A Model for Transposition of the Colistin Resistance Gene mcr-1 by ISApl1.
Analysis of mcr-1-containing sequences identified a common ∼2,607-bp DNA segment that in many cases is flanked on one or both ends by ISApl1 We present evidence that mcr-1 is mobilized by an ISApl1 composite transposon which has, in some cases, subsequently lost one or both copies of ISApl1 We also show that mcr-1 can be mobilized in some circumstances by a single upstream copy of ISApl1 in conjunction with the remnants of a downstream ISApl1.
Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Erik
E
Snesrud
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Susu
S
He
Laboratoire de Microbiologie et Genetique Moleculaires, Centre National de la Recherche Scientifique, Toulouse, France.
Michael
M
Chandler
Department of Laboratory Medicine, Clinical Center, Microbiology Service, National Institutes of Health, Bethesda, Maryland, USA.
John P
JP
Dekker
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Alison B
AB
Hickman
Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Patrick
P
McGann
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA dyda@helix.nih.gov.
Fred
F
Dyda
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003091:Colistin / Q000494:pharmacology*; D004251:DNA Transposable Elements; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D016680:Genome, Bacterial; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics*
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; C000614037:MCR-1 protein, E coli; D003091:Colistin