title: Anti-inflammatory activities of Dangyuja (Citrus grandis Osbeck) in concanavalin A stimulated murine splenocytes and 12-O-tetradecanoylphorbol-13-acetate-induced murine skin edema.
abstract: Dangyuja (Citrus grandis Osbeck), a citrus cultivated in southern Korea, has been used in traditional medicine for its anti-inflammatory effect. In this study, we investigated the anti-inflammatory potential of extract of Citrus grandis Osbeck (ECGO). In in vitro assays, ECGO treatment of concanavalin A (10μg/ml, for 24h) stimulated splenocytes showed significant reduction in CD44/CD62L+T cell population and a marked decrease in the production of inflammatory cytokines IL-2, IFN-γ and IL-4. Interestingly, in vivo assays of ECGO topical treatment (100μg/20μl/ear) significantly mitigated the TPA (4μg/20μl/ear) induced edema induction and Myeloperoxidase activity. Anti-inflammatory potential of ECGO were further evidenced through its potent decrease in expression of inducible nitric oxide, cyclooxygenase-2, IL-1β and TNF-α and suppressed homing of CD3+T cells and F4/80+macrophages to site of inflammation. This study emphasizes the possibility of developing ECGO as an alternative natural topical agent to combat inflammatory diseases.
authors: Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Republic of Korea. Electronic address: madushaniherath001@gmail.com.
authors: Kalahe Hewage Iresha Nadeeka Madushani
authors: KHINM
authors: Herath
authors: Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Republic of Korea. Electronic address: bsj814@naver.com.
authors: So Jin
authors: SJ
authors: Bing
authors: Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Republic of Korea. Electronic address: joejh89@nate.com.
authors: Jinhee
authors: J
authors: Cho
authors: Department of Advanced Convergence Technology & Science, Jeju National University, Jeju 63243, Republic of Korea. Electronic address: orange5687@naver.com.
authors: Areum
authors: A
authors: Kim
authors: Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Republic of Korea. Electronic address: alskdjf1991@naver.com.
authors: Sumin
authors: S
authors: Shin
authors: Jeju Diversity Research Institute, Jeju Technopark, Seogwipo 63608, Republic of Korea. Electronic address: kimgk350@jejutp.or.kr.
authors: Gi-Ok
authors: GO
authors: Kim
authors: Jeju Diversity Research Institute, Jeju Technopark, Seogwipo 63608, Republic of Korea. Electronic address: ljc0107@jejutp.or.kr.
authors: Jong-Chul
authors: JC
authors: Lee
authors: Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Republic of Korea; Department of Advanced Convergence Technology & Science, Jeju National University, Jeju 63243, Republic of Korea. Electronic address: yhjee@jejunu.ac.kr.
authors: Youngheun
authors: Y
authors: Jee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002957:Citrus; D003208:Concanavalin A / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D012871:Skin Diseases / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D003208:Concanavalin A; D013755:Tetradecanoylphorbol Acetate
keywords: Anti-inflammatory cytokines; COX-2; MPO activity; Skin inflammation; iNOS