R-Phycoerythrin Induces SGC-7901 Apoptosis by Arresting Cell Cycle at S Phase.
R-Phycoerythrin (R-PE), one of the chemical constituents of red algae, could produce singlet oxygen upon excitation with the appropriate radiation and possibly be used in photodynamic therapy (PDT) for cancer. Documents reported that R-PE could inhibit cell proliferation in HepG2 and A549 cells, which was significative for cancer therapy. This is due to the fact that R-PE could kill cancer cells directly as well as by PDT. However, little is known about the cytotoxicity of R-PE to the SGC-7901 cell. In this study, it has been found that R-PE could inhibit SGC-7901 proliferation and induce cell apoptosis, which was achieved by arresting the SGC-7901 cell at S phase. CyclinA, CDK2 and CDC25A are proteins associated with the S phase, and it was found that R-PE could increase the expression of cyclin A protein and decrease the expression of CDK2 and CDC25A proteins. Thus, it was concluded that R-PE reduced the CDK2 protein activated through decreasing the CDC25A factor, which reduced the formation of Cyclin-CDK complex. The reduction of Cyclin-CDK complex made the SGC-7901 cells arrest at the S phase. Therefore, R-PE induced apoptosis by arresting the SGC-7901 cell at S phase was successful, which was achieved by the expression of the CDC25A protein, which reduced the CDK2 protein actived and the formation of Cyclin-CDK complex.
Department of Pharmacy, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China. thxydsy@163.com.
Huixin
H
Tan
The Institute of Materia Medica, The Research Center of Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China. sygao2002@163.com.
Shiyong
S
Gao
The Institute of Materia Medica, The Research Center of Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China. zzyilai@163.com.
Yan
Y
Zhuang
Department of Pharmacy, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China. dyhhrb@163.com.
Yanhong
Y
Dong
Department of Pharmacy, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China. guanwxydsy@163.com.
Wenhui
W
Guan
Department of Pharmacy, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China. zhangkunydsy@163.com.
Kun
K
Zhang
Department of Pharmacy, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China. xujianydsy@163.com.
Jian
J
Xu
Department of Pharmacy, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China. cuijr2016@163.com.
Jingru
J
Cui
D017209:Apoptosis / Q000187:drug effects*; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019925:Cyclin A / Q000096:biosynthesis / Q000235:genetics; D051357:Cyclin-Dependent Kinase 2 / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D010799:Phycoerythrin / Q000494:pharmacology*; D016196:S Phase / Q000187:drug effects*; D020687:cdc25 Phosphatases / Q000096:biosynthesis / Q000235:genetics
D018797:Cell Cycle Proteins; D019925:Cyclin A; D010799:Phycoerythrin; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; C517004:CDC25A protein, human; D020687:cdc25 Phosphatases
R-phycoerythrin; apoptosis; cell cycle arrest; cell cycle-related protein; human gastric cancer