title: Tma108, a putative M1 aminopeptidase, is a specific nascent chain-associated protein in Saccharomyces cerevisiae.
abstract: The discovery of novel specific ribosome-associated factors challenges the assumption that translation relies on standardized molecular machinery. In this work, we demonstrate that Tma108, an uncharacterized translation machinery-associated factor in yeast, defines a subpopulation of cellular ribosomes specifically involved in the translation of less than 200 mRNAs encoding proteins with ATP or Zinc binding domains. Using ribonucleoparticle dissociation experiments we established that Tma108 directly interacts with the nascent protein chain. Additionally, we have shown that translation of the first 35 amino acids of Asn1, one of the Tma108 targets, is necessary and sufficient to recruit Tma108, suggesting that it is loaded early during translation. Comparative genomic analyses, molecular modeling and directed mutagenesis point to Tma108 as an original M1 metallopeptidase, which uses its putative catalytic peptide-binding pocket to bind the N-terminus of its targets. The involvement of Tma108 in co-translational regulation is attested by a drastic change in the subcellular localization of ATP2 mRNA upon Tma108 inactivation. Tma108 is a unique example of a nascent chain-associated factor with high selectivity and its study illustrates the existence of other specific translation-associated factors besides RNA binding proteins.
authors: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie computationnelle et quantitative - Institut de Biologie Paris Seine (LCQB - IBPS), 75005 Paris, France.
authors: Thierry
authors: T
authors: Delaveau
authors: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie computationnelle et quantitative - Institut de Biologie Paris Seine (LCQB - IBPS), 75005 Paris, France.
authors: Dimitri
authors: D
authors: Davoine
authors: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie computationnelle et quantitative - Institut de Biologie Paris Seine (LCQB - IBPS), 75005 Paris, France.
authors: Ariane
authors: A
authors: Jolly
authors: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie computationnelle et quantitative - Institut de Biologie Paris Seine (LCQB - IBPS), 75005 Paris, France.
authors: Antoine
authors: A
authors: Vallot
authors: Institut Jacques Monod, CNRS, UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, F-75205 Paris, France.
authors: Jérôme O
authors: JO
authors: Rouvière
authors: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie computationnelle et quantitative - Institut de Biologie Paris Seine (LCQB - IBPS), 75005 Paris, France.
authors: Athenaïs
authors: A
authors: Gerber
authors: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie computationnelle et quantitative - Institut de Biologie Paris Seine (LCQB - IBPS), 75005 Paris, France.
authors: Sandra
authors: S
authors: Brochet
authors: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie computationnelle et quantitative - Institut de Biologie Paris Seine (LCQB - IBPS), 75005 Paris, France.
authors: Marion
authors: M
authors: Plessis
authors: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie computationnelle et quantitative - Institut de Biologie Paris Seine (LCQB - IBPS), 75005 Paris, France.
authors: Roxane
authors: R
authors: Roquigny
authors: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie computationnelle et quantitative - Institut de Biologie Paris Seine (LCQB - IBPS), 75005 Paris, France.
authors: Jawad
authors: J
authors: Merhej
authors: Proteomics facility, Institut Jacques Monod, CNRS, UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, F-75205 Paris, France.
authors: Thibaut
authors: T
authors: Leger
authors: Proteomics facility, Institut Jacques Monod, CNRS, UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, F-75205 Paris, France.
authors: Camille
authors: C
authors: Garcia
authors: Institut Jacques Monod, CNRS, UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, F-75205 Paris, France.
authors: Gaëlle
authors: G
authors: Lelandais
authors: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie computationnelle et quantitative - Institut de Biologie Paris Seine (LCQB - IBPS), 75005 Paris, France.
authors: Elodie
authors: E
authors: Laine
authors: Institut Jacques Monod, CNRS, UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, F-75205 Paris, France.
authors: Benoit
authors: B
authors: Palancade
authors: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie computationnelle et quantitative - Institut de Biologie Paris Seine (LCQB - IBPS), 75005 Paris, France.
authors: Frédéric
authors: F
authors: Devaux
authors: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie computationnelle et quantitative - Institut de Biologie Paris Seine (LCQB - IBPS), 75005 Paris, France mathilde.garcia@upmc.fr.
authors: Mathilde
authors: M
authors: Garcia
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000626:Aminopeptidases / Q000737:chemistry / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D010441:Peptide Chain Elongation, Translational; D011485:Protein Binding; D014176:Protein Biosynthesis; D006180:Proton-Translocating ATPases / Q000235:genetics; D034443:RNA Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D000626:Aminopeptidases; D006180:Proton-Translocating ATPases; C471230:ATP2 protein, S cerevisiae; D015032:Zinc