title: Overexpression of CXCR7 induces angiogenic capacity of human hepatocellular carcinoma cells via the AKT signaling pathway.
abstract: Angiogenesis is essential for tumor growth, especially in hepatocellular carcinoma (HCC). The hypervascularity is associated with poor prognosis and highly invasive HCC. The C‑X‑C chemokine receptor type 7 (CXCR7) has been implied overexpressed in many tumor types. Our study aimed to investigate the CXCR7 function in HCC. The tube formation, Transwell migration assay of human umbilical vein endothelial cells (HUVECs) and chicken chorioallantoic membrane (CAM) assay were used. We confirmed that CXCR7 induces angiogenic capacity. Moreover, overexpressing CXCR7 increased the phosphorylated (but not total) AKT expression in HCC cells. Furthermore, overexpressing CXCR7 increased the expression of tumor necrosis factor (TNF)‑α, interleukin (IL)‑6 and IL‑8 in HCC cells. Additionally, inhibition of AKT by LY294002 abrogated CXCR7‑induced angiogenic capacity in HCC cells. Our study suggested that CXCR7 plays an important pro‑angiogenic role in HCC via activation of the AKT pathway. So CXCR7 may be a potential target for anti‑angiogenic therapy in HCC.
authors: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors: Yuhui
authors: Y
authors: Chen
authors: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors: Fei
authors: F
authors: Teng
authors: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors: Geying
authors: G
authors: Wang
authors: Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors: Zhiyu
authors: Z
authors: Nie
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D058990:Molecular Targeted Therapy; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000096:biosynthesis* / Q000235:genetics; D054387:Receptors, CXCR / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: C501896:ACKR3 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D054387:Receptors, CXCR; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt