title: Early developmental bisphenol-A exposure sex-independently impairs spatial memory by remodeling hippocampal dendritic architecture and synaptic transmission in rats.
abstract: Bisphenol-A (BPA, 4, 4'-isopropylidene-2-diphenol), a synthetic xenoestrogen that widely used in the production of polycarbonate plastics, has been reported to impair hippocampal development and function. Our previous study has shown that BPA exposure impairs Sprague-Dawley (SD) male hippocampal dendritic spine outgrowth. In this study, the sex-effect of chronic BPA exposure on spatial memory in SD male and female rats and the related synaptic mechanism were further investigated. We found that chronic BPA exposure impaired spatial memory in both SD male and female rats, suggesting a dysfunction of hippocampus without gender-specific effect. Further investigation indicated that BPA exposure causes significant impairment of dendrite and spine structure, manifested as decreased dendritic complexity, dendritic spine density and percentage of mushroom shaped spines in hippocampal CA1 and dentate gyrus (DG) neurons. Furthermore, a significant reduction in Arc expression was detected upon BPA exposure. Strikingly, BPA exposure significantly increased the mIPSC amplitude without altering the mEPSC amplitude or frequency, accompanied by increased GABAARβ2/3 on postsynaptic membrane in cultured CA1 neurons. In summary, our study indicated that Arc, together with the increased surface GABAARβ2/3, contributed to BPA induced spatial memory deficits, providing a novel molecular basis for BPA achieved brain impairment.
authors: College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China.
authors: Zhi-Hua
authors: ZH
authors: Liu
authors: College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China.
authors: Jin-Jun
authors: JJ
authors: Ding
authors: College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China.
authors: Qian-Qian
authors: QQ
authors: Yang
authors: College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China.
authors: Hua-Zeng
authors: HZ
authors: Song
authors: School of Pharmacy, Anhui Medical University, Hefei 230038, PR China.
authors: Xiang-Tao
authors: XT
authors: Chen
authors: College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China.
authors: Yi
authors: Y
authors: Xu
authors: College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China.
authors: Gui-Ran
authors: GR
authors: Xiao
authors: College of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China.
authors: Hui-Li
authors: HL
authors: Wang
mesh_terms: D000284:Administration, Oral; D000395:Air Pollutants, Occupational / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D049229:Dendritic Spines / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D018891:Dentate Gyrus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005786:Gene Expression Regulation; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009473:Neuronal Plasticity / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D061251:Primary Cell Culture; D017966:Pyramidal Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000235:genetics / Q000378:metabolism; D065852:Spatial Memory / Q000187:drug effects* / Q000502:physiology; D013569:Synapses / Q000187:drug effects / Q000502:physiology; D055366:Synaptic Potentials / Q000187:drug effects; D009435:Synaptic Transmission / Q000187:drug effects; D013702:Temporal Lobe / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D000098826:Bisphenol A Compounds
chemical_list: D000395:Air Pollutants, Occupational; D001559:Benzhydryl Compounds; D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins; D010636:Phenols; D011963:Receptors, GABA-A; C092069:activity regulated cytoskeletal-associated protein; C006780:bisphenol A; D000098826:Bisphenol A Compounds