title: Grid-like surface structures in thermoplastic polyurethane induce anti-inflammatory and anti-fibrotic processes in bone marrow-derived mesenchymal stem cells.
abstract: The use of autologous cells for the coating of implant surfaces presents a promising tool to attenuate foreign body reaction and inflammation. However, insertion forces that occur especially during implantation of electrodes into the narrow cochlea may strip off cells from the surface. Thus, implant surfaces should be ideally structured in a way that protects the cell coating from mechanical removal during implantation. The structuring of implant surfaces may also direct cells towards desired functions to further enhance their performance and clinical suitability. In this study, grid-like square cavities were generated on thermoplastic polyurethane (TPU) surfaces using a combination of femtosecond laser ablation and replication methods. Afterwards, they were tested as potential scaffolds for human bone marrow-derived mesenchymal stem cells (MSCs) in order to use it on neural prostheses. Structured and non-structured TPU allowed proper adhesion and survival of MSCs. Surface structuring resulted in regulation of over 500 genes. Many of the upregulated genes are known to be involved in anti-inflammatory, anti-fibrotic and wound healing processes whereas genes relevant for mesenchymal differentiation programs were downregulated. The enhanced secretion of two representative factors (prostaglandin E2 and interleukin-1 receptor antagonist, respectively) was confirmed by ELISA and the downregulation of other genes involved in adipogenic and osteogenic differentiation were confirmed by gene expression analysis for a cultivation period of up to 21 days. In addition, mRNA of the surface antigens CD24 and ENDOGLIN (CD105) as representative factors for stemness did not show notable variation between cultivation on structured versus non-structured TPU or between 7 versus 21days of cultivation. Thus, surface topography of TPU seems to be a powerful tool to protect cells from mechanical forces during insertion and to influence cell behaviour.
authors: Department of Orthopaedic Surgery, Hannover Medical School, Anna von Borries-Str. 1-7, 30625 Hannover, Germany.
authors: Yvonne
authors: Y
authors: Roger
authors: Department of Otorhinolaryngology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany; Trauma Department, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors: Luisa Marilena
authors: LM
authors: Schäck
authors: LaserZentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany.
authors: Anastasia
authors: A
authors: Koroleva
authors: Trauma Department, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors: Sandra
authors: S
authors: Noack
authors: LaserZentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany.
authors: Kestutis
authors: K
authors: Kurselis
authors: Trauma Department, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors: Christian
authors: C
authors: Krettek
authors: LaserZentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany.
authors: Boris
authors: B
authors: Chichkov
authors: Department of Otorhinolaryngology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany; Cluster of Excellence "Hearing4all" of the German Research Foundation, Germany.
authors: Thomas
authors: T
authors: Lenarz
authors: Department of Otorhinolaryngology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany; Cluster of Excellence "Hearing4all" of the German Research Foundation, Germany. Electronic address: warnecke.athanasia@mh-hannover.de.
authors: Athanasia
authors: A
authors: Warnecke
authors: Department of Orthopaedic Surgery, Hannover Medical School, Anna von Borries-Str. 1-7, 30625 Hannover, Germany; Trauma Department, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
authors: Andrea
authors: A
authors: Hoffmann
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D005260:Female; D005355:Fibrosis / Q000517:prevention & control*; D006801:Humans; D007249:Inflammation / Q000517:prevention & control*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D010969:Plastics; D011140:Polyurethanes / Q000737:chemistry*; D055815:Young Adult
chemical_list: D010969:Plastics; D011140:Polyurethanes
keywords: Inflammation; Mesenchymal stem cell; Neural prosthesis; Polyurethane; Surface topography