title: Salvianolic acid B induced upregulation of miR-30a protects cardiac myocytes from ischemia/reperfusion injury.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are a novel class of powerful, endogenous regulators of gene expression. This study was designed to ascertain if miR-30a is involved in the cardioprotective actions of salvianolic acid B (Sal B) against myocardial ischemia-reperfusion (I-R) injury through suppression of autophagy.
  
  
  METHODS
  Murine myocardial cells that had undergone primary culture were induced by I-R and incubated with Sal B (25, 50, 100 μM) in the presence of a miR-30a mimic or miR-30a inhibitor. Expression of miR-30a, beclin-1, LC3-II and p-Akt protein, cell viability, and lactic acid dehydrogenase (LDH) release were assessed.
  
  
  RESULTS
  miR-30a expression was down-regulated remarkably in I-R cells, and this suppression could be reversed by Sal B in a dose-dependent manner. Sal B repressed autophagy in I-R myocardial cells. Sal B improved cell viability and reduced the rate of LDH leakage, which suggested that autophagy suppression was beneficial for cell survival. Knockdown of miR-30a with a miR-30a inhibitor could reverse the anti-autophagy effect of Sal B against I-R injury. Furthermore, we confirmed that Sal B has a protective role in miR-30a-mediated autophagy through the PI3K/Akt signaling pathway, which was abrogated by the PI3K inhibitor LY294002.
  
  
  CONCLUSIONS
  These data suggest that miR-30a is involved in Sal B-mediated cardioprotection against I-R injury through the PI3K/Akt signaling pathway.
authors: Beijing Key Lab of Chinese Materia Medica Quality Evaluation, School of Chinese Pharmacy, Beijing University of Chinese Medicine, 6 Wngjing Zhonghuan Nanlu, Chaoyang District, Beijing, 100102, China.
authors: Dan
authors: D
authors: Li
authors: Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
authors: Jun
authors: J
authors: Wang
authors: Institute of Basic Medical Sciences of Xiyuan Hospital, Beijing Key Laboratory of Pharmacology of Chinese Materia Medicine, Beijing, 100091, China.
authors: Jincai
authors: J
authors: Hou
authors: Institute of Basic Medical Sciences of Xiyuan Hospital, Beijing Key Laboratory of Pharmacology of Chinese Materia Medicine, Beijing, 100091, China.
authors: Jianhua
authors: J
authors: Fu
authors: Institute of Basic Medical Sciences of Xiyuan Hospital, Beijing Key Laboratory of Pharmacology of Chinese Materia Medicine, Beijing, 100091, China. ljxunljxun@163.com.
authors: Jianxun
authors: J
authors: Liu
authors: Beijing Key Lab of Chinese Materia Medica Quality Evaluation, School of Chinese Pharmacy, Beijing University of Chinese Medicine, 6 Wngjing Zhonghuan Nanlu, Chaoyang District, Beijing, 100102, China. linrch307@sina.com.
authors: Ruichao
authors: R
authors: Lin
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects; D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects*; D053630:Depsides
chemical_list: D001572:Benzofurans; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; C076944:salvianolic acid B; D053630:Depsides
keywords: Autophagy; Ischemic heart disease; Salvianolic acid B; miR-30a