title: Synergistic effect of cellulase and xylanase during hydrolysis of natural lignocellulosic substrates.
abstract: Synergistic combination of cellulase and xylanase has been performed on pre-treated substrates in many previous studies, while few on natural substrates. In this study, three unpretreated lignocellulosic substrates were studied, including corncob, corn stover, and rice straw. The results indicated that when the mixed cellulase and xylanase were applied, reducing sugar concentrations were calculated as 19.53, 15.56, and 17.35mg/ml, respectively, based on the 3,5 dinitrosalicylic acid (DNS) method. Compared to the treatment with only cellulose, the hydrolysis yields caused by mixed cellulase and xylanase were improved by 133%, 164%, and 545%, respectively. In addition, the conversion yield of corncob, corn stover, and rice straw by cellulase-xylanase co-treatment reached 43.9%, 48.5%, and 40.2%, respectively, based on HPLC analysis, which confirmed the synergistic effect of cellulase-xylanase that was much higher than either of the single enzyme treatment. The substrate morphology was also evaluated to explore the synergistic mechanism of cellulase-xylanase.
authors: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China; College of Resources and Environmental Science, Hubei University, Wuhan 430062, PR China. Electronic address: htsong@hubu.edu.cn.
authors: Hui-Ting
authors: HT
authors: Song
authors: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: gaoyuan0808@sina.cn.
authors: Yuan
authors: Y
authors: Gao
authors: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: wahy-vv@163.com.
authors: Yi-Min
authors: YM
authors: Yang
authors: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: vickichiu1992@sina.com.
authors: Wen-Jing
authors: WJ
authors: Xiao
authors: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: liushihui1210@sina.com.
authors: Shi-Hui
authors: SH
authors: Liu
authors: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: 13035135871@163.com.
authors: Wu-Cheng
authors: WC
authors: Xia
authors: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: 1_zilulu@sina.com.
authors: Zi-Lu
authors: ZL
authors: Liu
authors: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: liyi@hubu.edu.cn.
authors: Li
authors: L
authors: Yi
authors: Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China; Hubei Key Laboratory of Industrial Biotechnology, College of Life Science, Hubei University, Wuhan 430062, PR China. Electronic address: zhbjiang@hubu.edu.cn.
authors: Zheng-Bing
authors: ZB
authors: Jiang
mesh_terms: D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D012275:Oryza / Q000737:chemistry* / Q000378:metabolism; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D014995:Xylosidases / Q000737:chemistry* / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002241:Carbohydrates; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D014995:Xylosidases; D002480:Cellulase
keywords: Cellulose; Corn stover; Corncob; Rice straw; Synergistic effect; Xylanase