Effect of Putrescine Treatment on Chilling Injury, Fatty Acid Composition and Antioxidant System in Kiwifruit.
We investigated the effects of different concentrations (0, 1, 2 and 4 mM) of putrescine on chilling injury, fruit quality, ethylene production rate, fatty acid composition and the antioxidant system of cold-stored kiwifruit (Actinidia chinensis Planch. var. chinensis 'Hongyang'). We achieved a significant decrease in ethylene production, maintained fruit quality and alleviated chilling injury during storage via treatment with 2 mM putrescine. Furthermore, putrescine treatment inhibited increases in superoxide anion production rate and H2O2 concentration, while maintaining higher membrane lipid unsaturation as well as increased activities of superoxide dismutase and catalase. In addition, putrescine treatment enhanced the activities of antioxidant enzymes related to the ascorbate-glutathione cycle while causing higher levels of ascorbic acid and reduced glutathione. Our results suggest that induced tolerance against chilling injury via putrescine treatment in cold-stored kiwifruit may be due to enhanced antioxidant activity, increased unsaturation of membrane lipids, and inhibited ethylene production.
College of Horticulture, Northwest A&F University, Yangling, Shaanxi Province, 712100, PR China.|Department of Life Sciences, Yuncheng University, Yuncheng, Shanxi Province, 044000, PR China.
Qingzhen
Q
Yang
Department of Life Sciences, Yuncheng University, Yuncheng, Shanxi Province, 044000, PR China.
Feng
F
Wang
College of Horticulture, Northwest A&F University, Yangling, Shaanxi Province, 712100, PR China.
Jingping
J
Rao
D029042:Actinidia / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003080:Cold Temperature; D005030:Ethylenes / Q000096:biosynthesis; D005227:Fatty Acids / Q000032:analysis*; D005638:Fruit / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D025341:Principal Component Analysis; D011700:Putrescine / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D005030:Ethylenes; D005227:Fatty Acids; D013481:Superoxides; C036216:ethylene; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid; D011700:Putrescine