Oxidative stress of crystalline lens in rat menopausal model.
OBJECTIVE
To evaluate lenticular oxidative stress in rat menopausal models.


METHODS
Forty Wistar female albino rats were included in this study. A total of thirty rats underwent oophorectomy to generate a menopausal model. Ten rats that did not undergo oophorectomy formed the control group (Group 1). From the rats that underwent oophorectomy, 10 formed the menopause control group (Group 2), 10 were administered a daily injection of methylprednisolone until the end of the study (Group 3), and the remaining 10 rats were administered intraperitoneal streptozocin to induce diabetes mellitus (Group 4). Total oxidant status (TOS), total antioxidant capacity (TAC), and oxidative stress index (OSI) measurements of the crystalline lenses were analyzed.


RESULTS
The mean OSI was the lowest in group 1 and highest in group 4. Nevertheless, the difference between the groups was not statistically significant in terms of OSI (p >0.05). The mean TOS values were similar between the groups (p >0.05), whereas the mean TAC of group 1 was significantly higher than that of the other groups (p <0.001).


CONCLUSIONS
Our results indicate that menopause may not promote cataract formation.
Department of Ophthalmology, Pamukkale University, Denizli, Turkey.
Semra
S
Acer
Department of Ophthalmology, Pamukkale University, Denizli, Turkey.
Gökhan
G
Pekel
Department of Physiology, Pamukkale University, Denizli, Turkey.
Vural
V
Küçükatay
Department of Gynecology and Obstetrics, Pamukkale University, Denizli, Turkey.
Aysun
A
Karabulut
Department of Ophthalmology, Pamukkale University, Denizli, Turkey.
Ramazan
R
Yağcı
Department of Ophthalmology, Pamukkale University, Denizli, Turkey.
Ebru Nevin
EN
Çetin
Department of Anatomy, Pamukkale University, Denizli, Turkey.
Şahika Pınar
ŞP
Akyer
Experimental Animals Unit, Pamukkale University, Denizli, Turkey.
Barbaros
B
Şahin
D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D002386:Cataract / Q000209:etiology / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D008593:Menopause / Q000378:metabolism*; D008775:Methylprednisolone / Q000494:pharmacology; D023421:Models, Animal; D010052:Ovariectomy; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D017208:Rats, Wistar; D012016:Reference Values; D013053:Spectrophotometry
D000975:Antioxidants; D005938:Glucocorticoids; D016877:Oxidants; D008775:Methylprednisolone