title: Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens.
abstract: The intrinsic limitations of 2D monolayer cell culture models have prompted the development of 3D cell culture model systems for in vitro studies. Multicellular tumor spheroid (MCTS) models closely simulate the pathophysiological milieu of solid tumors and are providing new insights into tumor biology as well as differentiation, tissue organization, and homeostasis. They are straightforward to apply in high-throughput screens and there is a great need for the development of reliable and robust 3D spheroid-based assays for high-throughput RNAi screening for target identification and cell signaling studies highlighting their potential in cancer research and treatment. In this chapter we describe a stringent standard operating procedure for the use of MCTS for high-throughput RNAi screens.
authors: Division of Cancer Medicine, Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, 77054, USA.
authors: LaKesla R
authors: LR
authors: Iles
authors: Division of Cancer Medicine, Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, TX, 77054, USA. gbarthol@mdanderson.org.
authors: Geoffrey A
authors: GA
authors: Bartholomeusz
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D034622:RNA Interference; D018874:Spheroids, Cellular; D014162:Transfection / Q000379:methods
keywords: High throughput; Lipid transfection; Robotics; Spheroid; siRNA