title: [Exogenous hydrogen sulfide inhibits high-glucose-induced injuries via regulating leptin/leptin receptor signaling pathway in human umbilical vein endothelial cells].
abstract: OBJECTIVE
  To investigate whether exogenous hydrogen sulfide (H2S) inhibits the high-glucose (HG)-induced injury by modulating leptin/leptin receptor (LEPR) signal pathway in human umbilical vein endothelial cells (HUVECs).
  
  
  METHODS
  HUVECs were treated with 40 mmol/L glucose for 3-24 h, and the cell viability was examined by CCK-8 assay. The changes of cell morphology and the number of apoptotic cells were assessed by Hoechst 33258 nuclear staining followed by photofluorography. The intracellular levels of reactive oxygen species (ROS) was detected by DCFH-DA staining followed by photofluorography. Mitochondrial membrane potential (MMP) was determined by Rhodamine 123 (Rh123) staining and photofluorography. The expression levels of leptin and LEPR protein were measured by Western blotting.
  
  
  RESULTS
  s The expression of leptin and LERP in HUVECs began to significantly increase at 3 h after HG exposure and reached the peak levels at 9 h (P<0.01). Pretreatment of HUVECs with 400 µmol/L sodium hydrosulfide (H2S donor) for 30 min inhibited HG-induced increase in leptin and leptin receptor expressions in HUVECs (P<0.01). Pretreatment of HUVECs with 400 µmol/L NaHS for 30 min or 50 ng/mL leptin antagonists (LA) for 1 h obviously alleviated HG-induced injury by increasing cell viability, decreasing cell apoptosis and lowering accumulation of intracellular ROS and MMP loss (P<0.01).
  
  
  CONCLUSIONS
  Exogenous H2S protects against HG-induced injury by inhibiting leptin/LEPR pathway in HUVECs.
authors: 1Southern Medical University, Guangzhou 510515, China; 2Department of Endocrinology, East Ward, Guangdong Academy of Medical Sciences/Guangdong General Hospital, Guangzhou 510080, China; 3Department of Endocrinology, Liuzhou Hospital Affiliated to Southern Medical University, Liuzhou 545007, China. E-mail: alym41@sina.com.
authors: Dong-Bo
authors: DB
authors: Wu
authors: Jing-Fu
authors: JF
authors: Chen
authors: Qing
authors: Q
authors: Xu
authors: Jia-Qiong
authors: JQ
authors: Lin
authors: Jing-Qiu
authors: JQ
authors: Liao
authors: Wen
authors: W
authors: Wu
mesh_terms: D017209:Apoptosis; D002470:Cell Survival; D002478:Cells, Cultured; D005947:Glucose / Q000009:adverse effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D020738:Leptin / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial; D017382:Reactive Oxygen Species / Q000378:metabolism; D054411:Receptors, Leptin / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D020738:Leptin; D017382:Reactive Oxygen Species; D054411:Receptors, Leptin; D005947:Glucose; D006862:Hydrogen Sulfide