title: Epigenomic Regulation of Schwann Cell Reprogramming in Peripheral Nerve Injury.
abstract: The rapid and dynamic transcriptional changes of Schwann cells in response to injury are critical to peripheral nerve repair, yet the epigenomic reprograming that leads to the induction of injury-activated genes has not been characterized. Polycomb Repressive Complex 2 (PRC2) catalyzes the trimethylation of lysine 27 of histone H3 (H3K27me3), which produces a transcriptionally repressive chromatin environment. We find that many promoters and/or gene bodies of injury-activated genes of mature rat nerves are occupied with H3K27me3. In contrast, the majority of distal enhancers that gain H3K27 acetylation after injury are not repressed by H3K27 methylation before injury, which is normally observed in developmentally poised enhancers. Injury induces demethylation of H3K27 in many genes, such as Sonic hedgehog (Shh), which is silenced throughout Schwann cell development before injury. In addition, experiments using a Schwann cell-specific mouse knock-out of the Eed subunit of PRC2 indicate that demethylation is a rate-limiting step in the activation of such genes. We also show that some transcription start sites of H3K27me3-repressed injury genes of uninjured nerves are bound with a mark of active promoters H3K4me3, for example, Shh and Gdnf, and the reduction of H3K27me3 results in increased trimethylation of H3K4. Our findings identify reversal of polycomb repression as a key step in gene activation after injury.
  
  
  
  Peripheral nerve regeneration after injury is dependent upon implementation of a novel genetic program in Schwann cells that supports axonal survival and regeneration. Identifying means to enhance Schwann cell reprogramming after nerve injury could be used to foster effective remyelination in the treatment of demyelinating disorders and in identifying pathways involved in regenerative process of myelination. Although recent progress has identified transcriptional determinants of successful reprogramming of the Schwann cell transcriptome after nerve injury, our results have highlighted a novel epigenomic pathway in which reversal of the Polycomb pathway of repressive histone methylation is required for activation of a significant number of injury-induced genes.
authors: Waisman Center, Cellular and Molecular Pathology Graduate Program, and.
authors: Ki H
authors: 0000-0003-3474-7287
authors: KH
authors: Ma
authors: Waisman Center, Cellular and Molecular Pathology Graduate Program, and.
authors: Holly A
authors: 0000-0001-6668-2591
authors: HA
authors: Hung
authors: Waisman Center, Department of Comparative Biosciences, University of Wisconsin, Madison, Wisconsin 53705 john.svaren@wisc.edu.
authors: John
authors: 0000-0003-2963-7921
authors: J
authors: Svaren
mesh_terms: D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D065150:Cellular Reprogramming / Q000235:genetics / Q000502:physiology*; D047369:Chromatin Immunoprecipitation; D019295:Computational Biology; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology; D057890:Epigenomics; D005786:Gene Expression Regulation / Q000502:physiology*; D006657:Histones / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D063151:Polycomb Repressive Complex 2 / Q000235:genetics / Q000378:metabolism; D011743:Pyrimidines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000235:genetics; D012583:Schwann Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D020426:Sciatic Neuropathy / Q000378:metabolism* / Q000503:physiopathology*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D001552:Benzazepines; C107275:Eed protein, mouse; D004791:Enzyme Inhibitors; C000593030:GSK-J4; D006657:Histones; D011743:Pyrimidines; D012333:RNA, Messenger; D056484:Jumonji Domain-Containing Histone Demethylases; C523761:Kdm6b protein, mouse; D063151:Polycomb Repressive Complex 2
keywords: Schwann cell; chromatin; injury; myelin; polycomb; regeneration