title: Investigation of analyte losses using microwave-assisted sample digestion and closed vessels with venting.
abstract: Microwave-assisted sample digestion using closed vessels is becoming the standard for trace analysis because contamination and losses can be better controlled. Gases are generated during digestion and there is an increment of the internal vessel pressure. Consequently, vessels venting may occur depending on the design of the vessel and the maximum pressure it can stand for. In the present work it was observed that it is possible to allow venting during the digestion without losing volatile analytes, such as As, when properly controlling heating and chemical conditions. Recoveries for As in certified reference materials of animal tissues ranged from 94 to 112% despite mass losses as high as 62%mm(-1) observed in the digests. However, for Hg(II) in medium containing chlorides recoveries were poor. The efficiency of digestion was measured by determination of organic carbon contents in digests and they ranged from 0.10 to 0.19% for plant and animal tissues. The temperature gradient along the vessel height is important for avoiding losses of volatile elements.
authors: Group for Applied Instrumental Analysis, Department of Chemistry, Federal University of São Carlos, P.O. Box 676, São Carlos, SP, 13560-970 Brazil. Electronic address: clariceamaral@yahoo.com.br.
authors: Clarice D B
authors: CDB
authors: Amaral
authors: Group for Applied Instrumental Analysis, Department of Chemistry, Federal University of São Carlos, P.O. Box 676, São Carlos, SP, 13560-970 Brazil.
authors: Lucimar L
authors: LL
authors: Fialho
authors: Group for Applied Instrumental Analysis, Department of Chemistry, Federal University of São Carlos, P.O. Box 676, São Carlos, SP, 13560-970 Brazil.
authors: Felipe P R
authors: FPR
authors: Camargo
authors: Milestone, Sorisole, BG, Italy.
authors: Camillo
authors: C
authors: Pirola
authors: Group for Applied Instrumental Analysis, Department of Chemistry, Federal University of São Carlos, P.O. Box 676, São Carlos, SP, 13560-970 Brazil.
authors: Joaquim A
authors: JA
authors: Nóbrega
keywords: Analyte losses; Sample preparation; Temperature gradient; Venting; Volatile elements