title: Acute respiratory changes and pulmonary inflammation involving a pathway of TGF-β1 induction in a rat model of chlorine-induced lung injury.
abstract: We investigated acute and delayed respiratory changes after inhalation exposure to chlorine (Cl2) with the aim to understand the pathogenesis of the long-term sequelae of Cl2-induced lung-injury. In a rat model of nose-only exposure we analyzed changes in airway hyperresponsiveness (AHR), inflammatory responses in airways, expression of pro-inflammatory markers and development of lung fibrosis during a time-course from 5h up to 90days after a single inhalation of Cl2. A single dose of dexamethasone (10mg/kg) was administered 1h following Cl2-exposure. A 15-min inhalation of 200ppm Cl2 was non-lethal in Sprague-Dawley rats. At 24h post exposure, Cl2-exposed rats displayed elevated numbers of leukocytes with an increase of neutrophils and eosinophils in bronchoalveolar lavage (BAL) and edema was shown both in lung tissue and the heart. At 24h, the inflammasome-associated cytokines IL-1β and IL-18 were detected in BAL. Concomitant with the acute inflammation a significant AHR was detected. At the later time-points, a delayed inflammatory response was observed together with signs of lung fibrosis as indicated by increased pulmonary macrophages, elevated TGF-β expression in BAL and collagen deposition around airways. Dexamethasone reduced the numbers of neutrophils in BAL at 24h but did not influence the AHR. Inhalation of Cl2 in rats leads to acute respiratory and cardiac changes as well as pulmonary inflammation involving induction of TGF-β1. The acute inflammatory response was followed by sustained macrophage response and lack of tissue repair. It was also found that pathways apart from the acute inflammatory response contribute to the Cl2-induced respiratory dysfunction.
authors: Swedish Defence Research Agency, CBRN Defence and Security, Umeå, Sweden.
authors: Elisabeth
authors: E
authors: Wigenstam
authors: Swedish Defence Research Agency, CBRN Defence and Security, Umeå, Sweden.
authors: Linda
authors: L
authors: Elfsmark
authors: Swedish Defence Research Agency, CBRN Defence and Security, Umeå, Sweden.
authors: Bo
authors: B
authors: Koch
authors: Swedish Defence Research Agency, CBRN Defence and Security, Umeå, Sweden; Department of Public Health and Clinical Medicine, Unit of Respiratory Medicine, Umeå University, Umeå, Sweden.
authors: Anders
authors: A
authors: Bucht
authors: Swedish Defence Research Agency, CBRN Defence and Security, Umeå, Sweden. Electronic address: sofia.jonasson@foi.se.
authors: Sofia
authors: S
authors: Jonasson
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000188:drug therapy / Q000473:pathology; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000139:chemically induced* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid; D002713:Chlorine / Q000008:administration & dosage / Q000633:toxicity*; D003907:Dexamethasone / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D019570:Inhalation Exposure; D011014:Pneumonia / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*
chemical_list: D053773:Transforming Growth Factor beta1; D002713:Chlorine; D003907:Dexamethasone
keywords: Airway hyperresponsiveness; Chemical-induced lung injury; Chlorine; Inflammation; Respiratory mechanics