title: Impact of Tissue Sealing Sheet on Erectile Dysfunction in a Rat Model of Nerve-Sparing Radical Prostatectomy.
abstract: The tissue sealing sheet has recently been used to prevent intraoperative bleeding from the neurovascular bundles in radical prostatectomy. Surgical stress or inflammatory changes likely play a role in erectile dysfunction after cavernous nerve injury. However, the efficacy of a tissue sealing sheet for preventing erectile function after nerve-sparing radical prostatectomy remains unclear.
  
  
  
  To evaluate the effect of a tissue sealing sheet on erectile dysfunction after cavernous nerve dissection.
  
  
  
  Male Sprague-Dawley rats were randomly divided into three groups and subjected to sham operation or bilateral cavernous nerve dissection with (sheet group) or without (non-sheet group) a tissue sealing sheet. In the sheet group, cavernous nerves were sealed with a tissue sealing sheet immediately after cavernous nerve dissection.
  
  
  
  Erectile function was assessed by measuring intracavernous pressure and arterial pressure during pelvic nerve electrostimulation at 4 weeks after surgery. Expressions of interleukin-6, tumor growth factor-β1, and heme-oxygenase-1 in the major pelvic ganglion were examined by real-time polymerase chain reaction.
  
  
  
  Mean intracavernous pressure along with mean arterial pressure in the sheet group were similar to those in the sham group and showed a significant positive response compared with the non-sheet group (P < .05). Furthermore, expressions of interleukin-6, tumor growth factor-β1, and heme-oxygenase-1 were significantly lower in the sheet group than in the non-sheet group (P < .05).
  
  
  
  Use of a tissue sealing sheet attenuated postoperative inflammatory changes and oxidative stress and improved erectile function after cavernous nerve injury in rats. The tissue sealing sheet might become a useful therapeutic approach to preserve erectile function after nerve-sparing radical prostatectomy.
authors: Department of Urology, Tohoku University Graduate School of Medicine, Sendai, Japan. Electronic address: yamashita@uro.med.tohoku.ac.jp.
authors: Shinichi
authors: S
authors: Yamashita
authors: Department of Urology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Shinji
authors: S
authors: Fujii
authors: Department of Urology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Yoshihiro
authors: Y
authors: Kamiyama
authors: Department of Urology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Yoshihide
authors: Y
authors: Kawasaki
authors: Department of Urology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Hideaki
authors: H
authors: Izumi
authors: Department of Urology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Naoki
authors: N
authors: Kawamorita
authors: Department of Urology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Koji
authors: K
authors: Mitsuzuka
authors: Department of Urology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Hisanobu
authors: H
authors: Adachi
authors: Department of Urology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Yasuhiro
authors: Y
authors: Kaiho
authors: Department of Urology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Akihiro
authors: A
authors: Ito
authors: Department of Urology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Yoichi
authors: Y
authors: Arai
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007172:Erectile Dysfunction / Q000188:drug therapy / Q000209:etiology*; D006801:Humans; D007001:Hypogastric Plexus; D018189:Immunomagnetic Separation; D008297:Male; D010413:Penis / Q000294:innervation; D011468:Prostatectomy / Q000009:adverse effects* / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D020196:Trauma, Nervous System / Q000473:pathology
keywords: Cavernous Nerve Injury; Erectile Dysfunction; Nerve-Sparing Radical Prostatectomy; Tissue Sealing Sheet