A Panel of Embryonic Stem Cell Lines Reveals the Variety and Dynamic of Pluripotent States in Rabbits.
Conventional rabbit embryonic stem cell (ESC) lines are derived from the inner cell mass (ICM) of pre-implantation embryos using methods and culture conditions that are established for primate ESCs. In this study, we explored the capacity of the rabbit ICM to give rise to ESC lines using conditions similar to those utilized to generate naive ESCs in mice. On single-cell dissociation and culture in fibroblast growth factor 2 (FGF2)-free, serum-supplemented medium, rabbit ICMs gave rise to ESC lines lacking the DNA-damage checkpoint in the G1 phase like mouse ESCs, and with a pluripotency gene expression profile closer to the rabbit ICM/epiblast profiles. These cell lines can be converted to FGF2-dependent ESCs after culture in conventional conditions. They can also colonize the rabbit pre-implantation embryo. These results indicate that rabbit epiblast cells can be coaxed toward different types of pluripotent stem cells and reveal the dynamics of pluripotent states in rabbit ESCs.
Univ Lyon, Université Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, 69500 Bron, France; INRA, USC1361, 69500 Bron, France; Embryology Unit, Children's Medical Research Institute, CMRI, Westmead, NSW 2145, Australia.
Pierre
P
Osteil
Univ Lyon, Université Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, 69500 Bron, France.
Anaïs
A
Moulin
Univ Lyon, Université Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, 69500 Bron, France.
Claire
C
Santamaria
ISARA-Lyon, 69007 Lyon, France; VetAgroSup, UPSP ICE, 69280 Marcy l'Etoile, France.
Thierry
T
Joly
UMR BDR, INRA, ENVA, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Luc
L
Jouneau
Univ Lyon, Université Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, 69500 Bron, France.
Maxime
M
Aubry
Univ Lyon, Université Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, 69500 Bron, France.
Yann
Y
Tapponnier
UMR BDR, INRA, ENVA, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Catherine
C
Archilla
UMR BDR, INRA, ENVA, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Barbara
B
Schmaltz-Panneau
GABI, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Jérôme
J
Lecardonnel
INRA, UMR 444, Génétique Cellulaire, 31076 Toulouse, France; ENVT, 31076 Toulouse, France.
Harmonie
H
Barasc
INRA, UMR 444, Génétique Cellulaire, 31076 Toulouse, France; ENVT, 31076 Toulouse, France.
Nathalie
N
Mouney-Bonnet
INRA, UMR1388 Génétique, Physiologie et Systèmes d'Elevage, 31326 Castanet Tolosan, France.
Clémence
C
Genthon
INRA, UMR1388 Génétique, Physiologie et Systèmes d'Elevage, 31326 Castanet Tolosan, France.
Alain
A
Roulet
INRA, UMR1388 Génétique, Physiologie et Systèmes d'Elevage, 31326 Castanet Tolosan, France.
Cécile
C
Donnadieu
INRA, UMR1388 Génétique, Physiologie et Systèmes d'Elevage, 31326 Castanet Tolosan, France.
Hervé
H
Acloque
NARIC, Agricultural Biotechnology Institute, 2100 Gödöllo, Hungary.
Elen
E
Gocza
UMR BDR, INRA, ENVA, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Véronique
V
Duranthon
Univ Lyon, Université Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, 69500 Bron, France; INRA, USC1361, 69500 Bron, France. Electronic address: marielle.afanassieff@inserm.fr.
Marielle
M
Afanassieff
Univ Lyon, Université Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, 69500 Bron, France. Electronic address: pierre.savatier@inserm.fr.
Pierre
P
Savatier
D000818:Animals; D015415:Biomarkers; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques; D002453:Cell Cycle; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D000066673:Cell Self Renewal / Q000235:genetics; D002478:Cells, Cultured; D019295:Computational Biology / Q000379:methods; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D053612:Janus Kinases / Q000378:metabolism; D053605:Leukemia Inhibitory Factor / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics; D011817:Rabbits; D015398:Signal Transduction; D059467:Transcriptome
D015415:Biomarkers; D053605:Leukemia Inhibitory Factor; D035683:MicroRNAs; D053612:Janus Kinases