title: Identification of candidate miRNAs and expression profile of yak oocytes before and after in vitro maturation by high-throughput sequencing.
abstract: Small RNA represents several unique non-coding RNA classes that have important function in a wide range of biological processes including development of germ cells and early embryonic, cell differentiation, cell proliferation and apoptosis in diverse organisms. However, little is known about their expression profiles and effects in yak oocytes maturation and early development. To investigate the function of small RNAs in the maturation process of yak oocyte and early development, two small RNA libraries of oocytes were constructed from germinal vesicle stage (GV) and maturation in vitro to metaphase II-arrested stage (M II) and then sequenced using small RNA high-throughput sequencing technology. A total of 9,742,592 and 12,168,523 clean reads were obtained from GV and M II oocytes, respectively. In total, 801 and 1,018 known miRNAs were acquired from GV and M II oocytes, and 75 miRNAs were found to be significantly differentially expressed: 47 miRNAs were upregulated and 28 miRNAs were downregulated in the M II oocytes compared to the GV stage. Among the upregulated miRNAs, miR-342 has the largest fold change (9.25-fold). Six highly expressed miRNAs (let-7i, miR-10b, miR-10c, miR-143, miR-146b and miR-148) were validated by real-time quantitative PCR (RT-qPCR) and consistent with the sequencing results. Furthermore, the expression patterns of two miRNAs and their potential targets were analysed in different developmental stages of oocytes and early embryos. This study provides the first miRNA profile in the mature process of yak oocyte. Seventy-five miRNAs are expressed differentially in GV and M II oocytes as well as among different development stages of early embryos, suggesting miRNAs involved in regulating oocyte maturation and early development of yak. These results showed specific miRNAs in yak oocytes had dynamic changes during meiosis. Further functional and mechanistic studies on the miRNAs during meiosis may beneficial to understanding the role of miRNAs on meiotic division.
authors: College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.|College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, China.
authors: X R
authors: XR
authors: Xiong
authors: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, China.
authors: D L
authors: DL
authors: Lan
authors: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, China.
authors: J
authors: J
authors: Li
authors: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, China.
authors: X D
authors: XD
authors: Zi
authors: College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
authors: M Y
authors: MY
authors: Li
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D059014:High-Throughput Nucleotide Sequencing / Q000662:veterinary*; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D059467:Transcriptome / Q000502:physiology*
chemical_list: D035683:MicroRNAs