title: Immunization with electroporation enhances the protective effect of a DNA vaccine candidate expressing prME antigen against dengue virus serotype 2 infection.
abstract: OBJECTIVE
  We aimed to use the dengue virus (DV) serotype 2 as a proof of principal for testing the efficacy of a DNA vaccine candidate via in vivo electroporation in mice and rabbits prior to the development of a tetravalent vaccine.
  
  
  METHODS
  Different dosages of DNA pVAX1-D2ME encoding DV2 prME genes were vaccinated in mice via intramuscular injection or in vivo electroporation, immune responses and protection were determined. In DNA-vaccinated rabbits via electroporation, antibody titer and protein expression were tested.
  
  
  RESULTS
  In mice, 50μg of pVAX1-D2ME via electroporation elicited effective anti-DV2 responses and conferred significant protection against DV2 challenge. Moreover, anti-DV2 IgG and neutralizing antibodies were successfully induced in rabbits immunized with pVAX1-D2ME via electroporation and the expression of the interest protein was observed at local sites.
  
  
  CONCLUSIONS
  Enhanced immunogenicity and protective effect conferred by pVAX1-D2ME via electroporation show great promise for the development of a dengue tetravalent DNA vaccine.
authors: Department of Microbiology and Parasitology, School of Basic Medical Sciences, Chinese Capital Medical University, Beijing 100069, PR China.
authors: Hui
authors: H
authors: Chen
authors: Department of Microbiology and Parasitology, School of Basic Medical Sciences, Chinese Capital Medical University, Beijing 100069, PR China.
authors: Xiaoyan
authors: X
authors: Zheng
authors: Department of Microbiology and Parasitology, School of Basic Medical Sciences, Chinese Capital Medical University, Beijing 100069, PR China.
authors: Ran
authors: R
authors: Wang
authors: Department of Microbiology and Parasitology, School of Basic Medical Sciences, Chinese Capital Medical University, Beijing 100069, PR China.
authors: Na
authors: N
authors: Gao
authors: Department of Microbiology and Parasitology, School of Basic Medical Sciences, Chinese Capital Medical University, Beijing 100069, PR China.
authors: Ziyang
authors: Z
authors: Sheng
authors: Department of Microbiology and Parasitology, School of Basic Medical Sciences, Chinese Capital Medical University, Beijing 100069, PR China.
authors: Dongying
authors: D
authors: Fan
authors: Department of Microbiology and Parasitology, School of Basic Medical Sciences, Chinese Capital Medical University, Beijing 100069, PR China.
authors: Kaihao
authors: K
authors: Feng
authors: Department of Microbiology and Parasitology, School of Basic Medical Sciences, Chinese Capital Medical University, Beijing 100069, PR China.
authors: Xianzheng
authors: X
authors: Liao
authors: Department of Microbiology and Parasitology, School of Basic Medical Sciences, Chinese Capital Medical University, Beijing 100069, PR China; Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing 100069, PR China. Electronic address: anjing60@aliyun.com.
authors: Jing
authors: J
authors: An
mesh_terms: D000330:Aedes; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D016207:Cytokines / Q000276:immunology; D003715:Dengue / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D053059:Dengue Vaccines / Q000008:administration & dosage*; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D018274:Electroporation; D005260:Female; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D018482:Muscle, Skeletal / Q000276:immunology; D011817:Rabbits; D013154:Spleen / Q000166:cytology; D019444:Vaccines, DNA / Q000008:administration & dosage*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D016207:Cytokines; D053059:Dengue Vaccines; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D014764:Viral Proteins
keywords: DNA vaccine; Dengue virus; Electroporation; Immunization; Vaccine