title: In Vivo Dynamic Metabolic Changes After Transplantation of Induced Pluripotent Stem Cells for Ischemic Injury.
abstract: This study aimed to investigate in vivo dynamic metabolic changes after transplantation of induced pluripotent stem cells (iPSCs) and iPSC-derived enriched cardiomyocytes (iPSC-CMs) in a rat model of ischemic injury.
  
  
  METHODS
  Serial18F-FDG PET, echocardiographic, immunohistochemical, and immunofluorescence studies were performed after transplantation of iPSCs and iPSC-CMs and compared with embryonic stem cells (ESCs), ESC-CMs, and a phosphate-buffered saline control group of rats with myocardial infarction.
  
  
  RESULTS
  Increased glucose metabolism in periinfarct areas and improved myocardial function were observed in the stem cell transplantation groups compared with the control group, and serial immunofluorescence and immunohistochemical results exhibited the survival and migration of stem cells during the study period.
  
  
  CONCLUSIONS
  Serial18F-FDG PET and echocardiographic imaging studies demonstrated the dynamic metabolic changes and recovery of myocardial function after stem cell transplantation.18F-FDG PET could be a potential approach to evaluating spatiotemporal dynamic metabolic changes in vivo after transplantation of iPSCs or iPSC-CMs for ischemic injury.
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Shuang
authors: S
authors: Wu
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Yuankai
authors: Y
authors: Zhu
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Hao
authors: H
authors: Liu
authors: First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.|Institute of Translational Medicine, Zhejiang University, Hangzhou, China.
authors: Ling
authors: L
authors: Tang
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Ruili
authors: R
authors: Du
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Yehua
authors: Y
authors: Shen
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Jin
authors: J
authors: Feng
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Kai
authors: K
authors: Zhang
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Caiyun
authors: C
authors: Xu
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Shouhong
authors: S
authors: Zhang
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Yao
authors: Y
authors: Chen
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Fahuan
authors: F
authors: Song
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Yunqi
authors: Y
authors: Zhu
authors: Department of Pathology, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, China; and.
authors: Weizhong
authors: W
authors: Gu
authors: First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.|Institute of Translational Medicine, Zhejiang University, Hangzhou, China.
authors: Ping
authors: P
authors: Liang
authors: Nuclear Medicine Department, Hospital Sant Pau, Autonomous University of Barcelona, Barcelona, Spain.
authors: Ignasi
authors: I
authors: Carrió
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Hong
authors: H
authors: Zhang
authors: Department of Nuclear Medicine and Medical PET Center, Second Hospital of Zhejiang University School of Medicine, Hangzhou, China meitian@zju.edu.cn.|Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China.|Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China.
authors: Mei
authors: M
authors: Tian
mesh_terms: D000818:Animals; D004562:Electrocardiography; D019788:Fluorodeoxyglucose F18; D057026:Induced Pluripotent Stem Cells / Q000637:transplantation*; D008297:Male; D017202:Myocardial Ischemia / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000628:therapy; D049268:Positron-Emission Tomography; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019788:Fluorodeoxyglucose F18
keywords: induced PSC-derived cardiomyocytes (iPSC-CMs); induced pluripotent stem cell (iPSC); metabolism; myocardial ischemia; positron emission tomography (PET)