Effect of fructose on promoting fengycin biosynthesis in Bacillus amyloliquefaciens fmb-60.
OBJECTIVE
To explore the influence of carbohydrates on the production of fengycin by Bacillus amyloliquefaciens fmb-60.


RESULTS
Fermentation and gene expression levels were examined following culture supplementation with fructose, arabinose and sorbitol. Compared with other carbohydrates, fructose most efficiently increased the concentration of the essential component amino acids for fengycin synthesis in the cell. The maximum production of fengycin was found to be 392·87 mg l-1, increased by 76% in fed-batch fermentation. Furthermore, fructose enhanced the biosynthesis of fengycin by increasing the expression of fengycin synthetase genes (fenA, fenB, fenC, fenD, fenE and fenTE) and regulatory genes (comA, degU, degQ and sigma H).


CONCLUSIONS
Fructose is important for fengycin production in B. amyloliquefaciens fmb-60, and it could enhance fengycin biosynthesis by raising the expression of the fengycin synthetase genes and upregulating signal factors.


CONCLUSIONS
The results identify regulatory mechanisms involved in fengycin biosynthesis and provide an effective method to enhance production of fengycin.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
H
H
Lu
Department of Bioengineering and Food, Bengbu College, Bengbu, Anhui, China.
S
S
Qian
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
U
U
Muhammad
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
X
X
Jiang
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
J
J
Han
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Z
Z
Lu
D000069977:Bacillus amyloliquefaciens / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005632:Fructose / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D055666:Lipopeptides / Q000096:biosynthesis*; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism
D055666:Lipopeptides; C049972:fengycin; D005632:Fructose; D010453:Peptide Synthases; C110972:fengycin synthetase

Bacillus amyloliquefaciens
; Fructose; fed-batch; fengycin; gene expression