title: Rationally designed particle preloading method to improve protein delivery performance of electrospun polyester nanofibers.
abstract: Particle preloading method by first loading proteins onto nano- or microparticles and then integrating these particles into electrospun polyester nanofibers has been widely used to encapsulate therapeutic proteins into polyester nanofibers. However, poor method design has resulted in unsatisfactory protein delivery performance. For example, the harsh conditions involved in preloading procedures damage the bioactivities of proteins, the improper integration leads to an uneven distribution of particles in nanofibers or insecure attachment of particles to nanofibers, producing uncontrolled protein release profiles. This study aimed to improve the protein delivery performance of polyester nanofibers by rationally designing a particle preloading method. Positively charged chitosan nanoparticles (CNPs) were used as carriers to adsorb negatively charged proteins in mild conditions and as primary barriers for protein release. The polar CNPs were then homogeneously dispersed in a polar polyester solution and subjected to electrospinning. Microscope observations indicated that CNPs were homogeneously embedded within polyester nanofibers. In vitro release behaviour and cell studies showed that proteins retained their bioactivity and could release from polyester nanofibers in a sustained manner for more than 4 weeks without any initial burst. Epidermal growth factor encapsulated in polyester nanofibers enhanced diabetic wound healing in vivo, demonstrating an application potential in biomedicine. Other properties of the nanofibers, including composition, wettability, cytotoxicity, and cell adhesion and spreading, were examined in detail as well.
authors: Key Laboratory of UV-Emitting Materials and Technology (Northeast Normal University), Ministry of Education, Changchun, Jilin, 130024, China.
authors: Xue
authors: X
authors: Sun
authors: Key Laboratory of UV-Emitting Materials and Technology (Northeast Normal University), Ministry of Education, Changchun, Jilin, 130024, China.
authors: Ke
authors: K
authors: Li
authors: Key Laboratory of UV-Emitting Materials and Technology (Northeast Normal University), Ministry of Education, Changchun, Jilin, 130024, China.
authors: Sainan
authors: S
authors: Chen
authors: Key Laboratory of UV-Emitting Materials and Technology (Northeast Normal University), Ministry of Education, Changchun, Jilin, 130024, China.
authors: Bing
authors: B
authors: Yao
authors: School of Life Sciences, Northeast Normal University, Changchun, Jilin, 130024, China.
authors: Yifa
authors: Y
authors: Zhou
authors: School of Life Sciences, Northeast Normal University, Changchun, Jilin, 130024, China. Electronic address: cuiss100@nenu.edu.cn.
authors: Sisi
authors: S
authors: Cui
authors: Key Laboratory of UV-Emitting Materials and Technology (Northeast Normal University), Ministry of Education, Changchun, Jilin, 130024, China. Electronic address: hujl100@nenu.edu.cn.
authors: Junli
authors: J
authors: Hu
authors: Key Laboratory of UV-Emitting Materials and Technology (Northeast Normal University), Ministry of Education, Changchun, Jilin, 130024, China. Electronic address: ycliu@nenu.edu.cn.
authors: Yichun
authors: Y
authors: Liu
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D065546:Drug Liberation; D004815:Epidermal Growth Factor / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D014945:Wound Healing / Q000187:drug effects
chemical_list: D011091:Polyesters; D004815:Epidermal Growth Factor; D048271:Chitosan
keywords: Particle preloading; Polyester nanofibers; Protein; Rational design