title: Effect of Nitric Oxide on the Antifungal Activity of Oxidative Stress and Azoles Against Candida albicans.
abstract: Nitric oxide (NO) is a small molecule with a wide range of biological activities in mammalian and bacteria. However, the role of NO in fungi, especially Candida albicans, is not clear. In this study, we confirmed the generation of endogenous NO in C. albicans, and found that the production of endogenous NO in C. albicans was associated with nitric oxide synthase pathway. Our results further indicated that the production of endogenous NO in C. albicans was reduced under oxidative stress such as menadione or H2O2 treatment. Meanwhile, exogenous NO donor, sodium nitroprusside (SNP), synergized with H2O2 against C. albicans. Interestingly, SNP could inhibit the antifungal effect of azoles against C. albicans in vitro, suggesting that NO might be involved in the resistance of C. albicans to antifungals. Collectively, this study demonstrated the production of endogenous NO in C. albicans, and indicated that NO may play an important role in the response of C. albicans to oxidative stress and azoles.
authors: Department of Clinical Pharmacology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853 China.|New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, 200433 China.
authors: De-Dong
authors: DD
authors: Li
authors: The First Department of South Building, General Hospital of Chinese Armed Police Forces, Beijing, 100039 China.
authors: Chang-Chun
authors: CC
authors: Yang
authors: Department of Clinical Pharmacology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853 China.
authors: Ping
authors: P
authors: Liu
authors: New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, 200433 China.
authors: Yan
authors: Y
authors: Wang
authors: Department of Clinical Pharmacology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853 China.
authors: Yan
authors: Y
authors: Sun
keywords: Azoles; Candida albicans; Nitric oxide; Oxidative stress