Investigating the passage of tetramethylpyrazine-loaded liposomes across blood-brain barrier models in vitro and ex vivo.
Tetramethylpyrazine (TMP) is a natural product extracted from Rhizoma Chuanxiong. Liposomes are an excellent delivery method that is suitable for the penetration of the blood-brain barrier (BBB). TMP-loaded liposomes are suitable for the treatment of cerebral ischemic diseases. We measured the morphology, particle size, zeta-potential, entrapment efficiency, and content of TMP-loaded liposome. The electrochemical method was adopted to determine entrapment efficiency for the first time. In addition, their diffusion through BBB models, which were built not only by in vitro cell culture but also by the ex vivo mouse subcutaneous-mucous-membrane (mSMM) method, was explored. The results showed that TMP and TMP-loaded liposomes could effectively permeate both BBB models. Therefore, the study indicated that liposomes were a novel carrier that could deliver TMP across the body barrier models and then release TMP slowly.
College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230038, People's Republic of China. Electronic address: sm_shine@163.com.
Hongmei
H
Xia
College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230038, People's Republic of China.
Zhiqing
Z
Cheng
School of life science, University of Science and Technology of China, Hefei 230027, People's Republic of China.
Yongfeng
Y
Cheng
Zhaoke (Hefei) pharmaceutical Co. Ltd., Hefei 230088, People's Republic of China.
Yinxiang
Y
Xu
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004563:Electrochemistry; D005260:Female; D008081:Liposomes; D008813:Mice, Inbred ICR; D008954:Models, Biological; D009092:Mucous Membrane / Q000187:drug effects; D010316:Particle Size; D011719:Pyrazines / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D055672:Static Electricity
D008081:Liposomes; D011719:Pyrazines; C017953:tetramethylpyrazine
Blood-brain barrier (BBB) model; Electrochemistry; Entrapment efficiency; Mouse subcutaneous-mucous-membrane (mSMM); Tetramethylpyrazine (TMP)-loaded liposome