title: Early changes in the knee of healer and non-healer mice following non-invasive mechanical injury.
abstract: In this study, we examined early time-dependent changes in articular cartilage and synovium in response to tibial compression and sought the plausible origin of cells that respond to compression in the healer (LGXSM-6) and non-healer (LGXSM-33) recombinant inbred mouse strains. The right knee of 13-week old male mice was subjected to tibial compression using 9N axial loading. The contralateral left knee served as a control. Knees were harvested at 5, 9, and 14 days post-injury. Histological changes in cartilage and synovium, immunofluorescence pattern of CD44, aggrecan, type-II collagen, cartilage oligomeric matrix protein and the aggrecan neo-epitope NITEGE, and cell apoptosis (by TUNEL) were examined. We used a double nucleoside analog cell-labeling strategy to trace cells responsive to injury. We showed that tibial compression resulted in rupture of anterior cruciate ligament, cartilage matrix loss and chondrocyte apoptosis at the injury site. LGXSM-33 showed higher synovitis and ectopic synovial chondrogenesis than LGXSM-6 with no differences for articular cartilage lesions. With loading, an altered pattern of CD44 and NITEGE was observed: cells in the impacted area underwent apoptosis, cells closely surrounding the injured area expressed CD44, and cells in the intact area expressed NITEGE. Cells responding to injury were found in the synovium, subchondral bone marrow and the Groove of Ranvier. Taken together, we found no strain differences in chondrocytes in the early response to injury. However, the synovial response was greater in LGXSM-33 indicating that, at early time points, there is a genetic difference in synovial cell reaction to injury. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:524-536, 2017.
authors: Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington University School of Medicine at Barnes-Jewish Hospital, 425 S. Euclid Ave. MS 8233, St. Louis, Missouri 63110.|First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors: Xin
authors: X
authors: Duan
authors: Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington University School of Medicine at Barnes-Jewish Hospital, 425 S. Euclid Ave. MS 8233, St. Louis, Missouri 63110.
authors: Muhammad Farooq
authors: MF
authors: Rai
authors: Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington University School of Medicine at Barnes-Jewish Hospital, 425 S. Euclid Ave. MS 8233, St. Louis, Missouri 63110.|Department of Biomedical Engineering, Washington University in St. Louis at Engineering and Applied Sciences, Whitaker Hall, MS 1097, St. Louis, Missouri 63130.
authors: Nilsson
authors: N
authors: Holguin
authors: Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington University School of Medicine at Barnes-Jewish Hospital, 425 S. Euclid Ave. MS 8233, St. Louis, Missouri 63110.|Department of Biomedical Engineering, Washington University in St. Louis at Engineering and Applied Sciences, Whitaker Hall, MS 1097, St. Louis, Missouri 63130.
authors: Matthew J
authors: MJ
authors: Silva
authors: Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington University School of Medicine at Barnes-Jewish Hospital, 425 S. Euclid Ave. MS 8233, St. Louis, Missouri 63110.
authors: Debabrata
authors: D
authors: Patra
authors: First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors: Weiming
authors: W
authors: Liao
authors: Department of Orthopaedic Surgery, Musculoskeletal Research Center, Washington University School of Medicine at Barnes-Jewish Hospital, 425 S. Euclid Ave. MS 8233, St. Louis, Missouri 63110.|Department of Biomedical Engineering, Washington University in St. Louis at Engineering and Applied Sciences, Whitaker Hall, MS 1097, St. Louis, Missouri 63130.|Department of Cell Biology and Physiology, Washington University School of Medicine at Barnes-Jewish Hospital, 425 S. Euclid Ave. MS 8233, St. Louis, Missouri 63110.
authors: Linda J
authors: LJ
authors: Sandell
mesh_terms: D053674:Aggrecans / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D002358:Cartilage, Articular / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D018960:Hyaluronan Receptors / Q000378:metabolism; D007718:Knee Injuries / Q000150:complications* / Q000473:pathology; D008297:Male; D051379:Mice; D020370:Osteoarthritis, Knee / Q000235:genetics*; D013583:Synovial Membrane / Q000473:pathology; D013585:Synovitis / Q000235:genetics* / Q000473:pathology
chemical_list: D053674:Aggrecans; C476399:Cd44 protein, mouse; D016326:Extracellular Matrix Proteins; D018960:Hyaluronan Receptors
keywords: cell source; chondrocyte apoptosis; genetics; knee joint; mechanical injury