Protein encapsulation and release from PEO-b-polyphosphoester templated calcium carbonate particles.
Calcium carbonate particles are promising candidates as proteins carriers for their controlled delivery in the body. The present paper aims at investigating the protein encapsulation by in situ precipitation of calcium carbonate particles prepared by a process based on supercritical CO2and using a new type of degradable well-defined double hydrophilic block copolymers composed of poly(ethylene oxide) and polyphosphoester blocks acting as templating agent for the calcium carbonate. For this study, lysozyme was chosen as a model for therapeutic protein for its availability and ease of detection. It was found that by this green process, loading into the CaCO3microparticles with a diameter about 2μm can be obtained as determined by scanning electron microscopy. A protein loading up to 6.5% active lysozyme was measured by a specific bioassay (Micrococcus lysodeikticus). By encapsulating fluorescent-labelled lysozyme (lysozyme-FITC), the confocal microscopy images confirmed its encapsulation and suggested a core-shell distribution of lysozyme into CaCO3, leading to a release profile reaching a steady state at 59% of release after 90min.
Center for Education and Research on Macromolecules (CERM), University of Liège (ULg),Cesam-RU, Allée du 6 AoÛt, 13, Quartier Agora, Sart Tilman, Building B6a-third floor, B-4000 Liège, Belgium; INSERM U1066, Micro et Nanomedécines Biomimétiques, IBS, University of Angers, 4 rue Larrey, Cedex 9, Angers, 49933, France.
Zeynep
Z
Ergul Yilmaz
INSERM U1066, Micro et Nanomedécines Biomimétiques, IBS, University of Angers, 4 rue Larrey, Cedex 9, Angers, 49933, France.
Thomas
T
Cordonnier
Center for Education and Research on Macromolecules (CERM), University of Liège (ULg),Cesam-RU, Allée du 6 AoÛt, 13, Quartier Agora, Sart Tilman, Building B6a-third floor, B-4000 Liège, Belgium.
Antoine
A
Debuigne
INSERM U1066, Micro et Nanomedécines Biomimétiques, IBS, University of Angers, 4 rue Larrey, Cedex 9, Angers, 49933, France.
Brice
B
Calvignac
Center for Education and Research on Macromolecules (CERM), University of Liège (ULg),Cesam-RU, Allée du 6 AoÛt, 13, Quartier Agora, Sart Tilman, Building B6a-third floor, B-4000 Liège, Belgium. Electronic address: c.jerome@ulg.ac.be.
Christine
C
Jerome
INSERM U1066, Micro et Nanomedécines Biomimétiques, IBS, University of Angers, 4 rue Larrey, Cedex 9, Angers, 49933, France. Electronic address: frank.boury@univ-angers.fr.
Frank
F
Boury
D002119:Calcium Carbonate / Q000737:chemistry*; D011232:Chemical Precipitation; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000008:administration & dosage / Q000737:chemistry
D004337:Drug Carriers; D011108:Polymers; D011506:Proteins; D011092:Polyethylene Glycols; D009113:Muramidase; D002119:Calcium Carbonate
CaCO(3) particles; Polyphosphoester; Protein loading; Release test; Supercritical CO(2)