Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusFlammulina velutipes.
The initiation of sexual development in the important edible and medicinal mushroomFlammulina velutipesis controlled by special genes at two different, independent, mating type (MAT) loci: HD and PR. We expanded our understanding of theF. velutipesmating type system by analyzing the MAT loci from a series of strains. The HD locus ofF. velutipeshouseshomeodomaingenes (Hdgenes) on two separated locations: sublocus HD-a and HD-b. The HD-b subloci contained strain-specificHd1/Hd2gene pairs, and crosses between strains with different HD-b subloci indicated a role in mating. The function of the HD-a sublocus remained undecided. Many, but not all strains contained the same conservedHd2gene at the HD-a sublocus. The HD locus usually segregated as a whole, though we did detect one new HD locus with a HD-a sublocus from one parental strain, and a HD-b sublocus from the other. The PR locus ofF. velutipescontainedpheromone receptor(STE3) andpheromone precursor(Pp) genes at two locations, sublocus PR-a and PR-b. PR-a and PR-b both contained sets of strain-specificSTE3andPpgenes, indicating a role in mating. PR-a and PR-b cosegregated in our experiments. However, the identification of additional strains with identical PR-a, yet different PR-b subloci, demonstrated that PR subloci can recombine within the PR locus. In conclusion, at least three of the four MAT subloci seem to participate in mating, and new HD and PR loci can be generated through intralocus recombination inF. velutipes.
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|Department of Fungal Resource, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China.
Wei
W
Wang
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Lingdan
L
Lian
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Ping
P
Xu
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Tiansheng
T
Chou
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Irum
I
Mukhtar
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Aron
A
Osakina
College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Muhammad
M
Waqas
College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Bingzhi
B
Chen
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Xinrui
X
Liu
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Fang
F
Liu
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China mrcfafu@163.com arendvanpeer@gmail.com.
Baogui
B
Xie
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China mrcfafu@163.com arendvanpeer@gmail.com.
Arend F
AF
van Peer
Flammulina velutipes; Genetics of Sex; homeodomain; mating type; pheromone receptor