Study of the participation of MMP-7, EMMPRIN and cyclophilin A in the pathogenesis of periodontal disease.
OBJECTIVE
Periodontal disease is an infectious disease resulting from the immunoinflammatory response of the host to microorganisms present in the dental biofilm which causes tissue destruction. The objective of this study was to evaluate the immunohistochemical expression of matrix metalloproteinase 7 (MMP-7), extracellular matrix metalloproteinase inducer (EMMPRIN) and cyclophilin A (CypA) in periodontal disease.


METHODS
Gingival tissue samples were divided as follows: clinically healthy gingiva (n=32), biofilm-induced gingivitis (n=28), and chronic periodontitis (n=30). Histological sections of 3μm were submitted to immunoperoxidase method and undergone quantitative analysis. The results were analyzed statistically by the Mann-Whitney and Spearman correlation tests, with the level of significance set at 0.05 (α=0.05).


RESULTS
Immunopositivity for MMP-7, EMMPRIN and CypA differed significantly between the three groups, with higher percentages of staining in chronic periodontitis specimens, followed by chronic gingivitis and healthy gingiva specimens (p<0.05). Immunoexpression of CypA and MMP-7 was higher in the intense inflammatory infiltrate observed mainly in cases of periodontitis (p<0.05). CypA expression was positively correlated with MMP-7 (r=0.831; p<0.001) and EMMPRIN (r=0.289; p=0.006). In addition, there was a significant positive correlation between probing depth and expression of MMP-7 (r=0.726; p<0.001), EMMPRIN (r=0.345; p=0.001), and CypA (r=0.803; p<0.001).


CONCLUSIONS
These results suggest that MMP-7, EMMPRIN and CypA are associated with the pathogenesis and progression of periodontal disease.
Post-Graduate Program,Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Fernando José
FJ
de Oliveira Nóbrega
Post-Graduate Program,Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil. Electronic address: denisehelen2011@hotmail.com.
Denise Hélen Imaculada Pereira
DHIP
de Oliveira
Post-Graduate Program in Dentistry, State University of Paraíba, Campina Grande, PB, Brazil.
Rodrigo Gadelha
RG
Vasconcelos
Post-Graduate Program in Dentistry, State University of Paraíba, Campina Grande, PB, Brazil.
Cassiano Francisco Weege
CFW
Nonaka
Post-Graduate Program,Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Lélia Maria Guedes
LMG
Queiroz
D051926:Basigin / Q000378:metabolism*; D055113:Chronic Periodontitis / Q000378:metabolism* / Q000473:pathology*; D021984:Cyclophilin A / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism*
C497886:BSG protein, human; D051926:Basigin; D020783:Matrix Metalloproteinase 7; D021984:Cyclophilin A
Cyclophilin A; Inflammation; Matrix metalloproteinase; Periodontitis