[miR-181a promotes the proliferation and metastasis of osteosarcoma cells by targeting RASSF1A].
OBJECTIVE
To investigate the role of miR-181a in promoting the proliferation and metastasis of osteosarcoma cells by targeting RASSF1A.


METHODS
The level of miR-181a in 30 human osteosarcoma tissues and corresponding bone tissues was detected by real-time PCR, and the correlation between the level of miR-181a and clinicopathological characteristics of osteosarcoma was analyzed. Osteosarcoma cells MG-63 were transfected with chemically-synthesized miR-181a mimics and inhibitors, and the proliferation, migration and invasion of MG-63 cells were detected by MTT and Transwell assay. The specific binding ability of miR-181a to RASSF1A 3'-UTR was theoretically predicted and detected by the dual luciferase reporter gene assay.


RESULTS
The level of miR-181a in osteosarcoma tissues was statistically higher than that in the corresponding bone tissues (P<0.001). However, the level of miR-181a was not correlated with gender, age, tumor size and stage (P>0.05). MTT and Transwell assays showed that the growth rate, migration and invasion ability of MG-63 cells with up-regulation of miR-181a was significantly increased compared with negative control (P<0.05), while the growth rate, migration and invasion ability of MG-63 with down-regulated miR-181a was significantly decreased compared with negative control (P<0.05). Luciferase reporter gene assay showed that miR-181a targeted the 3'-UTR of RASSF1A and regulated the expression of RASSF1A.


CONCLUSIONS
MiR-181a promotes the proliferation and metastasis of osteosarcoma cells through specifically binding to RASSF1A 3'-UTR and subsequent down-regulation of RASSF1A.
Department of Orthopaedics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Xiaofang
X
Zang
Department of Orthopaedics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Qin
Q
Li
Department of Orthopaedics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Weiguo
W
Wang
Department of Orthopaedics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Yong
Y
Zhou
Department of Orthopaedics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Shijie
S
Chen
Department of Orthopaedics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Tao
T
Xiao
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D035683:MicroRNAs; D009362:Neoplasm Metastasis; D012516:Osteosarcoma; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D025521:Tumor Suppressor Proteins; D015854:Up-Regulation
D035683:MicroRNAs; C411762:RASSF1 protein, human; D025521:Tumor Suppressor Proteins