title: Acute Ethanol Inhibition of γ Oscillations Is Mediated by Akt and GSK3β.
abstract: Hippocampal network oscillations at gamma band frequency (γ, 30-80 Hz) are closely associated with higher brain functions such as learning and memory. Acute ethanol exposure at intoxicating concentrations (≥50 mM) impairs cognitive function. This study aimed to determine the effects and the mechanisms of acute ethanol exposure on γ oscillations in an in vitro model. Ethanol (25-100 mM) suppressed kainate-induced γ oscillations in CA3 area of the rat hippocampal slices, in a concentration-dependent, reversible manner. The ethanol-induced suppression was reduced by the D1R antagonist SCH23390 or the PKA inhibitor H89, was prevented by the Akt inhibitor triciribine or the GSk3β inhibitor SB415286, was enhanced by the NMDA receptor antagonist D-AP5, but was not affected by the MAPK inhibitor U0126 or PI3K inhibitor wortmanin. Our results indicate that the intracellular kinases Akt and GSk3β play a critical role in the ethanol-induced suppression of γ oscillations and reveal new cellular pathways involved in the ethanol-induced cognitive impairment.
authors: Key Laboratory for the Brain Research of Henan Province, Xinxiang Medical UniversityXinxiang, China; Department of Pathophysiology, Xinxiang Medical UniversityXinxiang, China.
authors: JianGang
authors: J
authors: Wang
authors: Key Laboratory for the Brain Research of Henan Province, Xinxiang Medical UniversityXinxiang, China; Psychiatric Hospital of Henan ProvinceXinxiang, China.
authors: JingXi
authors: J
authors: Zhao
authors: Key Laboratory for the Brain Research of Henan Province, Xinxiang Medical UniversityXinxiang, China; Psychiatric Hospital of Henan ProvinceXinxiang, China.
authors: ZhiHua
authors: Z
authors: Liu
authors: Key Laboratory for the Brain Research of Henan Province, Xinxiang Medical UniversityXinxiang, China; Department of Neurobiology and Physiology, Xinxiang Medical UniversityXinxiang, China.
authors: FangLi
authors: F
authors: Guo
authors: Key Laboratory for the Brain Research of Henan Province, Xinxiang Medical UniversityXinxiang, China; Department of Neurobiology and Physiology, Xinxiang Medical UniversityXinxiang, China.
authors: Yali
authors: Y
authors: Wang
authors: Key Laboratory for the Brain Research of Henan Province, Xinxiang Medical University Xinxiang, China.
authors: Xiaofang
authors: X
authors: Wang
authors: Psychiatric Hospital of Henan Province Xinxiang, China.
authors: RuiLing
authors: R
authors: Zhang
authors: Department of Psychology, Xinxiang Medical UniversityHenan, China; Department of Health Sciences, Birmingham City UniversityBirmingham, UK.
authors: Martin
authors: M
authors: Vreugdenhil
authors: Key Laboratory for the Brain Research of Henan Province, Xinxiang Medical UniversityXinxiang, China; Psychiatric Hospital of Henan ProvinceXinxiang, China.
authors: Chengbiao
authors: C
authors: Lu
keywords: Akt; GSk3β; NMDA receptor; PKA; dopamine receptor; γ oscillation