Abrogation of MUC5AC Expression Contributes to the Apoptosis and Cell Cycle Arrest of Colon Cancer Cells.
Deregulated expressions of mucins have been found in various malignancies and play a pivotal role in carcinogenesis. MUC5AC, as a secreted mucin, is reported to be aberrantly expressed during epithelial cancer progression, including colon cancer. However, the mechanisms of the oncoprotein MUC5AC in the initiation of colon cancer requires further investigation. Here, we collected colon cancer tissues (n = 20) and corresponding paracancerous tissues (n = 20) and found that the expression of MUC5AC was significantly elevated in colon cancer tissues when compared with the corresponding paracancerous tissues. Immunofluorescence indicated that all colon cancer cell lines, including HT29, SW620, and the normal human intestinal epithelial cells FHC, showed the positive expression of MUC5AC, and SW620 exhibited the highest expression. Moreover, knockdown of MUC5AC in SW620 cells remarkably suppressed cell vitality and promoted apoptosis and G1 cell cycle arrest, resulting in the impaired ability of colony formation. Furthermore, the inhibition of MUC5AC in SW620 cells dramatically repressed the cell migration and invasion. These results demonstrated that MUC5AC as an oncogene could be a promising target in the treatment of colon cancer.
Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guilin Medical University , Guilin City, China .
Xijia
X
Zhu
Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guilin Medical University , Guilin City, China .
Xiangkai
X
Long
Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guilin Medical University , Guilin City, China .
Xishun
X
Luo
Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guilin Medical University , Guilin City, China .
Zhike
Z
Song
Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guilin Medical University , Guilin City, China .
Shengguo
S
Li
Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Guilin Medical University , Guilin City, China .
Haipeng
H
Wang
D017209:Apoptosis / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D059585:G1 Phase Cell Cycle Checkpoints / Q000502:physiology; D019073:HT29 Cells; D006801:Humans; D055271:Mucin 5AC / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D014162:Transfection
C524678:MUC5AC protein, human; D055271:Mucin 5AC; D034741:RNA, Small Interfering
MUC5AC; apoptosis; cell proliferation; colon cancer; metastasis