title: A R2R3-MYB transcription factor from Lablab purpureus induced by drought increases tolerance to abiotic stress in Arabidopsis.
abstract: Few regulators for drought tolerance have been identified in Lablab purpureus which is a multipurpose leguminous crop. The transcription factor MYB is involved in regulatory networks in response to abiotic and biotic stresses in plants. A novel R2R3-MYB factor in L. purpureus has been identified. An suppression subtraction hybridization (SSH) library was constructed using root tissues of L. purpureus MEIDOU 2012 from well-watered and water-stress treatments that were subjected to drought stress for 10 days. In addition, the cDNA of LpMYB1 was identified based on the SSH library. The cDNA of LpMYB1 is 858 bp and encodes a 285-amino acid protein with a calculated mass of 33.4 kDa. The LpMYB1 protein localizes to the nucleus and has transactivation activity with the activation domain in the C terminal region of the protein. In LpMYB1 overexpressed Arabidopsis, the tolerance of transgenic seedlings to drought and salt was improved, and the germination potential of transgenic seeds increase in the presence of NaCl or ABA. LpMYB1 is a drought-responsive R2R3-MYB factor that can increase the drought and salt tolerance of LpMYB1-overexpressed Arabidopsis.
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors: Luming
authors: L
authors: Yao
authors: Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
authors: Yina
authors: Y
authors: Jiang
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors: Xinxin
authors: X
authors: Lu
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China. wangbiao@sjtu.edu.cn.
authors: Biao
authors: B
authors: Wang
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors: Pei
authors: P
authors: Zhou
authors: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors: Tianlong
authors: T
authors: Wu
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D002467:Cell Nucleus / Q000378:metabolism; D055864:Droughts; D007887:Fabaceae / Q000235:genetics / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant; D018525:Germination; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics; D030821:Plants, Genetically Modified / Q000254:growth & development; D055049:Salt Tolerance; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: Abiotic stress; Arabidopsis thaliana; Lablab purpureus; MYB factor