title: The therapeutic potential of sulforaphane on light-induced photoreceptor degeneration through antiapoptosis and antioxidant protection.
abstract: Oxidative stress due to excessive light exposure can exacerbate a variety of human retinal diseases by accelerating photoreceptor cell death. The thioredoxin (Trx) system is considered to play a crucial role in reduction/oxidation (redox) regulation of signal transduction and in cell defense against oxidative stresses. Sulforaphane (SF) protects cells from oxidative damage through nuclear factor (erythroid-derived 2)-like 2 (Nrf2), which is responsible for multiple detoxification processes, including elevating the expression of Trx. This study sought to demonstrate whether SF increased Trx expression in retinal tissues in vivo and whether it could preserve the photoreceptors from degeneration induced by oxidative stress. Our data clearly showed that pretreatment with SF abated photoreceptor cell loss, in association with increased expression of Nrf2 and Trx, subsequently activating the Ras/Raf1/Erk signaling pathway and decreasing the expression of Bak1, Cyt-c release and the activity of caspase-3 in light-induced mouse retinas. These data suggested that the therapeutic potential of SF in retinal degeneration due to oxidative stress might partially involve anti-caspase and antioxidant protection mediated by Trx.
authors: Department of Histoembryology of Dalian Medical University, 116044, Dalian, Liaoning, China.
authors: Li
authors: L
authors: Kong
authors: Department of Histoembryology of Dalian Medical University, 116044, Dalian, Liaoning, China.
authors: Bo
authors: B
authors: Liu
authors: Department of Histoembryology of Dalian Medical University, 116044, Dalian, Liaoning, China.
authors: Chenghong
authors: C
authors: Zhang
authors: Department of Histoembryology of Dalian Medical University, 116044, Dalian, Liaoning, China.
authors: Bing
authors: B
authors: Wang
authors: Department of Histoembryology of Dalian Medical University, 116044, Dalian, Liaoning, China.
authors: Hongfei
authors: H
authors: Wang
authors: Department of Histoembryology of Dalian Medical University, 116044, Dalian, Liaoning, China.
authors: Xiaoxia
authors: X
authors: Song
authors: Department of Histoembryology of Dalian Medical University, 116044, Dalian, Liaoning, China.
authors: Yang
authors: Y
authors: Yang
authors: Department of Histoembryology of Dalian Medical University, 116044, Dalian, Liaoning, China.
authors: Xiang
authors: X
authors: Ren
authors: Department of Oncology, Dalian Central Hospital, 116033, Dalian, Liaoning, China.
authors: Liangwei
authors: L
authors: Yin
authors: Department of Otorhinolaryngology, The Second Hospital of Dalian Medical University, 116023, Dalian, Liaoning, China. Electronic address: konghui6905@163.com.
authors: Hui
authors: H
authors: Kong
authors: Department of Histoembryology of Dalian Medical University, 116044, Dalian, Liaoning, China. Electronic address: hyma20060602@aliyun.com.
authors: Haiying
authors: H
authors: Ma
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D017879:Isothiocyanates / Q000494:pharmacology*; D008027:Light; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects; D012162:Retinal Degeneration / Q000139:chemically induced / Q000188:drug therapy; D013454:Sulfoxides; D013879:Thioredoxins / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017879:Isothiocyanates; D013454:Sulfoxides; D013879:Thioredoxins; C016766:sulforaphane
keywords: Apoptosis; Oxidative stress; Retinal degeneration; Sulforaphane; Thioredoxin