Mapping quantitative trait loci responsible for resistance to Bakanae disease in rice.
BACKGROUND
Bakanae or foot rot disease caused by Fusarium fujikuroi [teleomorph: Gibberella fujikuroi (Sawada) Ito] is emerging as a serious disease in rice. The disease causes both quantitative and qualitative losses to the grains under the field conditions. Breeding for resistance to Bakanae disease is a promising strategy to manage this emerging disease. In this study, we used a population of 168 F14 recombinant inbred lines (RILs) derived from two indica rice parents Pusa 1342, a highly resistant variety and Pusa Basmati 1121, a highly susceptible variety to map quantitative trait loci (QTLs) governing resistance against Bakanae disease.


RESULTS
The disease reaction of 168 F14 RILs were measured on the seedlings inoculated using Fusarium fujikuroi culture using high-throughput screening protocol under glasshouse conditions. Utilizing inclusive composite interval mapping, three QTLs governing resistance to Bakanae were identified, namely qBK1.1, qBK1.2 and qBK1.3 which accounted 4.76, 24.74 and 6.49 % of phenotypic variation, respectively. The major effect QTL designated qBK1.2 was mapped in 0.26 Mb region between RM5336 and RM10153. A total of 55 annotated genes were identified within the identified QTL region qBK1.2.


CONCLUSIONS
The novel QTLs identified in this study are useful resource for efficiently breeding rice cultivars resistant to Bakanae disease. This is the first report on identification of QTLs governing resistance against Bakanae in rice using inclusive composite interval mapping strategy in a RIL population.
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.|Present address: ICAR-Indian Institute of Rice Research, Hyderabad, 500030, India.
R Abdul
RA
Fiyaz
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Ashutosh K
AK
Yadav
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
S Gopala
SG
Krishnan
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Ranjith K
RK
Ellur
Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Bishnu M
BM
Bashyal
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Nitasha
N
Grover
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Prolay K
PK
Bhowmick
Rice Breeding and Genetics Research Centre, ICAR-Indian Agricultural Research Institute, Aduthurai, 612101, India.
M
M
Nagarajan
Rice Breeding and Genetics Research Centre, ICAR-Indian Agricultural Research Institute, Aduthurai, 612101, India.
K K
KK
Vinod
ICAR-National Research Centre on Plant Biotechnology, New Delhi, 110012, India.
Nagendra K
NK
Singh
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Kumble V
KV
Prabhu
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India. aks_gene@yahoo.com.
Ashok K
AK
Singh
Bakanae; Foot rot; Fusarium fujikuroi; QTL mapping; Resistance; Rice