title: Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm.
abstract: This study was conducted to evaluate the effects of interacting seminal plasma proteins (iSPP) obtained by AV or EE on frozen-thawed ram sperm in order to test the hypothesis whether this fraction could be sufficient to emulate the effect of complete seminal plasma (SP). Additionally, we evaluated whether these proteins have a differential effect between spermatozoa from high and low fertility rams and between breeding and non-breeding seasons. We assessed sperm motility, quality parameters (intracellular reactive oxygen species, membrane fluidity, plasma membrane permeability and mitochondrial activity) and capacitation status. The main findings from this work were: i) iSPP had no effect on sperm motility, whereas SP (AV or EE) addition produced the highest values of total motility (74.13±2.99 and 72.27±2.99 for AV and EE, respectively) and progressive motility (64.97±2.64 and 63.73±2.64 for AV and EE, respectively); ii) iSPP had no effect on sperm quality parameters (p>0.05), but whole SP improved all parameters evaluated. Moreover, SP collected by AV yielded significantly higher viability (44.60±2.87) and sperm with stable plasma membrane (44.56±2.49) comparing with the addition of SP collected by EE (35.80±2.47 and 36.67±1.71, respectively); iii) iSPP and SP collected by EE, but not by AV, reverted molecular signals of capacitation as protein tyrosine phosphorylation caused by freezing temperatures; iv) there were no effects of fertility or season in sperm quality parameters evaluated. This study demonstrated that, although the iSPP have a clear decapacitating effect, including the ability to revert cryo-capacitation indicators, they are not sufficient to emulate the effects of complete SP regarding sperm functional parameters.
authors: Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Ruta 226km 73.5, Balcarce, 7620, Argentina.
authors: Alba
authors: A
authors: Ledesma
authors: Instituto de Desarrollo Ganadero y Sanidad Animal (INDEGSAL) y Departamento de Biología Molecular (Biología Celular), Universidad de León, Campus de Vegazana 24071, León, Spain.
authors: Estela
authors: E
authors: Fernández-Alegre
authors: Biotecnología de la Reproducción, Departamento de Producción Animal, Instituto Nacional de Tecnología Agropecuaria (INTA), Balcarce, Argentina.
authors: Adriana
authors: A
authors: Cano
authors: Biotecnología de la Reproducción, Departamento de Producción Animal, Instituto Nacional de Tecnología Agropecuaria (INTA), Balcarce, Argentina. Electronic address: fhozbor@hotmail.com.
authors: Federico
authors: F
authors: Hozbor
authors: Instituto de Desarrollo Ganadero y Sanidad Animal (INDEGSAL) y Departamento de Biología Molecular (Biología Celular), Universidad de León, Campus de Vegazana 24071, León, Spain.
authors: Felipe
authors: F
authors: Martínez-Pastor
authors: Biología de Microorganismos y Gametas, Instituto de Investigaciones Biológicas-CONICET, Universidad Nacional de Mar del Plata, Funes 3250, Mar del Plata, 7600, Argentina.
authors: Andreína
authors: A
authors: Cesari
mesh_terms: D000818:Animals; D015415:Biomarkers; D015925:Cryopreservation / Q000662:veterinary*; D005615:Freezing; D008297:Male; D012661:Semen / Q000502:physiology*; D055101:Semen Analysis / Q000662:veterinary; D029589:Seminal Plasma Proteins / Q000494:pharmacology*; D012756:Sheep / Q000502:physiology*; D013075:Sperm Capacitation / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D015415:Biomarkers; D029589:Seminal Plasma Proteins
keywords: Capacitation; Post-thaw sperm quality; Seminal plasma