title: Response of Stem and Progenitor Cells to Testicular Ischemia.
abstract: Stem and progenitor cells were studied on mouse model of testicular ischemia. Testicular ischemia led to a decrease in free testosterone concentration. Hemodynamic changes, interstitial edema, and destruction of spermatogenic epithelium, Leydig, and Sertoli cells were observed in the testicular tissue. Accumulation of degenerative germ cells was accompanied by reduction in the count of spermatogonial stem cells with immunophenotype CD117(-)CD29(+)CD90(+) and CD117(+)CD29(+)CD90(+). Simultaneously with pathomorphological changes in the testes and suppression of spermatogenesis, ischemia reduced the count of hematopoietic progenitor cells, hematopoietic stem cells with immunophenotype Lin(-)CD117(+)Sca-1(+)c-kit(+)CD34(+) and Lin(-)CD117(+)Sca-1(+)c-kit(+)CD34(-), and multipotent mesenchymal stromal cells (CD45(-)CD31(-) CD90(+)CD106(+)) in the testicular tissue. The population of CD45(-)CD31(+)-endothelial cells in ischemic testicular tissue increased.
authors: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors: E G
authors: EG
authors: Skurikhin
authors: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia. angelinapakhomova2011@gmail.com.
authors: A V
authors: AV
authors: Pakhomova
authors: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors: N N
authors: NN
authors: Ermakova
authors: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors: O V
authors: OV
authors: Pershina
authors: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors: E S
authors: ES
authors: Pan
authors: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors: L A
authors: LA
authors: Ermolaeva
authors: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors: A I
authors: AI
authors: Kudryashova
authors: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors: V A
authors: VA
authors: Krupin
authors: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors: O Yu
authors: OY
authors: Rybalkina
authors: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors: A M
authors: AM
authors: Dygai
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D000950:Antigens, Ly / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology*; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000473:pathology*; D007511:Ischemia / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D013737:Testis / Q000166:cytology* / Q000378:metabolism / Q000473:pathology*
chemical_list: D018952:Antigens, CD34; D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D019009:Proto-Oncogene Proteins c-kit
keywords: endothelial cells; hematopoietic stem cells; multipotent mesenchymal stromal cells; spermatogonial stem cells; testicular ischemia