title: Growth and metabolic profiling of the novel thermophilic bacterium Thermoanaerobacter sp. strain YS13.
abstract: A strictly anaerobic, thermophilic bacterium, designated strain YS13, was isolated from a geothermal hot spring. Phylogenetic analysis using the 16S rRNA genes and cpn60 UT genes suggested strain YS13 as a species of Thermoanaerobacter. Using cellobiose or xylose as carbon source, YS13 was able to grow over a wide range of temperatures (45-70 °C), and pHs (pH 5.0-9.0), with optimum growth at 65 °C and pH 7.0. Metabolic profiling on cellobiose, glucose, or xylose in 1191 medium showed that H2, CO2, ethanol, acetate, and lactate were the major metabolites. Lactate was the predominant end product from glucose or cellobiose fermentations, whereas H2 and acetate were the dominant end products from xylose fermentation. The metabolic balance shifted away from ethanol to H2, acetate, and lactate when YS13 was grown on cellobiose as temperatures increased from 45 to 70 °C. When YS13 was grown on xylose, a metabolic shift from lactate to H2, CO2, and acetate was observed in cultures as the temperature of incubation increased from 45 to 65 °C, whereas a shift from ethanol and CO2 to H2, acetate, and lactate was observed in cultures incubated at 70 °C.
authors: a Department of Food Science, Huazhong Agricultural University, Wuhan, China.|d Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 3N3, Canada.
authors: Tingting
authors: T
authors: Peng
authors: a Department of Food Science, Huazhong Agricultural University, Wuhan, China.
authors: Siyi
authors: S
authors: Pan
authors: b Biorefining Research Institute, Lakehead University, Thunder Bay, ON P7B 5Z5, Canada.
authors: Lew P
authors: LP
authors: Christopher
authors: c Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 3N3, Canada.
authors: Richard
authors: R
authors: Sparling
authors: d Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 3N3, Canada.
authors: David B
authors: DB
authors: Levin
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002475:Cellobiose / Q000378:metabolism; D045482:Hot Springs / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D046968:Thermoanaerobacter / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D012336:RNA, Ribosomal, 16S; D002475:Cellobiose
keywords: bactéries thermophile anaérobie; conditions de croissance optimales; metabolic end products; microbial metabolism; métabolisme microbien; optimal growth conditions; produits métaboliques finaux; thermophilic anaerobic bacteria