title: The Genome-Wide Analysis of Carcinoembryonic Antigen Signaling by Colorectal Cancer Cells Using RNA Sequencing.
abstract: Сarcinoembryonic antigen (CEA, CEACAM5, CD66) is a promoter of metastasis in epithelial cancers that is widely used as a prognostic clinical marker of metastasis. The aim of this study is to identify the network of genes that are associated with CEA-induced colorectal cancer liver metastasis. We compared the genome-wide transcriptomic profiles of CEA positive (MIP101 clone 8) and CEA negative (MIP 101) colorectal cancer cell lines with different metastatic potential in vivo. The CEA-producing cells displayed quantitative changes in the level of expression for 100 genes (over-expressed or down-regulated). They were confirmed by quantitative RT-PCR. The KEGG pathway analysis identified 4 significantly enriched pathways: cytokine-cytokine receptor interaction, MAPK signaling pathway, TGF-beta signaling pathway and pyrimidine metabolism. Our results suggest that CEA production by colorectal cancer cells triggers colorectal cancer progression by inducing the epithelial- mesenchymal transition, increasing tumor cell invasiveness into the surrounding tissues and suppressing stress and apoptotic signaling. The novel gene expression distinctions establish the relationships between the existing cancer markers and implicate new potential biomarkers for colorectal cancer hepatic metastasis.
authors: Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, St. Petersburg, Russia.|Department of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia.
authors: Olga
authors: http://orcid.org/0000-0001-7506-4736
authors: O
authors: Bajenova
authors: Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, St. Petersburg, Russia.
authors: Anna
authors: A
authors: Gorbunova
authors: Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, St. Petersburg, Russia.
authors: Igor
authors: I
authors: Evsyukov
authors: Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, St. Petersburg, Russia.
authors: Michael
authors: M
authors: Rayko
authors: Boston Children's Hospital, Boston, MA, United States of America.
authors: Svetlana
authors: S
authors: Gapon
authors: Institute of Cytology RAS, St. Petersburg, Russia.
authors: Ekaterina
authors: E
authors: Bozhokina
authors: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, United States of America.
authors: Alexander
authors: A
authors: Shishkin
authors: Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University, St. Petersburg, Russia.|Oceanographic Center, 8000 N. Ocean Drive, Nova Southeastern University, Ft Lauderdale, Florida, 33004, United States of America.
authors: Stephen J
authors: SJ
authors: O'Brien
mesh_terms: D002272:Carcinoembryonic Antigen / Q000378:metabolism*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000276:immunology; D020869:Gene Expression Profiling; D055106:Genome-Wide Association Study; D006801:Humans; D016133:Polymerase Chain Reaction; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D002272:Carcinoembryonic Antigen