title: Analyzing the Tumor Microenvironment by Flow Cytometry.
abstract: Flow cytometry is an essential tool for studying the tumor microenvironment. It allows us to quickly quantify and identify multiple cell types in a heterogeneous sample. A brief overview of flow cytometry instrumentation and the appropriate considerations and steps in building a good flow cytometry staining panel are discussed. In addition, a lymphoid tissue and solid tumor leukocyte infiltrate flow cytometry staining protocol and an example of flow cytometry data analysis are presented.
authors: Lady Davis Institute for Medical Research, McGill University, Montreal, QC, Canada.
authors: Yoon Kow
authors: YK
authors: Young
authors: Lady Davis Institute for Medical Research, McGill University, Montreal, QC, Canada.
authors: Alicia M
authors: AM
authors: Bolt
authors: Lady Davis Institute for Medical Research, McGill University, Montreal, QC, Canada.
authors: Ryuhjin
authors: R
authors: Ahn
authors: Lady Davis Institute for Medical Research, McGill University, Montreal, QC, Canada. koren.mann@mcgill.ca.
authors: Koren K
authors: KK
authors: Mann
mesh_terms: D015415:Biomarkers; D005434:Flow Cytometry / Q000379:methods; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D016130:Immunophenotyping; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D059016:Tumor Microenvironment
chemical_list: D015415:Biomarkers
keywords: Compensation; Flow cytometry (FCM); Fluorescence minus one (FMO); Fluorescence-activated cell sorting (FACS); Immune cell infiltrate; Intracellular staining; Isotype control; Multi-parameter; Solid tumor; Spillover; Staining panel; Tumor microenvironment