title: Simultaneous determination of three active components in rat plasma by UPLC-MS/MS: Application to pharmacokinetic study after oral administration of Herba Sarcandrae extract.
abstract: A rapid, specific and sensitive ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for determination of isofraxidin, rosmarinic acid and kaempferol-3-O-glucuronide in rat plasma using warfarin as an internal standard (IS). Separation was conducted on a Thermo Hypersil GOLD C18column with linear gradient elution using methanol and water. Mass spectrometric detection was conducted using selected reaction monitoring (SRM) via an electrospray ionization (ESI) source. All analytes exhibited good linearity within their concentration ranges (r > 0.9990). The lower limits of quantitations of isofraxidin, rosmarinic acid, and kaempferol-3-O-glucuronide were 1.31, 0.67 and 0.92 ng/mL, respectively. Intra- and inter-day precisions of these investigated components exhibited an RSD within 11.7%, and the accuracy ranged from -12.5 to 15.0% at all QC levels. The developed method was successfully applied to a pharmacokinetic study of isofraxidin, rosmarinic acid, and kaempferol-3-O-glucuronide in rats after oral administration of Herba Sarcandrae Extract.
authors: Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, China.
authors: Bin
authors: B
authors: Zhang
authors: Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, China.
authors: Jianguo
authors: J
authors: Chen
authors: Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, China.
authors: Qichuan
authors: Q
authors: Jiang
authors: Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, China.
authors: Xuefeng
authors: X
authors: Wang
authors: Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, China.
authors: Yan
authors: Y
authors: Lu
authors: Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, China.
authors: Liang
authors: L
authors: Gong
authors: Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, China.
authors: Dong
authors: D
authors: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D002934:Cinnamates / Q000097:blood; D003374:Coumarins / Q000097:blood; D053630:Depsides / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D020719:Glucuronides / Q000097:blood; D044949:Kaempferols / Q000097:blood; D057230:Limit of Detection; D019684:Magnoliopsida / Q000737:chemistry; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014859:Warfarin / Q000097:blood; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D003374:Coumarins; D053630:Depsides; D004365:Drugs, Chinese Herbal; D020719:Glucuronides; D044949:Kaempferols; D010936:Plant Extracts; D014859:Warfarin; D000097123:Rosmarinic Acid; C000615701:kaempferol-3-O-glucuronide; C008182:isofraxidin
keywords: Herba Sarcandrae; UPLC-MS/MS; pharmacokinetic study; rat plasma