title: Intracellular uptake study of radiolabeled anticancer drug and impedimetric detection of its interaction with DNA.
abstract: Topoisomerase I inhibitor topotecan (TPT) is the only single-agent therapy certified for the remedy of repetitive small cell lung cancer (SCLC). In this study, TPT was labeled with (131)I via iodogen method and its quality control was determined using thin layer radiochromatography and paper electrophoresis methods. Intracellular uptake study was carried out with human lung adenocarcinoma cell line (A-549) and human lung fibroblast cell line (WI-38). The interaction of (131)I-TPT with healthy DNA and cancer DNA was also investigated using single-use sensor technology combined with electrochemical impedance spectroscopy (EIS). The change at the charge transfer resistance (Rct) obtained before/after interaction was evaluated. Similar to the results of intracellular uptake study, it was found that (131)I-TPT could more interact with the cancer DNA than healthy DNA according to the impedimetric results. (131)I-TPT is promising in terms of a new nuclear imaging agent for lung cancer.
authors: Department of Material Science and Engineering, Ege University, Bornova, 35100 Izmir, Turkey; Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova, 35100 Izmir, Turkey; Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova, 35100 Izmir, Turkey.
authors: Mustafa
authors: M
authors: Top
authors: Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova, 35100 Izmir, Turkey.
authors: Ozge
authors: O
authors: Er
authors: Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova, 35100 Izmir, Turkey.
authors: Gulsah
authors: G
authors: Congur
authors: Department of Material Science and Engineering, Ege University, Bornova, 35100 Izmir, Turkey; Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Bornova, 35100 Izmir, Turkey. Electronic address: arzum.erdem@ege.edu.tr.
authors: Arzum
authors: A
authors: Erdem
authors: Department of Material Science and Engineering, Ege University, Bornova, 35100 Izmir, Turkey; Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova, 35100 Izmir, Turkey. Electronic address: fatma.yurt.lambrecht@ege.edu.tr.
authors: Fatma Yurt
authors: FY
authors: Lambrecht
mesh_terms: D000072283:A549 Cells; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001692:Biological Transport; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D058266:Dielectric Spectroscopy; D006801:Humans; D007457:Iodine Radioisotopes; D008175:Lung Neoplasms / Q000235:genetics; D019772:Topotecan / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D007457:Iodine Radioisotopes; D019772:Topotecan; D004247:DNA
keywords: (131)I; Drug-DNA interaction; Impedimetric Detection; Small cell lung cancer; Topotecan