title: Quantitative PCR Assays for Detecting Loach Minnow (Rhinichthys cobitis) and Spikedace (Meda fulgida) in the Southwestern United States.
abstract: Loach minnow (Rhinichthys cobitis) and spikedace (Meda fulgida) are legally protected with the status of Endangered under the U.S. Endangered Species Act and are endemic to the Gila River basin of Arizona and New Mexico. Efficient and sensitive methods for monitoring these species' distributions are critical for prioritizing conservation efforts. We developed quantitative PCR assays for detecting loach minnow and spikedace DNA in environmental samples. Each assay reliably detected low concentrations of target DNA without detection of non-target species, including other cyprinid fishes with which they co-occur.
authors: United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, MT, United States of America.
authors: Joseph C
authors: http://orcid.org/0000-0002-6790-7841
authors: JC
authors: Dysthe
authors: United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, MT, United States of America.
authors: Kellie J
authors: KJ
authors: Carim
authors: United States Department of Agriculture, Forest Service, Southwestern Region, Albuquerque, NM, United States of America.
authors: Yvette M
authors: YM
authors: Paroz
authors: United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, MT, United States of America.
authors: Kevin S
authors: KS
authors: McKelvey
authors: United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, MT, United States of America.
authors: Michael K
authors: MK
authors: Young
authors: United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, MT, United States of America.
authors: Michael K
authors: MK
authors: Schwartz
mesh_terms: D000818:Animals; D003247:Conservation of Natural Resources; D003530:Cyprinidae / Q000235:genetics*; D056727:Endangered Species; D016133:Polymerase Chain Reaction / Q000379:methods*; D015206:Southwestern United States