Increased CD69 Expression on Peripheral Eosinophils from Patients with Food Protein-Induced Enterocolitis Syndrome.
BACKGROUND
Food protein-induced enterocolitis syndrome (FPIES) is an uncommon, non-IgE-mediated food allergy. We recently described a significant increase in fecal eosinophil-derived neurotoxin (EDN) after ingestion of the causative food. However, little is known about the activation status of circulating eosinophils in patients with an acute FPIES reaction.


METHODS
Surface CD69 expression was assessed by flow cytometry on peripheral eosinophils from 5 patients with FPIES before and after ingestion of the causative food. Fecal EDN was measured by enzyme-linked immunosorbent assay.


RESULTS
No eosinophil activation was observed before ingestion; however, a significant increase in CD69 expression on eosinophils after an acute FIPES reaction was demonstrated in all of the patients. There was no significant change in absolute eosinophil counts in the peripheral blood. The levels of fecal EDN increased on the day after ingestion of the causative food in all patients.


CONCLUSIONS
These results suggest that circulating eosinophils as well as eosinophils in the intestinal mucosal tissue are activated in acute FPIES reactions and might be associated with systemic immune events in FPIES.
Department of Pediatrics, School of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
Taizo
T
Wada
Yusuke
Y
Matsuda
Tomoko
T
Toma
Eiko
E
Koizumi
Hiroyuki
H
Okamoto
Akihiro
A
Yachie
D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000378:metabolism*; D002675:Child, Preschool; D004044:Dietary Proteins / Q000009:adverse effects*; D004760:Enterocolitis / Q000175:diagnosis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007223:Infant; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D008297:Male; D010641:Phenotype; D013577:Syndrome; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D004044:Dietary Proteins; D007073:Immunoglobulin E; D037181:Lectins, C-Type; D000099192:CD69 Antigens