title: Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis.
abstract: Intestinal epithelial cells continuously migrate and mature along crypt-villus axis (CVA), while the changes in energy metabolism during maturation are unclear in neonates. The present study was conducted to test the hypothesis that the energy metabolism in intestinal epithelial cells would be changed during maturation along CVA in neonates. Eight 21-day-old suckling piglets were used. Intestinal epithelial cells were isolated sequentially along CVA, and proteomics was used to analyze the changes in proteins expression in epithelial cells along CVA. The identified differentially expressed proteins were mainly involved in cellular process, metabolic process, biological regulation, pigmentation, multicellular organizational process and so on. The energy metabolism in intestinal epithelial cells of piglets was increased from the bottom of crypt to the top of villi. Moreover, the expression of proteins related to the metabolism of glucose, most of amino acids, and fatty acids was increased in intestinal epithelial cells during maturation along CVA, while the expression of proteins related to glutamine metabolism was decreased from crypt to villus tip. The expression of proteins involved in citrate cycle was also increased intestinal epithelial cells during maturation along CVA. Moreover, dietary supplementation with different energy sources had different effects on intestinal structure of weaned piglets.
authors: Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha, China.|Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center of Healthy Breeding Livestock &Poultry, Human Engineering &Research Center of Animal &Poultry Science, Key Lab Agroecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China.|Fujian Aonong Bio-Technology Co., Ltd., Xiamen, China.
authors: Huansheng
authors: H
authors: Yang
authors: Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center of Healthy Breeding Livestock &Poultry, Human Engineering &Research Center of Animal &Poultry Science, Key Lab Agroecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China.
authors: Xiaocheng
authors: X
authors: Wang
authors: Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center of Healthy Breeding Livestock &Poultry, Human Engineering &Research Center of Animal &Poultry Science, Key Lab Agroecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China.
authors: Xia
authors: X
authors: Xiong
authors: Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha, China.|Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center of Healthy Breeding Livestock &Poultry, Human Engineering &Research Center of Animal &Poultry Science, Key Lab Agroecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China.
authors: Yulong
authors: Y
authors: Yin
mesh_terms: D000596:Amino Acids / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002952:Citric Acid Cycle / Q000235:genetics; D015536:Down-Regulation; D004734:Energy Metabolism / Q000235:genetics*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005227:Fatty Acids / Q000235:genetics / Q000378:metabolism; D006019:Glycolysis / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology / Q000473:pathology; D010455:Peptides / Q000032:analysis / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D013552:Swine; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D010455:Peptides; D011506:Proteins