The significance of the increased expression of phosphorylated MeCP2 in the membranes from patients with proliferative diabetic retinopathy.
The purpose of this study was to evaluate the correlation of expression of phosphorylated methyl-CpG binding protein 2-Ser421 (MeCP2-S421) and VEGF in the membranes of patients with PDR. We examined the expression of phospho-MeCP2-S80, S421, VEGF and PEDF in surgically excised PDR membranes from 33 patients with diabetes, and idiopathic epiretinal membranes from 11 patients without diabetes, using immunohistochemistry and western blot. The colocalization of MeCP2-S421 with VEGF, PEDF, CD31, GFAP and αSMA was revealed by fluorescent double labeling. The effect of CoCl2 and knock down MeCP2 using specific siRNA on the expression of MeCP2 and VEGF were analyzed in HUCAC cells by Western blot. We found that phospho-MeCP2-S421 was significantly increased in the membranes from the patients with PDR compared with the specimens from patients without diabetes (P < 0.01). The expression of phospho-MeCP2-S421 was much stronger than that of phospho-MeCP2-S80 in the PDR membranes. Double labeling showed that the high phospho-MeCP2-S421 expression was associated with strong expression of VEGF, but not PEDF. Further, phospho-MeCP2-S421 and VEGF were increased by the stimulation of CoCl2 and knock down MeCP2 inhibited the expression of VEGF. Our result suggests that phospho-MeCP2-S421 might involve in the pathogenesis of PDR.
Henan eye Institute, Henan key laboratory of keratopathy, Zhengzhou, China.|Henan Eye Hospital, Zhengzhou, China.|Department of Ophthalmology, Henan Provincial People's hospital, Zhengzhou, China.|Department of Ophthalmology, Zhengzhou University People's hospital, Zhengzhou, China.
Xiaohua
X
Li
Henan eye Institute, Henan key laboratory of keratopathy, Zhengzhou, China.|Henan Eye Hospital, Zhengzhou, China.|Department of Ophthalmology, Henan Provincial People's hospital, Zhengzhou, China.|Department of Ophthalmology, Zhengzhou University People's hospital, Zhengzhou, China.
Xiaohui
X
Liu
Henan eye Institute, Henan key laboratory of keratopathy, Zhengzhou, China.|Henan Eye Hospital, Zhengzhou, China.|Department of Ophthalmology, Henan Provincial People's hospital, Zhengzhou, China.|Department of Ophthalmology, Zhengzhou University People's hospital, Zhengzhou, China.
Haoyi
H
Guo
Henan eye Institute, Henan key laboratory of keratopathy, Zhengzhou, China.|Henan Eye Hospital, Zhengzhou, China.|Department of Ophthalmology, Henan Provincial People's hospital, Zhengzhou, China.|Department of Ophthalmology, Zhengzhou University People's hospital, Zhengzhou, China.
Zhaoxia
Z
Zhao
Henan eye Institute, Henan key laboratory of keratopathy, Zhengzhou, China.|Henan Eye Hospital, Zhengzhou, China.|Department of Ophthalmology, Henan Provincial People's hospital, Zhengzhou, China.|Department of Ophthalmology, Zhengzhou University People's hospital, Zhengzhou, China.
Yun Sui
YS
Li
Henan eye Institute, Henan key laboratory of keratopathy, Zhengzhou, China.|Henan Eye Hospital, Zhengzhou, China.|Department of Ophthalmology, Henan Provincial People's hospital, Zhengzhou, China.|Department of Ophthalmology, Zhengzhou University People's hospital, Zhengzhou, China.
Guoming
G
Chen
D000328:Adult; D000368:Aged; D002460:Cell Line; D003035:Cobalt / Q000494:pharmacology*; D003930:Diabetic Retinopathy / Q000378:metabolism* / Q000601:surgery; D019773:Epiretinal Membrane / Q000378:metabolism* / Q000601:surgery; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D051783:Methyl-CpG-Binding Protein 2 / Q000378:metabolism*; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000378:metabolism; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism; D015843:Serpins / Q000378:metabolism; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D055815:Young Adult; D000099174:Pigment Epithelium-Derived Factor
D003035:Cobalt; D005136:Eye Proteins; D051783:Methyl-CpG-Binding Protein 2; D009414:Nerve Growth Factors; D012694:Serine; D015843:Serpins; D042461:Vascular Endothelial Growth Factor A; D000099174:Pigment Epithelium-Derived Factor; C497392:MECP2 protein, human; C467484:VEGFA protein, human; C018021:cobaltous chloride