title: Second-degree burns with six etiologies treated with autologous noncultured cell-spray grafting.
abstract: Partial and deep partial-thickness burn wounds present a difficult diagnosis and prognosis that makes the planning for a conservative treatment versus mesh grafting problematic. A non-invasive treatment strategy avoiding mesh grafting is often chosen by practitioners based on their clinical and empirical evidence. However, a delayed re-epithelialization after conservative treatment may extend the patient's hospitalization period, increase the risk of infection, and lead to poor functional and aesthetic outcome. Early spray grafting, using non-cultured autologous cells, is under discussion for partial and deep partial-thickness wounds to accelerate the re-epithelialization process, reducing the healing time in the hospital, and minimizing complications. To address planning for future clinical studies on this technology, suitable indications will be interesting. We present case information on severe second-degree injuries after gas, chemical, electrical, gasoline, hot water, and tar scalding burns showing one patient per indication. The treatment results with autologous non-cultured cells, support rapid, uncomplicated re-epithelialization with aesthetically and functionally satisfying outcomes. Hospital stays averaged 7.6±1.6 days. Early autologous cell-spray grafting does not preclude or prevent simultaneous or subsequent traditional mesh autografting when indicated on defined areas of full-thickness injury.
authors: Department of Surgery and Bioengineering, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Roger
authors: R
authors: Esteban-Vives
authors: School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Myung S
authors: MS
authors: Choi
authors: Department of Surgery and Bioengineering, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Matthew T
authors: MT
authors: Young
authors: Department of Surgery and Bioengineering, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Patrick
authors: P
authors: Over
authors: The University of Pittsburgh Medical Center, UPMC Mercy Hospital Trauma Services and Burn Center, Pittsburgh, PA, USA.
authors: Jenny
authors: J
authors: Ziembicki
authors: The University of Pittsburgh Medical Center, UPMC Mercy Hospital Trauma Services and Burn Center, Pittsburgh, PA, USA.
authors: Alain
authors: A
authors: Corcos
authors: Department of Surgery and Bioengineering, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: jgerlach@pitt.edu.
authors: Jörg C
authors: JC
authors: Gerlach
mesh_terms: D000293:Adolescent; D000328:Adult; D001134:Arm Injuries / Q000628:therapy*; D002056:Burns / Q000628:therapy*; D002057:Burns, Chemical / Q000628:therapy; D002058:Burns, Electric / Q000628:therapy; D002469:Cell Separation / Q000379:methods*; D010450:Endopeptidases; D006230:Hand Injuries / Q000628:therapy*; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D062326:Re-Epithelialization; D016038:Skin Transplantation / Q000379:methods*; D013898:Thoracic Injuries / Q000628:therapy*; D014182:Transplantation, Autologous; D014357:Trypsin; D014945:Wound Healing
chemical_list: D010450:Endopeptidases; D014357:Trypsin; C023139:dispase
keywords: Burns; Cell-spray grafting; Keratinocytes; Re-epithelialization; Wound healing