Flavonoids from Caragana pruinosa roots.
A new pterocarpan derivative, pruinosanone D (1), a new isoflavonoid, pruinosanone E (2), and a new chalcone, pruinosanone F (3), were isolated from Caragana pruinosa roots, along with four known analogues (4-7), identified as 2,4-dihydroxy-3'-methoxy-4'-ethoxychalcone, 7,4-dihydroxyflavanone, butin and scutellaprostin C, respectively. Their structures were elucidated by detailed analyses of NMR, IR, and MS data. The ability of the isolated compounds to prevent nitric oxide (NO) production by LPS-stimulated RAW 264.7 macrophages was also studied. Compound 1 were among the most potent NO production inhibitor, with IC50value of 0.62μM.
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China; College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
Wei
W
Peng
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
Liang
L
Wang
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
Xu-Hui
XH
Qiu
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
Yi-Ping
YP
Jiang
Xinjiang Institute of Chinese Materia Medica and Ethnodrug, Urumqi 830002, China.
Lan
L
Pan
Xinjiang Institute of Chinese Materia Medica and Ethnodrug, Urumqi 830002, China.
Xiao-Guang
XG
Jia
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China. Electronic address: qinsmmu@126.com.
Lu-Ping
LP
Qin
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China. Electronic address: zhengchengjian1984@126.com.
Cheng-Jian
CJ
Zheng
D000818:Animals; D032602:Caragana / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D018517:Plant Roots / Q000737:chemistry*; D036343:Pterocarpans / Q000737:chemistry* / Q000302:isolation & purification; D000067996:RAW 264.7 Cells
D005419:Flavonoids; D036343:Pterocarpans; C000613548:pruinosanone D; D009569:Nitric Oxide
Caragana pruinosa; Flavonoids; Nitric oxide; Pruinosanones