Procyanidin Promotes Translocation of Glucose Transporter 4 in Muscle of Mice through Activation of Insulin and AMPK Signaling Pathways.
Procyanidins are the oligomeric or polymeric forms of epicatechin and catechin. In this study, we isolated and purified dimer to tetramer procyanidins from black soybean seed coat and investigated the anti-hyperglycemic effects by focusing on glucose transporter 4 (GLUT4) translocation and the underlying molecular mechanism in skeletal muscle of mice. The anti-hyperglycemic effects of procyanidins were also compared with those of monomer (-)-epicatechin (EC) and major anthocyanin, cyanidin-3-O-β-glucoside (C3G). To investigate GLUT4 translocation and its related signaling pathways, ICR mice were orally given procyanidins, EC and C3G in water at 10 μg/kg body weight. The mice were sacrificed 60 min after the dose of polyphenols, and soleus muscle was extracted from the hind legs. The results showed that trimeric and tetrameric procyanidins activated both insulin- and AMPK-signaling pathways to induce GLUT4 translocation in muscle of ICR mice. We confirmed that procyanidins suppressed acute hyperglycemia with an oral glucose tolerance test in a dose-dependent manner. Of these beneficial effects, cinnamtannin A2, one of the tetramers, was the most effective. In conclusion, procyanidins, especially cinnamtannin A2, significantly ameliorate postprandial hyperglycemia at least in part by promoting GLUT4 translocation to the plasma membrane by activating both insulin- and AMPK-signaling pathways.
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657-8501, Japan.
Yoko
Y
Yamashita
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657-8501, Japan.
Liuqing
L
Wang
Fujicco Co. Ltd, Research Development, Kobe, Hyogo 650-8558, Japan.
Fumio
F
Nanba
Fujicco Co. Ltd, Research Development, Kobe, Hyogo 650-8558, Japan.
Chiaki
C
Ito
Fujicco Co. Ltd, Research Development, Kobe, Hyogo 650-8558, Japan.
Toshiya
T
Toda
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657-8501, Japan.
Hitoshi
H
Ashida
D055372:AMP-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D000872:Anthocyanins / Q000008:administration & dosage; D044946:Biflavonoids / Q000008:administration & dosage*; D002392:Catechin / Q000008:administration & dosage*; D004195:Disease Models, Animal; D005947:Glucose / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000235:genetics* / Q000378:metabolism; D005960:Glucosides / Q000008:administration & dosage; D006801:Humans; D006943:Hyperglycemia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007328:Insulin / Q000378:metabolism*; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D044945:Proanthocyanidins / Q000008:administration & dosage*; D015398:Signal Transduction / Q000187:drug effects
D000872:Anthocyanins; D044946:Biflavonoids; D051275:Glucose Transporter Type 4; D005960:Glucosides; D007328:Insulin; D044945:Proanthocyanidins; C462279:cyanidin-3-O-beta-glucopyranoside; C017674:procyanidin; D002392:Catechin; D055372:AMP-Activated Protein Kinases; D005947:Glucose