title: Long noncoding RNA #32 contributes to antiviral responses by controlling interferon-stimulated gene expression.
abstract: Despite the breadth of knowledge that exists regarding the function of long noncoding RNAs (lncRNAs) in biological phenomena, the role of lncRNAs in host antiviral responses is poorly understood. Here, we report that lncRNA#32 is associated with type I IFN signaling. The silencing of lncRNA#32 dramatically reduced the level of IFN-stimulated gene (ISG) expression, resulting in sensitivity to encephalomyocarditis virus (EMCV) infection. In contrast, the ectopic expression of lncRNA#32 significantly suppressed EMCV replication, suggesting that lncRNA#32 positively regulates the host antiviral response. We further demonstrated the suppressive function of lncRNA#32 in hepatitis B virus and hepatitis C virus infection. lncRNA#32 bound to activating transcription factor 2 (ATF2) and regulated ISG expression. Our results reveal a role for lncRNA#32 in host antiviral responses.
authors: Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Ichikawa, Chiba 272-8516, Japan lbnishitsuji@hospk.ncgm.go.jp.
authors: Hironori
authors: H
authors: Nishitsuji
authors: Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Ichikawa, Chiba 272-8516, Japan.
authors: Saneyuki
authors: S
authors: Ujino
authors: Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Ichikawa, Chiba 272-8516, Japan.
authors: Sachiyo
authors: S
authors: Yoshio
authors: Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Ichikawa, Chiba 272-8516, Japan.
authors: Masaya
authors: M
authors: Sugiyama
authors: Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Ichikawa, Chiba 272-8516, Japan.
authors: Masashi
authors: M
authors: Mizokami
authors: Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Ichikawa, Chiba 272-8516, Japan.
authors: Tatsuya
authors: T
authors: Kanto
authors: Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Ichikawa, Chiba 272-8516, Japan.
authors: Kunitada
authors: K
authors: Shimotohno
mesh_terms: D051698:Activating Transcription Factor 2 / Q000235:genetics* / Q000378:metabolism; D018188:Cardiovirus Infections / Q000235:genetics / Q000821:virology; D045744:Cell Line, Tumor; D004686:Encephalomyocarditis virus / Q000235:genetics / Q000472:pathogenicity; D005786:Gene Expression Regulation; D020868:Gene Silencing; D016174:Hepacivirus / Q000235:genetics / Q000472:pathogenicity; D006526:Hepatitis C / Q000235:genetics / Q000821:virology; D022781:Hepatocytes / Q000378:metabolism / Q000821:virology; D054884:Host-Pathogen Interactions / Q000235:genetics*; D006801:Humans; D007370:Interferon Type I / Q000235:genetics* / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
chemical_list: C497105:ATF2 protein, human; D051698:Activating Transcription Factor 2; D007370:Interferon Type I; D062085:RNA, Long Noncoding
keywords: ISG; innate immunity; interferon; lncRNA