Ultrasonic Surgical Aspirate is a Reliable Source For Culturing Glioblastoma Stem Cells.
Glioma stem cells (GSCs) are thought to be the source of tumor growth and therapy resistance. To understand the biology of GSCs, and target these tumors therapeutically, we need robust strategies for in vitro expansion of primary GSCs. To date, tumor core biopsies have been the main established source of GSCs. Since these samples are used for diagnostic purposes, the available tissue for cell culture and therapeutic targeting can be limited. In addition, a core biopsy is usually taken from one part of the tumor, thus would be unlikely to represent intra-tumor heterogeneity. To overcome these problems, tissue fragments from all over the tumor can be collected using an ultrasonic aspirator during surgery, thus assembling a "global tumor biopsy". Usually, this ultrasonic aspirate (UA) sample is considered as biological waste after operations. Here, we show that UA samples offer a large and reliable source of live cells. Similar to core biopsies, UA samples enriched for GSCs that differentiated into neural lineages, showed inter-individual variation of GSC markers, and induced tumors. Molecular profiling showed that UA samples cover tumor heterogeneity better than core biopsies. These results suggest that UA samples can be used to establish large scale cultures for therapeutic applications.
Norwegian Center for Stem Cell Research, Department of Immunology, Oslo University Hospital, 1112 Blindern 0317 OSLO, Norway.|Department of Molecular Medicine, Institute of Basic Medical Sciences, The Medical Faculty, University of Oslo, Oslo, Norway.
Jinan
J
Behnan
Norwegian Center for Stem Cell Research, Department of Immunology, Oslo University Hospital, 1112 Blindern 0317 OSLO, Norway.|Department of Molecular Medicine, Institute of Basic Medical Sciences, The Medical Faculty, University of Oslo, Oslo, Norway.
Biljana
B
Stangeland
Unit of Molecular Neuro-Oncology, Neurological Institute C. Besta, Milan, Italy.
Tiziana
T
Langella
Unit of Molecular Neuro-Oncology, Neurological Institute C. Besta, Milan, Italy.
Gaetano
G
Finocchiaro
Vilhelm Magnus Laboratory of Neurosurgical Research, Institute for Surgical Research, Oslo University Hospital, 0424 Oslo, Norway.
Wayne
W
Murrell
Norwegian Center for Stem Cell Research, Department of Immunology, Oslo University Hospital, 1112 Blindern 0317 OSLO, Norway.|Department of Molecular Medicine, Institute of Basic Medical Sciences, The Medical Faculty, University of Oslo, Oslo, Norway.
Jan E
JE
Brinchmann
D000818:Animals; D001707:Biopsy, Needle / Q000379:methods*; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D002454:Cell Differentiation / Q000235:genetics; D002470:Cell Survival; D004252:DNA Mutational Analysis; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D006801:Humans; D061705:Image-Guided Biopsy / Q000379:methods; D016513:Mice, SCID; D014411:Neoplastic Stem Cells / Q000473:pathology* / Q000502:physiology; D059467:Transcriptome; D014407:Tumor Cells, Cultured; D018084:Ultrasonography, Interventional / Q000379:methods; D023041:Xenograft Model Antitumor Assays