title: Gene Expression Profile Regulated by CREB in K562 Cell Line.
abstract: BACKGROUND
  Cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) is a member of the CREB/activation transcription factor 1 family that binds to an octanucleotide cAMP response element consensus sequence in promoters of target genes.
  
  
  METHODS
  CREB has been shown to regulate a variety of cellular functions, including cell proliferation, survival, apoptosis, differentiation, metabolism, hematopoiesis, immune response, and neuronal activity. CREB was also identified as a proto-oncogene involving in transformation by promoting abnormal proliferation and survival of myeloid cells. To understand the mechanism of CREB functions in leukemogenesis, the transcriptional profiles from a K562 cell line in which CREB was knocked down were analyzed with the use of bioinformatics methods.
  
  
  RESULTS
  DAVID Bioinformatics Resources and Gene Set Enrichment Analysis (GSEA) were performed to identify the targets that are regulated by CREB. A total of 692 genes were up-regulated and 364 genes down-regulated. The up-regulated genes were significantly enriched in pathways of cancer and chronic myeloid leukemia. GSEA analysis showed expression of Notch1 pathway to be decreased and hypoxia-inducible factor (HIF) pathway to be activated.
  
  
  CONCLUSIONS
  Our results identified candidate gene sets that could be used to guide research on discovering the mechanism of CREB during leukemogenesis.
authors: Department of Blood Transfusion, Dongtai People's Hospital of Jiangsu Province, Dongtai, Jiangsu, People's Republic of China. Electronic address: xiahefeng_js@163.com.
authors: H
authors: H
authors: Xia
authors: Department of Hematology, Dongtai People's Hospital of Jiangsu Province, Dongtai, Jiangsu, People's Republic of China.
authors: Z
authors: Z
authors: Gong
authors: Department of Central Laboratory, Dongtai People's Hospital of Jiangsu Province, Dongtai, Jiangsu, People's Republic of China.
authors: Y
authors: Y
authors: Lian
authors: Department of Cardiology, Dongtai People's Hospital of Jiangsu Province, Dongtai, Jiangsu, People's Republic of China.
authors: J
authors: J
authors: Zhou
authors: Department of Hematology, Dongtai People's Hospital of Jiangsu Province, Dongtai, Jiangsu, People's Republic of China.
authors: X
authors: X
authors: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000235:genetics; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics*; D015536:Down-Regulation; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D006410:Hematopoiesis / Q000235:genetics; D006801:Humans; D020014:K562 Cells; D011401:Promoter Regions, Genetic; D000090063:Proto-Oncogene Mas; D014157:Transcription Factors / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics; D059467:Transcriptome / Q000235:genetics*; D015854:Up-Regulation
chemical_list: C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D014157:Transcription Factors