title: High Mobility Group Box1 Protein Is Involved in Endoplasmic Reticulum Stress Induced by Clostridium difficile Toxin A.
abstract: High Mobility Group Box1 (HMGB1), a damage-associated inflammatory factor, plays an important role in the pathogenesis of numerous chronic inflammatory and autoimmune diseases. In this study, the role of the HMGB1 in TcdA-induced ER stress was identified. Clostridium difficile toxin A is one of the major virulence factors of C. difficile infection (CDI) and has been proved to induce apoptotic cell death through ER stress. Our results showed that HMGB1 might play an important role in the TcdA-induced ER stress and unfolded protein response. HMGB1 activated molecular markers and induced the C/EBP homologous protein upregulation (CHOP). This study may provide the essential information for better understanding of the molecular mechanisms involved in CDI.
authors: School of Bioscience & Bioengineering, South China University of Technology, Guangzhou 510006, China.
authors: Ji
authors: J
authors: Liu
authors: School of Bioscience & Bioengineering, South China University of Technology, Guangzhou 510006, China.
authors: Yi
authors: Y
authors: Ma
authors: School of Bioscience & Bioengineering, South China University of Technology, Guangzhou 510006, China.
authors: Chun-Li
authors: CL
authors: Sun
authors: School of Bioscience & Bioengineering, South China University of Technology, Guangzhou 510006, China; Guangdong Province Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou 510006, China.
authors: Shan
authors: 0000-0002-5211-0872
authors: S
authors: Li
authors: School of Bioscience & Bioengineering, South China University of Technology, Guangzhou 510006, China.
authors: Ju-Fang
authors: 0000-0002-9908-1001
authors: JF
authors: Wang
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism*; D004768:Enterotoxins / Q000008:administration & dosage*; D024243:HMGB1 Protein / Q000378:metabolism*; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D024243:HMGB1 Protein; C582808:HMGB1 protein, mouse; D017382:Reactive Oxygen Species; C041348:tcdA protein, Clostridium difficile