title: Expression, Regulation, and Functional Characterization of FST Gene in Porcine Granulosa Cells.
abstract: Proliferation, differentiation, and estrogen secretion of granulosa cells are the key factors affecting the estrous after weaning in sows. The objective of this study was to evaluate the expression of Follistatin (FST) in the ovary of Xiushui Hang and Duroc sows at weaning and estrus, the effect of FSH on transcript abundance of FST gene in granulosa cells and the role of FST gene in the weaning to estrus using siRNAs targeted to FST gene. In the present study, expression of the FST mRNA was evaluated by real time PCR. The FST mRNA levels showed a reduction from weaning to the estrus in both Xiushui Hang and Duroc sows, and the mRNA levels in Duroc ovary was higher than in Xiushui Hang sows at the beginning of estrus. Granulosa cells were obtained from the two largest follicles around follicular deviation, FST expression was decreased sharply after treatment with FSH (250 ng/ml). Knockdown of FST by siRNA in porcine granulosa cells significantly increased cell proliferation and estrogen secretion. These results indicate that FST gene is a negative regulator of follicle growth and function during the weaning-estrus interval.
authors: a Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science , Nanchang , P. R. China.
authors: QuanYong
authors: Q
authors: Zhou
authors: a Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science , Nanchang , P. R. China.
authors: MingChun
authors: M
authors: Wan
authors: a Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science , Nanchang , P. R. China.
authors: QiPeng
authors: Q
authors: Wei
authors: a Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science , Nanchang , P. R. China.
authors: QiongLi
authors: Q
authors: Song
authors: a Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science , Nanchang , P. R. China.
authors: LiGen
authors: L
authors: Xiong
authors: a Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science , Nanchang , P. R. China.
authors: JunHong
authors: J
authors: Huo
authors: a Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science , Nanchang , P. R. China.
authors: JiangNan
authors: J
authors: Huang
mesh_terms: D000818:Animals; D004971:Estrus / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D038681:Follistatin / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D006107:Granulosa Cells / Q000378:metabolism*; D013552:Swine
chemical_list: D038681:Follistatin; D005640:Follicle Stimulating Hormone
keywords: FSH; FST; RNAi; granulosa cell; weaning-estrus interval