title: [AFM fishing of proteins under impulse electric field].
abstract: A combination of (atomic force microscopy)-based fishing (AFM-fishing) and mass spectrometry allows to capture protein molecules from solutions, concentrate and visualize them on an atomically flat surface of the AFM chip and identify by subsequent mass spectrometric analysis. In order to increase the AFM-fishing efficiency we have applied pulsed voltage with the rise time of the front of about 1 ns to the AFM chip. The AFM-chip was made using a conductive material, highly oriented pyrolytic graphite (HOPG). The increased efficiency of AFM-fishing has been demonstrated using detection of cytochrome b5 protein. Selection of the stimulating pulse with a rise time of 1 ns, corresponding to the GHz frequency range, by the effect of intrinsic emission from water observed in this frequency range during water injection into the cell.
authors: Institute of Biomedical Chemistry, Moscow, Russia.
authors: Yu D
authors: YD
authors: Ivanov
authors: Institute of Biomedical Chemistry, Moscow, Russia.
authors: T O
authors: TO
authors: Pleshakova
authors: Institute of Biomedical Chemistry, Moscow, Russia.
authors: K A
authors: KA
authors: Malsagova
authors: Institute of Biomedical Chemistry, Moscow, Russia.
authors: A L
authors: AL
authors: Kaysheva
authors: Institute of Biomedical Chemistry, Moscow, Russia.
authors: A T
authors: AT
authors: Kopylov
authors: Institute of Biomedical Chemistry, Moscow, Russia.
authors: A A
authors: AA
authors: Izotov
authors: Foundation of Perspective Technologies and Innovations, Moscow, Russia.
authors: V Yu
authors: VY
authors: Tatur
authors: RES LTD. Moscow, Russia.
authors: S G
authors: SG
authors: Vesnin
authors: Skryabin Moscow State Academy of Veterinary Medicine and Biotechnjlogy (FSGEI of HPE), Moscow, Russia.
authors: N D
authors: ND
authors: Ivanova
authors: Joint Institute for High Temperatures of RAS, Moscow, Russia.
authors: V S
authors: VS
authors: Ziborov
authors: Institute of Biomedical Chemistry, Moscow, Russia.
authors: A I
authors: AI
authors: Archakov
mesh_terms: D015786:Cytochromes b5 / Q000737:chemistry*; D004574:Electromagnetic Fields; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D020543:Proteome / Q000737:chemistry*
chemical_list: D020543:Proteome; D015786:Cytochromes b5
keywords: detection of low-copied proteins; protein fishing