title: Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm.
abstract: An iterative transform method proposed previously for direct phasing of high-solvent-content protein crystals is employed for enhancing the molecular-replacement (MR) algorithm in protein crystallography. Target structures that are resistant to conventional MR due to insufficient similarity between the template and target structures might be tractable with this modified phasing method. Trial calculations involving three different structures are described to test and illustrate the methodology. The relationship of the approach to PHENIX Phaser-MR and MR-Rosetta is discussed.
authors: Department of Physics and Texas Center for Superconductivity, University of Houston, Houston, Texas 77204, USA.
authors: Hongxing
authors: H
authors: He
authors: Department of Physics and Texas Center for Superconductivity, University of Houston, Houston, Texas 77204, USA.
authors: Hengrui
authors: H
authors: Fang
authors: Department of BioSciences, Rice University, Houston, Texas 77005, USA.
authors: Mitchell D
authors: MD
authors: Miller
authors: Department of BioSciences, Rice University, Houston, Texas 77005, USA.
authors: George N
authors: GN
authors: Phillips
authors: Department of Physics and Texas Center for Superconductivity, University of Houston, Houston, Texas 77204, USA.
authors: Wu Pei
authors: WP
authors: Su
keywords: ab initio phasing; hybrid input–output algorithm; molecular replacement; protein crystallography