title: Scanning electron microscopy image representativeness: morphological data on nanoparticles.
abstract: A sample of a nanomaterial contains a distribution of nanoparticles of various shapes and/or sizes. A scanning electron microscopy image of such a sample often captures only a fragment of the morphological variety present in the sample. In order to quantitatively analyse the sample using scanning electron microscope digital images, and, in particular, to derive numerical representations of the sample morphology, image content has to be assessed. In this work, we present a framework for extracting morphological information contained in scanning electron microscopy images using computer vision algorithms, and for converting them into numerical particle descriptors. We explore the concept of image representativeness and provide a set of protocols for selecting optimal scanning electron microscopy images as well as determining the smallest representative image set for each of the morphological features. We demonstrate the practical aspects of our methodology by investigating tricalcium phosphate, Ca3(PO4)2, and calcium hydroxyphosphate, Ca5(PO4)3(OH), both naturally occurring minerals with a wide range of biomedical applications.
authors: Laboratory of Environmental Chemometrics, Faculty of Chemistry, University of Gdansk, Gdansk, Poland.|Computational Research Division, Lawrence Berkeley National Laboratory, Berkeley, California, U.S.A.
authors: Katarzyna
authors: K
authors: Odziomek
authors: Computational Research Division, Lawrence Berkeley National Laboratory, Berkeley, California, U.S.A.
authors: Daniela
authors: D
authors: Ushizima
authors: Materials Design Division, Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, Poland.
authors: Przemyslaw
authors: P
authors: Oberbek
authors: Materials Design Division, Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, Poland.
authors: Krzysztof Jan
authors: KJ
authors: Kurzydłowski
authors: Laboratory of Environmental Chemometrics, Faculty of Chemistry, University of Gdansk, Gdansk, Poland.
authors: Tomasz
authors: T
authors: Puzyn
authors: Computational Research Division, Lawrence Berkeley National Laboratory, Berkeley, California, U.S.A.
authors: Maciej
authors: M
authors: Haranczyk
keywords: Ceramics; nanoparticles; representativeness