title: Dependence of RIG-I Nucleic Acid-Binding and ATP Hydrolysis on Activation of Type I Interferon Response.
abstract: Exogenous nucleic acids induce an innate immune response in mammalian host cells through activation of the retinoic acid-inducible gene I (RIG-I). We evaluated RIG-I protein for RNA binding and ATPase stimulation with RNA ligands to investigate the correlation with the extent of immune response through RIG-I activation in cells. RIG-I protein favored blunt-ended, double-stranded RNA (dsRNA) ligands over sticky-ended dsRNA. Moreover, the presence of the 5'-triphosphate (5'-ppp) moiety in dsRNA further enhanced binding affinity to RIG-I. Two structural motifs in RNA, blunt ends in dsRNA and 5'-ppp, stimulated the ATP hydrolysis activity of RIG-I. These structural motifs also strongly induced IFN expression as an innate immune response in cells. Therefore, we suggest that IFN induction through RIG-I activation is mainly determined by structural motifs in dsRNA that increase its affinity for RIG-I protein and stimulate ATPase activity in RIG-I.
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05902, Korea.
authors: Yu Mi
authors: YM
authors: Baek
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05902, Korea.
authors: Soojin
authors: S
authors: Yoon
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05902, Korea.
authors: Yeo Eun
authors: YE
authors: Hwang
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05902, Korea.
authors: Dong-Eun
authors: DE
authors: Kim
keywords: 5'-terminal triphosphate; ATP hydrolysis; Interferon induction; RNA binding; Retinoic acid inducible gene I (RIG-I)