title: Toxicity study of ionic liquid, 1-butyl-3-methylimidazolium bromide on guppy fish, Poecilia reticulata and its biodegradation by soil bacterium Rhodococcus hoagii VRT1.
abstract: This study deals with the toxic effect of ionic liquid, 1-butyl-3-methylimidazolium bromide (BMImBr) on guppy fish, Poecilia reticulata. The fishes were exposed to various concentrations of ionic liquid for 96h. The activity of antioxidant enzymes viz. catalase, glutathione S-transferase and superoxide dismutase were found to be increased with increase in concentration. The BMImBr resistant bacterium were isolated from garden soil by enrichment method and identified as Rhodococcus hoagii VRT1 by 16S rDNA sequencing. An isolated bacterium was effective in biodegradation of compound in 8 days which was analyzed by changes in BOD and COD and later on confirmed by HRMS analysis. Higher concentrations of compound induced DNA damage in liver cells while degraded product did not show adverse impact on the DNA integrity.
authors: Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University, Pune 411007, India.
authors: Viresh R
authors: VR
authors: Thamke
authors: Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University, Pune 411007, India. Electronic address: kodam@chem.unipune.ac.in.
authors: Kisan M
authors: KM
authors: Kodam
mesh_terms: D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000818:Animals; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D002374:Catalase / Q000378:metabolism; D020552:Comet Assay; D005978:Glutathione / Q000378:metabolism; D007093:Imidazoles / Q000378:metabolism / Q000633:toxicity*; D052578:Ionic Liquids / Q000378:metabolism / Q000633:toxicity*; D011036:Poecilia; D012240:Rhodococcus / Q000302:isolation & purification / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D007093:Imidazoles; D052578:Ionic Liquids; C502841:1-butyl-3-methylimidazolium chloride; D002374:Catalase; D013482:Superoxide Dismutase; D000633:Aminopyrine N-Demethylase; D005978:Glutathione
keywords: Antioxidant enzymes; Biodegradation; Genotoxicity; Guppy fish; Ionic liquid