title: Distance-of-Flight Mass Spectrometry: What, Why, and How?
abstract: Distance-of-flight mass spectrometry (DOFMS) separates ions of different mass-to-charge (m/z) by the distance they travel in a given time after acceleration. Like time-of-flight mass spectrometry (TOFMS), separation and mass assignment are based on ion velocity. However, DOFMS is not a variant of TOFMS; different methods of ion focusing and detection are used. In DOFMS, ions are driven orthogonally, at the detection time, onto an array of detectors parallel to the flight path. Through the independent detection of each m/z, DOFMS can provide both wider dynamic range and increased throughput for m/z of interest compared with conventional TOFMS. The iso-mass focusing and detection of ions is achieved by constant-momentum acceleration (CMA) and a linear-field ion mirror. Improved energy focus (including turn-around) is achieved in DOFMS, but the initial spatial dispersion of ions remains unchanged upon detection. Therefore, the point-source nature of surface ionization techniques could put them at an advantage for DOFMS. To date, three types of position-sensitive detectors have been used for DOFMS: a microchannel plate with a phosphorescent screen, a focal plane camera, and an IonCCD array; advances in detector technology will likely improve DOFMS figures-of-merit. In addition, the combination of CMA with TOF detection has provided improved resolution and duty factor over a narrow m/z range (compared with conventional, single-pass TOFMS). The unique characteristics of DOFMS can enable the intact collection of large biomolecules, clusters, and organisms. DOFMS might also play a key role in achieving the long-sought goal of simultaneous MS/MS. Graphical Abstract ᅟ.
authors: Department of Chemistry, Indiana University, Bloomington, IN, 47405, USA.|Perkin Elmer, Shelton, CT, 06484, USA.
authors: Elise A
authors: EA
authors: Dennis
authors: Department of Chemistry, Indiana University, Bloomington, IN, 47405, USA.|Department of Chemistry and Applied Biosciences, ETH Zurich, 8093, Zurich, Switzerland.
authors: Alexander W
authors: AW
authors: Gundlach-Graham
authors: Department of Chemistry, State University of New York at Buffalo, Buffalo, NY, 14260, USA.
authors: Steven J
authors: SJ
authors: Ray
authors: Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, NM, 87131, USA.
authors: Christie G
authors: CG
authors: Enke
authors: Department of Chemistry, Indiana University, Bloomington, IN, 47405, USA. hieftje@indiana.edu.
authors: Gary M
authors: GM
authors: Hieftje
keywords: Distance-of-flight; Inductively coupled plasma; IonCCD; MALDI; Matrix-assisted laser-desorption ionization; Sector field mass spectrometry; Time-of-flight