title: Development of a UPLC-MS/MS method for the determination of lomefloxacin in rabbit aqueous humor and its application to a pharmacokinetic study.
abstract: Lomefloxacin is a kind of synthetic fluoroquinolone antibiotic, which is used for the treatment of infectious diseases. In this study, a rapid and efficient liquid chromatography-tandem mass spectrometric assay was developed to determine the concentration of lomefloxacin in rabbit aqueous humor quantitatively. Aqueous humor samples were extracted by protein precipitation. Ofloxacin was chosen as internal standard. The chromatographic separation was achieved on a Kinetex C18 (50mm×2.10mm, 2.6μm, Phenomenex Corp, USA) column, with a gradient of methanol (0.1% formic acid) and water (0.1% formic acid). Multiple reaction monitoring (MRM) with positive ionization mode was used for the mass analysis. The validation of this method was based on the European Medicines Agency (2011) [1] and US FDA Guidelines (2001) [2]. The calibration range of aqueous humor samples was 5-1200ng/mL with r=0.9990 (n=6). For all QC samples, Inter-and intra-run precisions were less than 15% and accuracies were between 80%-120%. In conclusion, the assay was rapid, sensitive and able to determinate the lomefloxacin in rabbit aqueous humor accurately. At the same time, this method was successfully applied to study the pharmacokinetics of lomefloxacin hydrochloride eye drops and lomefloxacin hydrochloride ophthalmic gel in rabbit aqueous humor.
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors: Shiwen
authors: S
authors: Song
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors: Dongyang
authors: D
authors: Zhao
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors: Jing
authors: J
authors: Sun
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors: Qiuyan
authors: Q
authors: Miao
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors: Xiaojie
authors: X
authors: Liu
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors: Yanling
authors: Y
authors: Wang
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors: Lu
authors: L
authors: Zhong
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors: Meng
authors: M
authors: Xu
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: zhangpengspu@163.com.
authors: Peng
authors: P
authors: Zhang
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D024841:Fluoroquinolones / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D015242:Ofloxacin / Q000032:analysis; D009883:Ophthalmic Solutions / Q000493:pharmacokinetics; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D024841:Fluoroquinolones; D009883:Ophthalmic Solutions; D015242:Ofloxacin; C053091:lomefloxacin
keywords: Eye drops; Lomefloxacin; Pharmacokinetics; Rabbit aqueous humor; UPLC–MS/MS