Colloidal gold-McAb probe-based rapid immunoassay strip for simultaneous detection of fumonisins in maize.
BACKGROUND
Fumonisins are a kind of toxic and carcinogenic mycotoxin. A rapid immunochromatographic test strip has been developed for simultaneous detection of fumonisin B1, B2and B3(FB1, FB2and FB3) in maize based on colloidal gold-labelled monoclonal antibody (McAb) against FB1probe.


RESULTS
The anti-FB1McAb (2E11-H3) was produced through immunisation and cell fusion, and identified as high affinity, specificity and sensitivity. The cross-reaction ratios with fumonisin B2and B3were accordingly 385% and 72.4%, while none with other analogues. The colloid gold-labelled anti-FB1McAb probe was successfully prepared and used for establishing the immunochromatographic strip. The test strip showed high sensitivity and specificity, the IC50for FB1was 58.08 ng mL-1, LOD was 11.24 ng mL-1, calculated from standard curve. Moreover, the test strip exhibited high cross-reactivity with FB2and FB3, and could be applied to the simultaneous detection of FBs (FB1:FB2:FB3= 12:4:1) in maize sample with high accuracy and precision. The average recoveries of FBs in maize ranged from 90.42% to 95.29%, and CVs were 1.25-3.77%. The results of the test strip for FBs samples showed good correlation with high-performance liquid chromatography analysis.


CONCLUSIONS
The immunochromatographic test strip could be employed in the rapid simultaneous detection of FB1, FB2and FB3in maize samples on-site. © 2016 Society of Chemical Industry.
Henan Academy of Agriculture Science/Key Laboratory of Animal Immunology, Ministry of Agriculture/Henan key Laboratory of Animal Immunology, Zhengzhou, 450002, China.
Jingjing
J
Yao
Henan Academy of Agriculture Science/Key Laboratory of Animal Immunology, Ministry of Agriculture/Henan key Laboratory of Animal Immunology, Zhengzhou, 450002, China.
Yaning
Y
Sun
Henan Academy of Agriculture Science/Key Laboratory of Animal Immunology, Ministry of Agriculture/Henan key Laboratory of Animal Immunology, Zhengzhou, 450002, China.
Qingmei
Q
Li
Henan Academy of Agriculture Science/Key Laboratory of Animal Immunology, Ministry of Agriculture/Henan key Laboratory of Animal Immunology, Zhengzhou, 450002, China.
Fangyu
F
Wang
Henan Academy of Agriculture Science/Key Laboratory of Animal Immunology, Ministry of Agriculture/Henan key Laboratory of Animal Immunology, Zhengzhou, 450002, China.
Man
M
Teng
Henan Academy of Agriculture Science/Key Laboratory of Animal Immunology, Ministry of Agriculture/Henan key Laboratory of Animal Immunology, Zhengzhou, 450002, China.
Yanyan
Y
Yang
Henan Academy of Agriculture Science/Key Laboratory of Animal Immunology, Ministry of Agriculture/Henan key Laboratory of Animal Immunology, Zhengzhou, 450002, China.
Ruiguang
R
Deng
Henan Academy of Agriculture Science/Key Laboratory of Animal Immunology, Ministry of Agriculture/Henan key Laboratory of Animal Immunology, Zhengzhou, 450002, China.
Xiaofei
X
Hu
D000911:Antibodies, Monoclonal / Q000032:analysis; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D037341:Fumonisins / Q000032:analysis*; D017979:Gold Colloid / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D037341:Fumonisins; D017979:Gold Colloid; C056934:fumonisin B2; C056933:fumonisin B1
colloidal gold; fumonisins; immunochromatographic test strip; monoclonal antibody