Human Alveolar Macrophages May Not Be Susceptible to Direct Infection by a Human Influenza Virus.
The current studies were undertaken to determine the susceptibility of human alveolar macrophages (AMs) to influenza A virus (IAV) infection in comparison with autologous peripheral blood-derived monocytes-macrophages (PBMs). AMs and PBMs were exposed to IAV in vitro and examined for their ability to bind and internalize IAV, and synthesize viral proteins and RNA. PBMs but not AMs demonstrated binding and internalization of the virus, synthesizing viral proteins and RNA. Exposure of AMs in the presence of a sialidase inhibitor or anti-IAV antibody resulted in viral protein synthesis by the cells. Exposure of AMs to fluorescein isothiocyanate-labeled IAV in the presence of anti-fluorescein isothiocyanate antibody also resulted in viral protein synthesis. Thus, human AMs are apparently not susceptible to direct infection by a human IAV but are likely to be infected indirectly in the setting of exposure in the presence of antibody that binds the challenging strain of IAV.
Pulmonary and Critical Care Unit, University of Rochester School of Medicine.
David B
DB
Ettensohn
Pulmonary and Critical Care Unit, University of Rochester School of Medicine.
Mark W
MW
Frampton
Division of Infectious Diseases, Department of Internal Medicine, and Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston.
Joan E
JE
Nichols
Division of Infectious Diseases and Immunology, Department of Medicine, New York University School of Medicine, New York.|Division of Infectious Diseases, Department of Internal Medicine, and Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston.
Norbert J
NJ
Roberts
D000328:Adult; D000914:Antibodies, Viral / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D009980:Influenza A virus / Q000276:immunology / Q000502:physiology*; D007251:Influenza, Human / Q000276:immunology / Q000473:pathology*; D016676:Macrophages, Alveolar / Q000276:immunology / Q000821:virology*; D008297:Male; D056189:Viral Tropism; D053585:Virus Attachment; D053586:Virus Internalization; D014779:Virus Replication; D055815:Young Adult
D000914:Antibodies, Viral
human alveolar macrophages; influenza virus; macrophages; monocytes