Central inhibitory effects on feeding induced by the adipo-myokine irisin.
Irisin, the soluble secreted form of fibronectin type III domain containing 5 (FNDC5)-cleaved product, is a recently identified adipo-myokine that has been indicated as a possible link between physical exercise and energetic homeostasis. The co-localization of irisin with neuropeptide Y in hypothalamic sections of paraventricular nucleus, which receives NPY/AgRP projections from the arcuate nucleus, suggests a possible role of irisin in the central regulation of energy balance. In this context, in the present work we studied the effects of intra-hypothalamic irisin (1μl, 50-200nmol/l) administration on feeding and orexigenic [agouti-related peptide (AgRP), neuropeptide Y (NPY) and orexin-A] and anorexigenic [cocaine and amphetamine-regulated transcript (CART) and proopiomelanocortin (POMC)] peptides in male Sprague-Dawley rats. Furthermore, we evaluated the effects of irisin on hypothalamic dopamine (DA), norepinephrine (NE) and serotonin (5-hydroxytryptamine, 5-HT) concentrations and plasma NE levels. Compared to vehicle, irisin injected rats showed decreased food intake, possibly mediated by stimulated CART and POMC and inhibited DA, NE and orexin-A, in the hypothalamus. We also found increased plasma NE levels, supporting a role for sympathetic nervous system stimulation in mediating increased oxygen consumption by irisin.
Department of Pharmacy, "G. d'Annunzio" University, via dei Vestini 31, 66013 Chieti, Italy. Electronic address: cferrante@unich.it.
Claudio
C
Ferrante
Department of Pharmacy, "G. d'Annunzio" University, via dei Vestini 31, 66013 Chieti, Italy. Electronic address: gorlando@unich.it.
Giustino
G
Orlando
Department of Pharmacy, "G. d'Annunzio" University, via dei Vestini 31, 66013 Chieti, Italy. Electronic address: lrecinella@unich.it.
Lucia
L
Recinella
Department of Pharmacy, "G. d'Annunzio" University, via dei Vestini 31, 66013 Chieti, Italy. Electronic address: s.leone@unich.it.
Sheila
S
Leone
Department of Pharmacy, "G. d'Annunzio" University, via dei Vestini 31, 66013 Chieti, Italy. Electronic address: achiavaroli@unich.it.
Annalisa
A
Chiavaroli
Department of Pharmacy, "G. d'Annunzio" University, via dei Vestini 31, 66013 Chieti, Italy. Electronic address: c.dinisio@unich.it.
Chiara
C
Di Nisio
Department of Pharmacy, "G. d'Annunzio" University, via dei Vestini 31, 66013 Chieti, Italy. Electronic address: rugia82@hotmail.it.
Rugia
R
Shohreh
Department of Pharmacy, "G. d'Annunzio" University, via dei Vestini 31, 66013 Chieti, Italy. Electronic address: fabiomanippa@gmail.com.
Fabio
F
Manippa
Department of Pharmacy, "G. d'Annunzio" University, via dei Vestini 31, 66013 Chieti, Italy. Electronic address: adrianaricciuti@virgilio.it.
Adriana
A
Ricciuti
Department of Pharmacy, "G. d'Annunzio" University, via dei Vestini 31, 66013 Chieti, Italy. Electronic address: mvacca@unich.it.
Michele
M
Vacca
Department of Pharmacy, "G. d'Annunzio" University, via dei Vestini 31, 66013 Chieti, Italy. Electronic address: brunetti@unich.it.
Luigi
L
Brunetti
D000818:Animals; D004298:Dopamine / Q000097:blood; D004435:Eating / Q000187:drug effects; D005247:Feeding Behavior / Q000187:drug effects*; D005353:Fibronectins / Q000494:pharmacology*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009479:Neuropeptides / Q000378:metabolism; D009638:Norepinephrine / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000097:blood
C577593:FNDC5 protein, human; D005353:Fibronectins; D009479:Neuropeptides; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine
CART; DA; Irisin; NE; Orexin-A; POMC