title: Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery.
abstract: During reconstructive bone surgeries, supraphysiological amounts of growth factors are empirically loaded onto scaffolds to promote successful bone fusion. Large doses of highly potent biological agents are required due to growth factor instability as a result of rapid enzymatic degradation as well as carrier inefficiencies in localizing sufficient amounts of growth factor at implant sites. Hence, strategies that prolong the stability of growth factors such as BMP-2/NELL-1, and control their release could actually lower their efficacious dose and thus reduce the need for larger doses during future bone regeneration surgeries. This in turn will reduce side effects and growth factor costs. Self-assembled PECs have been fabricated to provide better control of BMP-2/NELL-1 delivery via heparin binding and further potentiate growth factor bioactivity by enhancing in vivo stability. Here we illustrate the simplicity of PEC fabrication which aids in the delivery of a variety of growth factors during reconstructive bone surgeries.
authors: Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore; doslwmr@nus.edu.sg.
authors: Raymond
authors: R
authors: Wing Moon Lam
authors: Centre for Research in Medical Devices (CÚRAM), National University of Ireland Galway.
authors: Sunny Akogwu
authors: SA
authors: Abbah
authors: Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore.
authors: Wang
authors: W
authors: Ming
authors: Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore.
authors: Mathanapriya
authors: M
authors: Naidu
authors: Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore.
authors: Felly
authors: F
authors: Ng
authors: Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore.
authors: Hu
authors: H
authors: Tao
authors: Department of Bioengineering, Faculty of Engineering, National University of Singapore; Tissue Engineering Program, National University of Singapore.
authors: James
authors: J
authors: Goh Cho Hong
authors: Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, University of California, Los Angeles.
authors: Kang
authors: K
authors: Ting
authors: Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore; Tissue Engineering Program, National University of Singapore.
authors: Wong
authors: W
authors: Hee Kit
mesh_terms: D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D001861:Bone Regeneration / Q000502:physiology*; D006493:Heparin / Q000737:chemistry; D010012:Osteogenesis; D000071228:Polyelectrolytes
chemical_list: D055396:Bone Morphogenetic Protein 2; D000071228:Polyelectrolytes; D006493:Heparin