title: Effects of Alpha-Synuclein on Primary Spinal Cord Neurons Associated with Apoptosis and CNTF Expression.
abstract: Spinal cord injury (SCI) often causes neurological deficits with poor recovery; the treatment, however, is far from satisfaction, and the mechanisms remain unclear. Using immunohistochemistry and western blotting analysis, we found α-synuclein (SNCA) was significantly up-regulated in the spinal caudal segment of rats subjected to spinal cord transection at 3 days post-operation. Moreover, the role of SNCA on neuronal growth and apoptosis in vitro was determined by using overexpressing and interfering SNCA recombined plasmid vectors, and the underlying mechanism was detected by QRT-PCR and western blotting. Spinal neurons transfected with SNCA-shRNA lentivirus gave rise to an optimal neuronal survival, while it results in cell apoptosis in SNCA-ORF group. In molecular level, SNCA silence induced the up-regulation of CNTF and down-regulation of Caspase7/9. Together, endogenous SNCA plays a crucial role in spinal neuronal survival, in which the underlying mechanism may be linked to the regulation both apoptotic genes (Caspase7/9) and CNTF. The present findings therefore provide novel insights into the role of SNCA in spinal cord and associated mechanism, which may provide novel cue for the treatment of SCI in future clinic trials.
authors: Department of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China.
authors: Guo-Ying
authors: GY
authors: Feng
authors: Institute of Neuroscience, Kunming Medical University, Kunming, 650031, China.
authors: Jia
authors: J
authors: Liu
authors: Department of Anesthesia, Institute of Neurological Disease, Translational Neuroscience Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
authors: You-Cui
authors: YC
authors: Wang
authors: Biomedical Engineering Research Center, Kunming Medical University, Kunming, 650031, China.
authors: Zhen-Yu
authors: ZY
authors: Wang
authors: Department of Anesthesia, Institute of Neurological Disease, Translational Neuroscience Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
authors: Yue
authors: Y
authors: Hu
authors: Department of Anesthesia, Institute of Neurological Disease, Translational Neuroscience Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
authors: Qing-Jie
authors: QJ
authors: Xia
authors: Department of Anesthesia, Institute of Neurological Disease, Translational Neuroscience Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
authors: Yang
authors: Y
authors: Xu
authors: Department of Anesthesia, Institute of Neurological Disease, Translational Neuroscience Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
authors: Fei-Fei
authors: FF
authors: Shang
authors: Institute of Neuroscience, Kunming Medical University, Kunming, 650031, China.
authors: Mei-Rong
authors: MR
authors: Chen
authors: Institute of Neuroscience, Kunming Medical University, Kunming, 650031, China. 56885606@qq.com.
authors: Fang
authors: F
authors: Wang
authors: Department of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China. zhouxue7239@163.com.
authors: Xue
authors: X
authors: Zhou
authors: Department of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China. 371854427@qq.com.|Institute of Neuroscience, Kunming Medical University, Kunming, 650031, China. 371854427@qq.com.|Department of Anesthesia, Institute of Neurological Disease, Translational Neuroscience Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. 371854427@qq.com.|Department of Histology and Neurobiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China. 371854427@qq.com.
authors: Ting-Hua
authors: TH
authors: Wang
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D017209:Apoptosis; D002470:Cell Survival; D020934:Ciliary Neurotrophic Factor / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D016086:Lentivirus / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D016366:Open Reading Frames / Q000235:genetics; D034741:RNA, Small Interfering / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism* / Q000473:pathology*; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology; D014162:Transfection; D019065:Virus Assembly; D051844:alpha-Synuclein / Q000378:metabolism*
chemical_list: D034961:Antigens, Nuclear; D020934:Ciliary Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; C546339:Rbfox3 protein, rat; D051844:alpha-Synuclein
keywords: Alpha-synuclein; Apoptotic gene; Ciliary neurotrophic factor; Primary spinal neurons; Spinal cord transection