title: Quantification of Oxidized and Unsaturated Bile Alcohols in Sea Lamprey Tissues by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry.
abstract: A sensitive and reliable method was developed and validated for the determination of unsaturated bile alcohols in sea lamprey tissues using liquid-liquid extraction and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The liver, kidney, and intestine samples were extracted with acetonitrile and defatted by n-hexane. Gradient UHPLC separation was performed using an Acquity BEH C18 column with a mobile phase of water and methanol containing 20 mM triethylamine. Multiple reaction monitoring modes of precursor-product ion transitions for each analyte was used. This method displayed good linearity, with correlation coefficients greater than 0.99, and was validated. Precision and accuracy (RSD %) were in the range of 0.31%-5.28%, while mean recoveries were between 84.3%-96.3%. With this technique, sea lamprey tissue samples were analyzed for unsaturated bile alcohol analytes. This method is practical and particularly suitable for widespread putative pheromone residue analysis.
authors: Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. like4@msu.edu.
authors: Ke
authors: K
authors: Li
authors: Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. scottan7@msu.edu.
authors: Anne M
authors: AM
authors: Scott
authors: Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. chungyuw@msu.edu.
authors: Yu-Wen
authors: YW
authors: Chung-Davidson
authors: Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. bussy@msu.edu.
authors: Ugo
authors: U
authors: Bussy
authors: Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. pateltrinkal@gmail.com.
authors: Trinkal
authors: T
authors: Patel
authors: Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. middle73@msu.edu.
authors: Zoe E
authors: ZE
authors: Middleton
authors: Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA. liweim@msu.edu.
authors: Weiming
authors: W
authors: Li
mesh_terms: D000818:Animals; D002777:Cholestanols / Q000737:chemistry* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D007798:Lampreys / Q000378:metabolism*; D013058:Mass Spectrometry; D010084:Oxidation-Reduction
chemical_list: D002777:Cholestanols
keywords: UPLC-MS/MS; fish; metabolites; sulfated bile alcohol