title: Whole-Genome Sequencing and iPLEX MassARRAY Genotyping Map an EMS-Induced Mutation Affecting Cell Competition in Drosophila melanogaster.
abstract: Cell competition, the conditional loss of viable genotypes only when surrounded by other cells, is a phenomenon observed in certain genetic mosaic conditions. We conducted a chemical mutagenesis and screen to recover new mutations that affect cell competition between wild-type and RpS3 heterozygous cells. Mutations were identified by whole-genome sequencing, making use of software tools that greatly facilitate the distinction between newly induced mutations and other sources of apparent sequence polymorphism, thereby reducing false-positive and false-negative identification rates. In addition, we utilized iPLEX MassARRAY for genotyping recombinant chromosomes. These approaches permitted the mapping of a new mutation affecting cell competition when only a single allele existed, with a phenotype assessed only in genetic mosaics, without the benefit of complementation with existing mutations, deletions, or duplications. These techniques expand the utility of chemical mutagenesis and whole-genome sequencing for mutant identification. We discuss mutations in the Atm and Xrp1 genes identified in this screen.
authors: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461.
authors: Chang-Hyun
authors: CH
authors: Lee
authors: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461.
authors: Gerard
authors: G
authors: Rimesso
authors: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461.
authors: David M
authors: DM
authors: Reynolds
authors: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461.
authors: Jinlu
authors: J
authors: Cai
authors: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461 nicholas.baker@einstein.yu.edu.
authors: Nicholas E
authors: NE
authors: Baker
mesh_terms: D000483:Alleles; D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins / Q000235:genetics; D002874:Chromosome Mapping; D004260:DNA Repair; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D056726:Genetic Association Studies; D005820:Genetic Testing; D049750:Genome, Insect; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D016296:Mutagenesis; D009154:Mutation / Q000187:drug effects*; D010641:Phenotype; D019655:Quantitative Trait, Heritable; D011995:Recombination, Genetic
chemical_list: D005020:Ethyl Methanesulfonate; D064007:Ataxia Telangiectasia Mutated Proteins
keywords: Drosophila melanogaster; Flybook; Xrp1; cell competition; iPLEX MassARRAY; whole-genome sequencing