title: Streptomyces tremellae sp. nov., isolated from a culture of the mushroom Tremella fuciformis.
abstract: A novel actinomycete strain, designated Js-1T, was isolated from Tremella fuciformis collected from Gutian, Fujian Province, in southeastern China. The taxonomic status of this strain was determined by a polyphasic approach, which demonstrated that the novel strain was a member of the genus Streptomyces. The cell walls of this strain were found to contain ll-diaminopimelic acid, muramic acid and glycine. An analysis of whole-cell hydrolysates revealed that no characteristic sugar was present. The key identified menaquinones were MK-9 (H6) and MK-9 (H8), while the diagnostic polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylmethylethanolamine and phosphatidylglycerol. The main cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, C16 : 0 and iso-C16 : 0. An analysis of an almost complete 16S rRNA gene sequence showed that the strain shared the highest levels of sequence similarity with Streptomyces sannanensisKC-7038T (97.87 %), Streptomyces hebeiensis YIM 001T (97.84 %), Streptomyces pathocidini NBRC 13812T (97.80 %), Streptomyces cocklensis BK168T (97.25 %), Streptomyces coerulescens NBRC 12758T (97.12 %), Streptomyces aurantiogriseus NBRC 12842T (97.06 %) and Streptomyces rimosussubsp. rimosus ATCC 10970T (97.04 %). The DNA G+C content of the genomic DNA of strain Js-1T was 70.1 mol%. Furthermore, DNA-DNA hybridization tests revealed that the relatedness values between strain Js-1T and the most closely related species ranged from 15.10 to 47.20 %. Based on its phenotypic and genotypic characteristics, strain Js-1T (=CCTCC M 2011365T=JCM 30846T) is considered to represent a novel species within the genus Streptomyces, which we classified as Streptomycestremellae sp. nov.
authors: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350028, PR China.
authors: Zhi-Qiang
authors: ZQ
authors: Wen
authors: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350028, PR China.
authors: Bingzhi
authors: B
authors: Chen
authors: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350028, PR China.
authors: Xiao
authors: X
authors: Li
authors: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350028, PR China.|Huaian Center for Disease Control and Prevention, Huaian, Jiangsu 223001, PR China.
authors: Bing-Bing
authors: BB
authors: Li
authors: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350028, PR China.
authors: Cheng-Huan
authors: CH
authors: Li
authors: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350028, PR China.
authors: Qing-Hua
authors: QH
authors: Huang
authors: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350028, PR China.
authors: Qi-Hui
authors: QH
authors: Zhang
authors: Xinghua Fungus Institute, Gutian County, Sanming, Fujian 352259, PR China.
authors: Wei-Hao
authors: WH
authors: Dai
authors: Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350028, PR China.
authors: Yu-Ji
authors: YJ
authors: Jiang
mesh_terms: D000363:Agaricales; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001487:Basidiomycota; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid