title: Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with recessive dystrophic epidermolysis bullosa (RDEB) carrying two compound heterozygous mutations in the COL7A1 gene.
abstract: Expanded human T cells from a Japanese female with recessive dystrophic epidermolysis bullosa (RDBE) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The authenticity of established iPSC line, RDEB-iPSC26, was confirmed by the expressions of stem cell markers and the differentiation capability into three germ layer. RDEB-iPSC26 may be a useful cell resource for the establishment of in vitro RDEB modeling and the study for developing gene and cell therapy.
authors: Department of Dermatology, The Jikei University School of Medicine, Japan. Electronic address: seafowl@jikei.ac.jp.
authors: Munenari
authors: M
authors: Itoh
authors: Department of Dermatology, The Jikei University School of Medicine, Japan.
authors: Shiho
authors: S
authors: Kawagoe
authors: Department of Stem Cell Therapy Science, Graduate School of Medicine, Osaka University, Japan.
authors: Katsuto
authors: K
authors: Tamai
authors: Division of Regenerative Medicine, The Jikei University School of Medicine, Japan.
authors: Hirotaka James
authors: HJ
authors: Okano
authors: Department of Dermatology, The Jikei University School of Medicine, Japan.
authors: Hidemi
authors: H
authors: Nakagawa