Reduced immune response to polymeric micelles coating sialic acids.
Effects of sialic acid coatings on polymeric micelle consisting of poly(sarcosine)-block-poly(l-lactic acid) (Lactosome) in the aim of prevention of the accelerated blood clearance (ABC) phenomenon are studied. Two kinds of the sialic acid-presenting Lactosomes targeting the immunosuppressive receptors of Siglec-G and CD22 have been successfully prepared. Lactosome presenting 5-N-acetylneuraminic acid or 5-N-acetylneuraminyl-α(2→6)-galactosyl-β(1→4)-N-acetylglucosamine at the nanocarrier surface diminished the ABC phenomenon due to the reduction of the anti-poly(sarcosine) IgM production. Further, the sialic acid moieties could interact possibly with Siglec-E on immune cell to suppress phagocytosis of the opsonized nanocarriers.
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto daigaku-katsura, Nishikyo-ku, Kyoto 615-8510, Japan. Electronic address: ohmae@peptide.polym.kyoto-u.ac.jp.
Masashi
M
Ohmae
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto daigaku-katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Mei
M
Kojima
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto daigaku-katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Kenta
K
Mihara
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto daigaku-katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Yuji
Y
Yamazaki
Technology Research Laboratory, Shimadzu Corporation, 3-9-4 Hikari-dai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan.
Isao
I
Hara
Department of Experimental Therapeutics, Institute for Advancement of Clinical and Translational Science, Kyoto University Hospital, 53 Shogoin kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
Eri
E
Hara
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto daigaku-katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Shunsaku
S
Kimura
D000818:Animals; D002240:Carbohydrate Sequence; D004337:Drug Carriers; D004797:Enzyme-Linked Immunosorbent Assay; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008823:Micelles; D053758:Nanoparticles; D011108:Polymers / Q000737:chemistry*; D012794:Sialic Acids / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013601:T-Lymphocytes / Q000276:immunology
D004337:Drug Carriers; D008823:Micelles; D011108:Polymers; D012794:Sialic Acids
Accelerated blood clearance phenomenon; Lactosome; Nanocarriers; Sialic acid; Siglecs