title: GmCYP82A3, a Soybean Cytochrome P450 Family Gene Involved in the Jasmonic Acid and Ethylene Signaling Pathway, Enhances Plant Resistance to Biotic and Abiotic Stresses.
abstract: The cytochrome P450 monooxygenases (P450s) represent a large and important enzyme superfamily in plants. They catalyze numerous monooxygenation/hydroxylation reactions in biochemical pathways, P450s are involved in a variety of metabolic pathways and participate in the homeostasis of phytohormones. The CYP82 family genes specifically reside in dicots and are usually induced by distinct environmental stresses. However, their functions are largely unknown, especially in soybean (Glycine max L.). Here, we report the function of GmCYP82A3, a gene from soybean CYP82 family. Its expression was induced by Phytophthora sojae infection, salinity and drought stresses, and treatment with methyl jasmonate (MeJA) or ethephon (ETH). Its expression levels were consistently high in resistant cultivars. Transgenic Nicotiana benthamiana plants overexpressing GmCYP82A3 exhibited strong resistance to Botrytis cinerea and Phytophthora parasitica, and enhanced tolerance to salinity and drought stresses. Furthermore, transgenic plants were less sensitive to jasmonic acid (JA), and the enhanced resistance was accompanied with increased expression of the JA/ET signaling pathway-related genes.
authors: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.
authors: Qiang
authors: Q
authors: Yan
authors: National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.
authors: Xiaoxia
authors: X
authors: Cui
authors: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.
authors: Shuai
authors: S
authors: Lin
authors: National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.
authors: Shuping
authors: S
authors: Gan
authors: National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.
authors: Han
authors: H
authors: Xing
authors: Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.
authors: Daolong
authors: D
authors: Dou
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D060467:Disease Resistance / Q000235:genetics*; D055864:Droughts; D005030:Ethylenes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D054883:Oxylipins / Q000378:metabolism*; D010838:Phytophthora; D010935:Plant Diseases / Q000469:parasitology; D015398:Signal Transduction / Q000235:genetics*; D013025:Glycine max / Q000235:genetics* / Q000469:parasitology; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; C011006:jasmonic acid; D003577:Cytochrome P-450 Enzyme System; C036216:ethylene