title: First detection of benthic cyanobacteria in Lake Baikal producing paralytic shellfish toxins.
abstract: Cyanobacteria were screened from the surface of diseased sponges, stone and bedrock in Lake Baikal for the presence of saxitoxin using enzyme-linked immunosorbent assay. In sequel, eight paralytic shellfish toxin (PST) variants were identified using a MALDI mass spectrometry. Microscopic examination found that Tolypothrix distorta dominated in the biofouling samples. PCR and sequencing detected sxtA gene involved in saxitoxin biosynthesis, thereby providing evidence of the PST producing potential of Baikal cyanobacterial communities inhabiting different substrates.
authors: Limnological Institute, Russian Academy of Sciences, P.O. Box 278, 664033 Irkutsk, Russia. Electronic address: belykh@lin.irk.ru.
authors: Olga I
authors: OI
authors: Belykh
authors: Limnological Institute, Russian Academy of Sciences, P.O. Box 278, 664033 Irkutsk, Russia.
authors: Irina V
authors: IV
authors: Tikhonova
authors: Limnological Institute, Russian Academy of Sciences, P.O. Box 278, 664033 Irkutsk, Russia.
authors: Anton V
authors: AV
authors: Kuzmin
authors: Limnological Institute, Russian Academy of Sciences, P.O. Box 278, 664033 Irkutsk, Russia.
authors: Ekaterina G
authors: EG
authors: Sorokovikova
authors: Limnological Institute, Russian Academy of Sciences, P.O. Box 278, 664033 Irkutsk, Russia.
authors: Galina A
authors: GA
authors: Fedorova
authors: Limnological Institute, Russian Academy of Sciences, P.O. Box 278, 664033 Irkutsk, Russia.
authors: Igor V
authors: IV
authors: Khanaev
authors: Limnological Institute, Russian Academy of Sciences, P.O. Box 278, 664033 Irkutsk, Russia.
authors: Tatyana A
authors: TA
authors: Sherbakova
authors: Limnological Institute, Russian Academy of Sciences, P.O. Box 278, 664033 Irkutsk, Russia.
authors: Oleg A
authors: OA
authors: Timoshkin
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis*; D000458:Cyanobacteria / Q000302:isolation & purification* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D060106:Lakes / Q000382:microbiology*; D057096:Shellfish Poisoning / Q000382:microbiology*; D012800:Siberia
chemical_list: D001427:Bacterial Toxins
keywords: Benthic cyanobacteria; Lake Baikal; Paralytic shellfish toxins; Saxitoxin; Sponge disease; Tolypothrix distorta