicaba, SP, 13414-900, Brazil. Electronic address: nobre@fop.unicamp.br.
authors: M
authors: M
authors: Nobre-Dos Santos
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006689:Hodgkin Disease / Q000532:radiotherapy*; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007781:Lactoferrin / Q000378:metabolism; D008297:Male; D009077:Mucins / Q000378:metabolism; D011788:Quality of Life; D012463:Saliva / Q000737:chemistry*; D012472:Salivation / Q000528:radiation effects; D014987:Xerostomia / Q000209:etiology*
chemical_list: D007070:Immunoglobulin A; D009077:Mucins; D007781:Lactoferrin
keywords: Hodgkin disease; Quality-of-life; Radiotherapy; Saliva; Xerostomia