title: Characterization of Low-Strigolactone Germplasm in Pea (Pisum sativum L.) Resistant to Crenate Broomrape (Orobanche crenata Forsk.).
abstract: Crenate broomrape (Orobanche crenata Forsk.) is a devastating parasitic weed threatening the cultivation of legumes around the Mediterranean and in the Middle East. So far, only moderate levels of resistance were reported to occur in pea (Pisum sativum L.) natural germplasm, and most commercial cultivars are prone to severe infestation. Here, we describe the selection of a pea line highly resistant to O. crenata, following the screening of local genetic resources. Time series observations show that delayed emergence of the parasite is an important parameter associated with broomrape resistance. High performance liquid chromatography connected to tandem mass spectrometry analysis and in vitro broomrape germination bioassays suggest that the resistance mechanism might involve the reduced secretion of strigolactones, plant hormones exuded by roots and acting as signaling molecules for the germination of parasitic weeds. Two years of replicated trials in noninfested fields indicate that the resistance is devoid of pleiotropic effects on yield, in contrast to pea experimental mutants impaired in strigolactone biosynthesis and, thus, is suitable for use in breeding programs.
authors: 1 Department of Plant, Soil and Food Science, Section of Genetics and Plant Breeding, University of Bari, via Amendola 165/A, 70126 Bari, Italy.
authors: Stefano
authors: S
authors: Pavan
authors: 2 Department of Agriculture, Food and Environmental Science, University of Foggia, via Napoli 25, 71100 Foggia, Italy; and.
authors: Adalgisa
authors: A
authors: Schiavulli
authors: 1 Department of Plant, Soil and Food Science, Section of Genetics and Plant Breeding, University of Bari, via Amendola 165/A, 70126 Bari, Italy.
authors: Angelo Raffaele
authors: AR
authors: Marcotrigiano
authors: 1 Department of Plant, Soil and Food Science, Section of Genetics and Plant Breeding, University of Bari, via Amendola 165/A, 70126 Bari, Italy.
authors: Nicoletta
authors: N
authors: Bardaro
authors: 1 Department of Plant, Soil and Food Science, Section of Genetics and Plant Breeding, University of Bari, via Amendola 165/A, 70126 Bari, Italy.
authors: Valentina
authors: V
authors: Bracuto
authors: 2 Department of Agriculture, Food and Environmental Science, University of Foggia, via Napoli 25, 71100 Foggia, Italy; and.
authors: Francesca
authors: F
authors: Ricciardi
authors: 3 Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Tatsiana
authors: T
authors: Charnikhova
authors: 2 Department of Agriculture, Food and Environmental Science, University of Foggia, via Napoli 25, 71100 Foggia, Italy; and.
authors: Concetta
authors: C
authors: Lotti
authors: 3 Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors: Harro
authors: H
authors: Bouwmeester
authors: 1 Department of Plant, Soil and Food Science, Section of Genetics and Plant Breeding, University of Bari, via Amendola 165/A, 70126 Bari, Italy.
authors: Luigi
authors: L
authors: Ricciardi
mesh_terms: D001947:Breeding; D002851:Chromatography, High Pressure Liquid; D018525:Germination; D007783:Lactones / Q000378:metabolism*; D031672:Orobanche / Q000502:physiology*; D018532:Pisum sativum / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D010935:Plant Diseases / Q000276:immunology*; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D057685:Plant Weeds; D053719:Tandem Mass Spectrometry
chemical_list: D007783:Lactones; C483518:strigol