title: Comprehensive profiling and characterization of cellular miRNAs in response to hepatitis A virus infection in human fibroblasts.
abstract: Hepatitis A virus (HAV), the causative agent of acute hepatitis, grows slowly without causing any cytopathic effect (CPE) and lead to a persistent infection in the fibroblasts in vitro. miRNAs play a key role in the viral pathogenesis and virus-host interactions. In this study, the comprehensive miRNA expression profiles of HAV-infected and uninfected fibroblasts were investigated by sRNA-seq and validated by RT-qPCR. The results showed that a total of 94 miRNAs were differentially expressed during HAV infection, including 11 up-regulated miRNAs and 83 down-regulated miRNAs. RT-qPCR analysis showed the expression levels of specific miRNAs were consistent with sRNA-seq data. Further, target prediction analysis showed 729 putative target genes that included many immune-related transcripts were revealed. The GO enrichment analysis and the KEGG pathway analysis of the target genes showed that various biological pathways, including JAK-STAT cascade, type I interferon signaling pathway could be affected by HAV infection by the alteration of host miRNAs. The core regulatory relationship between miRNAs and their targets were revealed by miRNA-gene-network. Collectively, this study provides an overall analysis of miRNA profile in cell culture infected with HAV. The present results imply the alteration of miRNAs expression induced by HAV infection which may be related to the establishment of persistent HAV infection and might provide new clues for understanding the persistent HAV infections in vitro and the unique biological characteristics associated with HAV during infection.
authors: Department of Vaccine Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China; Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China.
authors: Jiandong
authors: J
authors: Shi
authors: Department of Vaccine Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China; Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China.
authors: Jing
authors: J
authors: Sun
authors: Department of Vaccine Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China; Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China.
authors: Meini
authors: M
authors: Wu
authors: Department of Vaccine Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China; Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China.
authors: Haixuan
authors: H
authors: Wang
authors: Department of Vaccine Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China; Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China.
authors: Ningzhu
authors: N
authors: Hu
authors: Department of Vaccine Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China; Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China. Electronic address: huyunzhangym@126.com.
authors: Yunzhang
authors: Y
authors: Hu
mesh_terms: D002478:Cells, Cultured; D016000:Cluster Analysis; D005347:Fibroblasts / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006506:Hepatitis A / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D030041:Hepatitis A virus; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D017423:Sequence Analysis, RNA
chemical_list: D035683:MicroRNAs
keywords: Expression profile; Fibroblast; Hepatitis A virus; miRNA; sRNA-seq