title: Epithelial Tumors Originate in Tumor Hotspots, a Tissue-Intrinsic Microenvironment.
abstract: Malignant tumors are caused by uncontrolled proliferation of transformed mutant cells that have lost the ability to maintain tissue integrity. Although a number of causative genetic backgrounds for tumor development have been discovered, the initial steps mutant cells take to escape tissue integrity and trigger tumorigenesis remain elusive. Here, we show through analysis of conserved neoplastic tumor-suppressor genes (nTSGs) in Drosophila wing imaginal disc epithelia that tumor initiation depends on tissue-intrinsic local cytoarchitectures, causing tumors to consistently originate in a specific region of the tissue. In this "tumor hotspot" where cells constitute a network of robust structures on their basal side, nTSG-deficient cells delaminate from the apical side of the epithelium and begin tumorigenic overgrowth by exploiting endogenous Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling activity. Conversely, in other regions, the "tumor coldspot" nTSG-deficient cells are extruded toward the basal side and undergo apoptosis. When the direction of delamination is reversed through suppression of RhoGEF2, an activator of the Rho family small GTPases, and JAK/STAT is activated ectopically in these coldspot nTSG-deficient cells, tumorigenesis is induced. These data indicate that two independent processes, apical delamination and JAK/STAT activation, are concurrently required for the initiation of nTSG-deficient-induced tumorigenesis. Given the conservation of the epithelial cytoarchitecture, tumorigenesis may be generally initiated from tumor hotspots by a similar mechanism.
authors: Department of Biological Science, Florida State University, Tallahassee, Florida, United States of America.|Structural Biology Center, National Institute of Genetics and Department of Genetics, School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), 1111 Yata, Mishima, Japan.
authors: Yoichiro
authors: Y
authors: Tamori
authors: Structural Biology Center, National Institute of Genetics and Department of Genetics, School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), 1111 Yata, Mishima, Japan.
authors: Emiko
authors: E
authors: Suzuki
authors: Department of Biological Science, Florida State University, Tallahassee, Florida, United States of America.
authors: Wu-Min
authors: WM
authors: Deng
mesh_terms: D000818:Animals; D063646:Carcinogenesis; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster; D004847:Epithelial Cells / Q000502:physiology; D055785:Gene Knockdown Techniques; D060227:Imaginal Discs / Q000473:pathology; D053612:Janus Kinases / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D009375:Neoplasms, Glandular and Epithelial / Q000473:pathology*; D009928:Organ Specificity; D021381:Protein Transport; D034622:RNA Interference; D050791:STAT Transcription Factors / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism; D059016:Tumor Microenvironment
chemical_list: D029721:Drosophila Proteins; D050791:STAT Transcription Factors; C090223:TSG protein, Drosophila; D014157:Transcription Factors; D053612:Janus Kinases; C085845:hop protein, Drosophila