Cytotoxic and Anti-inflammatory Sesquiterpenes from Ainsliaea henryi.
Three new sesquiterpenoids, 4α-hydroxyeudesm-11(13)-en-12-yl 3-methylbutanoate (1), diaspanolide E (2), and (13α)-germacra-1(10),4-dien-12,8α-olid-15-oic acid (3), along with eight known sesquiterpenoids (4 - 11), were isolated from the aerial parts of Ainsliaea henryi. The chemical structures of compounds 1 - 3 were elucidated by spectroscopic analysis (1D-, 2D-NMR, MS and HR/MS). All isolates were evaluated for their inhibitory activities against nitric oxide (NO) production in lipopolysaccharide-induced RAW264.7 macrophage cells. Compound 10 exhibited significantly inhibition against NO release with an IC50value of 6.54 ± 0.16 μm. Also, all isolated compounds were tested for cytotoxicity against three human tumor cell lines A549, MGC803, and HCT116, among which compound 5 significantly inhibited the proliferation of MGC803 cell lines with an IC50value of 2.2 ± 0.2 μm.
Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai, 200433, P. R. China.
Ren-Tao
RT
Zeng
Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai, 200433, P. R. China.
Xue-Yun
XY
Dong
Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai, 200433, P. R. China.
Xing
X
Fang
Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai, 200433, P. R. China.
Niao
N
Yang
School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, 210009, P. R. China.
Zhi-Ran
ZR
Shi
Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai, 200433, P. R. China.
Zhi-Guo
ZG
Zhuo
Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai, 200433, P. R. China.
Yun-Heng
YH
Shen
Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai, 200433, P. R. China.|Shanghai Institute of Pharmaceutical Industry, Shanghai, 200400, P. R. China.|School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, 210009, P. R. China.
Wei-Dong
WD
Zhang
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D010936:Plant Extracts; D012717:Sesquiterpenes; D009569:Nitric Oxide
Ainsliaea henryi; Ainsliaea species; Cytotoxic activities; NO production; Sesquiterpenoids