title: Oil/water nano-emulsion loaded with cobalt ferrite oxide nanocubes for photo-acoustic and magnetic resonance dual imaging in cancer: in vitro and preclinical studies.
abstract: Dual imaging dramatically improves detection and early diagnosis of cancer. In this work we present an oil in water (O/W) nano-emulsion stabilized with lecithin and loaded with cobalt ferrite oxide (Co0.5Fe2.5O4) nanocubes for photo-acoustic and magnetic resonance dual imaging. The nanocarrier is responsive in in vitro photo-acoustic and magnetic resonance imaging (MRI) tests. A clear and significant time-dependent accumulation in tumor tissue is shown in in vivo photo-acoustic studies on a murine melanoma xenograft model. The proposed O/W nano-emulsion exhibits also high values of r2/r1(ranging from 45 to 85, depending on the magnetic field) suggesting a possible use as T2weighted image contrast agents. In addition, viability and cellular uptake studies show no significant cytotoxicity on the fibroblast cell line. We also tested the O/W nano-emulsion loaded with curcumin against melanoma cancer cells demonstrating a significant cytotoxicity and thus showing possible therapeutic effects in addition to the in vivo imaging.
authors: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy; Interdisciplinary Research Center of Biomaterials, CRIB, University Federico II, P.le Tecchio, Naples, Italy.
authors: Raffaele
authors: R
authors: Vecchione
authors: Medical Oncology, Abdominal Department, National Cancer Institute G. Pascale Foundation, Naples, Italy; ASMO (Association for Multidisciplinary Studies in Oncology) and Mediterranean Diet, Naples, Italy.
authors: Vincenzo
authors: V
authors: Quagliariello
authors: Center for Preclinical Imaging, Department of Molecular Biotechnology and Health Sciences, Molecular Imaging Center, University of Turin, Italy.
authors: Pierangela
authors: P
authors: Giustetto
authors: Interdisciplinary Research Center of Biomaterials, CRIB, University Federico II, P.le Tecchio, Naples, Italy.
authors: Dominic
authors: D
authors: Calabria
authors: Istituto Italiano di Tecnologia, Genova, Italy.
authors: Ayyappan
authors: A
authors: Sathya
authors: Istituto Italiano di Tecnologia, Genova, Italy.
authors: Roberto
authors: R
authors: Marotta
authors: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy; Interdisciplinary Research Center of Biomaterials, CRIB, University Federico II, P.le Tecchio, Naples, Italy.
authors: Martina
authors: M
authors: Profeta
authors: Istituto Italiano di Tecnologia, Genova, Italy.
authors: Simone
authors: S
authors: Nitti
authors: Istituto Italiano di Tecnologia, Genova, Italy.
authors: Niccolò
authors: N
authors: Silvestri
authors: Istituto Italiano di Tecnologia, Genova, Italy.
authors: Teresa
authors: T
authors: Pellegrino
authors: Medical Oncology, Abdominal Department, National Cancer Institute G. Pascale Foundation, Naples, Italy; ASMO (Association for Multidisciplinary Studies in Oncology) and Mediterranean Diet, Naples, Italy. Electronic address: r.iaffaioli@istitutotumori.na.it.
authors: Rosario V
authors: RV
authors: Iaffaioli
authors: Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Naples, Italy; Interdisciplinary Research Center of Biomaterials, CRIB, University Federico II, P.le Tecchio, Naples, Italy. Electronic address: paolo.netti@iit.it.
authors: Paolo Antonio
authors: PA
authors: Netti
mesh_terms: D016475:3T3 Cells; D000818:Animals; D003035:Cobalt / Q000737:chemistry*; D003287:Contrast Media; D004655:Emulsions / Q000737:chemistry; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008545:Melanoma / Q000000981:diagnostic imaging*; D008546:Melanoma, Experimental; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D009368:Neoplasm Transplantation; D010087:Oxides / Q000737:chemistry; D061088:Photoacoustic Techniques
chemical_list: D003287:Contrast Media; D004655:Emulsions; D010087:Oxides; D003035:Cobalt
keywords: Cubic nanoparticles; Dual-imaging; MRI; Nano-emulsion; Photo-acoustic