Exogenous Ghrelin Accelerates the Healing of Acetic Acid-Induced Colitis in Rats.
Previous studies have shown that ghrelin reduces colonic inflammation induced by trinitrobenzene sulfonic acid and dextran sodium sulfate. In the present study we determined the effect of treatment with ghrelin on the course of acetic acid-induced colitis in rats. Rectal administration of 3% acetic acid solution led to induction of colitis in all animals. Damage of the colonic wall was accompanied by an increase in mucosal concentration of pro-inflammatory interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), as well mucosal activity of myeloperoxidase. Moreover, induction of colitis led to a reduction in colonic blood flow and DNA synthesis. Administration of ghrelin after induction of colitis led to faster regeneration of the colonic wall and reduction in colonic levels of IL-1β, TNF-α, and myeloperoxidase. In addition, treatment with ghrelin improved mucosal DNA synthesis and blood flow. Our study disclosed that ghrelin exhibits a strong anti-inflammatory and healing effect in acetic acid-induced colitis. Our current observation in association with previous findings that ghrelin exhibits curative effect in trinitrobenzene sulfonic acid- and dextran sodium sulfate-induced colitis suggest that therapeutic effect of ghrelin in the colon is universal and independent of the primary cause of colitis.
Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka St., 31-531 Cracow, Poland. aleksandra.matuszyk@uj.edu.pl.|Department of Anatomy, Faculty of Medicine, Jagiellonian University Medical College, 31-034 Cracow, Poland. aleksandra.matuszyk@uj.edu.pl.
Aleksandra
A
Matuszyk
Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka St., 31-531 Cracow, Poland. piotr.ceranowicz@uj.edu.pl.
Piotr
P
Ceranowicz
Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka St., 31-531 Cracow, Poland. mpwarzec@cyf-kr.edu.pl.
Zygmunt
Z
Warzecha
Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka St., 31-531 Cracow, Poland. jakub.cieszkowski@uj.edu.pl.
Jakub
J
Cieszkowski
Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka St., 31-531 Cracow, Poland. dagac@op.pl.|Department of Pediatrics, Gastroenterology and Nutrition, University Children's Hospital, Faculty of Medicine, Jagiellonian University Medical College, 30-663 Cracow, Poland. dagac@op.pl.
Dagmara
D
Ceranowicz
Department of Pathomorphology, Faculty of Medicine, Jagiellonian University Medical College, 31-531 Cracow, Poland. krystyna.galazka@uj.edu.pl.
Krystyna
K
Gałązka
Department of Medical Physiology Faculty of Health Sciences, Jagiellonian University Medical College, 31-126 Cracow, Poland. joanna.bonior@uj.edu.pl.
Joanna
J
Bonior
Department of Medical Physiology Faculty of Health Sciences, Jagiellonian University Medical College, 31-126 Cracow, Poland. jolanta.jaworek@uj.edu.pl.
Jolanta
J
Jaworek
Department of Cardiovascular Surgery and Transplantology, Jagiellonian University, JP II Hospital, 31-202 Cracow, Poland. krzysztof.bartus@uj.edu.pl.
Krzysztof
K
Bartuś
Department of Pathophysiology, Faculty of Medicine, Jagiellonian University Medical College, 31-121 Cracow, Poland. krzysztof.m.gil@uj.edu.pl.
Krzysztof
K
Gil
Department of Pharmacology, Faculty of Medicine, Jagiellonian University Medical College, 31-531 Cracow, Poland. rafal.olszanecki@uj.edu.pl.
Rafał
R
Olszanecki
Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka St., 31-531 Cracow, Poland. mpdembin@cyf-kr.edu.pl.
Artur
A
Dembiński
D019342:Acetic Acid / Q000633:toxicity; D000818:Animals; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000209:etiology; D004247:DNA / Q000096:biosynthesis; D054439:Ghrelin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D054439:Ghrelin; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D004247:DNA; D009195:Peroxidase; D019342:Acetic Acid
DNA synthesis; colitis; interleukin-1β; mucosal blood flow; myeloperoxidase