title: Prevalence and Characteristics of Salmonella and Campylobacter in Retail Poultry Meat in Japan.
abstract: This study was performed to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of Salmonella enterica subsp. enterica and Campylobacter spp. in poultry meat, and to analyze the association of genetic types of these bacteria with their geographical distribution and antimicrobial resistance profiles. Salmonella and Campylobacter isolates have been detected, respectively, in 54 and 71 samples out of 100 samples tested. Nine Salmonella serotypes were found, including S. enterica subsp. enterica serovar Infantis (33%), Schwarzengrund (12%), Manhattan (9%), and others. Campylobacter jejuni and C. coli were detected in 64 (64%) and 14 (14%) samples, respectively. S. enterica subsp. enterica isolates were very frequently resistant to tetracycline (78.3%) and streptomycin (68.3%). Many C. jejuni and C. coli isolates were resistant to sulfamethoxazole/trimethoprim (90.5%), nalidixic acid (47.3%), ampicillin (45.9%), and ciprofloxacin (40.5%). Cluster analysis was performed for the Salmonella isolates using pulsed-field gel electrophoresis (PFGE) data. For Campylobacter isolates, the cluster analysis was based on both PFGE and comparative genomic fingerprinting. The molecular typing results were compared with the information about antimicrobial resistance and geographical locations in which the poultry meat was produced. This analysis revealed that C. jejuni strains with a particular genotype and antimicrobial resistance profile are spreading in specific areas of Japan.
authors: Department of Microbiology, Kanagawa Prefectural Institute of Public Health.
authors: Ichiro
authors: I
authors: Furukawa
authors: Department of Microbiology, Kanagawa Prefectural Institute of Public Health.
authors: Tomoe
authors: T
authors: Ishihara
authors: Department of Regional Hygiene Inspection, Kanagawa Prefectural Institute of Public Health.
authors: Hiroshi
authors: H
authors: Teranishi
authors: Division of Planning and Administration Office, Akita Research Center for Public Health and Environment.
authors: Shioko
authors: S
authors: Saito
authors: Division of Hygiene, Akita Research Center for Public Health and Environment.
authors: Jun
authors: J
authors: Yatsuyanagi
authors: Division of Hygiene, Akita Research Center for Public Health and Environment.
authors: Eriko
authors: E
authors: Wada
authors: Division of Hygiene, Akita Research Center for Public Health and Environment.
authors: Yuko
authors: Y
authors: Kumagai
authors: Division of Hygiene, Akita Research Center for Public Health and Environment.
authors: Shiho
authors: S
authors: Takahashi
authors: Division of Hygiene, Akita Research Center for Public Health and Environment.
authors: Takayuki
authors: T
authors: Konno
authors: Division of Hygiene, Akita Research Center for Public Health and Environment.
authors: Hiroko
authors: H
authors: Kashio
authors: Saitama City Institute of Health Science and Research.
authors: Akihiko
authors: A
authors: Kobayashi
authors: Saitama City Institute of Health Science and Research.
authors: Naoki
authors: N
authors: Kato
authors: Microbiology group, Shiga Prefectural Institute of Public Health.
authors: Ken-Ichi
authors: KI
authors: Hayashi
authors: Microbiology group, Shiga Prefectural Institute of Public Health.
authors: Keisuke
authors: K
authors: Fukushima
authors: Microbiology group, Shiga Prefectural Institute of Public Health.
authors: Kazuhiko
authors: K
authors: Ishikawa
authors: Department of Health Science, Fukuoka Institute of Health and Environmental Sciences.
authors: Kazumi
authors: K
authors: Horikawa
authors: Department of Health Science, Fukuoka Institute of Health and Environmental Sciences.
authors: Akira
authors: A
authors: Oishi
authors: Department of Bacteriology I, National Institute of Infectious Diseases.
authors: Hidemasa
authors: H
authors: Izumiya
authors: Division of Microbiology, National Institutes of Health Sciences.
authors: Takahiro
authors: T
authors: Ohnishi
authors: Department of Food and Life Sciences, The Graduate School of Life and Environmental Sciences, Azabu University.
authors: Yoshiko
authors: Y
authors: Konishi
authors: Department of Microbiology, Kanagawa Prefectural Institute of Public Health.
authors: Toshiro
authors: T
authors: Kuroki
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016123:Campylobacter jejuni / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005506:Food Contamination; D007564:Japan; D008460:Meat / Q000382:microbiology*; D058889:Molecular Typing; D058974:Phylogeography; D011200:Poultry / Q000382:microbiology*; D015995:Prevalence; D012475:Salmonella / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Campylobacter; Salmonella; antimicrobial resistance; genotype; poultry meat