title: A robust ambient temperature collection and stabilization strategy: Enabling worldwide functional studies of the human microbiome.
abstract: As reports on possible associations between microbes and the host increase in number, more meaningful interpretations of this information require an ability to compare data sets across studies. This is dependent upon standardization of workflows to ensure comparability both within and between studies. Here we propose the standard use of an alternate collection and stabilization method that would facilitate such comparisons. The DNA Genotek OMNIgene∙Gut Stool Microbiome Kit was compared to the currently accepted community standard of freezing to store human stool samples prior to whole genome sequencing (WGS) for microbiome studies. This stabilization and collection device allows for ambient temperature storage, automation, and ease of shipping/transfer of samples. The device permitted the same data reproducibility as with frozen samples, and yielded higher recovery of nucleic acids. Collection and stabilization of stool microbiome samples with the DNA Genotek collection device, combined with our extraction and WGS, provides a robust, reproducible workflow that enables standardized global collection, storage, and analysis of stool for microbiome studies.
authors: Human Longevity, Inc., San Diego, CA 92121, USA.
authors: Ericka L
authors: EL
authors: Anderson
authors: Human Longevity, Inc., San Diego, CA 92121, USA.|Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA.
authors: Weizhong
authors: W
authors: Li
authors: Human Longevity, Inc., San Diego, CA 92121, USA.
authors: Niels
authors: N
authors: Klitgord
authors: Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA.
authors: Sarah K
authors: SK
authors: Highlander
authors: Human Longevity, Inc., San Diego, CA 92121, USA.
authors: Mark
authors: M
authors: Dayrit
authors: Human Longevity, Inc., San Diego, CA 92121, USA.
authors: Victor
authors: V
authors: Seguritan
authors: Human Longevity, Inc., San Diego, CA 92121, USA.|Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA.
authors: Shibu
authors: S
authors: Yooseph
authors: Human Longevity, Inc., San Diego, CA 92121, USA.
authors: William
authors: W
authors: Biggs
authors: Human Longevity, Inc., San Diego, CA 92121, USA.|Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA.
authors: J Craig
authors: JC
authors: Venter
authors: Human Longevity, Inc., San Diego, CA 92121, USA.|Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA.
authors: Karen E
authors: KE
authors: Nelson
authors: Human Longevity, Inc., San Diego, CA 92121, USA.
authors: Marcus B
authors: MB
authors: Jones
mesh_terms: D000465:Algorithms; D015331:Cohort Studies; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D005243:Feces; D005615:Freezing; D006801:Humans; D016014:Linear Models; D064307:Microbiota; D015233:Models, Statistical; D009696:Nucleic Acids / Q000737:chemistry; D016366:Open Reading Frames; D011786:Quality Control; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D000073336:Whole Genome Sequencing
chemical_list: D004269:DNA, Bacterial; D009696:Nucleic Acids