Patulibacter brassicae sp. nov., isolated from rhizosphere soil of Chinese cabbage (Brassica campestris).
A novel actinobacterial strain, designated SDT, was isolated from rhizosphere soil of Chinese cabbage in Shandong province, China. The cells were aerobic, Gram-staining-positive, oxidase- and catalase-positive, short rods and formed white colonies on trypticase soy agar. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and alanine, glutamic acid and leucine. Diphosphatidylglycerol was the predominant polar lipid. The predominant cellular fatty acid was C18 : 1ω9c; minor components were anteiso-C15 : 0 and anteiso-C17 : 0. The only isoprenoid quinone was demethylmenaquinone 7 (DMK-7), and the DNA G+C content was 72.7 mol%. Based on the full-length 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of strain SDT were Patulibacter medicamentivorans DSM 25962T (96.9 %), Patulibacter minatonensis DSM 18081T (96.7 %), Patulibacter americanus DSM 16676T (96.2 %) and Patulibacter ginsengiterrae DSM 25990T (95.9 %). Considering combined phenotypic and genotypic characteristics, it is proposed that strain SDT represents a novel species of the genus Patulibacter, for which the name Patulibacter brassicae sp. nov. is proposed. The type strain is SDT (=CICC 24108T=KCTC 39817T).
CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.
Decai
D
Jin
CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.
Xiao
X
Kong
College of Agriculture, Guangxi University, Nanning 530004, PR China.
Honghong
H
Li
Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha 410125, PR China.
Luyun
L
Luo
CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.|College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, PR China.
Xuliang
X
Zhuang
CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.|College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, PR China.
Guoqiang
G
Zhuang
CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.
Ye
Y
Deng
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, PR China.|CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.
Zhihui
Z
Bai
D015373:Bacterial Typing Techniques; D001482:Base Composition; D001937:Brassica / Q000382:microbiology*; D002308:Cardiolipins / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D002308:Cardiolipins; D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7