title: Allicin protects traumatic spinal cord injury through regulating the HSP70/Akt/iNOS pathway in mice.
abstract: Allicin is a major component of garlic, extracted as an oily liquid. The present study was designed to investigate the beneficial effects of allicin on traumatic spinal cord injury (TSCI) in mice, and whether the effects are mediated via regulation of the heat shock protein 70 (HSP70), v‑akt murine thymoma viral oncogene homolog 1 (Akt) and inducible nitric oxide synthase (iNOS) pathways. Adult BALB/c mice (30‑40 g) received a laminectomy at the T9 vertebral level as a model of TSCI. In the present study, treatment of the TSCI mice with allicin significantly increased their Basso, Beattie and Bresnahan (BBB) scores (P<0.01) and reduced the spinal cord water content (P<0.01). This protective effect was associated with the inhibition of oxidative stress and inflammatory responses in TSCI mice. Western blot analysis demonstrated that allicin increased the protein levels of HSP70, increased the phosphorylation of Akt and reduced the iNOS protein expression levels in TSCI mice. Additionally, treatment with allicin significantly reduced the levels of ROS and enhanced the NADH levels in TSCI mice. Collectively, these data demonstrate that the effects of allicin on TSCI are mediated via regulation of the HSP70, Akt and iNOS pathways in mice.
authors: Department of Orthopedics, The First Central Hospital of Baoding, Baoding, Hebei 071000, P.R. China.
authors: Shunyi
authors: S
authors: Wang
authors: Department of Orthopedics, The First Central Hospital of Baoding, Baoding, Hebei 071000, P.R. China.
authors: Dongliang
authors: D
authors: Ren
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000627:therapeutic use*; D004220:Disulfides; D005737:Garlic / Q000737:chemistry; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018696:Neuroprotective Agents / Q000627:therapeutic use; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D020127:Recovery of Function; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D013441:Sulfinic Acids / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D004220:Disulfides; D018840:HSP70 Heat-Shock Proteins; D018696:Neuroprotective Agents; D013441:Sulfinic Acids; C006452:allicin; D052247:Nitric Oxide Synthase Type II; D051057:Proto-Oncogene Proteins c-akt