Structural elucidation of polysaccharide containing 3-O-methyl galactose from fruiting bodies of Pleurotus citrinopileatus.
A water-soluble polysaccharide containing 3-O-methyl galactose (PCP60W) was isolated from fruiting bodies of Pleurotus citrinopileatus and purified by anion-exchange and gel column chromatography. This polysaccharide has an average molecular weight of 2.74 × 104Da and its structure was elucidated using monosaccharide composition and methylation analysis combined with one- and two-dimensional (COSY, TOCSY, NOESY, HMQC and HMBC) NMR spectroscopy. PCP60W was shown to be a linear partially 3-O-methylated α-galactopyranan comprised of 6-linked galactose, 6-linked 3-O-methyl galactose and 4-linked glucose in a ratio of 3.0:1.0:0.6. This work provides additional evidence for the view that 3-O-methyl galactose is common to the genus Pleurotus.
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China; Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China.
Pengfei
P
He
Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China; Departments of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA; Departments of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA. Electronic address: zhanganqiang@zjut.edu.cn.
Anqiang
A
Zhang
Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China.
Saijing
S
Zhou
Departments of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA; Departments of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Fuming
F
Zhang
Departments of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA; Departments of Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA; Department of Biological Science, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Robert J
RJ
Linhardt
Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China. Electronic address: sun_pl@zjut.edu.cn.
Peilong
P
Sun
D002240:Carbohydrate Sequence; D005690:Galactose / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
D011134:Polysaccharides; D005690:Galactose
3-O-methylated galactopyranose; Edible mushrooms; Pleurotus citrinopileatus; Polysaccharide; Structure