Benzimidazole resistance in the ovine Haemonchus contortus from southern Poland - coproscopical and molecular findings.
Anthelmintic resistance within nematodes has become a very common issue, however, the data about its occurrence in the gastrointestinal nematodes of sheep in Poland are very limited. This study was carried out in order to evaluate the presence of benzimidazole resistance in highly pathogenic Haemonchus contortus by means of parasitological and molecular techniques. The research represents the first Polish attempt to confirm the presence of a mutation at codon 200 of the β-tubulin isotype 1 gene in H. contortus by PCR-RFLP. The occurrence of this mutation indicates the risk of ineffective benzimidazole treatment, nowadays commonly used for parasite control in Poland. The resistant parasites were detected by means of FECRT in a sheep flock (30 individuals) from southern Poland. To confirm the resistance on the molecular level, primers designed according to the sequences available in Genebank were used to detect the mutation. Unfortunately, as the PCR product was shorter than required (403 bp), further analyses are needed. The obtained results may indicate the high variability within the parasite population. Hence, it is essential to adapt the reaction conditions to our geographic strain of the nematode - and further analyses are required.


BACKGROUND
Haemonchus contortus, benzimidazole resistance, PCR, Poland.
Jerzy
J
Kowal
Anna
A
Wyrobisz
Paweł
P
Nosal
Mirosław
M
Kucharski
Urszula
U
Kaczor
Marta
M
Skalska
Piotr
P
Sendor
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D004351:Drug Resistance; D005243:Feces / Q000469:parasitology; D006188:Haemonchiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000187:drug effects* / Q000235:genetics; D009154:Mutation; D010270:Parasite Egg Count; D011044:Poland / Q000453:epidemiology; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000469:parasitology*
D000871:Anthelmintics; D001562:Benzimidazoles; C031000:benzimidazole