title: Pharmacokinetic study of ardisiacrispin A in rat plasma after intravenous administration by UPLC-MS/MS.
abstract: In this work, a sensitive and selective UPLC-MS/MS method for determination of ardisiacrispin A in rat plasma was developed. Cyasterone used as an internal standard (IS) and protein precipitation by acetonitrile-methanol (9:1, v/v) was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18column (2.1 × 100 mm, 1.7 μm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions m/z 1083.5 → 407.1 for ardisiacrispin A and m/z 521.3 → 485.2 for IS. Calibration plots were linear throughout the range 5-2000 ng/mL for ardisiacrispin A in rat plasma. Mean recoveries of ardisiacrispin A in rat plasma ranged from 80.4 to 92.6%. The values of RSD of intra- and inter-day precision were both <11%. The accuracy of the method was between 97.3 and 105.6%. The method was successfully applied to pharmacokinetic study of ardisiacrispin A after intravenous administration in rats.
authors: Department of Hematology, The People's Hospital of Lishui, Lishui, 323000, China.
authors: Bingmu
authors: B
authors: Fang
authors: The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, 325000, China.
authors: Shihui
authors: S
authors: Bao
authors: Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Lishui, 323000, China.
authors: Shuanghu
authors: S
authors: Wang
authors: Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Lishui, 323000, China.
authors: Minle
authors: M
authors: Chen
authors: Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China.
authors: Bingbao
authors: B
authors: Chen
authors: Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China.
authors: Ke
authors: K
authors: Su
authors: Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China.
authors: Congcong
authors: C
authors: Wen
authors: Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Lishui, 323000, China.
authors: Yunfang
authors: Y
authors: Zhou
authors: Analytical and Testing Center, Wenzhou Medical University, Wenzhou, 325035, China.
authors: Xianqin
authors: X
authors: Wang
authors: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
authors: Yuepeng
authors: Y
authors: Jin
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012503:Saponins / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D012503:Saponins; C054708:ardisiacrispin A; D009828:Oleanolic Acid
keywords: UPLC-MS/MS; ardisiacrispin A; pharmacokinetics; rat plasma