title: Delignification outperforms alkaline extraction for xylan fingerprinting of oil palm empty fruit bunch.
abstract: Enzyme hydrolysed (hemi-)celluloses from oil palm empty fruit bunches (EFBs) are a source for production of bio-fuels or chemicals. In this study, after either peracetic acid delignification or alkaline extraction, EFB hemicellulose structures were described, aided by xylanase hydrolysis. Delignification of EFB facilitated the hydrolysis of EFB-xylan by a pure endo-β-1,4-xylanase. Up to 91% (w/w) of the non-extracted xylan in the delignified EFB was hydrolysed compared to less than 4% (w/w) of that in untreated EFB. Alkaline extraction of EFB, without prior delignification, yielded only 50% of the xylan. The xylan obtained was hydrolysed only for 40% by the endo-xylanase used. Hence, delignification alone outperformed alkaline extraction as pretreatment for enzymatic fingerprinting of EFB xylans. From the analysis of the oligosaccharide-fingerprint of the delignified endo-xylanase hydrolysed EFB xylan, the structure was proposed as acetylated 4-O-methylglucuronoarabinoxylan.
authors: Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.
authors: Patricia
authors: P
authors: Murciano Martínez
authors: Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands. Electronic address: mirjam.kabel@wur.nl.
authors: Mirjam A
authors: MA
authors: Kabel
authors: Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.
authors: Harry
authors: H
authors: Gruppen
mesh_terms: D028023:Arecaceae / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D008672:Metals, Alkali / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D010463:Peracetic Acid / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D014990:Xylans / Q000737:chemistry*
chemical_list: D008672:Metals, Alkali; D009844:Oligosaccharides; D011134:Polysaccharides; D014990:Xylans; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D043364:Endo-1,4-beta Xylanases; D010463:Peracetic Acid
keywords: Delignification; Enzymes; Oil palm; Sequential extraction; Xylan