title: Preparative isolation of cordycepin, N(6)-(2-hydroxyethyl)-adenosine and adenosine from Cordyceps militaris by macroporous resin and purification by recycling high-speed counter-current chromatography.
abstract: In this study, cordycepin, N(6)-(2-hydroxyethyl)-adenosine (HEA) and adenosine from the fruiting bodies of Cordyceps militaris were separated by using macroporous resin NKA-II adsorption. The parameters of static adsorption were tested and the optimized conditions were as follow: the total adsorption time was 12h, 50% ethanol was used for desorption and the desorption time was 9h. The crude sample that was prepared by macroporous resin NKA-II contained 3.4% cordycepin, 3.7% HEA and 4.9% adenosine. Then the crude sample was further purified by recycling high-speed counter-current chromatography (HSCCC) with ethyl acetate, n-butanol, 1.5% aqueous ammonium hydroxide (1:4:5, v/v/v) as the optimized two-phase solvent system. Three nucleosides including 15.6mg of cordycepin, 16.9mg of HEA and 23.2mg of adenosine were obtained from 500mg of crude sample in one-step separation. The purities of three compounds were 98.5, 98.3 and 98.0%, respectively, as determined by high performance liquid chromatography.
authors: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.
authors: Zhong
authors: Z
authors: Zhang
authors: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China; College of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
authors: Tuernisan
authors: T
authors: Tudi
authors: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.
authors: Yanfang
authors: Y
authors: Liu
authors: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.
authors: Shuai
authors: S
authors: Zhou
authors: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.
authors: Na
authors: N
authors: Feng
authors: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.
authors: Yan
authors: Y
authors: Yang
authors: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.
authors: Chuanhong
authors: C
authors: Tang
authors: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China; College of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China. Electronic address: tangqingjiu@saas.sh.cn.
authors: Qingjiu
authors: Q
authors: Tang
authors: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China. Electronic address: syja16@saas.sh.cn.
authors: Jingsong
authors: J
authors: Zhang
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*; D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D032661:Cordyceps / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000379:methods*; D003839:Deoxyadenosines / Q000737:chemistry / Q000302:isolation & purification*; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*; D012997:Solvents
chemical_list: D003839:Deoxyadenosines; C528186:N6-(2-hydroxyethyl)adenosine; D012117:Resins, Synthetic; D012997:Solvents; C058120:cordycepin; D000241:Adenosine
keywords: Adenosine; Cordycepin; Cordyceps militaris; High-speed counter-current chromatography; Macroporous resin; N(6)-(2-hydroxyethyl)-adenosine