Restriction site detection in repetitive nuclear DNA sequences of Trypanosoma evansi for strain differentiation among different isolates.
The differences or similarities among different isolates of Trypanosoma evansi through endonuclease profile was identified in the present study. The repetitive nuclear DNA of T. evansi isolated from infected cattle, buffalo and equine blood was initially amplified by PCR using specific primers. A panel of restriction enzymes, EcoRI, Eco91l, HindIII and PstI were for complete digestion of PCR products. Agarose gel electrophoresis of digested product did not show cleavage fragments and only single DNA band of the original size was visible in the ethidium bromide stained agarose gel. This indicated that the 227 bp PCR product from repetitive sequence had no site-specific cleavage sites for the REs used in this study. No heterogeneity in the repetitive nuclear DNA restriction endonuclease profile among the different isolates was recorded.
Department of Parasitology, College of Veterinary Sciences & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India.
K P
KP
Shyma
Departments of Veterinary Parasitology, College of Veterinary Science, Lala Lajapat Rai University of Veterinary and Animal Sciences, Hisar, 125004 Haryana India.
S K
SK
Gupta
Department of AGB, College of Veterinary Sciences & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India.
J P
JP
Gupta
Department of Immunology, College of Veterinary Science, Lala Lajapat Rai University of Veterinary and Animal Sciences, Hisar, 125004 Haryana India.
Ajit
A
Singh
Department of TVCC, College of Veterinary Science, Lala Lajapat Rai University of Veterinary and Animal Sciences, Hisar, 125004 Haryana India.
S S
SS
Chaudhari
Department of Parasitology, College of Veterinary Sciences & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India.
Veer
V
Singh
Buffalo; Cattle; Equine; PCR amplification; Restriction digestion; Trypanosomes