title: PAQR3gene expression and its methylation level in colorectal cancer tissues.
abstract: The aim of the present study was to investigate thePAQR3gene expression and its methylation level in colorectal cancer tissues, as well as the association with colorectal cancer clinical data. In total, 54 cases of colorectal cancer tissue samples and normal adjacent tissue samples were collected between June, 2013 and July, 2014. RT-PCR and western blot analysis were used to detect the mRNA and protein levels of PAQR3 in colorectal samples, respectively. MSP was used to detect the methylation level ofPAQR3gene in colorectal samples, which was compared with colorectal data. The results showed that a decreased expression level of PAQR3 mRNA in colorectal cancer tissues and the expression reduction rate was 57.4% (31/54). Similarly, the expression level of PAQR3 protein was reduced in cancer tissues, and the reduction rate was 46.3% (25/54), while the protein expression reduction rate in cancer adjacent tissue was 5.6% (3/54), and the difference was statistically significant (P<0.05). Furthermore, the methylation rates of PAQR3 in cancer tissues and cancer adjacent tissues were 33.3% (18/54) and 5.6% (3/54), respectively. In addition, PAQR3 mRNA and protein levels in colorectal cancer tissues were associated with the differentiation degree, lymphatic metastasis and tumor infiltration depth. The methylation level of PAQR3 was associated with age, differentiated degree, lymphatic metastasis and tumor infiltration depth. In conclusion, the expression of PAQR3 mRNA and protein in colorectal cancer was reduced and methylation of PAQR3 occurred. Although the PAQR3 mRNA and protein levels were not associated with gender, age or the location of tumor, there was an association with differentiation degree, lymphatic metastasis and tumor infiltration depth. In addition, the methylation level of PAQR3 was not correlated with gender or tumor location, but was correlated with age, differentiation degree, lymphatic metastasis and tumor infiltration depth.
authors: Department of Gastrointestinal Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Ri-Heng
authors: RH
authors: Li
authors: Department of Gastrointestinal Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Ai-Min
authors: AM
authors: Zhang
authors: Department of Blood Transfusion, Neimenggu Xinganleague People's Hospital, Wulanhaote, Inner Mongolia Autonomous Region 137400, P.R. China.
authors: Shuang
authors: S
authors: Li
authors: Clinical Medical College of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Tian-Yang
authors: TY
authors: Li
authors: Clinical Medical College of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Lian-Jing
authors: LJ
authors: Wang
authors: Clinical Medical College of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Hao-Ran
authors: HR
authors: Zhang
authors: Department of Gastrointestinal Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Jian-Wei
authors: JW
authors: Shi
authors: Department of Gastrointestinal Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Xiao-Rui
authors: XR
authors: Liu
authors: Department of Gastrointestinal Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Yuan
authors: Y
authors: Chen
authors: Department of Gastrointestinal Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Ya-Chao
authors: YC
authors: Chen
authors: Department of Gastrointestinal Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Teng-Yao
authors: TY
authors: Wei
authors: Department of Gastrointestinal Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Ying
authors: Y
authors: Gao
authors: Department of Gastrointestinal Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Wei
authors: W
authors: Li
authors: Department of Gastrointestinal Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Hong-Ying
authors: HY
authors: Tang
authors: Department of Gastrointestinal Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China.
authors: Mei-Yu
authors: MY
authors: Tang
keywords: PAQR3; colorectal cancer; mRNA; methylation; western blot analysis