Effect of fibronectin adsorption on osteoblastic cellular responses to hydroxyapatite and alumina.
Initial cellular responses following implantation are important for inducing osteoconduction. We investigated cell adhesion, spreading, proliferation and differentiation of mouse MC3T3-E1 osteoblastic cells on untreated or fibronectin (Fn)-coated discs of hydroxyapatite (HAp) or alpha-type alumina (α-Al2O3). Fn coating significantly enhanced adhesion and spreading of MC3T3-E1 cells on HAp, but did not affect MC3T3-E1 cell proliferation and differentiation on HAp or α-Al2O3. Fn-coated HAp likely does not stimulate pre-osteoblast cells to initiate the process of osteoconduction; however, Fn adsorption might affect the response of inflammatory cells to the implanted material or, in conjunction with other serum proteins, stimulate pre-osteoblast cell proliferation and differentiation. Further studies on the effect of serum proteins in cell culture and the efficacy of Fn-coated HAp and α-Al2O3in vivo are warranted.
Graduate School of Biomedical Engineering, Tohoku University, Sendai 980-8579, Japan. Electronic address: m-kawa@ecei.tohoku.ac.jp.
Masakazu
M
Kawashita
Graduate School of Biomedical Engineering, Tohoku University, Sendai 980-8579, Japan.
Maki
M
Hasegawa
Graduate School of Dentistry, Tohoku University, Sendai 980-8575, Japan.
Tada-Aki
TA
Kudo
Graduate School of Dentistry, Tohoku University, Sendai 980-8575, Japan.
Hiroyasu
H
Kanetaka
Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu 808-0196, Japan.
Toshiki
T
Miyazaki
Japan Fine Ceramics Center, Nagoya 456-8587, Japan.
Masami
M
Hashimoto
D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D000537:Aluminum Oxide / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology*; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects
D005353:Fibronectins; D017886:Durapatite; D000469:Alkaline Phosphatase; D000537:Aluminum Oxide
Alumina; Fibronectin; Hydroxyapatite; MC3T3-E1