title: Alternative Oxidase Gene Family in Hypericum perforatum L.: Characterization and Expression at the Post-germinative Phase.
abstract: Alternative oxidase (AOX) protein is located in the inner mitochondrial membrane and is encoded in the nuclear genome being involved in plant response upon a diversity of environmental stresses and also in normal plant growth and development. Here we report the characterization of the AOX gene family of Hypericum perforatum L. Two AOX genes were identified, both with a structure of four exons (HpAOX1, acc. KU674355 and HpAOX2, acc. KU674356). High variability was found at the N-terminal region of the protein coincident with the high variability identified at the mitochondrial transit peptide. In silico analysis of regulatory elements located at intronic regions identified putative sequences coding for miRNA precursors and trace elements of a transposon. Simple sequence repeats were also identified. Additionally, the mRNA levels for the HpAOX1 and HpAOX2, along with the ones for the HpGAPA (glyceraldehyde-3-phosphate dehydrogenase A subunit) and the HpCAT1 (catalase 1), were evaluated during the post-germinative development. Gene expression analysis was performed by RT-qPCR with accurate data normalization, pointing out HpHYP1 (chamba phenolic oxidative coupling protein 1) and HpH2A (histone 2A) as the most suitable reference genes (RGs) according to GeNorm algorithm. The HpAOX2 transcript demonstrated larger stability during the process with a slight down-regulation in its expression. Contrarily, HpAOX1 and HpGAPA (the corresponding protein is homolog to the chloroplast isoform involved in the photosynthetic carbon assimilation in other plant species) transcripts showed a marked increase, with a similar expression pattern between them, during the post-germinative development. On the other hand, the HpCAT1 (the corresponding protein is homolog to the major H2O2-scavenging enzyme in other plant species) transcripts showed an opposite behavior with a down-regulation during the process. In summary, our findings, although preliminary, highlight the importance to investigate in more detail the participation of AOX genes during the post-germinative development in H. perforatum, in order to explore their functional role in optimizing photosynthesis and in the control of reactive oxygen species (ROS) levels during the process.
authors: ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Laboratório de Biologia Molecular, Universidade de Évora Pólo da Mitra, Évora, Portugal.
authors: Isabel
authors: I
authors: Velada
authors: ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Laboratório de Biologia Molecular, Universidade de Évora Pólo da Mitra, Évora, Portugal.
authors: Hélia G
authors: HG
authors: Cardoso
authors: ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Laboratório de Biologia Molecular, Universidade de Évora Pólo da Mitra, Évora, Portugal.
authors: Carla
authors: C
authors: Ragonezi
authors: Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia-Universidade de Lisboa Lisboa, Portugal.
authors: Amaia
authors: A
authors: Nogales
authors: ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Laboratório de Biologia Molecular, Universidade de Évora Pólo da Mitra, Évora, Portugal.
authors: Alexandre
authors: A
authors: Ferreira
authors: ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Laboratório de Biologia Molecular, Universidade de Évora Pólo da Mitra, Évora, Portugal.
authors: Vera
authors: V
authors: Valadas
authors: EU Marie Curie Chair, ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora Pólo da Mitra, Évora, Portugal.
authors: Birgit
authors: B
authors: Arnholdt-Schmitt
keywords: St. John's Wort; alternative oxidase; cyanide-resistant pathway; gene expression; miRNAs; plant development; post-germination; transposable elements