title: Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats.
abstract: This study investigates morphological and biochemistry effects of binge ethanol consumption in parotid (PG) and submandibular (SG) salivary glands of rats from adolescence to adulthood. Female Wistar rats (n = 26) received ethanol at 3 g/kg/day (20% w/v) for 3 consecutive days/week from the 35th until the 62nd day of life. Animals were treated in two periods: 1 week (G1) and 4 weeks (G2), with a control (treated with distilled water) and an ethanol group to each period. In morphological analysis, morphometric and immunohistochemistry evaluation for smooth muscle actin (αSMA), cytokeratin-18 (CK-18), and vimentin (VIM) were made. Biochemical changes were analyzed by concentration of nitrites and levels of malondialdehyde (MDA). The difference between groups in each analysis was evaluated by Mann-Whitney U test or Student's t-test (p ≤ 0.05). PG showed, at one week of ethanol exposure, lower CK-18 and α-SMA expression, as well as MDA levels. After four weeks, lower CK-18 and higher MDA levels were observed in PG exposed to ethanol, in comparison to control group. SG showed lower α-SMA expression after 1 and 4 weeks of ethanol exposure as well as higher MDA levels after 1 week. Ethanol binge consumption during adolescence promotes tissue and biochemical changes with only one-week binge in acinar and myoepithelial PG cells.
authors: Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil.
authors: Nathalia Carolina Fernandes
authors: NC
authors: Fagundes
authors: Laboratory of Inflammation and Behaviour Pharmacology, Pharmacy Faculty, Institute of Health Science, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil.
authors: Luanna Melo Pereira
authors: LM
authors: Fernandes
authors: Laboratory of Molecular Pharmacology, Institute of Biological Sciences, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil.
authors: Ricardo Sousa de Oliveira
authors: RS
authors: Paraense
authors: Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil.
authors: Paulo Mecenas Alves
authors: 0000-0001-5051-7129
authors: PM
authors: de Farias-Junior
authors: Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil.
authors: Francisco Bruno
authors: FB
authors: Teixeira
authors: Department of Oral Pathology, School of Dentistry, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil.
authors: Sergio Melo
authors: SM
authors: Alves-Junior
authors: Department of Oral Pathology, School of Dentistry, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil.
authors: João de Jesus Viana
authors: Jde J
authors: Pinheiro
authors: Laboratory of Molecular Pharmacology, Institute of Biological Sciences, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil.
authors: Maria Elena
authors: ME
authors: Crespo-López
authors: Laboratory of Inflammation and Behaviour Pharmacology, Pharmacy Faculty, Institute of Health Science, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil.
authors: Cristiane Socorro Ferraz
authors: 0000-0003-4493-7375
authors: CS
authors: Maia
authors: Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Street Augusto Corrêa 1, Guamá, 66075-900 Belém, PA, Brazil.
authors: Rafael Rodrigues
authors: 0000-0003-1486-4013
authors: RR
authors: Lima
mesh_terms: D000199:Actins / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D063425:Binge Drinking / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D000067401:Blood Alcohol Content; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D053538:Keratin-18 / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D023421:Models, Animal; D009573:Nitrites / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010306:Parotid Gland / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D017208:Rats, Wistar; D013363:Submandibular Gland / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000066608:Underage Drinking; D014746:Vimentin / Q000378:metabolism
chemical_list: D000199:Actins; D015415:Biomarkers; D000067401:Blood Alcohol Content; D000431:Ethanol; D053538:Keratin-18; D008315:Malondialdehyde; D009573:Nitrites; D014746:Vimentin; C498862:Acta2 protein, rat