title: DNA Damage Detection by 53BP1: Relationship to Species Longevity.
abstract: In order to examine potential differences in genomic stability, we have challenged fibroblasts derived from five different mammalian species of variable longevity with the genotoxic agents, etoposide and neocarzinostatin. We report that cells from longer-lived species exhibit more tumor protein p53 binding protein 1 (53BP1) foci for a given degree of DNA damage relative to shorter-lived species. The presence of a greater number of 53BP1 foci was associated with decreased DNA fragmentation and a lower percentage of cells exhibiting micronuclei. These data suggest that cells from longer-lived species have an enhanced DNA damage response. We propose that the number of 53BP1 foci that form in response to damage reflects the intrinsic capacity of cells to detect and respond to DNA harms.
authors: Department for Life Quality Studies and.
authors: Eleonora
authors: E
authors: Croco
authors: Department of Biomedical and Neuromotor Sciences, University of Bologna, Italy.
authors: Silvia
authors: S
authors: Marchionni
authors: Department of Biomedical and Neuromotor Sciences, University of Bologna, Italy.
authors: Martine
authors: M
authors: Bocchini
authors: Department for Life Quality Studies and.
authors: Cristina
authors: C
authors: Angeloni
authors: The Lankenau Institute for Medical Research, Wynnewood, Pennsylvania.
authors: Thomas
authors: T
authors: Stamato
authors: Department for Life Quality Studies and.
authors: Claudio
authors: C
authors: Stefanelli
authors: Department for Life Quality Studies and.
authors: Silvana
authors: S
authors: Hrelia
authors: Department of Pathology, Drexel University College of Medicine, Philadelphia, Pennsylvania.
authors: Christian
authors: C
authors: Sell
authors: Department of Biomedical and Neuromotor Sciences, University of Bologna, Italy.
authors: Antonello
authors: A
authors: Lorenzini
mesh_terms: D000818:Animals; D002417:Cattle; D059447:Cell Cycle Checkpoints; D002460:Cell Line; D002685:Chiroptera; D019925:Cyclin A / Q000378:metabolism; D003603:Cytotoxins / Q000633:toxicity; D004249:DNA Damage; D053938:DNA Fragmentation; D004285:Dogs; D005047:Etoposide / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D042822:Genomic Instability; D006657:Histones / Q000378:metabolism; D006801:Humans; D008017:Life Expectancy; D008136:Longevity; D051379:Mice; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D000072379:NIMA-Related Kinases / Q000378:metabolism; D059005:Topoisomerase II Inhibitors / Q000633:toxicity; D000071857:Tumor Suppressor p53-Binding Protein 1 / Q000378:metabolism*; D009353:Zinostatin / Q000633:toxicity
chemical_list: D019925:Cyclin A; D003603:Cytotoxins; D005047:Etoposide; D006657:Histones; D000072379:NIMA-Related Kinases; D059005:Topoisomerase II Inhibitors; D000071857:Tumor Suppressor p53-Binding Protein 1; D009353:Zinostatin; C468783:H2AX protein, human; C547589:NEK4 protein, human
keywords: 53BP1; DNA damage foci; Genomic stability; Longevity; Micronuclei