title: A Surveillance Function of the HSPB8-BAG3-HSP70 Chaperone Complex Ensures Stress Granule Integrity and Dynamism.
abstract: Stress granules (SGs) are ribonucleoprotein complexes induced by stress. They sequester mRNAs and disassemble when the stress subsides, allowing translation restoration. In amyotrophic lateral sclerosis (ALS), aberrant SGs cannot disassemble and therefore accumulate and are degraded by autophagy. However, the molecular events causing aberrant SG formation and the molecular players regulating this transition are largely unknown. We report that defective ribosomal products (DRiPs) accumulate in SGs and promote a transition into an aberrant state that renders SGs resistant to RNase. We show that only a minor fraction of aberrant SGs is targeted by autophagy, whereas the majority disassembles in a process that requires assistance by the HSPB8-BAG3-HSP70 chaperone complex. We further demonstrate that HSPB8-BAG3-HSP70 ensures the functionality of SGs and restores proteostasis by targeting DRiPs for degradation. We propose a system of chaperone-mediated SG surveillance, or granulostasis, which regulates SG composition and dynamics and thus may play an important role in ALS.
authors: Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena 41125, Italy.
authors: Massimo
authors: M
authors: Ganassi
authors: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany.
authors: Daniel
authors: D
authors: Mateju
authors: Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena 41125, Italy.
authors: Ilaria
authors: I
authors: Bigi
authors: Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena 41125, Italy.
authors: Laura
authors: L
authors: Mediani
authors: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany.
authors: Ina
authors: I
authors: Poser
authors: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany.
authors: Hyun O
authors: HO
authors: Lee
authors: Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena 41125, Italy.
authors: Samuel J
authors: SJ
authors: Seguin
authors: Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena 41125, Italy.
authors: Federica F
authors: FF
authors: Morelli
authors: Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena 41125, Italy.
authors: Jonathan
authors: J
authors: Vinet
authors: Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena 41125, Italy.
authors: Giuseppina
authors: G
authors: Leo
authors: Laboratory of Experimental Neurobiology, "C. Mondino" National Institute of Neurology Foundation, IRCCS, Via Mondino 2, 27100 Pavia, Italy.
authors: Orietta
authors: O
authors: Pansarasa
authors: Laboratory of Experimental Neurobiology, "C. Mondino" National Institute of Neurology Foundation, IRCCS, Via Mondino 2, 27100 Pavia, Italy.
authors: Cristina
authors: C
authors: Cereda
authors: Department of Pharmacological and Biomolecular Sciences, Centre of Excellence on Neurodegenerative Diseases University of Milan, Milan 20133, Italy.
authors: Angelo
authors: A
authors: Poletti
authors: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany. Electronic address: alberti@mpi-cbg.de.
authors: Simon
authors: S
authors: Alberti
authors: Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena 41125, Italy. Electronic address: serena.carra@unimore.it.
authors: Serena
authors: S
authors: Carra
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D018053:Arsenites / Q000494:pharmacology; D001343:Autophagy / Q000235:genetics*; D003594:Cytoplasmic Granules / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis; D006801:Humans; D007976:Leupeptins / Q000494:pharmacology; D018832:Molecular Chaperones; D018384:Oxidative Stress; D061988:Proteasome Inhibitors / Q000494:pharmacology; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D059748:Proteolysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D018053:Arsenites; C414431:BAG3 protein, human; D018840:HSP70 Heat-Shock Proteins; C413214:HSPB8 protein, human; D006360:Heat-Shock Proteins; D007976:Leupeptins; D018832:Molecular Chaperones; D061988:Proteasome Inhibitors; D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases; D012260:Ribonucleases; C015001:arsenite; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde