title: The evolutionarily conserved transcription factor Sp1 controls appendage growth through Notch signaling.
abstract: The appendages of arthropods and vertebrates are not homologous structures, although the underlying genetic mechanisms that pattern them are highly conserved. Members of the Sp family of transcription factors are expressed in the developing limbs and their function is required for limb growth in both insects and chordates. Despite the fundamental and conserved role that these transcription factors play during appendage development, their target genes and the mechanisms by which they participate in control limb growth are mostly unknown. We analyzed here the individual contributions of two Drosophila Sp members, buttonhead (btd) and Sp1, during leg development. We show that Sp1 plays a more prominent role controlling leg growth than does btd We identified a regulatory function of Sp1 in Notch signaling, and performed a genome-wide transcriptome analysis to identify other potential Sp1 target genes contributing to leg growth. Our data suggest a mechanism by which the Sp factors control appendage growth through the Notch signaling.
authors: Departamento de Biología Molecular and Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid (UAM), Nicolás Cabrera 1, Madrid 28049, Spain.
authors: Sergio
authors: S
authors: Córdoba
authors: Departamento de Biología Molecular and Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid (UAM), Nicolás Cabrera 1, Madrid 28049, Spain.
authors: David
authors: D
authors: Requena
authors: Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
authors: Aurelie
authors: A
authors: Jory
authors: Departamento de Biología Molecular and Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid (UAM), Nicolás Cabrera 1, Madrid 28049, Spain.
authors: Almudena
authors: A
authors: Saiz
authors: Departamento de Biología Molecular and Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid (UAM), Nicolás Cabrera 1, Madrid 28049, Spain cestella@cbm.csic.es.
authors: Carlos
authors: 0000-0003-1551-2327
authors: C
authors: Estella
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D019143:Evolution, Molecular; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; D016329:Sp1 Transcription Factor
keywords: Appendage; Buttonhead; Drosophila; Growth; Notch; Sp1