title: Monitoring the electrochemical responses of neurotransmitters through localized surface plasmon resonance using nanohole array.
abstract: In this study, a novel spectroelectrochemical method was proposed for neurotransmitters detection. The central sensing device was a hybrid structure of nanohole array and gold nanoparticles, which demonstrated good conductivity and high localized surface plasmon resonance (LSPR) sensitivity. By utilizing such specially-designed nanoplasmonic sensor as working electrode, both electrical and spectral responses on the surface of the sensor could be simultaneously detected during the electrochemical process. Cyclic voltammetry was implemented to activate the oxidation and recovery of dopamine and serotonin, while transmission spectrum measurement was carried out to synchronously record to LSPR responses of the nanoplasmonic sensor. Coupling with electrochemistry, LSPR results indicated good integrity and linearity, along with promising accuracy in qualitative and quantitative detection even for mixed solution and in brain tissue homogenates. Also, the detection results of other negatively-charged neurotransmitters like acetylcholine demonstrated the selectivity of our detection method for transmitters with positive charge. When compared with traditional electrochemical signals, LSPR signals provided better signal-to-noise ratio and lower detection limits, along with immunity against interference factors like ascorbic acid. Taking the advantages of such robustness, the coupled detection method was proved to be a promising platform for point-of-care testing for neurotransmitters.
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 PR China; Collaborative Innovation Center of TCM Health Management, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122 PR China.
authors: Nantao
authors: N
authors: Li
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 PR China; Collaborative Innovation Center of TCM Health Management, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122 PR China.
authors: Yanli
authors: Y
authors: Lu
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 PR China.
authors: Shuang
authors: S
authors: Li
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 PR China.
authors: Qian
authors: Q
authors: Zhang
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 PR China.
authors: Jiajia
authors: J
authors: Wu
authors: Micro and Nanotechnology Lab, University of Illinois at Urbana-Champaign, IL, 61801 USA.
authors: Jing
authors: J
authors: Jiang
authors: Micro and Nanotechnology Lab, University of Illinois at Urbana-Champaign, IL, 61801 USA.
authors: Gang Logan
authors: GL
authors: Liu
authors: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 PR China; Collaborative Innovation Center of TCM Health Management, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122 PR China. Electronic address: qjliu@zju.edu.cn.
authors: Qingjun
authors: Q
authors: Liu
mesh_terms: D015374:Biosensing Techniques; D004298:Dopamine / Q000737:chemistry / Q000302:isolation & purification*; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D018377:Neurotransmitter Agents / Q000737:chemistry / Q000302:isolation & purification*; D020349:Surface Plasmon Resonance
chemical_list: D018377:Neurotransmitter Agents; D006046:Gold; D004298:Dopamine
keywords: Coupled detection; Cyclic voltammetry (CV); Localized surface plasmon resonance (LSPR); Neurotransmitters; Spectroelectrochemistry