title: Study on glyco-modification of endostatin-derived synthetic peptide endostatin2 (ES2) by soluble chitooligosaccharide.
abstract: Soluble O-(2-hydroxyl)propyl-3-trimethyl ammonium chitooligosaccharide chloride (HTCOSC) was covalently conjugated to the 11-amino-acid peptide derived from amino terminus of endostatin (endostatin2, ES2, IVRRADRAAVP) to overcome its poor stability, low cell affinity and instable activity. Nuclear magnetic resonance spectroscopy and mass spectrometry was used to study the structure and molecular weight information. The anti-angiogenic activities were evaluated using cell counting Kit-8 assay, flow cytometry assay, wounding migration assay, transwell migration assay, chicken chorioallantoic membrane (CAM) assay and zebra fish angiogenesis assay. In contrast with ES2, the novel carbohydrate-polymer HTCOSC-ES2 displayed improved heat stability, higher cell affinity, better inhibition on endothelial cell proliferation, tube formation, 2-dimensional and 3-dimensional migration in vitro. According to the evaluation in CAM and zebra fish, HTCOSC-ES2 also displayed better anti-angiogenic activity than ES2 in vivo. These results indicate that HTCOSC has good properties as potential candidate for protein/peptide modifier and HTCOSC-ES2 has good potential in angiogenesis related diseases treatment.
authors: National Glycoengineering Research Center, Shandong University, Jinan 250012, China; Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan 250012, China.
authors: Yang
authors: Y
authors: Yu
authors: National Glycoengineering Research Center, Shandong University, Jinan 250012, China; Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan 250012, China.
authors: Feng
authors: F
authors: Sun
authors: College of Life Sciences, Qufu Normal University, Qufu 273165, China.
authors: Congcong
authors: C
authors: Zhang
authors: National Glycoengineering Research Center, Shandong University, Jinan 250012, China; Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan 250012, China.
authors: Zhendong
authors: Z
authors: Wang
authors: College of Life Sciences, Qufu Normal University, Qufu 273165, China.
authors: Jinfeng
authors: J
authors: Liu
authors: National Glycoengineering Research Center, Shandong University, Jinan 250012, China; Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan 250012, China. Electronic address: hainingtan@sdu.edu.cn.
authors: Haining
authors: H
authors: Tan
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002642:Chick Embryo; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D048271:Chitosan; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004625:Embryo, Nonmammalian / Q000187:drug effects; D043169:Endostatins / Q000737:chemistry* / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009844:Oligosaccharides; D010446:Peptide Fragments / Q000737:chemistry* / Q000494:pharmacology*; D055550:Protein Stability; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015027:Zebrafish / Q000196:embryology / Q000235:genetics
chemical_list: C000607498:AP25 peptide; D020533:Angiogenesis Inhibitors; D043169:Endostatins; D009844:Oligosaccharides; D010446:Peptide Fragments; C493484:oligochitosan; D002686:Chitin; D048271:Chitosan
keywords: Anti-angiogenic activities; Chitooligosaccharide; Endostatin-derived peptide; Glyco-modification; Heat stability