title: Seizure Suppression by High Temperature via cAMP Modulation in Drosophila.
abstract: Bang-sensitive (BS) Drosophila mutants display characteristic seizure-like activity (SLA) and paralysis after mechanical shock . After high-frequency electrical stimulation (HFS) of the brain, they generate robust seizures at very low threshold voltage. Here we report an important phenomenon, which effectively suppresses SLA in BS mutants. High temperature causes seizure suppression in all BS mutants (parabss1, eas, sda) examined in this study. This effect is fully reversible and flies show complete recovery from BS paralysis once the temperature effect is nullified. High temperature induces an increase in seizure threshold after a brief pulse of heat shock (HS). By genetic screening, we identified the involvement of cAMP in the suppression of seizures by high temperature. We propose that HS induces adenylyl cyclase which in turn increases cAMP concentration which eventually suppresses seizures in mutant flies. In summary, we describe an unusual phenomenon, where high temperature can suppress SLA in flies by modulating cAMP concentration.
authors: Department of Environmental Science, Policy and Management, University of California, Berkeley, California 94720 sarasarunesh@gmail.com.
authors: Arunesh
authors: A
authors: Saras
authors: Department of Environmental Science, Policy and Management, University of California, Berkeley, California 94720 Department of Molecular and Cell Biology, University of California Berkeley, California 94720.
authors: Mark A
authors: MA
authors: Tanouye
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal; D000242:Cyclic AMP; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D005838:Genotype; D006358:Hot Temperature; D009154:Mutation; D010641:Phenotype; D034622:RNA Interference; D012640:Seizures / Q000209:etiology* / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D000242:Cyclic AMP
keywords: FlyBook; cAMP; epilepsy; rutabaga; seizure suppression; sodium channel