title: Novel Polymorphic Microsatellite Markers for Panulirus ornatus and their Cross-species Primer Amplification in Panulirus homarus.
abstract: Polymorphic microsatellite loci were isolated for Panulirus ornatus using 454 GS-FLX Titanium pyrosequencing. Fifteen markers containing perfect di-, tri-, tetra-, and penta-nucleotide motifs were consistently co-amplified in five multiplexes in a panel of 91 randomly selected samples. Observed number of alleles varied from 2 to 14 per locus. Observed and expected heterozygosity ranged from 0.090 to 0.79 and 0.08 to 0.87, respectively. Ten loci deviated from Hardy-Weinberg equilibrium after sequential Bonferroni correction. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between 11 loci. The microsatellite markers were also amplified successfully in related Panulirus homarus species with adequate level of polymorphism. The successful cross-species primer amplification of the 15 microsatellites indicates the potential of the developed markers to be transferred to other Panulirus species. The 15 novel microsatellite markers reported in this work add to the previously characterized markers by our group, exhibit adequate levels of polymorphism for wide range of future studies investigating population structure, genetic diversity, and evolutionary relationships among Panulirus species.
authors: a Center of Excellence in Marine Biotechnology , Sultan Qaboos University , Al-Khoud , Sultanate of Oman.
authors: Madjid
authors: M
authors: Delghandi
authors: a Center of Excellence in Marine Biotechnology , Sultan Qaboos University , Al-Khoud , Sultanate of Oman.
authors: Hasifa
authors: H
authors: Afzal
authors: a Center of Excellence in Marine Biotechnology , Sultan Qaboos University , Al-Khoud , Sultanate of Oman.
authors: Manal Saif Nasser
authors: MS
authors: Al Hinai
authors: a Center of Excellence in Marine Biotechnology , Sultan Qaboos University , Al-Khoud , Sultanate of Oman.
authors: Rafaida Dhuhai Gharib
authors: RD
authors: Al-Breiki
authors: b Center for Sustainable Tropical Fisheries and Aquaculture and College of Marine and Environmental Science , James Cook University , Townsville , Queensland , Australia.
authors: Dean R
authors: DR
authors: Jerry
authors: b Center for Sustainable Tropical Fisheries and Aquaculture and College of Marine and Environmental Science , James Cook University , Townsville , Queensland , Australia.|c Institute of Oceanography , Vietnam Academy of Science and Technology , Nha Trang , Vietnam.
authors: Hoc Tan
authors: HT
authors: Dao
mesh_terms: D000818:Animals; D005819:Genetic Markers / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D033521:Palinuridae / Q000235:genetics*; D013045:Species Specificity
chemical_list: D005819:Genetic Markers
keywords: 454 sequencing; microsatellites; multiplexing; panulirus; spiny lobster