First report on nematode parasite infection in the yellowbar angelfish Pomacanthus maculosus (Perciformes: Pomacanthidae) from the Iraqi coral reef, with description of a new species of Cucullanus (Nematoda: Ascaridida) using the integrated approaches.
The yellowbar angelfish Pomacanthus maculosus (Forsskål) (Perciformes: Pomacanthidae) is a beautiful, marine ornamental fish with very important commercial value. However, to date, no information is available on nematode parasite infection in P. maculosus. In the present study, the integrated approaches including light and scanning electron microscopy, and sequencing and analysing ribosomal [small ribosomal DNA (18S) and internal transcribed spacer (ITS)] and mitochondrial [cytochrome c oxidase subunit 1 (cox1)] target regions, respectively, were employed for the systematic evaluation of the nematode parasites firstly isolated from P. maculosus in the Iraqi coral reef. The results revealed that these nematodes represent a new species of Cucullanus (Ascaridida: Cucullanidae). The phylogenetic analyses based on 18S, ITS and cox1 sequences were constructed, respectively, to assess the phylogenetic relationships between the new species and the other cucullanids, and the monophyly of Cucullanus, as well as some its related genus-level taxa. The results supported C. extraneus n. sp. appear to be sister to C. hainanensis, and the genera Cucullanus, Dichelyne and Truttaedacnitis may be not monophyletic assemblages. This is the first report of the occurrence of nematode parasites in P. maculosus.
Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, 050024 Shijiazhuang, Hebei Province, PR China. Electronic address: liangliangex369@126.com.
Liang
L
Li
Department of Fisheries and Marine Resources, College of Agriculture, Basrah University, Basrah, Iraq.
Atheer H
AH
Ali
Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, 050024 Shijiazhuang, Hebei Province, PR China.
Wen-Ting
WT
Zhao
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Rd., Chaoyang District, 100101 Beijing, PR China.
Liang
L
Lü
Medical College of Hebei University of Engineering, 056002 Handan, Hebei Province, PR China. Electronic address: xuzhenhm@126.com.
Zhen
Z
Xu
D000818:Animals; D017162:Ascaridida / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017191:Ascaridida Infections / Q000469:parasitology / Q000662:veterinary*; D057894:Coral Reefs; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005260:Female; D007493:Iraq; D008297:Male; D008855:Microscopy, Electron, Scanning; D058889:Molecular Typing; D010473:Perciformes / Q000469:parasitology*; D012337:RNA, Ribosomal, 18S / Q000235:genetics*
D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S; D003576:Electron Transport Complex IV
Arabian gulf; Integrated taxonomy; Ornamental fish; Parasites; Phylogeny