title: Natural Killer Receptor 1 Dampens the Development of Allergic Eosinophilic Airway Inflammation.
abstract: The function of NCR1 was studied in a model of experimental asthma, classified as a type 1 hypersensitivity reaction, in mice. IgE levels were significantly increased in the serum of OVA immunized NCR1 deficient (NCR1gfp/gfp) mice in comparison to OVA immunized wild type (NCR1+/+) and adjuvant immunized mice. Histological analysis of OVA immunized NCR1gfp/gfp mice revealed no preservation of the lung structure and overwhelming peribronchial and perivascular granulocytes together with mononuclear cells infiltration. OVA immunized NCR+/+ mice demonstrated preserved lung structure and peribronchial and perivascular immune cell infiltration to a lower extent than that in NCR1gfp/gfp mice. Adjuvant immunized mice demonstrated lung structure preservation and no immune cell infiltration. OVA immunization caused an increase in PAS production independently of NCR1 presence. Bronchoalveolar lavage (BAL) revealed NCR1 dependent decreased percentages of eosinophils and increased percentages of lymphocytes and macrophages following OVA immunization. In the OVA immunized NCR1gfp/gfp mice the protein levels of eosinophils' (CCL24) and Th2 CD4+ T-cells' chemoattractants (CCL17, and CCL24) in the BAL are increased in comparison with OVA immunized NCR+/+ mice. In the presence of NCR1, OVA immunization caused an increase in NK cells numbers and decreased NCR1 ligand expression on CD11c+GR1+ cells and decreased NCR1 mRNA expression in the BAL. OVA immunization resulted in significantly increased IL-13, IL-4 and CCL17 mRNA expression in NCR1+/+ and NCR1gfp/gfp mice. IL-17 and TNFα expression increased only in OVA-immunized NCR1+/+mice. IL-6 mRNA increased only in OVA immunized NCR1gfp/gfp mice. Collectively, it is demonstrated that NCR1 dampens allergic eosinophilic airway inflammation.
authors: The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors: Shirin
authors: S
authors: Elhaik Goldman
authors: Department of Clinical Microbiology and Immunology, Sackler school of medicine, Tel-Aviv University, Tel Aviv, Israel.
authors: Itay
authors: I
authors: Moshkovits
authors: The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors: Avishai
authors: A
authors: Shemesh
authors: The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors: Ayelet
authors: A
authors: Filiba
authors: The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors: Yevgeny
authors: Y
authors: Tsirulsky
authors: The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors: Elena
authors: E
authors: Vronov
authors: The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors: Marilou
authors: M
authors: Shagan
authors: The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors: Ron N
authors: RN
authors: Apte
authors: Soroka University Medical Center, Department of Pathology, Bear Sheva, Israel.
authors: D Aniel
authors: DA
authors: Benharroch
authors: Department of Clinical Microbiology and Immunology, Sackler school of medicine, Tel-Aviv University, Tel Aviv, Israel.
authors: Danielle
authors: D
authors: Karo-Atar
authors: Faculty of Health Science, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors: Ron
authors: R
authors: Dagan
authors: Department of Clinical Microbiology and Immunology, Sackler school of medicine, Tel-Aviv University, Tel Aviv, Israel.
authors: Ariel
authors: A
authors: Munitz
authors: The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.|Department of Clinical Microbiology and Immunology, Sackler school of medicine, Tel-Aviv University, Tel Aviv, Israel.
authors: Yaffa
authors: Y
authors: Mizrachi Nebenzahl
authors: The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.|National Institute of Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer Sheva, Israel.
authors: Angel
authors: A
authors: Porgador
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000235:genetics / Q000276:immunology*; D001249:Asthma / Q000139:chemically induced / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D008168:Lung / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D055627:Natural Cytotoxicity Triggering Receptor 1 / Q000235:genetics / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology* / Q000473:pathology
chemical_list: D000950:Antigens, Ly; D016207:Cytokines; D055627:Natural Cytotoxicity Triggering Receptor 1; C118384:Ncr1 protein, mouse