title: Surface passivity largely governs the bioaccessibility of nickel-based powder particles at human exposure conditions.
abstract: The European chemical framework REACH requires that hazards and risks posed by chemicals, including alloys and metals, are identified and proven safe for humans and the environment. Therefore, differences in bioaccessibility in terms of released metals in synthetic biological fluids (different pH (1.5-7.4) and composition) that are relevant for different human exposure routes (inhalation, ingestion, and dermal contact) have been assessed for powder particles of an alloy containing high levels of nickel (Inconel 718, 57 wt% nickel). This powder is compared with the bioaccessibility of two nickel-containing stainless steel powders (AISI 316L, 10-12% nickel) and with powders representing their main pure alloy constituents: two nickel metal powders (100% nickel), two iron metal powders and two chromium metal powders. X-ray photoelectron spectroscopy, microscopy, light scattering, and nitrogen absorption were employed for the particle and surface oxide characterization. Atomic absorption spectroscopy was used to quantify released amounts of metals in solution. Cytotoxicity (Alamar blue assay) and DNA damage (comet assay) of the Inconel powder were assessed following exposure of the human lung cell line A549, as well as its ability to generate reactive oxygen species (DCFH-DA assay). Despite its high nickel content, the Inconel alloy powder did not release any significant amounts of metals and did not induce any toxic response. It is concluded, that this is related to the high surface passivity of the Inconel powder governed by its chromium-rich surface oxide. Read-across from the pure metal constituents is hence not recommended either for this or any other passive alloy.
authors: KTH Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Division of Surface and Corrosion Science, Drottning Kristinas v. 51, SE-10044, Stockholm, Sweden; Unit of Work Environment Toxicology, Institute of Environmental Medicine, Karolinska Institutet, SE-171 77, Stockholm, Sweden. Electronic address: yolanda@kth.se.
authors: Yolanda S
authors: YS
authors: Hedberg
authors: KTH Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Division of Surface and Corrosion Science, Drottning Kristinas v. 51, SE-10044, Stockholm, Sweden.
authors: Gunilla
authors: G
authors: Herting
authors: Department of Environmental Science and Analytical Chemistry, Stockholm University, Svante Arrhenius v.8, SE-106 91, Stockholm, Sweden.
authors: Siiri
authors: S
authors: Latvala
authors: Department of Environmental Science and Analytical Chemistry, Stockholm University, Svante Arrhenius v.8, SE-106 91, Stockholm, Sweden.
authors: Karine
authors: K
authors: Elihn
authors: Unit of Biochemical Toxicology, Institute of Environmental Medicine, Karolinska Institutet, SE-171 77, Stockholm, Sweden.
authors: Hanna L
authors: HL
authors: Karlsson
authors: KTH Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Division of Surface and Corrosion Science, Drottning Kristinas v. 51, SE-10044, Stockholm, Sweden.
authors: Inger
authors: I
authors: Odnevall Wallinder
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002858:Chromium Alloys / Q000737:chemistry / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019570:Inhalation Exposure / Q000009:adverse effects; D008027:Light; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D009532:Nickel / Q000737:chemistry / Q000633:toxicity*; D056951:Photoelectron Spectroscopy; D011208:Powders; D017382:Reactive Oxygen Species / Q000737:chemistry; D018570:Risk Assessment; D053838:Scattering, Small Angle; D012995:Solubility; D013054:Spectrophotometry, Atomic; D013193:Stainless Steel / Q000737:chemistry / Q000633:toxicity; D013499:Surface Properties; D018675:Toxicity Tests / Q000379:methods
chemical_list: D002858:Chromium Alloys; D011208:Powders; D017382:Reactive Oxygen Species; D013193:Stainless Steel; D009532:Nickel
keywords: Alloy; Bioaccessibility; Inconel; Nickel; Toxicity