title: Shiga Toxin Subtypes and Virulence Genes in Escherichia coli Isolated from Cattle.
abstract: Subtypes of stx1 and stx2 in 45 Shiga toxin-producing Escherichia coli (STEC) strains isolated from cattle were investigated by PCR. Only subtype stx1a was detected among all the stx1-positive strains. The major stx2 subtype was stx2a followed by stx2d, stx2c, stx2b, and stx2g in decreasing order of frequency. stx2c was found in strains of serotypes O157 and O174. stx2d was found in 11 strains. These strains were confirmed by DNA sequencing to carry both the activatable tail and the END motif; all were eae-negative, and 3 contained stx2d as the only stx. stx2g was found in 2 strains in association with stx2a, estA1, and astA. In addition, 7 hybrid strains of shigatoxigenic and enterotoxigenic E. coli (STEC/ETEC) were found to harbor one or both of stx1a and stx2a (stx1a/stx2a) and estA1. Among 27 serotypes of STEC strains isolated from cattle, O157:H7 and O109:H- strains were eae-positive. Other putative adhesin genes, such as saa, iha, espP, and lpfAO113were detected in more than 12 serotypes.
authors: Public Health Science Research Center, Hyogo Prefectural Institute of Public Health and Consumer Sciences.
authors: Yumi
authors: Y
authors: Akiyama
authors: Hiroko
authors: H
authors: Futai
authors: Etsuko
authors: E
authors: Saito
authors: Kenichi
authors: K
authors: Ogita
authors: Hiroshi
authors: H
authors: Sakae
authors: Masaharu
authors: M
authors: Fukunaga
authors: Hidetaka
authors: H
authors: Tsuji
authors: Masatsugu
authors: M
authors: Chikahira
authors: Atsushi
authors: A
authors: Iguchi
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D005838:Genotype; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D022621:Shiga Toxin / Q000145:classification* / Q000235:genetics*; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D037521:Virulence Factors; D022621:Shiga Toxin