title: Comparison of the Ovary and Kidney as Sites for Islet Transplantation in Diabetic Rats.
abstract: BACKGROUND
  Currently, the most commonly used site for clinical islet transplantation is the liver although it is far from being an ideal site. Low oxygen tension and the induction of an inflammatory response impair islet implantation and lead to significant early loss of islet. The present study aimed to investigate and compare the efficacy of islet transplantation to the ovary and kidney subcapsule in diabetic rats.
  
  
  METHODS
  The study was performed with 3 groups of rats (control, ovary, and kidney subcapsule) including 6 Sprague female rats each. Diabetes model was created with the use of streptozotocin, and blood glucose levels of the rats were measured after 72 hours. Thirty days after the transplantation, blood samples were obtained from the rats, and then pancreas, kidney, and ovary specimens were fixed in 10% formaldehyde and the experiment completed. After staining with hematoxylin and eosin, the tissue samples were morphologically evaluated by a specialist histopathologist.
  
  
  RESULTS
  Changes in mean blood glucose and C-peptide levels were statistically significant in the ovary and kidney subcapsule groups. Histologic examination revealed that granulosus insulin-bearing cells were detected in the islet grafts of both ovary and kidney subcapsule groups. The renal subcapsule group had inflammation signs on histologic examination. The islet cells of both ovary and renal subcapsule groups had no vacuolization.
  
  
  CONCLUSIONS
  We showed that the ovary might be a new site for islet transplantation. Further research should be done on whether the initial results of this study can be reproduced in larger numbers of animal models and eventually in humans.
authors: Department of Endocrinology and Metabolism, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey. Electronic address: meliakarakose@yahoo.com.
authors: M
authors: M
authors: Karakose
authors: Department of Genetic and Medical Research Center, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey.
authors: F A
authors: FA
authors: Pinarli
authors: Department of Endocrinology and Metabolism, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey.
authors: M S
authors: MS
authors: Arslan
authors: Adacell Laboratory, Pancreas Islet Cell Research Center, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey.
authors: G
authors: G
authors: Boyuk
authors: Adacell Laboratory, Pancreas Islet Cell Research Center, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey.
authors: B
authors: B
authors: Boztok
authors: Department of Pathology, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey.
authors: A
authors: A
authors: Albayrak
authors: Department of Cardiovascular Surgery, Hacettepe University School of Medicine, Ankara, Turkey.
authors: A T
authors: AT
authors: Ulus
authors: Department of Endocrinology and Metabolism, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey.
authors: E
authors: E
authors: Cakal
authors: Department of Internal Medicine, Kastamonu University School of Medicine, Kastamonu, Turkey.
authors: T
authors: T
authors: Delibasi
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002096:C-Peptide / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000601:surgery*; D005260:Female; D050417:Insulin-Secreting Cells / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D007668:Kidney / Q000166:cytology; D010053:Ovary / Q000166:cytology; D010179:Pancreas / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D002096:C-Peptide; D013311:Streptozocin