title: Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer.
abstract: OBJECTIVE
  Long non-coding RNAs can regulate tumorigenesis of various cancers. Dys-regulation of lncRNA-AFAP1-AS1 has not been studied in colorectal carcinoma (CRC). This study was to examine the function involvement of AFAP1-AS1 in tumor growth and metastasis of CRC.
  
  
  METHODS
  Relative expression of AFAP1-AS1 in CRC tissues and CRC cells lines was determined using quantitative real-time PCR (qRT-PCR). Functional involvement of AFAP1-AS1 in tumor proliferation and metastasis was evaluated in AFAP1-AS1-specific siRNA-treated CRC cells and in CRC cell xenograft. Expression of epithelial-mesenchymal transition (EMT)-related gene expression was determined using western blot.
  
  
  RESULTS
  Relative expression of AFAP1-AS1 was significantly elevated in CRC tissues and CRC HCT116 and SW480 cell lines. AFAP1-AS1 knock-down suppressed SW480 cell proliferation, colony formation, migration and invasion. Also AFAP1-AS1 knock-down inhibited tumor metastasis-associated genes expression in terms of EMT. This carcinostatic action by AFAP1-AS1 knock-down was further confirmed by suppression of tumor formation and hepatic metastasis of CRC cells in nude mice.
  
  
  CONCLUSIONS
  lncRNA-AFAP1-AS1 knock-down exhibits antitumor effect on colorectal carcinoma in respects of suppression of cell proliferation and metastasis of cancer cells.
authors: General surgery, Chao-Yang Hospital of Capital Medical University, 8 Gongtinan Rd, Beijing, 10001, People's Republic of China.|Anorectal surgery, Beihua University, Jilin, 132001, People's Republic of China.
authors: Xu
authors: X
authors: Han
authors: Department of Neurology, Beihua University, Jilin, 132001, People's Republic of China.
authors: Lingling
authors: L
authors: Wang
authors: Anorectal surgery, Beihua University, Jilin, 132001, People's Republic of China.
authors: Yu
authors: Y
authors: Ning
authors: Anorectal surgery, Beihua University, Jilin, 132001, People's Republic of China.
authors: Shuang
authors: S
authors: Li
authors: General surgery, Chao-Yang Hospital of Capital Medical University, 8 Gongtinan Rd, Beijing, 10001, People's Republic of China. zhjun_wang@163.com.
authors: Zhenjun
authors: Z
authors: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000556:secondary*; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D045325:HCT116 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000556:secondary*; D008297:Male; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D062085:RNA, Long Noncoding / Q000032:analysis / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: C000605367:AFAP1-AS1 long noncoding RNA, human; D062085:RNA, Long Noncoding
keywords: AFAP1-AS1; Colorectal cancer; Epithelial–mesenchymal transition (EMT); Tumor metastasis