Changes in energetic metabolism of Biomphalaria glabrata (Mollusca, Planorbidae) in response to exogenous calcium.
Calcium is considered an essential element for the metabolism of aquatic snail Biomphalaria glabrata (Say, 1818), intermediate host of Schistosoma mansoni Sambon, 1907 in Brazil, and represents a limiting factor to its distribution and adaptation to the environment. This study investigated the effect of different concentrations of exogenous CaCO3 on the energetic metabolism of B. glabrata for better understanding the physiological interference of chemical elements dissolved in the environment with the physiology of this species. Sixty-day-old snails were distributed into six groups, five exposed to different concentrations of CaCO3 (20, 40, 60, 80 and 100 mg/L) and a control group. The exposure to CaCO3 was assessed over time, with analysis of 15 snails of each group in the following intervals: 1, 14, 21 or 30 days for hemolymph extraction. Concentrations of calcium and glucose in the hemolymph were determined by commercial kits, and organic acids were extracted using an ion exchange column and analyzed by high-performance liquid chromatography. Concentration of calcium in the hemolymph showed no significant difference (p>0.05) from the control group and between the concentrations tested. Concentration of glucose decreased (p<0.05) in the treatments of exposure to 20 and 40 mg/L and increased when exposed to 80 and 100 mg/L CaCO3 compared to control and to other concentrations tested over 30 days. The organic acids pyruvate, oxaloacetate, citrate, succinate, fumarate, beta-hydroxybutyrate and lactate presented increased concentrations, while propionate and acetoacetate, decreased concentrations, when exposed to CaCO3 compared to control. Considering the influence of different periods of exposure to CaCO3, on the 14th day, there were stronger alterations in the metabolism of B. glabrata. In conclusion, exposure to CaCO3 reduced the concentration of glucose, which is metabolized into pyruvate, the final product of glycolysis, and also influenced the energetic metabolism pathways, indicating an aerobic or partially anaerobic functioning.
Ciências Exatas e Tecnológicas - CCET, Universidade Estadual de Goiás - UEG, Câmpus Anápolis, BR 153, 3105, CEP 75132-903, Anápolis, GO, Brazil.|Unidade Universitária de Ciências Exatas e Tecnológicas - UnUCET, Universidade Estadual de Goiás - UEG, BR 153, 3105 CEP 75132-903, Anápolis, GO, Brazil.
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LD
Silva
Ciências Exatas e Tecnológicas - CCET, Universidade Estadual de Goiás - UEG, Câmpus Anápolis, BR 153, 3105, CEP 75132-903, Anápolis, GO, Brazil.
V C S
VCS
Amaral
Laboratório de Estudos da Relação Parasito-Hospedeiro, Instituto de Patologia Tropical e Saúde Pública - IPTSP, Universidade Federal de Goiás - UFG, Rua 235, s/n, Setor Universitário, CEP 74650-050, Goiânia, GO, Brazil.
M C
MC
Vinaud
Laboratório de Estudos da Relação Parasito-Hospedeiro, Instituto de Patologia Tropical e Saúde Pública - IPTSP, Universidade Federal de Goiás - UFG, Rua 235, s/n, Setor Universitário, CEP 74650-050, Goiânia, GO, Brazil.
A M
AM
Castro
Laboratório de Estudos da Relação Parasito-Hospedeiro, Instituto de Patologia Tropical e Saúde Pública - IPTSP, Universidade Federal de Goiás - UFG, Rua 235, s/n, Setor Universitário, CEP 74650-050, Goiânia, GO, Brazil.
H H A
HHA
Rezende
Laboratório de Estudos da Relação Parasito-Hospedeiro, Instituto de Patologia Tropical e Saúde Pública - IPTSP, Universidade Federal de Goiás - UFG, Rua 235, s/n, Setor Universitário, CEP 74650-050, Goiânia, GO, Brazil.
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DB
Santos
Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz - FIOCRUZ, Avenida Brasil, 4365, Manguinhos, CEP 21040-360, Rio de Janeiro, RJ, Brazil.
C C
CC
Mello-Silva
Laboratório de Estudos da Relação Parasito-Hospedeiro, Instituto de Patologia Tropical e Saúde Pública - IPTSP, Universidade Federal de Goiás - UFG, Rua 235, s/n, Setor Universitário, CEP 74650-050, Goiânia, GO, Brazil.
J C B
JCB
Bezerra
D000818:Animals; D001700:Biomphalaria / Q000187:drug effects / Q000378:metabolism*; D002119:Calcium Carbonate / Q000008:administration & dosage / Q000378:metabolism*; D002136:Calcium, Dietary / Q000008:administration & dosage / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism; D011897:Random Allocation
D002136:Calcium, Dietary; D002119:Calcium Carbonate