Decreased expression of GLUT4 in male CG-IUGR rats may play a vital role in their increased susceptibility to diabetes mellitus in adulthood.
Rats with intrauterine growth retardation and catch-up growth (CG-IUGR) after birth show increased susceptibility to diabetes mellitus in adulthood. The expression of glucose transporter type 4 (GLUT4) decreases in female IUGR offspring rats with seminutrient restriction during pregnancy. However, the male CG-IUGR rats also display an increased susceptibility to diabetes mellitus in adulthood. Whether there is another factor, besides GLUT4, in male CG-IUGR rat that mediates their susceptibility to diabetes mellitus? The male IUGR rats with catch-up growth were selected as the research objects. CG-IUGR rats had an increased fasting blood glucose level, and increased serum total cholesterol, triglyceride and free fatty acid levels. Glucose tolerance test and insulin tolerance test showed higher glucose levels and much higher insulin levels after a glucose load in CG-IUGR. The mRNA and protein expressions of IRS-2 in liver tissue, and IRS-1 and GLUT4 in skeletal muscle in CG-IUGR rats were down-regulated, but only the GLUT4 down-regulation displayed strong negative correlations with the decreased glucose tolerance capability by Pearson's analysis. The methylation patterns of CpG islands in the promoter regions of IRS-1, IRS-2 and GLUT4 in CG-IUGR rats varied, which was not significantly correlated with their expressions. The male CG-IUGR rats showed decreased glucose tolerant capability, suggesting increased susceptibility to diabetes mellitus in adulthood. The GLUT4 down-regulation may play a vital role in the development of decreased glucose tolerance in male CG-IUGR rats. The methylation modification of the promoter region of GLUT4 does not appear to be involved in its expression.
Department of Pathophysiology, Zunyi Medical University, Zunyi 563003, China.
Chang
C
Duan
Stomatological Hospital Affiliated to Zunyi Medical University, Zunyi 563003, China.
Min
M
Liu
Department of Pathophysiology, Zunyi Medical University, Zunyi 563003, China.
Haiyan
H
Xu
Department of Pathophysiology, Zunyi Medical University, Zunyi 563003, China.
Weiwei
W
Tang
Department of Pathophysiology, Zunyi Medical University, Zunyi 563003, China.
Jiayun
J
Liu
Department of Pathophysiology, Zunyi Medical University, Zunyi 563003, China.
Lamei
L
Hou
Department of Pathophysiology, Zunyi Medical University, Zunyi 563003, China linda_master@eyou.com.
Lijuan
L
Li
D000367:Age Factors; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D001835:Body Weight / Q000235:genetics*; D019175:DNA Methylation; D003920:Diabetes Mellitus / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D005317:Fetal Growth Retardation / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005951:Glucose Tolerance Test; D051275:Glucose Transporter Type 4 / Q000235:genetics* / Q000378:metabolism; D007328:Insulin / Q000097:blood; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012737:Sex Factors
D001786:Blood Glucose; D051275:Glucose Transporter Type 4; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; C495694:Slc2a4 protein, rat
CG-IUGR; DNA methylation; GLUT4; diabetes mellitus; metabolism