title: Deep vein thrombosis is accurately predicted by comprehensive analysis of the levels of microRNA-96 and plasma D-dimer.
abstract: The aim of the present study was to investigate the association between platelet microRNA-96 (miR-96) expression levels and the occurrence of deep vein thrombosis (DVT) in orthopedic patients. A total of consecutive 69 orthopedic patients with DVT and 30 healthy individuals were enrolled. Ultrasonic color Doppler imaging was performed on lower limb veins after orthopedic surgery to determine the occurrence of DVT. An enzyme-linked fluorescent assay was performed to detect the levels of D-dimer in plasma. A quantitative polymerase chain reaction assay was performed to determine the expression levels of miR-96. Expression levels of platelet miR-96 were significantly increased in orthopedic patients after orthopedic surgery. miR-96 expression levels in orthopedic patients with DVT at days 1, 3 and 7 after orthopedic surgery were significantly increased when compared with those in the control group. The increased miR-96 expression levels were correlated with plasma D-dimer levels in orthopedic patients with DVT. However, for the orthopedic patients in the non-DVT group following surgery, miR-96 expression levels were correlated with plasma D-dimer levels. In summary, the present results suggest that the expression levels of miR-96 may be associated with the occurrence of DVT. The occurrence of DVT may be accurately predicted by comprehensive analysis of the levels of miR-96 and plasma D-dimer.
authors: Department of Orthopedics, Laiwu People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors: Xuesheng
authors: X
authors: Xie
authors: Department of Orthopedics, Laiwu People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors: Changpeng
authors: C
authors: Liu
authors: Department of Orthopedics, Laiwu People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors: Wei
authors: W
authors: Lin
authors: Department of Orthopedics, Laiwu People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors: Baoming
authors: B
authors: Zhan
authors: Department of Orthopedics, Laiwu People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors: Changjun
authors: C
authors: Dong
authors: Department of Orthopedics, Laiwu People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors: Zhen
authors: Z
authors: Song
authors: Department of Orthopedics, Laiwu People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors: Shilei
authors: S
authors: Wang
authors: Department of Orthopedics, Laiwu People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors: Yingguo
authors: Y
authors: Qi
authors: Department of Orthopedics, Laiwu People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors: Jiali
authors: J
authors: Wang
authors: Department of Orthopedics, Laiwu People's Hospital, Laiwu, Shandong 271100, P.R. China.
authors: Zengquan
authors: Z
authors: Gu
keywords: deep vein thrombosis; microRNA-96; orthopedic surgery