title: Prevalence of Quinolone Resistance in Enterobacteriaceae from Sierra Leone and the Detection of qnrB Pseudogenes and Modified LexA Binding Sites.
abstract: A collection of 74 Enterobacteriaceae isolates found in Bo, Sierra Leone, were tested for quinolone antibiotic susceptibility and resistance mechanisms. The majority of isolates (62%) were resistant to quinolones, and 61% harbored chromosomal gyrA and/or parC mutations. Plasmid-mediated quinolone resistance genes were ubiquitous, with qnrB and aac(6')-Ib-cr being the most prevalent. Mutated LexA binding sites were found in all qnrB1 genes, and truncated qnrB pseudogenes were found in the majority of Citrobacter isolates.
authors: Center for Bio/Medical Science and Engineering, Naval Research Laboratory, Washington, DC, USA tomasz.leski@nrl.navy.mil.
authors: Tomasz A
authors: 0000-0001-7688-9887
authors: TA
authors: Leski
authors: Center for Bio/Medical Science and Engineering, Naval Research Laboratory, Washington, DC, USA.
authors: Michael G
authors: MG
authors: Stockelman
authors: Mercy Hospital Research Laboratory, Bo, Sierra Leone.
authors: Umaru
authors: U
authors: Bangura
authors: Thomas Jefferson High School for Science and Technology, Alexandria, Virginia, USA.
authors: Daniel
authors: D
authors: Chae
authors: Mercy Hospital Research Laboratory, Bo, Sierra Leone.|Institute of Environmental Management and Quality Control, Njala University, Bo, Sierra Leone.
authors: Rashid
authors: R
authors: Ansumana
authors: Center for Bio/Medical Science and Engineering, Naval Research Laboratory, Washington, DC, USA.
authors: David A
authors: DA
authors: Stenger
authors: Center for Bio/Medical Science and Engineering, Naval Research Laboratory, Washington, DC, USA.
authors: Gary J
authors: GJ
authors: Vora
authors: Center for Bio/Medical Science and Engineering, Naval Research Laboratory, Washington, DC, USA.
authors: Chris R
authors: CR
authors: Taitt
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics*; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D011544:Pseudogenes; D015363:Quinolones / Q000494:pharmacology*; D012697:Serine Endopeptidases / Q000378:metabolism*; D012807:Sierra Leone / Q000453:epidemiology; D000099160:LexA Repressor Protein
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D027081:DNA Gyrase; D027101:DNA Topoisomerase IV; D015363:Quinolones; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein