title: Mitochondrial haplogroups and expression studies of commonly used human cell lines.
abstract: We developed a multiplex fragment length analysis (MFLA) for clearly assigning mitochondrial haplogroups mostly endemic in Europe for future cardiac diagnostics. As a technical proof, 23 commonly used human cell lines were haplotyped as reference standards. The functional analysis on mtDNA copies per cell revealed no correlation to haplogroups but a relatively high rate of mitochondria per cell and at the same time a very low expression of all mitochondrial and some nuclear encoded mitochondrial related genes. Established MFLA is an easy to handle method for analysing European mitochondrial haplogroups to perform epidemic studies and elucidate correlations to distinct diseases.
authors: Institute for Cardiac Diagnostics and Therapy (IKDT), Moltkestr. 31, D-12203 Berlin, Germany. Electronic address: Christine.Siegismund@ikdt.de.
authors: Christine Sabine
authors: CS
authors: Siegismund
authors: University of Leipzig, Centre for Biotechnology and Biomedicine (BBZ), Molecular Cell Therapy, Deutscher Platz 5, D-04103 Leipzig, Germany.
authors: Ingo
authors: I
authors: Schäfer
authors: University of Leipzig, Centre for Biotechnology and Biomedicine (BBZ), Molecular Cell Therapy, Deutscher Platz 5, D-04103 Leipzig, Germany.
authors: Peter
authors: P
authors: Seibel
authors: Institute for Cardiac Diagnostics and Therapy (IKDT), Moltkestr. 31, D-12203 Berlin, Germany; Department of Cardiology, Campus Virchow, Charité - University Hospital Berlin, Augustenburger Platz 1, D-13353, Germany.
authors: Uwe
authors: U
authors: Kühl
authors: Institute for Cardiac Diagnostics and Therapy (IKDT), Moltkestr. 31, D-12203 Berlin, Germany.
authors: Heinz-Peter
authors: HP
authors: Schultheiss
authors: Institute for Cardiac Diagnostics and Therapy (IKDT), Moltkestr. 31, D-12203 Berlin, Germany.
authors: Dirk
authors: D
authors: Lassner
mesh_terms: D002460:Cell Line; D016172:DNA Fingerprinting; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000592:standards; D005060:Europe; D050259:Genes, Mitochondrial; D006239:Haplotypes; D006801:Humans; D008928:Mitochondria / Q000235:genetics*; D058889:Molecular Typing
chemical_list: D004272:DNA, Mitochondrial
keywords: Cell lines; Gene expression analysis; Mitochondria; Mitochondrial haplogroup; Multiplex fragment length analysis; mtDNA