Shoot-level terpenoids emission in Norway spruce (Picea abies) under natural field and manipulated laboratory conditions.
Norway spruce (Picea abies) is a strong emitter of biogenic volatile organic compounds (BVOCs). In the present study we investigated how shoot canopy position and high levels of stressors such as high temperature and ozone concentration, affect BVOC emission rates by means of in-situ and ex-situ experimental measurements. Therefore, BVOC emission from current-year spruce shoots was investigated under field and manipulated (temperature, ozone) laboratory conditions. Emitted BVOCs were sampled on desorption tubes, coupled with gas-exchange measurements of CO2assimilation rate and stomatal conductance, and detected by gas chromatography coupled with mass spectrometry. Total BVOC emission rates from sun shoots under standard conditions were higher than those from shade shoots, although this was significant only in July, on the contrary, only α-pinene and γ-terpinene emission rates showed significant differences between sun and shade acclimated shoots in August. Limonene, α-pinene, β-pinene, and myrcene were identified as the most abundant BVOCs in both campaigns with emission rates above 0.2 nmol m-2s-1. Ex-situ measurements revealed a significantly higher total BVOC emissions under high temperature level (40 °C) by ca. 175% as compared with standard temperature (30 °C), while a short-term fumigation of acute O3concentration (200 ppb) had no effect on BVOC emissions and its spectrum. These findings might have a relevance considering the role of these compounds in protecting against oxidative stress and their possible stimulation in particular stressful conditions. Implication of such results into emission models may contribute to a more accurate estimation of BVOC emissions for Central European mountain regions dominated by Norway spruce forests and their rate under predicted climate change.
Institute of Agro-Environmental & Forest Biology (IBAF), National Research Council (CNR), Via Marconi 2, IT-05010 Porano, TR, Italy; Department for Innovation in Biological, Agro-food and Forest Systems, University of Tuscia, Via San Camillo de Lellis, IT-01100 Viterbo, Italy.
Raffaela
R
Esposito
Institute of Agro-Environmental & Forest Biology (IBAF), National Research Council (CNR), Via Marconi 2, IT-05010 Porano, TR, Italy; Department for Innovation in Biological, Agro-food and Forest Systems, University of Tuscia, Via San Camillo de Lellis, IT-01100 Viterbo, Italy.
Ilaria
I
Lusini
Laboratory of Ecological Plant Physiology, Global Change Research Institute, Czech Academy of Sciences, Bělidla 986/4a, CZ-603 00 Brno, Czech Republic.
Kristýna Večeřová
Laboratory of Ecological Plant Physiology, Global Change Research Institute, Czech Academy of Sciences, Bělidla 986/4a, CZ-603 00 Brno, Czech Republic.
Petra Holišová
Institute of Agro-Environmental & Forest Biology (IBAF), National Research Council (CNR), Via Marconi 2, IT-05010 Porano, TR, Italy.
Emanuele
E
Pallozzi
Institute of Agro-Environmental & Forest Biology (IBAF), National Research Council (CNR), Via Marconi 2, IT-05010 Porano, TR, Italy.
Gabriele
G
Guidolotti
Laboratory of Ecological Plant Physiology, Global Change Research Institute, Czech Academy of Sciences, Bělidla 986/4a, CZ-603 00 Brno, Czech Republic.
Otmar
O
Urban
Institute of Agro-Environmental & Forest Biology (IBAF), National Research Council (CNR), Via Marconi 2, IT-05010 Porano, TR, Italy; Laboratory of Ecological Plant Physiology, Global Change Research Institute, Czech Academy of Sciences, Bělidla 986/4a, CZ-603 00 Brno, Czech Republic. Electronic address: carlo.calfapietra@ibaf.cnr.it.
Carlo
C
Calfapietra
D002245:Carbon Dioxide / Q000032:analysis / Q000378:metabolism; D018153:Czech Republic; D010126:Ozone; D028222:Picea / Q000502:physiology*; D018520:Plant Shoots / Q000378:metabolism*; D013696:Temperature; D013729:Terpenes / Q000378:metabolism*; D014197:Trees / Q000502:physiology; D055549:Volatile Organic Compounds / Q000032:analysis / Q000378:metabolism
D013729:Terpenes; D055549:Volatile Organic Compounds; D002245:Carbon Dioxide; D010126:Ozone
BVOC; Climate change; Monoterpenes; Norway spruce; Ozone; Stress; Sun-shade; Temperature