title: A multicommutated tester of bioreactors for flow analysis.
abstract: Enzymes are often used in the modern analytical procedures allowing selective recognition and conversion of target analytes into easily detected products. In flow analysis systems, enzymes are predominantly applied in the immobilized forms as flow-through bioreactors. In this research the multicommutated flow analysis (MCFA) system for evaluation and comparison of analytical parameters of bioreactors has been developed. The MCFA manifold allows simultaneous testing up to four bioreactors, but if necessary their number can be easily increased. The system allows comparison of several parameters of tested bioreactors including activity, repeatability, reproducibility, operational and storage stability. The performance of developed bioreactor tester is presented using urea-urease model system based on plastic open-tubular bioreactor with covalently immobilized enzyme. Product of enzymatic reaction is detected using two different chemical methods and by dedicated optoelectronic ammonium detectors. Moreover, the utility of developed MCFA manifold for evaluation of other enzyme bioreactors is demonstrated.
authors: University of Warsaw, Department of Chemistry, Pasteura 1, Warsaw, 02-093, Poland. Electronic address: mpokrzywnicka@chem.uw.edu.pl.
authors: Marta
authors: M
authors: Pokrzywnicka
authors: University of Warsaw, Department of Chemistry, Pasteura 1, Warsaw, 02-093, Poland.
authors: Jacek
authors: J
authors: Kamiński
authors: University of Warsaw, Department of Chemistry, Pasteura 1, Warsaw, 02-093, Poland; University of Warsaw, MISMaP College, Banacha 2C, Warsaw, 02-097, Poland.
authors: Michał
authors: M
authors: Michalec
authors: University of Warsaw, Department of Chemistry, Pasteura 1, Warsaw, 02-093, Poland.
authors: Robert
authors: R
authors: Koncki
authors: University of Warsaw, Department of Chemistry, Pasteura 1, Warsaw, 02-093, Poland.
authors: Łukasz
authors: Ł
authors: Tymecki
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry*; D064751:Ammonium Compounds / Q000737:chemistry; D019149:Bioreactors; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D009596:Nitrophenols / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D014508:Urea / Q000737:chemistry; D014510:Urease / Q000737:chemistry*
chemical_list: D064751:Ammonium Compounds; D004800:Enzymes, Immobilized; D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; D014508:Urea; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D000469:Alkaline Phosphatase; D014510:Urease
keywords: Alkaline phosphatase; Bioreactor; Flow analysis; Glucose oxidase; Multicommutation; Urease