title: Layered chitosan-collagen hydrogel/aligned PLLA nanofiber construct for flexor tendon regeneration.
abstract: The aim of our study was to develop a tendon construct of electrospun aligned poly (l-lactic acid) (PLLA) nanofibers, to mimic the aligned collagen fiber bundles and layering PLLA fibers with chitosan-collagen hydrogel, to mimic the glycosaminoglycans of sheath ECM for tendon regeneration. The hydrogel coated electrospun membrane was rolled and an outer coating of alginate gel was given to prevent peritendinous adhesion. The developed constructs were characterized by SEM, FT-IR and tensile testing. Protein adsorption studies showed lower protein adsorption on coated scaffolds compared to uncoated scaffolds. The samples were proven to be non-toxic to tenocytes. The chitosan-collagen/PLLA uncoated scaffolds and alginate gel coated chitosan-collagen/PLLA scaffolds showed good cell proliferation. The tenocytes showed good attachment and spreading on the scaffolds. This study indicated that the developed chitosan-collagen/PLLA/alginate scaffold would be suitable for flexor tendon regeneration.
authors: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Amrita University, Kochi 682041, India.
authors: S
authors: S
authors: Deepthi
authors: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Amrita University, Kochi 682041, India.
authors: M
authors: M
authors: Nivedhitha Sundaram
authors: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Amrita University, Kochi 682041, India.
authors: J
authors: J
authors: Deepti Kadavan
authors: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Amrita University, Kochi 682041, India. Electronic address: rjayakumar@aims.amrita.edu.
authors: R
authors: R
authors: Jayakumar
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry*; D011817:Rabbits; D012038:Regeneration; D013710:Tendons / Q000166:cytology / Q000502:physiology*; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C033616:poly(lactide); D003094:Collagen; D048271:Chitosan
keywords: Alginate; Chitosan-collagen hydrogel; PLLA nanofibers; Peritendinous adhesion; Tendon regeneration