title: H4K5 histone acetylation ofBRG1is associated with heroin administration rather than addiction.
abstract: Diacetylmorphine hydrochloride (heroin) addiction is a chronic relapsing brain disorder that is a heavy public health burden worldwide. Brm/SWI2-related gene-1 (BRG1) is a tumor suppressor gene that can influence embryogenesis and the development of the cerebellum. The current study aimed to investigate the effect of histone H4 lysine 5 (H4K5) modifications on theBRG1gene in brain tissue of the ventral tegmental area (VTA) of heroin-addicted rats. A total of 21 male Sprague Dawley rats were raised in a standard manner and underwent heroin self-administration training. Rats were randomly divided into three equal groups: Group A, self-administered delivery of heroin; group B, yoked delivery of heroin; and group C, yoked delivery of saline. The VTA was harvested and subjected to chromatin immunoprecipitation (ChIP) analysis. Gene expression was evaluated by quantitative polymerase chain reaction. We calculated the recovery rate, which indicated the percentage of the total inputBRG1recovered by ChIP. Our results showed thatBRG1was less associated with H4K5 histone modification in the group of rats that underwent heroin self-administration than in the other two groups (A vs. B, P=0.031; A vs. C, P=0.067). The recovery fold changes of the self-administration group and the passive-administration group were significantly different from those of the group with yoked saline (A vs. C, P=0.013; B vs. C, P=0.009; A vs. B, P=0.731). The results of the current study demonstrated that H4K5 histone acetylation ofBRG1in the VTA may be associated with heroin administration, but not addiction.
authors: Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, Zhejiang 315211, P.R. China; Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou, Zhejiang 313000, P.R. China.
authors: Limin
authors: L
authors: Xu
authors: Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, Zhejiang 315211, P.R. China.
authors: Qingxiao
authors: Q
authors: Hong
authors: Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, Zhejiang 315211, P.R. China.
authors: Xiaoying
authors: X
authors: Chen
authors: Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, Zhejiang 315211, P.R. China.
authors: Xuting
authors: X
authors: Xu
authors: Laboratory of Behavioral Neuroscience, Ningbo University, Ningbo, Zhejiang 315010, P.R. China; Ningbo Institute of Microcirculation and Henbane, Ningbo, Zhejiang 315010, P.R. China.
authors: Huifen
authors: H
authors: Liu
authors: Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, Zhejiang 315211, P.R. China; Laboratory of Behavioral Neuroscience, Ningbo University, Ningbo, Zhejiang 315010, P.R. China; Ningbo Institute of Microcirculation and Henbane, Ningbo, Zhejiang 315010, P.R. China.
authors: Wenhua
authors: W
authors: Zhou
authors: Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, Zhejiang 315211, P.R. China.
authors: Shiwei
authors: S
authors: Duan
keywords: chromatin immunoprecipitation; heroin addiction; histone acetylation; ventral tegmental area