title: Engineering levoglucosan metabolic pathway in Rhodococcus jostii RHA1 for lipid production.
abstract: Oleaginous strains of Rhodococcus including R. jostii RHA1 have attracted considerable attention due to their ability to accumulate triacylglycerols (TAGs), robust growth properties and genetic tractability. In this study, a novel metabolic pathway was introduced into R. jostii by heterogenous expression of the well-characterized gene, lgk encoding levoglucosan kinase from Lipomyces starkeyi YZ-215. This enables the recombinant R. jostii RHA1 to produce TAGs from the anhydrous sugar, levoglucosan, which can be generated efficiently as the major molecule from the pyrolysis of cellulose. The recombinant R. jostii RHA1 could grow on levoglucosan as the sole carbon source, and the consumption rate of levoglucosan was determined. Furthermore, expression of one more copy of lgk increased the enzymatic activity of LGK in the recombinant. However, the growth performance of the recombinant bearing two copies of lgk on levoglucosan was not improved. Although expression of lgk in the recombinants was not repressed by the glucose present in the media, glucose in the sugar mixture still affected consumption of levoglucosan. Under nitrogen limiting conditions, lipid produced from levoglucosan by the recombinant bearing lgk was up to 43.54 % of the cell dry weight, which was comparable to the content of lipid accumulated from glucose. This work demonstrated the technical feasibility of producing lipid from levoglucosan, an anhydrosugar derived from the pyrolysis of lignocellulosic materials, by the genetically modified rhodococci strains.
authors: Department of Biological Systems Engineering, Washington State University, L. J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA.
authors: Xiaochao
authors: X
authors: Xiong
authors: Department of Biological Systems Engineering, Washington State University, L. J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA.|Department of Chemical and Biological Engineering, Iowa State University, Ames, IA, 50011, USA.
authors: Jieni
authors: J
authors: Lian
authors: Department of Biological Systems Engineering, Washington State University, L. J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA.|Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
authors: Xiaochen
authors: X
authors: Yu
authors: Department of Biological Systems Engineering, Washington State University, L. J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA.
authors: Manuel
authors: M
authors: Garcia-Perez
authors: Department of Biological Systems Engineering, Washington State University, L. J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA. chens@wsu.edu.
authors: Shulin
authors: S
authors: Chen
mesh_terms: D002244:Carbon / Q000378:metabolism; D005947:Glucose / Q000031:analogs & derivatives* / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics; D012240:Rhodococcus / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D008055:Lipids; D014280:Triglycerides; C014989:1,6-anhydro-beta-glucopyranose; D002244:Carbon; D010770:Phosphotransferases; D005947:Glucose
keywords: Levoglucosan; Lignocellulosic biomass; Lipid; Pyrolysis oil; Rhodococcus jostii RHA1