title: Polarized macrophages treated with nonylphenol differently regulate lipopolysaccharide-induced sepsis.
abstract: Nonylphenol (NP) as well-known "endocrine disrupter" influences sexual and reproductive development. Here, we investigated the effect of NP on M1-/M2-type macrophages and their role in lipopolysaccharide (LPS)-induced sepsis. Polarized macrophages of M1- and M2-types were obtained by the treatment with LPS and interleukin-4 (IL-4) to bone marrow-derived macrophages (BMDM), respectively. Coincubation of M1-macrophages with NP decreased COX-2, iNOS, IL-6, and TNF-α expression but no changes were detected in the production of nitric oxide (NO). Survival probability of LPS-induced sepsis mice was enhanced by the injection of NP-treated BMDM as compared to the injection of NP-untreated control BMDM. In the meanwhile, the expression of arginase 1(Arg1), a marker for M2-polarized macrophages was increased by the stimulation with LPS in BMDM. Arg1 expression was also enhanced by the treatment with IL-4 in BMDM, which was reduced by the coincubation with NP. Survival probability of LPS-induced sepsis mice was decreased by the injection of BMDM treated with IL-4 and NP as compared to the injection of IL-4-treated BMDM. It suggests that NP might inhibit macrophage function and the polarization to M2-macrophages. Taken together, data demonstrate that NP could differently affect immune responses of polarized macrophages resulted in the modulation of LPS-induced sepsis. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 2081-2089, 2016.
authors: Department of Bioscience and Biotechnology, Sejong University, Seoul, 05006, Republic of Korea.
authors: Jae-Wook
authors: JW
authors: Lee
authors: Department of Bioscience and Biotechnology, Sejong University, Seoul, 05006, Republic of Korea.
authors: Sojin
authors: S
authors: Park
authors: Department of Bioscience and Biotechnology, Sejong University, Seoul, 05006, Republic of Korea.
authors: Hye-Kyoung
authors: HK
authors: Han
authors: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Gyeonggi-do, 16419, Republic of Korea.|Department of Health Sciences and Technology, SAIHST, Samsung Medical Center, Sungkyunkwan University, Seoul, 06351, Republic of Korea.
authors: Sung Hee
authors: SH
authors: Um
authors: Department of Bioscience and Biotechnology, Sejong University, Seoul, 05006, Republic of Korea.
authors: Eun-Yi
authors: EY
authors: Moon
mesh_terms: D000818:Animals; D001119:Arginase / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D016764:Cell Polarity; D051546:Cyclooxygenase 2 / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity*; D015847:Interleukin-4 / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010636:Phenols / Q000633:toxicity*; D018805:Sepsis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010636:Phenols; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D009569:Nitric Oxide; C025256:nonylphenol; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; C544448:Arg1 protein, mouse; D001119:Arginase
keywords: LPS-induced sepsis; M1-/M2-polarized macrophages; endocrine disruptor; nonylphenol