title: Mossambicus tilapia (Oreochromis mossambicus) collected from water bodies impacted by urban waste carries extended-spectrum beta-lactamases and integron-bearing gut bacteria.
abstract: Oreochromis mossambicus (Peters 1852) (Tilapia) is one of the most consumed fish globally. Tilapia thrives well in environments polluted by urban waste, which invariably contain antibiotic-resistant bacteria and antibiotic resistance genes (ARGs). Thus, Tilapia surviving in such polluted environments may serve as a potential source for dissemination of ARGs. To investigate this, we isolated bacterial strains from gut of Tilapia found in polluted rivers and lakes near Pune, India, and studied the prevalence of resistance genes by molecular methods. A total of 91 bacterial strains were obtained, which include fish pathogens and human pathogens such as Aeromonas hydrophila, Klebsiella pneumoniae, E. coli, Serratia marcescens, Enterobacter spp. and Shigella spp. Overall the prevalence of class 1 integrons, class 2 integrons, extended-spectrum betalactamases (ESBLs) blaCTX-M, blaSHV and aac(6')-Ib-cr gene was 38 percent, 24 percent, 38 percent, 31 percent and 31 percent respectively. Forty-two percent of the Enterobacteriaceae strains carried blaCTX-M gene, which is a common ESBL gene in clinics. The study demonstrates that tilapia found in the polluted waters can serve as reservoirs and an alternative route for human exposure to clinically important ARG-carrying bacteria. The consumption and handling of these fish may pose a potential health risk.
authors: Microbial Culture Collection, National Centre for Cell Science, Pune 411 007, India.
authors: Nachiket P
authors: NP
authors: Marathe
authors: Swapnil S
authors: SS
authors: Gaikwad
authors: Ankita A
authors: AA
authors: Vaishampayan
authors: Mandar H
authors: MH
authors: Rasane
authors: Yogesh S
authors: YS
authors: Shouche
authors: Wasudev N
authors: WN
authors: Gade
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004755:Enterobacteriaceae / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D004787:Environmental Pollution; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D000069196:Gastrointestinal Microbiome / Q000235:genetics; D006801:Humans; D007194:India; D007220:Industrial Waste / Q000009:adverse effects; D060106:Lakes / Q000382:microbiology; D045483:Rivers / Q000382:microbiology; D017210:Tilapia / Q000235:genetics / Q000382:microbiology*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D007220:Industrial Waste; D001618:beta-Lactamases