Impact of in Vitro Gastrointestinal Digestion and Transepithelial Transport on Antioxidant and ACE-Inhibitory Activities of Brewer's Spent Yeast Autolysate.
Brewer's spent yeast (BSY) autolysates may have potential applications as food ingredients or nutraceuticals due to their antioxidant and ACE-inhibitory activities. The impact of simulated gastrointestinal (GI) digestion, the interaction with intracellular sources of oxidative stress, the intestinal cell permeability of BSY peptides, and the antioxidant and ACE-inhibitory activities of BSY permeates were assayed. Gastrointestinal digestion of BSY autolysates enhanced antioxidant and ACE-inhibitory activities as measured in vitro. No cytotoxic effects were observed on Caco-2 cells after exposure to the digested BSY autolysates within a concentration range of 0.5 to 3.0 mg of peptides/mL. A protective role to induced oxidative stress was observed. The transepithelial transport assays indicate high apparent permeability coefficient (Papp) values for BSY peptides across Caco-2/HT29-MTX cell monolayer (14.5-26.1 × 10-6cm/s) and for Caco-2 cell monolayer model (12.4-20.8 × 10-6cm/s), while the antioxidant and ACE-inhibitory activities found in flux material from the basolateral side suggest transepithelial absorption of bioactive compounds.
LAQV/REQUIMTE, Departamento de Ciências Químicas, Laboratório de Bromatologia e Hidrologia, Faculdade de Farmácia, Universidade do Porto , 4050-313, Porto, Portugal.
Elsa F
EF
Vieira
i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto , 4200-135, Porto, Portugal.|INEB-Instituto de Engenharia Biomédica, Universidade do Porto , 4200-135, Porto, Portugal.|CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde & Instituto Universitário de Ciências da Saúde , 4585-116, Gandra, Portugal.
José
J
das Neves
iBiMED-Instituto de Biomedicina da Universidade de Aveiro , 3810-193, Aveiro, Portugal.|Departamento de Fisiologia e Cirurgia Cardiotorácica da Faculdade de Medicina, Universidade do Porto , 4200-319, Porto, Portugal.
Rui
R
Vitorino
UCIBIO/REQUIMTE, Departamento de Ciências Biológicas, Laboratório de Toxicologia, Faculdade de Farmácia, Universidade do Porto , Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.
Diana
D
Dias da Silva
UCIBIO/REQUIMTE, Departamento de Ciências Biológicas, Laboratório de Toxicologia, Faculdade de Farmácia, Universidade do Porto , Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.
Helena
H
Carmo
LAQV/REQUIMTE, Departamento de Ciências Químicas, Laboratório de Bromatologia e Hidrologia, Faculdade de Farmácia, Universidade do Porto , 4050-313, Porto, Portugal.
Isabel M P L V O
IM
Ferreira
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D001692:Biological Transport; D018938:Caco-2 Cells; D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D018920:Coculture Techniques; D004063:Digestion; D005656:Fungal Proteins / Q000378:metabolism*; D065569:Gastrointestinal Absorption; D006801:Humans; D010455:Peptides / Q000737:chemistry; D010539:Permeability; D011492:Protein Hydrolysates / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000806:Angiotensin-Converting Enzyme Inhibitors; D000975:Antioxidants; D005656:Fungal Proteins; D010455:Peptides; D011492:Protein Hydrolysates
ACE-inhibitory activity; Brewer’s spent yeast; antioxidant activity; gastrointestinal digestion; transepithelial transport