Formation of Neural Networks in 3D Scaffolds Fabricated by Means of Laser Microstereolithography.
We developed and tested new 3D scaffolds for neurotransplantation. Scaffolds of predetermined architectonic were prepared using microstereolithography technique. Scaffolds were highly biocompatible with the nervous tissue cells. In vitro studies showed that the material of fabricated scaffolds is not toxic for dissociated brain cells and promotes the formation of functional neural networks in the matrix. These results demonstrate the possibility of fabrication of tissue-engineering constructs for neurotransplantation based on created scaffolds.
N. I. Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, Russia. mvedunova@yandex.ru.|Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia. mvedunova@yandex.ru.
M V
MV
Vedunova
Institute of Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia.
P S
PS
Timashev
N. I. Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, Russia.|Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia.
T A
TA
Mishchenko
N. I. Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, Russia.|Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia.
E V
EV
Mitroshina
Institute of Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia.
A V
AV
Koroleva
Institute of Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia.
B N
BN
Chichkov
Institute of Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia.
V Ya
VY
Panchenko
Institute of Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia.
V N
VN
Bagratashvili
N. I. Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, Russia.|Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia.
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Mukhina
D001672:Biocompatible Materials / Q000737:chemistry*; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials
3D polymeric scaffolds; calcium imaging; dissociated hippocampal cultures; microstereolithography; two-photon polymerization