Effects of melatonin on cytokine release and healing of colonic anastomoses in an experimental sepsis model.
BACKGROUND
The present objective was to identify effects of early melatonin application on healing of anastomotic wound and inflammation in an experimental sepsis model.


METHODS
A total of 60 Wistar albino rats were divided into 2 groups. Cecal ligation puncture (CLP) and colonic resection anastomosis were performed on both the control group and the melatonin treatment group. Both groups were divided into 3 subgroups consisting of 10 rats each. One subgroup from each group underwent re-laparotomy at the 16th hour, the next on the 3rd day, and the final subgroup on the 7th day. Presently evaluated were effects of melatonin treatment of early sepsis on interleukin-6 (IL-6), interleukin-10 (IL-10), interferon gamma (INF-γ), and C-reactive protein (CRP) levels, as well as burst pressures (BPs), collagen and hydroxyproline (OHP) content of the anastomotic segments, histopathologic healing, immunohistochemical expressions, CD34, and transforming growth factor beta (TGF-ß).


RESULTS
IL-6 and INF-γ levels of the treatment group showed a significant decrease at the 16th hour and an increase on the 3rd and 7th postoperative days. IL-10 levels were significantly higher at the 16th hour and significantly lower on the 3rd and 7th postoperative days in the control group (p<0.001 for each). The treatment group also showed significantly higher capillary permeability, fibroblast proliferation, and collagen deposits (p<0.001 for each). CD34 expression was significantly increased in the treatment group on the 7th postoperative day (p=0.005).


CONCLUSIONS
Application of melatonin in early sepsis significantly improved colonic anastomotic healing in a rat model.
Department of General Surgery, Gaziosmanpasa University Faculty of Medicine, Tokat-Turkey.
Ömer Faik
ÖF
Ersoy
Department of General Surgery, Gaziosmanpasa University Faculty of Medicine, Tokat-Turkey.
Namık
N
Özkan
Department of General Surgery, Gaziosmanpasa University Faculty of Medicine, Tokat-Turkey.
Zeki
Z
Özsoy
Department of General Surgery, Gaziosmanpasa University Faculty of Medicine, Tokat-Turkey.
Hüseyin Ayhan
HA
Kayaoğlu
Department of General Surgery, Gaziosmanpasa University Faculty of Medicine, Tokat-Turkey. claritromisin@yahoo.com.
Erdinç
E
Yenidoğan
Department of General Surgery, Gaziosmanpasa University Faculty of Medicine, Tokat-Turkey.
Alper
A
Çelik
Department of Biochemistry, Gaziosmanpasa University Faculty of Medicine, Tokat-Turkey.
Aziz Fikret
AF
Özuğurlu
Department of Pathology, Atatürk Training and Research Hospital, Ankara-Turkey.
Ebru
E
Arabacı Çakır
Department of Histology and Embryology, Gazi University Faculty of Medicine, Ankara-Turkey.
Neşe
N
Lortlar
D000714:Anastomosis, Surgical; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D003106:Colon / Q000601:surgery; D007263:Infusions, Parenteral; D007371:Interferon-gamma / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007813:Laparotomy; D008297:Male; D008550:Melatonin / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000097:blood / Q000188:drug therapy* / Q000601:surgery; D014945:Wound Healing / Q000187:drug effects*
D000975:Antioxidants; D015850:Interleukin-6; D007371:Interferon-gamma; D008550:Melatonin