title: Mogrol Derived from Siraitia grosvenorii Mogrosides Suppresses 3T3-L1 Adipocyte Differentiation by Reducing cAMP-Response Element-Binding Protein Phosphorylation and Increasing AMP-Activated Protein Kinase Phosphorylation.
abstract: This study investigated the effects of mogrol, an aglycone of mogrosides from Siraitia grosvenorii, on adipogenesis in 3T3-L1 preadipocytes. Mogrol, but not mogrosides, suppressed triglyceride accumulation by affecting early (days 0-2) and late (days 4-8), but not middle (days 2-4), differentiation stages. At the late stage, mogrol increased AMP-activated protein kinase (AMPK) phosphorylation and reduced glycerol-3-phosphate dehydrogenase activity. At the early stage, mogrol promoted AMPK phosphorylation, inhibited the induction of CCAAT/enhancer-binding protein β (C/EBPβ; a master regulator of adipogenesis), and reduced 3T3-L1 cell contents (e.g., clonal expansion). In addition, mogrol, but not the AMPK activator AICAR, suppressed the phosphorylation and activity of the cAMP response element-binding protein (CREB), which regulates C/EBPβ expression. These results indicated that mogrol suppressed adipogenesis by reducing CREB activation in the initial stage of cell differentiation and by activating AMPK signaling in both the early and late stages of this process.
authors: Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.
authors: Naoki
authors: N
authors: Harada
authors: Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.
authors: Mikako
authors: M
authors: Ishihara
authors: Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.
authors: Hiroko
authors: H
authors: Horiuchi
authors: Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.|Biochemical Laboratory, Saraya Company, Ltd., Kashiwara, Osaka, Japan.
authors: Yuta
authors: Y
authors: Ito
authors: Biochemical Laboratory, Saraya Company, Ltd., Kashiwara, Osaka, Japan.
authors: Hiromitsu
authors: H
authors: Tabata
authors: Biochemical Laboratory, Saraya Company, Ltd., Kashiwara, Osaka, Japan.
authors: Yasushi A
authors: YA
authors: Suzuki
authors: Center for Research and Development of Bioresources, Osaka Prefecture University, Sakai, Osaka, Japan.
authors: Yoshihisa
authors: Y
authors: Nakano
authors: Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.
authors: Ryoichi
authors: R
authors: Yamaji
authors: Center for Research and Development of Bioresources, Osaka Prefecture University, Sakai, Osaka, Japan.|Department of Nutrition, Osaka Prefecture University, Habikino, Osaka, Japan.
authors: Hiroshi
authors: H
authors: Inui
mesh_terms: D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D019662:Cucurbitaceae / Q000737:chemistry*; D017362:Cyclic AMP Response Element-Binding Protein / Q000037:antagonists & inhibitors* / Q000378:metabolism; D050356:Lipid Metabolism; D051379:Mice; D010766:Phosphorylation; D015398:Signal Transduction; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D014315:Triterpenes; D055372:AMP-Activated Protein Kinases