title: Nuclear Pore Permeabilization Is a Convergent Signaling Event in Effector-Triggered Immunity.
abstract: Nuclear transport of immune receptors, signal transducers, and transcription factors is an essential regulatory mechanism for immune activation. Whether and how this process is regulated at the level of the nuclear pore complex (NPC) remains unclear. Here, we report that CPR5, which plays a key inhibitory role in effector-triggered immunity (ETI) and programmed cell death (PCD) in plants, is a novel transmembrane nucleoporin. CPR5 associates with anchors of the NPC selective barrier to constrain nuclear access of signaling cargos and sequesters cyclin-dependent kinase inhibitors (CKIs) involved in ETI signal transduction. Upon activation by immunoreceptors, CPR5 undergoes an oligomer to monomer conformational switch, which coordinates CKI release for ETI signaling and reconfigures the selective barrier to allow significant influx of nuclear signaling cargos through the NPC. Consequently, these coordinated NPC actions result in simultaneous activation of diverse stress-related signaling pathways and constitute an essential regulatory mechanism specific for ETI/PCD induction.
authors: Department of Biology, Howard Hughes Medical Institute-Gordon and Betty Moore Foundation, P.O. Box 90338, Duke University, Durham, NC 27708, USA.
authors: Yangnan
authors: Y
authors: Gu
authors: Department of Biology, Howard Hughes Medical Institute-Gordon and Betty Moore Foundation, P.O. Box 90338, Duke University, Durham, NC 27708, USA.
authors: Sophia G
authors: SG
authors: Zebell
authors: School of Life Sciences, Center for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China.
authors: Zizhen
authors: Z
authors: Liang
authors: Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China.
authors: Shui
authors: S
authors: Wang
authors: School of Life Sciences, Center for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China.
authors: Byung-Ho
authors: BH
authors: Kang
authors: Department of Biology, Howard Hughes Medical Institute-Gordon and Betty Moore Foundation, P.O. Box 90338, Duke University, Durham, NC 27708, USA. Electronic address: xdong@duke.edu.
authors: Xinnian
authors: X
authors: Dong
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000276:immunology*; D017360:Arabidopsis / Q000235:genetics / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D022022:Nuclear Pore / Q000276:immunology*; D011487:Protein Conformation; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; C528008:CPR5 protein, Arabidopsis; D018797:Cell Cycle Proteins; D008565:Membrane Proteins