title: Molecular cloning, expression analysis, and function of decorin in goat ovarian granulosa cells.
abstract: Decorin (DCN), a component of the extracellular matrix (ECM), participates in ECM assembly and influences cell proliferation and apoptosis in many mammalian tissues and cells. However, expression and function of DCN in the ovary remain unclear. This study cloned the full-length cDNA of goat DCN obtained from the ovary of an adult goat. Sequence analysis revealed that the putative DCN protein shared a highly conserved amino acid sequence with known mammalian homologs. The tissue distribution of DCN mRNA expression was evaluated by real-time PCR, and the results showed that DCN was widely expressed in the tissues of adult goat. Immunohistochemistry results suggested that DCN protein existed in the granulosa cells and oocytes from all types of follicles and theca cells of antral follicles. Moreover, hCG-induced DCN mRNA expression was significantly reduced by the inhibitors of protein kinase A, PI3K, or p38 kinase (P < 0.05), which are key mediators involved in hCG-induced DCN expression. Overexpression of DCN significantly increased apoptosis and blocked cell cycle progression in cultured granulosa cells (P < 0.05). Western blot analysis also showed that overexpression of DCN upregulated the expression levels of p21 protein (P < 0.05), whereas no effects were observed on the expression of Bax and Bcl-2 and on Bcl-2/Bax ratio (P > 0.05). These findings suggested that DCN regulates the apoptosis and cell cycle of granulosa cells.
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China, 712100.
authors: J Y
authors: JY
authors: Peng
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China, 712100.
authors: K X
authors: KX
authors: Gao
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China, 712100.
authors: H Y
authors: HY
authors: Xin
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China, 712100.
authors: P
authors: P
authors: Han
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China, 712100; Department of Animal Engineering, Xuzhou Bioengineering Technical College, Xuzhou, Jiangsu, P.R. China, 221006.
authors: G Q
authors: GQ
authors: Zhu
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China, 712100. Electronic address: caobinyun@126.com.
authors: B Y
authors: BY
authors: Cao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D058575:Decorin / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006041:Goats / Q000502:physiology*; D006107:Granulosa Cells / Q000502:physiology*; D010802:Phylogeny
chemical_list: D058575:Decorin; D004247:DNA
keywords: Apoptosis; Decorin; Goat; Granulosa cells; hCG