Enzymatic production of 5'-inosinic acid by AMP deaminase from a newly isolated Aspergillus oryzae.
5'-adenylic acid deaminase (AMP deaminase), an important enzyme for the food industry, can catalyze the irreversible hydrolysis of adenosine monophosphate (AMP) to inosine monophosphate (IMP) and ammonia. In this study, a new strain was screened that efficiently produces 3191.6U/g of AMP deaminase at 32°C. After purification, the optimal temperature and pH of the AMP deaminase were found to be 40°C and 6.0, respectively, but it was partially inhibited by Fe(3+), Cu(2+), Al(3+), and Zn(2+). With amplification of the AMP deaminase production system, 6mL of crude enzyme could produce 2.00mg/g of IMP from 2.04mg/g of dried yeast with an 84.8% molar yield after 40min. These results provide a new insight into AMP deaminase production and offer a potential platform for producing 5'-IMP.
College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China.
Shubo
S
Li
College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Leitao
L
Chen
College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Yangjun
Y
Hu
College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Guohui
G
Fang
College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China.
Mouming
M
Zhao
National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning 530007, China. Electronic address: guoyuan@gxas.cn.
Yuan
Y
Guo
College of Life Science and Technology, Guangxi University, Nanning 530004, China. Electronic address: pangzw@gxu.edu.cn.
Zongwen
Z
Pang
D000659:AMP Deaminase / Q000032:analysis* / Q000096:biosynthesis*; D001236:Aspergillus oryzae / Q000302:isolation & purification*; D004789:Enzyme Activation / Q000502:physiology; D007291:Inosine Monophosphate / Q000032:analysis* / Q000096:biosynthesis*
D007291:Inosine Monophosphate; D000659:AMP Deaminase
AMP deaminase; Adenosine monophosphate; Aspergillus oryzae; Enzymatic production; Inosine monophosphate