Evaluation of a commercial immunochromatographic assay for rapid routine identification of PBP2a-positive Staphylococcus aureus and coagulase-negative staphylococci.
We evaluated the performance of an immunochromatographic assay (PBP2a Culture Colony Test - Alere™), detecting protein-binding penicillin 2a on staphylococci primary isolates in only 6minutes. The assay is highly sensitive for the direct detection of MRSA on various culture media whereas it requires cefoxitin induction for methicillin-resistant coagulase-negative staphylococci.
National Reference Centre for Staphylococci, International Centre of Infectious Research, INSERM U1111, CNRS UMR5308, University of Lyon 1, ENS de Lyon, Lyon, France; Departments of Clinical Microbiology, Hospices Civils de Lyon, Lyon, France. Electronic address: celine.dupieux@chu-lyon.fr.
Céline
C
Dupieux
National Reference Centre for Staphylococci, International Centre of Infectious Research, INSERM U1111, CNRS UMR5308, University of Lyon 1, ENS de Lyon, Lyon, France.
Sophie
S
Trouillet-Assant
National Reference Centre for Staphylococci, International Centre of Infectious Research, INSERM U1111, CNRS UMR5308, University of Lyon 1, ENS de Lyon, Lyon, France.
Jason
J
Tasse
Departments of Clinical Microbiology, Hospices Civils de Lyon, Lyon, France.
Anne-Marie
AM
Freydière
Departments of Clinical Microbiology, Hospices Civils de Lyon, Lyon, France.
Olivia
O
Raulin
Departments of Clinical Microbiology, Hospices Civils de Lyon, Lyon, France.
Chantal
C
Roure-Sobas
Departments of Clinical Microbiology, Hospices Civils de Lyon, Lyon, France.
Hélène
H
Salord
Departments of Clinical Microbiology, Hospices Civils de Lyon, Lyon, France.
Sylvestre
S
Tigaud
National Reference Centre for Staphylococci, International Centre of Infectious Research, INSERM U1111, CNRS UMR5308, University of Lyon 1, ENS de Lyon, Lyon, France; Departments of Clinical Microbiology, Hospices Civils de Lyon, Lyon, France.
Frédéric
F
Laurent
D000900:Anti-Bacterial Agents / Q000378:metabolism; D002440:Cefoxitin / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods*; D016106:Methicillin Resistance; D046915:Penicillin-Binding Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013210:Staphylococcus / Q000737:chemistry* / Q000187:drug effects; D013997:Time Factors; D015533:Transcriptional Activation
D000900:Anti-Bacterial Agents; D046915:Penicillin-Binding Proteins; D002440:Cefoxitin
Methicillin resistance; PBP2a detection; Rapid test; Staphylococci