title: Effect of bone marrow and adipose tissue-derived mesenchymal stem cells on the natural course of corneal scarring after penetrating injury.
abstract: In the present study, we investigate and compare the efficacy of bone marrow- and adipose tissue-derived mesenchymal stem cell (MSCs) in corneal wound healing. A penetrating injury was created in the right corneas of Wistar rats (n = 40). Ten microliters of phosphate-buffered solution (PBS) containing 2 × 10(5) green fluorescent protein (GFP) labeled bone-marrow-derived MSCs to group 1 (n = 15), 10 μl of PBS containing 2 × 10(5) GFP-labeled adipose-tissue-derived MSCs to group 2 (n = 15), 10 μl PBS was injected into anterior chamber in group 3 (n = 10, control). Corneal opacity scoring, in vivo confocal microscopy, and histopathological evaluation were done at the end of 8 weeks. Immunofluorescence sections were evaluated to detect transplanted cells. Immune staining was performed to measure the expression levels of keratocan, aldehyde dehydrogenase (ALDH) and CD34. The gene expression levels of tumor necrosis factor (TNF-α), the interleukin 6 receptor (IL-6R), interleukin 12b (IL-12b), and transforming growth factor beta (TGF-β1) was measured on corneas. The establishment of stem cells in the corneas of the transplanted groups was confirmed by immunofluorescence staining. The expression of keratocan, ALDH, and CD34 increased in the transplanted groups (p < 0.05). The density of keratocytes increased significantly in both transplanted groups according to the in vivo confocal microscopy data (p < 0.05). The expression of TNF-α, IL-6R, and IL-12b decreased significantly in the transplanted groups (p < 0.05). Based on our findings, we consider that allogeneic stem cells facilitate the regeneration of corneal stroma and can be a cell source for stromal repopulation in diseased cornea.
authors: Department of Ophthalmology, Bakirkoy Dr. Sadi Konuk Education and Research Hospital, Istanbul, Turkey. Electronic address: bengiyucel@hotmail.com.
authors: Bengi
authors: B
authors: Demirayak
authors: Department of Ophthalmology, School of Medicine, Kocaeli University, Kocaeli, Turkey.
authors: Nurşen
authors: N
authors: Yüksel
authors: Department of Ophthalmology, School of Medicine, Kocaeli University, Kocaeli, Turkey.
authors: Onur Sinan
authors: OS
authors: Çelik
authors: Center for Regenerative Medicine and Stem Cell Research and Manufacturing, Liv Hospital, Istanbul, Turkey.
authors: Cansu
authors: C
authors: Subaşı
authors: Center for Stem Cell and Gene Therapies Research and Practice, Department of Stem Cell, Institute of Health Sciences, Kocaeli University, Kocaeli, Turkey.
authors: Gökhan
authors: G
authors: Duruksu
authors: Center for Stem Cell and Gene Therapies Research and Practice, Department of Stem Cell, Institute of Health Sciences, Kocaeli University, Kocaeli, Turkey.
authors: Z Seda
authors: ZS
authors: Unal
authors: Department of Pathology, School of Medicine, Kocaeli University, Kocaeli, Turkey.
authors: Demir Kürşat
authors: DK
authors: Yıldız
authors: Center for Regenerative Medicine and Stem Cell Research and Manufacturing, Liv Hospital, Istanbul, Turkey; Department of Histology and Embryology, Faculty of Medicine, Istinye University, Turkey.
authors: Erdal
authors: E
authors: Karaöz
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000637:transplantation; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002478:Cells, Cultured; D002921:Cicatrix / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D065306:Corneal Injuries / Q000150:complications / Q000473:pathology / Q000601:surgery*; D003319:Corneal Stroma / Q000293:injuries / Q000473:pathology; D004195:Disease Models, Animal; D015807:Eye Injuries, Penetrating / Q000150:complications / Q000473:pathology / Q000601:surgery*; D005260:Female; D005434:Flow Cytometry; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018613:Microscopy, Confocal; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing
keywords: Cornea; Keratocan; Mesenchymal stem cells; Stromal wound healing