title: Regulation of Adipogenesis Through Differential Modulation of ROS and Kinase Signaling Pathways by 3,4'-Dihydroxyflavone Treatment.
abstract: Studies on adipogenesis may be important for regulating human and/or animal obesity, which causes several complications such as, type II diabetes, hypertension, and cardiovascular disease, thus giving rise to increased economic burden in many countries. Previous reports revealed that various flavonoids have anti-apoptotic, antioxidant, and cell differentiation-regulating activities with a number of physiological benefits, including protection from cardiovascular disease, cancers, and oxidative stress. As we found that the hydroxylation patterns of the flavonoid B ring are known to play a critical role in their function, we screened several flavonoids containing different numbers and positions of OH substitutions in B ring for their modulatory property on adipogenesis. In this study, we revealed the anti-adipogenic activity of the naturally derived flavonoid, 3,4'-dihydroxyflavone (3,4'-DHF) in murine 3T3-L1 pre-adipocytes and equine adipose-derived stromal cells (eADSCs). We found that treatment with 3,4'-dihydroxyflavone (3,4'-DHF) led to decreased expression of adipogenic markers and lipid deposition with differential modulation of ROS and kinase signaling pathways. Regulation of ROS generation through the differential modulation of ROS-regulating gene expression was revealed to have an important role in the suppression of adipogenesis and increase of osteogenesis in eADSCs following 3,4'-DHF treatment. These results suggest that the flavonoid 3,4'-DHF can be used to regulate adipogenesis in ADSCs, which has potential therapeutic application in regenerative medicine or health care for humans and many sport or companion animals. J. Cell. Biochem. 118: 1065-1077, 2017. © 2016 Wiley Periodicals, Inc.
authors: Department of Stem Cell and Regenerative Biotechnology, Animal Resources Research Center, Bio-Organ Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.
authors: Jihae
authors: J
authors: Han
authors: Department of Stem Cell and Regenerative Biotechnology, Animal Resources Research Center, Bio-Organ Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.
authors: Hye Yeon
authors: HY
authors: Choi
authors: Department of Stem Cell and Regenerative Biotechnology, Animal Resources Research Center, Bio-Organ Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.
authors: Ahmed Abdal
authors: AA
authors: Dayem
authors: Department of Stem Cell and Regenerative Biotechnology, Animal Resources Research Center, Bio-Organ Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.
authors: Kyeongseok
authors: K
authors: Kim
authors: Department of Stem Cell and Regenerative Biotechnology, Animal Resources Research Center, Bio-Organ Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.
authors: Gwangmo
authors: G
authors: Yang
authors: Department of Stem Cell and Regenerative Biotechnology, Animal Resources Research Center, Bio-Organ Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.
authors: Jihye
authors: J
authors: Won
authors: Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.
authors: Sun Hee
authors: SH
authors: Do
authors: Department of Stem Cell and Regenerative Biotechnology, Animal Resources Research Center, Bio-Organ Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.
authors: Jin-Hoi
authors: JH
authors: Kim
authors: College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea.
authors: Kyu-Shik
authors: KS
authors: Jeong
authors: Department of Stem Cell and Regenerative Biotechnology, Animal Resources Research Center, Bio-Organ Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.
authors: Ssang-Goo
authors: SG
authors: Cho
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D047309:Flavones / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005819:Genetic Markers / Q000187:drug effects; D006736:Horses; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: C559991:3,4'-dihydroxyflavone; D047309:Flavones; D005819:Genetic Markers; D017382:Reactive Oxygen Species
keywords: 3,4′-DIHYDROXYFLAVONE; ADIPOGENESIS; EQUINE ADIPOSE-DERIVED STROMAL CELLS (eADSCs); HUMAN REGENERATIVE MEDICINE OR ANIMAL HEALTH CARE; ROS-REGULATING GENES EXPRESSION