Study on binding and fluorescence energy transfer efficiency of Rhodamine B with Pluronic F127-gold nanohybrid using optical spectroscopy methods.
This work focuses on the binding efficiency and fluorescence resonance energy transfer (FRET) of fluorescent dye Rhodamine B (Rh B) to Pluronic F127-gold nanohybrid. The formation of gold nanoparticles inside Rh B doped Pluronic F127 copolymer have been characterized using dynamic light scattering study, HR-TEM images, UV-visible spectra and fluorescence studies. Fluorescence quenching and the constant fluorescence lifetime of the Rhodamine B present in the cavity of Pluronic F127-gold nanohybrid suggested a strong binding ability (3.5×103Lmol-1), static nature of quenching and better energy transfer efficiency of fluorescent dye towards Pluronic F127-gold (Au) nanohybrids.
Department of Chemistry, Annamalai University, Chidambaram 608002, India.
Jenif Dsouza
JD
Antonisamy
Department of Chemistry, Indian Institute of Technology Madras, Chennai 600 036, India.
Jitendriya
J
Swain
Department of Chemistry, Annamalai University, Chidambaram 608002, India. Electronic address: mishra342sas@gmail.com.
Sasmita
S
Dash
Fluorescence spectroscopy; Gold nanoparticles; Micelles; Pluronic F127 copolymer; Rhodamine B