Interaction Effects between Organochlorine Pesticides and Isoflavones In Vitro and In Vivo.
Organochlorine pesticides (OCPs) have caused increasing global concern due to their high toxicity, persistence, bioaccumulation, and significant adverse effects on human health. This study was to explore the interaction effects between OCPs and isoflavones. Six kinds of OCPs and 2 kinds of isoflavones-genistein and daidzein were included to study their effect on MCF-7 cells in vitro. Eighty-one female Sprague-Dawley rats were randomized to 9 groups according to factorial design to study the interaction effect between isoflavones and γ-HCH. Compared to organochlorine pesticides alone group, proliferation rate of MCF-7 cells was lower in 100 μmol/L genistein + organochlorine pesticides and 100 μmol/L daidzein + organochlorine pesticides group (p < 0.05). In vivo study showed that there are interaction effects on kidney weight and liver weight when treated with isoflavones and γ-HCH. The changes in uterine morphology and positive expression of ERα showed inhibition effects between isoflavones and γ-HCH. In conclusion, the data suggests that there are interactions between isoflavones and OCPs in vitro and in vivo.
Department of Environmental Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
Yunbo
0000-0001-6792-1451
Y
Zhang
Department of Environmental Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
Jipeng
0000-0001-6445-6427
J
Guo
Department of Environmental Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
Xiao
0000-0002-2492-6938
X
Zhang
Department of Environmental Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
Jingjing
0000-0002-3688-7825
J
Guo
Department of Environmental Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
Ming
0000-0002-5941-9485
M
Zhang
Department of Environmental Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
Yang
0000-0002-1946-816X
Y
Yang
Department of Environmental Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081, China.
Xiaolin
0000-0003-3891-7490
X
Na
D001681:Biological Assay / Q000379:methods*; D002849:Chromatography, Gas / Q000379:methods; D004785:Environmental Pollutants / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D007529:Isoflavones / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D008297:Male; D008875:Middle Aged; D010575:Pesticides / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013542:Sweat / Q000378:metabolism*
D004785:Environmental Pollutants; D006843:Hydrocarbons, Chlorinated; D007529:Isoflavones; D010575:Pesticides