title: Odorant-Binding Proteins and Chemosensory Proteins from an Invasive Pest Lissorhoptrus oryzophilus (Coleoptera: Curculionidae).
abstract: The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), is a serious pest species both in its original distribution region of northern America and its invaded regions of eastern Asia and southern Europe. The odorant-binding proteins (OBPs) and the chemosensory proteins (CSPs) play important roles in host and mate locating, thus might play a significant role in the success of the species as an invader, which has not been characterized yet. We identified 10 OBPs and 5 CSPs in L. oryzophilus and investigated the expression profiles of these genes in various tissues by quantitative real-time PCR. Five classic OBPs were predominantly expressed in the antennae. CSPs were expressed ubiquitously with particularly high transcript levels in antennae, legs, and wings. Three antenna-specific OBPs (LoOBP1, 8, 11) were up-regulated following 1-3 d of food deprivation and down-regulated afterward. These findings suggest most classic OBPs are likely involved in chemoreception whereas CSPs as well as the minus-C OBPs may have broader physiological functions, which in turn may help to understand the molecular aspects of chemical communication in this invasive insect.
authors: State Key Laboratory of Rice Biology and Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, China (yuanxin@zju.edu.cn; jyd@zju.edu.cn; wangguiyao@yeah.net; hangyubig@qq.com; joewenwu@163.com; suliu@ahau.edu.cn; jacheng@zju.edu.cn; ggurr@csu.edu.au; zrzhu@zju.edu.cn).
authors: Xin
authors: X
authors: Yuan
authors: State Key Laboratory of Rice Biology and Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, China (yuanxin@zju.edu.cn; jyd@zju.edu.cn; wangguiyao@yeah.net; hangyubig@qq.com; joewenwu@163.com; suliu@ahau.edu.cn; jacheng@zju.edu.cn; ggurr@csu.edu.au; zrzhu@zju.edu.cn).
authors: Yan-Dong
authors: YD
authors: Jiang
authors: State Key Laboratory of Rice Biology and Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, China (yuanxin@zju.edu.cn; jyd@zju.edu.cn; wangguiyao@yeah.net; hangyubig@qq.com; joewenwu@163.com; suliu@ahau.edu.cn; jacheng@zju.edu.cn; ggurr@csu.edu.au; zrzhu@zju.edu.cn).
authors: Gui-Yao
authors: GY
authors: Wang
authors: State Key Laboratory of Rice Biology and Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, China (yuanxin@zju.edu.cn; jyd@zju.edu.cn; wangguiyao@yeah.net; hangyubig@qq.com; joewenwu@163.com; suliu@ahau.edu.cn; jacheng@zju.edu.cn; ggurr@csu.edu.au; zrzhu@zju.edu.cn).
authors: Hang
authors: H
authors: Yu
authors: State Key Laboratory of Rice Biology and Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, China (yuanxin@zju.edu.cn; jyd@zju.edu.cn; wangguiyao@yeah.net; hangyubig@qq.com; joewenwu@163.com; suliu@ahau.edu.cn; jacheng@zju.edu.cn; ggurr@csu.edu.au; zrzhu@zju.edu.cn).
authors: Wen-Wu
authors: WW
authors: Zhou
authors: State Key Laboratory of Rice Biology and Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, China (yuanxin@zju.edu.cn; jyd@zju.edu.cn; wangguiyao@yeah.net; hangyubig@qq.com; joewenwu@163.com; suliu@ahau.edu.cn; jacheng@zju.edu.cn; ggurr@csu.edu.au; zrzhu@zju.edu.cn).
authors: Su
authors: S
authors: Liu
authors: Institute of Entomology, Guizhou University, Guiyang, China (yangmaofa@sohu.com).
authors: Mao-Fa
authors: MF
authors: Yang
authors: State Key Laboratory of Rice Biology and Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, China (yuanxin@zju.edu.cn; jyd@zju.edu.cn; wangguiyao@yeah.net; hangyubig@qq.com; joewenwu@163.com; suliu@ahau.edu.cn; jacheng@zju.edu.cn; ggurr@csu.edu.au; zrzhu@zju.edu.cn).
authors: Jiaan
authors: J
authors: Cheng
authors: State Key Laboratory of Rice Biology and Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, China (yuanxin@zju.edu.cn; jyd@zju.edu.cn; wangguiyao@yeah.net; hangyubig@qq.com; joewenwu@163.com; suliu@ahau.edu.cn; jacheng@zju.edu.cn; ggurr@csu.edu.au; zrzhu@zju.edu.cn) Graham Centre for Agricultural Innovation, Charles Sturt University, Orange, NSW, Australia.
authors: Geoff M
authors: GM
authors: Gurr
authors: Texas A&M AgriLife Research and Extension Center, Beaumont, TX (moway@aesrg.TAMU.edu).
authors: Michael O
authors: MO
authors: Way
authors: State Key Laboratory of Rice Biology and Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, China (yuanxin@zju.edu.cn; jyd@zju.edu.cn; wangguiyao@yeah.net; hangyubig@qq.com; joewenwu@163.com; suliu@ahau.edu.cn; jacheng@zju.edu.cn; ggurr@csu.edu.au; zrzhu@zju.edu.cn) zrzhu@zju.edu.cn.
authors: Zeng-Rong
authors: ZR
authors: Zhu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005260:Female; D005508:Food Deprivation; D015870:Gene Expression; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D058865:Introduced Species; D010802:Phylogeny; D012313:RNA / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D018035:Receptors, Odorant / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D040681:Structural Homology, Protein; D048889:Weevils / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D018035:Receptors, Odorant; C045648:odorant-binding protein; D012313:RNA
keywords: chemical communication; host location; rice water weevil