Deinococcus persicinus sp. nov., a radiation-resistant bacterium from soil.
Two Gram-stain-negative, oxidase-negative, catalase-positive, aerobic and coccus-shaped bacterial strains, KSY3-6T and JSH6-18, were isolated from soil in South Korea. Strains KSY3-6T and JSH6-18 showed high resistance to gamma-ray and UVC irradiation. The 16S rRNA gene sequences of strains KSY3-6T and JSH6-18 showed a novel subline within the genus Deinococcus in the family Deinococcaceae. They shared 94.8-86.4 % nucleotide similarities with other species of the genus Deinococcus. Strain KSY3-6T exhibited high DNA-DNA hybridization values with JSH6-18 (77±0.8 %). The two strains showed typical chemotaxonomic characteristics of the genus Deinococcus, including the presence of menaquinone 8 (MK-8) as predominant respiratory quinone and C16 : 0, C17 : 0cyclo and summed feature 3 (C16 : 1ω7c/C16: 1ω6c) as major fatty acids. The G+C content of the DNA of strains KSY3-6T and JSH6-18 was 62.0 and 62.4 mol%, respectively. Polar lipids in strains KSY3-6T and JSH6-18 were mainly phosphoglycolipids. Based on their phenotypic and genotypic properties, strains KSY3-6T and JSH6-18 should be classified as representatives of a novel species in the genus Deinococcus, for which the name Deinococcus persicinus sp. nov. is proposed. The type strain is KSY3-6T (=KCTC 33787T=JCM 31313T). The reference strain is JSH6-18 (=KCTC 33788=JCM 31312).
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
Seon Hwa
SH
Jeon
Microorganism Resources Division, National Institute of Biological Resources, Incheon 404-107, Republic of Korea.
Myung-Suk
MS
Kang
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
Eun Sun
ES
Joo
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
Eun Bit
EB
Kim
Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea.
Sangyong
S
Lim
Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea.
Sun-Wook
SW
Jeong
College of Agricultural and Life Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.|Institute of Plant Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
Hee-Young
HY
Jung
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
Sathiyaraj
S
Srinivasan
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
Myung Kyum
MK
Kim
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D034301:Deinococcus / Q000145:classification* / Q000302:isolation & purification / Q000528:radiation effects; D005227:Fatty Acids / Q000737:chemistry; D005720:Gamma Rays; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014466:Ultraviolet Rays; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8