title: Single-Cell Cytokine Profiling to Investigate Cellular Functional Diversity in Hematopoietic Malignancies.
abstract: Single-cell analysis of cytokine production is increasingly recognized as an important method to understand the inflammatory microenvironment and hematopoietic disease state. Certain cytokines are critical to the regulation of lineage specification, and the aberrant production of these cytokines can contribute to lineage reprogramming. Here, we describe of a platform combining subnanoliter microchambers and a high-density antibody barcode array for the study of single-cell cytokine secretions in hematopoietic cancer cell populations.
authors: Department of Biomedical Engineering, Yale University, PO Box 208260, New Haven, CT, 06520, USA.
authors: Jonathan J
authors: JJ
authors: Chen
authors: Department of Biomedical Engineering, Yale University, PO Box 208260, New Haven, CT, 06520, USA.
authors: Minsuk
authors: M
authors: Kwak
authors: Department of Biomedical Engineering, Yale University, PO Box 208260, New Haven, CT, 06520, USA. rong.fan@yale.edu.
authors: Rong
authors: R
authors: Fan
mesh_terms: D016207:Cytokines / Q000378:metabolism*; D005434:Flow Cytometry; D019337:Hematologic Neoplasms / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D059010:Single-Cell Analysis / Q000379:methods*; D046888:Tissue Array Analysis
chemical_list: D016207:Cytokines
keywords: Cytokine secretions; Functional heterogeneity; High-throughput multiplexed analysis; Immunophenotyping; Pathogenesis; Single-cell cytokine profiling