title: Molecularly imprinted polymer-sol-gel tablet toward micro-solid phase extraction: I. Determination of methadone in human plasma utilizing liquid chromatography-tandem mass spectrometry.
abstract: In the present work molecularly imprinted sol-gel tablet (MIP-Tablet) was prepared. The MIP-sol-gel was prepared as a thin layer on polyethylene material in a tablet form. Methadone-d9 was selected as the template and 3-(propylmethacrylate)-trimethoxysilane was used as precursor. MIP-Tablet was applied for micro-solid phase extraction (μ-SPE). The MIP-Tablet was used for the determination of methadone in human plasma samples utilizing liquid chromatography-tandem mass spectrometry; and each tablet could be used twenty times. The extraction time was 10 min while desorption time was 6 min. Factors affecting the extraction efficiency such as desorption solvents, sample pH, salt addition, extraction time, desorption time and adsorption capacity were investigated. The calibration curves were obtained within the range of 5-5000 ng/mL using methadone in human plasma samples. The coefficients of determination (r(2)) values were ≥0.999 for all runs and the extraction recovery was >80%. The accuracy values for quality control samples varied from +3.6 to +9.7% and the inter-day precision (RSD %) values were ranged from 5.0 to 8.0%. The limit of detection was 1.0 ng/mL and the lower limit of quantification was 5 ng/mL utilizing methadone in human plasma samples.
authors: Department of Environmental Sci. & Analytical Chemistry, Stockholm University, SE10691, Stockholm, Sweden.
authors: Aziza
authors: A
authors: El-Beqqali
authors: Department of Environmental Sci. & Analytical Chemistry, Stockholm University, SE10691, Stockholm, Sweden. Electronic address: mohamed.abdel-rehim@aces.su.se.
authors: Mohamed
authors: M
authors: Abdel-Rehim
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005782:Gels / Q000737:chemistry; D006801:Humans; D008691:Methadone / Q000097:blood*; D054802:Molecular Imprinting; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D052617:Solid Phase Microextraction; D013607:Tablets / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D005782:Gels; D011108:Polymers; D013607:Tablets; D008691:Methadone
keywords: Human plasma; Methadone; Micro-solid phase extraction; Molecularly imprinted polymer; Sol-gel; Tablet