Highly Pathogenic Eurasian H5N8 Avian Influenza Outbreaks in Two Commercial Poultry Flocks in California.
In January 2015, a highly pathogenic Eurasian lineage H5N8 avian influenza (AI) virus (AIV) was detected in a commercial meat turkey flock in Stanislaus County, CA. Approximately 3 wk later, a similar case was diagnosed in commercial brown layers from a different company located in Kings County, CA. Five 14-wk-old turkey hens were submitted to the California Animal Health and Food Safety Laboratory System (CAHFS), Turlock, and eleven 12-wk-old chickens were submitted to CAHFS, Tulare laboratory due to an acute increase in flock mortality. Gross lesions included enlarged and mottled pale spleens and pancreas in turkeys and chickens. Histologically, the major lesions observed in turkeys and chickens were splenitis, pancreatitis, encephalitis, and pneumonia. In both cases, diagnosis was based on real-time reverse transcriptase PCR (RRT-PCR), sequencing, and virus isolation from oropharyngeal and cloacal swabs. Confirmatory diagnosis and AIV characterization was done at the National Veterinary Services Laboratory, Ames, IA. The sequence of the AIV from both cases was 99% identical to an H5N8 AI virus (A/gyrfalcon/Washington/41088-6/2014) isolated from a captive gyrfalcon (Falco rusticolus) from Washington State in December 2014. Immunohistochemistry (IHC) performed on various tissues from both cases indicated a widespread AIV tissue distribution. Except for minor variations, the tissue distribution of the AI antigen was similar in the chickens and turkeys. There was positive IHC staining in the brain, spleen, pancreas, larynx, trachea, and lungs in both chickens and turkeys. Hearts, ovaries, and air sacs from the turkeys were also positive for the AI antigen. The liver sections from the chickens had occasional AI-positive staining in mononuclear cells, but the IHC on liver sections from the turkeys were negative. The bursa of Fabricius, small intestine, kidney, and skeletal muscle sections were negative for the AI antigen in both chickens and turkeys.
A California Animal Health and Food Safety Laboratory System (CAHFS), University of California, Davis, Turlock Branch, 1550 N. Soderquist Road, Turlock, CA 95381.
Simone
S
Stoute
B CAHFS, University of California, Davis, Tulare Branch, 18830 Road 112, Tulare, CA 93274.
Richard
R
Chin
C CAHFS, University of California, Davis, Davis Branch, One Shields Avenue, Davis, CA 95616.
Beate
B
Crossley
A California Animal Health and Food Safety Laboratory System (CAHFS), University of California, Davis, Turlock Branch, 1550 N. Soderquist Road, Turlock, CA 95381.
C
C
Gabriel Sentíes-Cué
A California Animal Health and Food Safety Laboratory System (CAHFS), University of California, Davis, Turlock Branch, 1550 N. Soderquist Road, Turlock, CA 95381.
Arthur
A
Bickford
D Southeast Poultry Research Laboratory, United States National Poultry Research Center, United States Department of Agriculture, Agricultural Research Service, 934 College Station Road, Athens, GA 30605.
Mary
M
Pantin-Jackwood
C CAHFS, University of California, Davis, Davis Branch, One Shields Avenue, Davis, CA 95616.
Richard
R
Breitmeyer
E California Department of Food and Agriculture, Animal Health and Food Safety Services, 2800 Gateway Oaks, Sacramento, CA 95814.
Annette
A
Jones
A California Animal Health and Food Safety Laboratory System (CAHFS), University of California, Davis, Turlock Branch, 1550 N. Soderquist Road, Turlock, CA 95381.
Silvia
S
Carnaccini
B CAHFS, University of California, Davis, Tulare Branch, 18830 Road 112, Tulare, CA 93274.
H L
HL
Shivaprasad
D000818:Animals; D002140:California / Q000453:epidemiology; D002645:Chickens; D004196:Disease Outbreaks / Q000662:veterinary*; D005260:Female; D000071225:Influenza A Virus, H5N8 Subtype / Q000502:physiology*; D005585:Influenza in Birds / Q000175:diagnosis / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D011201:Poultry Diseases / Q000175:diagnosis / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D014422:Turkeys
California; avian influenza; chickens; commercial; highly pathogenic; turkeys