title: HIV-1 clade C escapes broadly neutralizing autologous antibodies with N332 glycan specificity by distinct mechanisms.
abstract: The glycan supersite centered on N332 in the V3 base of the HIV-1 envelope (Env) is a target for broadly neutralizing antibodies (bnAbs) such as PGT121 and PGT128. In this study, we examined the basis of resistance of HIV-1 clade C Envs obtained from broadly cross neutralizing (BCN) plasma of an Indian donor with N332 specificity. Pseudotyped viruses expressing autologous envs were found to be resistant to autologous BCN plasma as well as to PGT121 and PGT128 mAbs despite the majority of Envs containing an intact N332 residue. While resistance of one of the Envs to neutralization by autologous plasma antibodies with shorter V1 loop length was found to be correlated with a N332S mutation, resistance to neutralization of rest of the Envs was found to be associated with longer V1 loop length and acquisition of protective N-glycans. In summary, we show evidence of escape of circulating HIV-1 clade C in an individual from autologous BCN antibodies by three distinct mechanisms.
authors: HIV Vaccine Translational Research Laboratory, NCR Biotech Science Cluster, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
authors: Suprit
authors: S
authors: Deshpande
authors: HIV Vaccine Translational Research Laboratory, NCR Biotech Science Cluster, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
authors: Shilpa
authors: S
authors: Patil
authors: HIV Vaccine Translational Research Laboratory, NCR Biotech Science Cluster, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
authors: Rajesh
authors: R
authors: Kumar
authors: National Institute of Communicable Diseases, Johannesburg, South Africa.
authors: Tandile
authors: T
authors: Hermanus
authors: YRG Care Center for AIDS Research & Education, Chennai, 600113, India.
authors: Kailapuri G
authors: KG
authors: Murugavel
authors: YRG Care Center for AIDS Research & Education, Chennai, 600113, India.
authors: Aylur K
authors: AK
authors: Srikrishnan
authors: YRG Care Center for AIDS Research & Education, Chennai, 600113, India.
authors: Suniti
authors: S
authors: Solomon
authors: National Institute of Communicable Diseases, Johannesburg, South Africa.
authors: Lynn
authors: L
authors: Morris
authors: HIV Vaccine Translational Research Laboratory, NCR Biotech Science Cluster, Translational Health Science and Technology Institute, Faridabad, Haryana, India. JBhattacharya@iavi.org.|International AIDS Vaccine Initiative, New York, NY, USA. JBhattacharya@iavi.org.
authors: Jayanta
authors: J
authors: Bhattacharya
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology*; D015483:HIV Antibodies / Q000737:chemistry / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D006801:Humans; D009154:Mutation; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D011134:Polysaccharides; D054299:env Gene Products, Human Immunodeficiency Virus
keywords: Envelope; HIV-1; Neutralizing antibody; Plasma