Monitoring of bacterial contamination of dental unit water lines using adenosine triphosphate bioluminescence.
Bacterial contamination of dental unit waterlines (DUWLs) was evaluated using ATP bioluminescence analysis and a conventional culture method. Water samples (N=44) from DUWLs were investigated for heterotrophic bacteria by culture on R2A agar, which gave counts ranging from 1.4×103to 2.7×105cfu/mL. The ATP bioluminescence results for DUWL samples ranged from 6 to 1189 relative light units and could be obtained within 1min; these correlated well with the culture results (r=0.727-0.855). We conclude that the results of the ATP bioluminescence assay accurately reflect the results of conventional culture-based testing. This method is potentially useful for rapid and simple monitoring of DUWL bacterial contamination.
Department of Oral Health Care and Rehabilitation, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan. Electronic address: akari.watanabe@tokushima-u.ac.jp.
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Watanabe
Department of Preventive Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
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Tamaki
Graduate School of Health Sciences, Okayama University, Okayama, Japan.
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Yokota
Department of Oral Health Care and Rehabilitation, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
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Matsuyama
Department of Oral Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
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Kokeguchi
D000255:Adenosine Triphosphate / Q000032:analysis*; D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D015169:Colony Count, Microbial; D003770:Dental Offices; D004203:Disinfection / Q000379:methods*; D006801:Humans; D008163:Luminescent Measurements; D014871:Water Microbiology
D000255:Adenosine Triphosphate
ATP bioluminescence; Bacteria; Contamination; Dental unit water line; Infection control