title: Comparative analysis of L-sorbose dehydrogenase by docking strategy for 2-keto-L-gulonic acid production in Ketogulonicigenium vulgare and Bacillus endophyticus consortium.
abstract: Improving the yield of 2-keto-L-gulonic acid (2-KGA), the direct precursor of vitamin C, draws more and more attention in industrial production. In this study, we try to increase the 2-KGA productivity by computer-aided selection of genes encoding L-sorbose dehydrogenases (SDH) of Ketogulonicigenium vulgare. First, six SDHs were modeled by docking strategy to predict the binding mode with co-factor PQQ. The binding energy between SSDA1-H/SSDA1-L and PQQ was the highest, followed by SSDA3/SSDA2. The binding energy between SSDA1-P/SSDB and PQQ was the lowest. Then, these genes were overexpressed, respectively, in an industrial strain K. vulgare HKv604. Overexpression of ssda1-l and ssda1-h enhanced the 2-KGA production by 7.89 and 12.56 % in mono-cultured K. vulgare, and by 13.21 and 16.86 % when K. vulgare was co-cultured with Bacillus endophyticus. When the engineered K. vulgare SyBE_Kv000116013 (overexpression of ssda1-p) or SyBE_Kv000116016 (overexpression of ssdb) was co-cultured with B. endophyticus, the 2-KGA production decreased significantly. The docking results were in accordance with the experimental data, which indicated that computer-aided modeling is an efficient strategy for screening more efficient enzymes.
authors: Key Laboratory of Systems Bioengineering (Ministry of Education) School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China.
authors: Si
authors: S
authors: Chen
authors: Key Laboratory of Systems Bioengineering (Ministry of Education) School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China.
authors: Nan
authors: N
authors: Jia
authors: Key Laboratory of Systems Bioengineering (Ministry of Education) School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China. mzding@tju.edu.cn.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China. mzding@tju.edu.cn.
authors: Ming-Zhu
authors: MZ
authors: Ding
authors: Key Laboratory of Systems Bioengineering (Ministry of Education) School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China.
authors: Ying-Jin
authors: YJ
authors: Yuan
mesh_terms: D001407:Bacillus / Q000502:physiology*; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018920:Coculture Techniques; D005285:Fermentation; D060847:Metabolic Engineering; D059013:Microbial Consortia; D062105:Molecular Docking Simulation; D045542:PQQ Cofactor / Q000737:chemistry; D041902:Rhodobacteraceae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D013400:Sugar Acids / Q000378:metabolism*
chemical_list: D013400:Sugar Acids; D045542:PQQ Cofactor; D002237:Carbohydrate Dehydrogenases; C104557:sorbose dehydrogenase; C011491:provitamin C
keywords: 2-keto-L-gulonic acid; Bacillus endophyticus; Ketogulonicigenium vulgare; Microbial consortium