title: Three new alkaloids from Veratrum grandiflorum Loes with inhibition activities on Hedgehog pathway.
abstract: Three new steroidal alkaloids 1-3, together with four known compounds 4-7, were isolated from the ethanol extract of Veratrum grandiflorum Loes. Their structures were elucidated by NMR (1D and 2D NMR) and MS spectroscopic data. The inhibition activities on Hedgehog (Hh) pathway were evaluated using a cell-based bioassay system (Shh-LIGHT 2 cells). The results showed that compounds 1-3 and 5 displayed inhibitory activities obviously with the IC50 values of 0.63-3.11μM. Among them, compound 5 showed the most prominent inhibition activity (IC50=0.63±0.02μM). Thus, these active alkaloids may be potent natural compounds as Hh pathway inhibitors for the treatment of various cancers.
authors: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China. Electronic address: gaolijuan04141002@126.com.
authors: Lijuan
authors: L
authors: Gao
authors: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China. Electronic address: chenfy_hz@126.com.
authors: Fengyang
authors: F
authors: Chen
authors: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China. Electronic address: 13355811895@189.cn.
authors: Xiaoyu
authors: X
authors: Li
authors: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China. Electronic address: xsfdty@163.com.
authors: Shifang
authors: S
authors: Xu
authors: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China. Electronic address: cyj@zju.edu.cn.
authors: Wenhai
authors: W
authors: Huang
authors: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China. Electronic address: yeyiping2005@163.com.
authors: Yiping
authors: Y
authors: Ye
mesh_terms: D002460:Cell Line; D053823:Hedgehog Proteins / Q000037:antagonists & inhibitors*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D021241:Spectrometry, Mass, Electrospray Ionization; D014703:Veratrum / Q000737:chemistry*; D014704:Veratrum Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D053823:Hedgehog Proteins; D014704:Veratrum Alkaloids
keywords: Alkaloids; Cancers; Hedgehog pathway; Shh-LIGHT 2 cells; Veratrum grandiflorum Loes