Lipid Head Group Charge and Fatty Acid Configuration Dictate Liposome Mobility in Neurofilament Networks.
Intermediate filaments constitute a class of biopolymers whose function is still poorly understood. One example for such intermediate filaments is given by neurofilaments, large macromolecules that fill the axon of neurons. Here, reconstituted networks of purified porcine neurofilaments are studied and the diffusion behavior of different nanoparticles in the biopolymer network is evaluated. A strong dependence of particle diffusion on the charge state of the particles, and - for liposomes - also on the fatty acid configuration of lipids is observed. The results suggest that both electrostatic and hydrophobic interactions contribute to nanoparticle trapping in neurofilament networks, and that the latter is enabled by lipids with an inverted cone geometry which grant access to the hydrophobic core of the liposome shell.
Department of Mechanical Engineering and Institute of Medical Engineering (IMETUM), Technical University of Munich, 85748, Garching, Germany.
Fabienna
F
Arends
Nanobiophysics group, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, 7500, AE, Enschede, The Netherlands.
Himanshu
H
Chaudhary
Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, 19104, USA.
Paul
P
Janmey
Nanobiophysics group, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, 7500, AE, Enschede, The Netherlands.
Mireille M A E
MM
Claessens
Department of Mechanical Engineering and Institute of Medical Engineering (IMETUM), Technical University of Munich, 85748, Garching, Germany.
Oliver
O
Lieleg
D000818:Animals; D004058:Diffusion; D005227:Fatty Acids / Q000737:chemistry*; D007382:Intermediate Filaments / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D010316:Particle Size; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties; D034421:Sus scrofa
D005227:Fatty Acids; D008081:Liposomes; D010714:Phosphatidylethanolamines; D011137:Polystyrenes; C094877:dioleoyl phosphatidylethanolamine
axon; diffusion; intermediate filaments; vesicles