Augmenting in vitro osteogenesis of a glycine-arginine-glycine-aspartic-conjugated oxidized alginate-gelatin-biphasic calcium phosphate hydrogel composite and in vivo bone biogenesis through stem cell delivery.
A functionally modified peptide-conjugated hydrogel system was fabricated with oxidized alginate/gelatin loaded with biphasic calcium phosphate to improve its biocompatibility and functionality. Sodium alginate was treated by controlled oxidation to transform the cis-diol group into an aldehyde group in a controlled manner, which was then conjugated to the amine terminus of glycine-arginine-glycine-aspartic. Oxidized alginate glycine-arginine-glycine-aspartic was then combined with gelatin-loaded biphasic calcium phosphate to form a hydrogel of composite oxidized alginate/gelatin/biphasic calcium phosphate that displayed enhanced human adipose stem cell adhesion, spreading and differentiation.1H nuclear magnetic resonance and electron spectroscopy for chemical analysis confirmed that the glycine-arginine-glycine-aspartic was successfully grafted to the oxidized alginate. Co-delivery of glycine-arginine-glycine-aspartic and human adipose stem cell in a hydrogel matrix was studied with the results indicating that hydrogel incorporated modified with glycine-arginine-glycine-aspartic and seeded with human adipose stem cell enhanced osteogenesis in vitro and bone formation in vivo.
Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, Korea Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Cheonan, Korea.
Nguyen Tb
NT
Linh
Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, Korea.
Kallyanashis
K
Paul
Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Cheonan, Korea.
Boram
B
Kim
Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, Korea Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Cheonan, Korea lbt@sch.ac.kr.
Byong-Taek
BT
Lee
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001120:Arginine / Q000737:chemistry; D018786:Bone Substitutes / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005780:Gelatin / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D005998:Glycine / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D008297:Male; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010012:Osteogenesis / Q000502:physiology*; D010084:Oxidation-Reduction; D012887:Skull Fractures / Q000503:physiopathology / Q000628:therapy*; D033581:Stem Cell Transplantation / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds; D016896:Treatment Outcome
D000464:Alginates; D018786:Bone Substitutes; D002130:Calcium Phosphates; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid; D005780:Gelatin; D001120:Arginine; C485829:calcium phosphate, dibasic, anhydrous; D005998:Glycine
Glycine–arginine–glycine–aspartic; biphasic calcium phosphate; bone formation; gelatin; human adipose stem cell; osteogenesis; oxidized alginate