title: Comparation of the effects of different 5'-untranslated regions (UTRs) on gene expression in HEK293 cells.
abstract: OBJECTIVE
  To evaluate four 5'-UTRs on GFP expression in HEK293T cells.
  
  
  RESULTS
  The recombinant plasmids were constructed by restriction enzyme digestion, digestion and DNA sequencing. Quantitative real-time PCR and western blotting results showed that the transcription and translation level of PPRV-GFP mRNA was significantly lower than that of the other reporters. The transcription and translation level of ChEF1-GFP was the highest in HEK293T cells.
  
  
  CONCLUSIONS
  Different UTRs can significantly affect protein expression. Additionally, the findings also will be useful in biological applications that require tuning of gene expression and system optimization.
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, People's Republic of China.
authors: Xue-Mei
authors: XM
authors: Zhang
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, People's Republic of China.
authors: Guang-Ming
authors: GM
authors: Zha
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, People's Republic of China.
authors: Jiang
authors: J
authors: Wang
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, People's Republic of China.
authors: Xin-Jian
authors: XJ
authors: Wang
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, People's Republic of China.
authors: Shuang
authors: S
authors: Song
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, People's Republic of China.
authors: Jing-Chao
authors: JC
authors: Shu
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, People's Republic of China.
authors: Bei-Bei
authors: BB
authors: Chu
authors: Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, People's Republic of China. 516689530@qq.com.|College of Animal Husbandary and Veterinary Science, Henan Agricultural University, Wenhua Road 95, Zhengzhou, 450002, People's Republic of China. 516689530@qq.com.
authors: Guo-Yu
authors: GY
authors: Yang
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans
chemical_list: D020121:5' Untranslated Regions; D049452:Green Fluorescent Proteins
keywords: Gene expression; Green fluorescent protein (GFP) expression; Untranslated regions (UTRs); Vectors; Viruses