Interactions of ABA signaling core components (SlPYLs, SlPP2Cs, and SlSnRK2s) in tomato (Solanum lycopersicon).
Abscisic acid (ABA) regulates fruit development and ripening via its signaling. However, the exact role of ABA signaling core components in fruit have not yet been clarified. In this study, we investigated the potential interactions of tomato (Solanum lycopersicon) ABA signaling core components using yeast two-hybrid analysis, with or without ABA at different concentrations. The results showed that among 12 PYR/PYL/RCAR ABA receptors (SlPYLs), SlPYL1, SlPYL2, SlPYL4, SlPYL5, SlPYL 7, SlPYL8, SlPYL9, SlPYL10, SlPYL11, and SlPYL13 were ABA-dependent receptors, while SlPYL3 and SlPYL12 were ABA-independent receptors. Among five SlPP2Cs (type 2C protein phosphatases) and seven SlSnRK2s (subfamily 2 of SNF1-related kinases), all SlSnRK2s could interact with SlPP2C2, while SlSnRK2.8 also interacted with SlPP2C3. SlSnRK2.5 could interact with SlABF2/4 (ABA-responsive element binding factors). Expressions of SlPYL1, SlPYL2, SlPYL8, and SlPYL10 were upregulated under exogenous ABA but downregulated under nordihydroguaiaretic acid (NDGA) at the mature green stage of fruit ripening. The expressions of SlPP2C1, SlPP2C2, SlPP2C3, and SlPP2C5 were upregulated in ABA-treated fruit, but downregulated in NDGA-treated fruit at the mature green stage. The expressions of SlSnRK2.4, SlSnRK2.5, SlSnRK2.6, and SlSnRK2.7 were upregulated by ABA, but downregulated by NDGA. However, SlSnRK2.2 was down regulated by ABA. Expression of SlABF2/3/4 was enhanced by ABA but decreased by NDGA. Based on these results, we concluded that the majority of ABA receptor PYLs interact with SlPP2Cs in an ABA-dependent manner. SlPP2C2 and SlPP2C3 can interact with SlSnRK2s. SlSnRK2.5 could interact with SlABF2/4. Most ABA signaling core components respond to exogenous ABA.
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Pei
P
Chen
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Yu-Fei
YF
Sun
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Wen-Bin
WB
Kai
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Bin
B
Liang
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Yu-Shu
YS
Zhang
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Xia-Wan
XW
Zhai
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Li
L
Jiang
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Yang-Wei
YW
Du
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China. Electronic address: pleng@cau.edu.cn.
Ping
P
Leng
D000040:Abscisic Acid / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics / Q000502:physiology*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
D002352:Carrier Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D000040:Abscisic Acid; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases; D010749:Phosphoprotein Phosphatases
ABA; SlPP2C; SlPYL; SlSnRK2; Tomato fruit; Yeast two-hybrid