title: Superabsorbent 3D Scaffold Based on Electrospun Nanofibers for Cartilage Tissue Engineering.
abstract: Electrospun nanofibers have been used for various biomedical applications. However, electrospinning commonly produces two-dimensional (2D) membranes, which limits the application of nanofibers for the 3D tissue engineering scaffold. In the present study, a porous 3D scaffold (3DS-1) based on electrospun gelatin/PLA nanofibers has been prepared for cartilage tissue regeneration. To further improve the repairing effect of cartilage, a modified scaffold (3DS-2) cross-linked with hyaluronic acid (HA) was also successfully fabricated. The nanofibrous structure, water absorption, and compressive mechanical properties of 3D scaffold were studied. Chondrocytes were cultured on 3D scaffold, and their viability and morphology were examined. 3D scaffolds were also subjected to an in vivo cartilage regeneration study on rabbits using an articular cartilage injury model. The results indicated that 3DS-1 and 3DS-2 exhibited superabsorbent property and excellent cytocompatibility. Both these scaffolds present elastic property in the wet state. An in vivo study showed that 3DS-2 could enhance the repair of cartilage. The present 3D nanofibrous scaffold (3DS-2) would be promising for cartilage tissue engineering application.
authors: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University , Shanghai 201620, China.
authors: Weiming
authors: W
authors: Chen
authors: Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital , 600 Yishan Road, Shanghai 200233, China.
authors: Shuai
authors: S
authors: Chen
authors: Faculty of Engineering and Industrial Sciences, Swinburne University of Technology , Hawthorn, Vic 3122, Australia.
authors: Yosry
authors: Y
authors: Morsi
authors: Department of Chemistry, College of Science, King Saud University , Riyadh 11451, Kingdom of Saudi Arabia.|Department of Chemistry, Faculty of Science, Tanta University , Tanta 31527, Egypt.
authors: Hany
authors: H
authors: El-Hamshary
authors: Department of Chemistry, College of Science, King Saud University , Riyadh 11451, Kingdom of Saudi Arabia.|Department of Chemistry, Faculty of Science, Tanta University , Tanta 31527, Egypt.
authors: Mohamed
authors: M
authors: El-Newhy
authors: Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital , 600 Yishan Road, Shanghai 200233, China.
authors: Cunyi
authors: C
authors: Fan
authors: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University , Shanghai 201620, China.
authors: Xiumei
authors: X
authors: Mo
mesh_terms: D000818:Animals; D019902:Chondrocytes; D005780:Gelatin; D057139:Nanofibers; D011091:Polyesters; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; D005780:Gelatin
keywords: 3D scaffold; cartilage tissue engineering; cross-linking; electrospun nanofiber; superabsorbent