title: Enzymatic saccharification of acid pretreated corn stover: Empirical and fractal kinetic modelling.
abstract: Enzymatic hydrolysis of corn stover was studied at agitation speeds from 50 to 500rpm in a stirred tank bioreactor, at high solid concentrations (20% w/w dry solid/suspension), 50°C and 15.5mgprotein·gglucane(-1). Two empirical kinetic models have been fitted to empirical data, namely: a potential model and a fractal one. For the former case, the global order dramatically decreases from 13 to 2 as agitation speed increases, suggesting an increment in the access of enzymes to cellulose in terms of chemisorption followed by hydrolysis. For its part, the fractal kinetic model fits better to data, showing its kinetic constant a constant augmentation with increasing agitation speed up to a constant value at 250rpm and above, when mass transfer limitations are overcome. In contrast, the fractal exponent decreases with rising agitation speed till circa 0.19, suggesting higher accessibility of enzymes to the substrate.
authors: Chemical Engineering Department, Universidad Complutense de Madrid, Avda. Complutense s/n, 28040 Madrid, Spain.
authors: Mateusz
authors: M
authors: Wojtusik
authors: Abengoa Research, Campus de Palmas Altas, 41014 Sevilla, Spain.
authors: Mauricio
authors: M
authors: Zurita
authors: Laboratory of Cellulose and Paper, INIA, Forest Research Center, Ctra. de la Coruña km 7.5, 28040 Madrid, Spain.
authors: Juan C
authors: JC
authors: Villar
authors: Chemical Engineering Department, Universidad Complutense de Madrid, Avda. Complutense s/n, 28040 Madrid, Spain. Electronic address: mladerog@ucm.es.
authors: Miguel
authors: M
authors: Ladero
authors: Chemical Engineering Department, Universidad Complutense de Madrid, Avda. Complutense s/n, 28040 Madrid, Spain.
authors: Felix
authors: F
authors: Garcia-Ochoa
mesh_terms: D000143:Acids / Q000378:metabolism*; D019149:Bioreactors; D002480:Cellulase; D002482:Cellulose / Q000378:metabolism*; D017709:Fractals; D006868:Hydrolysis; D007700:Kinetics; D008962:Models, Theoretical; D003313:Zea mays / Q000201:enzymology* / Q000378:metabolism*
chemical_list: D000143:Acids; D002482:Cellulose; D002480:Cellulase
keywords: Corn stover; Empirical model; Enzymatic hydrolysis; Fractal model; Kinetic modelling; Stirrer speed