title: Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging.
abstract: Hepatic fibrosis is a chronic disorder caused by viral infection and/or metabolic, genetic and cholestatic disorders. A noninvasive procedure that enables the detection of liver fibrosis based on redox status would be useful for disease identification and monitoring, and the development of treatments. However, an appropriate technique has not been reported. This study describes a novel method for assessing the redox status of the liver using in vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) with the nitroxyl radical carbamoyl-PROXYL as a molecular imaging probe, which was tested in dimethylnitrosamine-treated mice as a model of liver fibrosis. Based on the pharmacokinetics of carbamoyl-PROXYL in control livers, reduction rate mapping was performed in fibrotic livers. Reduction rate maps demonstrated a clear difference between the redox status of control and fibrotic livers according to the expression of antioxidants. These findings indicate that in vivo DNP-MRI with a nitroxyl radical probe enables noninvasive detection of changes in liver redox status.
authors: Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.|Department of Advanced Medical Initiatives, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.
authors: Takahito
authors: T
authors: Kawano
authors: Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.|Department of Advanced Medical Initiatives, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.|Center for Advanced Medical Innovation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors: Masaharu
authors: M
authors: Murata
authors: Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.
authors: Fuminori
authors: F
authors: Hyodo
authors: Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.
authors: Hinako
authors: H
authors: Eto
authors: Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.
authors: Nuttavut
authors: N
authors: Kosem
authors: Department of Advanced Medical Initiatives, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.|Center for Advanced Medical Innovation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors: Ryosuke
authors: R
authors: Nakata
authors: Faculty of Pharmaceutical Science, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada.
authors: Nobuhito
authors: N
authors: Hamano
authors: Department of Advanced Medical Initiatives, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.|Center for Advanced Medical Innovation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors: Jing Shu
authors: JS
authors: Piao
authors: Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.|Department of Advanced Medical Initiatives, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.
authors: Sayoko
authors: S
authors: Narahara
authors: Department of Advanced Medical Initiatives, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.
authors: Tomohiko
authors: T
authors: Akahoshi
authors: Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.|Department of Advanced Medical Initiatives, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku,, Fukuoka 812-8582, Japan.|Center for Advanced Medical Innovation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors: Makoto
authors: M
authors: Hashizume
mesh_terms: D000818:Animals; D003497:Cyclic N-Oxides / Q000097:blood; D004128:Dimethylnitrosamine; D007275:Injections, Intravenous; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000097:blood / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D008297:Male; D008807:Mice, Inbred BALB C; D009589:Nitrogen Oxides / Q000008:administration & dosage; D010084:Oxidation-Reduction
chemical_list: D003497:Cyclic N-Oxides; D009589:Nitrogen Oxides; C039900:nitroxyl; D004128:Dimethylnitrosamine