title: Characterization of pediatric microtia cartilage: a reservoir of chondrocytes for auricular reconstruction using tissue engineering strategies.
abstract: The external ear is composed of elastic cartilage. Microtia is a congenital malformation of the external ear that involves a small reduction in size or a complete absence. The aim of tissue engineering is to regenerate tissues and organs clinically implantable based on the utilization of cells and biomaterials. Remnants from microtia represent a source of cells for auricular reconstruction using tissue engineering. To examine the macromolecular architecture of microtia cartilage and behavior of chondrocytes, in order to enrich the knowledge of this type of cartilage as a cell reservoir. Auricular cartilage remnants were obtained from pediatric patients with microtia undergoing reconstructive procedures. Extracellular matrix composition was characterized using immunofluorescence and histological staining methods. Chondrocytes were isolated and expanded in vitro using a mechanical-enzymatic protocol. Chondrocyte phenotype was analyzed using qualitative PCR. Microtia cartilage preserves structural organization similar to healthy elastic cartilage. Extracellular matrix is composed of typical cartilage proteins such as type II collagen, elastin and proteoglycans. Chondrocytes displayed morphological features similar to chondrocytes derived from healthy cartilage, expressing SOX9, COL2 and ELN, thus preserving chondral phenotype. Cell viability was 94.6 % during in vitro expansion. Elastic cartilage from microtia has similar characteristics, both architectural and biochemical to healthy cartilage. We confirmed the suitability of microtia remnant as a reservoir of chondrocytes with potential to be expanded in vitro, maintaining phenotypical features and viability. Microtia remnants are an accessible source of autologous cells for auricular reconstruction using tissue engineering strategies.
authors: Laboratorio de Biotecnología, Centro Nacional de Investigación y Atención de Quemados (CENIAQ), Instituto Nacional de Rehabilitación, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico.
authors: Y
authors: Y
authors: Melgarejo-Ramírez
authors: Laboratorio de Biotecnología, Centro Nacional de Investigación y Atención de Quemados (CENIAQ), Instituto Nacional de Rehabilitación, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico.
authors: R
authors: R
authors: Sánchez-Sánchez
authors: Unidad de Ingeniería de tejidos, terapia celular y medicina regenerativa, Instituto Nacional de Rehabilitación, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, D.F., Mexico.
authors: J
authors: J
authors: García-López
authors: Laboratorio de Biotecnología, Centro Nacional de Investigación y Atención de Quemados (CENIAQ), Instituto Nacional de Rehabilitación, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico.
authors: A M
authors: AM
authors: Brena-Molina
authors: División de cirugía plástica y reconstructiva, Hospital General Dr. Manuel Gea González, Calz. De Tlalpan No. 4800 Col. Sección XVI, C.P. 14080, Mexico City, Mexico.
authors: C
authors: C
authors: Gutiérrez-Gómez
authors: Unidad de Ingeniería de tejidos, terapia celular y medicina regenerativa, Instituto Nacional de Rehabilitación, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, D.F., Mexico.
authors: C
authors: C
authors: Ibarra
authors: Laboratorio de Biotecnología, Centro Nacional de Investigación y Atención de Quemados (CENIAQ), Instituto Nacional de Rehabilitación, Calzada México-Xochimilco No. 289, Col. Arenal de Guadalupe, C.P. 14389, Mexico City, Mexico. mvelasquillo@gmail.com.
authors: C
authors: C
authors: Velasquillo
mesh_terms: D049109:Cell Proliferation; D002469:Cell Separation; D048430:Cell Shape; D002648:Child; D002675:Child, Preschool; D019902:Chondrocytes / Q000473:pathology*; D024043:Collagen Type II / Q000378:metabolism; D065817:Congenital Microtia / Q000473:pathology*; D004425:Ear Cartilage / Q000473:pathology*; D004549:Elastin / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D010641:Phenotype; D019651:Plastic Surgery Procedures / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D024043:Collagen Type II; D004549:Elastin
keywords: Auricular reconstruction; Auricular remnant; Elastic cartilage; Microtia; Tissue engineering