title: Preparation and Applications of Organotypic Thymic Slice Cultures.
abstract: Thymic selection proceeds in a unique and highly organized thymic microenvironment resulting in the generation of a functional, self-tolerant T cell repertoire. In vitro models to study T lineage commitment and development have provided valuable insights into this process. However, these systems lack the complete three-dimensional thymic milieu necessary for T cell development and, therefore, are incomplete approximations of in vivo thymic selection. Some of the challenges related to modeling T cell development can be overcome by using in situ models that provide an intact thymic microenvironment that fully supports thymic selection of developing T cells. Thymic slice organotypic cultures complement existing in situ techniques. Thymic slices preserve the integrity of the thymic cortical and medullary regions and provide a platform to study development of overlaid thymocytes of a defined developmental stage or of endogenous T cells within a mature thymic microenvironment. Given the ability to generate ~20 slices per mouse, thymic slices present a unique advantage in terms of scalability for high throughput experiments. Further, the relative ease in generating thymic slices and potential to overlay different thymic subsets or other cell populations from diverse genetic backgrounds enhances the versatility of this method. Here we describe a protocol for the preparation of thymic slices, isolation and overlay of thymocytes, and dissociation of thymic slices for flow cytometric analysis. This system can also be adapted to study non-conventional T cell development as well as visualize thymocyte migration, thymocyte-stromal cell interactions, and TCR signals associated with thymic selection by two-photon microscopy.
authors: Centre de Recherche, Hôpital Maisonneuve-Rosemont; Department of Microbiology, Infectiology and Immunology, Université de Montréal.
authors: Aditi
authors: A
authors: Sood
authors: Centre de Recherche, Hôpital Maisonneuve-Rosemont; Department of Microbiology, Infectiology and Immunology, Université de Montréal.
authors: Mengqi
authors: M
authors: Dong
authors: Centre de Recherche, Hôpital Maisonneuve-Rosemont; Department of Medicine, Université de Montréal; heather.melichar@umontreal.ca.
authors: Heather J
authors: HJ
authors: Melichar
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D005434:Flow Cytometry; D051379:Mice; D023421:Models, Animal; D017154:Stromal Cells / Q000166:cytology*; D013601:T-Lymphocytes / Q000166:cytology; D060168:Thymocytes / Q000166:cytology*; D013950:Thymus Gland / Q000166:cytology*