title: Effects of opioids on proximal renal tubular cells undergoing ATP depletion.
abstract: This study investigated the effect of morphine, fentanyl, butorphanol and buprenorphine on viability and caspase-3 activity in renal proximal tubular cells exposed to opioids for 2 h before or 12 h after chemical anoxia. Cell viability decreased regardless the treatment although intracellular ATP content was elevated in morphine and fentanyl pre-treated cells at 12 h. Anoxia increased caspase activity but this effect was significantly reduced in cells treated before or after with morphine, fentanyl and in cell treated with butorphanol for 12 h. No influence of buprenorphine was detected. Morphine, fentanyl and butorphanol might have protective effects during kidney ischemia.
authors: Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università 16, 30128, Padua, Legnaro, Italy. Electronic address: luca.bellini@unipd.it.
authors: Luca
authors: L
authors: Bellini
authors: CORIT (Consortium for Research in Organ Transplantation), Via Giustiniani 2, 35128, Padua, Italy.
authors: Marta
authors: M
authors: Vadori
authors: Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università 16, 30128, Padua, Legnaro, Italy; CORIT (Consortium for Research in Organ Transplantation), Via Giustiniani 2, 35128, Padua, Italy.
authors: Giulia Maria
authors: GM
authors: De Benedictis
authors: Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università 16, 30128, Padua, Legnaro, Italy.
authors: Roberto
authors: R
authors: Busetto
mesh_terms: D000255:Adenosine Triphosphate / Q000172:deficiency / Q000378:metabolism*; D000701:Analgesics, Opioid / Q000494:pharmacology*; D000818:Animals; D000968:Antimycin A; D002047:Buprenorphine / Q000494:pharmacology*; D002077:Butorphanol / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D015687:Cell Hypoxia / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003847:Deoxyglucose; D005283:Fentanyl / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism; D009020:Morphine / Q000494:pharmacology*; D009893:Opossums
chemical_list: D000701:Analgesics, Opioid; D002047:Buprenorphine; D000968:Antimycin A; D009020:Morphine; D000255:Adenosine Triphosphate; D003847:Deoxyglucose; D053148:Caspase 3; D002077:Butorphanol; D005283:Fentanyl
keywords: Anoxia; Opioids; Tubular kidney cells