title: ε-Caprolactone-Modified Polyethylenimine as Efficient Nanocarriers for siRNA Delivery in Vivo.
abstract: RNA interference (RNAi) therapy is a promising treatment for various diseases. However, its application is still restricted by the lack of efficient and safe delivery systems. A novel siRNA delivery vehicle based on ε-caprolactone-modified polyethylenimine (PEI-CL) is presented here. The PEI-CL macromolecules with different grafting degrees were synthesized via a simple ring-opening reaction. This macromolecule strongly protects the siRNA from degradation in serum and promotes the cellular uptake and endosomal escape detected via chemical exchange saturation transfer magnetic resonance analysis and fluorescence imaging. The in vivo measurement was performed with HCT-116 colon tumor xenograft that stably expressed luciferase. The data showed that the PEI-CL/siRNA nanocomplexes elicited strong RNAi response. More interestingly, enhanced gene transfection efficiency was achieved by simultaneous cotransfection with siRNA and DNA plasmid via this novel nanosystem. Overall, our study suggests the PEI-CL macromolecule with great promise for siRNA delivery.
authors: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055, P.R. China.|State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University , Xiamen 361102, P.R. China.|Department of Chemical and Biomolecular Engineering and Institute for NanoBio Technology, The Johns Hopkins University , 3400 N Charles Street, Baltimore, Maryland 21218, United States.
authors: Lisi
authors: L
authors: Xie
authors: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055, P.R. China.
authors: Yan
authors: Y
authors: Tan
authors: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055, P.R. China.
authors: Zhiyong
authors: Z
authors: Wang
authors: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055, P.R. China.|School of Chemical Engineering and Light Industry, Guangdong University of Technology , Guangzhou, Guangdong 510006, P.R. China.
authors: Hong
authors: H
authors: Liu
authors: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055, P.R. China.
authors: Na
authors: N
authors: Zhang
authors: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055, P.R. China.
authors: Chao
authors: C
authors: Zou
authors: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055, P.R. China.
authors: Xin
authors: X
authors: Liu
authors: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University , Xiamen 361102, P.R. China.
authors: Gang
authors: G
authors: Liu
authors: National Engineering Research Center for Biomaterials, Sichuan University , Chengdu 610064, P.R. China.
authors: Jian
authors: J
authors: Lu
authors: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055, P.R. China.
authors: Hairong
authors: H
authors: Zheng
mesh_terms: D002208:Caproates / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry*; D011094:Polyethyleneimine; D034741:RNA, Small Interfering
chemical_list: D002208:Caproates; D007783:Lactones; D034741:RNA, Small Interfering; C121056:caprolactone; D011094:Polyethyleneimine
keywords: RNA interference therapy; nanoparticle; polyethylenimine; self-assembly; small interfering RNA