ER-localized adenine nucleotide transporter ER-ANT1: an integrator of energy and stress signaling in rice.
Most environmental perturbations have a direct or indirect deleterious impact on photosynthesis, and, in consequence, the overall energy status of the cell. Despite our increased understanding of convergent energy and stress signals, the connections between photosynthesis, energy and stress signals through putative common nodes are still unclear. Here we identified an endoplasmic reticulum (ER)-localized adenine nucleotide transporter1 (ER-ANT1), whose deficiency causes seedling lethality in air but viable under high CO2, exhibiting the typical photorespiratory phenotype. Metabolic analysis suggested that depletion of ER-ANT1 resulted in circadian rhythm disorders in sucrose synthesis and induced sucrose signaling pathways, indicating that the ER is involved in the regulation of vital energy metabolism in plants. In addition, the defect of ER-ANT1 triggers ER stress and activates the unfolded protein response in plant cells, suggesting ER stress and photorespiration are closely linked. These findings provide an important evidence for a key role of ER-localized ER-ANT1 in convergent energy and stress signals in rice. Our findings support the idea that ATP is a central signal involved in the plant response to a variety of stresses.
The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China. aacrav@163.com.|Guangdong Engineering Research Center of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China. aacrav@163.com.
Xiangqian
X
Zhang
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Xu
X
Zheng
The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China.
Shanwen
S
Ke
The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China.
Haitao
H
Zhu
The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China.
Fang
F
Liu
The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China.
Zemin
Z
Zhang
The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China.
Xinxiang
X
Peng
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Lin
L
Guo
The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China.
Ruizhen
R
Zeng
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Pei
P
Hou
The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China.
Ziqiang
Z
Liu
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Suowei
S
Wu
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Meifang
M
Song
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. yangjianping02@caas.cn.
Jianping
J
Yang
The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China. gqzhang@scau.edu.cn.
Guiquan
G
Zhang
D004721:Endoplasmic Reticulum / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D012275:Oryza / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction; D056811:Unfolded Protein Response / Q000502:physiology
ATP transporter; ER-ANT1; Endoplasmic reticulum; Photorespiration; Rice; Stress signaling