title: Oxidative stress in carotid arteries of patients submitted to carotid endarterectomy. The role of aging process.
abstract: OBJECTIVE
  To evaluated the role of oxidative stress on aging process in patients submitted to carotid endarterectomy.
  
  
  METHODS
  Twenty patients were divided into two groups: older group (≥ 70 years old); and the younger group (< 70 years old). We evaluated the reactive oxygen species (ROS) concentration, nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase, superoxide dismutase (SOD) and catalase (CAT) activities as so as nitrite levels in fragments of carotid arteries harvested during carotid endarterectomy for treatment of high grade carotid stenosis.
  
  
  RESULTS
  We observed a higher levels of ROS and NADPH oxidase activity in the older group (p<0.05). Furthermore, the nitrite concentration was lower in the older group (14.55 ± 5.61 x 10-3 versus 26.42 ± 8.14 x 10-3 µM; p=0.0123). However, the activities of antioxidant enzymes (CAT and SOD) were similar in both the groups.
  
  
  CONCLUSIONS
  : Arterial aging is associated with increased concentrations of oxygen species and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity as so as nitrite reduction in human carotid artery specimens. Maybe therapies that block NADPH oxidase activity and enhance nitrite stores would be a good strategy to reduce the effect of oxidative stress in arterial aging.
authors: Master in Medicine, Vascular Surgeon, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil. Technical procedures, acquisition and interpretation of data, manuscript writing, critical revision.
authors: Márcio Luís
authors: ML
authors: Lucas
authors: PhD, Department of Cardiovascular Physiology, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre-RS, Brazil. Technical procedures, interpretation of data, critical revision.
authors: Cristina Campos
authors: CC
authors: Carraro
authors: PhD, Associate Professor, Department of Physiology, UFRGS, Porto Alegre-RS, Brazil. Technical procedures, interpretation of data.
authors: Adriane
authors: A
authors: Belló-Klein
authors: PhD, Associate Professor, Department of Surgery, UFSCPA, Porto Alegre-RS, Brazil. Technical procedures, acquisition of data.
authors: Antônio Nocchi
authors: AN
authors: Kalil
authors: PhD, Chairman and Head, Department of Vascular Surgery, UFSCPA, Porto Alegre-RS, Brazil. Interpretation of data, critical revision.
authors: Newton
authors: N
authors: Aerts
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D002339:Carotid Arteries / Q000201:enzymology / Q000502:physiology*; D002374:Catalase / Q000378:metabolism; D003324:Coronary Artery Disease / Q000601:surgery; D016894:Endarterectomy, Carotid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009249:NADP / Q000032:analysis; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000032:analysis; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D009249:NADP; D002374:Catalase; D013482:Superoxide Dismutase