Angiotensin II-Induced Apoptosis of Human Umbilical Vein Endothelial Cells was Inhibited by Blueberry Anthocyanin Through Bax- and Caspase 3-Dependent Pathways.
BACKGROUND This study aimed to investigate the inhibitory effect of blueberry anthocyanin (BBA) on Angiotensin II (Ang II)-induced apoptosis of human umbilical vein endothelial cells (HUVECs), and its regulation mechanisms involving Bax and Caspase 3. MATERIAL AND METHODS HUVECs were first treated by different concentrations of Ang II (10-9, 10-8, 10-7, 10-6, 10-5, and 10-4 mol/L) and BBA (80, 40, 20, 10, 5, and 2.5 μg/ml). After 24 h and 48 h of treatment, MTT was performed to detect the viability of HUVECs. Then, HUVECs were randomly divided into the Ang II group (10-6 mol/L Ang II) and Ang II + BBA group (10-6 mol/L Ang II and 20 μg/ml BBA), and the apoptosis rate was detected by flow cytometry. Western blot analysis was performed to detect the expression of Bax and Caspase 3 in these 2 groups. During the whole process, HUVECs without any treatments served as the control group. RESULTS The cell viability of HUVECs was significantly reduced by Ang II in a time- and concentration-dependent manner (P<0.05), while BBA significantly elevated the cell viability of HUVECs until a peak of 20.0 μg/ml. The apoptosis rate of HUVECs was significantly increased by Ang II (P<0.01) and reduced by the BBA intervention (P<0.05). Ang II significantly elevated the expression of Bax and Caspase 3 in HUVECs, but their expression was significantly inhibited by BBA. CONCLUSIONS BBA increased cell viability and reduced apoptosis rate of HUVECs induced by Ang II through Bax- and Caspase 3-dependent pathways.
Department of Geriatrics, The First Hospital of Jilin University, Changchun, Jilin, China (mainland).
Jian
J
Du
Department of Geriatrics, The First Hospital of Jilin University, Changchun, Jilin, China (mainland).
Jiyan
J
Leng
Department of Geriatrics, The First Hospital of Jilin University, Changchun, Jilin, China (mainland).
Li
L
Zhang
, China Jilin Forest Industry Group, Changchun, Jilin, China (mainland).
Guangxin
G
Bai
, China Jilin Forest Industry Group, Changchun, Jilin, China (mainland).
Di
D
Yang
, China Jilin Forest Industry Group, Changchun, Jilin, China (mainland).
Huan
H
Lin
Department of Geriatrics, The First Hospital of Jilin University, Changchun, Jilin, China (mainland).
Junjie
J
Qin
D000804:Angiotensin II / Q000633:toxicity*; D000872:Anthocyanins / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D029798:Blueberry Plants / Q000737:chemistry; D053148:Caspase 3 / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
D000872:Anthocyanins; C494826:BAX protein, human; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D000804:Angiotensin II; D052250:Nitric Oxide Synthase Type III; D053148:Caspase 3