title: Characterization of VvPAL-like promoter from grapevine using transgenic tobacco plants.
abstract: A 2000-bp 5'-flanking region of VvPAL-like was isolated from 'Summer Black' grapevine by PCR amplification, named pVvPAL-like. To gain a better understanding of the expression and regulatory mechanism of VvPAL-like, a chimeric expression unit consisting of the β-glucuronidase (GUS) reporter gene under the control of a 2000-bp fragment of the VvPAL-like promoter was transformed into tobacco via Agrobacterium tumefaciens. Histochemical staining showed that the full-length promoter directs efficient expression of the reporter gene in cotyledons and hypocotyls, stigma, style, anthers, pollen, ovary, trichomes, and vascular bundles of transgenic plants. A series of 5' progressive deletions of the promoter revealed the presence of a negative regulatory region (-424 to -292) in the VvPAL-like promoter. Exposure of the transgenic tobacco plants to various abiotic stresses demonstrated that the full-length construct could be induced by light, copper (Cu), abscisic acid (ABA), indole-3-acetic (IAA), methyl jasmonate (MeJA) (N-1-naphthylphthalamic acid), ethylene, and drought. Furthermore, the ethylene-responsive region was found to be located in the -1461/-930 fragment, while the element(s) for the MeJA-responsive expression may be present in the -424/-292 region in the VvPAL-like promoter. These findings will help us to better understand the molecular mechanisms by which VvPAL-like participates in biosynthesis of flavonoids and stress responses.
authors: Key Laboratory of Genetics and Fruit development, College of Horticulture, Nanjing Agricultural University, Nanjing city, Jiangsu Province, People's Republic of China.
authors: SongTao
authors: S
authors: Jiu
authors: Key Laboratory of Genetics and Fruit development, College of Horticulture, Nanjing Agricultural University, Nanjing city, Jiangsu Province, People's Republic of China.
authors: Chen
authors: C
authors: Wang
authors: Key Laboratory of Genetics and Fruit development, College of Horticulture, Nanjing Agricultural University, Nanjing city, Jiangsu Province, People's Republic of China.
authors: Ting
authors: T
authors: Zheng
authors: Key Laboratory of Genetics and Fruit development, College of Horticulture, Nanjing Agricultural University, Nanjing city, Jiangsu Province, People's Republic of China.
authors: Zhongjie
authors: Z
authors: Liu
authors: Key Laboratory of Genetics and Fruit development, College of Horticulture, Nanjing Agricultural University, Nanjing city, Jiangsu Province, People's Republic of China.
authors: XiangPeng
authors: X
authors: Leng
authors: Key Laboratory of Genetics and Fruit development, College of Horticulture, Nanjing Agricultural University, Nanjing city, Jiangsu Province, People's Republic of China.
authors: Tariq
authors: T
authors: Pervaiz
authors: Department of Plant Molecular Biotechnology, Institute of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
authors: Abolfazl
authors: A
authors: Lotfi
authors: Key Laboratory of Genetics and Fruit development, College of Horticulture, Nanjing Agricultural University, Nanjing city, Jiangsu Province, People's Republic of China. fanggg@njau.edu.cn.
authors: JingGui
authors: J
authors: Fang
authors: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing city, Jiangsu Province, People's Republic of China. xmwang525@163.com.
authors: XiaoMin
authors: X
authors: Wang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000085:Acetates / Q000494:pharmacology; D016960:Agrobacterium tumefaciens / Q000235:genetics; D003300:Copper / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D055864:Droughts; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics; D008027:Light; D054883:Oxylipins / Q000494:pharmacology; D010797:Phthalimides / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D014026:Nicotiana / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*; D027843:Vitis / Q000235:genetics*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010797:Phthalimides; C002687:alpha-naphthylphthalamic acid; D000040:Abscisic Acid; D003300:Copper; C072239:methyl jasmonate; C036216:ethylene; D005966:Glucuronidase
keywords: Abiotic stress; Phenylalanine ammonia-lyase-like; Promoter; Spatial and temporal expression; Transgenic tobacco