title: Ciprofloxacin-imprinted hydrogels for drug sustained release in aqueous media.
abstract: In this study several ciprofloxacin (CFX) imprinted and non-imprinted hydrogels were prepared and evaluated as ocular drug delivery systems in aqueous media. 2-Hydroxyethyl methacrylate (HEMA) was used as a solvent and backbone monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker, methacrylic acid (MAA) as a functional monomer and CFX as the template molecule. CFX-imprinted hydrogels (MIPs) were prepared applying different CFX:MAA molar ratios (1:16, 1:20 and 1:32) in feed composition of monomer solutions. Thermal polymerization was applied and hydrogels were synthesized in a polypropylene mold (0.4 mm thickness). Swelling and binding properties of hydrogels were evaluated in water. Release profile of the MIPs was evaluated in NaCl (0.9%) and artificial tears. The data showed that enhancing the MAA concentration, as a co-monomer, and using molecular imprinting improved binding properties of the synthesized hydrogels. The optimized MIPs with 400 mM MAA and CFX: MAA molar ratio of 1:20 and 1:16 showed the greatest affinity for CFX and the highest ability to control drug release. In vitro antibacterial activity of hydrogels was studied and demonstrated the effect of CFX-loaded hydrogels against Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) isolated from patients' eyes. This study indicated antibacterial efficacy of CFX-loaded MIP hydrogels.
authors: a Students Research Committee, Department of Food and Drug Control, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.
authors: Sajedeh
authors: S
authors: Kioomars
authors: a Students Research Committee, Department of Food and Drug Control, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.
authors: Somayeh
authors: S
authors: Heidari
authors: b Nanotechnology Research Center, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.
authors: Bizhan
authors: B
authors: Malaekeh-Nikouei
authors: a Students Research Committee, Department of Food and Drug Control, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.|c Targeted Drug Delivery Research Center , Mashhad University of Medical Sciences , Mashhad , Iran.
authors: Maryam
authors: M
authors: Shayani Rad
authors: d Department of Pharmaceutical Control, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.
authors: Bahman
authors: B
authors: Khameneh
authors: c Targeted Drug Delivery Research Center , Mashhad University of Medical Sciences , Mashhad , Iran.
authors: Seyed Ahmad
authors: SA
authors: Mohajeri
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000737:chemistry*; D065546:Drug Liberation; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D058105:Polymerization; D011552:Pseudomonas Infections / Q000188:drug therapy; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D020100:Hydrogels; D008689:Methacrylates; C008384:methacrylic acid; D002939:Ciprofloxacin; C005044:hydroxyethyl methacrylate; C004919:ethylene dimethacrylate
keywords: Ciprofloxacin; hydrogels; molecular imprinting; ocular drug delivery systems