Poly I:C primes the suppressive function of human palatine tonsil-derived MSCs against Th17 differentiation by increasing PD-L1 expression.
It has been established that mesenchymal stem cells (MSCs) can have a suppressive effect on T cells, yet much remains unknown about the underlying mechanisms that support this effect. The T cell co-stimulatory pathway involving the programmed death-1 (PD-1) receptor and its ligand PD-L1 regulates T cell activation, tolerance, and subsequent immune-mediated tissue damage. In this study, human palatine tonsil-derived MSCs (T-MSCs) constitutively expressed PD-L1 and exhibited a suppressive activity that specifically targeted murine Th17 differentiation. Additionally, polyinosinic-polycytidylic acid (poly I:C), a Toll-like receptor 3 (TLR3) ligand, increased PD-L1 expression on T-MSCs. The elevated PD-L1 levels enhanced the suppressive functions of T-MSCs on Th17 differentiation. Therefore, pre-stimulation of T-MSCs with poly I:C may serve as an effective therapeutic priming step for modulating Th17-dominant immune responses.
Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea.
Kyung-Ah
KA
Cho
Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea.
Minhwa
M
Park
Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea.
Yu-Hee
YH
Kim
Department of Pediatrics, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea.
Kyung-Ha
KH
Ryu
Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea. Electronic address: soyounwoo@ewha.ac.kr.
So-Youn
SY
Woo
D060890:B7-H1 Antigen / Q000235:genetics*; D015415:Biomarkers; D002454:Cell Differentiation / Q000276:immunology*; D005786:Gene Expression Regulation; D006801:Humans; D056747:Immunomodulation; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000276:immunology; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000378:metabolism*; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology; D011070:Poly I-C / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D058504:Th17 Cells / Q000166:cytology* / Q000502:physiology*
D060890:B7-H1 Antigen; D015415:Biomarkers; C423236:CD274 protein, human; D011070:Poly I-C
PD-L1; Poly I:C; Th17; Tonsil-derived MSCs