title: Sero-Molecular Epidemiology of Japanese Encephalitis in Zhejiang, an Eastern Province of China.
abstract: Sporadic Japanese encephalitis (JE) cases still have been reported in Zhejiang Province in recent years, and concerns about vaccine cross-protection and population-level immunity have been raised off and on within the public health sphere. Genotype I (GI) has replaced GIII as the dominant genotype in Asian countries during the past few decades, which caused considerable concerns about the potential change of epidemiology characteristics and the vaccine effectiveness. The aim of this study was to investigate the prevalence of JE neutralizing antibody and its waning antibody trend after live attenuated JE vaccine immunization. Additionally, this study analyzed the molecular characteristics of the E gene of Zhejiang Japanese encephalitis virus (JEV) strains, and established genetic relationships with other JEV strains.
  
  
  
  A total of 570 serum specimens were sampled from community population aged from 0 to 92 years old in Xianju county of Zhejiang Province in 2013-2014. Microseroneutralization test results were analyzed to estimate the population immunity and to observe antibody dynamics in vaccinated children. E genes of 28 JEV strains isolated in Zhejiang Province were sequenced for phylogenetic tree construction and molecular characteristics analysis with other selected strains. Positive JE neutralizing antibody rates were higher in residents ≥35 years old (81%~98%) and lower in residents <35 years old (0~57%). 7 or 8 years after the 2nd live attenuated vaccine dose, the antibodies against for 4 different strains with microseroneutralization test were decreased by 55%~73% on seropositive rates and by 25%~38% on GMTs respectively. JEV strains isolated in recent years were all grouped into GI, while those isolated in the 1980s belonged to GIII. On important amino acid sites related to antigenicity, there was no divergence between the Zhejiang JE virus strains and the vaccine strain (SA14-14-2).
  
  
  
  JE neutralizing antibody positive rates increase in age ≥10 years old population, likely reflecting natural infection or natural boosting of immunity through exposure to wild virus. JE seropositivity rates were quite low in <35 years old age groups in Zhejiang Province. Waning of neutralizing antibody after live attenuated vaccine immunization was observed, but the clinical significance should be further investigated. Both the peripheral antibody response and genetic characterization indicate that current live attenuated JE vaccine conferred equal neutralizing potency against GI or GIII of wild strains. GI has replaced GIII as the dominant genotype in Zhejiang in the past few decades. Although the chance of exposure to wild JE virus has reduced, the virus still circulates in nature; therefore, it is necessary to implement immunization program for children continually and to conduct surveillance activity periodically.
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors: Jin-Ren
authors: 0000-0001-7062-0887
authors: JR
authors: Pan
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors: Ju-Ying
authors: JY
authors: Yan
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors: Jia-Yue
authors: JY
authors: Zhou
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors: Xue-Wen
authors: XW
authors: Tang
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors: Han-Qing
authors: HQ
authors: He
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors: Rong-Hui
authors: RH
authors: Xie
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors: Hai-Yan
authors: HY
authors: Mao
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors: Yan-Jun
authors: YJ
authors: Zhang
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors: Shu-Yun
authors: SY
authors: Xie
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D001208:Asia / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000276:immunology; D004672:Encephalitis, Japanese / Q000453:epidemiology* / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D005260:Female; D005814:Genes, Viral; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D022321:Japanese Encephalitis Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology; D055815:Young Adult
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D022321:Japanese Encephalitis Vaccines; D012367:RNA, Viral; D014613:Vaccines, Attenuated