title: Morphological Analysis of Cell Death by Cytospinning Followed by Rapid Staining.
abstract: Identifying and characterizing different forms of cell death can be facilitated by staining internal cellular structures with dyes such as hematoxylin and eosin (H&E). These dyes stain the nucleus and cytoplasm, respectively, and optimized reagents (e.g., Rapi-Diff, Rapid Stain, or Quick Dip) are commonly used in pathology laboratories. Fixing and staining adherent cells with these optimized reagents is a straightforward procedure, but apoptotic cells may detach from the culture plate and be washed away during the fixing and staining procedure. To prevent the loss of apoptotic cells, cells can be gently centrifuged onto glass slides by cytospinning before fixing and staining. In addition to apoptotic cells, this procedure can be used on cells in suspension, or adherent cells that have been trypsinized and removed from the culture dish. This protocol describes cytospinning followed by Rapi-Diff staining for morphological analysis of cell death.
authors: Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia;
authors: Lisa C
authors: LC
authors: Crowley
authors: Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia;
authors: Brooke J
authors: BJ
authors: Marfell
authors: Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia; Flow Cytometry and Imaging, QIMR Berghofer Medical Research Institute, Herston, Brisbane, Queensland 4006, Australia; School of Medicine, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.
authors: Nigel J
authors: NJ
authors: Waterhouse
mesh_terms: D016923:Cell Death; D002498:Centrifugation / Q000379:methods*; D003584:Cytological Techniques / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors