title: Effect of alpha-lipoic acid on relieving ammonia stress and hepatic proteomic analyses of broilers.
abstract: Ammonia in poultry houses not only affects worker health but also induces a variety of poultry diseases. Alpha-lipoic acid (LA) is an effective antioxidant that protects cells against oxidative injury during various toxic and pathological processes. This study was designed to evaluate the mitigating effects of LA supplementation on ammonia stress and hepatic proteome changes in broilers. Male broilers (22 d old) were allocated to 3 groups: (1) a control group without ammonia stress (CTRL); (2) exposure to 70 ppm ammonia (AM); and (3) exposure to 70 ppm ammonia and dietary administration of 300 mg/kg LA (AM+LA). Ammonia exposure significantly decreased broiler growth performance and plasma glutathione peroxidase activity (P < 0.05), and increased plasma malondialdehyde content and glutamic-pyruvic transaminase activity (P < 0.05). These negative effects were eliminated by LA supplementation. Comparative proteomic analyses revealed 291 differentially expressed proteins in the AM group compared to the CTRL and AM+LA groups. A total of 30 proteins were differentially expressed between the AM/CTRL and (AM+LA)/AM groups. The addition of LA restored 24 of these proteins to control levels; these proteins were mainly related to transcription regulation, detoxification, protein translation and degradation, and immune and stress responses. The differentially expressed proteins included the high mobility group box (HMGB) and glutathione S-transferase (GST), which is closely related to immune response and oxidative stress, and collagens, which are implicated in liver injury. The addition of LA to broiler diet may reduce ammonia toxicity by maintaining the antioxidant system, xenobiotic metabolism, and metabolic pathways.
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China, 450002.|Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China, 450002.
authors: M
authors: M
authors: Lu
authors: Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China, 450002.
authors: J
authors: J
authors: Bai
authors: Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China, 450002.
authors: B
authors: B
authors: Xu
authors: Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China, 450002.
authors: Q Y
authors: QY
authors: Sun
authors: Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China, 450002.
authors: F X
authors: FX
authors: Wei
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, The Chinese Academy of Agricultural Sciences, Beijing, China, 100093.
authors: X F
authors: XF
authors: Tang
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, The Chinese Academy of Agricultural Sciences, Beijing, China, 100093.
authors: H F
authors: HF
authors: Zhang
authors: Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China, 450002.
authors: J
authors: J
authors: Li
authors: Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China, 450002.
authors: G L
authors: GL
authors: Wang
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China, 450002 qqy1964@126.com lsy9617@aliyun.com.
authors: Q Q
authors: QQ
authors: Yin
authors: Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China, 450002 qqy1964@126.com lsy9617@aliyun.com.
authors: S Y
authors: SY
authors: Li
mesh_terms: D000641:Ammonia / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D030161:Avian Proteins / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000662:veterinary; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D020543:Proteome; D011897:Random Allocation; D053719:Tandem Mass Spectrometry / Q000662:veterinary; D008063:Thioctic Acid / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D030161:Avian Proteins; D020543:Proteome; D008063:Thioctic Acid; D000641:Ammonia
keywords: alpha-lipoic acid; ammonia stress; broiler; production performance; proteomics