Specific Metabolome Profile of Exhaled Breath Condensate in Patients with Shock and Respiratory Failure: A Pilot Study.
BACKGROUND
Shock includes different pathophysiological mechanisms not fully understood and remains a challenge to manage. Exhaled breath condensate (EBC) may contain relevant biomarkers that could help us make an early diagnosis or better understand the metabolic perturbations resulting from this pathological situation.


OBJECTIVE
we aimed to establish the metabolomics signature of EBC from patients in shock with acute respiratory failure in a pilot study.


METHODS
We explored the metabolic signature of EBC in 12 patients with shock compared to 14 controls using LC-HRMS. We used a non-targeted approach, and we performed a multivariate analysis based on Orthogonal Partial Least Square-Discriminant Analysis (OPLS-DA) to differentiate between the two groups of patients.


RESULTS
We optimized the procedure of EBC collection and LC-HRMS detected more than 1000 ions in this fluid. The optimization of multivariate models led to an excellent model of differentiation for both groups (Q2 > 0.4) after inclusion of only 6 ions.


CONCLUSIONS
We validated the procedure of EBC collection and we showed that the metabolome profile of EBC may be relevant in characterizing patients with shock. We performed well in distinguishing these patients from controls, and the identification of relevant compounds may be promising for ICC patients.
Department of Anesthesiology and Intensive Care, CHRU Tours Bretonneau, 2 boulevard Tonnellé, 37044 Tours cedex 9, France. brice.fermier@gmail.com.
Brice
B
Fermier
Laboratoire de Biochimie et Biologie Moléculaire, CHRU Bretonneau, 2, boulevard Tonnellé, 37044 Tours cedex 9, France. helene.blasco@univ-tours.fr.|INSERM U930, équipe Neurogenetics and Neurometabolomics, Université François Rabelais, 10 bd Tonnellé, 37000 Tours, France. helene.blasco@univ-tours.fr.
Hélène
H
Blasco
Department of Anesthesiology and Intensive Care, CHRU Tours Bretonneau, 2 boulevard Tonnellé, 37044 Tours cedex 9, France. emmanuel.godat@gmail.com.
Emmanuel
E
Godat
PPF, Université François Rabelais, 10 bd tonnellé, 37000 Tours, France. cinzia.bocca@gmail.com.
Cinzia
C
Bocca
Department of Anesthesiology and Intensive Care, CHRU Tours Bretonneau, 2 boulevard Tonnellé, 37044 Tours cedex 9, France. joseph.moenne-loccoz@univ-tours.fr.
Joseph
J
Moënne-Loccoz
INSERM U930, équipe Neurogenetics and Neurometabolomics, Université François Rabelais, 10 bd Tonnellé, 37000 Tours, France. patrick.emond@univ-tours.fr.|PPF, Université François Rabelais, 10 bd tonnellé, 37000 Tours, France. patrick.emond@univ-tours.fr.
Patrick
P
Emond
Laboratoire de Biochimie et Biologie Moléculaire, CHRU Bretonneau, 2, boulevard Tonnellé, 37044 Tours cedex 9, France. christian.andres@univ-tours.fr.|INSERM U930, équipe Neurogenetics and Neurometabolomics, Université François Rabelais, 10 bd Tonnellé, 37000 Tours, France. christian.andres@univ-tours.fr.
Christian R
CR
Andres
Department of Anesthesiology and Intensive Care, CHRU Tours Bretonneau, 2 boulevard Tonnellé, 37044 Tours cedex 9, France. marc.laffon@univ-tours.fr.
Marc
M
Laffon
Department of Anesthesiology and Intensive Care, CHRU Tours Bretonneau, 2 boulevard Tonnellé, 37044 Tours cedex 9, France. martine.ferrandiere@univ-tours.fr.
Martine
M
Ferrandière
biomarkers; exhaled breath condensate; mass spectrometry; metabolomics; shock