Intramuscular injection of mechano growth factor E domain peptide regulated expression of memory-related sod, miR-134 and miR-125b-3p in rat hippocampus under simulated weightlessness.
OBJECTIVE
To investigate the expression of memory-related antioxidant genes and miRNAs under simulated weightlessness and the regulation of mechano growth factor (MGF) E domain, the peptide preventing nerve damage.


RESULTS
Igf-iea and mgf mRNA levels, expression of antioxidant genes sod1 and sod2 and levels of miR-134 and miR-125b-3p increased in rat hippocampus after 14 days tail suspension to simulate weightlessness which was inhibited with intramuscular injection of E domain peptide. Therefore, administration of MGF E domain peptide could reverse increased expressions of memory-related igf-iea, mgf, sod1, sod2, miR-134 and miR-125b-3p in rat hippocampus under simulated weightlessness.


CONCLUSIONS
MGF may regulate the redox state and miRNA-targeted NR-CREB signaling, and intramuscular injection may be the alternative administration because of its safety, convenience and ability to pass through the blood brain barrier.
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, 5132-23#, Beiqing Road 26#, Haidian District, Beijing, 100094, China.|Department of Health Technology Research and Development, Space Institute of Southern China (Shenzhen), Shamiao Road 4#, Pingdi Street, Longgang District, Shenzhen, 518117, China.
Hailong
H
Chen
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, 5132-23#, Beiqing Road 26#, Haidian District, Beijing, 100094, China.
Ke
K
Lv
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, 5132-23#, Beiqing Road 26#, Haidian District, Beijing, 100094, China.
Zhongquan
Z
Dai
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, 5132-23#, Beiqing Road 26#, Haidian District, Beijing, 100094, China.
Guohua
G
Ji
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, 5132-23#, Beiqing Road 26#, Haidian District, Beijing, 100094, China.
Tingmei
T
Wang
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, 5132-23#, Beiqing Road 26#, Haidian District, Beijing, 100094, China.
Yanli
Y
Wang
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, 5132-23#, Beiqing Road 26#, Haidian District, Beijing, 100094, China.
Yongliang
Y
Zhang
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, 5132-23#, Beiqing Road 26#, Haidian District, Beijing, 100094, China.
Guanghan
G
Kan
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, 5132-23#, Beiqing Road 26#, Haidian District, Beijing, 100094, China. yinghuidd@vip.sina.com.
Yinghui
Y
Li
State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, 5132-23#, Beiqing Road 26#, Haidian District, Beijing, 100094, China. linaqu@263.net.
Lina
L
Qu
D000818:Animals; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism*; D007273:Injections, Intramuscular; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D010455:Peptides / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014893:Weightlessness
C581261:MIRN125 microRNA, rat; C581995:MIRN134 microRNA, rat; D035683:MicroRNAs; D010455:Peptides; D007334:Insulin-Like Growth Factor I
Hippocampus; Intramuscular injection; Mechano growth factor; SOD; Simulated weightlessness; miRNAs