title: Effects of luteolin and quercetin 3-β-d-glucoside identified from Passiflora subpeltata leaves against acetaminophen induced hepatotoxicity in rats.
abstract: Passiflora subpeltata has many beneficial effects in the treatment of various diseases including inflammation, pain and fever. This study was aimed to analyze the phytochemical compounds present in acetone extract of P. subpeltata leaves and to evaluate their performance against paracetamol induced hepatotoxicity activity. HPLC-DAD method was used to identify and quantify the phytochemical compounds. Hepatoprotective activity of acetone extract in the treatment of rat liver functions was monitored by the measurement of blood parameters and serum biochemical parameters such as SGOT, SGPT, ALP and in vivo antioxidant parameters viz. SOD, CAT and LPO. Further, liver tissues were also subjected to histopathological analysis. The HPLC-DAD results showed the luteolin and quercetin 3-β-d-glucoside as newly identified compounds in P. subpeltata species. Pre-treatment with acetone extract of P. subpeltata leaves at 200 and 400mg/kg doses significantly elevated the WBC, RBC and HB counts and retained the serum biochemical and enzymatic antioxidants levels to normal level. Based on this detailed study we conclude that acetone extract of P. subpeltata leaves offered better protection against hepatotoxicity induced by the acetaminophen.
authors: Department of Pharmacy, Federal University of Sergipe, CEP 49100-000, Sao Cristovao, Brazil. Electronic address: saranflora04@gmail.com.
authors: Saravanan
authors: S
authors: Shanmugam
authors: Bioprospecting laboratory, Department of Botany, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India. Electronic address: drparimel@gmail.com.
authors: Parimelazhagan
authors: P
authors: Thangaraj
authors: Department of Pharmacy, Federal University of Sergipe, CEP 49100-000, Sao Cristovao, Brazil.
authors: Bruno Dos Santos
authors: BDS
authors: Lima
authors: Bioprospecting laboratory, Department of Botany, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India.
authors: Rahul
authors: R
authors: Chandran
authors: Department of Pharmacy, Federal University of Sergipe, CEP 49100-000, Sao Cristovao, Brazil.
authors: Adriano Antunes
authors: AA
authors: de Souza Araújo
authors: Laboratory of Flavor and Chromatographic Analysis, Federal University of Sergipe, CEP 49100-000, Sao Cristovao, Brazil.
authors: Narendra
authors: N
authors: Narain
authors: Department of Pharmacy, Federal University of Sergipe, CEP 49100-000, Sao Cristovao, Brazil.
authors: Mairim Russo
authors: MR
authors: Serafini
authors: Department of Pharmacy, Federal University of Sergipe, CEP 49100-000, Sao Cristovao, Brazil.
authors: Lucindo José Quintans
authors: LJQ
authors: Júnior
mesh_terms: D000082:Acetaminophen / Q000633:toxicity*; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D047311:Luteolin / Q000302:isolation & purification / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D029598:Passiflora; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D018515:Plant Leaves; D011794:Quercetin / Q000302:isolation & purification / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D010936:Plant Extracts; D000082:Acetaminophen; D011794:Quercetin; D047311:Luteolin
keywords: Acetaminophen; Hepatoprotective; Luteolin; Passiflora subpeltata; Quercetin 3-β-d-glucoside