A Combinatorial Protein Microarray for Probing Materials Interaction with Pancreatic Islet Cell Populations.
Pancreatic islet transplantation has become a recognized therapy for insulin-dependent diabetes mellitus. During isolation from pancreatic tissue, the islet microenvironment is disrupted. The extracellular matrix (ECM) within this space not only provides structural support, but also actively signals to regulate islet survival and function. In addition, the ECM is responsible for growth factor presentation and sequestration. By designing biomaterials that recapture elements of the native islet environment, losses in islet function and number can potentially be reduced. Cell microarrays are a high throughput screening tool able to recreate a multitude of cellular niches on a single chip. Here, we present a screening methodology for identifying components that might promote islet survival. Automated fluorescence microscopy is used to rapidly identify islet derived cell interaction with ECM proteins and immobilized growth factors printed on arrays. MIN6 mouse insulinoma cells, mouse islets and, finally, human islets are progressively screened. We demonstrate the capability of the platform to identify ECM and growth factor protein candidates that support islet viability and function and reveal synergies in cell response.
Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, University of South Australia, Adelaide 5095 SA, Australia. Bahman.Delalat@unisa.edu.au.
Bahman
B
Delalat
School of Medicine, University of Adelaide, Adelaide5005 SA, Australia. Darling.Rojas@sa.gov.au.|Centre for Clinical and Experimental Transplantation, Adelaide 5000 SA, Australia. Darling.Rojas@sa.gov.au.
Darling M
DM
Rojas-Canales
Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, University of South Australia, Adelaide 5095 SA, Australia. rasighaemis@yahoo.com.
Soraya
S
Rasi Ghaemi
Immunology and Diabetes Unit, St. Vincent's Institute of Medical Research, Fitzroy 3065 Vic, Australia. mwaibel@svi.edu.au.
Michaela
M
Waibel
Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, University of South Australia, Adelaide 5095 SA, Australia. Fran.Harding@unisa.edu.au.
Frances J
FJ
Harding
School of Medicine, University of Adelaide, Adelaide5005 SA, Australia. Daniella.Penko@sa.gov.au.|Centre for Clinical and Experimental Transplantation, Adelaide 5000 SA, Australia. Daniella.Penko@sa.gov.au.|Central Northern Adelaide Renal Transplantation Service, Royal Adelaide Hospital, Adelaide 5000 SA, Australia. Daniella.Penko@sa.gov.au.
Daniella
D
Penko
School of Medicine, University of Adelaide, Adelaide5005 SA, Australia. Chris.Drogemuller@sa.gov.au.|Centre for Clinical and Experimental Transplantation, Adelaide 5000 SA, Australia. Chris.Drogemuller@sa.gov.au.|Central Northern Adelaide Renal Transplantation Service, Royal Adelaide Hospital, Adelaide 5000 SA, Australia. Chris.Drogemuller@sa.gov.au.
Christopher J
CJ
Drogemuller
Immunology and Diabetes Unit, St. Vincent's Institute of Medical Research, Fitzroy 3065 Vic, Australia. tloudovaris@svi.edu.au.
Thomas
T
Loudovaris
School of Medicine, University of Adelaide, Adelaide5005 SA, Australia. Toby.Coates@sa.gov.au.|Centre for Clinical and Experimental Transplantation, Adelaide 5000 SA, Australia. Toby.Coates@sa.gov.au.|Central Northern Adelaide Renal Transplantation Service, Royal Adelaide Hospital, Adelaide 5000 SA, Australia. Toby.Coates@sa.gov.au.
Patrick T H
PT
Coates
Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, University of South Australia, Adelaide 5095 SA, Australia. Nico.Voelcker@unisa.edu.au.
Nicolas H
NH
Voelcker
ECM proteins; high throughput screening; microarrays; pancreatic islets