Dynamic thiol/disulphide homeostasis before and after radical prostatectomy in patients with prostate cancer.
Thiol groups are important anti-oxidants and essential molecules protecting organism against the harmful effects of reactive oxygen species (ROS). The aim of our study is to evaluate thiol-disulphide homeostasis with a novel recent automated method in patients with localized prostate cancer (PC) before and six months after radical prostatectomy (RP). 18 patients with PC and 17 healthy control subjects were enrolled into the study. Blood samples were collected from the controls subjects and patients before and six months after RP. Thiol-disulphide homeostasis was determined using a recently developed novel method. Prostate-specific antigen (PSA), albumin, total protein, total thiol, native thiol, disulphide and total antioxidant status (TAS) were measured and compared between the groups. Native thiol, total thiol and TAS levels were significantly higher in the control group than the patients before RP (p < .001). There was a non-significant increase in the native thiol, total thiol and TAS levels in the patients six months after RP in comparison to the levels before RP (p values .3, .3 and .09, respectively). We found a significant negative correlation between PSA and thiol levels. Our study demonstrated that the decreased thiol and TAS levels weakened anti-oxidant defence mechanism in the patients with PC as indicated. Increased oxidative stress in prostate cancer patients may cause metabolic disturbance and have a role in the aetiopathogenesis of prostate cancer.
a Biochemistry Laboratory , Kemer State Hospital , Antalya , Turkey.
Ferhat
F
Hanikoglu
b Department of Biochemistry, Medical Faculty , Akdeniz University , Antalya , Turkey.
Aysegul
A
Hanikoglu
b Department of Biochemistry, Medical Faculty , Akdeniz University , Antalya , Turkey.
Ertan
E
Kucuksayan
c Department of Biochemistry, Medical Faculty , Yildirim Beyazit University , Ankara , Turkey.
Murat
M
Alisik
d Department of Urology, Medical Faculty , Akdeniz University , Antalya , Turkey.
Ahmet Anil
AA
Gocener
c Department of Biochemistry, Medical Faculty , Yildirim Beyazit University , Ankara , Turkey.
Ozcan
O
Erel
d Department of Urology, Medical Faculty , Akdeniz University , Antalya , Turkey.
Mehmet
M
Baykara
e Department of Diagnostic and Clinical Medicine and Public Health , University of Modena and Reggio Emilia , Modena, Italy.
Aurora
A
Cuoghi
e Department of Diagnostic and Clinical Medicine and Public Health , University of Modena and Reggio Emilia , Modena, Italy.
Aldo
A
Tomasi
b Department of Biochemistry, Medical Faculty , Akdeniz University , Antalya , Turkey.
Tomris
T
Ozben
D004220:Disulfides / Q000378:metabolism*; D006706:Homeostasis; D006801:Humans; D008297:Male; D011468:Prostatectomy / Q000379:methods*; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery
D004220:Disulfides
Prostate cancer; antioxidant; dynamic thiol–disulphide homeostasis; reactive oxygen species