Genotyping of Klebsiella Pneumonia Strains Isolated from Eldly Inpatients by Multiple-locus Variable-number Tandem-repeat Analysis.
Objective To investigate the genotype of klebsiella pneumonia strains isolated from eldly inpatients by multiple-locus variable-number tandem-repeat analysis. Methods Totally 184 klebsiella pneumonia strains,isolated from eldly inpatients,were collected,and their genome DNA were extracted. The polymorphism of 7 variable-number tandem-repeat locus in the DNA samples was analyzed by multiple primers polymerase chain reaction and capillary electrophoresis. The clustering analysis of genotyping was carried out with the BioNumerics 5.1 software. Results A total of 139 genotypes were identified in 184 klebsiella pneumonia clinical strains,showing obvious genetic polymorphisms. With clustering analysis of genotypes,all the strains were categorized into three gene clusters (genogroups 1,2,and 3). The genogroup 1 was the biggest cluster,containing 93.06% of the isolated strains. Conclusion There was a predominant cluster in the klebsiella pneumonia strains isolated from eldly inpatients in our center,and the major source of klebsiella pneumonia infection remained the nosocomial infection.
1. State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China; 2. Institute of Infectious Diseases,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China;
Yuan-Yuan
YY
Zhang
Department of Clinical Laboratory in South Building,Chinese PLA General Hospital,Beijing 100853,China.
Ya-Ping
YP
Xu
1. State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China; 2. Institute of Infectious Diseases,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China;
Peng-Cheng
PC
DU
State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China.
Yu-Jun
YJ
Qiang
State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China.
Wen
W
Zhang
Institute of Infectious Diseases,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China.
Chen
C
Chen
Department of Clinical Laboratory in South Building,Chinese PLA General Hospital,Beijing 100853,China.
Ji-Hong
JH
Yu
1. Department of Respiratory Medicine,Beijing Tsinghua Changgung Hospital,Medical Center,Tsinghua University,Beijing 102218,China; 2. Department of Geriatric Respiratory Medicine in South Building,Chinese PLA General Hospital,Beijing 100853,China;
Jun
J
Guo
D000368:Aged; D015373:Bacterial Typing Techniques; D003428:Cross Infection; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D007297:Inpatients; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000302:isolation & purification; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic