title: Comparative immunohistochemical assessment of the effect of repetitive anesthesia with isoflurane and sevoflurane on rat liver.
abstract: OBJECTIVE
  Inhalation anesthetics are used in human, as well as veterinary medical practice. In the present study we investigated the effect of isoflurane and sevoflurane on rat hepatocytes.
  
  
  METHODS
  A total of 40 Wistar female rats were used in this study. Animals were divided in groups of 5 rats. Groups IM, SM served as control groups. Groups I1, I2, I3 were used to study isoflurane and S1, S2, S3 for sevoflurane study. They were anesthetized 3 times, for 2h long, at 2 days interval with a concentration of: 1.5% isoflurane (I1, I2, I3) and 2% sevoflurane (S1, S2, S3). The oxygen supply throughout the anesthesia was 1LO2/min. Groups IM, IS, I1, S1 were sacrificed immediately after the last anesthesia. Groups I2, S2 were sacrificed 6h after the last anesthesia, and groups I3, S3, 24h post-anesthesia. Liver samples were harvested to highlight caspase-3 in apoptotic hepatocytes.
  
  
  RESULTS
  Following isoflurane administration, there were less than 1% cells in apoptosis highlighted in rat livers from groups IM, I1 and I2. At 24h post-anesthesia (group I3), a small number of apoptotic hepatocytes was highlighted (around 3.23% cells in apoptosis), with a strictly periacinar disposition, randomly distributed in a small number of hepatic lobules. After sevoflurane administration, less than 1% apoptotic hepatocytes were identified at all control moments throughout the study.
  
  
  CONCLUSIONS
  The results suggest that the anesthetics do not present a considerable hepatotoxicity. The comparative assessment of the two anesthetics shows that sevoflurane is superior to isoflurane.
authors: Department of Cell Biology, Histology and Embriology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania.
authors: Flavia
authors: F
authors: Ruxanda
authors: Department of Pathological Anatomy, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania. Electronic address: adrianfloringal@yahoo.com.
authors: Adrian Florin
authors: AF
authors: Gal
authors: Department of Implantology, Faculty of Medicine and Pharmacy, University of Oradea, Oradea, Romania.
authors: Cristian
authors: C
authors: Raţiu
authors: Department of Cell Biology, Histology and Embriology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania.
authors: Viorel
authors: V
authors: Miclăuş
authors: Department of Cell Biology, Histology and Embriology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania.
authors: Vasile
authors: V
authors: Rus
authors: Department of Anesthesiology and Surgical Propedeutics, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania.
authors: Liviu Ioan
authors: LI
authors: Oana
mesh_terms: D018685:Anesthetics, Inhalation / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D007150:Immunohistochemistry; D007530:Isoflurane / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008738:Methyl Ethers / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D000077149:Sevoflurane; D007530:Isoflurane
keywords: Anestesia; Anesthesia; Caspase-3; Fígado; Isoflurane; Isoflurano; Liver; Sevoflurane; Sevoflurano