title: Formulation and physiochemical study of α-tocopherol based oil in water nanoemulsion stabilized with non toxic, biodegradable surfactant: Sodium stearoyl lactate.
abstract: The unique properties such as high optical clarity, stability and enhanced bioavailability of nanoemulsion make them useful for food, cosmetic and pharmaceutical industries. In this work, sodium stearoyl lactate and Tween 80 surfactants were collectively used to fabricate alpha tocopherol based oil in water nanoemulsion using high energy ultrasonication method. The spherical nature of pure and drug loaded nanoemulsion has been confirmed with transmission electron microscopy (TEM). The influence of pH, dilution, surfactant concentration and ionic strength on average particle size of pure and nutraceutical (benzylisothiocyanate and curcumin) encapsulated emulsion was examined. The prepared emulsion exhibited good stability up to 90days in salt solution (50-200mM) and different pH conditions. The cumulative release % of benzylisothiocyanate and curcumin was found to be 50.29% in 36h and 89.15% in 150h respectively. The antioxidant activity of pure, benzylisothiocyanate, curcumin and cocktail (benzylisothiocyanate and curcumin) nanoemulsion was calculated with 2,2-diphenyl-1-picrylhydrazyl radical. The IC50value of different antioxidant showed that benzylisothiocyanate nanoemulsion acted as better antioxidant as compared to pure and curcumin encapsulated nanoemulsion. Also the cell viability of pure nanoemulsion was found to be 24% on hep G2 cell. The effect of UV light irradiation on curcumin and benzylisothiocyanate stability was carried out in different solvent conditions (water/ethanol and nanoemulsion). The degradation of curcumin by the impact of UV light was successfully controlled by trapping in NEm.
authors: Department of Chemistry and Centre for Advanced Studies in Chemistry, Panjab University, Chandigarh 160014, India. Electronic address: makkarkhushi@gmail.com.
authors: Khushwinder
authors: K
authors: Kaur
authors: Department of Chemistry and Centre for Advanced Studies in Chemistry, Panjab University, Chandigarh 160014, India.
authors: Jaspreet
authors: J
authors: Kaur
authors: Department of Chemistry and Centre for Advanced Studies in Chemistry, Panjab University, Chandigarh 160014, India.
authors: Raj
authors: R
authors: Kumar
authors: Department of Chemistry and Centre for Advanced Studies in Chemistry, Panjab University, Chandigarh 160014, India.
authors: S K
authors: SK
authors: Mehta
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D003474:Curcumin / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D065546:Drug Liberation; D004655:Emulsions; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017879:Isothiocyanates / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D009821:Oils / Q000737:chemistry*; D009994:Osmolar Concentration; D010316:Particle Size; D010851:Picrates / Q000737:chemistry; D011136:Polysorbates / Q000737:chemistry; D013010:Sonication; D013228:Stearates / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D014867:Water / Q000737:chemistry*; D024502:alpha-Tocopherol / Q000737:chemistry* / Q000633:toxicity
chemical_list: D001713:Biphenyl Compounds; D004337:Drug Carriers; D004655:Emulsions; D017879:Isothiocyanates; D009821:Oils; D010851:Picrates; D011136:Polysorbates; D013228:Stearates; D013501:Surface-Active Agents; D014867:Water; C031568:stearoyl-2-lactylic acid; C031403:benzyl isothiocyanate; C004931:1,1-diphenyl-2-picrylhydrazyl; D024502:alpha-Tocopherol; D003474:Curcumin
keywords: Anti proliferative; Antioxidant; Benzylisothiocyanate; Curcumin degradation; Nanoemulsions