title: Pseudomonas aeruginosa ATCC 9027 is a non-virulent strain suitable for mono-rhamnolipids production.
abstract: Rhamnolipids produced by Pseudomonas aeruginosa are biosurfactants with a high biotechnological potential, but their extensive commercialization is limited by the potential virulence of P. aeruginosa and by restrictions in producing these surfactants in heterologous hosts. In this work, we report the characterization of P. aeruginosa strain ATCC 9027 in terms of its genome-sequence, virulence, antibiotic resistance, and its ability to produce mono-rhamnolipids when carrying plasmids with different cloned genes from the type strain PAO1. The genes that were expressed from the plasmids are those coding for enzymes involved in the synthesis of this biosurfactant (rhlA and rhlB), as well as the gene that codes for the RhlR transcriptional regulator. We confirm that strain ATCC 9027 forms part of the PA7 clade, but contrary to strain PA7, it is sensitive to antibiotics and is completely avirulent in a mouse model. We also report that strain ATCC 9027 mono-rhamnolipid synthesis is limited by the expression of the rhlAB-R operon. Thus, this strain carrying the rhlAB-R operon produces similar rhamnolipids levels as PAO1 strain. We determined that strain ATCC 9027 with rhlAB-R operon was not virulent to mice. These results show that strain ATCC 9027, expressing PAO1 rhlAB-R operon, has a high biotechnological potential for industrial mono-rhamnolipid production.
authors: Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70228, 04510, México, DF, Mexico.
authors: María-Victoria
authors: MV
authors: Grosso-Becerra
authors: Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70228, 04510, México, DF, Mexico.
authors: Abigail
authors: A
authors: González-Valdez
authors: Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70228, 04510, México, DF, Mexico.
authors: María-Jessica
authors: MJ
authors: Granados-Martínez
authors: Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70228, 04510, México, DF, Mexico.
authors: Estefanía
authors: E
authors: Morales
authors: Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70228, 04510, México, DF, Mexico.
authors: Luis
authors: L
authors: Servín-González
authors: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, México, DF, Mexico.
authors: José-Luis
authors: JL
authors: Méndez
authors: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, México, DF, Mexico.
authors: Gabriela
authors: G
authors: Delgado
authors: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, México, DF, Mexico.
authors: Rosario
authors: R
authors: Morales-Espinosa
authors: Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, México, DF, Mexico.
authors: Gabriel-Yaxal
authors: GY
authors: Ponce-Soto
authors: Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70228, 04510, México, DF, Mexico.
authors: Miguel
authors: M
authors: Cocotl-Yañez
authors: Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70228, 04510, México, DF, Mexico. gloria@biomedicas.unam.mx.
authors: Gloria
authors: G
authors: Soberón-Chávez
mesh_terms: D000818:Animals; D003651:Decanoates / Q000378:metabolism*; D004195:Disease Models, Animal; D024881:Drug Resistance, Bacterial; D016680:Genome, Bacterial; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics; D051379:Mice; D009876:Operon; D010957:Plasmids; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D012210:Rhamnose / Q000031:analogs & derivatives* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013501:Surface-Active Agents / Q000378:metabolism*; D014774:Virulence
chemical_list: D003651:Decanoates; D013501:Surface-Active Agents; C085734:rhamnopyranosyl-3-hydroxydecanoyl-3-hydroxydecanoate; D012210:Rhamnose
keywords: Biosurfactant production; Metabolic engineering; Pseudomonas aeruginosa virulence; Quorum-sensing response