title: Immunochromatographic strip assay for detection of bioactive Ganoderma triterpenoid, ganoderic acid A in Ganoderma lingzhi.
abstract: Ganoderic acid A (GAA) is one of the major Ganoderma triterpenes produced by medicinal mushroom belonging to the genus Ganoderma (Ganodermataceae). Due to its interesting pharmacological activities, Ganoderma species have been traditionally used in China for the treatment of various diseases. Herein, we developed a colloidal gold-based immunochromatographic strip assay (ICA) for the rapid detection of GAA using highly specific monoclonal antibody against GAA (MAb 12A) conjugated with gold nanoparticles. Using the developed ICA, the detection of GAA can be completed within 15min after dipping the test strip into an analyte solution with the limit of detection (LOD) for GAA of ~500ng/mL. In addition, this system makes it possible to perform a semi-quantitative analysis of GAA in Ganoderma lingzhi, where high reliability was evaluated by enzyme-linked immunosorbent assay (ELISA). The newly developed ICA can potentially be applied to the standardization of Ganoderma using GAA as an index because GAA is major triterpenoid present much in the mushroom.
authors: Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors: Seiichi
authors: S
authors: Sakamoto
authors: Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors: Nao
authors: N
authors: Kikkawa
authors: Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors: Toshitaka
authors: T
authors: Kohno
authors: Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; Department of Agro-Environmental Sciences, Graduate School of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors: Kuniyoshi
authors: K
authors: Shimizu
authors: Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: htanaka@phar.kyushu-u.ac.jp.
authors: Hiroyuki
authors: H
authors: Tanaka
authors: Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors: Satoshi
authors: S
authors: Morimoto
mesh_terms: D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D032902:Ganoderma / Q000737:chemistry*; D006538:Heptanoic Acids / Q000302:isolation & purification*; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000302:isolation & purification; D057230:Limit of Detection; D015203:Reproducibility of Results
chemical_list: D000911:Antibodies, Monoclonal; D006538:Heptanoic Acids; D007810:Lanosterol; C515005:ganoderic acid A
keywords: Colloidal gold; Ganoderic acid A; Ganoderma lingzhi; Immunochromatographic assay; Monoclonal antibody