title: A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool.
abstract: Directed evolution was used to improve the activity of JamB, a membrane-bound bifunctional desaturase/acetylenase. To quickly assess the protein engineering outcomes, we developed a new platform for quantifying extracellular alkyne-tagged metabolites through a fluorogenic click reaction. Random mutagenesis yielded the best JamB variant with ∼20-fold increased activity in E. coli.
authors: Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94704, USA.
authors: Xuejun
authors: X
authors: Zhu
authors: Peyton
authors: P
authors: Shieh
authors: Michael
authors: M
authors: Su
authors: Carolyn R
authors: CR
authors: Bertozzi
authors: Wenjun
authors: W
authors: Zhang