Kinetic Control of Microtubule Morphology Obtained by Assembling Gold Nanoparticles on Living Fungal Biotemplates.
Self-assembly of nanoparticles on living biotemplate surfaces is a promising route to fabricate nano- or microstructured materials with high efficiency and efficacy. We used filamentous fungi to fabricate microtubules of gold nanoparticles through a novel approach that consists of isolating the hyphal growth from the nanoparticle media. This improved methodology resulted in better morphological control and faster adsorption kinetics, which reduced the time needed to form homogeneous microtubules and allowed for control of microtubule thickness through successive additions of nanoparticles. Differences in the adsorption rates due to modifications in the chemical identity of colloidal gold nanoparticles indicated the influence of secondary metabolites and growth media in the fungi metabolism, which demonstrated the need to choose not only the fungus biotemplate but also the correct medium to obtain microtubules with superior properties.
LIEC Interdisciplinary Laboratory of Electrochemistry and Ceramics, Department of Chemistry, UFSCar Federal University of São Carlos , São Carlos, São Paulo, 13.565-905, Brazil.
Andressa M
AM
Kubo
LIEC Interdisciplinary Laboratory of Electrochemistry and Ceramics, Department of Chemistry, UFSCar Federal University of São Carlos , São Carlos, São Paulo, 13.565-905, Brazil.
Luiz F
LF
Gorup
Laboratory of Micromolecular Biochemistry of Microorganisms, Department of Chemistry, UFSCar Federal University of São Carlos , São Carlos, São Paulo, 13.565-905, Brazil.
Luciana S
LS
Amaral
Laboratory of Micromolecular Biochemistry of Microorganisms, Department of Chemistry, UFSCar Federal University of São Carlos , São Carlos, São Paulo, 13.565-905, Brazil.
Edson R
ER
Filho
LIEC Interdisciplinary Laboratory of Electrochemistry and Ceramics, Department of Chemistry, UFSCar Federal University of São Carlos , São Carlos, São Paulo, 13.565-905, Brazil.
Emerson R
ER
Camargo
D001230:Aspergillus / Q000737:chemistry / Q000166:cytology; D005658:Fungi / Q000737:chemistry / Q000166:cytology*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D017348:Microscopy, Electron, Scanning Transmission; D008870:Microtubules / Q000737:chemistry; D010407:Penicillium / Q000737:chemistry / Q000166:cytology; D014961:X-Ray Diffraction; D013098:Xylariales / Q000737:chemistry / Q000166:cytology
D006046:Gold