title: Histone deacetylase HDA6 enhances brassinosteroid signaling by inhibiting the BIN2 kinase.
abstract: Glycogen synthase kinase 3 (GSK3)-like kinases play important roles in brassinosteroid (BR), abscisic acid, and auxin signaling to regulate many aspects of plant development and stress responses. The Arabidopsis thaliana GSK3-like kinase BR-INSENSITIVE 2 (BIN2) acts as a key negative regulator in the BR signaling pathway, but the mechanisms regulating BIN2 function remain unclear. Here we report that the histone deacetylase HDA6 can interact with and deacetylate BIN2 to repress its kinase activity. The hda6 mutant showed a BR-repressed phenotype in the dark and was less sensitive to BR biosynthesis inhibitors. Genetic analysis indicated that HDA6 regulates BR signaling through BIN2. Furthermore, we identified K189 of BIN2 as an acetylated site, which can be deacetylated by HDA6 to influence BIN2 activity. Glucose can affect the acetylation level of BIN2 in plants, indicating a connection to cellular energy status. These findings provide significant insights into the regulation of GSK3-like kinases in plant growth and development.
authors: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai 200433, China;
authors: Yuhan
authors: Y
authors: Hao
authors: College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Haijiao
authors: H
authors: Wang
authors: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai 200433, China;
authors: Shenglong
authors: S
authors: Qiao
authors: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai 200433, China;
authors: Linna
authors: L
authors: Leng
authors: College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China xlwang@mail.hzau.edu.cn.
authors: Xuelu
authors: X
authors: Wang
mesh_terms: D000107:Acetylation; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D060406:Brassinosteroids / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant; D005947:Glucose / Q000378:metabolism; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D050505:Mutant Proteins / Q000235:genetics* / Q000378:metabolism; D063245:Plant Development / Q000235:genetics; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D005947:Glucose; D006655:Histone Deacetylases; D050505:Mutant Proteins; D011494:Protein Kinases; C448985:At4g18710 protein, Arabidopsis; C432452:AT5G63110 protein, Arabidopsis
keywords: BIN2; HDA6; brassinosteroid signaling; deacetylation; development