Biodegradation-Resistant Multilayers Coated with Gold Nanoparticles. Toward a Tailor-made Artificial Extracellular Matrix.
Polymer multicomponent coatings such as multilayers mimic an extracellular matrix (ECM) that attracts significant attention for the use of the multilayers as functional supports for advanced cell culture and tissue engineering. Herein, biodegradation and molecular transport in hyaluronan/polylysine multilayers coated with gold nanoparticles were described. Nanoparticle coating acts as a semipermeable barrier that governs molecular transport into/from the multilayers and makes them biodegradation-resistant. Model protein lysozyme (mimics of ECM-soluble signals) diffuses into the multilayers as fast- and slow-diffusing populations existing in an equilibrium. Such a composite system may have high potential to be exploited as degradation-resistant drug-delivery platforms suitable for cell-based applications.
Fraunhofer Institute for Cell Therapy and Immunology , Am Mühlenberg 13, 14476 Potsdam-Golm, Germany.|Institute for Biochemistry and Biology, University of Potsdam , Maulbeerallee 2, 14469 Potsdam, Germany.
Vladimir Z
VZ
Prokopović
Fraunhofer Institute for Cell Therapy and Immunology , Am Mühlenberg 13, 14476 Potsdam-Golm, Germany.|School of Science and Technology, Nottingham Trent University , Clifton Lane, Nottingham NG11 8NS, United Kingdom.
Anna S
AS
Vikulina
Fraunhofer Institute for Cell Therapy and Immunology , Am Mühlenberg 13, 14476 Potsdam-Golm, Germany.|Institute for Biochemistry and Biology, University of Potsdam , Maulbeerallee 2, 14469 Potsdam, Germany.
David
D
Sustr
Fraunhofer Institute for Cell Therapy and Immunology , Am Mühlenberg 13, 14476 Potsdam-Golm, Germany.
Claus
C
Duschl
Fraunhofer Institute for Cell Therapy and Immunology , Am Mühlenberg 13, 14476 Potsdam-Golm, Germany.|School of Science and Technology, Nottingham Trent University , Clifton Lane, Nottingham NG11 8NS, United Kingdom.
Dmitry
D
Volodkin
cell adhesion; diffusion; enzymatic degradation; fluorescence recovery after photobleaching; hyaluronic acid; layer-by-layer; polylysine; semipermeable