title: Synthesis of curcumin-loaded chitosan phosphate nanoparticle and study of its cytotoxicity and antimicrobial activity.
abstract: Curcumin has acquired an important position in the treatment of various diseases. But its use, as a chemotherapeutic agent, is limited due to its low water solubility, poor bioavailability, and its sensitive nature at the physiological pH. To overcome this, curcumin was loaded into chitosan phosphate nanoparticles (CPNs). The loading efficiency was found to be 84%. DLS studies revealed the average particle size of CPNs and curcumin-loaded CPNs as 53 and 91 nm, respectively, and TEM results supplemented these values. A sustained release pattern was noticed and the amount of curcumin released in acidic pH was higher than at physiological pH. The curcumin nanoformulation exhibited proficient activity against both Gram-positive and Gram-negative bacteria as well as fungus. Cytocompatibility of the nanoformulations against peripheral blood mononuclear cells (PBMCs) and murine monocyte-macrophage cell line was confirmed by incubating with PBMCs and murine monocyte-macrophage cell line.
authors: a Department of Chemistry , Gauhati University , Guwahati , India.
authors: C
authors: C
authors: Deka
authors: b Department of Molecular Biology and Biotechnology , Tezpur University , Tezpur , India.
authors: L
authors: L
authors: Aidew
authors: a Department of Chemistry , Gauhati University , Guwahati , India.
authors: N
authors: N
authors: Devi
authors: b Department of Molecular Biology and Biotechnology , Tezpur University , Tezpur , India.
authors: A K
authors: AK
authors: Buragohain
authors: a Department of Chemistry , Gauhati University , Guwahati , India.
authors: D K
authors: DK
authors: Kakati
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D060326:Chemistry Techniques, Synthetic; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D003474:Curcumin / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D004339:Drug Compounding; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D000067996:RAW 264.7 Cells; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D004337:Drug Carriers; D014867:Water; D048271:Chitosan; D003474:Curcumin
keywords: Candida albicans; Curcumin; Escherichia coli; Mycobacterium smegmatis; Staphylococcu aureus; chitosan nanoparticles