Prevalence of Leishmania tropica in school boys of khyber agency, FATA near Pak-Afghan border.
In Pakistan leishmaniasis occurs periodically throughout the year and various out breaks are reported frequently. In continuation of our research on this neglected disease, the aim of present study is to explore: (1) the prevalence of cutaneous leishmaniasis in school boys; (2) Leishmania species identification in order to epidemiology and dynamics of the disease; (3) Identification of risk factors for Leishmaniasis especially for CL. The data was collected in August 2014 The experimental strategy involved a questionnaire for data collection and along with clinical diagnosis of 134 out of 9368 students for incidence of Leishmania spp. in 7 square kilometres area in the schoolboys at Tehsil Landi Kotal, District Khyber Agency, FATA Pakistan. The parasitological and molecular diagnosis of clinically suspected cutaneous leishmaniasis cases were performed using microscopical examination of Giemsa-stained smears of lesion exudates and minicircle kDNA semi nested PCR, respectively. Microscopy (x=1000) positive cases were 84/134 (62.6%) and 50/134 (37.4%) slides did not showed any presence of amastigotes of Leishmania spp. The samples were amplified using kDNA semi nested PCR and confirmed the presence of L. Tropica (Ac.no KT 985473). PCR positive cases were 97/134 (72.4%) and 37(27.6%) were negative. The prevalence of L. tropica in school boys was 1.4% (134/9368) in the total population studied (n=9368). The parasite prevalence might be greater as only male students were considered in the study due to ethical and social issues and limitations.
Department of Animal Sciences, Parasitology Laboratory, Quaid-i-Azam University, Islamabad, 45320, Pakistan. Electronic address: naveedaquresh@gmail.com.
Naveeda Akhtar
NA
Qureshi
Department of Animal Sciences, Parasitology Laboratory, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
Abid
A
Ali
Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad, 22060, Pakistan.
Umer
U
Rashid
Department of Microbiology, Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan.
Tayyab-Ur-Rehman
Department of Microbiology, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
Naeem
N
Ali
D000293:Adolescent; D002648:Child; D002662:Child Health Services; D002675:Child, Preschool; D018105:DNA, Kinetoplast / Q000235:genetics; D006801:Humans; D007895:Leishmania tropica / Q000235:genetics / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000453:epidemiology* / Q000469:parasitology; D008297:Male; D010154:Pakistan / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012574:Schools
D018105:DNA, Kinetoplast
Cutaneous leishmaniasis; Epidemological studies; Kinetoplast DNA; PCR