Cytotoxicity assessment of adipose-derived mesenchymal stem cells on synthesized biodegradable Mg-Zn-Ca alloys.
Magnesium (Mg)-based alloys have been extensively considered as biodegradable implant materials for orthopedic surgery. Mg and its alloys are metallic biomaterials that can degrade in the body and promote new bone formation. In this study, the corrosion behavior and cytotoxicity of Mg-Zn-Ca alloys are evaluated with adipose-derived mesenchymal stem cells (ASCs). Mg-2Zn and Mg-2Zn-xCa (x=1, 2 and 3wt.%) alloys were designated. Mg alloys were analyzed with scanning electron microscopy and potentiodynamic polarization. To understand the in-vitro biocompatibility and cytotoxicity of Mg-2Zn and Mg-2Zn-xCa alloys, ASCs were cultured for 24 and 72h in contact with 10%, 50% and 100% extraction of all alloys prepared in DMEM. Cell cytotoxicity and viability of ASCs were examined by MTT assay. Alloying elements including Zn and Ca improved the corrosion resistance of alloys were compared with pure Mg. The cytotoxicity results showed that all alloys had no significant adverse effects on cell viability in 24h. After 72h, cell viability and proliferation increased in the cells exposed to pure Mg and Mg-2Zn-1Ca extracts. The release of Mg, Zn and Ca ions in culture media had no toxic impacts on ASCs viability and proliferation. Mg-2Zn-1Ca alloy can be suggested as a good candidate to be used in biomedical applications.
Department of Biomedical Engineering, Materials and Biomaterials Research Center (MBMRC), Tehran, IR, Iran.
Abbas
A
Fazel Anvari-Yazdi
Endometriosis and Gynecologic Disorders Research Center, Department of Ob. & Gyn., Rasoul-e Akram Hospital, Iran University of Medical Sciences (IUMS), Tehran, IR, Iran. Electronic address: tahermanesh.k@iums.ac.ir.
Kobra
K
Tahermanesh
Materials and Energy Research Center (MERC), Karaj, IR, Iran.
Seyed Mohammad Mehdi
SM
Hadavi
Tissue Engineering Lab, Anatomy Department, School of Medicine, Shiraz University of Medical Sciences (SUMS), Shiraz, IR, Iran.
Tahereh
T
Talaei-Khozani
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences (SUMS), Shiraz, IR, Iran.
Mahboobeh
M
Razmkhah
School of Medicine, Yasuj University of Medical Sciences (YUMS), Yasuj, IR, Iran.
Seyedeh Mehr
SM
Abed
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences (SUMS), Shiraz, IR, Iran.
Maryam Sadat
MS
Mohtasebi
D000273:Adipose Tissue / Q000166:cytology*; D000497:Alloys / Q000494:pharmacology*; D015703:Antigens, CD / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003343:Corrosion; D004563:Electrochemistry; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D008970:Molecular Weight; D009994:Osmolar Concentration; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D015032:Zinc / Q000494:pharmacology*
D000497:Alloys; D015703:Antigens, CD; D001672:Biocompatible Materials; D008274:Magnesium; D015032:Zinc; D002118:Calcium
Adipose stem cells; Biodegradation; Bone tissue; Corrosion; Cytotoxicity; Magnesium alloy