title: Delivery of the Porcupine Inhibitor WNT974 in Mice.
abstract: We describe here a technique for delivering the porcupine inhibitor WNT974 (formerly LGK974) in mice. The protocol entails once-a-day oral delivery of WNT974 for up to 3 months at a concentration sufficient to achieve systemic Wnt pathway inhibition with limited toxicity as measured by weight change. This route of delivery enables extended durations of Wnt signaling inhibition in a mammalian model organism.
authors: Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390-9039, USA.
authors: Li-Shu
authors: LS
authors: Zhang
authors: Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390-9039, USA.
authors: Lawrence
authors: L
authors: Lum
mesh_terms: D000217:Acyltransferases / Q000037:antagonists & inhibitors* / Q000235:genetics; D000818:Animals; D008565:Membrane Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D051379:Mice; D008967:Molecular Biology / Q000379:methods*; D011719:Pyrazines / Q000008:administration & dosage; D011725:Pyridines / Q000008:administration & dosage; D051153:Wnt Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D060449:Wnt Signaling Pathway / Q000187:drug effects*
chemical_list: D008565:Membrane Proteins; D011719:Pyrazines; D011725:Pyridines; D051153:Wnt Proteins; D000217:Acyltransferases; C491187:Porcn protein, mouse; C586458:LGK974
keywords: Drug delivery; Mice; Porcupine; Wnt974/LGK974