Optimization of polysaccharides extraction from watermelon rinds: Structure, functional and biological activities.
In the present work, optimization of hot water extraction, structural characteristics, functional properties, and biological activities of polysaccharides extracted from watermelon rinds (WMRP) were investigated. The physicochemical characteristics and the monosaccharide composition of these polysaccharides were then determined using chemical composition analysis, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM) and gas chromatography-flame ionization detection (GC-FID). SEM images showed that extracted polysaccharides had a rough surface with many cavities. GC-FID results proved that galactose was the dominant sugar in the extracted polysaccharides, followed by arabinose, glucose, galacturonic acid, rhamnose, mannose, xylose and traces of glucuronic acid. The findings revealed that WMRP displayed excellent antihypertensive and antioxidant activities. Those polysaccharides had also a protection effect against hydroxyl radical-induced DNA damage. Functional properties of extracted polysaccharides were also evaluated. WMRP showed good interfacial dose-dependent proprieties. Overall, the results suggested that WMRP presents a promising natural source of antioxidants and antihypertensive agents.
Enzymes & Bioconversion Unit, National School of Engineers, PB 1173, 3038 Sfax, Tunisia.
Molka Ben
MB
Romdhane
Enzymes & Bioconversion Unit, National School of Engineers, PB 1173, 3038 Sfax, Tunisia. Electronic address: anissa_ing@yahoo.fr.
Anissa
A
Haddar
Enzymes & Bioconversion Unit, National School of Engineers, PB 1173, 3038 Sfax, Tunisia.
Imen
I
Ghazala
Enzymes & Bioconversion Unit, National School of Engineers, PB 1173, 3038 Sfax, Tunisia.
Khawla Ben
KB
Jeddou
Service de Chromatographie Purification et analyse de polysaccharides CERMAV-CNRS, 601 rue de la Chimie, 38041 Grenoble cedex 9, France.
Claire Boisset
CB
Helbert
Enzymes & Bioconversion Unit, National School of Engineers, PB 1173, 3038 Sfax, Tunisia.
Semia
S
Ellouz-Chaabouni
D000959:Antihypertensive Agents; D000975:Antioxidants; D005591:Chemical Fractionation / Q000379:methods*; D029773:Citrullus / Q000737:chemistry*; D005410:Flame Ionization; D008855:Microscopy, Electron, Scanning; D009005:Monosaccharides; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared
D000959:Antihypertensive Agents; D000975:Antioxidants; D009005:Monosaccharides; D011134:Polysaccharides
ACE inhibitory activity; Antioxidant activities; Functional proprieties; Polysaccharides; Watermelon rinds