title: Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor.
abstract: OBJECTIVE
  To study the effects of Prunella vulgaris polysaccharide (PVP) on human breast carcinoma-associated fibroblasts (CAFs).
  
  
  METHODS
  Cell viability was detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-(3-carboxymethoxyphenyl)-2-4-sulfophenyl)-2H-tetrazolium (MTS) assay. Wound healing experiment and transwell migration assay were used to investigate the anti-migration effects. Flow cytometry was applied to detect cell apoptosis and cell cycle distribution. Reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect the expression of basic fibroblast growth factor (bFGF) in CAFs. Culture SKBr-3 with CAFs conditioned medium (CAFs-CM) to evaluate the indirect function on the proliferation of breast cancer SKBr-3 cells.
  
  
  RESULTS
  PVP inhibited the viability of CAFs by inducing apoptosis (P <0.01) and arresting cell cycle (P <0.01). It also inhibited the migration of CAFs (P <0.01). bFGF promoted CAFs proliferation (P <0.01) and migration (P <0.01), protected CAFs from apoptosis (P <0.05) and reduced G0phase to 49.06% (P <0.01). However, these effects of bFGF on CAFs could be abrogated by PVP. Culturing SKBr-3 with CAFs-CM, PVP could inhibit the viability of breast cancer SKBr-3 cells indirectly. Moreover, PVP reduced the mRNA expression (P <0.01) and protein secretion of bFGF (P <0.01) in CAFs.
  
  
  CONCLUSIONS
  PVP could exert an anti-cancer effect on breast CAFs by inhibiting bFGF expression, thus inhibiting the growth of breast cancer SKBr-3 cells indirectly.
authors: Zhong-Shan-Men In-Patient Department, Tianjin Medical University Cancer Institute and Hospital; National Clinical Research Center for Cancer; Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.|Clincal Center of Tumor Therapy, The Fourth Central Hospital Affiliated to Nankai University, Tianjin, 300140, China.
authors: Jian
authors: J
authors: Hao
authors: Zhong-Shan-Men In-Patient Department, Tianjin Medical University Cancer Institute and Hospital; National Clinical Research Center for Cancer; Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
authors: Xiu-Li
authors: XL
authors: Ding
authors: Zhong-Shan-Men In-Patient Department, Tianjin Medical University Cancer Institute and Hospital; National Clinical Research Center for Cancer; Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
authors: Xue
authors: X
authors: Yang
authors: Zhong-Shan-Men In-Patient Department, Tianjin Medical University Cancer Institute and Hospital; National Clinical Research Center for Cancer; Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China. wuxiongzhi@163.com.
authors: Xiong-Zhi
authors: XZ
authors: Wu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017077:Culture Media, Conditioned; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D031347:Prunella
chemical_list: C518724:CML-1 plant extract; D017077:Culture Media, Conditioned; D010936:Plant Extracts; D011134:Polysaccharides; D016222:Fibroblast Growth Factor 2
keywords: Chinese medicine; Prunella vulgaris polysaccharide; SKBr-3; basic fibroblast growth factor; carcinoma-associated fibroblasts