title: Intravital Microscopy for Imaging the Tumor Microenvironment in Live Mice.
abstract: The development of intravital microscopy has provided unprecedented capacity to study the tumor microenvironment in live mice. The dynamic behavior of cancer, stromal, vascular, and immune cells can be monitored in real time, in situ, in both primary tumors and metastatic lesions, allowing treatment responses to be observed at single cell resolution and therapies tracked in vivo. These features provide a unique opportunity to elucidate the cellular mechanisms underlying the biology and treatment of cancer. We describe here a method for imaging the microenvironment of subcutaneous tumors grown in mice using intravital microscopy.
authors: Alberta Children's Hospital Research Institute, Calgary, AB, Canada, T2N 4N1.|Arnie Charbonneau Cancer Institute, Calgary, AB, Canada, T2N 4N1.|Snyder Institute for Chronic Diseases, Calgary, AB, Canada, T2N 4N1.|Department of Microbiology, Immunology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, AB, Canada, T2N 4N1.
authors: Victor
authors: V
authors: Naumenko
authors: Snyder Institute for Chronic Diseases, Calgary, AB, Canada, T2N 4N1. cnjenne@ucalgary.ca.|Department of Microbiology, Immunology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, AB, Canada, T2N 4N1. cnjenne@ucalgary.ca.
authors: Craig
authors: C
authors: Jenne
authors: Alberta Children's Hospital Research Institute, Calgary, AB, Canada, T2N 4N1. djmahone@ucalgary.ca.|Arnie Charbonneau Cancer Institute, Calgary, AB, Canada, T2N 4N1. djmahone@ucalgary.ca.|Department of Microbiology, Immunology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, AB, Canada, T2N 4N1. djmahone@ucalgary.ca.
authors: Douglas J
authors: DJ
authors: Mahoney
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D000069416:Intravital Microscopy / Q000295:instrumentation / Q000379:methods; D008113:Liver Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology*; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D059016:Tumor Microenvironment
keywords: Confocal imaging; Intravital microscopy; Mouse models; Multiphoton imaging; Subcutaneous tumors; Tumor microenvironment