title: Influence of phytochemicals in piper betle linn leaf extract on wound healing.
abstract: BACKGROUND
  Wound healing has being extensively investigated over the world. Healing impairment is caused by many reasons including increasing of free-radicals-mediated damage, delaying in granulation tissue formation, reducing in angiogenesis and decreasing in collagen reorganization. These facts consequently lead to chronic wound healing. Piper betle Linn (Betle) leaves have been folklore used as an ingredient of drugs for cutaneous wound treatment. However, the effect of betle leaf on wound healing is not yet well elucidated. In this study, we aimed to investigate the healing efficacy of methanol leaf extract of Piper betle Linn on proliferation of fibroblast NIH3T3 cells as well as full-thickness burn and excision wounds in swiss mice.
  
  
  METHODS
  Scratch wound healing assays were conducted to examine the effects of betle leaf extract on healing activity of fibroblast cells. Burn and excision wounds on swiss mouse skins were created for investigating the wound healing progress caused by the betle leaf extract. Malondialdehyde (MDA) was also evaluated to examine the products of lipid hydroperoxide (LPO) under conditions of with or without betle leaf extract treatment.
  
  
  RESULTS
  The results of this study showed that Piper betle Linn leaf extract in methanol increased proliferation of NIH3T3 cells and promoted wound healing in vitro and in vivo with both burn wound and excision wound models. In addition, this extract significant decreased level of malondialdehyde (MDA) in liver of treated-mice compared with that in non-treated mice.
  
  
  CONCLUSIONS
  Our results suggest that Piper betle Linn can be used as an ingredient in developing natural origin drugs for treatment of cutaneous wounds.
authors: Department of Biochemistry and Plant Physiology, VNU University of Science, Vietnam National University, 334 Nguyen Trai St., Thanh Xuan District, Hanoi, Vietnam.
authors: Le Thi
authors: LT
authors: Lien
authors: Department of Biochemistry and Plant Physiology, VNU University of Science, Vietnam National University, 334 Nguyen Trai St., Thanh Xuan District, Hanoi, Vietnam.
authors: Nguyen Thi
authors: NT
authors: Tho
authors: Department of Biochemistry and Plant Physiology, VNU University of Science, Vietnam National University, 334 Nguyen Trai St., Thanh Xuan District, Hanoi, Vietnam.|Key laboratory of Enzyme and Protein Technology (KLEPT), VNU University of Science, Vietnam National University, Hanoi, Vietnam.
authors: Do Minh
authors: DM
authors: Ha
authors: Department of Biochemistry and Plant Physiology, VNU University of Science, Vietnam National University, 334 Nguyen Trai St., Thanh Xuan District, Hanoi, Vietnam.|Key laboratory of Enzyme and Protein Technology (KLEPT), VNU University of Science, Vietnam National University, Hanoi, Vietnam.
authors: Pham Luong
authors: PL
authors: Hang
authors: Department of Biochemistry and Plant Physiology, VNU University of Science, Vietnam National University, 334 Nguyen Trai St., Thanh Xuan District, Hanoi, Vietnam.|Key laboratory of Enzyme and Protein Technology (KLEPT), VNU University of Science, Vietnam National University, Hanoi, Vietnam.
authors: Phan Tuan
authors: PT
authors: Nghia
authors: Department of Biochemistry and Plant Physiology, VNU University of Science, Vietnam National University, 334 Nguyen Trai St., Thanh Xuan District, Hanoi, Vietnam.|Key laboratory of Enzyme and Protein Technology (KLEPT), VNU University of Science, Vietnam National University, Hanoi, Vietnam.
authors: Nguyen Dinh
authors: ND
authors: Thang
keywords: Fibroblast (NIH3T3); Leaf extract; Malondialdehyde (MDA); Piper betle Linn; Wound healing