title: Antinociceptive activity of a polysaccharide from the roots of Sophora flavescens.
abstract: A polysaccharide (SFWP), with a molecular weight of 51kDa, was successfully purified from the roots of Sophora flavescens and the antinociceptive actions of SFWP were evaluated using acetic acid induced writhing, tail flick, and formalin tests in mice. GC-MS results showed that SFWP had a backbone composed of (1→2)-linked Glc, (1→2,6)-inkedGal and (1→3,6)-inked Man residues, which were terminated with (1→)-inked Xyl and (1→)-inked Ara at O-6 of (1→2,6)-inkedGal and (1→3,6)-inked Man along the main chain, in the ratio of 2.0: 1.02: 1.09: 1.10: 0.98. Data showed that SFWP (100, 200 and 400mg/kg) significantly reduced the number of writhings induced by acetic acid in a dose-dependent manner. However, SFWP did not produce analgesia in tail-flick test. Moreover SFWP strongly attenuated the formalin-induced flinching behaviour in the second phases but not in the first phase in a dose-dependent manner. These results revealed that SFWP could be used safely to attenuate both inflammatory and peripheral neuropathic pain.
authors: The Department of Pain Clinic of the First Affiliated Hospital, Harbin Medical University, Harbin, 150001, PR China.
authors: Ruimei
authors: R
authors: Jia
authors: The Department of Pain Clinic of the First Affiliated Hospital, Harbin Medical University, Harbin, 150001, PR China.
authors: Quancheng
authors: Q
authors: Li
authors: The Department of Tumor of the Second Affiliated Hospital, Harbin Medical University, Harbin, 150086, PR China.
authors: Weixi
authors: W
authors: Shen
authors: The Department of Abdominal Ultrasonography Room of the First Affiliated Hospital, Harbin Medical University, Harbin, 150001, PR China.
authors: Jiuwei
authors: J
authors: Zhang
authors: The Anesthesiology Department of the Tumor Hospital Affiliated to Harbin Medical University, Harbin, 150081, PR China.
authors: Lihong
authors: L
authors: Zheng
authors: The Anesthesiology Department of the Tumor Hospital Affiliated to Harbin Medical University, Harbin, 150081, PR China. Electronic address: guonianwang@126.com.
authors: Guonian
authors: G
authors: Wang
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D009437:Neuralgia / Q000188:drug therapy*; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D029909:Sophora / Q000737:chemistry*
chemical_list: D000700:Analgesics; D011134:Polysaccharides
keywords: Antinociceptive activity; Polysaccharide; Sophora flavescens Ait