Differential activation of c‑Fos in the paraventricular nuclei of the hypothalamus and thalamus following myocardial infarction in rats.
Proto-oncogene c‑Fos (c‑Fos) is frequently used to detect a pathogenesis in central nervous system disorders. The present study examined changes in the immunoreactivity of c‑Fos in the paraventricular nucleus of the hypothalamus (PVNH) and paraventricular nucleus of the thalamus (PVNT) following myocardial infarction (MI) in rats. Infarction in the left ventricle was examined by Masson's trichrome staining. Neuronal degeneration was monitored for 56 days after MI using crystal violet and Fluoro‑Jade B histofluorescence staining. Changes in the immunoreactivity of c‑Fos were determined using immunohistochemistry for c‑Fos. The average infarct size of the left ventricle circumference was ~44% subsequent to MI. Neuronal degeneration was not detected in PVNH and PVNT following MI. c‑Fos immunoreactive (+) cells were infrequently observed in the nuclei of the sham‑group. However, the number of c‑Fos+ cells was increased in the nuclei following MI and peaked in the PVNH and PVNT at 3 and 14 days, respectively. The number of c‑Fos+ cells were comparable with the sham group at 56 days after MI. Therefore, MI may induce c‑Fos immunoreactivity in PVNH and PVNT, this increase of c‑Fos expression levels may be associated with the stress that occurs in the brain following MI.
Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea.
Hyun-Jin
HJ
Tae
Department of Emergency Medicine, Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, Gyeonggi 14068, Republic of Korea.
Seung Min
SM
Park
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Jeong Hwi
JH
Cho
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
In Hye
IH
Kim
Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea.
Ji Hyeon
JH
Ahn
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Joon Ha
JH
Park
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Moo-Ho
MH
Won
Department of Physiology, College of Medicine, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea.
Bai Hui
BH
Chen
Department of Physiology, College of Medicine, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea.
Bich-Na
BN
Shin
Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Myoung Cheol
MC
Shin
Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, Chungcheongnam 16890, Republic of Korea.
Choong Hyun
CH
Lee
Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Seongkweon
S
Hong
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Jae-Chul
JC
Lee
Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Jun Hwi
JH
Cho
D000818:Animals; D008297:Male; D020644:Midline Thalamic Nuclei / Q000378:metabolism / Q000473:pathology*; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology*; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism / Q000473:pathology*; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000378:metabolism*; D017207:Rats, Sprague-Dawley
D016760:Proto-Oncogene Proteins c-fos