title: Molecular performance of Prl and Gh/Igf1 axis in the Mediterranean meager, Argyrosomus regius, acclimated to different rearing salinities.
abstract: Aquaculture industry in the Mediterranean region exhibits a growing interest for the Mediterranean meager Argyrosomus regius. Some preliminary works showed a good growth performance of the species in nearly isosmotic salinities. However, the patterns of alteration of prolactin (Prl) as well as growth hormone (Gh)/insulin growth factor-1 (Igf1) axis at the molecular level are not yet described in this species. Therefore, we cloned and sequenced partial cDNAs for pituitary prolactin (prl) and growth hormone (gh), hepatic insulin-like growth factor (igf1), and β-actin (actb). Expression patterns of these transcripts were tested in juveniles of A. regius acclimated to four different environmental salinities: (1) 5 ‰ (hyposmotic); (2) 12 ‰ (isosmotic); (3) 38 ‰ (hyperosmotic; seawater control); and (4) 55 ‰ (extremely hyperosmotic). All investigated transcripts shared high sequence identities with their counterparts in other perciformes. prl mRNA levels showed inverse pattern with increasing salinities. gh mRNA enhanced significantly in both 12 and 55 ‰ salinity groups in comparison with the control group, while igf1 showed its maximum expression levels under the nearly isosmotic environment. The results indicated clear sensitivity of prl, gh and igf1 to changes in environmental salinity, which can possibly control the euryhalinity capacity of this species.
authors: Genetic Engineering and Molecular Biology Division, Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Menoufia, Egypt. khaled.mohammed@icman.csic.es.|Instituto de Ciencias Marinas de Andalucía, Consejo Superior de Investigaciones Científicas (ICMAN-CSIC), 11519, Puerto Real, Cádiz, Spain. khaled.mohammed@icman.csic.es.|Department of Biology, Faculty of Marine and Environmental Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cadiz, 11519, Puerto Real, Cadiz, Spain. khaled.mohammed@icman.csic.es.
authors: Khaled
authors: K
authors: Mohammed-Geba
authors: Department of Biomedicine, Biotechnology, and Public Health, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cadiz, 11519, Puerto Real, Cádiz, Spain.
authors: Antonio Astola
authors: AA
authors: González
authors: Department of Biomedicine, Biotechnology, and Public Health, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cadiz, 11519, Puerto Real, Cádiz, Spain.
authors: Rubén Ayala
authors: RA
authors: Suárez
authors: Genetic Engineering and Molecular Biology Division, Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Menoufia, Egypt.|Instituto de Ciencias Marinas de Andalucía, Consejo Superior de Investigaciones Científicas (ICMAN-CSIC), 11519, Puerto Real, Cádiz, Spain.|Department of Biology, Faculty of Marine and Environmental Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cadiz, 11519, Puerto Real, Cadiz, Spain.
authors: Asmaa
authors: A
authors: Galal-Khallaf
authors: Instituto de Ciencias Marinas de Andalucía, Consejo Superior de Investigaciones Científicas (ICMAN-CSIC), 11519, Puerto Real, Cádiz, Spain.
authors: Juan Antonio
authors: JA
authors: Martos-Sitcha
authors: Immunology and Parasitology Division, Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Menoufia, Egypt.
authors: Hany Mohammed
authors: HM
authors: Ibrahim
authors: Instituto de Ciencias Marinas de Andalucía, Consejo Superior de Investigaciones Científicas (ICMAN-CSIC), 11519, Puerto Real, Cádiz, Spain.
authors: Gonzalo
authors: G
authors: Martínez-Rodríguez
authors: Department of Biology, Faculty of Marine and Environmental Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cadiz, 11519, Puerto Real, Cadiz, Spain.
authors: Juan Miguel
authors: JM
authors: Mancera
mesh_terms: D000064:Acclimatization / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics*; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D010473:Perciformes / Q000235:genetics*; D010802:Phylogeny; D011388:Prolactin / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D054712:Salinity
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D011388:Prolactin; D013006:Growth Hormone
keywords: Argyrosomus regius; Euryhaline; Salinity; actb; gh; igf1; prl; qPCR