title: Pancreatic Islets: Methods for Isolation and Purification of Juvenile and Adult Pig Islets.
abstract: The current situation of organ transplantation is mainly determined by the disbalance between the number of available organs and the number of patients on the waiting list. This obvious dilemma might be solved by the transplantation of porcine organs into human patients. The metabolic similarities which exist between both species made pancreatic islets of Langerhans to that donor tissue which will be most likely transplanted in human recipients. Nevertheless, the successful isolation of significant yields of viable porcine islets is extremely difficult and requires extensive experiences in the field. This review is focussing on the technical challenges, pitfalls and particularities that are associated with the isolation of islets from juvenile and adult pigs considering donor variables that can affect porcine islet isolation outcome.
authors: Nuffield Department of Surgical Sciences, University of Oxford, Churchill Hospital, Old Road, Oxford, OX3 7LJ, UK. Heide.Brandhorst@nds.ox.ac.uk.|Oxford Centre for Diabetes, Endocrinology and Metabolism, Oxford, UK. Heide.Brandhorst@nds.ox.ac.uk.
authors: Heide
authors: H
authors: Brandhorst
authors: Nuffield Department of Surgical Sciences, University of Oxford, Churchill Hospital, Old Road, Oxford, OX3 7LJ, UK.|Oxford Centre for Diabetes, Endocrinology and Metabolism, Oxford, UK.|Oxford NIHR Biomedical Research Centre, Oxford, UK.
authors: Paul R V
authors: PR
authors: Johnson
authors: Nuffield Department of Surgical Sciences, University of Oxford, Churchill Hospital, Old Road, Oxford, OX3 7LJ, UK.|Oxford Centre for Diabetes, Endocrinology and Metabolism, Oxford, UK.
authors: Daniel
authors: D
authors: Brandhorst
mesh_terms: D000375:Aging; D000818:Animals; D002499:Centrifugation, Density Gradient; D007515:Islets of Langerhans / Q000502:physiology*; D034421:Sus scrofa; D046509:Tissue Culture Techniques / Q000379:methods*; D009927:Tissue and Organ Procurement
keywords: Islet xenotransplantation; Pig islet isolation; Pig islet purification; Porcine donor variables