title: Comparative transcriptome analysis of unripe and mid-ripe fruit of Mangifera indica (var. "Dashehari") unravels ripening associated genes.
abstract: Ripening in mango is under a complex control of ethylene. In an effort to understand the complex spatio-temporal control of ripening we have made use of a popular N. Indian variety "Dashehari" This variety ripens from the stone inside towards the peel outside and forms jelly in the pulp in ripe fruits. Through a combination of 454 and Illumina sequencing, a transcriptomic analysis of gene expression from unripe and midripe stages have been performed in triplicates. Overall 74,312 unique transcripts with ≥1 FPKM were obtained. The transcripts related to 127 pathways were identified in "Dashehari" mango transcriptome by the KEGG analysis. These pathways ranged from detoxification, ethylene biosynthesis, carbon metabolism and aromatic amino acid degradation. The transcriptome study reveals differences not only in expression of softening associated genes but also those that govern ethylene biosynthesis and other nutritional characteristics. This study could help to develop ripening related markers for selective breeding to reduce the problems of excess jelly formation during softening in the "Dashehari" variety.
authors: CSIR- National Botanical Research Institute, Lucknow, 226001, India.|Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India.
authors: Smriti
authors: S
authors: Srivastava
authors: Umeå Plant Science Centre, Umea, Sweden.
authors: Rajesh K
authors: RK
authors: Singh
authors: CSIR- National Botanical Research Institute, Lucknow, 226001, India.|Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India.
authors: Garima
authors: G
authors: Pathak
authors: CSIR- National Botanical Research Institute, Lucknow, 226001, India.|Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India.
authors: Ridhi
authors: R
authors: Goel
authors: CSIR- National Botanical Research Institute, Lucknow, 226001, India.
authors: Mehar Hasan
authors: MH
authors: Asif
authors: CSIR- National Botanical Research Institute, Lucknow, 226001, India.
authors: Aniruddha P
authors: AP
authors: Sane
authors: CSIR- National Botanical Research Institute, Lucknow, 226001, India.
authors: Vidhu A
authors: VA
authors: Sane
mesh_terms: D002473:Cell Wall / Q000235:genetics; D005030:Ethylenes / Q000096:biosynthesis; D005638:Fruit / Q000235:genetics* / Q000254:growth & development*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D031022:Mangifera / Q000235:genetics* / Q000254:growth & development*; D058977:Molecular Sequence Annotation; D010858:Pigmentation / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000235:genetics
chemical_list: D005030:Ethylenes; C036216:ethylene