A combination of He-Ne laser irradiation and exogenous NO application efficiently protect wheat seedling from oxidative stress caused by elevated UV-B stress.
The elevated ultraviolet-B (UV-B) stress induces the accumulation of a variety of intracellular reactive oxygen species (ROS), which seems to cause oxidative stress for plants. To date, very little work has been done to evaluate the biological effects of a combined treatment with He-Ne laser irradiation and exogenous nitric oxide (NO) application on oxidative stress resulting from UV-B radiation. Thus, our study investigated the effects of a combination with He-Ne laser irradiation and exogenous NO treatment on oxidative damages in wheat seedlings under elevated UV-B stress. Our data showed that the reductions in ROS levels, membrane damage parameters, while the increments in antioxidant contents and antioxidant enzyme activity caused by a combination with He-Ne laser and exogenous NO treatment were greater than those of each individual treatment. Furthermore, these treatments had a similar effect on transcriptional activities of plant antioxidant enzymes. This implied that the protective effects of a combination with He-Ne laser irradiation and exogenous NO treatment on oxidative stress resulting from UV-B radiation was more efficient than each individual treatment with He-Ne laser or NO molecule. Our findings might provide beneficial theoretical references for identifying some effective new pathways for plant UV-B protection.
Analysis and Testing Center, Shanxi Normal University, Linfen, 041004, China.|Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, 041004, China.
Yongfeng
Y
Li
Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, 041004, China. limeigao1122@126.com.|College of Life Science, Shanxi Normal University, Linfen, 041004, China. limeigao1122@126.com.
Limei
L
Gao
Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, 041004, China. hhwrsl@163.com.|College of Life Science, Shanxi Normal University, Linfen, 041004, China. hhwrsl@163.com.
Rong
R
Han
D000975:Antioxidants / Q000378:metabolism; D054020:Lasers, Gas; D009569:Nitric Oxide / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D014908:Triticum / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D009569:Nitric Oxide
Combination; He-Ne laser; Nitric oxide; Oxidative stress; UV-B stress