title: Monitoring of chlorsulfuron in biological fluids and water samples by molecular fluorescence using rhodamine B as fluorophore.
abstract: A new simple methodology is proposed for chlorsufuron (CS) traces quantification based upon enhancement of rhodamine B (RhB) fluorescent signal. Experimental variables that influence fluorimetric sensitivity have been studied and optimized. The zeroth order regression calibration was linear from 0.866 to 35.800µgL(-1) CS, with a correlation coefficient of 0.99. At optimal experimental conditions, a limit of detection of 0.259µgL(-1) and a limit of quantification of 0.866µgL(-1) were obtained. The method showed good sensitivity and adequate selectivity and was applied to the determination of trace amounts of CS in plasma, serum and water samples with satisfactory results analyzed by ANOVA test. The proposed methodology represents an alternative to traditional chromatographic techniques for CS monitoring in complex samples, using an accessible instrument in control laboratories.
authors: Área de Química Analítica, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina.
authors: Magdalena
authors: M
authors: Alesso
authors: Área de Química Analítica, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina.
authors: Luis A
authors: LA
authors: Escudero
authors: Instituto de Química de San Luis (INQUISAL-CONICET), Chacabuco y Pedernera, San Luis, 5700 Argentina. Electronic address: lfernand@unsl.edu.ar.
authors: María Carolina
authors: MC
authors: Talio
authors: Área de Química Analítica, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina; Instituto de Química de San Luis (INQUISAL-CONICET), Chacabuco y Pedernera, San Luis, 5700 Argentina. Electronic address: lfernand@unsl.edu.ar.
authors: Liliana P
authors: LP
authors: Fernández
mesh_terms: D060766:Drinking Water / Q000032:analysis; D004785:Environmental Pollutants / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D005260:Female; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D060587:Groundwater / Q000032:analysis; D006801:Humans; D008895:Milk, Human / Q000737:chemistry; D010575:Pesticides / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D010949:Plasma / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*; D044967:Serum / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013449:Sulfonamides / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D014227:Triazines / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine
chemical_list: D060766:Drinking Water; D004785:Environmental Pollutants; D005456:Fluorescent Dyes; D010575:Pesticides; D012235:Rhodamines; D013449:Sulfonamides; D014227:Triazines; C029773:rhodamine B; C037137:chlorsulfuron
keywords: Biological and water samples; Chlorsulfuron monitoring; Molecular fluorescence; Rhodamine B