title: TRPA1 and TRPV1 contribute to iodine antiseptics-associated pain and allergy.
abstract: Iodine antiseptics exhibit superior antimicrobial efficacy and do not cause acquired microbial resistance. However, they are underused in comparison with antibiotics in infection treatments, partly because of their adverse effects such as pain and allergy. The cause of these noxious effects is not fully understood, and no specific molecular targets or mechanisms have been discovered. In this study, we show that iodine antiseptics cause pain and promote allergic contact dermatitis in mouse models, and iodine stimulates a subset of sensory neurons that express TRPA1 and TRPV1 channels. In vivo pharmacological inhibition or genetic ablation of these channels indicates that TRPA1 plays a major role in iodine antiseptics-induced pain and the adjuvant effect of iodine antiseptics on allergic contact dermatitis and that TRPV1 is also involved. We further demonstrate that iodine activates TRPA1 through a redox mechanism but has no direct effects on TRPV1. Our study improves the understanding of the adverse effects of iodine antiseptics and suggests a means to minimize their side effects through local inhibition of TRPA1 and TRPV1 channels.
authors: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, and Ion Channel Research and Drug Development Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, China.
authors: Deyuan
authors: D
authors: Su
authors: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, and Ion Channel Research and Drug Development Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
authors: Hong
authors: H
authors: Zhao
authors: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, and Ion Channel Research and Drug Development Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, China.
authors: Jinsheng
authors: J
authors: Hu
authors: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, and Ion Channel Research and Drug Development Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
authors: Dan
authors: D
authors: Tang
authors: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, and Ion Channel Research and Drug Development Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China Department of Biomedical Engineering, Center for the Investigation of Membrane Excitability Disorders, Cardiac Bioelectricity and Arrhythmia Center, Washington University, St. Louis, MO, USA.
authors: Jianmin
authors: J
authors: Cui
authors: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, and Ion Channel Research and Drug Development Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA.
authors: Ming
authors: M
authors: Zhou
authors: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, and Ion Channel Research and Drug Development Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China Department of Biological Sciences, Columbia University, New York, NY, USA jianyang@mail.kiz.ac.cn jy160@columbia.edu wangshu@mail.kiz.ac.cn.
authors: Jian
authors: 0000-0003-0290-7688
authors: J
authors: Yang
authors: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, and Ion Channel Research and Drug Development Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, China jianyang@mail.kiz.ac.cn jy160@columbia.edu wangshu@mail.kiz.ac.cn.
authors: Shu
authors: S
authors: Wang
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000009:adverse effects*; D002460:Cell Line; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000166:cytology; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis / Q000209:etiology*; D007455:Iodine / Q000009:adverse effects*; D015640:Ion Channel Gating / Q000187:drug effects; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D009154:Mutation; D010146:Pain / Q000175:diagnosis / Q000209:etiology*; D011205:Povidone / Q000009:adverse effects; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000378:metabolism; D000074025:TRPA1 Cation Channel; D050916:TRPV Cation Channels / Q000235:genetics* / Q000378:metabolism; D050051:Transient Receptor Potential Channels / Q000235:genetics* / Q000378:metabolism
chemical_list: D000891:Anti-Infective Agents, Local; D000074025:TRPA1 Cation Channel; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D050051:Transient Receptor Potential Channels; C475685:Trpa1 protein, mouse; C419150:polyvinylpyrrolidone-eicosene copolymer; D007455:Iodine; D011205:Povidone
keywords: TRPA1; TRPV1; allergy; iodine; pain