Characterization of a Biomimetic Mesophase Composed of Nonionic Surfactants and an Aqueous Solvent.
We have investigated the physical and biomimetic properties of a sponge (L3) phase composed of pentaethylene glycol monododecyl ether (C12E5), a nonionic surfactant, an aqueous solvent, and a cosurfactant. The following cosurfactants, commonly used for solubilizing membrane proteins, were incorporated: n-octyl-β-d-glucopyranoside (β-OG), n-dodecyl-β-d-maltopyranoside (DDM), 4-cyclohexyl-1-butyl-β-d-maltoside (CYMAL-4), and 5-cyclohexyl-1-pentyl-β-d-maltoside (CYMAL-5). Partial phase diagrams of these systems were created. The L3phase was characterized using crossed polarizers, diffusion of a fluorescent probe by fluorescence recovery after pattern photobleaching (FRAPP), and freeze fracture electron microscopy (FFEM). By varying the hydration of the phase, we were able to tune the distance between adjacent bilayers. The characteristic distance (db) of the phase was obtained from small angle scattering (SAXS/SANS) as well as from FFEM, which yielded complementary dbvalues. These dbvalues were neither affected by the nature of the cosurfactant nor by the addition of membrane proteins. These findings illustrate that a biomimetic surfactant sponge phase can be created in the presence of several common membrane protein-solubilizing detergents, thus making it a versatile medium for membrane protein studies.
Laboratoire de Physique Statistique, Ecole Normale Supérieure, PSL Research University; Université Paris Diderot Sorbonne Paris Cité; Sorbonne Universités UPMC Univ Paris 06, CNRS, 24 rue Lhomond, 75005 Paris, France.|Univ Paris Descartes, Sorbonne Paris Cité. Laboratoire de Cristallographie et RMN Biologiques, CNRS UMR 8015, Paris, France.
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Adrien
Laboratoire de Physique Statistique, Ecole Normale Supérieure, PSL Research University; Université Paris Diderot Sorbonne Paris Cité; Sorbonne Universités UPMC Univ Paris 06, CNRS, 24 rue Lhomond, 75005 Paris, France.
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Rayan
Laboratoire de Physique Statistique, Ecole Normale Supérieure, PSL Research University; Université Paris Diderot Sorbonne Paris Cité; Sorbonne Universités UPMC Univ Paris 06, CNRS, 24 rue Lhomond, 75005 Paris, France.
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Reffay
Institut Laue-Langevin, 71 avenue des Martyrs, CS 20156, 38042 Grenoble Cedex 9, France.
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Porcar
Departamento de Física, Universidad de Sonora , Apdo Postal 1626, 83000 Hermosillo, Sonora Mexico.
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Maldonado
Univ Paris Descartes, Sorbonne Paris Cité. Laboratoire de Cristallographie et RMN Biologiques, CNRS UMR 8015, Paris, France.
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Ducruix
Laboratoire de Physique Statistique, Ecole Normale Supérieure, PSL Research University; Université Paris Diderot Sorbonne Paris Cité; Sorbonne Universités UPMC Univ Paris 06, CNRS, 24 rue Lhomond, 75005 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, INSERM, Laboratoire d'Imagerie Biomédicale, F-75006, Paris, France.
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Urbach
Sorbonne Universités, UPMC Univ Paris 06, CNRS, INSERM, Laboratoire d'Imagerie Biomédicale, F-75006, Paris, France.
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Taulier
D000473:Alkanes / Q000737:chemistry; D040761:Biomimetic Materials / Q000737:chemistry*; D004987:Ethers / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008567:Membranes, Artificial; D033363:Neutron Diffraction; D011092:Polyethylene Glycols / Q000737:chemistry*; D053838:Scattering, Small Angle; D012997:Solvents / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*; D013696:Temperature; D014783:Viscosity; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction
D000473:Alkanes; D004987:Ethers; D006027:Glycosides; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water; C007548:n-dodecane; C077053:pentaethylene glycol monododecyl ether; D011092:Polyethylene Glycols