Phage display-derived oligopeptide-functionalized probes for in vivo specific photoacoustic imaging of osteosarcoma.
Specific detection of various tumor types remains crucial for designing effective treatment strategies. We demonstrate photoacoustic imaging (PAI) using high-affinity and high-specificity peptide-based probes for accurate and specific diagnosis of osteosarcoma. Herein, two new tumor-specific oligopeptides, termed PT6 and PT7, were identified using phage display-based screening on an osteosarcoma cell line (UMR-106). The identified oligopeptides were able to detect clinical osteosarcoma samples on tissue microarrays. Oligopeptide-conjugated PEGylated gold nanorods (PGNR) were designed to specifically target UMR-106 cells. More importantly, PAI revealed that both PGNR-PT6 and PGNR-PT7 could bind selectively to subcutaneous UMR-106 xenografts after systemic administration and enhance the contrast of osteosarcoma images by 170% and 230%, respectively, compared to tumor-bearing mice injected with PGNRs conjugated to scrambled oligopeptides. PAI employing PGNRs conjugated to specifically designed nanoprobes may provide a new method for tumor type-specific diagnosis of osteosarcoma.
Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
Zebin
Z
Ma
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
Huan
H
Qin
Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
Hongjiang
H
Chen
Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
Hailong
H
Yang
Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
Jiankun
J
Xu
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
Sihua
S
Yang
Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China. Electronic address: hjzkm@vip.163.com.
Jun
J
Hu
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China. Electronic address: xingda@scnu.edu.cn.
Da
D
Xing
D000818:Animals; D001859:Bone Neoplasms / Q000000981:diagnostic imaging*; D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D009368:Neoplasm Transplantation; D009842:Oligopeptides; D012516:Osteosarcoma / Q000000981:diagnostic imaging*; D019151:Peptide Library; D061088:Photoacoustic Techniques
D009842:Oligopeptides; D019151:Peptide Library; D006046:Gold
Oligopeptides; Osteosarcoma; Phage display; Photoacoustic imaging