A Study of Noncultured Extracted Hair Follicle Outer Root Sheath Cell Suspension for Transplantation in Vitiligo.
BACKGROUND
Vitiligo surgeries have come a long way from tissue grafts to cultured and non cultured cell transplantation. Extracted hair follicle outer root sheath cell transplantation (EHF ORS) suspension is more enriched with melanocyte. In a hair bulb, there is one melanocyte for every five keratinocytes which is much higher than the epidermal melanin unit.


OBJECTIVE
To analyse the effectiveness of cultured EHF ORS and to perform objective evaluation based on clinical improvement & photographic evidence. To observe any untoward events or side effects.


METHODS
The study was open and uncontrolled. All the patients were screened at preliminary visit. Reviews were done every two weeks. The endpoint selected was six months post procedure.


METHODS
Twenty five patients of stable Vitiligo were included in the study and follicular unit were harvested by Follicular Unit Extraction method. Outer root sheath cells were extracted by trypsinization. The solution was transplanted over dermabraded recipient site. Pressure dressing was given. Patients were followed up regularly.


METHODS
Descriptive Statistics, Chi-Square.


RESULTS
Mean ± SD repigmentation was 80.15% ± 22.9% with excellent repigmentation (90-100%) in 60% of patients.


CONCLUSIONS
This method is safe, effective, and simpler than the other methods involving cell culturing and requiring a laboratory set-up but selection of patients is crucial for the success of the outcome.
Department of Dermatology, NHL Medical College, V.S. General Hospital, Ahmedabad, Gujarat, India.
Aarti N
AN
Shah
Department of Dermatology, NHL Medical College, V.S. General Hospital, Ahmedabad, Gujarat, India.
Ritu K
RK
Marfatia
Department of Dermatology, NHL Medical College, V.S. General Hospital, Ahmedabad, Gujarat, India.
Siddhartha S
SS
Saikia
Follicular Unit Extraction; melanocytes stem cells; noncultured extracted hair follicle outer root sheath suspension; vitiligo