miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200.
Cervical cancer (CC) is a malignant solid tumor, which is one of the main causes of morbidity and mortality in women. Persistent High-risk human papillomavirus (hrHPV) infection is closely related to cervical cancer and autophagy has been suggested to inhibit viral infections. miRNAs have been reported to regulate autophagy in many solid tumors with many studies implicating miR-224-3p in the regulation of autophagy. In this study, we performed a miRNA microarray analysis on CC tissues and found that a large number of miRNAs with differential expressions in hrHPV-infected tissues. We identified miR-224-3p as a candidate miRNA selectively up regulated in HPV-infected tissues and cell lines. Further analysis revealed that miR-224-3p regulates autophagy in cervical cancer tissues and cell lines. While the overexpression of miR-224-3p inhibits autophagy in HPV-infected cells, knocking down endogenous miR-224-3p increases autophagy activity in the same cells. In addition, we found that miR-224-3p directly inhibits the expression of autophagy related gene, FAK family-interacting protein of 200 kDa (FIP200). In summary, we found that miR-224-3p regulates autophagy in hrHPV-induced cervical cancer cells through targeting FIP200 expression.
The Second Hospital of Tianjin Medical University, 300070 Tianjin, China.
Wang
W
Fang
The Second Hospital of Tianjin Medical University, 300070 Tianjin, China.
Shan
S
Shu
Department of Medical Microbiology, School of Basic Medical Sciences, Tianjin Medical University, 300070 Tianjin, China.
Li
L
Yongmei
Key Laboratory of Hormones and Development (Ministry of Health), Metabolic Diseases Hospital &Tianjin Institute of Endocrinology, Department of physiology, Tianjin Medical University, 300070 Tianjin, China.
Zhu
Z
Endong
The Second Hospital of Tianjin Medical University, 300070 Tianjin, China.
Yin
Y
Lirong
Key Laboratory of Hormones and Development (Ministry of Health), Metabolic Diseases Hospital &Tianjin Institute of Endocrinology, Department of physiology, Tianjin Medical University, 300070 Tianjin, China.
Sun
S
Bei
D020413:3' Untranslated Regions; D001343:Autophagy; D000071183:Autophagy-Related Proteins; D001483:Base Sequence; D001665:Binding Sites; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D030361:Papillomavirus Infections / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000821:virology
D020413:3' Untranslated Regions; D000071183:Autophagy-Related Proteins; C546140:MIRN224 microRNA, human; D035683:MicroRNAs; C407704:RB1CC1 protein, human; D011505:Protein-Tyrosine Kinases