title: Extracellular vesicles in human follicular fluid do not promote coagulation.
abstract: Body fluids contain extracellular vesicles expressing tissue factor on their surface and serve as an additional trigger for coagulation. During the menstrual cycle ovarian tissue restoration is mandatory and it is unknown whether follicular fluid might provide procoagulant substances. Within an observational study, follicular fluid from women undergoing IVF/intracytoplasmic sperm injection (ICSI) was analysed by fluorescence-activated cell sorting (FACS), electron microscopy, resistive pulse sensing (RPS), nanoparticle-tracking analysis (NTA) and fibrin generation tests (FGT). The presence of extracellular vesicles, especially CD9-positive extracellular vesicles in follicular fluid, was proven. However, clotting tests revealed no procoagulant properties of the detected extracellular vesicles.
authors: Department of Gynaecology and Obstetrics, RWTH University of Aachen, Germany.
authors: Cordula
authors: C
authors: Franz
authors: Department of Clinical Chemistry, Academic Medical Centre Amsterdam, University of Amsterdam, Amsterdam, The Netherlands.
authors: Anita N
authors: AN
authors: Böing
authors: iLABCoMM GmbH, St. Augustin, Germany.
authors: Markus
authors: M
authors: Montag
authors: Department of Gynaecological Endocrinology and Fertility Disorders, Ruprecht-Karls University of Heidelberg, Germany.
authors: Thomas
authors: T
authors: Strowitzki
authors: Department of Obstetrics, Placenta-Lab, University Hospital Jena, Germany.
authors: Udo R
authors: UR
authors: Markert
authors: Center for Reproductive Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors: Sebastiaan
authors: S
authors: Mastenbroek
authors: Department of Clinical Chemistry, Academic Medical Centre Amsterdam, University of Amsterdam, Amsterdam, The Netherlands.
authors: Rienk
authors: R
authors: Nieuwland
authors: Department of Gynaecological Endocrinology and Fertility Disorders, Ruprecht-Karls University of Heidelberg, Germany. Electronic address: Bettina.toth@med.uni-heidelberg.de.
authors: Bettina
authors: B
authors: Toth
mesh_terms: D001777:Blood Coagulation; D000067128:Extracellular Vesicles / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D005307:Fertilization in Vitro; D005434:Flow Cytometry; D015571:Follicular Fluid / Q000166:cytology*; D006801:Humans; D046529:Microscopy, Electron, Transmission
keywords: exosomes; extracellular vesicles; follicular fluid; microparticles; ovary