SWATH-MS Quantitative Proteomic Investigation Reveals a Role of Jasmonic Acid during Lead Response in Arabidopsis.
Lead (Pb) pollution is a growing environment problem that continuously threatens the productivity of crops. To understand the molecular mechanisms of plant adaptation to Pb toxicity, we examined proteome changes in Arabidopsis seedlings following Pb treatment by SWATH-MS, a label-free quantitative proteomic platform. We identified and quantified the expression of 1719 proteins in water- and Pb-treated plants. Among them, 231 proteins showed significant abundance changes (151 elevated and 80 reduced) upon Pb exposure. Functional categorization revealed that most of the Pb-responsive proteins are involved in different metabolic processes. For example, down-regulation of photosynthesis and biosynthesis of isoprenoids and tetrapyrroles in chloroplasts were observed. On the contrary, pathways leading to glutathione, jasmonic acid (JA), glucosinolate (GSL), and phenylpropanoid production are up-regulated. Experimental characterizations demonstrated a rapid elevation of endogenic JA production in Pb-treated Arabidopsis seedlings, while a JA-deficient mutant and a JA-insensitive mutant showed hypersensitivity to root inhibition by Pb, implicating an essential role of JA during Pb responses. Consistently, methyl jasmonate supplementation alleviated Pb toxicity in the wild-type and JA-deficient mutant. Furthermore, GSL levels were substantially enhanced following Pb treatment, while such induction was not detected in the JA mutant, suggesting that the Pb-induced GSL accumulation is JA-dependent. Overall, our work represents the first SWATH-MS analysis in Arabidopsis and highlights a potential mediating role of JA during Pb stress.
School of Biological Sciences, The University of Hong Kong , Pokfulam Road, Hong Kong, China.|School of Life Sciences, The Chinese University of Hong Kong , Shatin, Hong Kong, China.|Shenzhen Research Institute, The Chinese University of Hong Kong , Shenzhen, China.
Fu-Yuan
FY
Zhu
School of Biological Sciences, The University of Hong Kong , Pokfulam Road, Hong Kong, China.
Wai-Lung
WL
Chan
School of Life Sciences, The Chinese University of Hong Kong , Shatin, Hong Kong, China.
Mo-Xian
MX
Chen
SCIEX , Hong Kong, China.
Ricky P W
RP
Kong
Department of Horticulture, Zhejiang University , Hangzhou 310058, China.
Congxi
C
Cai
Department of Horticulture, Zhejiang University , Hangzhou 310058, China.
Qiaomei
Q
Wang
School of Life Sciences, The Chinese University of Hong Kong , Shatin, Hong Kong, China.
Jian-Hua
JH
Zhang
School of Biological Sciences, The University of Hong Kong , Pokfulam Road, Hong Kong, China.
Clive
C
Lo
D000220:Adaptation, Biological / Q000187:drug effects; D017360:Arabidopsis / Q000502:physiology*; D003517:Cyclopentanes / Q000494:pharmacology*; D004785:Environmental Pollutants / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D005961:Glucosinolates / Q000378:metabolism; D007854:Lead / Q000494:pharmacology*; D013058:Mass Spectrometry; D054883:Oxylipins / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis / Q000187:drug effects; D040901:Proteomics / Q000379:methods*; D036226:Seedlings / Q000502:physiology
D003517:Cyclopentanes; D004785:Environmental Pollutants; D005961:Glucosinolates; D054883:Oxylipins; D020543:Proteome; D007854:Lead; C011006:jasmonic acid
Arabidopsis; SWATH; glucosinolates; jasmonic acid; lead response