title: Construction of a high-density genetic map and QTL mapping for pearl quality-related traits in Hyriopsis cumingii.
abstract: A high-density genetic map is essential for quantitative trait locus (QTL) fine mapping. In this study, 4,508 effective single nucleotide polymorphism markers (detected using specific-locus amplified fragment sequencing) and 475 microsatellites were mapped to 19 linkage groups (LGs) using a family with 157 individuals. The map spanned 2,713 cM, with an average of 259 markers and 79 loci per LG and an average inter-marker distance of 1.81 cM. To identify QTLs for pearl quality traits, 26 putatively significant QTLs were detected for 10 traits, including, three for shell width, seven for body weight, two for shell weight, two for margin mantle weight, five for inner mantle weight, and seven for shell nacre colour. Among them, five QTLs associated with shell nacre colour were mapped to LG17 and explained 19.7% to 22.8% of the trait variation; this suggests that some important genes or loci determine shell nacre colour in LG17. The linkage map and mapped QTLs for shell nacre colour would be useful for improving the quality of Hyriopsis cumingii via marker-assisted selection.
authors: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306, China.|Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai 201306, China.
authors: Zhi-Yi
authors: ZY
authors: Bai
authors: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306, China.
authors: Xue-Kai
authors: XK
authors: Han
authors: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306, China.
authors: Xiao-Jun
authors: XJ
authors: Liu
authors: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306, China.
authors: Qing-Qing
authors: QQ
authors: Li
authors: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306, China.|Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai 201306, China.|Aquaculture Division, E-Institute of Shanghai Universities, Shanghai Ocean University, Shanghai 201306, China.
authors: Jia-Le
authors: JL
authors: Li
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D020090:Chromosome Segregation / Q000235:genetics; D003433:Crosses, Genetic; D057225:Data Mining; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers