title: The Relationship of Cytokines IL-13 and IL-17 with Autoantibodies Profile in Early Rheumatoid Arthritis.
abstract: Aims. In the present study, we aimed to assess the concentrations of IL-13 and IL-17 in serum of patients with early rheumatoid arthritis (eRA), the investigation of correlation between the concentrations of these cytokines and disease activity score, and the concentration of some autoantibodies and the evaluation of the utility of IL-13 and -17 concentration measurements as markers of disease activity. Materials and Methods. Serum samples were collected from 30 patients and from 28 controls and analysed parameters. Results. The serum concentrations of IL-13, IL-17, anti-CCP, and IgM-RF were statistically significantly higher in patients with eRA, compared to the controls. IL-13 concentrations in the severe and moderate groups with eRA were statistically higher than in the mild and control groups. Also, in the case of IL-17, serum concentrations increased proportionally with the disease activity of eRA. We observe that concentrations of IL-13 and -17 did not correlate with autoantibodies. IL-17 concentration significantly positively correlated with CRP, while IL-13 concentration significantly negatively correlated with CRP. Disease activity score, DAS28, was strongly positively correlated with levels of ESR and weakly positively correlated with concentrations of anti-RA33 autoantibodies. IL-13 has a higher diagnostic utility than IL-17, CRP, ESR, IgM-RF, and anti-CCP as markers of disease activity. Conclusions. The presence of higher IL-13 and IL-17 serum levels in patients, compared with those of controls, confirms that these markers, found with high specificity, might be involved in the pathogenesis of eRA. IL-13 and IL-17 might be of better usefulness in the prediction of eRA activity status than IgM-RF and anti-CCP.
authors: Department of Immunology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
authors: Isabela
authors: I
authors: Siloşi
authors: Department of Immunology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
authors: Mihail Virgil
authors: MV
authors: Boldeanu
authors: Department of Physiology, Titu Maiorescu University of Bucharest, 187 Calea Vacaresti Street, 004051 Bucharest, Romania.
authors: Manole
authors: M
authors: Cojocaru
authors: Department of Medical Semiology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
authors: Viorel
authors: 0000-0002-9451-6969
authors: V
authors: Biciuşcă
authors: Department of Medical Semiology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
authors: Vlad
authors: 0000-0001-7398-5689
authors: V
authors: Pădureanu
authors: Department of Pharmacology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
authors: Maria
authors: 0000-0001-8089-1787
authors: M
authors: Bogdan
authors: Medico Science SRL, Stem Cell Bank Unit, 1B Brazda lui Novac Street, 200690 Craiova, Romania.
authors: Ramona Georgiana
authors: RG
authors: Badea
authors: Department of Microbiology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
authors: Carmen
authors: 0000-0002-8694-8374
authors: C
authors: Avramescu
authors: Department of Pediatrics, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
authors: Ileana Octavia
authors: 0000-0003-1653-603X
authors: IO
authors: Petrescu
authors: Department of Medical Semiology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
authors: Florin
authors: 0000-0001-9027-6489
authors: F
authors: Petrescu
authors: Department of Surgery, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania.
authors: Cristian A
authors: 0000-0001-6282-7052
authors: CA
authors: Siloşi
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000175:diagnosis / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D015415:Biomarkers; D016207:Cytokines / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D018793:Interleukin-13 / Q000097:blood*; D020381:Interleukin-17 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D012720:Severity of Illness Index
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D016207:Cytokines; D007074:Immunoglobulin G; D018793:Interleukin-13; D020381:Interleukin-17