title: Presynaptic GABA Receptors Mediate Temporal Contrast Enhancement in Drosophila Olfactory Sensory Neurons and Modulate Odor-Driven Behavioral Kinetics.
abstract: Contrast enhancement mediated by lateral inhibition within the nervous system enhances the detection of salient features of visual and auditory stimuli, such as spatial and temporal edges. However, it remains unclear how mechanisms for temporal contrast enhancement in the olfactory system can enhance the detection of odor plume edges during navigation. To address this question, we delivered to Drosophila melanogaster flies pulses of high odor intensity that induce sustained peripheral responses in olfactory sensory neurons (OSNs). We use optical electrophysiology to directly measure electrical responses in presynaptic terminals and demonstrate that sustained peripheral responses are temporally sharpened by the combined activity of two types of inhibitory GABA receptors to generate contrast-enhanced voltage responses in central OSN axon terminals. Furthermore, we show how these GABA receptors modulate the time course of innate behavioral responses after odor pulse termination, demonstrating an important role for temporal contrast enhancement in odor-guided navigation.
authors: Department of Cellular and Molecular Physiology, Yale University School of Medicine , New Haven, Connecticut 06520.
authors: Davide
authors: 0000-0003-1999-1505
authors: D
authors: Raccuglia
authors: Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520; Interdepartmental Neuroscience Program, Yale University, New Haven, Connecticut 06520.
authors: Li Yan
authors: 0000-0002-8862-6715
authors: LY
authors: McCurdy
authors: Department of Molecular, Cellular and Developmental Biology, Yale University , New Haven, Connecticut 06520.
authors: Mahmut
authors: M
authors: Demir
authors: Interdepartmental Neuroscience Program, Yale University , New Haven, Connecticut 06520.
authors: Srinivas
authors: S
authors: Gorur-Shandilya
authors: Department of Cellular and Molecular Physiology, Yale University School of Medicine , New Haven, Connecticut 06520.
authors: Michael
authors: M
authors: Kunst
authors: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520; Department of Physics, Yale University, New Haven, Connecticut 06520.
authors: Thierry
authors: 0000-0002-6746-6564
authors: T
authors: Emonet
authors: Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520; Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520; Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, Connecticut 06520.
authors: Michael N
authors: MN
authors: Nitabach
mesh_terms: D000200:Action Potentials; D000818:Animals; D030801:Animals, Genetically Modified; D001362:Avoidance Learning; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster; D005260:Female; D018682:GABA Agents / Q000494:pharmacology; D009043:Motor Activity; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000378:metabolism*; D061848:Optical Imaging; D017729:Presynaptic Terminals / Q000187:drug effects / Q000378:metabolism*; D018079:Receptors, GABA / Q000235:genetics / Q000378:metabolism*; D018035:Receptors, Odorant / Q000378:metabolism; D012903:Smell / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
chemical_list: D029721:Drosophila Proteins; D018682:GABA Agents; C496847:OR22a protein, Drosophila; D018079:Receptors, GABA; D018035:Receptors, Odorant