title: Isolation and Proteomic Analysis of aChlamydomonas reinhardtiiMutant with Enhanced Lipid Production by the Gamma Irradiation Method.
abstract: In this study, an enhanced lipid-producing mutant strain of the microalgaChlamydomonas reinhardtiiwas developed by gamma irradiation. To induce the mutation,C. reinhardtiiwas gamma irradiated at a dose of 400 Gy. After irradiation, the surviving cells were stained with Nile red. The mutant (Cr-4013) accumulating 20% more lipid than the wild type was selected. Thin-layer chromatography revealed the triglyceride and free fatty acid contents to be markedly increased in Cr-4013. The major fatty acids identified were palmitic acid, oleic acid, linoleic acid, and linolenic acid. Random amplified polymeric DNA analysis showed partial genetic modifications in Cr-4013. To ascertain the changes of protein expression in the mutant strain, two-dimensional electrophoresis was conducted. These results showed that gamma radiation could be used for the development of efficient microalgal strains for lipid production.
authors: Department of Biotechnology and Bioengineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University, Gwangju 61186, Republic of Korea.
authors: Jaewon
authors: J
authors: Baek
authors: Department of Biotechnology and Bioengineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University, Gwangju 61186, Republic of Korea.
authors: Jong-Il
authors: JI
authors: Choi
authors: Korea Polar Research Institute, Incheon 21990, Republic of Korea.
authors: Hyun
authors: H
authors: Park
authors: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea.
authors: Sangyong
authors: S
authors: Lim
authors: Department of Environmental Engineering and Energy, Myongji University, Yongin 17058, Republic of Korea.
authors: Si Jae
authors: SJ
authors: Park
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*; D005720:Gamma Rays; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry; D009154:Mutation; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D008055:Lipids
keywords: Chlamydomonas reinhardtii; lipid content; mutant; proteome