Growth inhibition and apoptotic effects of total flavonoids fromTrollius chinensison human breast cancer MCF-7 cells.
Dried flowers ofTrollius chinensishave long been used as an important traditional Chinese medicine. Previous studies have demonstrated the ability ofT. chinensisflavonoids to reduce the proliferation of human breast cancer MCF-7 cells. The present study further investigated the influence ofT. chinensisflavonoids on the growth and proliferation of MCF-7 cells and observed clear inhibitory effects within the concentration range of 0.0991-1.5856 mg/ml. Apoptosis was triggered byT. chinensisflavonoids treatment that was evaluated by differential interference contrast software, the Hoechst 33258 method, scanning electron microscopy, hematoxylin/eosin staining and laser confocal light microscopy. Cells treated withT. chinensisflavonoids selectively reduced bcl-2 and NF-κB expression and increased the expression of caspase-9 and caspase-3 indicating that the inhibition of cellular proliferation occurred through activation of a mitochondrial pathway. Taken together, the results confirmed the ability ofT. chinensisflavonoids to inhibit cell proliferation.
Department of Pharmacy, Hebei North University, Zhangjiakou, Hebei 075000, P.R. China.
Shuhua
S
Wang
Department of Pharmacy, Hebei North University, Zhangjiakou, Hebei 075000, P.R. China.
Qingqing
Q
Tian
Graduate Faculty, Hebei North University, Zhangjiakou, Hebei 075000, P.R. China.
Fang
F
An
MCF-7; Trollius flavonoids; apoptosis; breast cancer; caspase