title: Electropolymerized molecular imprinting on glassy carbon electrode for voltammetric detection of dopamine in biological samples.
abstract: A simple and reliable method for preparing a selective dopamine (DA) sensor based on a molecularly imprinted polymer of ethacridine was proposed. The molecularly imprinted polymer electrode was prepared through electrodepositing polyethacridine-dopamine film on the glassy carbon electrode and then removing DA from the film via chemical induced elution. The molecular imprinted sensor was tested by cyclic voltammetry as well as by differential pulse voltammetry (DPV) to verify the changes in oxidative currents of DA. In optimized DPV conditions the oxidation peak current was well-proportional to the concentration of DA in the range from 2.0×10(-8)M up to 1×10(-6)M. The limit of detection (3σ) of DA was found to be as low as 4.4nM, by the proposed sensor that could be considered a sensitive marker of DA depletion in Parkinson's disease. Good reproducibility with relative standard deviation of 1.4% and long term stability within two weeks were also observed. The modified sensor was validated for the analysis of DA in deproteinized human serum samples using differential pulse voltammetric technique.
authors: University of Pécs, Szentágothai János Research Center, Pécs, Ifjúság útja 20, 7624 Hungary.
authors: Laszlo
authors: L
authors: Kiss
authors: University of Bucharest, Faculty of Chemistry, Department of Analytical Chemistry, 90-92 Panduri Avenue, District 5, Bucharest, 050663 Romania.
authors: Vasile
authors: V
authors: David
authors: University of Bucharest, Faculty of Chemistry, Department of Analytical Chemistry, 90-92 Panduri Avenue, District 5, Bucharest, 050663 Romania.
authors: Iulia Gabriela
authors: IG
authors: David
authors: University of Bucharest, Faculty of Chemistry, Department of Analytical Chemistry, 90-92 Panduri Avenue, District 5, Bucharest, 050663 Romania.
authors: Paul
authors: P
authors: Lazăr
authors: University of Bucharest, Faculty of Chemistry, Department of Physical Chemistry, 4-12 Regina Elisabeta, District 3, Bucharest, 030018 Romania.
authors: Constantin
authors: C
authors: Mihailciuc
authors: University of Bucharest, Faculty of Physics, 3nano-SAE Research Centre, MG38-Magurele, Bucharest, Romania.
authors: Ioan
authors: I
authors: Stamatin
authors: University of Medicine and Pharmacy, Carol Davila, Department of Psychiatry, Bucharest, Romania.
authors: Adela
authors: A
authors: Ciobanu
authors: National Institute of Aerospace Medicine, 88 Mircea Vulcanescu Street, Bucharest, Romania.
authors: Cristian Dragoş
authors: CD
authors: Ştefănescu
authors: University of Pécs, Szentágothai János Research Center, Pécs, Ifjúság útja 20, 7624 Hungary.
authors: Livia
authors: L
authors: Nagy
authors: University of Pécs, Szentágothai János Research Center, Pécs, Ifjúság útja 20, 7624 Hungary.
authors: Géza
authors: G
authors: Nagy
authors: University of Bucharest, Faculty of Chemistry, Department of Analytical Chemistry, 90-92 Panduri Avenue, District 5, Bucharest, 050663 Romania. Electronic address: anton_ciucu@yahoo.com.
authors: Anton Alexandru
authors: AA
authors: Ciucu
mesh_terms: D002244:Carbon / Q000737:chemistry; D004298:Dopamine / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D004975:Ethacridine / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D054802:Molecular Imprinting; D058105:Polymerization; D011108:Polymers / Q000737:chemistry
chemical_list: D011108:Polymers; D002244:Carbon; D004298:Dopamine; D004975:Ethacridine
keywords: Biological samples; Dopamine; Glassy carbon; Molecularly imprinted polymer; Polyethacridine; Voltammetry