High efficiency cell-recycle continuous sodium gluconate production by Aspergillus niger using on-line physiological parameters association analysis to regulate feed rate rationally.
In this paper, a system of cell-recycle continuous fermentation for sodium gluconate (SG) production by Aspergillus niger (A. niger) was established. Based on initial continuous fermentation result (100.0h) with constant feed rate, an automatic feedback strategy to regulate feed rate using on-line physiological parameters (OUR and DO) was proposed and applied successfully for the first time in the improved continuous fermentation (240.5h). Due to less auxiliary time, highest SG production rate (31.05±0.29gL(-1)h(-1)) and highest yield (0.984±0.067molmol(-1)), overall SG production capacity (975.8±5.8gh(-1)) in 50-L fermentor of improved continuous fermentation increased more than 300.0% compared to that of batch fermentation. Improvement of mass transfer and dispersed mycelia morphology were the two major reasons responsible for the high SG production rate. This system had been successfully applied to industrial fermentation and SG production was greatly improved.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. box 329, 130 Meilong Road, Shanghai 200237, China.
Fei
F
Lu
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. box 329, 130 Meilong Road, Shanghai 200237, China.
Chao
C
Li
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. box 329, 130 Meilong Road, Shanghai 200237, China.
Zejian
Z
Wang
Shan Dong Fuyang Biological Technology Co., Ltd, China.
Wei
W
Zhao
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. box 329, 130 Meilong Road, Shanghai 200237, China. Electronic address: juchu@ecust.edu.cn.
Ju
J
Chu
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. box 329, 130 Meilong Road, Shanghai 200237, China.
Yingping
Y
Zhuang
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. box 329, 130 Meilong Road, Shanghai 200237, China.
Siliang
S
Zhang
D001234:Aspergillus niger / Q000378:metabolism*; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D003198:Computer Simulation; D003470:Culture Media; D005285:Fermentation; D005942:Gluconates / Q000378:metabolism*; D057446:Hydrodynamics; D059027:Recycling; D012972:Sodium Hydroxide / Q000378:metabolism
D003470:Culture Media; D005942:Gluconates; D012972:Sodium Hydroxide; C030691:gluconic acid
Aspergillus niger; Continuous fermentation; Feedback control; On-line parameters; Sodium gluconate