title: Profiling Carbonylated Proteins in Heart and Skeletal Muscle Mitochondria from Trained and Untrained Mice.
abstract: Understanding the relationship between physical exercise, reactive oxygen species, and skeletal muscle modification is important in order to better identify the benefits or the damages that appropriate or inappropriate exercise can induce. Heart and skeletal muscles have a high density of mitochondria with robust energetic demands, and mitochondria plasticity has an important role in both the cardiovascular system and skeletal muscle responses. The aim of this study was to investigate the influence of regular physical activity on the oxidation profiles of mitochondrial proteins from heart and tibialis anterior muscles. To this end, we used the mouse as animal model. Mice were divided into two groups: untrained and regularly trained. The carbonylated protein pattern was studied by two-dimensional gel electrophoresis followed by Western blot with anti-dinitrophenyl hydrazone antibodies. Mass spectrometry analysis allowed the identification of several different protein oxidation sites, including methionine, cysteine, proline, and leucine residues. A large number of oxidized proteins were found in both untrained and trained animals. Moreover, mitochondria from skeletal muscles and heart showed almost the same carbonylation pattern. Interestingly, exercise training seems to increase the carbonylation level mainly of mitochondrial proteins from skeletal muscle.
authors: Department of Chemical Sciences, Federico II University, Complesso Universitario Monte Sant'Angelo , Via Cinthia 4, 80126 Naples, Italy.
authors: Andrea
authors: A
authors: Carpentieri
authors: Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence , Viale G.B. Morgagni 50, Florence, 50134 Italy.
authors: Tania
authors: T
authors: Gamberi
authors: Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence , Viale G.B. Morgagni 50, Florence, 50134 Italy.
authors: Alessandra
authors: A
authors: Modesti
authors: Department of Chemical Sciences, Federico II University, Complesso Universitario Monte Sant'Angelo , Via Cinthia 4, 80126 Naples, Italy.
authors: Angela
authors: A
authors: Amoresano
authors: Department of Experimental and Clinical Medicine, University of Florence , Viale G.B. Morgagni 63, 50134 Florence, Italy.
authors: Barbara
authors: B
authors: Colombini
authors: Department of Experimental and Clinical Medicine, University of Florence , Viale G.B. Morgagni 63, 50134 Florence, Italy.
authors: Marta
authors: M
authors: Nocella
authors: Department of Experimental and Clinical Medicine, University of Florence , Viale G.B. Morgagni 63, 50134 Florence, Italy.
authors: Maria Angela
authors: MA
authors: Bagni
authors: Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence , Viale G.B. Morgagni 50, Florence, 50134 Italy.
authors: Tania
authors: T
authors: Fiaschi
authors: Department of Chemical Sciences, Federico II University, Complesso Universitario Monte Sant'Angelo , Via Cinthia 4, 80126 Naples, Italy.
authors: Lorenzo
authors: L
authors: Barolo
authors: Department of Experimental and Clinical Medicine, University of Florence , Viale G.B. Morgagni 63, 50134 Florence, Italy.
authors: Massimo
authors: M
authors: Gulisano
authors: Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence , Viale G.B. Morgagni 50, Florence, 50134 Italy.
authors: Francesca
authors: F
authors: Magherini
mesh_terms: D000818:Animals; D015153:Blotting, Western; D051379:Mice; D008931:Mitochondria, Muscle / Q000737:chemistry*; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000378:metabolism; D009206:Myocardium / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010805:Physical Conditioning, Animal; D050050:Protein Carbonylation
chemical_list: D024101:Mitochondrial Proteins
keywords: exercise; mice; oxyblot; protein carbonylation; protein oxidation; reactive oxygen species (ROS); redox proteomics; tandem mass spectrometry (MS/MS); training; two-dimensional gel electrophoresis (2-DE)