Quantitative analysis of total serum glycome in human and mouse.
Model mice are frequently used in drug discovery research. Knowledge of similarities and differences between the mouse and human glycomes is critical when model mice are used for the discovery of glycan-related biomarkers and targets for therapeutic intervention. Since few comparative glycomic studies between human and mouse have been conducted, we performed a comprehensive comparison of the major classes of glycans in human and mouse sera using mass spectrometric and liquid chromatographic analyses. Up to 131 serum glycans, including N-glycans, free oligosaccharides (fOSs), glycosaminoglycans, O-glycans, and glycosphingolipid (GSL)-glycans, were quantified. In both serum samples, N-glycans were the most abundant in the total serum glycome, while fOSs were the least abundant. As expected, the diversity of sialic acid (i.e. Neu5Ac vs. Neu5Gc) was the major species difference between human and mouse in terms of N- and O-glycosylation, while GSL-glycomic profiles were completely different, even when the sialic acid diversity was taken into consideration. Furthermore, total serum glycomics of STAM mouse were unveiled as an initial step to identify novel biomarkers of liver diseases, with which we could identify several glycans with expression significantly increased or decreased expression.
Shionogi Innovation Center for Drug Discovery, Shionogi & Co., Ltd, Sapporo, Japan.
Yasunobu
Y
Yoshida
Laboratory of Medical and Functional Glycomics, Graduate School of Advanced Life Science, Hokkaido University, Sapporo, Japan.|Department of Orthopaedic Orthopaedic Surgery, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
Jun-Ichi
JI
Furukawa
Shionogi Innovation Center for Drug Discovery, Shionogi & Co., Ltd, Sapporo, Japan.
Shoichi
S
Naito
Shionogi Innovation Center for Drug Discovery, Shionogi & Co., Ltd, Sapporo, Japan.
Kenichi
K
Higashino
Shionogi Innovation Center for Drug Discovery, Shionogi & Co., Ltd, Sapporo, Japan.
Yoshito
Y
Numata
Laboratory of Medical and Functional Glycomics, Graduate School of Advanced Life Science, Hokkaido University, Sapporo, Japan. yshinohara@sci.hokudai.ac.jp.|Department of Pharmacy, Kinjo Gakuin University, Nagoya, Japan. yshinohara@sci.hokudai.ac.jp.
Yasuro
Y
Shinohara
D000818:Animals; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000096:biosynthesis* / Q000235:genetics; D002853:Chromatography, Liquid; D054794:Glycomics / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D051379:Mice; D020543:Proteome / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome
Glycoproteomics; Glycosylation; HPLC; MALDI-TOF-MS; Serum glycomics; Species difference