title: SPECT/CT analysis of splenic function in genistein-treated malaria-infected mice.
abstract: Spleen traps malaria-infected red blood cells, thereby leading to splenomegaly. Splenomegaly induces impairment in splenic function, i.e., rupture. Therefore, splenomegaly inhibition is required to protect the spleen. In our previous study, genistein was found to have an influence on malaria-induced splenomegaly. However, the effect of genistein in malaria-induced splenomegaly, especially on the function of spleen, has not been fully investigated. In this study, hematoxylin and eosin (H&E) staining images show that genistein partially prevents malaria-induced architectural disruption of spleen. In addition, genistein decreases transgenic Plasmodium parasites accumulation in the spleen. Genistein treatment can protect splenic function from impairment caused by malaria infection. To examine the functions of malaria-infected spleen, we employed single-photon emission computed tomography/computed tomography (SPECT/CT) technology. Red blood cells are specifically radiolabeled with Technetium-99m pertechnetate (99mTcO4-) and trapped inside the spleen. The standardized uptake values (SUVs) in the spleen of infected mice are higher than those of naive and genistein-treated mice. However, genistein reduces the malaria-induced trapping capacity of spleen for heat-damaged radiolabeled RBCs, while exhibiting a protective effect against malaria. Considering these results, we suggested that genistein could be effectively used in combination therapy for malaria-induced splenic impairment.
authors: Division of Integrative Bioscience and Bioengineering, Center for Biofluid and Biomimic Research, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, 790-784, Republic of Korea.
authors: Young Ran
authors: YR
authors: Ha
authors: Pohang Center for Evaluation of Biomaterials, Pohang Technopark, Pohang, Gyeongbuk, 790-834, Republic of Korea.
authors: Sung-A
authors: SA
authors: Kang
authors: Department of Mechanical Engineering, Center for Biofluid and Biomimic Research, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, 790-784, Republic of Korea.
authors: Jeongeun
authors: J
authors: Ryu
authors: School of Mechanical Engineering, Pusan National University, Pusan, 609-735, Republic of Korea.
authors: Eunseop
authors: E
authors: Yeom
authors: Pohang Center for Evaluation of Biomaterials, Pohang Technopark, Pohang, Gyeongbuk, 790-834, Republic of Korea.
authors: Mun Ki
authors: MK
authors: Kim
authors: Department of Mechanical Engineering, Center for Biofluid and Biomimic Research, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, 790-784, Republic of Korea. Electronic address: sjlee@postech.ac.kr.
authors: Sang Joon
authors: SJ
authors: Lee
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000473:pathology; D019833:Genistein / Q000494:pharmacology / Q000627:therapeutic use*; D006358:Hot Temperature / Q000009:adverse effects; D008156:Luciferases / Q000378:metabolism; D008163:Luminescent Measurements; D008288:Malaria / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010962:Plasmodium berghei / Q000201:enzymology / Q000302:isolation & purification; D047428:Protein Kinase Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D000072098:Single Photon Emission Computed Tomography Computed Tomography; D013154:Spleen / Q000000981:diagnostic imaging / Q000187:drug effects / Q000503:physiopathology*; D013163:Splenomegaly / Q000188:drug therapy / Q000469:parasitology; D014463:Ultrasonography
chemical_list: D047428:Protein Kinase Inhibitors; D019833:Genistein; D008156:Luciferases
keywords: Genistein; Plasmodium berghei; SPECT/CT; Splenomegaly