title: Herpetol ameliorates allergic contact dermatitis through regulating T-lymphocytes.
abstract: An immunosuppressant with fewer adverse effects is still urgently needed for increasing numbers of patients suffering from allergic contact dermatitis. In the present study, we aimed to investigate the immunosuppressive activity of herpetol on T-lymphocytes in vitro and in vivo and explore its potential pharmacological mechanism. The results showed that herpetol could effectively inhibit the proliferation of activated T cells and reduce the production of pro-inflammatory cytokines at 5-20μM. Additionally, the ear swelling and inflammatory responses induced by picryl chloride were significantly ameliorated by herpetol at 20-40mg/kg. Moreover, herpetol could cause cell cycle arrest of activated T cells in a dose-dependent manner. Furthermore, herpetol reduced the expression and activity of HIF-1α, Glut1 and LDHA, leading to glycolysis inhibition in activated T cells. Taken together, herpetol showed an immunosuppressive activity against T-cell mediated immune responses in vitro and in vivo, and it has potential for the treatment of immune-related skin diseases.
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors: Xiang
authors: X
authors: Li
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China; Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, China.
authors: Xingqi
authors: X
authors: Wang
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China; School of Life Science and Engineering, and College of Medicine, Southwest Jiaotong University, Chengdu, China.
authors: Hezhong
authors: H
authors: Jiang
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors: Guohui
authors: G
authors: Zhang
authors: School of Life Science and Engineering, and College of Medicine, Southwest Jiaotong University, Chengdu, China.
authors: Rui
authors: R
authors: Tan
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors: Yang
authors: Y
authors: Sun
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors: Xuefeng
authors: X
authors: Wu
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China. Electronic address: rxtan@nju.edu.cn.
authors: Renxiang
authors: R
authors: Tan
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China. Electronic address: molpharm@163.com.
authors: Qiang
authors: Q
authors: Xu
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000494:pharmacology* / Q000627:therapeutic use*; D002453:Cell Cycle / Q000187:drug effects; D017449:Dermatitis, Allergic Contact / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D004423:Ear / Q000473:pathology; D005260:Female; D051272:Glucose Transporter Type 1 / Q000235:genetics; D006019:Glycolysis / Q000187:drug effects; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics; D007166:Immunosuppressive Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D008198:Lymph Nodes / Q000166:cytology; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010853:Picryl Chloride; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
chemical_list: D001572:Benzofurans; D051272:Glucose Transporter Type 1; C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007166:Immunosuppressive Agents; C495678:Slc2a1 protein, mouse; C000617972:herpetol; D010853:Picryl Chloride
keywords: Allergic contact dermatitis; Drug candidate; Glycolysis; Herpetol; T-lymphocytes