title: Mining potential biomarkers associated with space flight in Caenorhabditis elegans experienced Shenzhou-8 mission with multiple feature selection techniques.
abstract: To identify the potential biomarkers associated with space flight, a combined algorithm, which integrates the feature selection techniques, was used to deal with the microarray datasets of Caenorhabditis elegans obtained in the Shenzhou-8 mission. Compared with the ground control treatment, a total of 86 differentially expressed (DE) genes in responses to space synthetic environment or space radiation environment were identified by two filter methods. And then the top 30 ranking genes were selected by the random forest algorithm. Gene Ontology annotation and functional enrichment analyses showed that these genes were mainly associated with metabolism process. Furthermore, clustering analysis showed that 17 genes among these are positive, including 9 for space synthetic environment and 8 for space radiation environment only. These genes could be used as the biomarkers to reflect the space environment stresses. In addition, we also found that microgravity is the main stress factor to change the expression patterns of biomarkers for the short-duration spaceflight.
authors: Institute of Environmental Systems Biology, College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, People's Republic of China.
authors: Lei
authors: L
authors: Zhao
authors: Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Shushanhu Road 350, Hefei 230031, People's Republic of China.
authors: Ying
authors: Y
authors: Gao
authors: Department of Physics, Dalian Maritime University, Dalian 116026, People's Republic of China. Electronic address: mid@dlmu.edu.cn.
authors: Dong
authors: D
authors: Mi
authors: Institute of Environmental Systems Biology, College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, People's Republic of China. Electronic address: yqsun@dlmu.edu.cn.
authors: Yeqing
authors: Y
authors: Sun
mesh_terms: D000465:Algorithms; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D017173:Caenorhabditis elegans / Q000235:genetics / Q000528:radiation effects; D016000:Cluster Analysis; D003359:Cosmic Radiation / Q000009:adverse effects; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000528:radiation effects; D063990:Gene Ontology; D007814:Larva; D046228:Microarray Analysis / Q000379:methods*; D013026:Space Flight; D059467:Transcriptome / Q000528:radiation effects; D014893:Weightlessness / Q000009:adverse effects
chemical_list: D015415:Biomarkers
keywords: Biomarkers; Caenorhabditis elegans; Feature selection; Microarray; Space radiation; Spaceflight