title: Patterned Array of Poly(ethylene glycol) Silane Monolayer for Label-Free Detection of Dengue.
abstract: In the present study, the construction of arrays on silicon for naked-eye detection of DNA dengue was demonstrated. The array was created by exposing a polyethylene glycol (PEG) silane monolayer to 254 nm ultraviolet (UV) light through a photomask. Formation of the PEG silane monolayer and photomodifed surface properties was thoroughly characterized by using atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. The results of XPS confirmed that irradiation of ultraviolet (UV) light generates an aldehyde functional group that offers conjugation sites of amino DNA probe for detection of a specific dengue virus target DNA. Employing a gold enhancement process after inducing the electrostatic interaction between positively charged gold nanoparticles and the negatively charged target DNA hybridized to the DNA capture probe allowed to visualize the array with naked eye. The developed arrays demonstrated excellent performance in diagnosis of dengue with a detection limit as low as 10 pM. The selectivity of DNA arrays was also examined using a single base mismatch and noncomplementary target DNA.
authors: Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. norzidarosly@gmail.com.
authors: Nor Zida
authors: NZ
authors: Rosly
authors: Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. ainliah@upm.edu.my.|Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. ainliah@upm.edu.my.
authors: Shahrul Ainliah Alang
authors: SA
authors: Ahmad
authors: Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. jafar@upm.edu.my.
authors: Jaafar
authors: J
authors: Abdullah
authors: Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. azahy@upm.edu.my.|Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. azahy@upm.edu.my.
authors: Nor Azah
authors: NA
authors: Yusof
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D003715:Dengue / Q000175:diagnosis*; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D013194:Staining and Labeling; D055672:Static Electricity; D014466:Ultraviolet Rays
chemical_list: D002593:Cetrimonium Compounds; D012821:Silanes; C490327:poly(ethylene glycol)silane; D011092:Polyethylene Glycols; D006046:Gold; D000077286:Cetrimonium
keywords: DNA dengue; PEG silane monolayer; UV-arrays; gold nanoparticles