title: Mild hypothermia facilitates the long-term survival of newborn cells in the dentate gyrus after traumatic brain injury by diminishing a pro-apoptotic microenvironment.
abstract: Although previous research has demonstrated that traumatic brain injury (TBI) accelerates the proliferation of neural stem cells in dentate gyrus of the hippocampus, most of these newborn cells undergo apoptosis in a traumatic microenvironment. Thus, promoting the long-term survival of newborn cells during neurogenesis is a compelling goal for the treatment of TBI. In this study, we investigated whether mild hypothermia (MHT) therapy, which mitigates the multiple secondary injury cascades of TBI, enhances the survival of newborn cells. SD rats were subjected to unilateral fluid percussion injury and received MHT therapy for 4h (33.5°C). Bromodeoxyuridine (BrdU) was administered to label the mitotic cells. Spatial learning and memory were evaluated with the Morris water maze test. Brain sections were immunostained with antibodies against BrdU, DCX (a neuroblast marker) or NeuN (a mature neuron marker). The apoptosis levels in the dentate gyrus were examined with antibodies against the apoptotic proteins FAS, FASL, Bcl-2 and cleaved caspase 3. The results indicated that MHT could significantly prevent TBI-induced cognitive impairments. At 1week after injury, the density of BrdU-immunoreactive cells significantly increased in both TBI and TBI+MHT rats. At 4weeks after injury, the density of BrdU-positive cells further increased in TBI+MHT rats, whereas the density declined in the TBI rats. The density of DCX-positive cells in SGZ of the hippocampus at 1week after injury in the TBI+MHT rats was significantly greater than in the TBI rats. Moreover, the density of NeuN-positive cells in the subgranular zone at 4weeks after injury and in the granule cell layer at 7weeks after injury was significantly increased in the TBI+MHT rats. The TBI+MHT rats displayed a lower level of apoptosis in the dentate gyrus compared with the TBI rats. These data indicate that TBI could only facilitate a burst of proliferation and short-term survival of newborn cells, whereas TBI+MHT could facilitate long-term survival and maturation of newborn cells through diminishing pro-apoptotic microenvironment. These results suggest that MHT-mediated neurogenesis may have an important therapeutic potential for the endogenous repair of TBI.
authors: Institute of Traumatic Brain Injury and Neurology, Pingjin Hospital, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China; Key Laboratory of Neurotrauma Repair of Tianjin, Tianjin 300162, China.
authors: Chong
authors: C
authors: Chen
authors: Institute of Traumatic Brain Injury and Neurology, Pingjin Hospital, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China; Key Laboratory of Neurotrauma Repair of Tianjin, Tianjin 300162, China.
authors: Tie-Zhu
authors: TZ
authors: Ma
authors: Institute of Traumatic Brain Injury and Neurology, Pingjin Hospital, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China; Key Laboratory of Neurotrauma Repair of Tianjin, Tianjin 300162, China.
authors: Li-Na
authors: LN
authors: Wang
authors: Institute of Traumatic Brain Injury and Neurology, Pingjin Hospital, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China; Key Laboratory of Neurotrauma Repair of Tianjin, Tianjin 300162, China.
authors: Jing-Jing
authors: JJ
authors: Wang
authors: Institute of Traumatic Brain Injury and Neurology, Pingjin Hospital, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China; Key Laboratory of Neurotrauma Repair of Tianjin, Tianjin 300162, China.
authors: Yue
authors: Y
authors: Tu
authors: Institute of Traumatic Brain Injury and Neurology, Pingjin Hospital, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China; Key Laboratory of Neurotrauma Repair of Tianjin, Tianjin 300162, China.
authors: Ming-Liang
authors: ML
authors: Zhao
authors: Institute of Traumatic Brain Injury and Neurology, Pingjin Hospital, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China; Key Laboratory of Neurotrauma Repair of Tianjin, Tianjin 300162, China.
authors: Sai
authors: S
authors: Zhang
authors: Institute of Traumatic Brain Injury and Neurology, Pingjin Hospital, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China; Key Laboratory of Neurotrauma Repair of Tianjin, Tianjin 300162, China. Electronic address: chenmo333@163.com.
authors: Hong-Tao
authors: HT
authors: Sun
authors: Institute of Traumatic Brain Injury and Neurology, Pingjin Hospital, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China; Key Laboratory of Neurotrauma Repair of Tianjin, Tianjin 300162, China. Electronic address: lixiaohong12@hotmail.com.
authors: Xiao-Hong
authors: XH
authors: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D000070642:Brain Injuries, Traumatic / Q000473:pathology*; D001973:Bromodeoxyuridine / Q000378:metabolism; D060833:Cellular Microenvironment / Q000502:physiology*; D018891:Dentate Gyrus / Q000473:pathology*; D000089662:Doublecortin Protein; D008297:Male; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology
chemical_list: C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D001973:Bromodeoxyuridine
keywords: apoptosis; mild hypothermia; neurogenesis; survival; traumatic brain injury