title: Sensitive QD@SiO2-based immunoassay for triplex determination of cereal-borne mycotoxins.
abstract: A sensitive tool for simultaneous quantitative determination of three analytes in one single well of a microtiter plate is shown for the first time. The developed technique is based on use of colloidal quantum dot enrobed into a silica shell (QD@SiO2) derivatives as a highly responsive label. Silica-coated quantum dots were prepared and subsequently modified via the co-hydrolysis with tetraethylorthosilicate (TEOS) and various organosilane reagents. Different surface modification schemes were compared in terms of applicability of the obtained particles for the multiplex immunoassay, e.g. stability and simplicity of their conjugation with biomolecules. As model system a multiplex immunosorbent assay for screening of three mycotoxins (deoxynivalenol, zearalenone and aflatoxin B1) in cereal-based products was realized via a co-immobilization of three different specific antibodies (anti- deoxynivalenol, anti-zearalenone and anti-aflatoxin B1) in one single well of a microtiter plate. Mycotoxins were simultaneously determined by labelling their conjugates with QD@SiO2 emitting in different parts of the visible spectrum. The limits of detection for the simultaneous determination were 6.1 and 5.3, 5.4 and 4.1, and 2.6 and 1.9µgkg(-1) for deoxynivalenol, zearalenone and aflatoxin B1 in maize and wheat, respectively. As confirmatory method, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used.
authors: Faculty of Pharmaceutical Sciences, Department of Bioanalysis, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, Ghent, Belgium. Electronic address: beloglazovanv@gmail.com.
authors: Natalia V
authors: NV
authors: Beloglazova
authors: Faculty of Pharmaceutical Sciences, Department of Bioanalysis, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, Ghent, Belgium.
authors: Astrid
authors: A
authors: Foubert
authors: Saratov State University, Chemistry Faculty, Department of General and Inorganic Chemistry, Astrakhanskaya 83, 410012 Saratov, Russia.
authors: Anna
authors: A
authors: Gordienko
authors: Physics and Chemistry of Nanostructures, Ghent University, Ghent, Belgium; Center for Nano and Biophotonics, Ghent University, Ghent, Belgium.
authors: Mickael D
authors: MD
authors: Tessier
authors: Physics and Chemistry of Nanostructures, Ghent University, Ghent, Belgium; Center for Nano and Biophotonics, Ghent University, Ghent, Belgium.
authors: Tangi
authors: T
authors: Aubert
authors: Physics and Chemistry of Nanostructures, Ghent University, Ghent, Belgium; Center for Nano and Biophotonics, Ghent University, Ghent, Belgium.
authors: Emile
authors: E
authors: Drijvers
authors: Saratov State University, Chemistry Faculty, Department of General and Inorganic Chemistry, Astrakhanskaya 83, 410012 Saratov, Russia.
authors: Irina
authors: I
authors: Goryacheva
authors: Physics and Chemistry of Nanostructures, Ghent University, Ghent, Belgium; Center for Nano and Biophotonics, Ghent University, Ghent, Belgium.
authors: Zeger
authors: Z
authors: Hens
authors: Faculty of Pharmaceutical Sciences, Department of Bioanalysis, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, Ghent, Belgium.
authors: Sarah
authors: S
authors: De Saeger
mesh_terms: D002853:Chromatography, Liquid; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay; D007074:Immunoglobulin G / Q000276:immunology; D009183:Mycotoxins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D045663:Quantum Dots / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D009183:Mycotoxins; D012822:Silicon Dioxide
keywords: Immunoassay; Multiplex; Mycotoxins; QD@SiO2; Quantum dots; Rapid test