Optimization of polyhydroxybutyrate production utilizing waste water as nutrient source by Botryococcus braunii Kütz using response surface methodology.
Investigations have been made to optimize various factors including pH, temperature, and substrate for enhanced polyhydroxybutyrate (PHB) production in Botryococcus braunii which serves as a pioneer for production of bioplastic (PHB). Polyhydroxybutyrate is a natural, decomposable polymers accumulated by the microorganism under different nutritional condition. Strain selection was done by staining method using Sudan black and Nile red dye. Using response surface methodology (RSM), three level- three variables Box Behnken design (BBD), the best potential combination of pH (4-11), temperature (30-50°C) and sewage waste water as substrate fed at different concentrations at 20%-100% for maximum PHB production was investigated. Maximum yield (247±0.42mg/L) of PHB dry weight was achieved from the 60% concentration of sewage waste water as a growth medium at pH 7.5 at 40°C. It was well in close agreement with the value predicted by RSM model yield (246± 0.32mg/L). Thus the study shows the production of PHB by B. braunii along with the basic characterization of PHB by using FTIR and TEM analysis. These preliminary studies indicated that PHB can also be produced by B. braunii utilizing waste water. There is no report on the optimization of PHB production in this microalgae have been documented.
Centre for Advanced Studies in Botany, University of Madras, Guindy campus, Chennai 600 025, Tamil Nadu, India. Electronic address: algalkavi@gmail.com.
Ganapathy
G
Kavitha
Centre for Advanced Studies in Botany, University of Madras, Guindy campus, Chennai 600 025, Tamil Nadu, India.
Chidambaram
C
Kurinjimalar
Centre for Advanced Studies in Botany, University of Madras, Guindy campus, Chennai 600 025, Tamil Nadu, India.
Krishnan
K
Sivakumar
Centre for Advanced Studies in Botany, University of Madras, Guindy campus, Chennai 600 025, Tamil Nadu, India.
Muthukumar
M
Kaarthik
Centre for Advanced Studies in Botany, University of Madras, Guindy campus, Chennai 600 025, Tamil Nadu, India.
Rajamani
R
Aravind
Centre for Advanced Studies in Botany, University of Madras, Guindy campus, Chennai 600 025, Tamil Nadu, India.
Perumal
P
Palani
Centre for Advanced Studies in Botany, University of Madras, Guindy campus, Chennai 600 025, Tamil Nadu, India.
Ramasamy
R
Rengasamy
D006863:Hydrogen-Ion Concentration; D058086:Microalgae / Q000378:metabolism*; D008962:Models, Theoretical; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*; D013696:Temperature
D011091:Polyesters; D012722:Sewage; C107955:poly(4-hydroxybutanoate)
Fourier transmission infrared spectroscopy (FTIR); Poly hydroxyl butyrate (PHB); Response surface methodology (RSM); Transmission electron microscope (TEM)