title: Decoding the principles underlying the frequency of association with nucleoli for RNA polymerase III-transcribed genes in budding yeast.
abstract: The association of RNA polymerase III (Pol III)-transcribed genes with nucleoli seems to be an evolutionarily conserved property of the spatial organization of eukaryotic genomes. However, recent studies of global chromosome architecture in budding yeast have challenged this view. We used live-cell imaging to determine the intranuclear positions of 13 Pol III-transcribed genes. The frequency of association with nucleolus and nuclear periphery depends on linear genomic distance from the tethering elements-centromeres or telomeres. Releasing the hold of the tethering elements by inactivating centromere attachment to the spindle pole body or changing the position of ribosomal DNA arrays resulted in the association of Pol III-transcribed genes with nucleoli. Conversely, ectopic insertion of a Pol III-transcribed gene in the vicinity of a centromere prevented its association with nucleolus. Pol III-dependent transcription was independent of the intranuclear position of the gene, but the nucleolar recruitment of Pol III-transcribed genes required active transcription. We conclude that the association of Pol III-transcribed genes with the nucleolus, when permitted by global chromosome architecture, provides nucleolar and/or nuclear peripheral anchoring points contributing locally to intranuclear chromosome organization.
authors: Laboratoire de Biologie Moléculaire Eucaryote, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 31000 Toulouse, France.
authors: Praveen
authors: P
authors: Belagal
authors: Laboratoire de Biologie Moléculaire Eucaryote, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 31000 Toulouse, France.
authors: Christophe
authors: C
authors: Normand
authors: Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Hyderabad 500007, India.
authors: Ashutosh
authors: A
authors: Shukla
authors: Laboratoire de Biologie Moléculaire Eucaryote, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 31000 Toulouse, France.
authors: Renjie
authors: R
authors: Wang
authors: Laboratoire de Biologie Moléculaire Eucaryote, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 31000 Toulouse, France.
authors: Isabelle
authors: I
authors: Léger-Silvestre
authors: Laboratoire de Biologie Moléculaire Eucaryote, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 31000 Toulouse, France.
authors: Christophe
authors: C
authors: Dez
authors: Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Hyderabad 500007, India purnima@ccmb.res.in gadal@biotoul.fr.
authors: Purnima
authors: P
authors: Bhargava
authors: Laboratoire de Biologie Moléculaire Eucaryote, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 31000 Toulouse, France purnima@ccmb.res.in gadal@biotoul.fr.
authors: Olivier
authors: O
authors: Gadal
mesh_terms: D002466:Cell Nucleolus / Q000378:metabolism* / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D002503:Centromere / Q000378:metabolism* / Q000502:physiology; D002875:Chromosomes / Q000502:physiology; D061848:Optical Imaging / Q000379:methods; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism; D012320:RNA Polymerase III / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D004718:Saccharomycetales / Q000235:genetics / Q000378:metabolism; D016615:Telomere / Q000502:physiology; D014158:Transcription, Genetic
chemical_list: D010454:Peptide Termination Factors; C080775:SUP45 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012320:RNA Polymerase III