Vitamin D treatment attenuates 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis but not oxazolone-induced colitis.
Crohn's disease (CD) and ulcerative colitis (UC) have different immunological mechanisms, while both of them are potential targets of vitamin D treatment. In this study, we have tried to address the role of vitamin D in CD and UC using two mouse models. Mice of C57B6L were given vitamin D before the induction of colitis. Our results showed that vitamin D attenuated 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis but not oxazolone-induced colitis. Vitamin D could preserve the local histology, alleviate inflammation, suppress apoptosis, maintain tight junction function and decrease permeability. Interestingly, it has more of an effect on local structure preservation and inflammation inhibition in CD than in UC mice. Vitamin D blocked the increase of helper T-cell type 1 (Th1)- and helper T-cell type 17 (Th17)-related cytokines in TNBS-induced colitis. But the increase of helper T-cell type 2 (Th2)- and regulatory T cells (Treg)-related cytokines was augmented at the same time in oxazolone-induced colitis which counteracted each other. Our study helps elucidate the differential protective effects of vitamin D on CD and UC patients, as reported in literature.
Department of Pediatric Orthopedics, Shengjing Hospital of China Medical University, NO. 36 Sanhao Street, Shenyang, Liaoning 110004, P. R. China.|Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital of China Medical University, NO. 36 Sanhao Street, Shenyang, Liaoning 110004, P. R. China.
Tianjing
T
Liu
Department of Neonatology, Shengjing Hospital of China Medical University, NO. 36 Sanhao Street, Shenyang, Liaoning 110004, P. R. China.
Yongyan
Y
Shi
Institute of Metabolic Disease Research and Drug Development, China Medical University, 77 Puhe Road, Shenyang, Liaoning 110004, P. R. China.
Jie
J
Du
Institute of Metabolic Disease Research and Drug Development, China Medical University, 77 Puhe Road, Shenyang, Liaoning 110004, P. R. China.
Xin
X
Ge
Department of Gastroenterology, Shengjing Hospital of China Medical University, NO. 36 Sanhao Street, Shenyang, Liaoning 110004, P. R. China.
Xu
X
Teng
Department of Gastroenterology, Shengjing Hospital of China Medical University, NO. 36 Sanhao Street, Shenyang, Liaoning 110004, P. R. China.
Lu
L
Liu
Department of Pediatric Orthopedics, Shengjing Hospital of China Medical University, NO. 36 Sanhao Street, Shenyang, Liaoning 110004, P. R. China.
Enbo
E
Wang
Department of Pediatric Orthopedics, Shengjing Hospital of China Medical University, NO. 36 Sanhao Street, Shenyang, Liaoning 110004, P. R. China.|Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital of China Medical University, NO. 36 Sanhao Street, Shenyang, Liaoning 110004, P. R. China.
Qun
Q
Zhao
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D003424:Crohn Disease / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D007249:Inflammation / Q000188:drug therapy; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010081:Oxazolone / Q000009:adverse effects*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects*; D014807:Vitamin D / Q000627:therapeutic use*
D016207:Cytokines; D014807:Vitamin D; D010081:Oxazolone; D014302:Trinitrobenzenesulfonic Acid