title: Terpenoid bioactive compound from Streptomyces rochei (M32): taxonomy, fermentation and biological activities.
abstract: The present study emphasized the production of biologically active terpenoid compound from Streptomyces rochei M32, which was isolated from Western Ghats ecosystem, South India. The presence of resistant genes like mecA, vanA of Staphylococcus aureus and bla SHV, bla TEM of Pseudomonas aeruginosa was confirmed by molecular studies. The isolated compound from Streptomyces rochei M32 inhibited wide range of standard and clinical drug resistant pathogens and enteric pathogens. The rice bran supplemented basal medium influenced the active compound production on 8th day of fermentation and yielded 1875 mg of crude extract from 10 g of rice bran substrate. Purification and characterization of crude ethyl acetate extract was achieved by preparative thin layer chromatography. The active fraction was identified as terpenoid class compound by chemical screening. Based on the results of spectral studies (NMR, LC-MS, FTIR, etc.), the active compound was tentatively identified as 1, 19-bis (3-hydroxyazetidin-1-yl) nonadeca-5, 14-diene-1, 8, 12, 19-tetraone with molecular weight 462.41 g/mol. Minimum inhibitory concentration value ranges between 7.6 and 31.2 µg/mL against test organisms was observed. The cytotoxicity results on cervical cancer (HeLa) cell line showed IC50 value of 2.034 µg/mL. The corresponding compound is not previously reported from any microbial resources.
authors: Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu, 636 011, India.
authors: Raasaiyah
authors: R
authors: Pazhanimurugan
authors: Centre for Drug Discovery and Development, Sathyabama University, Jeppiar Nagar, Chennai, Tamil Nadu, 600 119, India.
authors: Manikkam
authors: M
authors: Radhakrishnan
authors: Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu, 636 011, India.
authors: Thangavel
authors: T
authors: Shanmugasundaram
authors: Centre for Drug Discovery and Development, Sathyabama University, Jeppiar Nagar, Chennai, Tamil Nadu, 600 119, India.
authors: Venugopal
authors: V
authors: Gopikrishnan
authors: Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu, 636 011, India. actinobalaguru@gmail.com.
authors: Ramasamy
authors: R
authors: Balagurunathan
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D005285:Fermentation; D006367:HeLa Cells; D006801:Humans; D008826:Microbial Sensitivity Tests; D012275:Oryza / Q000382:microbiology; D013302:Streptomyces / Q000145:classification* / Q000302:isolation & purification; D013729:Terpenes / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D013729:Terpenes
keywords: Actinobacteria; Chromatography; Cytotoxicity; Resistant; Streptomyces; Terpenoid