Food-induced changes of lipids in rat neuronal tissue visualized by ToF-SIMS imaging.
Time of flight secondary ion mass spectrometry (ToF-SIMS) was used to image the lipid localization in brain tissue sections from rats fed specially processed cereals (SPC). An IonTof 5 instrument equipped with a Bi cluster ion gun was used to analyze the tissue sections. Data from 15 brain samples from control and cereal-fed rats were recorded and exported to principal components analysis (PCA). The data clearly show changes of certain lipids in the brain following cereal feeding. PCA score plots show a good separation in lipid distribution between the control and the SPC-fed group. The loadings plot reveal that the groups separated mainly due to changes in cholesterol, vitamin E and c18:2, c16:0 fatty acid distribution as well as some short chain monocarboxylic fatty acid compositions. These insights relate to the working mechanism of SPC as a dietary supplement. SPC is thought to activate antisecretory factor (AF), an endogenous protein with regulatory function for inflammation and fluid secretion. These data provide insights into lipid content in brain following SPC feeding and suggest a relation to activating AF.
Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden.|National center for imaging mass spectrometry, Gothenburg, Sweden.
Masoumeh
M
Dowlatshahi Pour
Institute of Biomedicine, Sahlgrenska Academy, Gothenburg, Sweden.
Eva
E
Jennische
Institute of Biomedicine, Sahlgrenska Academy, Gothenburg, Sweden.
Stefan
S
Lange
Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden.|National center for imaging mass spectrometry, Gothenburg, Sweden.|Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Andrew G
AG
Ewing
Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden.|National center for imaging mass spectrometry, Gothenburg, Sweden.
Per
P
Malmberg
D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D002523:Edible Grain / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005502:Food; D007249:Inflammation / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D025341:Principal Component Analysis / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods; D014810:Vitamin E / Q000378:metabolism
D005227:Fatty Acids; D008055:Lipids; D009479:Neuropeptides; C049628:antisecretory factor; D014810:Vitamin E; D002784:Cholesterol