title: Haspin kinase regulates microtubule-organizing center clustering and stability through Aurora kinase C in mouse oocytes.
abstract: Meiotic oocytes lack classic centrosomes and, therefore, bipolar spindle assembly depends on clustering of acentriolar microtubule-organizing centers (MTOCs) into two poles. However, the molecular mechanism regulating MTOC assembly into two poles is not fully understood. The kinase haspin (also known as GSG2) is required to regulate Aurora kinase C (AURKC) localization at chromosomes during meiosis I. Here, we show that inhibition of haspin perturbed MTOC clustering into two poles and the stability of the clustered MTOCs. Furthermore, we show that AURKC localizes to MTOCs in mouse oocytes. Inhibition of haspin perturbed the localization of AURKC at MTOCs, and overexpression of AURKC rescued the MTOC-clustering defects in haspin-inhibited oocytes. Taken together, our data uncover a role for haspin as a regulator of bipolar spindle assembly by regulating AURKC function at acentriolar MTOCs in oocytes.
authors: Department of Genetics, 145 Bevier Road, Rutgers, The State University of New Jersey, Piscataway, NJ 08854-8082, USA Theriogenology Department, Faculty of Veterinary Medicine, Mansoura University, 60 Elgomhoria Street, 35516 Mansoura, Egypt.
authors: Ahmed Z
authors: 0000-0001-9663-6262
authors: AZ
authors: Balboula
authors: Department of Genetics, 145 Bevier Road, Rutgers, The State University of New Jersey, Piscataway, NJ 08854-8082, USA.
authors: Alexandra L
authors: 0000-0001-9920-5900
authors: AL
authors: Nguyen
authors: Department of Genetics, 145 Bevier Road, Rutgers, The State University of New Jersey, Piscataway, NJ 08854-8082, USA.
authors: Amanda S
authors: AS
authors: Gentilello
authors: Department of Genetics, 145 Bevier Road, Rutgers, The State University of New Jersey, Piscataway, NJ 08854-8082, USA.
authors: Suzanne M
authors: SM
authors: Quartuccio
authors: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Rumburská 89, 277 21, Liběchov, Czech Republic.
authors: David
authors: D
authors: Drutovic
authors: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Rumburská 89, 277 21, Liběchov, Czech Republic.
authors: Petr
authors: P
authors: Solc
authors: Department of Genetics, 145 Bevier Road, Rutgers, The State University of New Jersey, Piscataway, NJ 08854-8082, USA schindler@biology.rutgers.edu.
authors: Karen
authors: 0000-0001-6113-1184
authors: K
authors: Schindler
mesh_terms: D000818:Animals; D064108:Aurora Kinase C / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008677:Metaphase; D051379:Mice; D022101:Microtubule-Organizing Center / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D021381:Protein Transport; D008941:Spindle Apparatus / Q000378:metabolism
chemical_list: C530657:Haspin protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D064108:Aurora Kinase C; D017346:Protein Serine-Threonine Kinases
keywords: Aurora kinase; Haspin; MTOC; Oocyte; Spindle