title: Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel metal resistant bacterium Cupriavidus sp. S1.
abstract: A novel heterotrophic nitrifying and metal resistant bacterium was isolated and identified as Cupriavidus sp. S1. The utilization of ammonium, nitrate and nitrite as well as the production of N2 proved the heterotrophic nitrification and aerobic denitrification ability of S1. The ammonium, nitrate and nitrite removal efficiencies were 99.68%, 98.03% and 99.81%, with removal rates of 10.43, 8.64 and 8.36mg/L/h, respectively. A multiple regression equation well described the relationship between carbon source utilization, cell growth and nitrification. Keeping the shaking speed at 120rpm was beneficial for denitrification. Moreover, different forms of nitrogen source could be utilize in simultaneous nitrification and denitrification. Additionally, the efficient removal of ammonium occurred at 20.0mg/LZn(2+), or 10.0mg/LNi(2+) or 8.0mg/LCu(2+) or 5.0mg/LCr(6+), 33.35mmol/L sodium pyruvate, C/N 12-28. These findings demonstrate that S1 was effective for nitrogen removal in industrial wastewater containing heavy metal.
authors: Key Laboratory of Coal Science and Technology, Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China.
authors: Zhiyi
authors: Z
authors: Sun
authors: Key Laboratory of Coal Science and Technology, Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China. Electronic address: lykang@tyut.edu.cn.
authors: Yongkang
authors: Y
authors: Lv
authors: College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China.
authors: Yuxiang
authors: Y
authors: Liu
authors: Key Laboratory of Coal Science and Technology, Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan 030024, Shanxi, China.
authors: Ruipeng
authors: R
authors: Ren
mesh_terms: D000332:Aerobiosis; D064751:Ammonium Compounds / Q000302:isolation & purification; D049921:Cupriavidus / Q000378:metabolism*; D058440:Denitrification; D052836:Heterotrophic Processes; D008670:Metals; D009566:Nitrates / Q000302:isolation & purification; D058465:Nitrification; D009573:Nitrites / Q000302:isolation & purification; D009584:Nitrogen / Q000302:isolation & purification / Q000378:metabolism*; D062065:Wastewater
chemical_list: D064751:Ammonium Compounds; D008670:Metals; D009566:Nitrates; D009573:Nitrites; D009584:Nitrogen; D062065:Wastewater
keywords: Aerobic denitrification; Cupriavidus sp. S1; Heavy metal; Heterotrophic nitrification; Nitrogen removal