title: Efficient transformation of Staphylococcus aureus using multi-pulse electroporation.
abstract: A new multi-pulse electroporation system was evaluated to transform Staphylococcus aureus. Compared to the conventional electroporation system, it yielded high transformation efficiency to obtain more than 3.9×105S. aureus RN4220 transformed cells/1μg plasmid DNA using a single electroporation by manipulating the poring pulse and transfer pulse.
authors: Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan.
authors: Junzo
authors: J
authors: Hisatsune
authors: Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan.
authors: Yusuke
authors: Y
authors: Sato'o
authors: Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan.
authors: Liansheng
authors: L
authors: Yu
authors: Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan.
authors: Shoko
authors: S
authors: Kutsuno
authors: Nepa Gene Co., Ltd., Chiba, Japan.
authors: Yasuhiko
authors: Y
authors: Hayakawa
authors: Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima, Japan; Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan. Electronic address: sugai@hiroshima-u.ac.jp.
authors: Motoyuki
authors: M
authors: Sugai
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004553:Electric Conductivity; D004560:Electricity; D018274:Electroporation / Q000295:instrumentation* / Q000379:methods*; D005821:Genetic Techniques / Q000295:instrumentation; D010957:Plasmids; D013211:Staphylococcus aureus / Q000235:genetics*; D013696:Temperature; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial
keywords: Competency; ELEPO21; Multi-pulse electroporation; Plasmid DNA; Poring pulse; Staphylococcus aureus; Transfer pulse; Transformation