Electrochemical detection of glutathione by using thymine-rich DNA-gated switch functionalized mesoporous silica nanoparticles.
A novel electrochemical sensor for highly sensitive and selective detection of glutathione (GSH) was developed coupled thymine-Hg2+-thymine (T-Hg2+-T) coordination with mesoporous silica nanoparticles (MSN). Hg2+ions could specifically interact with thymine bases to form strong and stable T-Hg2+-T complexes. And the electroactive molecules-methylene blue (MB) was sealed in the inner pores of MSN with a unique sequential thymine (T)-rich DNA in the presence of Hg2+. After the target was added, the formed duplex DNA T-Hg2+-T complex could deform into single-stranded DNA by a competitive displacement reaction, leading to the release of MB entrapped in the MSN. Thus the target-dependent amount of released MB could be conveniently monitored with an n-dodecanethiol modified screen-printed gold electrode. Using GSH as a model target, the proposed assay showed a wide detection ranging from 1.0nM to 1.0μM with a detection limit of 0.6nM. This strategy was simple and universal for various analytes with different competitive displacement ligands, possessing a great potential for convenient biotools testing and commercial application.
College of Life Science and Technology, Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha, 410004 China; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 China. Electronic address: bionano@163.com.
Yonghong
Y
Wang
College of Life Science and Technology, Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha, 410004 China.
Lun
L
Jiang
College of Life Science and Technology, Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha, 410004 China.
Lei
L
Chu
College of Life Science and Technology, Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha, 410004 China.
Wen
W
Liu
College of Life Science and Technology, Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha, 410004 China.
Shun
S
Wu
College of Life Science and Technology, Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha, 410004 China.
Yaohui
Y
Wu
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 China.
Xiaoxiao
X
He
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 China.
Kemin
K
Wang
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D005978:Glutathione / Q000032:analysis*; D057230:Limit of Detection; D008628:Mercury / Q000737:chemistry; D008751:Methylene Blue / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013941:Thymine / Q000737:chemistry*
D004277:DNA, Single-Stranded; D012822:Silicon Dioxide; D004247:DNA; D008628:Mercury; D005978:Glutathione; D013941:Thymine; D008751:Methylene Blue
Detection; Electrochemical; Glutathione; Mesoporous silica nanoparticles; T-Hg(2+)-T