In vitro biocompatibility of titanium after plasma surface alloying with boron.
Recently, the effect of different sizes of precursor powders during surface plasma alloying modification on the properties of titanium surface was studied. In this work we show in vitro test results of the titanium (α-Ti) after plasma surface alloying with boron (B). Ti-B nanopowders with 2 and 10wt% B were deposited onto microcrystalline Ti substrate. The in vitro cytocompatibility of these biomaterials was evaluated and compared with a conventional microcrystalline Ti. During the studies, established cell line of human gingival fibroblasts and osteoblasts were cultured in the presence of tested materials, and its survival rate and proliferation activity were examined. For this purpose, MTT assay, flow cytometric and fluorescent microscopic evaluation were made. Biocompatibility tests carried out indicate that the Ti after plasma surface alloying with B could be a possible candidate for dental implants and other medicinal applications. Plasma alloying is a promising method for improving the properties of titanium, thus increasing the field of its applications.
Department of Immunology, Chair of Clinical Immunology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806 Poznan, Poland. Electronic address: markacz@ump.edu.pl.
Mariusz
M
Kaczmarek
Division Mother's and Child's Health, Poznan University of Medical Sciences, Polna 33, 60-535 Poznan, Poland.
Mieczysława U
MU
Jurczyk
Institute of Materials Science and Engineering, Poznan University of Technology, Jana Pawla II 24, 61-138 Poznan, Poland.
Andrzej
A
Miklaszewski
Department of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznan, Poland.
Anna
A
Paszel-Jaworska
Department of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznan, Poland.
Aleksandra
A
Romaniuk
Department of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355 Poznan, Poland.
Natalia
N
Lipińska
Department of Immunobiochemistry, Chair of Biology and Environmental Sciences, Poznan University of Medical Sciences, Rokietnicka 8, 60-806 Poznan, Poland.
Jakub
J
Żurawski
Department of Cell Biology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806 Poznan, Poland.
Paulina
P
Urbaniak
Department of Conservative Dentistry and Periodontology, Poznan University of Medical Sciences, Bukowska 70, 60-812 Poznan, Poland.
Karolina
K
Jurczyk
D000497:Alloys / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000494:pharmacology*; D001895:Boron / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003460:Crystallization; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005453:Fluorescence; D006801:Humans; D008422:Materials Testing / Q000379:methods*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D058626:Plasma Gases / Q000494:pharmacology*; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*; D014961:X-Ray Diffraction
D000497:Alloys; D001672:Biocompatible Materials; D058626:Plasma Gases; D014025:Titanium; D001895:Boron
Boron; Cell proliferation; Cell viability; Microplasma alloying; Nanoprecursors; Titanium