title: Structural characterization and inhibition on α-d-glucosidase activity of non-starch polysaccharides from Fagopyrum tartaricum.
abstract: In the present study, the crude polysaccharide was extracted from Fagopyrum tartaricum and purified by Sephadex G-25 and G-75 column to produce a polysaccharide fraction termed TBP-II. Its average molecular weight was 26kDa. The structural characterization of TBP-II was investigated by gas chromatography, periodate oxidation-Smith degradation, Methylation and NMR. Congo red was applied to explore its advanced structures. The results revealed that chemical composition and structural characteristic of TBP-II was mainly consisted of galactose, arabinose, xylose and glucose with a molar ratio of 0.7:1:6.3:74.2. The backbone of TBP-II was composed of (1→4)-linked α-d-glucopyranosyl (Glcp), while the branches comprised of (1→3)-linked α-d-glucopyranosyl (Glcp), (1→6)-linked α-d-galactopyranosyl (Galp) and (1→2,4)-linked α-d-rhamnopyranosyl (Rhap). The structure of TBP-II was 1,3 and 1,6-branched-galactorhamnoglucan that had a linear backbone of (1→4)-linked α-d-glucopyranose (Glcp). Using Congo red assay showed that it was absent of triple helix structure. The α-d-glucosidase inhibitory activity of TBP-II was determined using acarbose as positive control. The result showed that the inhibition rate depended on the concentration of polysaccharides.
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors: Xiao-Ting
authors: XT
authors: Wang
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, 300457 Tianjin, PR China. Electronic address: zhyuanzhu@tust.edu.cn.
authors: Zhen-Yuan
authors: ZY
authors: Zhu
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors: Liang
authors: L
authors: Zhao
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors: Hui-Qing
authors: HQ
authors: Sun
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors: Meng
authors: M
authors: Meng
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
authors: Jin-Yu
authors: JY
authors: Zhang
authors: Université Pierre et Marie Curie-Paris 6, Institut Parisien de Chimie Moléculaire, UMR CNRS 8232, 4 Place Jussieu, 75005 Paris, France.
authors: Yong-Min
authors: YM
authors: Zhang
mesh_terms: D020909:Acarbose / Q000494:pharmacology; D019612:Fagopyrum / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D009005:Monosaccharides / Q000032:analysis / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D009005:Monosaccharides; D011134:Polysaccharides; D000520:alpha-Glucosidases; D020909:Acarbose
keywords: Acetic acid (PubChem CID: 176); Butanol (PubChem CID: 263); Characterization; Chloroform (PubChem CID: 6212); Dimethyl sulfoxide (PubChem CID: 679); Ethanol (PubChem CID: 702); Fagopyrum tartaricum; Polysaccharide; Sodium hydroxide (PubChem CID:14798); Trifluoroacetic acid(PubChem CID: 6422); α-d-glucosidase