Novel strategy for sulfapyridine detection using a fully integrated electrochemical Bio-MEMS: Application to honey analysis.
Sulfapyridine (SPy) is a sulfonamide antibiotic largely employed as veterinary drugs for prophylactic and therapeutic purposes. Therefore, its spread in the food products has to be restricted. Herein, we report the synthesis and characterization of a novel electrochemical biosensor based on gold microelectrodes modified with a new structure of magnetic nanoparticles (MNPs) coated with poly(pyrrole-co-pyrrole-2-carboxylic acid) (Py/Py-COOH) for high efficient detection of SPy. This analyte was quantified through a competitive detection procedure with 5-[4-(amino)phenylsulfonamide]-5-oxopentanoic acid-BSA (SA2-BSA) antigens toward polyclonal antibody (Ab-155). Initially, gold working electrodes (WEs) of integrated biomicro electro-mechanical system (BioMEMS) were functionalized by Ppy-COOH/MNPs, using a chronoamperometric (CA) electrodeposition. Afterward, SA2-BSA was covalently bonded to Py/Py-COOH/MNP modified gold WEs through amide bonding. The competitive detection of the analyte was made by a mixture of a fixed concentration of Ab-155 and decreasing concentrations of SPy from 50µgL-1to 2ngL-1. Atomic Force Microscopy characterization was performed in order to ensure Ppy-COOH/MNPs electrodeposition on the microelectrode surfaces. Electrochemical measurements of SPy detection were carried out using electrochemical impedance spectroscopy (EIS). This biosensor was found to be highly sensitive and specific for SPy, with a limit of detection of 0.4ngL-1. This technique was exploited to detect SPy in honey samples by using the standard addition method. The measurements were highly reproducible for detection and interferences namely, sulfadiazine (SDz), sulfathiazole (STz) and sulfamerazine (SMz). Taking these advantages of sensitivity, specificity, and low cost, our system provides a new horizon for development of advanced immunoassays in industrial food control.
Biotechnology Agroalimentary and Biomedical Analysis Group, Department of Biology, Faculty of Sciences, Moulay Ismaïl University, BP 11201 Zitoune, Meknes, Morocco; Sensor Electronic and Instrumentation Group, Department of Physics, Faculty of Sciences, Moulay Ismail University, BP 11201 Zitoune, Meknes, Morocco; Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, Université Lyon 1, ENS Lyon -5, rue de la Doua, F-69100 Villeurbanne, France.
Nadia El Alami El
NEAE
Hassani
Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, Université Lyon 1, ENS Lyon -5, rue de la Doua, F-69100 Villeurbanne, France.
Abdoullatif
A
Baraket
Université de Lyon, LAGEP, UMR-5007, CNRS, Université Lyon 1, 5007, 43 Bd 11 Novembre 1918, F-69622 Villeurbanne, France.
Ernandes Taveira Tenório
ETT
Neto
Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, Université Lyon 1, ENS Lyon -5, rue de la Doua, F-69100 Villeurbanne, France.
Michael
M
Lee
Nanobiotechnology for Diagnostics (Nb4D), CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain; Nanobiotechnology for Diagnostics (Nb4D), Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain.
J-Pablo
JP
Salvador
Nanobiotechnology for Diagnostics (Nb4D), CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain; Nanobiotechnology for Diagnostics (Nb4D), Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain.
M-Pilar
MP
Marco
Instituto de Microelectronica de Barcelona, IMB-CNM (CSIC) Campus UAB, 08193 Bellaterra, Barcelona, Spain.
Joan
J
Bausells
Biotechnology Agroalimentary and Biomedical Analysis Group, Department of Biology, Faculty of Sciences, Moulay Ismaïl University, BP 11201 Zitoune, Meknes, Morocco.
Nezha El
NE
Bari
Sensor Electronic and Instrumentation Group, Department of Physics, Faculty of Sciences, Moulay Ismail University, BP 11201 Zitoune, Meknes, Morocco.
Benachir
B
Bouchikhi
Université de Lyon, LAGEP, UMR-5007, CNRS, Université Lyon 1, 5007, 43 Bd 11 Novembre 1918, F-69622 Villeurbanne, France.
Abdelhamid
A
Elaissari
Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, Université Lyon 1, ENS Lyon -5, rue de la Doua, F-69100 Villeurbanne, France.
Abdelhamid
A
Errachid
Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, Université Lyon 1, ENS Lyon -5, rue de la Doua, F-69100 Villeurbanne, France. Electronic address: nadia.zine@univ-lyon1.fr.
Nadia
N
Zine
D015374:Biosensing Techniques; D058266:Dielectric Spectroscopy; D005504:Food Analysis / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006722:Honey / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry; D018625:Microscopy, Atomic Force; D013427:Sulfapyridine / Q000737:chemistry / Q000302:isolation & purification*
D006046:Gold; D013427:Sulfapyridine
BioMEMS; Biosensor; Food control; Magnetic nanoparticles (MNP); Sulfapyridine