title: The conundrum of UDP-Glc entrance into the yeast ER lumen.
abstract: UDP-Glc entrance into the endoplasmic reticulum (ER) of eukaryotic cells is a key step in the quality control of glycoprotein folding, a mechanism requiring transfer of a Glc residue from the nucleotide sugar (NS) to glycoprotein folding intermediates by the UDP-Glc:glycoprotein glucosyltransferase (UGGT). According to a bioinformatics search there are only eight genes in the Schizosaccharomyces pombe genome belonging to the three Pfam families to which all known nucleotide-sugar transporters (NSTs) of the secretory pathway belong. The protein products of two of them (hut1+and yea4+) localize to the ER, those of genes gms1+, vrg4+, pet1+, pet2+and pet3+to the Golgi, whereas that of gms2+has an unknown location. Here we demonstrate that (1) Δhut1 and Δgpt1 (UGGT null) mutants share several phenotypic features; (2) Δhut1 mutants show a 50% reduction in UDP-Glc transport into ER-derived membranes; (3) in vivo UDP-Glc ER entrance occurred in Δhut1Δyea4Δgms2 mutants and in cells in which Δhut1 disruption was combined with that of each of four of the genes encoding Golgi-located proteins. Therefore, disruption of all genes whose products localize to the ER or have an unknown location did not obliterate UDP-Glc ER entrance. We conclude that the hut1+gene product is involved in UDP-Glc entrance into the ER, but that at least another as yet unknown NST displaying an unconventional sequence operates in the yeast secretory pathway. This conclusion agrees with our previous results showing that UDP-Glc entrance into the yeast ER does not follow the classical NST antiport mechanism.
authors: Department of Biological Chemistry and IQUIFIB (CONICET), School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires C1113AAD, Argentine.
authors: Luis M
authors: LM
authors: Bredeston
authors: Fundación Instituto Leloir and IIBBA, CONICET. Av. Patricias Argentinas 435, Buenos Aires C1405BWE, Argentine.
authors: Cristina
authors: C
authors: Marino-Buslje
authors: Fundación Instituto Leloir. Av. Patricias Argentinas 435, Buenos Aires C1405BWE, Argentine.
authors: Vanesa S
authors: VS
authors: Mattera
authors: Fundación Instituto Leloir. Av. Patricias Argentinas 435, Buenos Aires C1405BWE, Argentine.
authors: Lucila I
authors: LI
authors: Buzzi
authors: Fundación Instituto Leloir and IIBBA, CONICET. Av. Patricias Argentinas 435, Buenos Aires C1405BWE, Argentine cdalessio@leloir.org.ar aparodi@leloir.org.ar.
authors: Armando J
authors: AJ
authors: Parodi
authors: Fundación Instituto Leloir and IIBBA, CONICET. Av. Patricias Argentinas 435, Buenos Aires C1405BWE, Argentine cdalessio@leloir.org.ar aparodi@leloir.org.ar.|School of Sciences, University of Buenos Aires, Av Intendente Guiraldes 2160, Buenos Aires C1428EHA, Argentine.
authors: Cecilia
authors: C
authors: D'Alessio
mesh_terms: D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000201:enzymology*; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics*; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006056:Golgi Apparatus / Q000201:enzymology; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics*; D017510:Protein Folding; D012568:Schizosaccharomyces / Q000201:enzymology
chemical_list: D006023:Glycoproteins; D050505:Mutant Proteins; D005964:Glucosyltransferases; C072423:mannosylglycoprotein 1,3-glucosyltransferase
keywords: N-linked glycosylation; Schizosaccharomyces pombe; UDP-Glc; endoplasmic reticulum; nucleotide-sugar transporter