Identification of the Major Components of Buddleja officinalis Extract and Their Metabolites in Rat Urine by UHPLC-LTQ-Orbitrap.
Buddleja officinalis Maxim, one of the most popular herbal medicines in China, is widely prescribed for curing eye diseases for centuries. In this study, the major components of B. officinalis extract (BOE) and their metabolites in rat urine were detected and identified by ultra-high-pressure liquid chromatography coupled with linear ion trap-orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap). A total of 19 compounds, including 8 flavonoids and 11 phenylethanoid glycosides, were confirmed or tentatively identified from BOE. In vivo, 33 components, including 3 prototypes and 30 metabolies, were confirmed or tentatively identified in rat urine samples. The metabolic pathways of different types of compounds were also proposed. This study would effectively narrow the range of potentially bioactive constituents of BOE and shed light to its action mechanism.
School of Chinese Materia Medica, Beijing Univ. of Chinese Medicine, Beijing, P.R. China.
Mohan
M
Sun
School of Chinese Materia Medica, Beijing Univ. of Chinese Medicine, Beijing, P.R. China.
Zhiqiang
Z
Luo
School of Chinese Materia Medica, Beijing Univ. of Chinese Medicine, Beijing, P.R. China. liuyang@bucm.edu.cn.
Yang
Y
Liu
School of Chinese Materia Medica, Beijing Univ. of Chinese Medicine, Beijing, P.R. China.
Ruirui
R
Yang
School of Chinese Materia Medica, Beijing Univ. of Chinese Medicine, Beijing, P.R. China.
Lina
L
Lu
School of Chinese Materia Medica, Beijing Univ. of Chinese Medicine, Beijing, P.R. China.
Guohua
G
Yu
School of Chinese Materia Medica, Beijing Univ. of Chinese Medicine, Beijing, P.R. China.
Xiaoyun
X
Ma
School of Chinese Materia Medica, Beijing Univ. of Chinese Medicine, Beijing, P.R. China.
Aoxue
A
Liu
School of Chinese Materia Medica, Beijing Univ. of Chinese Medicine, Beijing, P.R. China.
Yafang
Y
Guo
Inst. of Chinese Materia Medica, China Acad. of Chinese Medical Sciences, Beijing, P.R. China. zhyzhy007@gmail.com.
Haiyu
H
Zhao
D000818:Animals; D032861:Buddleja / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006027:Glycosides / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000737:chemistry* / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; D010936:Plant Extracts
Buddleja officinalis extract; UHPLC-LTQ-Orbitrap; major compounds; metabolites