title: Synbiotics suppress the release of lactate dehydrogenase, promote non-specific immunity and integrity of jejunum mucosa in piglets.
abstract: The aim of our experiment was to study how synbiotics are able to deal with the problems of post-weaning piglets. Lactobacillus plantarum - Biocenol(TM) LP96 (CCM 7512), Lactobacillus fermentum - Biocenol(TM) LF99 (CCM 7514) and flaxseed (rich in n-3 polyunsaturated fatty acids) were administered to 36 conventional piglets from a problematic breed with confirmed presence of enterotoxigenic Escherichia coli and Coronavirus. The experimental piglets were supplied with probiotic cheeses and crushed flax-seed in the period starting 10 days before weaning and lasting up to 14 days post-weaning. Piglets in the control group were supplied only control cheese. The impact of such additives on the release of lactate dehydrogenase (LDH; spectroscopic and electrophoretic assay), alteration of immunity (index of metabolic activity), jejunum histology (light microscopy), and health of conventional piglets from a problematic breed (monitoring of hematology, consistency and moisture of feces and body temperature) were examined. We found significant decrease in LDH leakage in the blood serum and tissue extracts, indicating better cell membrane integrity in the individual organs of animals. Probiotics and flaxseed applied together seem to be a good source of nutrients to improve the immune status and the integrity of jejunum mucosa during infection. © 2015 Japanese Society of Animal Science.
authors: Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic.
authors: Zuzana
authors: Z
authors: Andrejčáková
authors: Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic.
authors: Drahomíra
authors: D
authors: Sopková
authors: Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic.
authors: Radoslava
authors: R
authors: Vlčková
authors: Department of Epizootology and Preventive Veterinary Medicine, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic.
authors: Lucia
authors: L
authors: Kulichová
authors: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic.
authors: Soňa
authors: S
authors: Gancarčíková
authors: Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic.
authors: Viera
authors: V
authors: Almášiová
authors: Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic.
authors: Katarína
authors: K
authors: Holovská
authors: Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic.
authors: Vladimír
authors: V
authors: Petrilla
authors: Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak Republic.
authors: Lenka
authors: L
authors: Krešáková
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena / Q000502:physiology; D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D017934:Coronavirus; D004926:Escherichia coli; D019597:Flax; D007113:Immunity, Innate; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000276:immunology* / Q000648:ultrastructure; D007583:Jejunum / Q000201:enzymology* / Q000276:immunology* / Q000648:ultrastructure; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D052197:Limosilactobacillus fermentum; D048191:Lactiplantibacillus plantarum; D046529:Microscopy, Electron, Transmission; D013552:Swine / Q000276:immunology* / Q000382:microbiology*; D058616:Synbiotics / Q000008:administration & dosage*; D014886:Weaning
chemical_list: D007770:L-Lactate Dehydrogenase
keywords: gut histology; lactate dehydrogenase; non-specific immunity; piglet; synbiotics