title: The impact of allylamine-bile acid combinations on cell delivery microcapsules in diabetes.
abstract: OBJECTIVE
  In a recent study, we developed a new microencapsulating method for β-cell microencapsulation, but cell viability declined rapidly, post microencapsulation, due to potential polymer-polyelectrolyte chelation and non-porous microcapsules' membranes resulting in cell apoptosis. Thus, this study tested the effects of incorporating cationic polyamine at 1% w/v, on microcapsule strength and cell viability, in the absence or presence of an anionic tertiary bile acid (ATBA) with potential cell-protective effects.
  
  
  METHODS
  1% w/v polyamine was used without or with ATBA, to form β-cell microcapsules and physical and biological analyses was carried out 50 h post microencapsulation.
  
  
  RESULTS
  Microcapsules containing 1% w/v polyamine showed weak physical properties and low cell viability and ATBA incorporation resulted in >30% reduction in cell viability and increased levels of pro-inflammatory cytokines.
  
  
  CONCLUSIONS
  Neither 1% w/v polyamine nor the presence of ATBA resulted in optimised cell viability, but rather reduced cell viability, enhanced inflammation and lowered insulin secretion.
authors: a Biotechnology and Drug Development Research Laboratory, School of Pharmacy , Curtin Health Innovation Research Institute, Curtin University , Perth , Western Australia , Australia.
authors: Armin
authors: A
authors: Mooranian
authors: a Biotechnology and Drug Development Research Laboratory, School of Pharmacy , Curtin Health Innovation Research Institute, Curtin University , Perth , Western Australia , Australia.
authors: Rebecca
authors: R
authors: Negrulj
authors: a Biotechnology and Drug Development Research Laboratory, School of Pharmacy , Curtin Health Innovation Research Institute, Curtin University , Perth , Western Australia , Australia.
authors: Hani
authors: H
authors: Al-Salami
mesh_terms: D000499:Allylamine / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D001647:Bile Acids and Salts / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D002214:Capsules; D002460:Cell Line; D003920:Diabetes Mellitus / Q000188:drug therapy*; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D050417:Insulin-Secreting Cells / Q000378:metabolism*
chemical_list: D001647:Bile Acids and Salts; D002214:Capsules; D000499:Allylamine
keywords: Bile-acid based microcapsules; biomaterials; inflammation; insulin; β-cells