title: Selenylation modification can enhance immune-enhancing activity of Chuanminshen violaceum polysaccharide.
abstract: Chuanminshen violaceum polysaccharides (CVPS) were extracted, purified and selenizingly modified. The modification has been achieved by using the HNO3- Na2SeO3 method, and selenizing Chuanminshen violaceum polysaccharides (sCVPS) were evaluated for their physicochemical properties and their potential as adjuvant to modulate cellular and humoral immune responses to hepatitis B subunit vaccine in a mouse model. Our results demonstrated that sCVPS significantly promoted splenocytes proliferation and the production of IL-4 and IFN-γ in vitro. In vivo experiments showed that sCVPS significantly increased the rHBsAg-specific IgG level, IgG subclass (IgG1, IgG2a, and IgG2b) antibody titers, T cells proliferation, levels of IL-4, IL-2, and IFN-γ in CD4 (+)T cells and the level of IFN-γ in CD8(+)T cells. Furthermore, sCVPS increased the activity of natural killer cells and cytotoxic T lymphocytes, thus increasing both cellular and humoral immune responses in vivo. The present data suggest that selenylation of CVPS can significantly improve their immune-enhancing activity both in vitro and in vivo, thus representing a powerful adjuvant for vaccine design.
authors: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China. Electronic address: fenghaiborc@126.com.
authors: Feng
authors: F
authors: Haibo
authors: Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan 610051, PR China.
authors: Jing
authors: J
authors: Fan
authors: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors: Hongquan
authors: H
authors: Bo
authors: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors: Xi
authors: X
authors: Tian
authors: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors: He
authors: H
authors: Bao
authors: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors: Xiaohua
authors: X
authors: Wang
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D016207:Cytokines / Q000276:immunology; D005260:Female; D017325:Hepatitis B Vaccines / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D056724:Immunity, Humoral / Q000187:drug effects; D007074:Immunoglobulin G / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D017942:Nitric Acid / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D018038:Sodium Selenite / Q000737:chemistry*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D017325:Hepatitis B Vaccines; D007074:Immunoglobulin G; D011134:Polysaccharides; D022223:Vaccines, Subunit; D017942:Nitric Acid; D018038:Sodium Selenite
keywords: Adjuvant; Chuanminshen violaceum polysaccharide; Selenylation modification