title: Novel bioactive tetracycline-containing electrospun polymer fibers as a potential antibacterial dental implant coating.
abstract: The purpose of this investigation was to determine the ability of tetracycline-containing fibers to inhibit biofilm formation of peri-implantitis-associated pathogens [i.e., Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), Prevotella intermedia (Pi), and Aggregatibacter actinomycetemcomitans (Aa)]. Tetracycline hydrochloride (TCH) was added to a poly(DL-lactide) [PLA], poly(ε-caprolactone) [PCL], and gelatin [GEL] polymer blend solution at distinct concentrations to obtain the following fibers: PLA:PCL/GEL (TCH-free, control), PLA:PCL/GEL + 5 % TCH, PLA:PCL/GEL + 10 % TCH, and PLA:PCL/GEL + 25 % TCH. The inhibitory effect of TCH-containing fibers on biofilm formation was assessed by colony-forming units (CFU/mL). Qualitative analysis of biofilm inhibition was done via scanning electron microscopy (SEM). Statistical significance was reported at p < 0.05. Complete inhibition of biofilm formation on the fibers was observed in groups containing TCH at 10 and 25 wt%. Fibers containing TCH at 5 wt% demonstrated complete inhibition of Aa biofilm. Even though a marked reduction in CFU/mL was observed with an increase in TCH concentration, Pi proved to be the most resilient microorganism. SEM images revealed the absence of or a notable decrease in bacterial biofilm on the TCH-containing nanofibers. Collectively, our data suggest that tetracycline-containing fibers hold great potential as an antibacterial dental implant coating.
authors: Division of Dental Biomaterials, Department of Biomedical and Applied Sciences, Indiana University School of Dentistry (IUSD), 1121 W. Michigan St. (DS270B), Indianapolis, IN, 46202, USA.|Department of Periodontics and Allied Dental Programs, IUSD, Indianapolis, IN, 46202, USA.
authors: R G
authors: RG
authors: Shahi
authors: Division of Dental Biomaterials, Department of Biomedical and Applied Sciences, Indiana University School of Dentistry (IUSD), 1121 W. Michigan St. (DS270B), Indianapolis, IN, 46202, USA.
authors: M T P
authors: MTP
authors: Albuquerque
authors: Division of Dental Biomaterials, Department of Biomedical and Applied Sciences, Indiana University School of Dentistry (IUSD), 1121 W. Michigan St. (DS270B), Indianapolis, IN, 46202, USA.
authors: E A
authors: EA
authors: Münchow
authors: Department of Periodontics and Allied Dental Programs, IUSD, Indianapolis, IN, 46202, USA.
authors: S B
authors: SB
authors: Blanchard
authors: Division of Dental Biomaterials, Department of Biomedical and Applied Sciences, Indiana University School of Dentistry (IUSD), 1121 W. Michigan St. (DS270B), Indianapolis, IN, 46202, USA.
authors: R L
authors: RL
authors: Gregory
authors: Division of Dental Biomaterials, Department of Biomedical and Applied Sciences, Indiana University School of Dentistry (IUSD), 1121 W. Michigan St. (DS270B), Indianapolis, IN, 46202, USA. mbottino@iu.edu.|Department of Biomedical Engineering, Indiana University Purdue University, Indianapolis, IN, 46202, USA. mbottino@iu.edu.|Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, 46202, USA. mbottino@iu.edu.
authors: M C
authors: MC
authors: Bottino
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D015921:Dental Implants; D016967:Fusobacterium nucleatum; D008855:Microscopy, Electron, Scanning; D057873:Peri-Implantitis / Q000382:microbiology* / Q000517:prevention & control*; D011091:Polyesters; D011108:Polymers; D016966:Porphyromonas gingivalis; D018713:Prevotella intermedia; D013234:Stem Cells; D013752:Tetracycline / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; C033616:poly(lactide); D013752:Tetracycline
keywords: Coating; Implant; Nanofibers; Peri-implantitis; Tetracycline