title: Angiogenic Effects of Dimeric Dipeptide Mimetic of Loop 4 of Nerve Growth Factor.
abstract: Angiogenic action of compound GK-2, a dimeric dipeptide mimetic of loop 4 of nerve growth factor (NGF), was studied in in vitro and in vivo experiments. Experiments on human endothelial cell culture HUVEC showed that compound GK-2 significantly (p<0.05) stimulated the initial stage of angiogenesis, and its angiogenic activity was not inferior to the reference neurotrophin NGF. In experiments with hindlimb ischemia modeled in rats, GK-2 (1 mg/kg intraperitoneally for 14 days) significantly increased the total length of capillary vessels (p<0.003) and the number of vessels per 1 mm2 ischemic tissue (p<0.001) in comparison with the control. Our findings indicate that under experimental conditions compound GK-2 exhibits not only angiogenic, but also anti-ischemic activity.
authors: V. V. Zakusov Institute of Pharmacology, Moscow, Russia. sak-538@yandex.ru.
authors: S A
authors: SA
authors: Kryzhanovskii
authors: V. V. Zakusov Institute of Pharmacology, Moscow, Russia.
authors: T A
authors: TA
authors: Antipova
authors: V. V. Zakusov Institute of Pharmacology, Moscow, Russia.
authors: I B
authors: IB
authors: Tsorin
authors: V. V. Zakusov Institute of Pharmacology, Moscow, Russia.
authors: E S
authors: ES
authors: Pekeldina
authors: V. V. Zakusov Institute of Pharmacology, Moscow, Russia.
authors: V N
authors: VN
authors: Stolyaruk
authors: V. V. Zakusov Institute of Pharmacology, Moscow, Russia.
authors: S V
authors: SV
authors: Nikolaev
authors: V. V. Zakusov Institute of Pharmacology, Moscow, Russia.
authors: A V
authors: AV
authors: Sorokina
authors: V. V. Zakusov Institute of Pharmacology, Moscow, Russia.
authors: T A
authors: TA
authors: Gudasheva
authors: V. V. Zakusov Institute of Pharmacology, Moscow, Russia.
authors: S B
authors: SB
authors: Seredenin
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D004151:Dipeptides / Q000737:chemistry / Q000627:therapeutic use*; D006614:Hindlimb / Q000473:pathology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007511:Ischemia / Q000188:drug therapy*; D008297:Male; D020932:Nerve Growth Factor / Q000737:chemistry*; D051381:Rats
chemical_list: D043925:Angiogenesis Inducing Agents; D004151:Dipeptides; D020932:Nerve Growth Factor
keywords: angiogenesis; dimeric dipeptide mimetic; human endothelial cell culture (HUVEC); limb ischemia; nerve growth factor