Assay of Peripheral Regulatory Vδ1 T Cells in Ankylosing Spondylitis and its Significance.
BACKGROUND Ankylosing spondylitis (AS) involves inflammation at the sacroiliac joint and spine attachment site. This study aimed to observe the ratio and function of peripheral regulatory Vδ1 T cells in AS patients to investigate their roles in AS pathogenesis. MATERIAL AND METHODS Peripheral blood mononuclear cells (PBMC) were separated by density-gradient centrifugation from AS patients and healthy controls. Flow cytometry was used to determine the ratio between Vδ1 and CD4 T cells of PBMC in AS patients and controls. Flow cytometry sorting (FCS) was used to obtain Vδ1 and naïve CD4 T cells with purity higher than 90%. CFSE staining method was used to detect the effect of Vδ1 T cells on proliferation of naïve CD4 T cells. The effect of Vδ1 T cells on secretion of IFN-γ from naïve CD4 T cells and the ability to secrete IL-10 from Vδ1 T cells were determined by flow cytometry. RESULTS AS patients had significantly lower Vδ1 T cell ratio in PBMC compared to controls (p<0.05), but their CD4 T cell ratio was significantly elevated (p<0.05). Functional assay showed suppression of naïve CD4 T cell proliferation and IFN-γ secretion by peripheral Vδ1 T cells in AS patients (p<0.01). AS patients also had lower IL-10 secreting level from peripheral derived Vδ1 T cells (p<0.01). CONCLUSIONS The immune suppression of peripheral Vδ1 T cell in AS patient increases the ratio of peripheral CD4 T cells and IFN-γ level, leading to AS pathogenesis. This immune suppression is mainly due to suppressed IL-10 secretion.
Department of Orthopedics, Qilu Hospital, Shandong University, Jinan, Shandong, China (mainland).
Hongliang
H
Wang
Department of Orthopedics, Shandong Institute of Medicine and Health Information, Jinan, Shandong, China (mainland).
Na
N
Sun
Department of Orthopedics, Qilu Hospital, Shandong University, Jinan, Shandong, China (mainland).
Ka
K
Li
Department of Emergency, Qilu Hospital, Shandong University, Jinan, Shandong, China (mainland).
Jiguang
J
Tian
Department of Orthopedics, Qilu Hospital, Shandong University, Jinan, Shandong, China (mainland).
Jianmin
J
Li
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D013167:Spondylitis, Ankylosing / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D055815:Young Adult
D016753:Interleukin-10; D007371:Interferon-gamma