title: A set of Triticum aestivum-Aegilops speltoides Robertsonian translocation lines.
abstract: Here we report the production of a set of wheat - Aegilops speltoides Robertsonian translocations covering all Ae. speltoides chromosome arms except the long arm of the homoeologous group 4 chromosome. Aegilops speltoides of the Poaceae family is the most probable donor of the B and G genomes of polyploid Triticum species and also an important source of resistance to diseases and pests of wheat. Previously, we reported the production of a complete set of T aestivum-Ae. speltoides chromosome addition lines and a set of disomic S(B/A)-genome chromosome substitution lines. The isolation of compensating Robertsonian translocations (RobTs) composed of alien chromosome arms translocated to homoeologous wheat chromosome arms is the important next step to exploit the genetic variation of a wild relative of wheat. Here, we report the development of molecular markers specific for the S-genome chromosomes and their use in the isolation of a set of 13 compensating wheat-Ae. speltoides RobTs covering the S genome of Ae. speltoides except for the long arm of chromosome 4S. Most of the RobTs were fully fertile and will facilitate mapping of genes to specific chromosome arms and also will accelerate the introgression of agronomically useful traits from Ae. speltoides into wheat by homologous recombination.
authors: Laboratory of Cell and Chromosome Engineering, College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan, 450002, People's Republic of China.
authors: Wenxuan
authors: W
authors: Liu
authors: Wheat Genetics Resource Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.
authors: Dal-Hoe
authors: DH
authors: Koo
authors: Wheat Genetics Resource Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA. friebe@ksu.edu.
authors: Bernd
authors: http://orcid.org/0000-0002-5419-1565
authors: B
authors: Friebe
authors: Wheat Genetics Resource Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA.
authors: Bikram S
authors: BS
authors: Gill
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D018895:Microsatellite Repeats; D000069600:Plant Breeding; D006109:Poaceae / Q000235:genetics*; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*
chemical_list: D005819:Genetic Markers