title: The N-end rule adaptor protein ClpS from Plasmodium falciparum exhibits broad substrate specificity.
abstract: The N-end rule is a conserved protein degradation pathway that relates the metabolic stability of a protein to the identity of its N-terminal residue. Proteins bearing a destabilising N-terminal residue (N-degron) are recognised by specialised components of the pathway (N-recognins) and degraded by cellular proteases. In bacteria, the N-recognin ClpS is responsible for the specific recognition of proteins bearing an N-terminal destabilising residue such as leucine, phenylalanine, tyrosine or tryptophan. In this study, we show that the putative apicoplast N-recognin from Plasmodium falciparum (PfClpS), in contrast to its bacterial homologues, exhibits an expanded substrate specificity that includes recognition of the branched chain amino acid isoleucine.
authors: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia.
authors: Ju Lin
authors: JL
authors: Tan
authors: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia.
authors: Linda
authors: L
authors: Ward
authors: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia.
authors: Kaye N
authors: KN
authors: Truscott
authors: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia. d.dougan@latrobe.edu.au.
authors: David A
authors: http://orcid.org/0000-0001-9313-1904
authors: DA
authors: Dougan
mesh_terms: D049071:Endopeptidase Clp / Q000737:chemistry / Q000378:metabolism*; D007532:Isoleucine / Q000378:metabolism; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism*; D010961:Plasmodium / Q000201:enzymology*; D000072417:Protein Domains; D059748:Proteolysis; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D009363:Neoplasm Proteins; D015800:Protozoan Proteins; C021081:recognins; D007532:Isoleucine; D049071:Endopeptidase Clp
keywords: 
  Plasmodium falciparum
  ; ClpS; N-end rule