title: Gga-miR-21 inhibits chicken pre-adipocyte proliferation in part by down-regulating Kruppel-like factor 5.
abstract: Gga-miR-21 is abundantly expressed in chicken pre-adipocytes, but its role is unclear. The present study investigated the role of gga-miR-21 in chicken pre-adipocyte proliferation. Cell proliferation assay and gene expression analysis of proliferating cell nuclear antigen (PCNA) showed that the gga-miR-21 mimic inhibited pre-adipocyte proliferation. In contrast, the gga-miR-21 inhibitor enhanced pre-adipocyte proliferation. The subsequent investigation identified Kruppel-like factor 5 (KLF5) mRNA as a target of gga-miR-21. The gga-miR-21 mimic inhibited KLF5 3'UTR reporter activity and decreased endogenous KLF5 expression in primary pre-adipocytes. KLF5 knockdown using RNAi had a similar effect to that of the gga-miR-21 mimic on cell proliferation. The promoting effect of the gga-miR-21 inhibitor on pre-adipocyte proliferation was partially attenuated by KLF5 knockdown. Taken together, our results demonstrated that miR-21 inhibits chicken pre-adipocyte proliferation, at least in part, by targeting KLF5.
authors: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors: Weishi
authors: W
authors: Wang
authors: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors: Min
authors: M
authors: Cheng
authors: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors: Shupei
authors: S
authors: Qiao
authors: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors: Yuxiang
authors: Y
authors: Wang
authors: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China lihui@neau.edu.cn wangning@neau.edu.cn.
authors: Hui
authors: H
authors: Li
authors: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China lihui@neau.edu.cn wangning@neau.edu.cn.
authors: Ning
authors: N
authors: Wang
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D049109:Cell Proliferation; D002645:Chickens / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
chemical_list: D030161:Avian Proteins; D051741:Kruppel-Like Transcription Factors; D035683:MicroRNAs
keywords: KLF5; gga-miR-21; pre-adipocyte; proliferation