title: Analysis of multitemperature isothermal titration calorimetry data at very low c: Global beats van't Hoff.
abstract: Isothermal titration calorimetry data for very low c (≡K[M]0) must normally be analyzed with the stoichiometry parameter n fixed - at its known value or at any reasonable value if the system is not well characterized. In the latter case, ΔH° (and hence n) can be estimated from the T-dependence of the binding constant K, using the van't Hoff (vH) relation. An alternative is global or simultaneous fitting of data at multiple temperatures. In this Note, global analysis of low-c data at two temperatures is shown to estimate ΔH° and n with double the precision of the vH method.
authors: Department of Chemistry, Vanderbilt University, Nashville, TN 37235, United States. Electronic address: joel.tellinghuisen@vanderbilt.edu.
authors: Joel
authors: J
authors: Tellinghuisen
mesh_terms: D002153:Calorimetry, Indirect / Q000379:methods; D008962:Models, Theoretical
keywords: Data analysis; Global analysis; ITC; Nonlinear least squares; Statistical errors