title: Function of RSKS-1-AAK-2-DAF-16 signaling cascade in enhancing toxicity of multi-walled carbon nanotubes can be suppressed by mir-259 activation in Caenorhabditis elegans.
abstract: Caenorhabditis elegans is an important non-mammalian alternative assay model for toxicological study. Previous study has indicated that exposure to multi-walled carbon nanotubes (MWCNTs) dysregulated the transcriptional expression of mir-259. In this study, we examined the molecular basis for mir-259 in regulating MWCNTs toxicity in nematodes. Mutation of mir-259 induced a susceptible property to MWCNTs toxicity, and MWCNTs exposure induced a significant increase in mir-259::GFP in pharyngeal/intestinal valve and reproductive tract, implying that mir-259 might mediate a protection mechanisms for nematodes against MWCNTs toxicity. RSKS-1, a putative ribosomal protein S6 kinase, acted as the target for mir-259 in regulating MWCNTs toxicity, and mutation of rsks-1 suppressed the susceptible property of mir-259 mutant to MWCNTs toxicity. Moreover, mir-259 functioned in pharynx-intestinal valve and RSKS-1 functioned in pharynx to regulate MWCNTs toxicity. Furthermore, RSKS-1 regulated MWCNTs toxicity by suppressing the function of AAK-2-DAF-16 signaling cascade. Our results will strengthen our understanding the microRNAs mediated protection mechanisms for animals against the toxicity from certain nanomaterials.
authors: School of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou 213164, China.|Changzhou No. 7 People's Hospital, Changzhou 213011, China.
authors: Ziheng
authors: Z
authors: Zhuang
authors: School of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou 213164, China.|Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast University, Nanjing 210009, China.
authors: Min
authors: M
authors: Li
authors: School of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou 213164, China.
authors: Hui
authors: H
authors: Liu
authors: Changzhou No. 7 People's Hospital, Changzhou 213011, China.
authors: Libo
authors: L
authors: Luo
authors: Changzhou No. 7 People's Hospital, Changzhou 213011, China.
authors: Weidong
authors: W
authors: Gu
authors: Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast University, Nanjing 210009, China.
authors: Qiuli
authors: Q
authors: Wu
authors: Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast University, Nanjing 210009, China.
authors: Dayong
authors: D
authors: Wang
mesh_terms: D055372:AMP-Activated Protein Kinases; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D051858:Forkhead Transcription Factors / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D007422:Intestines / Q000187:drug effects; D035683:MicroRNAs / Q000235:genetics*; D037742:Nanotubes, Carbon / Q000009:adverse effects* / Q000737:chemistry; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017382:Reactive Oxygen Species / Q000378:metabolism; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D029742:Caenorhabditis elegans Proteins; D051858:Forkhead Transcription Factors; C000627196:MIRN-259 microRNA, C elegans; D035683:MicroRNAs; D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species; C108831:daf-16 protein, C elegans; D017346:Protein Serine-Threonine Kinases; D038762:Ribosomal Protein S6 Kinases, 70-kDa; C571892:Rsks-1 protein, C elegans; C510541:AAK-2 protein, C elegans; D055372:AMP-Activated Protein Kinases