title: Analytical Methods for Secondary Metabolite Detection.
abstract: The entomopathogenic fungi Metarhizium brunneum, Beauveria bassiana, and B. brongniartii are widely applied as biological pest control agent in OECD countries. Consequently, their use has to be flanked by a risk management approach, which includes the need to monitor the fate of their relevant toxic metabolites. There are still data gaps claimed by regulatory authorities pending on their identification and quantification of relevant toxins or secondary metabolites. In this chapter, analytical methods are presented allowing the qualitative and quantitative analysis of the relevant toxic B. brongniartii metabolite oosporein and the three M. brunneum relevant destruxin (dtx) derivatives dtx A, dtx B, and dtx E.
authors: Institute of Pharmacy, Department of Pharmacognosy, CCB - Centrum of Chemistry and Biomedicine, University of Innsbruck, Innrain 80/82, 6020, Innsbruck, Austria.|Institute of Microbiology, University of Innsbruck, Technikerstraße 25, 6020, Innsbruck, Austria.
authors: Judith
authors: J
authors: Taibon
authors: Institute of Microbiology, University of Innsbruck, Technikerstraße 25, 6020, Innsbruck, Austria. Hermann.Strasser@uibk.ac.at.
authors: Hermann
authors: H
authors: Strasser
mesh_terms: D052982:Beauveria / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D052981:Metarhizium / Q000378:metabolism; D003904:Mitosporic Fungi / Q000378:metabolism*; D015203:Reproducibility of Results; D064210:Secondary Metabolism; D053719:Tandem Mass Spectrometry
keywords: Analyte monitoring; Analytical tool; Destruxin; HPLC–DAD; HPLC–DAD–QTOF–MS/MS; Oosporein; Persistence; Risk assessment; Secondary metabolite; Toxin