title: The paracrine immunomodulatory interactions between the human dental pulp derived mesenchymal stem cells and CD4 T cell subsets.
abstract: Mesenchymal stem cells (MSCs) have strong immunomodulatory properties, however these properties may show some differences according to the tissue type of their isolate. In this study we investigated the paracrine interactions between human DP derived MSCs (hDP-MSCs) and the CD4+T helper cell subsets to establish their immunomodulatory mechanisms. We found that the CD4+-Tbet+(Th1) and CD4+-Gata3+(Th2) cells were suppressed by the hDP-MSCs, but the CD4+-Stat3+(Th17) and CD4+-CD25+-FoxP3+(Treg) cells were stimulated. The expressions of T cell specific cytokines interferon gamma (IFN-g), interleukin (IL)-4 and IL-17a decreased, but IL-10 and transforming growth factor beta-1 (TGF-b1) increased with the hDP-MSCs. The expressions of indoleamine-pyrrole 2,3-dioxygenase (IDO), prostaglandin E2 (PGE2), soluble human leukocyte antigen G (sHLA-G) derived from hDP-MSCs slightly increased, but hepatocyte growth factor (HGF) significantly increased in the co-culture groups. According to our findings, the hDP-MSCs can suppress the Th1 and Th2 subsets but stimulate the Th17 and Treg subsets. The Stat3 expression of Th17 cells may have been stimulated by the HGF, and thus the pro-inflammatory Th17 cells may have altered into the immunosuppressive regulatory Th17 cells. Further prospective studies are needed to confirm our findings.
authors: Ege University, Institute of Health Sciences, Department of Stem Cell, 35100 Izmir, Turkey. Electronic address: alpertungaozdemir@gmail.com.
authors: Alper Tunga
authors: AT
authors: Özdemir
authors: Manisa State Hospital, Department of Allergy and Clinical Immunology, 45000 Manisa, Turkey.
authors: Rabia Bilge
authors: RB
authors: Özgül Özdemir
authors: Celal Bayar University, Medical School, Department of Internal Medicine, Division of Allergy and Clinical Immunology, 45050 Manisa, Turkey.
authors: Cengiz
authors: C
authors: Kırmaz
authors: Eskişehir Osmangazi University, Cellular Therapy and Stem Cell Production Application and Research Center, 26480 Eskişehir, Turkey.
authors: Ayla Eker
authors: AE
authors: Sarıboyacı
authors: Kocaeli University, Stem Cell and Gene Therapies Research and Application Center, 41000 Kocaeli, Turkey.
authors: Zehra Seda
authors: ZS
authors: Ünal Halbutoğlları
authors: Kocaeli University, Stem Cell and Gene Therapies Research and Application Center, 41000 Kocaeli, Turkey.
authors: Ceren
authors: C
authors: Özel
authors: Liv Hospital, Center of Regenerative Medicine and Stem Cell Research, 34000 Istanbul, Turkey; Istinye University, Medical School, Department of Histology and Embryology, 34000 Istanbul, Turkey.
authors: Erdal
authors: E
authors: Karaöz
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D003782:Dental Pulp / Q000473:pathology; D015232:Dinoprostone / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000378:metabolism; D050990:GATA3 Transcription Factor / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D056747:Immunomodulation; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D019899:Paracrine Communication; D050796:STAT3 Transcription Factor / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D000098815:T-bet Transcription Factor
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; C066855:HGF protein, human; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D050796:STAT3 Transcription Factor; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D017228:Hepatocyte Growth Factor; D015232:Dinoprostone
keywords: CD(4) T cell; FoxP3; Gata3; Hepatocyte growth factor; Immunomodulation; Interleukin; Mesenchymal stem cell; Stat3; T-bet