title: Cytotoxic cardiac glycosides from the fruit (pods) of Adenium obesum (Forssk.) Roem. & Schult.
abstract: Phytochemical investigation of methanolic extract of Adenium obesum led to the isolation of 42 (1-42) compounds belongs to cardiac glycosides, triterpenoids and steroids. The chemical structures of isolated compounds were elucidated by spectral techniques UV, IR, NMR and FAB MS. The cardiac glycosides were tested against three human cell lines, 3T3 (normal cells), HeLa (Human cervical cancer cell lines) and PC-3 (Human prostate cancer cell lines). The cardiac glycoside, honghelin (4), obeside B (5) and obeside C (6) showed significant effects against cell lines Hela, 3T3 and PC-3 compared to standard drug doxorubicin. Compounds 4, 5 and 6 exhibited very low IC50(μM) against the PC3 human cell line. 4 and 6 also showed least IC50against the HeLa human cell lines as compared to the standard drug doxorubicin whereas these three compounds showed effect on 3T3 cell line with high IC50values compared to drug cycloheximide.
authors: a Department of Chemistry , Federal Urdu University of Arts, Sciences and Technology , Karachi , Pakistan.
authors: Salman Khalid
authors: SK
authors: Ahmed
authors: a Department of Chemistry , Federal Urdu University of Arts, Sciences and Technology , Karachi , Pakistan.
authors: Muhammad Ali
authors: MA
authors: Versiani
authors: a Department of Chemistry , Federal Urdu University of Arts, Sciences and Technology , Karachi , Pakistan.
authors: Ambreen
authors: A
authors: Ikram
authors: a Department of Chemistry , Federal Urdu University of Arts, Sciences and Technology , Karachi , Pakistan.
authors: Samina Abdul
authors: SA
authors: Sattar
authors: b International Center for Chemical and Biological Sciences, HEJ Research Institute of Chemistry , University of Karachi , Karachi , Pakistan.
authors: Shaheen
authors: S
authors: Faizi
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D029062:Apocynaceae / Q000737:chemistry*; D002301:Cardiac Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D005638:Fruit / Q000737:chemistry; D006801:Humans; D010936:Plant Extracts / Q000032:analysis
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002301:Cardiac Glycosides; D010936:Plant Extracts
keywords: Adenium obesum; Apocynaceae; cancer cell lines; cardiac glycoside; cytotoxic; triterpenoids