title: In vivo protective effect of phosphatidylcholine on carbon tetrachloride induced nephrotoxicity.
abstract: Phosphatidylcholine (PC) from egg yolk is a bioactive substance with various beneficial effects, including anti-inflammatory and anti-oxidant effects. Recently, this substance has been reported to prevent acute hepatotoxicity. In the present study, we aimed to evaluate the putative protective effect of PC on carbon tetrachloride (CCl4)-induced nephrotoxicity in ICR mice. Many previous studies demonstrated that CCl4induces nephrotoxicity resulting in renal oxidative damage. CCl4in corn oil (0.1ml, 1.2g/kg) was intra-peritoneally injected into 7-week-old ICR mice twice a week. PC in corn oil (0.1ml, 100mg/kg) was then orally injected daily for a week. In 7 days, blood urea nitrogen (BUN) and creatinine concentrations had significantly increased in the CCl4group compared to the control group, whereas the PC and CCl4co-injected group had significantly decreased BUN and creatinine concentrations compared to the CCl4group. Comparative analysis of histopathological injuries revealed that PC abrogated the nephrotoxicity of CCl4at 7 days. Accordingly, PC also improved renal fibrosis induced by CCl4. Various biomarkers associated with oxidative damage appeared to be up-regulated in the CCl4group, whereas in the PC and CCl4co-injected group, levels of oxidative damage significantly decreased. Aquaporin1 (AQP1), an important water transport protein in the kidney, was down regulated in the CCl4group compared to the control group. PC counteracted this effect. These results strongly suggest that PC can protect against oxidative damage induced by CCl4in the kidney and enhance recovery from renal disorders.
authors: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, 79 Gobongro, Iksan, 54596, Republic of Korea.
authors: Sokho
authors: S
authors: Kim
authors: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, 79 Gobongro, Iksan, 54596, Republic of Korea.
authors: Ji-Young
authors: JY
authors: Na
authors: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, 79 Gobongro, Iksan, 54596, Republic of Korea.
authors: Kibbeum
authors: K
authors: Song
authors: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, 79 Gobongro, Iksan, 54596, Republic of Korea. Electronic address: jkwon@jbnu.ac.kr.
authors: Jungkee
authors: J
authors: Kwon
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002252:Carbon Tetrachloride Poisoning / Q000517:prevention & control*; D004195:Disease Models, Animal; D015151:Immunoblotting; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects; D010713:Phosphatidylcholines / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000975:Antioxidants; D010713:Phosphatidylcholines
keywords: Aquaporin1; Carbon tetrachloride; Nephrotoxicity; Oxidative damage; Phosphatidylcholine