title: Influence of the graft density of hydrophobic groups on thermo-responsive nanoparticles for anti-cancer drugs delivery.
abstract: A series of deoxycholate-chitosan-hydroxybutyl (DAHBCs) with different degrees of substitution (DS) of hydrophobic deoxycholate (DOCA) were successfully synthesized. The lower critical solution temperature (LCST) of various DAHBCs could be adjusted from 35.4°C to 42.1°C by controlling the graft density of DOCA. DAHBCs could self-assemble into nanoparticles (NPs) which gradually evolved from irregular aggregates to spherical particles with the decrease of the DS of DOCA groups. The size of DAHBCs NPs ranged from 100nm to 250nm and their zeta potential varied between 3.85 and 12.37mV. Hemolysis tests and protein adsorption assay exhibited DAHBCs NPs had few adverse effects on the blood components even at a concentration as high as 1mg/mL. DAHBCs NPs showed high curcumin (CUR) encapsulation efficiency up to 80%. CUR-loaded DAHBCs NPs displayed thermal-dependent drug release profiles, and the release rate of CUR (∼75%) was significantly (p<0.05) accelerated at a temperature above the LCST compared with that (∼40%) below the LCST. Cytotoxicity analysis identified no toxicity associated with DAHBCs NPs at a concentration up to 0.5mg/mL. However, when the cells were incubated with the CUR-loaded NPs, their growth was significantly inhibited at 43°C (>LCST), demonstrating the thermal-responsive release of encapsulated cargoes from the NPs. With the capacity to control the LCST of DAHBCs NPs at specific temperatures, it could be speculated that DAHBCs NPs might serve as a promising thermo-responsive nanoplatform for the delivery of antitumor drugs.
authors: College of Marine Life Science, Ocean University of China, 5(#) Yushan Road, Qingdao 266003, China.
authors: Fangqin
authors: F
authors: Wang
authors: College of Marine Life Science, Ocean University of China, 5(#) Yushan Road, Qingdao 266003, China.
authors: Guixue
authors: G
authors: Xia
authors: College of Marine Life Science, Ocean University of China, 5(#) Yushan Road, Qingdao 266003, China.
authors: Xuqian
authors: X
authors: Lang
authors: College of Marine Life Science, Ocean University of China, 5(#) Yushan Road, Qingdao 266003, China.
authors: Xuanxuan
authors: X
authors: Wang
authors: College of Marine Life Science, Ocean University of China, 5(#) Yushan Road, Qingdao 266003, China.
authors: Zixian
authors: Z
authors: Bao
authors: College of Marine Life Science, Ocean University of China, 5(#) Yushan Road, Qingdao 266003, China.
authors: Zeenat
authors: Z
authors: Shah
authors: College of Marine Life Science, Ocean University of China, 5(#) Yushan Road, Qingdao 266003, China.
authors: Xiaojie
authors: X
authors: Cheng
authors: College of Marine Life Science, Ocean University of China, 5(#) Yushan Road, Qingdao 266003, China.
authors: Ming
authors: M
authors: Kong
authors: College of Marine Life Science, Ocean University of China, 5(#) Yushan Road, Qingdao 266003, China.
authors: Chao
authors: C
authors: Feng
authors: College of Marine Life Science, Ocean University of China, 5(#) Yushan Road, Qingdao 266003, China. Electronic address: yaliu@ouc.edu.cn.
authors: Ya
authors: Y
authors: Liu
authors: College of Marine Life Science, Ocean University of China, 5(#) Yushan Road, Qingdao 266003, China. Electronic address: xgchen@ouc.edu.cn.
authors: Xiguang
authors: X
authors: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage*; D001672:Biocompatible Materials; D016503:Drug Delivery Systems; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials
keywords: Drug delivery; Graft density; Hydrophobic group; Nanoparticles; Thermo-responsive