Isolation and analysis of vitamin B12 from plant samples.
Based on increased demands of strict vegetarians, an investigation of vitamin B12 content in plant sources, was carried out. The vitamin B12 concentration was determined by RP-HPLC with UV detection, after prior matrix isolation by immunoaffinity chromatography (IAC). Vitamin B12 was extracted in the presence of sodium cyanide, to transform all forms of cobalamin into cyanocobalamin. Diode array detector was used to monitor vitamin B12, after its chromatographic separation under gradient elution with a mobile phase consisting of acetonitrile and trifluoroacetic acid 0.025% (w/v). The method demonstrated excellent linearity with a limit of detection 0.004μg/ml. The method precision was evaluated for plant samples and it was below 0.7% (n=6). Significant amounts of vitamin B12 in plants were detected in Hippophae rhamnoides (37μg/100g dry weight), in Elymus (26μg/100g dry weight) and in Inula helenium (11μg/100g dry weight).
Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstrasse 5, 30167 Hanover, Germany. Electronic address: michailnak@hotmail.com.
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Nakos
Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstrasse 5, 30167 Hanover, Germany. Electronic address: pepelanova@iftc.uni-hannover.de.
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Pepelanova
Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstrasse 5, 30167 Hanover, Germany. Electronic address: beutel@iftc.uni-hannover.de.
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Beutel
Institute of Food Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstrasse 5, 30167 Hanover, Germany. Electronic address: krings@lci.uni-hannover.de.
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Krings
Institute of Food Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstrasse 5, 30167 Hanover, Germany. Electronic address: rg.berger@lci.uni-hannover.de.
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Berger
Institute of Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstrasse 5, 30167 Hanover, Germany. Electronic address: scheper@iftc.uni-hannover.de.
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Scheper
D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031749:Elymus / Q000737:chemistry; D040161:Frankia / Q000737:chemistry; D031281:Hippophae / Q000737:chemistry; D014805:Vitamin B 12 / Q000032:analysis / Q000302:isolation & purification*; D014803:Vitamin B Complex / Q000032:analysis / Q000302:isolation & purification
D014803:Vitamin B Complex; D014805:Vitamin B 12
Frankia alni; HPLC-MS/MS; HPLC-UV; Hippophae rhamnoides; Immunoaffinity chromatography (IAC); Plant-based foods; Vitamin B(12) (cobalamin)