Overexpression of miR-140 Inhibits Proliferation of Osteosarcoma Cells via Suppression of Histone Deacetylase 4.
miRNAs play a pivotal role in the development and progression of osteosarcoma (OS). Previous studies indicated that miR-140 acts as a tumor suppressor in many cancers. However, its accurate expression and exact function in OS cells remain unknown. Herein, we demonstrated the lower expression of miR-140 in 40 paired OS tissues. Restoring miR-140 expression in OS cells had a marked effect on inhibiting cell proliferation and invasion, inducing cell apoptosis in vitro, and suppressing tumor growth in vivo. Moreover, a bioinformatics prediction indicated that the histone deacetylase 4 (HDAC4) is a target gene of miR-140 and is involved in miR-140-mediated suppressive effects. In conclusion, our findings show that miR-140 acts as a tumor suppressor in OS by targeting HDAC4.
Department of Orthopedics, The First Affiliated Hospital of Nanchang University, Nanchang, P.R. China.
Qianren
Q
Xiao
Lu
L
Huang
Zhongzu
Z
Zhang
Xiang
X
Chen
Jiaquan
J
Luo
Zhanmin
Z
Zhang
Shaoqing
S
Chen
Yong
Y
Shu
Zhimin
Z
Han
Kai
K
Cao
D000818:Animals; D001859:Bone Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D006655:Histone Deacetylases / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D012516:Osteosarcoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D023041:Xenograft Model Antitumor Assays / Q000379:methods
D035683:MicroRNAs; C544302:Mirn140 microRNA, human; D012097:Repressor Proteins; C400300:HDAC4 protein, human; D006655:Histone Deacetylases