The preosteoblast response of electrospinning PLGA/PCL nanofibers: effects of biomimetic architecture and collagen I.
Constructing biomimetic structure and incorporating bioactive molecules is an effective strategy to achieve a more favorable cell response. To explore the effect of electrospinning (ES) nanofibrous architecture and collagen I (COL I)-incorporated modification on tuning osteoblast response, a resorbable membrane composed of poly(lactic-co-glycolic acid)/poly(caprolactone) (PLGA/PCL; 7:3 w/w) was developed via ES. COL I was blended into PLGA/PCL solution to prepare composite ES membrane. Notably, relatively better cell response was delivered by the bioactive ES-based membrane which was fabricated by modification of 3,4-dihydroxyphenylalanine and COL I. After investigation by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement, and mechanical test, polyporous three-dimensional nanofibrous structure with low tensile force and the successful integration of COL I was obtained by the ES method. Compared with traditional PLGA/PCL membrane, the surface hydrophilicity of collagen-incorporated membranes was largely enhanced. The behavior of mouse preosteoblast MC3T3-E1 cell infiltration and proliferation on membranes was studied at 24 and 48 hours. The negative control was fabricated by solvent casting. Evaluation of cell adhesion and morphology demonstrated that all the ES membranes were more favorable for promoting the cell adhesion and spreading than the casting membrane. Cell Counting Kit-8 assays revealed that biomimetic architecture, surface topography, and bioactive properties of membranes were favorable for cell growth. Analysis of β1 integrin expression level by immunofluorescence indicated that such biomimetic architecture, especially COL I-grafted surface, plays a key role in cell adhesion and proliferation. The real-time polymerase chain reaction suggested that both surface topography and bioactive properties could facilitate the cell adhesion. The combined effect of biomimetic architecture with enhanced surface activity by 3,4-dihydroxyphenylalanine-assisted modification and COL I incorporation of PLGA/PCL electrospun membranes could successfully fill osteogenic defects and allow for better cell proliferation and differentiation.
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing; Center of Stomatology, The Second Affiliated Hospital of Soochow University, Suzhou.
Yunzhu
Y
Qian
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing.
Hanbang
H
Chen
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing.
Yang
Y
Xu
Center of Stomatology, The Second Affiliated Hospital of Soochow University, Suzhou.
Jianxin
J
Yang
School of Biological Science and Medical Engineering, Southeast University, Nanjing, People's Republic of China.
Xuefeng
X
Zhou
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing.
Feimin
F
Zhang
School of Biological Science and Medical Engineering, Southeast University, Nanjing, People's Republic of China.
Ning
N
Gu
D000818:Animals; D001672:Biocompatible Materials; D032701:Biomimetics; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000737:chemistry*; D004295:Dihydroxyphenylalanine / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D019344:Lactic Acid / Q000737:chemistry*; D008567:Membranes, Artificial; D051379:Mice; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D011091:Polyesters / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry
D001672:Biocompatible Materials; D024042:Collagen Type I; D008567:Membranes, Artificial; D011091:Polyesters; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C016240:polycaprolactone; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004295:Dihydroxyphenylalanine
bioactive molecular; biomimetic architecture; collagen; guided bone regeneration; preosteoblast response