title: Metagenomic and Proteomic Analyses of a Mangrove Microbial Community Following Green MacroalgaeEnteromorpha proliferaDegradation.
abstract: A mangrove microbial community was analyzed at the gene and protein levels using metagenomic and proteomic methods with the green macroalgaeEnteromorpha proliferaas the substrate. Total DNA was sequenced on the Illumina HiSeq 2000 PE-100 platform. Two-dimensional gel electrophoresis in combination with liquid chromatography tandem mass spectrometry was used for proteomic analysis. The metagenomic data revealed that the orders Pseudomonadales, Rhizobiales, and Sphingomonadales were the most prevalent in the mangrove microbial community. By monitoring changes at the functional level, proteomic analyses detected ATP synthase and transporter proteins, which were expressed mainly by members of the phyla Proteobacteria and Bacteroidetes. Members of the phylum Proteobacteria expressed a high number of sugar transporters and demonstrated specialized and efficient digestion of various glycans. A few glycoside hydrolases were detected in members of the phylum Firmicutes, which appeared to be the main cellulose-degrading bacteria. This is the first report of multiple "omics" analysis ofE. proliferadegradation. These results support the fact that key enzymes of glycoside hydrolase family were expressed in large quantities, indicating the high metabolic activity of the community.
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China.
authors: Yijing
authors: Y
authors: Wu
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China.|Department of Chemistry, University of California, Davis, CA 95616, USA.
authors: Chao
authors: C
authors: Zhao
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China.
authors: Zheng
authors: Z
authors: Xiao
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China.
authors: Hetong
authors: H
authors: Lin
authors: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, P.R. China.
authors: Lingwei
authors: L
authors: Ruan
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China.|National Engineering Research Center of Juncao, Fuzhou 350002, P.R. China.
authors: Bin
authors: B
authors: Liu
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D044822:Biodiversity; D056186:Metagenomics; D040901:Proteomics; D012624:Seaweed / Q000378:metabolism / Q000382:microbiology*; D044445:Ulva / Q000378:metabolism / Q000382:microbiology*; D053833:Wetlands
chemical_list: D001426:Bacterial Proteins
keywords: Enteromorpha prolifera; degrading-community; mangrove; metagenomics; proteomics