Biocompatible lutein-polymer-lipid nanocapsules: Acute and subacute toxicity and bioavailability in mice.
Lutein-poly-(lactic-co-glycolic acid) (PLGA)-phospholipid (PL) nanocapsules were prepared (henceforth referred as lutein nanocapsules) and studied for acute, subacute oral toxicity and bioavailability of lutein in mice. Prior to examining the safety of lutein nanocapsules, particle size, zeta potential, surface morphology and interaction between lutein, PLGA and PL were studied. In acute study, mice were gavaged with a single dose of lutein nanocapsules at 0.1, 1, 10 and 100mg/kg body weight (BW) and examined for 2weeks, while in subacute study, daily mice were gavaged with a dose of 1 and 10mg/kg BW for 4weeks. Results revealed that mean size and zeta value of lutein nanocapsules were 140nm and -44mV, respectively. Acute and subacute toxicity studies did not show any mortality or treatment related adverse effect in clinical observations, ophthalmic examinations, body and organ weights. No toxicity related findings were observed in hematology, histopathology and other blood and tissue clinical chemistry parameters. In subacute study, no observed adverse effect level (NOAEL) of lutein nanocapsules was found to be at a dose of 10mg/kg BW. Feeding lutein nanocapsules resulted in a significant (p<0.01) increase in lutein level in plasma and tissue compared to the control group. Lutein nanocapsules did not cause toxicity in mice. However, human trials are warranted.
Department of Biochemistry, CSIR - Central Food Technological Research Institute, Mysore, Karnataka, India.
Arunkumar
A
Ranganathan
Department of Biochemistry, CSIR - Central Food Technological Research Institute, Mysore, Karnataka, India.
Ravi
R
Hindupur
Department of Biochemistry, CSIR - Central Food Technological Research Institute, Mysore, Karnataka, India. Electronic address: baskaranv@cftri.res.in.
Baskaran
B
Vallikannan
D000284:Administration, Oral; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D001682:Biological Availability; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D008055:Lipids / Q000633:toxicity*; D014975:Lutein / Q000097:blood / Q000633:toxicity* / Q000652:urine; D055442:Metabolome / Q000187:drug effects; D051379:Mice; D018625:Microscopy, Atomic Force; D053769:Nanocapsules / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D011108:Polymers / Q000633:toxicity*; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D014018:Tissue Distribution / Q000187:drug effects; D023382:Toxicity Tests, Acute
D001672:Biocompatible Materials; D008055:Lipids; D053769:Nanocapsules; D011108:Polymers; D014975:Lutein
Acute and subacute treatment; Biocompatible; Carotenoids; Lutein; Nanocapsules; PLGA; Phospholipid; Toxicity