[Overexpression of KIAA1456 inhibits the proliferation of HO8910PM cells].
Objective To investigate the effect of over-expressed KIAA1456 on the proliferation and cell cycle of HO8910PM cells. Methods KIAA1456 was amplified by PCR and inserted into lentiviral expression vector Ubi-MCS-EGFP-IRES-puromycin to construct Ubi-KIAA1456-EGFP-puromycin (LV-KIAA1456) vector. Thereafter, the lentiviral particles were packaged and used to infect HO8910PM cells. The green fluorescence protein (GFP) expression was observed at 72 hours. The expression of KIAA1456 was examined by reverse transcription PCR and Western blotting. Cell growth was detected with CCK-8 assay and cell cycles were analyzed by flow cytometry. Results Reverse transcription PCR and Western blotting showed that HO8910PM cells infected with LV-KIAA1456 vector over-expressed KIAA1456 mRNA and protein. Cell proliferation was inhibited and G1 phase cells significantly increased in HO8910PM cells overexpressing KIAA1456. Conclusion Over-expression of KIAA1456 inhibits the proliferation of HO8910PM ovarian cancer cells and arrests the cell cycle in G1 phase.
Department of Obstetrics and Gynecology, University-Town Hospital, Chongqing Medical University, Chongqing 401331, China.
Huaimei
H
Chen
Department of Obstetrics and Gynecology, University-Town Hospital, Chongqing Medical University, Chongqing 401331, China. *Corresponding author, E-mail: 752203031@qq.com.
Jia
J
Wang
Department of Obstetrics and Gynecology, University-Town Hospital, Chongqing Medical University, Chongqing 401331, China.
Yingfeng
Y
Zhang
Department of Obstetrics and Gynecology, University-Town Hospital, Chongqing Medical University, Chongqing 401331, China.
Yanhong
Y
Gao
D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology*; D014162:Transfection; D012359:tRNA Methyltransferases / Q000235:genetics* / Q000378:metabolism
C582289:TRMT9B protein, human; D012359:tRNA Methyltransferases