Rapid start-up of the anammox process: Effects of five different sludge extracellular polymeric substances on the activity of anammox bacteria.
This study investigated the rapid start-up of the anaerobic ammonium oxidation (anammox) strategy by inoculating different biomass ratios of denitrifying granular sludge and anammox bacteria. The results demonstrated that two reactors (R1 and R2) were rapidly and successfully started-up on days 25 and 28, respectively, with nitrogen removal rates (NRRs) of 0.70kg/(m(3)·d) and 0.72kg/(m(3)·d) at biomass ratios of 10:1 (R1) and 50:1 (R2). The explanation for rapid start-up was found by examining the effect of five different sludge extracellular polymeric substances (EPS) on the activity of anammox bacteria in the batch experiments. Batch experiments results first demonstrated that the denitrification sludge EPS (DS-EPS) enhanced the anammox bacteria activity the most, and NO2(-)-N, NH4(+)-N removal rates were 1.88- and 1.53-fold higher than the control with optimal DS-EPS volume of 10mL. The rapid start-up strategy makes possible the application of anammox to practical engineering.
Tianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, Jinjing Road 26#, Tianjin 300384, PR China; School of Environmental Science and Engineering & Pollution Prevention Biotechnology Laboratory of Hebei Province, Hebei University of Science and Technology, Yuhua East Road 70#, Shijiazhuang 050018, PR China. Electronic address: jianbguo@163.com.
Jianbo
J
Guo
School of Environmental Science and Engineering & Pollution Prevention Biotechnology Laboratory of Hebei Province, Hebei University of Science and Technology, Yuhua East Road 70#, Shijiazhuang 050018, PR China.
Sihui
S
Wang
School of Environmental Science and Engineering & Pollution Prevention Biotechnology Laboratory of Hebei Province, Hebei University of Science and Technology, Yuhua East Road 70#, Shijiazhuang 050018, PR China.
Jing
J
Lian
Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2007, Australia.
Huu Hao
HH
Ngo
Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2007, Australia.
Wenshan
W
Guo
School of Environmental Science and Engineering & Pollution Prevention Biotechnology Laboratory of Hebei Province, Hebei University of Science and Technology, Yuhua East Road 70#, Shijiazhuang 050018, PR China.
Yunman
Y
Liu
Tianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, Jinjing Road 26#, Tianjin 300384, PR China.
Yuanyuan
Y
Song
D000641:Ammonia / Q000378:metabolism*; D000693:Anaerobiosis / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects* / Q000378:metabolism*; D001704:Biopolymers / Q000494:pharmacology*; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D058440:Denitrification / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid
D001704:Biopolymers; D012722:Sewage; D000641:Ammonia
Anammox; Anammox bacteria activity; Denitrifying granular sludge; Extracellular polymeric substances; Start-up