title: Molecular species delimitation methods and population genetics data reveal extensive lineage diversity and cryptic species in Aglaopheniidae (Hydrozoa).
abstract: A comprehensive inventory of global biodiversity would be greatly improved by automating methods for species delimitation. The Automatic Barcode Gap Discovery method, the Poisson tree processes algorithm and the Generalized mixed Yule-coalescent model have been proposed as means of increasing the rate of biodiversity description using single locus data. We applied these methods to explore the diversity within the Aglaopheniidae, a hydrozoan family with many species widely distributed across tropical and temperate oceans. Our analyses revealed widespread cryptic diversity in this family, almost half of the morpho-species presenting several independent evolutionary lineages, as well as support for cases of synonymy. For two common species of this family, Lytocarpia brevirostris and Macrorhynchia phoenicea, we compared the outputs to clustering analyses based on microsatellite data and to nuclear gene phylogenies. For L. brevirostris, microsatellite data were congruent with results of the species delimitation methods, revealing the existence of two cryptic species with Indo-Pacific distribution. For M. phoenicea, all analyses confirmed the presence of two cryptic species within the South-Western Indian Ocean. Our study suggests that the diversity of Aglaopheniidae might be much higher than assumed, likely related to low dispersal capacities. Sequence-based species delimitation methods seem highly valuable to reveal cryptic diversity in hydrozoans; their application in an integrative framework will be very useful in describing the phyletic diversity of these organisms.
authors: UMR ENTROPIE Université de La Réunion-CNRS-IRD, UFR Sciences et Technologies, 15 Avenue René Cassin, CS 92003, 97744 Saint Denis, France; Laboratoire d'Excellence CORAIL, France(1); IMBE UMR 7263, Aix Marseille Université-CNRS-IRD-Avignon Université, Station marine d'Endoume, Chemin de la batterie des lions, 13007 Marseille, France.. Electronic address: bautisse.postaire@imbe.fr.
authors: Bautisse
authors: B
authors: Postaire
authors: UMR ENTROPIE Université de La Réunion-CNRS-IRD, UFR Sciences et Technologies, 15 Avenue René Cassin, CS 92003, 97744 Saint Denis, France; Laboratoire d'Excellence CORAIL, France(1).
authors: Hélène
authors: H
authors: Magalon
authors: UMR ENTROPIE Université de La Réunion-CNRS-IRD, UFR Sciences et Technologies, 15 Avenue René Cassin, CS 92003, 97744 Saint Denis, France; Laboratoire d'Excellence CORAIL, France(1).
authors: Chloé A-F
authors: CA
authors: Bourmaud
authors: UMR ENTROPIE Université de La Réunion-CNRS-IRD, UFR Sciences et Technologies, 15 Avenue René Cassin, CS 92003, 97744 Saint Denis, France; Laboratoire d'Excellence CORAIL, France(1).
authors: J Henrich
authors: JH
authors: Bruggemann
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D037402:Hydrozoa / Q000145:classification* / Q000235:genetics*; D019156:Indian Ocean; D018895:Microsatellite Repeats / Q000235:genetics; D010802:Phylogeny; D013045:Species Specificity
keywords: 16S; Automatic Barcode Gap Discovery; Cnidarians; Generalized mixed Yule-coalescent model; Microsatellites; Poisson tree processes