title: Apolipoprotein L Expression Correlates with Neutrophil Cell Death in Critically Ill Patients.
abstract: Delayed neutrophil apoptosis has been demonstrated in sepsis and may contribute to organ damage. It has recently been proposed that apolipoprotein L (ApoL) may be involved in programmed cell death, but the expression and functions of ApoLs in leukocytes (especially neutrophils) during sepsis and other inflammatory conditions are currently unknown. In this prospective observational study in a 36-bed university hospital medicosurgical intensive care unit (ICU), we included 78 adult ICU patients with (n = 41) or without (n = 37) sepsis and 47 healthy volunteers. We analyzed ApoL mRNA expression using quantitative polymerase chain reaction in whole blood leukocytes and protein expression in CD15 isolated neutrophils using Western blotting. Neutrophil apoptosis was assessed using the APO-BRDU method. Apolipoprotein L mRNA was downregulated in whole blood leukocytes and neutrophils in ICU patients compared with in healthy volunteers, and this effect translated at the protein level as indicated by Western blot analysis of neutrophils. There was a negative correlation between ApoL expression in neutrophils and C-reactive protein levels and a positive correlation between the number of apoptotic neutrophils and mRNA levels of ApoL1 and ApoL2. The degree of neutrophil apoptosis in critically ill patients is therefore correlated with modified expression profiles of ApoLs.
authors: *Laboratoire de Médecine Expérimentale (ULB 222 Unit), Université Libre de Bruxelles, CHU de Charleroi, A. Vésale Hospital, Montigny-Le-Tilleul, Belgium†Laboratory of Cancer Biology and Molecular Immunology, Lebanese University, Faculty of Sciences, Hadath-Beirut, Lebanon‡Department of Intensive Care, Erasme University Hospital, Université libre de Bruxelles, Brussels, Belgium§Institute for Molecular Biology and Medicine (IBMM), Université libre de Bruxelles, Gosselies, Belgium.
authors: Israa
authors: I
authors: Akl
authors: Christophe
authors: C
authors: Lelubre
authors: Pierrick
authors: P
authors: Uzureau
authors: Michael
authors: M
authors: Piagnerelli
authors: Patrick
authors: P
authors: Biston
authors: Alexandre
authors: A
authors: Rousseau
authors: Bassam
authors: B
authors: Badran
authors: Hussein
authors: H
authors: Fayyad-Kazan
authors: Mohammad
authors: M
authors: Ezedine
authors: Jean-Louis
authors: JL
authors: Vincent
authors: Karim Zouaoui
authors: KZ
authors: Boudjeltia
authors: Luc
authors: L
authors: Vanhamme
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000075944:Apolipoprotein L1 / Q000378:metabolism*; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D002097:C-Reactive Protein / Q000378:metabolism; D016923:Cell Death / Q000235:genetics / Q000502:physiology*; D016638:Critical Illness; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007362:Intensive Care Units / Q000706:statistics & numerical data; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism*; D011446:Prospective Studies; D018805:Sepsis / Q000378:metabolism
chemical_list: D000075944:Apolipoprotein L1; D002097:C-Reactive Protein