[Elevated expression of B7-H6 in U87 cells-derived glioma stem like cells is associated with biological characteristics].
Objective To investigate the expression and biological significance of costimulatory molecule B7-H6, a member of B7 family, in glioma stem like cells (GSLCs). Methods In virtue of the ability of forming neurospheres in vitro , GSLCs were isolated from U87 cells by cell sub-cloning. Real-time quantitative PCR and flow cytometry were performed to detect the expressions of stem cell related markers (c-myc, Sox2, CD133, nestin, and CXCR4), as well as the expressions of B7 family molecules. The different doses of adriamycin, carboplatin, cisplatin, were used to treat GSLCs for testing their chemotherapy-resistance. After the expression of B7-H6 in GSLCs was knockdown by siRNA, CCK-8 method was used to detect cell proliferation. Results GSLCs were successfully isolated from U87 cells, which formed neurospheres in vitro . The expressions of multiple stem cell markers were up-regulated and the GSLCs showed enhanced chemo therapy-resistance. B7 family members, B7-H1, B7-H3, B7-H4 and B7-H6 were expressed in GSLCs. Compared with primary U87 cells, GSLCs presented with a remarkably increased expression of B7-H6 on cell membrane. When B7-H6 was silenced by siRNA, cell proliferation was inhibited along with the decrease of c-myc expression. Conclusion The expression of B7-H6 is up-regulated in U87-derived GSLCs, which is associated with the biological characteristics of GSLCs.
Department of Immunology, School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215000, China.
Hanqing
H
Chen
Department of Immunology, School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215000, China.
Zhengpeng
Z
Shi
Department of Immunology, School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215000, China.
Bing
B
Gao
Jiangsu Institute of Clinical Immunology, First Affiliated Hospital, Soochow University, Suzhou 215000, China. *Corresponding authors, E-mail: xueguangzh@126.com.
Fengqing
F
Fu
Jiangsu Institute of Clinical Immunology, First Affiliated Hospital, Soochow University, Jiangsu Provincial Key Laboratory of Stem Cell and Biomedical Materials, Soochow University, Suzhou 215000, China. *Corresponding authors, E-mail: fufengqing@suda.edu.cn.
Xueguang
X
Zhang
D060887:B7 Antigens / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D006801:Humans; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
D060887:B7 Antigens; C527276:CXCR4 protein, human; D019718:Receptors, CXCR4