title: Wound healing applications of sericin/chitosan-capped silver nanoparticles incorporated hydrogel.
abstract: Microbial contamination in wounds leading to severe sepsis can be treated by silver-based antiseptics. However, frequent application of silver-based antiseptics, staining of skin, burning, and irritation at application site resulted to poor patient compliances. Thus, we formulated sericin- and chitosan-capped silver nanoparticle (S/C-SNP)-loaded hydrogel for accelerated wound healing and antimicrobial properties. The wound healing property of sericin, antibacterial nature of chitosan and silver, and mucoadhesive property of carbopol were utilized in development of novel wound dressing hydrogel to investigate the combined effect of these materials for effective treatment of wounds. The chemical reduction method was successfully employed for the synthesis of SNPs using sericin and chitosan as a capping/reducing agent. The SNPs were characterized by ultraviolet-spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and transmission electron microscopy (TEM). The optimized SNPs were further used for preparation of carbopol hydrogel (0.5, 0.75, and 1.0 % w/v). The prepared hydrogels were characterized for pH, viscosity, and texture analysis. The antimicrobial activity and wound healing activity of the optimized hydrogel (S/C-SNPs G-1) demonstrated higher bactericidal activity and wound closure, as supported by results of histopathology. Hydrogel containing capped SNPs has application in wound healing treatment.
authors: Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, UP, 226025, India.
authors: Jyoti
authors: J
authors: Verma
authors: Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, UP, 226025, India.
authors: Jovita
authors: J
authors: Kanoujia
authors: Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, UP, 226025, India.
authors: Poonam
authors: P
authors: Parashar
authors: Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, UP, 226025, India.
authors: Chandra Bhusan
authors: CB
authors: Tripathi
authors: Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, UP, 226025, India. shubhini.saraf@gmail.com.
authors: Shubhini A
authors: SA
authors: Saraf
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005260:Female; D020100:Hydrogels / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D006863:Hydrogen-Ion Concentration; D008297:Male; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D017208:Rats, Wistar; D047030:Sericins / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D012834:Silver / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D012867:Skin / Q000187:drug effects / Q000473:pathology; D023422:Skin Irritancy Tests; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D014783:Viscosity; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D047030:Sericins; D012834:Silver; D048271:Chitosan
keywords: Capped silver nanoparticles; Chitosan; Hydrogel; Sericin; Wound healing