title: PLGA nano/micro particles encapsulated with pertussis toxoid (PTd) enhances Th1/Th17 immune response in a murine model.
abstract: Poly(lactic-co-glycolic acid) (PLGA) based nano/micro particles were investigated as a potential vaccine platform for pertussis antigen. Presentation of pertussis toxoid as nano/micro particles (NP/MP) gave similar antigen-specific IgG responses in mice compared to soluble antigen. Notably, in cell line based assays, it was found that PLGA based nano/micro particles enhanced the phagocytosis of fluorescent antigen-nano/micro particles by J774.2 murine monocyte/macrophage cells compared to soluble antigen. More importantly, when mice were immunised with the antigen-nano/micro particles they significantly increased antigen-specific Th1 cytokines INF-γ and IL-17 secretion in splenocytes after in vitro re-stimulation with heat killed Bordetalla pertussis, indicating the induction of a Th1/Th17 response. Also, presentation of pertussis antigen in a NP/MP formulation is able to provide protection against respiratory infection in a murine model. Thus, the NP/MP formulation may provide an alternative to conventional acellular vaccines to achieve a more balanced Th1/Th2 immune response.
authors: Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Biomaterial Research, Tianjin 300192, China.
authors: Pan
authors: P
authors: Li
authors: Division of Bacteriology, National Institute for Biological Standards and Control (NIBSC), Blanche Lane, South Mimms, Hertfordshire, EN6 3QG, UK.
authors: Catpagavalli
authors: C
authors: Asokanathan
authors: Department of Pharmacy, Pharmacology and Postgraduate Medicine, University of Hertfordshire, Hatfield, AL10 9AB, UK. Electronic address: f.liu3@herts.ac.uk.
authors: Fang
authors: F
authors: Liu
authors: Department of Pharmacy, Pharmacology and Postgraduate Medicine, University of Hertfordshire, Hatfield, AL10 9AB, UK.
authors: Kyi Kyi
authors: KK
authors: Khaing
authors: Division of Bacteriology, National Institute for Biological Standards and Control (NIBSC), Blanche Lane, South Mimms, Hertfordshire, EN6 3QG, UK.
authors: Dorota
authors: D
authors: Kmiec
authors: Cardiff Institute of Tissue Engineering & Repair, School of Dentistry, Collegeof Biomedical and Life Sciences, Cardiff University, UK.
authors: Xiaoqing
authors: X
authors: Wei
authors: Cardiff Institute of Tissue Engineering & Repair, School of Dentistry, Collegeof Biomedical and Life Sciences, Cardiff University, UK.
authors: Bing
authors: B
authors: Song
authors: Division of Bacteriology, National Institute for Biological Standards and Control (NIBSC), Blanche Lane, South Mimms, Hertfordshire, EN6 3QG, UK.
authors: Dorothy
authors: D
authors: Xing
authors: Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Biomaterial Research, Tianjin 300192, China. Electronic address: kongdeling@hotmail.com.
authors: Deling
authors: D
authors: Kong
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001886:Bordetella pertussis / Q000276:immunology; D002460:Cell Line; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D020381:Interleukin-17 / Q000276:immunology; D019344:Lactic Acid / Q000737:chemistry*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008863:Microspheres; D009000:Monocytes / Q000276:immunology; D053758:Nanoparticles; D010587:Phagocytosis / Q000276:immunology; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013154:Spleen / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D014121:Toxoids / Q000008:administration & dosage* / Q000276:immunology; D014917:Whooping Cough / Q000517:prevention & control
chemical_list: D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D020381:Interleukin-17; D014121:Toxoids; C051093:pertussis toxoid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D007371:Interferon-gamma
keywords: Adjuvant; PLGA nano/micro particles; Pertussis; Phagocytosis