title: In Vitro High-Throughput RNAi Screening to Accelerate the Process of Target Identification and Drug Development.
abstract: High-throughput RNA interference (HT-RNAi) is a powerful tool that can be used to knock down gene expression in order to identify novel genes and pathways involved in many cellular processes. It is a systematic, yet unbiased, approach to identify essential or synthetic lethal genes that promote cell survival in diseased cells as well as genes that confer resistance or sensitivity to drug treatment. This information serves as a foundation for enhancing current treatments for cancer and other diseases by identifying new drug targets, uncovering potential combination therapies, and helping clinicians match patients with the most effective treatment based on genetic information. Here, we describe the method of performing an in vitro HT-RNAi screen using chemically synthesized siRNA.
authors: Cancer and Cell Biology Division (CCB), Translational Genomics Research Institute (TGen), 445 N. Fifth Street, Phoenix, AZ, 85004, USA. hyin@tgen.org.
authors: Hongwei
authors: H
authors: Yin
authors: Cancer and Cell Biology Division (CCB), Translational Genomics Research Institute (TGen), 445 N. Fifth Street, Phoenix, AZ, 85004, USA.
authors: Michelle
authors: M
authors: Kassner
mesh_terms: D055808:Drug Discovery / Q000379:methods*; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D020043:Genes, Essential; D057166:High-Throughput Screening Assays / Q000379:methods*; D034622:RNA Interference; D034741:RNA, Small Interfering
chemical_list: D034741:RNA, Small Interfering
keywords: Drug development; Drug sensitizer; Essential gene; Functional genomics; Gene knockdown; Gene silencing; High-throughput; RNA interference (RNAi); Synthetic lethal; Target discovery; siRNA