title: Extra Virgin Olive Oil Reduced Polyunsaturated Fatty Acid and Cholesterol Oxidation in Rodent Liver: Is This Accounted for Hydroxytyrosol-Fatty Acid Conjugation?
abstract: The effects of extra virgin olive oil (EVOO) and carbon tetrachloride (CCl4) induced oxidative stress in rats were determined by the generation of isoprostanoids. These are known to be robust biomarkers to evaluate nonenzymatic and free radical related oxidation. Other oxidative stress biomarkers such as hydroxyeicosatetraenoic acid products (HETEs) and cholesterol oxidation products (COPs) were also determined. The rodents received a control diet, high-fat diet (20% w/w) composed of extra virgin olive oil (EVOO), corn oil (CO), or lard, and high-fat diets with CCl4insult throughout the experimental period. The EVOO diet was found to suppress the formation of isoprostanoids and COPs compared to that of the control. EVOO also had a high total phenolic content and antioxidant activity compared to those of CO and lard and may be contributed to by the hydroxytyrosol component conjugated to fatty acids (HT-FA). This is the first study to identify HT-FA in EVOO, and it was 4-fold higher than that of olive oil, whereas none was found in corn oil. Furthermore, the EVOO diet showed reduced liver lipid vesicles in CCl4treated rats compared to that of the control. However, liver toxicity measurements of AST (aspartate transaminase) and ALT (alanine transaminase) activities showed augmentation with CCl4treatment but were not alleviated by the diets given. Our findings suggest that EVOO is a daily functional food capable of enhancing the antioxidant system for liver protection; the effect is potentially attributed to the phenolic and lipophenolic (phenol conjugated by fatty acids) content.
authors: School of Biological Sciences, The University of Hong Kong , Pokfulam Road, Hong Kong SAR.
authors: Yiu Yiu
authors: YY
authors: Lee
authors: Institut des Biomolécules Max Mousseron, UMR 5247, CNRS, Université de Montpellier & ENSCM (School of Chemistry), Faculté de Pharmacie , 15 Av. Charles Flahault, 34093 Montpellier cedex 05, France.
authors: Céline
authors: C
authors: Crauste
authors: School of Biological Sciences, The University of Hong Kong , Pokfulam Road, Hong Kong SAR.
authors: Hualin
authors: H
authors: Wang
authors: School of Biological Sciences, The University of Hong Kong , Pokfulam Road, Hong Kong SAR.
authors: Ho Hang
authors: HH
authors: Leung
authors: Institut des Biomolécules Max Mousseron, UMR 5247, CNRS, Université de Montpellier & ENSCM (School of Chemistry), Faculté de Pharmacie , 15 Av. Charles Flahault, 34093 Montpellier cedex 05, France.
authors: Joseph
authors: J
authors: Vercauteren
authors: Institut des Biomolécules Max Mousseron, UMR 5247, CNRS, Université de Montpellier & ENSCM (School of Chemistry), Faculté de Pharmacie , 15 Av. Charles Flahault, 34093 Montpellier cedex 05, France.
authors: Jean-Marie
authors: JM
authors: Galano
authors: Institut des Biomolécules Max Mousseron, UMR 5247, CNRS, Université de Montpellier & ENSCM (School of Chemistry), Faculté de Pharmacie , 15 Av. Charles Flahault, 34093 Montpellier cedex 05, France.
authors: Camille
authors: C
authors: Oger
authors: Institut des Biomolécules Max Mousseron, UMR 5247, CNRS, Université de Montpellier & ENSCM (School of Chemistry), Faculté de Pharmacie , 15 Av. Charles Flahault, 34093 Montpellier cedex 05, France.
authors: Thierry
authors: T
authors: Durand
authors: School of Biological Sciences, The University of Hong Kong , Pokfulam Road, Hong Kong SAR.
authors: Jennifer Man-Fan
authors: JM
authors: Wan
authors: School of Biological Sciences, The University of Hong Kong , Pokfulam Road, Hong Kong SAR.
authors: Jetty Chung-Yung
authors: JC
authors: Lee
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D000069463:Olive Oil / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D005231:Fatty Acids, Unsaturated; D000069463:Olive Oil; C005975:3,4-dihydroxyphenylethanol; D002784:Cholesterol; D010626:Phenylethyl Alcohol