Saccharomonospora xiaoerkulensis sp. nov., isolated from lake sediment.
A novel actinomycete, strain TRM 41495T, was isolated from lake sediment of Xiaoerkule lake in Xinjiang province, China, and was examined using a polyphasic approach. Strain TRM 41495T was aerobic, Gram-stain-positive, growing best on ISP medium 7 with abundant white aerial mycelium formed and brown soluble pigments produced. The optimum NaCl concentration for growth was 3.0 % (w/v), and the optimum temperature for growth was 37 °C. On the basis of 16S rRNA gene sequence analysis, strain TRM 41495T was shown to belong to the genus Saccharomonospora with a sequence similarity of 97.50 % with the most closely related species, Saccharomonospora xinjiangensis. The organism had chemical and morphological features typical of the genus Saccharomonospora. The cell wall of strain TRM 41495T contained meso-diaminopimelic acid, and galactose, arabinose, ribose and mannose were the major whole-cell sugars. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and an unknown phosphoglycolipid. The predominant menaquinones were MK-9(H4) and MK-9(H2). The major fatty acids were iso-C16 : 0, C16 : 0, C17 : 1ω6c, anteiso-C17 : 0 and iso-C16 : 1H. The G+C content of the DNA was 72.9 mol%. However, DNA-DNA hybridization studies between strain TRM 41495T and Saccharomonospora xinjiangensis showed only 43.9 % relatedness. Whole-cell sugars, menaquinones and fatty acids differentiate strain TRM 41495T from Saccharomonospora xinjiangensis. On the basis of the polyphasic evidence, strain TRM 41495T represents a novel species of the genus Saccharomonospora, for which the name Saccharomonospora xiaoerkulensis sp. nov. is proposed. The type strain is TRM 41495T (=CCTCC AA 2015038T=KCTC 39727T).
College of Life Science, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, PR China.
Dong
D
Li
College of Life Science, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, PR China.
Zheng-Jun
ZJ
Chen
College of Life Science, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, PR China.
Xiao-Xia
XX
Luo
College of Life Science, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, PR China.
Zhan-Feng
ZF
Xia
College of Life Science, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, PR China.
Chuan-Xing
CX
Wan
College of Life Science, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, PR China.
Li-Li
LL
Zhang
D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D060106:Lakes / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid