title: Anesthesia by sprinkling method in the gills of tambaqui Colossoma macropomum does not influence intensity and morphology of monogeneans.
abstract: The present study evaluates the influence of anesthesia on the parasitic fauna of monogenea fish parasites, as its intensity and viability. Two experiments were conducted: Evaluation of an anesthetic method by sprinkling eugenol directly on gills and evaluation of monogenea motility and viability; Comparison of immersion and directly sprinkling on the gills with benzocaine and eugenol followed by evaluation on parasite intensity. The results suggest that the anesthetic sprinkling didn't interfere in the parasite motility, morphology and body surface integrity analyzed by fluorescence method. The monogenean intensity in the gills was lower in fish anesthetized by immersion method compared to the sprinkling method and the control group. This method of anesthesia can be used in parasitological studies.
authors: Embrapa Amazonia Ocidental, Rod. AM 010, Km 29, CEP 69010-970, Manaus, AM, Brazil.
authors: C L
authors: CL
authors: Boijink
authors: Embrapa Pesca e Aquicultura, Quadra 104 Sul, Avenida LO 1, 34, Conjunto 4, CEP 77020-020, Palmas TO, Brazil.
authors: P O
authors: PO
authors: Maciel
authors: Embrapa Amapá, Rod. Juscelino Kubitscheck, 2600, Km 5, CP 10, CEP 68906-970, Macapá, AP, Brazil.
authors: M
authors: M
authors: Tavares-Dias
authors: Embrapa Pesca e Aquicultura, Quadra 104 Sul, Avenida LO 1, 34, Conjunto 4, CEP 77020-020, Palmas TO, Brazil.
authors: M K P
authors: MKP
authors: Iwashita
authors: Uninorte Manaus, Av. Leonardo Malcher, 853, Centro, CEP 69010-060, Manaus, AM, Brazil.
authors: M S
authors: MS
authors: Morais
authors: Universidade Tiradentes - UNIT, Av. Murilo Dantas, 300, Farolândia, CEP 49032-490, Aracaju, SE, Brazil.
authors: D M V
authors: DMV
authors: Hide
authors: Universidade Federal do Pará - UFPA, Rua Augusto Corrêa, Guamá, CP 479, CEP 66075-110, Belém, PA, Brazil.
authors: N C
authors: NC
authors: Souza
authors: Universidade Federal do Pará - UFPA, Rua Augusto Corrêa, Guamá, CP 479, CEP 66075-110, Belém, PA, Brazil.
authors: M V S
authors: MVS
authors: Couto
authors: Universidade Tiradentes - UNIT, Av. Murilo Dantas, 300, Farolândia, CEP 49032-490, Aracaju, SE, Brazil.
authors: J O
authors: JO
authors: Meneses
authors: Universidade Tiradentes - UNIT, Av. Murilo Dantas, 300, Farolândia, CEP 49032-490, Aracaju, SE, Brazil.
authors: F S
authors: FS
authors: Cunha
authors: Embrapa Tabuleiros Costeiros, Avenida Beira Mar, 3250, CEP 49025-040, Aracaju, SE, Brazil.
authors: R Y
authors: RY
authors: Fujimoto
mesh_terms: D000758:Anesthesia / Q000662:veterinary; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D001566:Benzocaine / Q000494:pharmacology*; D060125:Characiformes / Q000469:parasitology* / Q000502:physiology*; D005054:Eugenol / Q000494:pharmacology*; D005880:Gills / Q000187:drug effects / Q000469:parasitology / Q000502:physiology; D010986:Platyhelminths / Q000187:drug effects*
chemical_list: D000777:Anesthetics; D005054:Eugenol; D001566:Benzocaine