Optical sensors to gain mechanistic insights into signaling assemblies.
Protein complexes play a major role in transducing information from outside the cell into instructions for growth and survival, and understanding how these complexes relay and shape intracellular signals has been a central question in signaling biology. Fluorescent proteins have proven paramount in opening windows for researchers to peer into the architecture and inner workings of signaling assemblies within the living cell and in real-time. In this review, we will provide readers with a current perspective on the development and use of genetically encoded optical probes to dissect the function of signaling complexes.
Department of Pharmacology, University of California, San Diego, La Jolla, CA, United States; Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, MD, United States.
Brian
B
Tenner
Department of Pharmacology, University of California, San Diego, La Jolla, CA, United States.
Sohum
S
Mehta
Department of Pharmacology, University of California, San Diego, La Jolla, CA, United States; Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD, United States. Electronic address: jzhang32@ucsd.edu.
Jin
J
Zhang
D015374:Biosensing Techniques / Q000379:methods*; D055620:Optical Phenomena; D015398:Signal Transduction