title: Development of a Multiplex PCR for Discrimination of the TLC:RS1:CTX array ofVibrio choleraeWave 3 El Tor Strains.
abstract: Vibrio choleraeO1 serogroup Wave 3 El Tor strains are presently prevalent worldwide. The Wave 3 El Tor strains contain a TLC:RS1:CTX array on chromosome 1, and no element is integrated on chromosome 2. A multiplex PCR optimized to identify the TLC:RS1:CTX array of Wave 3 strains has been developed in this study. By using eight primers, the multiplex PCR can identify the characteristic CTX and RS1 array of Wave 3 strains from various arrays of strains belonging to other Waves. The four amplified DNA fragments of Wave 3 strains have been cloned in a vector, which could be used as a positive control for the multiplex PCR. This multiplex PCR and the positive control set could be useful tools for rapid recognition of Wave 3 El Tor strains.
authors: Department of Pharmacy, College of Pharmacy, Hanyang University, Ansan 15588, Republic of Korea.|Institute of Pharmacological Research, Hanyang University, Ansan 15588, Republic of Korea.
authors: Eun Jin
authors: EJ
authors: Kim
authors: Department of Pharmacy, College of Pharmacy, Hanyang University, Ansan 15588, Republic of Korea.|Institute of Pharmacological Research, Hanyang University, Ansan 15588, Republic of Korea.
authors: Hyun Jin
authors: HJ
authors: Yu
authors: South East Asia Regional Office, World Health Organization, New Delhi - 110002, India.
authors: G Balakrish
authors: GB
authors: Nair
authors: Department of Pharmacy, College of Pharmacy, Hanyang University, Ansan 15588, Republic of Korea.|Institute of Pharmacological Research, Hanyang University, Ansan 15588, Republic of Korea.
authors: Dong Wook
authors: DW
authors: Kim
mesh_terms: D002771:Cholera / Q000382:microbiology*; D002772:Cholera Toxin / Q000235:genetics* / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D002772:Cholera Toxin
keywords: V. cholerae; multiplex PCR