title: Aneuploid embryonic stem cells exhibit impaired differentiation and increased neoplastic potential.
abstract: Aneuploidy leads to severe developmental defects in mammals and is also a hallmark of cancer. However, whether aneuploidy is a driving cause or a consequence of tumor formation remains controversial. Paradoxically, existing studies based on aneuploid yeast and mouse fibroblasts have shown that aneuploidy is usually detrimental to cellular fitness. Here, we examined the effects of aneuploidy on mouse embryonic stem (ES) cells by generating a series of cell lines that each carries an extra copy of single chromosomes, including trisomy 6, 8, 11, 12, or 15. Most of these aneuploid cell lines had rapid proliferation rates and enhanced colony formation efficiencies. They were less dependent on growth factors for self-renewal and showed a reduced capacity to differentiate in vitro Moreover, trisomic stem cells formed teratomas more efficiently, from which undifferentiated cells can be recovered. Further investigations demonstrated that co-culture of wild-type and aneuploid ES cells or supplementation with extracellular BMP4 rescues the differentiation defects of aneuploid ES cells.
authors: State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.|Department of Medical Genetics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
authors: Meili
authors: M
authors: Zhang
authors: Department of Medical Genetics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
authors: Li
authors: L
authors: Cheng
authors: State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.|Department of Medical Genetics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
authors: Yuyan
authors: Y
authors: Jia
authors: State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.|Department of Medical Genetics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
authors: Guang
authors: G
authors: Liu
authors: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
authors: Cuiping
authors: C
authors: Li
authors: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
authors: Shuhui
authors: S
authors: Song
authors: The Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
authors: Allan
authors: A
authors: Bradley
authors: State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China huangyue@pumc.edu.cn.|Department of Medical Genetics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
authors: Yue
authors: 0000-0003-1800-1282
authors: Y
authors: Huang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D053595:Embryonic Stem Cells / Q000502:physiology*; D008297:Male; D016513:Mice, SCID; D033581:Stem Cell Transplantation; D013724:Teratoma / Q000235:genetics* / Q000473:pathology; D059467:Transcriptome; D014314:Trisomy; D047368:Tumor Burden
keywords: ES cells; aneuploidy; differentiation; extracellular factors; tumorigenesis