title: The role of Ly49E receptor expression on murine intraepithelial lymphocytes in intestinal cancer development and progression.
abstract: Ly49E is a member of the Ly49 family of NK receptors and is distinct from other members of this family on the basis of its structural properties, expression pattern and ligand recognition. Importantly, Ly49E receptor expression is high on small intestinal and colonic intraepithelial lymphocytes (IELs). Intestinal IELs are regulators of the mucosal immune system and contribute to front-line defense at the mucosal barrier, including anti-tumor immune response. Whereas most Ly49 receptors have MHC class-I ligands, we showed that Ly49E is instead triggered by urokinase plasminogen activator (uPA). uPA has been extensively implicated in tumor development, where increased uPA expression correlates with poor prognosis. As such, we investigated the role of Ly49E receptor expression on intestinal IELs in the anti-tumor immune response. For this purpose, we compared Ly49E wild-type mice to Ly49E knockout mice in two established tumor models: ApcMin/+-mediated and azoxymethane-induced intestinal cancer. Our results indicate that Ly49E expression on IELs does not influence the development or progression of intestinal cancer.
authors: Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.
authors: Aline
authors: 0000-0002-8347-1750
authors: A
authors: Van Acker
authors: Department of Rheumatology, Unit for Molecular Immunology and Inflammation, VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
authors: Els
authors: E
authors: Louagie
authors: Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.
authors: Jessica
authors: J
authors: Filtjens
authors: Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.
authors: Sylvie
authors: S
authors: Taveirne
authors: Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.
authors: Els
authors: E
authors: Van Ammel
authors: Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.
authors: Tessa
authors: T
authors: Kerre
authors: Department of Rheumatology, Unit for Molecular Immunology and Inflammation, VIB Inflammation Research Center, Ghent University, Ghent, Belgium.
authors: Dirk
authors: D
authors: Elewaut
authors: Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.
authors: Tom
authors: T
authors: Taghon
authors: Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.
authors: Bart
authors: B
authors: Vandekerckhove
authors: Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.
authors: Jean
authors: J
authors: Plum
authors: Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium. georges.leclercq@ugent.be.
authors: Georges
authors: G
authors: Leclercq
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics; D000818:Animals; D001397:Azoxymethane; D063646:Carcinogenesis; D002278:Carcinoma in Situ / Q000139:chemically induced / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D004848:Epithelium / Q000276:immunology* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D007111:Immunity, Cellular; D007414:Intestinal Neoplasms / Q000139:chemically induced / Q000235:genetics / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D055650:NK Cell Lectin-Like Receptor Subfamily A / Q000235:genetics / Q000378:metabolism*; D047368:Tumor Burden; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics / Q000378:metabolism
chemical_list: D025601:Adenomatous Polyposis Coli Protein; C527093:Klra5 protein, mouse; D055650:NK Cell Lectin-Like Receptor Subfamily A; C000613192:adenomatous polyposis coli protein, mouse; D014568:Urokinase-Type Plasminogen Activator; D001397:Azoxymethane
keywords: Intestinal cancer; Intraepithelial lymphocyte; Ly49E; Urokinase plasminogen activator