title: Synthesis of Mg-Fe-Cl hydrotalcite-like nanoplatelets as an oral phosphate binder: evaluations of phosphorus intercalation activity and cellular cytotoxicity.
abstract: The patients with end-stage of renal disease (ESRD) need to take oral phosphate binder. Traditional phosphate binders may leave the disadvantage of aluminum intoxication or cardiac calcification. Herein, Mg-Fe-Cl hydrotalcite-like nanoplatelet (HTln) is for the first time characterized as potential oral phosphate binder, with respect to its phosphorus uptake capacity in cow milk and cellular cytotoxicity. A novel method was developed for synthesizing the Mg-Fe-Cl HTln powder in different Mg(2+): Fe(3+) ratios where the optimization was 2.8:1. Addition of 0.5 g Mg-Fe-Cl HTln in cow milk could reduce its phosphorus content by 40% in 30 min and by 65% in 90 min. In low pH environment, the Mg-Fe-Cl HTln could exhibit relatively high performance for uptaking phosphorus. During a 90 min reaction of the HTln in milk, no phosphorus restoration occurred. In-vitro cytotoxicity assay of Mg-Fe-Cl HTln revealed no potential cellular cytotoxicity. The cells that were cultured in the HTln extract-containing media were even more viable than cells that were cultured in extract-free media (blank control). The Mg-Fe-Cl HTln extract led to hundred ppm of Mg ion and some ppm of Fe ion in the media, should be a positive effect on the good cell viability.
authors: Department of Materials Science and Engineering, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung 402, Taiwan, ROC.
authors: Yung-Feng
authors: YF
authors: Lung
authors: Department of Dentistry, National Yang-Ming University, 155, Sec.2, Linong Street, Taipei 112, Taiwan, ROC.
authors: Ying-Sui
authors: YS
authors: Sun
authors: Green Energy and Environment Research Laboratories, Industrial Technology Research Institute, 195 Sec. 4, Chung-Hsing Road, Hsin-Chu 31040, Taiwan, ROC.
authors: Chun-Kai
authors: CK
authors: Lin
authors: Department of Materials Science and Engineering, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung 402, Taiwan, ROC.
authors: Jun-Yen
authors: JY
authors: Uan
authors: Department of Dentistry, National Yang-Ming University, 155, Sec.2, Linong Street, Taipei 112, Taiwan, ROC.|Department of Bioinformatics and Medical Engineering, Asia University, 500 Lioufeng Rd., Wufeng, Taichung 413, Taiwan 500, ROC.|Department of Medical Research, China Medical University Hospital, 2 Yude Rd., Taichung 407, Taiwan, ROC.
authors: Her-Hsiung
authors: HH
authors: Huang
mesh_terms: D000327:Adsorption; D000536:Aluminum Hydroxide / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000628:therapy; D007700:Kinetics; D008276:Magnesium Hydroxide / Q000737:chemistry*; D051379:Mice; D008892:Milk / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry / Q000302:isolation & purification*; D010758:Phosphorus / Q000737:chemistry / Q000302:isolation & purification*; D006435:Renal Dialysis; D012996:Solutions
chemical_list: D010710:Phosphates; D012996:Solutions; C010467:hydrotalcite; D010758:Phosphorus; D000536:Aluminum Hydroxide; D008276:Magnesium Hydroxide