Legionella clemsonensis sp. nov.: a green fluorescing Legionella strain from a patient with pneumonia.
A novel Legionella species was identified based on sequencing, cellular fatty acid analysis, biochemical reactions, and biofilm characterization. Strain D5610 was originally isolated from the bronchial wash of a patient in Ohio, USA. The bacteria were gram-negative, rod-shaped, and exhibited green fluorescence under long wave UV light. Phylogenetic analysis and fatty acid composition revealed a distinct separation within the genus. The strain grows between 26-45°C and forms biofilms equivalent to L. pneumophila Philadelphia 1. These characteristics suggest that this isolate is a novel Legionella species, for which the name Legionella clemsonensis sp nov. is proposed.
Department of Biological Sciences, Life Sciences Building.
Allison
A
Palmer
Department of Biological Sciences, Life Sciences Building.
Joseph
J
Painter
Department of Biological Sciences, Life Sciences Building.
Hayley
H
Hassler
Department of Biological Sciences, Life Sciences Building.
Vincent P
VP
Richards
Clemson Light Imaging Facility, Clemson University, 190 Collings Street, Clemson, SC 29631.
Terri
T
Bruce
Centers for Disease Control, 1600 Clifton Road, Atlanta, GA 30333, USA.
Shatavia
S
Morrison
Centers for Disease Control, 1600 Clifton Road, Atlanta, GA 30333, USA.
Ellen
E
Brown
Centers for Disease Control, 1600 Clifton Road, Atlanta, GA 30333, USA.
Natalia A
NA
Kozak-Muiznieks
Centers for Disease Control, 1600 Clifton Road, Atlanta, GA 30333, USA.
Claressa
C
Lucas
Department of Biological Sciences, Life Sciences Building. tmcneal@clemson.edu.
Tamara L
TL
McNealy
D005227:Fatty Acids / Q000378:metabolism; D005798:Genes, Bacterial; D006801:Humans; D007875:Legionella / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D007876:Legionellosis / Q000382:microbiology*; D010802:Phylogeny; D018410:Pneumonia, Bacterial / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D005227:Fatty Acids