title: Magnetic solid-phase extraction of protein by ionic liquid-coated Fe@graphene oxide.
abstract: Amino functional dicationic ionic liquid (AFDCIL) was prepared and then coated on the surface of magnetic graphene oxide (GO) as a new magnetic adsorbent (Fe@GO@AFDCIL) for the magnetic solid-phase extraction (MSPE) of protein. The Fe@GO@AFDCIL composite was characterized by vibrating sample magnetometer (VSM), X-ray diffraction (XRD), Fourier transform infrared spectrometry (FT-IR), thermal gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM) and zeta-potential nanoparticles. The bovine hemoglobin (BHb) was used as the analyte, and the extraction performance of Fe@GO@AFDCIL was investigated in the MSPE procedure. The concentration of BHb in samples was determined by a UV-vis spectrophotometer. A comparative investigation of Fe@GO@AFDCIL composite and traditional IL-coated Fe@GO composites (Fe@GO@IL) exhibited the benefits of Fe@GO@AFDCIL. The adsorbed BHb could be eluted from the Fe@GO@AFDCIL by 4% sodium dodecyl sulfate (SDS) solution. The Fe@GO@AFDCIL exhibited favorable stability which could be reused at least 15 times. Under the optimized condition, the real samples were investigated, which demonstrated that the Fe@GO@AFDCIL was able to be applied in extracting bovine hemoglobin (BHb) from real biological samples.
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 PR China.
authors: Qian
authors: Q
authors: Wen
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 PR China. Electronic address: wyzss@hnu.edu.cn.
authors: Yuzhi
authors: Y
authors: Wang
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 PR China.
authors: Kaijia
authors: K
authors: Xu
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 PR China.
authors: Na
authors: N
authors: Li
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 PR China.
authors: Hongmei
authors: H
authors: Zhang
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 PR China.
authors: Qin
authors: Q
authors: Yang
authors: Department of Microbiology, College of Basic Medicine, Central South University, Changsha, 410083 PR China.
authors: Yigang
authors: Y
authors: Zhou
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006108:Graphite / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D060328:Magnetic Phenomena; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D010087:Oxides / Q000737:chemistry*; D052616:Solid Phase Extraction; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine; D013696:Temperature; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D006454:Hemoglobins; D052578:Ionic Liquids; D010087:Oxides; D006108:Graphite; D007501:Iron
keywords: Bovine hemoglobin; Magnetic graphene oxide; Magnetic solid-phase extraction; Mino functional dicationic ionic liquid; Protein