title: Biochemical and biological characterization of a dermonecrotic metalloproteinase isolated from Cerastes cerastes snake venom.
abstract: A dermonecrotic metalloproteinase (CcD-II) was isolated from C. cerastes venom. Venom fractionation was performed using three chromatographic steps (molecular exclusion on Sephadex G-75, ion-exchange on DEAE-Sephadex A-50, and reversed-phase high-performance liquid chromatography on C8 column). CcD-II presented an apparent molecular mass of 39.9 kDa and displayed a dermonecrotic activity with a minimal necrotic dose of 0.2 mg/kg body weight. CcD-II showed proteolytic ability on casein chains and on α and β fibrinogen chains that was inhibited by ethylenediamine tetraacetic acid and 1,10-phenanthroline while remained unaffected by phenylmethylsulphonyl fluoride and heparin. CcD-II displayed gelatinase activity and degraded extracellular matrix compounds (type-IV collagen and laminin). These results correlated with histopathological analysis showing a complete disorganization of collagenous skin fibers. These data suggested that CcD-II belongs to P-II class of snake venom metalloproteinase. The characterization of venom compounds involved in tissue damage may contribute in the development of new therapeutic strategies in envenomation.
authors: USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, Bab Ezzouar, Algiers, Algeria.
authors: Amina
authors: A
authors: Ami
authors: USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, Bab Ezzouar, Algiers, Algeria.
authors: Habiba
authors: H
authors: Oussedik-Oumehdi
authors: USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, Bab Ezzouar, Algiers, Algeria.
authors: Fatima
authors: F
authors: Laraba-Djebari
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism; D045726:Metalloproteases / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009336:Necrosis; D059748:Proteolysis; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D014757:Viper Venoms / Q000201:enzymology* / Q000633:toxicity; D017819:Viperidae
chemical_list: D016326:Extracellular Matrix Proteins; D014757:Viper Venoms; D005780:Gelatin; D005340:Fibrinogen; D045726:Metalloproteases
keywords: Dermonecrosis; extracellular matrix; metalloproteinase; viper venom