title: Preparation and inhibition on α-d-glucosidase of low molecular weight polysaccharide from Cordyceps militaris.
abstract: The structural properties and the inhibition on α-d-glucosidase activity of the low molecular weight (LCMPs-II) obtained from the optimized acid hydrolysis of the Cordyceps militaris polysaccharides (CMPs) were investigated in this paper. The LCMPs-II with a molecular weight of 28 KDa mainly composed of rhamnose, xylose and glucose with the molar ratio of 1: 2.19: 6.73 was separated from LCMPs-I which was the acid hydrolysis product of CMPs by chromatography on Sephadex G-100 column. The solubility of LCMPs-II was tested to be 32.12±1.05g in 100mL distilled water under 25°C. Its solubility was almost as twice as that of CMPs. Afterward, the structural features of LCMPs-II was investigated by a combination of chemical and instrumental analysis such as the specific rotation determination, FT-IR, periodate oxidation-Smith degradation, Congo-red, GC, scanning electron microscope and NMR. The results showed that the optical rotation of LCMPs-II was +25° and it was 1,3-branched-rhamnoxyloglucan which had a linear backbone of (1→4)-linked α-d-glucopyranose (α-d-Glcp units).
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economic and Technological Development Area, Tianjin 300457, PR China. Electronic address: zhyuanzhu@tust.edu.cn.
authors: Zhen-Yuan
authors: ZY
authors: Zhu
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economic and Technological Development Area, Tianjin 300457, PR China.
authors: Ming-Zhu
authors: MZ
authors: Guo
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economic and Technological Development Area, Tianjin 300457, PR China.
authors: Fei
authors: F
authors: Liu
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economic and Technological Development Area, Tianjin 300457, PR China.
authors: You
authors: Y
authors: Luo
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economic and Technological Development Area, Tianjin 300457, PR China.
authors: Lu
authors: L
authors: Chen
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economic and Technological Development Area, Tianjin 300457, PR China.
authors: Meng
authors: M
authors: Meng
authors: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economic and Technological Development Area, Tianjin 300457, PR China.
authors: Xiao-Ting
authors: XT
authors: Wang
authors: Université Pierre et Marie Curie-Paris 6, Institut Parisien de Chimie Moléculaire, CNR UMR 8232, 4 place Jussieu, 75005, Paris, France.
authors: Yong-Min
authors: YM
authors: Zhang
mesh_terms: D032661:Cordyceps / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000648:ultrastructure; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry*; D006868:Hydrolysis; D008970:Molecular Weight; D012995:Solubility; D000520:alpha-Glucosidases / Q000737:chemistry*
chemical_list: D062610:Fungal Polysaccharides; D065089:Glycoside Hydrolase Inhibitors; D000520:alpha-Glucosidases
keywords: Acid hydrolysis; Cordyceps militaris; Inhibitory activity