Changes of Regulatory T Cells in the Early Stage of Obesity Mice and Their Modulation on Macrophage Subtypes in Visceral Adipose Tissue.
Objective To investigate the changes of regulatory T cells (Tregs) and whether Tregs can modulate the distribution of macrophage subtypes in visceral adipose tissue in the early stage of obesity.Methods After C57BL/6 mice obesity models were successfully established,metabolic parameters and numbers of Tregs and M1/M2 macrophage were measured at 4,10,and 20 weeks.The changes of metabolic parameters and adipose tissue inflammation in obesity mice after rapamycin intervention were evaluated. Results The early-stage obesity models were successfully established.Compared with normal diet mice,high fat diet mice had significantly higher epididymal adipose tissue mass and serum leptin levels(P<0.05).However,there was no statistical difference in blood glucose and insulin levels between these two groups(All P>0.05). Macrophages infiltration in adipose tissue in high fat diet mice gradually increased with time,coincident with decrease in Treg numbers. Increased numbers of Treg,improved metabolic parameters,and decreased ratio of M1/M2 can be seen after rapamycin intervention in mice.Conclusion The decrease of Tregs in the early stage of obesity may contribute to abnormal distribution of macrophage subtypes in visceral adipose.
Department of Metabolism and Endocrinology,the Second Xiangya Hospital,Central South University,Key Laboratory of Diabetes Immunology,Ministry of Education,Central South University,National Clinical Research Center for Metabolic Diseases,Changsha 410011,China.
Xia
X
Li
Department of Metabolism and Endocrinology,the Second Xiangya Hospital,Central South University,Key Laboratory of Diabetes Immunology,Ministry of Education,Central South University,National Clinical Research Center for Metabolic Diseases,Changsha 410011,China.
Xiao-Han
XH
Tang
Department of Metabolism and Endocrinology,the Second Xiangya Hospital,Central South University,Key Laboratory of Diabetes Immunology,Ministry of Education,Central South University,National Clinical Research Center for Metabolic Diseases,Changsha 410011,China.
Li-Li
LL
Tang
Department of Metabolism and Endocrinology,the Second Xiangya Hospital,Central South University,Key Laboratory of Diabetes Immunology,Ministry of Education,Central South University,National Clinical Research Center for Metabolic Diseases,Changsha 410011,China.
Hai-Bo
HB
Yu
Department of Metabolism and Endocrinology,the Second Xiangya Hospital,Central South University,Key Laboratory of Diabetes Immunology,Ministry of Education,Central South University,National Clinical Research Center for Metabolic Diseases,Changsha 410011,China.
Zhi-Guo
ZG
Xie
Department of Metabolism and Endocrinology,the Second Xiangya Hospital,Central South University,Key Laboratory of Diabetes Immunology,Ministry of Education,Central South University,National Clinical Research Center for Metabolic Diseases,Changsha 410011,China.
Zhi-Guang
ZG
Zhou
D000818:Animals; D001786:Blood Glucose; D059305:Diet, High-Fat; D007249:Inflammation; D050152:Intra-Abdominal Fat / Q000166:cytology*; D020738:Leptin / Q000097:blood; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000166:cytology*
D001786:Blood Glucose; D020738:Leptin