title: [Effect of Trichinella spiralis and its worm-derived proteins on CLP-induced sepsis in mice].
abstract: OBJECTIVE
  To observe the effect of Trichinella spiralis and its worm-derived proteins on cecal ligation and puncture (CLP)-induced sepsis in mice.
  
  
  METHODS
  Eighty male BALB/c mice were randomly divided into sham-operated group, CLP group, Trichinella spiralis muscle larvae (ML) pre-infection group (ML+CLP group), soluble muscle larvae proteins (SMP) treatment group (SMP+CLP group) and excretory-secretory proteins (MES) treatment group (MES+CLP group). In ML+CLP group, the mice were orally infected with 300 Trichinella spiralis muscle larvae at 28 days before CLP and those in the other groups were intraperitioneally injected with PBS or SMP (25 µg/mice) or MES (25µg/mice) 30 min after CLP. The general condition and 72-h survival after CLP of the mice were observed. The levels of alanine transaminase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), creatinine (Cr), TNF-α, IL-6, IL-1β, IL-10 and TGF-β were measured at 12 h after the operation, and the pathological changes of the liver and kidney were observed.
  
  
  RESULTS
  s Compared with the sham-operated mice, the mice in CLP group showed decreased 72-h survival, obviously increased ALT, AST, BUN, Cr, TNF-α, IL-6, IL-1β, IL-10, and TGF-β with hepatic cords disorder, hepatocytes swelling, glomerulus shrinkage, and renal tubular cell edema. Compared with CLP group, the mice in ML+CLP group showed lowered levels of ALT, AST, Cr, TNF-α and IL-1β and increased levels of IL-10 and TGF-β; in SMP+CLP group, the levels of ALT, AST, Cr, TNF-α and IL-1β were decreased and TGF-β increased. In MES+CLP group, the mice showed obviously increased 72-h survival with lowered levels of ALT, AST, BUN, Cr, TNF-α, IL-6 and IL-1β, increased levels of IL-10 and TGF-β, and alleviated liver and kidney damages.
  
  
  CONCLUSIONS
  Trichinella spiralis and its worm-derived proteins can decrease the levels of pro-inflammatory cytokines and increase immunomodulatory cytokines, and MES has more potent effect to reduce structural and functional damages of the liver and kidney.
authors: Department of Histology and Embryology, Bengbu Medical College, Bengbu 233000, China. E-mail: dearlxh@163.com.
authors: Hui-Hui
authors: HH
authors: Li
authors: Wen-Xin
authors: WX
authors: He
authors: Di
authors: D
authors: Song
authors: Qi
authors: Q
authors: Wu
authors: Nan
authors: N
authors: Li
authors: Yong-Kun
authors: YK
authors: Wan
authors: Hui
authors: H
authors: Zhang
authors: Da-Peng
authors: DP
authors: Qiu
authors: Liang
authors: L
authors: Chu
authors: Li-Yuan
authors: LY
authors: Wang
authors: Xiao-di
authors: XD
authors: Yang
authors: Qiang
authors: Q
authors: Fang
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000947:Antigens, Helminth / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D001806:Blood Urea Nitrogen; D002432:Cecum; D003404:Creatinine / Q000097:blood; D016207:Cytokines / Q000097:blood; D015801:Helminth Proteins / Q000494:pharmacology*; D007668:Kidney / Q000503:physiopathology; D007674:Kidney Diseases / Q000628:therapy*; D008026:Ligation; D008099:Liver / Q000503:physiopathology; D008107:Liver Diseases / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018805:Sepsis / Q000628:therapy*; D017160:Trichinella spiralis
chemical_list: D000947:Antigens, Helminth; D016207:Cytokines; D015801:Helminth Proteins; C097419:excretory-secretory antigen, Trichinella; D003404:Creatinine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase