Novel Anti-Melanogenesis Properties of Polydeoxyribonucleotide, a Popular Wound Healing Booster.
Polydeoxyribonucleotide (PDRN), a deoxyribonucleotide polymer, is popularly used for faster healing of cutaneous wounds and boosting of neocollagenesis of photoaged skin among current dermatologic practitioners. Some patients receiving PDRN injection treatment also reported improvement of photoaging-associated mottled pigmentation (PMP). To investigate the effect of PDRN on cutaneous melanogenesis, we examined the effect of PDRN and an available product (Placentex(®)) containing PDRN on melanogenesis using human melanocytes-keratinocytes cocultures and mouse melanocytes. Melanin content, tyrosinase activity, and levels of microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related protein (TRP-1) were determined. Intracellular signaling pathways were assessed by Western blotting. PDRN and Placentex(®) led to decreases in melanin content, tyrosinase activity, and MITF and TRP-1 expression with concomitant increases in phosphorylated forms of extracellular signal-regulated protein kinase (ERK) and AKT in mouse melanocytes. More importantly, both PDRN and Placentex(®) significantly suppressed the melanin content in human melanocyte-keratinocyte cocultures. Clinical evaluation of six female patients with facial hyperpigmentation after three sessions of intradermal PDRN injections using a 5-point scale revealed that PDRN led to more than noticeable improvements in hyperpigmented lesions. This is the first study to demonstrate that PDRN, which is known for its wound-healing properties, may have novel anti-melanogenesis and potential skin whitening properties.
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea. medsnutk@gmail.com.
Tai Kyung
TK
Noh
Department of Dermatology, College of medicine, Hallym University Kangnam Sacred Heart Hospital, Seoul 07441, Korea. victoryby777@gmail.com.
Bo Young
BY
Chung
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea. u2u2star@gmail.com.
Su Yeon
SY
Kim
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea. algp_@naver.com.
Mi Hye
MH
Lee
MJ All Skin Clinic, Seoul 04537, Korea. mjallskin@gmail.com.
Moon Jung
MJ
Kim
YeMiWon Clinic, Seoul 06280, Korea. dermacsyoun@hanmail.net.
Choon Shik
CS
Youn
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea. miumiu@amc.seoul.kr.
Mi Woo
MW
Lee
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea. csesnumd@gmail.com.
Sung Eun
SE
Chang
D000328:Adult; D000368:Aged; D000818:Animals; D002460:Cell Line; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D008543:Melanins / Q000378:metabolism; D008544:Melanocytes / Q000187:drug effects*; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000008:administration & dosage / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D062327:Skin Lightening Preparations / Q000008:administration & dosage / Q000494:pharmacology*; D012880:Skin Pigmentation / Q000187:drug effects*; D014357:Trypsin / Q000235:genetics / Q000378:metabolism
C497234:MITF protein, human; D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; D011089:Polydeoxyribonucleotides; D062327:Skin Lightening Preparations; D014442:Monophenol Monooxygenase; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; C489303:PRSS1 protein, human; D014357:Trypsin
coculture; hyperpigmentation; melanogenesis; polydeoxyribonucleotide