Effects of Shenfu Injection () on cerebral metabolism in a porcine model of cardiac arrest.
OBJECTIVE
To investigate the effects of Shenfu Injection (, SFI) on cerebral metabolism in a porcine model of cardiac arrest (CA).


METHODS
Thirty Wuzhishan minipigs were randomly assigned to the control group (n=6), epinephrine group (EP group, n=12) and Sfigroup (n=12). After 8 min of untreated ventricular fifibrillation (VF), pigs in the EP group or Sfigroup were administered with either EP (0.02 mg/kg) or Sfi(1.0 mL/kg), respectively. After successful resuscitation, cerebrospinal fluid (CSF) levels of glucose, pyruvate, lactate, glutamate and glycerol were measured at 1, 6, 12 and 24 h after recover from spontaneous circulation (ROSC). In addition, neurologic defificit score (NDS) was calculated at 24 h after ROSC. Surviving pigs were killed at 24 h after ROSC, and the brain tissue was obtained for ultra-microstructure examination.


RESULTS
Compared with the EP group, CSF glucose and pyruvate levels were higher (all P<0.01), and lactate levels were lower in the Sfigroup (P<0.01). Meanwhile, CSF glutamate and glycerol levels in the Sfigroup were lower in comparison to the EP group (all P<0.05). In addition, Sfidecreased NDS at 24 h after ROSC (P<0.01), and alleviated the histopathological damage of the brain.


CONCLUSIONS
Sficould alleviate brain injury after CA, which may be associated with improving cerebral metabolism.
Department of Emergency, the First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
Qin
Q
Yin
Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University, Beijing Key Laboratory of Cardiopulmonarycerebral Resuscitation, Beijing, 100020, China.
Cai-Jun
CJ
Wu
Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University, Beijing Key Laboratory of Cardiopulmonarycerebral Resuscitation, Beijing, 100020, China.
Jun
J
Yang
Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University, Beijing Key Laboratory of Cardiopulmonarycerebral Resuscitation, Beijing, 100020, China.
Chen-Chen
CC
Hang
Department of Emergency, Beijing Chaoyang Hospital, Capital Medical University, Beijing Key Laboratory of Cardiopulmonarycerebral Resuscitation, Beijing, 100020, China. lcscyyy@163.com.
Chun-Sheng
CS
Li
D000818:Animals; D001775:Blood Circulation; D001784:Blood Gas Analysis; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D016887:Cardiopulmonary Resuscitation; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use*; D006323:Heart Arrest / Q000134:cerebrospinal fluid / Q000188:drug therapy* / Q000503:physiopathology; D007267:Injections; D007601:Jugular Veins / Q000187:drug effects / Q000378:metabolism; D010477:Perfusion; D034421:Sus scrofa
D004365:Drugs, Chinese Herbal; C492793:Shen-Fu
Shenfu Injection; cardiac arrest; cerebral metabolism