title: Developing a biomaterial interface based on poly(lactic acid) via plasma-assisted covalent anchorage of d-glucosamine and its potential for tissue regeneration.
abstract: The aim of this study was to develop the potential tissue engineering applications of d-glucosamine (GlcN) immobilized onto the surface of a biodegradable matrix in order to induce a desired biological effect at biointerfaces. Thus, for sample preparation we used a novel multistep physicochemical approach. In the first step the poly(lactic acid) (PLA) films were exposed to a low pressure plasma in air atmosphere, followed by radical graft copolymerization with acrylic acid to yield a carboxyl-functionalized spacer layer on the PLA surface. The carboxyl groups were then coupled to GlcN molecules via the carbodiimide chemistry. The developed surfaces were characterized by X-ray Photoelectron Spectroscopy (XPS), Contact angle measurements and Atomic Force Microscopy (AFM). A preliminary study on the proliferation of fibroblasts on the developed surfaces was performed using the NIH/3T3 cell line.
authors: Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic; Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo 11566, Egypt.
authors: Ahmed E
authors: AE
authors: Swilem
authors: Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic. Electronic address: lehocky@post.cz.
authors: Marian
authors: M
authors: Lehocký
authors: Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic.
authors: Petr
authors: P
authors: Humpolíček
authors: Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic.
authors: Zdenka
authors: Z
authors: Kucekova
authors: Department of Surface Engineering and Optoelectronics, Jozef Stefan Institute, Jamova Cesta 39, 1000 Ljubljana, Slovenia.
authors: Ita
authors: I
authors: Junkar
authors: Department of Surface Engineering and Optoelectronics, Jozef Stefan Institute, Jamova Cesta 39, 1000 Ljubljana, Slovenia.
authors: Miran
authors: M
authors: Mozetič
authors: Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo 11566, Egypt.
authors: Ashraf A
authors: AA
authors: Hamed
authors: Polymer Institute, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 41 Bratislava, Slovakia.
authors: Igor
authors: I
authors: Novák
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D049109:Cell Proliferation; D005944:Glucosamine / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000737:chemistry*; D012038:Regeneration; D013499:Surface Properties; D023822:Tissue Engineering; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C033616:poly(lactide); D005944:Glucosamine
keywords: Carbodiimide; Fibroblast viability; Plasma treatment; Poly(lactic acid); d-Glucosamine