title: Correlation of Tumor Immunohistochemistry with Dynamic Contrast-Enhanced and DSC-MRI Parameters in Patients with Gliomas.
abstract: Tumor CBV is a prognostic and predictive marker for patients with gliomas. Tumor CBV can be measured noninvasively with different MR imaging techniques; however, it is not clear which of these techniques most closely reflects histologically-measured tumor CBV. Our aim was to investigate the correlations between dynamic contrast-enhanced and DSC-MR imaging parameters and immunohistochemistry in patients with gliomas.
  
  
  
  Forty-three patients with a new diagnosis of glioma underwent a preoperative MR imaging examination with dynamic contrast-enhanced and DSC sequences. Unnormalized and normalized cerebral blood volume was obtained from DSC MR imaging. Two sets of plasma volume and volume transfer constant maps were obtained from dynamic contrast-enhanced MR imaging. Plasma volume obtained from the phase-derived vascular input function and bookend T1 mapping (Vp_Φ) and volume transfer constant obtained from phase-derived vascular input function and bookend T1 mapping (Ktrans_Φ) were determined. Plasma volume obtained from magnitude-derived vascular input function (Vp_SI) and volume transfer constant obtained from magnitude-derived vascular input function (Ktrans_SI) were acquired, without T1 mapping. Using CD34 staining, we measured microvessel density and microvessel area within 3 representative areas of the resected tumor specimen. The Mann-Whitney U test was used to test for differences according to grade and degree of enhancement. The Spearman correlation was performed to determine the relationship between dynamic contrast-enhanced and DSC parameters and histopathologic measurements.
  
  
  
  Microvessel area, microvessel density, dynamic contrast-enhanced, and DSC-MR imaging parameters varied according to the grade and degree of enhancement (P < .05). A strong correlation was found between microvessel area and Vp_Φ and between microvessel area and unnormalized blood volume (rs≥ 0.61). A moderate correlation was found between microvessel area and normalized blood volume, microvessel area and Vp_SI, microvessel area and Ktrans_Φ, microvessel area and Ktrans_SI, microvessel density and Vp_Φ, microvessel density and unnormalized blood volume, and microvessel density and normalized blood volume (0.44 ≤ rs≤ 0.57). A weaker correlation was found between microvessel density and Ktrans_Φ and between microvessel density and Ktrans_SI (rs≤ 0.41).
  
  
  
  With dynamic contrast-enhanced MR imaging, use of a phase-derived vascular input function and bookend T1 mapping improves the correlation between immunohistochemistry and plasma volume, but not between immunohistochemistry and the volume transfer constant. With DSC-MR imaging, normalization of tumor CBV could decrease the correlation with microvessel area.
authors: From the Departments of Radiology (T.B.N., G.O.C., C.H.T., R.E.T., I.G.C., S.C.) thnguyen@toh.on.ca.
authors: T B
authors: 0000-0001-8458-3351
authors: TB
authors: Nguyen
authors: From the Departments of Radiology (T.B.N., G.O.C., C.H.T., R.E.T., I.G.C., S.C.).
authors: G O
authors: 0000-0003-1117-9678
authors: GO
authors: Cron
authors: Psychiatry (K.B.), The Ottawa Hospital, University of Ottawa, The Ottawa Hospital Research Institute, Ottawa, Ontario, Canada.
authors: K
authors: 0000-0003-1667-3296
authors: K
authors: Bezzina
authors: Internal Medicine (K.P.).
authors: K
authors: 0000-0002-8746-665X
authors: K
authors: Perdrizet
authors: From the Departments of Radiology (T.B.N., G.O.C., C.H.T., R.E.T., I.G.C., S.C.).
authors: C H
authors: 0000-0003-4104-7115
authors: CH
authors: Torres
authors: From the Departments of Radiology (T.B.N., G.O.C., C.H.T., R.E.T., I.G.C., S.C.).
authors: S
authors: 0000-0002-2975-2260
authors: S
authors: Chakraborty
authors: Pathology (G.H.J., J.W.).
authors: J
authors: 0000-0002-7382-6343
authors: J
authors: Woulfe
authors: Pathology (G.H.J., J.W.).
authors: G H
authors: 0000-0002-4316-7666
authors: GH
authors: Jansen
authors: From the Departments of Radiology (T.B.N., G.O.C., C.H.T., R.E.T., I.G.C., S.C.).
authors: R E
authors: 0000-0001-5308-5106
authors: RE
authors: Thornhill
authors: Department of Medical Biophysics (B.Z.), University of Toronto, Toronto, Ontario, Canada.
authors: B
authors: 0000-0001-5645-0180
authors: B
authors: Zanette
authors: From the Departments of Radiology (T.B.N., G.O.C., C.H.T., R.E.T., I.G.C., S.C.).|Medical Physics (I.G.C.).
authors: I G
authors: 0000-0002-0464-8790
authors: IG
authors: Cameron
mesh_terms: D000328:Adult; D000465:Algorithms; D001810:Blood Volume; D001932:Brain Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000503:physiopathology; D003287:Contrast Media; D005260:Female; D005910:Glioma / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000503:physiopathology; D006801:Humans; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D055806:Microvessels / Q000000981:diagnostic imaging / Q000473:pathology; D008875:Middle Aged; D011379:Prognosis; D018709:Statistics, Nonparametric
chemical_list: D003287:Contrast Media