title: Polycomb-group protein SlMSI1 represses the expression of fruit-ripening genes to prolong shelf life in tomato.
abstract: Polycomb-group (PcG) protein MULTICOPY SUPPRESSOR OF IRA1 (MSI1) protein is an evolutionarily conserved developmental suppressor and plays a crucial role in regulating epigenetic modulations. However, the potential role and function of MSI1 in fleshy fruits remain unknown. In this study, SlMSI1 was cloned and transformed into tomato to explore its function. The quantitative real-time PCR results showed that SlMSI1 was highly expressed in flowers and fruits and that its transcript and protein levels were significantly decreased in fruits after the breaker stage. Additionally, SlMSI1-overexpressing transgenic tomatoes displayed abnormal non-ripening fruit formation, whereas its suppression promoted fruit ripening in transgenic tomatoes. Quantitative real-time PCR assays also showed that RIN and its regulons were decreased in SlMSI1 overexpression transgenic tomato fruits. Furthermore, RNA-seq analysis demonstrated that SlMSI1 inhibits fruit ripening by negatively regulating a large set of fruit-ripening genes in addition to RIN and its regulons. Finally, genetic manipulation of SlMSI1 and RIN successfully prolonged the fruit shelf life by regulating the fruit-ripening genes in tomato. Our findings reveal a novel regulatory function of SlMSI1 in fruit ripening and provide a new regulator that may be useful for genetic engineering and modification of fruit shelf life.
authors: National Key Laboratory of Crop Biology, Shandong Agricultural University, Tai-An, Shandong 271018, China.|College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong 271018, China.|College of Agriculture, Yunnan University, Kunming, Yunnan 650091, China.
authors: Dan-Dan
authors: DD
authors: Liu
authors: National Key Laboratory of Crop Biology, Shandong Agricultural University, Tai-An, Shandong 271018, China.|College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong 271018, China.
authors: Li-Jie
authors: LJ
authors: Zhou
authors: National Key Laboratory of Crop Biology, Shandong Agricultural University, Tai-An, Shandong 271018, China.|College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong 271018, China.
authors: Mou-Jing
authors: MJ
authors: Fang
authors: National Key Laboratory of Crop Biology, Shandong Agricultural University, Tai-An, Shandong 271018, China.|College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong 271018, China.
authors: Qing-Long
authors: QL
authors: Dong
authors: National Key Laboratory of Crop Biology, Shandong Agricultural University, Tai-An, Shandong 271018, China.|College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong 271018, China.
authors: Xiu-Hong
authors: XH
authors: An
authors: National Key Laboratory of Crop Biology, Shandong Agricultural University, Tai-An, Shandong 271018, China.|College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong 271018, China.
authors: Chun-Xiang
authors: CX
authors: You
authors: National Key Laboratory of Crop Biology, Shandong Agricultural University, Tai-An, Shandong 271018, China.|College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong 271018, China.
authors: Yu-Jin
authors: YJ
authors: Hao
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics* / Q000502:physiology*; D005030:Ethylenes / Q000737:chemistry; D035264:Flowers / Q000502:physiology; D005638:Fruit / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics / Q000502:physiology*; D026161:MADS Domain Proteins / Q000235:genetics / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics* / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology; D063146:Polycomb-Group Proteins / Q000235:genetics / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D005030:Ethylenes; D026161:MADS Domain Proteins; C475821:MSI1 protein, Arabidopsis; C108988:MSI1 protein, Lycopersicon esculentum; D009687:Nuclear Proteins; D010940:Plant Proteins; D063146:Polycomb-Group Proteins; D014157:Transcription Factors