title: Sympathetic cardiac function in early sepsis: Noninvasive evaluation with [123I]-meta-iodobenzylguanidine (123I-MIBG) in vivo SPECT imaging.
abstract: Sympathetic system abnormalities have been reported in sepsis-related cardiac dysfunction. The present study aimed at evaluating the potential of the norepinephrine radiolabeled analogue [123I]-meta-iodobenzylguanidine (123I-MIBG) for the noninvasive assessment of modifications in cardiac sympathetic activity occurring in lipopolysaccharide (LPS)-induced experimental acute sepsis by single-photon emission computed tomographic imaging (SPECT).
  
  
  
  Sepsis was induced in male Wistar rats by intraperitoneal injection of 10 mg·kg-1lipopolysaccharide (n = 16), whereas control animals (n = 7) were injected with vehicle (NaCl 0.9%). Echocardiography in LPS-injected animals (n = 8) demonstrated systolic and diastolic cardiac dysfunction.123I-MIBG was injected 1 hour after LPS or vehicle administration (n = 8 and 7, respectively), and in vivo SPECT imaging was performed early and late (20 and 180 minutes) after tracer injection prior to animal euthanasia and ex vivo assessment of123I-MIBG biodistribution. Global and 17-segment SPECT image analysis indicated that early123I-MIBG activity was not affected by LPS treatment, whereas late cardiac tracer activity was significantly decreased in LPS-treated animals. Consequently, the cardiac washout of123I-MIBG was significantly higher in LPS-treated (63.3% ± 4.0%) than that in control animals (56.7% ± 5.8%) (P < .05).
  
  
  
  Sepsis-induced modifications in cardiac sympathetic nervous system activity were evidenced by noninvasive in vivo123I-MIBG SPECT imaging.
authors: INSERM U1039, Radiopharmaceutiques Biocliniques, Grenoble, France.|Université Grenoble Alpes, Grenoble, France.
authors: Romain
authors: R
authors: Clerc
authors: INSERM U1039, Radiopharmaceutiques Biocliniques, Grenoble, France.|Université Grenoble Alpes, Grenoble, France.
authors: Sophia
authors: S
authors: Doll
authors: INSERM U1039, Radiopharmaceutiques Biocliniques, Grenoble, France. Laurent.Riou@univ-grenoble-alpes.fr.|Université Grenoble Alpes, Grenoble, France. Laurent.Riou@univ-grenoble-alpes.fr.|INSERM U1039, Faculté de Médecine de Grenoble, 38700, La Tronche, France. Laurent.Riou@univ-grenoble-alpes.fr.
authors: Laurent M
authors: LM
authors: Riou
authors: INSERM U1039, Radiopharmaceutiques Biocliniques, Grenoble, France.|Université Grenoble Alpes, Grenoble, France.
authors: Pascale
authors: P
authors: Perret
authors: INSERM U1039, Radiopharmaceutiques Biocliniques, Grenoble, France.|Université Grenoble Alpes, Grenoble, France.
authors: Alexis
authors: A
authors: Broisat
authors: INSERM U1039, Radiopharmaceutiques Biocliniques, Grenoble, France.|Université Grenoble Alpes, Grenoble, France.
authors: Audrey
authors: A
authors: Soubies
authors: INSERM U1039, Radiopharmaceutiques Biocliniques, Grenoble, France.|Nuclear Medicine Department, Grenoble University Hospital, Grenoble, France.
authors: Marie-Dominique
authors: MD
authors: Desruet
authors: INSERM U1039, Radiopharmaceutiques Biocliniques, Grenoble, France.|Nuclear Medicine Department, Grenoble University Hospital, Grenoble, France.
authors: Daniel
authors: D
authors: Fagret
authors: INSERM U1039, Radiopharmaceutiques Biocliniques, Grenoble, France.|Université Grenoble Alpes, Grenoble, France.|Intensive Care Unit, Grenoble University Hospital, Grenoble, France.
authors: Carole
authors: C
authors: Schwebel
authors: INSERM U1039, Radiopharmaceutiques Biocliniques, Grenoble, France.|Université Grenoble Alpes, Grenoble, France.
authors: Catherine
authors: C
authors: Ghezzi
mesh_terms: D019797:3-Iodobenzylguanidine / Q000493:pharmacokinetics*; D000818:Animals; D006321:Heart / Q000000981:diagnostic imaging*; D008070:Lipopolysaccharides / Q000737:chemistry; D008297:Male; D009638:Norepinephrine / Q000097:blood; D011379:Prognosis; D051381:Rats; D017208:Rats, Wistar; D011941:Receptors, Adrenergic / Q000378:metabolism; D012772:Shock, Septic / Q000503:physiopathology*; D013564:Sympathetic Nervous System / Q000503:physiopathology*; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D008070:Lipopolysaccharides; D011941:Receptors, Adrenergic; D019797:3-Iodobenzylguanidine; D009638:Norepinephrine
keywords: Basic science; MIBG imaging; experimental sepsis; microSPECT imaging