title: Cigarette smoke-induced chronic obstructive pulmonary disease is attenuated by CCL20-blocker: a rat model.
abstract: OBJECTIVE
  To evaluate whether the effect of dendritic cells (DCs) on chronic obstructive pulmonary disease (COPD) can be relieved by blocking CCL20.
  
  
  METHODS
  30 Wistar rats were randomly divided into three groups: control, COPD, and COPD treated with CCL20 monoclonal antibody. In the latter two groups, COPD was induced by four-week cigarette smoke exposure and trachea injection of lipopolysaccharide solution on two occasions. CCL20 monoclonal antibody was injected intraperitoneally on the first day. All animals were sacrificed on the 29th day. Pathomorphology of the lung and bronchiole was analyzed using hematoxylin and eosin staining. The CCR6 content in the bronchoalveolar lavage fluid was detected using ELISA. DC distribution in the lung was examined by immunohistochemistry for OX62.
  
  
  RESULTS
  COPD rat models showed pathological alterations similar to those in COPD patients. DCs, CCR6, and the severity of emphysema were significantly increased in the COPD group than in controls (all P values <0.001), and they were significantly reduced after anti-CCL20 treatment compared with the COPD group (all P values <0.05).
  
  
  CONCLUSIONS
  The interaction between CCR6 and its ligand CCL20 promotes the effect of DCs in the COPD pathogenesis, which can be reduced by blocking CCL20.
authors: Desheng
authors: D
authors: Sun
authors: Yao Ouyang, Department of Respiratory Medicine, Affiliated Hospital of Zunyi Medical College, Dalian Road 149, Zunyi City, Guizhou Province, China, josefriend_sun@163.com.
authors: Yao
authors: Y
authors: Ouyang
authors: Yanhui
authors: Y
authors: Gu
authors: Xiansheng
authors: X
authors: Liu
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D054418:Chemokine CCL20 / Q000037:antagonists & inhibitors*; D003713:Dendritic Cells / Q000276:immunology; D004195:Disease Models, Animal; D008168:Lung / Q000503:physiopathology; D008297:Male; D029424:Pulmonary Disease, Chronic Obstructive / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D054399:Receptors, CCR6 / Q000187:drug effects; D012907:Smoking / Q000009:adverse effects*
chemical_list: D054418:Chemokine CCL20; D054399:Receptors, CCR6