Neuron-astrocyte interactions in spinal cord dorsal horn in neuropathic pain development and docosahexaenoic acid therapy.
The analgesic activity of docosahexaenoic acid (DHA, 22:6 n-3) was studied using a chronic constriction injury (CCI) rat model. Animals were subcutaneously injected with DHA emulsion at a dose of 4.5mg/kg (125mМ/kg) daily during 2weeks after surgery. We characterized the dynamics of GFAP-positive astrocyte, substance P (SP) and nNOS-positive neurons activity in the spinal cord dorsal horn (SCDH) superficial lamina. We found that DHA treatment decrease the intensity and duration of neurogenic pain syndrome, results in earlier stabilization of weight distribution, prevents the cold allodynia and dystrophic changings in denervated limb tissue. DHA treatment reduced the reactive astrocyte number, decrease SP-immunopositive fibers and nNOS-positive neurons number in the SCDH in neuropathic pain.
A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 17 Palchevskii Str., 690041, Russia; School of Biomedicine, Far Eastern Federal University, Vladivostok, 8 Sukhanova Str., 690950, Russia. Electronic address: i-manzhulo@bk.ru.
Igor V
IV
Manzhulo
A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 17 Palchevskii Str., 690041, Russia.
Olga S
OS
Ogurtsova
A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 17 Palchevskii Str., 690041, Russia.
Yuliya O
YO
Kipryushina
A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 17 Palchevskii Str., 690041, Russia.
Nikolay A
NA
Latyshev
A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 17 Palchevskii Str., 690041, Russia.
Sergey P
SP
Kasyanov
A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 17 Palchevskii Str., 690041, Russia.
Inessa V
IV
Dyuizen
A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 17 Palchevskii Str., 690041, Russia; School of Biomedicine, Far Eastern Federal University, Vladivostok, 8 Sukhanova Str., 690950, Russia.
Anna A
AA
Tyrtyshnaia
D000700:Analgesics / Q000627:therapeutic use*; D000704:Analysis of Variance; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D001253:Astrocytes / Q000502:physiology*; D004008:Diclofenac / Q000627:therapeutic use; D004195:Disease Models, Animal; D004281:Docosahexaenoic Acids / Q000627:therapeutic use*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006930:Hyperalgesia / Q000175:diagnosis / Q000503:physiopathology; D008297:Male; D051379:Mice; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012585:Sciatica / Q000188:drug therapy* / Q000473:pathology* / Q000503:physiopathology; D066148:Spinal Cord Dorsal Horn / Q000502:physiology*; D013373:Substance P / Q000378:metabolism; D013997:Time Factors; D016474:Weight-Bearing / Q000502:physiology*
D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D005904:Glial Fibrillary Acidic Protein; D004008:Diclofenac; D004281:Docosahexaenoic Acids; D013373:Substance P; D010751:Phosphopyruvate Hydratase
Astrocyte; Chronic constriction injury; Docosahexaenoic acid; Nitric oxide synthase; Spinal cord dorsal horn; Substance P