Smart bomb AS1411 aptamer-functionalized/PAMAM dendrimer nanocarriers for targeted drug delivery in the treatment of gastric cancer.
Chemotherapy, a conventional method assessed in recent oncology studies, poses numerous problems in the clinical environment. To overcome the problems inherent in chemotherapy, an intelligent drug delivery system has come to the forefront of cancer therapeutics. In this study, we designed a dendrimer-based pharmaceutical system together with a single-stranded AS1411 aptamer (APTAS1411) as a therapeutic strategy. The polyamidoamine (PAMAM)-polyethylene glycol (PEG) complex was then conjugated with the AS1411 aptamer and confirmed by atomic-force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) .In this study, we show that the conjugated PAMAM-PEG-APTAS1411complex dramatically increased PAMAM-PEG-5-FU uptake by MKN45 gastric cancer cells. We also demonstrated both the stability of the nanoparticle-5-FU-APTAS1411complex, by thin layer chromatography (TLC), and an increase in 5-fluorouracil (5-FU) accumulation in the vicinity of cancerous tumors. This smart drug delivery system is capable of effectively transferring 5-FU to MKN45 gastric cancer cells in consistent and without toxic effects.
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Amir
A
Barzegar Behrooz
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Fatemeh
F
Nabavizadeh
Biomedical Engineering and Medical physics, Department of Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Jamal
J
Adiban
Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Mehdi
M
Shafiee Ardestani
Medical Lab Technology Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Rouhollah
R
Vahabpour
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
Mohammad Reza
MR
Aghasadeghi
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Hamid
H
Sohanaki
D000818:Animals; D052157:Aptamers, Nucleotide / Q000008:administration & dosage* / Q000378:metabolism; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000008:administration & dosage* / Q000378:metabolism; D004337:Drug Carriers / Q000008:administration & dosage / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D016896:Treatment Outcome; D023041:Xenograft Model Antitumor Assays / Q000379:methods
C513936:AGRO 100; D052157:Aptamers, Nucleotide; D050091:Dendrimers; D004337:Drug Carriers; D009838:Oligodeoxyribonucleotides; C104700:PAMAM Starburst
5-fluorouracil; PAMAM; aptamer; drug delivery; nucleolin