title: Propofol inhibits carbachol-induced chloride secretion by directly targeting the basolateral K+channel in rat ileum epithelium.
abstract: BACKGROUND
  Propofol is a widely used intravenous general anesthetic. Acetylcholine (ACh) is critical in controlling epithelial ion transport. This study was to investigate the effects of propofol on ACh-evoked secretion in rat ileum epithelium.
  
  
  METHODS
  The Ussing chamber technique was used to investigate the effects of propofol on carbachol (CCh)-evoked short-circuit currents (Isc).
  
  
  RESULTS
  Propofol (10-2-10-6mol/L) attenuated CCh-evoked Isc of rat ileum mucosa in a dose-dependent manner. The inhibitory effect of propofol was only evident after application to the serosal side. Pretreatment with tetrodotoxin (TTX, 0.3 μmol/L, n=5) had no effect on propofol-induced inhibitory effect, whereas serosal application of K+channel inhibitor, glibenclamide, but not, an ATP-sensitive K+channel inhibitor, largely reduced the inhibitory effect of propofol. In addition, pretreatment with either hexamethonium bromide (HB, nicotinic nACh receptor antagonist) or Cl-channel blockers niflumic acid and cystic fibrosis transmembrane conductance regulator (inh)-172 did not produce any effect on the propofol-induced inhibitory effect.
  
  
  CONCLUSIONS
  Propofol inhibits CCh-induced intestinal secretion by directly targeting basolateral K+channels.
authors: Department of Anesthesiology, Qilu Hospital, Shandong University, Jinan, China.
authors: S-H
authors: SH
authors: Tang
authors: Department of Physiology, School of Medicine, Shandong University, Jinan, China.
authors: H-Y
authors: HY
authors: Wang
authors: Department of Thoracic Surgery, Shandong Tumor Hospital, Shandong University, Jinan, China.
authors: H
authors: H
authors: Sun
authors: Department of Physiology, School of Medicine, Shandong University, Jinan, China.
authors: N
authors: N
authors: An
authors: Department of Physiology, School of Medicine, Shandong University, Jinan, China.
authors: L
authors: L
authors: Xiao
authors: Department of Physiology, School of Medicine, Shandong University, Jinan, China.
authors: Q
authors: Q
authors: Sun
authors: Department of Thoracic Surgery, Shandong Tumor Hospital, Shandong University, Jinan, China.
authors: D-B
authors: DB
authors: Zhao
mesh_terms: D018686:Anesthetics, Intravenous / Q000494:pharmacology; D000818:Animals; D002217:Carbachol / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002712:Chlorides / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods; D007082:Ileum / Q000187:drug effects / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D015221:Potassium Channels / Q000502:physiology; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018686:Anesthetics, Intravenous; D002712:Chlorides; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D002217:Carbachol; D015742:Propofol
keywords: K+ channels; acetylcholine receptor; intestinal secretion; propofol; short-circuit current