title: Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network.
abstract: The water-soluble sulfated xylogalactoarabinans from green seaweed Cladophora falklandica are constituted by a backbone of 4-linked β-l-arabinopyranose units partially sulfated mainly on C3 and also on C2. Besides, partial glycosylation mostly on C2 with single stubs of β-d-xylopyranose, or single stubs of β-d-galactofuranose or short chains comprising (1→5)- and/or (1→6)-linkages, was also found. These compounds showed anticoagulant activity, although much lower than that of heparin. The effect of a purified fraction (F1) on the fibrin network was studied in detail. It modifies the kinetics of fibrin formation, suggesting an impaired polymerization process. Scanning electron microscopy showed a laxer conformation, with larger interstitial pores than the control. Accordingly, this network was lysed more easily. These fibrin properties would reduce the time of permanence of the clot in the blood vessel, inducing a lesser thrombogenic state. One of the possible mechanisms of its anticoagulant effect is direct thrombin inhibition.
authors: Universidad de Buenos Aires, Facultad de Agronomía, Departamento de Biología Aplicada y Alimentos, Cátedra de Química de Biomoléculas, Av. San Martín 4453, C1417DSE Buenos Aires, Argentina.
authors: Paula X
authors: PX
authors: Arata
authors: Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Hemostasia y Trombosis, Ciudad Universitaria - Pabellón 2, C1428EHA Buenos Aires, Argentina.
authors: Irene
authors: I
authors: Quintana
authors: Consejo Nacional de lnvestigaciones Cientificas y Tecnicas-Centro Nacional Patagónico (CENPAT-CONICET), Bvd. Brown 2915, U9120ACD Puerto Madryn, Chubut, Argentina.
authors: María Paula
authors: MP
authors: Raffo
authors: Universidad de Buenos Aires, Facultad de Agronomía, Departamento de Biología Aplicada y Alimentos, Cátedra de Química de Biomoléculas, Av. San Martín 4453, C1417DSE Buenos Aires, Argentina; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Consejo Nacional de lnvestigaciones Cientificas y Tecnicas-Centro de Investigación de Hidratos de Carbono (CIHIDECAR-CONICET), Ciudad Universitaria - Pabellón 2, C1428EHA Buenos Aires, Argentina. Electronic address: ciancia@agro.uba.ar.
authors: Marina
authors: M
authors: Ciancia
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology*; D002236:Carbohydrate Conformation; D000460:Chlorophyta / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005337:Fibrin / Q000378:metabolism*; D005342:Fibrinolysis / Q000187:drug effects; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D011817:Rabbits; D012624:Seaweed / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry; D014994:Xylose / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D000925:Anticoagulants; D011134:Polysaccharides; D013431:Sulfates; C431715:xylopyranose; D005337:Fibrin; D014994:Xylose
keywords: Anticoagulant activity; Cladophora; Fibrin network; Galactofuranose; Green seaweed; Sulfated arabinan