title: A novel monopartite dsRNA virus isolated from the entomopathogenic and nematophagous fungus Purpureocillium lilacinum.
abstract: Purpureocillium lilacinum is a ubiquitous saprophytic fungus commonly isolated from soils and widely known as a biological control agent against phytopathogenic nematodes and pest insects. Mycoviruses infect a wide number of fungal species, but the study of viruses infecting entomopathogenic fungi is still quite recent. In this study, a total of 86 P. lilacinum isolates collected from soil in natural and cultivated habitats throughout the Czech Republic were analyzed; 22 % of the isolates harbored double-stranded RNA (dsRNA) elements with viral characteristics. These results suggest that mycoviruses are common in P. lilacinum. One of the most common dsRNA elements detected in the survey was completely sequenced and corresponded to the 2,864-bp genome of a previously undescribed mycovirus, designated Purpureocillium lilacinum nonsegmented virus 1 (PlNV-1). Phylogenetic analysis of the RNA-dependent RNA polymerase of PlNV-1 indicated that this virus might belong to a new taxon related to the family Partitiviridae.
authors: Laboratory of Entomopathogenic Nematodes, Institute of Entomology, Biology Centre, The Czech Academy of Sciences, Branišovská 31/1160, 37005, České Budějovice, Czech Republic. noemihas@gmail.com.
authors: Noemi
authors: http://orcid.org/0000-0002-2473-5330
authors: N
authors: Herrero
mesh_terms: D016000:Cluster Analysis; D018153:Czech Republic; D000067148:Fungal Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral; D006999:Hypocreales / Q000302:isolation & purification / Q000821:virology*; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012988:Soil Microbiology
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral