Long Noncoding RNA ROR Regulates Proliferation, Invasion, and Stemness of Gastric Cancer Stem Cell.
Gastric cancer remains an incurable malignance and the second leading cause of cancer death globally. Recent progress in gastric cancer research has demonstrated the crucial roles of cancer stem cells (CSCs) in the development, metastasis, and drug resistance of this disease. Various studies have highlighted the role of long noncoding RNAs (lncRNAs) in the pathogenesis of gastric cancer. In this study, through fluorescence-activated cell sorting, we isolated gastric CSCs (GCSCs) from MKN-45 cells and demonstrated for the first time that lncRNA ROR was highly expressed in CD133+GCSCs. Overexpression of lncRNA ROR significantly increased, but knockdown of lncRNA ROR inhibited the proliferation and invasion of GCSCs. Most importantly, lncRNA ROR led to upregulation of several key stemness transcriptional factors, such as OCT4, SOX2, and NANOG, as well as CD133 GCSC. Our data demonstrated that lncRNA ROR was associated with core stemness transcriptional factors and the pluripotent state of GCSCs. These results further improved our understanding of the functional cross talking network during development of GCSCs and may provide novel target for the diagnostics and therapeutics of gastric cancer.
1 Department of Clinical Medicine, Shandong University , Jinan, Shandong, China .|2 Department of Oncology, Weifang Traditional Chinese Hospital , Weifang, Shandong, China .
Shuai
S
Wang
3 Department of Medical Imaging, WeiFang Medical University , Weifang, Shandong, China .
Feng
F
Liu
2 Department of Oncology, Weifang Traditional Chinese Hospital , Weifang, Shandong, China .
Junji
J
Deng
2 Department of Oncology, Weifang Traditional Chinese Hospital , Weifang, Shandong, China .
Xinsheng
X
Cai
4 Department of Tumor Research and Therapy Center, Shandong Provincial Hospital Affiliated to Shandong University , Jinan, Shandong, China .
Junqing
J
Han
5 Institute of Oncology, Provincial Hospital Affiliated to Shandong University , Jinan, Shandong, China .
Qi
Q
Liu
D000328:Adult; D000368:Aged; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D062085:RNA, Long Noncoding / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
C000588825:Linc-RNA-RoR, human; D062085:RNA, Long Noncoding