title: Laminin- and basement membrane-polycaprolactone blend nanofibers as a scaffold for regenerative medicine.
abstract: Mimicking one or more components of the basement membrane (BM) holds great promise for overcoming insufficiencies in tissue engineering therapies. We have electrospun laminin nanofibers (NFs) isolated from the murine Engelbreth-Holm Swarm (EHS) tumor and evaluated them as a scaffold for embryonic stem cell culture. Seeded human embryonic stem cells were found to better maintain their undifferentiated, colony environment when cultured on laminin NFs compared to laminin mats, with 75% remaining undifferentiated on NFs. Mouse embryonic stem cells cultured on 10% laminin-polycaprolactone (PCL) NFs maintained their colony formation for twice as long without passage compared to those on PCL or gelatin substrates. In addition, we have established a protocol for electrospinning reconstituted basement membrane aligned (RBM)-PCL NFs within 10° of angular deviation. Neuron-like PC12 cells show significantly greater attachment (p < 0.001) and percentage of neurite-extending cellsin vitroon 10% RBM-PCL NFs when compared to 1% and 0% RBM-PCL NFs (p < 0.015 and p < 0.001, respectively). Together, these results implicate laminin- and RBM-PCL scaffolds as a promising biomimetic substrate for regenerative medicine applications.
authors: University of Virginia Department of Biomedical Engineering, Charlottesville, VA 22903, USA.
authors: Rebekah A
authors: RA
authors: Neal
authors: University of Virginia Department of Biomedical Engineering, Charlottesville, VA 22903, USA.
authors: Steven M
authors: SM
authors: Lenz
authors: Georgia Institute of Technology and Emory University Department of Biomedical Engineering, Atlanta, Georgia 30332, USA.
authors: Tiffany
authors: T
authors: Wang
authors: University of Virginia Department of Biomedical Engineering, Charlottesville, VA 22903, USA.
authors: Daniel
authors: D
authors: Abebayehu
authors: University of Virginia Department of Biomedical Engineering, Charlottesville, VA 22903, USA.
authors: Benjamin P C
authors: BP
authors: Brooks
authors: Old Dominion University School of Medical Diagnostic & Translational Sciences, Norfolk, VA 23529, USA.
authors: Roy C
authors: RC
authors: Ogle
authors: Georgia Institute of Technology and Emory University Department of Biomedical Engineering, Atlanta, Georgia 30332, USA.
authors: Edward A
authors: EA
authors: Botchwey
keywords: basement membrane; embryonic stem cells; laminin; peripheral nerve regeneration; polycaprolactone