title: Fructus Amomi CardamomiExtract Inhibit Coxsackievirus-B3 Induced Myocarditis in Murine Myocarditis Model.
abstract: Coxsackievirus B3 (CVB3) is the main cause of acute myocarditis and dilated cardiomyopathy. Plant extracts are considered as useful materials to develop new antiviral drugs. We had previously selected candidate plant extracts, which showed anti-inflammatory effects. We examined the antiviral effects by using a HeLa cell survival assay. Among these extracts, we chose theAmomi Cardamomi(Amomi) extract, which showed strong antiviral effect and preserved cell survival in CVB3 infection. We investigated the mechanisms underlying the ability ofAmomiextract to inhibit CVB3 infection and replication. HeLa cells were infected by CVB3 with or withoutAmomiextract. Erk and Akt activities, and their correlation with virus replication were observed. Live virus titers in cell supernatants and viral positive- and negative-strand RNA amplification were measured.Amomiextract significantly increased HeLa cell survival in different concentrations (100-10 µg/ml). CVB3 capsid protein VP1 expression (76%) and viral protease 2A-induced eIF4G1 cleavage (70%) were significantly decreased inAmomiextract (100 µg/ml) treated cells. The levels of positive- (20%) and negative-strand (80%) RNA were dramatically decreased compared with the control, as revealed by reverse transcription-PCR. In addition,Amomiextract improved mice survival (51% vs 26%) and dramatically reduced heart inflammation in a CVB3-induced myocarditis mouse model. These results suggested thatAmomiextract significantly inhibitedEnterovirusreplication and myocarditis damage.Amomimay be developed as a therapeutic drug forEnterovirus.
authors: Department of Biomedical Science, Jungwon University, Goesan 28024, Republic of Korea.
authors: Yun-Gyeong
authors: YG
authors: Lee
authors: Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology, Cheonju 28116, Republic of Korea.
authors: Jung-Ho
authors: JH
authors: Park
authors: Cardiac and Vascular Center, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Republic of Korea.
authors: Eun-Seok
authors: ES
authors: Jeon
authors: College of Herbal Bio-Industry, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors: Jin-Hee
authors: JH
authors: Kim
authors: Department of Biomedical Science, Jungwon University, Goesan 28024, Republic of Korea.
authors: Byung-Kwan
authors: BK
authors: Lim
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage*; D003384:Coxsackievirus Infections / Q000188:drug therapy* / Q000821:virology; D004195:Disease Models, Animal; D028523:Elettaria / Q000737:chemistry*; D029822:Enterovirus B, Human / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000188:drug therapy* / Q000821:virology; D010936:Plant Extracts / Q000008:administration & dosage*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts
keywords: Coxsackievirus B3; Enterovirus; antiviral effect; myocarditis; plant extract