Effective Lactobacillus plantarum and Bifidobacterium infantis encapsulation with chia seed (Salvia hispanica L.) and flaxseed (Linum usitatissimum L.) mucilage and soluble protein by spray drying.
Mucilage (M) and soluble protein (SP) extracted from chia seed and flaxseed were used as encapsulating material for two probiotic bacteria: Bifidobacterium infantis and Lactobacillus plantarum by spray drying. Probiotic survival and viability after spray drying and during storage were evaluated. B. infantis and L. plantarum displayed high survival (⩾98%) after encapsulation with mixtures of maltodextrin (MD) combined with M and SP from flaxseed (MD:FM:FSP - 7.5:0.2:7.5%, w/w/w) and chia seed (MD:CM:CSP - 7.5:0.6:7.5%, w/w/w), respectively. These ternary blends protected the probiotics and enhanced their resistance to simulated gastric juice and bile solution. Probiotics encapsulated with the ternary blends incorporated in instant juice powder exhibited high viability (>9Log10CFU/g) after 45days refrigerated storage. Encapsulation with the ternary blends reduced particle size of the probiotic powders thereby offering additional functional benefits. Our results reveal that chia seed and flaxseed are excellent sources of probiotic encapsulating agents.
Department of Chemical Engineering, Scientific and Technological Bioresource Nucleus, BIOREN, Universidad de La Frontera, Casilla 54-D, Temuco, Chile. Electronic address: mariela.bustamante@ufrontera.cl.
Mariela
M
Bustamante
National Bioproducts and Bioprocesses Program, Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, BC V0H 1Z0, Canada.
B Dave
BD
Oomah
Department of Chemical Engineering, Scientific and Technological Bioresource Nucleus, BIOREN, Universidad de La Frontera, Casilla 54-D, Temuco, Chile.
Mónica
M
Rubilar
Department of Chemical Engineering, Scientific and Technological Bioresource Nucleus, BIOREN, Universidad de La Frontera, Casilla 54-D, Temuco, Chile; Centre for Biotechnology and Bioengineering (CeBiB), Universidad de La Frontera, Casilla 54-D, Temuco, Chile.
Carolina
C
Shene
D000070236:Bifidobacterium longum subspecies infantis / Q000187:drug effects / Q000254:growth & development*; D003890:Desiccation; D019597:Flax / Q000737:chemistry*; D005612:Freeze Drying; D005750:Gastric Juice / Q000187:drug effects / Q000382:microbiology; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000254:growth & development*; D053147:Plant Exudates / Q000737:chemistry*; D011208:Powders / Q000737:chemistry*; D019936:Probiotics / Q000008:administration & dosage*; D027544:Salvia / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
D053147:Plant Exudates; D011208:Powders
Chia seeds; Flaxseed; Microencapsulation; Probiotics; Spray drying