Immunoprotection of recombinant Eg.myophilin againstEchinococcus granulosusinfection in sheep.
The aims of the present study were to investigate the immunoprotection of recombinantEchinococcus granulosusmyophilin (rEg.myophilin) against the establishment of a challenge oral infection withE. granulosuseggs, as well as to determine the mechanisms underlying this protection. Sheep were subcutaneously immunized two times with rEg.myophilin, followed by the challenge withE. granulosuseggs orally. The animals were sacrificed 44 weeks after infection and the immunoglobulin (Ig) and cytokine levels were analyzed using ELISA. The results identified significant changes in several indexes of animal immune response subsequent to immunization with rEg.myophilin. These changes included reduced number of formed cysts, as well as elevated levels of IgG, IgA and cytokines. The present data suggest that immunization with rEg.myophilin in sheep can successfully reduce the formation of cysts caused by challengeE. granulosusinfection and stimulate immune response, suggesting that rEg.myophilin a has potential value as a candidate vaccine againstE. granulosus.
Center of Scientific Technology, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China; Key Laboratory of Hydatid Disease, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
Mingxing
M
Zhu
Institute of Clinical Laboratory, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China; Xi'an Traditional Chinese Medicine Hospital, Xi'an, Shannxi 710021, P.R. China.
Fu
F
Gao
Center of Scientific Technology, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China; Key Laboratory of Hydatid Disease, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
Zihua
Z
Li
Institute of Clinical Laboratory, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
Xiuqing
X
Wang
Key Laboratory of Hydatid Disease, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China; Department of Pathogenic Biology and Medical Immunology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
Hao
H
Wang
Experimental Animal Center, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
Zhisheng
Z
Wang
Key Laboratory of Hydatid Disease, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China; Department of Pathogenic Biology and Medical Immunology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
Jiaqing
J
Zhao
Center of Scientific Technology, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China; Key Laboratory of Hydatid Disease, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
Wei
W
Zhao
Echinococcus granulosus; recombinant Eg.myophilin; sheep; vaccine