Antiviral activities of atractylon from Atractylodis Rhizoma.
Atractylodis Rhizoma is a traditional medicinal herb, which has antibacterial, antiviral, anti‑inflammatory and anti‑allergic, anticancer, gastroprotective and neuroprotective activities. It is widely used for treating fever, cold, phlegm, edema and arthralgia syndrome in South‑East Asian nations. In this study, 6 chemical compositions of Atractylodis Rhizoma were characterized by spectral analysis and their antiviral activities were evaluated in vitro and in vivo. Among them, atractylon showed most significant antiviral activities. Atractylon treatment at doses of 10‑40 mg/kg for 5 days attenuated influenza A virus (IAV)‑induced pulmonary injury and decreased the serum levels of interleukin (IL)‑6, tumor necrosis factor‑α and IL‑1β, but increased interferon‑β (IFN‑β) levels. Atractylon treatment upregulated the expression of Τoll‑like receptor 7 (TLR7), MyD88, tumor necrosis factor receptor‑associated factor 6 and IFN‑β mRNA but downregulated nuclear factor‑κB p65 protein expression in the lung tissues of IAV‑infected mice. These results demonstrated that atractylon significantly alleviated IAV‑induced lung injury via regulating the TLR7 signaling pathway, and may warrant further evaluation as a possible agent for IAV treatment.
Department of Infectious Disease, Hospital for Infectious Diseases of Pudong New Area, Shanghai 201299, P.R. China.
Yang
Y
Cheng
Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China.
Jing-Yin
JY
Mai
Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China.
Tian-Lu
TL
Hou
Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China.
Jian
J
Ping
Department of Infectious Disease, Hospital for Infectious Diseases of Pudong New Area, Shanghai 201299, P.R. China.
Jian-Jie
JJ
Chen
D055371:Acute Lung Injury / Q000097:blood / Q000188:drug therapy* / Q000473:pathology / Q000821:virology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000627:therapeutic use; D000998:Antiviral Agents / Q000737:chemistry / Q000627:therapeutic use*; D031174:Atractylodes / Q000737:chemistry; D016207:Cytokines / Q000097:blood; D009980:Influenza A virus / Q000187:drug effects*; D008168:Lung / Q000187:drug effects / Q000473:pathology / Q000821:virology; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis; D008813:Mice, Inbred ICR; D009976:Orthomyxoviridae Infections / Q000097:blood / Q000150:complications* / Q000188:drug therapy* / Q000473:pathology; D027343:Rhizome / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry / Q000627:therapeutic use*; D051199:Toll-Like Receptor 7 / Q000032:analysis
D000893:Anti-Inflammatory Agents; D000998:Antiviral Agents; D016207:Cytokines; D008562:Membrane Glycoproteins; D012717:Sesquiterpenes; C477038:Tlr7 protein, mouse; D051199:Toll-Like Receptor 7; C046196:atractylon