Enhanced plant growth promoting role of phycomolecules coated zinc oxide nanoparticles with P supplementation in cotton (Gossypium hirsutum L.).
This report focuses on application of zinc oxide nanoparticles (ZnONPs) carrying phycomolecule ligands as a novel plant growth promoter aimed at increasing the crop productivity. The present investigation examined the effect of ZnONPs on plant growth characteristics, and associated biochemical changes in cotton (Gossypium hirsutum L.) following growth in a range of concentrations (25-200 mg L-lZnONPs) in combination with 100 mM P in a hydroponic system. Treated plants registered an increase in growth and total biomass by 130.6% and 131%, respectively, over control. Results demonstrated a significant increase in the level of chlorophyll a (141.6%), b (134.7%), carotenoids (138.6%), and total soluble protein contents (179.4%); at the same time, a significant reduction (68%) in the level of malondialdehyde (MDA) in leaves with respect to control. Interestingly, a significant increase in superoxide dismutase (SOD, 264.2%), and peroxidase (POX, 182.8%) enzyme activities followed by a decrease in the catalase (CAT) activity, in response to above treatments. These results suggest that bioengineered ZnONPs interact with meristematic cells triggering biochemical pathways conducive to an accumulation of biomass. Further investigations will map out the mode of action involved in growth promotion.
Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem, 636 011, Tamil Nadu, India. Electronic address: pvenkat67@yahoo.com.
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Venkatachalam
Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem, 636 011, Tamil Nadu, India.
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Priyanka
Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem, 636 011, Tamil Nadu, India.
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Manikandan
Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem, 636 011, Tamil Nadu, India.
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Ganeshbabu
Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem, 636 011, Tamil Nadu, India.
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Indiraarulselvi
Department of Biotechnology, Mother Teresa Women's University, Kodaikanal, 624 102, Tamil Nadu, India.
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Geetha
National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110 012, India.
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Muralikrishna
National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110 012, India.
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Bhattacharya
Department of Biology, Western Kentucky University, Bowling Green, KY, 42101, USA.
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Tiwari
Department of Biology, Western Kentucky University, Bowling Green, KY, 42101, USA.
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Sharma
Department of Biology, Western Kentucky University, Bowling Green, KY, 42101, USA.
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Sahi
D018533:Biomass; D002338:Carotenoids / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D003368:Gossypium / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018527:Hydroponics / Q000379:methods; D008315:Malondialdehyde / Q000378:metabolism; D018519:Meristem / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009195:Peroxidase / Q000378:metabolism; D010758:Phosphorus / Q000737:chemistry / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000378:metabolism; D013499:Surface Properties; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry / Q000494:pharmacology*
D010940:Plant Proteins; D002734:Chlorophyll; D010758:Phosphorus; D002338:Carotenoids; D008315:Malondialdehyde; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D015034:Zinc Oxide
Antioxidant defense enzymes; Cotton; Gossypium hirsutum; Nanoparticles; Phycomolecules; Plant growth promoting compounds