Levosimendan, a new therapeutic approach to prevent delayed cerebral vasospasm after subarachnoid hemorrhage?
Under physiological cerebral conditions, levosimendan, a calcium-channel sensitizer, has a dose-dependent antagonistic effect on prostaglandin F2alpha (PGF)-induced vasoconstriction. This circumstance could be used in antagonizing delayed cerebral vasospasm (dCVS), one of the main complications after subarachnoid hemorrhage (SAH), leading to delayed cerebral ischemia and ischemic neurological deficits. Data already exist that identified neuroprotective effects of levosimendan in a traumatic brain injury model and additionally, it has been proven that this compound prevents narrowing of the basilar artery (BA) luminal area after SAH in an in vitro rabbit model. Takotsubo cardiomyopathy, a severe ventricular dysfunction, is also a well-known complication after SAH, associated with pulmonary edema and prolonged intubation.



The polypeptide endothelin-1 (ET-1) plays a key role in the development of dCVS after SAH. Therefore, the aim of the present investigation was to detect functional interactions between the calcium-sensitizing and the ET-1-dependent vasoconstriction after experimental-induced SAH; interactions between levosimendan and a substrate-specific vasorelaxation in the BA were also examined. It was reviewed whether levosimendan has a beneficial influence on endothelin(A) and/or endothelin(B1) receptors (ET-(A) and ET-(B1) receptors) in cerebral vessels after SAH. We also examined whether this drug could have antagonistic effects on a PGF-induced vasoconstriction.



Under treatment with levosimendan after SAH, the endothelin system seems to be affected. The ET-1-induced contraction is decreased, not significantly. In addition, we detected changes in the nitric oxide-cyclic guanosine monophosphate (NO-cGMP) pathway. Preincubation with levosimendan causes a modulatory effect on the ET-(B1) receptor-dependent vasorelaxation. It induces an upregulation of the NO-cGMP pathway with a significantly increased relaxation. Even after PGF-induced precontraction a dose-dependent relaxation was registered, which was significantly higher (Emax) and earlier (pD2) compared to the concentration-effect curve without levosimendan.



After experimental-induced dCVS, levosimendan seems to restore the well-known impaired function of the vasorelaxant ET-(B1) receptor. Levosimendan also reversed the PGF-induced contraction dose-dependently. Both of these mechanisms could be used for antagonizing dCVS in patients suffering SAH. Levosimendan could even be used additionally in treating patients developing takotsubo cardiomyopathy.
Department of Neurosurgery, Johann Wolfgang Goethe-University, Frankfurt am Main, Germany.
Juergen
J
Konczalla
Department of Neurosurgery, Inselspital University Hospital Bern, Freiburgstrasse 4, 3010, Bern, Switzerland. stefan.wanderer@insel.ch.
Stefan
S
Wanderer
Department of Neurosurgery, Johann Wolfgang Goethe-University, Frankfurt am Main, Germany.
Jan
J
Mrosek
Department of Neurosurgery, Rheinische Friedrich-Wilhelms-University, Bonn, Germany.
Erdem
E
Gueresir
Department of Neurosurgery, Rheinische Friedrich-Wilhelms-University, Bonn, Germany.
Patrick
P
Schuss
Department of Neurosurgery, Johann Wolfgang Goethe-University, Frankfurt am Main, Germany.
Johannes
J
Platz
Department of Neurosurgery, Johann Wolfgang Goethe-University, Frankfurt am Main, Germany.
Volker
V
Seifert
Department of Neurosurgery, Rheinische Friedrich-Wilhelms-University, Bonn, Germany.
Hartmut
H
Vatter
D000818:Animals; D006835:Hydrazones / Q000008:administration & dosage / Q000627:therapeutic use*; D011724:Pyridazines / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D000077464:Simendan; D013345:Subarachnoid Hemorrhage / Q000150:complications / Q000188:drug therapy*; D020301:Vasospasm, Intracranial / Q000188:drug therapy* / Q000209:etiology / Q000517:prevention & control
D006835:Hydrazones; D011724:Pyridazines; D000077464:Simendan
Delayed cerebral vasospasm; ET-(A) receptor; ET-(B1) receptor; ET-1; Endothelin; Levosimendan; PGF; Prostaglandin F2alpha; SAH; Subarachnoid hemorrhage; Takotsubo cardiomyopathy; dCVS