title: Hepatoprotective effect of Quercetin supplementation against Acrylamide-induced DNA damage in wistar rats.
abstract: BACKGROUND
  Quercetin (QR), is a polyphenolic flavonoid compound which is found in large amounts in certain foods, and protects against oxidative stress. The current study was conducted to determine whether Quercetin can possibly exert hepatoprotective and antioxidant activity against acrylamide (ACR) induced toxicity in rats.
  
  
  METHODS
  Four groups of Wistar rats consisting of six rats each: (i) control group; (ii) ACR treated group (50 mg/kg bw); (iii) QR group: rats were treated with QR (10 mg/kg bw); (iv) QR (10 mg/kg bw) was given i.p. for 5 days followed by ACR (50 mg/kg bw) on 5th day (single dose).
  
  
  RESULTS
  ACR caused an elevation in 8-OH guanosine level and a reduction in Glutahione S-transferase (GST) activity. Administration of QR significantly protected liver tissue against hepatotoxic effect of acrylamide from amelioration of the marker enzyme (p < 0.05) and DNA damage (p < 0.01) as evident by comet assay and, besides some indices of histopathological alterations.
  
  
  CONCLUSIONS
  It is concluded that QR could protect the liver against DNA damage induced by ACR probably is thus capable of ameliorating ACR-induced changes in the rat livers.
authors: Clinical Laboratory Sciences, College of Applied Medical Science, King Saud University, Riyadh, Saudi Arabia. sansar@ksu.edu.sa.
authors: Sabah
authors: S
authors: Ansar
authors: Biochemistry Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors: Nikhat Jamal
authors: NJ
authors: Siddiqi
authors: Biochemistry Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors: Seema
authors: S
authors: Zargar
authors: Department of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
authors: Majid Ahmad
authors: MA
authors: Ganaie
authors: Clinical Laboratory Sciences, College of Applied Medical Science, King Saud University, Riyadh, Saudi Arabia.
authors: Manal
authors: M
authors: Abudawood
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D022781:Hepatocytes / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D020106:Acrylamide; D011794:Quercetin
keywords: Antioxidants; DNA damage; Oxidative stress; Quercetin