title: Establishing an Infrastructure for High-Throughput Short-Interfering RNA Screening.
abstract: RNA interference (RNAi) is a readily available research tool that can be used to accelerate the identification and functional validation of a multitude of new candidate drug targets by experimentally perturbing gene expression and function. High-throughput RNAi technology using libraries of short-interfering RNA (siRNA) makes it possible to rapidly identify genes and biomarkers associated with biological processes such as diseases or a cellular response to therapy. Thus, RNAi-based screening is an extremely powerful technology that can provide tremendous insights into the mechanisms of action and contexts of vulnerability of a particular drug treatment. This chapter describes the infrastructure requirements needed to successfully perform HT-RNAi screening. Information on the methodology, instrumentation, experimental design, and workflow aspects is provided, as well as insights on how to successfully implement a high-throughput RNAi screen.
authors: Cancer and Cell Biology Division (CCB), Translational Genomics Research Institute (TGen), 445 N. Fifth Street, Phoenix, AZ, 85004, USA. hyin@tgen.org.
authors: Hongwei
authors: H
authors: Yin
authors: Cancer and Cell Biology Division (CCB), Translational Genomics Research Institute (TGen), 445 N. Fifth Street, Phoenix, AZ, 85004, USA.
authors: Chris
authors: C
authors: Sereduk
authors: Cancer and Cell Biology Division (CCB), Translational Genomics Research Institute (TGen), 445 N. Fifth Street, Phoenix, AZ, 85004, USA.
authors: Nanyun
authors: N
authors: Tang
mesh_terms: D002460:Cell Line; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D034622:RNA Interference; D034741:RNA, Small Interfering; D015203:Reproducibility of Results; D057188:Workflow
chemical_list: D034741:RNA, Small Interfering
keywords: Functional genomics; HTS equipment; High-throughput; RNA interference; RNAi; Screening workflow; siRNA