title: Dehydrocorydaline promotes myogenic differentiation via p38 MAPK activation.
abstract: Muscle regeneration is a coordinated process that involves proliferation and differentiation of muscle progenitor cells. Activation of MyoD is a key event in myogenic differentiation, which is regulated by p38 mitogen‑activated protein kinases (MAPK). In a screen of natural compounds for the enhancement of MyoD activity, dehydrocorydaline (DHC) from the Corydalis tuber was identified. Treatment of C2C12 myoblasts with DHC increased the expression levels of muscle‑specific proteins, including MyoD, myogenin and myosin heavy chain. In addition, C2C12 myoblasts exhibited enhanced multinucleated myotube formation without any cytotoxicity. Treatment with DHC elevated p38 MAPK activation and the interaction of MyoD with an E protein, which is likely to result in activation of MyoD and promotion of myoblast differentiation. Furthermore, defects in differentiation‑induced p38 MAPK activation and myoblast differentiation induced by depletion of the promyogenic receptor protein Cdo in C2C12 myoblasts were restored by DHC treatment. In conclusion, these results indicated that DHC stimulates p38 MAPK activation, which can enhance heterodimerization of MyoD and E proteins, thus resulting in MyoD activation and myoblast differentiation. These findings suggested that DHC may be considered a potential therapeutic compound for the improvement of muscle stem cell regenerative capacity in injured muscle.
authors: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140‑742, Republic of Korea.
authors: Miran
authors: M
authors: Yoo
authors: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140‑742, Republic of Korea.
authors: Sang-Jin
authors: SJ
authors: Lee
authors: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140‑742, Republic of Korea.
authors: Yong Kee
authors: YK
authors: Kim
authors: Department of Biochemistry, College of Pharmacy, Dankook University, Cheonan, Chungcheongnam 330‑714, Republic of Korea.
authors: Dong-Wan
authors: DW
authors: Seo
authors: Department of Oriental Medicine, The Graduate School of Biotechnology, Institute of Life Sciences & Resources, Kyung Hee University, Yongin, Gyeonggi 446‑701, Republic of Korea.
authors: Nam-In
authors: NI
authors: Baek
authors: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140‑742, Republic of Korea.
authors: Jae-Ha
authors: JH
authors: Ryu
authors: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute, Suwon, Gyeonggi 440‑746, Republic of Korea.
authors: Jong-Sun
authors: JS
authors: Kang
authors: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140‑742, Republic of Korea.
authors: Gyu-Un
authors: GU
authors: Bae
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D031311:Corydalis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects*; D051379:Mice; D024510:Muscle Development / Q000187:drug effects*; D017570:MyoD Protein / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; C007232:dehydrocorydalin; D048051:p38 Mitogen-Activated Protein Kinases