title: Baicalin attenuates lipopolysaccharide induced inflammation and apoptosis of cow mammary epithelial cells by regulating NF-κB and HSP72.
abstract: Baicalin is the main ingredient of traditional Chinese herbal medicine, Scutellaria baicalensis, which has been widely used clinically as an anti-inflammatory agent. However, molecular mechanism of action of this drug is not yet clear. In the present study, the protective mechanism of baicalin against lipopolysaccharide (LPS) induced inflammatory injury in cow mammary epithelial cells (CMECs) was explored. For this purpose, in vitro cultured CMECs were treated with baicalin (10μg/mL) and LPS (10μg/mL) for 24 and 12h, respectively, and the cell viability was measured by using cell counting kit-8 (CCK-8). The results revealed that LPS induced inflammatory responses, as p-p65/p65 and p-IκBα/IκBα ratios and TNF-α and IL-1β production was increased in the CMECs. Both Bcl-2/Bax ratio and cell viability were decreased and caspase-3 cleaved following LPS treatment, indicating apoptosis of CMECs. Moreover, both LPS and baicalin increased HSP72 expression of the CMECs. However, cellular inflammatory responses and apoptosis were significantly reduced in baicalin treated CMECs. In conclusion, baicalin ameliorated inflammation and apoptosis of the CMECs induced by LPS via inhibiting NF-κB activation and up regulation of HSP72.
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China. Electronic address: dongdongjianchi@163.com.
authors: Wenhao
authors: W
authors: Yang
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China.
authors: Huatao
authors: H
authors: Li
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China.
authors: Xia
authors: X
authors: Cong
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China.
authors: Xin
authors: X
authors: Wang
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China.
authors: Zhongling
authors: Z
authors: Jiang
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China.
authors: Qian
authors: Q
authors: Zhang
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China.
authors: Xiaonan
authors: X
authors: Qi
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China.
authors: Shansong
authors: S
authors: Gao
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China.
authors: Rongfeng
authors: R
authors: Cao
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong Province, China. Electronic address: wrtian@126.com.
authors: Wenru
authors: W
authors: Tian
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002417:Cattle; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D050884:HSP72 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides; D008321:Mammary Glands, Animal / Q000166:cytology; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D050884:HSP72 Heat-Shock Proteins; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C038044:baicalin
keywords: Apoptosis; Baicalin; Cow mammary epithelial cells; HSP72; Inflammation; Lipopolysaccharide; NF-κB