Antitumour and Apoptotic Effects of a Plant Extract Mixture Containing Rhus verniciflua and Other Herbs in Human Leukaemia Cells.
The apoptotic effects of a novel antitumour agent (Rv-PEM01) prepared from 6 kinds of herbs, includingRhus vernicifluawere investigated using flow cytometry and western blot analysis. Rv-PEM01 induced apoptosis but not necrosis in MOLT-3, KG-1, and K562 human leukaemia cell lines. Further, Rv-PEM01-treated cells showed significantly upregulated expression of caspase-3 and 9 and cleaved caspase-3 and 9 compared to the control cells. Taken together, the results suggest that Rv-PEM01 induced apoptosis via the mitochondrial-mediated pathway, and is a potential natural anticancer agent and/or a functional food material.
International Operation Department (FM Center for R & D), Kibun Foods Inc., Tokyo, Japan.
Kohei
K
Suruga
International Operation Department (FM Center for R & D), Kibun Foods Inc., Tokyo, Japan.
Wataru
W
Hiruma
International Operation Department (FM Center for R & D), Kibun Foods Inc., Tokyo, Japan.
Kazunari
K
Kadokura
International Operation Department (FM Center for R & D), Kibun Foods Inc., Tokyo, Japan.
Tsuyoshi
T
Tomita
International Operation Department (FM Center for R & D), Kibun Foods Inc., Tokyo, Japan.
Ayaka
A
Miyata
International Operation Department (FM Center for R & D), Kibun Foods Inc., Tokyo, Japan.
Yoshihiro
Y
Sekino
Medicinal Informatics and Research Unit, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
Masahiko
M
Kimura
Ishikawa Natural Medicinal Products Research Center, Ishikawa, Japan.
Nobuo
N
Yamaguchi
Kinder-Care Veterinary Clinic, Tochigi, Japan.
Tae
T
Murai
Kitasato Institution for Life Science, Kitasato University, Tokyo, Japan.
Yasuhiro
Y
Komatsu
Medicinal Informatics and Research Unit, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
Nobufumi
N
Ono
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D008928:Mitochondria / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D029045:Rhus / Q000737:chemistry*; D015398:Signal Transduction; D015854:Up-Regulation
D000970:Antineoplastic Agents; D010936:Plant Extracts; C505407:CASP3 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053453:Caspase 9