title: MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer.
abstract: BACKGROUND
  To investigate the expression of MMP-14 and CD44 as well as epithelial-to-mesenchymal transition(EMT)-like changes in ovarian cancer and to determine correlations with clinical outcome.
  
  
  METHODS
  In 97 patients with ovarian cancer, MMP-14 and CD44 expression as determined by immunohistochemistry was investigated in relation to EMT-like changes. To determine this, immunohistochemical staining of E-cadherin and vimentin was performed.
  
  
  RESULTS
  Patients with expression of both MMP-14 and CD44 in their tumors had a poor prognosis despite complete debulking. Serous histology in advanced-stage tumors (FIGO IIB-IV) correlated with CD44 (rho .286, p < 0.01). Also, CD44 correlated with percentage vimentin expression (rho .217, p < 0.05). In logistic regression analysis with complete debulking as the outcome parameter, CD44 expression was found to be significant (OR 3,571 (95 % Confidence Interval 1,112-11,468) p = 0.032), though this was not the case for MMP-14 and EMT parameters.
  
  
  CONCLUSIONS
  The subgroup of patients with double expression of MMP-14 and CD44 had a poor prognosis despite complete debulking. Serous subtype in advanced-stage patients and CD44 expression were found to be correlated with vimentin expression, and CD44 expression was found to be significantly correlated with complete debulking. However, a significant correlation between EMT and clinical parameters was not found.
authors: Department of Obstetrics and Gynaecology, Elisabeth-Tweesteden Hospital, PO Box 90151, 5000, LC, Tilburg, The Netherlands. c.vos@etz.nl.
authors: Maria Caroline
authors: http://orcid.org/0000-0002-1612-0395
authors: MC
authors: Vos
authors: Department of Obstetrics and Gynaecology, Elisabeth-Tweesteden Hospital, PO Box 90151, 5000, LC, Tilburg, The Netherlands.|Department of Pathology, Elisabeth-Tweesteden Hospital, PO Box 90151, 5000, LC, Tilburg, The Netherlands.
authors: Eva
authors: E
authors: Hollemans
authors: Netherlands Comprehensive Cancer Organisation, Utrecht, The Netherlands.|CoRPS, Tilburg University, Tilburg, The Netherlands.
authors: Nicole
authors: N
authors: Ezendam
authors: Department of Obstetrics and Gynecology, Amphia Hospital, Breda, The Netherlands.
authors: Harry
authors: H
authors: Feijen
authors: Department of Obstetrics and Gynecology, Elisabeth Tweesteden Hospital, Tilburg, The Netherlands.|Present address: Department of Obstetrics and Gynaecology, Catharina Hospital, Eindhoven, The Netherlands.
authors: Dorry
authors: D
authors: Boll
authors: Department of Obstetrics and Gynaecology, Jeroen Bosch Hospital, 's-Hertogenbosch, The Netherlands.
authors: Brenda
authors: B
authors: Pijlman
authors: Present address: Department of Obstetrics and Gynaecology, Catharina Hospital, Eindhoven, The Netherlands.
authors: Hans
authors: H
authors: van der Putten
authors: PAMM, Laboratory for Pathology, Eindhoven, The Netherlands.
authors: Paul
authors: P
authors: Klinkhamer
authors: Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Centre, PO Box 9101, 6500, HB, Nijmegen, The Netherlands.
authors: Toin H
authors: TH
authors: van Kuppevelt
authors: Department of Pathology, Elisabeth-Tweesteden Hospital, PO Box 90151, 5000, LC, Tilburg, The Netherlands.
authors: Anneke A M
authors: AA
authors: van der Wurff
authors: Department of Obstetrics and Gynecology, Radboud University Medical Centre, PO Box 9101, 6500, HB, Nijmegen, The Netherlands.
authors: Leon F A G
authors: LF
authors: Massuger
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D015820:Cadherins / Q000096:biosynthesis; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis* / Q000235:genetics; D053511:Matrix Metalloproteinase 14 / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D014746:Vimentin / Q000096:biosynthesis
chemical_list: D014408:Biomarkers, Tumor; C497483:CD44 protein, human; D015820:Cadherins; D018960:Hyaluronan Receptors; D014746:Vimentin; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14
keywords: CD44; Epithelial-to-mesenchymal transition; MMP-14; Ovarian cancer