[Activation of peripheral T lymphocytes in children with epilepsy and production of cytokines].
Objective To study the state of peripheral T lymphocytes and cytokine levels in children with epilepsy. Methods Twenty children with epilepsy and 20 healthy age-matched children were recruited and their peripheral blood was collected. The activation of T lymphocytes was evaluated by detecting the expressions of CD25, CD69 and cytotoxic T lymphocyte-assicated antigen 4 (CTLA4). The function of T lymphocytes was evaluated by detecting the expressions of interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), IL-17A and IL-6. The activation of regulatory T cells (Tregs) was evaluated by detecting the expression of IL-10. Results Children with epilepsy had higher expressions of CD25, CD69 and CTLA-4 in T lymphocytes than the controls did. The expressions of IFN-γ, TNF-α, IL-17A and IL-6 in T lymphocytes of children with epilepsy were higher than those of the controls. Frequency of Tregs producing IL-10 was higher in children with epilepsy as compared with the controls. Conclusion Peripheral T lymphocytes of children with epilepsy are activated and produce cytokines.
Department of Pediatrics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Jie
J
Yang
Department of Pediatrics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Chongkang
C
Hu
Department of Pediatrics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. *Corresponding author, E-mail: jiangx@fmmu.edu.cn.
Xun
X
Jiang
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D060908:CTLA-4 Antigen / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000276:immunology*; D004827:Epilepsy / Q000276:immunology*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology; D020381:Interleukin-17 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male
D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D016207:Cytokines; C508609:IL10 protein, human; D020381:Interleukin-17; D015850:Interleukin-6; D016753:Interleukin-10