title: The host protein CLUH participates in the subnuclear transport of influenza virus ribonucleoprotein complexes.
abstract: The nucleus is highly compartmentalized yet dynamic. Subnuclear functions are regulated by controlling the subnuclear localization of the nuclear proteins. Influenza viral ribonucleoprotein (vRNP) is replicated in the nucleus and then exported to the cytoplasm. However, the precise subnuclear localization and transport of vRNPs remain unclear. Here, we show that CLUH, a host protein whose cellular function is not well established, plays a key role in the subnuclear transport of vRNP. Viral PB2 and M1 induced CLUH translocation to the nucleoplasm and SC35-positive speckles, respectively, even though CLUH is usually cytoplasmic. CLUH depletion inhibited the translocation of M1 to SC35-positive speckles, but did not interfere with PB2 localization to the nucleoplasm and disrupted the subnuclear transport of vRNP, abolishing vRNP nuclear export without affecting viral RNA or protein expression. Our findings suggest that CLUH plays a role in the subnuclear transport of progeny vRNP.
authors: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.|Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan.
authors: Tomomi
authors: T
authors: Ando
authors: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
authors: Seiya
authors: S
authors: Yamayoshi
authors: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.|Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan.
authors: Yuriko
authors: Y
authors: Tomita
authors: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.|Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan.
authors: Shinji
authors: S
authors: Watanabe
authors: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.|Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan.
authors: Tokiko
authors: T
authors: Watanabe
authors: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.|Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan.|Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53711, USA.
authors: Yoshihiro
authors: Y
authors: Kawaoka
mesh_terms: D021581:Active Transport, Cell Nucleus; D005136:Eye Proteins / Q000378:metabolism*; D057809:HEK293 Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D009975:Orthomyxoviridae / Q000502:physiology*; D012261:Ribonucleoproteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: C405933:CLUL1 protein, human; D005136:Eye Proteins; D012261:Ribonucleoproteins; D014764:Viral Proteins