title: An ELISA method detecting the active form of suPAR.
abstract: Urokinase plasminogen activator receptor (uPAR) exists in a number of formats in human plasma, including soluble uPAR (suPAR) and uPAR fragments. We developed an ELISA method to detect specifically the active form suPAR, which binds to its natural ligand uPA. The intra CV and inter CV of this ELISA assay is 8.5% and 9.6% respectively, and the assay can recover 99.74% of added recombinant suPAR from 10% plasma. This assay is quite sensitive, capable of detecting down to 15pg/ml of suPAR, and can measure suPAR concentrations in the range of 0.031-8ng/ml with high linear relationship. Plasma samples from pregnant women were also measured for the active form of suPAR with this assay, giving an averaged level of 1.39ng/ml, slightly higher than the level of pooled plasma from healthy donors (0.96ng/ml). This study demonstrates the feasibility to measure the active form of suPAR, which will likely have value in clinical applications.
authors: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.
authors: Xiaolei
authors: X
authors: Zhou
authors: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Mingming
authors: M
authors: Xu
authors: Fujian Maternal and Children Health Hospital Fuzhou, Fujian 350001, China.
authors: Hailong
authors: H
authors: Huang
authors: Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Evanston, IL 60208, USA.
authors: Andrew
authors: A
authors: Mazar
authors: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China; Department of Chemistry, COMSATS Institute of Information Technology (CIIT), Abbottabad, KPK, 22060, Pakistan.
authors: Zafar
authors: Z
authors: Iqbal
authors: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China; College of Bioscience and Biotechnology, Fuzhou University, 2 Xueyuan Road, College Town, Fuzhou, Fujian 350108, China. Electronic address: yuancai@fjirsm.ac.cn.
authors: Cai
authors: C
authors: Yuan
authors: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China; University of Chinese Academy of Sciences, Beijing 100049, China; College of Chemistry, Fuzhou University, 2 Xueyuan Road, College Town, Fuzhou, Fujian 350108, China. Electronic address: T16029@fzu.edu.cn.
authors: Mingdong
authors: M
authors: Huang
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D057230:Limit of Detection; D051379:Mice; D011247:Pregnancy; D055293:Receptors, Urokinase Plasminogen Activator / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D011994:Recombinant Proteins / Q000097:blood / Q000737:chemistry / Q000276:immunology; D000075462:Serum Albumin, Human / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D055293:Receptors, Urokinase Plasminogen Activator; D011994:Recombinant Proteins; D000075462:Serum Albumin, Human
keywords: Active suPAR; ELISA; Pregnant women; suPAR