title: Self-assembled cyclodextrin-modified gold nanoparticles on silica beads as stationary phase for chiral liquid chromatography and hydrophilic interaction chromatography.
abstract: A facile strategy based on self-assembly of Au nanoparticles (AuNPs) (60±10nm in size) on the surfaces of amino-functionalized porous silica spheres under mild conditions was proposed. The resulting material possessed a core-shell structure in which AuNPs were the shell and silica spheres were the core. Then, thiolated-β-cyclodextrin (SH-β-CD) was covalently attached onto the AuNPs as chiral selector for the enantioseparation. The resultant packing material was evaluated by high-performance liquid chromatography (HPLC). The separations of nine pairs of enantiomers were achieved by using the new chiral stationary phase (CSP) in the reversed-phase liquid chromatography (RPLC) mode, respectively. The results showed the new CSP have more sufficient interaction with the analytes due to the existence of AuNPs on silica surfaces, resulting in faster mass transfer rate, compared with β-CD modified silica column. The result shed light on potential usage of chemical modified NPs as chiral selector for enantioseparation based on HPLC. In addition, the new phase was also used in hydrophilic interaction liquid chromatography (HILIC) to separate polar compounds and highly hydrophilic compounds.
authors: Key Laboratory of Energy and Chemical Engineering, Ningxia University, Yinchuan 750021, China; Institute of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China. Electronic address: liyuanyuan_abc@sohu.com.
authors: Yuanyuan
authors: Y
authors: Li
authors: Key Laboratory of Energy and Chemical Engineering, Ningxia University, Yinchuan 750021, China; Institute of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China.
authors: Manman
authors: M
authors: Wei
authors: Zhenjiang Entry-exit Inspection and Quarantine Bureau, Zhenjiang 212008, China.
authors: Tong
authors: T
authors: Chen
authors: Key Laboratory of Energy and Chemical Engineering, Ningxia University, Yinchuan 750021, China; Institute of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China.
authors: Nan
authors: N
authors: Zhu
authors: Key Laboratory of Energy and Chemical Engineering, Ningxia University, Yinchuan 750021, China; Institute of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China.
authors: Yulong
authors: Y
authors: Ma
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D006046:Gold / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D053768:Metal Nanoparticles / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry; D010575:Pesticides / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D009705:Nucleosides; D010575:Pesticides; D047392:beta-Cyclodextrins; D006046:Gold; D012822:Silicon Dioxide
keywords: Au nanoparticles; Chiral separation; Hydrophilic interaction liquid chromatography; SiO(2)@Au core-shell structure; β-cyclodextrin