title: Heat-labile Escherichia coli toxin enhances the induction of allergen-specific IgG antibodies in epicutaneous patch vaccination.
abstract: Epicutaneous allergen-specific immunotherapy (EPIT) is proposed as an alternative route for allergen-specific immunotherapy (AIT). The induction of allergen-specific blocking IgG antibodies represents an important mechanism underlying AIT, but has not been investigated for EPIT. Here, we compared the induction of allergen-specific blocking IgG in outbred guinea pigs which had been immunized with recombinant birch pollen allergen Bet v 1 using patch delivery system (PDS) with or without heat-labile toxin (LT) from Escherichia coli or subcutaneously with aluminum hydroxide (Alum)-adsorbed rBet v 1. Only subcutaneous immunization with Alum-adsorbed rBet v 1 and epicutaneous administration of rBet v 1 with PDS in combination with LT from E. coli induced allergen-specific IgG antibodies blocking allergic patients' IgE, but not immunization with rBet v 1 via PDS alone. Our results suggest that patch vaccination with rBet v 1 in combination with LT may be a promising strategy for allergen-specific immunotherapy against birch pollen allergy.
authors: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors: C R
authors: CR
authors: Cabauatan
authors: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors: R
authors: R
authors: Campana
authors: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors: K
authors: K
authors: Niespodziana
authors: Valneva Austria GmbH, Campus Vienna Biocenter, Vienna, Austria.
authors: C
authors: C
authors: Reinisch
authors: Valneva Austria GmbH, Campus Vienna Biocenter, Vienna, Austria.
authors: U
authors: U
authors: Lundberg
authors: Valneva Austria GmbH, Campus Vienna Biocenter, Vienna, Austria.
authors: A
authors: A
authors: Meinke
authors: Biomay AG, Vienna, Austria.
authors: R
authors: R
authors: Henning
authors: Biomay AG, Vienna, Austria.
authors: A
authors: A
authors: Neubauer
authors: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors: R
authors: R
authors: Valenta
mesh_terms: D000485:Allergens / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D052179:Antigens, Plant / Q000276:immunology; D001427:Bacterial Toxins / Q000276:immunology*; D003888:Desensitization, Immunologic / Q000379:methods; D004768:Enterotoxins / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D057968:Transdermal Patch; D014611:Vaccination / Q000379:methods
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D007073:Immunoglobulin E; C027538:heat-labile enterotoxin, E coli
keywords: allergen-specific immunotherapy; epicutaneous allergen-specific immunotherapy; heat-labile Escherichia coli toxin; patch delivery system; rBet v 1