title: MetaMass, a tool for meta-analysis of subcellular proteomics data.
abstract: We report a tool for the analysis of subcellular proteomics data, called MetaMass, based on the use of standardized lists of subcellular markers. We analyzed data from 11 studies using MetaMass, mapping the subcellular location of 5,970 proteins. Our analysis revealed large variations in the performance of subcellular fractionation protocols as well as systematic biases in protein annotation databases. The Excel and R versions of MetaMass should enhance transparency and reproducibility in subcellular proteomics.
authors: Department of Immunology, Oslo University Hospital, Oslo, Norway.|K.G. Jebsen Center for Cancer Immunotherapy, University of Oslo, Oslo, Norway.
authors: Fridtjof
authors: 0000-0002-2445-1258
authors: F
authors: Lund-Johansen
authors: Department of Immunology, Oslo University Hospital, Oslo, Norway.|Department of Dermatology, Oslo University Hospital, Oslo, Norway.
authors: Daniel
authors: D
authors: de la Rosa Carrillo
authors: Department of Immunology, Oslo University Hospital, Oslo, Norway.|K.G. Jebsen Inflammation Research Center, University of Oslo, Oslo, Norway.
authors: Adi
authors: A
authors: Mehta
authors: Department of Immunology, Oslo University Hospital, Oslo, Norway.|K.G. Jebsen Center for Cancer Immunotherapy, University of Oslo, Oslo, Norway.
authors: Krzysztof
authors: K
authors: Sikorski
authors: Department of Immunology, Oslo University Hospital, Oslo, Norway.
authors: Marit
authors: M
authors: Inngjerdingen
authors: Childhood Leukemia Investigation Prague (CLIP), Department of Pediatric Hematology and Oncology, 2nd Faculty of Medicine, Charles University Prague and University Hospital Motol, Prague, Czech Republic.
authors: Tomas
authors: 0000-0003-4475-2872
authors: T
authors: Kalina
authors: Department of Biostatistics, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway.
authors: Kjetil
authors: K
authors: Røysland
authors: Department of Immunology, Oslo University Hospital, Oslo, Norway.
authors: Gustavo Antonio
authors: GA
authors: de Souza
authors: B Division, Los Alamos National Laboratory, Los Alamos, New Mexico, USA.
authors: Andrew R M
authors: AR
authors: Bradbury
authors: Cancer Research Centre (IBMCC, CSIC-USAL), University of Salamanca, Salamanca, Spain.|Institute of Biomedical Research of Salamanca (IBSAL), University of Salamanca, Salamanca, Spain.|NUCLEUS, University of Salamanca, Salamanca, Spain.|Department of Medicine, University of Salamanca, Salamanca, Spain.
authors: Quentin
authors: Q
authors: Lecrevisse
authors: Childhood Leukemia Investigation Prague (CLIP), Department of Pediatric Hematology and Oncology, 2nd Faculty of Medicine, Charles University Prague and University Hospital Motol, Prague, Czech Republic.
authors: Jan
authors: J
authors: Stuchly
mesh_terms: D000465:Algorithms; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000378:metabolism; D015201:Meta-Analysis as Topic; D051379:Mice; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods* / Q000706:statistics & numerical data; D013347:Subcellular Fractions / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D011506:Proteins