title: A magnetically actuated cellular strain assessment tool for quantitative analysis of strain induced cellular reorientation and actin alignment.
abstract: Commercially available cell strain tools, such as pneumatically actuated elastomer substrates, require special culture plates, pumps, and incubator setups. In this work, we present a magnetically actuated cellular strain assessment tool (MACSAT) that can be implemented using off-the-shelf components and conventional incubators. We determine the strain field on the MACSAT elastomer substrate using numerical models and experimental measurements and show that a specific region of the elastomer substrate undergoes a quasi-uniaxial 2D stretch, and that cells confined to this region of the MACSAT elastomer substrate undergo tensile, compressive, or zero axial strain depending on their angle of orientation. Using the MACSAT to apply cyclic strain on endothelial cells, we demonstrate that actin filaments within the cells reorient away from the stretching direction, towards the directions of minimum axial strain. We show that the final actin orientation angles in strained cells are spread over a region of compressive axial strain, confirming previous findings on the existence of a varied pre-tension in the actin filaments of the cytoskeleton. We also demonstrate that strained cells exhibit distinctly different values of actin alignment coherency compared to unstrained cells and therefore propose that this parameter, i.e., the coherency of actin alignment, can be used as a new readout to determine the occurrence/extent of actin alignment in cell strain experiments. The tools and methods demonstrated in this study are simple and accessible and can be easily replicated by other researchers to study the strain response of other adherent cells.
authors: Department of Mechanical Engineering, University of British Columbia, Vancouver, British Columbia V6T-1Z4, Canada.
authors: F
authors: F
authors: Khademolhosseini
authors: Department of Medicine, University of British Columbia, Vancouver, British Columbia V5Z-1M9, Canada.
authors: C-C
authors: CC
authors: Liu
authors: Child and Family Research Institute, BC Children's Hospital, Vancouver, British Columbia V5Z-4H4, Canada.
authors: C J
authors: CJ
authors: Lim
authors: Department of Mechanical Engineering, University of British Columbia, Vancouver, British Columbia V6T-1Z4, Canada.
authors: M
authors: M
authors: Chiao
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D013314:Stress, Mechanical
chemical_list: D000199:Actins