title: Study of differentiated human umbilical cord-derived mesenchymal stem cells transplantation on rat model of advanced parkinsonism.
abstract: The aim of this study was to explore the curative effect of differentiated human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) transplantation on rat of advanced Parkinson disease (PD) model. Human umbilical cord-derived mesenchymal stem cells were cultured and induced differentiation in vitro. The PD rats were established and allocated randomly into 2 groups: differentiated hUC-MSCs groups and physiological saline groups (the control group). Rotation test and immunofluorescence double staining were done. The result showed that hUC-MSCs could differentiate into mature dopamine neurons. Frequency of rotation was significantly less in differentiated hUC-MSCs groups than in normal saline group. After we transplanted these cells into the unilateral lesioned substantia nigra induced by striatal injection of 6-hydroxydopamine and performed in the medial forebrain bundle and ventral tegmental area, nigral tyrosine hydroxylase-positive cells were observed and survival of at least 2 months. In addition, transplantation of hUC-MSCs could make an obviously therapeutic effect on PD rats.
authors: Dept of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, P. R .C;
authors: Zhaowei
authors: Z
authors: Wang
authors: Qianjiang Central Hospital, Qianjiang 433100, Hubei Province, P. R .C.
authors: Aimin
authors: A
authors: Chen
authors: Qianjiang Central Hospital, Qianjiang 433100, Hubei Province, P. R .C.
authors: Shengjuan
authors: S
authors: Yan
authors: Dept of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, P. R .C;
authors: Chengyan
authors: C
authors: Li
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D017072:Neostriatum / Q000378:metabolism / Q000473:pathology; D020734:Parkinsonian Disorders / Q000473:pathology / Q000628:therapy*; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D015415:Biomarkers; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine
keywords: Parkinson disease; human umbilical cord-derived mesenchymal stem cells; transplantation; tyrosine hydroxylase