title: Biophysical Effects of a Polymeric Biosurfactant in Candida krusei and Candida albicans Cells.
abstract: This study evaluated the effects of a polymeric biosurfactant produced by Trichosporon montevideense CLOA72 in the adhesion of Candida albicans and Candida krusei cells to human buccal epithelial cells and its interference in biofilm formation by these strains. The biofilm inhibition by biosurfactant (25 mg/mL) in C. krusei and C. albicans in polystyrene was reduced up to 79.5 and 85 %, respectively. In addition, the zeta potential and hydrodynamic diameter of the yeasts altered as a function of the biosurfactant concentration added to the cell suspension. The changes in the cell surface characteristics and the interface modification can contribute to the inhibition of the initial adherence of yeasts cells to the surface. In addition, the analyses of the biofilm matrix and planktonic cell surfaces demonstrated differences in carbohydrate and protein concentrations for the two studied strains, which may contribute to the modulation of cell adhesion or consolidation of biofilms, especially in C. krusei. This study suggests a possible application of the of CLOA72 biosurfactant in inhibiting the adhesion and formation of biofilms on biological surfaces by yeasts of the Candida genus.
authors: Departamento de Farmácia, Universidade Federal de Juiz de Fora - Campus Governador Valadares, Av. Dr. Raimundo Monteiro de Rezende, 330, Centro, Governador Valadares, MG, 35010-177, Brazil. gabriella.freitas@ufjf.edu.br.
authors: Gabriella Freitas
authors: http://orcid.org/0000-0001-6842-1934
authors: GF
authors: Ferreira
authors: Laboratório de Biologia Parasitária, Universidade CEUMA, Rua Josué Montello, 1, Renascença II, São Luís, MA, 65075-120, Brazil.
authors: Bruna Lorrana
authors: BL
authors: Dos Santos Pinto
authors: Laboratório de Biologia Parasitária, Universidade CEUMA, Rua Josué Montello, 1, Renascença II, São Luís, MA, 65075-120, Brazil.
authors: Eliene Batista
authors: EB
authors: Souza
authors: Laboratório de Biologia Parasitária, Universidade CEUMA, Rua Josué Montello, 1, Renascença II, São Luís, MA, 65075-120, Brazil.
authors: José Lima
authors: JL
authors: Viana
authors: Laboratório de Biologia Parasitária, Universidade CEUMA, Rua Josué Montello, 1, Renascença II, São Luís, MA, 65075-120, Brazil.
authors: Adrielle
authors: A
authors: Zagmignan
authors: Laboratório de Biologia Parasitária, Universidade CEUMA, Rua Josué Montello, 1, Renascença II, São Luís, MA, 65075-120, Brazil.
authors: Julliana Ribeiro Alves
authors: JR
authors: Dos Santos
authors: Universidade Vale do Rio Doce, Rua Israel Pinheiro, 2000, Universitário, Governador Valadares, MG, 35020-220, Brazil.
authors: Áquila Rodrigues Costa
authors: ÁR
authors: Santos
authors: Universidade Vale do Rio Doce, Rua Israel Pinheiro, 2000, Universitário, Governador Valadares, MG, 35020-220, Brazil.
authors: Priscila Batista
authors: PB
authors: Tavares
authors: Departamento de Farmácia, Universidade Federal de Juiz de Fora - Campus Governador Valadares, Av. Dr. Raimundo Monteiro de Rezende, 330, Centro, Governador Valadares, MG, 35010-177, Brazil.
authors: Ângelo Márcio Leite
authors: ÂM
authors: Denadai
authors: Laboratório de Biologia Parasitária, Universidade CEUMA, Rua Josué Montello, 1, Renascença II, São Luís, MA, 65075-120, Brazil.
authors: Andrea Souza
authors: AS
authors: Monteiro
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D055592:Biophysical Phenomena / Q000187:drug effects*; D001704:Biopolymers / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000382:microbiology; D064368:Healthy Volunteers; D006801:Humans; D013501:Surface-Active Agents / Q000302:isolation & purification / Q000494:pharmacology*; D014250:Trichosporon / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D001704:Biopolymers; D013501:Surface-Active Agents
keywords: Biofilm; Biosurfactant; Candida albicans; Candida krusei