Data on endogenous chicken sperm peptides and small proteins obtained through Top-Down High Resolution Mass Spectrometry.
The endogenous peptides and small proteins present in chicken sperm were identified in the context of the characterization of a fertility-diagnostic method based on the use of ICM-MS (Intact Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry). The interpretation and description of these data can be found in a research article, "Intact cell MALDI-TOF MS on sperm: a molecular test for male fertility diagnosis" (Soler et al., 2016) [1], and raw data derived from this analysis have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002768. Here, we describe the inventory of all the molecular species identified, along with their biochemical features and functional analysis. This peptide/protein catalogue can be further employed as reference for other studies and reveal that the use of proteomics allows for a global evaluation of sperm cells functions.
PRC, INRA, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France.
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Soler
PRC, INRA, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France; INRA, Plateforme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, 37380 Nouzilly, France.
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Labas
PRC, INRA, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France.
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Thélie
ISP, INRA, Université de Tours, 37380 Nouzilly, France; INRA, Plateforme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, 37380 Nouzilly, France.
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Teixeira-Gomes
PRC, INRA, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France.
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Grasseau
ISP, INRA, Université de Tours, 37380 Nouzilly, France; INRA, Plateforme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, 37380 Nouzilly, France.
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Bouguereau
PRC, INRA, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France.
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Blesbois
Chicken; Peptidome; Sperm; Top-Down HRMS