Temoporfin-loaded 1-tetradecanol-based thermoresponsive solid lipid nanoparticles for photodynamic therapy.
We developed fully biodegradable/metabolizable nanosystem based on polymer surfactant-stabilized thermoresponsive solid lipid nanoparticles with non-covalently bound photosensitizer temoporfin (T-SLNP) with particle size below 50nm. The efficacy of T-SLNP was compared with commercial temoporfin formulation in terms of in vitro phototoxicity in 4T1 (murine mammary carcinoma) and MDA-MB-231(human breast adenocarcinoma) cells and of in vivo anticancer effect in Nu/Nu mice bearing MDA-MB-231 tumors. In vitro study demonstrated faster accumulation kinetics in the cells for our formulation design resulting in higher phototoxicity against the tumor cells. In vivo anticancer efficacy was markedly improved by T-SLNP compared with commercial temoporfin formulation. Owing to controlled and sustained release properties, subcellular size, biocompatibility with tissue and cells, the T-SLNP nanodispersion prepared in this study represents promising drug delivery system applicable in cancer treatment.
Faculty of Chemical Engineering, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic.
Ingrid
I
Brezaniova
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i, Heyrovskéhonám. 2, 162 06 Prague 6, Czech Republic. Electronic address: mhruby@centrum.cz.
Martin
M
Hruby
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videňská 1083, 142 20 Prague 4, Czech Republic.
Jarmila
J
Kralova
Faculty of Chemical Engineering, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague 6, Czech Republic.
Vladimir
V
Kral
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i, Heyrovskéhonám. 2, 162 06 Prague 6, Czech Republic.
Zulfiya
Z
Cernochova
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i, Heyrovskéhonám. 2, 162 06 Prague 6, Czech Republic.
Peter
P
Cernoch
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i, Heyrovskéhonám. 2, 162 06 Prague 6, Czech Republic.
Miroslav
M
Slouf
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i, Heyrovskéhonám. 2, 162 06 Prague 6, Czech Republic.
Jana
J
Kredatusova
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i, Heyrovskéhonám. 2, 162 06 Prague 6, Czech Republic.
Petr
P
Stepanek
D000818:Animals; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry*; D005233:Fatty Alcohols / Q000008:administration & dosage* / Q000627:therapeutic use; D005260:Female; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008325:Mammary Neoplasms, Experimental / Q000628:therapy; D008652:Mesoporphyrins / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000627:therapeutic use
D004337:Drug Carriers; D005233:Fatty Alcohols; D008055:Lipids; D008652:Mesoporphyrins; D017319:Photosensitizing Agents; C072269:temoporfin; C020940:myristyl alcohol
Drug delivery; Nanomedicine; Photodynamic therapy; Solid lipid nanoparticles; Temoporfin