title: Novel Foxp3(-) IL-10(-) Regulatory T-cells Induced by B-Cells Alleviate Intestinal Inflammation in Vivo.
abstract: Recent studies have revealed various Foxp3(-) regulatory T (Treg) cell subsets effectively protect mice from colitis. In the present study, we demonstrated that B cells induced a particular subset of regulatory T (Treg-of-B) cells, expressing programmed cell death 1 (PD-1), inducible costimulator (ICOS), lymphocyte-activation gene 3 (LAG3), glucocorticoid-induced tumor necrosis factor receptor (GITR), and OX-40, did not express Foxp3. Treg-of-B cells produced abundant levels of IL-10 and low levels of IL-4 and TGF-β. Adoptive transfer of Treg-of-B cells protected mice from CD4(+)CD45RB(hi) T-cell-induced colitis, including infiltration of leukocytes, depletion of goblet cells, epithelial hyperplasia, and inhibition of Th1 and Th17 cytokines. These features were similar to IL-10-producing type 1 regulatory T (Tr1) cells; however, IL-10-deficient Treg-of-B cells maintained their suppressive function in vitro as well as in vivo, while the regulation of Tr1 cells depended on IL-10. In conclusion, Treg-of-B cells protected against experimental colitis through an IL-10-independent mechanism. We reported a novel subpopulation of regulatory T cells was different from conventional Foxp3(+) Treg and IL-10-producing Tr1 cells.
authors: Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, No. 1, Chang-Te Street, Taipei 10048, Taiwan.
authors: Tzu-Yu
authors: TY
authors: Shao
authors: Graduate Institute of Immunology, College of Medicine, National Taiwan University, No. 1, Chang-Te Street, Taipei 10048, Taiwan.
authors: Ling-Hui
authors: LH
authors: Hsu
authors: Graduate Institute of Immunology, College of Medicine, National Taiwan University, No. 1, Chang-Te Street, Taipei 10048, Taiwan.
authors: Chien-Hui
authors: CH
authors: Chien
authors: Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, No. 1, Chang-Te Street, Taipei 10048, Taiwan.|Graduate Institute of Immunology, College of Medicine, National Taiwan University, No. 1, Chang-Te Street, Taipei 10048, Taiwan.|Department of Medical Research, National Taiwan University Hospital, No. 7 Chung-Shan South Road, Taipei 10002, Taiwan.
authors: Bor-Luen
authors: BL
authors: Chiang
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D002450:Cell Communication / Q000276:immunology; D003092:Colitis / Q000235:genetics / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation; D053450:Glucocorticoid-Induced TNFR-Related Protein / Q000235:genetics / Q000276:immunology; D020397:Goblet Cells / Q000276:immunology / Q000473:pathology; D060889:Inducible T-Cell Co-Stimulator Protein / Q000235:genetics / Q000276:immunology; D016753:Interleukin-10 / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D007422:Intestines / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D061026:Programmed Cell Death 1 Receptor / Q000235:genetics / Q000276:immunology; D053262:Receptors, OX40 / Q000235:genetics / Q000276:immunology; D012720:Severity of Illness Index; D015398:Signal Transduction; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000473:pathology / Q000637:transplantation; D018417:Th1 Cells / Q000276:immunology / Q000473:pathology; D058504:Th17 Cells / Q000276:immunology / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000276:immunology; D000093322:Lymphocyte Activation Gene 3 Protein
chemical_list: D015703:Antigens, CD; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D053450:Glucocorticoid-Induced TNFR-Related Protein; C556062:IL10 protein, mouse; C556694:Icos protein, mouse; D060889:Inducible T-Cell Co-Stimulator Protein; C491383:Pdcd1 protein, mouse; D061026:Programmed Cell Death 1 Receptor; D053262:Receptors, OX40; C106544:Tnfrsf18 protein, mouse; C505717:Tnfrsf4 protein, mouse; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D015847:Interleukin-4; D000093322:Lymphocyte Activation Gene 3 Protein; C000720327:Lag3 protein, mouse