title: Purification of the Yersinia entomophaga Yen-TC Toxin Complex Using Size Exclusion Chromatography.
abstract: The Yersinia entomophaga toxin complex (Yen-TC) is the bacterium's main virulence determinant. Because of its high insect activity, methods were developed to allow the routine isolation and purification of Yen-TC from an overnight bacterial culture using size exclusion chromatography. Here we outline an overnight purification procedure using a 100-ml culture volume, where approximately 2 mg of Yen-TC, with an approximate purity of 95-98 %, can be routinely obtained.
authors: Innovative Farm Systems, AgResearch, Lincoln Research Centre, Canterbury, New Zealand. sandra.jones@agresearch.co.nz.
authors: Sandra A
authors: SA
authors: Jones
authors: Innovative Farm Systems, AgResearch, Lincoln Research Centre, Canterbury, New Zealand.
authors: Mark R H
authors: MR
authors: Hurst
mesh_terms: D001427:Bacterial Toxins / Q000737:chemistry / Q000302:isolation & purification*; D018929:Cell Culture Techniques; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D015007:Yersinia / Q000737:chemistry*
chemical_list: D001427:Bacterial Toxins
keywords: Purification; Size exclusion chromatography; Yen-TC; Yersinia entomophaga