Self-assembled silk fibroin nanoparticles loaded with binary drugs in the treatment of breast carcinoma.
Self-assembled nanoparticles of the natural polymer, silk fibroin (SF), are a very promising candidate in drug delivery due to their biocompatible and biodegradable properties. In this study, SF nanoparticles loaded with 5-fluorouracil (5-FU) and curcumin with size 217±0.4 nm and with a loading efficacy of 45% and 15% for 5-FU and curcumin, respectively, were prepared. The in vitro release effect of 5-FU and curcumin from nanoparticles was evaluated as ~100% and ~5%, respectively. It has been revealed that the application of such a nanodrug can increase the level of reactive oxygen species, which in turn induces apoptosis of cancer cells in vitro. Animal studies have shown that tumors could be noticeably reduced after being injected with the drug-entrapped nanoparticles. More apoptotic cells were found after 7 days of treatment with SF nanoparticles by a hematoxylin-eosin staining assay. These results demonstrate the future potential of nanoparticle-loaded binary drugs in the treatment of breast cancer.
School of Biological and Basic Medical Science.
Hui
H
Li
School of Biological and Basic Medical Science; School of Radiological & Interdisciplinary Sciences, Soochow University.
Jian
J
Tian
School of Radiological & Interdisciplinary Sciences, Soochow University.
Anqing
A
Wu
School of Biological and Basic Medical Science.
Jiamin
J
Wang
School of Radiological & Interdisciplinary Sciences, Soochow University.
Cuicui
C
Ge
School of Biological and Basic Medical Science; School of Radiological & Interdisciplinary Sciences, Soochow University; Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou, Jiangsu, People's Republic of China.
Ziling
Z
Sun
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D003474:Curcumin / Q000008:administration & dosage; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D005349:Fibroins / Q000737:chemistry*; D005434:Flow Cytometry; D005472:Fluorouracil / Q000008:administration & dosage; D006801:Humans; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D047011:Silk / Q000737:chemistry*; D023041:Xenograft Model Antitumor Assays
D004337:Drug Carriers; D011108:Polymers; D047011:Silk; D005349:Fibroins; D003474:Curcumin; D005472:Fluorouracil
5-flurouracil; curcumin; reactive oxygen species; silk fibroin nanoparticles