title: A polysaccharide from the leaves of Aralia elata induces apoptosis in U-2 OS cells via mitochondrial-dependent pathway.
abstract: In the present study, we isolated and characterized one purified polysaccharide (AEP-1) from the leaves of Aralia elata, and investigated its effect on human osteosarcoma (OS) U-2 OS cell line and analyzed its mechanism. MTT assays showed that AEP-1 markedly inhibited the growth of U-2 OS cells in a dose- and time-dependent fashion, suggesting a cytotoxic effect. The AEP-1 also dose-dependently induced DNA fragmentation and caused apoptotic death in U-2 OS cells. The event of apoptosis was accompanied by increased ratio of Bax/Bcl-2, depolarization of mitochondrial membrane potential (Δym) and the release of cytochrome c from mitochondria into the cytoplasm. Moreover, AEP-1 treatment triggered the activation of caspase-9 and caspase-3, as well as the cleavage of poly (ADP-ribose) polymerase (PAPR) in U-2 OS cells. All these results suggests that the induction of apoptosis by AEP-1 in U-2 OS cells occurs through the mitochondria-dependent pathway and AEP-1 may be useful in treating OS and improving cancer chemotherapy.
authors: Department of Orthopaedic Trauma, The First Bethune Hospital of Jilin University, Changchun, People's Republic of China.
authors: Guangchen
authors: G
authors: Liu
authors: College of Pharmacy, Beihua University, Jilin, People's Republic of China.
authors: Yu
authors: Y
authors: Sheng
authors: College of Chemistry, Jilin University, Changchun, People's Republic of China.
authors: Mei
authors: M
authors: Zhang
authors: Department of Orthopaedic Trauma, The First Bethune Hospital of Jilin University, Changchun, People's Republic of China. Electronic address: sundahui1971@126.com.
authors: Dahui
authors: D
authors: Sun
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D027983:Aralia / Q000737:chemistry*; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism; D012516:Osteosarcoma / Q000188:drug therapy* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D009363:Neoplasm Proteins; D011134:Polysaccharides
keywords: Aralia elata; Human osteosarcoma; Polysaccahride