title: Tetraspanin CD81 is an adverse prognostic marker in acute myeloid leukemia.
abstract: CD81 is a cell surface protein which belongs to the tetraspanin family. While in multiple myeloma its expression on plasma cells is associated with worse prognosis, this has not yet been explored in acute myeloid leukemia (AML). We measured membrane expression of CD81 on AML cells at diagnosis, evaluated its association with AML characteristics and its influence on patient outcome after intensive chemotherapy in a cohort of 134 patients. CD81 was detected in 92/134 (69%) patients. Patients with AML expressing CD81 had elevated leukocyte count (P=0.02) and were more likely classified as intermediate or adverse-risk by cytogenetics (P<0.001). CD81 expression had a negative impact on survival (event-free survival, overall survival and relapse-free survival) in univariate (P<0.001) and in multivariate analyses (P=0.003, 0.002 and <0.001, respectively). CD81 has a negative impact on OS in patients with NPM1 mutation (P=0.01) and in patients ELN-favorable (P=0.002). In conclusion, this cell surface marker may be a new prognostic marker for diagnostic risk classification and a potential therapeutic target for drug development in AML.
authors: Hematology Laboratory, Biology and Pathology Center, CHRU Lille, France.|Jean-Pierre AUBERT Research Center, UMR-S1172, Lille, France.
authors: Thomas
authors: T
authors: Boyer
authors: Jean-Pierre AUBERT Research Center, UMR-S1172, Lille, France.
authors: Soizic
authors: S
authors: Guihard
authors: Hematology Laboratory, Biology and Pathology Center, CHRU Lille, France.
authors: Christophe
authors: C
authors: Roumier
authors: Jean-Pierre AUBERT Research Center, UMR-S1172, Lille, France.
authors: Pauline
authors: P
authors: Peyrouze
authors: Jean-Pierre AUBERT Research Center, UMR-S1172, Lille, France.
authors: Fanny
authors: F
authors: Gonzales
authors: Jean-Pierre AUBERT Research Center, UMR-S1172, Lille, France.|Department of Hematology, Claude Huriez Hospital, CHRU Lille, France.
authors: Céline
authors: C
authors: Berthon
authors: Jean-Pierre AUBERT Research Center, UMR-S1172, Lille, France.|Department of Hematology, Claude Huriez Hospital, CHRU Lille, France.
authors: Bruno
authors: B
authors: Quesnel
authors: Hematology Laboratory, Biology and Pathology Center, CHRU Lille, France.|Jean-Pierre AUBERT Research Center, UMR-S1172, Lille, France.
authors: Claude
authors: C
authors: Preudhomme
authors: Department of Biostatistics, Univ. Lille, CHU Lille, EA 2694 - Santé Publique: Épidémiologie et Qualité des Soins, F-59000 Lille, France.
authors: Hélène
authors: H
authors: Behal
authors: Department of Biostatistics, Univ. Lille, CHU Lille, EA 2694 - Santé Publique: Épidémiologie et Qualité des Soins, F-59000 Lille, France.
authors: Alain
authors: A
authors: Duhamel
authors: Jean-Pierre AUBERT Research Center, UMR-S1172, Lille, France.|Institute of Medical Genetics, Jeanne de Flandre Hospital, CHRU Lille, France.
authors: Catherine
authors: C
authors: Roche-Lestienne
authors: Jean-Pierre AUBERT Research Center, UMR-S1172, Lille, France.
authors: Meyling
authors: M
authors: Cheok
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D018572:Disease-Free Survival; D005260:Female; D005434:Flow Cytometry; D005500:Follow-Up Studies; D042381:Granulocyte Precursor Cells / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D053208:Kaplan-Meier Estimate; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000235:genetics / Q000401:mortality* / Q000473:pathology*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics; D000090243:Nucleophosmin; D011379:Prognosis; D060225:Tetraspanin 28 / Q000378:metabolism*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C554798:CD81 protein, human; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D060225:Tetraspanin 28; D000090243:Nucleophosmin
keywords: CD81; acute myeloid leukemia; flow cytometry; prognosis; tetraspanin