title: Amperometric biosensor based on a single antibody of dual function for rapid detection of Streptococcus agalactiae.
abstract: Pathogenic bacteria are responsible for several diseases in humans and in a variety of hosts. Detection of pathogenic bacteria is imperative to avoid and/or fight their potential harmful effects. This work reports on the first amperometric biosensor for the rapid detection of Streptococcus agalactiae (S. agalactiae). The biosensor relies on a single biotinylated antibody that immobilizes the bacteria on a screen-printed carbon electrode while is further linked to a streptavidin-conjugated HRP reporter. The biotinylated antibody provides selectivity to the biosensor whereas serves as an anchoring point to the reporter for further amplification of the electrochemical signal. The resultant immunosensor is simple, responds rapidly, and allows for the selective and highly sensitive quantification of S. agalactiae cells in a concentration range of 101-107CFUml-1, with a detection limit of 10CFUml-1. The approach not only enables a rapid detection and quantification of S. agalactiae in environmental samples but also opens up new opportunities for the simple fabrication of electrochemical immunosensors for different target pathogens.
authors: Grupo de Patología Veterinaria, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Bogotá 111321, Colombia.
authors: Gersson
authors: G
authors: Vásquez
authors: Grupo de Patología Veterinaria, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Bogotá 111321, Colombia.
authors: Alba
authors: A
authors: Rey
authors: Grupo de Patología Veterinaria, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Bogotá 111321, Colombia.
authors: Camilo
authors: C
authors: Rivera
authors: Grupo de Patología Veterinaria, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Bogotá 111321, Colombia.
authors: Carlos
authors: C
authors: Iregui
authors: Max Planck Tandem Group in Nanobioengineering, Universidad de Antioquia, Complejo Ruta N, Calle 67 No. 52-20, Medellín 050010, Colombia. Electronic address: grupotandem@udea.edu.co.
authors: Jahir
authors: J
authors: Orozco
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D055664:Electrochemical Techniques / Q000191:economics / Q000379:methods; D004566:Electrodes; D005393:Fish Diseases / Q000175:diagnosis* / Q000382:microbiology; D007118:Immunoassay / Q000191:economics / Q000379:methods; D057230:Limit of Detection; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013292:Streptococcus agalactiae / Q000302:isolation & purification*; D017210:Tilapia / Q000382:microbiology*; D014871:Water Microbiology
chemical_list: D055910:Antibodies, Immobilized
keywords: Amperometric detection; Biotinylated antibody; Immunosensor; S. agalactiae; Screen-printed carbon electrode