Color Change of Phenol Red by Integrated Smart Phone Camera as a Tool for the Determination of Neurotoxic Compounds.
The use of a cell phone as a detection system is easy, simple and does not require trained personnel, which is in contrast to standard laboratory instruments. This paper deals with immobilization of acetylcholinesterase (AChE) in a gelatin matrix, and phenol red, as an indicator of AChE activity, is used in order to establish a method that is easily compatible with a camera device. AChE splits acetylcholine into choline and acetic acid, which changes the pH of a medium, resulting in a phenol red color change. The coloration changed in presence of an AChE inhibitor. Measurements were performed on 3D-printed, tube-shaped holder, and digital photography, with subsequent analysis of red-green-blue (RGB), served for assay purposes. Calibration of AChE inhibitors, tacrine and galantamine, was performed, with limit of detection equal to 1.1 nM and 1.28 µM, respectively. Interferences were also measured, resulting in a proof-of-method stability. The method was further successfully validated for the standard Ellman's assay, and verified on murine plasma samples spiked with inhibitors.
Faculty of Chemical Technology, University of Pardubice, Studentska 95, Pardubice CZ-53210, Czech Republic. st30827@student.upce.cz.
Adam
A
Kostelnik
Faculty of Chemical Technology, University of Pardubice, Studentska 95, Pardubice CZ-53210, Czech Republic. Alexander.Cegan@upce.cz.
Alexander
A
Cegan
Faculty of Military Health Sciences, University of Defense, Trebesska 1575, Hradec Kralove CZ-50001, Czech Republic. miroslav.pohanka@gmail.com.
Miroslav
M
Pohanka
acetylcholinesterase; biosensor; colorimetry; drop assay; inhibitor; phenol red; smart phone