title: Autophagy-associated dengue vesicles promote viral transmission avoiding antibody neutralization.
abstract: One of the major defense mechanisms against virus spread in vivo is the blocking of viral infectibility by neutralizing antibodies. We describe here the identification of infectious autophagy-associated dengue vesicles released from infected cells. These vesicles contain viral proteins E, NS1, prM/M, and viral RNA, as well as host lipid droplets and LC3-II, an autophagy marker. The viral RNA can be protected within the autophagic organelles since anti-dengue neutralizing antibodies do not have an effect on the vesicle-mediated transmission that is able to initiate a new round of infection in target cells. Importantly, such infectious vesicles were also detected in a patient serum. Our study suggests that autophagy machinery plays a new role in dengue virus transmission. This discovery explains the inefficiency of neutralizing antibody upon dengue infection as a potential immune evasion mechanism in vivo.
authors: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.|Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors: Yan-Wei
authors: YW
authors: Wu
authors: Institute of Human Genetics, CNRS-UPR1142, Montpellier, France.
authors: Clément
authors: C
authors: Mettling
authors: Institute of Oral Medicine, College of Medicine and Hospital, National Cheng Kung University, Tainan, Taiwan.
authors: Shang-Rung
authors: SR
authors: Wu
authors: Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan.
authors: Chia-Yi
authors: CY
authors: Yu
authors: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.|Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.|Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan.
authors: Guey-Chuen
authors: GC
authors: Perng
authors: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.|Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.|Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan.
authors: Yee-Shin
authors: YS
authors: Lin
authors: Institute of Human Genetics, CNRS-UPR1142, Montpellier, France.
authors: Yea-Lih
authors: YL
authors: Lin
mesh_terms: D000330:Aedes; D000818:Animals; D001343:Autophagy; D002460:Cell Line; D003715:Dengue / Q000378:metabolism / Q000473:pathology / Q000635:transmission; D003716:Dengue Virus / Q000378:metabolism* / Q000472:pathogenicity; D006801:Humans; D012367:RNA, Viral / Q000378:metabolism*; D022142:Secretory Vesicles / Q000378:metabolism / Q000473:pathology / Q000821:virology; D014764:Viral Proteins / Q000378:metabolism*; D057074:Virus Release
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins