Applications of CRISPR Genome Engineering in Cell Biology.
Recent advances in genome engineering are starting a revolution in biological research and translational applications. The clustered regularly interspaced short palindromic repeats (CRISPR)-associated RNA-guided endonuclease CRISPR associated protein 9 (Cas9) and its variants enable diverse manipulations of genome function. In this review, we describe the development of Cas9 tools for a variety of applications in cell biology research, including the study of functional genomics, the creation of transgenic animal models, and genomic imaging. Novel genome engineering methods offer a new avenue to understand the causality between the genome and phenotype, thus promising a fuller understanding of cell biology.
Sino-U.S. Center of Synthetic Biology, Shanghai Institute of Rheumatology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Fangyuan
F
Wang
Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; ChEM-H, Stanford University, Stanford, CA 94305, USA. Electronic address: stanley.qi@stanford.edu.
Lei S
LS
Qi
D000818:Animals; D064113:CRISPR-Cas Systems / Q000235:genetics*; D003585:Cell Biology; D044127:Epigenesis, Genetic; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome; D006801:Humans; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000378:metabolism
D000094704:RNA, Guide, CRISPR-Cas Systems
CRISPR/Cas9; CRISPRi/a; cell biology; gene editing; gene regulation