title: Circulating Cd34+ cell count differentiates primary myelofibrosis from other Philadelphia-negative myeloproliferative neoplasms: a pragmatic study.
abstract: A high number of circulating CD34+ cells has been advocated to distinguish primary myelofibrosis from other Philadelphia-negative myeloproliferative neoplasms. We re-evaluated the diagnostic interest of measuring circulating CD34+ cells in 26 healthy volunteers and 256 consecutive patients at diagnosis for whom a myeloproliferative neoplasm was suspected. The ROC curve analysis showed that a number of CD34+ <10/μl excludes the diagnosis of primary myelofibrosis with a sensitivity of 97 % and a specificity of 90 % (area under the curve: 0.93 [0.89-0.98]; p < 0.001). Patients with PMF harboring a CALR mutation had more circulating CD34+ cells than patients with either a JAK 2 or MPL mutation (p = 0.02 and p < 0.01, respectively). These results suggest that this fast, simple, non-invasive, and standardized test is of particular interest to exclude the diagnosis of primary myelofibrosis.
authors: Service des Maladies du Sang, CHU d'Angers, Angers, France.|Laboratoire d'Hématologie, CHU d'Angers, Angers, France.|INSERM U892/CNRS 6295, Université d'Angers, Angers, France.
authors: C
authors: C
authors: Orvain
authors: Laboratoire d'Hématologie, CHU d'Angers, Angers, France.|Laboratoire d'Hématologie, CHU de Brest, Brest, France.|Université d'Angers, UFR Santé, Angers, France.
authors: D
authors: D
authors: Luque Paz
authors: Laboratoire d'Hématologie, CHU d'Angers, Angers, France.
authors: I
authors: I
authors: Dobo
authors: Laboratoire d'Hématologie, CHU d'Angers, Angers, France.
authors: L
authors: L
authors: Cottin
authors: Laboratoire d'Hématologie, CHU de Brest, Brest, France.
authors: G
authors: G
authors: Le Calvez
authors: Laboratoire d'Hématologie, CHU de Brest, Brest, France.
authors: A
authors: A
authors: Chauveau
authors: Service des Maladies du Sang, CHU d'Angers, Angers, France.
authors: M
authors: M
authors: Mercier
authors: Service des Maladies du Sang, CHU d'Angers, Angers, France.
authors: J
authors: J
authors: Farhi
authors: Service des Maladies du Sang, CHU d'Angers, Angers, France.
authors: F
authors: F
authors: Boyer
authors: Service d'Hématologie Clinique, Institut de Cancéro-Hématologie, CHU de Brest, Brest, France.
authors: J C
authors: JC
authors: Ianotto
authors: Service d'Anatomie et Cytologie Pathologiques, CHU de Brest, Brest, France.
authors: B
authors: B
authors: Guibourg
authors: Département de Pathologie Cellulaire et Tissulaire, CHU d'Angers, Angers, France.
authors: M C
authors: MC
authors: Rousselet
authors: Laboratoire d'Hématologie, CHU d'Angers, Angers, France.
authors: M
authors: M
authors: Zandecki
authors: Service des Maladies du Sang, CHU d'Angers, Angers, France.|INSERM U892/CNRS 6295, Université d'Angers, Angers, France.|Université d'Angers, UFR Santé, Angers, France.
authors: N
authors: N
authors: Ifrah
authors: Service des Maladies du Sang, CHU d'Angers, Angers, France.|INSERM U892/CNRS 6295, Université d'Angers, Angers, France.|Université d'Angers, UFR Santé, Angers, France.
authors: M
authors: M
authors: Hunault-Berger
authors: Laboratoire d'Hématologie, CHU d'Angers, Angers, France.|INSERM U892/CNRS 6295, Université d'Angers, Angers, France.|Laboratoire d'Hématologie, CHU de Brest, Brest, France.|Université d'Angers, UFR Santé, Angers, France.
authors: V
authors: V
authors: Ugo
authors: Laboratoire d'Hématologie, CHU d'Angers, Angers, France. frgenevieve@chu-angers.fr.
authors: F
authors: F
authors: Genevieve
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis; D019540:Area Under Curve; D001772:Blood Cell Count; D037282:Calreticulin / Q000235:genetics; D004252:DNA Mutational Analysis; D006412:Hematopoietic Stem Cells; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics; D009154:Mutation; D009190:Myelodysplastic Syndromes / Q000097:blood / Q000175:diagnosis; D009196:Myeloproliferative Disorders / Q000097:blood / Q000175:diagnosis; D055728:Primary Myelofibrosis / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D012372:ROC Curve; D053628:Receptors, Thrombopoietin / Q000235:genetics; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
chemical_list: D018952:Antigens, CD34; C576483:CALR protein, human; D037282:Calreticulin; D053628:Receptors, Thrombopoietin; C082218:MPL protein, human; C507924:JAK2 protein, human; D053614:Janus Kinase 2
keywords: Circulating CD34+ cells; Myeloproliferative neoplasms (MPN); Primary myelofibrosis (PMF)