title: Oral immunisation of mice with transgenic rice calli expressing cholera toxin B subunit fused to consensus dengue cEDIII antigen induces antibodies to all four dengue serotypes.
abstract: Dengue virus (DENV) infection is an emerging global health threat. DENV consists of four distinct serotypes, necessitating a tetravalent vaccine. In this study, expression of consensus envelope protein domain III (cEDIII) fused to cholera toxin B subunit (CTB) in transgenic rice calli was improved using the luminal binding protein BiP at the N-terminus and the SEKDEL signal sequences at the C-terminus, targeting the recombinant protein to endoplasmic reticulum (ER). We found that the fusion protein showed higher levels of expression when compared to the fusion proteins using rice amylase 3D (RAmy3D) or CTB native signal sequence only. The CTB-cEDIII fusion protein was evaluated as an oral dengue vaccine candidate in mice. Serotype specific systemic IgG antibodies and specific IgA response in feces were detected and furthermore, T cell proliferation and high frequency antibody-secreting B cells were detected in the spleen. These results suggest the possible use of plant-based dengue tetravalent vaccine targeted to the mucosal immune system for induction of systemic and mucosal immune responses to DENV infection.
authors: Department of Molecular Biology, Chonbuk National University, Jeonju, South Korea.|Institute for Infection and Immunity, St George's University of London, London, UK.
authors: Mi-Young
authors: MY
authors: Kim
authors: Department of Bioactive Material Sciences, Chonbuk National University, Jeonju, South Korea.
authors: Byeong-Young
authors: BY
authors: Kim
authors: Department of Molecular Biology, Chonbuk National University, Jeonju, South Korea.
authors: Sun-Mi
authors: SM
authors: Oh
authors: Institute for Infection and Immunity, St George's University of London, London, UK.
authors: Rajko
authors: R
authors: Reljic
authors: Department of Molecular Biology, Chonbuk National University, Jeonju, South Korea.|Department of Bioactive Material Sciences, Chonbuk National University, Jeonju, South Korea.|Research Center of Bioactive Materials, Chonbuk National University, Jeonju, South Korea.
authors: Yong-Suk
authors: YS
authors: Jang
authors: Department of Molecular Biology, Chonbuk National University, Jeonju, South Korea. mskyang@jbnu.ac.kr.|Department of Bioactive Material Sciences, Chonbuk National University, Jeonju, South Korea. mskyang@jbnu.ac.kr.|Research Center of Bioactive Materials, Chonbuk National University, Jeonju, South Korea. mskyang@jbnu.ac.kr.
authors: Moon-Sik
authors: MS
authors: Yang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D002772:Cholera Toxin / Q000235:genetics; D003715:Dengue / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D051379:Mice; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000276:immunology*; D065288:Serogroup
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D002772:Cholera Toxin
keywords: Dengue; Endoplasmic reticulum; Oral vaccine; Transgenic rice (Oryza sativa L.)