title: Various calibration procedures result in optimal standardization of routinely used 25(OH)D ID-LC-MS/MS methods.
abstract: BACKGROUND
  The variety of LC-MS/MS methods measuring total 25(OH)D used today is vast and the comparability among these methods is still not well assessed.
  
  
  METHODS
  Here, we performed a comparison in samples of healthy donors between the currently routinely used 25(OH)D LC-MS/MS methods in the Netherlands and the Ghent University reference measurement procedure to address this issue (n=40). Additionally, an interlaboratory comparison in patient serum samples assessed agreement between the Dutch diagnostic methods (n=37).
  
  
  RESULTS
  The overall correlation of the routine methods for 25(OH)D3with the reference measurement procedures and with the mean of all diagnostic methods was excellent (r>0.993 and r>0.989, respectively). Three out of five methods aligned perfectly with both the reference measurement procedure and the median of all methods. One of the routine methods showed a small positive bias, while another showed a small negative bias consistently in both comparisons.
  
  
  CONCLUSIONS
  The biases most probably originated from differences in calibration procedure and may be obviated by reassessing calibration of stock standards and/or calibrator matrices. In conclusion, five diagnostic centers have performed a comparison with the 25(OH)D Ghent University reference measurement procedure in healthy donor serum samples and a comparison among themselves in patient serum samples. Both analyses showed a high correlation and specificity of the routine LC-MS/MS methods, yet did reveal some small standardization issues that could not be traced back to the technical details of the different methods. Hence, this study indicates various calibration procedures can result in perfect alignment.
authors: Department of Clinical Chemistry, Endocrine Laboratory, VU University Medical Center, Amsterdam, The Netherlands.
authors: Niek F
authors: NF
authors: Dirks
authors: Centers for Disease Control and Prevention (CDC), Division of Laboratory Sciences, Atlanta, GA, United States.
authors: Hubert W
authors: HW
authors: Vesper
authors: Department of Laboratory Medicine, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors: Antonius E
authors: AE
authors: van Herwaarden
authors: Department of Clinical Chemistry, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands.
authors: Jody M W
authors: JM
authors: van den Ouweland
authors: Department of Laboratory Medicine, University of Groningen, University Medical Centre Groningen, Groningen, The Netherlands.
authors: Ido P
authors: IP
authors: Kema
authors: Department of Clinical Chemistry and Laboratory Medicine, Medical Spectrum Twente, Medlon BV, The Netherlands.
authors: Johannes G
authors: JG
authors: Krabbe
authors: Department of Clinical Chemistry, Endocrine Laboratory, VU University Medical Center, Amsterdam, The Netherlands. Electronic address: a.heijboer@vumc.nl.
authors: Annemieke C
authors: AC
authors: Heijboer
mesh_terms: D015652:25-Hydroxyvitamin D 2 / Q000097:blood*; D002112:Calcifediol / Q000097:blood*; D002138:Calibration; D002853:Chromatography, Liquid; D064368:Healthy Volunteers; D006801:Humans; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards*
chemical_list: D015652:25-Hydroxyvitamin D 2; D002112:Calcifediol
keywords: Mass spectrometry; Method comparison; Standardization; Vitamin D