title: Fabrication and Application of Isotopically Labelled Gold Arrays for Multiplexed Peptide Analysis.
abstract: A new array-based technology for the simultaneous capture, chemical labelling and mass spectrometry analysis of peptides is presented. Isotopically labelled self-assembled monolayer (SAM) gold arrays are constructed and used simultaneously to capture and label a range of peptides. The array-immobilised, labelled peptides were released by MALDI ablation, analysed by MALDI mass spectrometry and readily identified as labelled peptides from their characteristic isotope pattern. This new solid-phase array platform has the advantage of minimal sample manipulation and is suitable for multiple analyses of single protein digests on a single MALDI target plate.
authors: Manchester Institute of Biotechnology and School of Chemistry, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.
authors: Roberto
authors: R
authors: Castangia
authors: Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK.
authors: Siân R
authors: SR
authors: Hudson
authors: Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK.
authors: Helen K
authors: HK
authors: Robinson
authors: Manchester Institute of Biotechnology and School of Chemistry, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK. Sabine.Flitsch@manchester.ac.uk.
authors: Sabine L
authors: SL
authors: Flitsch
authors: Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. anne.routledge@york.ac.uk.|Centre of Excellence in Mass Spectrometry, University of York, Heslington, York, YO10 5DD, UK. anne.routledge@york.ac.uk.
authors: Jane
authors: J
authors: Thomas-Oates
authors: Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. jane.thomas-oates@york.ac.uk.
authors: Anne
authors: 0000-0003-4008-7545
authors: A
authors: Routledge
mesh_terms: D006046:Gold / Q000737:chemistry*; D007553:Isotope Labeling; D013058:Mass Spectrometry; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis*; D040081:Protein Array Analysis
chemical_list: D010455:Peptides; D006046:Gold
keywords: MALDI mass spectrometry; gold; isotopic labeling; peptides; self-assembled monolayers