Nanomolar detection of methylparaben by a cost-effective hemoglobin-based biosensor.
This work describes the development of a new biosensor for methylparaben determination using electrocatalytic properties of hemoglobin in the presence of hydrogen peroxide. The voltammetric oxidation of methylparaben by the proposed biosensor in phosphate buffer (pH=7.0), a physiological pH, was studied and it was confirmed that methylparaben undergoes a one electron-one proton reaction in a diffusion-controlled process. The biosensor was fabricated by carbon paste electrode modified with hemoglobin and multiwalled carbon nanotube. Based on the excellent electrochemical properties of the modified electrode, a sensitive voltammetric method was used for determination of methylparaben within a linear range from 0.1 to 13μmolL(-1) and detection limit of 25nmolL(-1). The developed biosensor possessed accurate and rapid response to methylparaben and showed good sensitivity, stability, and repeatability. Finally, the applicability of the proposed biosensor was verified by methylparaben evaluation in various real samples.
Freiburg Materials Research Center, FMF, University of Freiburg, Stefan-Meier-Str.21, 79104 Freiburg, Germany; Laboratory for Sensors, Department of Microsystems Engineering, IMTEK, University of Freiburg, 79110 Freiburg, Germany. Electronic address: ali.hajian@fmf.uni-freiburg.de.
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Hajian
Department of Physical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, 65174, Hamedan, Iran.
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Ghodsi
Department of Physical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, 65174, Hamedan, Iran.
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Afraz
Freiburg Materials Research Center, FMF, University of Freiburg, Stefan-Meier-Str.21, 79104 Freiburg, Germany.
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Yurchenko
Freiburg Materials Research Center, FMF, University of Freiburg, Stefan-Meier-Str.21, 79104 Freiburg, Germany; Laboratory for Sensors, Department of Microsystems Engineering, IMTEK, University of Freiburg, 79110 Freiburg, Germany.
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Urban
D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D055664:Electrochemical Techniques; D004566:Electrodes; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry; D010084:Oxidation-Reduction; D010226:Parabens / Q000032:analysis*; D013499:Surface Properties
D006454:Hemoglobins; D037742:Nanotubes, Carbon; D010226:Parabens; C015358:methylparaben; D006861:Hydrogen Peroxide
Biosensor; Hemoglobin; Methylparaben; Modified carbon paste electrode, voltammetry