title: Physiological basis for low-temperature survival and storage of quiescent larvae of the fruit fly Drosophila melanogaster.
abstract: The cryopreservation techniques proposed for embryos of the fruit fly Drosophila melanogaster are not yet ready for practical use. Alternative methods for long-term storage of D. melanogaster strains, although urgently needed, do not exist. Herein, we describe a narrow interval of low temperatures under which the larvae of D. melanogaster can be stored in quiescence for up to two months. The development of larvae was arrested at the pre-wandering stage under fluctuating thermal regime (FTR), which simultaneously resulted in diminishing the accumulation of indirect chill injuries. Our physiological, metabolomic, and transcriptomic analyses revealed that compared to larvae stored at constant low temperatures, the larvae stored under FTR conditions were able to decrease the rates of depletion of energy substrates, exploited brief warm episodes of FTR for homeostatic control of metabolite levels, and more efficiently exerted protection against oxidative damage.
authors: Institute of Entomology, Biology Centre CAS, Branišovská 31, 37005 České Budějovice, Czech Republic.
authors: Vladimír
authors: V
authors: Koštál
authors: Institute of Entomology, Biology Centre CAS, Branišovská 31, 37005 České Budějovice, Czech Republic.
authors: Jaroslava
authors: J
authors: Korbelová
authors: Institute of Entomology, Biology Centre CAS, Branišovská 31, 37005 České Budějovice, Czech Republic.|Faculty of Science, University of South Bohemia, Branišovská 31, 37005 České Budějovice, Czech Republic.
authors: Tomáš
authors: T
authors: Štětina
authors: Institute of Entomology, Biology Centre CAS, Branišovská 31, 37005 České Budějovice, Czech Republic.|Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria.
authors: Rodolphe
authors: R
authors: Poupardin
authors: Université de Rennes 1, UMR CNRS 6553 ECOBIO, 263 Avenue du Général-Leclerc, 35042 Rennes, France.
authors: Hervé
authors: H
authors: Colinet
authors: Institute of Entomology, Biology Centre CAS, Branišovská 31, 37005 České Budějovice, Czech Republic.
authors: Helena
authors: H
authors: Zahradníčková
authors: Institute of Entomology, Biology Centre CAS, Branišovská 31, 37005 České Budějovice, Czech Republic.
authors: Iva
authors: I
authors: Opekarová
authors: Institute of Entomology, Biology Centre CAS, Branišovská 31, 37005 České Budějovice, Czech Republic.
authors: Martin
authors: M
authors: Moos
authors: Institute of Entomology, Biology Centre CAS, Branišovská 31, 37005 České Budějovice, Czech Republic.
authors: Petr
authors: P
authors: Šimek
mesh_terms: D000818:Animals; D003080:Cold Temperature / Q000009:adverse effects*; D000073920:Cryobiology; D015925:Cryopreservation; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D007814:Larva / Q000235:genetics / Q000502:physiology*