Development of novel biocompatible hybrid nanocomposites based on polyurethane-silica prepared by sol gel process.
Due to high biocompatibility, polyurethane has found many applications, particularly in development of biomedical devices. A new nanocomposite based on thermoset polyurethane and silica nanoparticles was synthesized using sol-gel method. Sol-gel process was fulfilled in two acidic and basic conditions by using tetraethylorthosilicate (TEOS) and trimethoxyisocyanatesilane as precursors. The hybrid films characterized for mechanical and surface properties using tensile strength, contact angle, ATR-FTIR and scanning electron microscopy. Biocompatibility and cytotoxicity of the hybrids were assessed using standard MTT, LDH and TUNEL assays. The results revealed that incorporation of silica nanoparticles was significantly improved tensile strength and mechanical properties of the hybrids. Based on the contact angle results, silica nanoparticles increased hydrophilicity of the hybrids. Biocompatibility by using human lung epithelial cell line (MRC-5) demonstrated that the hybrids were significantly less cytotoxic compared to pristine polymer as tested by MTT and LDH assays. TUNEL assay revealed no signs of apoptosis in all tested samples. The results of this study demonstrated that incorporation of silica nanoparticles into polyurethane lead to the enhancement of biocompatibility, indicating that these hybrids could potentially be used in biomedical field in particular as a new coating for medical implants.
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Ali
A
Rashti
Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, Tehran, Iran.
Hossein
H
Yahyaei
Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Saman
S
Firoozi
Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Sara
S
Ramezani
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Ali
A
Rahiminejad
Department of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Roya
R
Karimi
Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
Khadijeh
K
Farzaneh
Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, Tehran, Iran.
Mohsen
M
Mohseni
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran; Medical Biomaterials Research Center, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: hghanbari@tums.ac.ir.
Hossein
H
Ghanbari
D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055119:Elastic Modulus / Q000187:drug effects; D006801:Humans; D020287:In Situ Nick-End Labeling; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008422:Materials Testing / Q000379:methods*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D044367:Phase Transition / Q000187:drug effects*; D011140:Polyurethanes / Q000494:pharmacology*; D012822:Silicon Dioxide / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013314:Stress, Mechanical; D013499:Surface Properties
D001672:Biocompatible Materials; D011140:Polyurethanes; D012822:Silicon Dioxide; D007770:L-Lactate Dehydrogenase
Biocompatibility; Nanocomposite; Polyurethane; Silica nanoparticle; Sol-gel