Quantifications of in vivo labeled stem cells based on measurements of magnetic moments.
Cells labeled by super paramagnetic iron-oxide (SPIO) nanoparticles are more easily seen in gradient echo MR images, but it has not been shown that the amount of nanoparticles or the number of cells can be directly quantified from MR images. This work utilizes a previously developed and improved Complex Image Summation around a Spherical or Cylindrical Object (CISSCO) method to quantify the magnetic moments of several clusters of SPIO nanoparticle labeled cells from archived rat brain images. With the knowledge of mass magnetization of the cell labeling agent and cell iron uptake, the number of cells in each nanoparticle cluster can be determined. Using a high pass filter with a reasonable size has little effect on each measured magnetic moment from the CISSCO method. These procedures and quantitative results may help improve the efficacy of cell-based treatments in vivo.
Department of Radiology, Wayne State University, Detroit, MI 48201. Electronic address: kokenymri@gmail.com.
Paul
P
Kokeny
Department of Radiology, Wayne State University, Detroit, MI 48201. Electronic address: yxc16@wayne.edu.
Yu-Chung N
YN
Cheng
The MRI Institute for Biomedical Research, 761 Lucerne Avenue, Waterloo, ON, Canada.
Saifeng
S
Liu
Department of Physics, Wayne State University, Detroit, MI 48201.
He
H
Xie
Department of Neurology, Henry Ford Health System, Detroit, MI 48202.
Quan
Q
Jiang
D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging*; D003287:Contrast Media; D005290:Ferric Compounds; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D053758:Nanoparticles; D051381:Rats; D013234:Stem Cells
D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide
MRI; Magnetic moment; Quantification; Stem cells; Susceptibility