Extraction of polyphenols in Himanthalia elongata and determination by high performance liquid chromatography with diode array detector prior to its potential use against oxidative stress.
Several studies have demonstrated the antioxidant capacity of seaweeds, which can be used for the development of biopharmaceuticals with extensive medical application. Antioxidant therapies appear to attenuate the organic deterioration originated by an excessive oxidative stress, which could prevent the harmful effects of various injuries such as ischemia-reperfusion (I/R) among others. Marine brown seaweeds play a significant role, as they are the only organisms on earth producing phlorotannins, which are polyphenols that exhibit important biological activity. To ensure obtaining an extract with the greatest antioxidant activity, some variables that affect the extraction of polyphenols are optimized, including seaweed amount, type of solvent, and time and temperature of extraction. Subsequently, the total phenolic content (TPC) and the antioxidant activity have been determined. The optimized condition was obtained for 6g of seaweed, ethanol: water proportion of 60:40 and 2h/60°C, achieving 548.33mg AG/100g seaweed and 76% of antioxidant activity. The characterization of the extracted polyphenols was made by HPLC/DAD. 11 polyphenols were identified in the extract: Phloroglucinol, Gallic Acid, Catechin, Rutin, Gentisic Acid, Chlorogenic Acid, Caffeic Acid, Coumaric, Ferulic, Myricetin and Quercetin.
Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia "San Vicente Mártir", C/Guillem de Castro, 94, 46003, Valencia, Spain. Electronic address: mabelan@mail.ucv.es.
Mariola
M
Belda
Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia "San Vicente Mártir", C/Guillem de Castro, 94, 46003, Valencia, Spain.
Desiré
D
Sanchez
Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia "San Vicente Mártir", C/Guillem de Castro, 94, 46003, Valencia, Spain.
Elena
E
Bover
Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia "San Vicente Mártir", C/Guillem de Castro, 94, 46003, Valencia, Spain.
Beatriz
B
Prieto
Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia "San Vicente Mártir", C/Guillem de Castro, 94, 46003, Valencia, Spain.
Carolina
C
Padrón
Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia "San Vicente Mártir", C/Guillem de Castro, 94, 46003, Valencia, Spain.
Dolores
D
Cejalvo
Facultad de Medicina, Universidad de Valencia, Avda. Blasco Ibáñez, 13, 46010, Valencia, Spain.
Jose Miguel
JM
Lloris
D000975:Antioxidants / Q000494:pharmacology; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D016166:Free Radical Scavengers / Q000032:analysis; D057230:Limit of Detection; D018384:Oxidative Stress; D000459:Phaeophyceae / Q000737:chemistry*; D059808:Polyphenols / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012624:Seaweed / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D000975:Antioxidants; D016166:Free Radical Scavengers; D059808:Polyphenols
2,2-Diphenyl-1-picrylhydrazyl (DPPH); Antioxidant activity; Himanthalia elongata; Polyphenols; Reverse phase high performance liquid chromatography (RP-HPLC); Total phenolic content (TPC)