Detection of regional DNA methylation using DNA-graphene affinity interactions.
We report a new method for the detection of regional DNA methylation using base-dependent affinity interaction (i.e., adsorption) of DNA with graphene. Due to the strongest adsorption affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads to a larger amount of the adsorbed DNA on the graphene-modified electrodes in comparison to the adenine-enriched unmethylated DNA. The level of the methylation is quantified by monitoring the differential pulse voltammetric current as a function of the adsorbed DNA. The assay is sensitive to distinguish methylated and unmethylated DNA sequences at single CpG resolution by differentiating changes in DNA methylation as low as 5%. Furthermore, this method has been used to detect methylation levels in a collection of DNA samples taken from oesophageal cancer tissues.
Cancer Molecular Pathology Laboratory in School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Australia; School of Natural Sciences, Griffith University, Nathan Campus, QLD 4111, Australia.
Md Hakimul
MH
Haque
Cancer Molecular Pathology Laboratory in School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Australia. Electronic address: v.gopalan@griffith.edu.au.
Vinod
V
Gopalan
School of Natural Sciences, Griffith University, Nathan Campus, QLD 4111, Australia; Queensland Micro and Nanotechnology Centre, Griffith University, Nathan Campus, QLD 4111, Australia.
Sharda
S
Yadav
School of Natural Sciences, Griffith University, Nathan Campus, QLD 4111, Australia; Queensland Micro and Nanotechnology Centre, Griffith University, Nathan Campus, QLD 4111, Australia.
Md Nazmul
MN
Islam
Queensland Micro and Nanotechnology Centre, Griffith University, Nathan Campus, QLD 4111, Australia; School of Engineering, Griffith University, Nathan, QLD 4111, Australia.
Ehsan
E
Eftekhari
Queensland Micro and Nanotechnology Centre, Griffith University, Nathan Campus, QLD 4111, Australia; School of Engineering, Griffith University, Nathan, QLD 4111, Australia.
Qin
Q
Li
The University of Queensland, Brisbane, QLD 4072, Australia.
Laura G
LG
Carrascosa
Queensland Micro and Nanotechnology Centre, Griffith University, Nathan Campus, QLD 4111, Australia.
Nam-Trung
NT
Nguyen
Cancer Molecular Pathology Laboratory in School of Medicine, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Australia. Electronic address: a.lam@griffith.edu.au.
Alfred K
AK
Lam
School of Natural Sciences, Griffith University, Nathan Campus, QLD 4111, Australia; Queensland Micro and Nanotechnology Centre, Griffith University, Nathan Campus, QLD 4111, Australia. Electronic address: m.shiddiky@griffith.edu.au.
Muhammad J A
MJA
Shiddiky
D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D018899:CpG Islands; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D019175:DNA Methylation; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D004938:Esophageal Neoplasms / Q000235:genetics; D004947:Esophagus / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006147:Guanine / Q000032:analysis; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins / Q000235:genetics
D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C522691:RETREG1 protein, human; D006147:Guanine; D006108:Graphite; D004247:DNA
DNA methylation; Disposable screen-printed electrode; Electrochemical detection; Graphene-DNA base interaction; Regional methylation