High-sensitivity detection of biogenic amines with multiple reaction monitoring in fish based on benzoyl chloride derivatization.
In this study an efficient and sensitive method for biogenic amines detection was established by combining rapid extraction and derivatization with multiple reaction monitoring (MRM) based on ultra high performance liquid chromatography-triple quadrupole mass spectrometry. Bead-beating disruption and extraction using 5-sulfosalicylic acid not only improved the extraction efficiency but also was environmentally friendly. Benzoyl chloride derivatization could obtain stable biogenic amine derivatives with a shorter reaction time. By combining with MRM detection mode, higher detection sensitivity for biogenic amines could be achieved. The method showed a good linearity with linear range of 3-4 orders of magnitude and regression coefficients ranging from 0.9966 to 0.9999. The limit of detection and limit of quantitation could even reach lower pg/mL level. Satisfactory recovery was obtained from 74.9% to 119.3%. And the derivatives were stable within 48h at 4°C. The method established was used to determine content of biogenic amines in different fishes at different storage conditions. The results indicated that this method was suitable for analysis of biogenic amines.
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Yanqing
Y
Fu
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Zhihui
Z
Zhou
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Yanli
Y
Li
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Xin
X
Lu
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: zhaocx@dicp.ac.cn.
Chunxia
C
Zhao
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: xugw@dicp.ac.cn.
Guowang
G
Xu
D000818:Animals; D001565:Benzoates / Q000737:chemistry*; D001679:Biogenic Amines / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005399:Fishes; D057230:Limit of Detection; D013058:Mass Spectrometry
D001565:Benzoates; D001679:Biogenic Amines; C013409:benzoyl chloride
Benzoyl chloride; Biogenic amines; Derivatization; Liquid chromatography–mass spectrometry; Multiple reaction monitoring