title: Fluorescent carbon dot modified mesoporous silica nanocarriers for redox-responsive controlled drug delivery and bioimaging.
abstract: In this paper, a smart nanocarrier (MSNs-SS-CDPAA) is developed for redox-responsive controlled drug delivery and in vivo bioimaging by grafting fluorescent carbon dots to the surface of mesoporous silica nanoparticles (MSNs) via disulfide bonds. The polyanion polymer poly(acrylic acid) (PAA) was used to prepare the carboxyl-abundant carbon dots (CDPAA) by hydrothermal polymerization. The negatively charged CDPAA were anchored to the openings of MSNs containing the disulfide bonds through amidation and were used as gatekeepers for trapping the drugs within the pores. The in vitro release results indicated that the prepared MSNs-SS-CDPAA/DOX showed highly redox-responsive drug release in pH 7.4 and pH 5.0 PBS. In addition, the redox-responsive release mechanism was studied by measurement of the Zeta potential and fluorescence spectrophotometry. The prepared MSNs-SS-CDPAA exhibited excellent biocompatibility and fluorescence properties. Confocal laser scanning microscopy (CLSM) showed that MSNs-SS-CDPAA could emit blue, green and red fluorescence at an excitation wavelength of 408, 488 and 561nm, respectively. In addition, MSNs-SS-CDPAA/DOX exhibited a high cellular uptake as shown by CDPAA imaging and a therapeutic effect on cancer cells by MTT assay. This study describes a novel strategy for simultaneously controlled drug delivery and real-time imaging to track the behavior of nanoparticles during tumor therapy.
authors: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
authors: Jian
authors: J
authors: Jiao
authors: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
authors: Chang
authors: C
authors: Liu
authors: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
authors: Xian
authors: X
authors: Li
authors: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
authors: Jie
authors: J
authors: Liu
authors: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
authors: Donghua
authors: D
authors: Di
authors: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, PR China.
authors: Ying
authors: Y
authors: Zhang
authors: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China. Electronic address: zqf021110505@163.com.
authors: Qinfu
authors: Q
authors: Zhao
authors: Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China. Electronic address: silingwang@syphu.edu.cn.
authors: Siling
authors: S
authors: Wang
mesh_terms: D000072283:A549 Cells; D000180:Acrylic Resins / Q000737:chemistry; D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000494:pharmacology; D002244:Carbon / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D004220:Disulfides / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D004339:Drug Compounding; D016503:Drug Delivery Systems / Q000379:methods*; D065546:Drug Liberation; D055664:Electrochemical Techniques; D005456:Fluorescent Dyes / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D057054:Molecular Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D058105:Polymerization; D012822:Silicon Dioxide / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D000180:Acrylic Resins; D000903:Antibiotics, Antineoplastic; D004220:Disulfides; D005456:Fluorescent Dyes; C006903:carbopol 940; D002244:Carbon; D012822:Silicon Dioxide; D004317:Doxorubicin
keywords: Bioimaging; Carbon dots; Controlled drug delivery; Mesoporous silica nanoparticles; Redox-responsive