title: High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans.
abstract: RNA interference (RNAi) is a process in which double-stranded RNA (dsRNA) molecules mediate the inhibition of gene expression. RNAi in C. elegans can be achieved by simply feeding animals with bacteria expressing dsRNA against the gene of interest. This "feeding" method has made it possible to conduct genome-wide RNAi experiments for the systematic knockdown and subsequent investigation of almost every single gene in the genome. Historically, these genome-scale RNAi screens have been labor and time intensive. However, recent advances in automated, high-throughput methodologies have allowed the development of more rapid and efficient screening protocols. In this report, we describe a fast and efficient, liquid-based method for genome-wide RNAi screening.
authors: Departments of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, 15224, PA, USA.|Division of Newborn Medicine, Children's Hospital of Pittsburgh of UPMC, 4401 Penn Ave., Pittsburgh, 15224, PA, USA.
authors: Linda P
authors: LP
authors: O'Reilly
authors: Departments of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, 15224, PA, USA.|Division of Newborn Medicine, Children's Hospital of Pittsburgh of UPMC, 4401 Penn Ave., Pittsburgh, 15224, PA, USA.
authors: Ryan R
authors: RR
authors: Knoerdel
authors: Departments of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, 15224, PA, USA.|Division of Newborn Medicine, Children's Hospital of Pittsburgh of UPMC, 4401 Penn Ave., Pittsburgh, 15224, PA, USA.|Departments of Pediatrics, Washington University School of Medicine, 660 South Euclid Ave., St Louis, 63110, MO, USA.
authors: Gary A
authors: GA
authors: Silverman
authors: Departments of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, 15224, PA, USA. paksc@upmc.edu.|Division of Newborn Medicine, Children's Hospital of Pittsburgh of UPMC, 4401 Penn Ave., Pittsburgh, 15224, PA, USA. paksc@upmc.edu.|Departments of Pediatrics, Washington University School of Medicine, 660 South Euclid Ave., St Louis, 63110, MO, USA. paksc@upmc.edu.
authors: Stephen C
authors: SC
authors: Pak
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D049751:Genome, Helminth; D057166:High-Throughput Screening Assays / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D034622:RNA Interference
keywords: Arrayscan; C. elegans; Genome wide; High-throughput screening; RNAi