Plasma and urine DNA levels are related to microscopic hematuria in patients with bladder urothelial carcinoma.
a) Objective: An increase in cell-free DNA was observed in the plasma of many cancer patients. This major biomarker can be used to differentiate patients with malignant neoplasms from those with benign neoplasms or healthy patients. Depending on the characteristic of the tumor, there are qualitative variations in the circulating cell-free DNA. Today, studies on the concentration of fragments of circulating cell-free DNA and their respective sizes in patients with bladder cancer are not plentiful in the literature. A 100% effective plasma tumor marker, which would help in the diagnosis and follow-up of bladder cancer, is yet to be developed; therefore, cell-free DNA levels in the plasma may represent a valuable biomarker for the diagnosis, prognosis and follow-up of patients with this type of tumor. b) Design and methods: In this study we analyze the kinetics of plasma and urine DNA concentrations in patients with bladder cancer, relating them to the other clinical laboratory variables. c) Results: Patients with hematuria showed a positive correlation with urine DNA. d) Conclusion: An increase in plasma and urine DNA was unprecedentedly reported over time, a fact that may come in handy in the prognosis of patients. Furthermore, microscopic haematuria is correlated with plasma and urinary DNA levels.
Disciplina de Urologia, Faculade de Medicina do ABC, Santo André, SP, Brazil.
Eduardo Ferreira Pedroso
EF
de Almeida
Disciplina de Urologia, Faculade de Medicina do ABC, Santo André, SP, Brazil.
Tomás Elias
TE
Abdalla
Disciplina de Urologia, Faculade de Medicina do ABC, Santo André, SP, Brazil.
Tiago Pedromonico
TP
Arrym
Laboratório de Análises Clínicas da Faculdade de Medicina do ABC, Santo André, SP, Brazil.
Pamela
P
de Oliveira Delgado
Disciplina de Urologia, Faculade de Medicina do ABC, Santo André, SP, Brazil.
Marcelo Langer
ML
Wroclawski
Laboratório de Análises Clínicas da Faculdade de Medicina do ABC, Santo André, SP, Brazil.
Beatriz
B
da Costa Aguiar Alves
Laboratório de Análises Clínicas da Faculdade de Medicina do ABC, Santo André, SP, Brazil.
Flávia
F
de S Gehrke
Universidade Federal de São Paulo, Diadema, SP, Brazil.
Ligia Ajaime
LA
Azzalis
Universidade Federal de São Paulo, Diadema, SP, Brazil.
Sarah
S
Alves
Disciplina de Urologia, Faculade de Medicina do ABC, Santo André, SP, Brazil.
Marcos
M
Tobias-Machado
Disciplina de Urologia, Faculade de Medicina do ABC, Santo André, SP, Brazil.
Antonio Carlos
AC
de Lima Pompeo
Laboratório de Análises Clínicas da Faculdade de Medicina do ABC, Santo André, SP, Brazil; Universidade Federal de São Paulo, Diadema, SP, Brazil. Electronic address: profferfonseca@gmail.com.
Fernando Luiz Affonso
FL
Fonseca
D000368:Aged; D004247:DNA / Q000097:blood / Q000378:metabolism* / Q000652:urine; D005260:Female; D006417:Hematuria / Q000097:blood / Q000378:metabolism* / Q000652:urine; D006801:Humans; D008297:Male; D001749:Urinary Bladder Neoplasms / Q000097:blood / Q000378:metabolism* / Q000652:urine
D004247:DNA
Bladder cancer; Hematuria; Plasma DNA; Prognosis; Urine DNA