title: Application of the CRISPR/Cas9 gene editing technique to research on functional genomes of parasites.
abstract: The clustered regularly-interspaced short palindromic repeats (CRISPR) structural family functions as an acquired immune system in prokaryotes. Gene editing techniques have co-opted CRISPR and the associated Cas nucleases to allow for the precise genetic modification of human cells, zebrafish, mice, and other eukaryotes. Indeed, this approach has been used to induce a variety of modifications including directed insertion/deletion (InDel) of bases, gene knock-in, introduction of mutations in both alleles of a target gene, and deletion of small DNA fragments. Thus, CRISPR technology offers a precise molecular tool for directed genome modification with a range of potential applications; further, its high mutation efficiency, simple process, and low cost provide additional advantages over prior editing techniques. This paper will provide an overview of the basic structure and function of the CRISPR gene editing system as well as current and potential applications to research on parasites.
authors: Department of Clinical Laboratory, The Third People's Hospital of Yancheng, Affiliated Yancheng Hospital, School of Medicine, Southeast University, Yancheng 224001, Jiangsu Province, PR China. Electronic address: ybcui1975@hotmail.com.
authors: Yubao
authors: Y
authors: Cui
authors: Department of Laboratory Medicine, Yancheng Health Vocational & Technical College, Yancheng 224006, Jiangsu Province, PR China.
authors: Lili
authors: L
authors: Yu
mesh_terms: D000818:Animals; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D000072669:Gene Editing / Q000379:methods*; D018503:Genome, Protozoan / Q000235:genetics*; D006801:Humans; D007891:Leishmania / Q000235:genetics; D010271:Parasites / Q000235:genetics*; D010961:Plasmodium / Q000235:genetics; D014122:Toxoplasma / Q000235:genetics; D014349:Trypanosoma cruzi / Q000235:genetics
keywords: CRISPR/Cas9 systems; Diagnostics; Gene-editing technique; Parasites; Parasitic disease