title: TEMPO-mediated oxidation on galactomannan: Gal/Man ratio and chain flexibility dependence.
abstract: Guar (GG) and locust bean (LBG) galactomannans (GMs) oxidation at C-6 was performed with catalyst TEMPO, in which the reaction progress was monitored by consume of NaOH solution. The products were characterized by spectroscopic analysis, infrared, and (1)H-nuclear magnetic resonance, confirming the presence of aldehydes groups as intermediate of reaction to carboxylic acid. From high performance anion exchange chromatography with pulsed amperometric detection Man/Gal molar ratio was determined and demonstrated a preference to oxidize Man during the reaction on both GMs, following a first order kinetics of oxidation. The comparative macromolecular behavior of native and oxidized GMs was obtained through the analysis by high performance size exclusion chromatography, and the persistence length (Lp) was 6nm and 4nm to native LBG and GG, respectively. A more accessible OH-6 at mannose residue in LBG could be related with a two times faster reaction than GG. The selective oxidation with catalyst TEMPO proved to be efficient to increase the flexibility of the GMs during oxidation. Short reaction time and β-elimination process were mainly observed to LBG, probably due to a more favorable oxidation access to the polysaccharide main chain.
authors: BioPol Laboratory, Chemistry Department, Federal University of Paraná, Coronel F. H. dos Santos Street, 210, 81531-980 Curitiba, Paraná, Brazil.
authors: Caroline Novak
authors: CN
authors: Sakakibara
authors: BioPol Laboratory, Chemistry Department, Federal University of Paraná, Coronel F. H. dos Santos Street, 210, 81531-980 Curitiba, Paraná, Brazil.
authors: Maria Rita
authors: MR
authors: Sierakowski
authors: BioPol Laboratory, Chemistry Department, Federal University of Paraná, Coronel F. H. dos Santos Street, 210, 81531-980 Curitiba, Paraná, Brazil.
authors: Neoli
authors: N
authors: Lucyszyn
authors: BioPol Laboratory, Chemistry Department, Federal University of Paraná, Coronel F. H. dos Santos Street, 210, 81531-980 Curitiba, Paraná, Brazil. Electronic address: rilton@quimica.ufpr.br.
authors: Rilton Alves
authors: RA
authors: de Freitas
keywords: Galactomannans; Guar gum; HPSEC; Locust bean gum; Selective C-6 oxidation; TEMPO