A Disposable Microfluidic Device with a Screen Printed Electrode for Mimicking Phase II Metabolism.
Human metabolism is investigated using several in vitro methods. However, the current methodologies are often expensive, tedious and complicated. Over the last decade, the combination of electrochemistry (EC) with mass spectrometry (MS) has a simpler and a cheaper alternative to mimic the human metabolism. This paper describes the development of a disposable microfluidic device with a screen-printed electrode (SPE) for monitoring phase II GSH reactions. The proposed chip has the potential to be used as a primary screening tool, thus complementing the current in vitro methods.
Department of Chemistry, University of Hull, Cottingham Road, Hull HU6 7RX, UK. R.Vasiliadou@hull.ac.uk.
Rafaela
R
Vasiliadou
Department of Engineering, University of Hull, Cottingham Road, Hull HU6 7RX, UK. m.m.n.esfahani@gmail.com.
Mohammad Mehdi
MM
Nasr Esfahani
Department of Engineering, University of Hull, Cottingham Road, Hull HU6 7RX, UK. N.J.Brown@hull.ac.uk.
Nathan J
NJ
Brown
Department of Chemistry, University of Hull, Cottingham Road, Hull HU6 7RX, UK. k.j.welham@hull.ac.uk.
Kevin J
KJ
Welham
D004295:Dihydroxyphenylalanine / Q000737:chemistry / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D004572:Electrolysis; D005978:Glutathione / Q000378:metabolism; D013058:Mass Spectrometry; D008660:Metabolism; D044085:Microfluidics / Q000295:instrumentation*
D004295:Dihydroxyphenylalanine; D005978:Glutathione
electrochemistry; mass spectrometry; metabolism; screen-printed electrodes