title: Electric pulses: a flexible tool to manipulate cytosolic calcium concentrations and generate spontaneous-like calcium oscillations in mesenchymal stem cells.
abstract: Human adipose mesenchymal stem cells (haMSCs) are multipotent adult stem cells of great interest in regenerative medicine or oncology. They present spontaneous calcium oscillations related to cell cycle progression or differentiation but the correlation between these events is still unclear. Indeed, it is difficult to mimic haMSCs spontaneous calcium oscillations with chemical means. Pulsed electric fields (PEFs) can permeabilise plasma and/or organelles membranes depending on the applied pulses and therefore generate cytosolic calcium peaks by recruiting calcium from the external medium or from internal stores. We show that it is possible to mimic haMSCs spontaneous calcium oscillations (same amplitude, duration and shape) using 100 μs PEFs or 10 ns PEFs. We propose a model that explains the experimental situations reported. PEFs can therefore be a flexible tool to manipulate cytosolic calcium concentrations. This tool, that can be switched on and off instantaneously, contrary to chemicals agents, can be very useful to investigate the role of calcium oscillations in cell physiology and/or to manipulate cell fate.
authors: Marie-Amelie
authors: MA
authors: de Menorval
authors: Franck M
authors: FM
authors: Andre
authors: Aude
authors: A
authors: Silve
authors: Claire
authors: C
authors: Dalmay
authors: Olivier
authors: O
authors: Français
authors: Bruno
authors: B
authors: Le Pioufle
authors: Lluis M
authors: LM
authors: Mir
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D002118:Calcium / Q000378:metabolism / Q000528:radiation effects; D020013:Calcium Signaling / Q000235:genetics*; D002136:Calcium, Dietary; D002454:Cell Differentiation / Q000528:radiation effects*; D003600:Cytosol / Q000378:metabolism / Q000528:radiation effects; D004560:Electricity; D004574:Electromagnetic Fields; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000528:radiation effects*; D044968:Regenerative Medicine
chemical_list: D002136:Calcium, Dietary; D002118:Calcium