Polysaccharide enhances Radix Saposhnikoviae efficacy through inhibiting chromones decomposition in intestinal tract.
Vegetative but not reproductive stage of Saposhnikovia divaricate (Turxz.) schischk possesses pharmacological activities. However, our recent study showed that reproductive S. divaricate supplemented with polysaccharide showed evidently elevated pharmacological activities and increased cimifugin content in rat serum. The aims of present study were to assess the influence of polysaccharides on the chromones pharmacological activities in Radix Saposhnikoviae (RS), the dried root of vegetative stage of S. divaricate, and to explore the underlying mechanisms. Only cimifugin was detected in the plasma of chromone treated animals and RS polysaccharide significantly increased the plasma content of cimifugin. It was shown that neither cimifugin absorption nor glycoside components transformation in simulated digestive fluid was affected by RS polysaccharide. However, a significant promotion of transformation of cimifugin to more stable prime-O-glucosylcimifugin (PGCN) by RS polysaccharide, and a protective effect of polysaccharide on chromone components were observed in small intestine solutions. Meanwhile, RS polysaccharide produced a significant elevation of cimifugin and PGCN concentration in vivo. Based on these findings, we concluded that RS polysaccharide could greatly increase the content of cimifugin, which might be related to its degradation-proof effect on cimifugin, via transforming cimifugin to comparatively more stable PGCN and spatial structure protection.
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.|College of Pharmacy, Jinzhou Medical University, Jinzhou 121000, China.
Jing-Ming
JM
Yang
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.|College of Pharmacy, Jinzhou Medical University, Jinzhou 121000, China.
Hua
H
Jiang
School of Nursing, Jinzhou Medical University, Jinzhou 121000, China.
Hong-Liang
HL
Dai
College of Pharmacy, Jinzhou Medical University, Jinzhou 121000, China.
Zi-Wei
ZW
Wang
Department of Biochemistry and Molecular Biology, Jinzhou Medical University, Jinzhou 121000, China.
Gui-Zhi
GZ
Jia
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Xiang-Cai
XC
Meng
D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D002867:Chromones / Q000097:blood / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats
D002867:Chromones; D011134:Polysaccharides