title: Potential of Ocimum basilicum L. and Salvia officinalis L. essential oils against biofilms of P. aeruginosa clinical isolates.
abstract: Biofilms are complex communities of microorganisms, responsible for more than 60% of the chronic human infections and they represent one of the leading concerns in medicine. Pseudomonas aeruginosa is human pathogenic bacteria which causes numerous diseases and is known for its ability to produce biofilm. Ocimum basilicum L. (basil) and Salvia officinalis L. (sage) are widely used plants in traditional medicine for the treatment of different conditions. Therefore, the aim of this study was to investigate the potential of basil and sage essential oils against P. aeruginosa biofilm producing strains. The efficacy of two essential oils on P. aeruginosa biofilm forming ability was determined using crystal violet method. Out of 15 strains isolated from different clinical biological samples, two were strong, 11 moderate and one weak biofilm producer. Good efficacy of sage essential oil towards strong and weak biofilm producers, but not of basil essential oil, was observed. In the case of moderate biofilm producers, 81.8% showed lower biofilm production after incubation with the sage oil, while 63.6% showed the reduction of biofilm production after basil essential oil treatment. The obtained results showed high potential of both oils for the treatment of persistent infections caused by Pseudomonas aeruginosa biofilms.
authors: University of Niš Department of Biology and Ecology, Faculty of Science and Mathematics Niš Serbia zstojanovicradic@yahoo.com.
authors: Z
authors: Z
authors: Stojanović-Radić
authors: University of Niš Department of Biology and Ecology, Faculty of Science and Mathematics Niš Serbia.
authors: M
authors: M
authors: Pejcić
authors: University of Niš Faculty of Medicine Niš Serbia.
authors: N
authors: N
authors: Stojanović
authors: Zabol University of Medical Sciences Zabol Medicinal Plants Research Center Zabol Iran.
authors: J
authors: J
authors: Sharifi-Rad
authors: University of Niš Department of Biology and Ecology, Faculty of Science and Mathematics Niš Serbia.
authors: N
authors: N
authors: Stanković
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D004423:Ear / Q000382:microbiology; D005123:Eye / Q000382:microbiology; D006801:Humans; D018649:Ocimum basilicum / Q000737:chemistry* / Q000378:metabolism; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010614:Pharynx / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000502:physiology*; D027543:Salvia officinalis / Q000737:chemistry* / Q000378:metabolism; D012867:Skin / Q000382:microbiology
chemical_list: D009822:Oils, Volatile