Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea.
Small GTPases play important roles in the growth, development and environmental responses of eukaryotes. Based on the genomic sequence of the straw mushroom Volvariella volvacea, 44 small GTPases were identified. A clustering analysis using human small GTPases as the references revealed that V. volvacea small GTPases can be grouped into five families: nine are in the Ras family, 10 are in the Rho family, 15 are in the Rab family, one is in the Ran family and nine are in the Arf family. The transcription of vvran1 was up-regulated upon hydrogen peroxide (H₂O₂) stress, and could be repressed by diphenyleneiodonium chloride (DPI), a NADPH oxidase-specific inhibitor. The number of vvran1 transcripts also increased upon cold stress. Diphenyleneiodonium chloride, but not the superoxide dismutase (SOD) inhibitor diethy dithiocarbamate (DDC), could suppress the up-regulation of vvran1 gene expression to cold stress. These results combined with the high correlations between gene expression and superoxide anion (O₂(-)) generation indicated that vvran1 could be one of the candidate genes in the downstream of O₂(-) mediated pathways that are generated by NADPH oxidase under low temperature and oxidative stresses.
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. junjie017@163.com.
Jun-Jie
JJ
Yan
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. 18705043595@163.com.
Bin
B
Xie
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. zhanglei311540@163.com.
Lei
L
Zhang
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. lisj@im.ac.cn.
Shao-Jie
SJ
Li
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. arendvanpeer@gmail.com.
Arend F
AF
van Peer
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. wutaju@163.com.
Ta-Ju
TJ
Wu
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. cbz_2006@163.com.
Bing-Zhi
BZ
Chen
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. mrcfafu@163.com.
Bao-Gui
BG
Xie
D003080:Cold Temperature; D005656:Fungal Proteins / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics*; D005810:Multigene Family; D009861:Onium Compounds / Q000494:pharmacology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D055367:Volvariella / Q000201:enzymology* / Q000235:genetics; D020931:ran GTP-Binding Protein / Q000235:genetics
D005656:Fungal Proteins; D009861:Onium Compounds; C007517:diphenyleneiodonium; D006861:Hydrogen Peroxide; D020559:Monomeric GTP-Binding Proteins; D020931:ran GTP-Binding Protein
RT-qPCR; abiotic stresses; edible fungi; small GTPase superfamily; superoxide anion