LPS-Induced Macrophage Activation and Plasma Membrane Fluidity Changes are Inhibited Under Oxidative Stress.
Macrophage activation is essential for a correct and efficient response of innate immunity. During oxidative stress membrane receptors and/or membrane lipid dynamics can be altered, leading to dysfunctional cell responses. Our aim is to analyze membrane fluidity modifications and cell function under oxidative stress in LPS-activated macrophages. Membrane fluidity of individual living THP-1 macrophages was evaluated by the technique two-photon microscopy. LPS-activated macrophage function was determined by TNFα secretion. It was shown that LPS activation causes fluidification of macrophage plasma membrane and production of TNFα. However, oxidative stress induces rigidification of macrophage plasma membrane and inhibition of cell activation, which is evidenced by a decrease of TNFα secretion. Thus, under oxidative conditions macrophage proinflammatory response might develop in an inefficient manner.
Unitat de Biofísica, Departament de Bioquímica i de Biologia Molecular and Centre d'Estudis en Biofísica (CEB), Facultat de Medicina, Universitat Autònoma de Barcelona (UAB), 08193, Bellaterra, Barcelona, Spain.|Unitat d'Immunologia, Institut de Biotecnologia i de Biomedicina (IBB), Universitat Autònoma de Barcelona (UAB), 08193, Bellaterra, Barcelona, Spain.
Carlos
C
de la Haba
Unitat de Biofísica, Departament de Bioquímica i de Biologia Molecular and Centre d'Estudis en Biofísica (CEB), Facultat de Medicina, Universitat Autònoma de Barcelona (UAB), 08193, Bellaterra, Barcelona, Spain.
Antoni
A
Morros
Unitat d'Immunologia, Institut de Biotecnologia i de Biomedicina (IBB), Universitat Autònoma de Barcelona (UAB), 08193, Bellaterra, Barcelona, Spain.
Paz
P
Martínez
Unitat d'Immunologia, Institut de Biotecnologia i de Biomedicina (IBB), Universitat Autònoma de Barcelona (UAB), 08193, Bellaterra, Barcelona, Spain. joseramon.palacio@uab.es.
José R
JR
Palacio
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000276:immunology*; D008262:Macrophage Activation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism*; D008560:Membrane Fluidity / Q000276:immunology*; D021962:Membrane Microdomains / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress
D008070:Lipopolysaccharides; D006861:Hydrogen Peroxide
Laurdan; Lipid rafts; Macrophages; Membrane fluidity; Oxidative stress; TLR4