title: Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord.
abstract: The adult mammalian forebrain comprises two distinct populations of neural stem cells (NSCs): rare, Oct4 positive, primitive NSCs (pNSCs) and definitive NSC (dNSC) which are more abundant and express GFAP. The pNSCs are upstream of the dNSCs in the neural stem cell lineage. Herein we show that pNSC and dNSC populations can also be isolated from the developing and adult spinal cord. Spinal cord derived pNSCs are similarly rare, Oct4 expressing cells that are responsive to leukemia inhibitory factor and dNSCs are 4-5X more abundant and express GFAP. We demonstrate that myelin basic protein (MBP) is inhibitory to both pNSC and dNSC derived colony formation. Similar to what is seen in the adult forebrain following injury, spinal cord injury results in a significant increase in the size of the dNSC and pNSC pools. Hence, both primitive and definitive neural stem cells can be isolated from along the embryonic and adult neuraxis in vivo and their behavior is regulated by MBP and injury. Stem Cells 2017;35:485-496.
authors: Department of Surgery, University of Toronto, Toronto, Canada.|Institute of Medical Sciences, University of Toronto, Toronto, Canada.
authors: Wenjun
authors: W
authors: Xu
authors: Department of Surgery, University of Toronto, Toronto, Canada.|Institute of Medical Sciences, University of Toronto, Toronto, Canada.
authors: Nadia
authors: N
authors: Sachewsky
authors: Department of Surgery, University of Toronto, Toronto, Canada.|Institute of Medical Sciences, University of Toronto, Toronto, Canada.
authors: Ashkan
authors: A
authors: Azimi
authors: Department of Surgery, University of Toronto, Toronto, Canada.
authors: Maurita
authors: M
authors: Hung
authors: Department of Surgery, University of Toronto, Toronto, Canada.
authors: Andrew
authors: A
authors: Gappasov
authors: Department of Surgery, University of Toronto, Toronto, Canada.|Institute of Medical Sciences, University of Toronto, Toronto, Canada.|Institute of Biomaterial and Biomedical Engineering, University of Toronto, Toronto, Canada.
authors: Cindi M
authors: CM
authors: Morshead
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D049109:Cell Proliferation; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D051858:Forkhead Transcription Factors / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D004676:Myelin Basic Protein / Q000378:metabolism*; D009186:Myelin Sheath / Q000378:metabolism; D064231:Nestin / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D018874:Spheroids, Cellular / Q000166:cytology; D013116:Spinal Cord / Q000166:cytology*; D013119:Spinal Cord Injuries / Q000473:pathology
chemical_list: C481963:FOXJ1 protein, mouse; D051858:Forkhead Transcription Factors; D005904:Glial Fibrillary Acidic Protein; D004676:Myelin Basic Protein; D064231:Nestin; D050814:Octamer Transcription Factor-3
keywords: Adult neural stem cells; GFAP+ neural stem cells; Myelin basic protein; Primitive neural stem cells; Spinal cord injury