GUIDE-Seq to Detect Genome-wide Double-Stranded Breaks in Plants.
Animal and plant cells have repair capabilities to combat DNA damage. DNA damage and repair dynamics can be determined by technologies such as IDLV capture, BLESS, HTGTS, digenome-seq, and GUIDE-seq. Here we highlight GUIDE-seq, a technology used in therapeutics, and envision its application in plants.
Provincial State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China; College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China; Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA.
Lina
L
Shi
Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA.
Xiaoqing
X
Tang
Provincial State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China; College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China; Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA. Electronic address: gtang1@mtu.edu.
Guiliang
G
Tang
D053903:DNA Breaks, Double-Stranded; D053904:DNA Breaks, Single-Stranded; D004249:DNA Damage; D005821:Genetic Techniques; D018745:Genome, Plant / Q000235:genetics; D009841:Oligonucleotides / Q000235:genetics; D010944:Plants / Q000235:genetics*; D012680:Sensitivity and Specificity
D009841:Oligonucleotides
BLESS; CRISPR/Cas9; DNA damage repair/response; GUIDE-seq; HTGTS; IDLV capture; PTO linkage; digenome-seq; double-stranded breaks; double-stranded oligonucleotide; single-stranded oligonucleotide