title: Antibacterial silk fibroin/nanohydroxyapatite hydrogels with silver and gold nanoparticles for bone regeneration.
abstract: The rapid emergence of antibiotic resistance is becoming an imminent problem in bone tissue engineering, and therefore biomaterials must be modified to promote the tissue integration before bacterial adhesion. In this work, silk fibroin/nanohydroxyapatite hydrogel was modified with in situ synthesized silver and gold nanoparticles (AgNPs and AuNPs), taking advantage of the tyrosine amino acid. The presence of AgNPs and AuNPs in the hydrogels was characterized by UV spectrophotometer, transmission electron microscopy and thermogravimetric analysis. In vitro antimicrobial studies revealed that hydrogels with AgNPs and AuNPs exhibited significant inhibition ability against both gram-positive and gram-negative bacteria. Cytocompatibility studies carried out using osteoblastic cells revealed that up to 0.5 wt% of AgNPs, and for all concentrations of AuNPs, the hydrogels can be effectively used as antimicrobial materials, without compromising cell behavior. On the basis of the aforementioned observations, these hydrogels are very attractive for bone tissue engineering.
authors: i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; FEUP - Faculdade de Engenharia da Universidade do Porto, Departamento de Engenharia Metalúrgica e Materiais, Porto, Portugal. Electronic address: marta.ribeiro@ineb.up.pt.
authors: Marta
authors: M
authors: Ribeiro
authors: INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; FP-ENAS/CEBIMED - University Fernando Pessoa Energy, Environment and Health Research Unit/Biomedical Research Center, Porto, Portugal.
authors: Maria P
authors: MP
authors: Ferraz
authors: i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; FEUP - Faculdade de Engenharia da Universidade do Porto, Departamento de Engenharia Metalúrgica e Materiais, Porto, Portugal.
authors: Fernando J
authors: FJ
authors: Monteiro
authors: Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dentária, Universidade do Porto, Porto, Portugal.
authors: Maria H
authors: MH
authors: Fernandes
authors: School of Chemical Engineering, University of Campinas, Campinas, Brazil.
authors: Marisa M
authors: MM
authors: Beppu
authors: POLYMAT University of the Basque Country UPV/EHU, Donostia-San Sebastian, Spain.
authors: Daniele
authors: D
authors: Mantione
authors: POLYMAT University of the Basque Country UPV/EHU, Donostia-San Sebastian, Spain. Electronic address: haritz.sardon@ehu.es.
authors: Haritz
authors: H
authors: Sardon
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001861:Bone Regeneration; D002460:Cell Line; D017886:Durapatite / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D006046:Gold; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010006:Osteoblasts / Q000187:drug effects; D012834:Silver; D023822:Tissue Engineering
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D012834:Silver; D006046:Gold; D005349:Fibroins; D017886:Durapatite
keywords: Antimicrobial activity; Gold nanoparticles; Nanohydroxyapatite; Silk fibroin; Silver nanoparticles