Phylogeography of Diptychus maculatus (Cyprinidae) endemic to the northern margin of the QTP and Tien Shan region.
Phylogeography and historical demography of the cyprinid fish Diptychus maculatus (subfamily Schizothoracinae) are evaluated across three river systems in the Northern Qinghai-Tibetan Plateau (QTP) and Tien Shan range: the Indus River, Tarim River and Ili River.



Results from both mtDNA (16S rRNA, Cyt b and D-loop) and nucDNA (RAG-2) resolved four reciprocally monophyletic clades, representing populations from Indus River, South Tarim River, North Tarim River and Ili River, respectively. The divergence times was estimated to be 1.5-2.5 Mya. It is consistent with the hypothesis that the split of four clades is the consequence of vicariance resulting from both the intensive uplift of QTP and Tien Shan as well as the resultant expansion of the Taklimakan Desert. Several lines of evidences indicate dynamic demographic histories for the populations, with late Pleistocene and Holocene population bottlenecks and expansions except the Indus River.



Our results clearly depicted the phylogenetic relationship of D. maculatus from Indus River, Tarim River and Ili River. The analyses implicated the relationship among the distribution of D. maculatus, paleo-drainages and geographic events, and implied the existence of the South Tarim River in history.
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23 Xinning Road, Xining, Qinghai, 810001, China.|Laboratory of Plateau Fish Evolutionary and Functional Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23 Xinning Road, Xining, Qinghai, 810001, China.|University of Chinese Academy of Sciences, Beijing, China.
Guogang
G
Li
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23 Xinning Road, Xining, Qinghai, 810001, China.|Laboratory of Plateau Fish Evolutionary and Functional Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23 Xinning Road, Xining, Qinghai, 810001, China.|University of Chinese Academy of Sciences, Beijing, China.
Yongtao
Y
Tang
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23 Xinning Road, Xining, Qinghai, 810001, China.|Laboratory of Plateau Fish Evolutionary and Functional Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23 Xinning Road, Xining, Qinghai, 810001, China.|University of Chinese Academy of Sciences, Beijing, China.
Renyi
R
Zhang
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23 Xinning Road, Xining, Qinghai, 810001, China. zhaokai@nwipb.cas.cn.|Laboratory of Plateau Fish Evolutionary and Functional Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23 Xinning Road, Xining, Qinghai, 810001, China. zhaokai@nwipb.cas.cn.
Kai
K
Zhao
D063147:Animal Distribution; D000818:Animals; D001499:Bayes Theorem; D003530:Cyprinidae / Q000145:classification / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D003710:Demography; D014644:Genetic Variation; D010802:Phylogeny; D058974:Phylogeography; D012336:RNA, Ribosomal, 16S; D045483:Rivers; D018609:Tibet
D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S
Diptychus maculatus; Mitochondrial and nuclear markers; Phylogeography; Qinghai-Tibetan Plateau; Tienshan Mountains