title: Effects of β-diketone antibiotics on F1-zebrafish (Danio rerio) based on high throughput miRNA sequencing under exposure to parents.
abstract: The toxicity of β-diketone antibiotics (DKAs), a class of ''pseudo-persistent'' environmental pollutants, to F0-zebrafish (Danio rerio) was investigated using 7-dpf F1-zebrafish miRNA sequencing and bioinformatics analyses. Based on relative expression, 47, 134 and 118 of 193 mature miRNAs were differentially expressed between control vs 6.25 mg/L, control vs 12.5 mg/L and 6.25 vs 12.5 mg/L treatments, respectively. Utilizing three databases, 2523 potential target genes were predicted, and they were assigned to 19 high-abundance KEGG pathways and 20 functional categories by COG analysis. Among 11 significantly differential expression and high-abundance miRNAs, the expression levels for 7 miRNAs (miR-144, -124, -499, -125b, -430b, -430c and -152) assessed by qRT-PCR were consistent with those determined by sRNA-seq. A potential network was plotted between 11 miRNAs and their target genes based on differential expression and binding effectiveness. The high degree of connectivity between miRNA-gene pairs suggests that these miRNAs play critical roles in zebrafish development. The expression of miR-124 and miR-499 in whole-mount in situ hybridization was in general agreement with those from qRT-PCR and miRNA-seq and were DKA concentration-dependent. DKA exposure induced severe histopathological changes and damage in F0-zebrafish ovary tissue, as reflected by an increased number of early developmental oocytes, irregular cell distribution, decreased yolk granules, cytoplasmic shrinkage, cell lysis in mature oocytes, and dissolution of internal corona radiata. Chronic DKA exposure affected reproduction of F0-zebrafish and development of F1-zebrafish. These observations demonstrate the toxic effect transfer relation across parent and their offspring, and enhance our understanding of drug-induced diseases.
authors: College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors: Yuansi
authors: Y
authors: Zheng
authors: College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors: Jiebo
authors: J
authors: Lin
authors: College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors: Jieyi
authors: J
authors: Li
authors: College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors: Haifeng
authors: H
authors: Zhang
authors: College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China.
authors: Weiming
authors: W
authors: Ai
authors: Key Laboratory of Watershed Sciences and Health of Zhejiang Province, Wenzhou Medical University, Wenzhou 325035, China.
authors: Xuedong
authors: X
authors: Wang
authors: Key Laboratory of Watershed Sciences and Health of Zhejiang Province, Wenzhou Medical University, Wenzhou 325035, China.
authors: Randy A
authors: RA
authors: Dahlgren
authors: College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: whuili@163.com.
authors: Huili
authors: H
authors: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000473:pathology; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics; D005260:Female; D024841:Fluoroquinolones / Q000633:toxicity*; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000235:genetics*; D013754:Tetracyclines / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D035683:MicroRNAs; D013754:Tetracyclines; D014874:Water Pollutants, Chemical
keywords: High throughput sequencing; Small RNA; Target genes; Zebrafish; miRNA; β-diketone antibiotics (DKAs)