Facile Preparation of Multifunctional WS2/WOxNanodots for Chelator-Free89Zr-Labeling and In Vivo PET Imaging.
While position emission tomography (PET) is an important molecular imaging technique for both preclinical research and clinical disease diagnosis/prognosis, chelator-free radiolabeling has emerged as a promising alternative approach to label biomolecules or nanoprobes in a facile way. Herein, starting from bottom-up synthesized WS2nanoflakes, this study fabricates a unique type of WS2/WOxnanodots, which can function as inherent hard oxygen donor for stable radiolabeling with Zirconium-89 isotope (89Zr). Upon simply mixing,89Zr can be anchored on the surface of polyethylene glycol (PEG) modified WS2/WOx(WS2/WOx-PEG) nanodots via a chelator-free method with surprisingly high labeling yield and great stability. A higher degree of oxidation in the WS2/WOx-PEG sample (WS2/WOx(0.4)) produces more electron pairs, which would be beneficial for chelator-free labeling of89Zr with higher yields, suggesting the importance of surface chemistry and particle composition to the efficiency of chelator-free radiolabeling. Such89Zr-WS2/WOx(0.4)-PEG nanodots are found to be an excellent PET contrast agent for in vivo imaging of tumors upon intravenous administration, or mapping of draining lymph nodes after local injection.
Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu, 215123, China.|Department of Radiology, University of Wisconsin-Madison, WI, 53705, USA.
Liang
L
Cheng
Department of Radiology, University of Wisconsin-Madison, WI, 53705, USA.
Anyanee
A
Kamkaew
Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu, 215123, China.
Sida
S
Shen
Department of Medical Physics, University of Wisconsin-Madison, WI, 53705, USA.
Hector F
HF
Valdovinos
Department of Radiology, University of Wisconsin-Madison, WI, 53705, USA.
Haiyan
H
Sun
Department of Radiology, University of Wisconsin-Madison, WI, 53705, USA.
Reinier
R
Hernandez
Materials Science Program, University of Wisconsin-Madison, WI, 53705, USA.
Shreya
S
Goel
Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu, 215123, China.
Teng
T
Liu
Department of Radiology, University of Wisconsin-Madison, WI, 53705, USA.
Cyrus R
CR
Thompson
Department of Medical Physics, University of Wisconsin-Madison, WI, 53705, USA.
Todd E
TE
Barnhart
Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu, 215123, China. zliu@suda.edu.cn.
Zhuang
Z
Liu
Department of Radiology, University of Wisconsin-Madison, WI, 53705, USA. wcai@uwhealth.org.|Department of Medical Physics, University of Wisconsin-Madison, WI, 53705, USA. wcai@uwhealth.org.|Materials Science Program, University of Wisconsin-Madison, WI, 53705, USA. wcai@uwhealth.org.|University of Wisconsin Carbone Cancer Center, Madison, WI, 53705, USA. wcai@uwhealth.org.
Weibo
W
Cai
D000818:Animals; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000737:chemistry*; D005260:Female; D008198:Lymph Nodes / Q000473:pathology; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry*; D049268:Positron-Emission Tomography / Q000379:methods*; D011868:Radioisotopes / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
D002614:Chelating Agents; D010087:Oxides; D011868:Radioisotopes; D013440:Sulfides; D015040:Zirconium; C000615502:Zirconium-89
89Zr-chelator-free; WS2/WOx nanodots; in vivo PET imaging; lymph node imaging; stability