title: miRNome Analysis of CML Cells.
abstract: Next-generation sequencing technologies have greatly accelerated the biological and medical progression. As one of the applications, miRNA-Seq is invaluable in detecting and characterizing genome-wide miRNAs of either too high or too low abundance. Besides, it can also be used in detecting novel miRNAs. Here, we describe an ab initio analysis of an example chronic myeloid leukemia miRNA sequencing data set to quantify the global expression of miRNAs, detect differential expression and novel miRNAs, and predict target genes. The run time of this protocol may vary depending on the volume of miRNA sequencing data and available computing resources but takes ~5 h of computing time for typical experiments and less than 1 h of hands-on time.
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.1-104 Beichen West Road, Chaoyang District, Beijing, 100101, China.
authors: Yadong
authors: Y
authors: Yang
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.1-104 Beichen West Road, Chaoyang District, Beijing, 100101, China.
authors: Nan
authors: N
authors: Ding
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.1-104 Beichen West Road, Chaoyang District, Beijing, 100101, China.
authors: Xunong
authors: X
authors: Dong
authors: CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.1-104 Beichen West Road, Chaoyang District, Beijing, 100101, China. fangxd@big.ac.cn.
authors: Xiangdong
authors: X
authors: Fang
mesh_terms: D019295:Computational Biology / Q000379:methods; D015973:Gene Expression Regulation, Leukemic; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*; D012984:Software
chemical_list: D035683:MicroRNAs
keywords: Differential expression; Sequencing; Target; miRNome