title: The outer membrane proteins of Stenotrophomonas maltophilia are potential vaccine candidates for channel catfish (Ictalurus punctatus).
abstract: Channel catfish (Ictalurus punctatus) is an important agricultural fish that has been plagued by Stenotrophomonas maltophilia (S. maltophilia) infections in recent years, some of them severe. The outer membrane proteins (OMPs) of S. maltophilia are one of the most immunogenic and highly conserved candidates for vaccine development in aquaculture. The present study investigated OMPs of S. maltophilia as vaccine on immune response and disease resistance against S. maltophilia of channel catfish and investigated the enhancement effect of natural adjuvants Propolis (Pro), FIG polysaccharide (Fcps), Glycyrrhizine (Gly) to OMPs of S. maltophilia for further study. The results indicated that channel catfish injected intraperitoneally with OMPs showed better immune response including leukocytes phagocytosis activity, serum bactericidal activity, complement C3, IgM level and an increasement of resistance against S. maltophilia compared to the control group. Moreover, Pro, Fcps and Gly could enhance the immune response of OMPs. The relative percent of survival (RPS) was 73.33%, 66.67%, 63.33%, 60%, 0% in fish injected OMPs + Pro, OMPs + Fcps, OMPs + Gly, OMPs and 0.65% normal saline, respectively. These results suggested that OMPs used as vaccine could induce and stimulate immune response and enhance disease resistance in channel catfish, especially for Pro as immunoenhancer. Results revealed that OMPs were an effective vaccine against S. maltophilia in channel catfish.
authors: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China.
authors: Xingli
authors: X
authors: Wang
authors: Department of Aquaculture, Sichuan Province Bureau of Aquatic Products, Chenghua District Donghong Road No. 60, Chengdu 610072, China.
authors: Lang
authors: L
authors: Peng
authors: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China. Electronic address: kywang1955@126.com.
authors: Kaiyu
authors: K
authors: Wang
authors: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China.
authors: Jun
authors: J
authors: Wang
authors: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China.
authors: Yang
authors: Y
authors: He
authors: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China.
authors: Erlong
authors: E
authors: Wang
authors: Department of Aquaculture, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China.
authors: Defang
authors: D
authors: Chen
authors: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China.
authors: Ping
authors: P
authors: Ouyang
authors: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China.
authors: Yi
authors: Y
authors: Geng
authors: Department of Aquaculture, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China.
authors: Xiaoli
authors: X
authors: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001428:Bacterial Vaccines / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D007059:Ictaluridae; D016415:Sequence Alignment / Q000662:veterinary; D020615:Stenotrophomonas maltophilia / Q000276:immunology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines
keywords: Channel catfish; Immune effect; Outer membrane proteins; Stenotrophomonas maltophilia