Enhanced bioactivity, biocompatibility and mechanical behavior of strontium substituted bioactive glasses.
Strontium contained biomaterials have been reported as a potential bioactive material for bone regeneration, as it reduces bone resorption and stimulates bone formation. In the present investigation, the bioactive glasses were designed to partially substitute SrO for SiO2 in Na2O-CaO-SrO-P2O5-SiO2 system. This work demonstrates that the substitution of SrO for SiO2 has got significant benefit than substitution for CaO in the bioactive glass. Bioactivity was assessed by the immersion of the samples in simulated body fluid for different intervals. The formation of hydroxy carbonate apatite layer was identified by X-ray diffractometry, scanning electron microscopy (SEM) and energy dispersive spectroscopy. The elastic modulus of the bioactive glasses was measured and found to increase with increasing SrO for SiO2. The blood compatibility of the samples was evaluated. In vitro cell culture studies of the samples were performed using human osteosarcoma U2-OS cell lines and found a significant improvement in cell viability and proliferation. The investigation showed enhancement in bioactivity, mechanical and biological properties of the strontia substituted for silica in glasses. Thus, these bioactive glasses would be highly potential for bone regeneration.
Department of Ceramic Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India. Electronic address: askumar.rs.cer11@iitbhu.ac.in.
Sampath Kumar
SK
Arepalli
Department of Ceramic Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India.
Himanshu
H
Tripathi
Immunobiology Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
Sumit Kumar
SK
Hira
Immunobiology Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
Partha Pratim
PP
Manna
Department of Ceramic Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India.
Ram
R
Pyare
Department of Ceramic Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India.
S P Singh
D001031:Apatites / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002254:Carbonates / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D055119:Elastic Modulus; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005898:Glass / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012822:Silicon Dioxide / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D013324:Strontium / Q000737:chemistry*; D014961:X-Ray Diffraction
D001031:Apatites; D001672:Biocompatible Materials; D002254:Carbonates; C413465:hydroxycarbonate apatite; D012822:Silicon Dioxide; D013324:Strontium
Bioactive glass; Bioactivity; Biocompatibility; Mechanical properties; Network connectivity; Strontium oxide