Genomic characterization of three bovine viral diarrhea virus isolates from cattle.
Three strains of the bovine viral diarrhea virus (BVDV) were isolated from cattle in Beijing, China. To investigate their genomic features, we sequenced and characterized the complete genome of each of the isolates. Each of the three virus genomes is about 12,220 bp in length, containing a 5' untranslated region (UTR), one open reading frame (ORF) encoding a 3897-amino-acid polypeptide, and a 3' UTR. The nucleotide sequence of the three isolates were 99.0 % identical to each and other shared nucleotide sequence identities of 73.4 % to 98.3 % with other BVDV-1 strains, about 70.0 % with BVDV-2 strains, about 67.0 % with BVDV-3, and less than 67.0 % with other pestiviruses. Phylogenetic analysis of the full-length genome, 3' UTR, and the Nprogene demonstrated that the three viruses were BVDV-1 isolates. This is the first report of complete genome sequences of BVDV 1d isolates from China and might have implications for vaccine development.
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, China Agricultural University, No. 2 Yuan Ming Yuan Xi Lu, Beijing, 100193, China.
Dongjie
D
Cai
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, China Agricultural University, No. 2 Yuan Ming Yuan Xi Lu, Beijing, 100193, China.
Quanjiang
Q
Song
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, China Agricultural University, No. 2 Yuan Ming Yuan Xi Lu, Beijing, 100193, China.
Jiufeng
J
Wang
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, China Agricultural University, No. 2 Yuan Ming Yuan Xi Lu, Beijing, 100193, China. zhu_yaohong@hotmail.com.
Yaohong
Y
Zhu
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D002417:Cattle; D002681:China; D016000:Cluster Analysis; D001908:Diarrhea Viruses, Bovine Viral / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral