title: Chemotactic Activity of Cyclophilin A in the Skin Mucus of Yellow Catfish (Pelteobagrus fulvidraco) and Its Active Site for Chemotaxis.
abstract: Fish skin mucus is a dynamic barrier for invading pathogens with a variety of anti-microbial enzymes, including cyclophilin A (CypA), a multi-functional protein with peptidyl-prolyl cis/trans isomerase (PPIase) activity. Beside various other immunological functions, CypA induces leucocytes migration in vitro in teleost. In the current study, we have discovered several novel immune-relevant proteins in yellow catfish skin mucus by mass spectrometry (MS). The CypA present among them was further detected by Western blot. Moreover, the CypA present in the skin mucus displayed strong chemotactic activity for yellow catfish leucocytes. Interestingly, asparagine (like arginine in mammals) at position 69 was the critical site in yellow catfish CypA involved in leucocyte attraction. These novel efforts do not only highlight the enzymatic texture of skin mucus, but signify CypA to be targeted for anti-inflammatory therapeutics.
authors: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. farmandawar2012@yahoo.com.
authors: Farman Ullah
authors: FU
authors: Dawar
authors: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. tujiagang@mail.hzau.edu.cn.
authors: Jiagang
authors: J
authors: Tu
authors: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. 13697134024@163.com.
authors: Yang
authors: Y
authors: Xiong
authors: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. lanjiangfeng@mail.hzau.edu.cn.
authors: Jiangfeng
authors: J
authors: Lan
authors: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. dongxingxinghg@163.com.
authors: Xing Xing
authors: XX
authors: Dong
authors: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. liuxl@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. liuxl@mail.hzau.edu.cn.
authors: Xiaoling
authors: X
authors: Liu
authors: Department of Zoology, Hazara University, Mansehra 21300, Pakistan. mnasir43663@googlemail.com.
authors: Muhammad Nasir Khan
authors: MN
authors: Khattak
authors: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. jmei@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. jmei@mail.hzau.edu.cn.
authors: Jie
authors: J
authors: Mei
authors: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. linli@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. linli@mail.hzau.edu.cn.
authors: Li
authors: L
authors: Lin
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D002397:Catfishes / Q000378:metabolism* / Q000502:physiology; D002633:Chemotaxis; D021984:Cyclophilin A / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007962:Leukocytes / Q000502:physiology; D009093:Mucus / Q000378:metabolism; D012867:Skin / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D021984:Cyclophilin A
keywords: chemotaxis; cyclophilin A; immune response; skin mucus; yellow catfish