title: The future of NMR-based metabolomics.
abstract: The two leading analytical approaches to metabolomics are mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. Although currently overshadowed by MS in terms of numbers of compounds resolved, NMR spectroscopy offers advantages both on its own and coupled with MS. NMR data are highly reproducible and quantitative over a wide dynamic range and are unmatched for determining structures of unknowns. NMR is adept at tracing metabolic pathways and fluxes using isotope labels. Moreover, NMR is non-destructive and can be utilized in vivo. NMR results have a proven track record of translating in vitro findings to in vivo clinical applications.
authors: Biochemistry Department, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA. Electronic address: jmarkley@wisc.edu.
authors: John L
authors: JL
authors: Markley
authors: Department of Chemistry & Biochemistry, The Ohio State University, 151 W. Woodruff Ave., Columbus, OH 43210, USA; Department of Biological Chemistry & Pharmacology, The Ohio State University, 151 W. Woodruff Ave., Columbus, OH 43210, USA.
authors: Rafael
authors: R
authors: Brüschweiler
authors: Department of Genetics and Biochemistry, Institute of Bioinformatics and Complex Carbohydrate Center, University of Georgia, 315 Riverbend Rd, Athens, GA 30602, USA.
authors: Arthur S
authors: AS
authors: Edison
authors: Biochemistry Department, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 53706, USA.
authors: Hamid R
authors: HR
authors: Eghbalnia
authors: Department of Chemistry, University of Nebraska-Lincoln, 722 Hamilton Hall, Lincoln, NE 68588, USA.
authors: Robert
authors: R
authors: Powers
authors: Department of Anesthesiology & Pain Medicine, 850 Republican St, University of Washington, Seattle, WA 98109, USA.
authors: Daniel
authors: D
authors: Raftery
authors: Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E8; Department of Computing Science, University of Alberta, Edmonton, AB, Canada T6G 2E8.
authors: David S
authors: DS
authors: Wishart
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D053858:Metabolic Networks and Pathways; D055432:Metabolomics / Q000379:methods*
chemical_list: D015415:Biomarkers