title: Relationship Between Cytogenetic Complexity and Peritumoral Edema in High-Grade Astrocytoma.
abstract: BACKGROUND
  The purpose of the study is to reveal the association of cytogenetic compltyexi and peritumoral edema volume (PTEV) and its prognostic significance in high-grade astrocytoma patients by culturing patient tumor cells.
  
  
  METHODS
  Twenty-seven high-grade astrocytoma patients were divided into three groups according to karyotype complexity: normal, non-complex karyotype (NCK), and complex karyotype (CK). Endothelial growth factor receptor (EGFR) amplification was detected by FISH, and its association with chromosome 7 abnormalities was analyzed. Mean PTEV of each group was compared by ANOVA to evaluate the relationship between PTEV and cytogenetic complexity.
  
  
  RESULTS
  The PTEV of patients in normal (n=6), NCK (n=8), and CK (n=13) groups were 24.52±17.73, 34.26±35.04, and 86.31±48.7 cm³, respectively (P=0.005). Ten out of 11 patients with EGFR amplification showed abnormalities in chromosome 7. The mean PTEV of EGFR-amplified and non-amplified groups were 80.4±53.7 and 41.3±37.9 cm³, respectively (P=0.035). The average survival of patients with PTEV less than 90 cm³ was 30.52±26.11 months, while in patients with PTEVs over or equal to 90 cm³, it was 10.83±5.53 months (P=0.007).
  
  
  CONCLUSIONS
  The results show an association of complex karyotype with the PTEV of high-grade astrocytoma. EGFR amplification plays a significant role in the formation of peritumoral edema, causing PTEV to increase, which is related with survival. This implies that cytogenetic karyotype can be applied as a prognostic factor.
authors: Department of Neurosurgery, Medical Science Research Center, College of Medicine, Dong-A University, Busan, Korea.
authors: Kyung Ho
authors: KH
authors: Jeong
authors: Department of Neurosurgery, Medical Science Research Center, College of Medicine, Dong-A University, Busan, Korea.|Brain Tumor Institute, Medical Science Research Center, College of Medicine, Dong-A University, Busan, Korea.
authors: Young Jin
authors: YJ
authors: Song
authors: Department of Laboratory Medicine, College of Medicine, Dong-A University, Busan, Korea.
authors: Jin Yeong
authors: JY
authors: Han
authors: Department of Neurosurgery, Medical Science Research Center, College of Medicine, Dong-A University, Busan, Korea.|Brain Tumor Institute, Medical Science Research Center, College of Medicine, Dong-A University, Busan, Korea. kukim@donga.ac.kr.
authors: Ki Uk
authors: KU
authors: Kim
mesh_terms: D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000000981:diagnostic imaging / Q000401:mortality / Q000473:pathology*; D001932:Brain Neoplasms / Q000000981:diagnostic imaging / Q000401:mortality / Q000473:pathology*; D002869:Chromosome Aberrations; D002897:Chromosomes, Human, Pair 7; D004487:Edema / Q000000981:diagnostic imaging / Q000473:pathology; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D053208:Kaplan-Meier Estimate; D059785:Karyotype; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D011379:Prognosis; D040262:Receptors, Vascular Endothelial Growth Factor / Q000378:metabolism; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: D040262:Receptors, Vascular Endothelial Growth Factor
keywords: Chromosome; EGFR amplification; High-grade astrocytoma; Peritumoral edema