title: A Potentiometric Addressable Photoelectrochemical Biosensor for Sensitive Detection of Two Biomarkers.
abstract: It is a great challenge to fabricate multiplex and convenient photoelectrochemical biosensors for ultrasensitive determination of biomarkers. Herein, a fascinating potentiometric addressable photoelectrochemical biosensor was reported for double biomarkers' detection by varying the applied bias in the detection process. In this biosensor, the nanocomposite of cube anatase TiO2mesocrystals and polyamidoamine dendrimers modified a dual disk electrode as an excellent photoelectrochemical sensing matrix. Subsequently, two important biomarkers in serum for prostate cancer, prostate-specific antigen and human interleukin-6, were immobilized onto the different disks of modified electrode via glutaraldehyde bridges. Then another two photosensitizers, graphitic-carbon-nitride-labeled and CS-AgI-labeled different antibodies, were self-assembled onto the electrode surface by a corresponding competitive immune recognition reaction. The change in photocurrent with the target antigen concentration at different critical voltages enables us to selectively and quantitatively determine targets. The results demonstrated that this potentiometric addressable photoelectrochemical biosensing strategy not only has great promise as a new point-of-care diagnostic tool for early detection of prostate cancer but also can be conveniently expanded to multiplex biosensing by simply change biomarkers. More importantly, this work provides an unambiguous operating guideline of multiplex photoelectrochemical immunoassay.
authors: College of Chemistry and Chemical Engineering, Fujian Normal University , Fuzhou 350108, P. R. China.
authors: Hong
authors: H
authors: Dai
authors: College of Chemistry and Chemical Engineering, Fujian Normal University , Fuzhou 350108, P. R. China.
authors: Shupei
authors: S
authors: Zhang
authors: College of Physics and Energy, Fujian Normal University , Fuzhou 350108, P. R. China.
authors: Zhensheng
authors: Z
authors: Hong
authors: College of Chemistry and Chemical Engineering, Fujian Normal University , Fuzhou 350108, P. R. China.
authors: Yanyu
authors: Y
authors: Lin
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D050091:Dendrimers / Q000737:chemistry; D004566:Electrodes; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis*; D007610:Kallikreins / Q000032:analysis*; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D011199:Potentiometry / Q000379:methods*; D017430:Prostate-Specific Antigen / Q000032:analysis*; D014025:Titanium / Q000737:chemistry
chemical_list: D015415:Biomarkers; D050091:Dendrimers; C508600:IL6 protein, human; D015850:Interleukin-6; C531249:Poly(amidoamine); D011073:Polyamines; C009495:titanium dioxide; D014025:Titanium; C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen