title: "Bio-switch Chip" Based on Nanostructured Conducting Polymer and Entrapped Enzyme.
abstract: We report a switchable biochip strategy where enzymes were entrapped in conducting polymer layers and the enzymatic reaction of the entrapped enzymes was controlled in real-time via electrical stimuli on the polymer layers. This device is named here as a "bio-switch chip" (BSC). We fabricated BSC structures using polypyrrole (Ppy) with entrapped glucose oxidase (GOx) and demonstrated the switching of glucose oxidation reaction in real-time. We found that the introduction of a negative bias voltage on the BSC structure resulted in the enhanced glucose oxidation reaction by more than 20 times than that without a bias voltage. Moreover, because the BSC structures could be fabricated on specific regions, we could control the enzymatic reaction on specific regions. In view of the fact that enzymes enable very useful and versatile biochemical reactions, the ability to control the enzymatic reactions via conventional electrical signals could open up various applications in the area of biochips and other biochemical industries.
authors: Daesan
authors: D
authors: Kim
authors: Haneul
authors: H
authors: Yoo
authors: Department of Automotive Engineering, Doowon Technical University College , Anseong 456-718, Korea.
authors: Jae Yeol
authors: JY
authors: Park
authors: Seunghun
authors: S
authors: Hong
mesh_terms: D004800:Enzymes, Immobilized; D005947:Glucose; D005949:Glucose Oxidase; D049329:Nanostructures; D011108:Polymers; D011758:Pyrroles
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D011758:Pyrroles; D005949:Glucose Oxidase; D005947:Glucose
keywords: conducting polymer; controlling enzymatic activity; enzymatic reaction; glucose oxidase; polypyrrole