title: Downstream extraction process development for recovery of organic acids from a fermentation broth.
abstract: The present study focused on organic acids (OAs) recovery from an acidogenic fermentation broth, which is the main problem regarding the use of OAs for production of ester-based new generation biofuels or other applications. Specifically, 10 solvents were evaluated for OAs recovery from aqueous media and fermentation broths. The effects of pH, solvent/OAs solution ratios and application of successive extractions were studied. The 1:1 solvent/OAs ratio showed the best recovery rates in most cases. Butyric and isobutyric acids showed the highest recovery rates (80-90%), while lactic, succinic, and acetic acids were poorly recovered (up to 45%). The OAs recovery was significantly improved by successive 10-min extractions. Alcohols presented the best extraction performance. The process using repeated extractions with 3-methyl-1-butanol led to the highest OAs recovery. However, 1-butanol can be considered as the most cost-effective option taking into account its price and availability.
authors: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors: Argyro
authors: A
authors: Bekatorou
authors: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors: Agapi
authors: A
authors: Dima
authors: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors: Panagiotia
authors: P
authors: Tsafrakidou
authors: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors: Konstantina
authors: K
authors: Boura
authors: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors: Katerina
authors: K
authors: Lappa
authors: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors: Panagiotis
authors: P
authors: Kandylis
authors: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors: Katerina
authors: K
authors: Pissaridi
authors: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece.
authors: Maria
authors: M
authors: Kanellaki
authors: Food Biotechnology Group, Department of Chemistry, University of Patras, Patras 26500, Greece. Electronic address: a.a.koutinas@upatras.gr.
authors: Athanasios A
authors: AA
authors: Koutinas
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000143:Acids / Q000737:chemistry; D000438:Alcohols / Q000737:chemistry; D056804:Biofuels; D001709:Biotechnology / Q000191:economics / Q000379:methods*; D005591:Chemical Fractionation / Q000379:methods*; D003362:Cost-Benefit Analysis; D005285:Fermentation; D009930:Organic Chemicals / Q000737:chemistry; D000439:Pentanols / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*
chemical_list: D000143:Acids; D000438:Alcohols; D056804:Biofuels; D009930:Organic Chemicals; D000439:Pentanols; D012997:Solvents; D020001:1-Butanol; C029683:isopentyl alcohol
keywords: Acidogenic fermentation; Ester-based biofuels; Organic acids; Solvent extraction