title: Lipid production through simultaneous utilization of glucose, xylose, and L-arabinose by Pseudozyma hubeiensis: a comparative screening study.
abstract: Co-fermentation of glucose, xylose and L-arabinose from lignocellulosic biomass by an oleaginous yeast is anticipated as a method for biodiesel production. However, most yeasts ferment glucose first before consuming pentoses, due to glucose repression. This preferential utilization results in delayed fermentation time and lower productivity. Therefore, co-fermentation of lignocellulosic sugars could achieve cost-effective conversion of lignocellulosic biomass to microbial lipid. Comprehensive screening of oleaginous yeasts capable of simultaneously utilizing glucose, xylose, and L-arabinose was performed by measuring the concentration of sugars remaining in the medium and of lipids accumulated in the cells. We found that of 1189 strains tested, 12 had the ability to co-ferment the sugars. The basidiomycete yeast Pseudozyma hubeiensis IPM1-10, which had the highest sugars consumption rate of 94.1 %, was selected by culturing in a batch culture with the mixed-sugar medium. The strain showed (1) simultaneous utilization of all three sugars, and (2) high lipid-accumulating ability. This study suggests that P. hubeiensis IPM1-10 is a promising candidate for second-generation biodiesel production from hydrolysate of lignocellulosic biomass.
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.
authors: Ayumi
authors: A
authors: Tanimura
authors: Japan Collection of Microorganisms, RIKEN BioResource Center, Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.
authors: Masako
authors: M
authors: Takashima
authors: Department of Microbiology, Meiji Pharmaceutical University, Noshio, Kiyose, Tokyo, 204-8588, Japan.
authors: Takashi
authors: T
authors: Sugita
authors: Japan Collection of Microorganisms, RIKEN BioResource Center, Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.
authors: Rikiya
authors: R
authors: Endoh
authors: Japan Collection of Microorganisms, RIKEN BioResource Center, Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.
authors: Moriya
authors: M
authors: Ohkuma
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.
authors: Shigenobu
authors: S
authors: Kishino
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.
authors: Jun
authors: J
authors: Ogawa
authors: Faculty of Agriculture, Ryukoku University, Seta Oe-cho, Otsu, Shiga, 520-2194, Japan. shima@agr.ryukoku.ac.jp.
authors: Jun
authors: J
authors: Shima
keywords: Fatty acids; L-arabinose; Oleaginous yeast; Pseudozyma hubeiensis; Xylose