title: Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction.
abstract: BACKGROUND
  Although rare, circulating endothelial and progenitor cells could be considered as markers of endothelial damage and repair potential, possibly predicting the severity of cardiovascular manifestations. A number of studies highlighted the role of these cells in age-related diseases, including those characterized by ectopic calcification. Nevertheless, their use in clinical practice is still controversial, mainly due to difficulties in finding reproducible and accurate methods for their determination.
  
  
  METHODS
  Circulating mature cells (CMC, CD45-, CD34+, CD133-) and circulating progenitor cells (CPC, CD45dim, CD34bright, CD133+) were investigated by polychromatic high-speed flow cytometry to detect the expression of endothelial (CD309+) or osteogenic (BAP+) differentiation markers in healthy subjects and in patients affected by peripheral vascular manifestations associated with ectopic calcification.
  
  
  RESULTS
  This study shows that: 1) polychromatic flow cytometry represents a valuable tool to accurately identify rare cells; 2) the balance of CD309+ on CMC/CD309+ on CPC is altered in patients affected by peripheral vascular manifestations, suggesting the occurrence of vascular damage and low repair potential; 3) the increase of circulating cells exhibiting a shift towards an osteoblast-like phenotype (BAP+) is observed in the presence of ectopic calcification.
  
  
  CONCLUSIONS
  Differences between healthy subjects and patients with ectopic calcification indicate that this approach may be useful to better evaluate endothelial dysfunction in a clinical context.
authors: Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors: Federica
authors: F
authors: Boraldi
authors: Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors: Angelica
authors: A
authors: Bartolomeo
authors: Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors: Sara
authors: S
authors: De Biasi
authors: Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors: Stefania
authors: S
authors: Orlando
authors: Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors: Sonia
authors: S
authors: Costa
authors: Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors: Andrea
authors: A
authors: Cossarizza
authors: Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 287, Modena, Italy.
authors: Daniela
authors: D
authors: Quaglino
mesh_terms: D018952:Antigens, CD34 / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002318:Cardiovascular Diseases / Q000097:blood / Q000175:diagnosis; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000097:blood
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; C000588866:KDR protein, human; D040301:Vascular Endothelial Growth Factor Receptor-2