title: MicroRNA-195 targets VEGFR2 and has a tumor suppressive role in ACHN cells via PI3K/Akt and Raf/MEK/ERK signaling pathways.
abstract: Increasing evidence indicates that dysregulation of miR-195 may contribute to the occurrence and development of multiple types of human malignancies. However, the function and the mechanism of miR-195 in clear cell renal cell carcinoma (ccRCC) are still not fully understood. In the present study, we used qRT-PCR to detect the expression of miR-195 in ccRCC tissues and normal kidney tissues. MTT assay was performed to detect the cell viability of miR-195. Migration and invasion were evaluated by Transwell migration and Matrigel invasion assays, respectively. Additionally, apoptosis levels were evaluated using TUNEL assays, and signaling pathway changes were determined by western blot analysis. We observed that miR-195 was downregulated in clear cell renal cell carcinoma samples compared with normal renal samples. We identified that overexpression of miR-195 inhibited ACHN cell viability, migration, invasion, and it also induced cell apoptosis by targeting VEGFR2 via PI3K/Akt and Raf/MEK/ERK signaling pathways. These findings indicate that miR-195 has a tumor suppressive role in ACHN cells and miR-195 may be a promising candidate target for prevention and treatment of renal cell carcinoma.
authors: Department of Urology, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors: Pengcheng
authors: P
authors: Sun
authors: Department of Urology, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors: Lu
authors: L
authors: Wang
authors: Department of Urology, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors: Yunhan
authors: Y
authors: Lu
authors: Department of Urology, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors: Yuwei
authors: Y
authors: Liu
authors: Department of Urology, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors: Lechen
authors: L
authors: Li
authors: Department of Urology, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors: Luyao
authors: L
authors: Yin
authors: Department of Urology, The First Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors: Cheng
authors: C
authors: Zhang
authors: Department of Urology, The First Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors: Weiming
authors: W
authors: Zhao
authors: Department of Radiology, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors: Baozhong
authors: B
authors: Shen
authors: Department of Urology, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors: Wanhai
authors: W
authors: Xu
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D020935:MAP Kinase Signaling System; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics*
chemical_list: C552722:MIRN195 microRNA, human; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; C000588866:KDR protein, human; D040301:Vascular Endothelial Growth Factor Receptor-2; D051057:Proto-Oncogene Proteins c-akt