title: Utilization of ancillary studies in the cytologic diagnosis of respiratory lesions: The papanicolaou society of cytopathology consensus recommendations for respiratory cytology.
abstract: The Papanicolaou Society of Cytopathology has developed a set of guidelines for respiratory cytology including indications for sputum examination, bronchial washings and brushings, CT-guided FNA and endobronchial ultrasound guided fine needle aspiration (EBUS-FNA), as well as recommendations for classification and criteria, ancillary testing and post-cytologic diagnosis management and follow-up. All recommendation documents are based on the expertise of committee members, an extensive literature review, and feedback from presentations at national and international conferences. The guideline documents selectively present the results of these discussions. The present document summarizes recommendations for ancillary testing of cytologic samples. Ancillary testing including microbiologic, immunocytochemical, flow cytometric, and molecular testing, including next-generation sequencing are discussed. Diagn. Cytopathol. 2016;44:1000-1009. © 2016 Wiley Periodicals, Inc.
authors: Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, Missouri.
authors: Lester J
authors: LJ
authors: Layfield
authors: Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas.
authors: Sinchita
authors: S
authors: Roy-Chowdhuri
authors: Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.
authors: Zubair
authors: Z
authors: Baloch
authors: Department of Pathology, Fox Chase Cancer Center, Temple University, Philadelphia, Pennsylvania.
authors: Hormoz
authors: H
authors: Ehya
authors: Department of Pathology, University of Mississippi, Jackson, Mississippi.
authors: Kim
authors: K
authors: Geisinger
authors: Department of Pathology and Cell Biology, Columbia University Medical Center, New York, New York.
authors: Susan J
authors: SJ
authors: Hsiao
authors: Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
authors: Oscar
authors: O
authors: Lin
authors: Department of Pathology, Harvard Medical School, Boston, Massachusetts.
authors: Neal I
authors: NI
authors: Lindeman
authors: Department of Pathology, University of Michigan School of Medicine, Ann Arbor, Michigan.
authors: Michael
authors: M
authors: Roh
authors: Département de Médecine, Laboratoire National de Santé, Dudelange, Luxembourg.
authors: Fernando
authors: F
authors: Schmitt
authors: Department of Pathology and Laboratory Medicine, The University of Vermont, Burlington, Vermont.
authors: Nikoletta
authors: N
authors: Sidiropoulos
authors: Department of Pathology, Beth Israel Deaconess Medical Center, Boston, Massachusetts.
authors: Paul A
authors: PA
authors: VanderLaan
mesh_terms: D014408:Biomarkers, Tumor / Q000592:standards; D001999:Bronchoscopy / Q000592:standards; D002277:Carcinoma / Q000145:classification / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D061765:Endoscopic Ultrasound-Guided Fine Needle Aspiration / Q000592:standards; D006801:Humans; D065006:Papanicolaou Test / Q000592:standards; D010338:Pathology, Clinical / Q000458:organization & administration; D017410:Practice Guidelines as Topic; D012142:Respiratory Tract Neoplasms / Q000145:classification / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012955:Societies, Medical; D013183:Sputum / Q000166:cytology
chemical_list: D014408:Biomarkers, Tumor
keywords: cytology; flow cytometry; immunocytochemistry; molecular diagnostics; next-generation sequencing