title: Apoptosis as a Mechanism for Keratinocyte Death in Canine Toxic Epidermal Necrolysis.
abstract: In humans and dogs, toxic epidermal necrolysis (TEN) is a life-threatening dermatosis characterized by sudden epidermal death resulting in extensive skin detachment. There is little information on the pathogenesis of keratinocyte cell death in canine TEN. We studied the occurrence of apoptosis in skin lesions of dogs with TEN to determine if apoptosis contributes to the pathogenesis of this disease. Immunostaining with antibodies to activated caspase-3 and the terminal deoxynucleotidyl-transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick-end labeling technique revealed positive apoptotic keratinocytes in basal and suprabasal epidermal compartments in 17 biopsy specimens collected from 3 dogs with TEN and 16 from 3 dogs with erythema multiforme (EM). There was no significant difference in the number of positively stained epidermal cells between TEN and EM. These results suggest that apoptosis of epidermal keratinocytes and lymphocytic satellitosis represent one of the early steps in the pathogenesis of canine TEN, as in the human disease counterpart.
authors: 1 Department of Small Animal Medicine & Surgery, The University of Georgia, College of Veterinary Medicine, Athens, GA, USA.|2 Comparative Medicine Institute, NC State University, Raleigh, NC, USA.|3 Department of Clinical Sciences, NC State University, College of Veterinary Medicine, Raleigh, NC, USA.
authors: F
authors: F
authors: Banovic
authors: 3 Department of Clinical Sciences, NC State University, College of Veterinary Medicine, Raleigh, NC, USA.
authors: S
authors: S
authors: Dunston
authors: 2 Comparative Medicine Institute, NC State University, Raleigh, NC, USA.|4 Department of Population Health and Pathobiology, NC State University, College of Veterinary Medicine, Raleigh, NC, USA.
authors: K E
authors: KE
authors: Linder
authors: 5 Athens Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
authors: P
authors: P
authors: Rakich
authors: 2 Comparative Medicine Institute, NC State University, Raleigh, NC, USA.|3 Department of Clinical Sciences, NC State University, College of Veterinary Medicine, Raleigh, NC, USA.
authors: T
authors: T
authors: Olivry
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000502:physiology*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D003856:Deoxyuracil Nucleotides / Q000378:metabolism; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D015971:Gene Expression Regulation, Enzymologic; D020287:In Situ Nick-End Labeling; D015603:Keratinocytes / Q000473:pathology*; D013262:Stevens-Johnson Syndrome / Q000473:pathology / Q000662:veterinary*
chemical_list: D001059:Apoproteins; D003856:Deoxyuracil Nucleotides; C027078:deoxyuridine triphosphate; D004253:DNA Nucleotidylexotransferase; D053148:Caspase 3
keywords: apoptosis; erythema multiforme; skin; toxic epidermal necrolysis