title: Enhanced electrochemical detection of quercetin by Natural Deep Eutectic Solvents.
abstract: New trends in analytical chemistry encourage the development of smart techniques and methods aligned with Green Chemistry. In this sense, Natural Deep Eutectic Solvents represents an excellent opportunity as a new generation of green solvents. In this work a new application for them has been proposed and demonstrated. These solvents were synthesized by combinations of inexpensive and natural components like, Glucose, Fructose, Citric acid and Lactic acid. The different natural solvents were easily prepared and added to buffer solution in different concentrations, allowing the enhancement of electrochemical detection of an important representative antioxidant like quercetin (QR) with improved signal up to 380%. QR is a ubiquitous flavonoid widespread in plants and food of plant origin. The proposed method using phosphate buffer with a eutectic mixture of Citric acid, Glucose and water in combination with carbon screen printed electrodes exhibited a good analytical performance. Detection and quantification limits were of 7.97 and 26.3 nM respectively; and repeatability with %RSDs of 1.41 and 7.49 for peak potential and intensity respectively. In addition, it has proved to be faster, greener and cheaper than other sensors and chromatographic methods available with the additional advantage of being completely portable. Furthermore, the obtained results demonstrated that the proposed method is able for the determination of QR in complex food samples.
authors: Instituto de Química San Luis (INQUISAL- CONICET), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina.
authors: Federico José Vicente
authors: FJ
authors: Gomez
authors: Instituto de Biología Agrícola de Mendoza (IBAM-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina.
authors: Magdalena
authors: M
authors: Espino
authors: Instituto de Biología Agrícola de Mendoza (IBAM-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina.
authors: María
authors: M
authors: de Los Angeles Fernandez
authors: Instituto de Química San Luis (INQUISAL- CONICET), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina.
authors: Julio
authors: J
authors: Raba
authors: Instituto de Biología Agrícola de Mendoza (IBAM-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina. Electronic address: msilva@fca.uncu.edu.ar.
authors: María Fernanda
authors: MF
authors: Silva
mesh_terms: D001688:Biological Products / Q000138:chemical synthesis / Q000737:chemistry*; D055664:Electrochemical Techniques; D011794:Quercetin / Q000032:analysis*; D012997:Solvents / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D001688:Biological Products; D012997:Solvents; D011794:Quercetin
keywords: Enhanced electrochemical detection; NADES; Onions; Polyphenols; Quercetin