title: Aptamer-Mediated Transparent-Biocompatible Nanostructured Surfaces for Hepotocellular Circulating Tumor Cells Enrichment.
abstract: Circulating tumor cells (CTCs) have been considered as the origin of cancer metastasis. Thus, detection of CTCs in peripheral blood is of great value in different types of solid tumors. However, owing to extremely low abundance of CTCs, detection of them has been technically challenging. To establish a simple and efficient method for CTCs detection in patients with hepatocellular carcinoma (HCC), we applied biocompatible and transparent HA/CTS (Hydroxyapatite/chitosan) nanofilm to achieve enhanced topographic interactions with nanoscale cellular surface components, and we used sLex-AP (aptamer for carbohydrate sialyl Lewis X) to coat onto HA/CTS nanofilm for efficient capture of HCC CTCs, these two functional components combined to form our CTC-(BioT)Chip platform. Using this platform, we realized HCC CTCs' capture and identification, the average recovery rate was 61.6% or more at each spiking level. Importantly, our platform identified CTCs (2±2 per 2 mL) in 25 of 42 (59.5%) HCC patients. Moreover, both the positivity rate and the number of detected CTCs were significantly correlated with tumor size, portal vein tumor thrombus, and the TNM (tumor-node-metastasis) stage. In summary, our CTC-(BioT)Chip platform provides a new method allowing for simple but efficient detection of CTCs in HCC patients, and it holds potential of clinically usefulness in monitoring HCC prognosis and guiding individualized treatment in the future.
authors: Department of Oncology, Zhongnan Hospital of Wuhan University; Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuhan 430071, P. R. China;
authors: Shuyi
authors: S
authors: Wang
authors: Department of Oncology, Zhongnan Hospital of Wuhan University; Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuhan 430071, P. R. China;
authors: Chunxiao
authors: C
authors: Zhang
authors: Department of Oncology, Zhongnan Hospital of Wuhan University; Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuhan 430071, P. R. China;
authors: Guozhou
authors: G
authors: Wang
authors: Department of Oncology, Zhongnan Hospital of Wuhan University; Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuhan 430071, P. R. China;; Department of Oncology, Peking University Shenzhen Hospital, No. 1120, Lianhua Road, Futain District, Shenzhen 518036, Guangdong Province, P. R. China.
authors: Boran
authors: B
authors: Cheng
authors: Department of Oncology, Zhongnan Hospital of Wuhan University; Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuhan 430071, P. R. China;; Department of Breast Cancer, Cancer Center, Guangdong General Hospital, Guangzhou 510050, Guangdong Province, P.R. China.
authors: Yulei
authors: Y
authors: Wang
authors: Department of Oncology, Zhongnan Hospital of Wuhan University; Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuhan 430071, P. R. China;
authors: Fangfang
authors: F
authors: Chen
authors: Department of Oncology, Zhongnan Hospital of Wuhan University; Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuhan 430071, P. R. China;
authors: Yuanyuan
authors: Y
authors: Chen
authors: Department of Oncology, Zhongnan Hospital of Wuhan University; Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuhan 430071, P. R. China;
authors: Maohui
authors: M
authors: Feng
authors: Department of Oncology, Zhongnan Hospital of Wuhan University; Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuhan 430071, P. R. China;
authors: Bin
authors: B
authors: Xiong
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000556:secondary; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D048271:Chitosan; D017886:Durapatite; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000556:secondary; D049329:Nanostructures / Q000737:chemistry*; D009360:Neoplastic Cells, Circulating; D013499:Surface Properties
chemical_list: D052157:Aptamers, Nucleotide; D048271:Chitosan; D017886:Durapatite
keywords: Aptamer; Cell capture; Circulating tumor cells; Hepatocellular carcinoma; Hydroxyapatite/chitosan.; Nanomaterial