Development of a VLP-based vaccine in silkworm pupae against rabbit hemorrhagic disease virus.
Rabbit hemorrhagic disease virus (RHDV) is the etiological agent behind rabbit hemorrhagic disease (RHD), which is lethal and contagious in rabbits. The virus does not replicate in cell culture and the only commercial inactivated vaccine available is derived from infected rabbit livers. RHDV capsid protein, VP60, is the main antigen comprising the virion. We used a baculovirus-silkworm pupae system to express VP60, which self-assembled into virus-like particles (VLPs) with a similar size and morphology to RHDV. Hemagglutination assays (HAs) showed that VP60 expression levels of VP60 reached as high as 107HA units (HAU) per pupa. A single intramuscular injection with 104HAU of VLPs completely protected rabbits for at least 180days against RHDV challenge, and for at least 360days when the VLPs were emulsified with Freund's complete adjuvant. These data suggest that silkworm pupae can be used to develop VLP-based vaccines which confer durable protection against RHD.
School of Public Health, Shandong University, Jinan 250012, China; China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 10081, China.
Xuexing
X
Zheng
China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 10081, China.
Shukun
S
Wang
China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 10081, China; Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China.
Weijiao
W
Zhang
China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 10081, China.
Xingjian
X
Liu
China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 10081, China.
Yongzhu
Y
Yi
China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 10081, China; Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China.
Songtao
S
Yang
China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 10081, China; Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China.
Xianzhu
X
Xia
China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 10081, China. Electronic address: liyinv@caas.cn.
Yinv
Y
Li
China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 10081, China. Electronic address: zhifangzhang@yahoo.com.
Zhifang
Z
Zhang
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012831:Bombyx / Q000235:genetics / Q000378:metabolism; D017250:Caliciviridae Infections / Q000517:prevention & control* / Q000662:veterinary; D017929:Hemorrhagic Disease Virus, Rabbit / Q000276:immunology*; D011679:Pupa / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D058425:Vaccines, Virus-Like Particle; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014765:Viral Vaccines
D000956:Antigens, Viral; D058425:Vaccines, Virus-Like Particle; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C081149:viral protein 60, rabbit hemorrhagic disease virus
Immunogenicity; RHDV; Recombinant baculoviruses; Virus-like particles