title: JQ1, a small molecule inhibitor of BRD4, suppresses cell growth and invasion in oral squamous cell carcinoma.
abstract: The present study aimed to evaluate whether bromodomain 4 (BRD4) is expressed in Cal27 cells and to assess the effect of JQ1 on cell proliferation, apoptosis, invasion and BRD4, C-Myc and Twist expression in Cal27 cells. Immunofluorescence staining was used to determine whether BRD4 was expressed in Cal27 cells. Cell viability and proliferation were evaluated using CCK-8 assay. Flow cytometry was used to determine the apoptosis and cell cycle distribution. The cell invasion was evaluated using Transwell plate. The expression levels of BRD4, C-Myc and Twist were determined by quantitative RT-PCR (qRT-PCR) and western blotting. BRD4 was highly expressed in Cal27 cells. JQ1 inhibited cell proliferation, induced cell apoptosis, induced cell cycle arrest, and inhibited cell invasion. Gene and protein expression levels of BRD4, C-Myc and Twist were downregulated in cells treated with JQ1. JQ1 inhibited Cal27 cell growth and invasion, and downregulated expression of several oncogenes. JQ1 may be a new drug for oral squamous cell carcinoma treatment.
authors: Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong 250012, P.R. China.
authors: Limei
authors: L
authors: Wang
authors: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan, Shandong 250012, P.R. China.
authors: Xiuyin
authors: X
authors: Wu
authors: Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
authors: Ping
authors: P
authors: Huang
authors: Department of Stomatology, Laiwu City People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors: Zhijun
authors: Z
authors: Lv
authors: Department of Stomatology, Qilu Hospital, and Institute of Stomatology, Shandong University, Jinan, Shandong 250012, P.R. China.
authors: Yuping
authors: Y
authors: Qi
authors: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan, Shandong 250012, P.R. China.
authors: Xiujuan
authors: X
authors: Wei
authors: Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong 250012, P.R. China.
authors: Pishan
authors: P
authors: Yang
authors: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan, Shandong 250012, P.R. China.
authors: Fenghe
authors: F
authors: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001381:Azepines / Q000494:pharmacology*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000473:pathology; D009687:Nuclear Proteins / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D000077195:Squamous Cell Carcinoma of Head and Neck; D014157:Transcription Factors / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D014230:Triazoles / Q000494:pharmacology*; D000092468:Bromodomain Containing Proteins
chemical_list: D000970:Antineoplastic Agents; D001381:Azepines; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; D014230:Triazoles; C561695:(+)-JQ1 compound; C441289:BRD4 protein, human; D000092468:Bromodomain Containing Proteins