title: Effects of MBL-associated serine protease-2 (MASP-2) on liquefaction and ulceration in rabbit skin model of tuberculosis.
abstract: Tuberculosis is a chronic infectious disease, which caused by Mycobacterium tuberculosis. It typically affects the functions of the lung and causes high morbidity and mortality rates worldwide. The lectin pathway, one of the complement cascade systems, provides the primary line of defense against invading pathogens. However, what is the specific effection between tuberculosis and complement is unknown. Mannose-binding lectin (MBL), a recognition subunit, binds to arrays of carbohydrates on the surfaces of pathogens, which results in the activation of MBL-associated serine protease-2 to trigger a downstream reaction cascade of complement system. The effects of human MBL-associated serine protease-2 (hMASP-2) were assessed in a rabbit-skin model by intradermal injection of 5 × 106viable BCG bacilli. The rAd-hMASP-2 accelerated the formation of liquefaction and healing of the granuloma lesions, reduced the bacteria loads of the skin nodules. The serum levels of IL-2 and IFN-γ were significantly increasing during the granuloma and liquefaction phases in the rAd-hMASP-2 group. This study suggests that hMASP-2 can induce a protective efficacy in BCG-infected rabbit skin models, which affects both the progress of lesions and the survival of the mycobacteria within them.
authors: Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China; Lanzhou University Second Hospital, Lanzhou, 730030, China.
authors: Xinfang
authors: X
authors: Dong
authors: Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China.
authors: Yanping
authors: Y
authors: Luo
authors: Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China.
authors: Qi
authors: Q
authors: Gao
authors: Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China.
authors: Xiaoling
authors: X
authors: Lu
authors: Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China.
authors: Qian
authors: Q
authors: Wang
authors: Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China.
authors: Yuan
authors: Y
authors: Zhang
authors: Institute of Pathogenic Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China.
authors: Xun
authors: X
authors: Liu
authors: Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China.
authors: Lifeng
authors: L
authors: Zhang
authors: Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China.
authors: Jingqiu
authors: J
authors: Wang
authors: Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China; Gansu Key Lab of Evidence Based Medicine and Clinical Transfer Medicine & Lanzhou Center for Tuberculosis Research, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China. Electronic address: maxm@lzu.edu.cn.
authors: Xingming
authors: X
authors: Ma
authors: Institute of Pathogenic Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China; Gansu Key Lab of Evidence Based Medicine and Clinical Transfer Medicine & Lanzhou Center for Tuberculosis Research, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730030, China. Electronic address: bdzhu@lzu.edu.cn.
authors: Bingdong
authors: B
authors: Zhu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006099:Granuloma / Q000473:pathology; D006801:Humans; D050606:Mannose-Binding Protein-Associated Serine Proteases / Q000378:metabolism*; D009163:Mycobacterium bovis / Q000276:immunology*; D011817:Rabbits; D012867:Skin / Q000276:immunology / Q000473:pathology*; D014382:Tuberculosis, Cutaneous / Q000276:immunology* / Q000382:microbiology / Q000473:pathology*; D014456:Ulcer / Q000276:immunology* / Q000382:microbiology / Q000473:pathology*; D014945:Wound Healing
chemical_list: D050606:Mannose-Binding Protein-Associated Serine Proteases
keywords: Complement; MBL-associated serine protease-2; Rabbit; Tuberculosis