title: Current molecular profile of juvenile nasopharyngeal angiofibroma: First comprehensive study from India.
abstract: An attempt is made to analyze the molecular behavior of juvenile nasopharyngeal angiofibroma (JNA).
  
  
  
  Case Series METHODS: Quantification of mRNAs expression was undertaken through real-time polymerase chain reaction in JNA (9-24) samples for VEGF-A, basic fibroblast growth factor (b-FGF), platelet-derived growth factor PDGF-A, KIT proto-oncogene receptor tyrosine kinase (c-Kit), Avian myelomatosis viral oncogene homolog (c-Myc), Harvey rat sarcoma viral oncogene homolog (H-Ras), tumor suppressor gene TP53, and androgen receptor and interleukin 6 (IL-6). The β-catenin expression was evaluated by western blot in 16 samples. Nasal polyp was taken as control.
  
  
  
  A significantly increased (P < 0.01) expression of c-myc, VEGFA, bFGF, PDGFA, c-kit, and TP53 was seen, along with enhanced expression of β-catenin. A massive enhancement of H-Ras expression was seen for the first time. Androgen receptor expression was no different, whereas IL-6 despite showing upregulation trend was not significant.
  
  
  
  The enhanced expressions of various markers suggest their potential role in JNA. Although the biological significance of c-kit, c-myc, and one of the novel markers H-Ras has yet to be defined, their significant expression may have a therapeutic importance.
  
  
  
  NA. Laryngoscope, 127:E100-E106, 2017.
authors: Biochemistry and Endocrinology Divisions, Central Drug Research Institute, Lucknow, India.
authors: Praveen
authors: P
authors: Pandey
authors: Department of Otorhinolaryngology, King George Medical University, Lucknow, India.
authors: Anupam
authors: A
authors: Mishra
authors: Department of Otorhinolaryngology, King George Medical University, Lucknow, India.
authors: Ashoak Mani
authors: AM
authors: Tripathi
authors: Department of Otorhinolaryngology, King George Medical University, Lucknow, India.
authors: Veerendra
authors: V
authors: Verma
authors: Biochemistry and Endocrinology Divisions, Central Drug Research Institute, Lucknow, India.
authors: Ritu
authors: R
authors: Trivedi
authors: Department of Otorhinolaryngology, King George Medical University, Lucknow, India.
authors: Hitendra Prakash
authors: HP
authors: Singh
authors: Department of Otorhinolaryngology, King George Medical University, Lucknow, India.
authors: Sunil
authors: S
authors: Kumar
authors: Department of Otorhinolaryngology, King George Medical University, Lucknow, India.
authors: Brijesh
authors: B
authors: Patel
authors: Department of Otorhinolaryngology, King George Medical University, Lucknow, India.
authors: Vinay
authors: V
authors: Singh
authors: Department of Biochemistry, King George Medical University, Lucknow, India.
authors: Shivani
authors: S
authors: Pandey
authors: Department of Clinical Genetics, King George Medical University, Lucknow, India.
authors: Amita
authors: A
authors: Pandey
authors: Department of Otolaryngology, Nepalgunj Medical College, Nepal.
authors: Subhash Chandra
authors: SC
authors: Mishra
mesh_terms: D000293:Adolescent; D018322:Angiofibroma / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology*; D001707:Biopsy, Needle; D002648:Child; D015331:Cohort Studies; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D005819:Genetic Markers; D055106:Genome-Wide Association Study; D006801:Humans; D007150:Immunohistochemistry; D007194:India / Q000453:epidemiology; D008297:Male; D009303:Nasopharyngeal Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology*; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: C410798:AR protein, human; D005819:Genetic Markers; C000715470:MAS1 protein, human; D010982:Platelet-Derived Growth Factor; D000090063:Proto-Oncogene Mas; D011944:Receptors, Androgen; C075278:platelet-derived growth factor A; D016222:Fibroblast Growth Factor 2
keywords: AR; FGF; IL-6; Juvenile nasopharyngeal angiofibroma; PDGF; Ras; VEGF; beta catenin; c-Kit; c-Myc; p53