Raman-based detection of hydroxyethyl starch in kidney allograft biopsies as a potential marker of allograft quality in kidney transplant recipients.
In brain-dead donor resuscitation, hydroxyethyl starch (HES) use has been associated with presence of osmotic-nephrosis-like lesions in kidney transplant recipients. Our aim was to determine whether the presence of HES in protocol renal graft biopsies at three months (M3) after transplantation is associated with renal graft quality. According to the HES administered to the donor during the procurement procedure, two groups of patients were defined according graft exposition to HES: HES group, (N = 20) and control group (N = 6). Detection and relative quantification of HES was performed by Raman spectroscopy microimaging on M3 protocol renal graft biopsies. Statistical analyses were used to investigate the association between Raman data and graft characteristics. HES spectral signal was revealed negative in the control group, whereas it was positive in 40% of biopsies from the HES group. In the HES group, a stronger HES signal was associated with a lower risk of graft failure measured by the Kidney Donor Risk Index (KDRI) and was correlated with the allograft kidney function. Thus, HES accumulation in donor kidney, as probed by Raman biophotonic technique, is correlated with the quality of donor kidney and consequently the graft renal function and graft survival.
UMR CNRS 7369 MEDyC, Université de Reims Champagne-Ardennes, Reims, France.|Nephrology division, Maison Blanche University Hospital, Reims, France.|Biopathology Laboratory, Maison Blanche University Hospital, Reims, France.
Vincent
V
Vuiblet
UMR CNRS 7369 MEDyC, Université de Reims Champagne-Ardennes, Reims, France.
Michael
M
Fere
Department of Anesthesia, Maison Blanche University Hospital, Reims, France.
Ezechiel
E
Bankole
Nephrology division, Maison Blanche University Hospital, Reims, France.
Alain
A
Wynckel
UMR CNRS 7369 MEDyC, Université de Reims Champagne-Ardennes, Reims, France.
Cyril
C
Gobinet
Biopathology Laboratory, Maison Blanche University Hospital, Reims, France.
Philippe
P
Birembaut
UMR CNRS 7369 MEDyC, Université de Reims Champagne-Ardennes, Reims, France.|PICT (Cellular and Tissular Imaging Platform), Université de Reims Champagne- Ardenne, Reims, France.
Olivier
O
Piot
UMR CNRS 7369 MEDyC, Université de Reims Champagne-Ardennes, Reims, France.|Nephrology division, Maison Blanche University Hospital, Reims, France.
Philippe
P
Rieu
D000328:Adult; D000368:Aged; D001926:Brain Death; D005260:Female; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007668:Kidney / Q000378:metabolism*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D012151:Resuscitation; D013059:Spectrum Analysis, Raman; D019737:Transplants / Q000378:metabolism*
D006895:Hydroxyethyl Starch Derivatives