title: Comparative microarray profile of the hepatopancreas in the response of "Huanghai No. 2" Fenneropenaeus chinensis to white spot syndrome virus.
abstract: White spot syndrome virus (WSSV) infects all shrimp species and is the greatest detriment to shrimp culture. To better understand the mechanism of molecular responses to WSSV infection in "Huanghai No. 2" Fenneropenaeus chinensis, a microarray technique was used. Microarray gene expression profiling of 59,137 unigenes identified Differentially Expressed Genes (DEGs) both in live and moribund shrimp at early, peak and late phases. In live shrimp, 1307, 1479 and 1539 DEGs were obtained in the early, peak and late phase, respectively. Meanwhile, 1536, 2181 and 1591 DEGs were obtained in moribund shrimp. Twenty known annotation genes are uniquely expressed in the late phase of live shrimp, including adhesion regulating molecule 1, arginine kinase, BUD31 homolog, and QM. Compared to WSSV-susceptible shrimp, 75 known annotation genes are uniquely expressed in WSSV-resistant shrimp, including arginine kinase, BUD31 homolog, clottable protein 2, caspase 2, cathepsin C, calnexin, HMGBb, Histone 3, and selenoprotein M. The gene expression patterns of the infected shrimp were altered by WSSV infection. To further confirm the expression of differentially expressed genes, real-time RT-PCR was performed to test six randomly selected genes. The data will provide valuable information to understand the immune mechanism of shrimp's response to WSSV.
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China.
authors: Xiaoli
authors: X
authors: Shi
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China. Electronic address: kongjie@ysfri.ac.cn.
authors: Jie
authors: J
authors: Kong
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China.
authors: Xianhong
authors: X
authors: Meng
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China.
authors: Sheng
authors: S
authors: Luan
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China.
authors: Kun
authors: K
authors: Luo
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China.
authors: Baoxiang
authors: B
authors: Cao
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China.
authors: Ning
authors: N
authors: Liu
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China.
authors: Xia
authors: X
authors: Lu
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China.
authors: Kangyu
authors: K
authors: Deng
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China.
authors: Jiawang
authors: J
authors: Cao
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China.
authors: Yingxue
authors: Y
authors: Zhang
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China.
authors: Hengheng
authors: H
authors: Zhang
authors: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 266071, PR China.
authors: Xupeng
authors: X
authors: Li
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D043143:Hepatopancreas / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology / Q000821:virology; D016133:Polymerase Chain Reaction; D059467:Transcriptome; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D060829:Arthropod Proteins
keywords: Crustacean immunity; Hepatopancreas; Microarray; White spot syndrome virus; “Huanghai No. 2” Fenneropenaeus chinensis