Fetal brain lesions after subcutaneous inoculation of Zika virus in a pregnant nonhuman primate.
We describe the development of fetal brain lesions after Zika virus (ZIKV) inoculation in a pregnant pigtail macaque. Periventricular lesions developed within 10 d and evolved asymmetrically in the occipital-parietal lobes. Fetal autopsy revealed ZIKV in the brain and significant cerebral white matter hypoplasia, periventricular white matter gliosis, and axonal and ependymal injury. Our observation of ZIKV-associated fetal brain lesions in a nonhuman primate provides a model for therapeutic evaluation.
Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA.
Kristina M
KM
Adams Waldorf
Department of Immunology, University of Washington, Seattle, Washington, USA.|Center for Innate Immunity and Immune Disease, University of Washington, Seattle, Washington, USA.
Jennifer E
JE
Stencel-Baerenwald
Department of Pathology, University of Washington, Seattle, Washington, USA.|Department of Pathology, Seattle Children's Hospital, Seattle, Washington, USA.
Raj P
RP
Kapur
Department of Pediatrics, University of Washington, Seattle, Washington, USA.|Department of Bioengineering, University of Washington, Seattle, Washington, USA.|Department of Radiology, University of Washington, Seattle, Washington, USA.
Colin
C
Studholme
Department of Pediatrics, University of Washington, Seattle, Washington, USA.|Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
Erica
E
Boldenow
Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.|Department of Global Health, University of Washington, Seattle, Washington, USA.
Jay
J
Vornhagen
Washington National Primate Research Center, Seattle, Washington, USA.
Audrey
A
Baldessari
Department of Radiology, University of Washington, Seattle, Washington, USA.
Manjiri K
MK
Dighe
Department of Radiology, University of Washington, Seattle, Washington, USA.
Jeff
J
Thiel
Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
Sean
S
Merillat
Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.|Department of Global Health, University of Washington, Seattle, Washington, USA.
Blair
B
Armistead
Department of Immunology, University of Washington, Seattle, Washington, USA.|Center for Innate Immunity and Immune Disease, University of Washington, Seattle, Washington, USA.
Jennifer
J
Tisoncik-Go
Department of Immunology, University of Washington, Seattle, Washington, USA.|Center for Innate Immunity and Immune Disease, University of Washington, Seattle, Washington, USA.
Richard R
RR
Green
Department of Immunology, University of Washington, Seattle, Washington, USA.|Center for Innate Immunity and Immune Disease, University of Washington, Seattle, Washington, USA.
Michael A
MA
Davis
Department of Immunology, University of Washington, Seattle, Washington, USA.|Center for Innate Immunity and Immune Disease, University of Washington, Seattle, Washington, USA.
Elyse C
EC
Dewey
Department of Immunology, University of Washington, Seattle, Washington, USA.|Center for Innate Immunity and Immune Disease, University of Washington, Seattle, Washington, USA.
Marian R
MR
Fairgrieve
Department of Radiology, University of Washington, Seattle, Washington, USA.
J Christopher
JC
Gatenby
Department of Radiology, University of Washington, Seattle, Washington, USA.
Todd
T
Richards
Department of Pathology, University of Washington, Seattle, Washington, USA.|Department of Neurology, University of Washington, Seattle, Washington, USA.
Gwenn A
GA
Garden
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.|Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA.|Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.|Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St. Louis, Missouri, USA.
Michael S
MS
Diamond
Department of Pediatrics, University of Washington, Seattle, Washington, USA.
Sandra E
0000-0002-6861-6229
SE
Juul
Washington National Primate Research Center, Seattle, Washington, USA.
Richard F
RF
Grant
Washington National Primate Research Center, Seattle, Washington, USA.
LaRene
L
Kuller
Department of Radiology, University of Washington, Seattle, Washington, USA.|Department of Radiology, Seattle Children's Hospital, Seattle, Washington, USA.
Dennis W W
DW
Shaw
Washington National Primate Research Center, Seattle, Washington, USA.
Jason
J
Ogle
Washington National Primate Research Center, Seattle, Washington, USA.
G Michael
GM
Gough
Washington National Primate Research Center, Seattle, Washington, USA.
Wonsok
W
Lee
Washington National Primate Research Center, Seattle, Washington, USA.
Chris
C
English
Department of Neurological Surgery, University of Washington, Seattle, Washington, USA.|Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington, USA.
Robert F
RF
Hevner
Department of Pediatrics, University of Washington, Seattle, Washington, USA.|Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington, USA.
William B
WB
Dobyns
Department of Immunology, University of Washington, Seattle, Washington, USA.|Center for Innate Immunity and Immune Disease, University of Washington, Seattle, Washington, USA.
Michael
M
Gale
Department of Pediatrics, University of Washington, Seattle, Washington, USA.|Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.|Department of Global Health, University of Washington, Seattle, Washington, USA.
Lakshmi
L
Rajagopal
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001921:Brain / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D002794:Choline / Q000378:metabolism; D003401:Creatine / Q000378:metabolism; D004453:Echoencephalography; D005260:Female; D005333:Fetus / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D008254:Macaca nemestrina; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D012367:RNA, Viral / Q000378:metabolism; D016216:Ultrasonography, Prenatal; D000071244:Zika Virus / Q000235:genetics; D000071243:Zika Virus Infection / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology
D012367:RNA, Viral; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; D007294:Inositol; C000179:N-acetylaspartate; D003401:Creatine; D002794:Choline