title: The effects of ethanol on angiogenesis after myocardial infarction, and preservation of angiogenesis with rosuvastatin after heavy drinking.
abstract: The cardioprotective effects of moderate alcohol consumption and statins have been known for years. However, heavy or binge drinking confers a high risk of cardiovascular disease. This study aimed to investigate the effects of different levels of alcohol consumption on acute myocardial infarction that was induced experimentally in rats, with a focus on the potential mechanism of angiogenesis and the effects of statins on heavy drinking. The experimental rats were fed low-dose ethanol (0.5 g/kg/day), high-dose ethanol (5 g/kg/day), and high-dose ethanol with rosuvastatin (10 mg/kg/day) during the entire experiment. Acute myocardial infarctions were induced 4 weeks after the beginning of the experiment. We assessed the capillary density in the myocardium via immunohistochemistry and quantified the expression of vascular endothelial growth factor (VEGF) and endostatin via enzyme-linked immunosorbent assay kits on the 4th day after myocardial infarction. The results revealed that low ethanol consumption promoted angiogenesis in association with higher VEGF and lower endostatin. High ethanol intake suppressed angiogenesis with unchanged VEGF and elevated endostatin. Treatment with rosuvastatin preserved angiogenesis following high ethanol intake, with an upregulation of VEGF. This study highlights that low ethanol consumption obviously promotes angiogenesis in myocardial-infarction rats while increasing the expression of VEGF, whereas high ethanol consumption inhibits ischemia-induced angiogenesis. This study also provides evidence that rosuvastatin alleviates the inhibitory effects of heavy drinking on angiogenesis.
authors: Department of Cardiology, Shandong Provincial Hospital, Shandong University, Jinan, China.
authors: Yuying
authors: Y
authors: Zhang
authors: Department of Cardiology, Shandong Provincial Hospital, Shandong University, Jinan, China.
authors: Haitao
authors: H
authors: Yuan
authors: Department of Cardiology, Shandong Provincial Hospital, Shandong University, Jinan, China.
authors: Yongle
authors: Y
authors: Sun
authors: Department of Cardiology, Shandong Provincial Hospital, Shandong University, Jinan, China.
authors: Yong
authors: Y
authors: Wang
authors: Department of Cardiology, Shandong Provincial Hospital, Shandong University, Jinan, China. Electronic address: 15168886621@163.com.
authors: Aihong
authors: A
authors: Wang
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D043169:Endostatins / Q000378:metabolism; D000431:Ethanol / Q000008:administration & dosage* / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006321:Heart / Q000502:physiology; D008297:Male; D009203:Myocardial Infarction / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000068718:Rosuvastatin Calcium / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D002316:Cardiotonic Agents; D043169:Endostatins; D042461:Vascular Endothelial Growth Factor A; D000431:Ethanol; D000068718:Rosuvastatin Calcium
keywords: Angiogenesis; Endostatin; Ethanol; Myocardial infarction; Rosuvastatin; VEGF