title: Dihydromyricetin from Ampelopsis grossedentata inhibits melanogenesis through down-regulation of MAPK, PKA and PKC signaling pathways.
abstract: The inhibitory effects of dihydromyricetin purified from Ampelopsis grossedentata on melanogenesis and its antioxidant characteristics were investigated. Assays of tyrosinase activities and melanin content in B16F10 mouse melanoma cells were carried out spectrophotometrically, and the expression of melanogenesis-related proteins was determined by Western blotting. The possible signaling pathways involved in dihydromyricetin-mediated depigmentation were also examined using specific protein kinase regulators. The results revealed that dihydromyricetin effectively suppresses intracellular tyrosinase activity and decreases melanin amount in cells. Dihydromyricetin also exhibits antioxidant properties and effectively decreases intracellular reactive oxygen species (ROS) and reactive species (RS) levels. Our results indicated that dihydromyricetin inhibits melanogenesis through its antioxidant properties and by downregulating protein kinase A (PKA), protein kinase C (PKC), and mitogen-activated protein kinases (MAPK) signaling pathways. The present study indicates that dihydromyricetin has the potential to be developed into a depigmentation skin care product.
authors: Department of Medical Laboratory Science and Biotechnology, China Medical University, No 91 Hsueh-Shih Road, Taichung, 40402, Taiwan. Electronic address: lchuang@mail.cmu.edu.tw.
authors: Huey-Chun
authors: HC
authors: Huang
authors: Department of Applied Cosmetology & Master Program of Cosmetic Sciences, Hungkuang University, No. 1018, Section 6, Taiwan Boulevard, Shalu District, Taichung, 43302, Taiwan. Electronic address: ellis052279@yahoo.com.tw.
authors: Chun-Chieh
authors: CC
authors: Liao
authors: Department of Applied Cosmetology & Master Program of Cosmetic Sciences, Hungkuang University, No. 1018, Section 6, Taiwan Boulevard, Shalu District, Taichung, 43302, Taiwan. Electronic address: chuchun0228@yahoo.com.tw.
authors: Chu-Chun
authors: CC
authors: Peng
authors: Department of Applied Cosmetology & Master Program of Cosmetic Sciences, Hungkuang University, No. 1018, Section 6, Taiwan Boulevard, Shalu District, Taichung, 43302, Taiwan. Electronic address: jiajia2008@hotmail.my.
authors: Jia-Min
authors: JM
authors: Lim
authors: Department of Applied Cosmetology & Master Program of Cosmetic Sciences, Hungkuang University, No. 1018, Section 6, Taiwan Boulevard, Shalu District, Taichung, 43302, Taiwan. Electronic address: crazybone917@yahoo.com.tw.
authors: Jen-Hung
authors: JH
authors: Siao
authors: Department of Applied Cosmetology & Master Program of Cosmetic Sciences, Hungkuang University, No. 1018, Section 6, Taiwan Boulevard, Shalu District, Taichung, 43302, Taiwan. Electronic address: flead7889@sunrise.hk.edu.tw.
authors: Chien-Mei
authors: CM
authors: Wei
authors: Department of Biotechnology, Hungkuang University, No. 1018, Section 6, Taiwan Boulevard, Shalu District, Taichung, 43302, Taiwan. Electronic address: ccchen@sunrise.hk.edu.tw.
authors: Chien-Chih
authors: CC
authors: Chen
authors: Department of Family Medicine, Kuang Tien General Hospital, Taichung, Taiwan. Electronic address: c686ab@yahoo.com.tw.
authors: Chung-Shing
authors: CS
authors: Wu
authors: Department of Applied Cosmetology & Master Program of Cosmetic Sciences, Hungkuang University, No. 1018, Section 6, Taiwan Boulevard, Shalu District, Taichung, 43302, Taiwan. Electronic address: ctm@sunrise.hk.edu.tw.
authors: Tsong-Min
authors: TM
authors: Chang
mesh_terms: D032421:Ampelopsis / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D045744:Cell Line, Tumor; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects*; D044948:Flavonols / Q000737:chemistry / Q000494:pharmacology*; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D044948:Flavonols; D008543:Melanins; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D020928:Mitogen-Activated Protein Kinases; C472036:dihydromyricetin
keywords: Ampelopsis grossedentata; Dihydromyricetin; Melanin; Melanogenesis; Tyrosinase