Identification of Endocrine Disrupting Chemicals using a Virus-Based Colorimetric Sensor.
A simple and portable colorimetric sensor based on M13 bacteriophage (phage) was devised to identify a class of endocrine disrupting chemicals, including benzene, phthalate, and chlorobenzene derivatives. Arrays of structurally and genetically modified M13 bacteriophage were fabricated so as to produce a biomimetic colorimetric sensor, and color changes in the phage arrays in response to several benzene derivatives were characterized. The sensor was also used to classify phthalate and chlorobenzene derivatives as representatives of endocrine disrupting chemicals. The characteristic color patterns obtained on exposure to various benzene derivatives enabled similar chemical structures in the vapor phase to be classified. Our sensing approach based on the use of a genetically surface modified M13 bacteriophage offers a promising platform for portable, simple environmental monitors that could be extended for use in numerous application areas, including food monitoring, security monitoring, explosive risk assessment, and point of care testing.
BK21 PLUS Nano convergence Technology Division, Pusan National University, Busan, 46241, Republic of Korea.
Jong-Sik
JS
Moon
BK21 PLUS Nano convergence Technology Division, Pusan National University, Busan, 46241, Republic of Korea.|Department of Nano Fusion Technology, Pusan National University, Busan, 46241, Republic of Korea.
Yujin
Y
Lee
Research center for Energy Convergence Technology, Pusan National University, Busan, 46241, Republic of Korea.
Dong-Myeong
DM
Shin
BK21 PLUS Nano convergence Technology Division, Pusan National University, Busan, 46241, Republic of Korea.|Department of Nano Fusion Technology, Pusan National University, Busan, 46241, Republic of Korea.
Chuntae
C
Kim
BK21 PLUS Nano convergence Technology Division, Pusan National University, Busan, 46241, Republic of Korea.|Department of Nano Fusion Technology, Pusan National University, Busan, 46241, Republic of Korea.
Won-Geun
WG
Kim
Department of Applied Nanoscience, Pusan National University, Busan, 46241, Republic of Korea.
Minji
M
Park
BK21 PLUS Nano convergence Technology Division, Pusan National University, Busan, 46241, Republic of Korea.|Department of Nano Fusion Technology, Pusan National University, Busan, 46241, Republic of Korea.
Jiye
J
Han
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 46241, Republic of Korea.
Hyerin
H
Song
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 46241, Republic of Korea.
Kyujung
K
Kim
BK21 PLUS Nano convergence Technology Division, Pusan National University, Busan, 46241, Republic of Korea.|Department of Nano Fusion Technology, Pusan National University, Busan, 46241, Republic of Korea.|Department of Nanoenergy Engineering, Pusan National University, Busan, 46241, Republic of Korea.
Jin-Woo
JW
Oh
D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002722:Chlorobenzenes / Q000032:analysis / Q000737:chemistry; D003124:Colorimetry; D052244:Endocrine Disruptors / Q000032:analysis* / Q000737:chemistry; D005740:Gases / Q000737:chemistry; D010795:Phthalic Acids / Q000032:analysis / Q000737:chemistry; D025341:Principal Component Analysis
D036022:Capsid Proteins; D002722:Chlorobenzenes; D052244:Endocrine Disruptors; D005740:Gases; D010795:Phthalic Acids; C025668:coat protein, Bacteriophage M13; C032279:phthalic acid; C031294:chlorobenzene
bacteriophages; endocrine disrupting chemicals; phthalates; polychlorinated biphenyls; sensors