title: Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos.
abstract: High-throughput screening with a loss-of-function strategy is a logical and efficient way to identify novel genes involved in biological processes of interest. In zebrafish, morpholinos have been developed as a convenient tool to knock down gene expression. Here, we describe procedures for systematic screening using morpholinos in zebrafish to identify novel deubiquitylases involved in convergent extension during gastrulation. In this example, we examine candidates based on embryonic morphology and molecular signals of whole mount in situ hybridization assay.
authors: Institute of Molecular and Genomic Medicine, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan.
authors: Li-Chuan
authors: LC
authors: Tseng
authors: Institute of Molecular and Genomic Medicine, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan.
authors: Chih-Hao
authors: CH
authors: Tang
authors: Institute of Molecular and Genomic Medicine, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan. yjjiang@nhri.org.tw.
authors: Yun-Jin
authors: YJ
authors: Jiang
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian; D054262:Gastrulation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques / Q000379:methods; D057166:High-Throughput Screening Assays / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D008845:Microinjections / Q000379:methods; D060172:Morpholinos / Q000235:genetics* / Q000494:pharmacology; D064570:Ubiquitin-Specific Proteases / Q000235:genetics; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D060172:Morpholinos; D029961:Zebrafish Proteins; D064570:Ubiquitin-Specific Proteases
keywords: In situ hybridization; Knockdown; Morpholinos; RNA probe; Zebrafish