Molecularly imprinted polymer membranes and thin films for the separation and sensing of biomacromolecules.
This review describes recent advances associated with the development of surface imprinting methods for the synthesis of polymeric membranes and thin films, which possess the capability to selectively and specifically recognize biomacromolecules, such as proteins and single- and double-stranded DNA, employing "epitope" or "whole molecule" approaches. Synthetic procedures to create different molecularly imprinted polymer membranes or thin films are discussed, including grafting/in situ polymerization, drop-, dip-, or spin-coating procedures, electropolymerization as well as micro-contact or stamp lithography imprinting methods. Highly sensitive techniques for surface characterization and analyte detection are described, encompassing luminescence and fluorescence spectroscopy, X-ray photoelectron spectroscopy, FTIR spectroscopy, surface-enhanced Raman spectroscopy, atomic force microscopy, quartz crystal microbalance analysis, cyclic voltammetry, and surface plasmon resonance. These developments are providing new avenues to produce bioelectronic sensors and new ways to explore through advanced separation science procedures complex phenomena associated with the origins of biorecognition in nature.
Australian Centre for Research on Separation Science (ACROSS), Centre for Green Chemistry, Monash University, Melbourne, Australia.
Reinhard I
RI
Boysen
Australian Centre for Research on Separation Science (ACROSS), Centre for Green Chemistry, Monash University, Melbourne, Australia.|School of Agricultural and Wine Sciences, Faculty of Science, Charles Sturt University, Wagga Wagga, Australia.
Lachlan J
LJ
Schwarz
Australian Centre for Research on Separation Science (ACROSS), Centre for Green Chemistry, Monash University, Melbourne, Australia.|Department of Bioengineering, Faculty of Engineering, McGill University, Montreal, Canada.
Dan V
DV
Nicolau
Australian Centre for Research on Separation Science (ACROSS), Centre for Green Chemistry, Monash University, Melbourne, Australia.
Milton T W
MT
Hearn
D015374:Biosensing Techniques; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D046911:Macromolecular Substances / Q000032:analysis* / Q000302:isolation & purification*; D008567:Membranes, Artificial; D011108:Polymers / Q000138:chemical synthesis*
D046911:Macromolecular Substances; D008567:Membranes, Artificial; D011108:Polymers
Biomacromolecules; Membranes; Molecularly imprinted polymers; Surface imprinting; Thin films