title: Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness.
abstract: BACKGROUND
  Coxsackievirus B3 (CVB3) virus has been implicated as the causative agent of various outbreaks of clinical disease, including hand, foot, and mouth diseases, aseptic meningitis, acute myocarditis, and inflammatory cardiomyopathy.
  
  
  METHODS
  Two hundred and nine undiagnosed cryopreserved specimens obtained from factory workers in Bandung, Indonesia, who displayed symptoms of acute febrile illness were gathered. Total RNA was isolated from serum and tested by conventional polymerase chain reaction (PCR) using Enterovirus genus-level primers and confirmed by sequencing. Concurrently, the virus was isolated in LLC-MK2 cells.
  
  
  RESULTS
  CVB3 virus was identified in an archived specimen from a patient who presented with symptoms of fever, headache, myalgia, and nausea. Sequencing results of the VP1 region from both the clinical sample and tissue culture supernatant showed 97% homology to a CVB3 virus isolate from Taiwan. Virus propagation in LLC-MK2 cell culture exhibited severe cytopathic effects two days post-inoculation.
  
  
  CONCLUSIONS
  We report the first case of CVB3 from an undifferentiated febrile illness specimen from Indonesia.
authors: Eijkman Institute for Molecular Biology, Jakarta, Indonesia. ageng@eijkman.go.id.
authors: Ageng
authors: A
authors: Wiyatno
authors: Ungke
authors: U
authors: Antonjaya
authors: Chairin Nisa
authors: CN
authors: Ma'roef
authors: Silvita Fitri
authors: SF
authors: Riswari
authors: Hofiya
authors: H
authors: Djauhari
authors: I Made
authors: IM
authors: Artika
authors: Corina
authors: C
authors: Monagin
authors: Bradley S
authors: BS
authors: Schneider
authors: Khin Saw
authors: KS
authors: Myint
authors: Bachti
authors: B
authors: Alisjahbana
authors: Dodi
authors: D
authors: Safari
authors: Herman
authors: H
authors: Kosasih
mesh_terms: D000328:Adult; D015331:Cohort Studies; D029822:Enterovirus B, Human / Q000145:classification* / Q000302:isolation & purification*; D005260:Female; D005335:Fever of Unknown Origin / Q000175:diagnosis*; D006801:Humans; D007214:Indonesia; D008297:Male; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D044967:Serum / Q000821:virology*; D014776:Virus Cultivation
chemical_list: D012367:RNA, Viral