title: Silk-Hydroxyapatite Nanoscale Scaffolds with Programmable Growth Factor Delivery for Bone Repair.
abstract: Osteoinductive biomaterials are attractive for repairing a variety of bone defects, and biomimetic strategies are useful toward developing bone scaffolds with such capacity. Here, a multiple biomimetic design was developed to improve the osteogenesis capacity of composite scaffolds consisting of hydroxyapatite nanoparticles (HA) and silk fibroin (SF). SF nanofibers and water-dispersible HA nanoparticles were blended to prepare the nanoscaled composite scaffolds with a uniform distribution of HA with a high HA content (40%), imitating the extracellular matrix (ECM) of bone. Bone morphogenetic protein-2 (BMP-2) was loaded in the SF scaffolds and HA to tune BMP-2 release. In vitro studies showed the preservation of BMP-2 bioactivity in the composite scaffolds, and programmable sustained release was achieved through adjusting the ratio of BMP-2 loaded on SF and HA. In vitro and in vivo osteogenesis studies demonstrated that the composite scaffolds showed improved osteogenesis capacity under suitable BMP-2 release conditions, significantly better than that of BMP-2 loaded SF-HA composite scaffolds reported previously. Therefore, these biomimetic SF-HA nanoscaled scaffolds with tunable BMP-2 delivery provide preferable microenvironments for bone regeneration.
authors: School of Biology and Basic Medical Sciences & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University , Suzhou 215123, People's Republic of China.
authors: Zhaozhao
authors: Z
authors: Ding
authors: Department of Orthopedics, The Second Affiliated Hospital of Soochow University , Suzhou 215000, People's Republic of China.
authors: Zhihai
authors: Z
authors: Fan
authors: National Engineering Laboratory for Modern Silk, Soochow University , Suzhou 215123, People's Republic of China.
authors: Xiaowei
authors: X
authors: Huang
authors: School of Biology and Basic Medical Sciences & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University , Suzhou 215123, People's Republic of China.|National Engineering Laboratory for Modern Silk, Soochow University , Suzhou 215123, People's Republic of China.
authors: Qiang
authors: Q
authors: Lu
authors: School of Biology and Basic Medical Sciences & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University , Suzhou 215123, People's Republic of China.
authors: Weian
authors: W
authors: Xu
authors: Department of Biomedical Engineering, Tufts University , Medford, Massachusetts 02155, United States.
authors: David L
authors: DL
authors: Kaplan
mesh_terms: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D001861:Bone Regeneration; D017886:Durapatite / Q000737:chemistry*; D010012:Osteogenesis; D047011:Silk; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D047011:Silk; D017886:Durapatite
keywords: biomimetic; bone repair; drug delivery; hydroxyapatite; silk