Reuse of E-plate cell sensor arrays in the xCELLigence Real-Time Cell Analyzer.
The xCELLigence Real-Time Cell Analyzer (RTCA) is a non-invasive, impedence-based biosensor system that can measure cell viability, migration, growth, spreading, and proliferation. Changes in cell morphology and behavior are continuously monitored in real time using microelectronics located in the wells of RTCA E-plates. According to the manufacturer's recommendation, E-plates are single-use and disposable. Here, we show that E-plates can be regenerated and reused several times without significantly effecting experimental results.
Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Gdansk, Poland.
Justyna
J
Stefanowicz-Hajduk
Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Gdansk, Poland.
Anna
A
Adamska
Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Gdansk, Poland.
Rafal
R
Bartoszewski
Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Gdansk, Poland.
J Renata
JR
Ochocka
D015374:Biosensing Techniques / Q000379:methods*; D048430:Cell Shape / Q000502:physiology; D003584:Cytological Techniques / Q000379:methods*; D017097:Electric Impedance; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D017743:Equipment Reuse; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells
RTCA system; cell index; impedance; regeneration; sensor-electrodes; trypsinization