Inside-out Ultraviolet-C Sterilization of Pseudomonas aeruginosa Biofilm In Vitro.
Biofilms are difficult to eradicate due to a protective architecture and create major challenges in patient care by diminishing both host immune response and therapeutic approaches. This study investigated a new strategy for treating surface-attached biofilms by delivering germicidal UV through a material surface in a process referred to as "inside-out sterilization" (IOS). Mature Pseudomonas aeruginosa (ATCC®27853™) biofilms were irradiated with up to 1400 mJ cm-2of germicidal UV from both ambient and IOS configurations. The lethal dose for the ambient exposure group was 461 mJ cm-295% CI [292, 728] compared to the IOS treatment group of 247 mJ cm-295% CI [187, 325], corresponding to 47% less UV dosage for the IOS group (P < 0.05). This study demonstrated that with IOS, a lower quantal dosage of UV energy is required to eradicate biofilm than with ambient exposure by leveraging the organizational structure of the biofilm.
The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD.
Cameron C
0000-0002-0142-6586
CC
Jones
The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD.
Steffi
S
Valdeig
Photonics Division, Johns Hopkins University Applied Physics Lab, Laurel, MD.
Raymond M
RM
Sova
The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD.
Clifford R
CR
Weiss
D018441:Biofilms / Q000528:radiation effects*; D066298:In Vitro Techniques; D007928:Lethal Dose 50; D011550:Pseudomonas aeruginosa / Q000528:radiation effects*; D013242:Sterilization / Q000379:methods*; D014466:Ultraviolet Rays