title: Extraction optimization, characterization and antioxidant activity of polysaccharide from Gentiana scabra bge.
abstract: In this study, optimization of smashing tissue extraction (STE), preliminary chemical characterization and antioxidant activity in vitro of crude polysaccharides (CPS) from Gentiana scabra bge (G. scabra) were investigated. The optimal extraction conditions were determined as follows: sample particle size of 80mesh, solid/liquid ratio of 1:34, extraction voltage of 157.09V and extraction time of 130.38s. Under these conditions, the extraction yield of CPS had reached 15.03±0.14% (n=3). Chemical composition analysis indicated CPS was mainly composed of mannose, rhamnose, galacturonic acid, glcose, galactose, arabinose and fucose in a molar ratio of 1.00:9.89:51.59:35.37:38.06:99.13:21.34, respectively. The average molecular weight of CPS was estimated to be 3.8×104Da. In addition, the potential antioxidant activity of CPS extracted by STE were demonstrated by DPPH radical scavenging assay, superoxide anion radical scavenging assay, hydroxyl radical scavenging assay and ferric reducing power assay. Overall, this study provided an effective extraction technique for G. scabra polysaccharides which would be explored as a promising natural antioxidant agent applied in functional foods or medicines.
authors: School of Chemistry & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin, 132022, China.
authors: Zhenyu
authors: Z
authors: Cheng
authors: School of Chemistry & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin, 132022, China.
authors: Yuewei
authors: Y
authors: Zhang
authors: Laboratory of Test Center, Jilin Agricultural Science and Technology College, Jilin, 132101 China, China.
authors: Haiyan
authors: H
authors: Song
authors: School of Chemistry & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin, 132022, China.
authors: Hongli
authors: H
authors: Zhou
authors: School of Chemistry & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin, 132022, China. Electronic address: 844503608@qq.com.
authors: Fangli
authors: F
authors: Zhong
authors: College of Chemistry & Chemical Engineering, Longdong University, Qingyang, 745000, China.
authors: Haobin
authors: H
authors: Hu
authors: School of Chemistry & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin, 132022, China.
authors: Yu
authors: Y
authors: Feng
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification*; D030012:Gentiana / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides
keywords: Antioxidant activity; Characterization; Smashing tissue extraction