title: The construction of three-dimensional composite fibrous macrostructures with nanotextures for biomedical applications.
abstract: The development of modern biomedical nanotechnology requires three-dimensional macrostructures with nanotextures to meet the requirements for practical applications in intricate biological systems. Additionally, the restoration and regeneration of some specific body tissues and organs rely on the function of conductive polymers, which can provide electrical cues for cells. In this study, we fabricated three-dimensional composite nanofibre macrostructures of polycaprolactone (PCL) with different concentrations of polyaniline (PANi) by employing an improved electrospinning technology with a specially designed collector. The 3D structures possessed cap-like macrostructures with centimetre-scale thickness and interconnected pore nanotextures with nanometre-scale nanofibres. To estimate the biocompatibility of the 3D PCL/PANi composite nanofibre macrostructures, mouse myoblasts (C2C12 cells) were cultured as model cells. The initial responses of C2C12 cells to the 3D PCL/PANi composite macrostructures were significantly superior to those to pure PCL, that is, the cells exhibited typical myoblast-like morphologies with obvious pseudopodia and the moderate incorporation (less than 2.0 wt%) of conductive PANi facilitated cell proliferation, which indicated that PANi has appreciable cell affinity. Moreover, the addition of conductive PANi to the 3D composite nanofibre macrostructures considerably enhanced myoblast differentiation and myotube maturation. These results suggest that electrospun 3D PCL/PANi composite nanofibre macrostructures would have promising applications in tissue engineering.
authors: National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510641, People's Republic of China. Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors: Juqing
authors: J
authors: Song
authors: Huichang
authors: H
authors: Gao
authors: Guanglin
authors: G
authors: Zhu
authors: Xiaodong
authors: X
authors: Cao
authors: Xuetao
authors: X
authors: Shi
authors: Yingjun
authors: Y
authors: Wang
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000473:pathology; D032446:Myoblasts / Q000166:cytology; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000814:Aniline Compounds; D001672:Biocompatible Materials; D011091:Polyesters; C416807:polyaniline; C016240:polycaprolactone