Building a Three-Dimensional Nano-Bio Interface for Aptasensing: An Analytical Methodology Based on Steric Hindrance Initiated Signal Amplification Effect.
The development of novel detection methodologies in electrochemiluminescence (ECL) aptasensor fields with simplicity and ultrasensitivity is essential for constructing biosensing architectures. Herein, a facile, specific, and sensitive methodology was developed unprecedentedly for quantitative detection of microcystin-LR (MC-LR) based on three-dimensional boron and nitrogen codoped graphene hydrogels (BN-GHs) assisted steric hindrance amplifying effect between the aptamer and target analytes. The recognition reaction was monitored by quartz crystal microbalance (QCM) to validate the possible steric hindrance effect. First, the BN-GHs were synthesized via self-assembled hydrothermal method and then applied as the Ru(bpy)32+immobilization platform for further loading the biomolecule aptamers due to their nanoporous structure and large specific surface area. Interestingly, we discovered for the first time that, without the aid of conventional double-stranded DNA configuration, such three-dimensional nanomaterials can directly amplify the steric hindrance effect between the aptamer and target analytes to a detectable level, and this facile methodology could be for an exquisite assay. With the MC-LR as a model, this novel ECL biosensor showed a high sensitivity and a wide linear range. This strategy supplies a simple and versatile platform for specific and sensitive determination of a wide range of aptamer-related targets, implying that three-dimensional nanomaterials would play a crucial role in engineering and developing novel detection methodologies for ECL aptasensing fields.
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, and ‡School of Food and Biological Engineering, Jiangsu University , Zhenjiang, Jiangsu 212013, People's Republic of China.
Xiaojiao
X
Du
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, and ‡School of Food and Biological Engineering, Jiangsu University , Zhenjiang, Jiangsu 212013, People's Republic of China.
Ding
D
Jiang
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, and ‡School of Food and Biological Engineering, Jiangsu University , Zhenjiang, Jiangsu 212013, People's Republic of China.
Nan
N
Hao
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, and ‡School of Food and Biological Engineering, Jiangsu University , Zhenjiang, Jiangsu 212013, People's Republic of China.
Jing
J
Qian
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, and ‡School of Food and Biological Engineering, Jiangsu University , Zhenjiang, Jiangsu 212013, People's Republic of China.
Liming
L
Dai
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, and ‡School of Food and Biological Engineering, Jiangsu University , Zhenjiang, Jiangsu 212013, People's Republic of China.
Lei
L
Zhou
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, and ‡School of Food and Biological Engineering, Jiangsu University , Zhenjiang, Jiangsu 212013, People's Republic of China.
Jianping
J
Hu
Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, and ‡School of Food and Biological Engineering, Jiangsu University , Zhenjiang, Jiangsu 212013, People's Republic of China.
Kun
K
Wang
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D008163:Luminescent Measurements; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques
D052157:Aptamers, Nucleotide; D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR