title: Improvement of common carp (Cyprinus carpio) sperm cryopreservation using a programable freezer.
abstract: The applicability of a programmable freezer for the increased-scale cryopreservation of common carp sperm was investigated. The effect of different equilibration times, cryopreservation methods, extenders, dilution ratios, activating solutions on the post-thaw motility of common carp sperm was investigated. The suitable post-thaw storage time-interval as well as fertilizing capacity of cryopreserved sperm was also examined. The motility, curvilinear velocity (VCL) and straightness (STR) values did not decrease significantly during 60min of equilibration neither in equilibrated nor thawed groups. Motility parameters of thawed sperm were similar using a conventional cryopreservation technique using a polystyrene box [motility (33%), VCL (47μm/s) and STR (88%)] and a programmable freezer: [motility (32%), VCL (54μm/s) and STR (89%)]. The highest motility and VCL was measured with a sugar based extender (grayling extender) at a ratio 1:9 (motility: 52%, VCL: 76μm/s) and 1:20 (motility: 49%, VCL: 76μm/s). The activating solution for cyprinids (ASC) could prolong sperm movement up for 2min. A storage time of six hours following thawing did not have a significant effect on the motility parameters of thawed carp sperm. Agglutination was observed during cryopreservation of an elevated volume of sperm whereas motility 47%, VCL 62μm/s and STR 91% were measured after thawing. Fertilization rate with thawed sperm (32%) was significantly lower compared to the control group (73%). According to our results, the developed method using a programmable freezer is suitable for the cryopreservation of elevated number of straws. However, carp sperm agglutination during freezing may have a negative effect on the fertilizing capacity.
authors: Department of Aquaculture, Szent István University, Páter Károly u. 1., H-2100 Gödöllő, Hungary. Electronic address: Bernath.Gergely@mkk.szie.hu.
authors: Gergely
authors: G
authors: Bernáth
authors: Department of Aquaculture, Szent István University, Páter Károly u. 1., H-2100 Gödöllő, Hungary; UR AFPA, Université de Lorraine-INRA, 2 Avenue de la Forêt de Haye, BP 172, 54505 Vandœuvre-lès-Nancy, France.
authors: Daniel
authors: D
authors: Żarski
authors: Department of Aquaculture, Szent István University, Páter Károly u. 1., H-2100 Gödöllő, Hungary.
authors: Eszter
authors: E
authors: Kása
authors: Department of Aquaculture, Szent István University, Páter Károly u. 1., H-2100 Gödöllő, Hungary.
authors: Ádám
authors: Á
authors: Staszny
authors: Department of Aquaculture, Szent István University, Páter Károly u. 1., H-2100 Gödöllő, Hungary.
authors: Levente
authors: L
authors: Várkonyi
authors: Department of Aquaculture, Szent István University, Páter Károly u. 1., H-2100 Gödöllő, Hungary.
authors: Tímea
authors: T
authors: Kollár
authors: Department of Aquaculture, Szent István University, Páter Károly u. 1., H-2100 Gödöllő, Hungary.
authors: Árpád
authors: Á
authors: Hegyi
authors: Department of Aquaculture, Szent István University, Páter Károly u. 1., H-2100 Gödöllő, Hungary.
authors: Zoltán
authors: Z
authors: Bokor
authors: Department of Aquaculture, Szent István University, Páter Károly u. 1., H-2100 Gödöllő, Hungary.
authors: Béla
authors: B
authors: Urbányi
authors: Department of Aquaculture, Szent István University, Páter Károly u. 1., H-2100 Gödöllő, Hungary.
authors: Ákos
authors: Á
authors: Horváth
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002347:Carps / Q000378:metabolism*; D015925:Cryopreservation / Q000295:instrumentation* / Q000379:methods*; D005260:Female; D005306:Fertilization; D005615:Freezing; D008297:Male; D012662:Semen Preservation / Q000295:instrumentation* / Q000379:methods*; D012996:Solutions; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D012996:Solutions
keywords: Common carp; Increased-scale; Motility assessment; Programmable freezer; Sperm cryopreservation; Standardization