title: Targeted systemic mesenchymal stem cell delivery using hyaluronate - wheat germ agglutinin conjugate.
abstract: A variety of receptors for hyaluronate (HA), a natural linear polysaccharide, were found in the body, which have been exploited as target sites for HA-based drug delivery systems. In this work, mesenchymal stem cells (MSCs) were surface-modified with HA - wheat germ agglutinin (WGA) conjugate for targeted systemic delivery of MSCs to the liver. WGA was conjugated to HA by coupling reaction between aldehyde-modified HA and amine group of WGA. The conjugation of WGA to HA was corroborated by gel permeation chromatography (GPC) and the successful surface modification of MSCs with HA-WGA conjugate was confirmed by confocal microscopy. The synthesized HA-WGA conjugate could be incorporated onto the cellular membrane by agglutinating the cell-associated carbohydrates. Fluorescent imaging for in vivo biodistribution visualized the targeted delivery of the HA-WGA/MSC complex to the liver after intravenous injection. This new strategy for targeted delivery of MSCs using HA-WGA conjugate might be successfully exploited for various regenerative medicines including cell therapy.
authors: Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Korea.
authors: Yun Seop
authors: YS
authors: Kim
authors: Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Korea.
authors: Won Ho
authors: WH
authors: Kong
authors: Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Korea.
authors: Hyemin
authors: H
authors: Kim
authors: Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Korea. Electronic address: skhanb@postech.ac.kr.
authors: Sei Kwang
authors: SK
authors: Hahn
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006820:Hyaluronic Acid / Q000737:chemistry / Q000493:pharmacokinetics*; D008099:Liver / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D008115:Liver Regeneration / Q000502:physiology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D058726:Nanoconjugates / Q000737:chemistry* / Q000648:ultrastructure; D009928:Organ Specificity / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D014909:Wheat Germ Agglutinins / Q000737:chemistry*
chemical_list: D053769:Nanocapsules; D058726:Nanoconjugates; D014909:Wheat Germ Agglutinins; D006820:Hyaluronic Acid
keywords: Cell surface modification; Hyaluronate; Mesenchymal stem cell; Targeted delivery; Wheat germ agglutinin