title: High-Throughput Screening of Tyrosine Kinase Inhibitor Resistant Genes in CML.
abstract: Genome-wide RNA interference (RNAi) screening in mammalian cells has proven to be a powerful tool for identifying new genes and molecular pathways relevant to many cellular processes and diseases. For example, screening for genes that, when inactivated, lead to resistance to cancer therapeutic drugs can reveal new mechanisms for how resistance develops and identify potential targetable strategies to overcome drug resistance. Here, we describe a detailed procedure for performing a high-throughput RNAi screen using a genome-wide human short hairpin RNA (shRNA) library for identifying tyrosine kinase inhibitor (TKI)-resistance genes in a human CML cell line model.
authors: Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA. leyuan.ma@umassmed.edu.|Howard Hughes Medical Institute, Chevy Chase, MD, USA. leyuan.ma@umassmed.edu.
authors: Leyuan
authors: L
authors: Ma
authors: Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA.
authors: Justine
authors: J
authors: Roderick
authors: Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA.
authors: Michelle A
authors: MA
authors: Kelliher
authors: Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA. michael.green@umassmed.edu.|Howard Hughes Medical Institute, Chevy Chase, MD, USA. michael.green@umassmed.edu.
authors: Michael R
authors: MR
authors: Green
mesh_terms: D019008:Drug Resistance, Neoplasm; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D015723:Gene Library; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D000068877:Imatinib Mesylate / Q000494:pharmacology; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy / Q000235:genetics*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D047428:Protein Kinase Inhibitors; D034741:RNA, Small Interfering; D000068877:Imatinib Mesylate; D016044:Fusion Proteins, bcr-abl
keywords: CML; Drug resistance; Genome-wide; Imatinib; RNAi; Tyrosine kinase inhibitor; shRNA screen