Antimicrobial binding and growth kinetics in BacT/ALERT® FA Plus and BACTEC® Aerobic/F Plus blood culture media.
The capacity of absorbent beads in BacT/ALERT® FA Plus and BACTEC® Aerobic/F Plus blood culture bottles to bind and neutralize antibiotics was compared. Binding was established using reverse-phase HPLC, and inactivation was based on the recovery of susceptible test stains from simulated blood cultures. The FA Plus medium demonstrated more rapid and better overall binding kinetics for each drug tested, resulting in significantly better overall recovery rates. Differences in time to detection favored the FA Plus medium for three drug/organism combinations and Aerobic/F Plus for two.
bioMérieux, Inc., 100 Rodolphe Street, Durham, NC, 27712, USA.
D
D
Lovern
bioMérieux, Inc., 100 Rodolphe Street, Durham, NC, 27712, USA.
B
B
Katzin
bioMérieux, Inc., 100 Rodolphe Street, Durham, NC, 27712, USA.
K
K
Johnson
bioMérieux, Inc., 100 Rodolphe Street, Durham, NC, 27712, USA.
D
D
Broadwell
bioMérieux, Inc., 100 Rodolphe Street, Durham, NC, 27712, USA.
E
E
Miller
bioMérieux, Inc., 100 Rodolphe Street, Durham, NC, 27712, USA.
A
A
Gates
bioMérieux, Inc., 100 Rodolphe Street, Durham, NC, 27712, USA.
P
P
Deol
bioMérieux, Inc., 100 Rodolphe Street, Durham, NC, 27712, USA.
K
K
Doing
bioMérieux, SA, La Balme Les Grottes, France.
A
A
van Belkum
bioMérieux, Inc., St. Louis, MO, USA.
C
C
Marshall
bioMérieux, Inc., St. Louis, MO, USA.
E
E
Mathias
bioMérieux, Inc., 100 Rodolphe Street, Durham, NC, 27712, USA. william.dunne@biomerieux.com.
W M
WM
Dunne
D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification*; D000071997:Blood Culture / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D003470:Culture Media