title: Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole.
abstract: Centrioles surrounded by pericentriolar material (PCM) serve as the core structure of the centrosome. A newly formed daughter centriole grows into a functional mother centriole. However, the underlying mechanisms remain poorly understood. Here we show that Cep295, an evolutionarily conserved protein, is required for generation of a bona fide mother centriole organizing a functional centrosome. We find that Cep295 is recruited to the proximal centriole wall in the early stages of procentriole assembly. Cep295 then acts as a scaffold for the proper assembly of the daughter centriole. We also find that Cep295 binds directly to and recruits Cep192 onto the daughter centriole wall, which presumably endows the function of the new mother centriole for PCM assembly, microtubule-organizing centre activity and the ability for centriole formation. These findings led us to propose that Cep295 acts upstream of the conserved pathway for centriole formation and promotes the daughter-to-mother centriole conversion.
authors: Department of Molecular Genetics, Division of Centrosome Biology, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.|Department of Genetics, School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), Mishima, Shizuoka 411-8540, Japan.
authors: Yuki
authors: Y
authors: Tsuchiya
authors: Department of Molecular Genetics, Division of Centrosome Biology, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.
authors: Satoko
authors: S
authors: Yoshiba
authors: Department of Molecular Genetics, Division of Centrosome Biology, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.|Department of Genetics, School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), Mishima, Shizuoka 411-8540, Japan.
authors: Akshari
authors: A
authors: Gupta
authors: Department of Molecular Genetics, Division of Centrosome Biology, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.|Department of Genetics, School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), Mishima, Shizuoka 411-8540, Japan.
authors: Koki
authors: K
authors: Watanabe
authors: Department of Molecular Genetics, Division of Centrosome Biology, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.|Department of Genetics, School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), Mishima, Shizuoka 411-8540, Japan.
authors: Daiju
authors: D
authors: Kitagawa
mesh_terms: D018797:Cell Cycle Proteins / Q000502:physiology*; D045744:Cell Line, Tumor; D002502:Centrioles / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology*; D055785:Gene Knockdown Techniques; D006801:Humans; D008869:Microtubule-Associated Proteins; D009154:Mutation; D001678:Organelle Biogenesis; D011485:Protein Binding / Q000502:physiology; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: C556019:CEP295 protein, human; D018797:Cell Cycle Proteins; C526596:Cep192 protein, human; D002868:Chromosomal Proteins, Non-Histone; D008869:Microtubule-Associated Proteins; D034741:RNA, Small Interfering