title: Sindbis Virus Can Exploit a Host Antiviral Protein To Evade Immune Surveillance.
abstract: Viral infection induces production of type I interferons (IFNs), which stimulate the expression of a variety of antiviral factors to inhibit viral replication. To establish effective infection, viruses need to develop strategies to evade the immune responses. A neurovirulent Sindbis virus strain with neuroinvasive properties (SVNI) causes lethal encephalitis in mice, and its replication in cultured cells is inhibited by the zinc finger antiviral protein (ZAP), a host factor that specifically inhibits the replication of certain viruses by binding to the viral mRNAs, repressing the translation of target mRNA, and promoting the degradation of target mRNA. We report here that murine embryonic fibroblast cells from ZAP knockout mice supported more efficient SVNI replication than wild-type cells. SVNI infection of 10-day-old suckling mice led to reduced survival in the knockout mice. Unexpectedly, however, SVNI infection of 23-day-old weanling mice, whose immune system is more developed than that of the suckling mice, resulted in significantly improved survival in ZAP knockout mice. Further analyses revealed that in the weanling knockout mice, SVNI replicated more efficiently in lymphoid tissues at early times postinfection and induced higher levels of IFN production, which restricted viral spread to the central nervous system. Blocking IFN activity through the use of receptor-neutralizing antibodies rendered knockout mice more sensitive to SVNI infection than wild-type mice. These results uncover a mechanism by which SVNI exploits a host antiviral factor to evade innate immune surveillance.
  
  
  OBJECTIVE
  Sindbis virus, a prototypic member of the Alphavirus genus, has been used to study the pathogenesis of acute viral encephalitis in mice for many years. How the virus evades immune surveillance to establish effective infection is largely unknown. ZAP is a host antiviral factor that potently inhibits Sindbis virus replication in cell culture. We show here that infection of ZAP knockout suckling mice with an SVNI led to faster disease progression. However, SVNI infection of weanling mice led to slower disease progression in knockout mice. Further analyses revealed that in weanling knockout mice, SVNI replicated more efficiently in lymphoid tissues at early times postinfection and induced higher levels of interferon production, which restricted viral spread to the central nervous system. These results uncover a mechanism by which SVNI exploits a host antiviral factor to evade innate immune surveillance and allow enhanced neuroinvasion.
authors: CAS Key Laboratory for Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China.
authors: Xinlu
authors: X
authors: Wang
authors: The Rockefeller University, New York, New York, USA.
authors: Melody M H
authors: MMH
authors: Li
authors: Model Animal Research Center, Nanjing University, Nanjing, China.
authors: Jing
authors: J
authors: Zhao
authors: CAS Key Laboratory for Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China.
authors: Shenglan
authors: S
authors: Li
authors: The Rockefeller University, New York, New York, USA.
authors: Margaret R
authors: MR
authors: MacDonald
authors: The Rockefeller University, New York, New York, USA.
authors: Charles M
authors: CM
authors: Rice
authors: Model Animal Research Center, Nanjing University, Nanjing, China.
authors: Xiang
authors: X
authors: Gao
authors: CAS Key Laboratory for Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China gaogx@moon.ibp.ac.cn.
authors: Guangxia
authors: G
authors: Gao
mesh_terms: D018354:Alphavirus Infections / Q000276:immunology* / Q000821:virology; D000818:Animals; D000998:Antiviral Agents / Q000276:immunology*; D002460:Cell Line; D002490:Central Nervous System / Q000276:immunology / Q000821:virology; D006224:Cricetinae; D007113:Immunity, Innate / Q000276:immunology; D007370:Interferon Type I / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016601:RNA-Binding Proteins / Q000276:immunology; D012845:Sindbis Virus / Q000276:immunology*; D014779:Virus Replication / Q000276:immunology
chemical_list: D000998:Antiviral Agents; D007370:Interferon Type I; D016601:RNA-Binding Proteins; C577264:ZAP antiviral protein, mouse