Developmental toxicity of the common UV filter, benophenone-2, in zebrafish embryos.
Benozophenone (BP) type UV filters are extensively used in the personal care products to provide protection against the harmful effects of UV radiation. BPs are one of the primary components in the UV filter family, in which benophenone-2 (BP2) is widely used as a UV filter reagent in the sunscreen. Humans used these personal care products directly on skin and the chemicals will be washed away to the water system. BP2 has been identified as one of the endocrine disruptor chemicals, which can inference the synthesis, metabolism, and action of endogenous hormones. Environmentally, it has been found to contaminate water worldwide. In this study, we aimed to unfold the possible developmental toxicology of this chemical. Zebrafish are used as the screening model to perform in situ hybridization staining to investigate the effects of BP2 on segmentation, brain regionalization, and facial formation at four developmental stages (10-12 somite, prim-5, 2 and 5 days post-fertilization). Results showed 40 μM (9.85 mg L-1) or above BP2 exposure in zebrafish embryos for 5 days resulted in lipid accumulation in the yolk sac and facial malformation via affecting the lipid processing and the expression of cranial neural crest cells respectively. To conclude, the study alarmed its potential developmental toxicities at high dosage exposure.
Department of Biology, Hong Kong Baptist University, Hong Kong Special Administrative Region.
Henry C H
HC
Fong
Department of Biology, Hong Kong Baptist University, Hong Kong Special Administrative Region; Department of Biology and Chemistry, City University of Hong Kong, Hong Kong Special Administrative Region.
Jeff C H
JC
Ho
Department of Biology and Chemistry, City University of Hong Kong, Hong Kong Special Administrative Region.
Angela H Y
AH
Cheung
Department of Biology and Chemistry, City University of Hong Kong, Hong Kong Special Administrative Region.
K P
KP
Lai
Faculty of Agriculture, Kyushu University, Fukuoka, Japan. Electronic address: kftse@agr.kyushu-u.ac.jp.
William K F
WK
Tse
D000818:Animals; D001577:Benzophenones / Q000633:toxicity*; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D047108:Embryonic Development / Q000187:drug effects*; D052244:Endocrine Disruptors / Q000633:toxicity*; D013473:Sunscreening Agents / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology*
D001577:Benzophenones; D052244:Endocrine Disruptors; D013473:Sunscreening Agents; D014874:Water Pollutants, Chemical
Embryogenesis; Environmental pollutant; Facial malformation