title: A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells.
abstract: Intracellular viscosity is a fundamental physical parameter that indicates the functioning of cells. In this work, we developed a simple boron-dipyrromethene (BODIPY)-based probe, BTV, for cellular mitochondria viscosity imaging by coupling a simple BODIPY rotor with a mitochondria-targeting unit. The BTV exhibited a significant fluorescence intensity enhancement of more than 100-fold as the solvent viscosity increased. Also, the probe showed a direct linear relationship between the fluorescence lifetime and the media viscosity, which makes it possible to trace the change of the medium viscosity. Furthermore, it was demonstrated that BTV could achieve practical applicability in the monitoring of mitochondrial viscosity changes in live cells through fluorescence lifetime imaging microscopy (FLIM).
authors: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore 138667, Singapore. sudongdong1984@gmail.com.
authors: Dongdong
authors: D
authors: Su
authors: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore 138667, Singapore. chai.teoh@gmail.com.
authors: Chai Lean
authors: CL
authors: Teoh
authors: Department of Chemistry, National University of Singapore, Singapore 117543, Singapore. chmgn@nus.edu.sg.
authors: Nengyue
authors: N
authors: Gao
authors: Department of Chemistry, National University of Singapore, Singapore 117543, Singapore. chmxqh@nus.edu.sg.
authors: Qing-Hua
authors: QH
authors: Xu
authors: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore 138667, Singapore. chmcyt@nus.edu.sg.|Department of Chemistry, National University of Singapore, Singapore 117543, Singapore. chmcyt@nus.edu.sg.
authors: Young-Tae
authors: YT
authors: Chang
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D002470:Cell Survival; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D015335:Molecular Probes / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014783:Viscosity
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D015335:Molecular Probes; D012997:Solvents
keywords: BODIPY; FLIM; cell imaging; fluorescent probes; sensors; viscosity