title: MiR-630 inhibits invasion and metastasis in esophageal squamous cell carcinoma.
abstract: Esophageal squamous cell carcinoma (ESCC) is among the most aggressive malignancies and has a high incidence in China. MicroRNAs (miRNAs) are small endogenous RNAs that regulate multiple tumorigenic processes, including proliferation, invasion, metastasis and prognosis. Using miRNA expression proﬁling analysis, we found that miR-630 was markedly down-regulated in three ESCC tissue samples compared with that in paired normal esophageal tissues. Differential miR-630 expression was subsequently confirmed using quantitative real-time PCR. To determine whether miR-630 down-regulation could be considered as a diagnostic indicator and adverse prognostic factor, we investigated the association between miR-630 and clinicopathological characteristics in patients with ESCC. It was found that decreased miR-630 expression was associated with poor overall survival in these patients. In addition, we also explored the biological function of miR-630 by targeting Slug and investigated the correlation between miR-630 expression and epithelial-mesenchymal transition (EMT) progression in vivo and in vitro Ectopic miR-630 expression could inhibit proliferation, invasion and metastasis, whereas miR-630 knockdown induced proliferation, invasion, metastasis and EMT traits. Overall, our study supports a role for miR-630 as a critical novel modulator in ESCC.
authors: Department of Medical Oncology, Jinling Hospital, Nanjing 210002, China.
authors: Li
authors: L
authors: Jin
authors: Department of Cardiothoracic Surgery, Jinling Hospital, Nanjing 210002, China.
authors: Jun
authors: J
authors: Yi
authors: Department of Medical Oncology, Jinling Hospital, Nanjing 210002, China.
authors: Yanping
authors: Y
authors: Gao
authors: Department of Medical Oncology, Jinling Hospital, Nanjing 210002, China.
authors: Siqi
authors: S
authors: Han
authors: Department of Medical Oncology, Jinling Hospital, Nanjing 210002, China.
authors: Zhenyue
authors: Z
authors: He
authors: Department of Medical Oncology, Jinling Hospital, Nanjing 210002, China chenlongbang@yeah.net songhaizhu@163.com.
authors: Longbang
authors: L
authors: Chen
authors: Department of Medical Oncology, Jinling Hospital, Nanjing 210002, China chenlongbang@yeah.net songhaizhu@163.com.
authors: Haizhu
authors: H
authors: Song
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology* / Q000556:secondary; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000556:secondary; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology / Q000517:prevention & control; D009362:Neoplasm Metastasis / Q000235:genetics / Q000473:pathology / Q000517:prevention & control; D011379:Prognosis; D000071250:Snail Family Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C548399:MIRN630 microRNA, human; D035683:MicroRNAs; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors
keywords: epithelial–mesenchymal transition; esophageal squamous cell carcinoma; invasion and metastasis; miR-630; suppressor