title: Immobilization of iron- and manganese-oxidizing bacteria with a biofilm-forming bacterium for the effective removal of iron and manganese from groundwater.
abstract: In this study, three bacteria with high Fe- and Mn-oxidizing capabilities were isolated from groundwater well sludge and identified as Acinetobacter sp., Bacillus megaterium and Sphingobacterium sp. The maximum removal ratios of Fe and Mn (99.75% and 96.69%) were obtained by an optimal combination of the bacteria at a temperature of 20.15°C, pH 7.09 and an inoculum size of 2.08%. Four lab-scale biofilters were tested in parallel for the removal of iron and manganese ions from groundwater. The results indicated that the Fe/Mn removal ratios of biofilter R4, which was inoculated with iron- and manganese-oxidizing bacteria and a biofilm-forming bacterium, were approximately 95% for each metal during continuous operation and were better than the other biofilters. This study demonstrated that the biofilm-forming bacterium could promote the immobilization of the iron- and manganese-oxidizing bacteria on the biofilters and enhance the removal efficiency of iron and manganese ions from groundwater.
authors: College of Resources and Environment, Northeast Agricultural University, Harbin 150030, Heilongjiang, PR China.
authors: Chunyan
authors: C
authors: Li
authors: College of Resources and Environment, Northeast Agricultural University, Harbin 150030, Heilongjiang, PR China.
authors: Shuting
authors: S
authors: Wang
authors: College of Resources and Environment, Northeast Agricultural University, Harbin 150030, Heilongjiang, PR China.
authors: Xiaopeng
authors: X
authors: Du
authors: College of First Clinical Medicine of Harbin Medical University, Harbin 150030, Heilongjiang, PR China.
authors: Xiaosong
authors: X
authors: Cheng
authors: College of Resources and Environment, Northeast Agricultural University, Harbin 150030, Heilongjiang, PR China.
authors: Meng
authors: M
authors: Fu
authors: College of Resources and Environment, Northeast Agricultural University, Harbin 150030, Heilongjiang, PR China.
authors: Ning
authors: N
authors: Hou
authors: College of Resources and Environment, Northeast Agricultural University, Harbin 150030, Heilongjiang, PR China. Electronic address: lidapeng@neau.edu.cn.
authors: Dapeng
authors: D
authors: Li
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D018441:Biofilms; D060587:Groundwater / Q000737:chemistry / Q000382:microbiology*; D007501:Iron / Q000302:isolation & purification / Q000378:metabolism*; D008345:Manganese / Q000302:isolation & purification / Q000378:metabolism*; D012722:Sewage; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D012722:Sewage; D014874:Water Pollutants, Chemical; D008345:Manganese; D007501:Iron
keywords: Biofilm; Biofilter; Groundwater; Immobilization; Iron- and manganese-oxidizing bacteria