Arsenic mediated modifications in Bacillus aryabhattai and their biotechnological applications for arsenic bioremediation.
The present study reports the arsenic (As) tolerance mechanism of bacteria Bacillus aryabhattai (NBRI014). The data explores the intracellular accumulation and volatilization of As from the culture medium after 48 h of exposure to 25,000 mg l-1arsenate As(V). The study also provides the evidence of presence of ars operon in bacteria, which may have played an important role in reducing As toxicity. Additionally, we found 7 differentially expressed proteins to be up-regulated in bacterial cells upon As exposure which may have role in reducing As toxicity inside bacterial cells. Furthermore, Fourier transform infrared (FTIR) spectroscopic techniques were useful to describe the structural and compositional alterations in bacterial cells after As treatment. It showed the changes in peak positions of the spectrum pattern when NBRI014 was grown in medium containing As, indicating that these functional groups viz. (amino, alkyl halides and hydroxyl) present on bacterial surface, which may be involved in As binding. The above results signify that biotechnological application of the isolate NBRI014 could be helpful in removal of As from polluted sites.
Eco-auditing Group, CSIR-National Botanical Research Institute, Lucknow, India.
Namrata
N
Singh
Plant Ecology and Environmental Science, CSIR-National Botanical Research Institute, Lucknow, India.
Sunil
S
Gupta
Eco-auditing Group, CSIR-National Botanical Research Institute, Lucknow, India.
Naina
N
Marwa
Plant Ecology and Environmental Science, CSIR-National Botanical Research Institute, Lucknow, India.
Vivek
V
Pandey
Plant Molecular Biology & Genetic Engineering Division, National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, UP, India.
Praveen C
PC
Verma
Department of Biochemistry, Banaras Hindu University, Varanasi, India.
Sushma
S
Rathaur
Eco-auditing Group, CSIR-National Botanical Research Institute, Lucknow, India.
Nandita
N
Singh
D001149:Arsenates / Q000378:metabolism*; D001151:Arsenic / Q000378:metabolism*; D018053:Arsenites / Q000378:metabolism*; D001407:Bacillus / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D009876:Operon; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D053719:Tandem Mass Spectrometry; D014835:Volatilization
D001149:Arsenates; D018053:Arsenites; C015001:arsenite; D001151:Arsenic; C025657:arsenic acid
As tolerance; Bacillus aryabhattai; Fourier-transform infrared spectroscopy; Proteins; ars Operon