title: Paenibacillusoryzae sp. nov., isolated from rice roots.
abstract: A novel endophytic bacterium, strain 1DrF-4T, isolated from rice roots, was characterized on the basis of its phenotypic characteristics and genotypic information. The novel strain was Gram-positive-staining, endospore-forming, facultatively anaerobic, motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1DrF-4T formed a monophyletic clade within the genus Paenibacillus. The most phylogenetically related species was Paenibacillus pinesoli KACC 17472T, with which strain 1DrF-4T showed 16S rRNA gene sequence similarity of 95.2 %. 16S rRNA gene sequence similarities with type strains of other species of the genus Paenibacillus were less than 95 %. The predominant cellular fatty acids were anteiso-C15 : 0 (61.1 %) and C16 : 0 (11.1 %), which is one of the characteristic traits of the genus Paenibacillus. The quinone system contained exclusively menaquinone MK-7. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid and an unknown phospholipid. The DNA G+C content was 50.16 mol%, which was within the range reported for species of the genus Paenibacillus. Characterization by genotypic, chemotaxonomic and phenotypic analysis indicated that strain 1DrF-4T (=ACCC 19927T=JCM 30486T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillusoryzae sp. nov. is proposed.
authors: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.|College of Life Science, The Yangtze University, Jingzhou 434025, PR China.
authors: Jun
authors: J
authors: Zhang
authors: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors: Xiao-Tong
authors: XT
authors: Ma
authors: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.|Qiyang Agro-ecosystem of National Field Experimental Station, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Qiyang 426182, PR China.
authors: Jun-Sheng
authors: JS
authors: Gao
authors: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors: Juan-Juan
authors: JJ
authors: Zhao
authors: School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, PR China.
authors: Hua-Qun
authors: HQ
authors: Yin
authors: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors: Cai-Wen
authors: CW
authors: Zhang
authors: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors: Rui-Jie
authors: RJ
authors: Zhang
authors: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors: Xiao-Xia
authors: XX
authors: Zhang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000382:microbiology*; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7