title: Controlling Growth and Osteogenic Differentiation of Osteoblasts on Microgrooved Polystyrene Surfaces.
abstract: Surface topography is increasingly being recognized as an important factor to control the response of cells and tissues to biomaterials. In the current study, the aim was to obtain deeper understanding of the effect of microgrooves on shape and orientation of osteoblast-like cells and to relate this effect to their proliferation and osteogenic differentiation. To this end, two microgrooved polystyrene (PS) substrates, differing in the width of the grooves (about 2 μm and 4 μm) and distance between individual grooves (about 6 μm and 11 μm, respectively) were fabricated using a combination of photolithography and hot embossing. MG-63 human osteosarcoma cells were cultured on these microgrooved surfaces, with unpatterned hot-embossed PS substrate as a control. Scanning electron- and fluorescence microscopy analyses showed that on patterned surfaces, the cells aligned along the microgrooves. The cells cultured on 4 μm-grooves / 11 μm-ridges surface showed a more pronounced alignment and a somewhat smaller cell area and cell perimeter as compared to cells cultured on surface with 2 μm-grooves / 6 μm-ridges or unpatterned PS. PrestoBlue analysis and quantification of DNA amounts suggested that microgrooves used in this experiment did not have a strong effect on cell metabolic activity or proliferation. However, cell differentiation towards the osteogenic lineage was significantly enhanced when MG-63 cells were cultured on the 2/6 substrate, as compared to the 4/11 substrate or unpatterned PS. This effect on osteogenic differentiation may be related to differences in cell spreading between the substrates.
authors: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China.|Oral Implantology Center, Stomatology Hospital of Jinan, Jinan, Shandong Province, China.|Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, Overijssel, The Netherlands.
authors: Lanying
authors: L
authors: Sun
authors: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, Overijssel, The Netherlands.|MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, Limburg, The Netherlands.
authors: Daniel
authors: D
authors: Pereira
authors: Oral Implantology Center, Stomatology Hospital of Jinan, Jinan, Shandong Province, China.
authors: Qibao
authors: Q
authors: Wang
authors: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, Overijssel, The Netherlands.|MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, Limburg, The Netherlands.
authors: David Baião
authors: DB
authors: Barata
authors: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, Overijssel, The Netherlands.|MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, Limburg, The Netherlands.
authors: Roman
authors: R
authors: Truckenmüller
authors: Oral Implantology Center, Stomatology Hospital of Jinan, Jinan, Shandong Province, China.
authors: Zhaoyuan
authors: Z
authors: Li
authors: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China.
authors: Xin
authors: X
authors: Xu
authors: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, Overijssel, The Netherlands.|MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, Limburg, The Netherlands.
authors: Pamela
authors: P
authors: Habibovic
mesh_terms: D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D011137:Polystyrenes / Q000009:adverse effects / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D011137:Polystyrenes