title: Frealign: An Exploratory Tool for Single-Particle Cryo-EM.
abstract: Frealign is a software tool designed to process electron microscope images of single molecules and complexes to obtain reconstructions at the highest possible resolution. It provides a number of refinement parameters and options that allow users to tune their refinement to achieve specific goals, such as masking to classify selected regions within a particle, control over the refinement of specific alignment parameters to accommodate various data collection schemes, refinement of pseudosymmetric particles, and generation of initial maps. This chapter provides a general overview of Frealign functions and a more detailed guide to using Frealign in typical scenarios.
authors: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, United States. Electronic address: niko@grigorieff.org.
authors: N
authors: N
authors: Grigorieff
mesh_terms: D000465:Algorithms; D020285:Cryoelectron Microscopy / Q000295:instrumentation / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods / Q000706:statistics & numerical data*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods; D008958:Models, Molecular; D020652:Peptide Elongation Factor 2 / Q000648:ultrastructure; D012270:Ribosomes / Q000648:ultrastructure; D000072760:Single Molecule Imaging / Q000379:methods* / Q000706:statistics & numerical data; D012984:Software
chemical_list: D020652:Peptide Elongation Factor 2
keywords: Asymmetry; Classification; Contrast transfer function; High resolution; Masking; Refinement