title: Effect of Endomorphins on HUVECs Treated by ox-LDL and Its Related Mechanisms.
abstract: We found in the present study that treatment with ox-LDL decreased the cell viability and the content of nitric oxide (NO) and the activity of nitric oxide synthase (NOS) as well as eNOS mRNA expression, while increasing the mRNA expression and content of endothelin-1 (ET-1) in human umbilical vein endothelial cells (HUVECs). However, endomorphins EM1/EM2 increased the cell viability and the content of NO and the activity of NOS as well as eNOS mRNA expression, while decreasing the mRNA expression and content of ET-1 compared with ox-LDL alone. Meanwhile, the expressions of JNK and p-JNK were enhanced by ox-LDL while being suppressed by EM1/EM2. The results suggested that EM1 and EM2 can correct the endothelial cell dysfunction induced by ox-LDL and the protective effect may be achieved by affecting the JNK pathway.
authors: Department of Endocrinology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, China.
authors: Juan
authors: J
authors: Zhao
authors: Department of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, China.
authors: Qi
authors: Q
authors: Zhang
authors: Department of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, China.
authors: Jing
authors: 0000-0003-0862-0573
authors: J
authors: Liu
authors: Department of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, China.
authors: Liming
authors: L
authors: Tian
authors: Department of Endocrinology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, China.
authors: Wenhui
authors: W
authors: Huang
authors: Department of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, China.
authors: Jinxing
authors: J
authors: Quan
authors: Department of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, China.
authors: Jinyang
authors: J
authors: Wang
authors: Department of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, China.
authors: Yanjia
authors: Y
authors: Xu
authors: Department of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, China.
authors: Yunfang
authors: Y
authors: Wang
authors: Department of Nephrology, Gansu Provincial Hospital, 204 West Donggang Road, Lanzhou, Gansu 730000, China.
authors: Ruilan
authors: R
authors: Niu
mesh_terms: D002470:Cell Survival / Q000187:drug effects*; D019332:Endothelin-1 / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D019332:Endothelin-1; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase