title: Internalization of Vectored Liposomes in a Culture of Poorly Differentiated Tumor Cells.
abstract: Internalization of liposomal nanocontainers conjugated with monoclonal antibodies to VEGF, VEGFR2 (KDR), and proteins overproduced in the tumor tissue was studied in vitro on cultures of poorly differentiated tumor cells. Comparative analysis of accumulation of vectored liposomes in the tumor cells was performed by evaluating co-localization of labeled containers and cell organelles by laser scanning confocal microscopy. We observed nearly 2 times more active penetration and accumulation of liposomes vectored with antibodies in the tumor cells in comparison with non-vectored liposomes. Selective clathrin-dependent penetration of vectored liposomes into tumor cells was demonstrated by using pharmacological agents inhibiting endocytosis.
authors: Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University, Moscow, Russia. proximopm@gmail.com.
authors: P A
authors: PA
authors: Mel'nikov
authors: Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University, Moscow, Russia.
authors: V P
authors: VP
authors: Baklaushev
authors: Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University, Moscow, Russia.
authors: A N
authors: AN
authors: Gabashvili
authors: Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University, Moscow, Russia.|Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors: N V
authors: NV
authors: Nukolova
authors: Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors: A B
authors: AB
authors: Levinsky
authors: Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University, Moscow, Russia.|Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors: V P
authors: VP
authors: Chehonin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D045744:Cell Line, Tumor; D005910:Glioma / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism*; D018613:Microscopy, Confocal; D051381:Rats; D042461:Vascular Endothelial Growth Factor A / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D008081:Liposomes; D042461:Vascular Endothelial Growth Factor A
keywords: glioma; internalization; liposomes; monoclonal antibodies to VEGF and VEGFR2 (KDR); targeted delivery