title: Serum Levels of Selected Vitamins and Trace Elements in Nigerian Consumers of Alcoholic Beverage: A Suggestion for DNA Hypomethylation.
abstract: Folic acid, vitamins and Zinc play essential role in DNA methylation but alcohol consumption is known to affectthe levels of these micronutrients leading to risk of developing various illnesses and certain cancers. This study determinedthe levels of DNA methylation dependent-micronutrients (folate, vitamin B12, vitamin B6, zinc and selenium) andhomocysteine as a suggestion for DNA methylation status in Nigerian alcohol consumers compared with non-consumers ofalcohol. Venous blood (5ml) was obtained from thirty-four males that consume alcoholic beverages for at least 10 years andthirty-two male controls that did not consume alcoholic beverages at least 10 years. Serum concentrations of folate, vitaminB12, vitamin B6, homocysteine (Hcy), selenium (Se) and zinc (Zn) were determined using High Performance LiquidChromatography (HPLC) and Atomic Absorption Spectrophotometry (AAS) as appropriate. Independent Student t-test wasused to compare the mean values between alcohol consumers and control. Mean differences were considered significant atp<0.05. The mean serum levels of Zn and Se were significantly raised in alcohol consumers when compared with nonalcohol consumers while the mean levels of Vitamin B6 and Hcy were significantly reduced in alcohol consumers whencompared with non-alcohol consumers. There were no statistically significant differences in the mean serum levels ofVitamin B12 and folate in alcohol consumers when compared with non-alcohol consumers. Since vitamin B6 and Hcy arerequired for DNA methylation, reduced vitamin B6 and Hcy levels in consumers of alcoholic beverages might suggest DNAhypomethylation in alcohol consumers.
authors: Department of Chemical Pathology, College of Medicine, University of Ibadan. drarinolaog64@yahoo.com.
authors: A N
authors: AN
authors: Ude
authors: V F
authors: VF
authors: Edem
authors: A A
authors: AA
authors: Onifade
authors: O G
authors: OG
authors: Arinola
mesh_terms: D000328:Adult; D064829:Alcohol Abstinence / Q000639:trends*; D000428:Alcohol Drinking / Q000097:blood* / Q000639:trends*; D000434:Alcoholic Beverages / Q000009:adverse effects; D015415:Biomarkers / Q000097:blood; D019175:DNA Methylation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014131:Trace Elements / Q000037:antagonists & inhibitors / Q000097:blood*
chemical_list: D015415:Biomarkers; D014131:Trace Elements