title: Development of Primer Pairs from Molecular Typing of Rabies Virus Variants Present in Mexico.
abstract: Nucleoprotein (N) gene from rabies virus (RABV) is a useful sequence target for variant studies. Several specific RABV variants have been characterized in different mammalian hosts such as skunk, dog, and bats by using anti-nucleocapsid monoclonal antibodies (MAbs) via indirect fluorescent antibody (IFA) test, a technique not available in many laboratories in Mexico. In the present study, a total of 158 sequences of N gene from RABV were used to design eight pairs of primers (four external and four internal primers), for typing four different RABV variants (dog, skunk, vampire bat, and nonhematophagous bat) which are most common in Mexico. The results indicate that the primer and the typing variant from the brain samples, submitted to nested and/or real-time PCR, are in agreement in all four singleplex reactions, and the designed primer pairs are an alternative for use in specific variant RABV typing.
authors: Laboratorio de Medicina Traslacional, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Santo Tomás, Miguel Hidalgo, 11340 Ciudad de México, CDMX, Mexico; Laboratorio de Biología Molecular, Laboratorio Estatal de Salud Pública del Estado de México, Paseo Tollocan s/n, La Moderna de la Cruz, 50180 Toluca, MEX, Mexico.
authors: Fernando
authors: 0000-0002-1670-5879
authors: F
authors: Bastida-González
authors: Laboratorio de Biología Molecular, Laboratorio Estatal de Salud Pública del Estado de México, Paseo Tollocan s/n, La Moderna de la Cruz, 50180 Toluca, MEX, Mexico.
authors: Dolores G
authors: DG
authors: Ramírez-Hernández
authors: Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Copilco Universidad, 04510 Coyoacán, CDMX, Mexico.
authors: Erika
authors: 0000-0002-1012-2760
authors: E
authors: Chavira-Suárez
authors: Laboratorio de Medicina Traslacional, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Santo Tomás, Miguel Hidalgo, 11340 Ciudad de México, CDMX, Mexico.
authors: Eleazar
authors: E
authors: Lara-Padilla
authors: Laboratorio de Medicina Traslacional, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Santo Tomás, Miguel Hidalgo, 11340 Ciudad de México, CDMX, Mexico.
authors: Paola
authors: P
authors: Zárate-Segura
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics*; D004285:Dogs; D060005:Genotyping Techniques / Q000379:methods*; D008800:Mexico; D019590:Nucleocapsid Proteins / Q000235:genetics*; D011820:Rabies virus / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D019590:Nucleocapsid Proteins; C099601:nucleocapsid protein, Rabies virus