title: Morphological and functional alteration of erythrocyte ghosts and giant unilamellar vesicles caused by Vipera latifi venom.
abstract: Snake bites are an endemic public health problem in Iran, both in rural and urban area. Viper venom as a hemolytic biochemical "cocktail" of toxins, primarily cause to the systemic alteration of blood cells. In the sixties and seventies, human erythrocytes were extensively studied, but the mechanical and chemical stresses commonly exerted on red blood cells continue to attract interest of scientists for the study of membrane structure and function. Here, we monitor the effect of Vipera latifi venom on human erythrocytes ghost membranes using phase contrast and fluorescent microscopy and changes in ATPase activity under snake venom influence in vitro. The ion pumps [Na+,K+]-ATPase and (Ca2++Mg2+)-ATPase plays a pivotal role in the active transport of certain cations and maintenance of intracellular electrolyte homeostasis. We also describe the interaction of Vipera latifi (VL) venom with giant unilamellar vesicles (GUVs) composed of the native phospholipid mixtures visualized by the membrane fluorescence probe, ANS, used to assess the state of membrane and specifically mark the phospholipid domains.
authors: Orbeli Institute of Physiology, National Academy of Sciences of the Republic of Armenia, Orbely str. 22, 0019 Yerevan, Armenia.
authors: Gayane
authors: G
authors: Kirakosyan
authors: Orbeli Institute of Physiology, National Academy of Sciences of the Republic of Armenia, Orbely str. 22, 0019 Yerevan, Armenia.
authors: Maryam
authors: M
authors: Mohamadvarzi
authors: Orbeli Institute of Physiology, National Academy of Sciences of the Republic of Armenia, Orbely str. 22, 0019 Yerevan, Armenia.
authors: Lusine
authors: L
authors: Ghulikyan
authors: Orbeli Institute of Physiology, National Academy of Sciences of the Republic of Armenia, Orbely str. 22, 0019 Yerevan, Armenia.
authors: Naira
authors: N
authors: Zaqaryan
authors: Orbeli Institute of Physiology, National Academy of Sciences of the Republic of Armenia, Orbely str. 22, 0019 Yerevan, Armenia.
authors: Arsen
authors: A
authors: Kishmiryan
authors: Orbeli Institute of Physiology, National Academy of Sciences of the Republic of Armenia, Orbely str. 22, 0019 Yerevan, Armenia. Electronic address: taipan@ysu.am.
authors: Naira
authors: N
authors: Ayvazyan
mesh_terms: D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D006801:Humans; D007700:Kinetics; D008856:Microscopy, Fluorescence; D010743:Phospholipids / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D053835:Unilamellar Liposomes / Q000378:metabolism*; D014757:Viper Venoms / Q000633:toxicity*
chemical_list: D010743:Phospholipids; D053835:Unilamellar Liposomes; D014757:Viper Venoms; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Erythrocyte ghosts; GUVs; Vipera latifi; [Na(+),K(+)]-ATPase and (Ca(2+)+Mg(2+))-ATPases