title: Comparative metabolic and lipidomic profiling of human breast cancer cells with different metastatic potentials.
abstract: This study conducted comprehensive and comparative metabolic and lipidomic profiling of a human epithelial breast cell line (MCF-10A), a slightly metastatic (MCF-7), and a highly metastatic (MDA-MB-231) breast cancer cell line using gas chromatography mass spectrometry (GC-MS) and direct infusion mass spectrometry (DI-MS). Among 39 metabolites identified by GC-MS analysis, xanthine, glucose-6-phosphate, mannose-6-phosphate, guanine, and adenine were selected as prognostic markers of breast cancer metastasis. Major metabolic pathways involved in differentiation of the cell lines were alanine, aspartate, and glutamate metabolism, purine metabolism and glycine, serine, and threonine metabolism. Among 44 intact lipid species identified by DI-MS analysis, the levels of most phospholipids were higher in both metastatic groups than in normal cells. Specifically, the levels of phosphatidylserine (PS) 18:0/20:4, phosphatidylinositol (PI) 18:0/20:4, and phosphatidylcholine (PC) 18:0/20:4 were markedly higher while those of phosphatidylethanolamine (PE) 18:1/18:1 and PI 18:0/18:1 were lower in MDA-MB-231 cells than in MCF-7 cells. A partial-least-squares regression model was developed and validated for predicting the metastatic potential of breast cancer cells. The information obtained in this study will be useful when developing diagnostic tools and for identifying potential therapeutic targets for metastatic breast cancer.
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Hye-Youn
authors: HY
authors: Kim
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Kyung-Min
authors: KM
authors: Lee
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: So-Hyun
authors: SH
authors: Kim
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Yeo-Jung
authors: YJ
authors: Kwon
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Young-Jin
authors: YJ
authors: Chun
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Hyung-Kyoon
authors: HK
authors: Choi
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008055:Lipids / Q000032:analysis*; D055442:Metabolome
chemical_list: D008055:Lipids
keywords: PLSR; breast cancer cells; lipidomics; metabolomics; metastasis