A simple, efficient and inexpensive method for malaria parasites' DNA catching from fixed Giemsa-stained blood slides.
As parasitological or microscopic method is the gold standard and the best method for diagnosis of malaria, so fixed Geimsa-stained blood slides in the form of thick and thin blood smears are the most important data collections of malaria, especially historical slides. The parasites are dead but their DNA is valuable for many molecular biologic researches. A simple and efficient method for catching and extraction malaria parasites' DNA with a desired yield from dried and stained blood on slides is the first and major step. Introduction of an applicable, efficient and inexpensive DNA catching method and assessment of its performance in following molecular applications  was the main objective of present study.
Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran.
Abbas Ali
AA
Eskandarian
Departments of Microbiology, Faculty of Biological Sciences, Islamic Azad University, Falavarjan Branch, Isfahan, Iran.
Sara
S
Moradi
Departments of Microbiology, Faculty of Biological Sciences, Islamic Azad University, Falavarjan Branch, Isfahan, Iran.
Saeed
S
Abedi
Blood slide; DNA extraction; Fixed Giemsa-stained; Malaria; Plasmodium falciparum; Plasmodium vivax