title: Splice variants of the SWR1-type nucleosome remodeling factor Domino have distinct functions during Drosophila melanogaster oogenesis.
abstract: SWR1-type nucleosome remodeling factors replace histone H2A by variants to endow chromatin locally with specialized functionality. In Drosophila melanogaster a single H2A variant, H2A.V, combines functions of mammalian H2A.Z and H2A.X in transcription regulation and the DNA damage response. A major role in H2A.V incorporation for the only SWR1-like enzyme in flies, Domino, is assumed but not well documented in vivo. It is also unclear whether the two alternatively spliced isoforms, DOM-A and DOM-B, have redundant or specialized functions. Loss of both DOM isoforms compromises oogenesis, causing female sterility. We systematically explored roles of the two DOM isoforms during oogenesis using a cell type-specific knockdown approach. Despite their ubiquitous expression, DOM-A and DOM-B have non-redundant functions in germline and soma for egg formation. We show that chromatin incorporation of H2A.V in germline and somatic cells depends on DOM-B, whereas global incorporation in endoreplicating germline nurse cells appears to be independent of DOM. By contrast, DOM-A promotes the removal of H2A.V from stage 5 nurse cells. Remarkably, therefore, the two DOM isoforms have distinct functions in cell type-specific development and H2A.V exchange.
authors: Biomedical Center and Center for Integrated Protein Science Munich, Ludwig-Maximilians-University, Großhaderner Strasse 9, 82152 Munich, Germany.
authors: Kenneth
authors: K
authors: Börner
authors: Biomedical Center and Center for Integrated Protein Science Munich, Ludwig-Maximilians-University, Großhaderner Strasse 9, 82152 Munich, Germany pbecker@med.uni-muenchen.de.
authors: Peter B
authors: 0000-0001-7186-0372
authors: PB
authors: Becker
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism* / Q000502:physiology*; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism; D009866:Oogenesis / Q000235:genetics / Q000502:physiology*; D012326:RNA Splicing / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D014157:Transcription Factors; C499600:dom protein, Drosophila; D051548:Histone Acetyltransferases; C511056:Tip60 protein, Drosophila
keywords: Chromatin; Germline; H2A.X; H2A.Z; HIS2AV; Histone variants; Remodeling