title: Purification and determination of C-reactive protein and inter-α-trypsin inhibitor heavy chain 4 in dogs after major surgery through generation of specific antibodies.
abstract: Inter-α-trypsin inhibitor heavy chain 4 (ITIH4) and C-reactive protein (CRP) have been isolated from acute phase dog sera by affinity chromatography with insolubilized polyclonal antibodies anti pig Major Acute phase Protein (Pig-MAP) and with p-Aminophenyl Phosphoryl Choline, respectively. Isolated proteins were used to prepare specific polyclonal rabbit antisera that have allowed quantifying their concentration in serum samples by single radial immunodifussion. Both proteins were quantified in sera from female dogs that had undergone ovariohysterectomy (OVH, n=9) or mastectomy (n=10). The observed increases in CRP concentrations showed that surgical traumas induced an acute phase response of a great magnitude in the dogs. In both surgeries a four-fold increase of ITIH4 concentrations was detected. It can be concluded that ITIH4 is a new positive acute phase protein in dogs, as reported in other species.
authors: Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain; Acuvet Biotech, Bari 25, duplicado, 50197, Zaragoza, Spain.
authors: L
authors: L
authors: Soler
authors: Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain.
authors: N
authors: N
authors: García
authors: Hospital Veterinario, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
authors: A
authors: A
authors: Unzueta
authors: Acuvet Biotech, Bari 25, duplicado, 50197, Zaragoza, Spain.
authors: M
authors: M
authors: Piñeiro
authors: Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain.
authors: M A
authors: MA
authors: Álava
authors: Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain. Electronic address: lampreav@unizar.es.
authors: F
authors: F
authors: Lampreave
mesh_terms: D000510:Alpha-Globulins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002097:C-Reactive Protein / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D004285:Dogs; D005260:Female; D007106:Immune Sera / Q000276:immunology; D011817:Rabbits
chemical_list: D000510:Alpha-Globulins; D000906:Antibodies; D007106:Immune Sera; C011877:inter-alpha-inhibitor; D002097:C-Reactive Protein
keywords: Acute phase proteins; CRP; Dog/Canine; ITIH4; Surgical trauma