title: Yeast factories for the production of aromatic compounds: from building blocks to plant secondary metabolites.
abstract: The aromatic amino acid biosynthesis pathway is a source to a plethora of commercially relevant chemicals with very diverse industrial applications. Tremendous efforts in microbial engineering have led to the production of compounds ranging from small aromatic molecular building blocks all the way to intricate plant secondary metabolites. Particularly, the yeast Saccharomyces cerevisiae has been a great model organism given its superior capability to heterologously express long metabolic pathways, especially the ones containing cytochrome P450 enzymes. This review contains a collection of state-of-the-art metabolic engineering work devoted towards unraveling the mechanisms for enhancing the flux of carbon into the aromatic pathway. Some of the molecules discussed include the polymer precursor muconic acid, as well as important nutraceuticals (flavonoids and stilbenoids), and opium-derived drugs (benzylisoquinoline alkaloids).
authors: Department of Chemical and Biological Engineering, Iowa State University, Ames, IA, 50010, USA.|NSF Engineering Research Center for Biorenewable Chemicals, Iowa State University, Ames, IA, 50010, USA.
authors: Miguel
authors: M
authors: Suástegui
authors: Department of Chemical and Biological Engineering, Iowa State University, Ames, IA, 50010, USA. zyshao@iastate.edu.|Microbiology Interdisciplinary Program, Iowa State University, Ames, IA, 50010, USA. zyshao@iastate.edu.|NSF Engineering Research Center for Biorenewable Chemicals, Iowa State University, Ames, IA, 50010, USA. zyshao@iastate.edu.
authors: Zengyi
authors: 0000-0001-6817-8006
authors: Z
authors: Shao
mesh_terms: D024322:Amino Acids, Aromatic / Q000096:biosynthesis*; D044182:Benzylisoquinolines / Q000378:metabolism; D053898:Biosynthetic Pathways; D019587:Dietary Supplements; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways; D010944:Plants / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D064210:Secondary Metabolism; D012765:Shikimic Acid / Q000378:metabolism; D013011:Sorbic Acid / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D024322:Amino Acids, Aromatic; D044182:Benzylisoquinolines; D012765:Shikimic Acid; C005939:muconic acid; D013011:Sorbic Acid
keywords: Aromatic amino acids; Benzylisoquinoline alkaloids; Flavonoids; Metabolic engineering; Saccharomyces cerevisiae; Shikimic acid; Stilbenoids