title: Relative Alterations in Blood-Based Levels of Sestrin in Alzheimer's Disease and Mild Cognitive Impairment Patients.
abstract: Sestrins (sesn) are highly conserved proteins that play an important neuroprotective role, in part as a consequence of their antioxidative capacity, which prevents reactive oxygen species formation. In this study, we evaluated the concentrations of sesn1 and sesn2 in the serum of 41 Alzheimer's disease (AD) patients, 27 mild cognitive impairment (MCI), and 60 elderly controls, by surface plasmon resonance, which was validated by using western blot. Moreover, the mRNA level of sestrins in all the study groups was determined by real time polymerase chain reaction. The results showed significant overexpression of serum sesn2 protein and mRNA levels in the AD group compared to MCI and elderly control groups. A difference in serum sesn2 concentration between MCI and the control group was also evident. ROC analysis showed highly sensitive, selective cutoff values for sens2 in the differentiation of AD, MCI, and controls. No significant difference in sesn1 level was observed among the study groups. This study highlights the important role of sesn2 in the progression of the AD, indicating its potential utility as a protein marker in this devastating disease.
authors: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors: Nitish
authors: N
authors: Rai
authors: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors: Rahul
authors: R
authors: Kumar
authors: Department of Geriatric Medicine, All India Institute of Medical Sciences, New Delhi, India.
authors: Gaurav Rajesh
authors: GR
authors: Desai
authors: Department of Geriatric Medicine, All India Institute of Medical Sciences, New Delhi, India.
authors: G
authors: G
authors: Venugopalan
authors: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors: Shashank
authors: S
authors: Shekhar
authors: Department of Geriatric Medicine, All India Institute of Medical Sciences, New Delhi, India.
authors: Prasun
authors: P
authors: Chatterjee
authors: Department of Neurology, All India Institute of Medical Sciences, New Delhi, India.
authors: Manjari
authors: M
authors: Tripathi
authors: Department of Biostatistics, All India Institute of Medical Sciences, New Delhi, India.
authors: Ashish Datt
authors: AD
authors: Upadhyay
authors: Department of Biostatistics, All India Institute of Medical Sciences, New Delhi, India.
authors: Sadanand
authors: S
authors: Dwivedi
authors: Department of Geriatric Medicine, All India Institute of Medical Sciences, New Delhi, India.
authors: Aparajit B
authors: AB
authors: Dey
authors: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors: Sharmistha
authors: S
authors: Dey
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000097:blood* / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood; D060825:Cognitive Dysfunction / Q000097:blood* / Q000175:diagnosis*; D005260:Female; D006360:Heat-Shock Proteins / Q000097:blood*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000097:blood*; D012333:RNA, Messenger / Q000097:blood; D020349:Surface Plasmon Resonance / Q000379:methods; D000084622:Sestrins
chemical_list: D015415:Biomarkers; D006360:Heat-Shock Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C117338:SESN1 protein, human; D000084622:Sestrins; C465398:SESN2 protein, human
keywords: Alzheimer’s disease; marker; mild cognitive impairment; serum; sestrins