title: Kamishoyosan reduces conditioned fear-induced freezing behavior in socially isolated ovariectomized rats.
abstract: In the present study, we investigated the effect of kamishoyosan (KSS) on conditioned fear-induced freezing in ovariectomized (OVX) rats. Socially isolated OVX rats showed the longest freezing time among the following four groups: group-housed sham-operated (Sham), isolated Sham, group-housed OVX, and isolated OVX rats. Repeated oral administration of KSS (30-300 mg/kg) reduced conditioned fear-induced freezing in socially isolated OVX rats. The reduction of freezing by KSS was reversed by flumazenil (3 mg/kg) and bicuculline (3 mg/kg). These findings suggest that the GABAA-benzodiazepine receptor complex is involved in the anxiolytic effect of KSS in socially isolated OVX rats.
authors: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan; Department of Pharmacy, Kyushu University Hospital, Fukuoka 812-8582, Japan. Electronic address: n-egashi@pharm.med.kyushu-u.ac.jp.
authors: Nobuaki
authors: N
authors: Egashira
authors: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan.
authors: Hikari
authors: H
authors: Iba
authors: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan.
authors: Haruna
authors: H
authors: Kuwano
authors: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan.
authors: Rikako
authors: R
authors: Kawanaka
authors: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan.
authors: Masaki
authors: M
authors: Nagao
authors: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan.
authors: Hiroshi
authors: H
authors: Moriyama
authors: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan.
authors: Takuya
authors: T
authors: Watanabe
authors: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan; A.I.G. Collaborative Research Institute for Aging and Brain Sciences, Fukuoka University, Fukuoka 814-0180, Japan.
authors: Kaori
authors: K
authors: Kubota
authors: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan.
authors: Shutaro
authors: S
authors: Katsurabayashi
authors: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan; A.I.G. Collaborative Research Institute for Aging and Brain Sciences, Fukuoka University, Fukuoka 814-0180, Japan.
authors: Katsunori
authors: K
authors: Iwasaki
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D003213:Conditioning, Psychological / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005239:Fear / Q000187:drug effects*; D005260:Female; D005442:Flumazenil / Q000494:pharmacology; D041743:Herb-Drug Interactions; D046353:Immobility Response, Tonic / Q000187:drug effects*; D010052:Ovariectomy; D051381:Rats; D012934:Social Isolation
chemical_list: D004365:Drugs, Chinese Herbal; C103354:kamisyoyo san; D005442:Flumazenil; D001640:Bicuculline
keywords: Conditioned fear stress; Kamishoyosan; Ovariectomized rats