Effects of conventional CPB and mini-CPB on neutrophils CD162, CD166 and CD195 expression.
OBJECTIVE
Cardiac surgery is known to trigger a systemic inflammatory response. While the use of conventional cardiopulmonary bypass (CPB) results in profound inflammation, modified mini-CPB is considered less harmful. We evaluated the impact of cardiac surgery on the expression of CD162, CD166, CD195 molecules and their association with the type of CPB used.


RESULTS
Twenty-four patients were enrolled in our study. Twelve of them were operated using conventional CPB while the other twelve patients underwent surgery with mini-CPB. Blood samples were analysed by flow cytometry. We observed a significant increase in median fluorescence intensity of CD162 and CD195 that peaked instantly after surgery and normalized to the baseline value on the 1stday post surgery, whereas CD166 was initially down-regulated and its median fluorescence intensity (MFI) value increased to the baseline in the next few days.


CONCLUSIONS
We observed immediate changes in the expression of CD162, CD166, and CD195 molecules on the neutrophils after surgery in both study groups of patients. The intensity of the observed changes was significantly greater in the group of patients who underwent conventional CPB compared to patients who underwent mini-CPB cardiac surgery.
1 Department of Clinical Immunology, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Kralove, Czech Republic.
Drahomira
D
Holmannova
1 Department of Clinical Immunology, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Kralove, Czech Republic.
Martina
M
Kolackova
2 Department of Cardiac Surgery, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Kralove, Czech Republic.
Jiri
J
Mandak
2 Department of Cardiac Surgery, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Kralove, Czech Republic.
Pavel
P
Kunes
2 Department of Cardiac Surgery, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Kralove, Czech Republic.
Zdenka
Z
Holubcova
3 Division of Cardiovascular Surgery, University Hospital Zurich, Zurich, Switzerland.
Tomas
T
Holubec
1 Department of Clinical Immunology, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Kralove, Czech Republic.
Jan
J
Krejsek
D000368:Aged; D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology; D002315:Cardiopulmonary Bypass / Q000009:adverse effects* / Q000295:instrumentation / Q000379:methods; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis* / Q000276:immunology; D005260:Female; D005326:Fetal Proteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007249:Inflammation / Q000209:etiology* / Q000276:immunology / Q000517:prevention & control; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000276:immunology; D008875:Middle Aged; D019060:Minimally Invasive Surgical Procedures / Q000009:adverse effects* / Q000295:instrumentation / Q000379:methods; D009504:Neutrophils / Q000276:immunology*; D019713:Receptors, CCR5 / Q000032:analysis* / Q000276:immunology; D020410:Activated-Leukocyte Cell Adhesion Molecule
D015703:Antigens, CD; D015816:Cell Adhesion Molecules, Neuronal; D005326:Fetal Proteins; D008562:Membrane Glycoproteins; D019713:Receptors, CCR5; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule; C584933:CCR5 protein, human; C084882:P-selectin ligand protein
CD162; CD166; CD195; cardiopulmonary bypass; inflammation