Characteristics of two novel cold- and salt-tolerant ammonia-oxidizing bacteria from Liaohe Estuarine Wetland.
To achieve a better contaminant removal efficiency in a low-temperature and high-salt environment, two novel strains of cold- and salt-tolerant ammonia-oxidizing bacteria (AOB), i.e., Ochrobactrum sp. (HXN-1) and Aquamicrobium sp. (HXN-2), were isolated from the surface sediment of Liaohe Estuarine Wetland (LEW), China. The optimization of initial ammonia nitrogen concentration, pH, carbon-nitrogen ratio, and petroleum hydrocarbons (PHCs) to improve the ammonia-oxidation capacity of the two bacterial strains was studied. Both bacterial strains showed a high ammonia nitrogen removal rate of over 80% under a high salinity of 10‰. Even at a temperature as low as 15°C, HXN-1 and HXN-2 could achieve an ammonia nitrogen removal rate of 53% and 62%, respectively. The cold- and salt-tolerant AOB in this study demonstrated a high potential for ammonia nitrogen removal from LEW.
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Xiao
X
Huang
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; The Key Lab of Marine Environmental Science and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
Jie
J
Bai
College of Marine Life Science, Ocean University of China, Qingdao 266003, China. Electronic address: likr@ouc.edu.cn.
Kui-Ran
KR
Li
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; The Key Lab of Marine Environmental Science and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
Yang-Guo
YG
Zhao
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; The Key Lab of Marine Environmental Science and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
Wei-Jun
WJ
Tian
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Jia-Jia
JJ
Dang
D000641:Ammonia / Q000378:metabolism*; D001105:Archaea; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020562:Betaproteobacteria; D002681:China; D003080:Cold Temperature; D004784:Environmental Monitoring; D063366:Estuaries; D019015:Geologic Sediments / Q000382:microbiology; D009584:Nitrogen; D010084:Oxidation-Reduction; D010802:Phylogeny; D012965:Sodium Chloride; D053833:Wetlands
D012965:Sodium Chloride; D000641:Ammonia; D009584:Nitrogen
Ammonia oxidation characteristics; Ammonia-oxidizing bacteria; Cold tolerance; Liaohe Estuarine Wetland; Salt tolerance