title: Discerning the Chemistry in Individual Organelles with Small-Molecule Fluorescent Probes.
abstract: Principle has it that even the most advanced super-resolution microscope would be futile in providing biological insight into subcellular matrices without well-designed fluorescent tags/probes. Developments in biology have increasingly been boosted by advances of chemistry, with one prominent example being small-molecule fluorescent probes that not only allow cellular-level imaging, but also subcellular imaging. A majority, if not all, of the chemical/biological events take place inside cellular organelles, and researchers have been shifting their attention towards these substructures with the help of fluorescence techniques. This Review summarizes the existing fluorescent probes that target chemical/biological events within a single organelle. More importantly, organelle-anchoring strategies are described and emphasized to inspire the design of new generations of fluorescent probes, before concluding with future prospects on the possible further development of chemical biology.
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China.|Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore, Singapore, 117543, Singapore.|Laboratory of Bioimaging Probe Development, A*STAR, Singapore.|Department of Chemistry, Stanford University, USA.
authors: Wang
authors: W
authors: Xu
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China.
authors: Zebing
authors: Z
authors: Zeng
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China. jianhuijiang@hnu.edu.cn.
authors: Jian-Hui
authors: JH
authors: Jiang
authors: Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore, Singapore, 117543, Singapore. chmcyt@nus.edu.sg.|Laboratory of Bioimaging Probe Development, A*STAR, Singapore. chmcyt@nus.edu.sg.
authors: Young-Tae
authors: YT
authors: Chang
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China. lyuan@hnu.edu.cn.
authors: Lin
authors: 0000-0002-1015-5319
authors: L
authors: Yuan
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D015388:Organelles / Q000737:chemistry* / Q000378:metabolism; D054852:Small Molecule Libraries / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D054852:Small Molecule Libraries
keywords: anchoring motif; cell organelles; cellular analytes; fluorescence imaging; fluorescent probes