title: Pancreas-enriched miRNAs are altered in the circulation of subjects with diabetes: a pilot cross-sectional study.
abstract: The clinical presentation of diabetes sometimes overlaps, contributing to ambiguity in the diagnosis. Thus, circulating pancreatic islet-enriched microRNAs (miRNAs) might be useful biomarkers of β-cell injury/dysfunction that would allow more accurate subtyping of diabetes. We measured plasma levels of selected miRNAs in subjects with prediabetes (n = 12), type 2 diabetes (T2D, n = 31), latent autoimmune diabetes of adults (LADA, n = 6) and type 1 diabetes (T1D, n = 16) and compared them to levels in healthy control subjects (n = 27). The study was conducted at the Translational Research Institute for Metabolism and Diabetes (TRI-MD), Florida Hospital. MiRNAs including miR-375 (linked to β-cell injury), miR-21 (associated with islet inflammation), miR-24.1, miR-30d, miR-34a, miR-126, miR-146, and miR-148a were significantly elevated in subjects with various forms of diabetes compared to healthy controls. Levels of several miRNAs were significantly correlated with glucose responses during oral glucose tolerance testing, HbA1c, β-cell function, and insulin resistance in healthy controls, prediabetes, and T2D. These data suggest that miRNAs linked to β-cell injury and islet inflammation might be useful biomarkers to distinguish between subtypes of diabetes. This information could be used to predict progression of the disease, guide selection of optimal therapy and monitor responses to interventions, thus improving outcomes in patients with diabetes.
authors: Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Orlando, FL, USA.|MIT Research Affiliate, Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.|Sanford Burnham Medical Research Institute, Orlando, FL, USA.
authors: Attila A
authors: AA
authors: Seyhan
authors: Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Orlando, FL, USA.
authors: Yury O
authors: YO
authors: Nunez Lopez
authors: Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Orlando, FL, USA.
authors: Hui
authors: H
authors: Xie
authors: Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Orlando, FL, USA.
authors: Fanchao
authors: F
authors: Yi
authors: Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, Gainesville, FL, USA.
authors: Clayton
authors: C
authors: Mathews
authors: College of Medicine Hospital, University of Central Florida, Orlando, FL, USA.
authors: Magdalena
authors: M
authors: Pasarica
authors: Translational Research Institute for Metabolism and Diabetes, Florida Hospital, Orlando, FL, USA.|Sanford Burnham Medical Research Institute, Orlando, FL, USA.
authors: Richard E
authors: RE
authors: Pratley
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D003430:Cross-Sectional Studies; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D005260:Female; D005431:Florida; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D010179:Pancreas / Q000378:metabolism*; D010865:Pilot Projects; D011236:Prediabetic State / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D035683:MicroRNAs