Improving cytoactive of endothelial cell by introducing fibronectin to the surface of poly L-Lactic acid fiber mats via dopamine.
A simple but straightforward approach was reported to prepare fiber mats modified with fibronectin (Fn) protein for endothelial cells activity study. Based on the self-polymerization and strong adhesion feature of dopamine, poly L-Lactic acid (PLLA) fibers mat was modified via simply immersing them into dopamine solution for 16h. Subsequently, Fn was immobilized onto the fiber mats surface by the coupling reactive polydopamine (PDA) layer and Fn. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) were used to determine the chemical compositions of fiber mats surface, which confirmed the successful immobilization of PDA and Fn molecules on the fiber surface. Scanning electronic microscopy (SEM) was used to observe the surface morphology changes after modification with PDA and Fn. The data of water contact angle showed that the hydrophilicity of the fiber mats was improved after surface modification. The data of in vitro cell culture proved that the PDA and Fn modified surface significantly enhanced the adhesion, proliferation and cell activity of endothelial cells on the fiber mats. And the release of tumor necrosis factor-α (TNF-α) by endothelial cells on the modified surface was suppressed compared to that on culture plate and PLLA film at 2 and 4days, while the secretion of interleukin-1β (IL-1β) was increased compared to that on culture plate and PLLA film at 2days.
Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China.
Wufeng
W
Yang
Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China.
Xiazhi
X
Zhang
Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China.
Keke
K
Wu
Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China.
Xiaoyan
X
Liu
Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China. Electronic address: tjiaoyp@jnu.edu.cn.
Yanpeng
Y
Jiao
Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China.
Changren
C
Zhou
D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D007211:Indoles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D001672:Biocompatible Materials; D005353:Fibronectins; D055767:Immobilized Proteins; D007211:Indoles; D011091:Polyesters; D011108:Polymers; C568283:polydopamine; C033616:poly(lactide)
Dopamine; Electrospun fiber mats; Endothelial cells; Fibronectin; Inflammation cytokines; Surface modification