title: Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations.
abstract: The formation of spherical aggregates during the growth of cell population has long been observed under various conditions. We observed the formation of such aggregates during proliferation of Huh-7.5 cells, a human hepatocarcinoma cell line, in a microfabricated low-adhesion microwell system (SpheroFilm; formed of mass-producible silicone elastomer) on the length scales up to 500 μm. The cell proliferation was also tracked with immunofluorescence staining of F-actin and cell proliferation marker Ki-67. Meanwhile, our complementary 3D Monte Carlo simulations, taking cell diffusion and division, cell-cell and cell-scaffold adhesion, and gravity into account, illustrate the role of these factors in the formation of spheroids. Taken together, our experimental and simulation results provide an integrative view of the process of spheroid formation for Huh-7.5 cells.
authors: School of Materials Science and Engineering, Nanyang Technological University, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University, Singapore.
authors: Yan
authors: Y
authors: Wang
authors: School of Materials Science and Engineering, Nanyang Technological University, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University, Singapore.
authors: Myung Hee
authors: MH
authors: Kim
authors: School of Materials Science and Engineering, Nanyang Technological University, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University, Singapore.
authors: Seyed R
authors: SR
authors: Tabaei
authors: School of Materials Science and Engineering, Nanyang Technological University, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University, Singapore.
authors: Jae Hyeok
authors: JH
authors: Park
authors: School of Mechanical Engineering, Korea University, Seoul, Korea.
authors: Kyuhwan
authors: K
authors: Na
authors: School of Mechanical Engineering, Korea University, Seoul, Korea.
authors: Seok
authors: S
authors: Chung
authors: Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk, Russia.
authors: Vladimir P
authors: VP
authors: Zhdanov
authors: School of Materials Science and Engineering, Nanyang Technological University, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University, Singapore.|School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
authors: Nam-Joon
authors: http://orcid.org/0000-0002-8692-8955
authors: NJ
authors: Cho
mesh_terms: D000199:Actins / Q000378:metabolism; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D007700:Kinetics; D009010:Monte Carlo Method; D018874:Spheroids, Cellular / Q000378:metabolism / Q000473:pathology*
chemical_list: D000199:Actins; D019394:Ki-67 Antigen