title: Metabolomics Analyses of Cancer Cells in Controlled Microenvironments.
abstract: The tumor microenvironment is a complex and heterogeneous milieu in which cancer cells undergo metabolic reprogramming to fuel their growth. Cancer cell lines grown in vitro using traditional culture methods represent key experimental models to gain a mechanistic understanding of tumor biology. This protocol describes the use of gas chromatography-mass spectrometry (GC-MS) to assess metabolic changes in cancer cells grown under varied levels of oxygen and nutrients that may better mimic the tumor microenvironment. Intracellular metabolite changes, metabolite uptake and release, as well as stable isotope ((13)C) tracer analyses are done in a single experimental setup to provide an integrated understanding of metabolic adaptation. Overall, this chapter describes some essential tools and methods to perform comprehensive metabolomics analyses.
authors: Department of Biochemistry, McGill University, Montréal, QC, Canada, H3G 1Y6.|Goodman Cancer Research Centre, McGill University, Montréal, QC, Canada, H3A 1A3.
authors: Simon-Pierre
authors: SP
authors: Gravel
authors: Goodman Cancer Research Centre, McGill University, Montréal, QC, Canada, H3A 1A3.|Metabolomics Core Facility, Goodman Cancer Research Centre, McGill University, Montréal, QC, Canada.
authors: Daina
authors: D
authors: Avizonis
authors: Department of Biochemistry, McGill University, Montréal, QC, Canada, H3G 1Y6. julie.st-pierre@mcgill.ca.|Goodman Cancer Research Centre, McGill University, Montréal, QC, Canada, H3A 1A3. julie.st-pierre@mcgill.ca.
authors: Julie
authors: J
authors: St-Pierre
mesh_terms: D045744:Cell Line, Tumor; D002478:Cells, Cultured; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D000860:Hypoxia / Q000378:metabolism; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D059016:Tumor Microenvironment; D057188:Workflow
keywords: GC-MS; Hypoxia; Metabolomics; Methoximation; Microenvironment; Nutrients; Silylation; Stable isotope