title: Novel Fluorescence Arginine Analogue as a Sensor for Direct Identification and Imaging of Nitric Oxide Synthase-like Enzymes in Plants.
abstract: Nitric oxide synthase like enzyme (NOS-like enzyme), which produces nitric oxide, participates in many biological processes. However it remains unidentified and highly controversial that plants do possess a NOS-like enzyme. In this paper, a novel arginine analogue NP1 was designed and developed for the direct identification and real time tracking of NOS-like enzymes in plant by fluorescence sensing. It could bind NOS-like enzyme efficiently and enter the cell successfully. In vivo fluorescence response results directly proved that NOS-like enzymes did exist in tobacco leaf and would be stimulated by pathogen infection, which also provided a useful chemical tool for the study of the function of NOS-like enzyme in plants.
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors: Kang
authors: K
authors: Chang
authors: National Key Laboratory of Plant Molecular Genetics; Shanghai Institute of Plan Physiology and Ecology; Shanghai Institutes for Biological Sciences; Chinese Academy of Sciences; Shanghai, China.
authors: Tongtong
authors: T
authors: Guo
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors: Pengfei
authors: P
authors: Li
authors: National Key Laboratory of Plant Molecular Genetics; Shanghai Institute of Plan Physiology and Ecology; Shanghai Institutes for Biological Sciences; Chinese Academy of Sciences; Shanghai, China.
authors: Yin
authors: Y
authors: Liu
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors: Yufang
authors: Y
authors: Xu
authors: National Key Laboratory of Plant Molecular Genetics; Shanghai Institute of Plan Physiology and Ecology; Shanghai Institutes for Biological Sciences; Chinese Academy of Sciences; Shanghai, China.
authors: Yuda
authors: Y
authors: Fang
authors: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors: Xuhong
authors: X
authors: Qian
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D019441:Plant Epidermis / Q000166:cytology; D018515:Plant Leaves / Q000201:enzymology; D014026:Nicotiana / Q000201:enzymology*
chemical_list: D005456:Fluorescent Dyes; D001120:Arginine; D019001:Nitric Oxide Synthase