Evaluation of Pre-Transplant Panel Reactive Antibody Levels and Sensitization: A Single-Center Study.
BACKGROUND Sensitization is one of the most important barriers against transplantation. Our aim was to evaluate the sensitization status of our patients awaiting cadaveric transplantation and to identify factors causing sensitization. MATERIAL AND METHODS A total of 140 patients on the cadaveric waiting list during January 2014 were included in this retrospective cross-sectional study. The parametric t-test and the non-parametric chi-square test were used to detect differences between PRA-positive and -negative patients. Multivariate analysis was used to identify factors associated with PRA positivity. One-way analysis of variance was used to compare PRA-negative and -positive results. RESULTS Anti-HCV positivity (p=0.040), history of transfusion (p=0.041), and mean number of blood product transfused (p=0.047) were significantly related to class 1 PRA positivity. History of transfusion (p=0.038) and mean number of blood product transfused (p=0.044) were related to class 2 PRA positivity. The multivariate analysis indicated that transfusion and more than 5 units of blood product transfused were related to either class 1 or class 2 PRA positivity. No associations were found between PRA positivity and pregnancy, transplantation, age, sex, infection, abortion, cardiovascular disease, diabetes mellitus, hepatitis B, or time spent on dialysis and being on the transplantation waiting list. CONCLUSIONS Anti-HCV positivity and transfusion are risk factors for sensitization. Particular emphasis should be given to sensitization and its prevention to reduce waiting time for transplantation.
Department of Nephrology, aydarpaşa Numune Training and Research Hospital, İstanbul, Turkey.
Özgür
Ö
Can
Department of Urology and Transplantation, Haydarpaşa Numune Training and Research Hospital, İstanbul, Turkey.
Ali Murat
AM
Gökçe
Department of Nephrology, Haydarpaşa Numune Training and Research Hospital, İstanbul, Turkey.
Mustafa
M
Canbakan
Department of Medical Genetics, Marmara University Faculty of Medicine, Pendik, İstanbul, Turkey.
Pınar
P
Ata
Department of Nephrology, Haydarpaşa Numune Training and Research Hospital, İstanbul, Turkey.
Gülizar Manga
GM
Şahin
Department of General Surgery, Namık Kemal University School of Medicine, Tekirdağ, Turkey.
Mesut İzzet
Mİ
Titiz
Department of Nephrology, Bakırköy Dr. Sadi Konuk Training and Research Hospital, İstanbul, Turkey.
Süheyla
S
Apaydın
D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D006680:HLA Antigens / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007518:Isoantibodies / Q000097:blood*; D016030:Kidney Transplantation / Q000009:adverse effects; D008297:Male; D008875:Middle Aged; D011247:Pregnancy; D057234:Preoperative Period; D012189:Retrospective Studies; D012307:Risk Factors; D065227:Transfusion Reaction; D014850:Waiting Lists
D006680:HLA Antigens; D007074:Immunoglobulin G; D007518:Isoantibodies