Prospective study of the clinical performance of three BACTEC media in a modern emergency department: Plus Aerobic/F, Plus Anaerobic/F, and Anaerobic Lytic/F.
The performance of 3 blood culture bottles (BACTEC Plus Aerobic/F, Plus Anaerobic/F, and Anaerobic Lytic/F) were analyzed with clinical specimens collected from 688 Emergency Department patients. A total of 270 strains belonging to 33 species were identified, with E. coli and S. aureus as the most frequently detected. Overall recovery rate (RR) of bacteria and yeast was equivalent in the Plus Aerobic/F vials (208 of 270 isolates; 77.0%) and Anaerobic Lytic/F vials (206 isolates; 76.3%) and significantly better than in the Plus Anaerobic/F vials (189 isolates; 70.0%). Median time to detection (TTD) was earliest with the Anaerobic Lytic/F vials (12.0h) compared with the Plus Aerobic/F (14.6h) and Plus Anaerobic/F vials (15.4h). Positivity rate (PR) was similar for Anaerobic Lytic/F vials (76.9%) and Plus Aerobic/F vials (76.5%), but better if compared with Plus Anaerobic/F vials (69.4%). The PR and TTD for the combination of Plus Aerobic/F with Anaerobic Lytic/F (94.5% and 12.3h, respectively) was significantly better than with Plus Aerobic/F with Plus Anaerobic/F (87.8% and 14.1h).
Laboratory of Microbiology, Hospital "Santi Antonio e Biagio e C. Arrigo", Street Venezia, 16-15121 Alessandria, Italy. Electronic address: arocchetti@ospedale.al.it.
Andrea
A
Rocchetti
Laboratory of Microbiology, Hospital "Santi Antonio e Biagio e C. Arrigo", Street Venezia, 16-15121 Alessandria, Italy.
Luigi
L
Di Matteo
Department of Medical Sciences, University of Torino, C.So Dogliotti, 14-10126 Torino, Italy.
Paolo
P
Bottino
Department of Sciences & Technologie de la santé, Université Joseph Fourier/Université Pierre Mendès, Avenue Marie Reynoard, 14-38100 Grenoble, France.
Benjamin
B
Foret
University of Eastern Piedmont, Department of Science and Technology Innovation, Viale Teresa Michel, 1-15121 Alessandria, Italy.
Elisa
E
Gamalero
Emergency Department, Hospital "Santi Antonio e Biagio e C. Arrigo", Street Venezia, 16-15121 Alessandria, Italy.
Alessandra
A
Calabresi
Emergency Department, Hospital "Santi Antonio e Biagio e C. Arrigo", Street Venezia, 16-15121 Alessandria, Italy.
Gianluca
G
Guido
Emergency Department, Hospital "Santi Antonio e Biagio e C. Arrigo", Street Venezia, 16-15121 Alessandria, Italy.
Ivo
I
Casagranda
D000332:Aerobiosis; D000368:Aged; D000693:Anaerobiosis; D016470:Bacteremia / Q000382:microbiology; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D001424:Bacterial Infections / Q000382:microbiology; D001769:Blood / Q000382:microbiology*; D000071997:Blood Culture / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D004636:Emergency Service, Hospital; D004926:Escherichia coli / Q000302:isolation & purification / Q000472:pathogenicity; D005260:Female; D016469:Fungemia / Q000382:microbiology; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008297:Male; D009181:Mycoses / Q000382:microbiology; D011446:Prospective Studies; D018805:Sepsis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000472:pathogenicity; D013997:Time Factors; D015003:Yeasts / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity
D003470:Culture Media
BACTEC FX; Blood culture; ED; Lytic; Sepsis