In vitro culture and phenotypic and molecular characterization of gastric stem cells from human stomach.
BACKGROUND
Human gastric mucosa shows continuous self-renewal via differentiation from stem cells that remain poorly characterized.


METHODS
We describe an original protocol for culture of gastric stem/progenitor cells from adult human stomach. The molecular characteristics of cells were studied using TaqMan low-density array and qRT-PCR analyses using the well-characterized H1 and H9 embryonic stem cells as reference. Epithelial progenitor cells were challenged with H. pylori to characterize their inflammatory response.


RESULTS
Resident gastric stem cells expressed specific molecular markers of embryonic stem cells (SOX2, NANOG, and OCT4), as well as others specific to adult stem cells, particularly LGR5 and CD44. We show that gastric stem cells spontaneously differentiate into epithelial progenitor cells that can be challenged with H. pylori. The epithelial progenitor response to H. pylori showed a cag pathogenicity island-dependent induction of matrix metalloproteinases 1 and 3, chemokine (CXCL1, CXCL5, CXCL8, CCL20) and interleukine 33 expression.


CONCLUSIONS
This study opens new outlooks for investigation of gastric stem cell biology and pathobiology as well as host-H. pylori interactions.
Laboratoire Inflammation, Tissus Epithéliaux et Cytokines, Université de Poitiers, Poitiers, France.|Centre Hospitalier Universitaire de Poitiers, Poitiers, France.
Magali
M
Garcia
Centre Hospitalier Universitaire de Poitiers, Poitiers, France.|Inserm U935, Université de Poitiers, Poitiers, France.
Jean-Claude
JC
Chomel
Laboratoire Inflammation, Tissus Epithéliaux et Cytokines, Université de Poitiers, Poitiers, France.
Pascale
P
Mustapha
Laboratoire Inflammation, Tissus Epithéliaux et Cytokines, Université de Poitiers, Poitiers, France.|Centre Hospitalier Universitaire de Poitiers, Poitiers, France.
Cong Tri
CT
Tran
Laboratoire Inflammation, Tissus Epithéliaux et Cytokines, Université de Poitiers, Poitiers, France.|Centre Hospitalier Universitaire de Poitiers, Poitiers, France.
Martine
M
Garnier
Laboratoire Inflammation, Tissus Epithéliaux et Cytokines, Université de Poitiers, Poitiers, France.|Centre Hospitalier Universitaire de Poitiers, Poitiers, France.
Isabelle
I
Paris
Centre Hospitalier Universitaire de Poitiers, Poitiers, France.
Nathalie
N
Quellard
Centre Hospitalier Universitaire de Poitiers, Poitiers, France.
Julie
J
Godet
Laboratoire Inflammation, Tissus Epithéliaux et Cytokines, Université de Poitiers, Poitiers, France.|Centre Hospitalier Universitaire de Poitiers, Poitiers, France.
Julie
J
Cremniter
Inserm U935, Université de Poitiers, Poitiers, France.|Inserm U935, Hôpitaux Universitaires Paris Sud, Hôpital Bicêtre & Paul Brousse, Université Paris-Sud, Villejuif, France.
Annelise
A
Bennaceur-Griscelli
Laboratoire Inflammation, Tissus Epithéliaux et Cytokines, Université de Poitiers, Poitiers, France.|Centre Hospitalier Universitaire de Poitiers, Poitiers, France.
Jean-Claude
JC
Lecron
Inserm U935, Université de Poitiers, Poitiers, France.|Inserm U935, Hôpitaux Universitaires Paris Sud, Hôpital Bicêtre & Paul Brousse, Université Paris-Sud, Villejuif, France.
Ali G
AG
Turhan
Laboratoire Inflammation, Tissus Epithéliaux et Cytokines, Université de Poitiers, Poitiers, France.|Centre Hospitalier Universitaire de Poitiers, Poitiers, France.
Christophe
C
Burucoa
Laboratoire Inflammation, Tissus Epithéliaux et Cytokines, Université de Poitiers, Poitiers, France.
Charles
http://orcid.org/0000-0002-8016-0324
C
Bodet
D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D004847:Epithelial Cells / Q000382:microbiology / Q000502:physiology; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology*; D020869:Gene Expression Profiling; D005819:Genetic Markers; D016480:Helicobacter pylori / Q000472:pathogenicity; D006801:Humans; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000502:physiology*
D005819:Genetic Markers

Helicobacter pylori
; cag pathogenicity island; epithelial progenitor; gastric stem cells; human primary cells; inflammation