Quantitative high-throughput population dynamics in continuous-culture by automated microscopy.
We present a high-throughput method to measure abundance dynamics in microbial communities sustained in continuous-culture. Our method uses custom epi-fluorescence microscopes to automatically image single cells drawn from a continuously-cultured population while precisely controlling culture conditions. For clonal populations of Escherichia coli our instrument reveals history-dependent resilience and growth rate dependent aggregation.
Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.|Department of Physics, University of Illinois at Urbana-Champign, Urbana, IL 61801, USA.
Jason
J
Merritt
Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.|Department of Physics, University of Illinois at Urbana-Champign, Urbana, IL 61801, USA.|Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Seppe
S
Kuehn
D061249:Batch Cell Culture Techniques; D004926:Escherichia coli / Q000166:cytology / Q000254:growth & development*; D008856:Microscopy, Fluorescence