title: Latitudinal distribution of microbial communities in anaerobic biological stabilization ponds: effect of the mean annual temperature.
abstract: Considering wide utilization and high methane fluxes from anaerobic biological stabilization ponds (ABSPs), understanding the methanogenesis in ABSPs is of fundamental importance. Here we investigated the variation and impact factors of methanogenesis in seven ABSPs that spanned from the north to the south of China. Results showed that methanogen abundance (7.7 × 109-8.7 × 1010copies g-1dry sediment) and methanogenic activities (2.2-21.2 μmol CH4g-1dry sediment h-1) were considerable for all sediments. Statistical analysis demonstrated that compared with other factors (ammonium, pH, COD and TOC), mean annual temperature (MAT) showed the lowest P value and thus was the most important influencing factor for the methanogenic process. Besides, with the increasing MAT, methanogenic activity was enhanced mainly due to the shift of the dominant methanogenic pathway from acetoclastic (49.8-70.7%) in low MAT areas to hydrogenotrophic (42.0-54.6%) in high MAT areas. This shift of methanogenic pathway was also paralleled with changes in composition of bacterial communities. These results suggested that future global warming may reshape the composition of methanogen communities and lead to an increasing methane emission from ABSPs. Therefore, further research is urgently needed to globally estimate methane emissions from ABSPs and re-examine the role of ABSPs in wastewater treatment.
authors: Institute of Environmental Science and Technology, Zhejiang University, Hangzhou, 310058, China.
authors: Mengdong
authors: M
authors: Yuan
authors: Institute of Environmental Science and Technology, Zhejiang University, Hangzhou, 310058, China.
authors: Jing
authors: J
authors: Zhu
authors: Institute of Environmental Science and Technology, Zhejiang University, Hangzhou, 310058, China.
authors: Cheng
authors: C
authors: Wang
authors: Institute of Environmental Science and Technology, Zhejiang University, Hangzhou, 310058, China.
authors: Mengxiong
authors: M
authors: Wu
authors: Institute of Environmental Science and Technology, Zhejiang University, Hangzhou, 310058, China.
authors: Faqian
authors: F
authors: Sun
authors: Institute of Environmental Science and Technology, Zhejiang University, Hangzhou, 310058, China.
authors: Xingguo
authors: X
authors: Han
authors: Institute of Environmental Science and Technology, Zhejiang University, Hangzhou, 310058, China.
authors: Yangyang
authors: Y
authors: He
authors: Institute of Environmental Science and Technology, Zhejiang University, Hangzhou, 310058, China. weixiang@zju.edu.cn.
authors: Weixiang
authors: W
authors: Wu
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D058448:Biota; D002681:China; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008697:Methane / Q000378:metabolism*; D010802:Phylogeny; D061005:Ponds / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D008697:Methane