title: Long non-coding RNA HOTAIR modulates HLA-G expression by absorbing miR-148a in human cervical cancer.
abstract: The long non-coding RNA HOX transcript antisense RNA (HOTAIR) has been found overexpressed in many human malignancies and involved in tumor progression and metastasis. However, little is known about the potential biological roles of HOTAIR in tumor escape. In the present study, the expression of HOTAIR was detected in 59 paired cervical cancer tissue samples by real-time PCR and then subjected to correlation analysis with clinical features. The effects of HOTAIR on cervical cancer cells as well as the expression of human leukocyte antigen (HLA)-G were studied by overexpression and RNA interference approaches. Insight into the mechanism of HOTAIR acting as competitive endogenous RNAs (ceRNAs) was gained from bioinformatic analysis and luciferase assays. HOTAIR expression was obviously increased in cervical cancer tissue. HOTAIR upregulation was associated with advanced pathological stage, histology, lymph node invasion and lymphatic metastasis, and also correlated with shorter overall survival of cervical cancer patients. Furthermore, HOTAIR overexpression promoted the proliferation, migration and invasion of cervical cancer cells, while HOTAIR knockdown inhibited cell invasion and cell viability, induced apoptosis and inhibited growth in vitro and in vivo. Moreover, HOTAIR modulated human leucocyte antigen-G (HLA-G) expression by competitively binding miR-148a. Our data suggest that HOTAIR plays an important oncogenic role in cervical cancer and might serve as a marker for cervical cancer prognosis and a potential target for therapeutic intervention.
authors: Department of Gynecological Ward, People's Hospital, Cangzhou, Hebei 061000, P.R. China.
authors: Jinbao
authors: J
authors: Sun
authors: Department of Obstetrics-Gynecology, Daqing LongNan Hospital, Daqing, Heilongjiang 163001, P.R. China.
authors: Haipeng
authors: H
authors: Chu
authors: Department of Gynecological Ward, People's Hospital, Cangzhou, Hebei 061000, P.R. China.
authors: Jianghai
authors: J
authors: Ji
authors: Department of Gynecological Ward, People's Hospital, Cangzhou, Hebei 061000, P.R. China.
authors: Gaoxiang
authors: G
authors: Huo
authors: Department of Gynecological Ward, People's Hospital, Cangzhou, Hebei 061000, P.R. China.
authors: Qinglei
authors: Q
authors: Song
authors: Department of Gynecological Ward, People's Hospital, Cangzhou, Hebei 061000, P.R. China.
authors: Xue
authors: X
authors: Zhang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D059951:HLA-G Antigens / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009368:Neoplasm Transplantation; D062085:RNA, Long Noncoding / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: D059951:HLA-G Antigens; C561566:HOTAIR long untranslated RNA, human; C550570:MIRN148 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding