title: An FT-IR Spectral Analysis of the Effects of γ-Radiation on Normal and Cancerous Cartilage.
abstract: In the present study we used non-distractive physicochemical methods to investigate the effect of γ-radiation on human articular cartilage. Comparison between the FT-IR (Fourier transform infrared) spectra before and after irradiation of the cartilage with different doses of radiation showed considerable changes in the spectra. It was found that for doses up to 2 Gy the collagen helices changed their structure from α-helix to random coil. By increasing the radiation dose it was found that the proteins' structure changed further to amyloid-like protein formation and to fragments of glycosaminoglycan chains, which were indicated in the IR spectra. Furthermore, comparison between the spectra of normal and irradiated cartilage, cancerous cartilage and cartilage from patients who received radiotherapy showed similarities in the spectra together with the formation of an aldehyde absorption band at 1740 cm(-1) suggesting that in all cases of cartilage examined,oxidative stress played major role in the damage progression of cartilage.
authors: School of Chemical Engineering Laboratory of Radiation Chemistry & Biospectroscopy, National Technical University of Athens, Athens, Greece.
authors: Maria
authors: M
authors: Kyriakidou
authors: First Department of Orthopaedics, National and Kapodistrian University of Athens, Athens, Greece.
authors: Andreas F
authors: AF
authors: Mavrogenis
authors: School of Chemical Engineering Laboratory of Radiation Chemistry & Biospectroscopy, National Technical University of Athens, Athens, Greece.
authors: Stylianos
authors: S
authors: Kyriazis
authors: 2 Radiation Oncology Department, General Metaxa Anticancer Hospital of Piraeus, Piraeus, Greece.
authors: Athina
authors: A
authors: Markouizou
authors: School of Chemical Engineering Laboratory of Radiation Chemistry & Biospectroscopy, National Technical University of Athens, Athens, Greece.
authors: Theophile
authors: T
authors: Theophanides
authors: School of Chemical Engineering Laboratory of Radiation Chemistry & Biospectroscopy, National Technical University of Athens, Athens, Greece ianastas@central.ntua.gr.
authors: Jane
authors: J
authors: Anastassopoulou
mesh_terms: D002358:Cartilage, Articular / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D003094:Collagen / Q000737:chemistry* / Q000528:radiation effects; D005720:Gamma Rays / Q000009:adverse effects*; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D018384:Oxidative Stress / Q000528:radiation effects*; D011487:Protein Conformation / Q000528:radiation effects; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D006025:Glycosaminoglycans; D003094:Collagen
keywords: Cartilage; SEM; cancer; free radicals; infrared; γ-rays