title: Validation Study on a Rapid Method for Simultaneous Determination of Pesticide Residues in Vegetables and Fruits by LC-MS/MS.
abstract: A validation study was carried out on a rapid method for the simultaneous determination of pesticide residues in vegetables and fruits by LC-MS/MS. Preparation of the test solution was performed by a solid-phase extraction technique with QuEChERS (STQ method). Pesticide residues were extracted with acetonitrile using a homogenizer, followed by salting-out and dehydration at the same time. The acetonitrile layer was purified with C18 and PSA mini-columns. The method was assessed for 130 pesticide residues in 14 kinds of vegetables and fruits at the concentration level of 0.01 μg/g according to the method validation guideline of the Ministry of Health, Labour and Welfare of Japan. As a result 75 to 120 pesticide residues were determined satisfactorily in the tested samples. Thus, this method could be useful for a rapid and simultaneous determination of multi-class pesticide residues in various vegetables and fruits.
authors: Osaka City Institute of Public Health and Environmental Sciences.
authors: Tamaki
authors: T
authors: Sato
authors: Iori
authors: I
authors: Miyamoto
authors: Masako
authors: M
authors: Uemura
authors: Tadashi
authors: T
authors: Nakatani
authors: Naoya
authors: N
authors: Kakutani
authors: Tetsuo
authors: T
authors: Yamano
mesh_terms: D000097:Acetonitriles; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D006077:Government Agencies; D017408:Guidelines as Topic; D007564:Japan; D010573:Pesticide Residues / Q000032:analysis* / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D000097:Acetonitriles; D010573:Pesticide Residues; C032159:acetonitrile