Superior antibacterial activity of GlcN-AuNP-GO by ultraviolet irradiation.
A complete bacterialysis analysis of glucosamine-gold nanoparticle-graphene oxide (GlcN-AuNP-GO) and UV-irradiated GlcN-AuNP-GO was conducted. Analytical characterization of GlcN-AuNPs, GO and GlcN-AuNP-GO revealed UV-Vis absorbance peak at around 230 and 500nm. Microscopic characterization of prepared nanomaterials was performed by scanning electron microscope, atomic force microscopy, and high-resolution transmission microscopy. The results confirmed that the GlcN-AuNPs were uniformly decorated on the surface and edges of graphene sheets. In addition, potent antibacterial activity of GlcN-AuNP-GO that was UV irradiated for 10min and normal GlcN-AuNP-GO was detected, compared to the standard drug kanamycin, against both Gram-negative and positive bacteria. The minimum inhibitory concentration (MIC) and fluorescence intensity spectra results for Escherichia coli and Enterococcus faecalis showed that the UV-irradiated GlcN-AuNP-GO has better antibacterial activity than normal GlcN-AuNP-GO and kanamycin. Morphological changes were detected by AFM after treatment. These results confirmed that GlcN-AuNP-GO is a potent antibacterial agent with good potential for use in manufacturing medical instruments, pharmaceutical industries and in waste water treatment.
Gachon Bionano Research Institute, Department of Bionanotechnology, Gachon University, Gyeonggi-do 13120, Republic of Korea.
Saravanan
S
Govindaraju
Gachon Bionano Research Institute, Department of Bionanotechnology, Gachon University, Gyeonggi-do 13120, Republic of Korea.
Monica
M
Samal
Gachon Bionano Research Institute, Department of Bionanotechnology, Gachon University, Gyeonggi-do 13120, Republic of Korea. Electronic address: ykyusik@gachon.ac.kr.
Kyusik
K
Yun
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005944:Glucosamine / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry; D014466:Ultraviolet Rays
D000900:Anti-Bacterial Agents; D010087:Oxides; D006046:Gold; D006108:Graphite; D005944:Glucosamine
Antibacterial activity; Glucosamine; Gold nanoparticles; Graphene oxide; Morphological study; Ultraviolet irradiation