A pre-classification strategy based on UPLC-Triple-TOF/MS for metabolic screening and identification of Radix glehniae in rats.
Traditional Chinese Medicines (TCMs) have gained increasing popularity in modern society. However, the profiles of TCMs in vivo are still unclear owing to their complexity and low level in vivo. In this study, UPLC-Triple-TOF techniques were employed for data acquiring, and a novel pre-classification strategy was developed to rapidly and systematically screen and identify the absorbed constituents and metabolites of TCMs in vivo using Radix glehniae as the research object. In this strategy, pre-classification for absorbed constituents was first performed according to the similarity of their structures. Then representative constituents were elected from every class and analyzed separately to screen non-target absorbed constituents and metabolites in biosamples. This pre-classification strategy is basing on target (known) constituents to screen non-target (unknown) constituents from the massive data acquired by mass spectrometry. Finally, the screened candidate compounds were interpreted and identified based on a predicted metabolic pathway, well - studied fragmentation rules, a predicted metabolic pathway, polarity and retention time of the compounds, and some related literature. With this method, a total of 111 absorbed constituents and metabolites of Radix glehniae in rats' urine, plasma, and bile samples were screened and identified or tentatively characterized successfully. This strategy provides an idea for the screening and identification of the metabolites of other TCMs.
School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei, 050017, China.
Shuang
S
Wang
School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei, 050017, China.
Pengcheng
P
Qi
School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei, 050017, China.
Na
N
Zhou
School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei, 050017, China.
Minmin
M
Zhao
School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei, 050017, China.
Weijing
W
Ding
Shijiazhuang Yiling Pharmaceutical Co. Ltd, Shijiazhuang, Hebei, 050035, China.
Song
S
Li
Shijiazhuang Yiling Pharmaceutical Co. Ltd, Shijiazhuang, Hebei, 050035, China.
Minyan
M
Liu
School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei, 050017, China. qiaowang88@hotmail.com.|Institute of Chinese Integrative Medicine, Hebei Medical University, Shijiazhuang, Hebei, 050017, China. qiaowang88@hotmail.com.
Qiao
Q
Wang
Department of Pharmacy, the First Hospital of Shijiazhuang, Shijiazhuang, Hebei, 050011, China. jshm2008@sina.com.
Shumin
S
Jin
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000378:metabolism*; D011564:Furocoumarins / Q000032:analysis / Q000378:metabolism; D008297:Male; D053858:Metabolic Networks and Pathways; D051381:Rats; D017207:Rats, Sprague-Dawley; D012603:Scopoletin / Q000032:analysis / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*
D003374:Coumarins; D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; C031534:imperatorin; D012603:Scopoletin
Coumarin; Metabolic profile; Radix glehniae; UPLC-Triple-TOF/MS