title: Selective synthesis of human milk fat-style structured triglycerides from microalgal oil in a microfluidic reactor packed with immobilized lipase.
abstract: Human milk fat-style structured triacylglycerols were produced from microalgal oil in a continuous microfluidic reactor packed with immobilized lipase for the first time. A remarkably high conversion efficiency was demonstrated in the microreactor with reaction time being reduced by 8 times, Michaelis constant decreased 10 times, the lipase reuse times increased 2.25-fold compared to those in a batch reactor. In addition, the content of palmitic acid at sn-2 position (89.0%) and polyunsaturated fatty acids at sn-1, 3 positions (81.3%) are slightly improved compared to the product in a batch reactor. The increase of melting points (1.7°C) and decrease of crystallizing point (3°C) implied higher quality product was produced using the microfluidic technology. The main cost can be reduced from $212.3 to $14.6 per batch with the microreactor. Overall, the microfluidic bioconversion technology is promising for modified functional lipids production allowing for cost-effective approach to produce high-value microalgal coproducts.
authors: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, PR China. Electronic address: wangjun@just.edu.cn.
authors: Jun
authors: J
authors: Wang
authors: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors: Xi
authors: X
authors: Liu
authors: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors: Xu-Dong
authors: XD
authors: Wang
authors: National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO, USA.
authors: Tao
authors: T
authors: Dong
authors: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors: Xing-Yu
authors: XY
authors: Zhao
authors: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors: Dan
authors: D
authors: Zhu
authors: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors: Yi-Yuan
authors: YY
authors: Mei
authors: School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, PR China. Electronic address: ghwu@just.edu.cn.
authors: Guo-Hua
authors: GH
authors: Wu
mesh_terms: D019149:Bioreactors; D003460:Crystallization; D019358:Fat Substitutes / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated; D008049:Lipase / Q000378:metabolism*; D058086:Microalgae / Q000378:metabolism*; D044085:Microfluidics / Q000379:methods; D008895:Milk, Human / Q000737:chemistry*; D008956:Models, Chemical; D019308:Palmitic Acid; D010938:Plant Oils / Q000378:metabolism*; D014280:Triglycerides / Q000096:biosynthesis*
chemical_list: D019358:Fat Substitutes; D005231:Fatty Acids, Unsaturated; D010938:Plant Oils; D014280:Triglycerides; D019308:Palmitic Acid; D008049:Lipase
keywords: Coproduct; Microalgae oil; Microfluidic bioconversion; Polyunsaturated fatty acid; Structured triacylglycerols