title: Development of a murine model of traumatic benign paroxysmal positional vertigo: a preliminary study.
abstract: CONCLUSIONS
  The results showed a gradual detachment of otoconia in the utricle after a single event of head vibration, possibly explaining the frequent recurrence of BPPV attacks and persistent dizziness after trauma.
  
  
  OBJECTIVE
  This study developed a murine model of traumatic BPPV and observed the changes in otoconia detachment over time.
  
  
  METHODS
  Six-week-old CBA mice were used in this study. Otoconia detachment was induced by vibrating the head for 2 min. Utricles of mice were harvested from different groups: before the head vibration and 1 day, 1 week, 1 month, and 3 months after vibration application. Using scanning electron microscopy and ImageJ software, the percentage of the intact area of otoconia in the utricle was calculated. Hearing thresholds were compared among the groups.
  
  
  RESULTS
  The mean (± SD) percentages of the intact area of otoconia in the utricle were 98.1% ± 1.7% before the vibration and 93.6% ± 1.7%, 88.9% ± 5.3%, 78.2% ± 20.9%, and 38.9% ± 24.1% at 1 day, 1 week, 1 month, and 3 months after the vibration, respectively. The percentage decreased significantly over time after the vibration (p < .001). The hearing thresholds were not different among the groups.
authors: a Department of Otolaryngology , Asan Medical Center, University of Ulsan College of Medicine , Seoul , Korea.
authors: Chan Joo
authors: CJ
authors: Yang
authors: b Asan Institute for Life Science, Asan Medical Center, University of Ulsan College of Medicine , Seoul , Korea.
authors: Ji-Won
authors: JW
authors: Lee
authors: b Asan Institute for Life Science, Asan Medical Center, University of Ulsan College of Medicine , Seoul , Korea.
authors: Sung-Jin
authors: SJ
authors: Kim
authors: b Asan Institute for Life Science, Asan Medical Center, University of Ulsan College of Medicine , Seoul , Korea.
authors: Chang-Whan
authors: CW
authors: Lee
authors: a Department of Otolaryngology , Asan Medical Center, University of Ulsan College of Medicine , Seoul , Korea.
authors: Hong Ju
authors: HJ
authors: Park
mesh_terms: D000818:Animals; D001309:Auditory Threshold; D065635:Benign Paroxysmal Positional Vertigo; D004195:Disease Models, Animal; D008297:Male; D008808:Mice, Inbred CBA; D010037:Otolithic Membrane / Q000473:pathology; D014732:Vibration
keywords: Benign paroxysmal positional vertigo; murine model; otoconia