title: FT-IR and FT-Raman characterization of non-cellulosic polysaccharides fractions isolated from plant cell wall.
abstract: The purpose of this work was to reveal the structural changes of cell wall polysaccharides' fractions during tomato fruit development by analysis of spectral data. Mature green and red ripe tomato fruit were taken into consideration. The FT-IR spectra of water soluble pectin (WSP), imidazole soluble pectin (ISP) and diluted alkali soluble pectin (DASP) contained bands typical for pectins. Whereas for KOH fraction spectra bands typical for hemicelluloses were present. The FT-IR spectra showed the drop down of esterification degree of WSP and ISP polysaccharides during maturation. The changes in polysaccharides structure revealed by spectra were the most visible in the case of pectic polysaccharides. The WSP and DASP fraction pectins molecules length were shortened during tomato maturation and ripening. Whereas the ISP fraction spectra analysis showed that this fraction contained rhamnogalacturonan I, but also for red ripe was rich in pectic galactan comparing with ISP fraction from mature green.
authors: Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland. Electronic address: m.chylinska@ipan.lublin.pl.
authors: Monika
authors: M
authors: Chylińska
authors: Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland. Electronic address: m.szymanska@ipan.lublin.pl.
authors: Monika
authors: M
authors: Szymańska-Chargot
authors: Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland. Electronic address: a.zdunek@ipan.lublin.pl.
authors: Artur
authors: A
authors: Zdunek
mesh_terms: D002473:Cell Wall / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry / Q000166:cytology / Q000254:growth & development; D018551:Solanum lycopersicum / Q000166:cytology* / Q000254:growth & development; D010368:Pectins / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D011134:Polysaccharides; D010368:Pectins; C003181:polygalacturonic acid
keywords: Cell wall; FT-IR spectroscopy; FT-Raman spectroscopy; Fruit development; PCA; Polysaccharides