Holographic intravital microscopy for 2-D and 3-D imaging intact circulating blood cells in microcapillaries of live mice.
Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging cells in vivo have been proposed, most require the use of labelling and also provide only qualitative imaging information. Holographic imaging approach based on measuring the refractive index distributions of cells, however, circumvent these problems and offer quantitative and label-free imaging capability. Here, we demonstrate in vivo two- and three-dimensional holographic imaging of circulating blood cells in intact microcapillaries of live mice. The measured refractive index distributions of blood cells provide morphological and biochemical properties including three-dimensional cell shape, haemoglobin concentration, and haemoglobin contents at the individual cell level. With the present method, alterations in blood flow dynamics in live healthy and sepsis-model mice were also investigated.
Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.|KI for Health Science and Technology (KIHST), KAIST, Daejeon 34141, Republic of Korea.
Kyoohyun
K
Kim
KI for Health Science and Technology (KIHST), KAIST, Daejeon 34141, Republic of Korea.|Graduate School of Nanoscience and Technology, KAIST, Daejeon 34141, Republic of Korea.
Kibaek
K
Choe
KI for Health Science and Technology (KIHST), KAIST, Daejeon 34141, Republic of Korea.|Graduate School of Medical Science and Engineering, KAIST, Daejeon 34141, Republic of Korea.
Inwon
I
Park
KI for Health Science and Technology (KIHST), KAIST, Daejeon 34141, Republic of Korea.|Graduate School of Nanoscience and Technology, KAIST, Daejeon 34141, Republic of Korea.
Pilhan
P
Kim
Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.|KI for Health Science and Technology (KIHST), KAIST, Daejeon 34141, Republic of Korea.|Tomocube, Inc., Daejeon 34051, Republic of Korea.
YongKeun
Y
Park
D000818:Animals; D001773:Blood Cells / Q000166:cytology*; D002196:Capillaries / Q000166:cytology*; D048430:Cell Shape / Q000502:physiology; D006696:Holography / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods; D000069416:Intravital Microscopy / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D012031:Refractometry / Q000379:methods