title: Site-Specific Labeling of MicroRNA Precursors: A Structure-Activity Relationship Study.
abstract: Functionalized oligoribonucleotides are essential tools in RNA chemical biology. Various synthetic routes have been developed over recent years to conjugate functional groups to oligoribonucleotides. However, the presence of the functional group on the oligoribonucleotide backbone can lead to partial or total loss of biological function. The limited knowledge concerning the positioning of functional groups therefore represents a hurdle for the development of oligoribonucleotide chemical tools. Here we describe a systematic investigation of site-specific labeling of pre-miRNAs to identify positions for the incorporation of functional groups, in order not to hinder their processing into active mature miRNAs.
authors: Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, 8093, Zürich, Switzerland.
authors: Mirjam
authors: M
authors: Menzi
authors: Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, 8093, Zürich, Switzerland.
authors: Ugo
authors: U
authors: Pradère
authors: Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, 8093, Zürich, Switzerland.
authors: Yuluan
authors: Y
authors: Wang
authors: Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, 8093, Zürich, Switzerland.
authors: Matteo
authors: M
authors: Fischer
authors: Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, 8093, Zürich, Switzerland.
authors: Fabienne
authors: F
authors: Baumann
authors: Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, 8093, Zürich, Switzerland.
authors: Martina
authors: M
authors: Bigatti
authors: Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, 8093, Zürich, Switzerland. jonathan.hall@pharma.ethz.ch.
authors: Jonathan
authors: J
authors: Hall
mesh_terms: D035683:MicroRNAs / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D035683:MicroRNAs; D009843:Oligoribonucleotides
keywords: RNA recognition; functional groups; gene expression; inhibitors; nucleobases