title: miR-148a and miR-30a limit HCV-dependent suppression of the lipid droplet protein, ADRP, in HCV infected cell models.
abstract: Hepatitis C Virus (HCV) promotes lipid droplet (LD) formation and perturbs the expression of the LD associated PAT proteins ADRP and TIP47, to promote its own lifecycle. HCV enhances TIP47 and suppresses ADRP by displacing it from LD surface in infected cell models. We have previously shown that suppression of TIP47 by miR-148a and miR-30a decreased intracellular LDs and HCV RNA. Thus, this study aimed at examining whether this microRNA-mediated suppression of HCV would limit HCV-dependent displacement of ADRP from LDs. ADRP expression was examined in 21 HCV-infected liver biopsies and 9 healthy donor liver tissues as well as in HCV-infected Huh7 cells using qRT-PCR. miR-148a and miR-30a expression was manipulated using specific oligos in JFH-1 infected, oleic acid treated cells, to study their impact on ADRP expression using qRT-PCR, and immunofluorescence microscopy. Intracellular HCV RNA was assessed using qRT-PCR. ADRP is down regulated in patients as well as HCVcc-JFH-I infected cell models. Forcing the expression of both miRNAs induced ADRP on the mRNA and protein levels. This study shows that HCV suppresses hepatic ADRP expression in infected patients and cell lines. Forcing the expression of miR-148a and miR-30a limits the suppressive effect of HCV on ADRP. J. Med. Virol. 89:653-659, 2017. © 2016 Wiley Periodicals, Inc.
authors: Department of Pharmacology and Toxicology, Molecular Pathology Research Group, German University in Cairo, New Cairo City-Main Entrance Al Tagamoa Al Khames, Cairo, Egypt.|Scientific Affairs Unit, Egyptian Company for Biological Sciences, Giza, Egypt.
authors: Nada M
authors: NM
authors: El-Ekiaby
authors: Department of Pharmacology and Toxicology, Molecular Pathology Research Group, German University in Cairo, New Cairo City-Main Entrance Al Tagamoa Al Khames, Cairo, Egypt.|Faculty of Biotechnology, October University of Modern Sciences and Arts, Giza, Egypt.
authors: Radwa Y
authors: RY
authors: Mekky
authors: Department of Pharmacology and Toxicology, Molecular Pathology Research Group, German University in Cairo, New Cairo City-Main Entrance Al Tagamoa Al Khames, Cairo, Egypt.
authors: Sarah E
authors: SE
authors: Riad
authors: Department of Pharmacology and Toxicology, Molecular Pathology Research Group, German University in Cairo, New Cairo City-Main Entrance Al Tagamoa Al Khames, Cairo, Egypt.
authors: Dalia S
authors: DS
authors: Elhelw
authors: Endemic Medicine and Hepatology, Cairo University, Cairo, Egypt.
authors: Mohammed
authors: M
authors: El-Sayed
authors: Endemic Medicine and Hepatology, Cairo University, Cairo, Egypt.
authors: Gamal
authors: G
authors: Esmat
authors: Department of Pharmacology and Toxicology, Molecular Pathology Research Group, German University in Cairo, New Cairo City-Main Entrance Al Tagamoa Al Khames, Cairo, Egypt.|Biotechnology Graduate Program, American University in Cairo, AUC Avenue, New Cairo, Cairo, Egypt.
authors: Ahmed I
authors: AI
authors: Abdelaziz
mesh_terms: D000328:Adult; D001706:Biopsy; D002460:Cell Line; D005260:Female; D020869:Gene Expression Profiling; D016174:Hepacivirus / Q000472:pathogenicity*; D006526:Hepatitis C / Q000473:pathology; D022781:Hepatocytes / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D008099:Liver / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D000070780:Perilipin-2 / Q000037:antagonists & inhibitors*; D000070796:Perilipin-3 / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C550570:MIRN148 microRNA, human; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C000602982:PLIN2 protein, human; C038196:PLIN3 protein, human; D000070780:Perilipin-2; D000070796:Perilipin-3; D012367:RNA, Viral
keywords: PAT proteins; lipids; liver; microRNAs; viral infection