title: Quantitative analysis of biomarkers of liver and kidney injury in serum and urine using ultra-fast liquid chromatography with tandem mass spectrometry coupled with a hydrophilic interaction chromatography column: Application to monitor injury induced by Euphorbiae pekinensis Radix.
abstract: Fast and sensitive monitoring of drug-induced liver and kidney injury in early stage is beneficial. An ultrafast liquid chromatography with tandem mass spectrometry assay was developed and validated to simultaneously determine ten endogenous biomarkers in serum and urine, including hippuric acid, phenylacetylglycine, 5-oxoproline, cholic acid, taurine, indoleacetic acid, 3-indoxyl sulfate, guanidinosuccinic acid, guanidinoacetic acid and uric acid. A CAPCELL CORE PC column (2.1 × 150 mm, 2.7 μm) was adopted for analytes separation. Gradient elution was performed with acetonitrile and water containing 5 mM ammonium acetate. Simple protein precipitation was applied in sample preparation. Good linearities were achieved with all the regression coefficients above 0.9911. Accuracy was 2.9-14.2% in serum and 4.1-14.6% in urine. The mean recovery was above 70% with acceptable matrix effects. The method was applied to monitor injury induced by Euphorbiae pekinensis Radix with a subacute rats model. All the biomarkers showed obvious concentration changes during the injury period. Furthermore, several biomarkers showed significant changes in earlier stage when compared with the current clinical serum bio-parameters. The method might be helpful for early diagnosis of drug induced liver and kidney injury in clinical after tested on more drugs.
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
authors: Yuanyuan
authors: Y
authors: Zhang
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
authors: Liqiang
authors: L
authors: Gu
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
authors: Yu
authors: Y
authors: Jiang
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
authors: Kaishun
authors: K
authors: Bi
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China. cxh_syphu@hotmail.com.
authors: Xiaohui
authors: X
authors: Chen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood* / Q000652:urine*; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D019665:Euphorbiaceae / Q000633:toxicity*; D057927:Hydrophobic and Hydrophilic Interactions; D051381:Rats; D051437:Renal Insufficiency / Q000097:blood* / Q000139:chemically induced / Q000652:urine*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers
keywords: Biomarkers; Euphorbiae pekinensis Radix; Hydrophilic interaction columns; Kidney injury; Liver injury