title: Lab-on-a-Chip-Surface Enhanced Raman Scattering Combined with the Standard Addition Method: Toward the Quantification of Nitroxoline in Spiked Human Urine Samples.
abstract: The emergence of antibacterial resistance and the development of new drugs lead to a continuous change of guidelines for medical treatments. Hence, new analytical tools are required for the detection of drugs in biological fluids. In this study, the first surface enhanced Raman scattering (SERS) detection of nitroxoline (NTX) in purified water and in spiked human urine samples is reported. Insights concerning the nature of the molecule-metal interaction and its influence on the overall SERS signal are provided. Furthermore, three randomly collected urine samples originating from a healthy volunteer were spiked to assess the limit of detection (LOD), the limit of quantification (LOQ), and the linear dynamic range of the lab-on-a-chip SERS (LoC-SERS) method for NTX detection in human urine. The LOD is ∼3 μM (0.57 mg/L), LOQ ∼ 6.5 μM (1.23 mg/L) while the linear range is between 4.28 and 42.8 μM (0.81-8.13 mg/L). This covers the minimum inhibitory concentration (MIC) values of the most commonly encountered uropathogens. Finally, seven clinical samples having an "unknown" NTX concentration were simulated. The LoC-SERS technique combined with the standard addition method and statistical data analysis provided a good prediction of the unknown concentrations. Additionally, it is also demonstrated that the predictions carried out by multicurve resolution alternating least-squares (MCR-ALS) algorithm provides reliable results, and it is preferred to a univariate statistical approach.
authors: Friedrich Schiller University Jena , Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07745 Jena, Germany.|Leibniz Institute of Photonic Technology Jena , Albert-Einstein-Strasse 9, 07745 Jena, Germany.
authors: Izabella J
authors: IJ
authors: Hidi
authors: Friedrich Schiller University Jena , Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07745 Jena, Germany.|Leibniz Institute of Photonic Technology Jena , Albert-Einstein-Strasse 9, 07745 Jena, Germany.
authors: Martin
authors: M
authors: Jahn
authors: Friedrich Schiller University Jena , Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07745 Jena, Germany.|Leibniz Institute of Photonic Technology Jena , Albert-Einstein-Strasse 9, 07745 Jena, Germany.|Research Campus Infectognostic , Philosophenweg 7, 07743 Jena, Germany.
authors: Karina
authors: K
authors: Weber
authors: Friedrich Schiller University Jena , Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07745 Jena, Germany.
authors: Thomas
authors: T
authors: Bocklitz
authors: Center for Infectious Diseases and Infection Control, Jena University Hospital , Erlanger Allee 101 07740 Jena, Germany.|Research Campus Infectognostic , Philosophenweg 7, 07743 Jena, Germany.
authors: Mathias W
authors: MW
authors: Pletz
authors: Friedrich Schiller University Jena , Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07745 Jena, Germany.|Leibniz Institute of Photonic Technology Jena , Albert-Einstein-Strasse 9, 07745 Jena, Germany.|Research Campus Infectognostic , Philosophenweg 7, 07743 Jena, Germany.
authors: Dana
authors: D
authors: Cialla-May
authors: Friedrich Schiller University Jena , Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07745 Jena, Germany.|Leibniz Institute of Photonic Technology Jena , Albert-Einstein-Strasse 9, 07745 Jena, Germany.|Research Campus Infectognostic , Philosophenweg 7, 07743 Jena, Germany.
authors: Juergen
authors: J
authors: Popp
mesh_terms: D000892:Anti-Infective Agents, Urinary / Q000032:analysis / Q000652:urine*; D004867:Equipment Design; D006801:Humans; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D009600:Nitroquinolines / Q000032:analysis / Q000652:urine*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D014867:Water / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000652:urine
chemical_list: D000892:Anti-Infective Agents, Urinary; D009600:Nitroquinolines; D014874:Water Pollutants, Chemical; D014867:Water; C005308:nitroxoline