Biosynthesis of cell wall mannan in the conidium and the mycelium of Aspergillus fumigatus.
The galactomannan is a major cell wall molecule of Aspergillus fumigatus. This molecule is composed of a linear mannan with a repeating unit composed of four α1,6 and α1,2 linked mannose with side chains of galactofuran. To obtain a better understanding of the mannan biosynthesis in A. fumigatus, it was decided to undertake the successive deletion of the 11 genes which are putative orthologs of the mannosyltransferases responsible for establishing α1,6 and α1,2 mannose linkages in yeast. These deletions did not lead to a reduction of the mannan content of the cell wall of the mycelium of A. fumigatus. In contrast, the mannan content of the conidial cell wall was reduced and this reduction was associated with a partial disorganization of the cell wall leading to defects in conidial survival both in vitro and in vivo.
Département de Mycologie, Unité des Aspergillus, Paris Cedex, France.
Christine
C
Henry
Département de Mycologie, Unité des Aspergillus, Paris Cedex, France.
Thierry
T
Fontaine
Département de Mycologie, Unité des Aspergillus, Paris Cedex, France.
Christoph
C
Heddergott
Département de Mycologie, Unité des Aspergillus, Paris Cedex, France.
Pauline
P
Robinet
Département de Mycologie, Unité des Aspergillus, Paris Cedex, France.
Vishukumar
V
Aimanianda
Département de Mycologie, Unité des Aspergillus, Paris Cedex, France.
Remi
R
Beau
Département de Mycologie, Unité des Aspergillus, Paris Cedex, France.
Anne
A
Beauvais
Département de Mycologie, Unité des Aspergillus, Paris Cedex, France.
Isabelle
I
Mouyna
Plateforme UltraPole, Institut Pasteur, Paris Cedex, France.
Marie-Christine
MC
Prevost
Service de Parasitologie Mycologie, Hôpital de La Pitié-Salpêtrière, Paris, France.
Arnaud
A
Fekkar
Public Health Research Institute, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, New Jersey, USA.
Yanan
Y
Zhao
Public Health Research Institute, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, New Jersey, USA.
David
D
Perlin
Département de Mycologie, Unité des Aspergillus, Paris Cedex, France.
Jean-Paul
JP
Latgé
D000818:Animals; D001228:Aspergillosis / Q000382:microbiology / Q000473:pathology; D001232:Aspergillus fumigatus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005690:Galactose / Q000031:analogs & derivatives; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D054884:Host-Pathogen Interactions; D008351:Mannans / Q000737:chemistry / Q000378:metabolism*; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D008364:Mannosyltransferases / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D025282:Mycelium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence
D005656:Fungal Proteins; D008351:Mannans; C012990:galactomannan; D008364:Mannosyltransferases; D008358:Mannose; D005690:Galactose