Detection of norovirus virus-like particles using a surface plasmon resonance-assisted fluoroimmunosensor optimized for quantum dot fluorescent labels.
A highly sensitive biosensor to detect norovirus in environment is desired to prevent the spread of infection. In this study, we investigated a design of surface plasmon resonance (SPR)-assisted fluoroimmunosensor to increase its sensitivity and performed detection of norovirus virus-like particles (VLPs). A quantum dot fluorescent dye was employed because of its large Stokes shift. The sensor design was optimized for the CdSe-ZnS-based quantum dots. The optimal design was applied to a simple SPR-assisted fluoroimmunosensor that uses a sensor chip equipped with a V-shaped trench. Excitation efficiency of the quantum dots, degree of electric field enhancement by SPR, and intensity of autofluorescence of a substrate of the sensor chip were theoretically and experimentally evaluated to maximize the signal-to-noise ratio. As the result, an excitation wavelength of 390nm was selected to excite SPR on an Al film of the sensor chip. The sandwich assay of norovirus VLPs was performed using the designed sensor. Minimum detectable concentration of 0.01ng/mL, which corresponds to 100 virus-like particles included in the detection region of the V-trench, was demonstrated.
Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan. Electronic address: h.ashiba@aist.go.jp.
Hiroki
H
Ashiba
Department of Electrical Engineering and Bioscience, Waseda University, Shinjuku, Japan.
Yuki
Y
Sugiyama
Nanoelectronics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Xiaomin
X
Wang
Department of Virology II, National Institute of Infectious Diseases, Musashi-Murayama, Japan.
Haruko
H
Shirato
Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Japan.
Kyoko
K
Higo-Moriguchi
Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Japan.
Koki
K
Taniguchi
Department of Electrical Engineering and Bioscience, Waseda University, Shinjuku, Japan.
Yoshimichi
Y
Ohki
Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Makoto
M
Fujimaki
D015374:Biosensing Techniques; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000821:virology*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D029322:Norovirus / Q000302:isolation & purification* / Q000472:pathogenicity; D045663:Quantum Dots; D059629:Signal-To-Noise Ratio; D020349:Surface Plasmon Resonance
D005456:Fluorescent Dyes
Norovirus detection; Quantum dot; Sensitivity enhancement; Sensor design; Surface plasmon resonance; V-trench biosensor