Inhibition of miR-196a affects esophageal cancer cell growth in vitro.
Esophageal cancer (EC) is one of the most common causes of cancer-related mortality worldwide. Several oncogenes such as miR-196a have been implicated in the carcinogenesis and progression of EC. The purpose of this study was to determine the effects of anti-miR-196a on the growth and survival of human EC cells in vitro. We found that miR-196a was highly expressed in both TE1 and EC109 cells and that miR-196a knockdown inhibited cell proliferation and migration. Furthermore, miR-196a knockdown sensitized EC cells to radiation treatment and chemotherapy. Inhibition of miR-196a also altered cell cycle progression and induced G2/M arrest, which was related to changes in the levels of cyclin B1. ABCG2, which was highly expressed in untransfected EC cells, was inhibited by miR-196a knockdown. Thus, our results confirm the fact that miR-196a is highly involved in the biological behavior of EC progression in vitro. We conclude that miR-196a is a useful biological marker and potential therapeutic target for the clinical treatment of human EC.
Department of Oncology, The 2(nd) Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710032, China.
Yinan
Y
Ma
Department of Oncology, The 2(nd) Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710032, China.
Baofeng
B
Wang
Department of Oncology, The 2(nd) Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710032, China.
Ya
Y
Guo
Department of Oncology, The 2(nd) Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710032, China.
Yang
Y
Zhang
Department of Oncology, The 2(nd) Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710032, China.
Shan
S
Huang
Department of Oncology, The 2(nd) Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710032, China.
Xing
X
Bao
Department of Oncology, The 2(nd) Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710032, China. Electronic address: baimh2004@163.com.
Minghua
M
Bai
D014408:Biomarkers, Tumor / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques / Q000379:methods; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000235:genetics
D014408:Biomarkers, Tumor; C542543:MIRN196 microRNA, human; D035683:MicroRNAs
Chemoresistant; Esophageal cancer; Gene therapy; Radioresistant; miR-196a