Influence of autologous dendritic cells on cytokine-induced killer cell proliferation, cell phenotype and antitumor activityin vitro.
Dendritic cell (DCs) are essential antigen processing and presentation cells that play a key role in the immune response. In this study, DCs were co-cultured with cytokine-induced killer cells (DC-CIKs)in vitroto detect changes in cell proliferation, cell phenotype and cell cytotoxicity. The results revealed that the DCs were suitable for co-culture with CIKs at day 7, and that cell quantity of DC-CIKs was lower than that of CIKs until day 11, but it was significantly improved to 1.17-fold that of CIKs at day 13. Flow cytometry was used to detect the cell phenotype of CIKs and DC-CIKs. Compared with CIKs at day 13, the percentage of CD3+, CD3+CD4+, CD3+CD8+and CD3+CD56+T cells in DC-CIKs was significantly improved 1.02, 1.79, 1.26 and 2.44-fold, respectively. In addition, trypan blue staining analysis demonstrated that the cell viability of CIKs and DC-CIKs was 96% and 98%, respectively. Furthermore, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis verified that CIK and DC-CIK cytotoxicity in Hela cells was 58% and 80%, respectively, with a significant difference. Taken together, our results indicate that the cell proliferation, cell phenotype and antitumor activity of CIKs were all enhanced following co-culture with DCsin vitro. These results are likely to be useful for DC-CIK application in antitumor therapies.
Shenzhen Hornetcorn Biotechnology Co. Ltd, Shenzhen, Guangdong 518045, P.R. China.
Jingsong
J
Cao
Laboratory Department, The Second Affliated Hospital of Nanhua University, Hengyang, Hunan 421001, P.R. China.
Cong
C
Chen
Shenzhen Hornetcorn Biotechnology Co. Ltd, Shenzhen, Guangdong 518045, P.R. China.
Yuhuan
Y
Wang
MCH Hospital of Zhuhui, Hengyang, Hunan 421001, P.R. China.
Xuecheng
X
Chen
Changsha Medical University, Changsha, Hunan 410219, P.R. China.
Zeying
Z
Chen
Shenzhen Hornetcorn Biotechnology Co. Ltd, Shenzhen, Guangdong 518045, P.R. China.
Xiaoling
X
Luo
MTT; cytokine-induced killer cells; dendritic cells; flow cytometry