title: Identification of long noncoding RNAs as potential novel diagnosis and prognosis biomarkers in colorectal cancer.
abstract: BACKGROUND
  Colorectal cancer (CRC) is prevalent worldwide, and improvements in timely and effective diagnosis are imperatively needed. We aimed to identify potential long noncoding RNA (lncRNA) biomarkers for CRC diagnosis along with prognosis prediction.
  
  
  METHODS
  LncRNA expression profiles were studied by microarray in paired tumor and normal tissues from six patients with CRC. The expression levels of candidate lncRNAs were analyzed in 80 pairs of tissues and two independent cohorts of serum samples. Receiver-operating characteristic (ROC) curves were employed to evaluate the performance of the lncRNAs identified. The correlation between lncRNAs and disease-specific survival rate of CRC patients was assessed to explore prognostic potential.
  
  
  RESULTS
  Four lncRNAs (BANCR, NR_026817, NR_029373, and NR_034119) were identified to be significantly dysregulated in both tissue and serum samples with consistent pattern, and a panel was established based on this result. The performance of the 4-lncRNA panel was measured with an area under the ROC curve (AUC) of 0.881. The corresponding AUCs of the panel for patients with TNM stageI, II and III were 0.774, 0.844 and 0.949, respectively, significantly higher than that of CEA. Kaplan-Meier analysis showed that patients with low levels of NR_029373 and NR_034119 had significantly lower disease-specific survival rate (p = 0.013 and 0.044, respectively). Multivariate Cox analysis demonstrated that NR_029373 and NR_034119 were both independently associated with disease-specific survival rate (p = 0.013 and 0.038, respectively).
  
  
  CONCLUSIONS
  Our study established a distinctive 4-lncRNA panel with considerable diagnostic value and identified NR_029373 and NR_034119 as potential biomarkers for CRC prognosis prediction.
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China.
authors: Rui
authors: R
authors: Wang
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China.
authors: Lutao
authors: L
authors: Du
authors: Department of Gastroenterology, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China.
authors: Xiaoyun
authors: X
authors: Yang
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China.
authors: Xiumei
authors: X
authors: Jiang
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China.
authors: Weili
authors: W
authors: Duan
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China.
authors: Suzhen
authors: S
authors: Yan
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China.
authors: Yujiao
authors: Y
authors: Xie
authors: Department of Clinical Laboratory, Jinxiang County People's Hospital, Jining, 272000, Shandong Province, China.
authors: Yuntao
authors: Y
authors: Zhu
authors: Department of Medical Affairs Management, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China.
authors: Qingliang
authors: Q
authors: Wang
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China.
authors: Lili
authors: L
authors: Wang
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China.
authors: Yongmei
authors: Y
authors: Yang
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China. cxwang@sdu.edu.cn.
authors: Chuanxin
authors: C
authors: Wang
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D006801:Humans; D046228:Microarray Analysis / Q000379:methods; D011237:Predictive Value of Tests; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding
keywords: Colorectal cancer; Diagnosis; LncRNA; Noninvasive biomarkers; Prognosis