title: Phenotypic and genotypic antimicrobial resistance and virulence genes ofSalmonella entericaisolated from pet dogs and cats.
abstract: Salmonella entericaisolates (n = 122), including 32 serotypes from 113 dogs and 9 cats, were obtained from household dogs (n = 250) and cats (n = 50) during 2012-2015. The isolates were characterized by serotyping, antimicrobial resistance phenotyping and genotyping, and virulence gene screening. Serovars Weltevreden (15.6%) and Typhimurium (13.9%) were the most common. The majority (43%) of the isolates were multidrug resistant. The dog isolates (12.3%) harbored class 1 integrons, of which thedfrA12-aadA2cassette was most frequent (66.7%). The only class integron in serovar Albany was located on a conjugative plasmid. Two ESBL-producing isolates (i.e., a serovar Krefeld and a serovar Enteritridis) carriedblaTEMandblaCTX-M, and theblaTEMgene in both was horizontally transferred. Of the plasmid-mediated quinolone resistance genes tested, onlyqnrS(4.9%) was detected. MostSalmonellaisolates harboredinvA(100%),prgH(91.8%), andsipB(91%). Positive associations between resistance and virulence genes were observed forblaPSE-1/orgA,cmlA/span,tolC, andsul1/tolC(p＜ 0.05). The results suggest that companion dogs and cats are potential sources ofS. entericastrains that carry resistance and virulence genes and that antimicrobial use in companion animals may select for the examinedSalmonellavirulence factors.
authors: Research Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Chulalongkorn University, Bangkok 10330, Thailand.|Center for Antimicrobial Resistance Monitoring in Foodborne Pathogens (in cooperation with WHO), Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Songsak
authors: S
authors: Srisanga
authors: Research Group for Prevention Technology in Livestock, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Sunpetch
authors: S
authors: Angkititrakul
authors: Research Group for Prevention Technology in Livestock, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Patcharee
authors: P
authors: Sringam
authors: Research Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Chulalongkorn University, Bangkok 10330, Thailand.|Department of Veterinary Biosciences, Faculty of Animal Sciences and Veterinary Medicine, Nong Lam University, Ho Chi Minh 70000, Vietnam.
authors: Phuong T
authors: PT
authors: Le Ho
authors: Department of Veterinary Biosciences, Faculty of Animal Sciences and Veterinary Medicine, Nong Lam University, Ho Chi Minh 70000, Vietnam.
authors: An T
authors: AT
authors: T Vo
authors: Research Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Chulalongkorn University, Bangkok 10330, Thailand.|Center for Antimicrobial Resistance Monitoring in Foodborne Pathogens (in cooperation with WHO), Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Rungtip
authors: R
authors: Chuanchuen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002371:Cat Diseases / Q000188:drug therapy / Q000382:microbiology; D002415:Cats; D004283:Dog Diseases / Q000188:drug therapy / Q000382:microbiology; D004285:Dogs; D024881:Drug Resistance, Bacterial / Q000235:genetics; D032023:Integrons / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012481:Salmonella Infections, Animal / Q000188:drug therapy / Q000382:microbiology*; D019779:Salmonella enterica / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D012703:Serotyping / Q000662:veterinary; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D037521:Virulence Factors
keywords: Salmonella enterica; antimicrobial resistance; integrons; pets; virulence factors