title: Analysis of Extracellular Vesicles in the Tumor Microenvironment.
abstract: Extracellular vesicles (ECV) are membrane compartments shed from all types of cells in various physiological and pathological states. In recent years, ECV have gained an increasing interest from the scientific community for their role as an intercellular communicator that plays important roles in modifying the tumor microenvironment. Multiple techniques have been established to collect ECV from conditioned media of cell culture or physiological fluids. The gold standard methodology is differential centrifugation. Although alternative techniques exist to collect ECV, these techniques have not proven suitable as a substitution for the ultracentrifugation procedure.
authors: Division of Nephrology, Department of Medicine, McMaster University, Hamilton, ON, Canada. alnedaw@mcmaster.ca.|Hamilton Centre for Kidney Research (HCKR), St. Joseph's Hospital, Hamilton, ON, Canada. alnedaw@mcmaster.ca.
authors: Khalid
authors: K
authors: Al-Nedawi
authors: Division of Nephrology, Department of Medicine, McMaster University, Hamilton, ON, Canada.|Hamilton Centre for Kidney Research (HCKR), St. Joseph's Hospital, Hamilton, ON, Canada.
authors: Jolene
authors: J
authors: Read
mesh_terms: D001692:Biological Transport; D018929:Cell Culture Techniques; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D055252:Cell-Derived Microparticles / Q000378:metabolism; D055354:Exosomes; D000067128:Extracellular Vesicles / Q000378:metabolism*; D006801:Humans; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D059016:Tumor Microenvironment; D014461:Ultracentrifugation / Q000379:methods
keywords: Exosome; Microvesicle; Tumor microenvironment; Ultracentrifugation