title: Islet Insulin Secretion Measurements in the Mouse.
abstract: This article describes detailed protocols for in vitro measurements of insulin function and secretion in isolated mouse islets for the analysis of glucose homeostasis. We specify a method of enzyme digestion and hand picking to isolate and release the greatest number of high quality islets from the pancreas of the mouse. We describe an effective method for generating dynamic measurements of insulin secretion using a perifusion assay including a detailed protocol for constructing a peristaltic pump and tubing assembly. In addition we describe an alternative and simple technique for measuring insulin secretion using static incubation of isolated islets. © 2016 by John Wiley & Sons, Inc.
authors: Mammalian Genetics Unit, Medical Research Council Harwell, Harwell Science and Innovation Campus, Oxfordshire, United Kingdom.
authors: Alison
authors: A
authors: Hugill
authors: Department of Medical Electrophysiology, Fukushima Medical University, School of Medicine, Fukushima, Japan.
authors: Kenju
authors: K
authors: Shimomura
authors: Mammalian Genetics Unit, Medical Research Council Harwell, Harwell Science and Innovation Campus, Oxfordshire, United Kingdom.
authors: Roger D
authors: RD
authors: Cox
mesh_terms: D000818:Animals; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice / Q000378:metabolism*; D010827:Physiology / Q000379:methods*
chemical_list: D007328:Insulin
keywords: dynamic measurements; insulin secretion; islets of Langerhans; perifusion; static measurements