title: MiR-30a-5p Suppresses Tumor Metastasis of Human Colorectal Cancer by Targeting ITGB3.
abstract: OBJECTIVE
  MicroRNAs (miRNAs) are dysregulated in a wide range of malignant diseases, confirming their crucial role in tumor metastasis. MiRNA-30a-5p, a member of the miR-30 family, has been implicated in many types of cancers, including colorectal cancer, a leading cause of death worldwide.
  
  
  METHODS
  qRT-PCR, Western blot, Transwell assay,luciferase reporter assay were performed in the present study.
  
  
  RESULTS
  In this study, miR-30a-5p was found to be significantly downregulated in human colorectal cancer tissue specimens and cell lines compared with non-cancerous tissues and cells. The overexpression of miR-30a-5p inhibited the migratory and invasive abilities of colorectal cancer cells and suppressed the epithelial-mesenchymal transition, a crucial process in metastasis. Bioinformatic algorithms and luciferase reporter assays revealed that integrin β3 (ITGB3) is a direct target of miR-30a-5p. Importantly, overexpression of ITGB3 in colorectal cancer cells rescued these cells from miR-30a-5p-mediated suppression of metastasis and restored the epithelial-mesenchymal transition.
  
  
  CONCLUSIONS
  Taken together, our study provides the first evidence that miR-30a-5p suppresses colon cancer metastasis through the inhibition of ITGB3. Thus, targeting miR-30a-5p might serve as a promising therapeutic strategy for the treatment of colorectal cancer.
authors: Department of Anorectal, the Central Hospital of Xinxiang, Xinxiang, China.
authors: Wei
authors: W
authors: Wei
authors: Yang
authors: Y
authors: Yang
authors: Jian
authors: J
authors: Cai
authors: Kai
authors: K
authors: Cui
authors: Rong Xian
authors: RX
authors: Li
authors: Huan
authors: H
authors: Wang
authors: Xiujuan
authors: X
authors: Shang
authors: Dong
authors: D
authors: Wei
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D039661:Integrin beta3 / Q000235:genetics* / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D015398:Signal Transduction; D013160:Splenic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: C061248:ITGB3 protein, human; D039661:Integrin beta3; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D008156:Luciferases