Preparation and characterization of high purity β-chitin from squid pens (Loligo chenisis).
Squid pens were extracted by a NaOH solution at 80°C for 10h to remove protein and minerals. The as-prepared β-chitin had a high molecular weight (8.5±0.1×103kDa), a low protein content (0.63±0.02wt.%), and a negligible amount of minerals. This method avoids the conventional method for the removal of minerals from shrimp and crab shells by HCl. The purity of resulting products was measured by NMR and FTIR. Moreover, the morphology and crystallinity of β-chitin was characterized by SEM and XRD. The β-chitin with long chains and high purity is suitable for producing high quantity β-chitosan for various potential applications.
Faculty of Biotechnology, Binh Duong University, VietNam.
Hoang Ngoc
HN
Cuong
Institute for Biotechnology and Environment, Nha Trang University, VietNam.
Nguyen Cong
NC
Minh
Faculty of Food Technology, Nha Trang University, VietNam.
Nguyen
N
Van Hoa
Faculty of Food Technology, Nha Trang University, VietNam. Electronic address: trungts@ntu.edu.vn.
Trang Si
TS
Trung
D000818:Animals; D002686:Chitin / Q000737:chemistry* / Q000302:isolation & purification*; D049834:Loligo / Q000737:chemistry*
D002686:Chitin
Loligo chenesis; Squid pens; β-Chitin