title: Target-responsive aptamer release from manganese dioxide nanosheets for electrochemical sensing of cocaine with target recycling amplification.
abstract: A novel electrochemical sensing platform based on manganese dioxide (MnO2) nanosheets was developed for sensitive screening of target cocaine with the signal amplification. Ferrocene-labeled cocaine aptamers were initially immobilized onto MnO2 nanosheets-modified screen-printed carbon electrode because of π-stacking interaction between nucleobases and nanosheets. The immobilized ferrocene-aptamer activated the electrical contact with the electrode, thereby resulting in the sensor circuit to switch on. Upon target cocaine introduction, the analyte reacted with the aptamer and caused the dissociation of ferrocene-aptamer from the electrode, thus giving rise to the detection circuit to switch off. The released aptamer was cleaved by DNase I with target recycling. Under optimal conditions, the decreasing percentage of the electronic signal relative to background current increased with the increasing cocaine concentration in the dynamic range of 0.1-20nM, and the detection limit was 32pM. The reproducibility, selectivity and method accuracy were acceptable. Importantly, this concept offers promise for rapid, simple, and cost-effective analysis of cocaine biological samples without the needs of sample separation and multiple washing steps.
authors: Key Laboratory of Applied Organic Chemistry, College of Jiangxi Province, Department of Chemistry, Shangrao Normal University, Shangrao 334001, Jiangxi, PR China.
authors: Zongbao
authors: Z
authors: Chen
authors: Institute of Environmental and Analytical Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, PR China. Electronic address: minghualu88@yeah.net.
authors: Minghua
authors: M
authors: Lu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003042:Cocaine / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D003850:Deoxyribonuclease I / Q000737:chemistry; D055664:Electrochemical Techniques; D005296:Ferrous Compounds / Q000737:chemistry; D006801:Humans; D017895:Manganese Compounds / Q000737:chemistry*; D000075163:Metallocenes / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D005296:Ferrous Compounds; D017895:Manganese Compounds; D000075163:Metallocenes; D010087:Oxides; D003850:Deoxyribonuclease I; D003042:Cocaine; C016552:manganese dioxide; C004998:ferrocene
keywords: Cocaine; Electrochemical aptasensor; Manganese dioxide nanosheets; Nuclease; Target recycling