Simultaneous mutation of G275A and P276A in the matrix protein of Newcastle disease virus decreases virus replication and budding.
The matrix (M) protein of Newcastle disease virus (NDV) is a highly conserved hydrophobic viral protein. In some paramyxoviruses (measles virus and Sendai virus), the paired glycine (G) near the C terminus of the M protein may form a turn that mediates the specific interaction with the cell membrane. Similar amino acids (glycine-proline [GP], at position 275-276) exist in the M protein of NDV. However, the role of these residues in the replication and pathogenicity of NDV is unknown. In this study, recombinant NDV with the sequence GP/AA or LGP/GGL in the M protein was generated to investigate the role of this conserved sequence. Budding experiments on the mutant viruses revealed that the GP/AA mutation reduced virus budding and virus replication in DF-1 cells; biological characterization revealed attenuated virulence and pathogenicity in chickens, indicating that the GP sequence plays a critical role in the life cycle of the virus.
College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
Haixu
H
Xu
College of Animal Science, Guizhou University, Guiyang, 550025, China.
Zhiqiang
Z
Duan
College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
Yu
Y
Chen
College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
Jiajia
J
Liu
College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
Xin
X
Cheng
College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
Jingjing
J
Liu
College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
Jie
J
Zhu
College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.|Ministry of Educational Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, 225009, China.
Xiaoquan
X
Wang
College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.|Ministry of Educational Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, 225009, China.
Xiaowen
X
Liu
College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.|Ministry of Educational Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, 225009, China.
Shunlin
S
Hu
College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China. xfliu@yzu.edu.cn.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China. xfliu@yzu.edu.cn.|Ministry of Educational Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, 225009, China. xfliu@yzu.edu.cn.
Xiufan
X
Liu
D000818:Animals; D002460:Cell Line; D002645:Chickens; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D020125:Mutation, Missense; D009522:Newcastle disease virus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D059386:Reverse Genetics; D019562:Viral Load; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D014774:Virulence; D057074:Virus Release; D014779:Virus Replication
D050505:Mutant Proteins; D014763:Viral Matrix Proteins