title: Identification of two candidate innate immune genes by transcriptional profiling and RNA interference in mouse mammary gland epithelial cells stimulated with lipopolysaccharide.
abstract: Mammary epithelial cells (MECs) play an important role in immune responses and inflammatory diseases such as mastitis, which is mainly attributed to the activation of Toll-like receptors and the release of cytokines. However, the overall change of gene expression and biological pathways of MECs to microbial factors stimulation remains unknown. Here, we analyzed the gene expression profile in mouse MECs treated with lipopolysaccharide (LPS) for 24 h. Microarray analysis revealed that about 1548 genes differentially expressed, these genes mainly involved in 346 gene ontology terms and 128 molecular pathways, and particularly, some innate immune-associated pathways were significant. By analyzing data for pathway relation network, we prioritized differentially expressed genes with respect to LPS. The importance of changes, indicating that RNA interference-mediated inhibition of two genes identified in this analysis, transforming growth factor beta 1 (Tgf-β1) and platelet-derived growth factor B (Pdgfb), reduced interleukin (IL)-6 and tumor necrosis factor α production respectively, in gene expression was verified. These findings delineate mouse MECs gene response patterns induced by LPS and identify Tgf-β1 and Pdgfb that have been closely related to innate immunity.
authors: a Biochemical Engineering Center of Guizhou Province , Guizhou University , Guiyang , China.
authors: Qiong
authors: Q
authors: Yi
authors: a Biochemical Engineering Center of Guizhou Province , Guizhou University , Guiyang , China.|b Department of Veterinary Medicine, College of Animal Science , Guizhou University , Guiyang , China.
authors: Yu-Kun
authors: YK
authors: Wang
authors: a Biochemical Engineering Center of Guizhou Province , Guizhou University , Guiyang , China.|b Department of Veterinary Medicine, College of Animal Science , Guizhou University , Guiyang , China.
authors: Jiang
authors: J
authors: Feng
authors: a Biochemical Engineering Center of Guizhou Province , Guizhou University , Guiyang , China.|b Department of Veterinary Medicine, College of Animal Science , Guizhou University , Guiyang , China.
authors: Yu-Hao
authors: YH
authors: Wei
authors: a Biochemical Engineering Center of Guizhou Province , Guizhou University , Guiyang , China.
authors: Lu
authors: L
authors: Wang
keywords: Mammary epithelial cells; RNA interference; gene expression; innate immunity; platelet-derived growth factor B; transforming growth factor beta 1