title: Karyopherinβ1 regulates proliferation of human glioma cells via Wnt/β-catenin pathway.
abstract: Karyopherinβ1 (KPNB1), one of the cytosolic factors involved in the selective protein transport across nucleus, docked at nuclear pore complex and transported through nuclear envelope in an ATP-dependent style, assisting proteins to be recognized as import substrates. It has been reported to be bound up with the origination and progress of lung cancer, cervical cancer, head and neck cancer and hepatocellular carcinoma. In current study, we demonstrated for the first time that the role of KPNB1 in human glioma. KPNB1 was over-expressed as the well-known trend of Ki-67(p < 0.01) and tightly closed to poor prognosis, as an independent prognostic factor. In vitro, up-regulation of KPNB1 was accompanied by certain rising levels of proliferation markers, employing U251 and U87MG cells as serum-starve models. Silencing KPNB1 in U251 and U87MG led to G1 phase arrested directly via flow cytometry analysis. In the nucleus of KPNB1-depletion cell models, the decreasing expression of KPNB1 and β-catenin was detected respectively, which indicated that KPNB1 functioned via β-catenin signal. Besides, the interaction between KPNB1 and β-catenin was proved clearly by immunoprecipitation. Taken together, it showed that KPNB1 might enhance human glioma proliferation via Wnt/β-Catenin Pathway.
authors: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors: Ting
authors: T
authors: Lu
authors: Department of Neurosurgery, The Affiliated Dushuhu Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors: Zhen
authors: Z
authors: Bao
authors: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors: Yunfeng
authors: Y
authors: Wang
authors: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors: Lixiang
authors: L
authors: Yang
authors: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors: Bing
authors: B
authors: Lu
authors: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors: Ke
authors: K
authors: Yan
authors: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors: Shaozhen
authors: S
authors: Wang
authors: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China.
authors: He
authors: H
authors: Wei
authors: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, People's Republic of China.
authors: Zhe
authors: Z
authors: Zhang
authors: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People's Republic of China. Electronic address: cuigang120@sina.com.
authors: Gang
authors: G
authors: Cui
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D011379:Prognosis; D011485:Protein Binding; D021381:Protein Transport; D016019:Survival Analysis; D060449:Wnt Signaling Pathway; D014944:World Health Organization; D051176:beta Catenin / Q000378:metabolism; D028961:beta Karyopherins / Q000378:metabolism*
chemical_list: C424166:KPNB1 protein, human; D019394:Ki-67 Antigen; D051176:beta Catenin; D028961:beta Karyopherins
keywords: Human glioma; KPNB1; Proliferation; Wnt/β-Catenin