title: Quantifying bisphenol A in maternal and cord whole blood using isotope dilution liquid chromatography/tandem mass spectrometry and maternal characteristics associated with bisphenol A.
abstract: Bisphenol A (BPA) is endocrine disrupting chemical that have been detected among general population. Exposure levels among pregnant women and their fetus are yet largely unknown among Japanese. We have developed a new method of measuring total BPA in whole blood samples by using isotopic dilution liquid chromatography-tandem mass spectrometry (ID-LC/MS/MS). For eliminating possible contaminations, we have used glass cartridge instead of polypropylene cartridge and successfully reduced background levels. Additionally gap retention technique was applied to improve sensitivity. We also confirmed no external contamination by measuring free BPA in the samples. The limit of quantification (LOQ) was 0.040 ng/ml. With this developed method, we determined total BPA concentrations of 59 maternal blood at delivery and 285 cord blood samples in prospective birth cohort study and investigated factors possibly related to total BPA levels. Total BPA levels ranged from below LOQ to 0.419 ng/ml and for maternal blood and from below LOQ to 0.217 ng/ml for cord blood, respectively. The geometric mean was 0.051 ng/ml for maternal blood and 0.046 ng/ml for cord blood, respectively. Although no correlation was observed between maternal and fetal blood levels of total BPA, our result suggested fetal exposure to BPA. We have found that younger mothers, frequent beef and pork consumption during pregnancy were positively associated with maternal total BPA levels. We confirmed in utero exposure to BPA, which highlights the importance of further studies of investing the effects of fetus BPA exposure on health outcomes.
authors: Institute of Environmental Ecology, Idea Consultants, Inc., 1334-5 Riemon, Yaizu, Shizuoka, 421-0212, Japan.
authors: Jun
authors: J
authors: Yamamoto
authors: Center for Environmental and Health Sciences, Hokkaido University, Kita 12, Nishi 7, Kita-ku, Sapporo, 060-0812, Japan.
authors: Machiko
authors: M
authors: Minatoya
authors: Department of Public Health, Hokkaido University Graduate School of Medicine, Hokkaido University, Kita 15, Nishi 7, Kita-ku, Sapporo, 060-8638, Japan.
authors: Seiko
authors: S
authors: Sasaki
authors: Center for Environmental and Health Sciences, Hokkaido University, Kita 12, Nishi 7, Kita-ku, Sapporo, 060-0812, Japan.
authors: Atsuko
authors: A
authors: Araki
authors: Center for Environmental and Health Sciences, Hokkaido University, Kita 12, Nishi 7, Kita-ku, Sapporo, 060-0812, Japan.
authors: Chihiro
authors: C
authors: Miyashita
authors: Institute of Environmental Ecology, Idea Consultants, Inc., 1334-5 Riemon, Yaizu, Shizuoka, 421-0212, Japan.
authors: Toru
authors: T
authors: Matsumura
authors: Center for Environmental and Health Sciences, Hokkaido University, Kita 12, Nishi 7, Kita-ku, Sapporo, 060-0812, Japan. Electronic address: rkishi@med.hokudai.ac.jp.
authors: Reiko
authors: R
authors: Kishi
mesh_terms: D000328:Adult; D000818:Animals; D001559:Benzhydryl Compounds / Q000097:blood*; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D052244:Endocrine Disruptors / Q000097:blood*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D005518:Food Preferences; D006801:Humans; D057230:Limit of Detection; D018811:Maternal Exposure; D008431:Maternal-Fetal Exchange; D010636:Phenols / Q000097:blood*; D011247:Pregnancy; D011446:Prospective Studies; D000069466:Red Meat / Q000032:analysis; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Cord blood; Human exposure; Japan; Maternal blood; Pregnant women