title: DNA-binding studies of the natural β-carboline eudistomin U.
abstract: Eudistomin U is a member of the β-carboline class of heterocyclic amine-containing molecules that are capable of binding to DNA. The structure of eudistomin U is unique since it contains an indole ring at the 1-position of the pyridine ring. While simple β-carbolines are reported to intercalate DNA, an examination of the mode of binding of eudistomin U has been lacking. We report preliminary spectroscopic (UV-Vis, thermal denaturation, CD) and calorimetric (DSC) data on the binding of eudistomin U to DNA, which suggest that eudistomin U binds weakly according to a mechanism that is more complicated than other members of its class.
authors: Providence College, Department of Chemistry and Biochemistry, 1 Cunningham Square, Providence, RI 02918, USA.
authors: Jennifer M
authors: JM
authors: Giulietti
authors: Providence College, Department of Chemistry and Biochemistry, 1 Cunningham Square, Providence, RI 02918, USA.
authors: Patrick M
authors: PM
authors: Tate
authors: University of Rhode Island, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, 7 Greenhouse Road, Kingston, RI 02881, USA.
authors: Ang
authors: A
authors: Cai
authors: University of Rhode Island, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, 7 Greenhouse Road, Kingston, RI 02881, USA.
authors: Bongsup
authors: B
authors: Cho
authors: Providence College, Department of Chemistry and Biochemistry, 1 Cunningham Square, Providence, RI 02918, USA. Electronic address: smulcahy@providence.edu.
authors: Seann P
authors: SP
authors: Mulcahy
mesh_terms: D002152:Calorimetry, Differential Scanning; D002243:Carbolines / Q000737:chemistry*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
chemical_list: D002243:Carbolines; C088110:eudistomin U; D004247:DNA; C010262:norharman
keywords: Circular dichroism; DNA; Differential scanning calorimetry; Eudistomin U; Thermal denaturation; UV–Vis; β-Carboline