title: Hmgn5 functions downstream of Hoxa10 to regulate uterine decidualization in mice.
abstract: Although Hmgn5 is involved in the regulation of cellular proliferation and differentiation, its physiological function during decidualization is still unknown. Here we showed that Hmgn5 was highly expressed in the decidual cells. Silencing of Hmgn5 expression by specific siRNA reduced the proliferation of uterine stromal cells and expression of Ccnd3 and Cdk4 in the absence or presence of estrogen and progesterone, whereas overexpression of Hmgn5 exhibited the opposite effects. Simultaneously, Hmgn5 might induce the expression of Prl8a2 and Prl3c1 which were 2 well-known differentiation markers for decidualization. In the uterine stromal cells, cAMP analog 8-Br-cAMP and progesterone could up-regulate the expression of Hmgn5, but the up-regulation was impeded by H89 and RU486, respectively. Attenuation of Hmgn5 expression could block the differentiation of uterine stromal cells in response to cAMP and progesterone. Further studies found that regulation of cAMP and progesterone on Hmgn5 expression was mediated by Hoxa10. During in vitro decidualization, knockdown of Hmgn5 could abrogate Hoxa10-induced upregulation of Prl8a2 and Prl3c1, while overexpression of Hmgn5 reversed the inhibitory effects of Hoxa10 siRNA on the expression of Prl8a2 and Prl3c1. In the stromal cells undergoing decidualization, Hmgn5 might act downstream of Hoxa10 to regulate the expression of Cox-2, Vegf and Mmp2. Collectively, Hmgn5 may play an important role during mouse decidualization.
authors: a College of Veterinary Medicine , Jilin University , Changchun , P. R. China.
authors: Dang-Dang
authors: DD
authors: Li
authors: a College of Veterinary Medicine , Jilin University , Changchun , P. R. China.
authors: Shu-Yi
authors: SY
authors: Zhao
authors: a College of Veterinary Medicine , Jilin University , Changchun , P. R. China.
authors: Zhan-Qing
authors: ZQ
authors: Yang
authors: b Institute of Agro-food Technology , Jilin Academy of Agricultural Sciences , Changchun , P. R. China.
authors: Cui-Cui
authors: CC
authors: Duan
authors: a College of Veterinary Medicine , Jilin University , Changchun , P. R. China.
authors: Chuan-Hui
authors: CH
authors: Guo
authors: a College of Veterinary Medicine , Jilin University , Changchun , P. R. China.
authors: Hong-Liang
authors: HL
authors: Zhang
authors: a College of Veterinary Medicine , Jilin University , Changchun , P. R. China.
authors: Shuang
authors: S
authors: Geng
authors: a College of Veterinary Medicine , Jilin University , Changchun , P. R. China.
authors: Zhan-Peng
authors: ZP
authors: Yue
authors: a College of Veterinary Medicine , Jilin University , Changchun , P. R. China.
authors: Bin
authors: B
authors: Guo
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D000242:Cyclic AMP / Q000494:pharmacology; D051546:Cyclooxygenase 2 / Q000378:metabolism; D003656:Decidua / Q000166:cytology / Q000378:metabolism*; D005260:Female; D024001:HMGN Proteins / Q000235:genetics / Q000378:metabolism*; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000378:metabolism*; D017403:In Situ Hybridization; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051379:Mice; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D024001:HMGN Proteins; C498963:Hmgn5 protein, mouse; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C092119:Hoxa10 protein, mouse; D011374:Progesterone; D000242:Cyclic AMP; D051546:Cyclooxygenase 2; D020778:Matrix Metalloproteinase 2
keywords: Hmgn5; decidualization; mouse; stromal cell; uterus