title: Effect of Predatory Bacteria on Human Cell Lines.
abstract: Predatory bacteria are Gram-negative bacteria that prey on other Gram-negative bacteria and have been considered as potential therapeutic agents against multi-drug resistant pathogens. In vivo animal models have demonstrated that predatory bacteria are non-toxic and non-immunogenic in rodents. In order to consider the use of predatory bacteria as live antibiotics, it is important to investigate their effect on human cells. The aim of this study was to determine the effect of Bdellovibrio bacteriovorus strains 109J and HD100, and Micavibrio aeruginosavorus strain ARL-13 on cell viability and inflammatory responses of five human cell lines, representative of clinically relevant tissues. We found that the predators were not cytotoxic to any of the human cell lines tested. Microscopic imaging showed no signs of cell detachment, as compared to predator-free cells. In comparison to an E. coli control, exposure to higher concentrations of the predators did not trigger a significant elevation of pro-inflammatory cytokines in four of the five human cell lines tested. Our work underlines the non-pathogenic attributes of predatory bacteria on human cells and highlights their potential use as live antibiotics against human pathogens.
authors: Department of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ, United States of America.
authors: Shilpi
authors: S
authors: Gupta
authors: Department of Medicine and the Center for Emerging Pathogens, Rutgers, New Jersey Medical School, Newark, NJ, United States of America.
authors: Chi
authors: C
authors: Tang
authors: Department of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ, United States of America.
authors: Michael
authors: M
authors: Tran
authors: Department of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ, United States of America.
authors: Daniel E
authors: DE
authors: Kadouri
mesh_terms: D000070060:Bdellovibrio bacteriovorus / Q000378:metabolism*; D002460:Cell Line; D004926:Escherichia coli / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000382:microbiology