title: Fell-Muir Lecture: Regulatory mechanisms of skeletal and connective tissue development and homeostasis - lessons from studies of human disorders.
abstract: Studies of proliferative hemangiomas have led to the discovery that interactions of endothelial cells with extracellular matrix and/or Vascular Endothelial Growth Factor (VEGF)-A stimulate the expression of VEGFR1, the VEGF decoy receptor, and suppress VEGF-dependent VEGFR2 signalling by a mechanism that requires the matrix-binding receptor Anthrax Toxin Receptor (ANTXR)1, VEGFR2, β1 integrin and the Nuclear Factor of Activated T cells (NFAT). In hemangioma endothelial cells, all these components are present, but are functionally compromised, so that the levels of VEGFR1 are extremely low and VEGFR2 signalling is constitutively active. Consequently, the levels of Hypoxia Inducible Factor (HIF)-1α and its transcriptional targets, VEGF-A and C-X-C motif chemokine 12 (CxCl12), are elevated and a positive VEGF-A feedback loop is established. Overexpression of ANTXR1, carrying a heterozygous Ala-to-Thr mutation, induces hemangioma-like signalling in control endothelial cells; VEGF signalling is normalized when wild-type ANTXR1 is overexpressed in hemangioma cells. These findings suggest that ANTXR1 functions as a negative regulator of VEGF-A signalling. Studies of a mouse model of the Growth Retardation, Alopecia, Pseudo-anodontia and Optic Atrophy (GAPO) syndrome, caused by the loss-of-function mutations in ANTXR1, as well as knock-in mice carrying the Ala-to-Thr ANTXR1 mutation, confirm that ANTXR1 functions as a suppressor of VEGF-A signalling. Cutaneous endothelial cells isolated from ANTXR1-deficient mice exhibit low levels of VEGFR1, elevated levels of VEGF-A, HIF-1α and CxCl12 and activated VEGFR2 signalling as in hemangioma. Increased numbers of myeloid cells in the skin of ANTXR1-deficient mice are associated with reduced vascularity and increased skin fibrosis, suggesting a mechanism for hemangioma involution and replacement by fibrotic scars. Through controlling VEGF-A signalling and extracellular matrix synthesis, ANTXR1 is emerging as a key regulator of skeletal and connective tissue development and homeostasis.
authors: Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA. bjorn_olsen@hms.harvard.edu.
authors: Bjorn R
authors: BR
authors: Olsen
authors: Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA.
authors: Agnes D
authors: AD
authors: Berendsen
authors: Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA.
authors: Tatiana Y
authors: TY
authors: Besschetnova
authors: Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA.
authors: Xuchen
authors: X
authors: Duan
authors: Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA.
authors: Kai
authors: K
authors: Hu
mesh_terms: D000818:Animals; D001846:Bone Development / Q000502:physiology*; D003238:Connective Tissue / Q000254:growth & development*; D006391:Hemangioma / Q000378:metabolism* / Q000473:pathology; D006706:Homeostasis / Q000502:physiology*; D006801:Humans; D008840:Microfilament Proteins; D009363:Neoplasm Proteins / Q000502:physiology; D011956:Receptors, Cell Surface / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
chemical_list: C473287:ANTXR1 protein, human; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C000588866:KDR protein, human; D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: GAPO syndrome; anthrax toxin receptor 1; hemangioma; paracrine and intracrine VEGF-A signalling; skeletal development; vascular endothelial growth factor