title: MiR-590-3p regulates osteogenic differentiation of human mesenchymal stem cells by regulating APC gene.
abstract: The present study aimed to investigate miR-590-3p's role in osteogenic differentiation of human mesenchymal stem cells (hMSC). HMSC were cultured and transfected with empty vector, miR-590-3p vector to construct control hMSC and miR-590-3p overexpression hMSC. MiR-590-3p suppressed hMSC was created with oligonucleotide transient transfection. All 3 groups of cells were analyzed for their osteogenic level as well as Wnt/β-catenin signaling pathway activities. The results of alizarin red staining indicated that overexpression of miR-590-3p significantly enhanced mineralized deposition. Osteogenic markers including ALP, OC and SOST were also up-regulated. The result of dual-luciferase and western blot analysis indicated that miR-590-3p bound to 3'UTR of APC mRNA selectively. By suppressing mRNA level of APC, over-expressed miR-590-3p stabilized β-catenin and increased the transcription activities of downstream target genes. These result suggested that miR-590-3p can promote osteogenic differentiation via suppressing APC expression and stabilizing β-catenin.
authors: Guangdong Provincial Stomatological Hospital, Southern Medical University, No. 366, Jiangnan Avenue South, Haizhu District, Guangzhou, Guangdong 510000, China; Affiliated Stomatological Hospital of Foshan University, No. 5, Hebin Road, Chancheng District, Foshan, Guangdong 528000, China.
authors: Siyuan
authors: S
authors: Wu
authors: Guangdong Provincial Stomatological Hospital, Southern Medical University, No. 366, Jiangnan Avenue South, Haizhu District, Guangzhou, Guangdong 510000, China.
authors: Weizhen
authors: W
authors: Liu
authors: Guangdong Provincial Stomatological Hospital, Southern Medical University, No. 366, Jiangnan Avenue South, Haizhu District, Guangzhou, Guangdong 510000, China. Electronic address: leizhougdps@sina.com.
authors: Lei
authors: L
authors: Zhou
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics*; D005786:Gene Expression Regulation; D017491:Genes, APC; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010012:Osteogenesis / Q000235:genetics*; D060449:Wnt Signaling Pathway / Q000235:genetics
chemical_list: C568221:MIRN590 microRNA, human; D035683:MicroRNAs; D008156:Luciferases
keywords: Micro RNA; Osteogenic differentiation; Stem cells; Tissue engineering