title: Detection and comparison of Selenomonas sputigena in subgingival biofilms in chronic and aggressive periodontitis patients.
abstract: BACKGROUND
  With the advent of DNA-based culture-independent techniques, a constantly growing number of Selenomonas phylotypes have been detected in patients with destructive periodontal diseases. However, the prevalence levels that have been determined in different studies vary considerably.
  
  
  OBJECTIVE
  The present study was undertaken to detect and compare the presence of Selenomonas sputigena in the subgingival plaque samples from generalized aggressive periodontitis (GAP), chronic generalized periodontitis, and periodontally healthy patients using conventional polymerase chain reaction (PCR) technique.
  
  
  METHODS
  A total of 90 patients were categorized as periodontally healthy individuals (Group I, n = 30), chronic generalized periodontitis (Group II, n = 30), and GAP (Group III, n = 30). The clinical parameters were recorded and subgingival plaque samples were collected. These were then subjected to conventional PCR analysis.
  
  
  METHODS
  Kruskal-Wallis ANOVA test was used for multiple group comparisons followed by Mann-Whitney U-test for pairwise comparison.
  
  
  RESULTS
  On comparison between three groups, all the clinical parameters were found to be statistically highly significant. Comparing Groups I-II and I-III, the difference in detection was found to be statistically highly significant whereas in Groups II-III, it was statistically nonsignificant. On comparison of S. sputigena detected and undetected patients to clinical parameters in various study groups, the difference was found to be nonsignificant.
  
  
  CONCLUSIONS
  S. sputigena was found to be significantly associated with chronic and aggressive periodontitis. Although the difference in its detection frequency in both groups was statistically nonsignificant when compared clinically, S. sputigena was more closely associated with the GAP.
authors: Department of Periodontics, College of Dental Sciences, Davangere, Karnataka, India.
authors: Disha
authors: D
authors: Nagpal
authors: Department of Periodontics, College of Dental Sciences, Davangere, Karnataka, India.
authors: Shobha
authors: S
authors: Prakash
authors: Department of Microbiology, Karnataka, India; Department of Molecular Biology and Immunology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India.
authors: Kishore Gajanan
authors: KG
authors: Bhat
authors: Department of Prosthodontics, College of Dental Sciences, Davangere, Karnataka, India.
authors: Gagandeep
authors: G
authors: Singh
keywords: Chronic periodontitis; Selenomonas sputigena; generalized aggressive periodontitis; polymerase chain reaction