Identification of a Novel HIV Type 1 Circulating Recombinant Form (CRF86_BC) Among Heterosexuals in Yunnan, China.
In recent years, multiple circulating recombinant forms (CRFs) and unique recombinant forms of human immunodeficiency virus type 1 (HIV-1) have been described in Yunnan, China. Here, we identified a novel HIV-1 CRF (CRF86_BC) isolated from three heterosexuals with no obvious epidemiologic linkage in western Yunnan (Baoshan prefecture) in China. CRF86_BC had a subtype C backbone with four subtype B fragments inserted into the pol, vpr, vpu, env, and nef gene regions, respectively. Furthermore, subregion tree analysis revealed that subtype C backbone originated from an Indian C lineage and subtype B segment inserted was from a Thai B lineage. They are different from previously documented B/C forms in its distinct backbone, inserted fragment size, and break points. This highlighted the importance of continual monitoring of genetic diversity and complexity of HIV-1 strains in this region.
1 Pharmaceutical College, Guangxi Medical University , Guangxi, China .|2 Center of Chinese Medicine, Yunnan Traditional Chinese Medicine Institute , Yunnan, China .
Yanping
Y
Li
3 Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
Jing
J
Miao
3 Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
Zhijiang
Z
Miao
3 Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
Yindi
Y
Song
2 Center of Chinese Medicine, Yunnan Traditional Chinese Medicine Institute , Yunnan, China .
Min
M
Wen
2 Center of Chinese Medicine, Yunnan Traditional Chinese Medicine Institute , Yunnan, China .
Ya
Y
Zhang
2 Center of Chinese Medicine, Yunnan Traditional Chinese Medicine Institute , Yunnan, China .
Shimin
S
Guo
2 Center of Chinese Medicine, Yunnan Traditional Chinese Medicine Institute , Yunnan, China .
Yuan
Y
Zhao
3 Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
Yue
Y
Feng
3 Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
Xueshan
X
Xia
D000328:Adult; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D019143:Evolution, Molecular; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D020010:Heterosexuality; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
HIV-1; Yunnan; epidemiology; recombinant