title: Dual effects of a high-protein diet on DSS-treated mice during colitis resolution phase.
abstract: The impact of the dietary protein level on the process of colonic mucosal inflammation and subsequent recovery remains largely unknown. In this study, we fed DSS-treated mice with either a normoproteic (NP) or a high-protein (HP) isocaloric diet from the beginning of the 5-day dextran sulfate sodium (DSS) treatment to 14 days later. Measurements of colitis indicators (colon weight:length ratio, myeloperoxidase activity, cytokine expressions) showed a similar level of colonic inflammation in both DSS groups during the colitis induction phase. However, during the colitis resolution phase, inflammation intensity was higher in the DSS-HP group than in the DSS-NP group as evidenced by higher inflammatory score and body weight loss. This coincided with a higher mortality rate. In surviving animals, an increase in colonic crypt height associated with a higher number of colon epithelial cells per crypt, and TGF-β3 content was observed in the DSS-HP vs. DSS-NP group. Moreover, colonic expression patterns of tight junction proteins and E-cadherin were also different according to the diet. Altogether, our results indicate that the HP diet, when given during both the induction and resolution periods of DSS-induced colitis, showed deleterious effects during the post-induction phase. However, HP diet ingestion was also associated with morphological and biochemical differences compatible with higher colonic epithelium restoration in surviving animals, indicating an effect of the dietary protein level on colonic crypt repair after acute inflammation. These data highlight the potential impact of the dietary protein amount during the colitis course.
authors: UMR PNCA, Nutrition Physiology and Ingestive Behavior, AgroParisTech, INRA, Université Paris-Saclay, Paris, France; annaig.lan@agroparistech.fr.
authors: Annaïg
authors: A
authors: Lan
authors: UMR PNCA, Nutrition Physiology and Ingestive Behavior, AgroParisTech, INRA, Université Paris-Saclay, Paris, France.
authors: Anne
authors: A
authors: Blais
authors: Laboratory of Applied Nutrition and Metabolism, School of Physical Education and Sports, University of São Paulo, Brazil.
authors: Desire
authors: D
authors: Coelho
authors: UMR PNCA, Nutrition Physiology and Ingestive Behavior, AgroParisTech, INRA, Université Paris-Saclay, Paris, France.
authors: Juliette
authors: J
authors: Capron
authors: UMR PNCA, Nutrition Physiology and Ingestive Behavior, AgroParisTech, INRA, Université Paris-Saclay, Paris, France.
authors: Manar
authors: M
authors: Maarouf
authors: UMR PNCA, Nutrition Physiology and Ingestive Behavior, AgroParisTech, INRA, Université Paris-Saclay, Paris, France; Department of Gastroenterology, Hôpital Avicenne, Université Paris 13, Bobigny, France; and.
authors: Robert
authors: R
authors: Benamouzig
authors: Laboratory of Applied Nutrition and Metabolism, School of Physical Education and Sports, University of São Paulo, Brazil.
authors: Antonio H
authors: AH
authors: Lancha
authors: Service d'Anatomie et Cytologie Pathologiques, Hôpital Bichat-Claude Bernard, Paris, France.
authors: Francine
authors: F
authors: Walker
authors: UMR PNCA, Nutrition Physiology and Ingestive Behavior, AgroParisTech, INRA, Université Paris-Saclay, Paris, France.
authors: Daniel
authors: D
authors: Tomé
authors: UMR PNCA, Nutrition Physiology and Ingestive Behavior, AgroParisTech, INRA, Université Paris-Saclay, Paris, France.
authors: François
authors: F
authors: Blachier
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism; D003106:Colon / Q000187:drug effects* / Q000378:metabolism; D016264:Dextran Sulfate; D004044:Dietary Proteins / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000178:diet therapy / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D053782:Transforming Growth Factor beta3 / Q000378:metabolism
chemical_list: D004044:Dietary Proteins; D053782:Transforming Growth Factor beta3; D016264:Dextran Sulfate
keywords: DSS; colitis induction; colitis resolution; colonic crypt repair; high-protein diet