title: Bisphenol A induces spermatocyte apoptosis in rare minnow Gobiocypris rarus.
abstract: Bisphenol A (BPA) is an endocrine disruptor, and could induce germ cells apoptosis in the testis of mammals. But whether it could affect fish in the same mechanism has not' been studied till now. In the present study, to investigate the influence of BPA on testis germ cells in fish, adult male rare minnow Gobiocypris rarus were exposed to 225μgL(-1) (0.99μM) BPA for 1, 3 and 9 weeks. Through TdT-mediated dUTP nick end labeling (TUNEL) and transmission electron microscope (TEM) analysis, we found that the amount of apoptotic spermatocytes significantly increased in a time dependent manner following BPA exposure. Western Blot results showed that the ratio of Bcl2/Bax, the important apoptosis regulators in intrinsic mitochondrial apoptotic pathway, was significantly decreased. qPCR showed that mRNA expression of several genes in mitochondrial apoptotic pathway including bcl2, bax, casp9, cytc and mcl1b were significantly changed following BPA exposure. In addition, mRNA expression of meiosis regulation genes (kpna7 and wee2), and genes involved in both apoptosis and meiosis (birc5, ccna1, and gsa1a) were also affected by BPA. Taken together, the present study demonstrated that BPA could induce spermatocytes apoptosis in rare minnow testis, and the apoptosis was probably under regulation of intrinsic mitochondrial apoptotic pathway. Moreover, the spermatocyte apoptosis was likely initiated by BPA induced meiosis arrest.
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100 China.
authors: Yingying
authors: Y
authors: Zhang
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100 China.
authors: Mengqian
authors: M
authors: Cheng
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100 China.
authors: Lang
authors: L
authors: Wu
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100 China.
authors: Guo
authors: G
authors: Zhang
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100 China. Electronic address: zzwang@nwsuaf.edu.cn.
authors: Zaizhao
authors: Z
authors: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001559:Benzhydryl Compounds / Q000633:toxicity*; D015153:Blotting, Western; D003530:Cyprinidae / Q000254:growth & development / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity*; D008297:Male; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010636:Phenols / Q000633:toxicity*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D013090:Spermatocytes / Q000166:cytology* / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D051028:bcl-2-Associated X Protein; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Apoptosis; Bisphenol A; Meiosis arrest; Rare minnow Gobiocypris rarus; Spermatocyte; Testis