Heterotrophic cultivation of Chlorella pyrenoidosa using sucrose as the sole carbon source by co-culture with Rhodotorula glutinis.
Heterotrophic cultivation of microalgae is a feasible alternative strategy to avoid the light limitation of photoautotrophic culture, but the heterotrophic utilization of disaccharides is difficult for microalgae. Aimed at this problem, a co-culture system was developed by mix culture of C. pyrenoidosa and R. glutinis using sucrose as the sole carbon source. In this system, C. pyrenoidosa could utilize glucose and fructose which were hydrolyzed from sucrose by R. glutinis. The highest specific growth rate and final cell number proportion of algae was 1.02day(-1) and 45%, respectively, when cultured at the initial algal cell number proportion of 95.24% and the final algal cell density was 111.48×10(6)cells/mL. In addition, the lipid content was also promoted due to the synergistic effects in mix culture. This study provides a novel approach using sucrose-riched wastes for the heterotrophic culture of microalgae and may effectively decrease the cost of carbon source.
Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, PR China. Electronic address: skwang@yzu.edu.cn.
Shikai
S
Wang
Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, PR China.
Yong
Y
Wu
Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, PR China.
Xu
X
Wang
D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology*; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development*; D018920:Coculture Techniques / Q000379:methods*; D052836:Heterotrophic Processes / Q000187:drug effects; D008055:Lipids / Q000032:analysis; D012248:Rhodotorula / Q000187:drug effects / Q000254:growth & development*; D013395:Sucrose / Q000494:pharmacology*
D008055:Lipids; D013395:Sucrose; D002244:Carbon
Biomass; Chlorella pyrenoidosa; Co-culture; Lipid; Rhodotorula glutinis; Sucrose