Quantitative deviating effects of maple syrup extract supplementation on the hepatic gene expression of mice fed a high-fat diet.
Maple syrup contains various polyphenols and we investigated the effects of a polyphenol-rich maple syrup extract (MSXH) on the physiology of mice fed a high-fat diet (HFD).



The mice fed a low-fat diet (LFD), an HFD, or an HFD supplemented with 0.02% (002MSXH) or 0.05% MSXH (005MSXH) for 4 weeks. Global gene expression analysis of the liver was performed, and the differentially expressed genes were classified into three expression patterns; pattern A (LFD < HFD > 002MSXH = 005MSXH, LFD > HFD < 002MSXH = 005MSXH), pattern B (LFD < HFD = 002MSXH > 005MSXH, LFD > HFD = 002MSXH < 005MSXH), and pattern C (LFD < HFD > 002MSXH < 005MSXH, LFD > HFD < 002MSXH > 005MSXH). Pattern A was enriched in glycolysis, fatty acid metabolism, and folate metabolism. Pattern B was enriched in tricarboxylic acid cycle while pattern C was enriched in gluconeogenesis, cholesterol metabolism, amino acid metabolism, and endoplasmic reticulum stress-related event.



Our study suggested that the effects of MSXH ingestion showed (i) dose-dependent pattern involved in energy metabolisms and (ii) reversely pattern involved in stress responses.
Project for Development of Food Functionality Assessment Methods, Kanagawa Academy of Science and Technology, LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, Japan.
Asuka
A
Kamei
Project for Development of Food Functionality Assessment Methods, Kanagawa Academy of Science and Technology, LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, Japan.
Yuki
Y
Watanabe
Project for Development of Food Functionality Assessment Methods, Kanagawa Academy of Science and Technology, LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, Japan.
Fumika
F
Shinozaki
Project for Development of Food Functionality Assessment Methods, Kanagawa Academy of Science and Technology, LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, Japan.
Akihito
A
Yasuoka
Project for Development of Food Functionality Assessment Methods, Kanagawa Academy of Science and Technology, LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, Japan.
Kousuke
K
Shimada
Project for Development of Food Functionality Assessment Methods, Kanagawa Academy of Science and Technology, LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, Japan.
Kaori
K
Kondo
Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
Tomoko
T
Ishijima
Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
Tsudoi
T
Toyoda
Project for Development of Food Functionality Assessment Methods, Kanagawa Academy of Science and Technology, LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, Japan.|General Research Institute, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, Japan.
Soichi
S
Arai
Project for Development of Food Functionality Assessment Methods, Kanagawa Academy of Science and Technology, LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, Japan.
Takashi
T
Kondo
Project for Development of Food Functionality Assessment Methods, Kanagawa Academy of Science and Technology, LiSE 4F C-4, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, Japan.|Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
Keiko
K
Abe
D031002:Acer / Q000737:chemistry*; D000818:Animals; D059305:Diet, High-Fat; D000073417:Dietary Sugars / Q000494:pharmacology; D019587:Dietary Supplements; D005227:Fatty Acids / Q000378:metabolism; D005786:Gene Expression Regulation; D008099:Liver / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008810:Mice, Inbred C57BL
D000073417:Dietary Sugars; D005227:Fatty Acids
Gene expression analysis; High-fat diet; Liver; Maple syrup; Polyphenol