Electrical stimulation inhibits cytosine arabinoside-induced neuronal death by preventing apoptosis in dorsal root ganglion neurons.
The current study aimed to investigate whether electrical stimulation could prevent apoptotic neuronal cell death during treatment with cytosine arabinoside (ara-C). From in-vitro experiments, the effects of electrical stimulation were assessed on neurite fragmentation and neuronal cell death in ara-C-treated dorsal root ganglion (DRG) explants. Ara-C treatment increased neurite fragmentation and neuronal cell death in DRG explants and activated caspase-3 by cleaving it, which could induce apoptosis. Electrical stimulation can significantly reduce neurite fragmentation and neuronal cell death compared with nonelectrically stimulated groups. Furthermore, electrical stimulation inhibited caspase-3 activation and reduced apoptotic neuronal death in DRG explants. It was suggested that the neuroprotective effect of electrical stimulation is likely mediated by the inhibition of caspase-3 activation and therefore the inhibition of apoptosis following ara-C treatment.
aBiomedical Research Institute, Korea Institute of Science and Technology bDepartment of Biomedical Science, Korea University College of Medicine, Seoul cDepartment of Biomedical Engineering, Korea University of Science and Technology, Daejeon, Korea.
Sungmin
S
Han
Donghwee
D
Kim
Hyungmin
H
Kim
Jong Woong
JW
Park
Inchan
I
Youn
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology*; D004558:Electric Stimulation / Q000379:methods*; D005727:Ganglia, Spinal / Q000166:cytology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D051379:Mice; D008813:Mice, Inbred ICR; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D009924:Organ Culture Techniques
D007166:Immunosuppressive Agents; D003561:Cytarabine; D053148:Caspase 3