title: Marked elevation of serum M2BP-adiponectin complex in men with coronary artery disease.
abstract: Adiponectin (APN) is an adipocyte-derived bioactive molecule with anti-diabetic and anti-atherogenic properties. Although the anti-diabetic effects of APN are mediated by AdipoR1 and AdipoR2, the anti-atherogenic mechanisms of APN remain unclear. The aim of this study was to determine the serum molecule inhibiting APN functions.
  
  
  
  By immunoprecipitation with an anti-APN antibody and mass spectrometry, we identified Mac-2 binding protein (M2BP) as a novel serum APN-binding protein. The association of M2BP and APN was confirmed using reconstituted proteins in vitro. Serum M2BP-APN complex levels were markedly higher in male patients with coronary artery disease (CAD) than in healthy subjects. M2BP abrogated the suppressive effects of APN on tumour necrosis factor (TNF)-α-induced inflammation in vascular endothelial cells.
  
  
  
  The increment of serum M2BP-APN complex could be a novel risk factor for CAD, through the abolishment of the anti-atherogenic effects of APN.
authors: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Ryu
authors: R
authors: Niinaga
authors: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Hiroyasu
authors: H
authors: Yamamoto
authors: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Manami
authors: M
authors: Yoshii
authors: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Hiromi
authors: H
authors: Uekita
authors: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Noriko
authors: N
authors: Yamane
authors: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Ikoi
authors: I
authors: Kochi
authors: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Akane
authors: A
authors: Matsumoto
authors: Department of Cardiology, Hyogo Prefectural Nishinomiya Hospital, Hyogo, Japan.
authors: Tetsuro
authors: T
authors: Matsuoka
authors: Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan. Electronic address: skihara@sahs.med.osaka-u.ac.jp.
authors: Shinji
authors: S
authors: Kihara
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D052242:Adiponectin / Q000097:blood*; D000368:Aged; D000369:Aged, 80 and over; D060050:Angina, Stable / Q000097:blood; D000951:Antigens, Neoplasm / Q000097:blood*; D050197:Atherosclerosis / Q000097:blood; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood*; D042783:Endothelial Cells / Q000378:metabolism; D057809:HEK293 Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D047468:Immunoprecipitation; D007249:Inflammation; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood*; D008875:Middle Aged; D054419:Receptors, Adiponectin / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D012307:Risk Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D000951:Antigens, Neoplasm; D008562:Membrane Glycoproteins; D054419:Receptors, Adiponectin; D011994:Recombinant Proteins; C114782:TAA90K protein, human; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha
keywords: Adiponectin; Atherosclerosis; Coronary artery disease; Inflammation; Mac-2 binding protein