title: Factors of detection of Bacillus cereus strains in eye cream.
abstract: Bacillus cereus has been associated with clinical infections and is also the cause of post-traumatic endophthalmitis as well as endogenous eye infections, which can result in blindness. Cosmetics, although preserved, can be contaminated during manufacture or use and thus cause serious health issues.
  
  
  OBJECTIVE
  We investigated the detection factors of Bacillus in eye cream preserved with parabens, including the non-ionic surfactants used as neutralizers such as Tween 80, a blend of Tween 60 and Span 80, Tween 20 and selective media.
  
  
  METHODS
  Eye-cream samples were first mixed with neutralizers and individually inoculated with B. cereus strains, B. mycoides, B. subtilis or B. thuringiensis at a final concentration of 5 log CFU per g. The inoculated samples with and without neutralizers were analysed after 30 min and during 84-day storage at room temperature. Presumptive colonies of Bacillus were enumerated on the varieties of Bacillus agar by spiral-plating techniques and most probable number (MPN) method.
  
  
  RESULTS
  The recovery counts of all Bacillus strains were between 4.10 and 4.58 log CFU per g in samples with Tween 80 and from 3.62 to 4.53 CFU per g in samples with TS after 30 min. Tween 20 was the least effective neutralizer. The challenged organisms, in samples without neutralizer, B. subtilis ATCC 15563 and B. cereus 4227A were detected at 1.83 and 1.49 log CFU per g after 30 min, respectively.
  
  
  CONCLUSIONS
  This study showed that Tween 80 was the best neutralizer for reducing the antimicrobial effect of parabens. BACARA®and R&F plating media showed typical reaction of Bacillus cereus strains.
authors: Oak Ridge Institute for Science and Education, 1299 Bethel Valley Rd Oak Ridge, TN 37830, USA.|U.S. Food and Drug Administration, Office of Regulatory Science, 5001 Campus Dr, College Park, MD 20740, USA.
authors: N
authors: N
authors: Yossa
authors: Oak Ridge Institute for Science and Education, 1299 Bethel Valley Rd Oak Ridge, TN 37830, USA.
authors: G
authors: G
authors: Arce
authors: U.S. Food and Drug Administration, Office of Cosmetics and Colors, 5001 Campus Dr, College Park, MD 20740, USA.
authors: M-C J
authors: MJ
authors: Huang
authors: U.S. Food and Drug Administration, Office of Analytics and Outreach, 5001 Campus Dr, College Park, MD 20740, USA.
authors: L
authors: L
authors: Yin
authors: U.S. Food and Drug Administration, Office of Regulatory Science, 5001 Campus Dr, College Park, MD 20740, USA.
authors: E
authors: E
authors: Brown
authors: U.S. Food and Drug Administration, Office of Regulatory Science, 5001 Campus Dr, College Park, MD 20740, USA.
authors: T
authors: T
authors: Hammack
mesh_terms: D001409:Bacillus cereus / Q000145:classification / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D003358:Cosmetics; D003470:Culture Media; D006801:Humans; D008826:Microbial Sensitivity Tests; D010226:Parabens / Q000494:pharmacology; D013045:Species Specificity
chemical_list: D003358:Cosmetics; D003470:Culture Media; D010226:Parabens
keywords: 
  B. cereus
  ; eye cream; neutralizers; parabens; selective media