title: Plasmid with Colistin Resistance Gene mcr-1 in Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strains Isolated from Pig Slurry in Estonia.
abstract: A plasmid carrying the colistin resistance gene mcr-1 was isolated from a pig slurry sample in Estonia. The gene was present on a 33,311-bp plasmid of the IncX4 group. mcr-1 is the only antibiotic resistance gene on the plasmid, with the other genes mainly coding for proteins involved in conjugative DNA transfer (taxA, taxB, taxC, trbM, and the pilX operon). The plasmid pESTMCR was present in three phylogenetically very different Escherichia coli strains, suggesting that it has high potential for horizontal transfer.
authors: Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
authors: Age
authors: A
authors: Brauer
authors: Institute of Microbiology, University of Tartu, Tartu, Estonia.
authors: Kaidi
authors: K
authors: Telling
authors: Institute of Technology, University of Tartu, Tartu, Estonia.
authors: Mailis
authors: M
authors: Laht
authors: Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Tartu, Estonia.
authors: Piret
authors: P
authors: Kalmus
authors: Institute of Microbiology, University of Tartu, Tartu, Estonia.
authors: Irja
authors: I
authors: Lutsar
authors: Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.
authors: Maido
authors: M
authors: Remm
authors: Institute of Technology, University of Tartu, Tartu, Estonia.
authors: Veljo
authors: V
authors: Kisand
authors: Institute of Technology, University of Tartu, Tartu, Estonia tanel.tenson@ut.ee.
authors: Tanel
authors: T
authors: Tenson
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003091:Colistin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics*; D004957:Estonia; D000072480:Farms; D005260:Female; D008372:Manure / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D013552:Swine / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; C000614037:MCR-1 protein, E coli; D008372:Manure; D001618:beta-Lactamases; D003091:Colistin