Accelerated cell-surface interlocking on plasma polymer-modified porous ceramics.
Excellent osseointegration of permanent implants is crucial for the long lasting success of the implantation. To improve the osseointegrative potential, bio-inert titanium alloy surfaces (Ti6Al4V) are modified by plasma chemical oxidation (PCO®) of the titanium-oxide layer to a non-stoichiometric, amorphous calcium phosphate layer. The native titanium-oxide film measuring only a few nanometers is converted by PCO® to a thick porous calcium phosphate layer of about 10μm. In a second step the PCO surface is combined with a cell adhesive plasma-polymerized allylamine (PPAAm) nano film (5 and 50nm). Independent of the PPAAm coating homogeneity, the human osteoblast-like MG-63 cells show a remarkable increase in cell size and well-developed filopodia. Analyses of the actin cytoskeleton reveal that the cells mold to the pore shape of the PPAAm-covered PCO, thereby establishing a strong attachment to the surface. Interestingly, we could demonstrate that even though our untreated PCO shows excellent hydrophilicity, this alone is not sufficient to facilitate fast cell spreading, but the positive surface charges mediated by PPAAm. This multilayer composite material guarantees enhanced interlocking of the cells with the porous surface.
Dept. of Cell Biology, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, Germany. Electronic address: henrike.rebl@med.uni-rostock.de.
Henrike
H
Rebl
Leibniz-Institute for Plasma Science and Technology e.V. (INP), Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany. Electronic address: finke@inp-greifswald.de.
Birgit
B
Finke
Innovent e.V. Technology Development, Prüssingstr. 27 B, 07745 Jena, Germany. Electronic address: js@innovent-jena.de.
Jürgen
J
Schmidt
Institute of Physics, University of Greifswald, Felix-Hausdorff-Str. 6, 17489 Greifswald, Germany. Electronic address: hm120633@uni-greifswald.de.
Heba S
HS
Mohamad
Leibniz-Institute for Plasma Science and Technology e.V. (INP), Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany. Electronic address: ihrke@inp-greifswald.de.
Roland
R
Ihrke
Institute of Physics, University of Greifswald, Felix-Hausdorff-Str. 6, 17489 Greifswald, Germany. Electronic address: helm@uni-greifswald.de.
Christiane A
CA
Helm
Dept. of Cell Biology, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, Germany. Electronic address: barbara.nebe@med.uni-rostock.de.
J Barbara
JB
Nebe
D008841:Actin Cytoskeleton / Q000187:drug effects; D000497:Alloys; D000499:Allylamine / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002516:Ceramics / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
D000497:Alloys; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D011108:Polymers; C519480:amorphous calcium phosphate; C031462:titanium alloy (TiAl6V4); D000499:Allylamine; D014025:Titanium
Actin cytoskeleton; Cell morphology; Ceramic; Osteoblast; Plasma chemical oxidation; Plasma polymer film; Surface charge; Titanium implant; Water contact angle