title: Use of Baicalin-Conjugated Gold Nanoparticles for Apoptotic Induction of Breast Cancer Cells.
abstract: Baicalin (BC) has been used for cancer therapy due to its multiple effects as an anti-cancer drug. However, the effective delivery of this molecule to targeted cells is difficult. Gold nanoparticles (AuNPs) conjugated with thiolated beta cyclodextrin (AuNP-S-β-CD) were used as a delivery vector in this study. Cell viability tests were evaluated by cell counting kit-8 (CCK) and live/dead cell assay. To demonstrate the proliferation inhibition effect on Michigan Cancer Foundation-7 (MCF-7) cells by BC, we analyzed using Hoechst 33342 staining assay and gel electrophoresis. The S-β-CD conjugated AuNPs were characterized by transmission electron microscopy (TEM), 1H nuclear magnetic resonance ((1)H NMR), and ultraviolet visible (UV-vis) spectroscopy. AuNP-S-β-CD with approximately 40 μM of BC loaded by inclusion complex showed an inhibition effect on MCF-7 cells by inducing apoptosis. Apoptosis test results were evaluated by analyzing the expression of typical apoptic markers such as cleaved caspase-3, full length caspase-3, and apaf-1 in western blot assay. These results demonstrated that AuNP-S-β-CD-BC inhibited the proliferation of cancerous MCF-7 cells by inducing apoptosis. These findings suggested that AuNP-S-β-CD-BC could be a promising agent for chemotherapeutic usage.
authors: Department of Dentistry, Graduate School, Kyung Hee University, Seoul, 02477, Korea.
authors: Donghyun
authors: D
authors: Lee
authors: Department of Dentistry, Graduate School, Kyung Hee University, Seoul, 02477, Korea.
authors: Wan-Kyu
authors: WK
authors: Ko
authors: Department of Clinical Korean Medicine, Graduate School, Kyung Hee Univeristy, Seoul, 02477, Korea.
authors: Deok-Sang
authors: DS
authors: Hwang
authors: Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul, 02477, Korea.
authors: Dong Nyoung
authors: DN
authors: Heo
authors: Department of Dentistry, Graduate School, Kyung Hee University, Seoul, 02477, Korea.
authors: Sang Jin
authors: SJ
authors: Lee
authors: Department of Dentistry, Graduate School, Kyung Hee University, Seoul, 02477, Korea.
authors: Min
authors: M
authors: Heo
authors: Department of Oral and Maxillofacial Radiology, School of Dentistry, Kyung Hee University, Seoul, 02477, Korea.
authors: Kook-Sun
authors: KS
authors: Lee
authors: Department of Clinical Korean Medicine, Graduate School, Kyung Hee Univeristy, Seoul, 02477, Korea.
authors: Ji-Yoon
authors: JY
authors: Ahn
authors: Department of Korean Gynecology, Conmaul Hospital, Kyung Hee University, Seoul, 02477, Korea. studd@naver.com.
authors: Junyoung
authors: J
authors: Jo
authors: Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul, 02477, Korea. kwoni@khu.ac.kr.
authors: Il Keun
authors: IK
authors: Kwon
keywords: Anti-cancer; Apoptosis; Baicalin; Beta cyclodextrin; Gold nanoparticle