Distribution of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) in canines after intracerebroventricular injection.
In this study, we investigated the distribution of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) administered via intracerebroventricular (ICV) injection in a canine model. Ten beagles (11-13 kg per beagle) each received an injection of 1 × 106cells into the right lateral ventricle and were sacrificed 7 days after administration. Based on immunohistochemical analysis, hUCB-MSCs were observed in the brain parenchyma, especially along the lateral ventricular walls. Detected as far as 3.5 mm from the cortical surface, these cells migrated from the lateral ventricle toward the cortex. We also observed hUCB-MSCs in the hippocampus and the cervical spinal cord. According to real-time polymerase chain reaction results, most of the hUCB-MSCs were found distributed in the brain and the cervical spinal cord but not in the lungs, heart, kidneys, spleen, and liver. ICV administered hUCB-MSCs also enhanced the endogenous neural stem cell population in the subventricular zone. These results highlighted the ICV delivery route as an optimal route to be performed in stem cell-based clinical therapies for neurodegenerative diseases.
Samsung Advanced Institute for Health Science and Technology, Sungkyunkwan University, Seoul, Republic of Korea; Department of Neurology, Samsung Medical Center, Seoul, Republic of Korea; Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea; Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea; Shanghai Cuvcell Biosciences Co, Ltd, Shanghai, China.
Sang Eon
SE
Park
Department of Neurology, Samsung Medical Center, Seoul, Republic of Korea; Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea; Department of Neurology, Pusan National University Yangsan Hospital, Pusan National University School of Medicine and Medical Research Institute, Yangsan, Republic of Korea.
Na-Yeon
NY
Jung
Samsung Advanced Institute for Health Science and Technology, Sungkyunkwan University, Seoul, Republic of Korea; Department of Neurology, Samsung Medical Center, Seoul, Republic of Korea; Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea; Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea.
Na Kyung
NK
Lee
Samsung Advanced Institute for Health Science and Technology, Sungkyunkwan University, Seoul, Republic of Korea; Department of Neurology, Samsung Medical Center, Seoul, Republic of Korea; Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea; Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea.
Jeongmin
J
Lee
McGill University, Montreal, Canada.
Brian
B
Hyung
Samsung Advanced Institute for Health Science and Technology, Sungkyunkwan University, Seoul, Republic of Korea; Department of Neurology, Samsung Medical Center, Seoul, Republic of Korea; Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea; Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea.
Su Hyeon
SH
Myeong
Samsung Advanced Institute for Health Science and Technology, Sungkyunkwan University, Seoul, Republic of Korea; Department of Neurology, Samsung Medical Center, Seoul, Republic of Korea; Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea; Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea.
Hyeong Seop
HS
Kim
Department of Pathology and Translational Genomics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
Yeon-Lim
YL
Suh
Department of Neurosurgery, Samsung Medical Center, Seoul, Republic of Korea.
Jung-Il
JI
Lee
Department of Neurosurgery, Samsung Medical Center, Seoul, Republic of Korea.
Kyung Rae
KR
Cho
Korea Animal Medical Science Institute, Guri-si, Gyeonggi-do, Republic of Korea.
Do Hyung
DH
Kim
Biomedical Research Institute, MEDIPOST Co, Ltd, Gyeonggi-do, Republic of Korea.
Soo Jin
SJ
Choi
Samsung Advanced Institute for Health Science and Technology, Sungkyunkwan University, Seoul, Republic of Korea; Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea. Electronic address: jongwook.chang@samsung.com.
Jong Wook
JW
Chang
Samsung Advanced Institute for Health Science and Technology, Sungkyunkwan University, Seoul, Republic of Korea; Department of Neurology, Samsung Medical Center, Seoul, Republic of Korea; Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea. Electronic address: dukna@skku.edu.
Duk L
DL
Na
D000818:Animals; D002465:Cell Movement; D064987:Cell- and Tissue-Based Therapy; D002540:Cerebral Cortex / Q000166:cytology*; D002552:Cerebral Ventricles / Q000166:cytology*; D002574:Cervical Vertebrae; D004285:Dogs; D005312:Fetal Blood / Q000166:cytology*; D006624:Hippocampus / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells; D058953:Neural Stem Cells; D019636:Neurodegenerative Diseases / Q000628:therapy; D000072356:Parenchymal Tissue / Q000166:cytology*; D013116:Spinal Cord / Q000166:cytology*
Canine model; Distribution; Intracerebroventricular; hUCB-MSCs