The gene therapy of collagen-induced arthritis in rats by intramuscular administration of the plasmid encoding TNF-binding domain of variola virus CrmB protein.
Wistar rats with collagen-induced arthritis were intramuscularly injected with the recombinant plasmid pcDNA/sTNF-BD encoding the sequence of the TNF-binding protein domain of variola virus CrmB protein (VARV sTNF-BD) or the pcDNA3.1 vector. Quantitative analysis showed that the histopathological changes in the hind-limb joints of rats were most severe in the animals injected with pcDNA3.1 and much less severe in the group of rats injected with pcDNA/sTNF-BD, which indicates that gene therapy of rheumatoid arthritis is promising in the case of local administration of plasmids governing the synthesis of VARV immunomodulatory proteins.
Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk oblast, 633159, Russia. snshchel@vector.nsc.ru.
S N
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Shchelkunov
Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk oblast, 633159, Russia.
O S
OS
Taranov
Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk oblast, 633159, Russia.
T V
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Tregubchak
Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk oblast, 633159, Russia.
R A
RA
Maksyutov
Research Institute of Clinical Immunology, Siberian Branch, Russian Academy of Medical Sciences, ul. Yadrintsevskaya 14, Novosivirsk, 630099, Russia.
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Silkov
Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk oblast, 633159, Russia.
A E
AE
Nesterov
Research Institute of Clinical Immunology, Siberian Branch, Russian Academy of Medical Sciences, ul. Yadrintsevskaya 14, Novosivirsk, 630099, Russia.
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SV
Sennikov
D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D001172:Arthritis, Rheumatoid / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D002352:Carrier Proteins / Q000008:administration & dosage* / Q000235:genetics*; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006614:Hindlimb / Q000473:pathology; D007273:Injections, Intramuscular; D008297:Male; D017208:Rats, Wistar; D013585:Synovitis / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D016896:Treatment Outcome; D012901:Variola virus; D014764:Viral Proteins / Q000008:administration & dosage* / Q000235:genetics*
D002352:Carrier Proteins; D014764:Viral Proteins