title: The protective effect of Citrus limon essential oil on hepatotoxicity and nephrotoxicity induced by aspirin in rats.
abstract: Citrus limon is a member of the large Rutaceae family characterized by its therapeutic proprieties and has been widely used in traditional medicine to treat various diseases. This study investigates the protective effect of Citrus limon essential oil against a high dose of aspirin-induced acute liver and kidney damage in female Wistar albino rats. Twenty-eight adult female Wistar rats were divided into 4 groups of 7 each: (1) a control group; (2) a group of rats which was kept untreated for 56days then treated with aspirin (A) (600mg/kg) for 4 days; (3) a group fed with essential oil of Citrus limon for 56days then (A) for 4 days; and (4) a group of rats receiving essential oil of Citrus limon for 56 days, then given NaCl for 4 days. Estimations of biochemical parameters in blood were determined. Lipid peroxidation levels (TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidas (GPx) activities in liver and kidney was determined. A histopathological study was done. Under our experimental conditions, aspirin induced an increase of serum biochemical parameters and it resulted in an oxidative stress in both liver and kidney. This was evidenced by significant increase in TBARS in liver and kidney by 108% and 55%, respectively, compared to control. On the other hand, a decrease in the activities of SOD by 78% and 53%, CAT by 53% and 78%, and GPx by 78% and 51% in liver and kidney, respectively. Administration of EOC to rats attenuated the induced an effect of the high dose of aspirin induced in the afore mentioned serum biochemical parameters. In conclusion, our data suggest that treatment with essential oil of Citrus limon prevented the liver and kidney damage induced by aspirin.
authors: Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences Sfax, Road Soukra km 3.5, PB no 1171-3000, Sfax, Tunisie; Laboratory Animal Eco Physiology, Faculty of Sciences of Gafsa, 2112, Tunisie; EA 1274, Faculté de Médecine et Sciences de la Santé, Université de Bretagne Occidentale, 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, France. Electronic address: bouzenna.hafsia@hotmail.fr.
authors: Hafsia
authors: H
authors: Bouzenna
authors: Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences Sfax, Road Soukra km 3.5, PB no 1171-3000, Sfax, Tunisie; Laboratory Animal Eco Physiology, Faculty of Sciences of Gafsa, 2112, Tunisie.
authors: Sabah
authors: S
authors: Dhibi
authors: Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences Sfax, Road Soukra km 3.5, PB no 1171-3000, Sfax, Tunisie; Laboratory Animal Eco Physiology, Faculty of Sciences of Gafsa, 2112, Tunisie.
authors: Noura
authors: N
authors: Samout
authors: Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences Sfax, Road Soukra km 3.5, PB no 1171-3000, Sfax, Tunisie; Laboratory Animal Eco Physiology, Faculty of Sciences of Gafsa, 2112, Tunisie.
authors: Ilhem
authors: I
authors: Rjeibi
authors: EA 1274, Faculté de Médecine et Sciences de la Santé, Université de Bretagne Occidentale, 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, France.
authors: Hélène
authors: H
authors: Talarmin
authors: Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences Sfax, Road Soukra km 3.5, PB no 1171-3000, Sfax, Tunisie.
authors: Abdelfattah
authors: A
authors: Elfeki
authors: Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences Sfax, Road Soukra km 3.5, PB no 1171-3000, Sfax, Tunisie; Laboratory Animal Eco Physiology, Faculty of Sciences of Gafsa, 2112, Tunisie.
authors: Najla
authors: N
authors: Hfaiedh
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity; D001241:Aspirin / Q000633:toxicity*; D002957:Citrus; D005260:Female; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D035261:Plant Components, Aerial; D018515:Plant Leaves; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D009822:Oils, Volatile; D020011:Protective Agents; D001241:Aspirin
keywords: Aspirin; Citrus limon; Kidney; Liver; Oxidative stress