Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells.
The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with regard to developmental origin and purported function. Lineage tracing shows that they originate in Myf5(+), Pax3/Pax7(+) cell populations. Skin and muscle wounding increased PC myofiber turnover, with the satellite cell progeny being involved in muscle regeneration but with no detectable contribution to the wound-bed myofibroblasts. Since hematopoietic stem cells fuse to PC myofibers in the absence of injury, we also studied the contribution of bone marrow-derived cells to the PC satellite cell compartment, demonstrating that cells of donor origin are capable of repopulating the PC muscle stem cell niche after irradiation and bone marrow transplantation but may not fully acquire the relevant myogenic commitment.
Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, San Sebastián 20014, Spain; Neuroscience Area, Instituto Biodonostia, San Sebastián 20014, Spain; CIBERNED, Instituto de Salud Carlos III, Madrid 28029, Spain.
Neia
N
Naldaiz-Gastesi
Neuroscience Area, Instituto Biodonostia, San Sebastián 20014, Spain; CIBERNED, Instituto de Salud Carlos III, Madrid 28029, Spain.
María
M
Goicoechea
INSERM U955-E10, Université Paris Est, Faculté de Médicine, IMRB U955-E10, Creteil 94000, France.
Sonia
S
Alonso-Martín
Neuroscience Area, Instituto Biodonostia, San Sebastián 20014, Spain; CIBERNED, Instituto de Salud Carlos III, Madrid 28029, Spain.
Ana
A
Aiastui
Molecular Embryology Team, Centro Andaluz de Biología del Desarrollo, Sevilla 41013, Spain.
Macarena
M
López-Mayorga
CIBERNED, Instituto de Salud Carlos III, Madrid 28029, Spain; Laboratorio de Neurobiología Comparada, Instituto Cavanilles, Universidad de Valencia, Valencia 46980, Spain.
Paula
P
García-Belda
Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, San Sebastián 20014, Spain; Neuroscience Area, Instituto Biodonostia, San Sebastián 20014, Spain; Faculty of Medicine and Nursing, UPV-EHU, San Sebastián 20014, Spain.
Jaione
J
Lacalle
Animal Facility and Experimental Surgery, Instituto Biodonostia, San Sebastián 20014, Spain.
Carlos
C
San José
Computational Biology and Systems Biomedicine, Instituto Biodonostia, San Sebastián 20014, Spain; IKERBASQUE, Basque Foundation for Science, Bilbao 48013, Spain.
Marcos J
MJ
Araúzo-Bravo
INSA, UPS, INP, LISBP, Université de Toulouse, 31077 Toulouse, France; INRA, UMR792, Ingénierie des Systèmes Biologiques et des Procédés, 31400 Toulouse, France; CNRS, UMR5504, 31400 Toulouse, France.
Lidwine
L
Trouilh
INSA, UPS, INP, LISBP, Université de Toulouse, 31077 Toulouse, France; INRA, UMR792, Ingénierie des Systèmes Biologiques et des Procédés, 31400 Toulouse, France; CNRS, UMR5504, 31400 Toulouse, France.
Véronique
V
Anton-Leberre
Immunology and Oncology Department, Spanish National Center for Biotechnology (CNB-CSIC), Madrid 28049, Spain.
Diego
D
Herrero
IKERBASQUE, Basque Foundation for Science, Bilbao 48013, Spain; Cellular Oncology Group, Oncology Area, Instituto Biodonostia, San Sebastián 20014, Spain.
Ander
A
Matheu
Immunology and Oncology Department, Spanish National Center for Biotechnology (CNB-CSIC), Madrid 28049, Spain.
Antonio
A
Bernad
CIBERNED, Instituto de Salud Carlos III, Madrid 28029, Spain; Laboratorio de Neurobiología Comparada, Instituto Cavanilles, Universidad de Valencia, Valencia 46980, Spain.
José Manuel
JM
García-Verdugo
Molecular Embryology Team, Centro Andaluz de Biología del Desarrollo, Sevilla 41013, Spain.
Jaime J
JJ
Carvajal
INSERM U955-E10, Université Paris Est, Faculté de Médicine, IMRB U955-E10, Creteil 94000, France.
Frédéric
F
Relaix
Neuroscience Area, Instituto Biodonostia, San Sebastián 20014, Spain; CIBERNED, Instituto de Salud Carlos III, Madrid 28029, Spain; Faculty of Medicine and Nursing, Department of Neurosciences, UPV-EHU, San Sebastián 20014, Spain; Department of Neurology, Hospital Universitario Donostia, San Sebastián 20014, Spain.
Adolfo
A
Lopez de Munain
Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, San Sebastián 20014, Spain; Neuroscience Area, Instituto Biodonostia, San Sebastián 20014, Spain; CIBERNED, Instituto de Salud Carlos III, Madrid 28029, Spain. Electronic address: pgarcia@nanogune.eu.
Patricia
P
García-Parra
Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, San Sebastián 20014, Spain; Department of Biomedical Engineering, School of Engineering, Tecnun-University of Navarra, San Sebastián 20009, Spain. Electronic address: ander.izeta@biodonostia.org.
Ander
A
Izeta
D000818:Animals; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008822:Mice, Transgenic; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000502:physiology; D000072041:PAX3 Transcription Factor / Q000235:genetics; D051763:PAX7 Transcription Factor / Q000235:genetics; D010641:Phenotype; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism* / Q000637:transplantation
D015415:Biomarkers; D000072041:PAX3 Transcription Factor; D051763:PAX7 Transcription Factor