Proteomic profile of mouse fibroblasts exposed to pure magnesium extract.
Magnesium and its alloys gain wide attention as degradable biomaterials. In order to reveal the molecular mechanism of the influence of biodegradable magnesium on cells, proteomics analysis was performed in this work. After mouse fibroblasts (L929) were cultured with or without Mg degradation products (Mg-extract) for 8, 24, and 48h, changes in protein expression profiles were obtained using isobaric tags for relative and absolute quantitation (iTRAQ) coupled two dimensional liquid chromatography-tandem mass spectrometry (2D LC MS/MS). A total of 867 proteins were identified (relying on at least two peptides). Compared to the control group, 205, 282, and 217 regulated proteins were identified at 8, 24, and 48h, respectively. 65 common proteins were up or down- regulated within all the three time points, which were involved in various physiological and metabolic activities. Consistent with viability, proliferation, and cell cycle analysis, stimulated energy metabolism as well as protein synthesis pathways were discussed, indicating a possible effect of Mg-extract on L929 proliferation. Furthermore, endocytosis and focal adhesion processes were also discussed. This proteomics study uncovers early cellular mechanisms triggered by Mg degradation products and highlights the cytocompatibility of biodegradable metallic materials for biomedical applications such as stents or orthopaedic implants.
Shenzhen Institute, Peking University, Shenzhen 518057, China; College of Engineering, Peking University, Beijing 100871, China.
Zhen
Z
Zhen
Institute of Material Research, Helmholtz-Zentrum Geesthacht, Hamburg 21502, Germany. Electronic address: berengere.luthringer@hzg.de.
Bérengère
B
Luthringer
College of Engineering, Peking University, Beijing 100871, China.
Li
L
Yang
Shenzhen Institute, Peking University, Shenzhen 518057, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: xitingfei@pku.edu.cn.
Tingfei
T
Xi
Shenzhen Institute, Peking University, Shenzhen 518057, China; College of Engineering, Peking University, Beijing 100871, China.
Yufeng
Y
Zheng
Institute of Material Research, Helmholtz-Zentrum Geesthacht, Hamburg 21502, Germany.
Frank
F
Feyerabend
Institute of Material Research, Helmholtz-Zentrum Geesthacht, Hamburg 21502, Germany.
Regine
R
Willumeit
Shenzhen Institute, Peking University, Shenzhen 518057, China.
Chen
C
Lai
College of Engineering, Peking University, Beijing 100871, China.
Zigang
Z
Ge
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology*; D051379:Mice; D010085:Oxidative Phosphorylation / Q000187:drug effects; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000187:drug effects
D003470:Culture Media; D008274:Magnesium
Biodegradable material; Magnesium; Mouse fibroblasts; Proteomics