title: Quantitative Proteomics Reveals the Mechanism of Oxygen Treatment on Lenses of Alzheimer's Disease Model Mice.
abstract: Alzheimer's disease (AD) is a neurodegenerative disease with well-characterized pathological features. Yet the underlying mechanisms have not been resolved and an effective therapeutic approach is lacking. Cerebral hypoxia is considered a risk factor of AD.
  
  
  
  We tested whether oxygen supplementation can relieve AD symptoms and how it affects the expression levels of proteins in the lens.
  
  
  
  Triple transgenic AD model (3xTg-AD) mice were divided into oxygen treated (OT) and control (Ctrl) groups. Their cognitive performances were tested in a Morris water maze (MWM) paradigm. Then, their eye lens tissues were subjected to quantitative proteomics analysis by the iTRAQ (isobaric tags for relative and absolute quantification) method. The up- and downregulated proteins were classified according to a Gene Ontology (GO) database in PANTHER. Behavioral and proteomic data were compared between the groups.
  
  
  
  Mice in the OT group had better learning and memorizing performance compared with the Ctrl group in MWM test. Lenses from the OT group had 205 differentially regulated proteins, relative to lenses from the Ctrl group, including proteins that are involved in the clearance of amyloid β-protein.
  
  
  
  The results of this study indicate that oxygen treatment can improve cognitive function in AD model mice and alters protein expression in a manner consistent with improved redox regulation.
authors: College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, China.
authors: Hao
authors: H
authors: Wang
authors: College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, China.
authors: Ying
authors: Y
authors: Wang
authors: College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, China.
authors: Xiaoyu
authors: X
authors: Hong
authors: College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, Shenzhen, China.
authors: Shuiming
authors: S
authors: Li
authors: College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, Shenzhen, China.
authors: Yong
authors: Y
authors: Wang
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism* / Q000628:therapy*; D000818:Animals; D004195:Disease Models, Animal; D007908:Lens, Crystalline / Q000378:metabolism*; D018782:Maze Learning; D008822:Mice, Transgenic; D010100:Oxygen / Q000627:therapeutic use*; D020543:Proteome; D040901:Proteomics
chemical_list: D020543:Proteome; D010100:Oxygen
keywords: Alzheimer’s disease; iTRAQ; mass spectrometry; oxygen; proteomics; water maze