title: Design and development of pH-responsive HSPC:C12H25-PAA chimeric liposomes.
abstract: The application of stimuli-responsive medical practices has emerged, in which pH-sensitive liposomes figure prominently. This study investigates the impact of the incorporation of different amounts of pH-sensitive polymer, C12H25-PAA (poly(acrylic acid) with a hydrophobic end group) in l-α-phosphatidylcholine, hydrogenated (Soy) (HSPC) phospholipidic bilayers, with respect to biomimicry and functionality. PAA is a poly(carboxylic acid) molecule, classified as a pH-sensitive polymer, whose pH-sensitivity is attributed to its regulative -COOH groups, which are protonated under acidic pH (pKa ∼4.2). Our concern was to fully characterize, in a biophysical and thermodynamical manner, the mixed nanoassemblies arising from the combination of the two biomaterials. At first, we quantified the physicochemical characteristics and physical stability of the prepared chimeric nanosystems. Then, we studied their thermotropic behavior, through measurement of thermodynamical parameters, using Differential Scanning Calorimetry (DSC). Finally, the loading and release of indomethacin (IND) were evaluated, as well as the physicochemical properties and stability of the nanocarriers incorporating it. As expected, thermodynamical findings are in line with physicochemical results and also explain the loading and release profiles of IND. The novelty of this investigation is the utilization of these pH-sensitive chimeric advanced Drug Delivery nano Systems (aDDnSs) in targeted drug delivery which relies entirely on the biophysics and thermodynamics between such designs and the physiological membranes and environment of living organisms.
authors: a Department of Pharmaceutical Technology, Faculty of Pharmacy , National and Kapodistrian University of Athens , Athens , Greece and.
authors: Nikolaos
authors: N
authors: Naziris
authors: a Department of Pharmaceutical Technology, Faculty of Pharmacy , National and Kapodistrian University of Athens , Athens , Greece and.|b Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation , Athens , Greece.
authors: Natassa
authors: N
authors: Pippa
authors: b Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation , Athens , Greece.
authors: Anastasia
authors: A
authors: Meristoudi
authors: b Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation , Athens , Greece.
authors: Stergios
authors: S
authors: Pispas
authors: a Department of Pharmaceutical Technology, Faculty of Pharmacy , National and Kapodistrian University of Athens , Athens , Greece and.
authors: Costas
authors: C
authors: Demetzos
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D015195:Drug Design; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000138:chemical synthesis / Q000737:chemistry; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; C006903:carbopol 940
keywords: Differential Scanning Calorimetry; PAA; drug release; indomethacin; pH-responsive liposomes