title: Occurrence of viral DNA in paired samples of corneal rim and cornea preservation fluid.
abstract: Corneal transplants have one of the highest success rates among all transplantological procedures. Corneas intended for transplantation are stored in a preservation fluid, which is then tested for bacterial and fungal infections. Among all analyses of infectious complications following corneal transplants, infections caused by bacteria or fungi are the most prominent. Surprisingly, however, apart from a few publications, there is a lack of data regarding the occurrence of viruses in donor corneas and the risk of transmitting these to their recipients. The intention of this research was therefore to determine the frequency with which human herpesvirus 1 (HHV-1), human herpesvirus 2 (HHV-2), and human adenovirus (HAdV) occur in transplanted corneal tissue, as well as in samples of preservation fluid. The study comprised 57 paired samples, with each pair consisting of a fragment of the corneal tissue remaining after its trepanation for transplantation surgery and a sample of corneal preservation fluid. Sample pairs were all tested for the presence of the DNA of three viruses (HHV-1, HHV-2, and HAdV) using real time PCR technique. Viral DNA was found in three of the tested corneas-HHV-1 DNA in one paired sample (1.8%) and adenovirus DNA in two single samples (3.5%). We postulate that virological testing of corneas for transplantation should be considered, particularly in the case of donors with increased risk factors for herpesvirus and adenovirus reactivation. J. Med. Virol. 89:732-736, 2017. © 2016 Wiley Periodicals, Inc.
authors: SPKSO Ophthalmic University Hospital, Medical University of Warsaw, Warsaw, Poland.
authors: G
authors: G
authors: Broniek
authors: SPKSO Ophthalmic University Hospital, Medical University of Warsaw, Warsaw, Poland.|Department of Ophthalmology, Medical University of Warsaw, Warsaw, Poland.
authors: E
authors: E
authors: Langwińska-Wośko
authors: SPKSO Ophthalmic University Hospital, Medical University of Warsaw, Warsaw, Poland.
authors: M
authors: M
authors: Sybilska
authors: SPKSO Ophthalmic University Hospital, Medical University of Warsaw, Warsaw, Poland.|Department of Ophthalmology, Medical University of Warsaw, Warsaw, Poland.
authors: J P
authors: JP
authors: Szaflik
authors: Chair and Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland.
authors: M
authors: M
authors: Przybylski
authors: SPKSO Ophthalmic University Hospital, Medical University of Warsaw, Warsaw, Poland.|Department of Dental Microbiology, Medical University of Warsaw, Warsaw, Poland.
authors: M
authors: M
authors: Wróblewska
mesh_terms: D000260:Adenoviruses, Human / Q000302:isolation & purification*; D000328:Adult; D000369:Aged, 80 and over; D003315:Cornea / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D018259:Herpesvirus 1, Human / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011309:Preservation, Biological / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral
keywords: adenovirus; herpes simplex virus; horizontal transmission