title: The CD47 "don't eat me signal" is highly expressed in human ovarian cancer.
abstract: OBJECTIVE
  The CD47 "don't eat me" signal allows tumor immune evasion. We tested the association of CD47 expression with outcomes in EOC.
  
  
  METHODS
  CD47 expression was examined within the TCGA database for ovarian carcinoma. For validation, IHC was performed on a TMA consisting of specimens from 265 patients with EOC. The medical records of the patients were also retrospectively reviewed to correlate demographic and survival data.
  
  
  RESULTS
  CD47 was amplified in 15/316 (5%) ovarian serous cancers in TCGA. In the validation cohort, the majority of patients had stage III/IV disease (208/265, 78.4%). CD47 expression was seen in 210/265 (79.2%). Patients were categorized into CD47hi (129/265; 48.7%) versus CD47lo (136/265; 51.3%). Patients with CD47lo tumors were more likely to have a complete response to adjuvant therapy than CD47hi (65% vs 50%, p=0.026). Although there was a trend towards an increase in median OS (37.64 vs 45.26months, p=0.92) in the CD47lo group compared with CD47hi, the difference was not significant.
  
  
  CONCLUSIONS
  CD47 is expressed at high frequency in EOC. Patients with CD47lo EOC had a better treatment response to standard therapy, and trended towards improved OS. This demonstrates that while CD47 may be an immunologic shield that may be considered for targeted therapies, it is likely that it operates in concert with other mechanisms of immune evasion. Future studies to evaluate CD47 expression with other known mechanisms of immune escape in the tumor microenvironment may help further define its role.
authors: Roswell Park Cancer Institute, Department of Gynecologic Oncology, USA; Roswell Park Cancer Institute, Center for Immunotherapy, USA.
authors: R M
authors: RM
authors: Brightwell
authors: Roswell Park Cancer Institute, Department of Gynecologic Oncology, USA; Roswell Park Cancer Institute, Center for Immunotherapy, USA.
authors: K S
authors: KS
authors: Grzankowski
authors: Roswell Park Cancer Institute, Department of Gynecologic Oncology, USA.
authors: S
authors: S
authors: Lele
authors: Roswell Park Cancer Institute, Department of Biostatistics and Bioinformatics, USA.
authors: K
authors: K
authors: Eng
authors: Roswell Park Cancer Institute, Department of Pathology, USA.
authors: M
authors: M
authors: Arshad
authors: Roswell Park Cancer Institute, Department of Pathology, USA.
authors: H
authors: H
authors: Chen
authors: Roswell Park Cancer Institute, Department of Gynecologic Oncology, USA; Roswell Park Cancer Institute, Center for Immunotherapy, USA. Electronic address: kunle.odunsi@roswellpark.org.
authors: K
authors: K
authors: Odunsi
mesh_terms: D051928:CD47 Antigen / Q000032:analysis* / Q000235:genetics; D000077216:Carcinoma, Ovarian Epithelial; D005260:Female; D006801:Humans; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000737:chemistry*; D010051:Ovarian Neoplasms / Q000737:chemistry*; D046888:Tissue Array Analysis
chemical_list: D051928:CD47 Antigen; C497894:CD47 protein, human
keywords: CD4(+) T cells; CD47; CD8(+) T-cell activation; IFN-γ; NY-ESO-1; Ovarian cancer; SIRPα; anti-tumor effect