title: Immunological Analyses of Leukemia Stem Cells.
abstract: Traditionally, the intracellular localization and expression levels of specific proteins in CML Leukemia stem cells (LSCs) have been evaluated by fluorescence immunohistochemistry (FIHC). More recently, Duolink(®) in situ PLA technology has opened up a new and more quantitative way to evaluate signal transduction, posttranslational modification, and protein-protein interaction at the single-stem-cell level. This novel methodology, which employs two antibody-based probes, has already increased our understanding of the biology of the rare CML LSC population. In the future, the use of this approach may contribute to the development of novel therapeutics aimed at eradicating CML LSCs in CML patients.
authors: Department of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan. kanaka55@hiroshima-u.ac.jp.
authors: Kazuhito
authors: K
authors: Naka
authors: Department of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan.
authors: Yoshihiro
authors: Y
authors: Takihara
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique / Q000379:methods*; D016044:Fusion Proteins, bcr-abl / Q000235:genetics / Q000276:immunology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D014411:Neoplastic Stem Cells / Q000378:metabolism*
chemical_list: D016044:Fusion Proteins, bcr-abl
keywords: Duolink® in situ PLA; Fluorescence immunohistochemistry (FIHC); Posttranslational modification; Protein-protein interaction; Single CML stem cell