Lonicera caerulea berry extract suppresses lipopolysaccharide-induced inflammation via Toll-like receptor and oxidative stress-associated mitogen-activated protein kinase signaling.
The protective effects of Lonicera caerulea berry extract (LCBE) against hepatic inflammation and the underlying mechanisms were investigated in a rat model of lipopolysaccharide (LPS)-induced chronic liver inflammation. Male Sprague-Dawley rats were injected with LPS (200 μg per kg bw) with or without LCBE co-administration (50, 100 and 200 mg per kg bw intragastrically once daily) for 4 weeks. We found that LCBE supplementation inhibited the increase in Toll-like receptor (TLR)2 and TLR4 expression induced by LPS, while preventing glutathione depletion and reactive oxidative species generation and abrogating increases in C-reactive protein and interleukin-6 levels, restoring alanine and aspartate aminotransferase activities, and blocking the phosphorylation of p38 and c-Jun N-terminal kinase mitogen-activated protein kinases (MAPKs). The protective effects of LCBE against liver damage caused by LPS were dose-dependent. These results demonstrate that LCBE suppresses liver inflammation caused by LPS via inhibition of TLR and MAPK signaling and oxidative stress pathways, and suggest that LCBE treatment can potentially prevent chronic liver injury.
Shenyang Agricultural University, Shenyang, Liaoning 110866, China. mengxjsy@126.com.
Yuehua
Y
Wang
Shenyang Agricultural University, Shenyang, Liaoning 110866, China. mengxjsy@126.com.
Bin
B
Li
Shenyang Agricultural University, Shenyang, Liaoning 110866, China. mengxjsy@126.com and Food Inspection Monitoring Center of Zhuanghe, Dalian, Liaoning 116400, China.
Jinyan
J
Zhu
Shenyang Agricultural University, Shenyang, Liaoning 110866, China. mengxjsy@126.com.
Qi
Q
Zhang
Shenyang Agricultural University, Shenyang, Liaoning 110866, China. mengxjsy@126.com.
Xiuyan
X
Zhang
Shenyang Agricultural University, Shenyang, Liaoning 110866, China. mengxjsy@126.com.
Li
L
Li
Shenyang Normal University, Shenyang, Liaoning 110034, China. ma1976@126.com.
Yan
Y
Ma
Shenyang Agricultural University, Shenyang, Liaoning 110866, China. mengxjsy@126.com.
Xianjun
X
Meng
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry*; D007249:Inflammation / Q000139:chemically induced* / Q000188:drug therapy; D008070:Lipopolysaccharides / Q000633:toxicity*; D031239:Lonicera / Q000737:chemistry*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D051193:Toll-Like Receptors / Q000378:metabolism*
D008070:Lipopolysaccharides; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D051193:Toll-Like Receptors