The Methoxyflavonoid Isosakuranetin Suppresses UV-B-Induced Matrix Metalloproteinase-1 Expression and Collagen Degradation Relevant for Skin Photoaging.
Solar ultraviolet (UV) radiation is a main extrinsic factor for skin aging. Chronic exposure of the skin to UV radiation causes the induction of matrix metalloproteinases (MMPs), such as MMP-1, and consequently results in alterations of the extracellular matrix (ECM) and skin photoaging. Flavonoids are considered as potent anti-photoaging agents due to their UV-absorbing and antioxidant properties and inhibitory activity against UV-mediated MMP induction. To identify anti-photoaging agents, in the present study we examined the preventative effect of methoxyflavonoids, such as sakuranetin, isosakuranetin, homoeriodictyol, genkwanin, chrysoeriol and syringetin, on UV-B-induced skin photo-damage. Of the examined methoxyflavonoids, pretreatment with isosakuranetin strongly suppressed the UV-B-mediated induction of MMP-1 in human keratinocytes in a concentration-dependent manner. Isosakuranetin inhibited UV-B-induced phosphorylation of mitogen-activated protein kinase (MAPK) signaling components, ERK1/2, JNK1/2 and p38 proteins. This result suggests that the ERK1/2 kinase pathways likely contribute to the inhibitory effects of isosakuranetin on UV-induced MMP-1 production in human keratinocytes. Isosakuranetin also prevented UV-B-induced degradation of type-1 collagen in human dermal fibroblast cells. Taken together, our findings suggest that isosakuranetin has the potential for development as a protective agent for skin photoaging through the inhibition of UV-induced MMP-1 production and collagen degradation.
Graduate School of East-West Medical Sciences, Kyung Hee University, Yongin 17104, Korea. sbsbc@khu.ac.kr.
Hana
H
Jung
Graduate School of East-West Medical Sciences, Kyung Hee University, Yongin 17104, Korea. ehwang@khu.ac.kr.
Eunjoo H
EH
Lee
Graduate School of East-West Medical Sciences, Kyung Hee University, Yongin 17104, Korea. thlee@khu.ac.kr.
Tae Hoon
TH
Lee
Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea. manhocho@khu.ac.kr.
Man-Ho
MH
Cho
D002460:Cell Line; D003094:Collagen / Q000378:metabolism*; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D059748:Proteolysis; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects; D062327:Skin Lightening Preparations / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D005419:Flavonoids; D062327:Skin Lightening Preparations; D003094:Collagen; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; C538973:isosakuranetin
MMP-1; UV-B-radiation; anti-photoaging; isosakuranetin; methoxyflavonoid