title: Comparison of two chemiluminescent immunoassays in the detection of measles IgM antibodies.
abstract: Serological confirmation of measles is achieved by detecting the specific immunoglobulin M (IgM), and it is important to evaluate new commercial inmunoassays in order to ensure the quality of results. The objective of this study was to compare the performance of a novel automated chemiluminescent immunoassay (CLIA), Virclia IgM measles (Vircell, Spain), with that of the widely used Liaison measles IgM assay (DiaSorin, Italy). A panel of 86 sera from laboratory-confirmed cases was used for the sensitivity calculation, and 59 sera from healthy individuals and those with other viral infections were used for the specificity calculation. Sensitivity values were 96.5% for Virclia and 97.6% for Liaison; specificity values were 93.2% for Virclia and 96.6% for Liaison; neither difference was statistically significant VirClia IgM measles is a good alternative to other immunoassays for the serological confirmation of measles.
authors: Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: gomezcamarasa@gmail.com.
authors: Cristina
authors: C
authors: Gómez-Camarasa
authors: Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: ana.lara.sspa@juntadeandalucia.es.
authors: Ana
authors: A
authors: Lara-Oya
authors: Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: fernando.cobo.sspa@juntadeandalucia.es.
authors: Fernando
authors: F
authors: Cobo
authors: Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: antonioj.sampedro.sspa@juntadeandalucia.es.
authors: Antonio
authors: A
authors: Sampedro-Martínez
authors: Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: javierm.rodriguez.sspa@juntadeandalucia.es.
authors: Javier
authors: J
authors: Rodríguez-Granger
authors: Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: josegf@ugr.es.
authors: José
authors: J
authors: Gutierrez-Fernández
authors: Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: josem.navarro.sspa@juntadeandalucia.es.
authors: José María
authors: JM
authors: Navarro-Marí
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D007124:Immunoenzyme Techniques / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood*; D007558:Italy; D008163:Luminescent Measurements; D008457:Measles / Q000175:diagnosis* / Q000276:immunology* / Q000821:virology; D008459:Measles virus / Q000276:immunology* / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D013030:Spain
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic
keywords: Chemiluminescent immunoassay; Measles; Serology