title: Transcriptional Induction of Metallothionein by Tris(pentafluorophenyl)stibane in Cultured Bovine Aortic Endothelial Cells.
abstract: Vascular endothelial cells cover the luminal surface of blood vessels and contribute to the prevention of vascular disorders such as atherosclerosis. Metallothionein (MT) is a low molecular weight, cysteine-rich, metal-binding, inducible protein, which protects cells from the toxicity of heavy metals and active oxygen species. Endothelial MT is not induced by inorganic zinc. Adequate tools are required to investigate the mechanisms underlying endothelial MT induction. In the present study, we found that an organoantimony compound, tris(pentafluorophenyl)stibane, induces gene expression of MT-1A and MT-2A, which are subisoforms of MT in bovine aortic endothelial cells. The data reveal that MT-1A is induced by activation of both the MTF-1-MRE and Nrf2-ARE pathways, whereas MT-2A expression requires only activation of the MTF-1-MRE pathway. The present data suggest that the original role of MT-1 is to protect cells from heavy metal toxicity and oxidative stress in the biological defense system, while that of MT-2 is to regulate intracellular zinc metabolism.
authors: Department of Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, Japan. t-fujie@phar.toho-u.ac.jp.
authors: Tomoya
authors: T
authors: Fujie
authors: Department of Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, Japan. j3b13674@ed.tus.ac.jp.
authors: Masaki
authors: M
authors: Murakami
authors: Department of Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, Japan. eyoshida@rs.tus.ac.jp.
authors: Eiko
authors: E
authors: Yoshida
authors: Laboratory of Organic and Medicinal Chemistry, School of Pharmaceutical Sciences, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan. s-yasuik@dpc.agu.ac.jp.
authors: Shuji
authors: S
authors: Yasuike
authors: Depertment of Life Science, Faculty of Science and Engineering, Setsunan University, 17-8 Ikedanakamachi, Neyagawa 572-8508, Japan. tomoki@lif.setsunan.ac.jp.
authors: Tomoki
authors: T
authors: Kimura
authors: Department of Environmental Health, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji 192-0392, Japan. yasuyuki@toyaku.ac.jp.
authors: Yasuyuki
authors: Y
authors: Fujiwara
authors: Department of Environmental Health, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi 274-8510, Japan. yamamoto@phar.toho-u.ac.jp.
authors: Chika
authors: C
authors: Yamamoto
authors: Department of Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, Japan. t-kaji@rs.tus.ac.jp.
authors: Toshiyuki
authors: T
authors: Kaji
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology*; D002417:Cattle; D004268:DNA-Binding Proteins / Q000235:genetics; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D005721:Glutamate-Cysteine Ligase / Q000235:genetics; D051547:Heme Oxygenase-1 / Q000235:genetics; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology*; D008668:Metallothionein / Q000235:genetics*; D051267:NF-E2-Related Factor 2 / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D000097809:Transcription Factor MTF-1
chemical_list: D004268:DNA-Binding Proteins; D005721:Glutamate-Cysteine Ligase; D051547:Heme Oxygenase-1; D006843:Hydrocarbons, Chlorinated; D008668:Metallothionein; D051267:NF-E2-Related Factor 2; D020033:Protein Isoforms; D014157:Transcription Factors; D000097809:Transcription Factor MTF-1
keywords: bio-organometallics; metallothionein; organic–inorganic hybrid molecule; organoantimony compound; vascular endothelial cell