title: [Effect of transcription activity regulated by VNTR-ZNF and -14C/T variants in the promoter region of ATP-binding cassette transporter 1 in HepG2 cells].
abstract: OBJECTIVE
  To explore the effect of VNTR-ZNF and -14C/T variants of the promoter region of the ABCA1 gene on the transcription activity of genes in vitro.
  
  
  METHODS
  The recombinants were constructed by ligating DNA fragment containing VNTR-ZNF ACCCC inserted/deleted allele with or without -14C/T substitution fragments with a PGL2-basic vector containing luciferase reporter gene. The recombinants were then transfected into HepG2 cells using the cationic lipid method. After 48 h, transfected cells were collected and used to detect the luciferase activity.
  
  
  RESULTS
  Luciferase activity of PGL2-ZNF-ACCCCDel was greater than that of PGL2-ZNF-ACCCCIns. Luciferase activity of PGL2-ZNFDel-14C was greater than that of PGL2-ZNFDel-14T, PGL2-ZNFIns-14C, PGL2-ZNFIns-14T.
  
  
  CONCLUSIONS
  Compared with the insertion type, the ACCCC-deleted type of VNTR-ZNF can significantly enhance the transcription activity of ABCA1. And co-transfection of -14 C allele can further enhance this activity.
authors: Department of Laboratory Medicine, Tianjin Chest Hospital, Tianjin 300222, China; Institute of Cardiovascular Disease, Tianjin 300222, China. Email: yongminmao@163.com.
authors: Shenxia
authors: S
authors: Gao
authors: Lili
authors: L
authors: Zhao
authors: Ying
authors: Y
authors: Zhang
authors: Yongmin
authors: Y
authors: Mao
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000235:genetics*; D001483:Base Sequence; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D018598:Minisatellite Repeats / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D064286:ATP Binding Cassette Transporter 1; D008156:Luciferases