title: Rapid preparation of functional polysaccharides from Pyropia yezoensis by microwave-assistant rapid enzyme digest system.
abstract: This study describes a simple preparation of functional polysaccharides from Pyropia yezoensis using a microwave-assistant rapid enzyme digest system (MAREDS) with various carbohydrases, and evaluates their antioxidative effects. Polysaccharide hydrolysates were prepared using MAREDS under different hydrolytic conditions of the carbohydrases and microwave powers. Polysaccharides less than 10kDa (Low molecular weight polysaccharides, LMWP, ≤10kDa) were efficiently obtained using an ultrafiltration (molecular weight cut-off of 10kDa). MAREDS increases AMG activation via an increased degree of hydrolysis; the best AMG hydrolysate was prepared using a 10:1 ratio of substrate to enzyme for 2h in MAREDS with 400W. LMWP consisted of galactose (27.3%), glucose (64.5%), and mannose (8.3%) from the AMG hydrolysate had stronger antioxidant effects than the high molecular weight polysaccharides (>10kDa). We rapidly prepared functional LMWPs by using MAREDS with carbohydrases, and suggest that LMWP might be potentially a valuable algal polysaccharide antioxidant.
authors: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Ji-Hyeok
authors: JH
authors: Lee
authors: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Hyung-Ho
authors: HH
authors: Kim
authors: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Ju-Young
authors: JY
authors: Ko
authors: Division of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, University of Pittsburgh and VA Pittsburgh Healthcare System, Pittsburgh, Pennsylvania, USA.
authors: Jun-Ho
authors: JH
authors: Jang
authors: Department of Biology, College of Natural Sciences, Kongju National University, Kongju 314-701, Republic of Korea.
authors: Gwang-Hoon
authors: GH
authors: Kim
authors: Industry-Academic Cooperation Foundation, Jeju National University, Jeju 690-756, Republic of Korea.
authors: Jung-Suck
authors: JS
authors: Lee
authors: Department of High Polymer Engineering, Sunchon National University, Jungang-ro, Suncheon, Jeollanam-do, Republic of Korea. Electronic address: jwnah@sunchon.ac.kr.
authors: Jae-Woon
authors: JW
authors: Nah
authors: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: youjin2014@gmail.com.
authors: You-Jin
authors: YJ
authors: Jeon
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005591:Chemical Fractionation; D008872:Microwaves; D009005:Monosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000461:Rhodophyta / Q000737:chemistry*; D014462:Ultrafiltration
chemical_list: D000975:Antioxidants; D009005:Monosaccharides; D011134:Polysaccharides
keywords: Antioxidative effect; Low molecular weight polysaccharides; Microwave-assisted rapid enzyme digest system; Pyropia yezoensis