title: Isolation and Characterization of an Escherichia albertii Strain Producing Three Different Toxins from a Child with Diarrhea.
abstract: Here, we report a bacterium-isolated as the sole pathogen from a child with diarrhea-harboring eae and 2 different cytolethal distending toxin genes (cdt) that are homologous to Escherichia coli cdt-I and cdt-II. The bacterium was originally identified as atypical E. coli by conventional biochemical testing, but was finally identified as E. albertii by multilocus sequence analysis, which is the only method that can currently differentiate E. albertii from E. coli. The Shiga toxin 2f (stx2f) genes were also detected in the strain. Production of these 3 toxins was confirmed by western blotting and/or a cytotoxicity assay using eukaryotic cell lines. This is the first report showing the biological activity of CDT-I, CDT-II, and Stx2f in E. albertii.
authors: Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
authors: Atsushi
authors: A
authors: Hinenoya
authors: Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
authors: Noritomo
authors: N
authors: Yasuda
authors: Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
authors: Takumi
authors: T
authors: Hibino
authors: Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
authors: Ayaka
authors: A
authors: Shima
authors: Department of Pediatrics, Mizushima General Hospital.
authors: Akira
authors: A
authors: Nagita
authors: Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
authors: Teizo
authors: T
authors: Tsukamoto
authors: Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
authors: Shinji
authors: S
authors: Yamasaki
mesh_terms: D001427:Bacterial Toxins / Q000032:analysis* / Q000145:classification / Q000235:genetics; D015153:Blotting, Western; D003967:Diarrhea / Q000382:microbiology* / Q000473:pathology*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000473:pathology*; D006801:Humans; D007223:Infant; D058885:Multilocus Sequence Typing; D037521:Virulence Factors / Q000032:analysis* / Q000145:classification / Q000235:genetics
chemical_list: D001427:Bacterial Toxins; D037521:Virulence Factors
keywords: CDT-I; CDT-II; Escherichia albertii; Stx2f; cytolethal distending toxin