title: Amyloid-β Increases Activity of Proteasomes Capped with 19S and 11S Regulators.
abstract: Accumulation of amyloid-β (Aβ) in neurons accompanies Alzheimer's disease progression. In the cytoplasm Aβ influences activity of proteasomes, the multisubunit protein complexes that hydrolyze the majority of intracellular proteins. However, the manner in which Aβ affects the proteolytic activity of proteasomes has not been established. In this study the effect of Aβ42 and Aβ42 with isomerized Asp7 on activity of different forms of proteasomes has been analyzed. It has been shown that Aβ peptides efficiently reduce activity of the 20S proteasomes, but increase activity of the 20S proteasomes capped with the 19S and/or 11S regulators. Modulation of proteasome activity is mainly determined by the C-terminal segment of Aβ (amino acids 17-42). This study demonstrated an important role of proteasome regulators in the interplay between Aβ and the proteasomes.
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors: Alexey V
authors: AV
authors: Morozov
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors: Alexandra A
authors: AA
authors: Kulikova
authors: Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
authors: Tatiana M
authors: TM
authors: Astakhova
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors: Vladimir A
authors: VA
authors: Mitkevich
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors: Ksenia M
authors: KM
authors: Burnysheva
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors: Alexei A
authors: AA
authors: Adzhubei
authors: Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
authors: Pavel A
authors: PA
authors: Erokhov
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors: Michail B
authors: MB
authors: Evgen'ev
authors: Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
authors: Natalia P
authors: NP
authors: Sharova
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors: Vadim L
authors: VL
authors: Karpov
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors: Alexander A
authors: AA
authors: Makarov
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D006801:Humans; D010446:Peptide Fragments / Q000494:pharmacology*; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); C520308:PSMB11 protein, human; D046988:Proteasome Endopeptidase Complex
keywords: 11S regulator; 19S regulator particle; 20S proteasome; Keywords: Alzheimer’s disease; amyloid-β