title: Oral Vaccination with Attenuated Salmonella typhimurium-Delivered TsPmy DNA Vaccine Elicits Protective Immunity against Trichinella spiralis in BALB/c Mice.
abstract: Our previous studies showed that Trichinella spiralis paramyosin (TsPmy) is an immunomodulatory protein that inhibits complement C1q and C8/C9 to evade host complement attack. Vaccination with recombinant TsPmy protein induced protective immunity against T. spiralis larval challenge. Due to the difficulty in producing TsPmy as a soluble recombinant protein, we prepared a DNA vaccine as an alternative approach in order to elicit a robust immunity against Trichinella infection.
  
  
  
  The full-length TsPmy coding DNA was cloned into the eukaryotic expression plasmid pVAX1, and the recombinant pVAX1/TsPmy was transformed into attenuated Salmonella typhimurium strain SL7207. Oral vaccination of mice with this attenuated Salmonella-delivered TsPmy DNA vaccine elicited a significant mucosal sIgA response in the intestine and a systemic IgG antibody response with IgG2a as the predominant subclass. Cytokine analysis also showed a significant increase in the Th1 (IFN-γ, IL-2) and Th2 (IL-4, 5, 6, 10) responses in lymphocytes from the spleen and MLNs of immunized mice upon stimulation with TsPmy protein. The expression of the homing receptors CCR9/CCR10 on antibody secreting B cells may be related to the translocation of IgA-secreted B cells to local intestinal mucosa. The mice immunized with Salmonella-delivered TsPmy DNA vaccine produced a significant 44.8% reduction in adult worm and a 46.6% reduction in muscle larvae after challenge with T. spiralis larvae.
  
  
  
  Our results demonstrated that oral vaccination with TsPmy DNA delivered by live attenuated S. typhimurium elicited a significant local IgA response and a mixed Th1/Th2 immune response that elicited a significant protection against T. spiralis infection in mice.
authors: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.|Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, PR China.
authors: Lei
authors: L
authors: Wang
authors: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
authors: Xiaohuan
authors: X
authors: Wang
authors: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
authors: Kuo
authors: K
authors: Bi
authors: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
authors: Ximeng
authors: X
authors: Sun
authors: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
authors: Jing
authors: J
authors: Yang
authors: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
authors: Yuan
authors: Y
authors: Gu
authors: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
authors: Jingjing
authors: J
authors: Huang
authors: Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas, United States of America.
authors: Bin
authors: B
authors: Zhan
authors: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing, PR China.
authors: Xinping
authors: X
authors: Zhu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D016207:Cytokines / Q000097:blood; D005260:Female; D005822:Genetic Vectors; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D054388:Receptors, CCR / Q000235:genetics; D054447:Receptors, CCR10 / Q000235:genetics; D011994:Recombinant Proteins / Q000276:immunology; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology; D058408:Th1-Th2 Balance; D017160:Trichinella spiralis / Q000302:isolation & purification; D014235:Trichinellosis / Q000276:immunology / Q000517:prevention & control*; D014335:Tropomyosin / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; C119309:CC chemokine receptor 9; D016207:Cytokines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D054388:Receptors, CCR; D054447:Receptors, CCR10; D011994:Recombinant Proteins; D014335:Tropomyosin; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA