title: Combinatory annotation of cell membrane receptors and signalling pathways of Bombyx mori prothoracic glands.
abstract: The cells of prothoracic glands (PG) are the main site of synthesis and secretion of ecdysteroids, the biochemical products of cholesterol conversion to steroids that shape the morphogenic development of insects. Despite the availability of genome sequences from several insect species and the extensive knowledge of certain signalling pathways that underpin ecdysteroidogenesis, the spectrum of signalling molecules and ecdysteroidogenic cascades is still not fully comprehensive. To fill this gap and obtain the complete list of cell membrane receptors expressed in PG cells, we used combinatory bioinformatic, proteomic and transcriptomic analysis and quantitative PCR to annotate and determine the expression profiles of genes identified as putative cell membrane receptors of the model insect species, Bombyx mori, and subsequently enrich the repertoire of signalling pathways that are present in its PG cells. The genome annotation dataset we report here highlights modules and pathways that may be directly involved in ecdysteroidogenesis and aims to disseminate data and assist other researchers in the discovery of the role of such receptors and their ligands.
authors: HybridStat Predictive Analytics, Aiolou 19, Athens 10551, Greece.|Biomedical Sciences Research Center 'Alexander Fleming', Fleming 34, Vari 16672, Greece.
authors: Panagiotis
authors: P
authors: Moulos
authors: Biomedical Sciences Research Center 'Alexander Fleming', Fleming 34, Vari 16672, Greece.
authors: Martina
authors: M
authors: Samiotaki
authors: Biomedical Sciences Research Center 'Alexander Fleming', Fleming 34, Vari 16672, Greece.
authors: George
authors: G
authors: Panayotou
authors: Department of Biology, National and Kapodistrian University of Athens, Athens 15784, Greece.
authors: Skarlatos G
authors: SG
authors: Dedos
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002462:Cell Membrane / Q000378:metabolism; D007301:Insect Hormones / Q000378:metabolism*; D007814:Larva; D040901:Proteomics
chemical_list: D007301:Insect Hormones