title: IgG Binding Characteristics of Rhesus Macaque FcγR.
abstract: Indian rhesus macaques (Macaca mulatta) are routinely used in preclinical studies to evaluate therapeutic Abs and candidate vaccines. The efficacy of these interventions in many cases is known to rely heavily on the ability of Abs to interact with a set of Ab FcγR expressed on innate immune cells. Yet, despite their presumed functional importance, M. mulatta Ab receptors are largely uncharacterized, posing a fundamental limit to ensuring accurate interpretation and translation of results from studies in this model. In this article, we describe the binding characteristics of the most prevalent allotypic variants of M. mulatta FcγR for binding to both human and M. mulatta IgG of varying subclasses. The resulting determination of the affinity, specificity, and glycan sensitivity of these receptors promises to be useful in designing and evaluating studies of candidate vaccines and therapeutic Abs in this key animal model and exposes significant evolutionary divergence between humans and macaques.
authors: Thayer School of Engineering, Dartmouth College, Hanover, NH 03755;
authors: Ying N
authors: YN
authors: Chan
authors: Thayer School of Engineering, Dartmouth College, Hanover, NH 03755;
authors: Austin W
authors: 0000-0003-2916-6744
authors: AW
authors: Boesch
authors: Molecular and Cellular Biology Program, Dartmouth College, Hanover, NH 03755;
authors: Nana Y
authors: NY
authors: Osei-Owusu
authors: Thayer School of Engineering, Dartmouth College, Hanover, NH 03755;
authors: Ali
authors: A
authors: Emileh
authors: Molecular and Cellular Biology Program, Dartmouth College, Hanover, NH 03755;
authors: Andrew R
authors: AR
authors: Crowley
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115;
authors: Sarah L
authors: 0000-0002-0165-7587
authors: SL
authors: Cocklin
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115;
authors: Samantha L
authors: 0000-0002-3460-6428
authors: SL
authors: Finstad
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115;
authors: Caitlyn H
authors: CH
authors: Linde
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115;
authors: Rebecca A
authors: 0000-0001-9136-0738
authors: RA
authors: Howell
authors: Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102;
authors: Isaac
authors: 0000-0001-9828-271X
authors: I
authors: Zentner
authors: Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102;
authors: Simon
authors: 0000-0002-9074-741X
authors: S
authors: Cocklin
authors: Wasatch Microfluidics, Salt Lake City, UT 84103; and.
authors: Adam R
authors: 0000-0002-1734-4171
authors: AR
authors: Miles
authors: Wasatch Microfluidics, Salt Lake City, UT 84103; and.
authors: Joshua W
authors: 0000-0001-8981-8762
authors: JW
authors: Eckman
authors: Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139.
authors: Galit
authors: G
authors: Alter
authors: Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115;
authors: Joern E
authors: 0000-0001-8803-0922
authors: JE
authors: Schmitz
authors: Thayer School of Engineering, Dartmouth College, Hanover, NH 03755; Molecular and Cellular Biology Program, Dartmouth College, Hanover, NH 03755; margaret.e.ackerman@dartmouth.edu.
authors: Margaret E
authors: 0000-0002-4253-3476
authors: ME
authors: Ackerman
mesh_terms: D000818:Animals; D001665:Binding Sites; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008253:Macaca mulatta; D011961:Receptors, Fc / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D007074:Immunoglobulin G; D011961:Receptors, Fc