title: [Antibodies against modified low-density lipoproteins and their complexes in blood of patients with various manifestations of atherosclerosis].
abstract: The study included 79 patients with coronary artery disease, 25 individuals with preclinical atherosclerosis and 59 healthy controls. Key lipid parameters were examined in all the participants. Levels of antibodies (Abs) against (IgG and IgM) LDL modified by malondialdehyde (MDA), acetic anhydride and hypochlorite, were determined by the enzyme-linked immunosorbent assay (ELISA). Abs specificity was tested by competitive ELISA. Circulating immune complexes (CIC) were isolated by precipitation in polyethylene glycol. Abs to hypochlorite-modified low density lipoprotein (hypochlorite-LDL) were detected in the serum samples. These Abs did not demonstrate cross-reactivity with MDA-modified LDL (MDA-LDL) and acetylated LDL (acetyl-LDL). Patients with coronary artery disease had increased levels of CIC (p<0.0001) and decreased levels of Abs (IgM) to hypochlorite-LDL, compared with healthy controls and patients with preclinical atherosclerosis (p=0.006). A correlation between the levels of Abs (IgG) to the hypochlorite-LDL and Abs to MDA- and acetyl-LDL was found. There was a correlation between the content of the Abs (IgM) to MDA- and acetyl-LDL and the concentration of CIC-cholesterol. Lipid parameters did not correlate with Abs levels.
authors: Institute of Experimental Medicine, Saint Petersburg, Russia.
authors: I V
authors: IV
authors: Belik
authors: Institute of Experimental Medicine, Saint Petersburg, Russia.
authors: A A
authors: AA
authors: Ivantsova
authors: Institute of Experimental Medicine, Saint Petersburg, Russia.
authors: Z E
authors: ZE
authors: Mamedova
authors: Institute of Experimental Medicine, Saint Petersburg, Russia; Saint-Petersburg State University, Saint Petersburg, Russia.
authors: A D
authors: AD
authors: Denisenko
mesh_terms: D000089:Acetic Anhydrides / Q000378:metabolism; D000328:Adult; D000368:Aged; D000906:Antibodies / Q000097:blood / Q000276:immunology / Q000378:metabolism; D050197:Atherosclerosis / Q000097:blood*; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood*; D005260:Female; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D011499:Protein Processing, Post-Translational
chemical_list: D000089:Acetic Anhydrides; D000906:Antibodies; D008077:Lipoproteins, LDL; C031800:acetic anhydride; D008315:Malondialdehyde; D006997:Hypochlorous Acid
keywords: antibodies against modified low-density lipoprotein; atherosclerosis; cholesterol circulating immune complexes; modified low-density lipoprotein