title: Influence of Lipid Core Material on Physicochemical Characteristics of an Ursolic Acid-Loaded Nanostructured Lipid Carrier: An Attempt To Enhance Anticancer Activity.
abstract: The impact of saturation and unsaturation in the fatty acyl hydrocarbon chain on the physicochemical properties of nanostructured lipid carriers (NLCs) was investigated to develop novel delivery systems loaded with an anticancer drug, ursolic acid (UA). Aqueous NLC dispersions were prepared by a high-pressure homogenization-ultrasonication technique with Tween 80 as a stabilizer. Mutual miscibility of the components at the air-water interface was assessed by surface pressure-area measurements, where attractive interactions were recorded between the lipid mixtures and UA, irrespective of the extent of saturation or unsaturation in fatty acyl chains. NLCs were characterized by combined dynamic light scattering, transmission electron microscopy (TEM), atomic force microscopy (AFM), differential scanning calorimetry, drug encapsulation efficiency, drug payload, in vitro drug release, and in vitro cytotoxicity studies. The saturated lipid-based NLCs were larger than unsaturated lipids. TEM and AFM images revealed the spherical and smooth surface morphology of NLCs. The encapsulation efficiency and drug payload were higher for unsaturated lipid blends. In vitro release studies indicate that the nature of the lipid matrix affects both the rate and release pattern. All UA-loaded formulations exhibited superior anticancer activity compared to that of free UA against human leukemic cell line K562 and melanoma cell line B16.
authors: Department of Chemistry, University of North Bengal , Darjeeling 734 013, West Bengal, India.
authors: Prasant
authors: P
authors: Nahak
authors: Department of Chemistry, University of North Bengal , Darjeeling 734 013, West Bengal, India.
authors: Gourab
authors: G
authors: Karmakar
authors: Department of Biotechnology, University of North Bengal , Darjeeling 734 013, West Bengal, India.
authors: Priyam
authors: P
authors: Chettri
authors: Department of Chemistry, University of North Bengal , Darjeeling 734 013, West Bengal, India.
authors: Biplab
authors: B
authors: Roy
authors: Department of Chemistry, University of North Bengal , Darjeeling 734 013, West Bengal, India.
authors: Pritam
authors: P
authors: Guha
authors: Cancer Biology and Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology , 4, Raja S. C. Mullick Road, Kolkata 700032,West Bengal, India.
authors: Shila Elizabeth
authors: SE
authors: Besra
authors: Cancer Biology and Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology , 4, Raja S. C. Mullick Road, Kolkata 700032,West Bengal, India.
authors: Anjana
authors: A
authors: Soren
authors: Department of Colloid Chemistry, St. Petersburg State University , Universitetsky pr. 26, 198504 St. Petersburg, Russia.
authors: Alexey G
authors: AG
authors: Bykov
authors: Department of Colloid Chemistry, St. Petersburg State University , Universitetsky pr. 26, 198504 St. Petersburg, Russia.
authors: Alexander V
authors: AV
authors: Akentiev
authors: Department of Colloid Chemistry, St. Petersburg State University , Universitetsky pr. 26, 198504 St. Petersburg, Russia.
authors: Boris A
authors: BA
authors: Noskov
authors: Department of Chemistry and Chemical Technology, Vidyasagar University , Midnapore 721 102, West Bengal, India.
authors: Amiya Kumar
authors: AK
authors: Panda
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002152:Calorimetry, Differential Scanning; D045744:Cell Line, Tumor; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D049329:Nanostructures; D014315:Triterpenes / Q000737:chemistry*; D000097245:Ursolic Acid
chemical_list: D000970:Antineoplastic Agents; D008055:Lipids; D014315:Triterpenes; D000097245:Ursolic Acid