Development of PLGA-coated β-TCP scaffolds containing VEGF for bone tissue engineering.
Bone tissue engineering is sought to apply strategies for bone defects healing without limitations and short-comings of using either bone autografts or allografts and xenografts. The aim of this study was to fabricate a thin layer poly(lactic-co-glycolic) acid (PLGA) coated beta-tricalcium phosphate (β-TCP) scaffold with sustained release of vascular endothelial growth factor (VEGF). PLGA coating increased compressive strength of the β-TCP scaffolds significantly. For in vitro evaluations, canine mesenchymal stem cells (cMSCs) and canine endothelial progenitor cells (cEPCs) were isolated and characterized. Cell proliferation and attachment were demonstrated and the rate of cells proliferation on the VEGF released scaffold was significantly more than compared to the scaffolds with no VEGF loading. A significant increase in expression of COL1 and RUNX2 was indicated in the scaffolds loaded with VEGF and MSCs compared to the other groups. Consequently, PLGA coated β-TCP scaffold with sustained and localized release of VEGF showed favourable results for bone regeneration in vitro, and this scaffold has the potential to use as a drug delivery device in the future.
Department of Oral and Maxillofacial Surgery, Dental Research Center, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Craniomaxillofacial Surgery, School of Medicine, University of Antwerp, Antwerp, Belgiumo.
Arash
A
Khojasteh
Marquette University School of Dentistry, Milwaukee, WI 53233, USA; Dental Biomaterials Department, School of Dentistry, Tehran University of Medical Scien﻿ces, Tehran, Iran. Electronic address: farahnaz.fahimipour@marquette.edu.
Farahnaz
F
Fahimipour
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biologyand Technology, ACECR, Tehran, Iran. Electronic address: eslami@royaninstitute.org.
Mohamadreza Baghaban
MB
Eslaminejad
Department of Oral and Maxillofacial Surgery, Dental Research Center, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohammad
M
Jafarian
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biologyand Technology, ACECR, Tehran, Iran.
Shahrbanoo
S
Jahangir
Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Farshid
F
Bastami
Marquette University School of Dentistry, Milwaukee, WI 53233, USA; Dental Biomaterials Department, School of Dentistry, Tehran University of Medical Scien﻿ces, Tehran, Iran; Biomaterials Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
Mohammadreza
M
Tahriri
Biomaterials Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
Akbar
A
Karkhaneh
Marquette University School of Dentistry, Milwaukee, WI 53233, USA; Biomaterials Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran; Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK.
Lobat
L
Tayebi
D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D019245:Compressive Strength; D004285:Dogs; D065546:Drug Liberation; D066026:Endothelial Progenitor Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D060888:Real-Time Polymerase Chain Reaction; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*; D014961:X-Ray Diffraction
D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D042461:Vascular Endothelial Growth Factor A; C485817:beta-tricalcium phosphate; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
PLGA; Scaffold; Tissue engineering; VEGF; β-TCP