title: The Use of Chick Embryos to Study Wnt Activity Gradients.
abstract: The chick spinal cord provides a valuable model for assessing Wnt signaling activity. Loss or gain of function constructs that are transfected by electroporation can be directed to a single side of the spinal cord, thus leaving the contralateral side as an internal control. Here, we describe a method for measuring Wnt signaling via the use of BAT-Gal, a β-catenin dependent Wnt reporter.
authors: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA, 94132, USA.
authors: Lisa M
authors: LM
authors: Galli
authors: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA, 94132, USA.
authors: Tiffany
authors: T
authors: Barnes
authors: Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA, 94132, USA. LBurrus@sfsu.edu.
authors: Laura W
authors: LW
authors: Burrus
mesh_terms: D000818:Animals; D002642:Chick Embryo; D008967:Molecular Biology / Q000379:methods*; D013116:Spinal Cord / Q000378:metabolism; D051153:Wnt Proteins / Q000235:genetics*; D060449:Wnt Signaling Pathway / Q000235:genetics; D051176:beta Catenin / Q000235:genetics*
chemical_list: D051153:Wnt Proteins; D051176:beta Catenin
keywords: BAT-Gal; Chick; Electroporation; Gradient; In ovo; Spinal cord; WNT1; WNT3A; β-catenin