A Mutasynthetic Library of Platensimycin and Platencin Analogues.
Inactivation of ptmB1, ptmB2, ptmT2, or ptmC in Streptomyces platensis SB12029, a platensimycin (PTM) and platencin (PTN) overproducer, revealed that PTM and PTN biosynthesis features two distinct moieties that are individually constructed and convergently coupled to afford PTM and PTN. A focused library of PTM and PTN analogues was generated by mutasynthesis in the ΔptmB1 mutant S. platensis SB12032. Of the 34 aryl variants tested, 18 were incorporated with high titers.
Department of Chemistry, ‡Department of Molecular Therapeutics, and §Natural Products Library Initiative at The Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
Liao-Bin
LB
Dong
Department of Chemistry, ‡Department of Molecular Therapeutics, and §Natural Products Library Initiative at The Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
Jeffrey D
JD
Rudolf
Department of Chemistry, ‡Department of Molecular Therapeutics, and §Natural Products Library Initiative at The Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
Ben
B
Shen
D000218:Adamantane / Q000138:chemical synthesis* / Q000737:chemistry; D062365:Aminobenzoates / Q000138:chemical synthesis* / Q000737:chemistry; D000627:Aminophenols / Q000138:chemical synthesis* / Q000737:chemistry; D000813:Anilides / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D011083:Polycyclic Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D054852:Small Molecule Libraries / Q000138:chemical synthesis* / Q000737:chemistry; D013237:Stereoisomerism
D062365:Aminobenzoates; D000627:Aminophenols; D000813:Anilides; D011083:Polycyclic Compounds; D054852:Small Molecule Libraries; D000218:Adamantane; C510909:platensimycin; C520340:platencin