APOBEC3G governs the generation of truncated AATF protein to ensure oncogenic transformation.
The oncogenic potential of Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G (APOBEC3G) was recently appreciated by the finding that revealed its ability to downregulate Kruppel-like factor 4 (KLF4) gene translation through its affinity for 3'UTR of KLF4 mRNA. Keeping in view the fact that KLF4 is known to repress apoptosis antagonizing transcription factor (AATF) gene expression, the present study employed stem cells as archetype model to explore the effect of APOBEC3G over-expression upon AATF gene expression within these cells as well as on the genes involved in oncogenic transformation. Such a study revealed that APOBEC3G had the ability to bind AATF mRNA within its third exon to facilitate the generation of truncated 23 kDa AATF translation product which, in turn, had the inherent capacity to be the crucial mediator of APOBEC3G induced oncogenic transformation within such cells.
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
Shaveta
S
Sharma
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
Anuradha
A
Garg
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
Rakesh S
RS
Dhanda
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
Deepak
D
Kaul
D000071480:APOBEC-3G Deaminase / Q000235:genetics / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D019295:Computational Biology; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D000090062:Kruppel-Like Factor 4; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D050505:Mutant Proteins / Q000378:metabolism*; D011485:Protein Binding; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism; D000097584:DNA Methyltransferase 3B
D000071480:APOBEC-3G Deaminase; D051017:Apoptosis Regulatory Proteins; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090062:Kruppel-Like Factor 4; D050505:Mutant Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D000097584:DNA Methyltransferase 3B; C402637:AATF protein, human; C000715467:KLF4 protein, human; C464924:APOBEC3G protein, human
APOBEC3G; CD34+ stem cells; oncogenes; truncated-AATF