title: Biomimetic surface patterning for long-term transmembrane access.
abstract: Here we present a planar patch clamp chip based on biomimetic cell membrane fusion. This architecture uses nanometer length-scale surface patterning to replicate the structure and function of membrane proteins, creating a gigaohm seal between the cell and a planar electrode array. The seal is generated passively during cell spreading, without the application of a vacuum to the cell surface. This interface can enable cell-attached and whole-cell recordings that are stable to 72 hours, and generates no visible damage to the cell. The electrodes can be very small (<5 μm) and closely packed, offering a high density platform for cellular measurement.
authors: Microsystems Laboratory, EPFL-STI-IMT-LMIS4, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
authors: Jules J
authors: JJ
authors: VanDersarl
authors: Microsystems Laboratory, EPFL-STI-IMT-LMIS4, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
authors: Philippe
authors: P
authors: Renaud
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology*; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology*; D008839:Microelectrodes; D018408:Patch-Clamp Techniques / Q000295:instrumentation*; D018000:Receptors, Peptide / Q000235:genetics / Q000378:metabolism; D012825:Silicon / Q000737:chemistry; D019076:Transgenes
chemical_list: C447150:ANTXR2 protein, human; D018000:Receptors, Peptide; D049452:Green Fluorescent Proteins; D012825:Silicon