title: Clinical Islet Isolation.
abstract: The overarching success of islet transplantation relies on the success in the laboratory to isolate the islets. This chapter focuses on the processes of human islet cell isolation and the ways to optimally provide islet cells for transplantation. The major improvements in regards to the choice of enzyme type, way the digested pancreas tissue is handled to best separate islets from the acinar and surrounding tissues, the various methods of purification of the islets, their subsequent culture and quality assurance to improve outcomes to culminate in safe and effective islet transplantation will be discussed. After decades of improvements, islet cell isolation and transplantation now clearly offer a safe, effective and feasible therapeutic treatment option for an increasing number of patients suffering from type 1 diabetes specifically for those with severe hypoglycaemic unawareness.
authors: National Pancreas and Islet Transplant Laboratories, The Westmead Institute for Medical Research, Westmead, NSW, 2145, Australia. wayne.hawthorne@sydney.edu.au.|Department of Surgery, Westmead Clinical School, Westmead Hospital, University of Sydney, Westmead, NSW, 2145, Australia. wayne.hawthorne@sydney.edu.au.
authors: Wayne J
authors: WJ
authors: Hawthorne
authors: National Pancreas and Islet Transplant Laboratories, The Westmead Institute for Medical Research, Westmead, NSW, 2145, Australia.
authors: Lindy
authors: L
authors: Williams
authors: National Pancreas and Islet Transplant Laboratories, The Westmead Institute for Medical Research, Westmead, NSW, 2145, Australia.
authors: Yi Vee
authors: YV
authors: Chew
mesh_terms: D000818:Animals; D001681:Biological Assay; D002470:Cell Survival; D006801:Humans; D007515:Islets of Langerhans / Q000502:physiology*; D016381:Islets of Langerhans Transplantation; D051379:Mice; D013696:Temperature; D046509:Tissue Culture Techniques / Q000379:methods*
keywords: Diabetes; Insulin; Islet; Islet cell; Islet cell allotransplantation; Islet cell autotransplantation; Islet cell isolation; Islet equivalent (IEQ); Type 1 diabetes (T1D)