Fabrication of hydrogel based nanocomposite scaffold containing bioactive glass nanoparticles for myocardial tissue engineering.
Selecting suitable cell sources and angiogenesis induction are two important issues in myocardial tissue engineering. Human endometrial stromal cells (EnSCs) have been introduced as an abundant and easily available resource in regenerative medicine. Bioactive glass is an agent that induces angiogenesis and has been studied in some experiments. The aim of this study was to investigate in vitro differentiation capacity of endometrial stem cells into cardiomyocyte lineage and to evaluate capability of bioactive glass nanoparticles toward EnSCs differentiation into endothelial lineage and angiogenesis on hydrogel scaffold. Our findings suggests that endometrial stem cells could be programmed into cardiomyocyte linage and considered a suitable cell source for myocardial regeneration. This experiment also revealed that inclusion of bioactive glass nanoparticles in hydrogel scaffold could improve angiogenesis through differentiating EnSCs toward endothelial lineage and increasing level of vascular endothelial growth factor secretion.
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Zahra
Z
Barabadi
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Mahmoud
M
Azami
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Esmaeel
E
Sharifi
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Roya
R
Karimi
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Nasrin
N
Lotfibakhshaiesh
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Reza
R
Roozafzoon
Anatomy Department, Iran University of Medical Sciences, Tehran, Iran.
Mohammad Taghi
MT
Joghataei
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Brain and Spinal Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: jafar_ai@tums.ac.ir.
Jafar
J
Ai
D002152:Calorimetry, Differential Scanning; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology; D005260:Female; D005898:Glass / Q000737:chemistry*; D006321:Heart / Q000502:physiology; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000494:pharmacology; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D053761:Nanocomposites / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D016062:Porosity; D012038:Regeneration / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D013818:Thermogravimetry; D023822:Tissue Engineering; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
D042461:Vascular Endothelial Growth Factor A; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
Bioactive glass; Hydrogel; Myocardial tissue engineering; Nanocomposite scaffold