title: Selection of strains for shiitake production in axenic substrate.
abstract: Shiitake mushroom consumption is increasing in Brazil. In addition to the implementation of new production methods, it is also important to increase productivity, quality and reduce production costs. In this study, six commercial Lentinula edodes strains were characterized for genetic diversity (rep-PCR analysis) and mushroom production (yield, number and weight of individual mushrooms) using different substrates and cultural conditions. All strains showed genetic differences by repetitive element palindromic based-polymerase chain reaction (rep-PCR). The richest substrate resulted in the greatest production under both environmental conditions. Strains LE4 and LE6 produced the majority of their mushrooms earlier than the other strains. The highest number of mushrooms was observed in the LE6 strain while the highest weights of individual mushrooms were observed in the LE4 strain. Controlled environmental conditions resulted in superior production for all strains, except for LE4, which had empirically greater yield in the semi-controlled environmental condition.
authors: Faculdade de Ciências Agrárias e Tecnológicas (FCAT), Universidade Estadual Paulista (UNESP), Dracena, SP, Brazil. dczied@gmail.com.
authors: Diego Cunha
authors: DC
authors: Zied
authors: Departamento de Biologia, Universidade Federal de Lavras (UFLA), PO Box 3037, Lavras, MG, 37200-000, Brazil.
authors: William Pereira
authors: WP
authors: Maciel
authors: Departamento de Biologia, Universidade Federal de Lavras (UFLA), PO Box 3037, Lavras, MG, 37200-000, Brazil.
authors: Simone Cristina
authors: SC
authors: Marques
authors: Departamento de Biologia, Universidade Federal de Lavras (UFLA), PO Box 3037, Lavras, MG, 37200-000, Brazil.
authors: Débora Marques
authors: DM
authors: da Silveira E Santos
authors: Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada.
authors: Danny Lee
authors: DL
authors: Rinker
authors: Departamento de Biologia, Universidade Federal de Lavras (UFLA), PO Box 3037, Lavras, MG, 37200-000, Brazil.
authors: Eustáquio Souza
authors: ES
authors: Dias
mesh_terms: D000383:Agriculture / Q000379:methods; D061245:Axenic Culture / Q000379:methods*; D001938:Brazil; D004271:DNA, Fungal / Q000032:analysis; D014644:Genetic Variation; D016133:Polymerase Chain Reaction / Q000379:methods; D040641:Quantitative Trait Loci; D020942:Shiitake Mushrooms / Q000145:classification / Q000235:genetics* / Q000254:growth & development*
chemical_list: D004271:DNA, Fungal
keywords: Genetic diversity; Lentinula edodes; Mushroom yield; Strain selection