Immune response of newborn BALB/c mice to Cryptosporidium infection.
Cryptosporidium parvum is a protozoan parasite which causes diarrheal in human and animals worldwide. Infection transmission has reported through oral-fecal by infectious objects through foods and drinks. In this study we explored the immune response pathway in animal model for C. parvum to develop the new treatment way. Oocysts collected from fecal positive for C. parvum and diluted about 1:5 in sucrose solution. New born BALB/c mice (3 days) divided to 2 different groups. Control group hadn't received any oocyst, the test groups received 5 × 10(5) oocysts. 5 mice selected for each control group and 11 mice chosen for each test group. Blood collected from heart bleeds in days of 6, 9, 12 and 16. Protein concentrations determined by bio-photometer. Dot blotting used to find out total antibody concentrations oocyst antigen. Among the test and the control groups, blots appeared in test group which means antibody production, but not any blot observed in the control groups. The non-characteristic proteins in serum were measured by the biophotometer. In this study, we investigated antibody serum production against C. parvum oocysts in new born BALB/c mice. The detected antibody through dot blot technique was our aims which had conjugated to our characteristic antiserum. The recorded numbers for the controls by biophotometer related to the non characteristic proteins in serum. The results of this study can used to produce polyclonal or monoclonal antibodies against cryptosporidiosis.
Department of Medical Parasitology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Nasser
N
Ahmadian
Faculty of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Roghiyeh
R
Pashaei-Asl
Department of Medical Parasitology, Tarbiat Modarres University, Tehran, Iran.
Masomeh
M
Ahmadian
Department of Clinical Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Mohammad
M
Rahmati-Yamchi
Department of Medical Parasitology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Saed
S
Shahabi
Department of Nursing, Hamedan Branch, Islamic Azad University, Hamedan, Iran.
Hossein
H
Vazini
BALB/c mice; Cryptosporidium parvum; Immune response