title: Expression of a novel endothelial marker, C-type lectin 14A, in epithelial ovarian cancer and its prognostic significance.
abstract: OBJECTIVE
  The purpose of this study was to evaluate microvessel density (MVD) as assessed by C-type lectin 14A (CLEC14A), which is a new marker for endothelial cells, and compare its expression to CD31 and CD105 in epithelial ovarian cancer (EOC).
  
  
  METHODS
  MVD was evaluated in tumors (n = 50) from patients with EOC who underwent primary surgery and in patients with EOC who received preoperative chemotherapy (n = 49) using immunohistochemistry with antibodies to CLEC14A, CD31 and CD105. The median duration of follow-up was 24.5 months (range 1-101 months). The effect of prognostic factors on event-free survival (EFS) and overall survival (OS) was assessed using the Cox regression model.
  
  
  RESULTS
  The amount of residual disease was found to be an independent prognostic factor in multivariate analysis with respect to EFS (P = 0.009) and OS (P < 0.001). The mean MVD of CLEC14A (MVD = 6), in tumors from patients who underwent primary surgery, was significantly lower than that of CD31 (MVD = 25, P < 0.0001) and CD105 (MVD = 11, P = 0.018). However, there was no significant correlation between MVD as detected by these markers and clinical outcome. There was no expression of CLEC14A in tumors from patients who received preoperative chemotherapy and the MVD of CD31 and CD105 was significantly reduced (P = 0.001 and 0.006, respectively) in this set of patients.
  
  
  CONCLUSIONS
  This study demonstrates MVD as detected by CLEC14A in EOC. Treatment with chemotherapy reduces tumor blood vessels significantly. We suggest that CLEC14A may be a more specific endothelial marker to assess tumor angiogenesis.
authors: Laboratory for Cancer Biology, Medical Oncology and Clinical Research, Cancer Institute (WIA), 38, Sardar Patel Road, Guindy, Chennai, Tamil Nadu, 600036, India.
authors: S
authors: S
authors: Krishna Priya
authors: Medical Oncology, Command Hospital (CC), Lucknow Cantt., Lucknow, UP, 226002, India.
authors: Kishore
authors: K
authors: Kumar
authors: Department of Histopathology, Amrita Institute of Medical Sciences and Research Centre, Cochin, Kerala, 682041, India.
authors: K R
authors: KR
authors: Hiran
authors: Department of Histopathology, Amrita Institute of Medical Sciences and Research Centre, Cochin, Kerala, 682041, India.
authors: M R
authors: MR
authors: Bindhu
authors: Laboratory for Cancer Biology, Medical Oncology and Clinical Research, Cancer Institute (WIA), 38, Sardar Patel Road, Guindy, Chennai, Tamil Nadu, 600036, India.
authors: Rohit P
authors: RP
authors: Nagare
authors: Department of Surgical Oncology, Amrita Institute of Medical Sciences and Research Centre, Cochin, Kerala, 682041, India.
authors: D K
authors: DK
authors: Vijaykumar
authors: Laboratory for Cancer Biology, Medical Oncology and Clinical Research, Cancer Institute (WIA), 38, Sardar Patel Road, Guindy, Chennai, Tamil Nadu, 600036, India. tsganesan@gmail.com.
authors: T S
authors: http://orcid.org/0000-0002-4671-8099
authors: TS
authors: Ganesan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D000077216:Carcinoma, Ovarian Epithelial; D015815:Cell Adhesion Molecules / Q000032:analysis*; D017024:Chemotherapy, Adjuvant; D018572:Disease-Free Survival; D000071063:Endoglin / Q000032:analysis; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000032:analysis*; D055806:Microvessels / Q000473:pathology*; D008875:Middle Aged; D020360:Neoadjuvant Therapy; D018365:Neoplasm, Residual; D009375:Neoplasms, Glandular and Epithelial / Q000098:blood supply* / Q000737:chemistry* / Q000628:therapy; D009389:Neovascularization, Pathologic; D010051:Ovarian Neoplasms / Q000098:blood supply* / Q000737:chemistry* / Q000628:therapy; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D015996:Survival Rate; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C570898:CLEC14A protein, human; D015815:Cell Adhesion Molecules; C064206:ENG protein, human; D000071063:Endoglin; D037181:Lectins, C-Type; D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: C-type lectin 14A (CLEC14A); Endothelial cells; Epithelial ovarian cancer; Microvessel density