title: Associations Between Newly Discovered Polymorphisms of the CEBPD GENE LOCUS and Body Parameters in Sheep.
abstract: An understanding of what effects particular genes can have on body parameters in productive animals is particularly significant for the process of marker-assisted selection. The gene of transcriptional factor CCAAT/enhancer-binding protein delta (CEBPD gene) is involved in the process of growth in animals and is known to be a promising candidate for use as a genomic marker. The structure of the CEBPD gene locus was determined using NimbleGen sequencing technology (Roche, USA). The effect of polymorphisms, which were identified using the aforementioned technology, was investigated in 30 rams of the Manych Merino sheep breed. Twenty-two single nucleotide polymorphisms (SNP) were detected in the CEBPD gene locus. Significantly, two SNPs, namely, g.315T>G and g.327C>T, have been identified for the first time. It was demonstrated that the complex of linked SNPs, consisting of g.301A>T, g.426T>C, and g.1226T>C, had a negligible effect on body parameters in Manych Merino sheep. Animals with the heterozygous type of SNP g.1142C>T exhibited changes solely in the chest and croup width. The newly discovered SNP g.327C>T was proven to have a negative effect on live weight and body size (p < 0.05) in Manych Merino sheep. Sheep with the heterozygous type of g.562G>A and g.3112C>G SNP complex showed an increase in live weight and dimensions (p < 0.05) compared with those of wild homozygous type. Consequently, SNPs g.327C>T, g.562G>A, and g.3112C>G in the CEBPD gene locus can be successfully used as markers in sheep breeding. Future research will evaluate the influence of the aforementioned SNPs on slaughter indicators for sheep meat production.
authors: a Faculty of Veterinary Medicine , Stavropol State Agrarian University , Stavropol , Russian Federation.
authors: Vladimir
authors: V
authors: Trukhachev
authors: a Faculty of Veterinary Medicine , Stavropol State Agrarian University , Stavropol , Russian Federation.
authors: Valentin
authors: V
authors: Skripkin
authors: a Faculty of Veterinary Medicine , Stavropol State Agrarian University , Stavropol , Russian Federation.
authors: Andrey
authors: A
authors: Kvochko
authors: b Stavropol Research Anti-plague Institute , Stavropol , Russian Federation.
authors: Alexander
authors: A
authors: Kulichenko
authors: b Stavropol Research Anti-plague Institute , Stavropol , Russian Federation.
authors: Dmitry
authors: D
authors: Kovalev
authors: b Stavropol Research Anti-plague Institute , Stavropol , Russian Federation.
authors: Sergey
authors: S
authors: Pisarenko
authors: b Stavropol Research Anti-plague Institute , Stavropol , Russian Federation.
authors: Anna
authors: A
authors: Volynkina
authors: c All-Russian Research Institute Of Sheep and Goat Breeding , Stavropol , Russian Federation.
authors: Marina
authors: M
authors: Selionova
authors: c All-Russian Research Institute Of Sheep and Goat Breeding , Stavropol , Russian Federation.
authors: Magomet
authors: M
authors: Aybazov
authors: a Faculty of Veterinary Medicine , Stavropol State Agrarian University , Stavropol , Russian Federation.
authors: Natalia
authors: N
authors: Golovanova
authors: a Faculty of Veterinary Medicine , Stavropol State Agrarian University , Stavropol , Russian Federation.
authors: Olesya
authors: O
authors: Yatsyk
authors: a Faculty of Veterinary Medicine , Stavropol State Agrarian University , Stavropol , Russian Federation.
authors: Alexander
authors: A
authors: Krivoruchko
mesh_terms: D000818:Animals; D049628:Body Size / Q000235:genetics; D001835:Body Weight / Q000235:genetics; D051743:CCAAT-Enhancer-Binding Protein-delta / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D008297:Male; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012756:Sheep / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D051743:CCAAT-Enhancer-Binding Protein-delta
keywords: CCAAT/enhancer-binding protein delta; Manych Merino; marker-assisted selection; sheep; single nucleotide polymorphism