title: Characterization of Collagen Type I and II Blended Hydrogels for Articular Cartilage Tissue Engineering.
abstract: Biomaterials that provide signals present in the native extracellular matrix have been proposed as scaffolds to support improved cartilage regeneration. This study harnesses the biological activity of collagen type II and the superior mechanical properties of collagen type I by characterizing gels made of collagen type I and II blends. The collagen blend hydrogels were able to incorporate both types of collagen and retained chondroitin sulfate and hyaluronic acid. Cryo-scanning electron microscopy images showed that the 3:1 ratio of collagen type I to type II gels had a lower void space percentage (36.4%) than the 1:1 gels (46.5%). The complex modulus was larger for the 3:1 gels (G* = 5.0 Pa) compared to the 1:1 gels (G* = 1.2 Pa). The 3:1 blend consistently formed gels with superior mechanical properties compared to the other blends and has the potential to be implemented as a scaffold for articular cartilage engineering.
authors: Weldon School of Biomedical Engineering, Purdue University , West Lafayette, Indiana 47907-2032, United States.
authors: Nelda
authors: N
authors: Vázquez-Portalatı N
authors: School of Chemical Engineering, Purdue University , West Lafayette, Indiana 47907-2100, United States.
authors: Claire E
authors: CE
authors: Kilmer
authors: Weldon School of Biomedical Engineering, Purdue University , West Lafayette, Indiana 47907-2032, United States.
authors: Alyssa
authors: A
authors: Panitch
authors: Weldon School of Biomedical Engineering, Purdue University , West Lafayette, Indiana 47907-2032, United States.|School of Chemical Engineering, Purdue University , West Lafayette, Indiana 47907-2100, United States.
authors: Julie C
authors: JC
authors: Liu
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use; D002358:Cartilage, Articular / Q000187:drug effects* / Q000254:growth & development; D019902:Chondrocytes / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000737:chemistry; D024042:Collagen Type I / Q000008:administration & dosage / Q000737:chemistry; D024043:Collagen Type II / Q000008:administration & dosage / Q000737:chemistry; D005109:Extracellular Matrix / Q000187:drug effects*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D020100:Hydrogels / Q000008:administration & dosage / Q000737:chemistry; D012038:Regeneration / Q000187:drug effects*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D024043:Collagen Type II; D020100:Hydrogels; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates