title: Inactivation of Zaire ebolavirus Variant Makona in Human Serum Samples Analyzed by Enzyme-Linked Immunosorbent Assay.
abstract: Personnel deployed to remote areas during infectious disease outbreaks have limited access to mechanical and chemical inactivation resources. The inactivation of infectious agents present in diagnostic samples is critical to ensure the safety of personnel and the containment of the disease. We evaluated the efficacy of thermal inactivation (exposure to 56°C for 1 hour) and chemical inactivation with 0.5% Tween-20 against a high titer of Ebola virus (species Zaire ebolavirus) variant Makona in spiked human serum samples. No surviving virus was revealed by a 50% tissue culture infective dose assay after the combined treatment under laboratory conditions. In-field use of this inactivation protocol during the 2013-2016 West Africa Ebola outbreaks demonstrated readily detectable levels of immunoglobulin G and/or immunoglobulin M in human plasma samples after treatment.
authors: Applied Biosafety Research Program, Canadian Science Centre for Human and Animal Health Applied Biosafety Research Program, JC wilt Infectious Disease Research Centre.
authors: Todd
authors: T
authors: Cutts
authors: Special Pathogens, Canadian Science Centre for Human and Animal Health.
authors: Allen
authors: A
authors: Grolla
authors: Special Pathogens, Canadian Science Centre for Human and Animal Health.
authors: Shane
authors: S
authors: Jones
authors: Applied Biosafety Research Program, Canadian Science Centre for Human and Animal Health Applied Biosafety Research Program, JC wilt Infectious Disease Research Centre.
authors: Bradley W M
authors: BW
authors: Cook
authors: Special Pathogens, Canadian Science Centre for Human and Animal Health Department of Medical Microbiology.
authors: Xiangguo
authors: X
authors: Qiu
authors: Applied Biosafety Research Program, Canadian Science Centre for Human and Animal Health Applied Biosafety Research Program, JC wilt Infectious Disease Research Centre Department of Microbiology, University of Manitoba, Winnipeg, Canada.
authors: Steven S
authors: SS
authors: Theriault
mesh_terms: D000354:Africa, Western / Q000453:epidemiology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology*; D002522:Chlorocebus aethiops; D015023:Democratic Republic of the Congo / Q000453:epidemiology; D004196:Disease Outbreaks; D029043:Ebolavirus / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D006358:Hot Temperature; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D014709:Vero Cells; D038301:Virus Inactivation
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M
keywords: ELISA; Ebola; Tween-20; detergent; field; heat; human serum; inactivation