title: An integrated lab-on-a-chip-based electrochemical biosensor for rapid and sensitive detection of cancer biomarkers.
abstract: Recent advances in the area of biosensor technology and microfluidic applications have enabled the miniaturisation of the sensing platforms. Here we describe a new integrated and fully automated lab-on-a-chip-based biosensor device prototype (MiSens) that has potential to be used for point-of-care cancer biomarker testing. The key features of the device include a new biochip, a device integrated microfluidic system and real-time amperometric measurements during the flow of enzyme substrate. For ease of use, a new plug and play type sensor chip docking station has been designed. This system allows the formation of an ∼7 μL capacity flow cell on the electrode array with the necessary microfluidic and electronic connections with one move of a handle. As a case study, the developed prototype has been utilised for the detection of prostate-specific antigen (PSA) level in serum that is routinely used as a biomarker for the diagnosis of prostate cancer. The patient samples from a nearby hospital have been collected and tested using the MiSens device, and the results have been compared to the hospital results. The obtained results indicate the potential of the MiSens device as a useful tool for point-of-care testing. Graphical abstract Microfluidics integrated and automated electrochemical biosensor device "MiSens" has been designed and fabricated by a multidisciplinary team and utilised to detect PSA from clinical samples.
authors: Bioelectronic Devices and Systems Group, UEKAE-BILGEM-The Scientific and Technological Research Council of Turkey (TUBITAK), 41470, Gebze, Kocaeli, Turkey. yildiz.uludag@tubitak.gov.tr.
authors: Yildiz
authors: Y
authors: Uludag
authors: Kartal Dr .Lütfi Kırdar Education and Research Hospital Urology Clinic, 34890, Kartal, İstanbul, Turkey.
authors: Fehmi
authors: F
authors: Narter
authors: Kartal Dr .Lütfi Kırdar Education and Research Hospital Urology Clinic, 34890, Kartal, İstanbul, Turkey.
authors: Erkin
authors: E
authors: Sağlam
authors: Bioelectronic Devices and Systems Group, UEKAE-BILGEM-The Scientific and Technological Research Council of Turkey (TUBITAK), 41470, Gebze, Kocaeli, Turkey.
authors: Güzin
authors: G
authors: Köktürk
authors: Bioelectronic Devices and Systems Group, UEKAE-BILGEM-The Scientific and Technological Research Council of Turkey (TUBITAK), 41470, Gebze, Kocaeli, Turkey.
authors: M Yağmur
authors: MY
authors: Gök
authors: Bioelectronic Devices and Systems Group, UEKAE-BILGEM-The Scientific and Technological Research Council of Turkey (TUBITAK), 41470, Gebze, Kocaeli, Turkey.
authors: Mete
authors: M
authors: Akgün
authors: Bioelectronic Devices and Systems Group, UEKAE-BILGEM-The Scientific and Technological Research Council of Turkey (TUBITAK), 41470, Gebze, Kocaeli, Turkey.
authors: Serkan
authors: S
authors: Barut
authors: Bioelectronic Devices and Systems Group, UEKAE-BILGEM-The Scientific and Technological Research Council of Turkey (TUBITAK), 41470, Gebze, Kocaeli, Turkey.
authors: Sinan
authors: S
authors: Budak
mesh_terms: D057205:Automation, Laboratory; D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008297:Male; D046210:Microfluidic Analytical Techniques; D019095:Point-of-Care Systems; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D017430:Prostate-Specific Antigen
keywords: Amperometry; Biosensor; Cancer biomarker; Integrated microfluidics; MiSens; Real-time electrochemical profiling