title: Cytogenetic analysis of micronuclei and cell death parameters in epithelial cells of pesticide exposed tea garden workers.
abstract: Buccal micronucleus cytome assay was carried out in 47 exposed (sprayers and leaf harvesters), 47 non-exposed (controls) to determine the extent of damage working in the tea plantations of Terai region of West Bengal, India. As the pesticide exposed male workers were found to consume alcohol and smoked cigarettes/bidis, 35 smokers and 30 alcoholics were also included for comparison. Results showed a significant difference in micronuclei (9.91 ± 2.74, p ≤ .001), nuclear bud (4.98 ± 1.31, p ≤ .001), binucleate (6.26 ± 2.84, p ≤ .001), karyorrhectic (8.36 ± 2.28, p ≤ .001), pyknotic (5.62 ± 1.78, p ≤ .05) as well as karyolytic (6.81 ± 3.00, p ≤ .001) nuclei compared with control. Comparison also revealed a higher frequency of micronuclei (6.11 ± 2.55, p ≤ .01), nuclear bud (4.06 ± 1.97, p ≤ .05), binucleate (4.34 ± 1.85, p ≤ .001), karyorrhectic (6.83 ± 2.12, p ≤ .001), and karyolytic (6.20 ± 2.54, p ≤ .001) nuclei except pyknotic cell in the smoker than control. Frequency of binucleate (3.80 ± 1.73, p ≤ .05), karyorrhectic (5.57 ± 2.34, p ≤ .05), pyknotic (5.50 ± 1.36, p ≤ .05), and karyolytic (6.30 ± 2.71, p ≤ .001) nuclei was higher in the alcoholics than control (non-alcoholics), whereas the micronuclei and nuclear bud were found to be non-significant compared with the control. Our analyses also revealed a higher proportion of the micronucleus and the cell death parameters in the pesticide exposed males than females, which indicated that pesticide, smoking, and alcohol may act synergistically to cause more damage to the buccal epithelial cells. However, age and the exposure duration have no influence on the micronucleus and other cell death parameters.
authors: a Genetics and Molecular Biology Laboratory, Department of Zoology , University of North Bengal , Dist. Darjeeling , West Bengal , India.
authors: Susmita
authors: S
authors: Dutta
authors: a Genetics and Molecular Biology Laboratory, Department of Zoology , University of North Bengal , Dist. Darjeeling , West Bengal , India.
authors: Min
authors: M
authors: Bahadur
mesh_terms: D000328:Adult; D000428:Alcohol Drinking / Q000009:adverse effects; D028241:Camellia sinensis / Q000254:growth & development*; D016022:Case-Control Studies; D016923:Cell Death / Q000187:drug effects*; D004784:Environmental Monitoring / Q000379:methods; D004847:Epithelial Cells / Q000187:drug effects* / Q000473:pathology; D005260:Female; D006801:Humans; D007194:India; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology; D016273:Occupational Exposure / Q000009:adverse effects* / Q000032:analysis; D010575:Pesticides / Q000633:toxicity*; D012737:Sex Factors; D012907:Smoking / Q000009:adverse effects; D011795:Surveys and Questionnaires; D055815:Young Adult
chemical_list: D010575:Pesticides
keywords: Micronucleus; biomarker; genome damage; pesticide; tea gardens