title: Measuring Vascular Permeability In Vivo.
abstract: Over the past decades, in vivo vascular permeability measurements have provided significant insight into vascular functions in physiological and pathophysiological conditions such as the response to pro- and anti-angiogenic signaling, abnormality of tumor vasculature and its normalization, and delivery and efficacy of therapeutic agents. Different approaches for vascular permeability measurements have been established. Here, we describe and discuss a conventional 2D imaging method to measure vascular permeability, which was originally documented by Gerlowski and Jain in 1986 (Microvasc Res 31:288-305, 1986) and further developed by Yuan et al. in the early 1990s (Microvasc Res 45:269-289, 1993; Cancer Res 54:352-3356, 1994), and our recently developed 3D imaging method, which advances the approach originally described by Brown et al. in 2001 (Nat Med 7:864-868, 2001).
authors: Edwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, 100 Blossom Street, Boston, MA, 02114, USA.
authors: Eelco F J
authors: EF
authors: Meijer
authors: Edwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, 100 Blossom Street, Boston, MA, 02114, USA.|Department of Mechanical and Biomedical Engineering, Bucknell University, Lewisburg, PA, 17837, USA.
authors: James W
authors: JW
authors: Baish
authors: Edwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, 100 Blossom Street, Boston, MA, 02114, USA.
authors: Timothy P
authors: TP
authors: Padera
authors: Edwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, 100 Blossom Street, Boston, MA, 02114, USA. dai@steele.mgh.harvard.edu.
authors: Dai
authors: D
authors: Fukumura
mesh_terms: D000818:Animals; D002199:Capillary Permeability; D006801:Humans; D021621:Imaging, Three-Dimensional; D066298:In Vitro Techniques; D000069416:Intravital Microscopy / Q000379:methods; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods
keywords: Intravital fluorescence microscopy; Multiphoton microscopy; Vascular normalization; Vascular permeability