Prenatal and lactation nicotine exposure affects morphology and function of brown adipose tissue in male rat offspring.
The aim of this study was to investigate the effects of prenatal and lactation nicotine exposure on the morphology and function of brown adipose tissue (BAT) in male rat offspring. We conducted a morphological assay and gene expression study of interscapular BAT (iBAT) in male rat offspring. The male offspring from nicotine-exposed dams exhibited higher body weight and iBAT weight. Hematoxylin and eosin staining and transmission electron microscopy showed that iBAT from nicotine-exposed male offspring presented a "whitening" phenotype characterized by lipid droplet accumulation and impaired mitochondria with a randomly oriented and fractured cristae. The expression of the iBAT structure and function-related genes all decreased in nicotine-exposed male offspring. These data indicate that prenatal and lactation nicotine exposure affects morphology and function of iBAT in male rat offspring.
a Department of Pharmacology , Basic Medical School of Wuhan University , Wuhan , China.
Jie
J
Fan
a Department of Pharmacology , Basic Medical School of Wuhan University , Wuhan , China.
Jie
J
Ping
a Department of Pharmacology , Basic Medical School of Wuhan University , Wuhan , China.
Wan-Xia
WX
Zhang
a Department of Pharmacology , Basic Medical School of Wuhan University , Wuhan , China.
Yi-Song
YS
Rao
a Department of Pharmacology , Basic Medical School of Wuhan University , Wuhan , China.
Han-Xiao
HX
Liu
b The Center for Animal Experiment/Animal Biosafety Level 3 Laboratory , Wuhan University , Wuhan , China.
Jing
J
Zhang
a Department of Pharmacology , Basic Medical School of Wuhan University , Wuhan , China.
You-E
YE
Yan
D002001:Adipose Tissue, Brown / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D007774:Lactation; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D046529:Microscopy, Electron, Transmission; D009538:Nicotine / Q000009:adverse effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome / Q000187:drug effects
D009538:Nicotine
Brown adipose tissue; lactation; nicotine; prenatal