title: Culture time and reagent minimization in the chemical PCC assay.
abstract: The possibility to reduce the culture time and volume of blood and reagents required for the chemical Premature Chromosome Condensation (PCC) assay is demonstrated in this work. Peripheral whole blood was exposed to gamma radiation (1-20 Gy). Lymphocytes were cultured for 40 h, using 50 μl of blood and 450 μl of culture medium. The dose-response curves were adjusted, using length ratio (LR) of the longest to the shortest chromosome piece, and the frequency of rings per cell. No statistical differences were found between the results obtained with this method and those reported with the classical PCC assay culture. The minimization of culture time and reagents in combination with the automatic measurement of the LR of the chromosome pieces, or the scoring of rings, can be a valuable biodosimetry tool in a mass casualty scenario.
authors: a Center of Radiation Protection and Hygiene , Playa , La Habana , Cuba.
authors: Ivonne
authors: I
authors: Romero
authors: a Center of Radiation Protection and Hygiene , Playa , La Habana , Cuba.
authors: Ana Ilsa
authors: AI
authors: Lamadrid
authors: a Center of Radiation Protection and Hygiene , Playa , La Habana , Cuba.
authors: Jorge Ernesto
authors: JE
authors: González
authors: a Center of Radiation Protection and Hygiene , Playa , La Habana , Cuba.
authors: Tania
authors: T
authors: Mandina
authors: a Center of Radiation Protection and Hygiene , Playa , La Habana , Cuba.
authors: Omar
authors: O
authors: García
mesh_terms: D001681:Biological Assay / Q000379:methods*; D002869:Chromosome Aberrations / Q000528:radiation effects*; D003470:Culture Media / Q000737:chemistry; D020732:Cytogenetic Analysis / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D007202:Indicators and Reagents / Q000737:chemistry; D008214:Lymphocytes / Q000528:radiation effects*; D011829:Radiation Dosage; D000069079:Radiation Exposure / Q000032:analysis*; D011834:Radiation Monitoring / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D003470:Culture Media; D007202:Indicators and Reagents
keywords: Blood volume; PCC assay; culture time; cytogenetic; mass casualty scenario; radiological accident