title: Experiences and Factors that Influence Potassium Hydroxide Examination by Microscopists.
abstract: BACKGROUND
  Potassium hydroxide（KOH）examination is commonly used in dermatological practice. Despite its simplicity, rapidity, and minimal invasiveness, experience in specimen collection, preparation, and interpretation is extremely important.
  
  
  OBJECTIVE
  To determine the ability to interpret KOH examination of six microscopists with different levels of experience within the Department of Dermatology.
  
  
  METHODS
  Six volunteer microscopists, who have different experiences in KOH examination in terms of specimens per week（SPW）, were assigned to prepare and examine 10 unknown slides of skin scrapings. All participants were then paired into three groups and exchanged the slides set to their partner in each group for a second round of slides interpretation.
  
  
  RESULTS
  Results of examinations were classified as correct, false negative, false positive, and misinterpretation. The highly experienced microscopists achieved more correct answers than the fairly experienced group in both sessions. There was a significant positive correlation between SPW（r=1.0, Spearman rank, p=0.01）and the correct answers; and a significant negative correlation between SPW and misinterpretation（r= -1.0, Spearman rank, p<0.01）, exclusively for the second session.
  
  
  CONCLUSIONS
  A small number of volunteer microscopists was enrolled in this study.
  
  
  CONCLUSIONS
  Experience in routine slide examination and time spent during examination were significant factors for accurate interpretation of KOH examination. Positive correlation between experience and correct answers, and negative correlation between experience and misinterpretation were particularly observed under limited examination time.
authors: Department of Dermatology, Faculty of Medicine Siriraj Hospital Mahidol University.
authors: Sumanas
authors: S
authors: Bunyaratavej
authors: Penvadee
authors: P
authors: Pattanaprichakul
authors: Sorachai
authors: S
authors: Srisuma
authors: Charussri
authors: C
authors: Leeyaphan
mesh_terms: D003880:Dermatology / Q000379:methods*; D003881:Dermatomycoses / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D006878:Hydroxides; D008853:Microscopy; D009172:Mycology / Q000379:methods*; D017680:Potassium Compounds; D011361:Professional Competence; D013048:Specimen Handling
chemical_list: D006878:Hydroxides; D017680:Potassium Compounds; C029943:potassium hydroxide