title: Delivery of RNA-based molecules to human hematopoietic stem and progenitor cells for modulation of gene expression.
abstract: Gene modulation of human hematopoietic stem and progenitor cells (HSPCs) harbors great potential for therapeutic application of these cells and presents a versatile tool in basic research to enhance our understanding of HSPC biology. However, stable genetic modification might be adverse, particularly in clinical settings. Here, we review a broad range of approaches to transient, nonviral modulation of protein expression with a focus on RNA-based methods. We compare different delivery methods and describe the usefulness of RNA molecules for overexpression as well as downregulation of proteins in HSPCs.
authors: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
authors: Yvonne
authors: Y
authors: Diener
authors: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
authors: Andreas
authors: A
authors: Bosio
authors: Miltenyi Biotec GmbH, Bergisch Gladbach, Germany. Electronic address: ute.bissels@miltenyibiotec.de.
authors: Ute
authors: U
authors: Bissels
mesh_terms: D060846:Cell Engineering; D015870:Gene Expression; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics; D012313:RNA / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics*; D016372:RNA, Antisense / Q000737:chemistry; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D014162:Transfection / Q000379:methods
chemical_list: D035683:MicroRNAs; D016372:RNA, Antisense; D012333:RNA, Messenger; D012313:RNA