Protective effect of Cornus walteri Wangerin leaf against UVB irradiation induced photoaging in human reconstituted skin.
BACKGROUND
Cornus walteri Wangerin has been used in oriental traditional medicine for the treatment of antidiarrheal and inflammation.


OBJECTIVE
The efficacy of Cornus walteri Wangerin on skin anti-photoaging was investigated.


METHODS
Hydrolyzed Cornus walteri Wangerin leaf was tested for the anti-photoaging effects against ultraviolet B (UVB)-induced matrix metalloproteinase (MMP)-1, pro-inflammatory cytokines using human reconstituted skin (KeraSkin™-FT) and also tested for elastase activity in vitro. The MMP-1 and pro-inflammatory cytokine levels of the extract were evaluated by enzyme-linked immunosorbent assay (ELISA).


RESULTS
The extract of hydrolyzed Cornus walteri Wangerin leaf (CWE) had the elastase inhibitory activity (IC50: 0.457mg/mL). CWE inhibited MMP-1 expression up to 61% in comparison with the control group which was not treated using CWE, but exposed to UVB. CWE also showed an inhibitory effect on releasing pro-inflammatory cytokines (IL-6 and IL-8) in KeraSkin™-FT (30% and 57% inhibition at dose of 50μg/mL, respectively).


CONCLUSIONS
CWE is a promising anti-photoaging agent for the treatment of UVB-induced skin.
R&D Center, Saimdang Cosmetics Co., Ltd., 143, Yangcheongsongdae-gil, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do, South Korea. Electronic address: seung-hee@hanmail.net.
Hyun-Chul
HC
Park
R&D Center, Saimdang Cosmetics Co., Ltd., 143, Yangcheongsongdae-gil, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do, South Korea. Electronic address: jungtk@saimdang.co.kr.
Taek Kyu
TK
Jung
R&D Center, Saimdang Cosmetics Co., Ltd., 143, Yangcheongsongdae-gil, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do, South Korea. Electronic address: kmj75@saimdang.co.kr.
Mi Jin
MJ
Kim
R&D Center, Saimdang Cosmetics Co., Ltd., 143, Yangcheongsongdae-gil, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do, South Korea. Electronic address: ksyoonjh@hanmail.net.
Kyung-Sup
KS
Yoon
D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D029768:Cornus / Q000737:chemistry*; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D006801:Humans; D006868:Hydrolysis; D018836:Inflammation Mediators / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D010936:Plant Extracts; D010196:Pancreatic Elastase; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
Anti-photoaging; Cornus walteri Wangerin; Elastase; Inflammation; Matrix-metalloproteinase; UV-B irradiation