title: Perfluorooctanoic acid induces human Ishikawa endometrial cancer cell migration and invasion through activation of ERK/mTOR signaling.
abstract: Perfluorooctanoic acid (PFOA) is a common environmental pollutant that has been associated with various diseases, including cancer. We explored the molecular mechanisms underlying PFOA-induced endometrial cancer cell invasion and migration. PFOA treatment enhanced migration and invasion by human Ishikawa endometrial cancer cells, which correlated with decreased E-cadherin expression, a marker of epithelial-mesenchymal transition. PFOA also induced activation of ERK1/2/mTOR signaling. Treatment with rapamycin, an mTOR inhibitor, antagonized the effects of PFOA and reversed the effects of PFOA activation in a xenograft mouse model of endometrial cancer. Consistent with these results, pre-treatment with rapamycin abolished PFOA-induced down-regulation of E-cadherin expression. These results indicate that PFOA is a carcinogen that promotes endometrial cancer cell migration and invasion through activation of ERK/mTOR signaling.
authors: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.|Department of Obstetrics and Gynecology, Yangzhou Maternal and Child Health Hospital, Yangzhou University, Yangzhou, China.|State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
authors: Zhinan
authors: Z
authors: Ma
authors: Key Laboratory of Pathogen Biology of Jiangsu Province, Nanjing Medical University, Nanjing, China.|Department of Microbiology, Nanjing Medical University, Nanjing, China.
authors: Xiaoqiu
authors: X
authors: Liu
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
authors: Fujun
authors: F
authors: Li
authors: Department of Obstetrics and Gynecology, Yangzhou Maternal and Child Health Hospital, Yangzhou University, Yangzhou, China.
authors: Yixong
authors: Y
authors: Wang
authors: Department of Obstetrics and Gynecology, Yangzhou Maternal and Child Health Hospital, Yangzhou University, Yangzhou, China.
authors: Yang
authors: Y
authors: Xu
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
authors: Mei
authors: M
authors: Zhang
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
authors: Xiaoqian
authors: X
authors: Zhang
authors: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Xiaoyan
authors: X
authors: Ying
authors: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
authors: Xuesen
authors: X
authors: Zhang
mesh_terms: D000818:Animals; D002210:Caprylates / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000473:pathology*; D004789:Enzyme Activation / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D005260:Female; D005466:Fluorocarbons / Q000633:toxicity*; D064593:Heterografts; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000473:pathology; D015398:Signal Transduction / Q000187:drug effects; D058570:TOR Serine-Threonine Kinases / Q000187:drug effects* / Q000378:metabolism
chemical_list: D002210:Caprylates; D002273:Carcinogens; D005466:Fluorocarbons; C023036:perfluorooctanoic acid; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases
keywords: ERK/mTOR; PFOA; endometrial carcinoma; migration and invasion; tumorigenesis