The Comparative Analysis of Antioxidant and Biological Activity for the Dendropanax morbifera LEV. Leaves Extracted by Different Ethanol Concentrations.
The different concentrations of ethanol (20-100%) and distilled water extract for Dendropanax morbifera LEV. leaves were evaluated to induce antioxidant and biological activity employed by variety of assays. The 20%, 80%, and 100% ethanol extract demonstrated the relatively higher activity, whereas distilled water, 40%, and 60% ethanol extracts exhibited the lower antioxidant and biological activity. Especially, 80% ethanol extract showed the remarkably higher radical scavenging activity, reducing power, total phenol and flavonoid content, α-glucosidase, and tyrosinase inhibitory activity, and alcohol dehydrogenases (ADH) and aldehyde dehydrogenase (ALDH) activity. Also, 100% ethanol extract exhibited relatively greater activity, but there did not show significant radical scavenging activity. Furthermore, there were 50% and 30% promotion effect for ADH activity assay and 80% and 40% promotion effect for ALDH activity assay in 80% and 100% ethanol extract, respectively. In addition, in the minimum inhibitory concentration (MIC), all extracts except for distilled water extract inhibited Bacillus cereus, Staphylococcus aureus subsp. aureus, Escherichia coli. For Pichia jadinii, whole extracts effectively inhibited yeast multiplication at concentration of 125 μg/mL for 100% ethanol extract and 250 μg/mL for the rest of extract. These result indicated that D. morbifera LEV. leaves extracted by 80% ethanol would be the ideal extracting solution to maximize inherent antioxidant and biological activity agent.
Majors in Plant Resource and Environment, College of Agriculture & Life Science, SARI, Jeju National University.
Masaya
M
Nakamura
Jong-Hwan
JH
Ra
Ju-Sung
JS
Kim
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D027481:Araliaceae / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol; D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers; D065089:Glycoside Hydrolase Inhibitors; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis; D014867:Water
D000890:Anti-Infective Agents; D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D059808:Polyphenols; D014867:Water; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D014442:Monophenol Monooxygenase; D000444:Aldehyde Dehydrogenase