title: Histomorphological Phenotyping of the Adult Mouse Brain.
abstract: This article describes a series of standard operating procedures for morphological phenotyping of the mouse brain using basic histology. Many histological studies of the mouse brain use qualitative approaches based on what the human eye can detect. Consequently, some phenotypic information may be missed. Here we describe a quantitative approach for the assessment of brain morphology that is simple and robust. A total of 78 measurements are made throughout the brain at specific and well-defined regions, including the cortex, the hippocampus, and the cerebellum. Experimental design and timeline considerations, including strain background effects, the importance of sectioning quality, measurement variability, and efforts to correct human errors are discussed. © 2016 by John Wiley & Sons, Inc.
authors: Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.
authors: Anna
authors: A
authors: Mikhaleva
authors: Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.|Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.|National Centre for Scientific Research, UMR7104, Illkirch, France.|National Institute of Health and Medical Research, U964, Illkirch, France.|University of Strasbourg, Illkirch, France.
authors: Meghna
authors: M
authors: Kannan
authors: Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.|National Centre for Scientific Research, UMR7104, Illkirch, France.|National Institute of Health and Medical Research, U964, Illkirch, France.|University of Strasbourg, Illkirch, France.
authors: Christel
authors: C
authors: Wagner
authors: Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.|Institute of Genetics and Molecular and Cellular Biology, Illkirch, France.|National Centre for Scientific Research, UMR7104, Illkirch, France.|National Institute of Health and Medical Research, U964, Illkirch, France.|University of Strasbourg, Illkirch, France.
authors: Binnaz
authors: B
authors: Yalcin
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D006652:Histological Techniques / Q000379:methods*; D051379:Mice / Q000033:anatomy & histology*
keywords: brain morphology; histology; mouse; phenotyping