ITF promotes migration of intestinal epithelial cells through crosstalk between the ERK and JAK/STAT3 pathways.
Intestinal trefoil factor (ITF), a member of the trefoil factor family, is a "Super-protective factor" for intestinal mucosal protection. This study was designed to explore the mechanism by which ITF promotes intestinal epithelial cell migration. Intestinal epithelial cells were treated with the human ITF (hITF). Phospho-ERK, phospho-STAT3 Tyr(705), and phospho-STAT3 Ser(727) levels were detected at different time points by western blot. To assess the potential crosstalk between the ERK and JAK/STAT3 pathways, HT-29 cells were treated with the MEK-inhibitor, U0126, and phosphor-STAT3 levels were evaluated. Conversely, cells were treated with the JAK-inhibitor, AG490, and ERK-activity was evaluated. Transwell assay was performed to investigate the effect of the crosstalk on the cell motility. MMP-2 and MMP-9 transcription was analyzed by quantitative real-time PCR. E-cadherin degradation was detected by immunofluorescence. Our results indicate that hITF simultaneously activated the ERK and JAK/STAT3 pathways and a crosstalk was detected between the two pathways. hITF increased cell migration. This effect was abolished by U0126 and AG490 treatment. hITF increased MMP2 and MMP9 mRNA levels and E-cadherin degradation and U0126 and AG490 abolished this effect of hITF. In conclusion, the hITF-induced crosstalk between the ERK and JAK/STAT3 pathways is associated with intestinal epithelial cell migration.
Department of Burn Surgery, Huaihai Hospital affiliated to Xuzhou Medical College, Xuzhou, Jiangsu Province, China.|Department of Burn Surgery, No. 97 Hospital of PLA, Xuzhou, Jiangsu Province, China.
Juan
J
Le
Department of Burn Surgery, Huaihai Hospital affiliated to Xuzhou Medical College, Xuzhou, Jiangsu Province, China.|Department of Burn Surgery, No. 97 Hospital of PLA, Xuzhou, Jiangsu Province, China.
Duan Y
DY
Zhang
Department of Burn Surgery, Huaihai Hospital affiliated to Xuzhou Medical College, Xuzhou, Jiangsu Province, China.|Department of Burn Surgery, No. 97 Hospital of PLA, Xuzhou, Jiangsu Province, China.
Yong
Y
Zhao
Department of Burn Surgery, Huaihai Hospital affiliated to Xuzhou Medical College, Xuzhou, Jiangsu Province, China.|Department of Burn Surgery, No. 97 Hospital of PLA, Xuzhou, Jiangsu Province, China.
Wei
W
Qiu
Department of Burn Surgery, Huaihai Hospital affiliated to Xuzhou Medical College, Xuzhou, Jiangsu Province, China.|Department of Burn Surgery, No. 97 Hospital of PLA, Xuzhou, Jiangsu Province, China.
Peng
P
Wang
Department of Burn Surgery, Huaihai Hospital affiliated to Xuzhou Medical College, Xuzhou, Jiangsu Province, China.|Department of Burn Surgery, No. 97 Hospital of PLA, Xuzhou, Jiangsu Province, China.
Yong
Y
Sun
D015703:Antigens, CD; D002070:Butadienes / Q000494:pharmacology; D015820:Cadherins / Q000378:metabolism; D002465:Cell Movement; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D057809:HEK293 Cells; D019073:HT29 Cells; D006801:Humans; D007422:Intestines / Q000166:cytology*; D053612:Janus Kinases / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D000071165:Trefoil Factor-3 / Q000235:genetics / Q000378:metabolism*; D020032:Tyrphostins / Q000494:pharmacology
D015703:Antigens, CD; D002070:Butadienes; C513384:CDH1 protein, human; D015820:Cadherins; D004791:Enzyme Inhibitors; D009570:Nitriles; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C082495:TFF3 protein, human; D000071165:Trefoil Factor-3; D020032:Tyrphostins; C113580:U 0126; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; D053612:Janus Kinases; D048049:Extracellular Signal-Regulated MAP Kinases; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9