title: The metabolites of Ambinine, a benzo[c]phenanthridine alkaloid, in rats identified by ultra-performance liquid chromatography-quadrupoletime-of-flight mass spectrometry (UPLC/Q-TOF-MS/MS).
abstract: Ambinine (AM), one of the major hexahydrobenzo[c]phenanthridine alkaloids from Corydalis ambigua Cham. et Schlecht. var. amurensis Maxim, is considered to be an important compound because of its special content and activity. However, there are few published studies on AM metabolism. In this research, the metabolism of AM in vivo was comprehensively studied for the first time. A total 44 metabolites (including 13 phase I and 31 phase II metabolites) as well as its parent drug in plasma, bile, urine and feces of rats were identified and 41 of them were reported for the first time. The results obtained indicated that demethylation, sulfation and glucuronidation were the major metabolic pathways of AM in vivo. Furthermore, this study provides valuable and new information about the metabolism of AM, which will be very helpful for understanding the safety and efficacy of AM, as well as its analogues.
authors: School of Traditional Chinese MateriaMedica, Shenyang Pharmaceutical University, Shenyang 110016, China; Development and Utilization Key Laboratory of Northeast Plant Materials, Key Laboratory of Northeast Authentic Materials Research and Development in Liaoning Province, School of Traditional Chinese MateriaMedica, Shenyang Pharmaceutical University, Shenyang, China.
authors: Zhihui
authors: Z
authors: Liu
authors: School of Traditional Chinese MateriaMedica, Shenyang Pharmaceutical University, Shenyang 110016, China; Development and Utilization Key Laboratory of Northeast Plant Materials, Key Laboratory of Northeast Authentic Materials Research and Development in Liaoning Province, School of Traditional Chinese MateriaMedica, Shenyang Pharmaceutical University, Shenyang, China.
authors: Sheng
authors: S
authors: Chang
authors: School of Traditional Chinese MateriaMedica, Shenyang Pharmaceutical University, Shenyang 110016, China; Development and Utilization Key Laboratory of Northeast Plant Materials, Key Laboratory of Northeast Authentic Materials Research and Development in Liaoning Province, School of Traditional Chinese MateriaMedica, Shenyang Pharmaceutical University, Shenyang, China.
authors: Xiaoying
authors: X
authors: Guan
authors: School of Traditional Chinese MateriaMedica, Shenyang Pharmaceutical University, Shenyang 110016, China; Development and Utilization Key Laboratory of Northeast Plant Materials, Key Laboratory of Northeast Authentic Materials Research and Development in Liaoning Province, School of Traditional Chinese MateriaMedica, Shenyang Pharmaceutical University, Shenyang, China.
authors: Na
authors: N
authors: Han
authors: School of Traditional Chinese MateriaMedica, Shenyang Pharmaceutical University, Shenyang 110016, China; Development and Utilization Key Laboratory of Northeast Plant Materials, Key Laboratory of Northeast Authentic Materials Research and Development in Liaoning Province, School of Traditional Chinese MateriaMedica, Shenyang Pharmaceutical University, Shenyang, China. Electronic address: yinjun2002@yahoo.com.
authors: Jun
authors: J
authors: Yin
mesh_terms: D000470:Alkaloids / Q000097:blood / Q000737:chemistry / Q000378:metabolism* / Q000652:urine; D000818:Animals; D053119:Benzophenanthridines / Q000737:chemistry / Q000378:metabolism*; D001646:Bile / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry; D008297:Male; D050217:Metabolic Detoxication, Phase II; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D010617:Phenanthridines / Q000097:blood / Q000737:chemistry / Q000378:metabolism* / Q000652:urine; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000470:Alkaloids; D053119:Benzophenanthridines; D010617:Phenanthridines; C046987:ambinine
keywords: Ambinine; Metabolites; UPLC-Q/TOFMS