title: Resveratrol inhibits steroidogenesis in human fetal adrenocortical cells at the end of first trimester.
abstract: Resveratrol has a diverse array of healthful effects on metabolic parameters in different experimental paradigms but has also potential to inhibit steroidogenesis in rodent adrenals. The aim of the present study was to characterize the effects of resveratrol on human fetal adrenal steroidogenesis at gestational weeks (GW) 9-12.
  
  
  
  Adrenals from aborted fetuses (GW10-12) were used to prepare primary cultures of human fetal adrenocortical cells (HFAC). HFAC were treated in the presence or absence of ACTH (10 ng/mL) with or without resveratrol (10 μM) for 24 h. The production of steroids by HFAC was analyzed by gas and liquid chromatography coupled to tandem/mass spectrometry. The expression of steroidogenic enzymes at GW 9-12 was quantified by automated Western blotting. We observed that resveratrol significantly suppressed synthesis of dehydroepiandrosterone (DHEA), androstenedione and 11-deoxicortisol by ACTH-activated and unstimulated HFAC, which was associated with inhibition of the activities and expression of cytochromes 17α-hydroxylase/17,20 lyase (CYP17) and 21-hydroxylase (CYP21) in these fetal adrenocortical cells.
  
  
  
  Our in vitro findings on the sensitivity of human fetal adrenal steroidogenesis to resveratrol at GW9-12 suggest that intake of this polyphenol at high doses by women who are at early stages of pregnancy is undesirable.
authors: Department of Women's and Children's Health, Pediatric Endocrinology Unit, Karolinska Institutet & Karolinska University Hospital, Stockholm, Sweden.
authors: Iuliia
authors: I
authors: Savchuk
authors: LUNAM Université, École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (Oniris), Laboratoire d'Étude des Résidus et Contaminants dans les aliments (LABERCA), USC INRA, Nantes, France.
authors: Marie-Line
authors: ML
authors: Morvan
authors: Department of Growth and Reproduction, Rigshospitalet, Copenhagen University Hospital, Faculty of Health and Medical Sciences, University of Copenhagen, Novo Nordisk A/S Måløv, Denmark.
authors: Tue
authors: T
authors: Søeborg
authors: LUNAM Université, École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (Oniris), Laboratoire d'Étude des Résidus et Contaminants dans les aliments (LABERCA), USC INRA, Nantes, France.
authors: Jean-Philippe
authors: JP
authors: Antignac
authors: Department of Obstetrics and Gynecology, Karolinska Institute & University Hospital, Stockholm, Sweden.
authors: Kristina
authors: K
authors: Gemzell-Danielsson
authors: LUNAM Université, École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (Oniris), Laboratoire d'Étude des Résidus et Contaminants dans les aliments (LABERCA), USC INRA, Nantes, France.
authors: Bruno
authors: B
authors: Le Bizec
authors: Department of Women's and Children's Health, Pediatric Endocrinology Unit, Karolinska Institutet & Karolinska University Hospital, Stockholm, Sweden.
authors: Olle
authors: O
authors: Söder
authors: Department of Women's and Children's Health, Pediatric Endocrinology Unit, Karolinska Institutet & Karolinska University Hospital, Stockholm, Sweden.
authors: Konstantin
authors: K
authors: Svechnikov
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D002478:Cells, Cultured; D000072497:Cytochrome P450 Family 21 / Q000037:antagonists & inhibitors / Q000378:metabolism; D005260:Female; D006801:Humans; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D000077185:Resveratrol; D013254:Steroid 17-alpha-Hydroxylase / Q000037:antagonists & inhibitors / Q000378:metabolism; D013256:Steroids / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D013256:Steroids; D013267:Stilbenes; D000072497:Cytochrome P450 Family 21; D013254:Steroid 17-alpha-Hydroxylase; D000077185:Resveratrol
keywords: Human fetal adrenocortical cells; Resveratrol; Steroidogenesis; Steroidogenic enzymes