title: Simultaneous Determination of 25 Ergot Alkaloids in Cereal Samples by Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry.
abstract: A liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of 25 ergot alkaloids in cereal samples. The analytes included both -ine and -inine ergot alkaloids and were extracted using an acetonitrile and ammonium carbonate solution, followed by purification with C-18 sorbent. After full separation on a C18 column, the 25 ergot alkaloids were detected by LC-MS/MS using multiple reaction monitoring (MRM) in the positive ion mode. The linear range was 0.05-5.0 μg/kg for the 25 ergot alkaloids. The mean recoveries at three spiked concentrations varied from 76.5 to 120% with RSD < 15%. This method was validated using a FAPAS proficiency test sample of ergot alkaloids in rye flour and was finally applied to analyze real samples, including rye flours, wheat flours, whole wheat flours, bread, and noodles.
authors: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control & Prevention , Beijing 100013, China.|College of Science, Beijing University of Chemical Technology , Beijing 100029, China.
authors: Qiaozhen
authors: Q
authors: Guo
authors: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control & Prevention , Beijing 100013, China.
authors: Bing
authors: B
authors: Shao
authors: College of Science, Beijing University of Chemical Technology , Beijing 100029, China.
authors: Zhenxia
authors: Z
authors: Du
authors: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control & Prevention , Beijing 100013, China.
authors: Jing
authors: J
authors: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D004876:Ergot Alkaloids / Q000737:chemistry* / Q000302:isolation & purification; D005433:Flour / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D012434:Secale / Q000737:chemistry; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014908:Triticum / Q000737:chemistry
chemical_list: D004876:Ergot Alkaloids; D010936:Plant Extracts
keywords: cereal sample; ergot alkaloids; ultraperformance liquid chromatography−tandem mass spectrometry