Organic Nanoparticles with Aggregation-Induced Emission for Bone Marrow Stromal Cell Tracking in a Rat PTI Model.
Stem-cell based therapy is an emerging therapeutic approach for ischemic stroke treatment. Bone marrow stromal cells (BMSCs) are in common use as a cell source for stem cell therapy and show promising therapeutic outcomes for stroke treatment. One challenge is to develop a reliable tracking strategy to monitor the fate of BMSCs and assess their therapeutic effects in order to improve the success rate of such treatment. Herein, TPEEP, a fluorogen with aggregation-induced emission characteristics and near-infrared emission are designed and synthesized and further fabricated into organic nanoparticles (NPs). The obtained NPs show high fluorescence quantum yield, low cytotoxicity with good physical and photostability, which display excellent tracking performance of BMSCs in vitro and in vivo. Using a rat photothrombotic ischemia model as an example, the NP-labeled BMSCs are able to migrate to the stroke lesion site to yield bright red fluorescence. Immunofluorescence staining shows that the NP labeling does not affect the normal function of BMSCs, proving their good biocompatibility in vivo. These merits make TPEEP NP a potential cell tracker to evaluate the fate of BMSCs in cell therapy.
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585.|NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, 28 Medical Drive, #05-01, Singapore, 117456.
Xiaolei
X
Cai
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585.
Chong-Jing
CJ
Zhang
Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, 28 Medical Drive, Singapore, 117456.
Frances
F
Ting Wei Lim
Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, 2129, USA.
Su Jing
SJ
Chan
Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, 28 Medical Drive, Singapore, 117456.
Aishwarya
A
Bandla
Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, 28 Medical Drive, Singapore, 117456.
Chan Kim
CK
Chuan
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585.
Fang
F
Hu
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585.
Shidang
S
Xu
Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, 28 Medical Drive, Singapore, 117456.
Nitish V
NV
Thakor
Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, 28 Medical Drive, Singapore, 117456.|Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan.
Lun-De
LD
Liao
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585.
Bin
B
Liu
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D002545:Brain Ischemia / Q000000981:diagnostic imaging*; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats
D005456:Fluorescent Dyes
aggregation-induced emission (AIE); bone marrow stromal cells; cell tracking; nanoparticles; photothrombotic ischemia