A new s-adenosylhomocysteine hydrolase-linked method for adenosine detection based on DNA-templated fluorescent Cu/Ag nanoclusters.
We herein describe a novel fluorescent method for the rapid and selective detection of adenosine by utilizing DNA-templated Cu/Ag nanoclusters (NCs) and employing s-adenosylhomocysteine hydrolase (SAHH). SAHH is allowed to promote hydrolysis reaction of s-adenosylhomocysteine (SAH) and consequently produces homocysteine, which would quench the fluorescence signal from DNA-templated Cu/Ag nanoclusters employed as a signaling probe in this study. On the other hand, adenosine significantly inhibits the hydrolysis reaction and prevent the formation of homocysteine. Consequently, highly enhanced fluorescence signal from DNA-Cu/Ag NCs is retained, which could be used to identify the presence of adenosine. By employing this design principle, adenosine was sensitively detected down to 19nM with high specificity over other adenosine analogs such as AMP, ADP, ATP, cAMP, guanosine, cytidine, and urine. Finally, the diagnostic capability of this method was successfully verified by reliably detecting adenosine present in a real human serum sample.
Department of Chemical and Biomolecular Engineering (BK21+Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Jun Ki
JK
Ahn
Department of Chemical and Biomolecular Engineering (BK21+Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Hyo Yong
HY
Kim
Department of Chemical and Biomolecular Engineering (BK21+Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Songyi
S
Baek
Department of Chemical and Biomolecular Engineering (BK21+Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: hgpark@kaist.ac.kr.
Hyun Gyu
HG
Park
D000241:Adenosine / Q000737:chemistry / Q000302:isolation & purification*; D043383:Adenosylhomocysteinase / Q000737:chemistry*; D015374:Biosensing Techniques; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D005453:Fluorescence; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D012435:S-Adenosylhomocysteine; D012834:Silver / Q000737:chemistry
D012834:Silver; D003300:Copper; D004247:DNA; D012435:S-Adenosylhomocysteine; D043383:Adenosylhomocysteinase; D000241:Adenosine
Adenosine; Biosensor; Copper; Nanocluster; Silver; s-adenosylhomocysteine hydrolase