title: Development for the measurement of serum thiosulfate using LC-MS/MS in forensic diagnosis of H2S poisoning.
abstract: Thiosulfate measurement is crucial to diagnosis of hydrogen sulfide (H2S) poisoning in forensic toxicology. Although GC-MS method is currently regarded as a standard thiosulfate measurement, it requires complicated sample preparation prior to analysis. This study presents a simple, rapid, and highly sensitive method for the quantitative analysis of serum thiosulfate by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). This method is based on selected reaction monitoring and has high sensitivity with a lower quantification limit of 0.5μM. Precision and accuracy of this method meet the basic requirements for quantitative analysis (intra- and inter-day tests have a relative standard deviation of ⩽10.4%; range of analytical recovery is 94.3-102.6%). On the measurements of serum thiosulfate by our developed method, a thiosulfate concentration as 57.5μM was detected clearly in the H2S poisoning case comparing to the non poisoning case in which only a trace amount of thiosulfate was observed.
authors: Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
authors: Shigeki
authors: S
authors: Jin
authors: Department of Forensic Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan; Center for Cause of Death Investigation, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors: Hideki
authors: H
authors: Hyodoh
authors: Department of Forensic Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan; Center for Cause of Death Investigation, Hokkaido University Graduate School of Medicine, Sapporo, Japan. Electronic address: k-matoba@med.hokudai.ac.jp.
authors: Kotaro
authors: K
authors: Matoba
authors: Department of Forensic Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors: Fei
authors: F
authors: Feng
authors: Tokyo Medical Examiner's Office, Tokyo Metropolitan Government, Tokyo, Japan.
authors: Akira
authors: A
authors: Hayakawa
authors: Department of Forensic Medicine, Asahikawa Medical University, Asahikawa, Japan.
authors: Katsuhiro
authors: K
authors: Okuda
authors: Department of Forensic Medicine, Asahikawa Medical University, Asahikawa, Japan.
authors: Keiko
authors: K
authors: Shimizu
authors: Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
authors: Sanae
authors: S
authors: Haga
authors: Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.
authors: Michitaka
authors: M
authors: Ozaki
authors: Department of Forensic Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors: Koichi
authors: K
authors: Terazawa
mesh_terms: D000328:Adult; D001344:Autopsy; D002853:Chromatography, Liquid / Q000379:methods*; D053593:Forensic Toxicology; D006801:Humans; D006862:Hydrogen Sulfide / Q000506:poisoning*; D008297:Male; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013885:Thiosulfates / Q000097:blood*
chemical_list: D013885:Thiosulfates; D006862:Hydrogen Sulfide
keywords: Forensic toxicology; Hydrogen sulfide poisoning; Liquid chromatography–tandem mass spectrometry; Thiosulfate