An in vitro Comparison of Antimicrobial Activity of Three Endodontic Sealers with Different Composition.
OBJECTIVE
The aim of the study was to compare the antimicrobial property of newly introduced EndoSequence BC sealer with commonly used zinc oxide-eugenol-based sealer (Zical) and epoxy resin-based sealer (MM-Seal) against Candida albicans, Lactobacillus, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.


METHODS
The agar diffusion test was done to measure the antimicrobial activity of sealers. The sealers were put in the 4 mm wells prepared in the inoculated agar plates. The agar plates were incubated at 37°C for 24 hours and the zones of inhibition that appeared was measured. Chi-square test was done to evaluate intraobserver bias for all study samples. Intergroup comparison was done for all five parameters using Pearson correlation statistical analysis.


RESULTS
EndoSequence BC sealer showed maximum mean of diameter of zones of inhibition against all the microorganisms but with no statistically significant difference with other sealers. Zical did not show any zone of inhibition against the P. aeruginosa. MM-Seal did not show any inhibitory activity against the P. aeruginosa and C. albicans.


CONCLUSIONS
EndoSequence BC sealer showed antimicrobial activity against all the microorganisms and proved to be a better choice when compared with other two sealers.


CONCLUSIONS
Antimicrobial properties of endodontic sealers are important to prevent the persistent infection of the complex root canals. EndoSequence BC sealer has been proved to be a better sealer in this aspect.
Assistant Professor, Department of Endodontics, College of Dentistry, Jazan University, Jazan, Kingdom of Saudi Arabia, Phone: +966591742345 e-mail: drgurpreetsingh@gmail.com.
Gurpreet
G
Singh
Department of Dental Materials, College of Dentistry, Jazan University, Jazan, Kingdom of Saudi Arabia.
Faheim Mm
FM
Elshamy
Department of Microbiology, College of Dentistry, Jazan University, Jazan, Kingdom of Saudi Arabia.
Husham E
HE
Homeida
Department of Endodontics, College of Dentistry, Jazan University, Jazan, Kingdom of Saudi Arabia.
Nezar
N
Boreak
Department of Endodontics, College of Dentistry, Jazan University, Jazan, Kingdom of Saudi Arabia.
Iti
I
Gupta
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D004338:Drug Combinations; D004853:Epoxy Resins / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D066298:In Vitro Techniques; D007778:Lactobacillus / Q000187:drug effects; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D010087:Oxides / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology*
D000890:Anti-Infective Agents; D002130:Calcium Phosphates; D004338:Drug Combinations; C550603:EndoSequence root repair material; D004853:Epoxy Resins; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement
Candida albicans; Endosequence BC; Escherichia coli; Lactobacillus; Pseudomonas aeruginosa; Staphylococcus aureus.