title: Comparative studies of adhesion peptides based on l- or d-amino acids.
abstract: Detailed studies comparing solid-supported l- or d-amino acid adhesion peptides based on the sequence KLHRIRA were performed. Stability towards proteases and levels of cellular adhesion to the otherwise inert surface of PEGA resin were compared by using fluorescently labelled peptides. A clear difference in the peptide stability towards cleavage by subtilisin, trypsin, or papain was observed. However, all of the on-bead peptides provided an optimal surface for cell adhesion and proliferation. In long-term experiments, these properties were still found to be similar on the resins modified either with l- or with d-amino acids and unaffected by the nature of their fluorescence labelling at either terminus. These results support that the more accessible l-amino acids can be utilized for cell adhesion experiments and confirm the nonspecific interaction mechanism of cell binding to these peptides on the bead surface. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd.
authors: Center for Evolutionary Chemical Biology, University of Copenhagen, Copenhagen, Denmark.|Novosibirsk State University, 2 Pirogova St., Novosibirsk, 630090, Russia.
authors: Sergey
authors: S
authors: Nikitin
authors: Center for Evolutionary Chemical Biology, University of Copenhagen, Copenhagen, Denmark.
authors: Daniel
authors: D
authors: Palmer
authors: Center for Evolutionary Chemical Biology, University of Copenhagen, Copenhagen, Denmark. meldal@nano.ku.dk.
authors: Morten
authors: M
authors: Meldal
authors: Center for Evolutionary Chemical Biology, University of Copenhagen, Copenhagen, Denmark. fdi@chem.ku.dk.
authors: Frederik
authors: F
authors: Diness
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology; D000269:Adhesives / Q000138:chemical synthesis* / Q000494:pharmacology; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D010206:Papain / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D055550:Protein Stability; D059748:Proteolysis; D060327:Solid-Phase Synthesis Techniques; D013194:Staining and Labeling; D013237:Stereoisomerism; D020860:Subtilisin / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D000269:Adhesives; D000596:Amino Acids; D010455:Peptides; C520358:poly(acryloyl-bis(aminopropyl)polyethylene glycol); D011092:Polyethylene Glycols; D014357:Trypsin; D020860:Subtilisin; D010206:Papain
keywords: PEGA resin; cell adhesion; cells on beads; fluorescence labelling; l-amino acids