title: Proteomics of Physcomitrella patens protonemata subjected to treatment with 12-oxo-phytodienoic acid.
abstract: 12-Oxo-phytodienoic acid (OPDA) is induced by mechanical wounding and suppresses the growth of Physcomitrella patens; OPDA is considered as a signal compound in this moss species. In this study, a proteomic analysis of P. patens protonemata treated with OPDA was performed. The abundance levels of 41 proteins were significantly altered by OPDA, with decreased levels for 40 proteins. The proteins for which abundance decreased in response to OPDA at the protonema developmental stage were mainly involved in the metabolism of proteins and carbohydrates. The effects of inhibition on protein abundance are likely a major physiological function of OPDA in P. patens. OPDA also suppressed the expression of histones at the protein level and gene transcription level. Suppression of histone expression might be an OPDA-specific function in P. patens protonemata. In P. patens, a subset of the physiological responses caused by OPDA is shown to differ between protonema and gametophore developmental stages.
authors: a Division of Fundamental Agriscience Research, Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors: Weifeng
authors: W
authors: Luo
authors: b National Institute for Crop Science , National Agriculture and Food Research Organization , Tsukuba , Japan.
authors: Yohei
authors: Y
authors: Nanjo
authors: b National Institute for Crop Science , National Agriculture and Food Research Organization , Tsukuba , Japan.
authors: Setsuko
authors: S
authors: Komatsu
authors: a Division of Fundamental Agriscience Research, Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors: Hideyuki
authors: H
authors: Matsuura
authors: a Division of Fundamental Agriscience Research, Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
authors: Kosaku
authors: K
authors: Takahashi
mesh_terms: D019068:Bryopsida / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006657:Histones / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D040901:Proteomics
chemical_list: D005231:Fatty Acids, Unsaturated; D006657:Histones; D010940:Plant Proteins; C025999:12-oxophytodienoic acid
keywords: 12-oxo-phytodienoic acid; Physcomitrella patens; histones; proteomic analysis; protonema