title: Mp1p Is a Virulence Factor in Talaromyces (Penicillium) marneffei.
abstract: Talaromyces marneffei is an opportunistic dimorphic fungus prevalent in Southeast Asia. We previously demonstrated that Mp1p is an immunogenic surface and secretory mannoprotein of T. marneffei. Since Mp1p is a surface protein that can generate protective immunity, we hypothesized that Mp1p and/or its homologs are virulence factors.
  
  
  
  We examined the pathogenic roles of Mp1p and its homologs in a mouse model. All mice died 21 and 30 days after challenge with wild-type T. marneffei PM1 and MP1 complemented mutant respectively. None of the mice died 60 days after challenge with MP1 knockout mutant (P<0.0001). Seventy percent of mice died 60 days after challenge with MP1 knockdown mutant (P<0.0001). All mice died after challenge with MPLP1 to MPLP13 knockdown mutants, suggesting that only Mp1p plays a significant role in virulence. The mean fungal loads of PM1 and MP1 complemented mutant in the liver, lung, kidney and spleen were significantly higher than those of the MP1 knockout mutant. Similarly, the mean load of PM1 in the liver, lung and spleen were significantly higher than that of the MP1 knockdown mutant. Histopathological studies showed an abundance of yeast in the kidney, spleen, liver and lung with more marked hepatic and splenic necrosis in mice challenged with PM1 compared to MP1 knockout and MP1 knockdown mutants. Likewise, a higher abundance of yeast was observed in the liver and spleen of mice challenged with MP1 complemented mutant compared to MP1 knockout mutant. PM1 and MP1 complemented mutant survived significantly better than MP1 knockout mutant in macrophages at 48 hours (P<0.01) post-infection. The mean fungal counts of Pichia pastoris GS115-MP1 in the liver (P<0.001) and spleen (P<0.05) of mice were significantly higher than those of GS115 at 24 hours post-challenge.
  
  
  
  Mp1p is a key virulence factor of T. marneffei. Mp1p mediates virulence by improving the survival of T. marneffei in macrophages.
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.|State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong.|Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong.|Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong.
authors: Patrick C Y
authors: PC
authors: Woo
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.|State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong.|Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong.|Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong.
authors: Susanna K P
authors: SK
authors: Lau
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.
authors: Candy C Y
authors: CC
authors: Lau
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.
authors: Edward T K
authors: ET
authors: Tung
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.
authors: Ken T K
authors: KT
authors: Chong
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.
authors: Fengjuan
authors: F
authors: Yang
authors: School of Biomedical Sciences, The University of Hong Kong, Hong Kong.
authors: Hongmin
authors: H
authors: Zhang
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.
authors: Raymond K C
authors: RK
authors: Lo
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.
authors: Jian-Pao
authors: JP
authors: Cai
authors: Department of Pathology, The University of Hong Kong, Hong Kong.
authors: Rex K H
authors: RK
authors: Au-Yeung
authors: Department of Pathology, United Christian Hospital and Tseung Kwan O Hospital, Hong Kong.
authors: Wing-Fung
authors: WF
authors: Ng
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.|State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong.|Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong.|Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong.
authors: Herman
authors: H
authors: Tse
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.|State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong.|Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong.|Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong.
authors: Samson S Y
authors: SS
authors: Wong
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.
authors: Simin
authors: S
authors: Xu
authors: School of Biomedical Sciences, The University of Hong Kong, Hong Kong.
authors: Wai Hei
authors: WH
authors: Lam
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.
authors: Man-Kit
authors: MK
authors: Tse
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.|State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong.|Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong.|Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong.
authors: Kong Hung
authors: KH
authors: Sze
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.|State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong.|Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong.|Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong.
authors: Richard Y
authors: RY
authors: Kao
authors: Department of Medicine, University of British Columbia, Vancouver, Canada.
authors: Neil E
authors: NE
authors: Reiner
authors: School of Biomedical Sciences, The University of Hong Kong, Hong Kong.
authors: Quan
authors: Q
authors: Hao
authors: Department of Microbiology, The University of Hong Kong, Hong Kong.|State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong.|Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong.|Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong.
authors: Kwok-Yung
authors: KY
authors: Yuen
mesh_terms: D000818:Animals; D000946:Antigens, Fungal / Q000235:genetics / Q000276:immunology; D055785:Gene Knockdown Techniques; D006801:Humans; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008168:Lung / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D009154:Mutation; D009181:Mycoses / Q000276:immunology; D010843:Pichia / Q000254:growth & development / Q000502:physiology; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D032901:Talaromyces / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D037521:Virulence Factors / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*
chemical_list: D000946:Antigens, Fungal; D008562:Membrane Glycoproteins; D037521:Virulence Factors; C025363:mannoproteins