Scattering correction through a space-variant blind deconvolution algorithm.
Scattering within biological samples limits the imaging depth and the resolution in microscopy. We present a prior and regularization approach for blind deconvolution algorithms to correct the influence of scattering to increase the imaging depth and resolution. The effect of the prior is demonstrated on a three-dimensional image stack of a zebrafish embryo captured with a selective plane illumination microscope. Blind deconvolution algorithms model the recorded image as a convolution between the distribution of fluorophores and a point spread function (PSF). Our prior uses image information from adjacent z-planes to estimate the unknown blur in tissue. The increased size of the PSF due to the cascading effect of scattering in deeper tissue is accounted for by a depth adaptive regularizer model. In a zebrafish sample, we were able to extend the point in depth, where scattering has a significant effect on the image quality by around 30???m.
Carl Zeiss AG, Corporate Research and Technology, Carl-Zeiss-Promenade 10, 07745 Jena, GermanybInstitute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum, München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
Koberstein-Schwarz
KS
Benno
Carl Zeiss AG, Corporate Research and Technology, Carl-Zeiss-Promenade 10, 07745 Jena, Germany.
Omlor
O
Lars
Carl Zeiss AG, Corporate Research and Technology, Carl-Zeiss-Promenade 10, 07745 Jena, Germany.
Schmitt-Manderbach
SM
Tobias
Carl Zeiss Vision International GmbH, Technology and Innovation, Turnstr. 27, 73430 Aalen, Germany.
Mappes
M
Timo
Institute for Biological and Medical Imaging, Technische Universität München and Helmholtz Zentrum, München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
Ntziachristos
N
Vasilis
D000465:Algorithms; D000818:Animals; D004625:Embryo, Nonmammalian / Q000000981:diagnostic imaging; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy; D012542:Scattering, Radiation; D015027:Zebrafish