title: Multifunctional System-on-Glass for Lab-on-Chip applications.
abstract: Lab-on-Chip are miniaturized systems able to perform biomolecular analysis in shorter time and with lower reagent consumption than a standard laboratory. Their miniaturization interferes with the multiple functions that the biochemical procedures require. In order to address this issue, our paper presents, for the first time, the integration on a single glass substrate of different thin film technologies in order to develop a multifunctional platform suitable for on-chip thermal treatments and on-chip detection of biomolecules. The proposed System on-Glass hosts thin metal films acting as heating sources; hydrogenated amorphous silicon diodes acting both as temperature sensors to monitor the temperature distribution and photosensors for the on-chip detection and a ground plane ensuring that the heater operation does not affect the photodiode currents. The sequence of the technological steps, the deposition temperatures of the thin films and the parameters of the photolithographic processes have been optimized in order to overcome all the issues of the technological integration. The device has been designed, fabricated and tested for the implementation of DNA amplification through the Polymerase Chain Reaction (PCR) with thermal cycling among three different temperatures on a single site. The glass has been connected to an electronic system that drives the heaters and controls the temperature and light sensors. It has been optically and thermally coupled with another glass hosting a microfluidic network made in polydimethylsiloxane that includes thermally actuated microvalves and a PCR process chamber. The successful DNA amplification has been verified off-chip by using a standard fluorometer.
authors: Department of Information Engineering, Electronics and Telecommunications, University of Rome "La Sapienza", via Eudossiana 18, Rome, 00184 Italy.
authors: G
authors: G
authors: Petrucci
authors: Department of Information Engineering, Electronics and Telecommunications, University of Rome "La Sapienza", via Eudossiana 18, Rome, 00184 Italy. Electronic address: domenico.caputo@uniroma1.it.
authors: D
authors: D
authors: Caputo
authors: Department of Information Engineering, Electronics and Telecommunications, University of Rome "La Sapienza", via Eudossiana 18, Rome, 00184 Italy.
authors: N
authors: N
authors: Lovecchio
authors: Department of Chemistry, University of Rome "La Sapienza", Piazzale Aldo Moro 5, Rome, 00185 Italy; School of Aerospace Engineering, University of Rome "La Sapienza", via Salaria 851/881, Rome, 00138 Italy.
authors: F
authors: F
authors: Costantini
authors: Department of Biology and Biotechnology "C. Darwin", University of Rome "La Sapienza", Piazzale Aldo Moro 5, Rome, 00185 Italy.
authors: I
authors: I
authors: Legnini
authors: Department of Biology and Biotechnology "C. Darwin", University of Rome "La Sapienza", Piazzale Aldo Moro 5, Rome, 00185 Italy.
authors: I
authors: I
authors: Bozzoni
authors: School of Aerospace Engineering, University of Rome "La Sapienza", via Salaria 851/881, Rome, 00138 Italy.
authors: A
authors: A
authors: Nascetti
authors: Department of Information Engineering, Electronics and Telecommunications, University of Rome "La Sapienza", via Eudossiana 18, Rome, 00184 Italy.
authors: G
authors: G
authors: de Cesare
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D016133:Polymerase Chain Reaction; D012825:Silicon / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon; D004247:DNA; D012825:Silicon
keywords: Amorphous silicon sensors; Lab-on-Chip; Microfluidics; PCR; Thin film heater