Regulation of starvation-induced hyperactivity by insulin and glucagon signaling in adultDrosophila.
Starvation induces sustained increase in locomotion, which facilitates food localization and acquisition and hence composes an important aspect of food-seeking behavior. We investigated how nutritional states modulated starvation-induced hyperactivity in adultDrosophila. The receptor of the adipokinetic hormone (AKHR), the insect analog of glucagon, was required for starvation-induced hyperactivity. AKHR was expressed in a small group of octopaminergic neurons in the brain. Silencing AKHR+neurons and blocking octopamine signaling in these neurons eliminated starvation-induced hyperactivity, whereas activation of these neurons accelerated the onset of hyperactivity upon starvation. Neither AKHR nor AKHR+neurons were involved in increased food consumption upon starvation, suggesting that starvation-induced hyperactivity and food consumption are independently regulated. Single cell analysis of AKHR+neurons identified the co-expression ofDrosophilainsulin-like receptor (dInR), which imposed suppressive effect on starvation-induced hyperactivity. Therefore, insulin and glucagon signaling exert opposite effects on starvation-induced hyperactivity via a common neural target inDrosophila.
Life Sciences Institute, Zhejiang University, Hangzhou, China.|Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
Yue
Y
Yu
Life Sciences Institute, Zhejiang University, Hangzhou, China.|Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
Rui
R
Huang
Life Sciences Institute, Zhejiang University, Hangzhou, China.|Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
Jie
J
Ye
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.
Vivian
V
Zhang
Life Sciences Institute, Zhejiang University, Hangzhou, China.|Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
Chao
C
Wu
Life Sciences Institute, Zhejiang University, Hangzhou, China.|Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
Guo
G
Cheng
Life Sciences Institute, Zhejiang University, Hangzhou, China.|Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
Junling
J
Jia
Life Sciences Institute, Zhejiang University, Hangzhou, China.|Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, China.
Liming
http://orcid.org/0000-0001-7758-1120
L
Wang
AKHR; D. melanogaster; Drosophila; dInR; food seeking; neuroscience; octopamine