title: Identification of linear B-cell epitopes on goose parvovirus non-structural protein.
abstract: Goose parvovirus (GPV) infection can cause a highly contagious and lethal disease in goslings and muscovy ducklings which is widespread in all major goose (Anser anser) and Muscovy duck (Cairina moschata) farming countries, leading to a huge economic loss. Humoral immune responses play a major role in GPV immune protection during GPV infection. However, it is still unknown for the localization and immunological characteristics of B-cell epitopes on GPV non-structural protein (NSP). Therefore, in this study, the epitopes on the NSP of GPV were identified by means of overlapping peptides expressed in Escherichia coli in combination with Western blot. The results showed that the antigenic epitopes on the GPV NSP were predominantly localized in the C-terminal (aa 485-627), and especially, the fragment NS (498-532) was strongly positive. These results may facilitate future investigations on the function of NSP of GPV and the development of immunoassays for the diagnosis of GPV infection.
authors: College of Veterinary Medicine, Northeast Agriculture University, Harbin 150030, PR China; College of Life Science and Agriculture Forestry, Qiqihar University, Qiqihar 161006, PR China.
authors: Tian-Fei
authors: TF
authors: Yu
authors: College of Veterinary Medicine, Northeast Agriculture University, Harbin 150030, PR China.
authors: Bo
authors: B
authors: Ma
authors: College of Veterinary Medicine, Northeast Agriculture University, Harbin 150030, PR China. Electronic address: jwwang@neau.edu.cn.
authors: Jun-Wei
authors: JW
authors: Wang
mesh_terms: D000818:Animals; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D005777:Geese / Q000821:virology*; D017992:Parvovirus / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: D018985:Epitopes, B-Lymphocyte; D010446:Peptide Fragments; D017361:Viral Nonstructural Proteins
keywords: B-cell epitopes; Goose parvovirus; Non-structural protein