title: Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells.
abstract: In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, isolated either from the small or large intestine, maintain self-renewal and multilineage differentiation potential over time. This provides investigators a tool to culture wild type or transformed intestinal epithelium, and a robust assay for stem cell tissue homeostasisin vitro.
authors: Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program, New York, USA; Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, USA.
authors: Kevin P
authors: KP
authors: O'Rourke
authors: Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, USA.
authors: Sarah
authors: S
authors: Ackerman
authors: Department of Medicine, Weill Cornell Medical College, New York, USA.
authors: Lukas E
authors: LE
authors: Dow
authors: Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, USA; Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, USA.
authors: Scott W
authors: SW
authors: Lowe