title: Microbial phylogenetic and functional responses within acidified wastewater communities exhibiting enhanced phosphate uptake.
abstract: Acid stimulated accumulation of insoluble phosphorus within microbial cells is highly beneficial to wastewater treatment but remains largely unexplored. Using single cell analyses and next generation sequencing, the response of active polyphosphate accumulating microbial communities under conditions of enhanced phosphorus uptake under both acidic and aerobic conditions was characterised. Phosphorus accumulation activities were highest under acidic conditions (pH 5.5>8.5), where a significant positive effect on bioaccumulation was observed at pH 5.5 when compared to pH 8.5. In contrast to the Betaproteobacteria and Actinobacteria dominated enhanced biological phosphorus removal process, the functionally active polyP accumulators at pH 5.5 belonged to the Gammaproteobacteria, with key accumulators identified as members of the families Aeromonadaceae and Enterobacteriaceae. This study demonstrated a significant enrichment of key polyphosphate kinase and exopolyphosphatase genes within the community metagenome after acidification, concomitant with an increase in P accumulation kinetics.
authors: School of Earth and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia; Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia.
authors: Anjani W
authors: AW
authors: Weerasekara
authors: School of Earth and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia; Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia.
authors: Sasha
authors: S
authors: Jenkins
authors: School of Earth and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia; Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia.
authors: Lynette K
authors: LK
authors: Abbott
authors: School of Earth and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia; Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia.
authors: Ian
authors: I
authors: Waite
authors: School of Biological Sciences and the Institute for Global Food Security, The Queens University of Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, United Kingdom.
authors: John W
authors: JW
authors: McGrath
authors: Centre for Microscopy, Characterisation and Analysis (CMCA), The University of Western Australia, Crawley, 35 Stirling Highway, 6009 Western Australia, Australia.
authors: Irma
authors: I
authors: Larma
authors: Department of Chemical Engineering, Curtin University, Perth 6845, Western Australia, Australia.
authors: Ela
authors: E
authors: Eroglu
authors: School of Earth and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia; Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia.
authors: Anthony
authors: A
authors: O'Donnell
authors: School of Earth and Environment, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia. Electronic address: andy.whiteley@uwa.edu.au.
authors: Andrew S
authors: AS
authors: Whiteley
mesh_terms: D020562:Betaproteobacteria / Q000235:genetics; D020563:Gammaproteobacteria / Q000235:genetics / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D010758:Phosphorus / Q000378:metabolism; D010802:Phylogeny; D011122:Polyphosphates / Q000378:metabolism*; D061005:Ponds; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology*; D014904:Western Australia
chemical_list: D010758:Phosphorus; D011122:Polyphosphates; D062065:Wastewater
keywords: Acid-stimulated biological phosphorus removal; Polyphosphate accumulating organisms; Polyphosphate kinase; pH