title: Establishing a tree shrew model of systemic lupus erythematosus and cell transplantation treatment.
abstract: The establishment of a tree shrew model for systemic lupus erythematosus (SLE) provides a new method to evaluate the pathogenesis of autoimmune diseases.
  
  
  
  Eighty tree shrews were randomly divided into four groups receiving either an intraperitoneal injection of pristane, lipopolysaccharide (LPS), or pristane and LPS, or no injection. Three weeks after injection, the SLE model tree shrews were divided into the model group and the treatment group. Tree shrews in the treatment group and the normal control group were infused with umbilical cord mesenchymal stem cells (UC-MSCs). The cells were labeled with DiR. Two weeks after transplantation, three groups of tree shrews were analyzed for urine protein, serum antinuclear antibodies and antiphospholipid, and inflammatory cytokine antibody microarray detection. The heart, liver, spleen, lung, and kidney were collected from the three groups and subjected to hematoxylin and eosin (HE) staining and detection of renal immune complex deposition.
  
  
  
  HE staining indicated pathology in the model group. Red fluorescence revealed immune complex deposition in the kidneys from the model group.
  
  
  
  The combined intraperitoneal injection of pristane and LPS is the best way to induce SLE pathological changes. The pathological changes improved after UC-MSC treatment.
authors: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, People's Republic of China. ruangp@126.com.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, People's Republic of China. ruangp@126.com.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, People's Republic of China. ruangp@126.com.
authors: Guang-Ping
authors: GP
authors: Ruan
authors: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, People's Republic of China.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, People's Republic of China.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, People's Republic of China.
authors: Xiang
authors: X
authors: Yao
authors: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, People's Republic of China.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, People's Republic of China.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, People's Republic of China.
authors: Ju-Fen
authors: JF
authors: Liu
authors: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, People's Republic of China.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, People's Republic of China.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, People's Republic of China.
authors: Jie
authors: J
authors: He
authors: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, People's Republic of China.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, People's Republic of China.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, People's Republic of China.
authors: Zi-An
authors: ZA
authors: Li
authors: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, People's Republic of China.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, People's Republic of China.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, People's Republic of China.
authors: Jian-Yong
authors: JY
authors: Yang
authors: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, People's Republic of China.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, People's Republic of China.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, People's Republic of China.
authors: Rong-Qing
authors: RQ
authors: Pang
authors: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, People's Republic of China. ynkmry@163.com.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, People's Republic of China. ynkmry@163.com.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, People's Republic of China. ynkmry@163.com.
authors: Xing-Hua
authors: XH
authors: Pan
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008180:Lupus Erythematosus, Systemic / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology* / Q000628:therapy*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000473:pathology; D013729:Terpenes / Q000494:pharmacology; D014418:Tupaiidae; D014470:Umbilical Cord / Q000187:drug effects / Q000473:pathology
chemical_list: D008070:Lipopolysaccharides; D013729:Terpenes; C009042:pristane
keywords: Animal models; Systemic lupus erythematosus; Transplantation; Tree shrews; Umbilical cord mesenchymal stem cells