title: Polysaccharides purified from Cordyceps cicadae protects PC12 cells against glutamate-induced oxidative damage.
abstract: Two polysaccharides CPA-1 and CPB-2 were isolated purified from Cordyceps cicadae by hot water extraction, ethanol precipitation and purification using anion exchange and gel filtration chromatography. Preliminary structural characterization of CPA-1 and CPB-2 were performed. The protective effect of CPA-1 and CPB-2 against glutamate-induced oxidative toxicity in PC12 cells was analyzed. The results indicated that pretreatment of PC12 cells with CPA-1 and CPB-2 significantly increased cell survival, Ca(2+) overload and ROS generation. CPA-1 and CPB-2 also markedly up-regulated the antioxidant status of pretreated PC12 cells. Our results suggested that Cordyceps cicadae polysaccharides can protect PC12 cells against glutamate excitotoxicity and might serve as therapeutic agents for neuronal disorders.
authors: School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China.
authors: Opeyemi J
authors: OJ
authors: Olatunji
authors: School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China.
authors: Yan
authors: Y
authors: Feng
authors: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences Prince of Songkla University, Hat Yai, 90112, Thailand.
authors: Oyenike O
authors: OO
authors: Olatunji
authors: School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China.
authors: Jian
authors: J
authors: Tang
authors: School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China.
authors: Yuan
authors: Y
authors: Wei
authors: School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China. Electronic address: zhenouyang@ujs.edu.cn.
authors: Zhen
authors: Z
authors: Ouyang
authors: School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, 212013, China.
authors: Zhaoliang
authors: Z
authors: Su
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000032:analysis; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D032661:Cordyceps / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000494:pharmacology; D008516:Medicine, Chinese Traditional; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000032:analysis
chemical_list: D018696:Neuroprotective Agents; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D018698:Glutamic Acid; D002118:Calcium
keywords: Cordyceps cicadae; Glutamate; Neuroprotection; PC12 cells; Polysaccharide