title: Carcinogen 7,12-dimethylbenz[a]anthracene-induced mammary tumorigenesis is accelerated in Smad3 heterozygous mice compared to Smad3 wild type mice.
abstract: Previous studies based on cell culture and xenograft animal models suggest that Smad3 has tumor suppressor function for breast cancer during early stages of tumorigenesis. In this report, we show that DMBA (7,12-dimethylbenz[a]anthracene), a chemical carcinogen, induces mammary tumor formation at a significantly higher frequency in the Smad3 heterozygous mice than in the Smad3 wild type mice. This is the first genetic evidence showing that Smad3 inhibits mammary tumor formation in a mouse model. Our findings support the notion that Smad3 has important tumor suppressor function for breast cancer.
authors: Center for Advanced Biotechnology and Medicine, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.|Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.|Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, USA.|College of Life Science & Engineering, Chongqing Three Gorges University, Chongqing, China.
authors: Zhengxue
authors: Z
authors: Liu
authors: Center for Advanced Biotechnology and Medicine, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.|Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.|Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, USA.
authors: Tanima
authors: T
authors: Kundu-Roy
authors: Center for Advanced Biotechnology and Medicine, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.|Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.|Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, USA.|Division of Molecular Genomics and Medicine, National Health Research Institutes, Zhunan, Miaoli County, Taiwan.
authors: Isao
authors: I
authors: Matsuura
authors: Center for Advanced Biotechnology and Medicine, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.|Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.|Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, USA.
authors: Guannan
authors: G
authors: Wang
authors: Department of Biostatistics, School of Public Health, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
authors: Yong
authors: Y
authors: Lin
authors: Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
authors: You-Rong
authors: YR
authors: Lou
authors: Department of Pathology, Rutgers Robert Wood Johnson Medical School, Piscataway, NJ, USA.
authors: Nicola J
authors: NJ
authors: Barnard
authors: Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC, USA.
authors: Xiao-Fan
authors: XF
authors: Wang
authors: Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
authors: Mou-Tuan
authors: MT
authors: Huang
authors: Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.|Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, USA.
authors: Nanjoo
authors: N
authors: Suh
authors: Center for Advanced Biotechnology and Medicine, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.|Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.|Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, USA.
authors: Fang
authors: F
authors: Liu
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000230:Adenocarcinoma / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D063646:Carcinogenesis / Q000235:genetics; D002273:Carcinogens / Q000633:toxicity; D005260:Female; D006579:Heterozygote; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D051900:Smad3 Protein / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002273:Carcinogens; D051900:Smad3 Protein; C497791:Smad3 protein, mouse; D025521:Tumor Suppressor Proteins; D015127:9,10-Dimethyl-1,2-benzanthracene
keywords: DMBA; Smad3; TGF-ß; breast cancer; carcinogenesis