title: Identification of Conidiogenesis-Associated Genes in Colletotrichum gloeosporioides by Agrobacterium tumefaciens-Mediated Transformation.
abstract: The Colletotrichum gloeosporioides is one of the most significant pathogens leading to huge economic losses. To infect plants and cause disease dissemination, the fungus elaborates to produce asexual spores called conidia, which are long-lived and highly resistant to environmental stresses. Here, we report a large-scale, systematic genome-wide screening of conidiogenesis-associated genes via conidiation assays, and high-efficiency TAIL-PCRs. Of 10,210 independent transformants tested, 59 mutants exhibited significant variation in conidial production. The T-DNA right flanking sequences of 11 conidiation-related transformants were further identified, and the obtained sequences were aligned to the genome sequence to uncover the novel loci of sporogenesis. When considering together, this study provided a large number of conidial production-variation mutants and the conidiation-related genes, which will be a valuable resource for characterizing the molecular mechanisms of conidial formation in the fungus.
authors: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, Liaoning, China.
authors: Jianyuan
authors: J
authors: Wu
authors: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, Liaoning, China.
authors: Zhirui
authors: Z
authors: Ji
authors: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, Liaoning, China.
authors: Na
authors: N
authors: Wang
authors: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, Liaoning, China.
authors: Fumei
authors: F
authors: Chi
authors: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, Liaoning, China.
authors: Chengnan
authors: C
authors: Xu
authors: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, Liaoning, China.
authors: Zongshan
authors: Z
authors: Zhou
authors: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, Liaoning, China. zhangjunxiang@caas.cn.
authors: Junxiang
authors: J
authors: Zhang
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000502:physiology; D020231:Colletotrichum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics* / Q000502:physiology; D016254:Mutagenesis, Insertional; D010935:Plant Diseases / Q000382:microbiology; D013172:Spores, Fungal / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D005656:Fungal Proteins