Impaired Japanese encephalitis virus replication in p62/SQSTM1 deficient mouse embryonic fibroblasts.
The role of the autophagy adaptor protein p62/SQSTM1 in Japanese encephalitis virus (JEV) replication in mouse embryonic fibroblasts (MEFs) was investigated. Amounts of JEV RNA and E protein were significantly smaller in p62-deficient cells than wild-type cells at 24 hr post-infection (p.i.). JEV RNA quantitation and viral plaque assays showed significant reductions in viral titers in p62-deficient cell culture fluid. Our results indicate that JEV replication is impaired in p62-deficient MEFs, suggesting that p62 positively regulates JEV replication in host cells.
Division of Protein Regulation Research, Department of Life Science, Medical Research Institute, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa 920-0293, Japan. tasakit@kanazawa-med.ac.jp.
Takafumi
T
Tasaki
Department of Infectious Diseases, Kobe Institute of Health, 4-6-5, Minatojima-Nakamachi, Chuo-ku, Kobe 650-0046, Japan.
Souichi
S
Nukuzuma
Department of Life Science, Medical Research Institute, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa 920-0293, Japan.
Tsutomu
T
Takegami
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D004664:Encephalitis Virus, Japanese / Q000235:genetics*; D004672:Encephalitis, Japanese / Q000235:genetics* / Q000821:virology*; D005347:Fibroblasts / Q000378:metabolism* / Q000821:virology*; D055786:Gene Knockout Techniques; D054884:Host-Pathogen Interactions; D051379:Mice; D000071456:Sequestosome-1 Protein / Q000172:deficiency*; D010948:Viral Plaque Assay; D014779:Virus Replication
D000071456:Sequestosome-1 Protein; C101343:Sqstm1 protein, mouse
JEV; autophagy; p62/SQSTM1