title: Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes.
abstract: The adoptive transfer of a bolus of tumor-specific T lymphocytes into cancer patients is a promising therapeutic strategy. In one approach, tumor specificity is conferred upon T cells via engineering expression of exogenous receptors, such as chimeric antigen receptors (CARs). Here, we describe the generation and production of both murine and human CAR-engineered T lymphocytes using retroviruses.
authors: Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University, 1200 Main Street West, Hamilton, ON, Canada, L8N 3Z5.
authors: Joanne A
authors: JA
authors: Hammill
authors: Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University, 1200 Main Street West, Hamilton, ON, Canada, L8N 3Z5.
authors: Arya
authors: A
authors: Afsahi
authors: Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University, 1200 Main Street West, Hamilton, ON, Canada, L8N 3Z5. bramsonj@mcmaster.ca.
authors: Jonathan L
authors: JL
authors: Bramson
authors: Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University, 1200 Main Street West, Hamilton, ON, Canada, L8N 3Z5.
authors: Christopher W
authors: CW
authors: Helsen
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D018014:Gene Transfer Techniques; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D016219:Immunotherapy, Adoptive; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D009369:Neoplasms / Q000276:immunology / Q000628:therapy; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D014161:Transduction, Genetic
chemical_list: D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins
keywords: CAR-T cell; Chimeric antigen receptor; Gamma-retrovirus; Lentivirus; T lymphocytes