Compartmentalization of the endoplasmic reticulum in the early C. elegans embryos.
The one-cell Caenorhabditis elegans embryo is polarized to partition fate determinants between the cell lineages generated during its first division. Using fluorescence loss in photobleaching, we find that the endoplasmic reticulum (ER) of the C. elegans embryo is physically continuous throughout the cell, but its membrane is compartmentalized shortly before nuclear envelope breakdown into an anterior and a posterior domain, indicating that a diffusion barrier forms in the ER membrane between these two domains. Using mutants with disorganized ER, we show that ER compartmentalization is independent of the morphological transition that the ER undergoes in mitosis. In contrast, compartmentalization takes place at the position of the future cleavage plane in a par-3-dependent manner. Together, our data indicate that the ER membrane is compartmentalized in cells as diverse as budding yeast, mouse neural stem cells, and the early C. elegans embryo.
Department of Biology, Institute of Biochemistry, Swiss Federal Institute of Technology Zürich, CH-8093 Zürich, Switzerland.
Zuo Yen
ZY
Lee
Department of Cell Physiology and Metabolism, University of Geneva Medical School, CH-1211 Geneva, Switzerland.
Manoël
M
Prouteau
Department of Cell Physiology and Metabolism, University of Geneva Medical School, CH-1211 Geneva, Switzerland.
Monica
http://orcid.org/0000-0002-4336-7860
M
Gotta
Department of Biology, Institute of Biochemistry, Swiss Federal Institute of Technology Zürich, CH-8093 Zürich, Switzerland yves.barral@bc.biol.ethz.ch.
Yves
http://orcid.org/0000-0002-0989-3373
Y
Barral
D000818:Animals; D016880:Anisotropy; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D016764:Cell Polarity; D004058:Diffusion; D004625:Embryo, Nonmammalian / Q000502:physiology; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000502:physiology*; D036681:Fluorescence Recovery After Photobleaching; D005838:Genotype; D008856:Microscopy, Fluorescence; D008938:Mitosis; D009154:Mutation; D009685:Nuclear Envelope / Q000502:physiology; D038081:Organogenesis; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D029742:Caenorhabditis elegans Proteins; D011993:Recombinant Fusion Proteins; C097238:PAR-3 protein, C elegans; D017346:Protein Serine-Threonine Kinases