title: Effect of lithium chloride on cell proliferation and osteogenic differentiation in stem cells from human exfoliated deciduous teeth.
abstract: Lithium Chloride (LiCl) has been used as a canonical Wnt pathway activator due to its ability to inhibit a glycogen synthase kinase-3. The aim of the present study was to investigate the effect of LiCl on cell proliferation and osteogenic differentiation in stem cells isolated from human exfoliated deciduous teeth (SHEDs). SHEDs were isolated and cultured in media supplemented with LiCl at 5, 10, or 20mM. The results demonstrated that LiCl significantly decreased SHEDs colony forming unit ability in a dose dependent manner. LiCl significantly enhanced the percentage of cells in the sub G0 phase, accompanied by a reduction of the percentage of cells in the G1 phase at day 3 and 7 after treatment. Further, LiCl markedly decreased OSX and DMP1 mRNA expression after treating SHEDs in an osteogenic induction medium for 7 days. In addition, no significant difference in alkaline phosphatase enzymatic activity or mineral deposition was found. Together, these results imply that LiCl influences SHEDs behavior.
authors: Department of Pediatric Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand; Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand.
authors: Panarat
authors: P
authors: Rattanawarawipa
authors: Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand; Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand.
authors: Prasit
authors: P
authors: Pavasant
authors: Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand; Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand.
authors: Thanaphum
authors: T
authors: Osathanon
authors: Department of Pediatric Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand; Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330 Thailand. Electronic address: wsukarawan@hotmail.com.
authors: Waleerat
authors: W
authors: Sukarawan
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D003782:Dental Pulp / Q000187:drug effects* / Q000254:growth & development; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors; D006801:Humans; D018021:Lithium Chloride / Q000008:administration & dosage*; D010012:Osteogenesis / Q000187:drug effects*; D010750:Phosphoproteins / Q000096:biosynthesis; D000076182:Sp7 Transcription Factor; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D014094:Tooth, Deciduous / Q000187:drug effects / Q000254:growth & development; D014157:Transcription Factors / Q000096:biosynthesis; D060449:Wnt Signaling Pathway / Q000187:drug effects
chemical_list: C513309:DMP1 protein, human; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D000076182:Sp7 Transcription Factor; C478163:SP7 protein, human; D014157:Transcription Factors; D038362:Glycogen Synthase Kinase 3; C448021:glycogen synthase kinase 3 alpha; D018021:Lithium Chloride
keywords: Differentiation; Lithium chloride; Osteogenic; Proliferation; Stem cells