Crystal structure of Plasmodium falciparum proplasmepsin IV: the plasticity of proplasmepsins.
Plasmepsin IV from Plasmodium falciparum (PM IV) is a promising target for the development of novel antimalarial drugs. Here, the crystal structure of the truncated zymogen of PM IV (pPM IV), consisting of the mature enzyme plus a prosegment of 47 residues, has been determined at 1.5 Å resolution. pPM IV presents the fold previously described for studied proplasmepsins, displaying closer similarities to proplasmepin IV from P. vivax (pPvPM) than to the other two proplasmepsins from P. falciparum. The study and comparison of the pPM IV structure with the proplasmepsin structures described previously provide information about the similarities and differences in the inactivation-activation mechanisms among the plasmepsin zymogens.
Latvian Institute of Organic Synthesis, Aizkraukles 21, Riga, LV-1006, Latvia.
Rosario
R
Recacha
Latvian Institute of Organic Synthesis, Aizkraukles 21, Riga, LV-1006, Latvia.
Kristaps
K
Jaudzems
Biomedical Research and Study Centre, Ratsupites 1, Riga, LV-1067, Latvia.
Inara
I
Akopjana
Latvian Institute of Organic Synthesis, Aizkraukles 21, Riga, LV-1006, Latvia.
Aigars
A
Jirgensons
Biomedical Research and Study Centre, Ratsupites 1, Riga, LV-1067, Latvia.
Kaspars
K
Tars
D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004792:Enzyme Precursors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D010963:Plasmodium falciparum / Q000737:chemistry* / Q000201:enzymology; D011485:Protein Binding; D000072756:Protein Conformation, alpha-Helical; D000072757:Protein Conformation, beta-Strand; D054730:Protein Interaction Domains and Motifs; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D004792:Enzyme Precursors; D007527:Isoenzymes; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D016282:Aspartic Acid Endopeptidases; C089643:plasmepsin
Plasmodium falciparum; aspartic protease zymogen; malaria; proplasmepsin IV