title: Expression and Significance of RANTES and MCP-1 in Renal Tissue With Chronic Renal Allograft Dysfunction.
abstract: BACKGROUND
  To investigate the expression of RANTES (regulated upon activation, normal T-cell-expressed and -secreted) and monocyte chemoattractant protein-1 (MCP-1) in renal allografts with chronic renal allograft dysfunction (CRAD), and explore its relationship with interstitial fibrosis and tubular atrophy (IF/TA).
  
  
  METHODS
  An immunohistochemical assay and computer-assisted, genuine colored image analysis system were used to detect the expression of RANTES and MCP-1 in renal allografts with CRAD. The relationship among the expression level of MCP-1, RANTES, and the grade of inflammatory cell infiltration, interstitial fibrosis, and tubular atrophy in renal allografts were analyzed. Ten specimens of healthy renal tissue were used as controls.
  
  
  RESULTS
  Compared to the normal tissues, the expressions of RANTES and MCP-1 were significantly higher in the renal tissues with CRAD (P < .001), and the expressions tended to increase along with the pathological grade of IF/TA. The expression of RANTES and MCP-1 were positively correlated with the pathological grades of IF/TA (r = 0.940 and 0.954 respectively, P < .001 for both).
  
  
  CONCLUSIONS
  In renal allograft tissue with CRAD, the up-regulated expressions of RANTES and MCP-1 may be related to the progression of chronic renal allograft dysfunction and allograft fibrosis.
authors: Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guangxi, China. Electronic address: yanqiang1967@sohu.com.
authors: Q
authors: Q
authors: Yan
authors: Department of Urology, The Fifth Affiliated Hospital of Zunyi Medical College, Zhuhai, China.
authors: H
authors: H
authors: Jiang
authors: Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guangxi, China.
authors: B
authors: B
authors: Wang
authors: Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guangxi, China.
authors: W
authors: W
authors: Sui
authors: Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guangxi, China.
authors: H
authors: H
authors: Zhou
authors: Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guangxi, China.
authors: G
authors: G
authors: Zou
mesh_terms: D018932:Chemokine CCL2 / Q000097:blood* / Q000378:metabolism; D018946:Chemokine CCL5 / Q000097:blood* / Q000378:metabolism; D005355:Fibrosis; D006801:Humans; D016030:Kidney Transplantation; D051436:Renal Insufficiency, Chronic / Q000097:blood* / Q000209:etiology* / Q000473:pathology
chemical_list: C111835:CCL2 protein, human; C483024:CCL5 protein, human; D018932:Chemokine CCL2; D018946:Chemokine CCL5