title: Effect of Soluble Epoxide Hydrolase on the Modulation of Coronary Reactive Hyperemia: Role of Oxylipins and PPARγ.
abstract: Coronary reactive hyperemia (CRH) is a physiological response to ischemic insult that prevents the potential harm associated with an interruption of blood supply. The relationship between the pharmacologic inhibition of soluble epoxide hydrolase (sEH) and CRH response to a brief ischemia is not known. sEH is involved in the main catabolic pathway of epoxyeicosatrienoic acids (EETs), which are converted into dihydroxyeicosatrienoic acids (DHETs). EETs protect against ischemia/reperfusion injury and have numerous beneficial physiological effects. We hypothesized that inhibition of sEH by t-AUCB enhances CRH in isolated mouse hearts through changing the oxylipin profiles, including an increase in EETs/DHETs ratio. Compared to controls, t-AUCB-treated mice had increased CRH, including repayment volume (RV), repayment duration, and repayment/debt ratio (p < 0.05). Treatment with t-AUCB significantly changed oxylipin profiles, including an increase in EET/DHET ratio, increase in EpOME/DiHOME ratio, increase in the levels of HODEs, decrease in the levels of mid-chain HETEs, and decrease in prostanoids (p < 0.05). Treatment with MS-PPOH (CYP epoxygenase inhibitor) reduced CRH, including RV (p < 0.05). Involvement of PPARγ in the modulation of CRH was demonstrated using a PPARγ-antagonist (T0070907) and a PPARγ-agonist (rosiglitazone). T0070907 reduced CRH (p < 0.05), whereas rosiglitazone enhanced CRH (p < 0.05) in isolated mouse hearts compared to the non-treated. These data demonstrate that sEH inhibition enhances, whereas CYP epoxygenases-inhibition attenuates CRH, PPARγ mediate CRH downstream of the CYP epoxygenases-EET pathway, and the changes in oxylipin profiles associated with sEH-inhibition collectively contributed to the enhanced CRH.
authors: Basic Pharmaceutical Sciences, School of Pharmacy, Center for Basic and Translational Stroke Research, West Virginia University, Morgantown, WV, United States of America.
authors: Ahmad
authors: A
authors: Hanif
authors: Division of Intramural Research, NIEHS/NIH, Research Triangle Park, NC, United States of America.
authors: Matthew L
authors: ML
authors: Edin
authors: Division of Intramural Research, NIEHS/NIH, Research Triangle Park, NC, United States of America.
authors: Darryl C
authors: DC
authors: Zeldin
authors: University of California at Davis, One Shields Avenue, Davis, CA, United States of America.
authors: Christophe
authors: C
authors: Morisseau
authors: Basic Pharmaceutical Sciences, School of Pharmacy, Center for Basic and Translational Stroke Research, West Virginia University, Morgantown, WV, United States of America.
authors: Mohammed A
authors: MA
authors: Nayeem
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D003327:Coronary Disease / Q000201:enzymology / Q000378:metabolism / Q000503:physiopathology*; D004851:Epoxide Hydrolases / Q000378:metabolism*; D006940:Hyperemia / Q000201:enzymology / Q000378:metabolism / Q000503:physiopathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D054883:Oxylipins / Q000378:metabolism*; D047495:PPAR gamma / Q000819:agonists / Q000502:physiology*; D000077154:Rosiglitazone; D053719:Tandem Mass Spectrometry; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: D054883:Oxylipins; D047495:PPAR gamma; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D004851:Epoxide Hydrolases