title: Hyperlipidemia induced by high-fat diet enhances dentin formation and delays dentin mineralization in mouse incisor.
abstract: Dyslipidemia has become a serious health problem in children and adolescents worldwide for its high prevalence. Since hard tissues of permanent teeth form mainly during this period and lipids are actively involved in tooth development, the effects of hyperlipidemia on dental tissue formation and mineralization need to be illustrated. In this study, hyperlipidemia model was established in mice fed with high-fat diet (HFD). Micro-CT and histomorphological analyses were performed on the mandibular bones to assess the morphological changes of the mandibular incisor and first molar. After 4 weeks of HFD feeding, mice had significantly elevated serum lipid levels compared with mice fed with control diet. After 8 weeks, the mandibular incisor presented significantly increased dentin thickness and decreased diameter of pulp cavity in HFD-fed mice compared with control diet-fed mice, while its gross morphology and enamel thickness were not altered. In the mandibular first molar, dentin thickness of root did not show difference between the two groups. Histological section showed that mandibular incisor of HFD-fed mice manifested a wider predentin region and a lower mineral apposition rate compared with that of the control mice. In conclusion, hyperlipidemia induced by HFD feeding enhances dentin formation and delays dentin mineralization in the developing mouse incisor.
authors: School of Stomatology, Shandong University, 44-1 West Wen Hua Road, Jinan, 250012, Shandong, People's Republic of China.|Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University, Jinan, 250012, Shandong, People's Republic of China.
authors: Xin
authors: X
authors: Ye
authors: School of Stomatology, Shandong University, 44-1 West Wen Hua Road, Jinan, 250012, Shandong, People's Republic of China. teadenzj@sdu.edu.cn.|Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University, Jinan, 250012, Shandong, People's Republic of China. teadenzj@sdu.edu.cn.
authors: Jin
authors: J
authors: Zhang
authors: School of Stomatology, Shandong University, 44-1 West Wen Hua Road, Jinan, 250012, Shandong, People's Republic of China. yangps@sdu.edu.cn.|Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University, Jinan, 250012, Shandong, People's Republic of China. yangps@sdu.edu.cn.
authors: Pishan
authors: http://orcid.org/0000-0002-7326-9452
authors: P
authors: Yang
mesh_terms: D000818:Animals; D015415:Biomarkers; D001835:Body Weight; D003804:Dentin / Q000378:metabolism*; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D006949:Hyperlipidemias / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D007180:Incisor / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology*; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D014074:Tooth Calcification; D014092:Tooth Root / Q000000981:diagnostic imaging / Q000378:metabolism; D055114:X-Ray Microtomography
chemical_list: D015415:Biomarkers; D008055:Lipids
keywords: Dentin mineralization; Dentinogenesis; Dyslipidemia; Hyperlipidemia; Tooth development