title: Vinpocetine Attenuates the Osteoblastic Differentiation of Vascular Smooth Muscle Cells.
abstract: Vascular calcification is an active process of osteoblastic differentiation of vascular smooth muscle cells; however, its definite mechanism remains unknown. Vinpocetine, a derivative of the alkaloid vincamine, has been demonstrated to inhibit the high glucose-induced proliferation of vascular smooth muscle cells; however, it remains unknown whether vinpocetine can affect the osteoblastic differentiation of vascular smooth muscle cells. We hereby investigated the effect of vinpocetine on vascular calcification using a beta-glycerophosphate-induced cell model. Our results showed that vinpocetine significantly reduced the osteoblast-like phenotypes of vascular smooth muscle cells including ALP activity, osteocalcin, collagen type I, Runx2 and BMP-2 expression as well as the formation of mineralized nodule. Vinpocetine, binding to translocation protein, induced phosphorylation of extracellular signal-related kinase and Akt and thus inhibited the translocation of nuclear factor-kappa B into the nucleus. Silencing of translocator protein significantly attenuated the inhibitory effect of vinpocetine on osteoblastic differentiation of vascular smooth muscle cells. Taken together, vinpocetine may be a promising candidate for the clinical therapy of vascular calcification.
authors: Department of Endocrinology, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, People's Republic of China.|Jining Medical University, Jining, Shandong Province, People's Republic of China.
authors: Yun-Yun
authors: YY
authors: Ma
authors: Department of Endocrinology, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, People's Republic of China.
authors: Lin
authors: L
authors: Sun
authors: Jining Medical University, Jining, Shandong Province, People's Republic of China.
authors: Xiu-Juan
authors: XJ
authors: Chen
authors: Department of Endocrinology, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, People's Republic of China.
authors: Na
authors: N
authors: Wang
authors: Department of Endocrinology, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, People's Republic of China.
authors: Peng-Fei
authors: PF
authors: Yi
authors: Department of Endocrinology, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, People's Republic of China.
authors: Min
authors: M
authors: Song
authors: Department of Endocrinology, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, People's Republic of China.
authors: Bo
authors: B
authors: Zhang
authors: Department of Neurology, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, People's Republic of China.|Central Laboratory, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, People's Republic of China.
authors: Yu-Zhong
authors: YZ
authors: Wang
authors: Department of Endocrinology, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, People's Republic of China.
authors: Qiu-Hua
authors: QH
authors: Liang
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D061205:Vascular Calcification / Q000378:metabolism*; D014748:Vinca Alkaloids / Q000494:pharmacology*
chemical_list: D010726:Phosphodiesterase Inhibitors; D014748:Vinca Alkaloids; C013983:vinpocetine; D051057:Proto-Oncogene Proteins c-akt