title: Characterization of a Novel IncHI2 Plasmid Carrying Tandem Copies of blaCTX-M-2 in a fosA6-Harboring Escherichia coli Sequence Type 410 Strain.
abstract: The extended-spectrum β-lactamase gene blaCTX-M-2is mainly associated with ISCR1 embedded in complex sul1-type integrons, but information on the genetic context of plasmids harboring the ISCR1-blaCTX-M-2module remains limited. In this study, a blaCTX-M-2-harboring plasmid (pYD786-1) belonging to the sequence type 2 (ST2)-IncHI2 plasmid type and isolated from an Escherichia coli ST410 clinical strain was sequenced and analyzed. pYD786-1 belongs to the APEC-O1-R-type IncHI2 plasmids, which are widely distributed in human, poultry, and livestock strains. It contains a multidrug resistance mosaic region (MRR) consisting of a Tn21::In2 transposon backbone augmented by acquisition of duplicate ISCR1-blaCTX-M-2modules. Tn2411, a Tn21::In2 precursor, likely played a role in the generation of the MRR in pN13-01290_23, the putative progenitor plasmid of pYD786-1, found in a foodborne Salmonella strain. Tn21/Tn2411::In::ISCR1-blaCTX-M-2derivatives, including pYD786-1, have been identified in strains from Europe, South America, and the United States, suggesting potential global dissemination of the blaCTX-M-2modules mediated by this vehicle.
authors: Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.|Division of Infectious Diseases, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors: Qinglan
authors: Q
authors: Guo
authors: Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.
authors: Baixing
authors: B
authors: Ding
authors: University of Limoges, INSERM, CHU Limoges, UMR_S 1092, Limoges, France.
authors: Thomas
authors: T
authors: Jové
authors: Modernizing Medical Microbiology Consortium, Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom.
authors: Nicole
authors: 0000-0002-4508-7969
authors: N
authors: Stoesser
authors: Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
authors: Vaughn S
authors: 0000-0001-7726-0765
authors: VS
authors: Cooper
authors: Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.
authors: Minggui
authors: M
authors: Wang
authors: Division of Infectious Diseases, University of Pittsburgh, Pittsburgh, Pennsylvania, USA yod4@pitt.edu.
authors: Yohei
authors: Y
authors: Doi
mesh_terms: D000328:Adult; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004251:DNA Transposable Elements; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology / Q000635:transmission; D005060:Europe; D005260:Female; D005578:Fosfomycin / Q000494:pharmacology; D015870:Gene Expression; D022761:Gene Transfer, Horizontal; D006723:Hong Kong; D006801:Humans; D032023:Integrons; D010802:Phylogeny; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013020:South America; D014481:United States; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D005578:Fosfomycin; C071167:beta-lactamase CTX-2; D001618:beta-Lactamases