title: Ingenine E, a new cytotoxic β-carboline alkaloid from the Indonesian sponge Acanthostrongylophora ingens.
abstract: A new β-carboline alkaloid ingenine E (4), along with three known metabolites annomontine (1), acanthomine A (2), and 1,2,3,4-tetrahydronorharman-1-one (3) were isolated from the Indonesian marine sponge Acanthostrongylophora ingens. Their structure characterization was unequivocally carried out using one- and two-dimensional NMR spectroscopy, as well as high-resolution mass spectrometry. The cytotoxic activity of the isolated compounds was evaluated toward MCF7, A549, HCT116, PC12, and Hela cancer cell lines. Ingenine E (4) exhibited cytotoxic activity against MCF7, HCT116, and A549 cell lines with IC50values of 3.5, 0.67, and 2.15 μg/ml.
authors: a Department of Pharmacognosy and Pharmaceutical Chemistry , College of Pharmacy, Taibah University , Al Madinah Al Munawwarah 30078 , Saudi Arabia.|b Faculty of Pharmacy, Department of Pharmacognosy , Assiut University , Assiut 71526 , Egypt.
authors: Sabrin R M
authors: SR
authors: Ibrahim
authors: c Faculty of Pharmacy, Department of Natural Products and Alternative Medicine , King Abdulaziz University , Jeddah 21589 , Saudi Arabia.|d Faculty of Pharmacy, Department of Pharmacognosy , Al-Azhar University, Assiut Branch , Assiut 71524 , Egypt.
authors: Gamal A
authors: GA
authors: Mohamed
mesh_terms: D000470:Alkaloids / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002227:Carbazoles / Q000737:chemistry; D002243:Carbolines / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D045325:HCT116 Cells; D006367:HeLa Cells; D006801:Humans; D007214:Indonesia; D008386:Marine Biology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D016716:PC12 Cells; D011161:Porifera / Q000737:chemistry*; D011743:Pyrimidines; D051381:Rats
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D002227:Carbazoles; D002243:Carbolines; D011743:Pyrimidines; C508838:annomontine; C000617259:ingenine E; C010262:norharman
keywords: Acanthostrongylophora ingens; alkaloids; cytotoxic activity; ingenine E; β-carboline