title: Molecular variation analysis ofAspergillus flavususing polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer rDNA region.
abstract: Aspergillus flavusis the second most common disease-causing species ofAspergillusin humans. The fungus is frequently associated with life-threatening infections in immunocompromised hosts. The primary aim of the present study was to analyze the genetic variability among different isolates ofA. flavususing polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP). A total of 62A. flavusisolates were tested in the study. Molecular variability was searched for by analysis of the PCR amplification of the internal transcribed spacer (ITS) regions of ribosomal DNA using restriction enzymes. PCR using primers for ITS1 and ITS4 resulted in a product of ~600 bp. Amplicons were subjected to digestion with restriction endonucleasesEcoRI,HaeIII andTaqI. Digestion of the PCR products using these restriction enzymes produced different patterns of fragments among the isolates, with different sizes and numbers of fragments, revealing genetic variability. In conclusion, ITS-RFLP is a useful molecular tool in screening for nucleotide polymorphisms amongA. flavusisolates.
authors: Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan 61357-15794, Iran; Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan 61357-15794, Iran.
authors: Majid
authors: M
authors: Zarrin
authors: Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan 61357-15794, Iran.
authors: Maryam
authors: M
authors: Erfaninejad
keywords: Aspergillus flavus; internal transcribed spacer rDNA region; polymerase chain reaction-restriction fragment length polymorphism