title: Identification of BCL11B as a regulator of adipogenesis.
abstract: The differentiation of preadipocytes into adipocytes is controlled by several transcription factors, including peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), which are known as master regulators of adipogenesis. BCL11B is a zinc finger-type transcription factor that regulates the development of the skin and central nervous and immune systems. Here, we found that BCL11B was expressed in the white adipose tissue (WAT), particularly the subcutaneous WAT and that BCL11B(-/-) mice had a reduced amount of subcutaneous WAT. During adipogenesis, BCL11B expression transiently increased in 3T3-L1 preadipocytes and mouse embryonic fibroblasts (MEFs). The ability for adipogenesis was reduced in BCL11B knockdown 3T3-L1 cells and BCL11B(-/-) MEFs, whereas the ability for osteoblastogenesis was unaffected in BCL11B(-/-) MEFs. Luciferase reporter gene assays revealed that BCL11B stimulated C/EBPβ activity. Furthermore, the expression of downstream genes of the Wnt/β-catenin signaling pathway was not suppressed in BCL11B(-/-) MEFs during adipogenesis. Thus, this study identifies BCL11B as a novel regulator of adipogenesis, which works, at least in part, by stimulating C/EBPβ activity and suppressing the Wnt/β-catenin signaling pathway.
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Jun
authors: J
authors: Inoue
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Yusuke
authors: Y
authors: Ihara
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Daisuke
authors: D
authors: Tsukamoto
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Keisuke
authors: K
authors: Yasumoto
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.|Institute of Gerontology, The University of Tokyo, Tokyo, Japan.
authors: Tsutomu
authors: T
authors: Hashidume
authors: Division of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
authors: Kenya
authors: K
authors: Kamimura
authors: Institute for Food Sciences, Hirosaki University, Aomori, Japan.
authors: Yuji
authors: Y
authors: Nakai
authors: Department of Medical Technology, School of Health Sciences, Faculty of Medicine, Niigata University, Niigata, Japan.
authors: Shigeki
authors: S
authors: Hirano
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Makoto
authors: M
authors: Shimizu
authors: Division of Molecular Biology, Department of Molecular Genetics, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
authors: Ryo
authors: R
authors: Kominami
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Ryuichiro
authors: R
authors: Sato
mesh_terms: D017667:Adipocytes / Q000502:physiology; D050156:Adipogenesis; D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000096:biosynthesis*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D051379:Mice; D018345:Mice, Knockout; D012097:Repressor Proteins / Q000172:deficiency / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000502:physiology*; D025521:Tumor Suppressor Proteins / Q000172:deficiency / Q000378:metabolism*; D060449:Wnt Signaling Pathway
chemical_list: C511143:Bcl11b protein, mouse; D022782:CCAAT-Enhancer-Binding Protein-beta; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins