title: A Single Mutation at Position 190 in Hemagglutinin Enhances Binding Affinity for Human Type Sialic Acid Receptor and Replication of H9N2 Avian Influenza Virus in Mice.
abstract: H9N2 avian influenza virus (AIV) has an extended host range, but the molecular basis underlying H9N2 AIV transmission to mammals remains unclear. We isolated more than 900 H9N2 AIVs in our 3-year surveillance in live bird markets in China from 2009 to 2012. Thirty-seven representative isolates were selected for further detailed characterization. These isolates were categorized into 8 genotypes (B64 to B71) and formed a distinct antigenic subgroup. Three isolates belonging to genotype B69, which is a predominant genotype circulating in China, replicated efficiently in mice, while the viruses tested in parallel in other genotypes replicated poorly, although they, like the three B69 isolates, have a leucine at position 226 in the hemagglutinin (HA) receptor binding site, which is critical for binding human type sialic acid receptors. Further molecular and single mutation analysis revealed that a valine (V) residue at position 190 in HA is responsible for efficient replication of these H9N2 viruses in mice. The 190V in HA does not affect virus receptor binding specificity but enhances binding affinity to human cells and lung tissues from mouse and humans. All these data indicate that the 190V in HA is one of the important determinants for H9N2 AIVs to cross the species barrier to infect mammals despite multiple genes conferring adaptation and replication of H9N2 viruses in mammals. Our findings provide novel insights on understanding host range expansion of H9N2 AIVs.
  
  
  OBJECTIVE
  Influenza virus hemagglutinin (HA) is responsible for binding to host cell receptors and therefore influences the viral host range and pathogenicity in different species. We showed that the H9N2 avian influenza viruses harboring 190V in the HA exhibit enhanced virus replication in mice. Further studies demonstrate that 190V in the HA does not change virus receptor binding specificity but enhances virus binding affinity of the H9N2 virus to human cells and attachment to lung tissues from humans and mouse. Our findings suggest that more attention should be given to the H9N2 AIVs with HA-190V during surveillance due to their potential threat to mammals, including humans.
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
authors: Qiaoyang
authors: Q
authors: Teng
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
authors: Dawei
authors: D
authors: Xu
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
authors: Weixia
authors: W
authors: Shen
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
authors: Qinfang
authors: Q
authors: Liu
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
authors: Guangyu
authors: G
authors: Rong
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
authors: Xuesong
authors: X
authors: Li
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
authors: Liping
authors: L
authors: Yan
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA.
authors: Jianmei
authors: J
authors: Yang
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
authors: Hongjun
authors: H
authors: Chen
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
authors: Hai
authors: H
authors: Yu
authors: Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, Kansas, USA wma@vet.k-state.edu lizejun@shvri.ac.cn.
authors: Wenjun
authors: W
authors: Ma
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China wma@vet.k-state.edu lizejun@shvri.ac.cn.
authors: Zejun
authors: Z
authors: Li
mesh_terms: D000072283:A549 Cells; D000818:Animals; D001717:Birds; D045744:Cell Line, Tumor; D004261:DNA Replication / Q000235:genetics; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics*; D005585:Influenza in Birds / Q000378:metabolism / Q000821:virology; D007251:Influenza, Human / Q000378:metabolism / Q000821:virology; D008168:Lung / Q000378:metabolism / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D009976:Orthomyxoviridae Infections / Q000378:metabolism / Q000821:virology; D010802:Phylogeny; D011956:Receptors, Cell Surface / Q000378:metabolism*; D053585:Virus Attachment; D014779:Virus Replication / Q000235:genetics*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011956:Receptors, Cell Surface; C092488:sialic acid receptor