title: Proteomic comparison reveals the contribution of chloroplast to salt tolerance of a wheat introgression line.
abstract: We previously bred a salt tolerant wheat cv. SR3 with bread wheat cv. JN177 as the parent via asymmetric somatic hybridization, and found that the tolerance is partially attributed to the superior photosynthesis capacity. Here, we compared the proteomes of two cultivars to unravel the basis of superior photosynthesis capacity. In the maps of two dimensional difference gel electrophoresis (2D-DIGE), there were 26 differentially expressed proteins (DEPs), including 18 cultivar-based and 8 stress-responsive ones. 21 of 26 DEPs were identified and classified into four categories, including photosynthesis, photosynthesis system stability, linolenic acid metabolism, and protein synthesis in chloroplast. The chloroplast localization of some DEPs confirmed that the identified DEPs function in the chloroplast. The overexpression of a DEP enhanced salt tolerance in Arabidopsis thaliana. In line with these data, it is concluded that the contribution of chloroplast to high salinity tolerance of wheat cv. SR3 appears to include higher photosynthesis efficiency by promoting system protection and ROS clearance, stronger production of phytohormone JA by enhancing metabolism activity, and modulating the in chloroplast synthesis of proteins.
authors: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shandanan Road, Jinan, Shandong 250100, China.
authors: Wenjing
authors: W
authors: Xu
authors: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shandanan Road, Jinan, Shandong 250100, China.
authors: Hongjun
authors: H
authors: Lv
authors: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shandanan Road, Jinan, Shandong 250100, China.
authors: Mingming
authors: M
authors: Zhao
authors: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shandanan Road, Jinan, Shandong 250100, China.
authors: Yongchao
authors: Y
authors: Li
authors: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shandanan Road, Jinan, Shandong 250100, China.
authors: Yueying
authors: Y
authors: Qi
authors: Bio-Tech Research Center, Shandong Academy of Agricultural Science, Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crop, Jinan, 250100, China.
authors: Zhenying
authors: Z
authors: Peng
authors: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shandanan Road, Jinan, Shandong 250100, China.
authors: Guangmin
authors: G
authors: Xia
authors: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, 27 Shandanan Road, Jinan, Shandong 250100, China.
authors: Mengcheng
authors: M
authors: Wang
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D002736:Chloroplasts / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D010788:Photosynthesis / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D040901:Proteomics; D055051:Salt-Tolerant Plants / Q000235:genetics* / Q000254:growth & development; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D013312:Stress, Physiological / Q000235:genetics; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
chemical_list: D010940:Plant Proteins