title: MicroRNA-588 suppresses tumor cell migration and invasion by targeting GRN in lung squamous cell carcinoma.
abstract: MicroRNAs (miRNAs) have been demonstrated to be critical in regulating tumor development and progression. The present study investigated the expression of miR‑588 using reverse transcription‑quantitative polymerase chain reaction analysis in 85 cases of lung squamous cell carcinoma (SCC), and observed the correlation between the expression of miR‑588 with clinical pathologic features. The results indicated that the expression of miR‑588 was predominantly lower in the tumor samples, compared with non‑tumorous samples, and was negatively associated with tumor stages and lymph node invasion. The present study also examined the significance of the expression of miR‑588 in SCC using gain‑ and loss‑of‑function analyses. It was found that miR‑588 inhibited tumor cell migration and invasion. In addition, it was revealed that the overexpression of miR‑588 in SCC cells reduced the mRNA and protein levels of progranulin (GRN), whereas miR‑588 silencing increased the expression of GRN. A luciferase activity assay showed that miR‑588 was able to directly bind to the 3'untranslated region of GRN and regulate its expression. Furthermore, it was found that the expression of GRN was inversely correlated with the expression of miR‑588 in 85 paired SCC samples. These results indicated that GRN was involved in the miR-588-mediated suppressive functions in the progression of SCC.
authors: Department of Geriatrics, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors: Li
authors: L
authors: Qian
authors: Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200032, P.R. China.
authors: Longlong
authors: L
authors: Lin
authors: Department of Geriatrics, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors: Yufeng
authors: Y
authors: Du
authors: Department of Geriatrics, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors: Xiaoyan
authors: X
authors: Hao
authors: Department of Geriatrics, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors: Yuze
authors: Y
authors: Zhao
authors: Department of Geriatrics, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
authors: Xuejun
authors: X
authors: Liu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002465:Cell Movement; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D000077153:Progranulins
chemical_list: C492314:GRN protein, human; D036341:Intercellular Signaling Peptides and Proteins; C000616791:MIRN588 microRNA, human; D035683:MicroRNAs; D000077153:Progranulins