title: High-throughput method for the quantitation of metabolites and co-factors from homocysteine-methionine cycle for nutritional status assessment.
abstract: OBJECTIVE
  There is increasing interest in the profiling and quantitation of methionine pathway metabolites for health management research. Currently, several analytical approaches are required to cover metabolites and co-factors.
  
  
  RESULTS
  We report the development and the validation of a method for the simultaneous detection and quantitation of 13 metabolites in red blood cells. The method, validated in a cohort of healthy human volunteers, shows a high level of accuracy and reproducibility.
  
  
  CONCLUSIONS
  This high-throughput protocol provides a robust coverage of central metabolites and co-factors in one single analysis and in a high-throughput fashion. In large-scale clinical settings, the use of such an approach will significantly advance the field of nutritional research in health and disease.
authors: Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland.
authors: Laeticia
authors: L
authors: Da Silva
authors: Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland.
authors: Sebastiano
authors: S
authors: Collino
authors: Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland.
authors: Ornella
authors: O
authors: Cominetti
authors: Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland.
authors: Francois-Pierre
authors: FP
authors: Martin
authors: Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland.
authors: Ivan
authors: I
authors: Montoliu
authors: Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland.
authors: Sergio Oller
authors: SO
authors: Moreno
authors: Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland.
authors: John
authors: J
authors: Corthesy
authors: Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland.
authors: Jim
authors: J
authors: Kaput
authors: Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland.
authors: Martin
authors: M
authors: Kussmann
authors: Department of Pediatrics, Faculty of Medicine, Nutrition & Metabolism, University of São Paulo, Bandeirantes Avenue, HCFMRP Campus USP, 3900, Ribeirão Preto, SP 14049-900, Brazil.
authors: Jacqueline Pontes
authors: JP
authors: Monteiro
authors: Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Quarter, CH-1015 Lausanne, Switzerland.
authors: Seu Ping
authors: SP
authors: Guiraud
mesh_terms: D000293:Adolescent; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015331:Cohort Studies; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D057166:High-Throughput Screening Assays / Q000379:methods; D006710:Homocysteine / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D057230:Limit of Detection; D008297:Male; D053858:Metabolic Networks and Pathways; D008715:Methionine / Q000032:analysis / Q000378:metabolism*; D009752:Nutritional Status; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006710:Homocysteine; D008715:Methionine
keywords: UPLC–MS/MS; high throughput; methionine pathway; one-carbon metabolism; red blood cells