title: Degradation kinetics and structural characteristics of pectin under simultaneous sonochemical-enzymatic functions.
abstract: This study investigated the degradation kinetics and structural properties of pectin with combining ultrasound and pectinase treatment. Ultrasound at an intensity of 4.5WmL(-1) and a time of 10min significantly enhanced the enzymatic degradation of pectin weight-average molecular weight (Mw). The degradation kinetics model of pectin followed 1/Mwt-1/Mw0=kt, suggesting the randomness of the degradation process. Synergistic effects of ultrasound and pectinase were observed at 20-60°C and were more effective at lower temperatures. Furthermore, the degree of methoxylation (DM) of sonoenzymolysis pectin significantly decreased whereas the degree of acetylation (DAc) remained unchanged compared to the original and enzymolysis pectin. Simultaneous functions of ultrasound and pectinase caused severe decomposition in pectin homogalacturonan (HG) regions without altering the monosaccharides types, configurations and glycoside linkages of the pectin samples. The complex polymeric structures of pectin transformed into smaller units with simpler branches and shorter chains after sonoenzymolysis reactions.
authors: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors: Xiaobin
authors: X
authors: Ma
authors: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors: Wenjun
authors: W
authors: Wang
authors: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors: Danli
authors: D
authors: Wang
authors: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R&D Center for Food Technology and Equipment, Hangzhou 310058, China.
authors: Tian
authors: T
authors: Ding
authors: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R&D Center for Food Technology and Equipment, Hangzhou 310058, China.
authors: Xingqian
authors: X
authors: Ye
authors: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R&D Center for Food Technology and Equipment, Hangzhou 310058, China. Electronic address: dhliu@zju.edu.cn.
authors: Donghong
authors: D
authors: Liu
mesh_terms: D000107:Acetylation; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D049329:Nanostructures / Q000737:chemistry; D010368:Pectins / Q000737:chemistry* / Q000378:metabolism; D011096:Polygalacturonase / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D014465:Ultrasonics / Q000379:methods
chemical_list: D009005:Monosaccharides; D010368:Pectins; D011096:Polygalacturonase; C003181:polygalacturonic acid
keywords: Demethoxylation; Kinetics model; Molecular weight; Nanostructure; Primary structure; Synergistic effect