title: Lipid peroxidation as risk factor for endothelial dysfunction in antiphospholipid syndrome patients.
abstract: The aim of this study was to evaluate oxidative stress markers and it relations to endothelial damage as risk factor for thrombosis in patients with primary (PAPS) and secondary (SAPS) antiphospholipid syndrome (APS) in correlation to traditional risk factors. Flow-mediated (FMD) and nitroglycerine (NMD)-induced dilation of the brachial artery were studied in 140 APS patients (90 PAPS, 50 SAPS) and 40 controls matched by age, sex, and conventional risk factors for atherosclerosis. Markers of oxidative stress, lipid hydroperoxydes (LOOH), advanced oxidation protein products (AOPP), total sulfhydryl groups (tSHG), and paraoxonase 1 activity (PON1) were determined by spectrophotometric method. Oxidative stress dominates in APS patients. LOOH and AOPP correlate to lipid fractions (p < 0.05), unlike PON1, tSHG that correlated to antiphospholipid antibody positivity (p < 0.05). FMD was lower in APS patients comparing to controls (p < 0.001). Cholesterol is independent variable for FMD impairment in control group (p = 0.011); LOOH in PAPS (p = 0.004); LOOH, aCL, and triglycerides in SAPS patients (p = 0.009, p = 0.049, and p = 0.012, respectively). Combined predictive of aCL and LOOH is better for FMD impairment than LOOH alone in both PAPS and SAPS patients (AUC 0.727, p = 0.001, 95 % CI 0.616-0.837 and AUC 0.824, p˂0.001, 95 % CI 0.690-0.957, respectively). Lipid peroxidation is independent predictor for endothelial dysfunction in APS patients. We demonstrated synergistic effect of aCL and LOOH as risk for endothelial impairment in both PAPS and SAPS patients.
authors: University Clinical Center "Bezanijska kosa", Bezanijska kosa bb, Belgrade, 11070, Serbia. nacastanisavljevic@gmail.com.
authors: Natasa
authors: N
authors: Stanisavljevic
authors: University Clinical Center "Bezanijska kosa", Bezanijska kosa bb, Belgrade, 11070, Serbia.
authors: L
authors: L
authors: Stojanovich
authors: University Clinical Center "Bezanijska kosa", Bezanijska kosa bb, Belgrade, 11070, Serbia.|Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors: D
authors: D
authors: Marisavljevic
authors: University Clinical Center "Bezanijska kosa", Bezanijska kosa bb, Belgrade, 11070, Serbia.|Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors: A
authors: A
authors: Djokovic
authors: Clinical Center of Serbia, Faculty of Pharmacy Belgrade, University of Belgrade, Belgrade, Serbia.
authors: V
authors: V
authors: Dopsaj
authors: Institute of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
authors: J
authors: J
authors: Kotur-Stevuljevic
authors: Rakovica Community Health Center, Belgrade, Serbia.
authors: J
authors: J
authors: Martinovic
authors: University Clinical Center "Bezanijska kosa", Bezanijska kosa bb, Belgrade, 11070, Serbia.
authors: L
authors: L
authors: Memon
authors: University Clinical Center "Bezanijska kosa", Bezanijska kosa bb, Belgrade, 11070, Serbia.
authors: S
authors: S
authors: Radovanovic
authors: University Clinical Center "Bezanijska kosa", Bezanijska kosa bb, Belgrade, 11070, Serbia.
authors: B
authors: B
authors: Todic
authors: University Clinical Center "Bezanijska kosa", Bezanijska kosa bb, Belgrade, 11070, Serbia.
authors: D
authors: D
authors: Lisulov
mesh_terms: D000328:Adult; D016736:Antiphospholipid Syndrome / Q000150:complications / Q000378:metabolism / Q000503:physiopathology*; D043303:Aryldialkylphosphatase / Q000378:metabolism; D015415:Biomarkers; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D012307:Risk Factors; D013927:Thrombosis / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology
chemical_list: D015415:Biomarkers; D043303:Aryldialkylphosphatase
keywords: Antiphospholipid syndrome; Endothelial dysfunction; Oxidative stress; Thrombosis