title: Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging.
abstract: We describe a multifocal Raman micro-spectroscopy detection method based on a digital micromirror device, which allows for simultaneous "power-sharing" acquisition of Raman spectra from ad hoc sampling points. As the locations of the points can be rapidly updated in real-time via software control of a liquid-crystal spatial light modulator (LC-SLM), this technique is compatible with automated adaptive- and selective-sampling Raman spectroscopy techniques, the latter of which has previously been demonstrated for fast diagnosis of skin cancer tissue resections. We describe the performance of this instrument and show examples of multiplexed measurements on a range of test samples. Following this, we show the feasibility of reducing measurement time for power-shared multifocal Raman measurements combined with confocal auto-fluorescence imaging to provide guided diagnosis of tumours in human skin samples.
authors: School of Physics and Astronomy, University Park, University of Nottingham, Nottingham, NG7 2RD, UK.
authors: Faris
authors: F
authors: Sinjab
authors: School of Physics and Astronomy, University Park, University of Nottingham, Nottingham, NG7 2RD, UK.
authors: Kenny
authors: K
authors: Kong
authors: School of Physics and Astronomy, University of Glasgow, Kelvin Building, Glasgow G12 8QQ, UK.
authors: Graham
authors: G
authors: Gibson
authors: Circle Nottingham Ltd NHS Treatment Centre, Lister Road, Nottingham NG7 2FT, UK.
authors: Sandeep
authors: S
authors: Varma
authors: Centre of Evidence-Based Dermatology, Nottingham University Hospital NHS Trust, QMC Campus, Derby Road, NG7 2UH, UK.
authors: Hywel
authors: H
authors: Williams
authors: School of Physics and Astronomy, University of Glasgow, Kelvin Building, Glasgow G12 8QQ, UK.
authors: Miles
authors: M
authors: Padgett
authors: School of Physics and Astronomy, University Park, University of Nottingham, Nottingham, NG7 2RD, UK.
authors: Ioan
authors: I
authors: Notingher
keywords: (170.0170) Medical optics and biotechnology; (300.6450) Spectroscopy, Raman