title: Microbiological study on bioremediation of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) contaminated soil by agricultural waste composting.
abstract: This paper studied the degradation of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) in contaminated soil under composting and natural conditions, respectively. BDE-47 residue in agricultural waste-composting pile was determined during 45-day composting. The microbial communities were determined by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), and the relationships between the DGGE results and physico-chemical parameters were evaluated by redundancy analysis (RDA) and heatmap-clustering analysis. The results showed that the degradation rate of BDE-47 was significantly higher in agricultural waste-composting pile compared with control group, which was enhanced up to almost 15 % at the end of composting. There were different environmental factors which affected the distribution of composting bacterial and fungal communities. The bacterial community composition was more significantly affected by the addition of BDE-47 compared with other physico-chemical parameters, and BDE-47 had stronger influences on bacterial community than fungal community during the composting. Meanwhile, the most variation in distribution of fungal community was explained by pile temperature.
authors: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China. cyn@hnu.edu.cn.|Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, People's Republic of China. cyn@hnu.edu.cn.
authors: Yaoning
authors: Y
authors: Chen
authors: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.|Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, People's Republic of China.
authors: Shuang
authors: S
authors: Ma
authors: College of Municipal and Mapping Engineering, Hunan City University, Yiyang, Hunan, 413000, People's Republic of China. yuanpingl@163.net.
authors: Yuanping
authors: Y
authors: Li
authors: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.|Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, People's Republic of China.
authors: Ming
authors: M
authors: Yan
authors: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.|Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, People's Republic of China.
authors: Guangming
authors: G
authors: Zeng
authors: College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors: Jiachao
authors: J
authors: Zhang
authors: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.|Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, People's Republic of China.
authors: Jie
authors: J
authors: Zhang
authors: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.|Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, People's Republic of China.
authors: Xuebin
authors: X
authors: Tan
mesh_terms: D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics; D001673:Biodegradation, Environmental; D058448:Biota / Q000187:drug effects*; D058645:Denaturing Gradient Gel Electrophoresis; D005658:Fungi / Q000145:classification / Q000187:drug effects / Q000235:genetics; D055768:Halogenated Diphenyl Ethers / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013696:Temperature
chemical_list: D055768:Halogenated Diphenyl Ethers; D012987:Soil; D012989:Soil Pollutants; C511295:2,2',4,4'-tetrabromodiphenyl ether
keywords: BDE-47; Community composition; Composting; Contaminated soil; Degradation; PCR-DGGE