title: Association ofC677T MTHFRandG20210A FIIprothrombin polymorphisms with susceptibility to myocardial infarction.
abstract: Myocardial infarction (MI) is a common complex pathology, localized in the main leading causes of mortality worldwide. It is the result of the interaction of genetic and environmental factors. The aim of the present study was to investigate the potential association of C677T 5,10-methylenetetrahydrofolate reductase (MTHFR) (rs1801133) andG20210Afactor II prothrombin (FII) (rs1799963) polymorphisms with the susceptibility of MI. Following extraction by the standard salting-out procedure, DNA samples of 100 MI patients and 182 apparently healthy controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism usingHinfI andHindIII restriction enzymes, respectively. The results show a significant association of theG20210T FIIpolymorphism with the MI risk. The frequencies of the heterozygote genotype GA, homozygous mutated AA and theG20210Aallele was higher among patients compared to controls (GA: 59 vs. 5.5%, P<0.001; AA: 10 vs. 0%, P=0.003; and 20210A: 39.5 vs. 2.7%, P<0.003), suggesting that this polymorphism may be a potential genetic marker for MI. No significant association was observed between theC677T MTHFRand MI occurrence, and there was more heterozygote CT in the patient group compared to the controls. As a multifactorial disease, the development of MI may be the result of numerous factors that influence synergistically its occurrence. Thus, further studies are merited to try to better assess these associations (gene-gene and gene-environment interactions).
authors: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca BP 9154, Morocco.
authors: Wiam
authors: W
authors: Hmimech
authors: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca BP 9154, Morocco.
authors: Hind Hassani
authors: HH
authors: Idrissi
authors: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca BP 9154, Morocco.
authors: Brehima
authors: B
authors: Diakite
authors: Department of Cardiology, Ibn Rochd University Hospital Center, Casablanca 20102, Morocco.
authors: Dalila
authors: D
authors: Baghdadi
authors: Department of Cardiology, Ibn Rochd University Hospital Center, Casablanca 20102, Morocco.
authors: Farah
authors: F
authors: Korchi
authors: Department of Cardiology, Ibn Rochd University Hospital Center, Casablanca 20102, Morocco.
authors: Rachida
authors: R
authors: Habbal
authors: Laboratory of Genetics and Molecular Pathology, Medical School, University Hassan II, Casablanca BP 9154, Morocco.
authors: Sellama
authors: S
authors: Nadifi
keywords: C677T MTHFR; G20210A FII; Moroccan population; association; myocardial infarction; susceptibility