title: Protective effect of infrared-A radiation against damage induced by UVB radiation in the melan-a cell line.
abstract: The present work evaluated the infrared-A (IR-A) protective effect using a light-emitting diode (LED) lamp against the cytotoxic effects of ultraviolet B radiation (UVB). Effects on cell viability (Trypan blue assay), DNA damage (comet assay), lipid peroxidation (FOX method), reactive oxygen species production and antioxidant capacity were analyzed in melan-a, a non-tumoral murine melanocytic cell line. To define the doses used in the interaction experiments between IR-A+UVB, dose/response curves were made after exposure to IR-A or UVB. The IR-A dose chosen was 0.8J/cm(2) because this dose caused no significant inhibition of proliferation effects and viability decreased. For UVB exposure, a dose of 0.015J/cm(2), which showed a decrease in viable cell number by approximately 50% in relation to control until 72h, was selected. For IR-A+UVB, cell proliferation recovery was showed, decreasing DNA damage and lipid peroxide content when compared to UVB alone. Besides, the results obtained for ROS and antioxidant capacity showed that the protection observed was probably not related to decreased oxidative stress. In conclusion, non-thermal IR-A was capable of protecting the melan-a cells from UVB induced damage.
authors: Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil. Electronic address: line.89@hotmail.com.
authors: Aline
authors: A
authors: Portantiolo Lettnin
authors: Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil. Electronic address: marianasantossalgado@yahoo.com.br.
authors: Mariana
authors: M
authors: Teixeira Santos Figueiredo Salgado
authors: Escola de Química e Alimentos, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil. Electronic address: camilacruz@furg.br.
authors: Camila
authors: C
authors: Gonsalez Cruz
authors: Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil. Electronic address: f.m.r.silvajunior@gmail.com.
authors: Flávio
authors: F
authors: Manoel Rodrigues da Silva-Júnior
authors: Instituto Multidisciplinar em Saúde - Campus Anísio Teixeira, Universidade Federal da Bahia, Vitória da Conquista, BA Brazil. Electronic address: binn05@gmail.com.
authors: Vinícius
authors: V
authors: Cunha Gonzalez
authors: Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil. Electronic address: anavotto@yahoo.com.br.
authors: Ana Paula
authors: AP
authors: de Souza Votto
authors: Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil. Electronic address: gilma.trindade@gmail.com.
authors: Gilma
authors: G
authors: Santos Trindade
authors: Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil. Electronic address: dazafil@gmail.com.
authors: Daza
authors: D
authors: de Moraes Vaz Batista Filgueira
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000528:radiation effects; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival; D004249:DNA Damage; D007259:Infrared Rays; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000528:radiation effects; D051379:Mice; D018384:Oxidative Stress / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species
keywords: Cell viability; DNA damage; Lipid peroxidation; Photoprotection; Radiation interaction; Skin photooxidative stress