title: Opposing Control by Transcription Factors MYB61 and MYB3 Increases Freezing Tolerance by Relieving C-Repeat Binding Factor Suppression.
abstract: Cold acclimation is an important process by which plants respond to low temperature and enhance their winter hardiness. C-REPEAT BINDING FACTOR1 (CBF1), CBF2, and CBF3 genes were shown previously to participate in cold acclimation in Medicago truncatula In addition, MtCBF4 is transcriptionally induced by salt, drought, and cold stresses. We show here that MtCBF4, shown previously to enhance drought and salt tolerance, also positively regulates cold acclimation and freezing tolerance. To identify molecular factors acting upstream and downstream of the MtCBF4 transcription factor (TF) in cold responses, we first identified genes that are differentially regulated upon MtCBF4 overexpression using RNAseq Digital Gene Expression Profiling. Among these, we showed that MtCBF4 directly activates the transcription of the COLD ACCLIMATION SPECIFIC15 (MtCAS15) gene. To gain insights into how MtCBF4 is transcriptionally regulated in response to cold, an R2R3-MYB TF, MtMYB3, was identified based on a yeast one-hybrid screen as binding directly to MYB cis-elements in the MtCBF4 promoter, leading to the inhibition of MtCBF4 expression. In addition, another MYB TF, MtMYB61, identified as an interactor of MtMYB3, can relieve the inhibitory effect of MtMYB3 on MtCBF4 transcription. This study, therefore, supports a model describing how MtCBF4 is regulated by antagonistic MtMYB3/MtMYB61 TFs, leading to the up-regulation of downstream targets such as MtCAS15 acting in cold acclimation in M. truncatula.
authors: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.).
authors: Zhenqian
authors: 0000-0003-0210-9408
authors: Z
authors: Zhang
authors: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.).
authors: Xiaona
authors: 0000-0002-6839-3272
authors: X
authors: Hu
authors: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.).
authors: Yunqin
authors: Y
authors: Zhang
authors: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.).
authors: Zhenyan
authors: Z
authors: Miao
authors: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.).
authors: Can
authors: C
authors: Xie
authors: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.).
authors: Xiangzhao
authors: X
authors: Meng
authors: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.).
authors: Jie
authors: J
authors: Deng
authors: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.).
authors: Jiangqi
authors: 0000-0001-5113-7750
authors: J
authors: Wen
authors: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.).
authors: Kirankumar S
authors: KS
authors: Mysore
authors: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.).
authors: Florian
authors: F
authors: Frugier
authors: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.).
authors: Tao
authors: T
authors: Wang
authors: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Z.Z., X.H., Y.Z., Z.M. C.X., X.M., J.D., T.W., J.D.);Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401 (J.W., K.S.M.); andInstitute of Plant Sciences Paris-Saclay, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Paris-Sud, Universite Paris-Diderot, Universite d'Evry, Universite Paris-Saclay, Gif-sur-Yvette 91190, France (F.F.) dongjl@cau.edu.cn.
authors: Jiangli
authors: 0000-0002-5098-8681
authors: J
authors: Dong
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D001483:Base Sequence; D047369:Chromatin Immunoprecipitation; D005615:Freezing; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D046913:Medicago truncatula / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors