A Phenylurea Cytokinin, CPPU, Elevated Reducing Sugar and Correlated to Andrographolide Contents in Leaves of Andrographis paniculata (Burm. F.) Wall. Ex Nees.
Cytokinins are phytohormones that play multiple roles to control plant growth and development. In this study, leaf biomass and the production of andrographolide compounds in a medicinal plant Andrographis paniculata were significantly increased after exogenously treating with the synthetic cytokinin cytokinin-1-(2-chloro-4-pyridyl)-3-phenylurea (CPPU) at 0 (water), 5, or 10 mg L-1and observed the results for 24 h, 48 h, and 7 days of treatment. It was found that CPPU could significantly enhance new axillary bud formation and further promote branching 4.6-5.6-fold higher, resulting in higher fresh weight (FW) and dry weight (DW) than the control. Application of CPPU at 5 mg L-1significantly promoted the highest contents of total reducing sugar at 2.5-fold in leaves and at 1.5-fold in roots. Although treatments of CPPU significantly affected the increasing contents of chlorophyll and carotenoid (1.2-1.6-fold), CPPU at 10 mg L-1slightly caused leaf stress and chlorophyll reduction. Interestingly, 5 mg L-1CPPU could enhance andrographolide content, an active anti-infectious compound in Andrographis paniculata (2.2-fold higher than the control) that reached the highest content at 24 h after treatment. This study suggested that CPPU should be suitable for field application to promote leaf yields and induce the production of useful pharmaceutical compounds in Andrographis paniculata.
Department of Biotechnology, Faculty of Science, Mahidol University, 272 Rama VI Rd., Ratchathewi, Bangkok, 10400, Thailand.
Phapawee
P
Worakan
School of Interdisciplinary Studies, Mahidol University, Kanchanaburi Campus, Kanchanaburi, Thailand.
Netiya
N
Karaket
Department of Biotechnology, Faculty of Science, Mahidol University, 272 Rama VI Rd., Ratchathewi, Bangkok, 10400, Thailand.
Nuchada
N
Maneejantra
Department of Biotechnology, Faculty of Science, Mahidol University, 272 Rama VI Rd., Ratchathewi, Bangkok, 10400, Thailand. kanyaratt.sup@mahidol.ac.th.
Kanyaratt
K
Supaibulwatana
D035685:Andrographis / Q000502:physiology*; D003583:Cytokinins / Q000008:administration & dosage; D004224:Diterpenes / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D009005:Monosaccharides / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010671:Phenylurea Compounds / Q000008:administration & dosage*; D018515:Plant Leaves / Q000502:physiology*; D013223:Statistics as Topic
D003583:Cytokinins; D004224:Diterpenes; D009005:Monosaccharides; C000597774:N-(2-chloro-4-pyridyl)-N-phenylurea; D010671:Phenylurea Compounds; C030419:andrographolide
Andrographis paniculata; Andrographolide; CPPU; Lateral growth; Reducing sugar