In-vitro depth-dependent hyperthermia of human mammary gland adenocarcinoma.
Nanoparticle mediated photothermal ablation of cancerous tissue shows promising results and applicability as a highly efficacious treatment method. As a majority of the photothermal work has been conducted with minimal attenuation of the laser before reaching the nanoparticles within surface seeded tumors in-vivo or through buffered media in-vitro, it is important to understand the effects of greater laser attenuation on photothermal efficacy mediated by changes in the scattering and absorption of the laser. Photothermal efficacy using a near infrared (NIR) 785nm laser irradiating polystyrene (PS) stabilized magnetite (Fe3O4) nanoparticles (PS-Fe3O4) is examined on MDA-MB-231 human mammary gland adenocarcinoma in-vitro. Agarose gel columns of various heights were created to simulate soft tissue and subsequently used for NIR laser attenuation. Polystyrene was found to significantly improve magnetite nanoparticle stability in serum containing media and modified Hank's Balanced Salt Solution and was able to induce significant hyperthermic ablation at mass concentrations which also did not elicit significant innate toxicity. Furthermore it was found that the polystyrene coating significantly reduced innate toxicity over 48h compared to uncoated magnetite. Agar gel layers provided similar optical attenuation in the NIR region to skin and prostate.
The Materials Science and Engineering Program, Dept. of Mechanical and Materials Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USA.
Andrew W
AW
Dunn
The Materials Science and Engineering Program, Dept. of Mechanical and Materials Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USA.
Yu
Y
Zhang
Department of Physics, University of Cincinnati, Cincinnati, OH 45221, USA.
David
D
Mast
The James L. Winkle College of Pharmacy, University of Cincinnati, Cincinnati, OH 45267, USA.
Giovanni M
GM
Pauletti
Nano Biomedical Research Center, School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China.
Hong
H
Xu
Department of Geological & Environmental Sciences, Stanford University, Stanford, CA 94305, USA.
Jiaming
J
Zhang
Department of Geological & Environmental Sciences, Stanford University, Stanford, CA 94305, USA.
Rodney C
RC
Ewing
East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai 200092, China; The Materials Science and Engineering Program, Dept. of Mechanical and Materials Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USA. Electronic address: donglu.shi@uc.edu.
Donglu
D
Shi
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003470:Culture Media; D005260:Female; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D007259:Infrared Rays; D007834:Lasers; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry
D003470:Culture Media; D058185:Magnetite Nanoparticles; D011137:Polystyrenes; D052203:Ferrosoferric Oxide
Hyperthermia; In-vitro; Nanoparticle