1,4-Dioxane degradation potential of members of the genera Pseudonocardia and Rhodococcus.
In recent years, several strains capable of degrading 1,4-dioxane have been isolated from the genera Pseudonocardia and Rhodococcus. This study was conducted to evaluate the 1,4-dioxane degradation potential of phylogenetically diverse strains in these genera. The abilities to degrade 1,4-dioxane as a sole carbon and energy source and co-metabolically with tetrahydrofuran (THF) were evaluated for 13 Pseudonocardia and 12 Rhodococcus species. Pseudonocardia dioxanivorans JCM 13855T, which is a 1,4-dioxane degrading bacterium also known as P. dioxanivorans CB1190, and Rhodococcus aetherivorans JCM 14343Tcould degrade 1,4-dioxane as the sole carbon and energy source. In addition to these two strains, ten Pseudonocardia strains could degrade THF, but no Rhodococcus strains could degrade THF. Of the ten Pseudonocardia strains, Pseudonocardia acacia JCM 16707Tand Pseudonocardia asaccharolytica JCM 10410Tdegraded 1,4-dioxane co-metabolically with THF. These results indicated that 1,4-dioxane degradation potential, including degradation for growth and by co-metabolism with THF, is possessed by selected strains of Pseudonocardia and Rhodococcus, although THF degradation potential appeared to be widely distributed in Pseudonocardia. Analysis of soluble di-iron monooxygenase (SDIMO) α-subunit genes in THF and/or 1,4-dioxane degrading strains revealed that not only THF and 1,4-dioxane monooxygenases but also propane monooxygenase-like SDIMOs can be involved in 1,4-dioxane degradation.
Department of Health Science, Kitasato University, 1-15-1 Kitasato, Sagamihara-Minami, Kanagawa, 252-0373, Japan. d.inoue@kitasato-u.ac.jp.|Environment and Medical Sciences Course, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara-Minami, Kanagawa, 252-0373, Japan. d.inoue@kitasato-u.ac.jp.
Daisuke
http://orcid.org/0000-0002-9216-8198
D
Inoue
Environment and Medical Sciences Course, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara-Minami, Kanagawa, 252-0373, Japan.
Tsubasa
T
Tsunoda
Department of Health Science, Kitasato University, 1-15-1 Kitasato, Sagamihara-Minami, Kanagawa, 252-0373, Japan.
Kazuko
K
Sawada
Technology Center, Taisei Corporation, 344-1 Nase-cho, Totsuka-ku, Yokohama, Kanagawa, 245-0051, Japan.|Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Norifumi
N
Yamamoto
Technology Center, Taisei Corporation, 344-1 Nase-cho, Totsuka-ku, Yokohama, Kanagawa, 245-0051, Japan.
Yuji
Y
Saito
Department of Health Science, Kitasato University, 1-15-1 Kitasato, Sagamihara-Minami, Kanagawa, 252-0373, Japan.|Environment and Medical Sciences Course, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara-Minami, Kanagawa, 252-0373, Japan.
Kazunari
K
Sei
Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Michihiko
M
Ike
D000192:Actinomycetales / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004146:Dioxanes / Q000378:metabolism*; D005663:Furans / Q000378:metabolism; D012240:Rhodococcus / Q000378:metabolism*
D004146:Dioxanes; D005663:Furans; C018674:tetrahydrofuran; C025223:1,4-dioxane
1,4-dioxane degradation; Pseudonocardia; Rhodococcus; Soluble di-iron monooxygenase gene