Doping control analysis of anabolic steroids in equine urine by gas chromatography-tandem mass spectrometry.
Anabolic steroids are banned substances in equine sports. Gas chromatography-mass spectrometry (GC-MS) has been the traditional technique for doping control analysis of anabolic steroids in biological samples. Although liquid chromatography-mass spectrometry (LC/MS) has become an important technique in doping control, the detection of saturated hydroxysteroids by LC-MS remains a problem due to their low ionization efficiency under electrospray. The recent development in fast-scanning gas-chromatography-triple-quadrupole mass spectrometry (GC-MS/MS) has provided a better alternative with a significant reduction in chemical noise by means of selective reaction monitoring. Herein, we present a sensitive and selective method for the screening of over 50 anabolic steroids in equine urine using gas chromatography-tandem mass spectrometry (GC-MS/MS). Copyright © 2016 John Wiley & Sons, Ltd.
Racing Laboratory, The Hong Kong Jockey Club, Sha Tin Racecourse, Hong Kong, China.
April S Y
ASY
Wong
Racing Laboratory, The Hong Kong Jockey Club, Sha Tin Racecourse, Hong Kong, China.
Gary N W
GNW
Leung
Racing Laboratory, The Hong Kong Jockey Club, Sha Tin Racecourse, Hong Kong, China.
David K K
DKK
Leung
Racing Laboratory, The Hong Kong Jockey Club, Sha Tin Racecourse, Hong Kong, China.
Terence S M
TSM
Wan
D045930:Anabolic Agents / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D001826:Body Fluids / Q000737:chemistry* / Q000378:metabolism; D002853:Chromatography, Liquid; D004300:Doping in Sports / Q000517:prevention & control* / Q000706:statistics & numerical data; D006736:Horses; D006801:Humans; D015813:Substance Abuse Detection; D053719:Tandem Mass Spectrometry; D045165:Testosterone Congeners / Q000032:analysis* / Q000737:chemistry
D045930:Anabolic Agents; D045165:Testosterone Congeners
GC-MS/MS analysis; anabolic steroids; doping control; equine urine