title: Lipidomic Perturbations in Lung, Kidney, and Liver Tissues of p53 Knockout Mice Analyzed by Nanoflow UPLC-ESI-MS/MS.
abstract: Lipids are important signaling molecules regulating biological processes under normal and diseased conditions. Although p53 mutation is well-known for causing cancer, the relationship between p53-related tumorigenesis and altered lipid profile is unclear. We profiled differences in lipid expressions in liver, lung, and kidney in p53 knockout (KO) mice by high-speed quantitative analysis of 320 lipids (399 species identified) using nanoflow ultrahigh performance liquid chromatography-tandem mass spectrometry (nUPLC-MS/MS). Lung tissues were most severely affected by the lack of p53 gene, as shown by significant reduction (24-44%, P < 0.05) in total phosphatidylcholine (PC), phosphatidylethanolamine (PE), sphingomyelin (SM), diacylglycerol (DG), and triacylglycerol (TG), and significant increases (30-50%) in phosphatidylserine (PS), phosphatidylinositol (PI), and monohexosylceramide (MHC). MHC levels increased in all tissues. Dihexosylceramide (DHC) level decreased only in kidney tissue. Most PI, PS, and phosphatidic acid (PA) species showing significant increases contained a saturated acyl chain (18:0) in lung and liver tissues. Neutral glycerolipids (16:0/22:0-DG and most TGs with saturated and monounsaturated acyl chains) decreased 2-4-fold in the liver tissue. Our results suggest that the lack of p53 and altered lipid profiles are closely related, but as their changes vary from one tissue to another, the lipid alterations are tissue-specific.
authors: Department of Chemistry, Yonsei University , Seoul 03722, Korea.
authors: Se Mi
authors: SM
authors: Park
authors: Department of Chemistry, Yonsei University , Seoul 03722, Korea.
authors: Seul Kee
authors: SK
authors: Byeon
authors: College of Veterinary Medicine, BK21 Program for Veterinary Science and Research Institute of Veterinary Science, Seoul National University , Seoul 08826, Korea.|Korea Mouse Phenotyping Center (KMPC) , Seoul 08826, Korea.
authors: Hyerim
authors: H
authors: Sung
authors: College of Veterinary Medicine, BK21 Program for Veterinary Science and Research Institute of Veterinary Science, Seoul National University , Seoul 08826, Korea.|Korea Mouse Phenotyping Center (KMPC) , Seoul 08826, Korea.
authors: Soo Young
authors: SY
authors: Cho
authors: College of Veterinary Medicine, BK21 Program for Veterinary Science and Research Institute of Veterinary Science, Seoul National University , Seoul 08826, Korea.|Korea Mouse Phenotyping Center (KMPC) , Seoul 08826, Korea.
authors: Je Kyung
authors: JK
authors: Seong
authors: Department of Chemistry, Yonsei University , Seoul 03722, Korea.
authors: Myeong Hee
authors: MH
authors: Moon
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D007668:Kidney / Q000737:chemistry* / Q000378:metabolism; D050356:Lipid Metabolism; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008168:Lung / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009928:Organ Specificity; D053719:Tandem Mass Spectrometry; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency* / Q000235:genetics
chemical_list: D016159:Tumor Suppressor Protein p53
keywords: kidney; lipidomic analysis; liver; lung; nUPLC-ESI-MS/MS; p53 knockout mouse