title: Development and Validation of a Kit to Measure Drink Antioxidant Capacity Using a Novel Colorimeter.
abstract: Measuring the antioxidant capacity of foods is essential, as a means of quality control to ensure that the final product reaching the consumer will be of high standards. Despite the already existing assays with which the antioxidant activity is estimated, new, faster and low cost methods are always sought. Therefore, we have developed a novel colorimeter and combined it with a slightly modified DPPH assay, thus creating a kit that can assess the antioxidant capacity of liquids (e.g., different types of coffee, beer, wine, juices) in a quite fast and low cost manner. The accuracy of the colorimeter was ensured by comparing it to a fully validated Hitachi U-1900 spectrophotometer, and a coefficient was calculated to eliminate the observed differences. In addition, a new, user friendly software was developed, in order to render the procedure as easy as possible, while allowing a central monitoring of the obtained results. Overall, a novel kit was developed, with which the antioxidant activity of liquids can be measured, firstly to ensure their quality and secondly to assess the amount of antioxidants consumed with the respective food.
authors: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa 41221, Greece. alexandros.priftis@hotmail.com.
authors: Alexandros
authors: A
authors: Priftis
authors: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa 41221, Greece. stagkos@uth.gr.
authors: Dimitrios
authors: D
authors: Stagos
authors: Polytech S.A., Larissa 41222, Greece. nikos.tzioumakis@outlook.com.
authors: Nikolaos
authors: N
authors: Tzioumakis
authors: Coffee Island S.A., Patras 26334, Greece. kostas@coffeeisland.gr.
authors: Konstantinos
authors: K
authors: Konstantinopoulos
authors: Department of Agricultural Engineering Technologists, TEI of Thessaly, Larissa 41110, Greece. anastasia.pat93@hotmail.com.
authors: Anastasia
authors: A
authors: Patouna
authors: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa 41221, Greece. geopap@bio.uth.gr.
authors: Georgios E
authors: GE
authors: Papadopoulos
authors: Department of Forensic Sciences and Toxicology, Medical School, University of Crete, Heraklion 71003, Greece. aris@med.uoc.gr.
authors: Aristides
authors: A
authors: Tsatsakis
authors: Department of Biochemistry and Biotechnology, University of Thessaly, Larissa 41221, Greece. dkouret@uth.gr.
authors: Dimitrios
authors: D
authors: Kouretas
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D006801:Humans; D011933:Reagent Kits, Diagnostic
chemical_list: D000975:Antioxidants; D011933:Reagent Kits, Diagnostic
keywords: DPPH; antioxidant; beverage; colorimeter; drink; kit; quality control