title: Inflammation has a role in urethane‑induced lung cancer in C57BL/6J mice.
abstract: Lung cancer is a common and highly frequent cause of cancer‑associated mortality worldwide. Several studies have indicated that chronic inflammation is associated with an increased risk of several types of human cancer. The lung is vulnerable to various chemical and biological insults, and persistent exposure to these factors may result in the release of several inflammatory cytokines from inflammatory cells, thus leading to chronic inflammation and a risk of lung cancer. Due to the extensive application of C57BL/6J mice in lipid metabolism‑related research, it appears important to establish a lung cancer model based on C57BL/6J mice. Therefore, the present study designed an experimental model, in which C57BL/6J mice received several injections of urethane. The study aimed to explore whether inflammation has a role in this model of lung cancer. The results demonstrated that 10 weekly intraperitoneal injections of urethane induced a 100% lung tumor incidence, and urethane‑treated mice possessed higher numbers of immune cells. In addition, the expression levels of cytokines and chemokines in bronchoalveolar lavage fluid were significantly different between the two groups. Activation of the transcription factor nuclear factor‑κB was increased in the lung tissues of urethane‑treated mice, and its expression was upregulated in a time‑dependent manner. These results suggested that the accumulation of lung inflammation may be associated with the occurrence of lung cancer in C57BL/6J mice.
authors: School of Public Health and Management, Research Center for Medicine and Social Development, Innovation Center for Social Risk Governance in Health, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Cai
authors: C
authors: Xu
authors: School of Public Health and Management, Research Center for Medicine and Social Development, Innovation Center for Social Risk Governance in Health, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Lingyu
authors: L
authors: Zhou
authors: School of Public Health and Management, Research Center for Medicine and Social Development, Innovation Center for Social Risk Governance in Health, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Lei
authors: L
authors: Lu
authors: Department of Prevention and Control of Chronic Diseases, Center for Disease Control and Prevention in Chongqing, Chongqing 400016, P.R. China.
authors: Ting
authors: T
authors: Chen
authors: School of Public Health and Management, Research Center for Medicine and Social Development, Innovation Center for Social Risk Governance in Health, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Siyu
authors: S
authors: Wei
authors: School of Public Health and Management, Research Center for Medicine and Social Development, Innovation Center for Social Risk Governance in Health, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Xiaojing
authors: X
authors: Lin
authors: School of Public Health and Management, Research Center for Medicine and Social Development, Innovation Center for Social Risk Governance in Health, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Xuemei
authors: X
authors: Lian
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001992:Bronchoalveolar Lavage Fluid; D002273:Carcinogens; D004195:Disease Models, Animal; D006801:Humans; D007249:Inflammation / Q000139:chemically induced* / Q000150:complications / Q000276:immunology / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology*; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000209:etiology / Q000276:immunology / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000032:analysis* / Q000276:immunology; D014520:Urethane
chemical_list: D002273:Carcinogens; D016328:NF-kappa B; D014520:Urethane