title: Suppressive Effect of Kampo Formula "Juzen-taiho-to" on Carbon Tetrachloride-Induced Hepatotoxicity in Mice.
abstract: The aim of the present study was to investigate whether pretreatment with the Japanese herbal medicine, "Juzen-taiho-to" (JTX), had an ameliorative effect on carbon tetrachloride (CCl4)-induced hepatotoxicity through anorexia prevention. Mice injected with CCl4 exhibited severe anorexia. Moreover, CCl4 increased the plasma levels of hepatic injury markers (i.e., alanine aminotransferase and aspartate aminotransferase), lipid peroxidation, and hepatic Ca(2+) levels. Pretreatment with JTX recovered the CCl4-induced anorexia. In addition, JTX pretreatment decreased CCl4-induced plasma levels of hepatic injury markers. Increased Ca(2+) is a known indicator of the final progression to hepatocyte death, and CCl4-induced hepatotoxicity is mainly caused by oxidative stress. The present study indicated CCl4-induced lipid peroxidation and hepatic Ca(2+) content decreased with JTX pretreatment. Our results suggest that JTX has potential to protect of CCl4-induced anorexia, and the modulation of oxidative stress.
authors: College of Pharmacy, Kinjo Gakuin University.
authors: Hiroki
authors: H
authors: Yoshioka
authors: Shiori
authors: S
authors: Fukaya
authors: Nobuhiko
authors: N
authors: Miura
authors: Satomi
authors: S
authors: Onosaka
authors: Tsunemasa
authors: T
authors: Nonogaki
authors: Akito
authors: A
authors: Nagatsu
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000097:blood; D002118:Calcium / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D020835:Medicine, Kampo; D008668:Metallothionein / Q000378:metabolism; D051379:Mice; D008517:Phytotherapy
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; C063587:juzentaihoto; D008315:Malondialdehyde; D008668:Metallothionein; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D002118:Calcium