[The effect of shifting of estrogen level on the expression of estrogen receptor and morphology of condyle in rat].
OBJECTIVE
To explore the effect of cinnamaldehyde(CA) on osteogenic function and BMP-2 expression of mice osteoblast.


METHODS
MC3T3-E1 sub-clone 14 cells were cultured in the presence of CA at 0(control group), 10, 20, 40, 80, 160， 320 μg/mL. The cell proliferation rate was measured by MTT assay 1, 3, 5 d after exposure. Activity of alkaline phosphatase (ALP) was detected at 3, 5, 7 d to observe the effect of 6 concentrations of CA on osteoblast function. The mRNA expression of BMP-2 was quantified by real-time PCR(RT-qPCR) 1, 3, 5 d after exposure to (10,20 μg/mL) CA. The data were analyzed by SPSS 17 software package.


RESULTS
MTT assay showed that compared with the control group, when 10, 20 μg/mL of CA acted on MC3T3, the absorbance value decreased slightly but the difference was not statistically significant (P>0.05), while ALP activity increased significantly. CA increased the mRNA expression of BMP-2 at the concentration of 10, 20 μg/mL (P<0.05),decreased the osteoblast proliferation, ALP activity and the mRNA expression of BMP-2 at the concentration of 40, 80, 160， 320 μg/mL (P<0.05); as the concentration of CA increased, the effects became more significant.


CONCLUSIONS
CA(10, 20 μg/mL) exerted positive effects on osteogenic function, increased the ALP activity and the mRNA expression of BMP-2. It is suggested that the activity of osteoblast differentiation and osteogenic function may be related to the expression of BMP-2.
Department of Prosthodontics, Affiliated Hospital to Binzhou Medical University. Binzhou 256603, Shandong Province, China. E-mail:haowenxiu89@163.com.
Wen-Xiu
WX
Hao
Yun-Tao
YT
Zhang
Ya-Jie
YJ
Zhang
D000469:Alkaline Phosphatase; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D004967:Estrogens / Q000378:metabolism*; D008335:Mandibular Condyle / Q000033:anatomy & histology* / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts; D010012:Osteogenesis; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D011960:Receptors, Estrogen / Q000378:metabolism*
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D004967:Estrogens; D011960:Receptors, Estrogen; D000469:Alkaline Phosphatase