MiR-21 inhibitor suppressed the progression of retinoblastoma via the modulation of PTEN/PI3K/AKT pathway.
MicroRNA-21 (miR-21) was reported to act as an oncogene during the development of many human tumors. However, little was revealed about the function of miR-21 in retinoblastoma (RB). In this study, we examined the expression of miR-21 in RB tissues and explored the relationship between miR-21 and phosphatase and tensin homolog (PTEN)/phosphatidylinositol-3-OH kinase (PI3K)/AKT signal. Quantitative real-time PCR (qRT-PCR) results showed that the level of miR-21 in RB tissues was higher than that in retinal normal tissues. In Weri-Rb-1 cells, miR-21 inhibitor suppressed the expression of miR-21 and cell viability, but improved cell apoptotic rates by modulating the levels of PDCD4, Bax, and Bcl-2. Meanwhile, miR-21 inhibitor suppressed cell migration and invasion via inhibiting the protein levels of MMP2 and MMP9 and significantly affected the expression of PTEN, PI3K, and p-AKT. Taken together, miR-21 inhibitor suppressed cell proliferation, migration, and invasion via the PTEN/PI3K/AKT signal. These findings revealed the molecular basis of miR-21 functioning in the progression of RB and provided a new means for cell therapy in RB.
Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, China.
Fu
F
Gui
Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, China.
Zhengdong
Z
Hong
Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, China.
Zhipeng
Z
You
Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, China.
Hongxi
H
Wu
Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, China.
Yulan
Y
Zhang
D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007223:Infant; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D012175:Retinoblastoma / Q000235:genetics* / Q000473:pathology*; D015398:Signal Transduction
C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
PTEN/PI3K/AKT signal; invasion; miRNA-21; migration; proliferation; retinoblastoma