title: Oxidative stress, genotoxicity and cytotoxicity of 1-methyl-3-octylimidazolium chloride on Paramisgurnus dabryanus.
abstract: This study evaluated the toxicity of 1-methyl-3-octylimidazolium chloride ([C8mim]Cl) on Paramisgurnus dabryanus by enzyme analysis, comet assay, and apoptosis analysis. The study showed that [C8mim]Cl had an obvious toxic effect inducing oxidative stress, genotoxicity, and cytotoxicity to fish liver cells. [C8mim]Cl also induced changes in the activities of superoxide dismutase and catalase, and the glutathione content and malondialdehyde level in fish exposed at 20-80mgL-1. With increased exposure concentration and time, the four antioxidant enzyme activities, three different comet parameters and apoptosis rates of tested cells were significantly increased, with significant differences (P<0.05 or P<0.01) observed between control group and each treatment group. This study shows that [C8mim]Cl could be a threat to aquatic organism health when accidentally released into aquatic ecosystems.
authors: Molecular and Genetic Laboratory, College of Life Science, Henan Normal University, No.46, East Jianshe Road, Xinxiang, Henan 453007, China. Electronic address: nanspring2002@163.com.
authors: Ping
authors: P
authors: Nan
authors: Molecular and Genetic Laboratory, College of Life Science, Henan Normal University, No.46, East Jianshe Road, Xinxiang, Henan 453007, China. Electronic address: yanshuaiguo@163.com.
authors: Shuai-Guo
authors: SG
authors: Yan
authors: Molecular and Genetic Laboratory, College of Life Science, Henan Normal University, No.46, East Jianshe Road, Xinxiang, Henan 453007, China. Electronic address: 1747973076@qq.com.
authors: Ya-Xing
authors: YX
authors: Wang
authors: Molecular and Genetic Laboratory, College of Life Science, Henan Normal University, No.46, East Jianshe Road, Xinxiang, Henan 453007, China. Electronic address: duqiyan09@tom.com.
authors: Qi-Yan
authors: QY
authors: Du
authors: Molecular and Genetic Laboratory, College of Life Science, Henan Normal University, No.46, East Jianshe Road, Xinxiang, Henan 453007, China. Electronic address: changzhongjie@tom.com.
authors: Zhong-Jie
authors: ZJ
authors: Chang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D020552:Comet Assay; D003531:Cypriniformes / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D054750:Ecotoxicology / Q000379:methods; D005978:Glutathione / Q000378:metabolism; D007093:Imidazoles / Q000008:administration & dosage / Q000633:toxicity*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009152:Mutagenicity Tests; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity
chemical_list: C586296:1-methyl-3-octylimidazolium chloride; D007093:Imidazoles; D014874:Water Pollutants, Chemical; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Antioxidant enzymes; Apoptosis; Comet assay; Paramisgurnus dabryanus; [C(8)mim]Cl