title: Long Noncoding RNA HOXC-AS1 Suppresses Ox-LDL-Induced Cholesterol Accumulation Through Promoting HOXC6 Expression in THP-1 Macrophages.
abstract: Atherosclerosis is a common pathological basis of cardiovascular disease, which remains the leading cause of mortality. Long noncoding RNAs (lncRNAs) are newly studied non-protein-coding RNAs involved in gene regulation, but how lncRNAs exert regulatory effect on atherosclerosis remains unclear. In this study, we found that lncRNA HOXC cluster antisense RNA 1 (HOXC-AS1) and homeobox C6 (HOXC6) were downregulated in carotid atherosclerosis by performing microarray analysis. The results were verified in atherosclerotic plaques and normal arterial intima tissues by quantitative reverse transcription PCR and western blot analysis. Lentivirus-mediated overexpression of HOXC-AS1 induced HOXC6 expression at mRNA and protein levels in THP-1 macrophages. Besides, oxidized low-density lipoprotein (Ox-LDL) decreased expression of HOXC-AS1 and HOXC6 in a time-dependent manner. Induction of cholesterol accumulation by Ox-LDL could be partly suppressed by overexpression of HOXC-AS1.
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Chuan
authors: C
authors: Huang
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Yan-Wei
authors: YW
authors: Hu
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Jing-Jing
authors: JJ
authors: Zhao
authors: 2 Department of Anesthesiology, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Xin
authors: X
authors: Ma
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Yuan
authors: Y
authors: Zhang
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Feng-Xia
authors: FX
authors: Guo
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Chun-Min
authors: CM
authors: Kang
authors: 3 Department of Vascular Surgery, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Jing-Bo
authors: JB
authors: Lu
authors: 4 Department of Cardiology, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Jian-Cheng
authors: JC
authors: Xiu
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Yan-Hua
authors: YH
authors: Sha
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Ji-Juan
authors: JJ
authors: Gao
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Yan-Chao
authors: YC
authors: Wang
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Pan
authors: P
authors: Li
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Bang-Ming
authors: BM
authors: Xu
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Lei
authors: L
authors: Zheng
authors: 1 Laboratory Medicine Center, Nanfang Hospital, Southern Medical University , Guangzhou, China .
authors: Qian
authors: Q
authors: Wang
mesh_terms: D050197:Atherosclerosis / Q000378:metabolism; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C094289:HOXC6 protein, human; D018398:Homeodomain Proteins; D008077:Lipoproteins, LDL; D062085:RNA, Long Noncoding; C099252:oxidized low density lipoprotein; D002784:Cholesterol
keywords: HOXC-AS1; HOXC6; Ox-LDL; cholesterol; lncRNA