In Vitro Effects of Some Botanicals with Anti-Inflammatory and Antitoxic Activity.
Several extrinsic factors, like drugs and chemicals, can foster autoimmunity. Tetracyclines, in particular oxytetracycline (OTC), appear to correlate with the emergence of immune-mediated diseases. Accumulation of OTC, the elective drug for gastrointestinal and respiratory infectious disease treatment in broiler chickens, was reported in chicken edible tissues and could represent a potential risk for pets and humans that could assume this antibiotic as residue in meat or in meat-derived byproducts. We investigated the in vitro anti-inflammatory properties of a pool of thirteen botanicals as a part of a nutraceutical diet, with proven immunomodulatory activity. In addition, we evaluated the effect of such botanicals in contrasting the in vitro proinflammatory toxicity of OTC. Our results showed a significant reduction in interferon- (INF-) γ production by human and canine lymphocytes in presence of botanicals ((⁎) p < 0.05). Increased INF-γ production, dependent on 24-hour OTC-incubation of T lymphocytes, was significantly reduced by the coincubation with Haematococcus pluvialis, with Glycine max, and with the mix of all botanicals ((⁎) p < 0.05). In conclusion, the use of these botanicals was shown to be able to contrast OTC-toxicity and could represent a new approach for the development of functional foods useful to enhance the standard pharmacological treatment in infections as well as in preventing or reducing the emergence of inflammatory diseases.
Division of Research and Development, SANYpet SpA, 35023 Bagnoli di Sopra, Italy.
Gianandrea
G
Guidetti
School of Specialization in Clinical Biochemistry, "G. d'Annunzio" University, 66100 Chieti, Italy.
Alessandro
0000-0001-8122-9170
A
Di Cerbo
Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.
Angela
A
Giovazzino
Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.
Valentina
V
Rubino
Ph.D. School of Science, University of Basilicata, 85100 Potenza, Italy.
Anna Teresa
AT
Palatucci
Division of Research and Development, SANYpet SpA, 35023 Bagnoli di Sopra, Italy.
Sara
S
Centenaro
Division of Research and Development, SANYpet SpA, 35023 Bagnoli di Sopra, Italy.
Elena
E
Fraccaroli
Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80100 Naples, Italy.
Laura
L
Cortese
Department of Science, University of Basilicata, 85100 Potenza, Italy.
Maria Grazia
MG
Bonomo
Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.
Giuseppina
G
Ruggiero
Division of Research and Development, SANYpet SpA, 35023 Bagnoli di Sopra, Italy.
Sergio
S
Canello
Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy; Department of Science, University of Basilicata, 85100 Potenza, Italy.
Giuseppe
G
Terrazzano
D000328:Adult; D000821:Animal Feed; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002478:Cells, Cultured; D002645:Chickens; D003470:Culture Media / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D019587:Dietary Supplements; D004285:Dogs; D005260:Female; D055951:Functional Food; D006801:Humans; D056747:Immunomodulation / Q000187:drug effects; D007249:Inflammation / Q000517:prevention & control*; D007371:Interferon-gamma / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008297:Male; D010118:Oxytetracycline / Q000633:toxicity; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry
D000893:Anti-Inflammatory Agents; D003470:Culture Media; D016207:Cytokines; D010936:Plant Extracts; D007371:Interferon-gamma; D010118:Oxytetracycline