title: High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster.
abstract: The consumption of a high-fat diet (HFD) causes alteration in normal metabolism affecting lifespan of flies; however molecular mechanism associated with this damage in flies is not well known. This study evaluates the effects of ingestion of a diet supplemented with 10% and 20% of coconut oil, which is rich in saturated fatty acids, on oxidative stress and cells stress signaling pathways. After exposure to the diet for seven days, cellular and mitochondrial viability, lipid peroxidation and antioxidant enzymes SOD and CAT activity, and mRNA expression of antioxidant enzymes HSP83 and MPK2 were analyzed. To confirm the damage effect of diet on flies, survival and lifespan were investigated. The results revealed that the HFD augmented the rate of lipid peroxidation and SOD and CAT activity and induced a higher expression of HSP83 and MPK2 mRNA. In parallel, levels of enzymes involved in lipid metabolism (ACSL1 and ACeCS1) were increased. Our data demonstrate that association among metabolic changes, oxidative stress, and protein signalization might be involved in shortening the lifespan of flies fed with a HFD.
authors: Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas, Universidade Federal do Pampa (Unipampa), Campus Itaqui, 97650-000 Itaqui, RS, Brazil.
authors: Mariane
authors: M
authors: Trindade de Paula
authors: Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas, Universidade Federal do Pampa (Unipampa), Campus Itaqui, 97650-000 Itaqui, RS, Brazil.
authors: Márcia Rósula
authors: MR
authors: Poetini Silva
authors: Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas, Universidade Federal do Pampa (Unipampa), Campus Itaqui, 97650-000 Itaqui, RS, Brazil.
authors: Stífani
authors: S
authors: Machado Araujo
authors: Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas, Universidade Federal do Pampa (Unipampa), Campus Itaqui, 97650-000 Itaqui, RS, Brazil.
authors: Vandreza
authors: V
authors: Cardoso Bortolotto
authors: Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas, Universidade Federal do Pampa (Unipampa), Campus Itaqui, 97650-000 Itaqui, RS, Brazil.
authors: Luana
authors: L
authors: Barreto Meichtry
authors: Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors: Ana Paula Pegoraro
authors: AP
authors: Zemolin
authors: Centro Interdisciplinar de Pesquisa em Biotecnologia (CIP/BIOTEC), Universidade Federal do Pampa, Campus São Gabriel, 97300 000 São Gabriel, RS, Brazil.
authors: Gabriel L
authors: GL
authors: Wallau
authors: Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas, Universidade Federal do Pampa (Unipampa), Campus Itaqui, 97650-000 Itaqui, RS, Brazil.
authors: Cristiano Ricardo
authors: CR
authors: Jesse
authors: Centro Interdisciplinar de Pesquisa em Biotecnologia (CIP/BIOTEC), Universidade Federal do Pampa, Campus São Gabriel, 97300 000 São Gabriel, RS, Brazil.
authors: Jeferson Luís
authors: 0000-0002-3874-8618
authors: JL
authors: Franco
authors: Centro Interdisciplinar de Pesquisa em Biotecnologia (CIP/BIOTEC), Universidade Federal do Pampa, Campus São Gabriel, 97300 000 São Gabriel, RS, Brazil.
authors: Thaís
authors: T
authors: Posser
authors: Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas, Universidade Federal do Pampa (Unipampa), Campus Itaqui, 97650-000 Itaqui, RS, Brazil.
authors: Marina
authors: 0000-0002-9724-7469
authors: M
authors: Prigol
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D000074263:Coconut Oil; D059305:Diet, High-Fat; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D008136:Longevity / Q000187:drug effects / Q000235:genetics; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D009043:Motor Activity / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D015996:Survival Rate; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D001786:Blood Glucose; D029721:Drosophila Proteins; D006360:Heat-Shock Proteins; C089272:Hsp83 protein, Drosophila; D010938:Plant Oils; D014280:Triglycerides; D020928:Mitogen-Activated Protein Kinases; D000110:Acetylcholinesterase; D000074263:Coconut Oil