title: Design of starch functionalized biodegradable P(MAA-co-MMA) as carrier matrix for l-asparaginase immobilization.
abstract: We prepared biodegradable P(MAA-co-MMA)-starch composite as carrier matrix for the immobilization of l-asparaginase (l-ASNase), an important chemotherapeutic agent in acute lymphoblastic leukemia. Chemical characteristics and thermal stability of the prepared composites were determined by FT-IR, TGA, DTA and, DSC, respectively. Also, biodegradability measurements of P(MAA-co-MMA)-starch composites were carried out to examine the effects of degradation of the starch. Then, l-ASNase was immobilized on the P(MAA-co-MMA)-starch composites. The surface morphology of the composite before and after immobilization was characterized by SEM, EDX, and AFM. The properties of the immobilized l-ASNase were investigated and compared with the free enzyme. The immobilized l-ASNase had better showed thermal and pH stability, and remained stable after 30days of storage at 25°C. Thus, based on the findings of the present work, the P(MAA-co-MMA)-starch composite can be exploited as the biocompatible matrix used for l-ASNase immobilization for medical applications due to biocompatibility and biodegradability.
authors: Department of Chemistry, Science Faculty, Inonu University, 44280, Malatya, Turkey.
authors: Ahmet
authors: A
authors: Ulu
authors: Department of Chemistry, Science Faculty, Inonu University, 44280, Malatya, Turkey.
authors: Suleyman
authors: S
authors: Koytepe
authors: Department of Chemistry, Science Faculty, Inonu University, 44280, Malatya, Turkey. Electronic address: burhan.ates@inonu.edu.tr.
authors: Burhan
authors: B
authors: Ates
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D001215:Asparaginase / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D002460:Cell Line; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006801:Humans; D008768:Methylmethacrylates / Q000737:chemistry; D051379:Mice; D011109:Polymethacrylic Acids / Q000737:chemistry; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy; D013213:Starch / Q000031:analogs & derivatives*
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; D008768:Methylmethacrylates; D011109:Polymethacrylic Acids; D013213:Starch; D001215:Asparaginase
keywords: Biocomposite; Enzyme immobilization; P(MAA-co-MMA); Starch; l-Asparaginase