Dedifferentiation Effects of Rabbit Regenerating Tissue on Partially Differentiated Cells.
Cell Stemness can be achieved by various reprogramming techniques namely, somatic cell nuclear transfer, cell fusion, cell extracts, and introduction of transcription factors from which induced pluripotent stem cells (iPSCs) are obtained. iPSCs are valuable cell sources for drug screening and human disease modeling. Alternatives to virus-based introduction of transcription factors include application of DNA-free methods and introduction of chemically defined culturing conditions. However, the possibility of tumor development is still a hurdle. By taking advantage of NTERA-2 cells, a human embryonal carcinoma cell line, we obtained partially differentiated cells and examined the dedifferentiation capacity of regenerative tissue from rabbit ears. Results indicated that treatment of partially differentiated NTERA-2 cells with the regenerating tissue-conditioned medium (CM) induced expression of key pluripotency markers as examined by real-time polymerase chain reaction, flow cytometry, and immunocytochemistry techniques. In this study, it is reported for the first time that the CM obtained from rabbit regenerating tissue contains dedifferentiation factors, taking cells back to the pluripotency. This system could be a simple and efficient way to reprogram the differentiated cells and generate iPSCs for clinical applications as this system is not accompanied by any viral vector, and reprograms the cells within 10 days of treatment. The results may convince the genomic experts to study the unknown signaling pathways involved in the dedifferentiation by regenerating tissue-CM to authenticate the reprogramming model.
1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad , Mashhad, Iran .|2 Stem Cell and Regenerative Research Group, Iranian Academic Center for Education , Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran .
Mahdi
M
Mirahmadi
1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad , Mashhad, Iran .|3 Institute of Biochemistry and Molecular Biology II, Heinrich-Heine University , Düsseldorf, Germany .
Saeideh
S
Nakhaei-Rad
1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad , Mashhad, Iran .|2 Stem Cell and Regenerative Research Group, Iranian Academic Center for Education , Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran .|4 Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad , Mashhad, Iran .
Maryam M
MM
Matin
1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad , Mashhad, Iran .
Mina
M
Shahriyari
1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad , Mashhad, Iran .
Morvarid
M
Saeinasab
1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad , Mashhad, Iran .|4 Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad , Mashhad, Iran .
Zahra
Z
Mahmoudi
1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad , Mashhad, Iran .|4 Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad , Mashhad, Iran .
Azadeh
A
Haghighitalab
1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad , Mashhad, Iran .
Naser
N
Mahdavi-Shahri
1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad , Mashhad, Iran .|4 Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad , Mashhad, Iran .
Ahmad Reza
AR
Bahrami
D000818:Animals; D054337:Cell Dedifferentiation; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D008297:Male; D053652:Nuclear Transfer Techniques; D011817:Rabbits; D012038:Regeneration / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism
D014157:Transcription Factors