title: Synthetic assembly of novel avidin-biotin-GlcNAc (ABG) complex as an attractive bio-probe and its interaction with wheat germ agglutinin (WGA).
abstract: A tetravalent GlcNAc pendant glycocluster was constructed with terminal biotin through C6 linker. To acquire the multivalent carbohydrate-protein interactions, we synthesized a glycopolymer of tetrameric structure using N-acetyl-d-glucosamine (GlcNAc) as the target carbohydrate by the use of 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) as coupling reagent, followed by biotin-avidin complexation leading to the formation of glycocluster of avidin-biotin-GlcNAc conjugate (ABG complex). The dynamic light scattering (DLS) system was implied for size detection and to check the binding affinity of GlcNAc conjugate with a WGA lectin we use fluorometric assay by means of specific excitation of tryptophan at λex 295nm and it was found to be very high Ka∼1.39×10(7) M(-1) in case of ABG complex as compared to GlcNAc only Ka∼1.01×10(4) M(-1) with the phenomenon proven to be due to glycocluster effect.
authors: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan.
authors: Amrita
authors: A
authors: Kumari
authors: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan.
authors: Tetsuo
authors: T
authors: Koyama
authors: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan.
authors: Ken
authors: K
authors: Hatano
authors: Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan. Electronic address: koji@fms.saitama-u.ac.jp.
authors: Koji
authors: K
authors: Matsuoka
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry*; D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D008968:Molecular Conformation; D014909:Wheat Germ Agglutinins / Q000737:chemistry*
chemical_list: D014909:Wheat Germ Agglutinins; D001360:Avidin; D001710:Biotin; D000117:Acetylglucosamine
keywords: Bio-probe; Biotin; Glycocluster; Lectin; Multivalency; Tryptophan