Communication between nitric oxide synthase and positively-charged surface and bone formation promotion.
Despite the effects on physiology of bone marrow mesenchymal stem cells (BMSCs) and bone tissue, biological signal communication between bone implants and them is seldom employed as a guidance to create an osteo-inductive interface. Herein, the positively-charged surface is constructed on bone implant from the perspective of mediation of nitric oxide synthase (NOS) expression to signal BMSCs osteo-differentiation. In vitro and in vivo results indicate that the proper surface potential on the positively-charged surface affects NOS to express a high level of inducible nitric oxide synthase (iNOS) in three NOS isoforms of the contacted BMSCs, upregulates their osteogenetic expression, and ultimately foster new bone growth. However, an excessively high surface potential produces substantial immunomodulatory effects thereby offsetting the aforementioned advantages. This study demonstrates that fine-tuning of the positively-charged surface and proper utilization of the communication between NOS and bone implants promote bone formation.
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: weizhang@mail.ipc.ac.cn.
Wei
W
Zhang
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Jun
J
Liu
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Haigang
H
Shi
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Kun
K
Yang
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Pingli
P
Wang
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Gexia
G
Wang
Stomatology Department of the General Hospital of Chinese PLA, 28 FuXing Road, Beijing 100853, China.
Na
N
Liu
Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, Guangdong, China.
Huaiyu
H
Wang
Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: jhji@mail.ipc.ac.cn.
Junhui
J
Ji
Department of Physics & Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
Paul K
PK
Chu
D000818:Animals; D001846:Bone Development; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties
D019001:Nitric Oxide Synthase
NOS; Orthopedic implant; Osteogenesis; Positively-charged surface; Surface potential