The complete sequence and genome organization of ligustrum virus A, a novel carlavirus.
The complete genome sequence of ligustrum virus A (LVA) from a Ligustrum obtusifolium Sieb. & Zucc. plant was determined. The genomic RNA has 8,525 nucleotides, excluding the poly(A) tail, and consists of six open reading frames typical of members of the genus Carlavirus, family Betaflexiviridae. Phylogenetic analysis of the viral replicase and coat protein (CP) indicated that LVA is closely related to daphne virus S and helenium virus. The replicase and CP of LVA shared 44.73-52.35 % and 25.39-62.46 % amino acid identity, respectively, with those of other carlaviruses. These results suggest that LVA is a member of a distinct carlavirus species.
Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 34113, Republic of Korea.|Molecular Biofarming Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Davaajargal
D
Igori
Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 34113, Republic of Korea.|Molecular Biofarming Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Seungmo
S
Lim
Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 34113, Republic of Korea.|Molecular Biofarming Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Fumei
F
Zhao
Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 34113, Republic of Korea.|Molecular Biofarming Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Dasom
D
Baek
Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 34113, Republic of Korea.|Molecular Biofarming Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Jeong Mee
JM
Park
Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 34113, Republic of Korea.|Molecular Biofarming Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Hye Sun
HS
Cho
Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 34113, Republic of Korea.|Molecular Biofarming Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Hyun Soon
HS
Kim
Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 34113, Republic of Korea. sykwon@kribb.re.kr.|Molecular Biofarming Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea. sykwon@kribb.re.kr.
Suk-Yoon
SY
Kwon
Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 34113, Republic of Korea. jsmoon@kribb.re.kr.|Molecular Biofarming Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea. jsmoon@kribb.re.kr.
Jae Sun
http://orcid.org/0000-0003-0934-1533
JS
Moon
D017788:Carlavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016000:Cluster Analysis; D023061:Gene Order; D016679:Genome, Viral; D031659:Ligustrum / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D012367:RNA, Viral