Efficient porcine reproductive and respiratory syndrome virus entry in MARC-145 cells requires EGFR-PI3K-AKT-LIMK1-COFILIN signaling pathway.
Viruses have evolved diverse strategies to take over cellular machinery to facilitate their infection. In our studies presented here, we first demonstrated that Src kinase was involved in PRRSV entry in MARC-145 cells. Further studies demonstrated epidermal growth factor receptor (EGFR) was activated by the currently unknown mechanism(s) during PRRSV entry, which subsequently initiated EGFR downstream signal pathways, such as PI3K/AKT/LIMK1. Through these pathways, the virus entry signal was ultimately transferred to cofilin, which might regulate the actin fragmentation and reorganization to facilitate the virus penetration and cytoplasmic trafficking.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
Rui
R
Wang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
Xin
X
Wang
Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China.
Jia-Qiang
JQ
Wu
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
Bo
B
Ni
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Science, Nanjing, China.
Li-Bing
LB
Wen
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
Li
L
Huang
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Ying
Y
Liao
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Guang-Zhi
GZ
Tong
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Chan
C
Ding
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China. Electronic address: xmao@njau.edu.cn.
Xiang
X
Mao
D051339:Actin Depolymerizing Factors / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D066246:ErbB Receptors / Q000378:metabolism*; D054461:Lim Kinases / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000378:metabolism / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction; D013552:Swine; D053586:Virus Internalization; D019061:src-Family Kinases / Q000378:metabolism
D051339:Actin Depolymerizing Factors; D019869:Phosphatidylinositol 3-Kinases; D066246:ErbB Receptors; D019061:src-Family Kinases; D054461:Lim Kinases; D051057:Proto-Oncogene Proteins c-akt
Cofilin; Entry; Epidermal growth factor receptor; Porcine reproductive and respiratory syndrome virus