title: Bacillus subtilisfrom Soybean Food Shows Antimicrobial Activity for Multidrug-ResistantAcinetobacter baumanniiby Affecting theadeSGene.
abstract: Exploring novel antibiotics is necessary for multidrug-resistant pathogenic bacteria. Because the probiotics in soybean food have antimicrobial activities, we investigated their effects on multidrug-resistantAcinetobacter baumannii. Nineteen multidrug-resistantA. baumanniistrains were clinifcally isolated as an experimental group and 11 multidrug-sensitive strains as controls. The growth rates of all bacteria were determined by using the analysis for xCELLigence Real-Time Cell. The combination of antibiotics showed synergistic effects on the strains in the control group but no effect on the strains in the experimental group. Efflux pump geneadeSwas absent in all the strains from the control group, whereas it exists in all the strains from the experimental group. Furthermore, all the strains lost multidrug resistance when anadeSinhibitor was used. One strain of probiotics isolated from soybean food showed high antimicrobial activity for multidrug-resistantA. baumannii. The isolated strain belongs toBacillus subtilisaccording to 16S RNA analysis. Furthermore,E. colishowed multidrug resistance when it was transformed with theadeSgene fromA. baumanniiwhereas the resistant bacteria could be inhibited completely by isolatedBacillus subtilis. Thus, probiotics from soybean food provide potential antibiotics against multidrug-resistant pathogenic bacteria.
authors: Clinical Laboratory Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors: Tieshan
authors: T
authors: Wang
authors: Clinical Laboratory Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors: Jianrong
authors: J
authors: Su
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000898:Antibiosis; D001412:Bacillus subtilis / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial; D015964:Gene Expression Regulation, Bacterial; D045730:Soy Foods / Q000382:microbiology*; D013025:Glycine max / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins
keywords: Acinetobacter baumannii; Probiotics; adeS efflux pump; antibiotics; multidrug resistance; soy curd