Gene therapy based in antimicrobial peptides and proinflammatory cytokine prevents reactivation of experimental latent tuberculosis.
Mycobacterium tuberculosis (Mtb) latent infection can lead to reactivation. The design of new strategies to prevent it is an important subject. B6D2F1 mice were infected intratracheally with a low dose of Mtb H37Rv to induce chronic infection. After 7 months, mice were treated with one dose of recombinant adenoviruses encoding TNFα, β defensin-3 and LL37. Immunosupression was induced 1 month later with corticosterone. In comparison with the control group, mice treated with adenoviruses showed significantly less bacterial load and pneumonia, the adenoviruses encoding TNFα and LL37 being the most efficient. Gene therapy based in a proinflammatory cytokine or antimicrobial peptides is a potentially useful system to prevent reactivation of latent tuberculosis.
Section of Experimental Pathology, Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, México City, México.
Octavio
O
Ramos-Espinosa
Section of Experimental Pathology, Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, México City, México.
Sujhey
S
Hernández-Bazán
Section of Experimental Pathology, Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, México City, México.
Alejandro
A
Francisco-Cruz
Section of Experimental Pathology, Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, México City, México.
Dulce
D
Mata-Espinosa
Section of Experimental Pathology, Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, México City, México.
Jorge
J
Barrios-Payán
Section of Experimental Pathology, Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, México City, México.
Brenda
B
Marquina-Castillo
Department of Cell Biology, Cellular Physiology Institute, National Autonomous University of México, México City, México.
Fernando
F
López-Casillas
Epithelial Damage, Repair and Tissue Engineering, Ciemat-Fundación Marcelino Botín, Complutense University, Madrid, Spain IIS-Fundación Jiménez Díaz, Madrid, Spain.
Marta
M
Carretero
Epithelial Damage, Repair and Tissue Engineering, Ciemat-Fundación Marcelino Botín, Complutense University, Madrid, Spain IIS-Fundación Jiménez Díaz, Madrid, Spain.
Marcela
M
Del Río
Section of Experimental Pathology, Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, México City, México rhdezpando@hotmail.com.
Rogelio
R
Hernández-Pando
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D015415:Biomarkers; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000378:metabolism; D055985:Latent Tuberculosis / Q000235:genetics* / Q000382:microbiology* / Q000473:pathology / Q000628:therapy; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D023181:Antimicrobial Cationic Peptides; D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators; D014409:Tumor Necrosis Factor-alpha
antimicrobial peptides; experimental models; gene therapy; innate immunity; latent tuberculosis; proinflammatory cytokine