title: The effect of hyperglycemia on blood coagulation: In vitro, observational healthy-volunteer study using rotational thromboelastometry (ROTEM).
abstract: We performed a study to investigate whether contamination of hemostasis samples with a glucose-containing solution might generate spurious results in rotational thromboelastometry (ROTEM) tests.Venous blood was taken from 12 healthy volunteers and divided into 4 specimen bottles, which were contaminated with different concentrations (0%, 5%, 10%, and 20%) of glucose solution.Significant lengthening of INTEMCT was observed in the 10% and 20% groups compared with baseline values (7.7% and 9%, P = 0.041 and P = 0.037, respectively). INTEMCFT increased by 20.1% in the 20% group (P = 0.005). INTEMα-angle and INTEMMCF decreased by 3.9% and 2.7%, respectively, in the 20% group (P = 0.010 and P = 0.049, respectively). EXTEMCFT was prolonged significantly, by 10.2%, 15.5%, and 25.6%, in the 5%, 10%, and 20% groups, respectively (P = 0.004, P < 0.001, and P < 0.001, respectively). EXTEMα-angle decreased significantly by 1.9%, 3.2%, and 4.0% in the 5%, 10%, and 20% groups, respectively (P = 0.014, P = 0.001, and P = 0.005, respectively). EXTEMMCF decreased by 3.4% in the 20% group (P = 0.023). FIBTEMMCF decreased by 9.2% and 17.5% in the 10% and 20% groups, respectively (P = 0.019 and P = 0.021, respectively). A significant correlation was observed between standard glucose solution contamination in the specimens and percentage variation of EXTEMCFT, EXTEMMCF, and FIBTEMMCF.To obtain accurate data from the ROTEM test regarding the hemostatic status of patients, specimens with suspected or known contamination should not be analyzed.
authors: Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seongnam-si Department of Anesthesiology and Pain Medicine, Dankook University Hospital, Cheonan-si, Chungcheongnam-do, South Korea.
authors: Hyun-Jung
authors: HJ
authors: Shin
authors: Hyo-Seok
authors: HS
authors: Na
authors: Soowon
authors: S
authors: Lee
authors: Gwan-Woo
authors: GW
authors: Lee
authors: Sang-Hwan
authors: SH
authors: Do
mesh_terms: D000328:Adult; D001777:Blood Coagulation; D001786:Blood Glucose / Q000032:analysis*; D001800:Blood Specimen Collection / Q000379:methods*; D005440:Fluid Therapy; D005947:Glucose / Q000008:administration & dosage*; D006801:Humans; D066298:In Vitro Techniques; D007261:Infusions, Intra-Arterial; D012996:Solutions; D013916:Thrombelastography / Q000379:methods*
chemical_list: D001786:Blood Glucose; D012996:Solutions; D005947:Glucose