title: Electronic Activation of a DNA Nanodevice Using a Multilayer Nanofilm.
abstract: A method to control activation of a DNA nanodevice by supplying a complementary DNA (cDNA) strand from an electro-responsive nanoplatform is reported. To develop functional nanoplatform, hexalayer nanofilm is precisely designed by layer-by-layer assembly technique based on electrostatic interaction with four kinds of materials: Hydrolyzed poly(β-amino ester) can help cDNA release from the film. A cDNA is used as a key building block to activate DNA nanodevice. Reduced graphene oxides (rGOs) and the conductive polymer provide conductivity. In particular, rGOs efficiently incorporate a cDNA in the film via several interactions and act as a barrier. Depending on the types of applied electronic stimuli (reductive and oxidative potentials), a cDNA released from the electrode can quantitatively control the activation of DNA nanodevice. From this report, a new system is successfully demonstrated to precisely control DNA release on demand. By applying more advanced form of DNA-based nanodevices into multilayer system, the electro-responsive nanoplatform will expand the availability of DNA nanotechnology allowing its improved application in areas such as diagnosis, biosensing, bioimaging, and drug delivery.
authors: Laboratory of Functional Nano Films, School of Chemical Engineering and Material Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
authors: Hyejoong
authors: H
authors: Jeong
authors: Chemistry Department, University of Rome Tor Vergata, Via della Ricerca Scientifica, Rome, 00133, Italy.
authors: Simona
authors: S
authors: Ranallo
authors: Chemistry Department, University of Rome Tor Vergata, Via della Ricerca Scientifica, Rome, 00133, Italy.
authors: Marianna
authors: M
authors: Rossetti
authors: Laboratory of Functional Nano Films, School of Chemical Engineering and Material Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
authors: Jiwoong
authors: J
authors: Heo
authors: Laboratory of Organic Materials, School of Chemical Engineering and Material Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
authors: Jooseok
authors: J
authors: Shin
authors: Laboratory of Organic Materials, School of Chemical Engineering and Material Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
authors: Kwangyong
authors: K
authors: Park
authors: Chemistry Department, University of Rome Tor Vergata, Via della Ricerca Scientifica, Rome, 00133, Italy. francesco.ricci@uniroma2.it.
authors: Francesco
authors: F
authors: Ricci
authors: Laboratory of Functional Nano Films, School of Chemical Engineering and Material Science, Chung-Ang University, Seoul, 06974, Republic of Korea. jkhong@cau.ac.kr.
authors: Jinkee
authors: J
authors: Hong
mesh_terms: D004247:DNA / Q000737:chemistry*; D018076:DNA, Complementary / Q000737:chemistry; D004566:Electrodes; D004581:Electronics / Q000295:instrumentation*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry
chemical_list: D018076:DNA, Complementary; D011108:Polymers; C507253:poly(beta-amino ester); D004247:DNA
keywords: DNA nanodevices; electronic activation; graphene oxides; layer-by-layer; multilayer nanofilms