C-type lectin (MrCTL) from the giant freshwater prawn Macrobrachium rosenbergii participates in innate immunity.
C-type lectins (CTLs) play important roles in the innate immunity of invertebrates. In this study, a novel CTL with a single carbohydrate recognition domain (CRD) containing an EPN (Glu-Pro-Asn) motif was identified from the giant freshwater prawn Macrobrachium rosenbergii. This CTL was designated as MrCTL. The cDNA of MrCTL is 1788 bp with a 657 bp open reading frame that encodes a protein of 218 amino acids. The cDNA and genome sequences of MrCTL show a polymorphism that leads to MrCTL isoforms. MrCTL was highly expressed in the gills and intestine of normal prawn, and its transcription increased after Vibrio parahaemolyticus or white spot syndrome virus (WSSV) challenge. Recombinant mature MrCTL and its single CRD could agglutinate (Ca2+-dependent) and bind both Gram-positive and Gram-negative bacteria. The recombinant proteins could attach to lipopolysaccharide and peptidoglycan in a dose-dependent manner. Recombinant MrCTL could accelerate bacterial clearance. Thus, MrCTL could serve as a pattern recognition receptor involved in the innate immunity of M. rosenbergii.
Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China.
Xin
X
Huang
Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China.
Jin-Ling
JL
Feng
State Key Laboratory Breeding Base of Marine Genetic Resource, Third Institute of Oceanography, SOA, Xiamen 361005, China.
Min
M
Jin
Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China. Electronic address: renqian0402@126.com.
Qian
Q
Ren
Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China. Electronic address: njnuwang@263.net.
Wen
W
Wang
D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D007113:Immunity, Innate; D037181:Lectins, C-Type / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D033541:Palaemonidae / Q000276:immunology* / Q000382:microbiology; D010457:Peptidoglycan / Q000494:pharmacology; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014736:Vibrio parahaemolyticus / Q000502:physiology; D046848:White spot syndrome virus 1 / Q000502:physiology
D060829:Arthropod Proteins; D018076:DNA, Complementary; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D020033:Protein Isoforms; D012333:RNA, Messenger; D011994:Recombinant Proteins
C-type lectin; Innate immunity; M. rosenbergii; Pattern recognition receptor