Unusual genomic features of a badnavirus infecting mulberry.
Mulberry badnavirus 1 (MBV1) has been characterized as the aetiological agent of a disease observed on a mulberry tree in Lebanon (accession L34). A small RNA next-generation sequencing library was prepared and analysed from L34 extract, and these data together with genome walking experiments have been used to obtain the full-length virus sequence. Uniquely among badnaviruses, the MBV1 sequence encodes a single ORF containing all the conserved pararetrovirus motifs. Two genome sizes (6 kb and 7 kb) were found to be encapsidated in infected plants, the shortest of which shares 98.95 % sequence identity with the full L34 genome. In the less-than-full-length deleted genome, the translational frame for the replication domains was conserved, but the particle morphology, observed under electron microscopy, was somehow altered. Southern blot hybridization confirmed the coexistence of the two genomic forms in the original L34 accession, as well as the absence of cointegration in the plant genome. Both long and deleted genomes were cloned and proved to be infectious in mulberry. Differently from other similar nuclear-replicating viruses or viroids, the characterization of the MBV1-derived small RNAs showed a reduced amount of the 24-mer class size.
Consiglio Nazionale delle Ricerche - Istituto per la Protezione Sostenibile delle Piante, Bari, Italy.
Michela
M
Chiumenti
Consiglio Nazionale delle Ricerche - Istituto per la Protezione Sostenibile delle Piante, Bari, Italy.
Massimiliano
M
Morelli
Consiglio Nazionale delle Ricerche - Istituto per la Protezione Sostenibile delle Piante, Bari, Italy.
Angelo
A
De Stradis
Istituto Agronomico Mediterraneo, Valenzano, Bari, Italy.
Toufic
T
Elbeaino
Consiglio Nazionale delle Ricerche - Istituto per la Protezione Sostenibile delle Piante, Bari, Italy.|International Institute of Tropical Agriculture, Ibadan, Nigeria.
Livia
L
Stavolone
Consiglio Nazionale delle Ricerche - Istituto per la Protezione Sostenibile delle Piante, Bari, Italy.
Angelantonio
A
Minafra
D000595:Amino Acid Sequence; D019195:Badnavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D016679:Genome, Viral; D023281:Genomics; D008969:Molecular Sequence Data; D031625:Morus / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D014764:Viral Proteins