Role of autophagy in cellular response to infection with Orf virus Jilin isolate.
Autophagy is a conserved catabolic process of the cell, which has been described to be involved in the development of various viral diseases. However, the role of autophagy in Orf virus (ORFV) replication remains unknown. In this study, we provide the first evidence that ORFV infection triggered autophagy in primary ovine fetal turbinate cells (OFTu) based on the appearance of abundant double- and single-membrane vesicles, the accumulation of LC3 fluorescent puncta, the enhancement of LC3-I/-II conversion, and autophagic flux. Moreover, modulation of ORFV-induced autophagy by rapamycin (RAPA), Earle's balanced salts solution (EBSS), chloroquine (CQ) or 3-methyladenime (3-MA) does not affect virus production. In conclusion, these results suggest that autophagy can be induced in host cells by ORFV infection, but which maybe not essential for ORFV replication.
College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
Yungang
Y
Lan
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
Gaili
G
Wang
College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
Deguang
D
Song
College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
Wenqi
W
He
College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
Di
D
Zhang
College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
Houshuang
H
Huang
College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
Jingying
J
Bi
College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China; Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
Feng
F
Gao
College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China. Electronic address: kuizhao@jlu.edu.cn.
Kui
K
Zhao
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001343:Autophagy; D002478:Cells, Cultured; D002738:Chloroquine / Q000494:pharmacology; D004474:Ecthyma, Contagious / Q000175:diagnosis / Q000473:pathology / Q000821:virology*; D054884:Host-Pathogen Interactions; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D009923:Orf virus / Q000502:physiology*; D012756:Sheep; D020123:Sirolimus / Q000494:pharmacology; D014779:Virus Replication
C025946:3-methyladenine; D002738:Chloroquine; D000225:Adenine; D020123:Sirolimus
Autophagic flux; Autophagy; OFTu cells; ORFV