Cytokinin membrane receptors participate in regulation of plastid genome expression in the skotomorphogenesis.
Analysis by real-time PCR of single and double insertion mutants of A. thaliana with inactivated cytokinin receptor genes showed that the level of transcripts of some of plastid genes during skotomorphogenesis depended on the state of functionally active receptor AHK3. The cytokinin-regulated plastid encoded genes involved the housekeeping genes (rpoB and accD) and the genes for photosynthetic proteins (ndhA, psbA, atpB, and psaA). However, the absence of hormone activation of plastid encoded genes engaged in the translation of plastid proteins, rRNA (rrn16), and tRNA (trnE), indirectly indicate the disruption of the synthesis of chloroplast proteins in the dark.
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, Moscow, 127276, Russia.|Russian State Agrarian University-Timiryazev Agricultural Academy, ul. Timiryazeva 47, Moscow, 127550, Russia.
A S
AS
Doroshenko
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, Moscow, 127276, Russia. mariadanilova86@yandex.ru.
M N
MN
Danilova
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, Moscow, 127276, Russia.
N V
NV
Kudryakova
Russian State Agrarian University-Timiryazev Agricultural Academy, ul. Timiryazeva 47, Moscow, 127550, Russia.
A A
AA
Soloviev
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, Moscow, 127276, Russia.
V V
VV
Kusnetsov
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003583:Cytokinins / Q000378:metabolism; D003624:Darkness; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D055786:Gene Knockout Techniques; D054627:Genome, Plastid / Q000502:physiology; D000071677:Histidine Kinase / Q000235:genetics / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
C549195:ARR5 protein, Arabidopsis; D029681:Arabidopsis Proteins; D003583:Cytokinins; D011956:Receptors, Cell Surface; D014157:Transcription Factors; D011494:Protein Kinases; D000071677:Histidine Kinase; C000605492:AHK2 protein, Arabidopsis; C000605493:AHK3 protein, Arabidopsis; C421783:WOL protein, Arabidopsis