title: Titania nanotubes decorated with gold nanoparticles for electrochemiluminescent biosensing of glycosylated hemoglobin.
abstract: A glycated hemoglobin (HbA1c) biosensor with high performance has been constructed in this work. Here the fructosyl amino acid oxidase was immobilized onto a pre-functionalized indium tin oxide glass with titania nanotubes decorated with gold nanoparticles. The property of nanocomposite was characterized by transmission electromicroscopy, scanning electron microscopy, electrochemistry and spectroscopy. Under the optimum conditions, fructosyl valine was detected by this biosensor. It exhibited a linear detection range from 4.0 × 10(-9) M to 7.2 × 10(-7) M, and a limit of detection for 3.8 × 10(-9) M at the signal-to-noise ratio of 3. Thus the HbA1c level in whole blood samples of healthy individuals or diabetic patients were evaluated with designed biosensor after pre-treatment of hydrolysis. The results of our detection were closely consistent with that of the standard method. At the same time, our biosensor has some advantages including high sensitivity, disposable usage and low cost, which implies its great promising application in point-of-care testing of HbA1c.
authors: Institute of Analytical Chemistry, Dushu Lake Campus, Soochow University, Industrial Park, Suzhou, 215123, PR China; College of Chemistry and Chemical Engineering, Nantong University, Nantong, 226019, PR China.
authors: Qun
authors: Q
authors: Zhao
authors: Institute of Analytical Chemistry, Dushu Lake Campus, Soochow University, Industrial Park, Suzhou, 215123, PR China.
authors: Shan
authors: S
authors: Tang
authors: Institute of Analytical Chemistry, Dushu Lake Campus, Soochow University, Industrial Park, Suzhou, 215123, PR China; Second Affiliated Hospital, Soochow University, Suzhou, 215004, PR China.
authors: Chen
authors: C
authors: Fang
authors: Institute of Analytical Chemistry, Dushu Lake Campus, Soochow University, Industrial Park, Suzhou, 215123, PR China. Electronic address: tuyf@suda.edu.cn.
authors: Yi-Feng
authors: YF
authors: Tu
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000378:metabolism; D006031:Glycosylation; D006046:Gold / Q000737:chemistry / Q000378:metabolism; D006454:Hemoglobins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008163:Luminescent Measurements; D053768:Metal Nanoparticles / Q000737:chemistry; D043942:Nanotubes / Q000737:chemistry; D014025:Titanium / Q000737:chemistry / Q000378:metabolism
chemical_list: D000594:Amino Acid Oxidoreductases; D006442:Glycated Hemoglobin; D006046:Gold; D006454:Hemoglobins; D014025:Titanium; C517652:hemoglobin A1c protein, human; C009495:titanium dioxide; C103012:amadoriase
keywords: AuNPs/TiNTs; Electrochemiluminescence; Fructosyl amino-acid oxidase; Glycosylated hemoglobin; Luminol