title: Effects of 2-iodohexadecanal in the physiology of thyroid cells.
abstract: Iodide has direct effects on thyroid function. Several iodinated lipids are biosynthesized by the thyroid and they were postulated as intermediaries in the action of iodide. Among them, 2-iodohexadecanal (2-IHDA) has been identified and proposed to play a role in thyroid autoregulation. The aim of this study was to compare the effect of iodide and 2-IHDA on thyroid cell physiology. For this purpose, FRTL-5 thyroid cells were incubated with the two compounds during 24 or 48 h and several thyroid parameters were evaluated such as: iodide uptake, intracellular calcium and H2O2levels. To further explore the molecular mechanism involved in 2-IHDA action, transcript and protein levels of genes involved in thyroid hormone biosynthesis, as well as the transcriptional expression of these genes were evaluated in the presence of iodide and 2-IHDA. The results obtained indicate that 2-IHDA reproduces the action of excess iodide on the "Wolff-Chaikoff" effect as well as on thyroid specific genes transcription supporting its role in thyroid autoregulation.
authors: Nuclear Biochemistry Division, Argentine National Atomic Energy Commission, CONICET, Buenos Aires, Argentina.
authors: Luciano E
authors: LE
authors: Rossich
authors: Nuclear Biochemistry Division, Argentine National Atomic Energy Commission, CONICET, Buenos Aires, Argentina.
authors: Lisa
authors: L
authors: Thomasz
authors: Department of Clinical Biochemistry, School of Chemical Sciences, National University of Cordoba, CONICET, Buenos Aires, Argentina.
authors: Juan P
authors: JP
authors: Nicola
authors: Department of Clinical Biochemistry, School of Chemical Sciences, National University of Cordoba, CONICET, Buenos Aires, Argentina.
authors: Magali
authors: M
authors: Nazar
authors: Nuclear Biochemistry Division, Argentine National Atomic Energy Commission, CONICET, Buenos Aires, Argentina.
authors: Leonardo A
authors: LA
authors: Salvarredi
authors: Nuclear Biochemistry Division, Argentine National Atomic Energy Commission, CONICET, Buenos Aires, Argentina; Department of Human Biochemistry, University of Buenos Aires School of Medicine, CONICET, Buenos Aires, Argentina.
authors: Mario
authors: M
authors: Pisarev
authors: Department of Clinical Biochemistry, School of Chemical Sciences, National University of Cordoba, CONICET, Buenos Aires, Argentina.
authors: Ana M
authors: AM
authors: Masini-Repiso
authors: IRIBHM-IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors: Christiane
authors: C
authors: Christophe-Hobertus
authors: IRIBHM-IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors: Daniel
authors: D
authors: Christophe
authors: Nuclear Biochemistry Division, Argentine National Atomic Energy Commission, CONICET, Buenos Aires, Argentina. Electronic address: juvenal@cnea.gov.ar.
authors: Guillermo J
authors: GJ
authors: Juvenal
mesh_terms: D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003847:Deoxyglucose / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D007454:Iodides / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology* / Q000502:physiology*; D013963:Thyroid Hormones / Q000096:biosynthesis; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000447:Aldehydes; D007454:Iodides; D013963:Thyroid Hormones; D014157:Transcription Factors; C065952:2-iodohexadecanal; D003847:Deoxyglucose; D006861:Hydrogen Peroxide; D002118:Calcium
keywords: 2-Iodohexadecanal; Iodide; Iodolipids; Thyroid; Thyroid cell regulation; Wolff-Chaikoff