Hydrolytic enzyme activities in shiitake mushroom (Lentinula edodes) strains cultivated on coffee pulp.
Hydrolytic enzyme production (cellulases, laminarinases and xylanases) was studied in cultures of Lentinula edodes on sterilized coffee pulp. Samples of substrate colonized by mycelia were taken after 7, 14, 21, 28 and 35 days of incubation at 25°C (W1 to W5) and during the fruiting period at different stages: formation of primordia (PF), first harvest (H) and one week after the first harvest (PH). The enzymatic activity was lower during the early mycelial growth and showed higher levels during the formation and development of fruiting bodies. During the reproductive stage of the fungus, the samples were subjected to a soaking treatment; however, it was not possible to relate this soaking treatment to the increase in enzyme production. The levels of enzymatic activity suggest that secretion of the studied enzymes does not influence the adaptability of the strains to the substrate.
Red Manejo Biotecnológico de Recursos, Instituto de Ecología, Xalapa, Veracruz, Mexico.
Gerardo
G
Mata
Red Manejo Biotecnológico de Recursos, Instituto de Ecología, Xalapa, Veracruz, Mexico. Electronic address: dulce.salmones@inecol.mx.
Dulce
D
Salmones
Red Manejo Biotecnológico de Recursos, Instituto de Ecología, Xalapa, Veracruz, Mexico.
Rosalía
R
Pérez-Merlo
D000222:Adaptation, Physiological; D040503:Coffea; D003470:Culture Media; D005656:Fungal Proteins / Q000378:metabolism*; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D007220:Industrial Waste; D025282:Mycelium / Q000201:enzymology; D012098:Reproduction; D020942:Shiitake Mushrooms / Q000201:enzymology* / Q000254:growth & development; D013045:Species Specificity
D003470:Culture Media; D005656:Fungal Proteins; D007220:Industrial Waste; D006867:Hydrolases
Cellulases; Celulasas; Coffee wastes; Edible mushrooms; Enzyme production; Hongos comestibles; Laminarinasas; Laminarinases; Producción de enzimas; Residuos de café; Xilanasas; Xylanases