title: Diversity of the bacterial community in Myanmar traditional salted fish yegyo ngapi.
abstract: The distribution and characterization of bacteria including lactic acid bacteria (LAB) in the traditional and popular salted fish yegyo ngapi in Myanmar were studied to clarify the contribution of these bacteria to the curing and ripening of this product. Samples of yegyo ngapi purchased from a market in Yangon were used. Most of the isolates obtained using de Man, Rogosa and Sharpe medium containing 10 % NaCl were identified as coccoid LAB on the basis of their basic phenotypic characteristics. From the results of 16S rRNA gene sequencing and PCR-restriction fragment length polymorphism analysis of this gene, most of the isolates were identified as the halophilic LAB Tetragenococcus muriaticus. Analyses of the 16S rRNA gene based on the clone library using DNA extracted from salted fish products were also performed. The results of these molecular-analysis-based techniques showed that spore-forming and non-spore-forming anaerobic bacteria including the genera Clostridium and Halanaerobium in addition to T. muriaticus were also frequently found in bacterial communities. These findings suggest that the anaerobic condition during curing and ripening resulted in bacterial communities composed of strictly anaerobic bacteria and halophilic LAB, and that these bacteria might also contribute to the manufacturing processes of this product. In addition, DNA sequences similar to that of Clostridium botulinum were found in the clone library analysis. Therefore, despite no reports of botulism poisoning from the region where the samples were taken, closer surveillance should be carried out from the viewpoint of food safety.
authors: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan. takeshik@kaiyodai.ac.jp.
authors: Takeshi
authors: T
authors: Kobayashi
authors: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan.
authors: Chihiro
authors: C
authors: Taguchi
authors: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan.
authors: Kakeru
authors: K
authors: Kida
authors: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan.
authors: Hiroko
authors: H
authors: Matsuda
authors: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan.
authors: Takeshi
authors: T
authors: Terahara
authors: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan.
authors: Chiaki
authors: C
authors: Imada
authors: Department of Fisheries, Ministry of Agriculture, Livestock and Irrigation, Bayintnaung Road, West Gyo Gone, Insein Township, Yangon, Myanmar.
authors: Nant Kay Thwe
authors: NK
authors: Moe
authors: Department of Fisheries, Ministry of Agriculture, Livestock and Irrigation, Bayintnaung Road, West Gyo Gone, Insein Township, Yangon, Myanmar.
authors: Su Myo
authors: SM
authors: Thwe
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D044822:Biodiversity; D005285:Fermentation; D005399:Fishes / Q000382:microbiology*; D005516:Food Microbiology; D015723:Gene Library; D002052:Myanmar; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA / Q000379:methods
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Clone library; Clostridium; Lactic acid bacteria; Myanmar; Tetragenococcus; Traditional salted fish