Development of a SNP array and its application to genetic mapping and diversity assessment in pepper (Capsicum spp.).
The development and application of single nucleotide polymorphisms (SNPs) is in its infancy for pepper. Here, a set of 15,000 SNPs were chosen from the resequencing data to develop an array for pepper with 12,720 loci being ultimately synthesized. Of these, 8,199 (~64.46%) SNPs were found to be scorable and covered ~81.18% of the whole genome. With this array, a high-density interspecific genetic map with 5,569 SNPs was constructed using 297 F2 individuals, and genetic diversity of a panel of 399 pepper elite/landrace lines was successfully characterized. Based on the genetic map, one major QTL, named Up12.1, was detected for the fruit orientation trait. A total of 65 protein-coding genes were predicted within this QTL region based on the current annotation of the Zunla-1 genome. In summary, the thousands of well-validated SNP markers, high-density genetic map and genetic diversity information will be useful for molecular genetics and innovative breeding in pepper. Furthermore, the mapping results lay foundation for isolating the genes underlying variation in fruit orientation of Capsicum.
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Jiaowen
J
Cheng
Pepper Institute, Zunyi Academy of Agricultural Sciences, Zunyi, Guizhou 563102, China.|Guizhou Provincial College-based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines, Zunyi Medical University, Zunyi, Guizhou 563000, China.
Cheng
C
Qin
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Xin
X
Tang
Guangzhou Genedenovo Biotechnology Co., Ltd, Guangzhou 510006, China.
Huangkai
H
Zhou
BGI-Shenzhen, Shenzhen 518083, China.
Yafei
Y
Hu
Department of Computer Science, City University of Hong Kong, Hong Kong 999077, China.
Zicheng
Z
Zhao
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Junjie
J
Cui
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Bo
B
Li
College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Zhiming
Z
Wu
Pepper Institute, Zunyi Academy of Agricultural Sciences, Zunyi, Guizhou 563102, China.
Jiping
J
Yu
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Kailin
K
Hu
D001947:Breeding; D002212:Capsicum / Q000235:genetics*; D002874:Chromosome Mapping; D020224:Expressed Sequence Tags; D005838:Genotype; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*