title: Transcriptome Profiling and In Silico Analysis of the Antimicrobial Peptides of the GrasshopperOxya chinensis sinuosa.
abstract: Antimicrobial peptides/proteins (AMPs) are present in all types of organisms, from microbes and plants to vertebrates and invertebrates such as insects. The grasshopperOxya chinensis sinuosais an insect species that is widely consumed around the world for its broad medicinal value. However, the lack of available genetic information for this species is an obstacle to understanding the full potential of its AMPs. Analysis of theO. chinensis sinuosatranscriptome and expression profile is essential for extending the available genetic information resources. In this study, we determined the whole-body transcriptome ofO. chinensis sinuosaand analyzed the potential AMPs induced by bacterial immunization. A high-throughput RNA-Seq approach generated 94,348 contigs and 66,555 unigenes. Of these unigenes, 36,032 (54.14%) matched known proteins in the NCBI database in a BLAST search. Functional analysis demonstrated that 38,219 unigenes were clustered into 5,499 gene ontology terms. In addition, 26 cDNAs encoding novel AMPs were identified by an in silico approach using public databases. Our transcriptome dataset and AMP profile greatly improve our understanding ofO. chinensis sinuosagenetics and provide a huge number of gene sequences for further study, including genes of known importance and genes of unknown function.
authors: Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
authors: In-Woo
authors: IW
authors: Kim
authors: Theragen ETEX Bio Institute, Theragen Etex Inc., Suwon 16229, Republic of Korea.
authors: Kesavan
authors: K
authors: Markkandan
authors: Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
authors: Joon Ha
authors: JH
authors: Lee
authors: Theragen ETEX Bio Institute, Theragen Etex Inc., Suwon 16229, Republic of Korea.
authors: Sathiyamoorthy
authors: S
authors: Subramaniyam
authors: Theragen ETEX Bio Institute, Theragen Etex Inc., Suwon 16229, Republic of Korea.
authors: Seungil
authors: S
authors: Yoo
authors: Theragen ETEX Bio Institute, Theragen Etex Inc., Suwon 16229, Republic of Korea.
authors: Junhyung
authors: J
authors: Park
authors: Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
authors: Jae Sam
authors: JS
authors: Hwang
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003198:Computer Simulation; D020869:Gene Expression Profiling; D006110:Grasshoppers / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D023181:Antimicrobial Cationic Peptides; D019476:Insect Proteins
keywords: Grasshopper; Insect-derived AMP; Oxya chinensis; antimicrobial peptides; transcriptome