title: Lack of Overt Genome Reduction in the Bryostatin-Producing Bryozoan Symbiont "Candidatus Endobugula sertula".
abstract: The uncultured bacterial symbiont "Candidatus Endobugula sertula" is known to produce cytotoxic compounds called bryostatins, which protect the larvae of its host, Bugula neritina The symbiont has never been successfully cultured, and it was thought that its genome might be significantly reduced. Here, we took a shotgun metagenomics and metatranscriptomics approach to assemble and characterize the genome of "Ca Endobugula sertula." We found that it had specific metabolic deficiencies in the biosynthesis of certain amino acids but few other signs of genome degradation, such as small size, abundant pseudogenes, and low coding density. We also identified homologs to genes associated with insect pathogenesis in other gammaproteobacteria, and these genes may be involved in host-symbiont interactions and vertical transmission. Metatranscriptomics revealed that these genes were highly expressed in a reproductive host, along with bry genes for the biosynthesis of bryostatins. We identified two new putative bry genes fragmented from the main bry operon, accounting for previously missing enzymatic functions in the pathway. We also determined that a gene previously assigned to the pathway, bryS, is not expressed in reproductive tissue, suggesting that it is not involved in the production of bryostatins. Our findings suggest that "Ca Endobugula sertula" may be able to live outside the host if its metabolic deficiencies are alleviated by medium components, which is consistent with recent findings that it may be possible for "Ca Endobugula sertula" to be transmitted horizontally.
  
  
  
  The bryostatins are potent protein kinase C activators that have been evaluated in clinical trials for a number of indications, including cancer and Alzheimer's disease. There is, therefore, considerable interest in securing a renewable supply of these compounds, which is currently only possible through aquaculture of Bugula neritina and total chemical synthesis. However, these approaches are labor-intensive and low-yielding and thus preclude the use of bryostatins as a viable therapeutic agent. Our genome assembly and transcriptome analysis for "Ca Endobugula sertula" shed light on the metabolism of this symbiont, potentially aiding isolation and culturing efforts. Our identification of additional bry genes may also facilitate efforts to express the complete pathway heterologously.
authors: Pharmaceutical Sciences Division, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors: Ian J
authors: IJ
authors: Miller
authors: Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, Virginia, USA.
authors: Niti
authors: N
authors: Vanee
authors: Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, Virginia, USA.
authors: Stephen S
authors: SS
authors: Fong
authors: Department of Biology, Randolph-Macon College, Ashland, Virginia, USA.
authors: Grace E
authors: GE
authors: Lim-Fong
authors: Pharmaceutical Sciences Division, University of Wisconsin-Madison, Madison, Wisconsin, USA jason.kwan@wisc.edu.
authors: Jason C
authors: 0000-0001-9933-1536
authors: JC
authors: Kwan
mesh_terms: D000818:Animals; D054713:Bryostatins / Q000096:biosynthesis*; D002013:Bryozoa / Q000382:microbiology*; D020563:Gammaproteobacteria / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D016680:Genome, Bacterial; D007814:Larva / Q000382:microbiology; D056186:Metagenomics; D010802:Phylogeny; D011544:Pseudogenes; D013559:Symbiosis
chemical_list: D054713:Bryostatins; C046785:bryostatin 1