Identification of Gastric Cancer Biomarkers Using 1H Nuclear Magnetic Resonance Spectrometry.
Existing gastric cancer diagnosing methods were invasive, hence, a reliable non-invasive gastric cancer diagnosing method is needed. As a starting point, we used 1H NMR for identifying gastric cancer biomarkers using a panel of gastric cancer spheroids and normal gastric spheroids. We were able to identify 8 chemical shift biomarkers for gastric cancer spheroids. Our data suggests that the cancerous and non-cancerous spheroids significantly differ in the lipid composition and energy metabolism. These results encourage the translation of these biomarkers into in-vivo gastric cancer detection methodology using MRI-MS.
Department of Biomedical Engineering, National University of Singapore, Singapore, Singapore.
Gokula Krishnan
http://orcid.org/0000-0001-5365-4348
GK
Ramachandran
Department of Haematology-Oncology, National University Cancer Institute, Singapore (NCIS), Singapore.
Wei Peng
WP
Yong
Department of Biomedical Engineering, National University of Singapore, Singapore, Singapore.
Chen Hua
CH
Yeow
D014408:Biomarkers, Tumor / Q000032:analysis*; D045744:Cell Line, Tumor; D005240:Feasibility Studies; D006031:Glycosylation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010743:Phospholipids / Q000378:metabolism; D018874:Spheroids, Cellular; D013274:Stomach Neoplasms / Q000175:diagnosis*
D014408:Biomarkers, Tumor; D010743:Phospholipids