title: Effects of PTEN gene silencing on invasion and EMT in oral squamous carcinoma Tca8113 cells.
abstract: OBJECTIVE
  To investigate the impact of silencing of the PTEN gene using siRNA on the invasion, proliferation, cell cycle, and epithelial-mesenchymal transition of the Tca8113 cell line.
  
  
  METHODS
  The established Tca8113 cell model with siRNA interference to silence the PTEN gene was used. The transfection efficiency was examined by RT-qPCR and Western blot analysis. CCK-8 assay was utilized to analyze the proliferation of Tca8113 cells and cell invasion was evaluated using a transwell assay. The cell cycle distribution was analyzed by flow cytometry. The protein expression levels of the epithelial-mesenchymal transition (EMT) markers E-cadherin and Vimentin and the EMT-related proteins β-catenin and TGF-β1 were analyzed by Western blot.
  
  
  RESULTS
  The expression level of PTEN was significantly reduced in the PTEN-siRNA group. The invasiveness and proliferation rate of Tca8113 cells in the PTEN-siRNA group were significantly greater than those of the control and negative control groups. The expression levels of E-cadherin and β-catenin were reduced, whereas the expression levels of vimentin and TGFβ-1 were elevated in the PTEN-siRNA group compared with those of control and negative groups. These results were significantly different.
  
  
  CONCLUSIONS
  The silencing of PTEN by siRNA increased the proliferation and promoted cell invasion of Tca8113 cells. PTEN gene silencing may accelerate the EMT in Tca8113 cells.
authors: Department of Oral Pathology, Medicine School, Jinan University, Guangzhou, China.|Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Zhiyuan
authors: Z
authors: Lu
authors: Clinical Medicine Postdoctoral Mobile Station, Jinan University, Guangzhou, China.
authors: Hui
authors: H
authors: Guo
authors: Department of Oral Pathology, Medicine School, Jinan University, Guangzhou, China.
authors: Yanzhu
authors: Y
authors: Lin
authors: Department of Oral Pathology, Medicine School, Jinan University, Guangzhou, China.
authors: Lijia
authors: L
authors: Shen
authors: Department Of Oral Medicine, Guangdong Provincial Stomatological Hospital & the Affiliated Stomatological Hospital of Southern Medical University, Guangzhou, China.
authors: Cao
authors: C
authors: Yin
authors: Department of Oral Pathology, Medicine School, Jinan University, Guangzhou, China.|Guangdong Province Key Laboratory of Molecule Immunology and Antibody Engineering, Jinan University, Guangzhou, China.
authors: Siming
authors: S
authors: Xie
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D020868:Gene Silencing; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000235:genetics; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000502:physiology; D034741:RNA, Small Interfering; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D034741:RNA, Small Interfering; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D051176:beta Catenin; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: 
  EMT
  ; 
  PTEN
  ; Tca8113 cells; invasion; small interference RNA