An OGA-Resistant Probe Allows Specific Visualization and Accurate Identification of O-GlcNAc-Modified Proteins in Cells.
O-linked β-N-acetyl-glucosamine (O-GlcNAc) is an essential and ubiquitous post-translational modification present in nucleic and cytoplasmic proteins of multicellular eukaryotes. The metabolic chemical probes such as GlcNAc or GalNAc analogues bearing ketone or azide handles, in conjunction with bioorthogonal reactions, provide a powerful approach for detecting and identifying this modification. However, these chemical probes either enter multiple glycosylation pathways or have low labeling efficiency. Therefore, selective and potent probes are needed to assess this modification. We report here the development of a novel probe, 1,3,6-tri-O-acetyl-2-azidoacetamido-2,4-dideoxy-d-glucopyranose (Ac34dGlcNAz), that can be processed by the GalNAc salvage pathway and transferred by O-GlcNAc transferase (OGT) to O-GlcNAc proteins. Due to the absence of a hydroxyl group at C4, this probe is less incorporated into α/β 4-GlcNAc or GalNAc containing glycoconjugates. Furthermore, the O-4dGlcNAz modification was resistant to the hydrolysis of O-GlcNAcase (OGA), which greatly enhanced the efficiency of incorporation for O-GlcNAcylation. Combined with a click reaction, Ac34dGlcNAz allowed the selective visualization of O-GlcNAc in cells and accurate identification of O-GlcNAc-modified proteins with LC-MS/MS. This probe represents a more potent and selective tool in tracking, capturing, and identifying O-GlcNAc-modified proteins in cells and cell lysates.
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University , Haihe Education Park, 38 Tongyan Road, Tianjin 300353, People's Republic of China.|Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University , 50 Decatur St SE, Atlanta, Georgia 30303, United States.
Jing
J
Li
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University , Haihe Education Park, 38 Tongyan Road, Tianjin 300353, People's Republic of China.|School of Food and Drug, Luoyang Normal University , Luoyang, People's Republic of China.
Jiajia
J
Wang
Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University , 50 Decatur St SE, Atlanta, Georgia 30303, United States.
Liuqing
L
Wen
Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University , 50 Decatur St SE, Atlanta, Georgia 30303, United States.
He
H
Zhu
Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University , 50 Decatur St SE, Atlanta, Georgia 30303, United States.
Shanshan
S
Li
Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University , 50 Decatur St SE, Atlanta, Georgia 30303, United States.
Kenneth
K
Huang
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University , Haihe Education Park, 38 Tongyan Road, Tianjin 300353, People's Republic of China.
Kuan
K
Jiang
Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University , 50 Decatur St SE, Atlanta, Georgia 30303, United States.
Xu
X
Li
Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University , 50 Decatur St SE, Atlanta, Georgia 30303, United States.
Cheng
C
Ma
Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University , 50 Decatur St SE, Atlanta, Georgia 30303, United States.
Jingyao
J
Qu
Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University , 50 Decatur St SE, Atlanta, Georgia 30303, United States.
Aishwarya
A
Parameswaran
Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University , 50 Decatur St SE, Atlanta, Georgia 30303, United States.
Jing
J
Song
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University , Haihe Education Park, 38 Tongyan Road, Tianjin 300353, People's Republic of China.
Wei
W
Zhao
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University , Haihe Education Park, 38 Tongyan Road, Tianjin 300353, People's Republic of China.|Department of Chemistry and Center of Diagnostics & Therapeutics, Georgia State University , 50 Decatur St SE, Atlanta, Georgia 30303, United States.
Peng George
PG
Wang
D000117:Acetylglucosamine / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D006801:Humans; D051379:Mice; D015335:Molecular Probes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
D015335:Molecular Probes; D011506:Proteins; D000117:Acetylglucosamine