title: Dual Enzyme-Triggered In Situ Crosslinkable Gelatin Hydrogels for Artificial Cellular Microenvironments.
abstract: Horseradish peroxidase (HRP) and hydrogen peroxide (H2O2)-mediated crosslinking reaction has become an attractive method to create in situ forming hydrogels. While the crosslinking system has been widely utilized, there are certain issues require improvement to extend their biomedical applications, including creation of stiff hydrogels without compromising cytocompatibility due to initially high concentrations of H2O2. A gelatin-based hydrogels formed through a dual enzyme-mediated crosslinking reaction using HRP and glucose oxidase (GOx) as an H2O2-generating enzyme to gradually supply a radical source in HRP-mediated crosslinking reaction is reported. The physicochemical properties can be controlled by varying enzyme concentrations. Furthermore the hydrogel matrices provide 3D microenvironments for supporting the growth and spreading of human dermal fibroblasts with minimized cytotoxicity, despite the cells being encapsulated within stiff hydrogels. These hydrogels formed with HRP/GOx have great potential as artificial microenvironments for a wide range of biomedical applications.
authors: Department of Molecular Science and Technology, Ajou University, 206 Worldcup-ro, Yeontong-gu, Suwon, 16499, South Korea.
authors: Bae Young
authors: BY
authors: Kim
authors: Department of Molecular Science and Technology, Ajou University, 206 Worldcup-ro, Yeontong-gu, Suwon, 16499, South Korea.
authors: Yunki
authors: Y
authors: Lee
authors: Department of Molecular Science and Technology, Ajou University, 206 Worldcup-ro, Yeontong-gu, Suwon, 16499, South Korea.
authors: Joo Young
authors: JY
authors: Son
authors: Division of Bioengineering, College of Life Sciences and Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon, 22012, South Korea.
authors: Kyung Min
authors: KM
authors: Park
authors: Department of Molecular Science and Technology, Ajou University, 206 Worldcup-ro, Yeontong-gu, Suwon, 16499, South Korea.
authors: Ki Dong
authors: KD
authors: Park
mesh_terms: D002478:Cells, Cultured; D060833:Cellular Microenvironment; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005780:Gelatin / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D008422:Materials Testing
chemical_list: D020100:Hydrogels; D005780:Gelatin; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase
keywords: artificial extracellular matrices; enzyme-mediated crosslinking reaction; glucose oxidase; horseradish peroxidase; in situ crosslinkable hydrogels