title: Metabolic Labeling in the Study of Mammalian Ribosomal RNA Synthesis.
abstract: RNA metabolic labeling is a method of choice in the study of dynamic changes in the rate of gene transcription and RNA processing. It is particularly applicable to transcription of the ribosomal RNA genes and their processing products due to the very high levels of ribosomal RNA synthesis. Metabolic labeling can detect changes in ribosomal RNA transcription that occur within a few minutes as opposed to the still widely used RT-PCR or Northern blot procedures that measure RNA pool sizes and at best are able to detect changes occurring over several hours or several days. Here, we describe a metabolic labeling technique applicable to the measurement of ribosomal RNA synthesis and processing rates, as well as to the determination of RNA Polymerase I transcription elongation rates.
authors: Laboratory of Growth and Development, St-Patrick Research Group in Basic Oncology, Cancer Division of the Quebec University Hospital Research Centre, Edifice St Patrick, 9 rue McMahon, Québec, QC, Canada, G1R 3S3.|Department of Molecular Biology, Medical Biochemistry and Pathology, Faculty of Medicine, Laval University, Edifice St Patrick, 9 rue McMahon, Québec, QC, Canada, G1R 3S3.
authors: Victor Y
authors: VY
authors: Stefanovsky
authors: Laboratory of Growth and Development, St-Patrick Research Group in Basic Oncology, Cancer Division of the Quebec University Hospital Research Centre, Edifice St Patrick, 9 rue McMahon, Québec, QC, Canada, G1R 3S3. tom.moss@crhdq.ulaval.ca.|Department of Molecular Biology, Medical Biochemistry and Pathology, Faculty of Medicine, Laval University, Edifice St Patrick, 9 rue McMahon, Québec, QC, Canada, G1R 3S3. tom.moss@crhdq.ulaval.ca.
authors: Tom
authors: T
authors: Moss
mesh_terms: D000818:Animals; D004587:Electrophoresis, Agar Gel; D008322:Mammals / Q000235:genetics / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D012318:RNA Polymerase I / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D013194:Staining and Labeling; D061805:Transcription Elongation, Genetic; D014158:Transcription, Genetic
chemical_list: D012322:RNA Precursors; D012335:RNA, Ribosomal; D012318:RNA Polymerase I
keywords: Electrophoresis; Fluorography; Metabolic labeling; Phospho-imaging; Precursor rRNA; RNA polymerase I (RPI or PolI); Ribosomal RNA (rRNA); Scintillation counting; Transcription elongation; Vacuum transfer of RNA; rRNA processing rate; rRNA synthesis rate