Zinc supplementation imparts tolerance to arsenite stress in Hydrilla verticillata (L.f.) Royle.
The present study was aimed to analyze the effects of external Zn supply on arsenic (As) toxicity in Hydrilla verticillata (L.f.) Royle. The plants were exposed to arsenite (AsIII; 10 μM) with or without 50 and 100 μM Zn. The level of As accumulation (μg g-1dw) after 2 and 4 days was not significantly affected by Zn supply. The plants showed a significant stimulation of the thiol metabolism (nonprotein thiols, cysteine, glutathione-S-transferase activity) upon As(III) exposure in the presence of Zn as compared to As(III) alone treatment. Besides, they did not experience significant toxicity, measured in terms of hydrogen peroxide and malondialdehyde accumulation, which are the indicators of oxidative stress. The minus Zn plants suffered from oxidative stress probably due to insufficient increase in thiols to counteract the stress. Stress amelioration by Zn supply was also evident from antioxidant enzyme activities, which came close to control levels with increasing Zn supply as compared to the increase observed in As(III) alone treatment. Variable Zn supply also modulated the level of photosynthetic pigments and restored them to control levels. In conclusion, an improved supply of Zn to plants was found to augment their ability to withstand As toxicity through enhanced thiol metabolism.
a Institute of Environment and Sustainable Development, Banaras Hindu University , Varanasi , UP , India.
Sudhakar
S
Srivastava
b Centre for Environment Science and Climate Resilient Agriculture (CESCRA), Nuclear Research Laboratory (NRL), Indian Agricultural Research Institute , New Delhi , India.
Manoj
M
Shrivastava
D018053:Arsenites / Q000633:toxicity*; D001673:Biodegradation, Environmental; D031321:Hydrocharitaceae / Q000187:drug effects* / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015032:Zinc / Q000008:administration & dosage / Q000378:metabolism*
D018053:Arsenites; D014874:Water Pollutants, Chemical; D015032:Zinc; C015001:arsenite
antioxidant enzymes; arsenic; cysteine; oxidative stress; zinc