Multigenerational Collision-Induced Dissociation for Characterization of Organic Compounds.
There are many cases in which limited information is obtained from a single stage of tandem mass spectrometry (MS2or MS/MS). For example, isomeric cathinones give similar product ion MS2spectra, but they can be differentiated by their unique MS3fragments. Other drugs such as oxycodone and noroxycodone lose water in a single-stage tandem mass spectrometry experiment but give rich structural information in subsequent stages, as do many peptides. Here we utilize multigenerational collision-induced dissociation (CID) on a miniature mass spectrometer and emphasize useful applications. Unique fragmentation patterns consisting of several generations of fragment ions are obtained in these multigenerational spectra, allowing discrimination of cathinone isomers and structural characterization of small molecules, including drugs and peptides, all using a single sequence of ion injection, isolation, and mass scanning.
Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University , West Lafayette, Indiana 47907, United States.
Dalton T
DT
Snyder
Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University , West Lafayette, Indiana 47907, United States.
Patrick W
PW
Fedick
Department of Chemistry and Center for Analytical Instrumentation Development, Purdue University , West Lafayette, Indiana 47907, United States.
R Graham
RG
Cooks