Hemoglobin-Conjugated Gelatin Microsphere as a Smart Oxygen Releasing Biomaterial.
In this study, a novel micrometric biomaterial acting as a cyclic oxygen releasing system is designed. Human hemoglobin (Hb) is conjugated to the surface of gelatin microspheres (GM) to produce gelatin hemoglobin oxygen depot (G-HbOD). G-HbOD is obtained by means of two different conjugation strategies. The degree of conjugation of GM surfaces in terms of free amino groups by using HPLC is first evaluated. By following the strategy A (G-HbOD_A), Hb is conjugated to GM by means of the formation of a polyurethane linker. In the strategy B (G-HbOD_B) the conjugation occurs via amide bound formation. Physical and morphological differences between G-HbOD_A and G-HbOD_B are investigated by means of Fourier Transform Infrared Spectroscopy (FTIR), Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES), Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Differences in oxygen uptake/release kinetics are found depending on the conjugation strategy and it is proved that G-HbOD works under repeated cycles in microfluidic chip. Moreover, G-HbOD is also able to work as oxygen depot in the early stages of 3D cell cultures.
Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci n. 53, 80125, Napoli, Italy.|Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, P.le Tecchio 80, 80125, Napoli, Italy.
Antonio
A
Paciello
Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci n. 53, 80125, Napoli, Italy.|Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, P.le Tecchio 80, 80125, Napoli, Italy.
Giuseppe
G
Amalfitano
Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci n. 53, 80125, Napoli, Italy.|Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, P.le Tecchio 80, 80125, Napoli, Italy.
Alessandro
A
Garziano
Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci n. 53, 80125, Napoli, Italy. francesco.urciuolo@iit.it.
Francesco
F
Urciuolo
Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci n. 53, 80125, Napoli, Italy.|Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, P.le Tecchio 80, 80125, Napoli, Italy.|Department of Chemical, Materials and Industrial Production Engineering (DICMAPI), University of Napoli Federico II, P.le Tecchio 80, 80125, Napoli, Italy.
Paolo A
PA
Netti
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D005780:Gelatin / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008863:Microspheres; D041681:NIH 3T3 Cells; D010100:Oxygen / Q000737:chemistry*; D010316:Particle Size; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
D001672:Biocompatible Materials; D004337:Drug Carriers; D006454:Hemoglobins; D005780:Gelatin; C020622:hemoglobin B; D010100:Oxygen
3D cell culture; amino group; gelatin; hemoglobin conjugation; hemoglobin oxygen carriers; oxygen release