title: Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy.
abstract: OBJECTIVE
  The aim of this study was to explore the tape strip sampling technique in the assessment of stratum corneum levels of inflammatory mediators in a clinical trial setting.
  
  
  METHODS
  Thirty-eight inflammatory mediators were analyzed by a multiplex-assay in the stratum corneum, collected by adhesive tapes before and after 6 weeks of therapy, in mild and moderate atopic dermatitis (AD) patients (n = 90). Treatment was a ceramide- and magnesium-containing emollient.
  
  
  RESULTS
  Twenty-four mediators could quantitatively be determined. The Th2 mediators interleukin (IL)-4, IL-13, CCL2 (monocyte chemotactic protein-1), CCL22 (macrophage-derived chemokine), and CCL17 [thymus and activation-regulated chemokine (TARC)] were significantly decreased after therapy as well as IL-1β, IL-2, IL-8 (CXCL8), IL-10, acute-phase protein serum amyloid A, C-reactive protein, and vascular adhesion molecule-1. The decrease of CCL17 and IL-8 was correlated with the decrease in disease severity in a subgroup of moderate AD individuals.
  
  
  CONCLUSIONS
  Stratum corneum tape stripping offers a minimally invasive approach for studying local levels of immunomodulatory molecules in the skin. CCL17 (TARC) and IL-8 were found to be the most promising biomarkers of AD and might be useful for investigating the course of skin diseases and the effect of local therapy.
authors: Coronel Institute of Occupational Health, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors: Sjors A
authors: SA
authors: Koppes
authors: Richard
authors: R
authors: Brans
authors: Suzana
authors: S
authors: Ljubojevic Hadzavdic
authors: Monique H W
authors: MH
authors: Frings-Dresen
authors: Thomas
authors: T
authors: Rustemeyer
authors: Sanja
authors: S
authors: Kezic
mesh_terms: D000287:Administration, Topical; D000328:Adult; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000175:diagnosis* / Q000378:metabolism* / Q000628:therapy; D004643:Emollients / Q000008:administration & dosage; D004817:Epidermis / Q000378:metabolism*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D053832:Surgical Tape; D055815:Young Adult
chemical_list: D016207:Cytokines; D004643:Emollients; D018836:Inflammation Mediators