title: Hybrid hydrogel photonic barcodes for multiplex detection of tumor markers.
abstract: Barcodes-based suspension array have for demonstrated values in multiplex assay of tumor markers. Photonic barcodes which are encoded by their characteristic reflection peaks are the important supports for suspension array due to their stable code, low fluorescent background and high surface-volume ratio. Attempts to develop this technology tend to improve the function of the photonic barcodes. Here, we present a new type of hybrid hydrogel photonic barcodes for efficient multiplex assays. This photonic barcodes are hybrid inverse opal hydrogel composed of poly(ethylene glycol) diacrylate (PEG-DA) and agarose. The polymerized PEG-DA hydrogel could guarantee the stabilities of the inverse opal structure and its resultant code, while the agarose could offer active chemical groups for the probe immobilization and homogeneous water surrounding for the bioassay. In addition, the interconnected pores inverse opal structure could provide channels for biomolecules diffusing and reaction into the voids of barcodes. These features imparted the hybrid hydrogel photonic barcodes with limits of detection (LOD) of 0.78ng/mL for carcinoembryonic antigen (CEA) and 0.21ng/mL for α-fetoprotein (AFP), respectively. It was also demonstrated that the proposed barcodes showed acceptable accuracy and detection reproducibility, and the results were in acceptable agreement with those from common clinic method for the detections of practical clinical samples. Thus, our technique provides a new platform for simultaneous multiplex immunoassay.
authors: Department of Hematology and Oncology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
authors: Yueshuang
authors: Y
authors: Xu
authors: Department of Hematology and Oncology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
authors: Xiaoping
authors: X
authors: Zhang
authors: Department of Hematology and Oncology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China.
authors: Chengxin
authors: C
authors: Luan
authors: School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
authors: Huan
authors: H
authors: Wang
authors: Department of Hematology and Oncology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China. Electronic address: cba8888@hotmail.com.
authors: Baoan
authors: B
authors: Chen
authors: Department of Hematology and Oncology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, China; School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address: yjzhao@seu.edu.cn.
authors: Yuanjin
authors: Y
authors: Zhao
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods*; D002272:Carcinoembryonic Antigen / Q000097:blood*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D009369:Neoplasms / Q000097:blood; D011092:Polyethylene Glycols / Q000737:chemistry*; D015203:Reproducibility of Results; D012685:Sepharose / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D000509:alpha-Fetoproteins; C437167:poly(ethylene glycol)diacrylate; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D012685:Sepharose
keywords: Barcode; Colloidal crystal; Hydrogel; Multiplex assay; Tumor marker