Chlamydia pneumoniae CPj0783 interaction with Huntingtin-protein14.
Chlamydia pneumoniae is a Gram-negative, obligate intracellular pathogen that causes community-acquired respiratory infections. After C. pneumoniae invades host cells, it disturbs the vesicle transport system to escape host lysosomal or autophagosomal degradation. By using a yeast mis-sorting assay, we found 10 C. pneumoniae candidate genes involved in aberrant vesicular trafficking in host cells. One of the candidate genes, CPj0783, was recognized by antibodies from C. pneumoniae-infected patients. The expression of CPj0783 was detected at mid to late-cycle time points and increased during the inclusion maturation. Two-hybrid screening in yeast cells revealed that CPj0783 interacted with Huntingtin-interacting protein 14 (HIP14). The specific interaction between CPj0783 and HIP14 could be demonstrated by an in vivo co-immunoprecipitation assay and an in vitro GST pull-down assay. It was also demonstrated that HIP14 was localized in the Golgi apparatus and colocalized with CPj0783. HIP14 has a palmitoyl transferase activity that is involved in the palmitoylation-dependent vesicular trafficking of several acylated proteins. These findings suggest that CPj0783 might cause abnormal vesicle-mediated transport by interacting with HIP14. [Int Microbiol 18(4):225-233 (2015)].
Department of Molecular Genetics, Kawasaki Medical School, Kurashiki, Okayama, Japan.
Izumi
I
Yanatori
Department of Molecular Genetics, Kawasaki Medical School, Kurashiki, Okayama, Japan.
Yumiko
Y
Yasui
Department of Pediatrics, Kawasaki Medical School, Kurashiki, Okayama, Japan.
Kazunobu
K
Ouchi
Department of Molecular Genetics, Kawasaki Medical School, Kurashiki, Okayama, Japan.
Fumio
F
Kishi
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D023521:Chlamydophila Infections / Q000378:metabolism* / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D020798:Two-Hybrid System Techniques
D048868:Adaptor Proteins, Signal Transducing; D001426:Bacterial Proteins; D009419:Nerve Tissue Proteins; D000217:Acyltransferases; C473038:ZDHHC17 protein, human
Chlamydia pneumoniae; intracellular pathogens; protein mis-sorting; vesicle transport; yeast two-hybrid screening CPj0783–HIP14