title: Mutations associated with familial Parkinson's disease alter the initiation and amplification steps of α-synuclein aggregation.
abstract: Parkinson's disease is a highly debilitating neurodegenerative condition whose pathological hallmark is the presence in nerve cells of proteinacious deposits, known as Lewy bodies, composed primarily of amyloid fibrils of α-synuclein. Several missense mutations in the gene encoding α-synuclein have been associated with familial variants of Parkinson's disease and have been shown to affect the kinetics of the aggregation of the protein. Using a combination of experimental and theoretical approaches, we present a systematic in vitro study of the influence of disease-associated single-point mutations on the individual processes involved in α-synuclein aggregation into amyloid fibrils. We find that lipid-induced fibril production and surface catalyzed fibril amplification are the processes most strongly affected by these mutations and show that familial mutations can induce dramatic changes in the crucial processes thought to be associated with the initiation and spreading of the aggregation of α-synuclein.
authors: Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom.
authors: Patrick
authors: 0000-0002-1204-5340
authors: P
authors: Flagmeier
authors: Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom.
authors: Georg
authors: 0000-0002-6562-7715
authors: G
authors: Meisl
authors: Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom.
authors: Michele
authors: M
authors: Vendruscolo
authors: Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom.
authors: Tuomas P J
authors: TP
authors: Knowles
authors: Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom cmd44@cam.ac.uk alexander.buell@uni-duesseldorf.de cg393@cam.ac.uk.
authors: Christopher M
authors: CM
authors: Dobson
authors: Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom cmd44@cam.ac.uk alexander.buell@uni-duesseldorf.de cg393@cam.ac.uk.
authors: Alexander K
authors: AK
authors: Buell
authors: Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom cmd44@cam.ac.uk alexander.buell@uni-duesseldorf.de cg393@cam.ac.uk.
authors: Céline
authors: 0000-0001-9753-3310
authors: C
authors: Galvagnion
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007700:Kinetics; D008055:Lipids / Q000737:chemistry; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics; D010300:Parkinson Disease / Q000235:genetics* / Q000473:pathology; D066263:Protein Aggregation, Pathological / Q000235:genetics*; D051844:alpha-Synuclein / Q000737:chemistry / Q000235:genetics*
chemical_list: D000682:Amyloid; D008055:Lipids; D009419:Nerve Tissue Proteins; D051844:alpha-Synuclein
keywords: familial Parkinson’s disease; kinetic analysis; lipid-induced aggregation; neurodegenerative disease; seeded aggregation