title: Whole-Brain Microscopy Meets In Vivo Neuroimaging: Techniques, Benefits, and Limitations.
abstract: Magnetic resonance imaging, positron emission tomography, and optical imaging have emerged as key tools to understand brain function and neurological disorders in preclinical mouse models. They offer the unique advantage of monitoring individual structural and functional changes over time. What remained unsolved until recently was to generate whole-brain microscopy data which can be correlated to the 3D in vivo neuroimaging data. Conventional histological sections are inappropriate especially for neuronal tracing or the unbiased screening for molecular targets through the whole brain. As part of the European Society for Molecular Imaging (ESMI) meeting 2016 in Utrecht, the Netherlands, we addressed this issue in the Molecular Neuroimaging study group meeting. Presentations covered new brain clearing methods, light sheet microscopes for large samples, and automatic registration of microscopy to in vivo imaging data. In this article, we summarize the discussion; give an overview of the novel techniques; and discuss the practical needs, benefits, and limitations.
authors: In-vivo-NMR Laboratory, Max Planck Institute for Metabolism Research, Cologne, Germany.
authors: Markus
authors: M
authors: Aswendt
authors: Department of Epileptology, University of Bonn, Bonn, Germany.
authors: Martin
authors: M
authors: Schwarz
authors: Department of Radiology, Division of Image Processing, Leiden University Medical Center, Leiden, The Netherlands.
authors: Walid M
authors: WM
authors: Abdelmoula
authors: Department of Radiology, Division of Image Processing, Leiden University Medical Center, Leiden, The Netherlands.
authors: Jouke
authors: J
authors: Dijkstra
authors: Experimental Laboratory Translational Neurosciences and Otolaryngology, Department of Translational Neurosciences, University of Antwerp, Antwerp, Belgium. stefanie.dedeurwaerdere@uantwerpen.be.
authors: Stefanie
authors: S
authors: Dedeurwaerdere
mesh_terms: D000716:Anatomy, Artistic; D000818:Animals; D001271:Atlases as Topic; D001921:Brain / Q000033:anatomy & histology*; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000379:methods*; D059906:Neuroimaging / Q000379:methods*
keywords: Brain atlas; Brain clearing; Clarity; Image registration; In vivo neuroimaging; Light sheet microscopy; MRI; Pet