title: A Vernonia Diacylglycerol Acyltransferase Can Increase Renewable Oil Production.
abstract: Increasing the production of plant oils such as soybean oil as a renewable resource for food and fuel is valuable. Successful breeding for higher oil levels in soybean, however, usually results in reduced protein, a second valuable seed component. This study shows that by manipulating a highly active acyl-CoA:diacylglycerol acyltransferase (DGAT) the hydrocarbon flux to oil in oilseeds can be increased without reducing the protein component. Compared to other plant DGATs, a DGAT from Vernonia galamensis (VgDGAT1A) produces much higher oil synthesis and accumulation activity in yeast, insect cells, and soybean. Soybean lines expressing VgDGAT1A show a 4% increase in oil content without reductions in seed protein contents or yield per unit land area. Incorporation of this trait into 50% of soybeans worldwide could result in an increase of 850 million kg oil/year without new land use or inputs and be worth ∼U.S.$1 billion/year at 2012 production and market prices.
authors: Department of Bioresource Science, Kobe University , Kobe, Japan.
authors: Tomoko
authors: T
authors: Hatanaka
authors: Department of Plant and Soil Sciences, University of Kentucky , Lexington, Kentucky, United States.
authors: William
authors: W
authors: Serson
authors: Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University , Taigu, China.
authors: Runzhi
authors: R
authors: Li
authors: USDA-ARS, EWERU-CGHAR , Manhattan, Kansas, United States.
authors: Paul
authors: P
authors: Armstrong
authors: Department of Plant and Soil Sciences, University of Kentucky , Lexington, Kentucky, United States.
authors: Keshun
authors: K
authors: Yu
authors: Department of Plant and Soil Sciences, University of Kentucky , Lexington, Kentucky, United States.
authors: Todd
authors: T
authors: Pfeiffer
authors: Department of Plant and Soil Sciences, University of Kentucky , Lexington, Kentucky, United States.
authors: Xi-Le
authors: XL
authors: Li
authors: Department of Plant and Soil Sciences, University of Kentucky , Lexington, Kentucky, United States.
authors: David
authors: D
authors: Hildebrand
mesh_terms: D003001:Cloning, Molecular; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010938:Plant Oils / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics; D012639:Seeds / Q000737:chemistry / Q000235:genetics; D013025:Glycine max / Q000737:chemistry / Q000235:genetics; D035741:Vernonia / Q000201:enzymology* / Q000235:genetics
chemical_list: D005227:Fatty Acids; D010938:Plant Oils; D010940:Plant Proteins; D051048:Diacylglycerol O-Acyltransferase
keywords: Glycine max; Vernonia galamensis; diacylglycerol acyltransferase; lipids; oilseeds; triacylglycerol