title: Photo-controlled binding of MutS to photo-caged DNA duplexes incorporating 4-O-(2-nitrobenzyl) or 4-O-[2-(2-nitrophenyl)propyl]thymidine.
abstract: Mismatch binding protein MutS binding to bulge structure in DNA duplexes was controlled by UV irradiation. 4-O-(2-Nitrobenzyl)thymidine or 4-O-[2-(2-nitrophenyl)propyl]thymidine was incorporated into DNA duplexes a bulged position. The MutS did not bind to the caged DNA duplexes but bound after removing the 2-nitrobenzyl or 2-(2-nitrophenyl)propyl group by photo-irradiation. By using photo-caged DNA duplex, we revealed that binding of MutS to the uncaged DNA downstream of the T7 RNA promoter weakly inhibited transcription by T7 RNA polymerase.
authors: Department of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku Yokohama 226-8501, Japan. Electronic address: kseio@bio.titech.ac.jp.
authors: Kohji
authors: K
authors: Seio
authors: Department of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku Yokohama 226-8501, Japan.
authors: Yurie
authors: Y
authors: Ohno
authors: Department of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku Yokohama 226-8501, Japan.
authors: Kentaro
authors: K
authors: Ohno
authors: Department of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku Yokohama 226-8501, Japan.
authors: Leo
authors: L
authors: Takeshita
authors: Department of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku Yokohama 226-8501, Japan.
authors: Takashi
authors: T
authors: Kanamori
authors: Department of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku Yokohama 226-8501, Japan.
authors: Yoshiaki
authors: Y
authors: Masaki
authors: Department of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku Yokohama 226-8501, Japan.
authors: Mitsuo
authors: M
authors: Sekine
mesh_terms: D004247:DNA / Q000378:metabolism*; D051719:MutS DNA Mismatch-Binding Protein / Q000378:metabolism*; D011485:Protein Binding; D013936:Thymidine / Q000737:chemistry / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D004247:DNA; D051719:MutS DNA Mismatch-Binding Protein; D013936:Thymidine
keywords: DNA-protein interaction; MutS; Photo-cage