title: DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization.
abstract: Construction and physico-chemical behavior of DNA three way junction (3WJ) functionalized by protein-like residues (imidazole, alcohol and carboxylic acid) at unpaired positions at the core is described. One 5'-C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react through a post synthetic CuACC reaction with either protected imidazolyl-, hydroxyl- or carboxyl-azide. Structural impacts of 5'-C(S)-functionalization were investigated to evaluate how 3WJ flexibility/stability is affected.
authors: Laboratoire de Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique, UMR CNRS 5068, Université Paul Sabatier, 118 Route de Narbonne, Cedex 9, Toulouse 31062, France. addamiano@chimie.ups-tlse.fr.
authors: Claudia
authors: C
authors: Addamiano
authors: Laboratoire de Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique, UMR CNRS 5068, Université Paul Sabatier, 118 Route de Narbonne, Cedex 9, Toulouse 31062, France. gerland@chimie.ups-tlse.fr.
authors: Béatrice
authors: B
authors: Gerland
authors: Laboratoire de Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique, UMR CNRS 5068, Université Paul Sabatier, 118 Route de Narbonne, Cedex 9, Toulouse 31062, France. payrastr@chimie.ups-tlse.fr.
authors: Corinne
authors: C
authors: Payrastre
authors: Laboratoire de Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique, UMR CNRS 5068, Université Paul Sabatier, 118 Route de Narbonne, Cedex 9, Toulouse 31062, France. escudier@chimie.ups-tlse.fr.
authors: Jean-Marc
authors: JM
authors: Escudier
mesh_terms: D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA
keywords: CuACC; chemically modified oligonucleotides; convertible nucleotide; three way junction