High protein flexibility and reduced hydration water dynamics are key pressure adaptive strategies in prokaryotes.
Water and protein dynamics on a nanometer scale were measured by quasi-elastic neutron scattering in the piezophile archaeon Thermococcus barophilus and the closely related pressure-sensitive Thermococcus kodakarensis, at 0.1 and 40 MPa. We show that cells of the pressure sensitive organism exhibit higher intrinsic stability. Both the hydration water dynamics and the fast protein and lipid dynamics are reduced under pressure. In contrast, the proteome of T. barophilus is more pressure sensitive than that of T. kodakarensis. The diffusion coefficient of hydration water is reduced, while the fast protein and lipid dynamics are slightly enhanced with increasing pressure. These findings show that the coupling between hydration water and cellular constituents might not be simply a master-slave relationship. We propose that the high flexibility of the T. barophilus proteome associated with reduced hydration water may be the keys to the molecular adaptation of the cells to high hydrostatic pressure.
Institut Laue Langevin, F-38042 Grenoble Cedex 9, France.|Univ. Grenoble Alpes, IBS, F-38041 Grenoble Cedex 9, France.
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N
Martinez
Univ. Brest, CNRS, Ifremer, LM2E, IUEM, 29280, Plouzané, France.
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G
Michoud
Univ Lyon, ENS Lyon, CNRS UMR 5276, F - 69364 Lyon cedex 07, France.
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A
Cario
Institut Laue Langevin, F-38042 Grenoble Cedex 9, France.
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J
Ollivier
Univ. Grenoble Alpes, IBS, F-38041 Grenoble Cedex 9, France.
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B
Franzetti
Univ. Brest, CNRS, Ifremer, LM2E, IUEM, 29280, Plouzané, France.
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M
Jebbar
Univ Lyon, ENS Lyon, CNRS UMR 5276, F - 69364 Lyon cedex 07, France.|Univ Lyon, INSA Lyon, CNRS UMR 5240, F - 69621 Villeurbanne cedex, France.
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P
Oger
Institut Laue Langevin, F-38042 Grenoble Cedex 9, France.|Univ. Grenoble Alpes, LiPhy, F-38041 Grenoble Cedex 9, France.
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J
Peters
D019843:Archaeal Proteins / Q000737:chemistry / Q000502:physiology*; D011312:Pressure; D019712:Thermococcus / Q000502:physiology*; D014867:Water / Q000737:chemistry
D019843:Archaeal Proteins; D014867:Water