title: Osteogenic activity and antibacterial effect of zinc oxide/carboxylated graphene oxide nanocomposites: Preparation and in vitro evaluation.
abstract: The aim of this study was to prepare nanocomposites of carboxylated graphene oxide (GO-COOH) sheets decorated with zinc oxide (ZnO) nanoparticles (NPs) and investigate their advantages in the field of bone tissue engineering. First, ZnO/GO-COOH nanocomposites were synthesized by facile reactions, including the carboxylation of graphene oxide (GO) and the nucleation of ZnO on GO-COOH sheets. The synthesized ZnO/GO-COOH nanocomposites were then characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectra, and transmission electron microscopy (TEM). The biocompatibility, osteogenic activity and antibacterial effect of ZnO/GO-COOH nanocomposites were further investigated. In the nanocomposites, ZnO nanoparticles with a size of approximately 12nm were uniformly decorated on GO-COOH sheets. Compared with GO-COOH and the control group, ZnO/GO-COOH nanocomposites significantly enhanced ALP activity, osteocalcin production and extracellular matrix mineralization as well as up-regulated osteogenic-related genes (ALP, OCN, and Runx2) in MG63 osteoblast-like cells. Moreover, ZnO/GO-COOH nanocomposites had an antibacterial effect against Streptococcus mutans. These results indicated that ZnO/GO-COOH nanocomposites exhibited both osteogenic activity and antibacterial effect and had great potential for designing new biomaterials in the field of bone tissue engineering.
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Junyu
authors: J
authors: Chen
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Xin
authors: X
authors: Zhang
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: He
authors: H
authors: Cai
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Zhiqiang
authors: Z
authors: Chen
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Tong
authors: T
authors: Wang
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Lingling
authors: L
authors: Jia
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Jian
authors: J
authors: Wang
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. Electronic address: champion@scu.edu.cn.
authors: Qianbing
authors: Q
authors: Wan
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. Electronic address: xbpei@hotmail.com.
authors: Xibo
authors: X
authors: Pei
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000008:administration & dosage* / Q000737:chemistry; D015675:Osteocalcin / Q000378:metabolism; D012516:Osteosarcoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D056951:Photoelectron Spectroscopy; D060888:Real-Time Polymerase Chain Reaction; D013295:Streptococcus mutans / Q000187:drug effects*; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D015675:Osteocalcin; D006108:Graphite; D000469:Alkaline Phosphatase; D015034:Zinc Oxide
keywords: Antibacterial effect; Carboxylated graphene oxide; Nanocomposites; Osteogenic activity; Zinc oxide