Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs.
Mesenchymal stem cells (MSCs) have been accepted as a promising cell source in tissue repair and regeneration. However, the inability to enrich MSCs in target areas limits their wide application. As a result, it has been a major goal to induce MSCs to be abundantly and specifically recruited to the injury site. In this study, a peptide with a specific affinity for MSCs (E7 peptide) was immobilized to a collagen scaffold via a collagen-binding domain (CBD) to construct a functional collagen scaffold. In addition, the hypothesis that this method could recruit MSCs specifically was evaluated in a porcine model. In vivo investigations indicated that due to the immunore-action, the CBD-MSC-peptide collagen scaffold enhanced MSC adhesion and infiltration and promoted wound healing. At day 7 after surgery, we found more infiltrating cells and capillaries in the Collagen/CBD-E7 peptide group compared to the Scaffold group. At day 14, 21 and 28, a faster healing process was observed in the Collagen/CBD-E7 peptide group, with significant differences compared with the other groups (P < 0.05, P < 0.01). The results demonstrate the potential use of targeted therapy to rapidly heal skin wounds.
Department of Burns and Plastic Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008 China.
Huili
H
Wang
Department of Burns and Plastic Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008 China.
Xin
X
Yan
Department of Burns and Plastic Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008 China.
Liangyun
L
Shen
Department of Burns and Plastic Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008 China.
Shiyan
S
Li
Department of Burns and Plastic Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008 China.
Yue
Y
Lin
Department of Burns and Plastic Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008 China.
Shuqin
S
Wang
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Zhongguancun, Beijing, China.
Xiang Lin
XL
Hou
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Zhongguancun, Beijing, China.
Chunying
C
Shi
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Zhongguancun, Beijing, China.
Yun
Y
Yang
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Zhongguancun, Beijing, China.
Jianwu
J
Dai
Department of Burns and Plastic Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210008 China.
Qian
Q
Tan
Collagen-binding domain; Mesenchymal stem cells; mesenchymal stem cells affinity peptide; skin wound healing