title: Influence of soil contamination with PAH on microbial community dynamics and expression level of genes responsible for biodegradation of PAH and production of rhamnolipids.
abstract: The aim of this study was to evaluate the effect of bioaugmentation and addition of rhamnolipids on the biodegradation of PAHs in artificially contaminated soil, expression of genes crucial for the biodegradation process (PAHRHDαGN, PAHRHDαGP), and the synthesis of rhamnolipids as well as population changes in the soil bacterial metabiome. The positive effect of bioaugmentation and addition of rhamnolipids on the bioremediation of the majority of PAHs was confirmed during the early stages of treatment, especially in case of the most structurally complicated compounds. The results of metagenomic analysis indicated that the initial changes in the soil metabiome caused by bioaugmentation diminished after 3 months and that the community structure in treated soil was similar to control. The survival period of bacteria introduced into the soil via bioaugmentation reached a maximum of 3 months. The increased expression of genes observed after addition of PAH into the soil also returned to the initial conditions after 3 months.
authors: Institute of Food Technology of Plant Origin, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624, Poznań, Poland.
authors: Zuzanna
authors: Z
authors: Szczepaniak
authors: Institute of Forensic Genetics, Al. Mickiewicza 3/4, 85-071, Bydgoszcz, Poland.
authors: Jakub
authors: J
authors: Czarny
authors: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627, Poznań, Poland.
authors: Justyna
authors: J
authors: Staninska-Pięta
authors: Institute of Food Technology of Plant Origin, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624, Poznań, Poland.
authors: Piotr
authors: P
authors: Lisiecki
authors: Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60-965, Poznań, Poland.
authors: Agnieszka
authors: A
authors: Zgoła-Grześkowiak
authors: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627, Poznań, Poland.
authors: Paweł
authors: P
authors: Cyplik
authors: Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60-965, Poznań, Poland.
authors: Łukasz
authors: Ł
authors: Chrzanowski
authors: Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632, Poznań, Poland.
authors: Łukasz
authors: Ł
authors: Wolko
authors: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627, Poznań, Poland.
authors: Roman
authors: R
authors: Marecik
authors: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627, Poznań, Poland.
authors: Wojciech
authors: W
authors: Juzwa
authors: Department of Forest Technology, Poznan University of Life Sciences, 71C Wojska Polskiego St., 60-625, Poznan, Poland.
authors: Katarzyna
authors: K
authors: Glazar
authors: Institute of Food Technology of Plant Origin, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624, Poznań, Poland. apio@up.poznan.pl.
authors: Agnieszka
authors: A
authors: Piotrowska-Cyplik
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D015870:Gene Expression; D006017:Glycolipids / Q000378:metabolism*; D059013:Microbial Consortia / Q000187:drug effects*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism / Q000633:toxicity*; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D006017:Glycolipids; D011084:Polycyclic Aromatic Hydrocarbons; D012987:Soil; D012989:Soil Pollutants; C418382:rhamnolipid
keywords: Bioaugmentation; Dioxygenase; MiSeq technology; PAHs; Rhamnolipids; Rhamnosylotransferase