title: Differentiation of RotaTeq®vaccine strains from wild-type strains using NSP3 gene in reverse transcription polymerase chain reaction assay.
abstract: RotaTeq®is a live attenuated human-bovine reassortant vaccine against rotaviruses that is used worldwide. However, shedding of the virus used in RotaTeq®has been detected in the feces of children following vaccination by the oral route, possibly affecting community immunity. Therefore, a simple and efficient method to discriminate between virulent and RotaTeq®vaccine strains is required. In this study, a novel one-step multiplex reverse-transcription polymerase chain reaction (RT-PCR) assay targeting the NSP3 gene was developed to detect RotaTeq®vaccine strains in fecal samples. RotaTeq®vaccine viruses were successfully distinguished from known wild-type rotavirus genotypes. In addition, the developed assay was able to detect rotaviruses in clinical stool samples obtained from South Korea during the 2011-2013 rotavirus seasons. Of the 1106 stool specimens from children with acute gastroenteritis that were screened, 286 rotaviruses were genotyped. RotaTeq®vaccine strains were identified in 39 samples (13.6%). The novel RT-PCR assay that was developed could be used to detect and discriminate between RotaTeq®vaccine strains that are shed in fecal matter, and to estimate the quantification of virus that has been shed after vaccination.
authors: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea.
authors: Sunyoung
authors: S
authors: Jeong
authors: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea.
authors: Van Thai
authors: VT
authors: Than
authors: Department of Pediatrics, Chung-Ang University College of Medicine, Seoul, South Korea.
authors: Inseok
authors: I
authors: Lim
authors: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea. Electronic address: kimwy@cau.ac.kr.
authors: Wonyong
authors: W
authors: Kim
mesh_terms: D000818:Animals; D002417:Cattle; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D056910:Republic of Korea; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity / Q000502:physiology; D012400:Rotavirus Infections / Q000175:diagnosis / Q000821:virology*; D022243:Rotavirus Vaccines / Q000235:genetics*; D014613:Vaccines, Attenuated / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D017201:Virus Shedding
chemical_list: C082740:NSP3 protein, Rotavirus; C492535:RotaTeq; D022243:Rotavirus Vaccines; D014613:Vaccines, Attenuated; D017361:Viral Nonstructural Proteins
keywords: Genotypes; RotaTeq; Rotavirus; Shedding