Investigation of the pH-dependence of dye-doped protein-protein interactions.
Proteins can dramatically change their conformation under environmental conditions such as temperature and pH. In this context, Glycoprotein's conformational determination is challenging. This is due to the variety of domains which contain rich chemical characters existing within this complex. Here we demonstrate a new, straightforward and efficient technique that uses the pH-dependent properties of dyes-doped Pig Gastric Mucin (PGM) for predicting and controlling protein-protein interaction and conformation. We utilize the PGM as natural host matrix which is capable of dynamically changing its conformational shape and adsorbing hydrophobic and hydrophilic dyes under different pH conditions and investigate and control the fluorescent properties of these composites in solution. It is shown at various pH conditions, a large variety of light emission from these complexes such as red, green and white is obtained. This phenomenon is explained by pH-dependent protein folding and protein-protein interactions that induce different emission spectra which are mediated and controlled by means of dye-dye interactions and surrounding environment. This process is used to form the technologically challenging white light-emitting liquid or solid coating for LED devices.
School of Chemistry Faculty of Exact Sciences, Tel- Aviv University, Tel-Aviv, Israel.|Center for Nanoscience and nanotechnology, Tel- Aviv University, Tel-Aviv, Israel.
Roman
R
Nudelman
School of Chemistry Faculty of Exact Sciences, Tel- Aviv University, Tel-Aviv, Israel.|Center for Nanoscience and nanotechnology, Tel- Aviv University, Tel-Aviv, Israel.
Ekaterina
E
Gloukhikh
School of Chemistry Faculty of Exact Sciences, Tel- Aviv University, Tel-Aviv, Israel.
Antonina
A
Rekun
Center for Nanoscience and nanotechnology, Tel- Aviv University, Tel-Aviv, Israel. srichter@post.tau.ac.il.|The Department of Materials Science and Engineering, Faculty of Engineering, Tel- Aviv University, Tel-Aviv, Israel. srichter@post.tau.ac.il.
Shachar
S
Richter
D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry*; D005752:Gastric Mucins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D017510:Protein Folding; D013552:Swine
D005456:Fluorescent Dyes; D005752:Gastric Mucins
hydrophobic and hydrophilic interactions; mucins; protein folding; white LED