Identification of further diversity among posaviruses.
Recently, there have been reports of new members of posavirus-like viruses in the order Picornavirales. In this study, using a metagenomics approach, 11 posavirus-like sequences (>7,000 nucleotides) were detected in 155 porcine fecal samples. Phylogenetic analysis revealed that the newly identified virus sequences, together with other posavirus-like viruses, form distinct clusters within the order Picornavirales, composed of eight genogroups and unassigned sequences based on amino acid sequences of the helicase and RNA-dependent RNA polymerase regions, with <40 % and <50 % sequence identity, respectively. We propose further classifications of highly diverse posavirus populations based on newly identified sequences from Japanese pig feces.
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Kaori
K
Sano
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Yuki
Y
Naoi
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Mai
M
Kishimoto
Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Tottori, 683-0017, Japan.
Tsuneyuki
T
Masuda
Rokko Livestock Hygiene Service Center, Hokota, Ibaraki, 311-1593, Japan.
Hitomi
H
Tanabe
Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa, 920-3101, Japan.
Mika
M
Ito
Tochigi Prefectural South District Animal Hygiene Service Center, Tochigi, Tochigi, 328-0002, Japan.
Kazutaka
K
Niira
Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo, 208-0011, Japan.
Kei
K
Haga
Department of Bioproduction Science, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan.
Keigo
K
Asano
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Shinobu
S
Tsuchiaka
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Tsutomu
T
Omatsu
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Tetsuya
T
Furuya
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Yukie
Y
Katayama
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Mami
M
Oba
Kenhoku Livestock Hygiene Service Center, Mito, Ibaraki, 310-0002, Japan.
Yoshinao
Y
Ouchi
Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Tottori, 683-0017, Japan.
Hiroshi
H
Yamasato
Department of Bioproduction Science, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan.
Motohiko
M
Ishida
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Junsuke
J
Shirai
Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo, 208-0011, Japan.
Kazuhiko
K
Katayama
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Tetsuya
T
Mizutani
Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan. m-nagai@ishikawa-pu.ac.jp.|Department of Bioproduction Science, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan. m-nagai@ishikawa-pu.ac.jp.
Makoto
http://orcid.org/0000-0001-5884-620X
M
Nagai
D000818:Animals; D016000:Cluster Analysis; D005243:Feces / Q000821:virology*; D014644:Genetic Variation; D056186:Metagenomics; D010802:Phylogeny; D020365:RNA Helicases / Q000235:genetics; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine / Q000821:virology*
D012324:RNA-Dependent RNA Polymerase; D020365:RNA Helicases
Japan; Porcine feces; Posavirus; Sequence diversity