title: Effects of sphingosylphosphorylcholine on cryopreserved fat tissue graft survival.
abstract: Autogenous microfat grafting is widely used to augment depressed deformities or for other cosmetic purposes. Since the microfat survival rate is unpredictable due to absorption and calcification, previously cryopreserved fat is widely used for secondary procedures. Sphingosylphosphorylcholine (SPC) is a lysophospholipid, which has a role in several cellular responses, and is known to stimulate DNA synthesis and proliferation. Since endothelial progenitor cells (EPCs) are known to enhance the survival rate of transplanted fat tissue, the present study assessed the effects of SPC on EPCs, in order to verify its positive effects on proliferation. Cryopreserved human fat tissues mixed with various concentrations of SPC were grafted into the nude mouse model. After grafting, the viability of each SPC mixed group was determined and compared with that of the non‑SPC group. SPC exhibited a positive effect on EPC proliferation and angiogenic potential over 3 days when used at specific concentrations. The fat grafts of the 3 µM SPC‑treated group weighed significantly more and the volume was markedly increased, as compared with the control group. A reverse transcription‑quantitative polymerase chain reaction was conducted on the total RNA extracted from SPC‑treated fat tissues, which detected increased mRNA expression levels of matrix metallopeptidase‑9 and tumor necrosis factor‑α compared with in the control group. These results indicate that specific concentrations of SPC may exert favorable effects on grafted cryopreserved human fat tissue, which may be due to the increased mRNA expression levels of genes associated with angiogenesis.
authors: Department of Plastic and Reconstructive Surgery, School of Medicine, Pusan National University, Busan 602‑739, Republic of Korea.
authors: Yong Chan
authors: YC
authors: Bae
authors: Department of Plastic and Reconstructive Surgery, School of Medicine, Pusan National University, Busan 602‑739, Republic of Korea.
authors: Chi Won
authors: CW
authors: Choi
authors: Department of Plastic and Reconstructive Surgery, School of Medicine, Pusan National University, Busan 602‑739, Republic of Korea.
authors: Kyeong Wook
authors: KW
authors: Nam
authors: Biomedical Research Institute, Pusan National University Hospital, Busan 602‑739, Republic of Korea.
authors: Ji Sun
authors: JS
authors: Song
authors: Department of Plastic and Reconstructive Surgery, School of Medicine, Pusan National University, Busan 602‑739, Republic of Korea.
authors: Jae Woo
authors: JW
authors: Lee
mesh_terms: D000273:Adipose Tissue / Q000098:blood supply / Q000187:drug effects / Q000637:transplantation*; D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation; D015925:Cryopreservation; D066026:Endothelial Progenitor Cells / Q000166:cytology / Q000187:drug effects; D006085:Graft Survival / Q000187:drug effects*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D043925:Angiogenesis Inducing Agents; C005356:sphingosine phosphorylcholine; D010767:Phosphorylcholine; D013110:Sphingosine