title: Apolipoprotein A1 and heterogeneous nuclear ribonucleoprotein E1 implicated in the regulation of embryo implantation by inhibiting lipid peroxidation.
abstract: It is known that apolipoprotein A1 (apoA1) is a stimulator of endothelial nitric oxide synthase (eNOS), and that heterogeneous nuclear ribonucleoprotein E1 (hnRNP-E1)-containing RNP complexes is a key protector of basal stabilization of eNOS mRNA. Recently, we found that apoA1 and hnRNP-E1 were up-regulated during peri-implantation period, and the purpose of this study was to explore the roles of apoA1 and hnRNP-E1 during this period in the mouse. It was found that the up-regulation of apoA1 and hnRNP-E1 were dependent on the presence and status of blastocysts, on endometrial decidualization and on the progesterone and 17β-oestradiol status. Knockdown of apoA1 or hnRNP-E1 both resulted in reduced numbers of embryo implantations and neonates (P < 0.01), and lipid peroxidation was found to be involved. On pregnancy day 5 eNOS expression and superoxidase dismutase (SOD) quantity were increased, and malondialdehyde (MDA) quantity was decreased at implantation sites. The knockdown of either apoA1 or hnRNP-E1 led to down-regulation of eNOS (P < 0.01) and to an increase in the quantity of MDA (P < 0.05), and a decrease in the amount of SOD (P < 0.01). These findings suggest that apoA1 and hnRNP-E1 may play roles in embryo implantation by inhibiting lipid peroxidation.
authors: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
authors: Jia
authors: J
authors: Jia
authors: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
authors: Jinhai
authors: J
authors: Gou
authors: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
authors: Xia
authors: X
authors: Zhao
authors: Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
authors: Tao
authors: T
authors: Yi
authors: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu 610041, China. Electronic address: zhengyuli@scu.edu.cn.
authors: Zhengyu
authors: Z
authors: Li
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I / Q000235:genetics / Q000502:physiology*; D010064:Embryo Implantation / Q000235:genetics*; D004717:Endometrium / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics / Q000502:physiology*; D015227:Lipid Peroxidation / Q000235:genetics*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016632:Apolipoprotein A-I; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D008315:Malondialdehyde; D052250:Nitric Oxide Synthase Type III; D013482:Superoxide Dismutase
keywords: apolipoprotein A1; embryo implantation; heterogeneous nuclear ribonucleoprotein E1; lipid peroxidation