Pirkle-type chiral stationary phase on core-shell and fully porous particles: Are superficially porous particles always the better choice toward ultrafast high-performance enantioseparations?
Pirkle-type Whelk-O1 chiral stationary phase (CSP) was prepared on 2.6μm superficially porous particles (SPPs). The chromatographic behavior of columns packed with this new CSP was compared with that of columns packed respectively with 1.8 and 2.5μm Whelk-O1 fully porous particles (FPPs). In the comparison, both thermodynamic and kinetic aspects were considered. Contrary to initial expectations, chiral columns packed with 2.6μm SPPs were quasi-comparable to those packed with 2.5μm FPPs, apparently due to larger contributions to band broadening from both eddy dispersion and, especially for the second eluted enantiomer, adsorption-desorption kinetics. These findings raise the question if SPPs, in spite of the undeniable advantages of their morphology to speed up mass transfer, are always the best choice for high-efficient ultrafast chiral separations. The last part of the work focuses on the use of short columns (10mm long) and very high flow rates to realize the separation of the enantiomers of trans-stilbene oxide (TSO) in normal phase mode in less than 1s.
Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy.
Omar H
OH
Ismail
Dept. of Chemistry and Pharmaceutical Sciences, University of Ferrara, via L. Borsari 46, 44121 Ferrara, Italy.
Luisa
L
Pasti
Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy.
Alessia
A
Ciogli
Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy.
Claudio
C
Villani
Regis Technologies, Inc., 8210 Austin Avenue, Morton Grove, IL 60053, USA.
Jelena
J
Kocergin
Regis Technologies, Inc., 8210 Austin Avenue, Morton Grove, IL 60053, USA.
Scott
S
Anderson
Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy. Electronic address: francesco.gasparrini@uniroma1.it.
Francesco
F
Gasparrini
Dept. of Chemistry and Pharmaceutical Sciences, University of Ferrara, via L. Borsari 46, 44121 Ferrara, Italy. Electronic address: cvz@unife.it.
Alberto
A
Cavazzini
Dept. of Chemistry and Pharmaceutical Sciences, University of Ferrara, via L. Borsari 46, 44121 Ferrara, Italy.
Martina
M
Catani
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000592:standards*; D007700:Kinetics; D008970:Molecular Weight; D016062:Porosity; D013237:Stereoisomerism
Mass transfer kinetics; Normal phase mode; Sub-second separation; Ultrafast enantioseparations; Whelk-O1 superficially porous chiral stationary phase