Recent Advances and Future Direction in Lyophilisation and Desiccation of Mesenchymal Stem Cells.
Mesenchymal Stem Cells (MSCs) are a promising mammalian cell type as they can be used for the reconstruction of human tissues and organs. MSCs are shown to form bone, cartilage, fat, and muscle-like cells under specific cultivation conditions. Current technology of MSCs cryopreservation has significant disadvantages. Alternative technologies of mammalian cells preservation through lyophilisation or desiccation (air-drying) are among the upcoming domains of investigation in the field of cryobiology. Different protectants and their combinations were studied in this context. Loading of the protectant in the live cell can be a challenging issue but recent studies have shown encouraging results. This paper deals with a review of the protectants, methods of their delivery, and physical boundary conditions adopted for the desiccation and lyophilisation of mammalian cells, including MSCs. A hybrid technique combining both methods is also proposed as a promising way of MSCs dry preservation.
Department of Biomedical Engineering, National Institute of Technology, Raipur 492010, India.
Akalabya
A
Bissoyi
Department of Biotechnology, National Institute of Technology, Raipur 492010, India.
Awanish
0000-0001-8735-479X
A
Kumar
Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420000, Russia.
Albert A
0000-0002-9427-5739
AA
Rizvanov
Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420000, Russia.
Alexander
0000-0003-4686-2303
A
Nesmelov
Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420000, Russia; Preventive Medicine & Diagnosis Innovation Program (PMI), Division of Genomic Technologies, RIKEN, Yokohama 2300045, Japan.
Oleg
O
Gusev
Department of Biochemistry, Pt. JNM Medical College, Raipur 492001, India.
Pradeep Kumar
PK
Patra
Department of Biomedical Engineering, National Institute of Technology, Raipur 492010, India.
Arindam
0000-0003-4738-8324
A
Bit