title: Sirtuin 5 is Anti-apoptotic and Anti-oxidative in Cultured SH-EP Neuroblastoma Cells.
abstract: As a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase, demalonylase, and desuccinylase, sirtuin 5 (SIRT5) in host cells has been reportedly observed in the mitochondria, in the cytosol/cytoplasm or in the nucleus. Various functional roles of SIRT5 have also been described in cellular metabolism, energy production, detoxification, oxidative stress, and apoptosis, but some of the reported results are seemingly inconsistent or even contradictory to one another. Using immunocytochemistry, molecular biology, gene transfection, and flow cytometry, we investigated the expression, subcellular distribution, and possible functional roles of SIRT5 in regulating apoptosis and oxidative stress of cultured SH-EP neuroblastoma cells. Both endogenous and transfected exogenous SIRT5 were observed in mitochondria of host SH-EP cells. Overexpression of SIRT5 markedly protected SH-EP cells from apoptosis induced by staurosporine or by incubation in Hank's balanced salt solution. SIRT5 also lowered the level of oxidative stress and countered the toxicity of hydrogen peroxide to SH-EP cells. It was suggested that the anti-apoptotic role of SIRT5 was mediated, at least in part, by its anti-oxidative effect in SH-EP neuroblastoma cells although the involved molecular mechanisms remain to be elucidated in details.
authors: Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore. antlfy@nus.edu.sg.
authors: Fengyi
authors: 0000-0003-1162-6212
authors: F
authors: Liang
authors: Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore.|Department of Pathology, Kunming Medical University, Kunming, 650055, Yunnan, People's Republic of China.
authors: Xie
authors: X
authors: Wang
authors: Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore.
authors: Suet Hui
authors: SH
authors: Ow
authors: Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore.
authors: Wangxue
authors: W
authors: Chen
authors: Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 119074, Singapore.
authors: Wei Chen
authors: WC
authors: Ong
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism; D000066829:Neuroprotection / Q000187:drug effects / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D037761:Sirtuins / Q000235:genetics / Q000378:metabolism*; D019311:Staurosporine / Q000633:toxicity; D014162:Transfection
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; C508544:SIRT5 protein, human; D037761:Sirtuins; D019311:Staurosporine
keywords: Apoptosis; Mitochondria; Reactive oxygen species (ROS); SH-EP neuroblastoma cell line; Sirtuin 5 (sirt5); Staurosporine