title: Stress-induced galectin-1 influences immune tolerance in the spleen and thymus by modulating CD45 immunoreactive lymphocytes.
abstract: Galectin-1 (Gal-1) is differentially expressed in normal and pathological tissues and regulates immune cell homeostasis. Restraint stress increases serum Gal-1 in rats. However, the function of stress-induced Gal-1 in serum is unknown. We determined if stress-induced Gal-1 in serum accumulates in immunocompetent organs as protection from physiological and/or psychological stress. Western blotting showed that the intensity of Gal-1 bands in stressed groups was significantly higher than that in controls. RT-PCR analysis indicated that the Gal-1 mRNA level did not increase after restraint stress. The numbers of Gal-1 immunoreactive cells in the splenic periarterial lymphatic sheath (PLS) and the thymus medulla of the stressed group were increased compared with those in controls. Furthermore, stress-induced Gal-1 immunoreactive cells corresponded to CD45 immunoreactive lymphocytes (CD45+) in the PLS of the spleen and the medulla of the thymus. Thus, stress-induced Gal-1 immediately accumulates in the spleen and thymus, and may modulate the immune response through apoptosis by binding to CD45+lymphocytes in immune organs following physiological and/or psychological stress.
authors: Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan. sasaguri@jichi.ac.jp.
authors: Kenichi
authors: K
authors: Sasaguri
authors: Brain Functions and Neuroscience Division, Department of Oral Science, Kanagawa Dental University Graduate School, Inaoka-cho 82, Yokosuka, Kanagawa, 238-0003, Japan.
authors: Kentaro
authors: K
authors: Yamada
authors: Division of Orthodontics, Department of Oral Science, Kanagawa Dental University Graduate School, 82 Inaoka-cho, Yokosuka, Kanagawa, 238-8580, Japan.
authors: Yuri
authors: Y
authors: Narimatsu
authors: Division of Orthodontics, Department of Oral Science, Kanagawa Dental University Graduate School, 82 Inaoka-cho, Yokosuka, Kanagawa, 238-8580, Japan.
authors: Masami
authors: M
authors: Oonuki
authors: Department of Biotechnology, Maebashi Institute of Technology, 460-1 Kamisadori-machi, Maebashi, Gunma, 371-0816, Japan.
authors: Azusa
authors: A
authors: Oishi
authors: Department of Biotechnology, Maebashi Institute of Technology, 460-1 Kamisadori-machi, Maebashi, Gunma, 371-0816, Japan.
authors: Koyo
authors: K
authors: Koda
authors: Seijoh University Graduate School of Health Care Studies, 2-172 Fukinodai, Tokai, Aichi, 476-8588, Japan.
authors: Kin-Ya
authors: KY
authors: Kubo
authors: Brain Functions and Neuroscience Division, Department of Oral Science, Kanagawa Dental University Graduate School, Inaoka-cho 82, Yokosuka, Kanagawa, 238-0003, Japan.
authors: Toshiharu
authors: T
authors: Yamamoto
authors: Department of Biotechnology, Maebashi Institute of Technology, 460-1 Kamisadori-machi, Maebashi, Gunma, 371-0816, Japan.
authors: Toshihiko
authors: T
authors: Kadoya
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D037483:Galectin 1 / Q000097:blood* / Q000235:genetics / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology / Q000378:metabolism*; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D010641:Phenotype; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D015398:Signal Transduction; D013154:Spleen / Q000276:immunology / Q000378:metabolism*; D013315:Stress, Psychological / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000276:immunology / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D037483:Galectin 1; D017493:Leukocyte Common Antigens; C516771:Ptprc protein, rat
keywords: Apoptosis; Galectin-1; Spleen; Stress; Thymus