GST activity and membrane lipid saturation prevents mesotrione-induced cellular damage in Pantoea ananatis.
Callisto(®), containing the active ingredient mesotrione (2-[4-methylsulfonyl-2-nitrobenzoyl]1,3-cyclohenanedione), is a selective herbicide that controls weeds in corn crops and is a potential environmental contaminant. The objective of this work was to evaluate enzymatic and structural changes in Pantoea ananatis, a strain isolated from water, in response to exposure to this herbicide. Despite degradation of mesotrione, probably due a glutathione-S-transferase (GST) pathway in Pantoea ananatis, this herbicide induced oxidative stress by increasing hydrogen peroxide production. Thiol fragments, eventually produced after mesotrione degradation, could be involved in increased GST activity. Nevertheless, there was no peroxidation damage related to this production, as malondialdehyde (MDA) synthesis, which is due to lipid peroxidation, was highest in the controls, followed by the mesotrione- and Callisto(®)-treated cultures at log growth phase. Therefore, P. ananatis can tolerate and grow in the presence of the herbicide, probably due an efficient control of oxidative stress by a polymorphic catalase system. MDA rates depend on lipid saturation due to a pattern change to a higher level of saturation. These changes are likely related to the formation of GST-mesotrione conjugates and mesotrione degradation-specific metabolites and to the presence of cytotoxic adjuvants. These features may shift lipid membrane saturation, possibly providing a protective effect to bacteria through an increase in membrane impermeability. This response system in P. ananatis provides a novel model for bacterial herbicide tolerance and adaptation in the environment.
Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Campus Universitário de Uvaranas, Av. Carlos Cavalcanti, 4748, Ponta Grossa, Paraná, 84030-900, Brazil.
Lilian P
LP
Prione
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, São Paulo, 05508-000, Brazil.
Luiz R
LR
Olchanheski
Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Campus Universitário de Uvaranas, Av. Carlos Cavalcanti, 4748, Ponta Grossa, Paraná, 84030-900, Brazil.
Leandro D
LD
Tullio
Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Campus Universitário de Uvaranas, Av. Carlos Cavalcanti, 4748, Ponta Grossa, Paraná, 84030-900, Brazil.
Bruno C E
BCE
Santo
Departamento de Enfermagem e Saúde Pública, Universidade Estadual de Ponta Grossa, Campus Universitário de Uvaranas, Av. Carlos Cavalcanti, 4748, Ponta Grossa, Paraná, 84030-900, Brazil.
Péricles M
PM
Reche
Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, São Paulo, Brazil.
Paula F
PF
Martins
Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, São Paulo, Brazil.
Giselle
G
Carvalho
Departamento de Engenharia de Alimentos, Universidade Estadual de Ponta Grossa, UEPG, Av. Carlos Cavalcanti, 4748, Ponta Grossa, PR, 84030-900, Brazil.
Ivo M
IM
Demiate
Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Campus Universitário de Uvaranas, Av. Carlos Cavalcanti, 4748, Ponta Grossa, Paraná, 84030-900, Brazil.
Sônia A V
SAV
Pileggi
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, São Paulo, 05508-000, Brazil.
Manuella N
MN
Dourado
Universidade Tecnológica Federal do Paraná, UTFPR, Campus Ponta Grossa, Av. Monteiro Lobato, Ponta Grossa, PR, 84016-210, Brazil.
Rosilene A
RA
Prestes
Department of Soil, Water, and Climate, and The BioTechnology Institute, University of Minnesota, Saint Paul, MN, 55108, USA.
Michael J
MJ
Sadowsky
Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, São Paulo, Brazil.
Ricardo A
RA
Azevedo
Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Campus Universitário de Uvaranas, Av. Carlos Cavalcanti, 4748, Ponta Grossa, Paraná, 84030-900, Brazil. pileggimarcos@gmail.com.
Marcos
http://orcid.org/0000-0003-1633-8295
M
Pileggi
Fatty acid saturation; Glutathione-S-transferase; Herbicide degradation; Lipid peroxidation; Mesotrione