Termitarium-inhabiting Bacillus endophyticus TSH42 and Bacillus cereus TSH77 colonizing Curcuma longa L.: isolation, characterization, and evaluation of their biocontrol and plant-growth-promoting activities.
Bacillus strains were isolated from termitarium soil and screened for their antifungal activity through the production of diffusible and volatile metabolites. Further, the bacterial strains that showed antifungal activity were evaluated for their biocontrol potential on the basis of their plant-growth-promoting attributes. Termitarium-inhabiting Bacillus strains TSH42 and TSH77 significantly reduced the growth of pathogenic fungus Fusarium solani, controlled the symptoms of rhizome rot in turmeric (Curcuma longa L.), and demonstrated various plant-growth-promoting traits in different in vitro assays. On the basis of morphological, physiological, biochemical, and 16S rDNA characteristics, isolates TSH42 and TSH77 were identified as Bacillus endophyticus (KT379993) and Bacillus cereus (KT379994), respectively. Through liquid chromatography - mass spectrometry analysis, acidified cell-free culture filtrate (CFCF) of B. cereus TSH77 was shown to contain surfactin and fengycin, while CFCF of B. endophyticus TSH42 contained iturin in addition to surfactin and fengycin. Treatment of the turmeric (C. longa L.) plants with TSH42 and TSH77 significantly reduced the percentage incidence of rhizome rot disease caused by F. solani. The same treatment also increased the fresh rhizome biomass and plant growth in greenhouse conditions.
a Department of Botany and Microbiology, Gurukul Kangri University, Haridwar 249-404, Uttrakhand, India.
Ankit Kumar
AK
Chauhan
a Department of Botany and Microbiology, Gurukul Kangri University, Haridwar 249-404, Uttrakhand, India.
Dinesh Kumar
DK
Maheshwari
b Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, 79 Gobong-ro, Iksan-si-570-752, Joellabuk-do (Jeonbuk), Republic of Korea.
Kangmin
K
Kim
c Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
Vivek K
VK
Bajpai
D000898:Antibiosis; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D001407:Bacillus / Q000302:isolation & purification / Q000502:physiology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry; D030024:Curcuma / Q000254:growth & development / Q000382:microbiology*; D005670:Fusarium / Q000187:drug effects / Q000502:physiology; D050296:Microbial Viability; D010935:Plant Diseases / Q000382:microbiology; D027343:Rhizome / Q000254:growth & development / Q000382:microbiology*; D017262:Siderophores / Q000096:biosynthesis; D012988:Soil Microbiology
D000935:Antifungal Agents; D001426:Bacterial Proteins; D017262:Siderophores
Bacillus; Fusarium solani; PGPR; antifongique; antifungal; bacilles; curcuma; termitarium; termitière; turmeric; volatil; volatile