Sensitive arginine sensing based on inner filter effect of Au nanoparticles on the fluorescence of CdTe quantum dots.
Arginine plays an important role in many biological functions, whose detection is very significant. Herein, a sensitive, simple and cost-effective fluorescent method for the detection of arginine has been developed based on the inner filter effect (IFE) of citrate-stabilized gold nanoparticles (AuNPs) on the fluorescence of thioglycolic acid-capped CdTe quantum dots (QDs). When citrate-stabilized AuNPs were mixed with thioglycolic acid-capped CdTe QDs, the fluorescence of CdTe QDs was significantly quenched by AuNPs via the IFE. With the presence of arginine, arginine could induce the aggregation and corresponding absorption spectra change of AuNPs, which then IFE-decreased fluorescence could gradually recover with increasing amounts of arginine, achieving fluorescence "turn on" sensing for arginine. The detection mechanism is clearly illustrated and various experimental conditions were also optimized. Under the optimum conditions, a decent linear relationship was obtained in the range from 16 to 121μgL-1and the limit of detection was 5.6μgL-1. And satisfactory results were achieved in arginine analysis using arginine injection, compound amino acid injection, even blood plasma as samples. Therefore, the present assay showed various merits, such as simplicity, low cost, high sensitivity and selectivity, making it promising for sensing arginine in biological samples.
Department of Environmental Science and Engineering, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
Haijian
H
Liu
Department of Environmental Science and Engineering, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
Ming
M
Li
Department of Environmental Science and Engineering, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
Linye
L
Jiang
Agro-Environmental Protection Institute, the Ministry of Agriculture, Tianjin 300191, China.
Feng
F
Shen
School of Food and Environment, Dalian University of Technology, Panjin 124221, China. Electronic address: yufenghu@dlut.edu.cn.
Yufeng
Y
Hu
Department of Environmental Science and Engineering, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China. Electronic address: renxueqin@cau.edu.cn.
Xueqin
X
Ren
D001120:Arginine / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D005453:Fluorescence; D006046:Gold / Q000737:chemistry; D006801:Humans; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D045663:Quantum Dots / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013691:Tellurium / Q000737:chemistry; D013864:Thioglycolates / Q000737:chemistry
D019187:Cadmium Compounds; D013864:Thioglycolates; D006046:Gold; C017487:2-mercaptoacetate; D001120:Arginine; D013691:Tellurium; C028337:cadmium telluride
Arginine; CdTe quantum dots; Fluorescence; Gold nanoparticles; Inner filter effect