Identification of SET Domain-Containing Proteins in Gossypium raimondii and Their Response to High Temperature Stress.
SET (Su(var), E(z), and Trithorax) domain-containing proteins play an important role in plant development and stress responses through modifying lysine methylation status of histone. Gossypium raimondii may be the putative contributor of the D-subgenome of economical crops allotetraploid G. hirsutum and G. barbadense and therefore can potentially provide resistance genes. In this study, we identified 52 SET domain-containing genes from G. raimondii genome. Based on conserved sequences, these genes are grouped into seven classes and are predicted to catalyze the methylation of different substrates: GrKMT1 for H3K9me, GrKMT2 and GrKMT7 for H3K4me, GrKMT3 for H3K36me, GrKMT6 for H3K27me, but GrRBCMT and GrS-ET for nonhistones substrate-specific methylation. Seven pairs of GrKMT and GrRBCMT homologous genes are found to be duplicated, possibly one originating from tandem duplication and five from a large scale or whole genome duplication event. The gene structure, domain organization and expression patterns analyses suggest that these genes' functions are diversified. A few of GrKMTs and GrRBCMTs, especially for GrKMT1A;1a, GrKMT3;3 and GrKMT6B;1 were affected by high temperature (HT) stress, demonstrating dramatically changed expression patterns. The characterization of SET domain-containing genes in G. raimondii provides useful clues for further revealing epigenetic regulation under HT and function diversification during evolution.
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P. R. China.
Yong
Y
Huang
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P. R. China.
Yijia
Y
Mo
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P. R. China.
Pengyun
P
Chen
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P. R. China.
Xiaoling
X
Yuan
Key laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P. R. China.
Funing
F
Meng
Key laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P. R. China.
Shengwei
S
Zhu
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P. R. China.
Zhi
Z
Liu
D001483:Base Sequence; D002874:Chromosome Mapping; D017124:Conserved Sequence; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D006358:Hot Temperature; D008745:Methylation; D005810:Multigene Family; D000074463:PR-SET Domains; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological
D010940:Plant Proteins