Multifunctional soft hybrid bio-platforms based on nano-silver and natural compounds.
Novel nanohybrids consisting of nano-Ag, chitosan, lipids and phyto-compounds (chlorophyll a and curcumin) have been achieved through a simple bottom-up strategy, resulting in stable (ZP=-30.9mV) and spherical-shaped nano-entities with size <200nm (estimated by AFM analysis and DLS measurements). The formation of these biohybrids was monitored by absorption and emission spectroscopy, exploiting the spectral fingerprint of chlorophyll a. The bio-performances of these hybrid materials such as: high antioxidant activity (96.63%), strong biocidal properties against Escherichia coli ATCC 8738 (exhibiting an inhibition zone diameter of 32mm), hemocompatibility, in vitro cytotoxicity against HT-29 cancer cells and no toxicity to normal cells (in the biohybrid concentration range of 5.7-17%), make them promising candidates in bio-applications (antimicrobial and antioxidant coating, cancer treatment).
University of Bucharest, Faculty of Physics, Department of Electricity and Magnetism, Solid-State Physics, and Biophysics, 405 Atomistilor Street, Bucharest-Magurele 077125, Romania.
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Barbinta-Patrascu
University "Politehnica" of Bucharest, Faculty of Applied Chemistry and Materials Science, 1-7 Polizu Str., 011061 Bucharest, Romania. Electronic address: nicoleta.badea@gmail.com.
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Badea
University "Politehnica" of Bucharest, Faculty of Applied Chemistry and Materials Science, 1-7 Polizu Str., 011061 Bucharest, Romania.
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Pirvu
Horia Hulubei National Institute for Physics and Nuclear Engineering (IFIN-HH), Department of Life and Environmental Physics, Bucharest-Magurele 077125, Romania.
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Bacalum
University "Politehnica" of Bucharest, Faculty of Applied Chemistry and Materials Science, 1-7 Polizu Str., 011061 Bucharest, Romania.
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Ungureanu
Aghoras Invent SRL (AquaNano), 48 Muntii Carpati Str., Bucharest, Romania.
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Nadejde
University of Bucharest, Faculty of Physics, Department of Electricity and Magnetism, Solid-State Physics, and Biophysics, 405 Atomistilor Street, Bucharest-Magurele 077125, Romania; Aghoras Invent SRL (AquaNano), 48 Muntii Carpati Str., Bucharest, Romania.
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Ion
University "Politehnica" of Bucharest, Faculty of Applied Chemistry and Materials Science, 1-7 Polizu Str., 011061 Bucharest, Romania. Electronic address: ileana_brandusa@yahoo.com.
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Rau
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001688:Biological Products / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D004926:Escherichia coli / Q000187:drug effects; D005492:Folic Acid / Q000737:chemistry; D019073:HT29 Cells; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D010316:Particle Size; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D001688:Biological Products; D002734:Chlorophyll; D012834:Silver; D005492:Folic Acid; D000077194:Chlorophyll A
Chitosan; Chlorophyll a; Curcumin; Nano-silver; Soft biohybrids