title: Application of high-resolution melting analysis for differentiation of spoilage yeasts.
abstract: A new method based on high resolution melting (HRM) analysis was developed for the differentiation and classification of the yeast species that cause food spoilage. A total 134 strains belonging to 21 different yeast species were examined to evaluate the discriminative power of HRM analysis. Two different highly variable DNA regions on the 26 rRNA gene were targeted to produce the HRM profiles of each strain. HRM-based grouping was compared and confirmed by (GTG)5 rep-PCR fingerprinting analysis. All of the yeast species belonging to the genera Pichia, Candida, Kazachstania, Kluyveromyces, Debaryomyces, Dekkera, Saccharomyces, Torulaspora, Ustilago, and Yarrowia, which were produced as species-specific HRM profiles, allowed discrimination at species and/or strain level. The HRM analysis of both target regions provided successful discrimination that correlated with rep-PCR fingerprinting analysis. Consequently, the HRM analysis has the potential for use in the rapid and accurate classification and typing of yeast species isolated from different foods to determine their sources and routes as well as to prevent contamination.
authors: Faculty of Engineering, Food Engineering Department, Erciyes University, Kayseri, 38039, Turkey.
authors: Mine
authors: M
authors: Erdem
authors: Faculty of Engineering, Food Engineering Department, Erciyes University, Kayseri, 38039, Turkey. zkesmen@erciyes.edu.tr.
authors: Zülal
authors: Z
authors: Kesmen
authors: Faculty of Engineering, Food Engineering Department, Erciyes University, Kayseri, 38039, Turkey.
authors: Esra
authors: E
authors: Özbekar
authors: Faculty of Agriculture, Food Engineering Department, Ataturk University, Erzurum, 25240, Turkey.
authors: Bülent
authors: B
authors: Çetin
authors: Faculty of Engineering, Food Engineering Department, Erciyes University, Kayseri, 38039, Turkey.
authors: Hasan
authors: H
authors: Yetim
mesh_terms: D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics; D005516:Food Microbiology; D016533:Mycological Typing Techniques / Q000379:methods*; D010802:Phylogeny; D044366:Transition Temperature; D015003:Yeasts / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004271:DNA, Fungal
keywords: 26S rRNA gene; High-resolution melting analysis; melting curve; yeast species