title: Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible.
abstract: Observing changes in pathophysiological tissue samples often relies on immunohistochemical or immunofluorescence analysis. These techniques show target microanatomy by visualizing marker molecules on cells and their microenvironment. Here, we describe the "pros and cons" in each method, along with alternative procedures and the suggested imaging equipment.
authors: Center of Comparative Medicine, University of California, Davis, CA, USA.
authors: Hidetoshi
authors: H
authors: Mori
authors: Center of Comparative Medicine, University of California, Davis, CA, USA. rdcardiff@ucdavis.edu.
authors: Robert D
authors: RD
authors: Cardiff
mesh_terms: D000818:Animals; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*
keywords: Autofluorescence; Formalin-fixed paraffin-embedded tissues; Immunofluorescence; Immunohistochemistry; Zinc fixation