title: Transcriptomic analysis reveals key early events of narciclasine signaling in Arabidopsis root apex.
abstract: Histochemical staining and RNA-seq data demonstrated that the ROS- and plant hormone-regulated stress responses are the key early events of narciclasine signaling in Arabidopsis root cells. Narciclasine, an amaryllidaceae alkaloid isolated from Narcissus tazetta bulbs, employs a broad range of functions on plant development and growth. However, its molecular interactions that modulate these roles in plants are not fully understood. To elucidate the global responses of Arabidopsis roots to short-term narciclasine exposure, we first measured the accumulation of H2O2and O2-with histochemical staining, and then profiled the gene expression pattern in Arabidopsis root tips treated with 0.5 µM narciclasine across different exposure times by RNA-seq. Physiological measurements showed a significant increase in H2O2began at 30-60 min of narciclasine treatment and O2-accumulated by 120 min. Compared with controls, 236 genes were upregulated and 54 genes were downregulated with 2 h of narciclasine treatment, while 968 genes were upregulated and 835 genes were downregulated with 12 h of treatment. The Gene Ontology analysis revealed that the differentially expressed genes were highly enriched during oxidative stress, including those involved in the "regulation of transcription", "response to oxidative stress", "plant-pathogen interaction", "ribonucleotide binding", "plant cell wall organization", and "ribosome biogenesis". Moreover, Kyoto Encyclopedia of Genes and Genomes pathway enrichment statistics suggested that carbohydrate metabolism, amino acid metabolism, amino sugar and nucleotide sugar metabolism, and biosynthesis of phenylpropanoid and secondary metabolites were significantly inhibited by 12 h of narciclasine exposure. Hence, our results demonstrate that hormones and H2O2are important regulators of narciclasine signaling and help to uncover the factors involved in the molecular interplay between narciclasine and phytohormones in Arabidopsis root cells.
authors: School of Life Science, Ningxia University, Yinchuan, 750021, People's Republic of China.|Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan, 030000, People's Republic of China.|Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan, People's Republic of China.
authors: Xiaoning
authors: X
authors: Cao
authors: School of Life Science, Ningxia University, Yinchuan, 750021, People's Republic of China.|New Technology Application, Research and Development Center, Ningxia University, Yinchuan, 750021, People's Republic of China.
authors: Fei
authors: F
authors: Ma
authors: School of Life Science, Ningxia University, Yinchuan, 750021, People's Republic of China.
authors: Tingting
authors: T
authors: Xu
authors: Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan, 030000, People's Republic of China.|Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan, People's Republic of China.
authors: Junjie
authors: J
authors: Wang
authors: Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan, 030000, People's Republic of China.|Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan, People's Republic of China.
authors: Sichen
authors: S
authors: Liu
authors: School of Life Science, Ningxia University, Yinchuan, 750021, People's Republic of China.
authors: Gaihong
authors: G
authors: Li
authors: School of Life Science, Ningxia University, Yinchuan, 750021, People's Republic of China.
authors: Qian
authors: Q
authors: Su
authors: Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan, 030000, People's Republic of China. sxnkyqzj@sina.com.|Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan, People's Republic of China. sxnkyqzj@sina.com.
authors: Zhijun
authors: Z
authors: Qiao
authors: School of Life Science, Ningxia University, Yinchuan, 750021, People's Republic of China. nxf-0324@163.com.
authors: XiaoFan
authors: http://orcid.org/0000-0001-5112-0326
authors: X
authors: Na
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000378:metabolism* / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D018519:Meristem / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D010617:Phenanthridines / Q000378:metabolism* / Q000494:pharmacology; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D016897:Respiratory Burst / Q000187:drug effects; D017423:Sequence Analysis, RNA; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013481:Superoxides / Q000378:metabolism
chemical_list: D047151:Amaryllidaceae Alkaloids; D006360:Heat-Shock Proteins; D010617:Phenanthridines; D010937:Plant Growth Regulators; D013481:Superoxides; C010753:narciclasine; D006861:Hydrogen Peroxide
keywords: Arabidopsis; Hormone; Narciclasine; ROS; Root cell; Signaling