Synthesis of d-labeled and unlabeled ethyl succinic anhydrides and application to quantitative analysis of peptides by isotope differential mass spectrometry.
Ethyl succinic anhydride and its d5-labeled version have been synthesized and applied to quantitative analysis of peptides in combination with MALDI or ESI mass spectrometry. These modifiers react with amino groups in the N-termini and lysine side chains in proteins, and therefore the combination of these modifiers was shown to be a useful tool for quantification of peptides and hence for proteomics research.
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA; Department of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA; Graduate School of Engineering, Muroran Institute of Technology, Muroran, Hokkaido 050-8585, Japan. Electronic address: sniwayama@hotmail.com.
Satomi
S
Niwayama
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA.
Masoud
M
Zabet-Moghaddam
Joint Research Laboratory of Molecular Signature Analysis, Division of Health Sciences, Osaka University Graduate School of Medicine, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan; Laboratory and Specialty Chemicals Division, Wako Pure Chemical Industries, Ltd, 3-1-2 Doshomachi, Chuo-ku, Osaka, Osaka 540-8605, Japan.
Sadamu
S
Kurono
Graduate School of Engineering, Muroran Institute of Technology, Muroran, Hokkaido 050-8585, Japan.
Pullaiah
P
Kattanguru
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA.
Aarif L
AL
Shaikh
D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D040901:Proteomics; D013387:Succinic Anhydrides / Q000138:chemical synthesis* / Q000737:chemistry
D010455:Peptides; D013387:Succinic Anhydrides
Amino-group modifiers; Proteomics; Quantitative analysis; Soft ionization mass spectrometry; Stable isotope labeling