Molecular characterization and genetic diversity of bovine Kobuvirus, Brazil.
It is suggested that Bovine kobuvirus (BKV) is involved in the etiology of gastroenteric diseases especially among calves; however, this association remains unknown. This study evaluated 216 fecal samples from cattle with and without diarrhea symptoms obtained from different regions of Brazil. A 216 bp fragment of the BKV 3D gene was amplified by RT-PCR in 14.4 % (31/216) of the studied samples, and 17 samples were subjected to nucleotide sequencing. All positive samples were obtained from animals aged less than 5 months, and most of animals presented diarrhea (p < 0.05). Phylogenetic analyses showed that the obtained sequences were grouped within the genogroup 2 of BKV forming subclades specific for each Brazilian municipality sampled. In addition, the alignment of the sequences revealed differences of nucleotides between sequences from different locations. Our results indicate for the first time that there is a regional genotypic differentiation of BKV in Brazil.
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (FZEA/USP), Avenida Duque de Caxias Norte, 225, Jardim Elite, Pirassununga, São Paulo, 13635-900, Brazil. marcelo.c@usp.br.
Marcelo
M
Candido
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (FZEA/USP), Avenida Duque de Caxias Norte, 225, Jardim Elite, Pirassununga, São Paulo, 13635-900, Brazil.
Maria Cryskely Agra
MC
Batinga
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (FZEA/USP), Avenida Duque de Caxias Norte, 225, Jardim Elite, Pirassununga, São Paulo, 13635-900, Brazil.
Anna Luiza Farias
AL
Alencar
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (FZEA/USP), Avenida Duque de Caxias Norte, 225, Jardim Elite, Pirassununga, São Paulo, 13635-900, Brazil.
Sabrina Ribeiro
SR
de Almeida-Queiroz
Department of Preventive Veterinary Medicine and Animal Reproduction, São Paulo State University (UNESP), Via de Acesso Prof. Paulo Donato Castellani, Rural, Jaboticabal, São Paulo, 14884-900, Brazil.
Maria
M
da Glória Buzinaro
Department of Clinical Analysis, Alfenas Federal University (UNIFAL), Rua Gabriel Monteiro da Silva, 700, Centro, Alfenas, Minas Gerais, 37130-000, Brazil.
Márcia Cristina
MC
Livonesi
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (FZEA/USP), Avenida Duque de Caxias Norte, 225, Jardim Elite, Pirassununga, São Paulo, 13635-900, Brazil.
Andrezza Maria
AM
Fernandes
Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (FZEA/USP), Avenida Duque de Caxias Norte, 225, Jardim Elite, Pirassununga, São Paulo, 13635-900, Brazil.
Ricardo Luiz Moro
RL
de Sousa
D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology; D005814:Genes, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D052699:Kobuvirus / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000662:veterinary; D017422:Sequence Analysis, DNA
Cattle diseases; Diarrhea; Molecular epidemiology; Picornavirus