Inhibitory effects of dietary soyasaponin on 2,4-dinitrofluorobenzene-induced contact hypersensitivity in mice.
Soyasaponins (SSs) abundant in soybean have anti-inflammatory activities; however, their therapeutic effects on allergic contact dermatitis (ACD) remain unknown. To assess the effects of SS-enriched diets on ACD, we used a mouse model of contact hypersensitivity (CHS). Mice were fed low-dose or high-dose SS-containing diets for 3 weeks prior to CHS induction with 2,4-dinitrofluorobenzene (DNFB). The low-dose SS diet attenuated DNFB-induced ear swelling and tissue oedema, and reduced the number of infiltrating Gr-1-positive myeloid cells. Low-dose, but not high-dose, SSs decreased chemokine (C-X-C motif) ligand 2 (CXCL2) and triggering receptor expressed on myeloid cells (TREM)-1 production in ear tissues, compared to a control. Taxonomic 16S rRNA analysis revealed significant alterations in faecal microbiota caused by CHS, which were reversed by low-dose SSs. The low-dose SS and non-CHS groups clustered together, while the high-dose SS group split between CHS and non-CHS clusters. Our results demonstrated that low-dose SSs alleviated CHS symptoms by attenuating inflammation and improving the intestinal microbiota composition, suggesting that dietary SSs may have beneficial effects on ACD.
Department of Clinical Nutrition, Kawasaki University of Medical Welfare, Okayama, Japan.
Takao
T
Nagano
Quality Assurance Department, Fuji Oil Holdings Inc., Osaka, Japan.
Mitsuru
M
Katase
Protein Research Group, Fuji Oil Holdings Inc., Ibaraki, Japan.
Kazunobu
K
Tsumura
Department of Microbiology, Kawasaki Medical School, Okayama, Japan.
Mineki
M
Saito
Department of Applied Molecular Bioscience, Nagoya University, Aichi, Japan.
Tsukasa
T
Matsuda
D000818:Animals; D054426:Chemokine CXCL2 / Q000378:metabolism; D003877:Dermatitis, Contact / Q000188:drug therapy* / Q000473:pathology; D019587:Dietary Supplements; D004139:Dinitrofluorobenzene; D005243:Feces / Q000382:microbiology; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D012503:Saponins / Q000008:administration & dosage / Q000627:therapeutic use*; D013025:Glycine max; D000073871:Triggering Receptor Expressed on Myeloid Cells-1 / Q000378:metabolism
D054426:Chemokine CXCL2; C404915:Cxcl2 protein, mouse; D012503:Saponins; C517262:TREM1 protein, mouse; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D004139:Dinitrofluorobenzene
chemokine (C-X-C motif) ligand 2; contact hypersensitivity; gut microbiota; soyasaponin; triggering receptor expressed on myeloid cells-1