title: Ficolin-2 binds to HIV-1 gp120 and blocks viral infection.
abstract: Ficolin-2 is a lectin complement pathway activator present in normal human plasma and usually associated with infectious diseases, but little is known about the role of ficolin-2 in human immunodeficiency virus (HIV) infection. Here, we describe our novel findings that serum ficolin-2 concentrations of 103 HIV-1 patients were much higher compared to those of 57 healthy donors. In vitro analysis showed that HIV-1 infection could enhance ficolin-2 expression. We further demonstrated that recombinant ficolin-2 protein could bind with HIV-1 envelope glycoprotein gp120, and subsequently induce complement dependent cytotoxicity. Moreover, ficolin-2 could block the entry of HIV-1 into target cells (TZM-b1 and MT-2 cells) and infection in a ficolin-2 dosedependent manner. To our knowledge, this is the first report about the protective role of ficolin-2 against HIV-1 infection and our study suggests that ficolin-2 is an important human innate immune molecule against HIV.
authors: The State Key Laboratory of Virology, Department of Immunology and Hubei Province Key Laboratory of Allergy and Immune-related Diseases, Wuhan University School of Medicine, Wuhan, 430071, China.
authors: Fengling
authors: F
authors: Luo
authors: The State Key Laboratory of Virology, Department of Immunology and Hubei Province Key Laboratory of Allergy and Immune-related Diseases, Wuhan University School of Medicine, Wuhan, 430071, China.|Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors: Tielong
authors: T
authors: Chen
authors: The State Key Laboratory of Virology, Department of Immunology and Hubei Province Key Laboratory of Allergy and Immune-related Diseases, Wuhan University School of Medicine, Wuhan, 430071, China.
authors: Jun
authors: J
authors: Liu
authors: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
authors: Xihui
authors: X
authors: Shen
authors: The State Key Laboratory of Virology, Department of Immunology and Hubei Province Key Laboratory of Allergy and Immune-related Diseases, Wuhan University School of Medicine, Wuhan, 430071, China.
authors: Yinnan
authors: Y
authors: Zhao
authors: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. ryang@wh.iov.cn.
authors: Rongge
authors: R
authors: Yang
authors: The State Key Laboratory of Virology, Department of Immunology and Hubei Province Key Laboratory of Allergy and Immune-related Diseases, Wuhan University School of Medicine, Wuhan, 430071, China. zhangxiaolian@whu.edu.cn.
authors: Xiaolian
authors: X
authors: Zhang
mesh_terms: D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000378:metabolism*; D015658:HIV Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D037102:Lectins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D014779:Virus Replication; D000097604:Ficolins
chemical_list: D015699:HIV Envelope Protein gp120; D037102:Lectins; D000097604:Ficolins
keywords: HIV-1; ficolin-2; gp120