Identification of the Plant Ribokinase and Discovery of a Role for Arabidopsis Ribokinase in Nucleoside Metabolism.
Ribose can be used for energy or as a component of several important biomolecules, but for it to be used in either capacity it must first be phosphorylated by ribokinase (RBSK). RBSK proteins are part of the phosphofructokinase-B (pfkB) family of carbohydrate kinases. Sequence comparisons of pfkB proteins from the model plant Arabidopsis thaliana with the human and Escherichia coli RBSK identified a single candidate RBSK, At1g17160 (AtRBSK). AtRBSK is more similar to predicted RBSKs from other plant species and known mammalian and prokaryotic RBSK than to all other PfkB proteins in Arabidopsis AtRBSK contains a predicted chloroplast transit peptide, and we confirmed plastid localization using AtRBSK fused to YFP. Structure prediction software verified that the AtRBSK sequence mapped onto a known RBSK structure. Kinetic parameters of purified recombinant AtRBSK were determined to be Kmribose= 150 μm ± 17 μm, KmATP= 45 μm ± 5.6 μm, and kcat= 2.0 s-1Substrate inhibition was observed for AtRBSK (KiATP= 2.44 mm ± 0.36 mm), as has been demonstrated for other RBSK proteins. Ribose accumulated in Arabidopsis plants lacking AtRBSK. Such plants grew normally unless media was supplemented with ribose, which led to chlorosis and growth inhibition. Both chlorosis and ribose accumulation were abolished upon the introduction of a transgene expressing AtRBSK-MYC, demonstrating that the loss of protein is responsible for ribose hypersensitivity. Ribose accumulation in plants lacking AtRBSK was reduced in plants also deficient in the nucleoside ribohydrolase NSH1, linking AtRBSK activity to nucleoside metabolism.
From the Department of Molecular and Cellular Biology, University of California, Davis, California 95616.
John W
JW
Riggs
From the Department of Molecular and Cellular Biology, University of California, Davis, California 95616.
Nathan C
NC
Rockwell
From the Department of Molecular and Cellular Biology, University of California, Davis, California 95616.
Philip C
PC
Cavales
From the Department of Molecular and Cellular Biology, University of California, Davis, California 95616 jcallis@ucdavis.edu.
Judy
http://orcid.org/0000-0002-0622-078X
J
Callis
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006801:Humans; D009705:Nucleosides / Q000235:genetics / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D012266:Ribose / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D009705:Nucleosides; D012266:Ribose; D017853:Phosphotransferases (Alcohol Group Acceptor); C022534:ribokinase
carbohydrate kinase; carbohydrate metabolism; carbohydrate processing; enzyme; nucleoside/nucleotide metabolism; phosphorylation; plant biochemistry