title: TSC22D2 interacts with PKM2 and inhibits cell growth in colorectal cancer.
abstract: We previously identified TSC22D2 (transforming growth factor β-stimulated clone 22 domain family, member 2) as a novel cancer-associated gene in a rare multi-cancer family. However, its role in tumor development remains completely unknown. In this study, we found that TSC22D2 was significantly downregulated in colorectal cancer (CRC) and that TSC22D2 overexpression inhibited cell growth. Using a co-immunoprecipitation (co-IP) assay combined with mass spectrometry analysis to identify TSC22D2-interacting proteins, we demonstrated that TSC22D2 interacts with pyruvate kinase isoform M2 (PKM2). These findings were confirmed by the results of immunoprecipitation and immunofluorescence assays. Moreover, overexpression of TSC22D2 reduced the level of nuclear PKM2 and suppressed cyclin D1 expression. Collectively, our study reveals a growth suppressor function of TSC22D2 that is at least partially dependent on the TSC22D2-PKM2-cyclinD1 regulatory axis. In addition, our data provide important clues that might contribute to future studies evaluating the role of TSC22D2.
authors: Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Cancer Research Institute, Central South University, Changsha, Hunan, P.R. China.
authors: Fang
authors: F
authors: Liang
authors: Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Cancer Research Institute, Central South University, Changsha, Hunan, P.R. China.
authors: Qiao
authors: Q
authors: Li
authors: Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China.
authors: Xiayu
authors: X
authors: Li
authors: Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Cancer Research Institute, Central South University, Changsha, Hunan, P.R. China.
authors: Zheng
authors: Z
authors: Li
authors: The Key Laboratory of Carcinogenesis of The Chinese Ministry of Health and The Key Laboratory of Carcinogenesis and Cancer Invasion of The Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, P.R. China.
authors: Zhaojian
authors: Z
authors: Gong
authors: Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China.
authors: Hao
authors: H
authors: Deng
authors: Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Cancer Research Institute, Central South University, Changsha, Hunan, P.R. China.
authors: Bo
authors: B
authors: Xiang
authors: Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Cancer Research Institute, Central South University, Changsha, Hunan, P.R. China.
authors: Ming
authors: M
authors: Zhou
authors: Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Cancer Research Institute, Central South University, Changsha, Hunan, P.R. China.
authors: Xiaoling
authors: X
authors: Li
authors: Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Cancer Research Institute, Central South University, Changsha, Hunan, P.R. China.
authors: Guiyuan
authors: G
authors: Li
authors: Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Cancer Research Institute, Central South University, Changsha, Hunan, P.R. China.
authors: Zhaoyang
authors: Z
authors: Zeng
authors: Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Cancer Research Institute, Central South University, Changsha, Hunan, P.R. China.
authors: Wei
authors: W
authors: Xiong
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism*; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D013963:Thyroid Hormones / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors; D000097810:Thyroid Hormone-Binding Proteins
chemical_list: D002352:Carrier Proteins; D019938:Cyclin D1; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D013963:Thyroid Hormones; D014157:Transcription Factors; D000097810:Thyroid Hormone-Binding Proteins; C530104:CCND1 protein, human; C574229:TSC22D2 protein, human