Hydrogen Sulfide Alleviates Postharvest Senescence of Grape by Modulating the Antioxidant Defenses.
Hydrogen sulfide (H2S) has been identified as an important gaseous signal in plants. Here, we investigated the mechanism of H2S in alleviating postharvest senescence and rotting of Kyoho grape. Exogenous application of H2S released from 1.0 mM NaHS remarkably decreased the rotting and threshing rate of grape berries. H2S application also prevented the weight loss in grape clusters and inhibited the decreases in firmness, soluble solids, and titratable acidity in grape pulp during postharvest storage. The data of chlorophyll and carotenoid content suggested the role of H2S in preventing chlorophyll breakdown and carotenoid accumulation in both grape rachis and pulp. In comparison to water control, exogenous H2S application maintained significantly higher levels of ascorbic acid and flavonoid and total phenolics and reducing sugar and soluble protein in grape pulp. Meanwhile, H2S significantly reduced the accumulation of malondialdehyde (MDA), hydrogen peroxide (H2O2), and superoxide anion (O2 (∙-)) in grape pulp. Further investigations showed that H2S enhanced the activities of antioxidant enzymes ascorbate peroxidase (APX) and catalase (CAT) and decreased those of lipoxygenase (LOX) in both grape peels and pulp. In all, we provided strong evidence that H2S effectively alleviated postharvest senescence and rotting of Kyoho grape by modulating antioxidant enzymes and attenuating lipid peroxidation.
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China; Biological Science and Engineering College, Beifang University of Nationalities, Yinchuan 750021, China.
Zhi-Jing
ZJ
Ni
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Kang-Di
KD
Hu
Biological Science and Engineering College, Beifang University of Nationalities, Yinchuan 750021, China.
Chang-Bing
CB
Song
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China; Biological Science and Engineering College, Beifang University of Nationalities, Yinchuan 750021, China.
Run-Hui
RH
Ma
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Zhi-Rong
ZR
Li
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Ji-Lian
JL
Zheng
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Liu-Hui
LH
Fu
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Zhao-Jun
0000-0003-1729-209X
ZJ
Wei
School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Hua
0000-0002-8406-8373
H
Zhang
D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D002338:Carotenoids / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D016922:Cellular Senescence / Q000187:drug effects*; D002734:Chlorophyll / Q000378:metabolism; D018556:Crops, Agricultural; D005419:Flavonoids / Q000378:metabolism; D005638:Fruit / Q000187:drug effects* / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008084:Lipoxygenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013440:Sulfides / Q000494:pharmacology*; D013997:Time Factors; D027843:Vitis / Q000187:drug effects* / Q000378:metabolism
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D013440:Sulfides; D002734:Chlorophyll; D002338:Carotenoids; D008315:Malondialdehyde; D060387:Ascorbate Peroxidases; D002374:Catalase; D008084:Lipoxygenase; C025451:sodium bisulfide; D001205:Ascorbic Acid; D006862:Hydrogen Sulfide