title: Effect of phosphorus deficiency on erythrocytic morphology and function in cows.
abstract: The aim of this study was to evaluate the influence of phosphorus (P) deficiency on the morphological and functional characteristics of erythrocytes in cows. Forty Holstein-Friesian dairy cows in mid-lactation were randomly divided into two groups of 20 each and were fed either a low-P diet (0.03% P/kg dry matter [DM]) or a control diet (0.36% P/kg DM). Red blood cell (RBC) indices results showed RBC and mean corpuscular hemoglobin decreased while mean corpuscular volume increased significantly (p＜ 0.05) in P-deficient cows. Erythrocyte morphology showed erythrocyte destruction in P-deficient cows. Erythrocytes' functional characteristics results showed total bilirubin and indirect bilirubin concentrations and aspartate transaminase and alanine transaminase activity levels in the serum of P-deficient cows were significantly higher than those in control diet-fed cows. Activities of superoxide dismutase and glutathione peroxidase in erythrocytes were lower, while the malondialdehyde content was greater, in P-deficient cows than in control diet-fed cows. Na+/K+-ATPase and Mg2+-ATPase activities were lower in P-deficient cows than in control diet-fed cows; however, Ca2+-ATPase activity was not significantly different. The phospholipid composition of the erythrocyte membrane changed and membrane fluidity rigidified in P-deficient cows. The results indicate that P deficiency might impair erythrocyte integrity and functional characteristics in cows.
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Ziwei
authors: Z
authors: Zhang
authors: Harbin Railway Public Security Bureau police dog base, Harbin 150056, China.
authors: Mingyu
authors: M
authors: Bi
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Jie
authors: J
authors: Yang
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Haidong
authors: H
authors: Yao
authors: College of Life Science, Northeast Agricultural University, Harbin 150030, China.
authors: Zhonghua
authors: Z
authors: Liu
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Shiwen
authors: S
authors: Xu
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D002417:Cattle / Q000097:blood; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000473:pathology* / Q000502:physiology; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D010758:Phosphorus / Q000172:deficiency*; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D010758:Phosphorus; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000254:Sodium-Potassium-Exchanging ATPase; D001663:Bilirubin
keywords: cow; erythrocyte; function; morphology; phosphorus deficiency