CO2Biofixation of Actinobacillus succinogenes Through Novel Amine-Functionalized Polystyrene Microsphere Materials.
CO2-derived succinate production was enhanced by Actinobacillus succinogenes through polystyrene (PSt) microsphere materials for CO2adsorption in bioreactor, and the adhesion forces between A. succinogenes bacteria and PSt materials were characterized. Synthesized uniformly sized and highly cross-linked PSt microspheres had high specific surface areas. After modification with amine functional groups, the novel amine-functionalized PSt microspheres exhibited a high adsorption capacity of 25.3 mg CO2/g materials. After addition with the functionalized microspheres into the culture broth, CO2supply to the cells increased. Succinate production by A. succinogenes can be enhanced from 29.6 to 48.1 g L-1. Moreover, the characterization of interaction forces between A. succinogenes cells and the microspheres indicated that the maximal adhesive force was about 250 pN. The amine-functionalized PSt microspheres can adsorb a large amount of CO2and be employed for A. succinogenes anaerobic cultivation in bioreactor for high-efficiency production of CO2-derived succinate.
Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
Wenhao
W
Zhu
University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China. qiangli@ipe.ac.cn.|National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China. qiangli@ipe.ac.cn.
Qiang
Q
Li
Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, People's Republic of China. 71341636@qq.com.
Ning
N
Dai
D065966:Absorption, Physicochemical; D000188:Actinobacillus / Q000378:metabolism*; D000393:Air Pollutants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000588:Amines / Q000097:blood*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D002245:Carbon Dioxide / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008863:Microspheres; D011137:Polystyrenes / Q000097:blood*; D019802:Succinic Acid / Q000302:isolation & purification / Q000378:metabolism*
D000393:Air Pollutants; D000588:Amines; D011137:Polystyrenes; D002245:Carbon Dioxide; D019802:Succinic Acid
Actinobacillus succinogenes; Amine-functionalized polystyrene microsphere; CO2 sequestration; CO2-derived succinate