title: Tumor necrosis factor receptor-associated factor 6 (TRAF6) participates in anti-lipopolysaccharide factors (ALFs) gene expression in mud crab.
abstract: Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a key cytoplasm signal adaptor that mediates signals activated by tumor necrosis factor receptor (TNFR) superfamily and the Interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily. The full-length 2492 bp TRAF6 (Sp-TRAF6) from Scylla paramamosain contains 1800 bp of open reading frame (ORF) encoding 598 amino acids, including an N-terminal RING-type zinc finger, two TRAF-type zinc fingers and a conserved C-terminal meprin and TRAF homology (MATH) domain. Multiple alignment analysis shows that the putative amino acid sequence of Sp-TRAf6 has highest identity of 88% with Pt-TRAF6 from Portunus trituberculatus, while the similarity of Sp-TRAF6 with other crustacean sequences was 54-55%. RT-PCR analysis indicated that Sp-TRAF6 transcripts were predominantly expressed in the hepatopancreas and stomach, whereas it was barely detected in the heart and hemocytes in our study. Moreover, Sp-TRAF6 transcripts were significantly up-regulated after Vibrio parahemolyticus and LPS challenges. RNA interference assay was carried out used by siRNA to investigate the genes expression patterns regulated by Sp-TRAF6. The qRT-PCR results showed that silencing Sp-TRAF6 gene could inhibit SpALF1, SpALF2, SpALF5 and SpALF6 expression in hemocytes, while inhibit SpALF1, SpALF3, SpALF4, SpALF5 and SpALF6 expression in hepatopancreas. Taken together, the acute-phase response to immune challenges and the inhibition of SpALFs gene expression indicate that Sp-TRAF6 plays an important role in host defense against pathogen invasions via regulation of ALF gene expression in S. paramamosain.
authors: Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China; Marine Biology Institute, Shantou University, Shantou 515063, China.
authors: Wan-Wei
authors: WW
authors: Sun
authors: Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China; Marine Biology Institute, Shantou University, Shantou 515063, China.
authors: Xin-Xu
authors: XX
authors: Zhang
authors: Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China; Marine Biology Institute, Shantou University, Shantou 515063, China.
authors: Wei-Song
authors: WS
authors: Wan
authors: Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China; Marine Biology Institute, Shantou University, Shantou 515063, China.
authors: Shu-Qi
authors: SQ
authors: Wang
authors: Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China.
authors: Xiao-Bo
authors: XB
authors: Wen
authors: Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China; Marine Biology Institute, Shantou University, Shantou 515063, China.
authors: Huai-Ping
authors: HP
authors: Zheng
authors: Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China; Marine Biology Institute, Shantou University, Shantou 515063, China.
authors: Yue-Ling
authors: YL
authors: Zhang
authors: Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China; Marine Biology Institute, Shantou University, Shantou 515063, China. Electronic address: lisk@stu.edu.cn.
authors: Sheng-Kang
authors: SK
authors: Li
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism*; D003386:Brachyura / Q000276:immunology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D043143:Hepatopancreas / Q000378:metabolism*; D007113:Immunity, Innate; D008070:Lipopolysaccharides / Q000276:immunology; D010802:Phylogeny; D034741:RNA, Small Interfering / Q000235:genetics; D016415:Sequence Alignment; D015398:Signal Transduction; D048029:TNF Receptor-Associated Factor 6 / Q000235:genetics / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000378:metabolism; D059467:Transcriptome; D014735:Vibrio Infections / Q000276:immunology*; D014736:Vibrio parahaemolyticus / Q000276:immunology*
chemical_list: D060829:Arthropod Proteins; D008070:Lipopolysaccharides; D034741:RNA, Small Interfering; D048029:TNF Receptor-Associated Factor 6; D051193:Toll-Like Receptors
keywords: Anti-lipopolysaccharide factors; Scylla paramamosain; TRAF6