Effects of high organic load on amoA and nirS gene diversity of an intermittently aerated and fed membrane bioreactor treating landfill leachate.
The effects of external carbon source addition on the nitrification and denitrification process were investigated in an intermittently aerated and fed membrane bioreactor treating landfill leachate by recording system performance, and amoA and nirS diversity dynamics using pyrosequencing. By adding 950mg/L glycerol, denitrification was optimized, resulting in total nitrogen removal efficiency of 81.0±2.4%. Under these conditions, amoA diversity was dominated by genotypes related to Nitrosomonas europaea, while increase in leachate's content and in glycerol addition by 50% led to irreversible inhibition of nitrification and enhanced ammonia accumulation, causing a severe suppression of Nitrosomonas and an increase in the relative abundance of Nitrosospira. However, this increase not only affected ammonia oxidizers, but also caused a massive shift in denitrifying community structure, resulting in the suppression of Arenimonas metalli-, Candidatus Accumulibacter- and Sulfuritalea hydrogenivorans-nirS related genotypes and the predominance of nirS-associated with Acidovorax and Thaurea sp.
Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100 Xanthi, Greece.
Nikolaos
N
Remmas
Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100 Xanthi, Greece.
Paraschos
P
Melidis
Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100 Xanthi, Greece.
Efthymia
E
Katsioupi
Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100 Xanthi, Greece. Electronic address: sntougia@env.duth.gr.
Spyridon
S
Ntougias
D000332:Aerobiosis; D001483:Base Sequence; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D005798:Genes, Bacterial; D014644:Genetic Variation; D008567:Membranes, Artificial; D009584:Nitrogen / Q000032:analysis; D009606:Nitrosomonas / Q000235:genetics; D009930:Organic Chemicals / Q000032:analysis*; D010084:Oxidation-Reduction; D010802:Phylogeny; D010865:Pilot Projects; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
D008567:Membranes, Artificial; D009930:Organic Chemicals; D014874:Water Pollutants, Chemical; D009584:Nitrogen
Ammonia monoxygenase subunit A gene (amoA); Ammonium-rich wastewater; Functional diversity; Landfill leachate; Nitrite reductase gene (nirS)