title: New water soluble Hg2+selective fluorescent calix[4]arenes: Synthesis and application in living cells imaging.
abstract: The present study demonstrates the synthesis of water-soluble fluorescent calix[4]arenes (6 and 7) and its application in living cell imaging for Hg2+detection at a low level. The synthesized fluorescent ligands 6 and 7 were characterized by1H NMR technique. The fluorescent study showed both water soluble ligands were Hg2+selective and follow photo-induced electron transfer (PET) process. From the fluorimeter titration experiment detection limit was calculated as 1.14×10-5and 3.42×10-5for ligand 6 and 7, respectively. From the Benesi-Hildebrand plot binding constant values were evaluated as 666.7 and 733.3M-1for 6 and 7, respectively. The interactions between ligands 6 and 7 and Hg2+were also demonstrated in living cells, SW-620, using Fluorescent Cell Imager. While ligands 6 and 7 alone show fluorescent properties, they loss their action with the presence of Hg2+in SW-620 cells.
authors: Selcuk University, Department of Chemistry, 42031 Konya, Turkey.
authors: Mehmet
authors: M
authors: Oguz
authors: Selcuk University, Department of Chemistry, 42031 Konya, Turkey; National Center of Excellence in Analytical Chemistry University of Sindh, Jamshoro 76080, Pakistan.
authors: Asif Ali
authors: AA
authors: Bhatti
authors: Selcuk University, Department of Biochemistry, Konya 42031, Turkey.
authors: Serdar
authors: S
authors: Karakurt
authors: Selcuk University, Department of Chemistry, 42031 Konya, Turkey.
authors: Mehmet
authors: M
authors: Aktas
authors: Selcuk University, Department of Chemistry, 42031 Konya, Turkey. Electronic address: myilmaz42@yahoo.com.
authors: Mustafa
authors: M
authors: Yilmaz
mesh_terms: D047250:Calixarenes / Q000138:chemical synthesis / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival; D005453:Fluorescence; D006801:Humans; D021621:Imaging, Three-Dimensional; D007700:Kinetics; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water; D047250:Calixarenes
keywords: Dansyl; Fluorescence; Living cell imaging; p-Sulphonatocalix[4]arene