title: Long non-coding RNA UCA1 promotes the tumorigenesis in pancreatic cancer.
abstract: The contribution of long non-coding RNAs (lncRNAs) to tumorigenesis and metastasis of pancreatic cancer (PC) remains largely unknown. Urothelial cancer-associated 1 (UCA1), which is an originally identified lncRNA in bladder cancer, has be proved to play a pivotal role in bladder cancer progression and embryonic development. In this study, we detected the mRNA expression of UCA1 in 128 PC patients by qRT-PCR, and found that UCA1 expression was significantly, up-regulated in tumor tissues than that in matched adjacent non-tumor tissues (p<0.05). Clinicopathological analysis demonstrated that UCA1 expression in PC significantly correlated with malignant potential factors such as tumor size (p=0.021), depth of invasion (p=0.033), CA19-9 level (p=0.034) and tumor stage (p=0.013). Cox proportional hazards regression analysis also confirmed that high UCA1 expression was an independent prognostic biomarker of PC (p=0.046), which led to an obviously shorter 5-year overall survival (OS) compared to those patients with low UCA1 expressions (p=0.018). Furthermore, we effectively down-regulated UCA1 mRNA expression by transfecting RNA interfere fragments into SW-1990 cells, and our results in vitro indicated that down-regulation of UCA1 could effectively inhibit the cell proliferative activities, induce apoptotic rate and cause cell cycle arrest in PC cells (p<0.05). Meanwhile, UCA1 expression negative-correlated with p27 in PC tissues (r2=0.46, p<0.01), and knockdown of p27 partly abrogated the cell proliferative activities caused by UCA1 (p<0.05). Our results raised the possibility of using UCA1 as a potential prognostic biomarker and therapy target of PC, and down-regulation of UCA1 might be considered to be a novel molecular treatment strategy for patients with PC.
authors: Department of General Surgery, Ningbo No. 2 Hospital, Ningbo 315000, China.
authors: Ping
authors: P
authors: Chen
authors: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.
authors: Daiwei
authors: D
authors: Wan
authors: Department of General Surgery, Ningbo No. 2 Hospital, Ningbo 315000, China.
authors: Dingcheng
authors: D
authors: Zheng
authors: Department of General Surgery, Ningbo No. 2 Hospital, Ningbo 315000, China.
authors: Qi
authors: Q
authors: Zheng
authors: Department of General Surgery, Ningbo No. 2 Hospital, Ningbo 315000, China. Electronic address: gastroclinical@sina.com.
authors: Feng
authors: F
authors: Wu
authors: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China. Electronic address: strexboy@163.com.
authors: Qiaoming
authors: Q
authors: Zhi
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology*; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; C515159:UCA1 RNA, human
keywords: Long non-coding RNA; Pancreatic cancer; Prognosis; UCA1; p27