title: Calculations for reproducible autologous skin cell-spray grafting.
abstract: Non-cultured, autologous cell-spray grafting is an alternative to mesh grafting for larger partial- and deep partial-thickness burn wounds. The treatment uses a suspension of isolated cells, from a patient's donor site skin tissue, and cell-spray deposition onto the wound that facilitates re-epithelialization. Existing protocols for therapeutic autologous skin cell isolation and cell-spray grafting have defined the donor site area to treatment area ratio of 1:80, substantially exceeding the coverage of conventional mesh grafting. However, ratios of 1:100 are possible by maximizing the wound treatment area with harvested cells from a given donor site skin tissue according to a given burn area. Although cell isolation methods are very well described in the literature, a rational approach addressing critical aspects of these techniques are of interest in planning clinical study protocols. We considered in an experimental study the cell yield as a function of the donor site skin tissue, the cell density for spray grafting, the liquid spray volume, the sprayed distribution area, and the percentage of surface coverage. The experimental data was then used for the development of constants and mathematical equations to give a rationale for the cell isolation and cell-spray grafting processes and in planning for clinical studies.
authors: Bioreactor Group, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Roger
authors: R
authors: Esteban-Vives
authors: Bioreactor Group, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Matthew T
authors: MT
authors: Young
authors: The Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Toby
authors: T
authors: Zhu
authors: The Department of Bioengineering, Case Western Reserve University, Cleveland, OH, USA.
authors: Justin
authors: J
authors: Beiriger
authors: Bioreactor Group, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Chris
authors: C
authors: Pekor
authors: University of Pittsburgh Medical Center, UPMC Mercy Trauma and Burn Center, Pittsburgh, PA, USA.
authors: Jenny
authors: J
authors: Ziembicki
authors: University of Pittsburgh Medical Center, UPMC Mercy Trauma and Burn Center, Pittsburgh, PA, USA.
authors: Alain
authors: A
authors: Corcos
authors: The Plastic Surgery Department, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Peter
authors: P
authors: Rubin
authors: Bioreactor Group, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: jgerlach@pitt.edu.
authors: Jörg C
authors: JC
authors: Gerlach
mesh_terms: D001830:Body Surface Area; D002056:Burns / Q000628:therapy*; D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation; D017364:Collagenases; D010450:Endopeptidases; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000637:transplantation*; D016038:Skin Transplantation / Q000379:methods*; D060053:Transplant Donor Site; D014182:Transplantation, Autologous; D014357:Trypsin; D014945:Wound Healing
chemical_list: D010450:Endopeptidases; D014357:Trypsin; D017364:Collagenases; C023139:dispase
keywords: Burns; Cell-spray grafting; Equations; Keratinocytes; Wound healing