title: Polyelectrolyte complexes via desalting mixtures of hyaluronic acid and chitosan-Physicochemical study and structural analysis.
abstract: Polyelectrolyte complexes (PECs) were prepared from Chitosan (CS) and Hyaluronic Acid (HYA) homogeneous mixtures of aqueous solutions. The method consisted of preparing a homogeneous mixture of the two polysaccharides via charge screening at high salt concentrations. Then, the mixture was dialyzed, leading to the controlled self-assembly of the two polyelectrolytes. Critical parameters like the chitosan degree of acetylation (DA) and molar mass (Mw), the residual salt concentration and the molar charge ratio r=nNH3(+) (CS)/nCOO(-) (HYA) accounted for the transition from homogeneous aqueous solutions to colloidal suspensions (r=0.1) or gel coacervates (r=0.5). The influence of the DA and Mw of CS was evaluated by visual observations, light scattering and rheological measurements. For low values of r, Small Angle X-ray Scattering (SAXS) experiments revealed that the HYA nanostructure was weakly affected by the presence of PECs. On the contrary, the structure was impacted when increasing r, revealing a heterogeneous aggregate morphology with ladder-like chain interactions.
authors: Univ. Lyon, Université Claude Bernard Lyon 1, CNRS, IMP, UMR 5223, 15 Boulevard Latarjet, F-69622, Villeurbanne, France; Laboratoire ObvieLine, Sinclair IS Pharma, 8 Chemin du Jubin, F-69570, Dardilly, France.
authors: G
authors: G
authors: Lalevée
authors: Univ. Lyon, Université Claude Bernard Lyon 1, CNRS, IMP, UMR 5223, 15 Boulevard Latarjet, F-69622, Villeurbanne, France.
authors: G
authors: G
authors: Sudre
authors: Univ. Lyon, Université Claude Bernard Lyon 1, CNRS, IMP, UMR 5223, 15 Boulevard Latarjet, F-69622, Villeurbanne, France.
authors: A
authors: A
authors: Montembault
authors: Laboratoire ObvieLine, Sinclair IS Pharma, 8 Chemin du Jubin, F-69570, Dardilly, France.
authors: J
authors: J
authors: Meadows
authors: Laboratoire ObvieLine, Sinclair IS Pharma, 8 Chemin du Jubin, F-69570, Dardilly, France.
authors: S
authors: S
authors: Malaise
authors: Univ. Lyon, Université Claude Bernard Lyon 1, CNRS, IMP, UMR 5223, 15 Boulevard Latarjet, F-69622, Villeurbanne, France.
authors: A
authors: A
authors: Crépet
authors: Univ. Lyon, Université Claude Bernard Lyon 1, CNRS, IMP, UMR 5223, 15 Boulevard Latarjet, F-69622, Villeurbanne, France.
authors: L
authors: L
authors: David
authors: Univ. Lyon, Université Claude Bernard Lyon 1, CNRS, IMP, UMR 5223, 15 Boulevard Latarjet, F-69622, Villeurbanne, France. Electronic address: Thierry.Delair@univ-lyon1.fr.
authors: T
authors: T
authors: Delair
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry; D000071228:Polyelectrolytes / Q000737:chemistry*; D012212:Rheology; D012492:Salts / Q000737:chemistry; D053838:Scattering, Small Angle; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D000071228:Polyelectrolytes; D012492:Salts; D006820:Hyaluronic Acid; D048271:Chitosan
keywords: Chitosan; Coacervate; Hyaluronic acid; Hydrogel; Nanostructure; Polyelectrolyte complexes