title: Inhibiting the Mitochondrial Calcium Uniporter during Development Impairs Memory in Adult Drosophila.
abstract: The uptake of cytoplasmic calcium into mitochondria is critical for a variety of physiological processes, including calcium buffering, metabolism, and cell survival. Here, we demonstrate that inhibiting the mitochondrial calcium uniporter in the Drosophila mushroom body neurons (MBn)-a brain region critical for olfactory memory formation-causes memory impairment without altering the capacity to learn. Inhibiting uniporter activity only during pupation impaired adult memory, whereas the same inhibition during adulthood was without effect. The behavioral impairment was associated with structural defects in MBn, including a decrease in synaptic vesicles and an increased length in the axons of the αβ MBn. Our results reveal an in vivo developmental role for the mitochondrial uniporter complex in establishing the necessary structural and functional neuronal substrates for normal memory formation in the adult organism.
authors: Department of Neuroscience, The Scripps Research Institute Florida, Jupiter, FL 33458, USA.
authors: Ilaria
authors: I
authors: Drago
authors: Department of Neuroscience, The Scripps Research Institute Florida, Jupiter, FL 33458, USA. Electronic address: rdavis@scripps.edu.
authors: Ronald L
authors: RL
authors: Davis
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001369:Axons / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D027682:Cation Transport Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000254:growth & development* / Q000502:physiology*; D020868:Gene Silencing; D008568:Memory / Q000502:physiology*; D008928:Mitochondria / Q000378:metabolism; D024521:Mushroom Bodies / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D012903:Smell; D013572:Synaptic Vesicles / Q000378:metabolism
chemical_list: D027682:Cation Transport Proteins; D029721:Drosophila Proteins; C000622740:MCU protein, Drosophila; D002118:Calcium