title: MicroRNA-206 Inhibited the Progression of Glioblastoma Through BCL-2.
abstract: Gliomas are the most common type of brain tumor and have a poor prognosis. MicroRNAs (miRNAs) are a class of small, endogenous, and non-coding RNAs that play crucial roles in cell proliferation, survival, and invasion. Deregulated expression of miR-206 has been investigated in many cancers. However, the role of miR-206 in glioblastoma is still unclear. In the present study, we found that the expression of miR-206 was decreased in cancer tissues compared with normal tissues. However, the expression level of BCL-2 was higher in cancer tissues than that in normal tissues (all p < 0.001). Statistically, the expression level of BCL-2 was inversely correlated with the miR-206. In addition, the overall survival of glioblastoma patients with lower miR-206 expression was significantly shorter than those with high miR-206 expression (p < 0.001). Besides, the expression of miR-206 was also decreased in U87 and U251 cells. In vitro assays showed that ectopic miR-206 expression affected the proliferation, cell cycle, and invasion in U87 and U251 cells. Importantly, we identified BCL-2 as a direct target of miR-206 in U87 and U251 cells using luciferase assay. Overexpression of BCL-2 partially attenuated the miR-206-mediated cell proliferation. In vivo, overexpression of miR-206 suppressed the progression of glioblastoma cells using mice xenograft model. In conclusion, this study suggested that miR-206 could act as a tumor suppressor gene through inhibiting BCL-2 in the development of glioblastoma.
authors: Department of Neurosurgery, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.|Department of Neurosurgery, Affiliated Hospital, Medical College of Yan'an University, Yan'an City, Shaanxi, 716000, People's Republic of China.
authors: Wenjiong
authors: W
authors: Hao
authors: Department of Neurosurgery, The Affiliated Hospital of Shaanxi Traditional Chinese Medicine University, Xianyang, 712000, People's Republic of China.
authors: Wei
authors: W
authors: Luo
authors: Department of Neurosurgery, Affiliated Hospital, Medical College of Yan'an University, Yan'an City, Shaanxi, 716000, People's Republic of China.
authors: Mangmang
authors: M
authors: Bai
authors: Department of Neurosurgery, Affiliated Hospital, Medical College of Yan'an University, Yan'an City, Shaanxi, 716000, People's Republic of China.
authors: Jian
authors: J
authors: Li
authors: Department of Neurosurgery, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.
authors: Xiaobin
authors: X
authors: Bai
authors: Department of Neurosurgery, Affiliated Hospital, Medical College of Yan'an University, Yan'an City, Shaanxi, 716000, People's Republic of China.
authors: Jie
authors: J
authors: Guo
authors: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, 200040, People's Republic of China.
authors: Jinsong
authors: J
authors: Wu
authors: Department of Neurosurgery, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China. maodewang@163.com.
authors: Maode
authors: M
authors: Wang
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000378:metabolism
chemical_list: C546087:MIRN206 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2
keywords: BCL-2; Glioblastoma; Suppressor; miR-206