title: Transglutaminase catalyzed hydrolyzed wheat gliadin grafted with chitosan oligosaccharide and its characterization.
abstract: Chitosan oligosaccharide (COS) was grafted on hydrolyzed wheat gliadin (HWG) with microbial transglutaminase (MTGase) as catalyst. The grafting reaction exhibited the best performance when it was done under the optimum temperature 50°C for 50min with HWG/COS mass ratio of 40:1, pH 6.00-6.50. The maximum grafting rate of COS was 64.83% at this condition. The chemical structure characterizations of HWG-COS performed by FTIR, (13)C NMR, X-ray diffractometry and TGA-DTG illustrated that amino groups in COS had participated in the formations of the amino band during the synthesis. HPLC and GFC analysis showed that HWG-COS had two main components, which together accounted for 80.64% of the total polymer and the molecular weight of the two components was 61.77kDa and 27.29kDa, respectively. HWG-COS was undissolved in water and many organic solvent, slightly soluble in 1% NaOH, with a solubility of 1.84mg/L. In antibacterial activity test, HWG-COS showed the best antimicrobial properties to Salmonella enteriditis, with an antibacterial activity improved by 41.74%.
authors: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044,China.
authors: Wen
authors: W
authors: Jiang
authors: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044,China.
authors: Zhiming
authors: Z
authors: Zhou
authors: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044,China. Electronic address: dwang@cqu.edu.cn.
authors: Dan
authors: D
authors: Wang
authors: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044,China.
authors: Xiaohua
authors: X
authors: Zhou
authors: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044,China.
authors: Renyou
authors: R
authors: Tao
authors: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044,China.
authors: Yang
authors: Y
authors: Yang
authors: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044,China.
authors: Yexin
authors: Y
authors: Shi
authors: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044,China.
authors: Guiluo
authors: G
authors: Zhang
authors: IMDEA Materials Institute, C/Eric Kandel, 2, Getafe, Madrid 28906, Spain.
authors: Deyi
authors: D
authors: Wang
authors: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044,China.
authors: Zhen
authors: Z
authors: Zhou
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000201:enzymology; D002384:Catalysis; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D005903:Gliadin / Q000737:chemistry* / Q000494:pharmacology; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry* / Q000494:pharmacology; D012475:Salmonella / Q000187:drug effects; D012480:Salmonella Infections / Q000188:drug therapy; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D011503:Transglutaminases / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; D005903:Gliadin; D048271:Chitosan; D011503:Transglutaminases
keywords: Antibacterial; Chitosan oligosaccharide; Hydrolyzed wheat gliadin; Transglutaminase