[Regulatory effect of microRNA-141 on synthesis of high mobility group protein B1 in mouse carcinoma cell line hepal-6].
OBJECTIVE
To explore the regulatory effect of microRNA-141 (miR-141) on expression of high mobility group protein B1 (HMGB1) in mouse hepatoma carcinoma cell line (hepal-6 cells).


METHODS
The hepal-6 cells were divided into 4 groups: a miR-141 mimic group, a miR-141 mimic control group, a miR-141 inhibitor group and a miR-141 inhibitor control group. The miR-141 was transfected into hepal-6 cells with lipofectamine 2000. The levels of miR-141 and HMGB1 mRNA in the hepal-6 cells were detected by quantitative real-time PCR (qRT-PCR), and then HMGB1 protein was examined by Western blot. The regulatory effect of miR-141 on 3'-UTR of candidate target gene (HMGB1) was determined by dual-luciferase reporter assay.


RESULTS
Compared with the control group, the level of miR-141 was up-regulated in the miR-141 mimic group, while down-regulated in the miR-141 inhibitor group. Moreover, the levels of HMGB1 mRNA and protein decreased in the miR-141 mimic group, while increased in the miR-141 inhibitor group. The dual-luciferase reporter assay showed that miR-141 could target the 3'-UTR of HMGB1 gene.


CONCLUSIONS
MiR-141 can up-regulate the expression of HMGB1 protein at the post-transcriptional level by targeting to the specific sequence of 3'-UTR of HMGB1 gene, which suggests that HMGB1 gene may be a target gene of miR-141.
Department of Hepatopancreatobiliary Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Hongwei
H
Zhu
Department of Endocrinology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Xia
X
Li
Department of Hepatopancreatobiliary Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Zhiqiang
Z
Li
Department of Hepatopancreatobiliary Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Can
C
Yu
Center for Medical Experiments, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Lihua
L
Huang
Department of Hepatopancreatobiliary Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Xiao
X
Yu
D020413:3' Untranslated Regions; D000818:Animals; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D024243:HMGB1 Protein; D008113:Liver Neoplasms; D008156:Luciferases; D051379:Mice; D035683:MicroRNAs; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D015854:Up-Regulation
D020413:3' Untranslated Regions; D024243:HMGB1 Protein; C582808:HMGB1 protein, mouse; D035683:MicroRNAs; D012333:RNA, Messenger; D008156:Luciferases