Probing the luminal microenvironment of reconstituted epithelial microtissues.
Polymeric microparticles can serve as carriers or sensors to instruct or characterize tissue biology. However, incorporating microparticles into tissues for in vitro assays remains a challenge. We exploit three-dimensional cell-patterning technologies and directed epithelial self-organization to deliver microparticles to the lumen of reconstituted human intestinal microtissues. We also develop a novel pH-sensitive microsensor that can measure the luminal pH of reconstituted epithelial microtissues. These studies offer a novel approach for investigating luminal microenvironments and drug-delivery across epithelial barriers.
UC Berkeley - UCSF Graduate Program in Bioengineering, Berkeley - Berkeley (CA), USA.|Department of Bioengineering and Therapeutic Sciences, UCSF, San Francisco (CA), USA.|Department of Pharmaceutical Chemistry, UCSF, San Francisco (CA), USA.
Alec E
AE
Cerchiari
UC Berkeley - UCSF Graduate Program in Bioengineering, Berkeley - Berkeley (CA), USA.|Department of Bioengineering and Therapeutic Sciences, UCSF, San Francisco (CA), USA.
Karen E
KE
Samy
Department of Pharmaceutical Chemistry, UCSF, San Francisco (CA), USA.
Michael E
ME
Todhunter
UC Berkeley - UCSF Graduate Program in Bioengineering, Berkeley - Berkeley (CA), USA.|Department of Bioengineering and Therapeutic Sciences, UCSF, San Francisco (CA), USA.
Erica
E
Schlesinger
Prolynx LLC, San Francisco (CA), USA.
Jeff
J
Henise
Carl Zeiss Microscopy, LLC, Thornwood (NY), USA.
Christopher
C
Rieken
UC Berkeley - UCSF Graduate Program in Bioengineering, Berkeley - Berkeley (CA), USA.|Department of Pharmaceutical Chemistry, UCSF, San Francisco (CA), USA.
Zev J
ZJ
Gartner
UC Berkeley - UCSF Graduate Program in Bioengineering, Berkeley - Berkeley (CA), USA.|Department of Bioengineering and Therapeutic Sciences, UCSF, San Francisco (CA), USA.
Tejal A
TA
Desai
D018938:Caco-2 Cells; D018929:Cell Culture Techniques / Q000379:methods*; D060833:Cellular Microenvironment; D016503:Drug Delivery Systems; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
D011108:Polymers