title: Asymmetrical flow field-flow fractionation coupled with multiple detections: A complementary approach in the characterization of egg yolk plasma.
abstract: The capability of asymmetrical flow field-flow fractionation (AF4) coupled with UV/VIS, multiangle light scattering (MALS) and quasi-elastic light scattering (QELS) (AF4-UV-MALS-QELS) for separation and characterization of egg yolk plasma was evaluated. The accuracy of hydrodynamic radius (Rh) obtained from QELS and AF4 theory (using both simplified and full expression of AF4 retention equations) was discussed. The conformation of low density lipoprotein (LDL) and its aggregates in egg yolk plasma was discussed based on the ratio of radius of gyration (Rg) to Rh together with the results from bio-transmission electron microscopy (Bio-TEM). The results indicate that the full retention equation is more relevant than simplified version for the Rh determination at high cross flow rate. The Rh from online QELS is reliable only at a specific range of sample concentration. The effect of programmed cross flow rate (linear and exponential decay) on the analysis of egg yolk plasma was also investigated. It was found that the use of an exponentially decaying cross flow rate not only reduces the AF4 analysis time of the egg yolk plasma, but also provides better resolution than the use of either a constant or linearly decaying cross flow rate. A combination of an exponentially decaying cross flow AF4-UV-MALS-QELS and the utilization of full retention equation was proved to be a useful method for the separation and characterization of egg yolk plasma.
authors: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, School of Medicine, Hebei University, Baoding 071002, China. Electronic address: douhaiyang-1984@163.com.
authors: Haiyang
authors: H
authors: Dou
authors: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, School of Medicine, Hebei University, Baoding 071002, China.
authors: Yueqiu
authors: Y
authors: Li
authors: Department of Chemistry, Hannam University, Daejeon 305811, Republic of Korea.
authors: Jaeyeong
authors: J
authors: Choi
authors: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, School of Medicine, Hebei University, Baoding 071002, China.
authors: Shuying
authors: S
authors: Huo
authors: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, School of Medicine, Hebei University, Baoding 071002, China.
authors: Liang
authors: L
authors: Ding
authors: Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, School of Medicine, Hebei University, Baoding 071002, China. Electronic address: shensg@hbu.edu.cn.
authors: Shigang
authors: S
authors: Shen
authors: Department of Chemistry, Hannam University, Daejeon 305811, Republic of Korea. Electronic address: slee@hnu.kr.
authors: Seungho
authors: S
authors: Lee
mesh_terms: D000818:Animals; D002645:Chickens; D004530:Egg Yolk / Q000737:chemistry*; D005260:Female; D031521:Fractionation, Field Flow; D057446:Hydrodynamics; D008027:Light; D008077:Lipoproteins, LDL / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet
chemical_list: D008077:Lipoproteins, LDL
keywords: Asymmetrical flow field-flow fractionation; Characterization; Egg yolk plasma; Exponentially decaying cross flow; Light scattering