Catechins Variously Affect Activities of Conjugation Enzymes in Proliferating and Differentiated Caco-2 Cells.
The knowledge of processes in intestinal cells is essential, as most xenobiotics come into contact with the small intestine first. Caco-2 cells are human colorectal adenocarcinoma that once differentiated, exhibit enterocyte-like characteristics. Our study compares activities and expressions of important conjugation enzymes and their modulation by green tea extract (GTE) and epigallocatechin gallate (EGCG) using both proliferating (P) and differentiated (D) caco-2 cells. The mRNA levels of the main conjugation enzymes were significantly elevated after the differentiation of Caco-2 cells. However, no increase in conjugation enzymes' activities in differentiated cells was detected in comparison to proliferating ones. GTE/EGCG treatment did not affect the mRNA levels of any of the conjugation enzymes tested in either type of cells. Concerning conjugation enzymes activities, GTE/EGCG treatment elevated glutathione S-transferase (GST) activity by approx. 30% and inhibited catechol-O-methyltransferase (COMT) activity by approx. 20% in differentiated cells. On the other hand, GTE as well as EGCG treatment did not significantly affect the activities of conjugation enzymes in proliferating cells. Administration of GTE/EGCG mediated only mild changes of GST and COMT activities in enterocyte-like cells, indicating a low risk of GTE/EGCG interactions with concomitantly administered drugs. However, a considerable chemo-protective effect of GTE via the pronounced induction of detoxifying enzymes cannot be expected as well.
Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Hradec Králové CZ-50005, Czech Republic. lnenickk@faf.cuni.cz.
Kateřina
K
Lněničková
Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Hradec Králové CZ-50005, Czech Republic. prochae1@faf.cuni.cz.
Eliška
E
Procházková
Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Hradec Králové CZ-50005, Czech Republic. skaloval@faf.cuni.cz.
Lenka
L
Skálová
Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Hradec Králové CZ-50005, Czech Republic. matousp7@faf.cuni.cz.
Petra
P
Matoušková
Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Hradec Králové CZ-50005, Czech Republic. barth3aa@faf.cuni.cz.
Hana
H
Bártíková
Toxicogenomics Unit, Centre of Toxicology and Health Safety, National Institute of Public Health, Prague CZ-10042, Czech Republic. pavel.soucek@szu.cz.
Pavel
P
Souček
Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Hradec Králové CZ-50005, Czech Republic. szotakova@faf.cuni.cz.
Barbora
B
Szotáková
D018938:Caco-2 Cells; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D002394:Catechol O-Methyltransferase / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005982:Glutathione Transferase / Q000096:biosynthesis*; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D013662:Tea / Q000737:chemistry
D012333:RNA, Messenger; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate; D002394:Catechol O-Methyltransferase; D005982:Glutathione Transferase
UDP-glucuronosyl transferase; cancer cells; catechol-O-methyltransferase; enterocyte-like cells; epigallocatechin gallate; glutathione S-transferase; green tea extract; sulfotransferase