title: The myosin X motor is optimized for movement on actin bundles.
abstract: Myosin X has features not found in other myosins. Its structure must underlie its unique ability to generate filopodia, which are essential for neuritogenesis, wound healing, cancer metastasis and some pathogenic infections. By determining high-resolution structures of key components of this motor, and characterizing the in vitro behaviour of the native dimer, we identify the features that explain the myosin X dimer behaviour. Single-molecule studies demonstrate that a native myosin X dimer moves on actin bundles with higher velocities and takes larger steps than on single actin filaments. The largest steps on actin bundles are larger than previously reported for artificially dimerized myosin X constructs or any other myosin. Our model and kinetic data explain why these large steps and high velocities can only occur on bundled filaments. Thus, myosin X functions as an antiparallel dimer in cells with a unique geometry optimized for movement on actin bundles.
authors: Structural Motility, Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, Sorbonne Universités, IFD, 4 Place Jussieu, 75252 Paris, France.
authors: Virginie
authors: V
authors: Ropars
authors: Department of Pharmacology and Therapeutics and the Myology Institute, University of Florida College of Medicine, PO Box 100267, Gainesville, Florida 32610-0267, USA.
authors: Zhaohui
authors: Z
authors: Yang
authors: Structural Motility, Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, Sorbonne Universités, IFD, 4 Place Jussieu, 75252 Paris, France.
authors: Tatiana
authors: T
authors: Isabet
authors: Structural Motility, Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, Sorbonne Universités, IFD, 4 Place Jussieu, 75252 Paris, France.|Laboratoire d'Ingénierie des Fonctions Moléculaires (ISIS), UMR 7006 CNRS, Université de Strasbourg, F-67083 Strasbourg Cedex, France.
authors: Florian
authors: F
authors: Blanc
authors: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA.
authors: Kaifeng
authors: K
authors: Zhou
authors: Department of Pharmacology and Therapeutics and the Myology Institute, University of Florida College of Medicine, PO Box 100267, Gainesville, Florida 32610-0267, USA.
authors: Tianming
authors: T
authors: Lin
authors: Department of Pharmacology and Therapeutics and the Myology Institute, University of Florida College of Medicine, PO Box 100267, Gainesville, Florida 32610-0267, USA.
authors: Xiaoyan
authors: X
authors: Liu
authors: Structural Motility, Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, Sorbonne Universités, IFD, 4 Place Jussieu, 75252 Paris, France.
authors: Pascale
authors: P
authors: Hissier
authors: Structural Motility, Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, Sorbonne Universités, IFD, 4 Place Jussieu, 75252 Paris, France.
authors: Frédéric
authors: F
authors: Samazan
authors: Structural Motility, Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, Sorbonne Universités, IFD, 4 Place Jussieu, 75252 Paris, France.
authors: Béatrice
authors: B
authors: Amigues
authors: Department of Pharmacology and Therapeutics and the Myology Institute, University of Florida College of Medicine, PO Box 100267, Gainesville, Florida 32610-0267, USA.
authors: Eric D
authors: ED
authors: Yang
authors: Department of Physics, Division of Life Science, and State Key Laboratory of Molecular Neuroscience. The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
authors: Hyokeun
authors: H
authors: Park
authors: Structural Motility, Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, Sorbonne Universités, IFD, 4 Place Jussieu, 75252 Paris, France.
authors: Olena
authors: O
authors: Pylypenko
authors: Laboratoire d'Ingénierie des Fonctions Moléculaires (ISIS), UMR 7006 CNRS, Université de Strasbourg, F-67083 Strasbourg Cedex, France.
authors: Marco
authors: M
authors: Cecchini
authors: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA.
authors: Charles V
authors: CV
authors: Sindelar
authors: Department of Pharmacology and Therapeutics and the Myology Institute, University of Florida College of Medicine, PO Box 100267, Gainesville, Florida 32610-0267, USA.
authors: H Lee
authors: HL
authors: Sweeney
authors: Structural Motility, Institut Curie, PSL Research University, CNRS, UMR 144, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, Sorbonne Universités, IFD, 4 Place Jussieu, 75252 Paris, France.
authors: Anne
authors: A
authors: Houdusse
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology; D018360:Crystallography, X-Ray; D019281:Dimerization; D006801:Humans; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D009218:Myosins / Q000378:metabolism* / Q000648:ultrastructure*; D011554:Pseudopodia / Q000378:metabolism*
chemical_list: C498718:MYO10 protein, human; D009218:Myosins