title: Diagnostic value of immunohistochemistry for the detection of the BRAF V600E mutation in colorectal carcinoma.
abstract: OBJECTIVE
  V600E is the most common activating BRAF mutation in colorectal carcinomas (CRCs). It is a crucial biomarker for patient selection and response to targeted therapy with BRAF V600E inhibitors. Previous studies using immunohistochemistry (IHC) have shown different results. In this study, we evaluated the IHC expression of the mutated BRAF protein in archival material from CRC specimens and correlated it with DNA sequence analysis.
  
  
  METHODS
  51 cases of primary colon adenocarcinoma were stained with BRAF V600E-specific clone VE1 antibody against mutated BRAF protein. DNA sequence analysis was performed and the results were compared.
  
  
  RESULTS
  BRAF V600E protein was detected in the cytoplasm of neoplastic cells in 15 of the 51 examined cases (29.4%). The correlation between IHC staining and DNA sequence analysis showed 93.75% sensitivity and 100% specificity.
  
  
  CONCLUSIONS
  Our data show that IHC could be used in routine clinical practice as a screening method for BRAF V600E mutant protein detection in CRC patients.
authors: Department of Pathology, University of Thessaly, Faculty of Medicine, Biopolis 41110, Larissa, Greece.
authors: Maria
authors: M
authors: Ioannou
authors: Roidoula
authors: R
authors: Papamichali
authors: Maria
authors: M
authors: Samara
authors: Efrosini
authors: E
authors: Paraskeva
authors: Constantina
authors: C
authors: Papacharalambous
authors: Korina
authors: K
authors: Baxevanidou
authors: George
authors: G
authors: Koukoulis
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009154:Mutation; D048493:Proto-Oncogene Proteins B-raf / Q000032:analysis / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics; D012189:Retrospective Studies; D017422:Sequence Analysis, DNA
chemical_list: C117307:KRAS protein, human; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf; D016283:Proto-Oncogene Proteins p21(ras)