Vitamin E-Labeled Polyethylenimine for in vitro and in vivo Gene Delivery.
A series of Vitamin E (vitE)-labeled PEIs (PEI-vitEn) were synthesized and showed excellent complexation ability with plasmid DNA (pDNA). The cellular uptake of PEI-vitEn/pDNA complexes was greatly enhanced with the increase of vitE labeling, which is much better than that of control PEI25 in three different cell lines. PEI-vitE6showed the best performance in gfp pDNA delivery and following GFP expression in HEK-293A cells. In addition, in vivo gene delivery in living mice also confirmed that PEI-vitE6showed low toxicity and efficiently delivered gfp pDNA to the cells of liver and lung tissues for gene expression.
State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, School of Pharmaceutical Sciences, Peking University , No. 38 Xueyuan Road Beijing, 100191, China.
Jinxing
J
Liu
State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, School of Pharmaceutical Sciences, Peking University , No. 38 Xueyuan Road Beijing, 100191, China.
Mengke
M
Feng
State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, School of Pharmaceutical Sciences, Peking University , No. 38 Xueyuan Road Beijing, 100191, China.
Duanwei
D
Liang
State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, School of Pharmaceutical Sciences, Peking University , No. 38 Xueyuan Road Beijing, 100191, China.
Jiali
J
Yang
State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, School of Pharmaceutical Sciences, Peking University , No. 38 Xueyuan Road Beijing, 100191, China.
Xinjing
X
Tang
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D010957:Plasmids / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D011094:Polyethyleneimine / Q000008:administration & dosage / Q000737:chemistry*; D014810:Vitamin E / Q000008:administration & dosage / Q000737:chemistry* / Q000235:genetics
D014810:Vitamin E; D011094:Polyethyleneimine; D004247:DNA