title: Nucleoporin-Regulated MAP Kinase Signaling in Immunity to a Necrotrophic Fungal Pathogen.
abstract: Pathogen-responsive mitogen-activated protein kinase (MAPK or MPK) cascades relay signals from activated immune receptors across the nuclear envelope to intranuclear targets. However, in plants, little is known about the spatial control of MAPK signaling. Here, we report that the Arabidopsis (Arabidopsis thaliana) nuclear pore complex protein Nup88/MOS7 is essential for immunity to the necrotrophic fungus Botrytis cinerea The mos7-1 mutation, causing a four-amino acid deletion, compromises B. cinerea-induced activation of the key immunoregulatory MAPKs MPK3/MPK6 and reduces MPK3 protein levels posttranscriptionally. Furthermore, MOS7 contributes to retaining a sufficient MPK3 abundance in the nucleus, which is required for full immunity to B. cinerea Finally, we present a structural model of MOS7 and show that the mos7-1 mutation compromises interactions with Nup98a/b, two phenylalanine-glycine repeat nucleoporins implicated in maintaining the selective nuclear pore complex permeability barrier. Together, our analysis uncovered MOS7 and Nup98 as novel components of plant immunity toward a necrotrophic pathogen and provides mechanistic insights into how these nucleoporins coordinate nucleocytoplasmic transport to mount a robust immune response.
authors: Department of Plant Cell Biology, Albrecht-von-Haller Institute for Plant Sciences, Georg-August-University Göttingen, 37077 Göttingen, Germany (B.G., C.R., M.K., M.W.);Department of Plant Biochemistry, Dahlem Centre of Plant Sciences, Institute of Biology, Freie Universität Berlin, 14195 Berlin, Germany (L.W.); andDepartment of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel (L.M., A.S.).
authors: Bianca
authors: B
authors: Genenncher
authors: Department of Plant Cell Biology, Albrecht-von-Haller Institute for Plant Sciences, Georg-August-University Göttingen, 37077 Göttingen, Germany (B.G., C.R., M.K., M.W.);Department of Plant Biochemistry, Dahlem Centre of Plant Sciences, Institute of Biology, Freie Universität Berlin, 14195 Berlin, Germany (L.W.); andDepartment of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel (L.M., A.S.).
authors: Lennart
authors: 0000-0003-0667-5343
authors: L
authors: Wirthmueller
authors: Department of Plant Cell Biology, Albrecht-von-Haller Institute for Plant Sciences, Georg-August-University Göttingen, 37077 Göttingen, Germany (B.G., C.R., M.K., M.W.);Department of Plant Biochemistry, Dahlem Centre of Plant Sciences, Institute of Biology, Freie Universität Berlin, 14195 Berlin, Germany (L.W.); andDepartment of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel (L.M., A.S.).
authors: Charlotte
authors: C
authors: Roth
authors: Department of Plant Cell Biology, Albrecht-von-Haller Institute for Plant Sciences, Georg-August-University Göttingen, 37077 Göttingen, Germany (B.G., C.R., M.K., M.W.);Department of Plant Biochemistry, Dahlem Centre of Plant Sciences, Institute of Biology, Freie Universität Berlin, 14195 Berlin, Germany (L.W.); andDepartment of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel (L.M., A.S.).
authors: Melanie
authors: M
authors: Klenke
authors: Department of Plant Cell Biology, Albrecht-von-Haller Institute for Plant Sciences, Georg-August-University Göttingen, 37077 Göttingen, Germany (B.G., C.R., M.K., M.W.);Department of Plant Biochemistry, Dahlem Centre of Plant Sciences, Institute of Biology, Freie Universität Berlin, 14195 Berlin, Germany (L.W.); andDepartment of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel (L.M., A.S.).
authors: Liang
authors: L
authors: Ma
authors: Department of Plant Cell Biology, Albrecht-von-Haller Institute for Plant Sciences, Georg-August-University Göttingen, 37077 Göttingen, Germany (B.G., C.R., M.K., M.W.);Department of Plant Biochemistry, Dahlem Centre of Plant Sciences, Institute of Biology, Freie Universität Berlin, 14195 Berlin, Germany (L.W.); andDepartment of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel (L.M., A.S.).
authors: Amir
authors: A
authors: Sharon
authors: Department of Plant Cell Biology, Albrecht-von-Haller Institute for Plant Sciences, Georg-August-University Göttingen, 37077 Göttingen, Germany (B.G., C.R., M.K., M.W.);Department of Plant Biochemistry, Dahlem Centre of Plant Sciences, Institute of Biology, Freie Universität Berlin, 14195 Berlin, Germany (L.W.); andDepartment of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv 69978, Israel (L.M., A.S.) wiermer@uni-goettingen.de.
authors: Marcel
authors: 0000-0002-1232-2707
authors: M
authors: Wiermer
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D020171:Botrytis / Q000276:immunology / Q000502:physiology; D060467:Disease Resistance / Q000235:genetics / Q000276:immunology; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions / Q000276:immunology; D015151:Immunoblotting; D020935:MAP Kinase Signaling System / Q000235:genetics*; D018613:Microscopy, Confocal; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins / Q000235:genetics* / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D057865:Plant Immunity / Q000235:genetics; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D029681:Arabidopsis Proteins; D028861:Nuclear Pore Complex Proteins; C458516:AtMPK3 protein, Arabidopsis; C436293:MPK6 protein, Arabidopsis; D020928:Mitogen-Activated Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases