title: In Vitro Susceptibility of Sporothrix brasiliensis to Essential Oils of Lamiaceae Family.
abstract: This study evaluated the chemical, cytotoxic and anti-Sporothrix brasiliensis properties of commercial essential oils of rosemary (Rosmarinus officinalis L.), oregano (Origanum vulgare L.) and marjoram (Origanum majorana L.). Chemical composition of the oils was identified through gas chromatography with flame ionization detector, and cytotoxicity was performed through MTT assay in VERO cell line. Anti-S. brasiliensis activity was performed according to the CLSI M38-A2 guidelines using isolates obtained from cats and dogs. The major compounds found were carvacrol in the oregano oil (73.9 %) and 1,8-cineole in rosemary and marjoram oils (49.4 and 20.9 %, respectively). All S. brasiliensis isolates were susceptible to the plant oils, including itraconazole-resistant ones. Marjoram and rosemary oils showed MIC90of 0.56 and 1.12 mg ml-1, and MFC90of 4.5 and 9 mg ml-1, respectively. For oregano oil, a strong antifungal activity was observed with MIC90and MFC90values ≤0.07 mg ml-1. The weakest cytotoxicity was observed for rosemary oil. Further studies should be undertaken to evaluate the safety and efficacy of these essential oils in sporotrichosis.
authors: Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), 1 Campus Universitário Capão do Leão, Pelotas, RS, 96010-900, Brazil. waller.stefanie@yahoo.com.br.
authors: Stefanie Bressan
authors: http://orcid.org/0000-0001-6719-1794
authors: SB
authors: Waller
authors: Centro de Controle de Zoonoses (CCZ), Pelotas, RS, Brazil.
authors: Isabel Martins
authors: IM
authors: Madrid
authors: Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), 1 Campus Universitário Capão do Leão, Pelotas, RS, 96010-900, Brazil.
authors: Anna Luiza
authors: AL
authors: Silva
authors: Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), 1 Campus Universitário Capão do Leão, Pelotas, RS, 96010-900, Brazil.
authors: Luciana Laitano
authors: LL
authors: Dias de Castro
authors: Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), 1 Campus Universitário Capão do Leão, Pelotas, RS, 96010-900, Brazil.
authors: Marlete Brum
authors: MB
authors: Cleff
authors: Laboratório de Cromatografia, Instituto de Química, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors: Vanny
authors: V
authors: Ferraz
authors: Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), 1 Campus Universitário Capão do Leão, Pelotas, RS, 96010-900, Brazil.
authors: Mário Carlos Araújo
authors: MC
authors: Meireles
authors: Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors: Régis
authors: R
authors: Zanette
authors: Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors: João Roberto Braga
authors: JR
authors: de Mello
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002371:Cat Diseases / Q000382:microbiology; D002415:Cats; D002470:Cell Survival / Q000187:drug effects; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D031341:Origanum / Q000737:chemistry*; D064209:Phytochemicals / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D027542:Rosmarinus / Q000737:chemistry*; D013175:Sporothrix / Q000187:drug effects* / Q000302:isolation & purification; D013174:Sporotrichosis / Q000382:microbiology / Q000662:veterinary; D014709:Vero Cells
chemical_list: D000935:Antifungal Agents; D009822:Oils, Volatile; D064209:Phytochemicals
keywords: Chemical composition; Cytotoxicity; Origanum majorana L.; Origanum vulgare L.; Rosmarinus officinalis L.; Sporotrichosis