title: Genome-based insights into the resistome and mobilome of multidrug-resistant Aeromonas sp. ARM81 isolated from wastewater.
abstract: The draft genome of multidrug-resistant Aeromonas sp. ARM81 isolated from a wastewater treatment plant in Warsaw (Poland) was obtained. Sequence analysis revealed multiple genes conferring resistance to aminoglycosides, β-lactams or tetracycline. Three different β-lactamase genes were identified, including an extended-spectrum β-lactamase gene blaPER-1. The antibiotic susceptibility was experimentally tested. Genome sequencing also allowed us to investigate the plasmidome and transposable mobilome of ARM81. Four plasmids, of which two carry phenotypic modules (i.e., genes encoding a zinc transporter ZitB and a putative glucosyltransferase), and 28 putative transposase genes were identified. The mobility of three insertion sequences (isoforms of previously identified elements ISAs12, ISKpn9 and ISAs26) was confirmed using trap plasmids.
authors: Faculty of Biology, Institute of Microbiology, Department of Bacterial Genetics, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
authors: Marcin
authors: M
authors: Adamczuk
authors: Faculty of Biology, Institute of Microbiology, Department of Bacterial Genetics, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland. ldziewit@biol.uw.edu.pl.
authors: Lukasz
authors: http://orcid.org/0000-0002-5057-2811
authors: L
authors: Dziewit
mesh_terms: D000333:Aeromonas / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004251:DNA Transposable Elements; D024881:Drug Resistance, Bacterial; D016680:Genome, Bacterial; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011044:Poland; D017422:Sequence Analysis, DNA; D062065:Wastewater / Q000382:microbiology*; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D062065:Wastewater; D001618:beta-Lactamases
keywords: Aeromonas sp. ARM81; Antibiotic resistance gene; Genome; Plasmid; Transposable element