title: Increased angiogenic factors in exhaled breath condensate of children with severe asthma - New markers of disease progression?
abstract: Asthma progression is associated with airway remodeling and neo-vascularization. However, role of angiogenesis in these changes remains unclear and available data still incomplete. In this pilot study we verify usefulness of proteome profiler assay in screening of angiogenesis-related factors in exhaled breath condensates (EBC) collected from children with asthma. EBC samples from patients with mild or severe asthma and healthy controls were tested using protein array. In EBC samples from patients with severe asthma we have found large quantities of several angiogenesis regulators, including thrombospondin (TSP)-1, angiogenin, dipeptidyl peptidase (DPP) IV, matrix metalloproteinase (MMP)-9 and its inhibitor TIMP-1. Small amounts of angiopoietin (Ang)-2 and vascular endothelial growth factor (VEGF) were also present. In contrast to them, in EBC from mild asthma group we have detected TSP-1 and small quantities of Ang-2. EBC samples from healthy controls contained only TSP-1. Our preliminary report suggests that, since increased amounts of angiogenesis-related factors in EBC seem to correlate with asthma severity, they may be considered as convenient non-invasive markers of disease progression. However, further research is necessary.
authors: Department of Paediatrics, Pneumonology and Allergology, Medical University of Warsaw, Poland.
authors: Katarzyna
authors: K
authors: Grzela
authors: Department of Histology and Embryology, Medical University of Warsaw, Poland; Potgraduate School of Molecular Medicine, Medical University of Warsaw, Poland.
authors: Malgorzata
authors: M
authors: Litwiniuk
authors: Department of Histology and Embryology, Medical University of Warsaw, Poland.
authors: Alicja
authors: A
authors: Krejner
authors: Department of Paediatrics, Pneumonology and Allergology, Medical University of Warsaw, Poland.
authors: Wioletta
authors: W
authors: Zagorska
authors: Department of Histology and Embryology, Medical University of Warsaw, Poland. Electronic address: tomekgrzela@gmail.com.
authors: Tomasz
authors: T
authors: Grzela
mesh_terms: D000293:Adolescent; D056151:Airway Remodeling; D043925:Angiogenesis Inducing Agents / Q000378:metabolism*; D001249:Asthma / Q000175:diagnosis* / Q000378:metabolism / Q000503:physiopathology; D015415:Biomarkers / Q000378:metabolism; D001944:Breath Tests / Q000379:methods*; D002648:Child; D018450:Disease Progression; D045853:Exhalation; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D020543:Proteome; D012720:Severity of Illness Index; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D043925:Angiogenesis Inducing Agents; D015415:Biomarkers; D020543:Proteome; D019715:Tissue Inhibitor of Metalloproteinase-1; D042461:Vascular Endothelial Growth Factor A; D020780:Matrix Metalloproteinase 9
keywords: Angiogenesis; Asthma; Exhaled breath condensate; Proteome profiler; Remodeling