title: Complex Class 1 Integron Carrying qnrB62 and blaVIM-2 in a Citrobacter freundii Clinical Isolate.
abstract: The coexistence of qnrB62 and blaVIM-2was detected in a Citrobacter clinical isolate. The reduced fluoroquinolone susceptibility is attributable to qnrB62, mutations of quinolone-resistance-determining regions, and an efflux pump or pumps. The genetic context surrounding chromosomal qnrB62 was a novel complex class 1 integron (In1184::ISCR1::qnrB62) containing a unique gene array (blaVIM-2-aacA4'-8-gucD). An 18-nucleotide deletion at the 3' end of the pspA gene [pspA(Δ18)], upstream of qnrB62, and an inverted repeat region (IRR2) were detected in In1184::ISCR1::qnrB62, indicating past transposition events.
authors: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea.
authors: Jae Jin
authors: JJ
authors: Lee
authors: Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea.
authors: Mi-Na
authors: MN
authors: Kim
authors: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea.
authors: Kwang Seung
authors: KS
authors: Park
authors: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea.
authors: Jung Hun
authors: JH
authors: Lee
authors: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea.
authors: Asad Mustafa
authors: AM
authors: Karim
authors: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea.|DNA Analysis Division, Seoul Institute, National Forensic Service, Yangcheongu, Seoul, Republic of Korea.
authors: Moonhee
authors: M
authors: Park
authors: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea.
authors: Ji Hwan
authors: JH
authors: Kim
authors: National Leading Research Laboratory of Drug Resistance Proteomics, Department of Biological Sciences, Myongji University, Yongin, Gyeonggido, Republic of Korea sangheelee@mju.ac.kr.
authors: Sang Hee
authors: SH
authors: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D016971:Citrobacter freundii / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D003227:Conjugation, Genetic; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics*; D024841:Fluoroquinolones / Q000494:pharmacology; D022761:Gene Transfer, Horizontal; D006801:Humans; D032023:Integrons / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009154:Mutation; D056910:Republic of Korea; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D024841:Fluoroquinolones; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases