title: Stable isotope labeling methods for DNA.
abstract: NMR is a powerful method for studying proteins and nucleic acids in solution. The study of nucleic acids by NMR is far more challenging than for proteins, which is mainly due to the limited number of building blocks and unfavorable spectral properties. For NMR studies of DNA molecules, (site specific) isotope enrichment is required to facilitate specific NMR experiments and applications. Here, we provide a comprehensive review of isotope-labeling strategies for obtaining stable isotope labeled DNA as well as specifically stable isotope labeled building blocks required for enzymatic DNA synthesis.
authors: Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands. Electronic address: F.Nelissen@science.ru.nl.
authors: Frank H T
authors: FHT
authors: Nelissen
authors: Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands. Electronic address: M.Tessari@science.ru.nl.
authors: Marco
authors: M
authors: Tessari
authors: Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands. Electronic address: S.Wijmenga@science.ru.nl.
authors: Sybren S
authors: SS
authors: Wijmenga
authors: Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands. Electronic address: H.Heus@science.ru.nl.
authors: Hans A
authors: HA
authors: Heus
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000032:analysis* / Q000096:biosynthesis / Q000138:chemical synthesis; D007553:Isotope Labeling / Q000379:methods*; D007554:Isotopes / Q000032:analysis* / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009711:Nucleotides
chemical_list: D007554:Isotopes; D009711:Nucleotides; D004247:DNA
keywords: DNA synthesis; Labeling methods; NMR; Nucleic acids; Nucleotides; Site-specific