title: Ethambutol induces testicular damage and decreases the sperm functional competence in Swiss albino mice.
abstract: The present study reports the effect of ethambutol (EMB) on testicular function. Prepubertal and adult male Swiss albino mice were treated with 40, 80, 160mg/kg body weight of EMB, intraperitoneally, every alternate day for 4 weeks. After 2 weeks gap, mice were sacrificed to collect caudal spermatozoa. EMB treatment resulted in a dose-dependent decrease in the testicular weight, sperm count and motility while the percentage of sperm with head abnormalities, immature chromatin (P<0.001) and DNA damage increased (P<0.01). In addition, EMB treatment resulted in significant depletion of glutathione (P<0.05-P<0.01) and histopathological abnormalities such as large cells, vacuolation of tubules and isolated colonies of spermatogenic cells were observed. Oct4, 17β-Hsd and c-Kit mRNA was marginally elevated in EMB treated testes at the highest dose studied. In conclusion, the result of the present study indicates that EMB has adverse effect on testicular function and impairs the sperm functional competence.
authors: Department of Clinical Embryology, Level 2, Central Research Lab., Manipal 576104, Karnataka, India.
authors: Arpitha
authors: A
authors: Rao
authors: Department of Clinical Embryology, Level 2, Central Research Lab., Manipal 576104, Karnataka, India.
authors: Guruprasad
authors: G
authors: Nayak
authors: Department of Clinical Embryology, Level 2, Central Research Lab., Manipal 576104, Karnataka, India.
authors: Sandhya
authors: S
authors: Kumari
authors: Department of Clinical Embryology, Level 2, Central Research Lab., Manipal 576104, Karnataka, India.
authors: Amratha D
authors: AD
authors: Prabhu
authors: Department of Biochemistry, Manipal 576104, Karnataka, India.
authors: Nalini
authors: N
authors: Khandige
authors: Department of Anatomy, Kasturba Medical College, Manipal 576104, Karnataka, India.
authors: Sneha Guruprasad
authors: SG
authors: Kalthur
authors: Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, Karnataka, India.
authors: Srinivas
authors: S
authors: Mutalik
authors: Department of Clinical Embryology, Level 2, Central Research Lab., Manipal 576104, Karnataka, India. Electronic address: guru.kalthur@manipal.edu.
authors: Guruprasad
authors: G
authors: Kalthur
authors: Department of Clinical Embryology, Level 2, Central Research Lab., Manipal 576104, Karnataka, India.
authors: Satish Kumar
authors: SK
authors: Adiga
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D002843:Chromatin / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004977:Ethambutol / Q000009:adverse effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D051336:Mitochondrial Membranes / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D013739:Testosterone / Q000378:metabolism
chemical_list: D002843:Chromatin; D013739:Testosterone; D004977:Ethambutol; D005978:Glutathione
keywords: Ethambutol; GSH; Sperm DNA damage; Testicular toxicity; Testosterone