title: Potential efficacy of Lactobacillus casei IBRC_M10711 on expression and activity of insulin degrading enzyme but not insulin degradation.
abstract: Type 2 diabetes (T2D) is a condition with insufficient insulin production or in the setting of insulin resistance with many origins including intestinal microbiota-related molecular mechanism. Insulin-degrading enzyme (IDE) is responsible for insulin breakdown in various tissues and is known as a potential drug target for T2D. Here, we assessed the effects of cell-free supernatant (CFS) and UV-killed Lactobacillus casei IBRC_M10711 on IDE expression, IDE activity, and insulin degradation in Caco-2 cell line. It was found that CFS and UV-killed L. casei IBRC_M10711 led to lower expression of IDE. UV-killed L. casei IBRC_M10711 significantly inhibited IDE activity but CFS did not. Insulin degradation was affected with none of them. In conclusion, L. casei IBRC_M10711 is effective on IDE expression and its activity, but not on insulin degradation. Future studies are recommended to explore the effect of this probiotic on other substrates of IDE.
authors: Department of Medical Biotechnology, School of Advanced Technologies in Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran.|Department of Oncology, Kabul Medical University, Jamal Mena, Kabul, Afghanistan.
authors: Nadia
authors: N
authors: Neyazi
authors: Department of Medical Biotechnology, School of Advanced Technologies in Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran.
authors: Taiebeh
authors: T
authors: Mohammadi Farsani
authors: Department of Medical Biotechnology, School of Advanced Technologies in Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran.
authors: Zahra
authors: Z
authors: Nouri
authors: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.|Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mohammad Hossein
authors: MH
authors: Ghahremani
authors: Department of Medical Biotechnology, School of Advanced Technologies in Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran.|Biosensor Research Center, Endocrinilogy and Metabolism Molecular-Cellular Sciences Institute, EMRI, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mohammad Reza
authors: MR
authors: Khorramizadeh
authors: Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Roksana
authors: R
authors: Tajerian
authors: Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. e_motevaseli@tums.ac.ir.|Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran. e_motevaseli@tums.ac.ir.
authors: Elahe
authors: E
authors: Motevaseli
mesh_terms: D018938:Caco-2 Cells; D049109:Cell Proliferation; D002474:Cell-Free System; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007339:Insulysin / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000378:metabolism* / Q000528:radiation effects; D050296:Microbial Viability / Q000528:radiation effects; D059748:Proteolysis; D014466:Ultraviolet Rays
chemical_list: D007328:Insulin; D007339:Insulysin
keywords: Cell-free supernatant; Insulin degradation; Insulin-degrading enzyme; Lactobacillus casei IBRC_M10711; UV-killed