title: Alterations in Kernel Proteome after Infection with Fusarium culmorum in Two Triticale Cultivars with Contrasting Resistance to Fusarium Head Blight.
abstract: Highlight: The level of pathogen alpha-amylase and plant beta-amylase activities could be components of plant-pathogen interaction associated with the resistance of triticale to Fusarium head blight. Triticale was used here as a model to recognize new components of molecular mechanism of resistance to Fusarium head blight (FHB) in cereals. Fusarium-damaged kernels (FDK) of two lines distinct in levels of resistance to FHB were applied into a proteome profiling using two-dimensional gel electrophoresis (2-DE) to create protein maps and mass spectrometry (MS) to identify the proteins differentially accumulated between the analyzed lines. This proteomic research was supported by a measurement of alpha- and beta-amylase activities, mycotoxin content, and fungal biomass in the analyzed kernels. The 2-DE analysis indicated a total of 23 spots with clear differences in a protein content between the more resistant and more susceptible triticale lines after infection with Fusarium culmorum. A majority of the proteins were involved in a cell carbohydrate metabolism, stressing the importance of this protein group in a plant response to Fusarium infection. The increased accumulation levels of different isoforms of plant beta-amylase were observed for a more susceptible triticale line after inoculation but these were not supported by a total level of beta-amylase activity, showing the highest value in the control conditions. The more resistant line was characterized by a higher abundance of alpha-amylase inhibitor CM2 subunit and simultaneously a lower activity of alpha-amylase after inoculation. We suggest that the level of pathogen alpha-amylase and plant beta-amylase activities could be components of plant-pathogen interaction associated with the resistance of triticale to FHB.
authors: Institute of Plant Genetics, Polish Academy of Sciences Poznan, Poland.
authors: Dawid
authors: D
authors: Perlikowski
authors: Institute of Plant Genetics, Polish Academy of Sciences Poznan, Poland.
authors: Halina
authors: H
authors: Wiśniewska
authors: Institute of Plant Genetics, Polish Academy of Sciences Poznan, Poland.
authors: Joanna
authors: J
authors: Kaczmarek
authors: Plant Breeding and Acclimatization Institute-National Research Institute Blonie, Poland.
authors: Tomasz
authors: T
authors: Góral
authors: Plant Breeding and Acclimatization Institute-National Research Institute Blonie, Poland.
authors: Piotr
authors: P
authors: Ochodzki
authors: Institute of Plant Genetics, Polish Academy of Sciences Poznan, Poland.
authors: Michał
authors: M
authors: Kwiatek
authors: Institute of Plant Genetics, Polish Academy of Sciences Poznan, Poland.
authors: Maciej
authors: M
authors: Majka
authors: Institute of Plant Genetics, Polish Academy of Sciences Poznan, Poland.
authors: Adam
authors: A
authors: Augustyniak
authors: Institute of Plant Genetics, Polish Academy of Sciences Poznan, Poland.
authors: Arkadiusz
authors: A
authors: Kosmala
keywords: FHB; Fusarium; amylase; cereals; inhibitors; mycotoxins; proteome