title: Validation approach for a fast and simple targeted screening method for 75 antibiotics in meat and aquaculture products using LC-MS/MS.
abstract: An approach is described to validate a fast and simple targeted screening method for antibiotic analysis in meat and aquaculture products by LC-MS/MS. The strategy of validation was applied for a panel of 75 antibiotics belonging to different families, i.e., penicillins, cephalosporins, sulfonamides, macrolides, quinolones and phenicols. The samples were extracted once with acetonitrile, concentrated by evaporation and injected into the LC-MS/MS system. The approach chosen for the validation was based on the Community Reference Laboratory (CRL) guidelines for the validation of screening qualitative methods. The aim of the validation was to prove sufficient sensitivity of the method to detect all the targeted antibiotics at the level of interest, generally the maximum residue limit (MRL). A robustness study was also performed to test the influence of different factors. The validation showed that the method is valid to detect and identify 73 antibiotics of the 75 antibiotics studied in meat and aquaculture products at the validation levels.
authors: a Residues and Contaminants Unit, Fougeres Laboratory , ANSES (National Agency for Food, Environment and Occupational Health & Safety) , Fougeres Cedex , France.
authors: Estelle
authors: E
authors: Dubreil
authors: a Residues and Contaminants Unit, Fougeres Laboratory , ANSES (National Agency for Food, Environment and Occupational Health & Safety) , Fougeres Cedex , France.
authors: Sophie
authors: S
authors: Gautier
authors: a Residues and Contaminants Unit, Fougeres Laboratory , ANSES (National Agency for Food, Environment and Occupational Health & Safety) , Fougeres Cedex , France.
authors: Marie-Pierre
authors: MP
authors: Fourmond
authors: a Residues and Contaminants Unit, Fougeres Laboratory , ANSES (National Agency for Food, Environment and Occupational Health & Safety) , Fougeres Cedex , France.
authors: Mélaine
authors: M
authors: Bessiral
authors: a Residues and Contaminants Unit, Fougeres Laboratory , ANSES (National Agency for Food, Environment and Occupational Health & Safety) , Fougeres Cedex , France.
authors: Murielle
authors: M
authors: Gaugain
authors: b European Union Reference Laboratory for Antibiotic and Dye Residue in Food, Fougeres Laboratory , ANSES (National Agency for Food, Environment and Occupational Health & Safety) , Fougeres Cedex , France.
authors: Eric
authors: E
authors: Verdon
authors: a Residues and Contaminants Unit, Fougeres Laboratory , ANSES (National Agency for Food, Environment and Occupational Health & Safety) , Fougeres Cedex , France.
authors: Dominique
authors: D
authors: Pessel
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D017756:Aquaculture; D002417:Cattle; D002511:Cephalosporins / Q000032:analysis; D002853:Chromatography, Liquid / Q000592:standards*; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D017408:Guidelines as Topic; D006801:Humans; D018942:Macrolides / Q000032:analysis; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D010406:Penicillins / Q000032:analysis; D011200:Poultry; D015363:Quinolones / Q000032:analysis; D012474:Salmon; D012680:Sensitivity and Specificity; D012756:Sheep; D013449:Sulfonamides / Q000032:analysis; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000592:standards*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D018942:Macrolides; D010406:Penicillins; D015363:Quinolones; D013449:Sulfonamides
keywords: Antibiotics; aquaculture products; liquid chromatography/mass spectrometry (LC-MS/MS); meat; qualitative method; screening