title: EGF and curcumin co-encapsulated nanoparticle/hydrogel system as potent skin regeneration agent.
abstract: Wound healing is a complex multifactorial process that relies on coordinated signaling molecules to succeed. Epidermal growth factor (EGF) is a mitogenic polypeptide that stimulates wound repair; however, precise control over its application is necessary to reduce the side effects and achieve desired therapeutic benefits. Moreover, the extensive oxidative stress during the wound healing process generally inhibits repair of the injured tissues. Topical applications of antioxidants like curcumin (Cur) could protect tissues from oxidative damage and significantly improve tissue remodeling. To achieve much accelerated wound healing effects, we designed a novel dual drug co-loaded in situ gel-forming nanoparticle/hydrogel system (EGF-Cur-NP/H) which acted not only as a supportive matrix for the regenerative tissue, but also as a sustained drug depot for EGF and Cur. In the established excisional full-thickness wound model, EGF-Cur-NP/H treatment significantly enhanced wound closure through increasing granulation tissue formation, collagen deposition, and angiogenesis, relative to normal saline, nanoparticle/hydrogel (NP/H), Cur-NP/H, and EGF-NP/H treated groups. In conclusion, this study provides a biocompatible in situ gel-forming system for efficient topical application of EGF and Cur in the landscape of tissue repair.
authors: State Key Laboratory of Biotherapy and Cancer Center, Department of Neurosurgery, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, People's Republic of China.
authors: Xiaoling
authors: X
authors: Li
authors: College of Life Science, Northeast Agriculture University, Harbin, People's Republic of China.
authors: Xianlong
authors: X
authors: Ye
authors: College of Life Science, Northeast Agriculture University, Harbin, People's Republic of China.
authors: Jianying
authors: J
authors: Qi
authors: State Key Laboratory of Biotherapy and Cancer Center, Department of Neurosurgery, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, People's Republic of China.
authors: Rangrang
authors: R
authors: Fan
authors: State Key Laboratory of Biotherapy and Cancer Center, Department of Neurosurgery, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, People's Republic of China.
authors: Xiang
authors: X
authors: Gao
authors: College of Life Science, Northeast Agriculture University, Harbin, People's Republic of China.
authors: Yunzhou
authors: Y
authors: Wu
authors: State Key Laboratory of Biotherapy and Cancer Center, Department of Neurosurgery, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, People's Republic of China.
authors: Liangxue
authors: L
authors: Zhou
authors: State Key Laboratory of Biotherapy and Cancer Center, Department of Neurosurgery, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, People's Republic of China.
authors: Aiping
authors: A
authors: Tong
authors: State Key Laboratory of Biotherapy and Cancer Center, Department of Neurosurgery, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, People's Republic of China.
authors: Gang
authors: G
authors: Guo
mesh_terms: D016475:3T3 Cells; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D065546:Drug Liberation; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D057809:HEK293 Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D012038:Regeneration / Q000187:drug effects*; D012867:Skin / Q000187:drug effects*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011091:Polyesters; D053773:Transforming Growth Factor beta1; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C033616:poly(lactide); D004815:Epidermal Growth Factor; D003474:Curcumin
keywords: EGF; curcumin; epidermal growth factor; nanoparticles; thermosensitive hydrogel; wound healing