Silencing of cZNF292 circular RNA suppresses human glioma tube formation via the Wnt/β-catenin signaling pathway.
CircRNA is a novel type of RNA molecule formed by a covalently closed loop which have no 5'-3' polarity and possess no polyA tail and relatively stable due to the cyclic structure. Therefore, they may serve as potential targets and diagnosis biomarkers for tumor therapy. cZNF292 is an important circular oncogenic RNA and plays a critical role in the progression of tube formation. This study is aimed at exploring the role of cZNF292 in human glioma tube formation and its potential mechanism of action. We found that cZNF292 silencing suppresses tube formation by inhibiting glioma cell proliferation and cell cycle progression. Cell cycle progression in human glioma U87MG and U251 cells was halted at S/G2/M phase via the Wnt/β-catenin signaling pathway and related genes such as PRR11, Cyclin A, p-CDK2, VEGFR-1/2, p-VEGFR-1/2 and EGFR. The results suggest that cZNF292 silencing plays an important role in the tube formation process and has potential for application as a therapeutic target and biomarker in glioma.
Department of Clinical Laboratory, Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vessels and Neural Degeneration, Tianjin, China.
Ping
P
Yang
Department of Neurology, Tianjin Medical University General Hospital, Tianjin Neurological Institute, Tianjin, China.
Zhijun
Z
Qiu
Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, Key Laboratory of Educational Ministry of China, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
Yuan
Y
Jiang
Department of Pediatrics, Division of Hematology/Oncology, Aflac Cancer and Blood Disorders Center, Emory University School of Medicine, GA, USA.
Lei
L
Dong
School of Pharmacal Sciences, Tianjin Medical University, Tianjin, China.
Wensheng
W
Yang
Department of Genetics, College of Basic Medicine, Tianjin Medical University, Tianjin, China.
Chao
C
Gu
Department of Genetics, College of Basic Medicine, Tianjin Medical University, Tianjin, China.
Guang
G
Li
Department of Clinical Laboratory, Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vessels and Neural Degeneration, Tianjin, China.
Yu
Y
Zhu
D017209:Apoptosis; D002352:Carrier Proteins; D002453:Cell Cycle; D015687:Cell Hypoxia; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000098:blood supply / Q000235:genetics* / Q000473:pathology; D006801:Humans; D009389:Neovascularization, Pathologic; D009419:Nerve Tissue Proteins; D012313:RNA / Q000037:antagonists & inhibitors* / Q000235:genetics; D000079962:RNA, Circular; D014407:Tumor Cells, Cultured; D060449:Wnt Signaling Pathway / Q000235:genetics*
D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; D000079962:RNA, Circular; C570380:ZNF292 protein, human; D012313:RNA
cZNF292; circular RNAs; glioma; proliferation; tube formation