Actinomyces succiniciruminis sp. nov. and Actinomyces glycerinitolerans sp. nov., two novel organic acid-producing bacteria isolated from rumen.
Two bacterial strains, Am4 and G10 were isolated from rumen fluid of different ruminants: cow (Holstein-Friesian) and sheep (Slovenskè merino), respectively. They were isolated from different hosts and regions, but showed 99.2% similarity of the 16S rRNA genes. Both strains are versatile and ferment various sugars to mainly succinate and lactate and small amounts of acetate and formate. The 16S rRNA sequences of Am4 and G10 revealed that they belonged to the genus Actinomyces, and are related to Actinomyces ruminicola JCM 13352Twith 97.0% and 97.4% similarity, respectively. DDH showed strain Am4 and G10 had only 55.8 and 43.3% similarity with the Actinomyces ruminicola JCM 13352T, and had 69.9% similarity among each other. Comparing strain Am4 and G10, gANI value and dDDH were 92.9% and 68.6%, respectively. Additionally, AAI between the strains was 95.8%. MLSA of housekeeping genes showed difference of metG and pheS. The G+C% contents of strain Am4 and G10 were 69.8% and 68.5%, respectively. MK-10(H4) was the principal quinone for strain Am4 (82%) and G10 (91%) with small amounts of MK-10(H8) and MK-10(H6) for both strains. Only MK-9(H4) was detected in strain Am4. MALDI-TOF analysis of protein profiles also revealed that Am4 and G10 are different from each other and from Actinomyces ruminicola JCM 13352T. Based on phylogenetic and physiological characteristics, together with genome comparison and MLSA we propose two novel species in the genus Actinomyces: Actinomyces succiniciruminis sp. nov. (type strain Am4T=TISTR 2317T=DSM 10376T) and Actinomyces glycerinitolerans sp. nov. (type strain G10T=TISTR 2318T=DSM 10377T).
Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands; Thailand Institute of Scientific and Technological Research, 35 Mu 3, Khlong Ha, Khlong Luang, Pathum Thani 12120, Thailand. Electronic address: susakul.palakawongnaayudthaya@wur.nl.
Susakul
S
Palakawong N A
Institute of Biology and Ecology, Pavol Jozef Šafárik University, Šrobárova 2, SK-04180 Košice, Slovak Republic; Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, SK-04001 Košice, Slovak Republic. Electronic address: pristas@saske.sk.
Peter
P
Pristaš
Institute of Biology and Ecology, Pavol Jozef Šafárik University, Šrobárova 2, SK-04180 Košice, Slovak Republic. Electronic address: ludmila.hrehova@student.upjs.sk.
Ludmila
L
Hrehová
Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, SK-04001 Košice, Slovak Republic. Electronic address: javorsky@saske.sk.
Peter
P
Javorský
Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands. Electronic address: fons.stams@wur.nl.
Alfons J M
AJ
Stams
Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands. Electronic address: caroline.plugge@wur.nl.
Caroline M
CM
Plugge
D000190:Actinomyces / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D002417:Cattle / Q000382:microbiology*; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D020043:Genes, Essential / Q000235:genetics; D005990:Glycerol / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012417:Rumen / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary*; D019802:Succinic Acid / Q000378:metabolism
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D019802:Succinic Acid; D005990:Glycerol
Actinomyces; Glycerol tolerance; Organic acid production; Rumen bacteria; Succinate production