CRISPR/Cas9-Mediated Genome Editing and Mutagenesis ofEcChi4inExopalaemon carinicauda.
The development of the type II clustered regularly interspaced short palindromic repeats (CRISPR) system has resulted in the revolution of genetic engineering, and this technology has been applied in the genome editing of various species. However, there are no reports about target-specific genome editing in shrimp. In this research, we developed a microinjection method for the ridgetail white prawnExopalaemon carinicaudaand successfully applied CRISPR/Cas9 technology to the genome editing ofE. carinicaudaThrough coinjection of mRNA of Cas9 nuclease and gRNA specialized forE. carinicaudachitinase 4 (EcChi4), shrimps with indel mutations were obtained. Further analysis showed that the mutations could be transmitted to the next generation. This is the first time that site-specific genome editing has been successfully demonstrated in a decapod, and will further contribute to the study of functional genomics in decapods.
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Tianshu
T
Gui
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China zhangjiquan@qdio.ac.cn.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China.
Jiquan
http://orcid.org/0000-0001-5063-339X
J
Zhang
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Fengge
F
Song
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.|College of Marine Life and Fisheries, Huaihai Institute of Technology, Lianyungang 222005, China.
Yuying
Y
Sun
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Shijun
S
Xie
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Kuijie
K
Yu
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China.
Jianhai
J
Xiang
CRISPR/Cas9; Exopalaemon carinicauda; chitinase; microinjection