title: Molecular signatures associated with ZIKV exposure in human cortical neural progenitors.
abstract: Zika virus (ZIKV) infection causes microcephaly and has been linked to other brain abnormalities. How ZIKV impairs brain development and function is unclear. Here we systematically profiled transcriptomes of human neural progenitor cells exposed to Asian ZIKVC, African ZIKVM, and dengue virus (DENV). In contrast to the robust global transcriptome changes induced by DENV, ZIKV has a more selective and larger impact on expression of genes involved in DNA replication and repair. While overall expression profiles are similar, ZIKVC, but not ZIKVM, induces upregulation of viral response genes and TP53. P53 inhibitors can block the apoptosis induced by both ZIKVCand ZIKVMin hNPCs, with higher potency against ZIKVC-induced apoptosis. Our analyses reveal virus- and strain-specific molecular signatures associated with ZIKV infection. These datasets will help to investigate ZIKV-host interactions and identify neurovirulence determinants of ZIKV.
authors: Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors: Feiran
authors: F
authors: Zhang
authors: Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
authors: Christy
authors: C
authors: Hammack
authors: Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
authors: Sarah C
authors: SC
authors: Ogden
authors: Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
authors: Yichen
authors: Y
authors: Cheng
authors: Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
authors: Emily M
authors: EM
authors: Lee
authors: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA Departments of Psychiatry and Behavioral Science, Cell Biology, and Neurology, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors: Zhexing
authors: Z
authors: Wen
authors: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA Biomedical Engineering Graduate Program, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors: Xuyu
authors: X
authors: Qian
authors: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors: Ha Nam
authors: HN
authors: Nguyen
authors: Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors: Yujing
authors: Y
authors: Li
authors: Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors: Bing
authors: B
authors: Yao
authors: National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892, USA.
authors: Miao
authors: M
authors: Xu
authors: Department of Biostatistics and Bioinformatics, Emory University Rollins School of Public Health, Atlanta, GA 30322, USA.
authors: Tianlei
authors: T
authors: Xu
authors: Department of Biostatistics and Bioinformatics, Emory University Rollins School of Public Health, Atlanta, GA 30322, USA.
authors: Li
authors: L
authors: Chen
authors: Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors: Zhiqin
authors: Z
authors: Wang
authors: Department of Biostatistics and Bioinformatics, Emory University Rollins School of Public Health, Atlanta, GA 30322, USA.
authors: Hao
authors: H
authors: Feng
authors: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors: Wei-Kai
authors: WK
authors: Huang
authors: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors: Ki-Jun
authors: KJ
authors: Yoon
authors: Department of Biochemistry & Molecular Biology, Department of Pharmacology & Toxicology, Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Chao
authors: C
authors: Shan
authors: Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors: Luoxiu
authors: L
authors: Huang
authors: Department of Biostatistics and Bioinformatics, Emory University Rollins School of Public Health, Atlanta, GA 30322, USA.
authors: Zhaohui
authors: Z
authors: Qin
authors: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors: Kimberly M
authors: KM
authors: Christian
authors: Department of Biochemistry & Molecular Biology, Department of Pharmacology & Toxicology, Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX 77555, USA.
authors: Pei-Yong
authors: PY
authors: Shi
authors: Sylvester Comprehensive Cancer Center, Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
authors: Mingjiang
authors: M
authors: Xu
authors: National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892, USA.
authors: Menghang
authors: M
authors: Xia
authors: National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892, USA.
authors: Wei
authors: W
authors: Zheng
authors: Department of Biostatistics and Bioinformatics, Emory University Rollins School of Public Health, Atlanta, GA 30322, USA.
authors: Hao
authors: H
authors: Wu
authors: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA The Solomon Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA shongju1@jhmi.edu.
authors: Hongjun
authors: H
authors: Song
authors: Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA tang@bio.fsu.edu.
authors: Hengli
authors: H
authors: Tang
authors: Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA The Solomon Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA gming1@jhmi.edu.
authors: Guo-Li
authors: GL
authors: Ming
authors: Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA peng.jin@emory.edu.
authors: Peng
authors: P
authors: Jin
mesh_terms: D016923:Cell Death / Q000235:genetics; D002460:Cell Line; D002540:Cerebral Cortex / Q000166:cytology*; D004260:DNA Repair / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D003716:Dengue Virus / Q000502:physiology; D020869:Gene Expression Profiling; D006801:Humans; D058953:Neural Stem Cells / Q000378:metabolism* / Q000821:virology*; D015398:Signal Transduction / Q000235:genetics; D013045:Species Specificity; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics; D000071244:Zika Virus / Q000502:physiology*; D000071243:Zika Virus Infection / Q000235:genetics* / Q000821:virology
chemical_list: D016159:Tumor Suppressor Protein p53