title: Wnt/β-catenin signaling plays an important role in the protective effects of FDP-Sr against oxidative stress induced apoptosis in MC3T3-E1 cell.
abstract: Strontium fructose 1,6-diphosphate (FDP-Sr) is a new strontium-containing compound. The primary aim of this study was to clarify whether the structure component of FDP-Sr, FDP could benefit the protective effect of Sr (II) against oxidative stress induced apoptosis, and meanwhile to further explore the important role of Wnt/β-catenin signaling in the anti-apoptosis effect of FDP-Sr in response to oxidative stress induced by H2O2 in an osteoblastic MC3T3-E1 cell line. Results showed that FDP-Sr could improve the osteoblastic differentiation under oxidative stress with induced cell proliferation and improved mineralization. The inhibition effect of FDP-Sr on cell apoptosis induced by H2O2 was proved by reduced reactive oxygen species production and activated caspase3. Under oxidative stress, mRNA and protein levels of phospho-β-catenin reduced, while β-catenin increased in the FDP-Sr treatment cell, leaded to the up-regulations of Runx2 and OPG at both mRNA and protein levels, finally improved the differentiation of osteoblasts. By the engagement of Wnt/β-catenin pathway's inhibitor (XAV-939), the protective effects of FDP-Sr on osteoblastic differentiation against oxidative stress were repressed along with inhibited wnt/β-catenin signaling and reduced mRNA and protein levels of Runx2 and OPG. In conclusion, FDP-Sr was demonstrated to protect osteoblast differentiation from oxidative damage induced by H2O2 through up-regulation of Wnt/β-catenin signaling, and FDP in FDP-Sr was able to directly improve the oxidative stress injury through its ROS scavenging ability.
authors: School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, People's Republic of China.
authors: Huan-Huan
authors: HH
authors: Qi
authors: Jiangsu Province of Ficial Hospital, Nanjing 210024, People's Republic of China.
authors: Jun
authors: J
authors: Bao
authors: School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, People's Republic of China. Electronic address: nancyzhang03@hotmail.com.
authors: Qi
authors: Q
authors: Zhang
authors: School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, People's Republic of China. Electronic address: mabo19821221@163.com.
authors: Bo
authors: B
authors: Ma
authors: School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, People's Republic of China.
authors: Gui-Ying
authors: GY
authors: Gu
authors: Jiangsu Province of Ficial Hospital, Nanjing 210024, People's Republic of China.
authors: Peng-Ling
authors: PL
authors: Zhang
authors: Jiangsu Province of Ficial Hospital, Nanjing 210024, People's Republic of China.
authors: Gang
authors: G
authors: Ou-Yang
authors: School of Pharmacy, FMHS, The University of Auckland Private Bag 92019, Auckland 1142, New Zealand.
authors: Zi-Mei
authors: ZM
authors: Wu
authors: School of Life Science & Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, People's Republic of China.
authors: Han-Jie
authors: HJ
authors: Ying
authors: School of Life Science & Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, People's Republic of China.
authors: Ping-Kai
authors: PK
authors: Ou-Yang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D005573:Formycins / Q000737:chemistry*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D012265:Ribonucleotides / Q000737:chemistry*; D015398:Signal Transduction; D051153:Wnt Proteins / Q000378:metabolism*
chemical_list: D005573:Formycins; D012265:Ribonucleotides; D051153:Wnt Proteins; C020332:formycin diphosphate
keywords: Apoptosis; FDP-Sr; Osteoporosis; Oxidative stress; Wnt/β-catenin signaling