HLA Class II with Lupus Nephritis in Moroccan Patients.
Lupus nephritis (LN) is a disease with a poor prognosis. The association between LN and the Human leukocyte antigen (HLA) genes has never been studied on a Moroccan population. The aim of this work was to evaluate the distribution of the HLA class II alleles in patients with LN and to determine susceptible and protective HLA alleles/haplotypes in LN. The association between these alleles, disease severity of LN, and age at onset were also investigated. Seventy-five patients with LN were compared with 169 healthy unrelated controls. HLA class II alleles typing was performed by polymerase chain reaction-sequence-specific primers (PCR-SSP). A significant increase of HLA-DRB1*15 allele frequency (p = 0.001) and a significant decrease of the HLA-DRB1*04 allele (p = 0.04) were observed in LN patients. The frequency of HLA-DRB1*15-DQB1*06 haplotype (p = 0.003) was increased in the patients while that of HLA-DRB1*04-DQB1*03 (p = 0.027) was decreased. A significant increase of HLA-DRB1*15 allele frequency (p = 0.0001) and HLA-DRB1*15-DQB1*06 haplotype (p = 0.002) was observed in patients with class IV LN. In the Moroccan population we demonstrated the positive association of HLA class II alleles and haplotypes with LN and with a severe form of nephritis. HLA-DRB1*15 allele does not determine the age of disease onset in LN.
a UPR of Immunology, Faculty of Medicine and Pharmacy , University Mohamed V , Rabat , Morocco.
Ouahiba
O
Bhallil
b Department of Nephrology , Dialysis and Kidney Transplantation , CHU Ibn Sina, Rabat , Morocco.
Aicha
A
Ibrahimi
c Department of Immunology and Transfusion , CHU Ibn Sina, Rabat , Morocco.
Sanae
S
Ouadghiri
b Department of Nephrology , Dialysis and Kidney Transplantation , CHU Ibn Sina, Rabat , Morocco.
Naima
N
Ouzeddoun
a UPR of Immunology, Faculty of Medicine and Pharmacy , University Mohamed V , Rabat , Morocco.|c Department of Immunology and Transfusion , CHU Ibn Sina, Rabat , Morocco.
Nadia
N
Benseffaj
b Department of Nephrology , Dialysis and Kidney Transplantation , CHU Ibn Sina, Rabat , Morocco.
Rabia
R
Bayahia
a UPR of Immunology, Faculty of Medicine and Pharmacy , University Mohamed V , Rabat , Morocco.|c Department of Immunology and Transfusion , CHU Ibn Sina, Rabat , Morocco.
Malika
M
Essakalli
D000328:Adult; D017668:Age of Onset; D018450:Disease Progression; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D059866:HLA-DQ beta-Chains / Q000235:genetics*; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006801:Humans; D007668:Kidney / Q000473:pathology*; D008181:Lupus Nephritis / Q000453:epidemiology / Q000235:genetics*; D009018:Morocco; D055815:Young Adult
D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D059811:HLA-DRB1 Chains; C527195:HLA-DRB1*04 antigen; C507799:HLA-DRB1*15 antigen
Human leukocyte antigen; Moroccan population; lupus nephritis