title: Hyaluronan oligosaccharides promote diabetic wound healing by increasing angiogenesis.
abstract: BACKGROUND
  Hyaluronan (also known as hyaluronic acid) oligosaccharides (O-HA) can promote angiogenesis and wound healing; however, there are few reports on whether O-HA also plays a role in healing wounds of diabetic patients.
  
  
  METHODS
  In this study, we prepared a special ointment containing a mixture of hyaluronan fragments from 2 to 10 disaccharide units and investigated its effects on healing the wounds of diabetic rats.
  
  
  RESULTS
  We found that O-HA significantly increases proliferation, migration, and tube formation of endothelial cells under high glucose conditions, and topical administration of O-HA ointment promotes wound healing by increasing angiogenesis in the wounded area of the skin. The underlying mechanisms are that O-HA increases the phosphorylation of Src and ERK, and expression of TGF beta1, thereby increasing angiogenesis.
  
  
  CONCLUSIONS
  This suggests that topical application of O-HA could be a useful method by which to treat diabetic wounds in clinical practice.
authors: Graduated School of Liaoning Medical University, Jinzhou, China; School of Pharmaceutical Science, Liaoning Medical University, Jinzhou, China.
authors: Yi
authors: Y
authors: Wang
authors: First Affiliated Hospital of Liaoning Medical University, Jinzhou, China. Electronic address: hgy19800223@163.com.
authors: Guanying
authors: G
authors: Han
authors: First Affiliated Hospital of Liaoning Medical University, Jinzhou, China.
authors: Bin
authors: B
authors: Guo
authors: First Affiliated Hospital of Liaoning Medical University, Jinzhou, China. Electronic address: hjhuadr@163.com.
authors: Jianhua
authors: J
authors: Huang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000473:pathology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000502:physiology; D009824:Ointments / Q000008:administration & dosage; D009844:Oligosaccharides / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D009824:Ointments; D009844:Oligosaccharides; D006820:Hyaluronic Acid
keywords: Angiogenesis; Diabetic wound healing; Hyaluronan oligosaccharides