Xeno-Free Cryopreservation of Bone Marrow-Derived Multipotent Stromal Cells from Callithrix jacchus.
In the previous decade, numerous biobanks were established and have created large markets for the storage of bioactive compounds, cells, and tissues for medical and diagnostic applications. For in vivo clinical and therapeutic purposes, it is critical to use well-defined and xeno-free components during cultivation, preservation, and transplantation of biological material. Safe and efficacious storage of bioactive molecules, cells, and tissues, without the addition of undefined medium components, minimizes risks of zoonotic disease transmission and is thus an essential and desirable prerequisite for biobanks. This gives rise to a need for well-characterized and serum-free freezing media for application in cryopreservation. For this purpose, cryobiological additives such as methylcellulose, poloxamer-188, and α-tocopherol, which have previously been shown to exhibit a cytoprotective activity, have been investigated for cryoprotection on stem cells. With this strategy, the application of fetal bovine serum (FBS) could be avoided and the concentration of toxic cryoprotective agents such as dimethyl sulfoxide (DMSO) could be reduced. Our results suggest that the viability, as well as the adipogenic and osteogenic differentiation capacity of the thawed bone marrow-derived multipotent stromal stem cells, could be maintained using a freezing medium without FBS consisting of methylcellulose, poloxamer, and α-tocopherol with only 2.5% DMSO (% v/v).
Institute for Multiphase Processes, Leibniz Universität Hannover , Hannover, Germany .
Lothar
L
Lauterboeck
Institute for Multiphase Processes, Leibniz Universität Hannover , Hannover, Germany .
Debapriya
D
Saha
Institute for Multiphase Processes, Leibniz Universität Hannover , Hannover, Germany .
Anamika
A
Chatterjee
Institute for Multiphase Processes, Leibniz Universität Hannover , Hannover, Germany .
Nicola
N
Hofmann
Institute for Multiphase Processes, Leibniz Universität Hannover , Hannover, Germany .
Birgit
B
Glasmacher
D000818:Animals; D002144:Callithrix; D002454:Cell Differentiation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014021:Tissue Preservation
D003451:Cryoprotective Agents; D016895:Culture Media, Serum-Free
cryopreservation; defined cryoprotective medium; multipotent stromal cells; reduced DMSO content; serum free