title: Hydrogen peroxide derived from marine peroxy sesquiterpenoids induces apoptosis in HCT116 human colon cancer cells.
abstract: In this study, the isolates of the peroxy sesquiterpenoids (1-3) from the Okinawan soft coral, Sinularia sp., indicated cytotoxicity in HCT116 colon cancer cells. The apoptotic cells with a nuclear condensation were detected in the presence of these compounds, then the caspase 3/7 activity was induced, indicating that the compounds have a potential antitumor activity by apoptosis-induction. The cells treated with these compounds were generated reactive oxygen species (ROS), indicating that the ROS is related to the induction of apoptosis. The ROS production reduced in the presence of catalase or trolox, indicating that hydrogen peroxide (H2O2) is generated through a certain free radical reaction derived from the compound. In fact, the accumulation of intracellular H2O2 was also confirmed in the presence of these compounds. Based on all the results, this study proposed the apoptosis-inducing mechanism due to the compounds that the H2O2 produced involving free radical reactions derived from cleavage of the end or hydro-peroxide in the molecule induced cell death.
authors: Department of Bioresource Technology, Okinawa National College of Technology, 905 Henoko, Nago-shi, Okinawa 905-2192, Japan.
authors: Haruna
authors: H
authors: Miyazato
authors: Department of Bioresource Technology, Okinawa National College of Technology, 905 Henoko, Nago-shi, Okinawa 905-2192, Japan. Electronic address: taira@okinawa-ct.ac.jp.
authors: Junsei
authors: J
authors: Taira
authors: Department of Chemistry, Biology and Marine Science, University of the Ryukyus, 1 Senbaru, Nishihara-cho, Okinawa 903-2013, Japan.
authors: Katsuhiro
authors: K
authors: Ueda
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D045325:HCT116 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012717:Sesquiterpenes / Q000494:pharmacology*
chemical_list: D017382:Reactive Oxygen Species; D012717:Sesquiterpenes; D006861:Hydrogen Peroxide
keywords: Apoptosis; HCT colon cancer cells; Hydrogen peroxide; Peroxy sesquiterpenoid; Reactive oxygen species