Synthesis and characterization of silver doped hydroxyapatite nanocomposite coatings and evaluation of their antibacterial and corrosion resistance properties in simulated body fluid.
Silver-doped hydroxyapatite (Ca10-xAgx(PO4)6(OH)2-x) films were synthesized and deposited on anodized titanium (Ti) using electrophoretic. The influence of different silver-dopant contents (X=0, 0.02, 0.05, 0.08 and 0.1) on the phase formation and microstructure of the powders were characterized by means of X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscope (XPS), and Fourier transform infrared spectrum analysis (FT-IR). XRD analysis confirmed the formation of Hexagonal structure of hydroxyapatite (HAp) annealed at 600°C with a small shift in the major peak position toward lower angles with adding silver. FT-IR spectroscopy disclosed the presence of the different vibrational modes matching to phosphates and hydroxyl groups and the absence of any band characteristics to silver. XPS analysis showed that 75% and 23% of silver was in the chemical states of Ag(2+) and Ag(+), respectively. However, only about 2% of silver was in the Ag(0) state, resulting in the high quality of nanocomposite films. The anodization treatment improves the bond strength between the Ag doped HAp deposited layers on TiO2. HAp and silver doped HAp (X=0.05) are regarded to be hydrophilic due to a large number of -OH groups on the surface. The sample with content of silver (x=0.05) also showed excellent antimicrobial efficacy (>99% reduction in viable cells). Electrochemical reveals the passive current densities of the HAp coated anodized Ti are lower than those of silver doped HAp coated anodized Ti, leading to a slightly lower corrosion resistance.
School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box 11155-4563, Tehran, Iran. Electronic address: majidmirzaee7@gmail.com.
Majid
M
Mirzaee
Research Department of Nano-Technology and Advanced Materials, Materials & Energy Research Center, Iran.
Mohammadreza
M
Vaezi
Research Department of Nano-Technology and Advanced Materials, Materials & Energy Research Center, Iran.
Yahya
Y
Palizdar
D000268:Adhesiveness; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001826:Body Fluids; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D015169:Colony Count, Microbial; D003343:Corrosion; D058266:Dielectric Spectroscopy; D017886:Durapatite / Q000138:chemical synthesis*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D012834:Silver / Q000494:pharmacology*; D012996:Solutions; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D012996:Solutions; D012834:Silver; D017886:Durapatite
Corrosion stability; Electrophoretic; Sliver doped hydroxyapatite; TiO(2) nanotubes