title: An in vitro vascular chip using 3D printing-enabled hydrogel casting.
abstract: An important unsolved challenge in tissue engineering has been the inability to replicate the geometry and function of vascular networks and blood vessels. Here, we engineer a user-defined 3D microfluidic vascular channel using 3D printing-enabled hydrogel casting. First, a hollow L-shaped channel is developed using a template casting process. In this process, murine 10T1/2 cells are encapsulated within gelatin methacrylate (GelMA) hydrogel using UV photocrosslinking, and upon removal of the template results in a hollow channel within GelMA. Second, human umbilical vein endothelial cells (HUVECs) were cultured within the channel and immunostaining was used to visualize endothelial monolayers. Third, diffusion/permeability studies on endothelialized channels were carried out to demonstrate the barrier function of HUVEC monolayer. Taken together, we develop a facile, cytocompatible and rapid approach to engineer a user-defined multicellular vascular chip that could be potentially useful in developing new vascular model systems.
authors: Department of Biomedical and Chemical Engineering, Syracuse University, 900 S Crouse Ave, Syracuse NY 13210, USA.
authors: Liang
authors: L
authors: Yang
authors: Shivkumar Vishnempet
authors: SV
authors: Shridhar
authors: Melissa
authors: M
authors: Gerwitz
authors: Pranav
authors: P
authors: Soman
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D003432:Cross-Linking Reagents / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D056656:Lab-On-A-Chip Devices; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D010539:Permeability; D066330:Printing, Three-Dimensional; D033081:Shear Strength; D013552:Swine; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D020100:Hydrogels; D008689:Methacrylates; D005780:Gelatin