Expression of SOX11, PAX5, TTF-1 and ISL-1 in medulloblastoma.
The aim of our study was to evaluate the immunohistochemical expression of SOX11, PAX5, TTF-1 and ISL-1 in medulloblastoma (MB) to investigate their diagnostic usefulness.


METHODS
Immunohistochemical expression of PAX5 (two antibodies: Dako, DAK-Pax5; and BD, clone 24), TTF-1 (Dako, 8G7G3/1), SOX11 (CL0142; Abcam) and ISL-1 (1 H9, Abcam) was analyzed using the h-score and Remmele score in 25 cases of MB.


RESULTS
There were 18 MBs of classic and 7 of desmoplastic type. SOX11 was strongly expressed in all tumors. The expression of PAX5 was higher and more frequent in a case of DAK-Pax5 clone (25/25) than clone 24 (6/25). ISL-1 was positive in 11 (44%) and TTF-1 in 3 (12%) cases. ISL-1 expression correlated positively (p<0.001), while TTF-1 correlated negatively with the age of patients (p=0.039). PAX5 expression correlated with ISL-1 (p=0.039) and showed a trend toward higher expression in the desmoplastic subtype (p=0.069).


CONCLUSIONS
SOX11 is strongly and robustly expressed in MBs. PAX5 expression pattern differs substantially among two antibody clones. TTF-1 and ISL-1 is associated with the age of patients.
Department of Pathomorphology, Medical University of Gdansk, Poland. Electronic address: czapiewskipiotr@gumed.edu.pl.
Piotr
P
Czapiewski
Department of Pathomorphology, Medical University of Gdansk, Poland.
Adam
A
Gorczynski
Faculty of Medicine, Medical University of Gdansk, Poland.
Klaudia
K
Radecka
Faculty of Medicine, Medical University of Gdansk, Poland.
Claudia
C
Wiewiora
Department of Neuropathology, Institute of Pathology, Medical University of Graz, Graz, Austria.
Johannes
J
Haybaeck
Department of Pathology Ingolstadt, Pathologie Ingolstadt, Germany.
Patrick
P
Adam
Institute of Pathology and Neuropathology, University Hospital Tuebingen, Eberhard-Karls-University, Tuebingen, Germany.
Falko
F
Fend
Department of Molecular Pathology and Neuropathology, Medical University of Lodz, Lodz, Poland.
Magdalena
M
Zakrzewska
Department of Neurosurgery, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
Krzysztof
K
Zakrzewski
Department of Molecular Pathology and Neuropathology, Medical University of Lodz, Lodz, Poland.
Pawel P
PP
Liberski
Department of Pathomorphology, Medical University of Gdansk, Poland.
Wojciech
W
Biernat
D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis; D002528:Cerebellar Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D060850:LIM-Homeodomain Proteins / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D008527:Medulloblastoma / Q000175:diagnosis* / Q000378:metabolism; D051757:PAX5 Transcription Factor / Q000032:analysis / Q000096:biosynthesis*; D055751:SOXC Transcription Factors / Q000032:analysis / Q000096:biosynthesis*; D014157:Transcription Factors / Q000032:analysis / Q000096:biosynthesis*; D055815:Young Adult
D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D060850:LIM-Homeodomain Proteins; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; C097473:SOX11 protein, human; D055751:SOXC Transcription Factors; C504003:TTF1 protein, human; D014157:Transcription Factors; C063843:insulin gene enhancer binding protein Isl-1
ISL-1; Medulloblastoma; PAX5; SOX11; TTF-1