title: Nigella sativa Relieves the Altered Insulin Receptor Signaling in Streptozotocin-Induced Diabetic Rats Fed with a High-Fat Diet.
abstract: The black cumin (Nigella sativa) "NS" or the black seeds have many pharmacological activities such as antioxidant, anticarcinogenic, antihypertensive, and antidiabetic properties. In this work, streptozotocin-induced diabetic rats fed with a high-fat diet were treated daily with NS oil (NSO) in order to study the effect on the blood glucose, lipid profile, oxidative stress parameters, and the gene expression of some insulin receptor-induced signaling molecules. This treatment was combined also with some drugs (metformin and glimepiride) and the insulin receptor inhibitor I-OMe-AG538. The administration of NSO significantly induced the gene expression of insulin receptor compared to rats that did not receive NSO. Also, it upregulated the expression of insulin-like growth factor-1 and phosphoinositide-3 kinase, whereas the expression of ADAM-17 was downregulated. The expression of ADAM-17 is corroborated by the analysis of TIMP-3 content. In addition, the NSO significantly reduced blood glucose level, components of the lipid profile, oxidative stress parameters, serum insulin/insulin receptor ratio, and the tumor necrosis factor-α, confirming that NSO has an antidiabetic activity. Thus, the daily NSO treatment in our rat model indicates that NSO has a potential in the management of diabetes as well as improvement of insulin-induced signaling.
authors: Biochemistry Department, Faculty of Science, Alexandria University, Alexandria 21511, Egypt.
authors: Mahmoud
authors: 0000-0002-4406-882X
authors: M
authors: Balbaa
authors: Biochemistry Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
authors: Marwa
authors: M
authors: El-Zeftawy
authors: Biochemistry Department, Faculty of Science, Alexandria University, Alexandria 21511, Egypt.
authors: Doaa
authors: 0000-0003-3327-4377
authors: D
authors: Ghareeb
authors: Biochemistry Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
authors: Nabil
authors: N
authors: Taha
authors: Biochemistry Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
authors: Abdel Wahab
authors: AW
authors: Mandour
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000737:chemistry*; D003921:Diabetes Mellitus, Experimental; D059305:Diet, High-Fat; D008297:Male; D031881:Nigella sativa / Q000737:chemistry*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D011972:Receptor, Insulin / Q000737:chemistry*
chemical_list: D015703:Antigens, CD; C435941:INSR protein, human; D011972:Receptor, Insulin