title: Expression patterns of T-type Cav3.2 channel and insulin-like growth factor-1 receptor in dorsal root ganglion neurons of mice after sciatic nerve axotomy.
abstract: Substantial evidence indicates that T-type Cav3.2 channel and insulin-like growth factor-1 (IGF-1) contribute to pain hypersensitivity within primary sensory nerves. A recent study suggested that activation of IGF-1 receptor (IGF-1R) could increase Cav3.2 channel currents and further contribute to inflammatory pain sensitivity. However, the expression patterns of Cav3.2 and IGF-1R and their colocalization in dorsal root ganglion (DRG) in chronic neuropathic pain condition remain unknown. In this study, we explored expression patterns of Cav3.2, IGF-1R and their colocalization, and whether phenotypic switch occurs in a subpopulation of Cav3.2 or IGF-1R neurons in mouse DRGs after sciatic nerve axotomy with immunofluorescence, real-time reverse transcription-PCR, and western blot assays. We found that expressions of Cav3.2 and IGF-1R, and their colocalization were not increased in DRGs of mice following axotomy. In addition, Cav3.2 or IGF-1R subpopulation neurons did not acquire significant switch in expression phenotype after sciatic nerve axotomy. Our findings argue for an upregulation of Cav3.2 and IGF-1R expression in lumbar DRGs post-sciatic nerve axotomy and provided an insight for understanding the functions of peripheral afferent Cav3.2 channel and IGF-1/IGF-1R signaling in chronic neuropathic pain.
authors: aDepartment of Anesthesiology, China-Japan Friendship Hospital, Beijing bJiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu cState Key Laboratory of Neuroscience, Institute of Neuroscience, Shanghai Institutes for Biological Sciences, CAS Center for Excellence in Brain Science & Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.
authors: Si-Fang
authors: SF
authors: Lin
authors: Xiao-Lu
authors: XL
authors: Yu
authors: Xiao-Ya
authors: XY
authors: Liu
authors: Bing
authors: B
authors: Wang
authors: Cheng-Hui
authors: CH
authors: Li
authors: Yan-Gang
authors: YG
authors: Sun
authors: Xing-Jun
authors: XJ
authors: Liu
mesh_terms: D000818:Animals; D019771:Axotomy / Q000009:adverse effects; D020747:Calcium Channels, T-Type / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000473:pathology*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger; D020426:Sciatic Neuropathy / Q000473:pathology*
chemical_list: C504883:Cacna1h protein, mouse; D020747:Calcium Channels, T-Type; D012333:RNA, Messenger; C519530:insulin-like growth factor-1, mouse; D007334:Insulin-Like Growth Factor I