In vivo and in vitro inhibitory activity of an ethanolic extract of Sargassum fulvellum and its component grasshopper ketone on atopic dermatitis.
The present study investigated the effect of Sargassum fulvellum ethanol extract (SFEE) on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)-like skin lesions in BALB/c mice. The severity of skin dermatitis, production of cytokines, and total IgE content were measured, and the histopathological features were analyzed. SFEE decreased the severity of DNCB-induced dermatitis and suppressed the serum levels of total immunoglobulin E (IgE), tumor necrosis factor (TNF)-α, and interleukin (IL)-4. In addition, SFEE reduced the production of IL-4, IL-5, and IL-13 in mice splenocytes. However, the levels of IL-10 and interferon (IFN)-γ significantly increased in mice sera and splenocytes. Histological examination revealed decreased dermal thickness and infiltration by mast cells after treatment with SFEE. Furthermore, grasshopper ketone, a compound isolated from SFEE, was found to significantly decrease cytokine production in concanavalin A-stimulated splenocytes from BALB/c mice with no cytotoxicity. Taken together, these results indicate that SFEE and the isolated grasshopper ketone have an inhibitory effect on AD by regulating immune mediators and cells and may be a potential effective alternative therapy for AD.
Department of Food Science & Technology/Institute of Food Science, Pukyong National University, Busan 608-737, Republic of Korea.
Bo-Kyeong
BK
Kang
Department of Food Science & Technology/Institute of Food Science, Pukyong National University, Busan 608-737, Republic of Korea.
Min-Ji
MJ
Kim
Institute of Fisheries Sciences, Pukyong National University, Busan 619-911, Republic of Korea.
Koth-Bong-Woo-Ri
KB
Kim
Department of Food Science & Technology/Institute of Food Science, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: dhahn@pknu.ac.kr.
Dong-Hyun
DH
Ahn
D000466:Alkadienes / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D003511:Cyclohexanols / Q000494:pharmacology / Q000627:therapeutic use*; D016207:Cytokines / Q000097:blood; D003876:Dermatitis, Atopic / Q000097:blood / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D000431:Ethanol / Q000737:chemistry; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D044647:Sargassum; D012867:Skin / Q000473:pathology; D012997:Solvents / Q000737:chemistry; D013154:Spleen / Q000166:cytology
D000466:Alkadienes; D003511:Cyclohexanols; D016207:Cytokines; D008934:Mitogens; D010936:Plant Extracts; D012997:Solvents; C000589724:grasshopper ketone; D003208:Concanavalin A; D007073:Immunoglobulin E; D000431:Ethanol
Atopic dermatitis; Cytokines; Grasshopper ketone; IgE; Sargassum fulvellum