Glutamine is required for snakehead fish vesiculovirus propagation via replenishing the tricarboxylic acid cycle.
Snakehead fish vesiculovirus (SHVV), a member of the family Rhabdoviridae, has caused mass mortality in snakehead fish culture in China. Previous transcriptomic sequencing of SHVV-infected and non-infected striped snakehead fish cells (SSN-1) showed that glutaminase (GLS), the critical enzyme of glutamine metabolism, was upregulated upon SHVV infection. It therefore drew our attention to investigating the role of glutamine in SHVV propagation. Glutamine deprivation significantly reduced the expression of the mRNAs and proteins of SHVV, and the production of virus particles, indicating that glutamine was required for SHVV propagation. Glutamine can be converted to glutamate by GLS, and then be converted to α-ketoglutarate, to join in the tricarboxylic acid (TCA) cycle. Addition of the TCA cycle intermediate α-ketoglutarate, oxaloacetic acid or pyruvate significantly restored SHVV propagation, indicating that the requirement of glutamine for SHVV propagation was due to its replenishment of the TCA cycle. Inhibiting the activity of GLS in SSN-1 cells by an inhibitor, bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide, decreased SHVV propagation, while overexpression of GLS increased SHVV propagation. Taken together, our data have revealed the relationship between glutamine metabolism and SHVV propagation.
Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Lindan
L
Sun
Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Lizhu
L
Yi
Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Chi
C
Zhang
Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Xiaodan
X
Liu
Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Shuangshuang
S
Feng
Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Wenjie
W
Chen
Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Jiangfeng
J
Lan
Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Lijuan
L
Zhao
Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Jiagang
J
Tu
State Key Laboratory of Marine Resource Utilization in the South China Sea, Key Laboratory of Tropical Biological Resources of the Ministry of Education, College of Marine Science, Hainan University, Haikou, Hainan 570228, PR China.|Department of Aquatic Animal Medicine, Research Center for Marine Biology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Li
L
Lin
D000818:Animals; D002460:Cell Line; D002681:China; D002952:Citric Acid Cycle; D005393:Fish Diseases / Q000378:metabolism* / Q000821:virology; D005973:Glutamine / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000378:metabolism; D010473:Perciformes / Q000378:metabolism / Q000821:virology*; D018353:Rhabdoviridae Infections / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D018116:Vesiculovirus / Q000502:physiology*; D014779:Virus Replication
D007656:Ketoglutaric Acids; D005973:Glutamine