title: Effects of concanavalin A on the progesterone production by bovine steroidogenic luteal cells in vitro.
abstract: The aim of this study was to evaluate the effects of concanavalin A (CONA) on the progesterone (P4) production by bovine steroidogenic luteal cells (LCs) in vitro. Luteal cells were collected during the mid-luteal stage (at 10-12 days following ovulation) and processed in the laboratory. Luteal cells were grown for 7 days in a humid atmosphere with 5% CO2 , with or without 10% foetal bovine serum, and were subjected to the following treatments: control: no treatment; CONA (10 μg/ml); LH (100 μg/ml); CONA + LH; LH (100 μg/ml) + prostaglandin F2α (PGF2α) (10 ng/ml); CONA + LH + PGF2α. Samples of the culture media were collected on days 1 (D1) and 7 (D7) for P4 quantification. The cells were counted on D7 of culture. Differences between treatments were considered statistically significant at p < .05. Culture in the presence of CONA decreased the P4-secreting capacity of LCs on D7 of culture, particularly in the absence of serum. The cell numbers did not change between treatments.
authors: Department of Animal Reproduction and Veterinary Radiology (Departamento de Reprodução Animal e Radiologia Veterinária), Faculdade de Medicina Veterinária e Zootecnia (FMVZ) - Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Botucatu, São Paulo, Brazil.
authors: F C
authors: FC
authors: Destro
authors: University of Uberaba (Universidade de Uberaba - Uniube), Uberaba, Minas Gerais, Brazil.
authors: I
authors: I
authors: Martin
authors: Department of Animal Reproduction and Veterinary Radiology (Departamento de Reprodução Animal e Radiologia Veterinária), Faculdade de Medicina Veterinária e Zootecnia (FMVZ) - Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Botucatu, São Paulo, Brazil.
authors: Fdc
authors: F
authors: Landim-Alvarenga
authors: Department of Animal Reproduction and Veterinary Radiology (Departamento de Reprodução Animal e Radiologia Veterinária), Faculdade de Medicina Veterinária e Zootecnia (FMVZ) - Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Botucatu, São Paulo, Brazil. jcpinheiroferreira@gmail.com.
authors: Jcp
authors: J
authors: Ferreira
authors: Department of Animal Science, Penn State University, University Park, PA, USA.
authors: J L
authors: JL
authors: Pate
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003208:Concanavalin A / Q000008:administration & dosage / Q000494:pharmacology*; D015237:Dinoprost / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D008184:Luteal Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000008:administration & dosage / Q000494:pharmacology; D011374:Progesterone / Q000378:metabolism*
chemical_list: D003208:Concanavalin A; D011374:Progesterone; D007986:Luteinizing Hormone; D015237:Dinoprost