On chip purification of hiPSC-derived cardiomyocytes using a fishnet-like microstructure.
Human induced pluripotent stem cells (hiPSCs) can be differentiated at high efficiency into cells of a targeting type but the resulting cell population has to be of high purity for clinical therapies to avoid teratomas. Herein, we report a microfluidic device with integrated and surface functionalised fishnet-like structures for specific cell capture. With the help of a flow derivation surface pattern, cells in solution are forced to cross the fishnet-like structure, resulting in high efficiency and selective retention of a chosen cell population. A suspension of hiPSCs and hiPSC-derived cardiomyocytes were used for device function validation. We found that a hiPSC capture rate over 80% can be achieved along with a remarkable increase in the CM population rate in the recovered suspension without affecting cell viability.
Institute for Integrated Cell-Material Science (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan. Department of Physics, Engineering Research Center for Nanophotonics & Advanced Instrument, Ministry of Education, East China Normal University, Shanghai 200062, People's Republic of China.
Xin
X
Li
Leqian
L
Yu
Junjun
J
Li
Itsunari
I
Minami
Minako
M
Nakajima
Yuichiro
Y
Noda
Hidetoshi
H
Kotera
Li
L
Liu
Yong
Y
Chen
D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D002469:Cell Separation; D002470:Cell Survival; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D056656:Lab-On-A-Chip Devices; D008856:Microscopy, Fluorescence; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D013499:Surface Properties