title: Neonatal treatment with scopolamine butylbromide prevents metabolic dysfunction in male rats.
abstract: We tested whether treatment with a cholinergic antagonist could reduce insulin levels in early postnatal life and attenuate metabolic dysfunctions induced by early overfeeding in adult male rats. Wistar rats raised in small litters (SLs, 3 pups/dam) and normal litters (NLs, 9 pups/dam) were used in models of early overfeeding and normal feeding, respectively. During the first 12 days of lactation, animals in the SL and NL groups received scopolamine butylbromide (B), while the controls received saline (S) injections. The drug treatment decreased insulin levels in pups from both groups, and as adults, these animals showed improvements in glucose tolerance, insulin sensitivity, vagus nerve activity, fat tissue accretion, insulinemia, leptinemia, body weight gain and food intake. Low glucose and cholinergic insulinotropic effects were observed in pancreatic islets from both groups. Low protein expression was observed for the muscarinic M3 acetylcholine receptor subtype (M3mAChR), although M2mAChR subtype expression was increased in SL-B islets. In addition, beta-cell density was reduced in drug-treated rats. These results indicate that early postnatal scopolamine butylbromide treatment inhibits early overfeeding-induced metabolic dysfunctions in adult rats, which might be caused by insulin decreases during lactation, associated with reduced parasympathetic activity and expression of M3mAChR in pancreatic islets.
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Ananda
authors: A
authors: Malta
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Aline Amenencia de
authors: AA
authors: Souza
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Tatiane Aparecida
authors: TA
authors: Ribeiro
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Flávio Andrade
authors: FA
authors: Francisco
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Audrei
authors: A
authors: Pavanello
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Kelly Valério
authors: KV
authors: Prates
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Laize Peron
authors: LP
authors: Tófolo
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Rosiane Aparecida
authors: RA
authors: Miranda
authors: Health Sciences Institute, Federal University of Mato Grosso, Sinop, MT, Brazil.
authors: Júlio Cezar de
authors: JC
authors: Oliveira
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Isabela Peixoto
authors: IP
authors: Martins
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Carina
authors: C
authors: Previate
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Rodrigo Mello
authors: RM
authors: Gomes
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Claudinéia Conationi da Silva
authors: CC
authors: Franco
authors: Department of Morphophysiological Sciences, State University of Maringa, Maringá, PR, Brazil.
authors: Maria Raquel Marçal
authors: MR
authors: Natali
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Kesia
authors: K
authors: Palma-Rigo
authors: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringa, Maringá, PR, Brazil.
authors: Paulo Cezar de Freitas
authors: PC
authors: Mathias
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002086:Butylscopolammonium Bromide / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D008659:Metabolic Diseases / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D009765:Obesity / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002086:Butylscopolammonium Bromide