title: Proteomic analysis reveals aberrant expression of CALR and HSPA5 in thyroid tissues of Graves' disease.
abstract: OBJECTIVE
  To explore the proteomic changes in thyroid tissue from GD patients and find new biomarkers for the prevention, diagnosis as well as the treatment of GD.
  
  
  METHODS
  Group1 included five thyroid specimens of GD cases and 5 normal thyroid tissue samples which were removed surgically and collected. The proteins were extracted from these thyroid tissues and then the differentially expressed protein spots were identified by MALDI-TOF-MS. The interested proteins were further validated in more specimens (group2: 11 pathological thyroid specimens and 7 normal thyroid tissue samples).
  
  
  RESULTS
  A total of 34 differentially expressed proteins were observed, and the majority of these proteins were involved in endoplasmic reticulum stress (ER-stress), oxidative stress, energy metabolism, cytoskeleton and movement. The overexpression of calreticulin(CALR) and heat shock 70kDa protein 5(HSPA5) was further validated.
  
  
  CONCLUSIONS
  Alltogether, abundant new candidate molecules, especially proteins related to ER-stress, were found to be involved in the pathogenesis of GD.
authors: Endocrinology Department, Jinshan Hospital of Fudan University, Shanghai 201508, China.
authors: Shuai
authors: S
authors: Meng
authors: Endocrinology Department, Jinshan Hospital of Fudan University, Shanghai 201508, China.
authors: Wen
authors: W
authors: Zhang
authors: Endocrinology Department, Jinshan Hospital of Fudan University, Shanghai 201508, China.
authors: Li-Juan
authors: LJ
authors: Guan
authors: Endocrinology Department, Jinshan Hospital of Fudan University, Shanghai 201508, China.
authors: Fatuma-Said
authors: FS
authors: Muhali
authors: Endocrinology Department, Jinshan Hospital of Fudan University, Shanghai 201508, China.
authors: Jiao-Zhen
authors: JZ
authors: Zhou
authors: Endocrinology Department, Jinshan Hospital of Fudan University, Shanghai 201508, China.
authors: Rong-Hua
authors: RH
authors: Song
authors: Endocrinology Department, Jinshan Hospital of Fudan University, Shanghai 201508, China.
authors: Jian
authors: J
authors: Xu
authors: Endocrinology Department, Jinshan Hospital of Fudan University, Shanghai 201508, China. Electronic address: zhangjinan@hotmail.com.
authors: Jin-An
authors: JA
authors: Zhang
mesh_terms: D000328:Adult; D037282:Calreticulin / Q000378:metabolism*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D006111:Graves Disease / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013961:Thyroid Gland / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
chemical_list: D037282:Calreticulin; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins
keywords: Calreticulin(CALR); Endoplasmic reticulum (ER) stress; Graves' disease(GD); Heat shock 70kDa protein 5(HSPA5); Proteomics