title: MicroRNA-140-5p targets insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1) to suppress cervical cancer growth and metastasis.
abstract: MicroRNAs (miRNAs) are a class of small non-coding RNA molecules that play important roles in carcinogenesis and tumor progression. Previous studies have revealed that MicroRNA-140-5p (miR-140-5p) was abnormally expressed in several cancers. However, its function and possible mechanism in cervical cancer (CC) remains unknown. In this study, the data mining results showed that miR-140-5p was down-regulated in CC specimens and the down-regulation of miR-140-5p was associated with CC poor prognosis. These observations prompted us to further investigate the roles and mechanisms of miR-140-5p in human CC pathogenesis. We found that the over-expression/inhibition of miR-140-5p significantly decreased/increased cell proliferation, migration, and invasion in CC cells in vitro. Meanwhile, the results from in vivo assays showed that the over-expression of miR-140-5p induced significantly suppression of tumor growth and metastasis in nude mice. Furthermore, Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1) was identified as a direct target of miR-140-5p, and both gain-of-function and loss-of-function assays revealed that IGF2BP1 is also a functional target of miR-140-5p. Taken together, our findings suggested a novel miR-140-5p-IGF2BP1 regulatory circuit for CC pathogenesis, and miR-140-5p may be a potential target for CC therapy.
authors: Department of Obstetrics and Gynecology, Changsha Central Hospital, Changsha, China.
authors: Yanlin
authors: Y
authors: Su
authors: Department of Epidemiology and Health Statistcs, XiangYa School of Public Health, Central South University, Changsha, China.
authors: Jie
authors: J
authors: Xiong
authors: Medical Research Center, Changsha Central Hospital, Changsha, China.
authors: Jinyue
authors: J
authors: Hu
authors: Department of Obstetrics and Gynecology, Changsha Central Hospital, Changsha, China.
authors: Xin
authors: X
authors: Wei
authors: Department of Obstetrics and Gynecology, Changsha Central Hospital, Changsha, China.
authors: Xuelian
authors: X
authors: Zhang
authors: Department of Obstetrics and Gynecology, Changsha Central Hospital, Changsha, China.
authors: Lijuan
authors: L
authors: Rao
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009362:Neoplasm Metastasis; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D014183:Transplantation, Heterologous; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; C406144:IGF2BP1 protein, human; D035683:MicroRNAs; C544302:Mirn140 microRNA, human; D016601:RNA-Binding Proteins
keywords: IGF2BP1; cervical cancer; microRNA-140-5p; tumor suppressor