title: Altered tooth morphogenesis after silencing the planar cell polarity core component, Vangl2.
abstract: Vangl2, one of the core components of the planar cell polarity (PCP) pathway, has an important role in the regulation of morphogenesis in several tissues. Although the expression of Vangl2 has been detected in the developing tooth, its role in tooth morphogenesis is not known. In this study, we show that Vangl2 is expressed in the inner dental epithelium (IDE) and in the secondary enamel knots (SEKs) of bell stage tooth germs. Inhibition of Vangl2 expression by siRNA treatment in in vitro-cultured tooth germs resulted in retarded tooth germ growth with deregulated cell proliferation and apoptosis. After kidney transplantation of Vangl2 siRNA-treated tooth germs, teeth were observed to be small and malformed. We also show that Vangl2 is required to maintain the proper pattern of cell alignment in SEKs, which maybe important for the function of SEKs as signaling centers. These results suggest that Vangl2 plays an important role in the morphogenesis of teeth.
authors: Applied Oral Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong, SAR, People's Republic of China.
authors: Zhaoming
authors: Z
authors: Wu
authors: Applied Oral Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong, SAR, People's Republic of China.
authors: Don Jeevanie
authors: DJ
authors: Epasinghe
authors: Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangzhou Medical College, Guangzhou, Guangdong Province, People's Republic of China.
authors: Jinquan
authors: J
authors: He
authors: Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea.
authors: Liwen
authors: L
authors: Li
authors: Applied Oral Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong, SAR, People's Republic of China.
authors: David W
authors: DW
authors: Green
authors: Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea.
authors: Min-Jung
authors: MJ
authors: Lee
authors: Applied Oral Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong, SAR, People's Republic of China. hsj8076@gmail.com.|Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea. hsj8076@gmail.com.
authors: Han-Sung
authors: HS
authors: Jung
mesh_terms: D000818:Animals; D017209:Apoptosis; D016764:Cell Polarity; D049109:Cell Proliferation; D020868:Gene Silencing; D008813:Mice, Inbred ICR; D009024:Morphogenesis; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D014070:Tooth / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D014083:Tooth Germ / Q000166:cytology / Q000378:metabolism
chemical_list: C431577:Ltap protein, mouse; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering
keywords: Enamel knot; Morphogenesis; Planar cell polarity; Tooth; Vangl2