In Vitro Antimicrobial Bioassays, DPPH Radical Scavenging Activity, and FTIR Spectroscopy Analysis of Heliotropium bacciferum.
The present study deals with the antimicrobial, antioxidant, and functional group analysis of Heliotropium bacciferum extracts. Disc diffusion susceptibility method was followed for antimicrobial assessment. Noteworthy antimicrobial activities were recorded by various plant extracts against antibiotic resistant microorganisms. Plant flower extracts antioxidant activity was investigated against 2, 2-diphenyl-1-picryl hydrazyl radical by ultraviolet spectrophotometer (517 nm). Plant extracts displayed noteworthy radical scavenging activities at all concentrations (25-225 μg/mL). Notable activities were recorded by crude, chloroform and ethyl acetate extracts up to 88.27% at 225 μg/mL concentration. Compounds functional groups were examined by Fourier transform infrared spectroscopic studies. Alkanes, alkenes, alkyl halides, amines, carboxylic acids, amides, esters, alcohols, phenols, nitrocompounds, and aromatic compounds were identified by FTIR analysis. Thin layer chromatography bioautography was carried out for all plant extracts. Different bands were separated by various solvent systems. The results of the current study justify the use of Heliotropium bacciferum in traditional remedial herbal medicines.
Department of Chemistry, Qurtuba University of Science and Information Technology Peshawar, Peshawar 25120, Pakistan.
Sohail
0000-0001-5330-4989
S
Ahmad
Riyadh Community College, King Saud University, Riyadh 11437, Saudi Arabia.
Naser M
0000-0001-9848-4573
NM
AbdEl-Salam
Department of Chemistry, Government College Ara Khel, FR Kohat 26000, Pakistan.
Riaz
0000-0002-2860-467X
R
Ullah
D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D001681:Biological Assay / Q000379:methods; D001713:Biphenyl Compounds / Q000737:chemistry* / Q000302:isolation & purification; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry / Q000302:isolation & purification; D028162:Heliotropium / Q000737:chemistry* / Q000145:classification; D010851:Picrates / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry / Q000145:classification; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
D000900:Anti-Bacterial Agents; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl