title: Characterization of mammary epithelial stem/progenitor cells and their changes with aging in common marmosets.
abstract: Age is the number one risk factor for breast cancer, yet the underlying mechanisms are unexplored. Age-associated mammary stem cell (MaSC) dysfunction is thought to play an important role in breast cancer carcinogenesis. Non-human primates with their close phylogenetic relationship to humans provide a powerful model system to study the effects of aging on human MaSC. In particular, the common marmoset monkey (Callithrix jacchus) with a relatively short life span is an ideal model for aging research. In the present study, we characterized for the first time the mammary epithelial stem/progenitor cells in the common marmoset. The MaSC-enriched cells formed four major types of morphologically distinct colonies when cultured on plates pre-seeded with irradiated NIH3T3 fibroblasts, and were also capable of forming mammospheres in suspension culture and subsequent formation of 3D organoids in Matrigel culture. Most importantly, these 3D organoids were found to contain stem/progenitor cells that can undergo self-renewal and multi-lineage differentiation both in vitro and in vivo. We also observed a significant decrease of luminal-restricted progenitors with age. Our findings demonstrate that common marmoset mammary stem/progenitor cells can be isolated and quantified with established in vitro and in vivo assays used for mouse and human studies.
authors: Institute of Environmental Safety and Human Health, School of Laboratory Medicine and Life Science, Wenzhou Medical University, University Town, Wenzhou 325035, China.|Department of Cellular &Structural Biology, University of Texas Health Science Center, San Antonio, Texas 78229, United States.
authors: Anqi
authors: A
authors: Wu
authors: Institute of Environmental Safety and Human Health, School of Laboratory Medicine and Life Science, Wenzhou Medical University, University Town, Wenzhou 325035, China.|Department of Cellular &Structural Biology, University of Texas Health Science Center, San Antonio, Texas 78229, United States.
authors: Qiaoxiang
authors: Q
authors: Dong
authors: Institute of Environmental Safety and Human Health, School of Laboratory Medicine and Life Science, Wenzhou Medical University, University Town, Wenzhou 325035, China.|Department of Cellular &Structural Biology, University of Texas Health Science Center, San Antonio, Texas 78229, United States.
authors: Hui
authors: H
authors: Gao
authors: Institute of Environmental Safety and Human Health, School of Laboratory Medicine and Life Science, Wenzhou Medical University, University Town, Wenzhou 325035, China.|Department of Cellular &Structural Biology, University of Texas Health Science Center, San Antonio, Texas 78229, United States.
authors: Yuanshuo
authors: Y
authors: Shi
authors: Institute of Environmental Safety and Human Health, School of Laboratory Medicine and Life Science, Wenzhou Medical University, University Town, Wenzhou 325035, China.|Department of Cellular &Structural Biology, University of Texas Health Science Center, San Antonio, Texas 78229, United States.
authors: Yuanhong
authors: Y
authors: Chen
authors: Institute of Environmental Safety and Human Health, School of Laboratory Medicine and Life Science, Wenzhou Medical University, University Town, Wenzhou 325035, China.|Department of Cellular &Structural Biology, University of Texas Health Science Center, San Antonio, Texas 78229, United States.
authors: Fuchuang
authors: F
authors: Zhang
authors: Department of Cellular &Structural Biology, University of Texas Health Science Center, San Antonio, Texas 78229, United States.|Cancer Therapy and Research Center, University of Texas Health Science Center, San Antonio, Texas 78229, United States.
authors: Abhik
authors: A
authors: Bandyopadhyay
authors: Institute of Environmental Safety and Human Health, School of Laboratory Medicine and Life Science, Wenzhou Medical University, University Town, Wenzhou 325035, China.
authors: Danhan
authors: D
authors: Wang
authors: Flow Cytometry Facility, University of Texas Health Science Center, San Antonio, Texas 78229, United States.
authors: Karla M
authors: KM
authors: Gorena
authors: Institute of Environmental Safety and Human Health, School of Laboratory Medicine and Life Science, Wenzhou Medical University, University Town, Wenzhou 325035, China.
authors: Changjiang
authors: C
authors: Huang
authors: Department of Cellular &Structural Biology, University of Texas Health Science Center, San Antonio, Texas 78229, United States.|Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center, San Antonio, Texas 78229, United States.|Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, 78245, United States.
authors: Suzette
authors: S
authors: Tardif
authors: Center for Pregnancy and Newborn Research, University of Texas Health Science Center, San Antonio, Texas 78229, United States.
authors: Peter W
authors: PW
authors: Nathanielsz
authors: The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325035, China.|Department of Cellular &Structural Biology, University of Texas Health Science Center, San Antonio, Texas 78229, United States.|Cancer Therapy and Research Center, University of Texas Health Science Center, San Antonio, Texas 78229, United States.
authors: Lu-Zhe
authors: LZ
authors: Sun
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D002144:Callithrix / Q000502:physiology*; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D004847:Epithelial Cells / Q000502:physiology; D005260:Female; D039001:Integrin alpha Chains / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000502:physiology; D016513:Mice, SCID; D051920:NK Cell Lectin-Like Receptor Subfamily D / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D039001:Integrin alpha Chains; D051920:NK Cell Lectin-Like Receptor Subfamily D