Specific Anti-Leukemic Activity of the Peptide Warnericin RK and Analogues and Visualization of Their Effect on Cancer Cells by Chemical Raman Imaging.
Antimicrobial peptides can be used as therapeutic agents against cancer cells. Warnericin RK and derivatives (WarnG20D and WarnF14V) were tested on various, solid tumor or leukemia, cancer cells. These peptides appeared to be cytotoxic on all the cell types tested, cancerous as well healthy, but very interestingly displayed no deleterious effect on healthy mononuclear cells. The mode of action of the peptide was proposed to be membranolytic, using chemical Raman imaging. Addition of peptide induced a large disorganization of the membrane leading to the loss of the content of inner compartments of Jurkat cell, whereas no effect was observed on the healthy mononuclear cells. The less hemolytic peptides WarnG20D and WarnF14V could be good candidates for the leukemia treatment.
Laboratoire Ecologie & Biologie des Interactions, Equipe Microbiologie de l'Eau, Université de Poitiers, UMR CNRS 7267, Poitiers, France.
Clémence
C
Loiseau
Laboratoire Ecologie & Biologie des Interactions, Equipe Microbiologie de l'Eau, Université de Poitiers, UMR CNRS 7267, Poitiers, France.|Institut de Chimie Biologie des Membranes et Nanoobjets, CNRS-Université de Bordeaux, UMR 5248, Pessac, France.
Jacques
J
Augenstreich
Laboratoire Ecologie & Biologie des Interactions, Equipe Microbiologie de l'Eau, Université de Poitiers, UMR CNRS 7267, Poitiers, France.
Adrienne
A
Marchand
Institut de Chimie Biologie des Membranes et Nanoobjets, CNRS-Université de Bordeaux, UMR 5248, Pessac, France.
Etienne
E
Harté
Laboratoire Inflammation, Tissus Epithéliaux & Cytokines-EA 4331, Poitiers, France.
Martine
M
Garcia
Laboratoire Ecologie & Biologie des Interactions, Equipe Microbiologie de l'Eau, Université de Poitiers, UMR CNRS 7267, Poitiers, France.
Julien
J
Verdon
Signalisation & Transports Ioniques Membranaires, CNRS ERL-7368, Poitiers, France.
Marc
M
Mesnil
Institut de Chimie Biologie des Membranes et Nanoobjets, CNRS-Université de Bordeaux, UMR 5248, Pessac, France.
Sophie
S
Lecomte
Laboratoire Ecologie & Biologie des Interactions, Equipe Microbiologie de l'Eau, Université de Poitiers, UMR CNRS 7267, Poitiers, France.
Jean-Marc
JM
Berjeaud
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects; D001430:Bacteriocins / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D051379:Mice; D018613:Microscopy, Confocal; D009457:Neuroglia / Q000187:drug effects* / Q000473:pathology; D009928:Organ Specificity; D061251:Primary Cell Culture; D051381:Rats; D013059:Spectrum Analysis, Raman
D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; D001430:Bacteriocins; C558932:warnericin RK, Staphylococcus warneri