title: Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection.
abstract: Toxoplasma gondii is a ubiquitous, obligate intracellular parasite capable of crossing the placental barrier and causing spontaneous abortion, preterm labor, or significant disease in the surviving neonate. To better understand molecular mechanisms underlying abnormal pregnancy outcomes caused by T. gondii, placental proteins extracted from T. gondii-infected and -uninfected mice were comparatively analyzed using label-free liquid chromatography-tandem mass spectrometry. Significant difference was observed in the expression of 58 out of 792 proteins in infected placentas (p<0.05) compared with that in uninfected placentas. Quantitative real-time polymerase chain reaction, western blotting, and immunohistochemical staining were used to validate the results of the proteomic analysis. Some placental proteins differentially expressed in infected and uninfected mice were found to be associated with several different biological processes of pregnancy, particularly with trophoblast invasion and placental development. The results provide possible novel insights into the molecular mechanisms for abnormal pregnancy outcomes associated with T. gondii infection.
  
  
  
  In order to further explore the mechanisms of abnormal pregnant outcomes caused by T. gondii infection, we first applied label-free proteomic technology to analyze the differentially expressed host placental proteins with T. gondii infection. The results showed that some differential proteins are associated with trophoblast invasion and placenta development. The findings provide a systemic view of the altered placental proteins and help to declare the molecular mechanisms of abnormal pregnancy outcomes caused by T. gondii infection.
authors: Department of Immunology, Binzhou Medical University, Yantai, Shandong 264003, PR China.
authors: Fang
authors: F
authors: Jiao
authors: Department of Immunology, Binzhou Medical University, Yantai, Shandong 264003, PR China.
authors: Dan
authors: D
authors: Zhang
authors: Department of Gynecology and Obstetrics, Binzhou Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong 256603, PR China.
authors: Mengqi
authors: M
authors: Jiang
authors: Medicine & Pharmacy Research Center, Binzhou Medical University, Yantai, Shandong 264003, PR China.
authors: Jia
authors: J
authors: Mi
authors: Department of Immunology, Binzhou Medical University, Yantai, Shandong 264003, PR China.
authors: Xianbing
authors: X
authors: Liu
authors: Department of Immunology, Binzhou Medical University, Yantai, Shandong 264003, PR China.
authors: Haixia
authors: H
authors: Zhang
authors: Medicine & Pharmacy Research Center, Binzhou Medical University, Yantai, Shandong 264003, PR China.
authors: Zhengping
authors: Z
authors: Hu
authors: Medicine & Pharmacy Research Center, Binzhou Medical University, Yantai, Shandong 264003, PR China.
authors: Xiaoyan
authors: X
authors: Xu
authors: Department of Immunology, Binzhou Medical University, Yantai, Shandong 264003, PR China; Medicine & Pharmacy Research Center, Binzhou Medical University, Yantai, Shandong 264003, PR China. Electronic address: xue-mei-hu@163.com.
authors: Xuemei
authors: X
authors: Hu
mesh_terms: D000818:Animals; D005260:Female; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis* / Q000378:metabolism; D011257:Pregnancy Proteins / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000378:metabolism / Q000635:transmission*
chemical_list: D011257:Pregnancy Proteins
keywords: Annexins; Placenta; Protease inhibitor; Proteomic; Toxoplasma gondii