[Feasibility analysis of quantitative detection on serum HBeAg/HBeAb by time-resolved immunofluorescence assay].
OBJECTIVE
To determine whether time-resolved immunofluorescence assay (TRIFA) shares the similar accuracy and specificity with automatic chemiluminescence immunoassay (CMIA) in analyzing HBeAg levels in hepatitis B.


METHODS
A total of 157 serum samples were collected from outpatients with infection of HBV in Xiangya Hospital, Central South University. CMIA and TRIFA were used to analyze HBeAg quantitation and HBeAg/HBeAb qualitative detection, respectively.


RESULTS
The linear regression equation for the two methods was Y=0.72779X-4.0551 (r=0.712, P<0.001). Compared with the CMIA, the sensitivity and specificity in detection of HBeAg by TRIFA were 89.89% and 100%, respectively, and the coincidence rate of HBeAg was 94.27% by two assays. Similarly, the sensitivity and specificity in detection of HBeAb by TRIFA were 100% and 95.45%, respectively. The coincidence rate was 97.45% by two assays.


CONCLUSIONS
TRIFA has similar accuracy, sensitivity, and specificity with CMIA in quantitative detection of HBeAg, and their coincidence rate in detection of HBeAg/HBeAb is high.
Department of Infectious Disease, Xiangya Hospital; Hunan Key Laboratory of Viral Hepatitis, Central South University, Changsha 410008, China.
Xiaoyu
X
Fu
Third Department of Internal Disease, Ganxi Hospital, Pingxiang Jiangxi 337019, China.
Feiyuan
F
Wu
Third Department of Internal Disease, Ganxi Hospital, Pingxiang Jiangxi 337019, China.
Gang
G
Chen
Third Department of Internal Disease, Ganxi Hospital, Pingxiang Jiangxi 337019, China.
Yanling
Y
Xie
Third Department of Internal Disease, Ganxi Hospital, Pingxiang Jiangxi 337019, China.
Guohua
G
Deng
Third Department of Internal Disease, Ganxi Hospital, Pingxiang Jiangxi 337019, China.
Shaojun
S
Gan
Department of Infectious Disease, Xiangya Hospital; Hunan Key Laboratory of Viral Hepatitis, Central South University, Changsha 410008, China.
Lei
L
Fu
D005240:Feasibility Studies; D005455:Fluorescent Antibody Technique; D006509:Hepatitis B; D006510:Hepatitis B Antibodies; D006513:Hepatitis B e Antigens; D006515:Hepatitis B virus; D006801:Humans
D006510:Hepatitis B Antibodies; D006513:Hepatitis B e Antigens