Single-Molecule Kinetics of Protein Adsorption on Thin Nylon-6,6 Films.
Understanding and controlling protein adsorption on surfaces is critical to a range of biological and materials applications. Kinetic details that provide the equilibrium and nonequilibrium mechanisms are difficult to acquire. In this work, single-molecule fluorescence microscopy was used to study the adsorption of Alexa 555 labeled α-lactalbumin (α-LA) on two chemically identical but morphologically different polymer surfaces: flat and porous nylon-6,6 thin films. The adsorption kinetics of spatially resolved single molecule α-LA binding to nylon films were quantified by a monolayer adsorption model. The surface morphology of the porous nylon-6,6 films increased the number of adsorption sites but decreased the binding affinity compared to the flat films. Such single-molecule based kinetic studies may be extended to various protein-polymer interactions.
Department of Chemistry, ‡Department of Electrical and Computer Engineering, and §Smalley-Curl Institute, Rice University , Houston, Texas 77251, United States.
Hao
H
Shen
Department of Chemistry, ‡Department of Electrical and Computer Engineering, and §Smalley-Curl Institute, Rice University , Houston, Texas 77251, United States.
Lawrence J
LJ
Tauzin
Department of Chemistry, ‡Department of Electrical and Computer Engineering, and §Smalley-Curl Institute, Rice University , Houston, Texas 77251, United States.
Wenxiao
W
Wang
Department of Chemistry, ‡Department of Electrical and Computer Engineering, and §Smalley-Curl Institute, Rice University , Houston, Texas 77251, United States.
Benjamin
B
Hoener
Department of Chemistry, ‡Department of Electrical and Computer Engineering, and §Smalley-Curl Institute, Rice University , Houston, Texas 77251, United States.
Bo
B
Shuang
Department of Chemistry, ‡Department of Electrical and Computer Engineering, and §Smalley-Curl Institute, Rice University , Houston, Texas 77251, United States.
Lydia
L
Kisley
Department of Chemistry, ‡Department of Electrical and Computer Engineering, and §Smalley-Curl Institute, Rice University , Houston, Texas 77251, United States.
Anneli
A
Hoggard
Department of Chemistry, ‡Department of Electrical and Computer Engineering, and §Smalley-Curl Institute, Rice University , Houston, Texas 77251, United States.
Christy F
CF
Landes
D000327:Adsorption; D005453:Fluorescence; D007700:Kinetics; D007768:Lactalbumin / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods; D009757:Nylons / Q000737:chemistry*; D016062:Porosity; D012235:Rhodamines / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry; D013499:Surface Properties; D013816:Thermodynamics
C000608607:Alexa Fluor 555; D009757:Nylons; D012235:Rhodamines; D013451:Sulfonic Acids; D007768:Lactalbumin