title: Truncation Derivatives of the S-Layer Protein of Sporosarcina ureae ATCC 13881 (SslA): Towards Elucidation of the Protein Domain Responsible for Self-Assembly.
abstract: The cell surface of Sporosarcina ureae ATCC 13881 is covered by an S-layer (SslA) consisting of identical protein subunits that assemble into lattices exhibiting square symmetry. In this work the self-assembly properties of the recombinant SslA were characterised with an emphasis on the identification of protein regions responsible for self-assembly. To this end, recombinant mature SslA (aa 31-1097) and three SslA truncation derivatives (one N-terminal, one C-terminal and one CN-terminal) were produced in a heterologous expression system, isolated, purified and their properties analysed by in vitro recrystallisation experiments on a functionalised silicon wafer. As a result, recombinant mature SslA self-assembled into crystalline monolayers with lattices resembling the one of the wild-type SslA. The study identifies the central protein domain consisting of amino acids 341-925 self-sufficient for self-assembly. Neither the first 341 amino acids nor the last 172 amino acids of the protein sequence are required to self-assemble into lattices.
authors: Electronics Packaging Laboratory, Department of Electrical Engineering and Information Technology, Technische Universität Dresden, 01069 Dresden, Germany. varga@avt.et.tu-dresden.de.
authors: Melinda
authors: M
authors: Varga
mesh_terms: D000595:Amino Acid Sequence; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics; D000072417:Protein Domains; D011994:Recombinant Proteins; D017384:Sequence Deletion; D058644:Sporosarcina / Q000737:chemistry* / Q000235:genetics
chemical_list: D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; C055764:S-layer proteins
keywords: Sporosarcina ureae ATCC 13881; mutagenesis; self-assembly; surface layer protein