Across intra-mammalian stages of the liver f luke Fasciola hepatica: a proteomic study.
Fasciola hepatica is the agent of fasciolosis, a foodborne zoonosis that affects livestock production and human health. Although flukicidal drugs are available, re-infection and expanding resistance to triclabendazole demand new control strategies. Understanding the molecular mechanisms underlying the complex interaction with the mammalian host could provide relevant clues, aiding the search for novel targets in diagnosis and control of fasciolosis. Parasite survival in the mammalian host is mediated by parasite compounds released during infection, known as excretory/secretory (E/S) products. E/S products are thought to protect parasites from host responses, allowing them to survive for a long period in the vertebrate host. This work provides in-depth proteomic analysis of F. hepatica intra-mammalian stages, and represents the largest number of proteins identified to date for this species. Functional classification revealed the presence of proteins involved in different biological processes, many of which represent original findings for this organism and are important for parasite survival within the host. These results could lead to a better comprehension of host-parasite relationships, and contribute to the development of drugs or vaccines against this parasite.
Unidad de Biología Parasitaria, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Montevideo, Uruguay.|Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Lucía Sánchez
LS
Di Maggio
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.|Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Lucas
L
Tirloni
Centro de Pesquisas em Biologia Molecular e Funcional, Instituto Nacional de Ciência e Tecnologia em Tuberculose, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.|Department of Chemical Physiology, The Scripps Research Institute, CA, Unites States of America.
Antonio F M
AF
Pinto
Department of Chemical Physiology, The Scripps Research Institute, CA, Unites States of America.
Jolene K
JK
Diedrich
Department of Chemical Physiology, The Scripps Research Institute, CA, Unites States of America.
John R
JR
Yates Iii
Departamento de Inmunología, Facultad de Veterinaria, Universidad de la República Oriental del Uruguay, Montevideo, Uruguay.
Uruguaysito
U
Benavides
Unidad de Biología Parasitaria, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Montevideo, Uruguay.
Carlos
C
Carmona
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.|Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Itabajara
I
da Silva Vaz
Unidad de Biología Parasitaria, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Montevideo, Uruguay.
Patricia
P
Berasain
D000818:Animals; D005210:Fasciola hepatica / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D015801:Helminth Proteins / Q000378:metabolism*; D006790:Host-Parasite Interactions; D008099:Liver / Q000469:parasitology*; D040901:Proteomics / Q000379:methods*
D015801:Helminth Proteins