title: Evaluation of nitric oxide production and proliferation activity of recombinant Bacterioferritin of Helicobacter pylori on macrophages.
abstract: BACKGROUND
  Helicobacter pylori is a specific pathogen found in the human stomach. The Bacterioferritin of Helicobacter pylori is a major virulence factor of this pathogen which little is known about its effect on immune system. The aim of this study is to assess the effect of recombinant Bacterioferritin Helicobacter pylori on the production of nitric oxide (NO) and the activity and viability of macrophages derived from mice peritoneal.
  
  
  METHODS
  The Bacterioferritin protein of Helicobacter pylori was cloned and purified. Mice peritoneal macrophages were purified and cultured. Different concentrations of recombinant protein were used to stimulate macrophages which had received LPS simultaneously. Cell survival and nitric oxide (NO) production were measured subsequently.
  
  
  RESULTS
  Our results elucidated that the recombinant protein induced a significant NO production at a dose of 30 μg/ml (P < 0.01) compared to the control which was accompanied by increasing in the viability of macrophages at dosage of 30 μg/ml.
  
  
  CONCLUSIONS
  According to our findings, recombinant protein stimulates peritoneal macrophages to produce NO and does not have cytotoxic effect. Therefore, it is suggested that recombinant Bacterioferritin can be studied further as a vaccine candidate for Immunotherapy purposes.
authors: Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran. Electronic address: N_soleimani@sbu.ac.ir.
authors: Neda
authors: N
authors: Soleimani
authors: Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Ashraf
authors: A
authors: Mohabati Mobarez
authors: Department of Biochemistry, School of Medicine, Iran University of Medical Science, Tehran, Iran.
authors: Masoumeh
authors: M
authors: Tavakoli-Yaraki
authors: Molecular Genetics, Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Baharak
authors: B
authors: Farhangi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003573:Cytochrome b Group / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D005293:Ferritins / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000382:microbiology*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000633:toxicity
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; D011994:Recombinant Proteins; D009569:Nitric Oxide; D005293:Ferritins; C018032:bacterioferritin
keywords: Helicobacter pylori; Nitric oxide; Recombinant protein; Viability