title: In Vitro Cultivation of Primary Prostate Cancer Cells Alters the Molecular Biomarker Pattern.
abstract: OBJECTIVE
  The high variability of primary cells propagated in vitro led us to study the expression patterns of 11 most commonly accepted and widely used biomarkers specific for prostate cancer (PC) cells in primary cell models.
  
  
  METHODS
  Primary PC cells from five PC patients were partially subjected to RNA preparation immediately and remaining cells were propagated up to 84 days followed by RNA preparation. Subsequently, biomarker mRNA quantification was performed by quantitative reverse transcription-polymerase chain reaction (RT-PCR) and biomarker transcript concentrations before and after cultivation of primary PC cells were compared.
  
  
  RESULTS
  Evaluation of androgen receptor, prostate-specific antigen, acid phosphatase, prostate-specific membrane antigen, fatty acid synthase, cytokeratin types 5/8/19, E-cadherin, epithelial cell adhesion molecule and fibroblast-specific protein 1 demonstrated temporal changes, as well as individual differences in expression, during primary PC cell propagation.
  
  
  CONCLUSIONS
  Experimental design, as well as data evaluation, may need to take under consideration the high variability of biomarker expression in primary PC cells.
authors: Department of Urology, University Medicine Greifswald, Greifswald, Germany.
authors: Anne
authors: A
authors: Caspar
authors: Competence Center Functional Genomics, Junior Research Group Pathoproteomics, University of Greifswald, Greifswald, Germany.
authors: Jörg
authors: J
authors: Mostertz
authors: Department of Urology, University Medicine Greifswald, Greifswald, Germany.
authors: Merle
authors: M
authors: Leymann
authors: Institute of Occupational and Social Medicine, RWTH Aachen Universtity, Aachen, Germany.
authors: Patrick
authors: P
authors: Ziegler
authors: Department of Pathology, University Regensburg, Regensburg, Germany Department of Pathology, University Medicine Greifswald, Greifswald, Germany.
authors: Katja
authors: K
authors: Evert
authors: Department of Pathology, University Regensburg, Regensburg, Germany Department of Pathology, University Medicine Greifswald, Greifswald, Germany.
authors: Matthias
authors: M
authors: Evert
authors: Department of Urology, University Medicine Greifswald, Greifswald, Germany.
authors: Uwe
authors: U
authors: Zimmermann
authors: Department of Anatomy and Cell Biology, RWTH Aachen Universtity, Aachen, Germany.
authors: Lars-Ove
authors: LO
authors: Brandenburg
authors: Department of Urology, University Medicine Greifswald, Greifswald, Germany.
authors: Martin
authors: M
authors: Burchardt
authors: Department of Urology, University Medicine Greifswald, Greifswald, Germany matthias.stope@uni-greifswald.de.
authors: Matthias B
authors: MB
authors: Stope
mesh_terms: D015703:Antigens, CD; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics; D000071858:Epithelial Cell Adhesion Molecule / Q000096:biosynthesis / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D043425:Glutamate Carboxypeptidase II / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008297:Male; D061251:Primary Cell Culture; D017430:Prostate-Specific Antigen / Q000096:biosynthesis / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D011944:Receptors, Androgen / Q000096:biosynthesis / Q000235:genetics
chemical_list: C410798:AR protein, human; D015703:Antigens, CD; D000954:Antigens, Surface; D014408:Biomarkers, Tumor; C513384:CDH1 protein, human; D015820:Cadherins; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D012333:RNA, Messenger; D011944:Receptors, Androgen; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II; D017430:Prostate-Specific Antigen
keywords: Prostate cancer; biomarker; primary cell culture