title: Silver nanoparticles ensemble with Zn(II) complex of terpyridine as a highly sensitive colorimetric assay for the detection of Arginine.
abstract: Arginine play numerous roles in several biological processes, and also act as a precursor for many biomolecules. Arginine derived biomolecules were found to involve in many of the events that lead to diseases and therefore selective detection of Arg in biological fluids has an enormous impact on disease diagnosis and in the drug development. Although number of methods were developed for the selective detection of Arg, the colorimetric method has an advantage over these methods due to their operational simplicity, high selectivity, and speediness. Herein, silver nanoparticles ensemble with Zn(II) complex of α-liopoic acid conjugated terpyridine (ZnLATP-AgNPs) were developed for the selective colorimetric detection of Arg. The nanoparticle ensemble exhibited selectivity towards Arg by showing distinguishable colour change from yellow to orange, among all the other naturally occurring amino acids studied. The new ZnLATP-AgNPs assay allows detection of Arg down to 200±15nM, provides an easy and sensitive method to detect Arg visually. The current approach was further validated by the quantification of Arg in supplement tablets.
authors: Analytical Division and Centralized Instrument Facility, CSIR-Central Salt & Marine Chemicals Research Institute, Bhavnagar 364002, India.
authors: Krishna
authors: K
authors: Velugula
authors: Analytical Division and Centralized Instrument Facility, CSIR-Central Salt & Marine Chemicals Research Institute, Bhavnagar 364002, India. Electronic address: jpchinta@csmcri.org.
authors: Jugun Prakash
authors: JP
authors: Chinta
mesh_terms: D001120:Arginine / Q000032:analysis*; D003124:Colorimetry / Q000379:methods*; D056831:Coordination Complexes / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013607:Tablets; D015032:Zinc / Q000737:chemistry*
chemical_list: C512247:2,3',6',3''-terpyridine; D056831:Coordination Complexes; D011725:Pyridines; D013607:Tablets; D012834:Silver; D001120:Arginine; D015032:Zinc
keywords: Arginine; Colorimetric sensing; Silver nanoparticles; Terpyridine; Zn(II) complex