title: Autologous subcutaneous adipose tissue transplants improve adipose tissue metabolism and reduce insulin resistance and fatty liver in diet-induced obesity rats.
abstract: Long-term dietary and pharmacological treatments for obesity have been questioned, particularly in individuals with severe obesity, so a new approach may involve adipose tissue transplants, particularly autologous transplants. Thus, the aim of this study was to evaluate the metabolic effects of autologous subcutaneous adipose tissue (SAT) transplants into two specific intraabdominal cavity sites (omental and retroperitoneal) after 90 days. The study was performed using two different diet-induced obesity (DIO) rat models: one using a high-fat diet (HFD) and the other using a high-carbohydrate diet (HCHD). Autologous SAT transplant reduced hypertrophic adipocytes, improved insulin sensitivity, reduced hepatic lipid content, and fasting serum-free fatty acids (FFAs) concentrations in the two DIO models. In addition, the reductions in FFAs and glycerol were accompanied by a greater reduction in lipolysis, assessed via the phosphorylation status of HSL, in the transplanted adipose tissue localized in the omentum compared with that localized in the retroperitoneal compartment. Therefore, the improvement in hepatic lipid content after autologous SAT transplant may be partially attributed to a reduction in lipolysis in the transplanted adipose tissue in the omentum due to the direct drainage of FFAs into the liver. The HCHD resulted in elevated fasting and postprandial serum insulin levels, which were dramatically reduced by the autologous SAT transplant. In conclusion, the specific intraabdominal localization of the autologous SAT transplant improved the carbohydrate and lipid metabolism of adipose tissue in obese rats and selectively corrected the metabolic parameters that are dependent on the type of diet used to generate the DIO model.
authors: Depto. de Cirugía, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico Depto. de Cirugía Experimental, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Gonzalo
authors: G
authors: Torres-Villalobos
authors: Depto. de Cirugía Experimental, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Nashla
authors: N
authors: Hamdan-Pérez
authors: Depto. de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors: Andrea
authors: A
authors: Díaz-Villaseñor
authors: Depto. de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Armando R
authors: AR
authors: Tovar
authors: Depto. de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Ivan
authors: I
authors: Torre-Villalvazo
authors: Depto. de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Guillermo
authors: G
authors: Ordaz-Nava
authors: Depto. de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Sofía
authors: S
authors: Morán-Ramos
authors: Depto. de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Lilia G
authors: LG
authors: Noriega
authors: Depto. de Patología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Braulio
authors: B
authors: Martínez-Benítez
authors: Depto. de Cirugía Experimental, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Alejandro
authors: A
authors: López-Garibay
authors: Depto. de Cirugía Experimental, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Samuel
authors: S
authors: Torres-Landa
authors: Depto. de Cirugía Experimental, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Juan C
authors: JC
authors: Ceballos-Cantú
authors: Depto. de Nefrología y Metabolismo Mineral, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Claudia
authors: C
authors: Tovar-Palacio
authors: Depto. de Nefrología y Metabolismo Mineral, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico.
authors: Elizabeth
authors: E
authors: Figueroa-Juárez
authors: Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors: Marcia
authors: M
authors: Hiriart
authors: Departamento de Posgrado e Investigación, Instituto Politécnico Nacional, Escuela Superior de Medicina, Mexico City, Mexico.
authors: Roberto
authors: R
authors: Medina-Santillán
authors: Departamento de Posgrado e Investigación, Instituto Politécnico Nacional, Escuela Superior de Medicina, Mexico City, Mexico.
authors: Carmen
authors: C
authors: Castillo-Hernández
authors: Depto. de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (INCMNSZ), Tlalpan, Mexico City, Mexico nimbester@gmail.com.
authors: Nimbe
authors: N
authors: Torres
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D064592:Autografts; D000070601:Diet, Carbohydrate Loading / Q000009:adverse effects / Q000379:methods; D059305:Diet, High-Fat / Q000009:adverse effects* / Q000379:methods; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005234:Fatty Liver / Q000378:metabolism*; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009765:Obesity / Q000209:etiology / Q000378:metabolism* / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D050151:Subcutaneous Fat / Q000637:transplantation*
chemical_list: D005230:Fatty Acids, Nonesterified; D007328:Insulin
keywords: Autologous adipose tissue transplant; diet‐induced obesity; insulin resistance; obesity