Double-layered cell transfer technology for bone regeneration.
For cell-based medicine, to mimic in vivo cellular localization, various tissue engineering approaches have been studied to obtain a desirable arrangement of cells on scaffold materials. We have developed a novel method of cell manipulation called "cell transfer technology", enabling the transfer of cultured cells onto scaffold materials, and controlling cell topology. Here we show that using this technique, two different cell types can be transferred onto a scaffold surface as stable double layers or in patterned arrangements. Various combinations of adherent cells were transferred to a scaffold, amniotic membrane, in overlapping bilayers (double-layered cell transfer), and transferred cells showed stability upon deformations of the material including folding and trimming. Transplantation of mesenchymal stem cells from periodontal ligaments (PDLSC) and osteoblasts, using double-layered cell transfer significantly enhanced bone formation, when compared to single cell type transplantation. Our findings suggest that this double-layer cell transfer is useful to produce a cell transplantation material that can bear two cell layers. Moreover, the transplantation of an amniotic membrane with PDLSCs/osteoblasts by cell transfer technology has therapeutic potential for bone defects. We conclude that cell transfer technology provides a novel and unique cell transplantation method for bone regeneration.
Periodontology, Department of Hard Tissue Engineering, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Keiko
K
Akazawa
Department of Nanomedicine (DNP), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Kengo
K
Iwasaki
Periodontology, Department of Hard Tissue Engineering, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Mizuki
M
Nagata
Life Science Laboratory, Research and Development Center, Dai Nippon Printing Co., Ltd., 1-1-1 Kaga-cho, Shinjuku-ku, Tokyo, 162-8001, Japan.
Naoki
N
Yokoyama
Life Science Laboratory, Research and Development Center, Dai Nippon Printing Co., Ltd., 1-1-1 Kaga-cho, Shinjuku-ku, Tokyo, 162-8001, Japan.
Hirohito
H
Ayame
Life Science Laboratory, Research and Development Center, Dai Nippon Printing Co., Ltd., 1-1-1 Kaga-cho, Shinjuku-ku, Tokyo, 162-8001, Japan.
Kazumasa
K
Yamaki
Life Science Laboratory, Research and Development Center, Dai Nippon Printing Co., Ltd., 1-1-1 Kaga-cho, Shinjuku-ku, Tokyo, 162-8001, Japan.
Yuichi
Y
Tanaka
Department of Comprehensive Reproductive Medicine, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Izumi
I
Honda
Department of Pediatrics and Developmental Biology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Chikako
C
Morioka
Department of Comprehensive Reproductive Medicine, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Tsuyoshi
T
Kimura
Department of Nanomedicine (DNP), Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Motohiro
M
Komaki
Department of Material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (TMDU), 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Akio
A
Kishida
Periodontology, Department of Hard Tissue Engineering, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Yuichi
Y
Izumi
Department of Cellular Physiological Chemistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Ikuo
I
Morita
D000650:Amnion / Q000637:transplantation; D000818:Animals; D001861:Bone Regeneration; D002454:Cell Differentiation / Q000235:genetics; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D010006:Osteoblasts / Q000637:transplantation*; D010513:Periodontal Ligament / Q000166:cytology / Q000637:transplantation*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds