title: A virus-MIPs fluorescent sensor based on FRET for highly sensitive detection of JEV.
abstract: Major stumbling blocks in the recognition and detection of virus are the unstable biological recognition element or the complex detection means. Here a fluorescent sensor based on virus-molecular imprinted polymers (virus-MIPs) was designed for specific recognition and highly sensitive detection of Japanese encephalitis virus (JEV). The virus-MIPs were anchored on the surface of silica microspheres modified by fluorescent dye, pyrene-1-carboxaldehyde (PC). The fluorescence intensity of PC can be enhanced by the principle of fluorescence resonance energy transfer (FRET), where virus acted as energy donor and PC acted as energy acceptor. The enhanced fluorescence intensity was proportional to the concentration of virus in the range of 24-960pM, with a limit of detection (LOD, 3σ) of 9.6pM, and the relative standard deviation was 1.99%. In additional, the specificity study confirmed the resultant MIPs has high-selectivity for JEV. This sensor would become a new key for the detection of virus because of its high sensitive, simple operation, high stability and low cost.
authors: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan, 411105 China.
authors: Caishuang
authors: C
authors: Liang
authors: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan, 411105 China.
authors: Huan
authors: H
authors: Wang
authors: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan, 411105 China.
authors: Kui
authors: K
authors: He
authors: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan, 411105 China.
authors: Chunyan
authors: C
authors: Chen
authors: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan, 411105 China.
authors: Xiaoming
authors: X
authors: Chen
authors: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan, 411105 China.
authors: Hang
authors: H
authors: Gong
authors: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan, 411105 China. Electronic address: cai_mao3@hotmail.com.
authors: Changqun
authors: C
authors: Cai
mesh_terms: D015374:Biosensing Techniques; D004664:Encephalitis Virus, Japanese / Q000648:ultrastructure; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D054802:Molecular Imprinting; D044967:Serum / Q000821:virology; D012822:Silicon Dioxide / Q000737:chemistry; D014873:Water Pollutants / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D014873:Water Pollutants; D012822:Silicon Dioxide
keywords: Fluorescence resonance energy transfer (fret); Fluorescent sensor; Japanese encephalitis virus (jev); Pyrene-1-carboxaldehyde (pc); Virus-molecular imprinted polymers (virus-mips)