title: Long noncoding RNA linc00598 regulates CCND2 transcription and modulates the G1 checkpoint.
abstract: Data derived from genomic and transcriptomic analyses have revealed that long noncoding RNAs (lncRNAs) have important roles in the transcriptional regulation of various genes. Recent studies have identified the mechanism underlying this function. To date, a variety of noncoding transcripts have been reported to function in conjunction with epigenetic regulator proteins. In this study, we investigated the function of linc00598, which is transcribed by a genomic sequence on chromosome 13, downstream of FoxO1 and upstream of COG6. Microarray analysis showed that linc00598 regulates the transcription of specific target genes, including those for cell cycle regulators. We discovered that linc00598 regulates CCND2 transcription through modulation of the transcriptional regulatory effect of FoxO1 on the CCND2 promoter. Moreover, we observed that knockdown of linc00598 induced G0/G1 cell cycle arrest and inhibited proliferation. These data indicate that linc00598 plays an important role in cell cycle regulation and proliferation through its ability to regulate the transcription of CCND2.
authors: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Oh-Seok
authors: OS
authors: Jeong
authors: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Yun-Cheol
authors: YC
authors: Chae
authors: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Hyeonsoo
authors: H
authors: Jung
authors: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Soon Cheol
authors: SC
authors: Park
authors: Department of Biology, College of Natural Sciences, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
authors: Sung-Jin
authors: SJ
authors: Cho
authors: Medical Research Center for Gene Regulation and Department of Pharmacology, Chonnam National University, Gwangju 501-746, Republic of Korea.
authors: Hyun
authors: H
authors: Kook
authors: Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: SangBeom
authors: S
authors: Seo
mesh_terms: D056742:Cyclin D2 / Q000096:biosynthesis* / Q000235:genetics; D000071161:Forkhead Box Protein O1 / Q000235:genetics / Q000378:metabolism; D059585:G1 Phase Cell Cycle Checkpoints / Q000502:physiology*; D057809:HEK293 Cells; D018922:HL-60 Cells; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D020014:K562 Cells; D061986:MCF-7 Cells; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D020218:Response Elements / Q000502:physiology*; D016192:Resting Phase, Cell Cycle / Q000502:physiology; D000074084:THP-1 Cells; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: C080336:CCND2 protein, human; D056742:Cyclin D2; C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D062085:RNA, Long Noncoding