title: Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholerae and are stabilized by a double signaling domain receptor DosM.
abstract: Nearly all motile bacterial cells use a highly sensitive and adaptable sensory system to detect changes in nutrient concentrations in the environment and guide their movements toward attractants and away from repellents. The best-studied bacterial chemoreceptor arrays are membrane-bound. Many motile bacteria contain one or more additional, sometimes purely cytoplasmic, chemoreceptor systems. Vibrio cholerae contains three chemotaxis clusters (I, II, and III). Here, using electron cryotomography, we explore V. cholerae's cytoplasmic chemoreceptor array and establish that it is formed by proteins from cluster I. We further identify a chemoreceptor with an unusual domain architecture, DosM, which is essential for formation of the cytoplasmic arrays. DosM contains two signaling domains and spans the two-layered cytoplasmic arrays. Finally, we present evidence suggesting that this type of receptor is important for the structural stability of the cytoplasmic array.
authors: Department of Biology, California Institute of Technology, Pasadena, CA 91125 a.briegel@biology.leidenuniv.nl jensen@caltech.edu.
authors: Ariane
authors: A
authors: Briegel
authors: Department of Biology, California Institute of Technology, Pasadena, CA 91125.
authors: Davi R
authors: DR
authors: Ortega
authors: Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, D-35043 Marburg, Germany.
authors: Petra
authors: P
authors: Mann
authors: Department of Biology, California Institute of Technology, Pasadena, CA 91125.
authors: Andreas
authors: A
authors: Kjær
authors: Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, D-35043 Marburg, Germany.
authors: Simon
authors: S
authors: Ringgaard
authors: Department of Biology, California Institute of Technology, Pasadena, CA 91125 a.briegel@biology.leidenuniv.nl jensen@caltech.edu.
authors: Grant J
authors: GJ
authors: Jensen
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002628:Chemoreceptor Cells / Q000737:chemistry / Q000378:metabolism*; D002633:Chemotaxis / Q000235:genetics*; D020285:Cryoelectron Microscopy; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism*; D000072417:Protein Domains; D014054:Tomography; D014734:Vibrio cholerae / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins
keywords: Vibrio cholerae; chemoreceptor arrays; chemotaxis; electron cryotomography; microscopy