Expression of claudins, occludin, junction adhesion molecule A and zona occludens 1 in canine organs.
Tight junctions are the outermost structures of intercellular junctions and are classified as transmembrane proteins. These factors form selective permeability barriers between cells, act as paracellular transporters and regulate structural and functional polarity of cells. Although tight junctions have been previously studied, comparison of the transcriptional‑translational levels of these molecules in canine organs remains to be investigated. In the present study, organ‑specific expression of the tight junction proteins, claudin, occludin, junction adhesion molecule A and zona occludens 1 was examined in the canine duodenum, lung, liver and kidney. Results of immunohistochemistry analysis demonstrated that the tight junctions were localized in intestinal villi and glands of the duodenum, bronchiolar epithelia and alveolar walls of the lung, endometrium and myometrium of the hepatocytes, and the distal tubules and glomeruli of the kidney. These results suggest that tight junctions are differently expressed in organs, and therefore may be involved in organ‑specific functions to maintain physiological homeostasis.
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
Changhwan
C
Ahn
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
Da-Hye
DH
Shin
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
Dongoh
D
Lee
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
Su-Myung
SM
Kang
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
Ju-Hyung
JH
Seok
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
Hee Young
HY
Kang
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
Eui-Bae
EB
Jeung
D000818:Animals; D057167:Claudins / Q000032:analysis* / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D004386:Duodenum / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D015870:Gene Expression; D062745:Junctional Adhesion Molecule A / Q000032:analysis* / Q000235:genetics; D007668:Kidney / Q000378:metabolism / Q000648:ultrastructure; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure; D008168:Lung / Q000378:metabolism / Q000648:ultrastructure; D062793:Occludin / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D062826:Zonula Occludens-1 Protein / Q000032:analysis / Q000235:genetics*
D057167:Claudins; D062745:Junctional Adhesion Molecule A; D062793:Occludin; D012333:RNA, Messenger; D062826:Zonula Occludens-1 Protein