title: 4-Nonylphenol induced DNA damage and repair in fish, Channa punctatus after subchronic exposure.
abstract: The detection of a possible DNA damaging effect of 4-nonylphenol (NP) after subchronic exposure and repair after cessation of exposure to Channa punctatus is the aim of the present study. Channa punctatus was exposed to different concentrations (0.15 mg/l, 0.10 mg/l, and 0.07 mg/l) of NP along with positive control (ethanol) and negative control (water) for 90 d and after that allowed to recover for 30 d. Comet assay and micronucleus assay were used for the determination of DNA damage and repair by using blood cells. The effect was seen after 30, 60, and 90 d of exposure. Time- and dose-dependent increase in DNA damage was found as revealed by both the end points studied. Evident recovery was observed after 30 d of cessation of exposure. Blood cells were successfully appeared to achieve the restoration of DNA integrity. Hence, the study aimed to improve the knowledge of the genetic hazard to fish associated with NP exposure and provide a wide scope to discover the efficiency of DNA repair system in C. punctatus.
authors: a Department of Zoology , Guru Nanak Dev University , Amritsar , Punjab , India.
authors: Madhu
authors: M
authors: Sharma
authors: a Department of Zoology , Guru Nanak Dev University , Amritsar , Punjab , India.
authors: Pooja
authors: P
authors: Chadha
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000187:drug effects / Q000473:pathology; D020552:Comet Assay; D004249:DNA Damage; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D005399:Fishes / Q000097:blood / Q000235:genetics*; D007928:Lethal Dose 50; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D010636:Phenols / Q000633:toxicity*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D009153:Mutagens; D010636:Phenols; D014874:Water Pollutants, Chemical; C041594:4-nonylphenol
keywords: Micronucleated cell; aberrant cells; comet; nonylphenol (CAS NO 104-40-5)