Coetzeea brasiliensis gen. nov., sp. nov. isolated from larvae of Anopheles darlingi.
A Gram-stain-negative, rod-shaped strain, Braz8T, isolated from larvae of Anopheles darlingi was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Braz8T was related most closely to species of the genus Thorsellia, with 95.6, 96.5 and 96.6 % similarity to the type strains of Thorsellia anophelis, Thorsellia kandunguensis and Thorsellia kenyensis, respectively, and formed a separate branch in the phylogenetic tree next to the monophyletic cluster of the genus Thorsellia. Chemotaxonomic data supported the allocation of the strain to the family Thorselliaceae. The major fatty acids were C18 : 1ω7c, C16 : 0 and C14 : 0. The quinone system was composed of ubiquinones Q-8 and Q-7 (1 : 0.3), the predominant polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the polyamine pattern showed the major compound putrescine. However, qualitative and quantitative differences in the major polyamine, polar lipid profile and fatty acid patterns distinguished strain Braz8T from species of the genus Thorsellia. Phylogenetic analysis based on 16S rRNA gene sequences, average nucleotide identity, DNA-DNA hybridization, multilocus sequence analysis as well as physiological and biochemical tests distinguished strain Braz8T both genotypically and phenotypically from the three Thorsellia species but also showed its placement in the family Thorselliaceae. Thus, strain Braz8T is considered to represent a novel species of a new genus most closely related to the genus Thorsellia, for which the name Coetzeea brasiliensis gen. nov., sp. nov. is proposed. The type strain of Coetzeea brasiliensis is Braz8T (=LMG 29552T=CIP 111088T).
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, IFZ-Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
Peter
P
Kämpfer
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, IFZ-Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
Stefanie P
SP
Glaeser
Department of Molecular Biology and Biochemistry, 3205 Mc-Gaugh Hall, University of California, Irvine, CA 92697, USA.
Osvaldo
O
Marinotti
Department of Medical Biochemistry and Microbiology, Uppsala University, PO Box 582, SE-751 23 Uppsala, Sweden.
Lionel
L
Guy
Department of Microbiology, Swedish University of Agricultural Sciences (SLU), PO Box 7025, SE-750 07 Uppsala, Sweden.
Sebastian
S
Håkansson
Laboratório de Malária e Dengue, Instituto Nacional de Pesquisas da Amazonia, AM 69011970 Manaus, Brazil.
Wanderli P
WP
Tadei
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Hans-Jürgen
HJ
Busse
Department of Ecology, Swedish University of Agricultural Sciences (SLU), PO Box 7044, SE-750 07 Uppsala, Sweden.
Olle
O
Terenius
D000818:Animals; D000852:Anopheles / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D007814:Larva / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011700:Putrescine / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8; D011700:Putrescine