Screening and identifying antioxidants from Oplopanax elatus using 2,2'-diphenyl-1-picrylhydrazyl with off-line two-dimensional HPLC coupled with diode array detection and tandem time-of-flight mass spectrometry.
The root of Oplopanax elatus (Nakai) Nakai has a well-known history of use for the treatment of diseases such as neurasthenia, cardiovascular disorders, and cancer by the native people in northeast China. It is important to screen and identify the bioactive molecules from its root rapidly. Hereby, an off-line two-dimensional high performance liquid chromatography coupled with diode array detection and tandem time-of-flight mass spectrometry together with 2,2'-diphenyl-1-picrylhydrazyl was established to screen antioxidants from the root of O. elatus. A Waters cyanogen column (150 × 3.9 mm, id, 4 μm) was used for the first dimensional liquid chromatography, while a Hypersil BDS-C18 column (250 × 4.6 mm, id, 5 μm) was installed for the second dimension liquid chromatographic analysis. Twenty-eight compounds had been tentatively identified from the methanol extract of the air-dried root of O. elatus including six polyynes and eight phenolic derivatives were screened with antioxidant activity. The developed method could be expedient for screening and identifying antioxidants from O. elatus.
Department of Pharmacognosy, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China.
Li
L
Shao
Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, China.|Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha, China.
Ming-Kun
MK
Nie
Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, China.|Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha, China.
Man-Yun
MY
Chen
Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, China.|Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha, China.
Jin
J
Wang
Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL, USA.
Chong-Zhi
CZ
Wang
Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, China.|Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha, China.|Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL, USA.
Wei-Hua
WH
Huang
Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL, USA.
Chun-Su
CS
Yuan
Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, China.|Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha, China.
Hong-Hao
HH
Zhou
D000975:Antioxidants / Q000032:analysis*; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D028002:Oplopanax / Q000737:chemistry*; D010851:Picrates; D018517:Plant Roots / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D000975:Antioxidants; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl
Antioxidants; Oplopanax; Oplopanax elatus; Phenolics