title: Quantitative determination of a synthetic amide derivative of gallic acid, SG-HQ2, using liquid chromatography tandem mass spectrometry, and its pharmacokinetics in rats.
abstract: An amide derivative of gallic acid (GA), 3,4,5-trihydroxy-N-(8-hydroxyquinolin-2-yl)benzamide) (SG-HQ2) was recently synthesized, and its inhibitory actions were previously shown on histamine release and pro-inflammatory cytokine expression. In this study, a simultaneous quantification method was developed for the determination of SG-HQ2 and its possible metabolite, GA, in rat plasma using liquid chromatography with a tandem mass spectrometry (LC-MS/MS). After simple protein precipitation with acetonitrile including diclofenac (internal standard, IS), the analytes were chromatographed on a reversed phased column with a mobile phase of acetonitrile and water (60:40, v/v, including 0.1% formic acid). The ion transitions of the precursor to the product ion were principally protonated ion [M+H]+at m/z 313.2→160.6 for SG-HQ2, and deprotonated ions [M-H]-at m/z 168.7→124.9 for GA and 296.0→251.6 for the IS. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This method was successfully applied to a pharmacokinetic study of SG-HQ2 after intravenous administration in rats.
authors: College of Pharmacy, Gacheon University, Incheon, 21936 South Korea.
authors: Seung-Yong
authors: SY
authors: Seo
authors: College of Pharmacy, Chung-Ang University, Seoul, 06974 South Korea. Electronic address: wkang@cau.ac.kr.
authors: Wonku
authors: W
authors: Kang
mesh_terms: D000577:Amides / Q000032:analysis / Q000493:pharmacokinetics; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000493:pharmacokinetics*; D006912:Hydroxyquinolines / Q000032:analysis* / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000577:Amides; D006912:Hydroxyquinolines; C000599965:SG-HQ2; D005707:Gallic Acid
keywords: Gallic acid; LC–MS/MS; Pharmacokinetics; Rat; SG-HQ2