Streptomyces lacrimifluminis sp. nov., a novel actinobacterium that produces antibacterial compounds, isolated from soil.
A novel actinobacterial strain, designated Z1027T, was isolated from a soil sample collected near the Tuotuo River, Qinghai-Tibet Plateau (China). The strain exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus. The taxonomic position of strain Z1027T was determined using a polyphasic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Streptomyces and formed a distinct phyletic line in the 16S rRNA gene tree, together with Streptomyces turgidiscabies ATCC 700248T (99.19 % similarity), Streptomyces graminilatus JL-6T (98.84 %) and Streptomyces reticuliscabiei CFBP 4531T (98.36 %). The genomic DNA G+C content of strain Z1027T was 74±1 mol%. The DNA-DNA relatedness values between strain Z1027T and Streptomyces turgidiscabies ATCC 700248T and Streptomyces reticuliscabiei CFBP 4531T were 38.5±0.4 and 26.2±1.2 %, respectively, both of them signiﬁcantly lower than 70 %. Chemotaxonomic data revealed that strain Z1027T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, ll-diaminopimelic acid as the diagnostic diamino acid and galactose as a whole-cell sugar. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatydilinositol and seven other unknown polar lipids were detected; iso-C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0 were the major fatty acids. On the basis of these genotypic and phenotypic data, it is proposed that isolate Z1027T (=CGMCC 4.7272T=JCM 31054T) should be classified as the type strain of a novel species of the genus Streptomyces,Streptomyces lacrimifluminis sp. nov.
State Key Laboratory of Cryospheric Sciences, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy Sciences, Lanzhou 730000, PR China.|Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province 730000, PR China.
Binglin
B
Zhang
The Key Laboratory for Microbial Resources of Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Shukun
S
Tang
Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province 730000, PR China.|Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, PR China.
Ximing
X
Chen
Xinjiang Institute of Ecology and Geography, Chinese Academy of Science, Urumqi, PR China.
Ling
L
Zhang
Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province 730000, PR China.|Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, PR China.
Gaoseng
G
Zhang
Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province 730000, PR China.|Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, PR China.
Wei
W
Zhang
Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province 730000, PR China.|Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, PR China.
Guangxiu
G
Liu
State Key Laboratory of Cryospheric Sciences, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy Sciences, Lanzhou 730000, PR China.|Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province 730000, PR China.
Tuo
T
Chen
Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province 730000, PR China.|School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou 730070, PR China.
Shiweng
S
Li
Institute of Life Science, Swansea University Medical School, Singleton Park, Swansea SA2 8PP, UK.
Paul
P
Dyson
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000898:Antibiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid