Plasma membrane order and fluidity are diversely triggered by elicitors of plant defence.
Although plants are exposed to a great number of pathogens, they usually defend themselves by triggering mechanisms able to limit disease development. Alongside signalling events common to most such incompatible interactions, modifications of plasma membrane (PM) physical properties could be new players in the cell transduction cascade. Different pairs of elicitors (cryptogein, oligogalacturonides, and flagellin) and plant cells (tobacco and Arabidopsis) were used to address the issue of possible modifications of plant PM biophysical properties induced by elicitors and their links to other events of the defence signalling cascade. We observed an increase of PM order whatever the elicitor/plant cell pair used, provided that a signalling cascade was induced. Such membrane modification is dependent on the NADPH oxidase-mediated reactive oxygen species production. Moreover, cryptogein, which is the sole elicitor able to trap sterols, is also the only one able to trigger an increase in PM fluidity. The use of cryptogein variants with altered sterol-binding properties confirms the strong correlation between sterol removal from the PM and PM fluidity enhancement. These results propose PM dynamics as a player in early signalling processes triggered by elicitors of plant defence.
Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137 Brno, Czech Republic.
Roman
R
Sandor
Agroécologie, AgroSup Dijon, CNRS, INRA, Université Bourgogne Franche-Comté, F-21000 Dijon, France.
Christophe
C
Der
Agroécologie, AgroSup Dijon, CNRS, INRA, Université Bourgogne Franche-Comté, F-21000 Dijon, France.
Kevin
K
Grosjean
Agroécologie, AgroSup Dijon, CNRS, INRA, Université Bourgogne Franche-Comté, F-21000 Dijon, France.
Iulia
I
Anca
Agroécologie, AgroSup Dijon, CNRS, INRA, Université Bourgogne Franche-Comté, F-21000 Dijon, France.
Elodie
E
Noirot
Agroécologie, AgroSup Dijon, CNRS, INRA, Université Bourgogne Franche-Comté, F-21000 Dijon, France.
Nathalie
N
Leborgne-Castel
Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137 Brno, Czech Republic.
Jan
J
Lochman
Agroécologie, AgroSup Dijon, CNRS, INRA, Université Bourgogne Franche-Comté, F-21000 Dijon, France.
Françoise
F
Simon-Plas
Agroécologie, AgroSup Dijon, CNRS, INRA, Université Bourgogne Franche-Comté, F-21000 Dijon, France pgerbeau@u-bourgogne.fr.
Patricia
P
Gerbeau-Pissot
D017360:Arabidopsis / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology*; D060467:Disease Resistance / Q000502:physiology*; D008560:Membrane Fluidity / Q000502:physiology*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010935:Plant Diseases; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013050:Spectrometry, Fluorescence; D014026:Nicotiana / Q000502:physiology
D017382:Reactive Oxygen Species
Cryptogein mutants; elicitors; fluidity; membrane order; plant defence; plasma membrane; reactive oxygen species; signalling.