title: Urocortin and corticotrophin-releasing hormone receptor type 2 mRNA are highly expressed in deep infiltrating endometriotic lesions.
abstract: Ovarian endometrioma (OMA) and deep infiltrating endometriosis (DIE) are the most severe forms of endometriosis, but different pathogenetic mechanisms and clinical symptoms distinguish these two forms. Corticotrophin-releasing hormone (CRH) and urocortin (Ucn) are endometrial neuropeptides involved in tissue differentiation and inflammation. The expression of CRH, Ucn, Ucn2, CRH-receptors (type-1 and type-2) and inflammatory enzymes phospholipase-A2 group IIA (PLA2G2A) and cycloxygenase-2 (COX2) were evaluated in OMA (n = 22) and DIE (n = 26). The effect of CRH or Ucn on COX2 mRNA expression was evaluated in cultured human endometrial stromal cells. In DIE lesions, CRH, Ucn and CRH-R2 mRNA levels were significantly higher than in OMA (P < 0.01, P < 0.001 and P < 0.05, respectively); DIE lesions showed a higher expression of COX2 (P < 0.01) and PLA2G2A (P < 0.05) mRNA than OMA, which was positively correlated with CRH-R2 mRNA expression (P < 0.05). Intense immunostaining for CRH and Ucn was shown in DIE. Treatment of cultured endometrial stromal cells with Ucn significantly increased COX2 mRNA expression (P < 0.01); this effect was reversed by the CRH-R2 antagonist astressin-2B. In DIE, DIE lesions highly express neuropeptide and enzyme mRNAs, supporting a strong activation of inflammatory pathways.
authors: Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy.
authors: Patrizia
authors: P
authors: Carrarelli
authors: Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy.
authors: Alice
authors: A
authors: Luddi
authors: Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy.
authors: Lucia
authors: L
authors: Funghi
authors: Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy.
authors: Felice
authors: F
authors: Arcuri
authors: Department of Immunology, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris (AP-HP), 75014 Paris, France; Sorbonne Paris Cité, Inserm, Unité de Recherche U1016, Institut Cochin, CNRS (UMR 8104), Université Paris Descartes, Paris, France.
authors: Frederic
authors: F
authors: Batteux
authors: Department of Obstetrics and Gynecology, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors: Cynthia
authors: C
authors: Dela Cruz
authors: Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy.
authors: Claudia
authors: C
authors: Tosti
authors: Department of Obstetrics and Gynecology, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors: Fernando M
authors: FM
authors: Reis
authors: Sorbonne Paris Cité, Inserm, Unité de Recherche U1016, Institut Cochin, CNRS (UMR 8104), Université Paris Descartes, Paris, France; Sorbone Paris Cité, Faculté de Médecine, Assistance Publique - Hôpitaux de Paris (AP-HP), Groupe Hospitalier Universitaire (GHU) Ouest, Centre Hospitalier Universitaire (CHU) Cochin, Department of Gynecology Obstetrics II and Reproductive Medicine (Profesor Chapron), Université Paris Descartes, Paris France.
authors: Charles
authors: C
authors: Chapron
authors: Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy. Electronic address: felice.petraglia@unisi.it.
authors: Felice
authors: F
authors: Petraglia
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005260:Female; D054501:Group II Phospholipases A2 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D010049:Ovarian Diseases / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010446:Peptide Fragments / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism; D054832:Urocortins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D055815:Young Adult
chemical_list: C101531:CRF receptor type 2; D010446:Peptide Fragments; D010456:Peptides, Cyclic; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D054832:Urocortins; C528574:astressin-2B; C104416:astressin; D003346:Corticotropin-Releasing Hormone; D051546:Cyclooxygenase 2; D054501:Group II Phospholipases A2; C516287:PLA2G2A protein, human
keywords: COX2; CRH/CRH-R2; PLA2G2A; deep infiltrating endometriosis; endometrioma; urocortin