Integrated Amplification Microarrays for Infectious Disease Diagnostics.
This overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber. The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minimizing the potential for sample or amplicon cross-contamination. Examples described herein illustrate a basic, integrated approach for DNA and RNA genomes, and a simple consumable architecture for incorporating wash steps while retaining an entirely closed system. It is anticipated that integrated microarray biochemistry will provide an opportunity to significantly reduce the complexity and cost of microarray consumables, equipment, and workflow, which in turn will enable a broader spectrum of users to exploit the intrinsic multiplexing power of microarrays for infectious disease diagnostics.
Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA. dchandler@akonni.com.
Darrell P
DP
Chandler
Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA. lbryant@akonni.com.
Lexi
L
Bryant
Laboratory of Viral Diseases, Wadsworth Center, New York State Dept of Health, 120 New Scotland Avenue, Albany, NY 12208, USA. sbg03@health.state.ny.us.
Sara B
SB
Griesemer
Laboratory of Viral Diseases, Wadsworth Center, New York State Dept of Health, 120 New Scotland Avenue, Albany, NY 12208, USA. rxg11@health.state.ny.us.
Rui
R
Gu
Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA. cknickerbocker@akonni.com.
Christopher
C
Knickerbocker
Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA. akukhtin@akonni.com.
Alexander
A
Kukhtin
Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA. jparker@akonni.com.
Jennifer
J
Parker
Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA. czimmerman@akonni.com.
Cynthia
C
Zimmerman
Laboratory of Viral Diseases, Wadsworth Center, New York State Dept of Health, 120 New Scotland Avenue, Albany, NY 12208, USA. kxs16@health.state.ny.us.
Kirsten St
KS
George
Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA. cooney@akonni.com.
Christopher G
CG
Cooney
RT-PCR; asymmetric PCR; diagnostics; gel element arrays; integrated microarrays; microfluidics; multiplex; reverse transcriptase