title: Simplified Enzymatic Upgrading of High-Acid Rice Bran Oil Using Ethanol as a Novel Acyl Acceptor.
abstract: One of the major challenges in the upgrading of high-acid rice bran oil (RBO) is to efficiently reduce the amount of free fatty acids. Here we report a novel method for upgrading high-acid RBO using ethanol as a novel acyl acceptor in combination with a highly selective lipase from Malassezia globosa (SMG1-F278N). This process enabled an unprecedented deacidification efficiency of up to 99.80% in a short time (6 h); the immobilized SMG1-F278N used in deacidification exhibited excellent operational stability and could be used for at least 10 consecutive batches without detectable loss in activity. Scale-up was performed under optimized conditions to verify the applicability of this process, and low-acid (0.08%) RBO with a high level of γ-oryzanol (27.8 g/kg) and γ-oryzanol accumulation fold (1.5) was obtained after molecular distillation at lower temperature (120 °C). Overall, we report a simplified and efficient procedure for the production of edible RBO from high-acid RBO.
authors: School of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
authors: Daoming
authors: D
authors: Li
authors: School of Chemistry and Chemical Engineering, South China University of Technology , Guangzhou 510640, China.
authors: Weifei
authors: W
authors: Wang
authors: School of Bioscience and Bioengineering, South China University of Technology , Guangzhou 510006, China.
authors: Rabia
authors: R
authors: Durrani
authors: School of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
authors: Xingxing
authors: X
authors: Li
authors: School of Bioscience and Bioengineering, South China University of Technology , Guangzhou 510006, China.
authors: Bo
authors: B
authors: Yang
authors: School of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
authors: Yonghua
authors: Y
authors: Wang
mesh_terms: D055162:Biocatalysis; D000431:Ethanol / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D008290:Malassezia / Q000201:enzymology*; D010938:Plant Oils / Q000737:chemistry*; D000073879:Rice Bran Oil
chemical_list: D005656:Fungal Proteins; D010938:Plant Oils; D000431:Ethanol; D008049:Lipase; D000073879:Rice Bran Oil
keywords: deacidification; high-acid rice bran oil; monoacylglycerol and diacylglycerol lipase; upgrading; γ-oryzanol