title: IKKε inhibits PKC to promote Fascin-dependent actin bundling.
abstract: Signaling molecules have pleiotropic functions and are activated by various extracellular stimuli. Protein kinase C (PKC) is activated by diverse receptors, and its dysregulation is associated with diseases including cancer. However, how the undesired activation of PKC is prevented during development remains poorly understood. We have previously shown that a protein kinase, IKKε, is active at the growing bristle tip and regulates actin bundle organization during Drosophila bristle morphogenesis. Here, we demonstrate that IKKε regulates the actin bundle localization of a dynamic actin cross-linker, Fascin. IKKε inhibits PKC, thereby protecting Fascin from inhibitory phosphorylation. Excess PKC activation is responsible for the actin bundle defects in IKKε-deficient bristles, whereas PKC is dispensable for bristle morphogenesis in wild-type bristles, indicating that PKC is repressed by IKKε in wild-type bristle cells. These results suggest that IKKε prevents excess activation of PKC during bristle morphogenesis.
authors: Laboratory for Morphogenetic Signaling, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan otani@nips.ac.jp shayashi@cdb.riken.jp.
authors: Tetsuhisa
authors: 0000-0002-2557-6722
authors: T
authors: Otani
authors: Laboratory for Morphogenetic Signaling, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan.
authors: Yosuke
authors: Y
authors: Ogura
authors: Electron Microscope Laboratory, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan.
authors: Kazuyo
authors: K
authors: Misaki
authors: Laboratory for Morphogenetic Signaling, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan.
authors: Takuya
authors: T
authors: Maeda
authors: Laboratory for Morphogenetic Signaling, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan.
authors: Akiyo
authors: A
authors: Kimpara
authors: Electron Microscope Laboratory, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan.
authors: Shigenobu
authors: S
authors: Yonemura
authors: Laboratory for Morphogenetic Signaling, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan otani@nips.ac.jp shayashi@cdb.riken.jp.|Department of Biology, Kobe University Graduate School of Science, Kobe, Hyogo 657-8501, Japan.
authors: Shigeo
authors: S
authors: Hayashi
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D051550:I-kappa B Kinase / Q000235:genetics / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D000199:Actins; D002352:Carrier Proteins; D029721:Drosophila Proteins; D008840:Microfilament Proteins; C020324:fascin; D051550:I-kappa B Kinase; D011493:Protein Kinase C
keywords: Actin; Bristle morphogenesis; Drosophila; Fascin; IKKε; PKC; Singed