title: Visualizing the Tumor Microenvironment of Liver Metastasis by Spinning Disk Confocal Microscopy.
abstract: Intravital microscopy has evolved into an invaluable technique to study the complexity of tumors by visualizing individual cells in live organisms. Here, we describe a method for employing intravital spinning disk confocal microscopy to picture high-resolution tumor-stroma interactions in real time. We depict in detail the surgical procedures to image various tumor microenvironments and different cellular components in the liver.
authors: Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada.
authors: Liane
authors: L
authors: Babes
authors: Calvin, Phoebe & Joan Snyder Institute for Chronic Diseases, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. pkubes@ucalgary.ca.
authors: Paul
authors: P
authors: Kubes
mesh_terms: D000818:Animals; D002450:Cell Communication; D004195:Disease Models, Animal; D000069416:Intravital Microscopy / Q000295:instrumentation / Q000379:methods; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000556:secondary*; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D059016:Tumor Microenvironment
keywords: Imaging; Intravital imaging; Liver; Metastasis; Mouse models; Spinning disk confocal microscopy; Transgenic mice; Tumor microenvironment