Molecular Characterization of Cyclamen Species Collected from Different Parts of Turkey by RAPD and SRAP Markers.
The genus Cyclamen (family Myrsinaceae) contains about 20 species, most of which occur in the Mediterranean region. Turkey has critically important Cyclamen genetic resources. Molecular characterization of plant materials collected from different regions of Turkey in which Cyclamen species grow naturally, namely Adana, Antalya, Aydın, Muğla, İzmir, Denizli, Kahramanmaraş, Osmaniye, Eskişehir, Trabzon, and Rize provinces, was performed using RAPD and SRAP markers. DNA was successfully amplified by 30 RAPD primers and 14 SRAP primer pairs. Among the 470 bands generated by the RAPD primers, 467 were polymorphic. The number of bands detected by a single primer set ranged from 11 to 22 (average of 15.6). The percentage polymorphism was 99.3 % based on the RAPD data. In the SRAP analysis, a total of 216 bands were generated, showing 100 % polymorphism. The number of bands detected by a single primer set ranged from 9 to 22 (average of 15.4). All data were scored and UPGMA dendrograms were constructed with similar results in both marker systems, i.e., different species from nine provinces of Turkey were separated from each other in the dendrograms with the same species being clustered together.
Department of Horticulture, Faculty of Agriculture, University of Çukurova, 01330, Adana, Turkey.
Ozhan
O
Simsek
Department of Horticulture, Faculty of Agriculture, University of Çukurova, 01330, Adana, Turkey.
Pembe
P
Curuk
Institute of Natural and Applied Sciences, Department of Biotechnology, University of Çukurova, 01330, Adana, Turkey.
Fatma
F
Aslan
Department of Horticulture, Faculty of Agriculture, University of Çukurova, 01330, Adana, Turkey.
Melda
M
Bayramoglu
Department of Horticulture, Faculty of Agriculture, University of Ege, 35040, İzmir, Turkey.
Tolga
T
Izgu
Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Ikenobe, Miki-cho, 761-0795, Japan.
Jaime A Teixeira
JA
da Silva
Department of Horticulture, Faculty of Agriculture, University of Çukurova, 01330, Adana, Turkey. ykacar@cu.edu.tr.
Yildiz Aka
YA
Kacar
Department of Horticulture, Faculty of Agriculture, University of Çukurova, 01330, Adana, Turkey.
Yesim Yalcin
YY
Mendi
D031824:Cyclamen / Q000145:classification / Q000235:genetics*; D005819:Genetic Markers; D005838:Genotype; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D014421:Turkey
D005819:Genetic Markers
DNA; Genetic relationships; Genetic resources; PCR; Polymorphism