Rhizoremediation of cadmium-contaminated soil associated with hydroxamate siderophores isolated from Cd-resistant plant growth-promoting Dietzia maris and Lysinibacillus strains.
In search of multitrait plant growth-promoting (PGP) inoculants, we introduced two cadmium-resistant bacterial strains, C4 (PG), C5 (WB), and their consortium C6 (PG × WB) isolated from metal-contaminated industrial waste-fed canal near West Bengal. The test isolates were biochemically characterized and screened in vitro for siderophore production. The infrared spectra revealed the hydroxamate nature of the siderophore produced. Further in green house, siderophore-based seed inoculation with selected PGP isolates exhibited stimulatory effects on seed germination (up to 85.4%), chlorophyll index (22.9 spad unit), shoot and root length (70% and 62.7%), tiller numbers (38.82%), spikelet numbers (52.2%), straw yield (62.2%), grain yield (76.1%), total dry matter of root and shoot (55.56% and 64.4%, respectively), and grain yields (76.1%) of tested wheat cultivars. The 16S rRNA sequencing identified strain PG and WB as Dietzia maris and Lysinibacillus sp. strains. Furthermore, inoculation of C6 (consortium) in both cultivar UP-2565 and KS-227 showed maximum Cd sorption capacity in roots (38.3% and 67.1%) and shoots (68.4% and 67.5%), respectively. Both the strains and their consortium showed a great potential to increase the growth and yield of wheat cultivars, which can also be utilized for rhizoremediation process.
a Department of Microbiology , G. B. Pant University of Agriculture and Technology , Pantnagar , India.|b Patanjali Herbal Research Department , Patanjali Yogpeeth, Maharshi Dayanand Gram , Haridwar , India.
Poonam
P
Gusain
c Institute of Environmental Studies, Kurukshetra University , Kurukshetra , India.
Rashmi
R
Paliwal
d Department of Environmental Sciences , G. B. Pant University of Agriculture and Technology , Pantnagar , India.
Vir
V
Singh
D000192:Actinomycetales / Q000235:genetics / Q000378:metabolism*; D001406:Bacillaceae / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002104:Cadmium / Q000378:metabolism*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017423:Sequence Analysis, RNA; D017262:Siderophores / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*; D014908:Triticum / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D017262:Siderophores; D012989:Soil Pollutants; D002104:Cadmium
Dietzia maris; Lysinibacillus; Siderophoregenesis; cadmium; plant growth–promoting bacteria; rhizoremediation