title: Studies on Photocleavage, DNA Binding, Cytotoxicity, and Docking Studies of Ruthenium(II) Mixed Ligand Complexes.
abstract: This article describes the synthesis and characterization of three new Ru(II) polypyridyl complexes including [Ru(phen)2(dpphz)]2+(1), [Ru(bpy)2(dpphz)]2+(2) and [Ru(dmb)2(dpphz)]2+(3) where dpphz = dipyrido[3,2-a:2',3'-c] phenazine-11-hydrazide, phen =1,10-phenanthroline, bpy = 2,2'-bipyridine and dmb = 4,4'-dimethyl2,2'-bipyridine. The binding behaviors of these complexes to calf thymus DNA (CT-DNA) were explored by spectroscopic titrations, viscosity measurements. Results suggest that these complexes can bind to CT-DNA through intercalation. However, their binding strength differs from each other; this may be attributed to difference in the ancillary ligand. The cytotoxicity of 1-3 was evaluated by MTT assay; results indicated that all complexes have significant dose dependent cytotoxicity with HeLa tumor cell line. All complexes exhibited efficient photocleavage of pBR322 DNA upon irradiation. The DNA binding ability of 1-3 was also studied by docking the complexes into B-DNA using docking program.
authors: Department of Chemistry, Osmania University, Hyderabad, India.|School of Chemistry, University of Hyderabad, Hyderabad, India.
authors: Yata Praveen
authors: YP
authors: Kumar
authors: School of Chemistry, University of Hyderabad, Hyderabad, India.|Department of Chemistry, National Dong Hwa University, Hualien, Taiwan, China.
authors: C Shobha
authors: CS
authors: Devi
authors: Department of Chemistry, Osmania University, Hyderabad, India.|School of Chemistry, University of Hyderabad, Hyderabad, India.
authors: A
authors: A
authors: Srishailam
authors: Department of Chemistry, Osmania University, Hyderabad, India.|School of Chemistry, University of Hyderabad, Hyderabad, India.
authors: N
authors: N
authors: Deepika
authors: Department of Chemistry, Osmania University, Hyderabad, India.|School of Chemistry, University of Hyderabad, Hyderabad, India.
authors: V Ravi
authors: VR
authors: Kumar
authors: Department of Chemistry, Osmania University, Hyderabad, India.|School of Chemistry, University of Hyderabad, Hyderabad, India.
authors: P Venkat
authors: PV
authors: Reddy
authors: School of Chemistry, University of Hyderabad, Hyderabad, India.|Department of Biochemistry, Osmania University, Hyderabad, -500007, India.
authors: K
authors: K
authors: Nagasuryaprasad
authors: School of Chemistry, University of Hyderabad, Hyderabad, India.|Department of Biochemistry, Osmania University, Hyderabad, -500007, India.
authors: Surya S
authors: SS
authors: Singh
authors: School of Chemistry, University of Hyderabad, Hyderabad, India. pnagababu@iict.res.in.|Inorganic & Physical Chemistry Division, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad, Telangana State, 500007, India. pnagababu@iict.res.in.
authors: Penumaka
authors: P
authors: Nagababu
authors: Department of Chemistry, Osmania University, Hyderabad, India. ssnsirasani@gmail.com.|School of Chemistry, University of Hyderabad, Hyderabad, India. ssnsirasani@gmail.com.
authors: S
authors: S
authors: Satyanarayana
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001419:Bacteria / Q000187:drug effects; D001665:Binding Sites; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology*; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects* / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D007364:Intercalating Agents / Q000737:chemistry / Q000494:pharmacology; D008024:Ligands; D008826:Microbial Sensitivity Tests; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology*; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D056831:Coordination Complexes; D003603:Cytotoxins; D007364:Intercalating Agents; D008024:Ligands; D009942:Organometallic Compounds; D012428:Ruthenium; D004247:DNA; C427203:calf thymus DNA
keywords: Anticancer activity; DNA-binding; Docking studies; Photocleavage; Polypyridyl hydrazide ligand; Ru(II) complexes