Genome-wide characterization and expression analysis of MYB transcription factors in Gossypium hirsutum.
MYB family proteins are one of the most abundant transcription factors in the cotton plant and play diverse roles in cotton growth and evolution. Previously, few studies have been conducted in upland cotton, Gossypium hirsutum. The recent release of the G. hirsutum genome sequence provides a great opportunity to identify and characterize the entire upland cotton MYB protein family.



In this study, we undertook a comprehensive genome-wide characterization and expression analysis of the MYB transcription factor family during cotton fiber development. A total of 524 non-redundant cotton MYB genes, among 1986 MYB and MYB-related putative proteins, were identified and classified into four subfamilies including 1R-MYB, 2R-MYB, 3R-MYB, and 4R-MYB. Based on phylogenetic tree analysis, MYB transcription factors were divided into 16 subgroups. The results showed that the majority (69.1 %) of GhMYBs genes belong to the 2R-MYB subfamily in upland cotton.



Our comparative genomics analysis has provided novel insights into the roles of MYB transcription factors in cotton fiber development. These results provide the basis for a greater understanding of MYB regulatory networks and to develop new approaches to improve cotton fiber development.
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS), Anyang, 455000, China.|College of Life Sciences, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.|Zalingei University, Central Darfur, Sudan.
Haron
H
Salih
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS), Anyang, 455000, China.
Wenfang
W
Gong
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS), Anyang, 455000, China.
Shoupu
S
He
Department of Computer Science and Information Engineering, Anyang Institute of Technology, Anyang, China.
Gaofei
G
Sun
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS), Anyang, 455000, China. sunjl000@163.com.
Junling
J
Sun
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS), Anyang, 455000, China. dujeffrey8848@hotmail.com.
Xiongming
X
Du
D002874:Chromosome Mapping; D019295:Computational Biology / Q000379:methods; D017124:Conserved Sequence; D032561:Cotton Fiber; D030561:Databases, Nucleic Acid; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D056726:Genetic Association Studies; D018745:Genome, Plant; D055106:Genome-Wide Association Study; D023281:Genomics / Q000379:methods; D003368:Gossypium / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D059372:Nucleotide Motifs; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020598:Proto-Oncogene Proteins c-myb / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D010940:Plant Proteins; D020598:Proto-Oncogene Proteins c-myb; D014157:Transcription Factors
Comparative genomics analysis; Fiber development; MYB genes; Upland cotton