title: Fluorescence-Activated Nucleolus Sorting in Arabidopsis.
abstract: Nucleolar isolation allows exhaustive characterization of the nucleolar content. Centrifugation-based protocols are not adapted to isolation of nucleoli directly from a plant tissue because of copurification of cellular debris. We describe here a method that allows the purification of nucleoli using fluorescent-activated cell sorting from Arabidopsis thaliana leaves. This approach requires the expression of a specific nucleolar protein such as fibrillarin fused to green fluorescent protein in planta.
authors: CNRS, Laboratoire Génome et Développement des Plantes, UMR5096, 58 Avenue P. Alduy, 66860, Perpignan, France. frederic.pontvianne@univ-perp.fr.|University Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR5096, 66860, Perpignan, France. frederic.pontvianne@univ-perp.fr.
authors: Frédéric
authors: F
authors: Pontvianne
authors: UMR5535, Institut de Génétique Moléculaire de Montpellier, 1919 route de Mende, 34293, Montpellier cedex 5, France.|MRI UMS3426, BioCampus Montpellier, c/o IGF, 141 rue de la cardonille, 34094, Montpellier cedex 5, France.
authors: Myriam
authors: M
authors: Boyer-Clavel
authors: CNRS, Laboratoire Génome et Développement des Plantes, UMR5096, 58 Avenue P. Alduy, 66860, Perpignan, France.|University Perpignan Via Domitia, Laboratoire Génome et Développement des Plantes, UMR5096, 66860, Perpignan, France.
authors: Julio
authors: J
authors: Sáez-Vásquez
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D002466:Cell Nucleolus / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D011993:Recombinant Fusion Proteins; C047458:fibrillarin; D049452:Green Fluorescent Proteins
keywords: Arabidopsis thaliana; FACS; Fibrillarin; Nucleolus