Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles.
The study was focused on assessment of antibacterial activity, cytotoxicity and immune compatibility of biogenic silver nanoparticles (AgNPs) synthesized from Streptomyces sp. NH28 strain. Nanoparticles were biosynthesized and characterized by UV-Vis spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, nanoparticle tracking analysis system and zeta potential. Antibacterial activity was tested against Gram-positive and Gram-negative bacteria; minimal inhibitory concentration was recorded. Cytotoxicity was estimated using L929 mouse fibroblasts via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. Biocompatibility of AgNPs was performed using THP1-XBlue™ cells. Biogenic AgNPs presented high antibacterial activity against all tested bacteria. Minimum inhibitory concentration of AgNPs against bacterial cells was found to be in range of 1.25-10 μg/mL. Silver nanoparticles did not show any harmful interaction to mouse fibroblast cell line, and no activation of nuclear factor kappa-light-chain-enhancer of activated B (NF-κB) cells was observed at concentration below 10 µg/mL. The half-maximal inhibitory concentration (IC50) value was established at 64.5 μg/mL. Biological synthesis of silver can be used as an effective system for formation of metal nanoparticles. Biosynthesized AgNPs can be used as an antibacterial agent, which can be safe for eukaryotic cells.
Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, Toruń, Poland.
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M
Składanowski
Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, Toruń, Poland. golinska@umk.pl.
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http://orcid.org/0000-0001-9154-8191
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Golinska
Gastroimmunology Lab., Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lódź, Banacha 12/16, Lodz, Poland.
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K
Rudnicka
Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, Toruń, Poland.
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H
Dahm
Nanobiotechnology Lab., Department of Biotechnology, S.G.B. Amravati University, Amravati, Maharashtra, India.
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M
Rai
D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology* / Q000494:pharmacology* / Q000633:toxicity; D001419:Bacteria / Q000187:drug effects*; D002460:Cell Line; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D008826:Microbial Sensitivity Tests; D012834:Silver / Q000276:immunology* / Q000494:pharmacology* / Q000633:toxicity; D017550:Spectroscopy, Fourier Transform Infrared
D000900:Anti-Bacterial Agents; D012834:Silver
Actinomycetes; Antibacterial activity; Biogenic silver nanoparticles; Cytotoxicity; NF-κB activation