title: Association between Type A blaZ Gene Polymorphism and Cefazolin Inoculum Effect in Methicillin-Susceptible Staphylococcus aureus.
abstract: Some proportion of type A blaZ gene-positive methicillin-susceptible Staphylococcus aureus strains exhibit the cefazolin inoculum effect (CIE). The type A blaZ gene was divided into two groups by single nucleotide polymorphisms (SNPs) at Ser226Pro and Cys229Tyr. The median cefazolin MICs at a high inoculum concentration were 5.69 μg/ml for the Ser-Cys group and 40.32 μg/ml for the Pro-Tyr group (P = 0.01). The SNPs at codons 226 and 229 in the amino acid sequence encoded by the blaZ gene were closely associated with the CIE.
authors: Department of Internal Medicine, Pusan National University School of Medicine and Medical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.
authors: Sun Hee
authors: SH
authors: Lee
authors: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors: Wan Beom
authors: WB
authors: Park
authors: Department of Internal Medicine, Pusan National University School of Medicine and Medical Research Institute, Pusan National University Hospital, Busan, Republic of Korea ebenezere.lee@gmail.com.
authors: Shinwon
authors: 0000-0001-7652-7093
authors: S
authors: Lee
authors: Department of Internal Medicine, Pusan National University School of Medicine and Medical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.
authors: Sohee
authors: S
authors: Park
authors: Department of Internal Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
authors: Shin Woo
authors: SW
authors: Kim
authors: Department of Internal Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
authors: Jong-Myung
authors: JM
authors: Lee
authors: Department of Internal Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea.
authors: Hyun Ha
authors: HH
authors: Chang
authors: Department of Internal Medicine, Daegu Fatima Hospital, Daegu, Republic of Korea.
authors: Ki Tae
authors: KT
authors: Kwon
authors: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors: Pyoeng Gyun
authors: PG
authors: Choe
authors: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors: Nam Joong
authors: NJ
authors: Kim
authors: Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Republic of Korea.
authors: Hong Bin
authors: 0000-0001-6262-372X
authors: HB
authors: Kim
authors: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors: Myoung-Don
authors: MD
authors: Oh
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002437:Cefazolin / Q000494:pharmacology*; D003545:Cysteine / Q000235:genetics; D004151:Dipeptides / Q000235:genetics; D006801:Humans; D008712:Methicillin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D012694:Serine / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004151:Dipeptides; C074016:prolyl-tyrosine; D012694:Serine; D002437:Cefazolin; D003545:Cysteine; D008712:Methicillin