title: Evidence of ectopic recombination and a repeat-induced point (RIP) mutation in the genome of Sclerotinia sclerotiorum, the agent responsible for white mold.
abstract: Two retrotransposons from the superfamilies Copia and Gypsy named as Copia-LTR_SS and Gypsy-LTR_SS, respectively, were identified in the genomic bank of Sclerotinia sclerotiorum. These transposable elements (TEs) contained direct and preserved long terminal repeats (LTR). Domains related to codified regions for gag protein, integrase, reverse transcriptase and RNAse H were identified in Copia-LTR_SS, whereas in Gypsy-LTR_SS only domains for gag, reverse transcriptase and RNAse H were found. The abundance of identified LTR-Solo suggested possible genetic recombination events in the S. sclerotiorum genome. Furthermore, alignment of the sequences for LTR elements from each superfamily suggested the presence of a RIP (repeat-induced point mutation) silencing mechanism that may directly affect the evolution of this species.
authors: Laboratório de Biologia Molecular de Plantas, Instituto de Biotecnologia Aplicada (BIOAGRO), Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil.
authors: Míriam
authors: M
authors: Goldfarb
authors: Laboratório de Genética Molecular e de Microrganismo, Instituto de Biotecnologia Aplicada (BIOAGRO), Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil.
authors: Mateus Ferreira
authors: MF
authors: Santana
authors: Laboratório de Biologia Molecular de Insetos, Departamento de Biologia Geral, Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil.
authors: Tânia Maria Fernandes
authors: TM
authors: Salomão
authors: Laboratório de Genética Molecular e de Microrganismo, Instituto de Biotecnologia Aplicada (BIOAGRO), Universidade Federal de Viçosa (UFV), 36570-000, Viçosa, MG, Brazil.
authors: Marisa Vieira de
authors: MV
authors: Queiroz
authors: Laboratório de Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, 70790-160, Brasília, DF, Brazil.
authors: Everaldo Gonçalves de
authors: EG
authors: Barros