title: Lipids, hemoproteins and carotenoids in alive Rhodotorula mucilaginosa cells under pesticide decomposition - Raman imaging study.
abstract: Various species of yeasts are gaining attention as producers of nutraceuticals and biofuels and due to their capacity to biodegrade chemical waste. Rhodotorula mucilaginosa is one of the most oleaginous species of yeast, an efficient de novo carotenoid producer and was reported to be capable of decomposing of organic pesticides. In this work we studied the influence of a toxic pesticide, diazinone, on production of storage (lipids) and protective (carotenoids, hemoproteins) compounds by Rh. mucilaginosa alive cells with the help of Raman imaging. It occurred that the yeast in non-oleaginous phase and aerobic environment was rich in carotenoids and their level increased significantly under incubation with diazinone, while anaerobic environment resulted in production of both carotenoids and hemoproteins and the level of the latter decreased under the influence of the pesticide. For yeasts in oleaginous phase, it was concluded that lipid production (via triggering of NAD+accumulation and increase of the NO level) resulted in nitrosative stress leading to flavohemoprotein synthesis and was associated with the increase of the mitochondrial activity.
authors: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland; Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland.
authors: Marta Z
authors: MZ
authors: Pacia
authors: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland.
authors: Jan
authors: J
authors: Pukalski
authors: Institute of Environmental Sciences and Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
authors: Katarzyna
authors: K
authors: Turnau
authors: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland; Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland.
authors: Malgorzata
authors: M
authors: Baranska
authors: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Krakow, Poland; Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland. Electronic address: kaczor@chemia.uj.edu.pl.
authors: Agnieszka
authors: A
authors: Kaczor
mesh_terms: D056804:Biofuels; D002338:Carotenoids / Q000032:analysis / Q000096:biosynthesis*; D003976:Diazinon / Q000378:metabolism / Q000494:pharmacology; D006420:Hemeproteins / Q000032:analysis / Q000096:biosynthesis*; D007218:Industrial Microbiology; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis*; D010575:Pesticides / Q000378:metabolism* / Q000494:pharmacology; D012248:Rhodotorula / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D013059:Spectrum Analysis, Raman
chemical_list: D056804:Biofuels; D006420:Hemeproteins; D008055:Lipids; D010575:Pesticides; D002338:Carotenoids; D003976:Diazinon
keywords: Biofuels; Carotenoids; Flavohemoproteins; Lipids; Raman imaging; Raman signature of life