title: Inhibitory Components fromGlycosmis stenocarpaon Pepper Mild Mottle Virus.
abstract: The goal of this study was to identify a source of natural plant compounds with inhibitory activity against pepper mild mottle virus (PMMoV). We showed, using a half-leaf assay, that murrayafoline-A (1) and isomahanine (2) isolated from the aerial parts ofGlycosmis stenocarpahave inhibitory activity against PMMoV through curative, inactivation, and protection effects. Using a leaf-disk assay, we confirmed that2inhibited virus replication inNicotiana benthamiana. Using electron microscopy, we found that a mixture of the virus with2resulted in damage to the rod-shaped virus.
authors: Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Republic of Korea.|Korea Atomic Energy Research Institute, Jeongeup 56143, Republic of Korea.
authors: Jang Hoon
authors: JH
authors: Kim
authors: Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Republic of Korea.
authors: Ju-Yeon
authors: JY
authors: Yoon
authors: Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Republic of Korea.
authors: Sun Jung
authors: SJ
authors: Kwon
authors: Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Republic of Korea.
authors: In Sook
authors: IS
authors: Cho
authors: Department of Bioactive Products, Vietnam Academy of Science and Technology, Hanoi 122100, Vietnam.
authors: Nguyen Manh
authors: NM
authors: Cuong
authors: Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Republic of Korea.
authors: Seung-Kook
authors: SK
authors: Choi
authors: College of Pharmacy, Chungnam National University, Daejeon 34134, Republic of Korea.
authors: Young Ho
authors: YH
authors: Kim
authors: Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Republic of Korea.
authors: Gug Seoun
authors: GS
authors: Choi
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D010935:Plant Diseases / Q000821:virology; D010936:Plant Extracts / Q000494:pharmacology*; D027821:Rutaceae / Q000737:chemistry*; D014026:Nicotiana / Q000821:virology; D017898:Tobamovirus / Q000187:drug effects* / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts
keywords: Glycosmis stenocarpa; Pepper mild mottle virus; antiviral agent; electron miscroscopy; isomahanine