Inhibiting HMGB1 Reduces Cerebral Ischemia Reperfusion Injury in Diabetic Mice.
High mobility group box1 (HMGB1) promotes inflammatory injury, and accumulating evidence suggests that it plays a key role in brain ischemia reperfusion (I/R), as well as the development of diabetes mellitus (DM). The purpose of this study was to investigate whether HMGB1 plays a role in brain I/R in a DM mouse model. Diabetes mellitus was induced by a high-calorie diet and streptozotocin treatment, and cerebral ischemia was induced by middle cerebral artery occlusion. We examined HMGB1 levels following cerebral I/R injury in DM and non-DM mice and evaluated the influence of altered HMGB1 levels on the severity of cerebral injury. Serum HMGB1 levels and the inflammatory factors IL-1β, IL-6, and inflammation-related enzyme iNOS were significantly elevated in DM mice with brain I/R compared with non-DM mice with brain I/R. Blocking HMGB1 function by intraperitoneal injection of anti-HMGB1 neutralizing antibodies reversed the inflammatory response and the extent of brain damage, suggesting that HMGB1 plays an important role in cerebral ischemic stroke in diabetic mice.
The Military General Hospital of Beijing, PLA, Beijing, 100700, People's Republic of China.|Jining First People Hospital, Jining, 272011, People's Republic of China.
Chong
C
Wang
Jining First People Hospital, Jining, 272011, People's Republic of China.
Jie
J
Jiang
Jinan Central Hospital, Jinan, 250012, People's Republic of China.
Xiuping
X
Zhang
Jinan Central Hospital, Jinan, 250012, People's Republic of China.
Linjie
L
Song
Graduate School, Weifang Medical University, Weifang, 261053, People's Republic of China.
Kai
K
Sun
The Military General Hospital of Beijing, PLA, Beijing, 100700, People's Republic of China. ruxiang_xu@163.com.|Affiliated Bayi Brain Hospital, General Hospital of Beijing, Military Region, No. 5, Nanmencang, Dongcheng District, Beijing, 100000, People's Republic of China. ruxiang_xu@163.com.
Ruxiang
R
Xu
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000627:therapeutic use; D002545:Brain Ischemia / Q000188:drug therapy* / Q000517:prevention & control; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D024243:HMGB1 Protein / Q000037:antagonists & inhibitors* / Q000097:blood / Q000276:immunology; D020244:Infarction, Middle Cerebral Artery; D007249:Inflammation; D051379:Mice; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000517:prevention & control
D000906:Antibodies; D024243:HMGB1 Protein
HMGB1; brain ischemia reperfusion; diabetes mellitus