title: Primary and tumor mouse Leydig cells exposed to polychlorinated naphthalenes mixture: Effect on estrogen related-receptors expression, intracellular calcium level and sex hormones secretion.
abstract: We report the effects of polychlorinated napthalanes (PCNs) on the mRNA expression of estrogen-related receptors (ERRs) α, β and γ, calcium (Ca2+) concentration, and sex steroid secretion in mouse primary and tumor Leydig cells. The cells were exposed to a mixture of PCNs (10nM) alone or in combination with one of sex steroid receptor antagonists; 182,780 (ICI; 10μM); hydroxyflutamide (HF; 10(-4)M) and G-coupled estrogen receptor antagonist (G15; 10nM) respectively. The expression of mRNAs and protein for ERRα, β, and γ was detected in primary and tumor Leydig cells. The expression of ERRs was always lower in primary Leydig cells. Exposure of Leydig cells to PCNs significantly increased the expression of ERRs mRNA irrespective of the cell type. Concomitantly, an increased concentration of Ca2+ and sex steroids was revealed in exposed cells. After ICI, HF or G15 was added no changes in expression of ERRs was found. In Leydig cells changes in ERRs expression at mRNA level are clearly linked to changes in Ca2+ level and steroid secretion. Estrogen and androgen receptors are not involved in PCNs action in Leydig cells. The effect of PCNs on mouse Leydig cells is independent on the cell of origin (primary or tumor).
authors: Department of Endocrinology, Institute of Zoology, Jagiellonian University in Kraków, Gronostajowa 9, 30-387 Krakow, Poland. Electronic address: laura.pardyak@doctoral.uj.edu.pl.
authors: L
authors: L
authors: Pardyak
authors: Department of Endocrinology, Institute of Zoology, Jagiellonian University in Kraków, Gronostajowa 9, 30-387 Krakow, Poland. Electronic address: alicja.kaminska@doctoral.uj.edu.pl.
authors: A
authors: A
authors: Kaminska
authors: Department of Endocrinology, Institute of Zoology, Jagiellonian University in Kraków, Gronostajowa 9, 30-387 Krakow, Poland. Electronic address: jerzy.galas@uj.edu.pl.
authors: J
authors: J
authors: Galas
authors: Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University in Kraków, Gronostajowa 9, 30-387 Krakow, Poland. Electronic address: anna.ptak@uj.edu.pl.
authors: A
authors: A
authors: Ptak
authors: Department of Endocrinology, Institute of Zoology, Jagiellonian University in Kraków, Gronostajowa 9, 30-387 Krakow, Poland. Electronic address: barbara.bilinska@uj.edu.pl.
authors: B
authors: B
authors: Bilinska
authors: Department of Endocrinology, Institute of Zoology, Jagiellonian University in Kraków, Gronostajowa 9, 30-387 Krakow, Poland. Electronic address: malgorzata.kotula-balak@uj.edu.pl.
authors: M
authors: M
authors: Kotula-Balak
mesh_terms: D000818:Animals; D052117:Benzodioxoles / Q000008:administration & dosage; D002118:Calcium / Q000378:metabolism*; D045744:Cell Line, Tumor; D047628:Estrogen Receptor alpha / Q000096:biosynthesis / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000096:biosynthesis / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism; D005485:Flutamide / Q000008:administration & dosage / Q000031:analogs & derivatives; D005786:Gene Expression Regulation / Q000187:drug effects; D012739:Gonadal Steroid Hormones / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D009281:Naphthalenes / Q000008:administration & dosage*; D011804:Quinolines / Q000008:administration & dosage; D012333:RNA, Messenger / Q000096:biosynthesis; D011944:Receptors, Androgen / Q000096:biosynthesis; D011960:Receptors, Estrogen / Q000096:biosynthesis / Q000378:metabolism
chemical_list: C568862:4-(6-bromo-1,3-benzodioxol-5-yl)-3a,4,5,9b-3H-cyclopenta(c)quinoline; D052117:Benzodioxoles; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D012739:Gonadal Steroid Hormones; D009281:Naphthalenes; D011804:Quinolines; D012333:RNA, Messenger; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; C425824:estrogen receptor gamma; C014290:hydroxyflutamide; D005485:Flutamide; D002118:Calcium
keywords: Calcium ions level; Estrogen-related receptors; Leydig cells; Polychlorinated naphthalenes; Sex steroid secretion