title: Development and validation of multivariate calibration methods for simultaneous estimation of Paracetamol, Enalapril maleate and hydrochlorothiazide in pharmaceutical dosage form.
abstract: Three multivariate calibration spectrophotometric methods were developed for simultaneous estimation of Paracetamol (PARA), Enalapril maleate (ENM) and Hydrochlorothiazide (HCTZ) in tablet dosage form; namely multi-linear regression calibration (MLRC), trilinear regression calibration method (TLRC) and classical least square (CLS) method. The selectivity of the proposed methods were studied by analyzing the laboratory prepared ternary mixture and successfully applied in their combined dosage form. The proposed methods were validated as per ICH guidelines and good accuracy; precision and specificity were confirmed within the concentration range of 5-35μgmL-1, 5-40μgmL-1and 5-40μgmL-1of PARA, HCTZ and ENM, respectively. The results were statistically compared with reported HPLC method. Thus, the proposed methods can be effectively useful for the routine quality control analysis of these drugs in commercial tablet dosage form.
authors: University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattishgarh, 492010, India. Electronic address: veena1806@gmail.com.
authors: Veena D
authors: VD
authors: Singh
authors: University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattishgarh, 492010, India.
authors: Sanjay J
authors: SJ
authors: Daharwal
mesh_terms: D000082:Acetaminophen / Q000032:analysis* / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004304:Dosage Forms; D004656:Enalapril / Q000032:analysis* / Q000737:chemistry; D006852:Hydrochlorothiazide / Q000032:analysis* / Q000737:chemistry; D016018:Least-Squares Analysis; D016014:Linear Models; D015999:Multivariate Analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D004304:Dosage Forms; D006852:Hydrochlorothiazide; D000082:Acetaminophen; D004656:Enalapril
keywords: Enalpril maleate; Hydrochlorothiazide; Multivariate calibration methods; Paracetamol