Physiology and bioprocess of single cell of Stenotrophomonas maltophilia in bioremediation of co-existed benzo[a]pyrene and copper.
Polycyclic aromatic hydrocarbons (PAHs) and heavy metals are generally present in mixtures in the e-waste dismantling areas, posing serious health risk to the local people. Bioremediation has been considered as a promising approach for PAHs and heavy metals removal. In this study, we applied flow cytometry to obtain a better understanding of membrane potential (MP), apoptosis and cell cycle of Stenotrophomonas maltophilia affected by combined pollutants of benzo[a]pyrene(BaP) and Cu(II). The results showed that BaP was the main factor damaging the cell membrane and influencing the MP. S. maltophilia could even protect against upsetting situation by encouraging early apoptosis, whereby compromised cells committed suicide, as a result, which, in turn, facilitated the metabolism of the bacteria with high-vitality. Furthermore, even if the bacterial cell division was blocked and stopped reproduction, a large number of key enzymes inside cells could still be used for degradation of BaP. The expression of protein related to the transport and metabolism of BaP, regulation of redox reactions and phosphorylation in bacterial cell during bioprocess were evident different.
College of Natural Resources and Environment of South China Agricultural University, Guangzhou 510642, China.
Chen
C
Shuona
Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education; School of Environment and Energy, South China University of Technology, Guangzhou 510006, China. Electronic address: huayin@scut.edu.cn.
Yin
Y
Hua
College of Natural Resources and Environment of South China Agricultural University, Guangzhou 510642, China.
Chang
C
Jingjing
Department of Chemistry, School of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Peng
P
Hui
Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education; School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
Dang
D
Zhi
D017209:Apoptosis / Q000187:drug effects; D001426:Bacterial Proteins / Q000096:biosynthesis; D001564:Benzo(a)pyrene / Q000378:metabolism* / Q000633:toxicity; D001673:Biodegradation, Environmental; D001711:Biotransformation; D003300:Copper / Q000378:metabolism* / Q000633:toxicity; D059029:Electronic Waste / Q000032:analysis; D004586:Electrophoresis; D004785:Environmental Pollutants / Q000378:metabolism* / Q000633:toxicity; D005434:Flow Cytometry; D008564:Membrane Potentials / Q000187:drug effects; D008962:Models, Theoretical; D020615:Stenotrophomonas maltophilia / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D001426:Bacterial Proteins; D004785:Environmental Pollutants; D001564:Benzo(a)pyrene; D003300:Copper
Benzo[a]pyrene; Cell response; Combined pollutants; Copper; Microorganism