title: New insight into determining indicators of metabolic status in women: Expression of PPARγ and FABP4 in PBMCs.
abstract: Adiposity and its metabolic disturbances could be regulated by adipocyte-specific peroxisome proliferator-activated receptor gamma and fatty acid-binding protein4. Although these two proteins are mainly expressed in adipose tissues, they can also be expressed in peripheral blood mononuclear cells, which could be useful for predicting body composition and blood parameters. Thus, this cross-sectional study was performed during January 2013-January 2014 with 229 women (age range, 22-52 years) who were classified as obese or nonobese. Serum glucose, insulin, lipids, and body composition were measured in the fasting state. Peripheral blood mononuclear cells were isolated to extract ribonucleic acid (RNA) and to determine gene expression by real time polymerase chain reaction (PCR). All serum parameters and components of body composition were significantly higher in obese than in nonobese women. Gene expression analysis showed that serum levels of glucose and lipids, except high-density lipoprotein (HDL), were higher in the group that expressed high fatty acid-binding protein4. Increased expression of the peroxisome proliferator-activated receptor gamma was associated with a significant reduction of blood sugar and increased HDL and other lipids and visceral fat. Therefore, it seems that the level of expression of these genes in peripheral blood mononuclear cells may indicate metabolic status.
authors: a Department of Community Nutrition, School of Nutritional Sciences and Dietetics , Tehran University of Medical Sciences (TUMS) , Tehran , Iran.
authors: Negar
authors: N
authors: Zamaninour
authors: a Department of Community Nutrition, School of Nutritional Sciences and Dietetics , Tehran University of Medical Sciences (TUMS) , Tehran , Iran.
authors: Khadijeh
authors: K
authors: Mirzaei
authors: b Department of Clinical Nutrition, School of Nutritional Sciences and Dietetics , Tehran University of Medical Sciences (TUMS) , Tehran , Iran.
authors: Seyed Ali
authors: SA
authors: Keshavarz
authors: c Osteoporosis Research Center, Endocrinology and Metabolism Clinical Sciences Institute , Tehran University of Medical Sciences (TUMS) , Tehran , Iran.
authors: Hastimansooreh
authors: H
authors: Ansar
authors: c Osteoporosis Research Center, Endocrinology and Metabolism Clinical Sciences Institute , Tehran University of Medical Sciences (TUMS) , Tehran , Iran.|d Department of Medicine, Section of Endocrinology, Nutrition, and Diabetes, Vitamin D, Skin, and Bone Research Laboratory , Boston University Medical Center , Boston , Massachusetts , USA.
authors: Arash
authors: A
authors: Hossein-Nezhad
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition / Q000235:genetics*; D015992:Body Mass Index; D003430:Cross-Sectional Studies; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007328:Insulin / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008055:Lipids / Q000097:blood; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D001786:Blood Glucose; C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D007328:Insulin; D008055:Lipids; D047495:PPAR gamma
keywords: Adipose tissue; body composition; body mass index; metabolic disorders; obesity; visceral fat