title: IL-7 suppresses osteogenic differentiation of periodontal ligament stem cells through inactivation of mitogen-activated protein kinase pathway.
abstract: Periodontal ligament stem cells (PDLSCs) are tissue-specific mesenchymal stem cells (MSCs), having an important role in regenerative therapy for teeth loss. Interleukin-7 (IL-7) is a key cytokine produced by stromal cells including MSCs, and exhibits specific roles for B and T cell development and osteoblasts differentiation of multiple myeloma. However, the effect of IL-7 on osteogenic differentiation of PDLSCs remains unclear. Therefore, in the present study we determined whether IL-7 affects the proliferation and osteogenic differentiation of PDLSCs in vitro and explored the associated signaling pathways for IL-7-mediated cell differentiation. The results demonstrated that the isolated human PDLSCs possessed MSCs features, highly expressing CD90, CD44, CD105, CD29 and CD73, and almost did not expressed CD34, CD45, CD11b, CD14 and CD117. IL-7 could not significantly affect the proliferation of PDLSCs, but it decreased their osteogenic differentiation and inhibited alkaline phosphatase (ALP) activity. The results of quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting exhibited that the expression levels of Runx-2, SP7 and osteocalcin (OCN) were significantly reduced by IL-7. Further studies indicated that IL-7 did not significantly change JNK, ERK1/2 and p38 protein production, but markedly suppressed their phosphorylation levels. These data suggest that IL-7 inhibits the osteogenic differentiation of PDLSCs probably via inactivation of mitogen-activated protein kinase (MAPK) signaling pathway.
authors: a Department of Stomatolog , PLA General Hospital of Chengdu Military Region, Tianhui Town , Jinniu District, Chengdu , Sichuan Province , China.
authors: Cong-Xiang
authors: CX
authors: Jian
authors: b Chengdu Military Garrison Center for Disease Control and Prevention , Chengdu , Sichuan , China.
authors: Quan-Shui
authors: QS
authors: Fan
authors: b Chengdu Military Garrison Center for Disease Control and Prevention , Chengdu , Sichuan , China.
authors: Yong-He
authors: YH
authors: Hu
authors: a Department of Stomatolog , PLA General Hospital of Chengdu Military Region, Tianhui Town , Jinniu District, Chengdu , Sichuan Province , China.
authors: Yong
authors: Y
authors: He
authors: a Department of Stomatolog , PLA General Hospital of Chengdu Military Region, Tianhui Town , Jinniu District, Chengdu , Sichuan Province , China.
authors: Ming-Zhe
authors: MZ
authors: Li
authors: a Department of Stomatolog , PLA General Hospital of Chengdu Military Region, Tianhui Town , Jinniu District, Chengdu , Sichuan Province , China.
authors: Wei-Yin
authors: WY
authors: Zheng
authors: a Department of Stomatolog , PLA General Hospital of Chengdu Military Region, Tianhui Town , Jinniu District, Chengdu , Sichuan Province , China.
authors: Yu
authors: Y
authors: Ren
authors: a Department of Stomatolog , PLA General Hospital of Chengdu Military Region, Tianhui Town , Jinniu District, Chengdu , Sichuan Province , China.
authors: Chen-Jun
authors: CJ
authors: Li
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D006801:Humans; D015851:Interleukin-7 / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D010513:Periodontal Ligament / Q000166:cytology*; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D055815:Young Adult
chemical_list: D015851:Interleukin-7; D014157:Transcription Factors
keywords: IL-7; MAPK; osteoblast; osteogenic differentiation; periodontal ligament stem cells