A mild, rapid synthesis of freebase [11C]nicotine from [11C]methyl triflate.
A rapid, mild radiosynthesis of freebase [11C]nicotine was developed by the methylation of freebase nornicotine with [11C]methyl triflate in acetone (5min, 45°C). A basic (pH 10.5-11.0) HPLC system reproducibly yielded freebase [11C]nicotine as a well-defined single peak. The freebase [11C]nicotine was concentrated by solid phase extraction and formulated in 50µL ethanol (370MBq/50µL) without evaporative loss suitable for a cigarette spiking study. A radiochemical yield of 60.4±4.7% (n=3), radiochemical purity ≥99.9% and specific activity of 648GBq/µmol at EOB for 5min beams were achieved.
Biology Department, Brookhaven National Laboratory, Upton, 11973, NY, USA.
Youwen
Y
Xu
Biology Department, Brookhaven National Laboratory, Upton, 11973, NY, USA.
Sung Won
SW
Kim
Biology Department, Brookhaven National Laboratory, Upton, 11973, NY, USA.
Dohyun
D
Kim
Biology Department, Brookhaven National Laboratory, Upton, 11973, NY, USA.
David
D
Alexoff
Biology Department, Brookhaven National Laboratory, Upton, 11973, NY, USA.
Michael J
MJ
Schueller
Biology Department, Brookhaven National Laboratory, Upton, 11973, NY, USA. Electronic address: fowler@bnl.gov.
Joanna S
JS
Fowler
Freebase [(11)C]nicotine; [(11)C]methyl triflate