title: DELLA-mediated gibberellin signalling regulates Nod factor signalling and rhizobial infection.
abstract: Legumes develop symbiotic interactions with rhizobial bacteria to form nitrogen-fixing nodules. Bacterial Nod factors (NFs) and plant regulatory pathways modulating NF signalling control rhizobial infections and nodulation efficiency. Here we show that gibberellin (GA) signalling mediated by DELLA proteins inhibits rhizobial infections and controls the NF induction of the infection marker ENOD11 in Medicago truncatula. Ectopic expression of a constitutively active DELLA protein in the epidermis is sufficient to promote ENOD11 expression in the absence of symbiotic signals. We show using heterologous systems that DELLA proteins can interact with the nodulation signalling pathway 2 (NSP2) and nuclear factor-YA1 (NF-YA1) transcription factors that are essential for the activation of NF responses. Furthermore, MtDELLA1 can bind the ERN1 (ERF required for nodulation 1) promoter and positively transactivate its expression. Overall, we propose that GA-dependent action of DELLA proteins may directly regulate the NSP1/NSP2 and NF-YA1 activation of ERN1 transcription to regulate rhizobial infections.
authors: Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, Univ Paris-Diderot, Univ Paris Sud, INRA, Univ Evry, Sorbonne Paris-Cité, Université Paris-Saclay, Bâtiment 630, Gif sur Yvette 91190, France.
authors: Camille
authors: C
authors: Fonouni-Farde
authors: Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, Univ Paris-Diderot, Univ Paris Sud, INRA, Univ Evry, Sorbonne Paris-Cité, Université Paris-Saclay, Bâtiment 630, Gif sur Yvette 91190, France.
authors: Sovanna
authors: S
authors: Tan
authors: Laboratoire des Interactions Plantes-Microorganismes (LIPM), INRA, CNRS, Castanet-Tolosan 31326, France.
authors: Maël
authors: M
authors: Baudin
authors: Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, Univ Paris-Diderot, Univ Paris Sud, INRA, Univ Evry, Sorbonne Paris-Cité, Université Paris-Saclay, Bâtiment 630, Gif sur Yvette 91190, France.
authors: Mathias
authors: M
authors: Brault
authors: Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401, United States of America.
authors: Jiangqi
authors: J
authors: Wen
authors: Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401, United States of America.
authors: Kirankumar S
authors: KS
authors: Mysore
authors: Laboratoire des Interactions Plantes-Microorganismes (LIPM), INRA, CNRS, Castanet-Tolosan 31326, France.
authors: Andreas
authors: A
authors: Niebel
authors: Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, Univ Paris-Diderot, Univ Paris Sud, INRA, Univ Evry, Sorbonne Paris-Cité, Université Paris-Saclay, Bâtiment 630, Gif sur Yvette 91190, France.
authors: Florian
authors: F
authors: Frugier
authors: Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, Univ Paris-Diderot, Univ Paris Sud, INRA, Univ Evry, Sorbonne Paris-Cité, Université Paris-Saclay, Bâtiment 630, Gif sur Yvette 91190, France.
authors: Anouck
authors: A
authors: Diet
mesh_terms: D023081:CCAAT-Binding Factor / Q000378:metabolism; D005875:Gibberellins / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D046913:Medicago truncatula / Q000235:genetics / Q000382:microbiology*; D010940:Plant Proteins / Q000096:biosynthesis / Q000378:metabolism; D055170:Plant Root Nodulation / Q000502:physiology*; D053204:Root Nodules, Plant / Q000382:microbiology*; D015398:Signal Transduction; D016962:Sinorhizobium meliloti / Q000254:growth & development / Q000378:metabolism*
chemical_list: D023081:CCAAT-Binding Factor; C438018:ENOD11 protein, Medicago truncatula; D005875:Gibberellins; D008070:Lipopolysaccharides; D010940:Plant Proteins