title: The Role of Notch1 Signaling in Anaplastic Thyroid Carcinoma.
abstract: OBJECTIVE
  The Notch signaling pathway is widely expressed in normal, reactive, and neoplastic tissues; however, its role in thyroid tissues has not been fully elucidated. Therefore, this study was conducted to characterize the expression of the Notch signaling pathway in papillary thyroid cancer (PTC) cells and anaplastic thyroid cancer (ATC) cells.
  
  
  METHODS
  Expression of activated Notch1 in ATC and PTC paraffin-embedded tissues was determined by immunohistochemistry. The small interfering RNA techniquewas employed to knock down Notch1 expression in ATC and PTC cell lines.
  
  
  RESULTS
  The expression of activated Notch1 was higher in ATC cases than in PTC cases. Inhibition of Notch1 significantly reduced proliferation and migration of ATC cells, but not PTC cells. In addition, inhibition of Notch1 in ATC cells significantly reduced the expression of key markers of epithelial-mesenchymal transition and cancer stem cells. Conversely, changes in the expression of these proteins were not observed in PTC cells.
  
  
  CONCLUSIONS
  The results of this study suggest that Notch1 expression plays different roles in tumor progression in ATC and PTC cells. We also found that Notch1 expression was significantly related to the highly invasive or proliferative activity of ATC cells.
authors: Laboratory of Radiation Pathology, Korea Cancer Center Hospital, Seoul, Korea.
authors: Hyeon Jin
authors: HJ
authors: Kim
authors: Laboratory of Radiation Pathology, Korea Cancer Center Hospital, Seoul, Korea.
authors: Min-Jung
authors: MJ
authors: Kim
authors: Laboratory of Radiation Pathology, Korea Cancer Center Hospital, Seoul, Korea.
authors: Areumnuri
authors: A
authors: Kim
authors: Department of Pathology, Korea Cancer Center Hospital, Seoul, Korea.
authors: Chang Won
authors: CW
authors: Jung
authors: Laboratory of Radiation Pathology, Korea Cancer Center Hospital, Seoul, Korea.|Department of Pathology, Korea Cancer Center Hospital, Seoul, Korea.
authors: Sunhoo
authors: S
authors: Park
authors: Department of Pathology, Korea Cancer Center Hospital, Seoul, Korea.
authors: Jae Soo
authors: JS
authors: Koh
authors: Laboratory of Radiation Pathology, Korea Cancer Center Hospital, Seoul, Korea.|Department of Pathology, Korea Cancer Center Hospital, Seoul, Korea.
authors: Jae Kyung
authors: JK
authors: Myung
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D034741:RNA, Small Interfering / Q000235:genetics; D051881:Receptor, Notch1 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D065646:Thyroid Carcinoma, Anaplastic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D004791:Enzyme Inhibitors; D034741:RNA, Small Interfering; D051881:Receptor, Notch1; D053829:Amyloid Precursor Protein Secretases
keywords: Anaplastic thyroid carcinoma; Epithelial-mesenchymal transition; Neoplastic stem cells; Papillary thyroid cancer; Receptor Notch1