title: Photochemical Tagging for Quantitation of Unsaturated Fatty Acids by Mass Spectrometry.
abstract: Fatty acid (FA) profiling provides phenotypic information and is increasingly used in a broad range of biological and biomedical studies. Quantitation of unsaturated FAs with confident carbon-carbon double bond (C═C) location assignment is both sample and time consuming using traditional gas chromatography mass spectrometry analysis. In this study, we developed a rapid, sensitive, and quantitative method for profiling unsaturated FAs without using chromatographic separations. This method was based on a combination of in-solution photochemical tagging of a C═C in FAs and a subsequent gas-phase detagging via tandem (neutral loss scan) mass spectrometry. It enabled quantitation of unsaturated FAs from various biological samples (blood, plasma, and cell lines). More importantly, quantitative information on FA C═C location isomers, which was traditionally overlooked, could now be obtained and applied to studying FA changes between normal and cancerous human prostate cells.
authors: Department of Chemistry, Purdue University , West Lafayette, Indiana 47907, United States.|Weldon School of Biomedical Engineering, Purdue University , West Lafayette, Indiana 47907, United States.
authors: Xiaoxiao
authors: X
authors: Ma
authors: Department of Chemistry, Purdue University , West Lafayette, Indiana 47907, United States.
authors: Xu
authors: X
authors: Zhao
authors: Weldon School of Biomedical Engineering, Purdue University , West Lafayette, Indiana 47907, United States.
authors: Junjie
authors: J
authors: Li
authors: Weldon School of Biomedical Engineering, Purdue University , West Lafayette, Indiana 47907, United States.
authors: Wenpeng
authors: W
authors: Zhang
authors: Weldon School of Biomedical Engineering, Purdue University , West Lafayette, Indiana 47907, United States.|Purdue University Center for Cancer Research, Purdue University , West Lafayette, Indiana 47907, United States.
authors: Ji-Xin
authors: JX
authors: Cheng
authors: Weldon School of Biomedical Engineering, Purdue University , West Lafayette, Indiana 47907, United States.
authors: Zheng
authors: Z
authors: Ouyang
authors: Department of Chemistry, Purdue University , West Lafayette, Indiana 47907, United States.
authors: Yu
authors: Y
authors: Xia
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D005231:Fatty Acids, Unsaturated / Q000032:analysis* / Q000097:blood*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005231:Fatty Acids, Unsaturated