Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.
A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Philip J
PJ
Robinson
Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA.
Michael J
MJ
Trnka
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
David A
DA
Bushnell
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Ralph E
RE
Davis
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Pierre-Jean
PJ
Mattei
Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA.
Alma L
AL
Burlingame
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: kornberg@stanford.edu.
Roger D
RD
Kornberg
D020285:Cryoelectron Microscopy; D005786:Gene Expression Regulation; D013058:Mass Spectrometry; D056892:Mediator Complex / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D012319:RNA Polymerase II / Q000737:chemistry* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism
D056892:Mediator Complex; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D012319:RNA Polymerase II
Mediator complex; Pre-initiation complex; RNA polymerase II carboxy-terminal domain; TFIIH; TFIIK; Transcription; cross-linking; cryo-EM; mass spectrometry