title: Enhanced conductivity of rGO/Ag NPs composites for electrochemical immunoassay of prostate-specific antigen.
abstract: Electrode materials play a vital role in the development of electrochemical immunosensors (EIs), particularly of label-free EIs. In this study, composites containing reduced graphene oxide with silver nanoparticles (rGO/Ag NPs) were synthesized using binary reductants, i.e. hydrazine hydrate and sodium citrate. Due to the fact that graphene oxide (GO) was fully restored to rGO, and rGO stacking was effectively inhibited by insertion of small Ag NPs between the graphene sheets, the electrical conductivity of rGO/Ag NPs composites was significantly improved compared to rGO alone, with an enhancement factor of 346% at 40wt% of rGO. Moreover, the conducting path between rGO and Ag NPs formed because the structural defects in rGO were effectively repaired by decoration with Ag NPs. Subsequently, based on a screen-printed three-electrode system, a label-free EI for detecting prostate-specific antigen (PSA) was constructed using rGO/Ag NPs composites as a support material. The fabricated EIs demonstrated a wide linear response range (1.0-1000ng/ml), low detection limit (0.01ng/ml) and excellent specificity, reproducibility and stability. Thus, the proposed EIs based on rGO/Ag NPs composites can be easily extended for the ultrasensitive detection of different protein biomarkers.
authors: Beijing Engineering Research Center of Printed Electronics, Beijing Institute of Graphic Communication, Beijing, 102600 PR China.
authors: Lu
authors: L
authors: Han
authors: Beijing Engineering Research Center of Printed Electronics, Beijing Institute of Graphic Communication, Beijing, 102600 PR China.
authors: Cheng-Mei
authors: CM
authors: Liu
authors: Beijing Engineering Research Center of Printed Electronics, Beijing Institute of Graphic Communication, Beijing, 102600 PR China.
authors: Shi-Lei
authors: SL
authors: Dong
authors: Beijing Engineering Research Center of Printed Electronics, Beijing Institute of Graphic Communication, Beijing, 102600 PR China.
authors: Cai-Xia
authors: CX
authors: Du
authors: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing, 100084 PR China.
authors: Xiao-Yong
authors: XY
authors: Zhang
authors: Beijing Engineering Research Center of Printed Electronics, Beijing Institute of Graphic Communication, Beijing, 102600 PR China. Electronic address: liluhai@bigc.edu.cn.
authors: Lu-Hai
authors: LH
authors: Li
authors: Beijing Engineering Research Center of Printed Electronics, Beijing Institute of Graphic Communication, Beijing, 102600 PR China; Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing, 100084 PR China. Electronic address: weiyen@tsinghua.edu.cn.
authors: Yen
authors: Y
authors: Wei
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004867:Equipment Design; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008958:Models, Molecular; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000097:blood*; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D010087:Oxides; D012834:Silver; D006108:Graphite; D017430:Prostate-Specific Antigen
keywords: Enhanced conductivity; Label-free immunosensor; Prostate-specific antigen (PSA); RGO/Ag NPs composites