Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C. pseudotuberculosis strain.
BACKGROUND
Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CL), a chronic disease that affects goats and sheep. CL is characterized by the formation of granulomas in lymph nodes and other organs, such as the lungs and liver. Current knowledge of CL pathogenesis indicates that the induction of humoral and cellular immune responses are fundamental to disease control. The aim of this study was to evaluate the humoral and cellular immune responses in BALB/c mice inoculated with a C. pseudotuberculosis strain isolated in the state of Bahia, Brazil.


RESULTS
The lymphocyte proliferation and in vitro production of IFN-γ, IL-4, IL-10, IL-12 and nitric oxide by spleen cells stimulated with secreted and somatic antigens from the studied strain were evaluated. IgG subclasses were also analyzed. Results showed a significant increase of Th1-profile cytokines after 60 days post-inoculation, as well as an important humoral response, represented by high levels of IgG2a and IgG1 against C. pseudotuberculosis.


CONCLUSIONS
The T1 strain of C. pseudotuberculosis was shown to induce humoral and cellular immune responses in BALB/c mice, but, even at a dosage of 1x10(7) CFU, no signs of the disease were observed.
Department of Exact and Earth Sciences, State University of Bahia, Campus II, Alagoinhas, BA, CEP 48110-100, Brazil. vvale@uneb.br.|Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil. vvale@uneb.br.
Vera Lúcia Costa
VL
Vale
Department of Life Sciences, State University of Bahia, Rua Silveira Martins 2555, Cabula, Salvador, BA, CEP 41150-000, Brazil.|Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.
Marcos da Costa
Mda C
Silva
Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.
Andréia Pacheco
AP
de Souza
Department of Health, Feira de Santana State University, Avenida Transnordestina s/n, Novo Horizonte, Feira de Santana, BA, CEP 44036-900, Brazil.|Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.
Soraya Castro
SC
Trindade
Department of Biointeraction, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.
Lília Ferreira
LF
de Moura-Costa
Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.
Ellen Karla Nobre
EK
Dos Santos-Lima
Department of Biointeraction, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.|Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.
Ivana Lucia de Oliveira
IL
Nascimento
Department of Exact and Earth Sciences, State University of Bahia, Campus II, Alagoinhas, BA, CEP 48110-100, Brazil.
Hugo Saba Pereira
HS
Cardoso
Department of Exact and Earth Sciences, State University of Bahia, Campus II, Alagoinhas, BA, CEP 48110-100, Brazil.
Edson de Jesus
Ede J
Marques
Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.
Bruno Jean Adrien
BJ
Paule
Department of Biointeraction, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.|Immunology and Molecular Biology Laboratory, Health Sciences Institute, Federal University of Bahia, Av. Reitor Miguel Calmon s/n, Vale do Canela, Salvador, BA, CEP 40110-100, Brazil.
Roberto José Meyer
RJ
Nascimento
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002478:Cells, Cultured; D003354:Corynebacterium Infections / Q000382:microbiology / Q000517:prevention & control*; D016925:Corynebacterium pseudotuberculosis / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D016207:Cytokines
BALB/c; Corynebacterium pseudotuberculosis; Cytokines; IgG isotypes