Long noncoding RNA SPRY4-IT1 promotes malignant development of colorectal cancer by targeting epithelial-mesenchymal transition.
The clinical significance and biological functions of long noncoding RNA SPRY4 intronic transcript 1 (SPRY4-IT1) in colorectal cancer (CRC) remain largely unclear. Herein, we are the first to report that the SPRY4-IT1 was significantly upregulated in CRC tissues, serum, and cells. Higher SPRY4-IT1 expression was markedly associated with advanced Tumor Node Metastasis (TNM) stage in a cohort of 84 CRC patients. Multivariate analyses indicated that SPRY4-IT1 expression could be useful as an independent predictor for overall survival. Further in vitro experiments revealed that knockdown of SPRY4-IT1 inhibited the proliferation, migration, and invasion of CRC cells and induced cell cycle arrestment. Moreover, we confirmed that the expression of epithelial-mesenchymal transition-related genes was modulated through alteration of SPRY4-IT1 expression. These results suggest that SPRY4-IT1, as an oncogenic regulator, may serve as a candidate prognostic marker and potential target for CRC therapies.
Department of General Surgery, The People's Hospital of Putuo, Zhoushan.
Dong
D
Cao
Department of General Surgery, The People's Hospital of Putuo, Zhoushan.
Qiong
Q
Ding
Department of General Surgery, The People's Hospital of Putuo, Zhoushan.
Wubin
W
Yu
Department of General Surgery, The People's Hospital of Putuo, Zhoushan.
Ming
M
Gao
Department of Cardiology, The Second People's Hospital of Lianyungang, Xinpu, People's Republic of China.
Yilian
Y
Wang
SPRY4-IT1; colorectal cancer; long noncoding RNA; survival