title: Bovine adenovirus type 3 virions cannot be rescuedin vivoafter full-length viral genome transfection in the absence of detectable polypeptide IX.
abstract: Bovine adenovirus type 3 (BAdV3) is being used in the development of potential vehicles for gene therapy and vectored vaccine. To that end, a more comprehensive description of BAdV3 biology is essential. In this study, we focused on the role of pIX in BAdV3 virion rescue after full-length BAdV3 genome transfection. Initially, pIX deletion or initiation codon mutation abolished the production of progeny virions, which suggested that pIX was essential for the rescue of BAdV3 containing a full-length genome. Moreover, through transfection of a panel of pIX mutant BAdV3 genomes, we observed that the conserved N-terminus and the putative leucine zipper element (PLZP) were essential for virion rescue, whereas the C-terminus following the coiled-coil domain was non-essential. In addition, swap of the PLZP element and its following region of BAdV3 pIX to corresponding domains of human adenovirus type 5 (HAdV5) did not affect virion production, whereas swap of the entire pIX abolished production of progeny virions. We suggest that failure of the full-length BAdV3 pIX swap might be due to species specificity of its N-terminus region before the PLZP element.
authors: College of Veterinary Medicine, North-west A&F University, Yangling 712100, China.
authors: Peng
authors: P
authors: Zhang
authors: Chinese Institute of Veterinary Drug Controls, Beijing 100000, China.
authors: Qinghong
authors: Q
authors: Xue
authors: College of Veterinary Medicine, North-west A&F University, Yangling 712100, China.
authors: Jing
authors: J
authors: Ma
authors: College of Veterinary Medicine, North-west A&F University, Yangling 712100, China.
authors: Jingjing
authors: J
authors: Ren
authors: College of Veterinary Medicine, North-west A&F University, Yangling 712100, China.
authors: Shuili
authors: S
authors: Xia
authors: College of Veterinary Medicine, North-west A&F University, Yangling 712100, China.
authors: Lu
authors: L
authors: Zhang
authors: College of Veterinary Medicine, North-west A&F University, Yangling 712100, China.
authors: Wenbin
authors: W
authors: Wang
authors: VIDO-InteVac, University of Saskatchewan Saskatoon, Saskatchewan, Canada.|Vaccinology & Immunotherapeutics program, School of Public Health, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors: Suresh K
authors: SK
authors: Tikoo
authors: College of Veterinary Medicine, North-west A&F University, Yangling 712100, China.
authors: Enqi
authors: E
authors: Du
mesh_terms: D000818:Animals; D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics / Q000502:physiology*; D002417:Cattle / Q000821:virology; D016679:Genome, Viral / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D016350:Leucine Zippers / Q000235:genetics; D017921:Mastadenovirus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D014162:Transfection / Q000662:veterinary; D014771:Virion / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus
keywords: adenovirus; bovine adenovirus type 3; polypeptide IX; viral rescue