Absence of mycobacterial DNA in peripheral blood and artery specimens in patients with Takayasu arteritis.
The objective of this study was to demonstrate the presence of mycobacterial nucleic acid sequences in peripheral blood and arteries from patients with Takayasu arteritis (TA). Polymerase chain reaction was performed to detect mycobacterial DNA from three different nucleic acid sequences including the insertion sequence (IS) 6110, the 65-kDa heat shock protein gene (HSP65), and the 16S ribosomal RNA (rRNA) gene in peripheral blood from 32 TA patients and in arterial specimens from 10 TA patients. Twenty-eight HIV-negative patients with pulmonary tuberculosis prior to therapy were tested for IS6110 in peripheral blood as positive controls, and 24 blood donors were evaluated as healthy controls (HC). All TA patients were negative for the insertion sequence IS6110 and for HSP65 and 16S rRNA genes in blood samples and in arterial specimens. IS6110 sequence was found in peripheral blood from 22 (78.5 %) patients with pulmonary tuberculosis but not in HC. In conclusion, the strategy of mycobacterial-specific nucleic acid amplification in the peripheral blood and arterial specimens of TA patients was unable to lend support to the association between TA and tuberculosis long suggested in the literature.
Universidade Federal de São Paulo, São Paulo, SP, Brazil.
Evanir S
ES
Carvalho
Universidade Federal de São Paulo, São Paulo, SP, Brazil.
Alexandre W S
AW
de Souza
Universidade Federal de São Paulo, São Paulo, SP, Brazil.
Sylvia Cardoso
SC
Leão
Universidade de São Paulo, São Paulo, SP, Brazil.
Maurício
M
Levy-Neto
Instituto Adolfo Lutz, São Paulo, SP, Brazil.
Rosangela Siqueira
RS
de Oliveira
Vanderbilt University, Nashville, TN, USA.
Wonder
W
Drake
Universidade Federal de São Paulo, São Paulo, SP, Brazil.
Marcello Fabiano
MF
de Franco
Universidade de São Paulo, São Paulo, SP, Brazil.
Paulo H N
PH
Saldiva
Instituto do Coração (INCOR), São Paulo, SP, Brazil.
Paulo Sampaio
PS
Gutierrez
Universidade Federal de São Paulo, São Paulo, SP, Brazil. luis.andrade@unifesp.br.|Rheumatology Division, Universidade Federal de São Paulo, Rua Botucatu 740 3° andar, São Paulo, SP, 04023-062, Brazil. luis.andrade@unifesp.br.
Luís Eduardo C
LE
Andrade
D000293:Adolescent; D000328:Adult; D001158:Arteries / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D016022:Case-Control Studies; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013625:Takayasu Arteritis / Q000097:blood / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000453:epidemiology
D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium
DNA; Mycobacterium tuberculosis; Polymerase chain reaction; Takayasu arteritis