title: Graphene Oxide-Silver Nanocomposite: Novel Agricultural Antifungal Agent against Fusarium graminearum for Crop Disease Prevention.
abstract: Nanoparticle-based antibacterial agents have emerged as an interdisciplinary field involving medicine, material science, biology, and chemistry because of their size-dependent qualities, high surface-to-volume ratio, and unique physiochemical properties. Some of them have shown great promise for their application in plant protection and nutrition. Here, GO-AgNPs nanocomposite was fabricated through interfacial electrostatic self-assembly and its antifungal activity against phytopathogen Fusarium graminearum was investigated in vitro and in vivo for the first time. The results demonstrated that the GO-AgNPs nanocomposite showed almost a 3- and 7-fold increase of inhibition efficiency over pure AgNPs and GO suspension, respectively. The spore germination inhibition was stimulated by a relatively low concentration of 4.68 μg/mL (minimum inhibition concentration (MIC)). The spores and hyphae were damaged, which might be caused by an antibacterial mechanism from the remarkable synergistic effect of GO-AgNPs, inducing physical injury and chemical reactive oxygen species generation. More importantly, the chemical reduction of GO mediated by fungal spores was possibly contributed to the high antimicrobial activity of GO-AgNPs. Furthermore, the GO-AgNPs nanocomposite showed a significant effect in controlling the leaf spot disease infected by F. graminearum in the detached leaf experiment. All the results from this research suggest that the GO-AgNPs nanocomposite developed in this work has the potential as a promising material for the development of novel antimicrobial agents against pathogenic fungi or bacteria.
authors: State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, College of Science, Huazhong Agricultural University , Wuhan, 430070, P.R. China.|Laboratory of Natural Product Pesticide, College of Plant Protection, Southwest University , Chongqing, 400715, P.R. China.
authors: Juanni
authors: J
authors: Chen
authors: State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, College of Science, Huazhong Agricultural University , Wuhan, 430070, P.R. China.
authors: Long
authors: L
authors: Sun
authors: State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, College of Science, Huazhong Agricultural University , Wuhan, 430070, P.R. China.
authors: Yuan
authors: Y
authors: Cheng
authors: State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, College of Science, Huazhong Agricultural University , Wuhan, 430070, P.R. China.
authors: Zhicheng
authors: Z
authors: Lu
authors: State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, College of Science, Huazhong Agricultural University , Wuhan, 430070, P.R. China.
authors: Kang
authors: K
authors: Shao
authors: State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, College of Science, Huazhong Agricultural University , Wuhan, 430070, P.R. China.
authors: Tingting
authors: T
authors: Li
authors: State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, College of Science, Huazhong Agricultural University , Wuhan, 430070, P.R. China.
authors: Chao
authors: C
authors: Hu
authors: State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, College of Science, Huazhong Agricultural University , Wuhan, 430070, P.R. China.
authors: Heyou
authors: H
authors: Han
mesh_terms: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D005670:Fusarium; D006108:Graphite; D053768:Metal Nanoparticles; D053761:Nanocomposites; D010087:Oxides; D012834:Silver
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010087:Oxides; D012834:Silver; D006108:Graphite
keywords: Fusarium graminearum; GO-AgNPs nanocomposite; antifungal activity; detached leaf experiment; synergistic effect