title: Maximal Oxygen Consumption Is Reduced in Aquaporin-1 Knockout Mice.
abstract: We have measured maximal oxygen consumption ([Formula: see text]O2,max) of mice lacking one or two of the established mouse red-cell CO2 channels AQP1, AQP9, and Rhag. We intended to study whether these proteins, by acting as channels for O2, determine O2 exchange in the lung and in the periphery. We found that [Formula: see text]O2,max as determined by the Helox technique is reduced by ~16%, when AQP1 is knocked out, but not when AQP9 or Rhag are lacking. This figure holds for animals respiring normoxic as well as hypoxic gas mixtures. To see whether the reduction of [Formula: see text]O2,max is due to impaired O2 uptake in the lung, we measured carotid arterial O2 saturation (SO2) by pulse oximetry. Neither under normoxic (inspiratory O2 21%) nor under hypoxic conditions (11% O2) is there a difference in SO2 between AQP1null and WT mice, suggesting that AQP1 is not critical for O2 uptake in the lung. The fact that the % reduction of [Formula: see text]O2,max is identical in normoxia and hypoxia indicates moreover that the limitation of [Formula: see text]O2,max is not due to an O2 diffusion problem, neither in the lung nor in the periphery. Instead, it appears likely that AQP1null animals exhibit a reduced [Formula: see text]O2,max due to the reduced wall thickness and muscle mass of the left ventricles of their hearts, as reported previously. We conclude that very likely the properties of the hearts of AQP1 knockout mice cause a reduced maximal cardiac output and thus cause a reduced [Formula: see text]O2,max, which constitutes a new phenotype of these mice.
authors: Vegetative Physiologie 4220, Abt. Molekular-und Zellphysiologie, Medizinische Hochschule Hannover Hannover, Germany.
authors: Samer
authors: S
authors: Al-Samir
authors: Institut National de la Transfusion Sanguine-Institut National de la Santé et de la Recherche Médicale UMR_S1134 Paris, France.
authors: Dominique
authors: D
authors: Goossens
authors: Institut National de la Transfusion Sanguine-Institut National de la Santé et de la Recherche Médicale UMR_S1134 Paris, France.
authors: Jean-Pierre
authors: JP
authors: Cartron
authors: Biomedicine, Department Health Science and Technology, Aalborg University Aalborg, Denmark.
authors: Søren
authors: S
authors: Nielsen
authors: Institut für Zoologie, Tierärztliche Hochschule Hannover Hannover, Germany.
authors: Frank
authors: F
authors: Scherbarth
authors: Institut für Zoologie, Tierärztliche Hochschule Hannover Hannover, Germany.
authors: Stephan
authors: S
authors: Steinlechner
authors: Vegetative Physiologie 4220, Abt. Molekular-und Zellphysiologie, Medizinische Hochschule Hannover Hannover, Germany.
authors: Gerolf
authors: G
authors: Gros
authors: Vegetative Physiologie 4220, Abt. Molekular-und Zellphysiologie, Medizinische Hochschule Hannover Hannover, Germany.
authors: Volker
authors: V
authors: Endeward
keywords: Rhesus-associated glycoprotein; aquaporin 1; aquaporin 9; arterial oxygen saturation; cardiac function of the heart of aquaporin-1-deficient mice; knockout mice; maximal oxygen consumption