[Expression and clinical significance of PUMA gene in salivary adenoid cystic carcinoma].
OBJECTIVE
To explore the expression of p53 up-regulated modulator of apoptosis (PUMA) gene in salivary adenoid cystic carcinoma (SACC) and its clinical significance.


METHODS
The expressions of PUMA gene and protein were detected in 27 SACC tumor-adjacent tissues (group A) and 27 SACC tumor tissues (group B) with real-time quantitative PCR and Western blot. Statistical analysis was performed using SPSS 19.0 software package.


RESULTS
The expression of PUMA gene in groups A as calibrator was 1，and expression of PUMA gene in group B was 0.57±0.17. The expression of PUMA protein in groups A and B were 0.94±0.12 and 0.36±0.12，respectively. The expressions of PUMA gene and protein were significantly lower in group B than in group A (P<0.05).


CONCLUSIONS
The expression of puma gene in SACC tissues is down-regulated, which is negatively correlated with the development of SACC.
Department of Stomatology, Affiliated Hospital of Taishan Medical University. Tai'an 271000, China. E-mail:rqking315@163.com.
Yin-Tao
YT
Lei
Jun-Fu
JF
Li
Juan
J
Jiang
Xiao-Guang
XG
Li
Dai-Yun
DY
Chen
D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D003528:Carcinoma, Adenoid Cystic / Q000175:diagnosis / Q000378:metabolism*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D006801:Humans; D011506:Proteins; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D015854:Up-Regulation
D051017:Apoptosis Regulatory Proteins; C438378:BBC3 protein, human; D011506:Proteins; D011518:Proto-Oncogene Proteins