title: Gonococcal Antimicrobial Susceptibility and the Prevalence of blaTEM-1and blaTEM-135Genes in Neisseria gonorrhoeae Isolates from Thailand.
abstract: We studied the antimicrobial susceptibility and prevalence of the blaTEM-1and blaTEM-135genes in Neisseria gonorrhoeae isolates obtained in Thailand. The isolates were tested using the disk diffusion method, and 100% of 370 isolates were found susceptible to cefixime, ceftriaxone, cefotaxime, ceftazidime, cefepime, spectinomycin, and azithromycin. Some of the isolates were resistant to penicillin (85.7%), ciprofloxacin (88.0%), ofloxacin (97.4%), or tetracycline (89.1%). Penicillinase-producing N. gonorrhoeae accounted for 83.8% of isolates, with 70.0% of these further identified as penicillinase-producing plus tetracycline resistant N. gonorrhoeae. Penicillin, tetracycline, and ciprofloxacin are not recommended for treatment because of the high prevalence (89.7%) of multidrug resistant gonococci. A study of genes controlling enzyme of beta-lactamase production (blaTEM-1and blaTEM-135) was performed using mismatch amplification mutation assay PCR method and DNA sequencing. Beta-lactamase positive N. gonorrhoeae carried blaTEM-1(69.6%) and blaTEM-135(30.4%), indicating that there is a significant increase and spread of blaTEM-135among gonococci in Thailand.
authors: Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University.
authors: Chanwit
authors: C
authors: Tribuddharat
authors: Pintip
authors: P
authors: Pongpech
authors: Angkana
authors: A
authors: Charoenwatanachokchai
authors: Somchai
authors: S
authors: Lokpichart
authors: Somporn
authors: S
authors: Srifuengfung
authors: Suthatta
authors: S
authors: Sonprasert
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D052978:Disk Diffusion Antimicrobial Tests; D024881:Drug Resistance, Bacterial; D005260:Female; D006069:Gonorrhea / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017422:Sequence Analysis, DNA; D013785:Thailand / Q000453:epidemiology; D055815:Young Adult; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases