title: Preparation of a thermoresponsive polymer grafted polystyrene monolithic capillary for the separation of bioactive compounds.
abstract: To develop aqueous microseparation columns for bioactive compounds, a thermoresponsive polymer grafted polymer monolith was prepared inside silica capillaries having an I.D. of 100μm by polymerization of styrene (St) with m/p-divinylbenzene (DVB) in the presence of polydimethylsiloxane as porogen, followed by surface-initiated atom transfer radical polymerization (SI-ATRP) of N-isopropylacrylamide (NIPAAm). SEM analysis indicated that the resulting poly(N-isopropylacrylamide) (PNIPAAm) grafted polystyrene monolith had a consecutive three-dimensionally interconnected structure and through-pores, similar to the base polystyrene (PSt) monolith. The elution behavior of steroids with different hydrophobicity was evaluated using micro-high-performance liquid chromatography in sole aqueous mobile phase. Temperature dependent interaction changes were observed between steroids and the PNIPAAm modified surfaces. Furthermore, the interaction between bioactive compounds and the PNIPAAm grafted PSt surfaces was controlled and eventually separate these molecules with different hydrophobicities by simple temperature modulation in aqueous environment. The PNIPAAm grafted PSt monolithic capillary showed improved separation properties of bioactive compounds, compared with a PNIPAAm grafted hollow capillary in aqueous environment.
authors: Department of Materials Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan. Electronic address: 8214701@ed.tus.ac.jp.
authors: Takuya
authors: T
authors: Koriyama
authors: Department of Materials Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan; The OCU Advanced Research Institute for Natural Science and Technology, Osaka City University, 3-3-138 Sugimotocho, Sumiyoshi-ku, Osaka, Osaka 558-8585, Japan. Electronic address: asoh@sci.osaka-cu.ac.jp.
authors: Taka-Aki
authors: TA
authors: Asoh
authors: Department of Materials Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan. Electronic address: kikuchia@rs.noda.tus.ac.jp.
authors: Akihiko
authors: A
authors: Kikuchi
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification*; D013499:Surface Properties; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D000178:Acrylamides; D011108:Polymers; D011137:Polystyrenes; D013256:Steroids; D014867:Water; D012822:Silicon Dioxide
keywords: Capillary column; Poly(N-isopropylacrylamide); Polymer monolith; SI-ATRP; Thermoresponsive chromatography