Molecular and phenotypic characterization of Colletotrichum species associated with anthracnose disease in peppers from Sichuan Province, China.
The anthracnose caused by Colletotrichum species is an important disease that primarily causes fruit rot in pepper. Eighty-eight strains representing seven species of Colletotrichum were obtained from rotten pepper fruits in Sichuan Province, China, and characterized according to morphology and the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) sequence. Fifty-two strains were chosen for identification by phylogenetic analyses of multi-locus sequences, including the nuclear ribosomal internal transcribed spacer (ITS) region and the β-tubulin (TUB2), actin (ACT), calmodulin (CAL) and GAPDH genes. Based on the combined datasets, the 88 strains were identified as Colletotrichum gloeosporioides, C. siamense, C. fructicola, C. truncatum, C. scovillei, and C. brevisporum, and one new species was detected, described as Colletotrichum sichuanensis. Notably, C. siamense and C. scovillei were recorded for the first time as the causes of anthracnose in peppers in China. In addition, with the exception of C. truncatum, this is the first report of all of the other Colletotrichum species studied in pepper from Sichuan. The fungal species were all non-host-specific, as the isolates were able to infect not only Capsicum spp. but also Pyrus pyrifolia in pathogenicity tests. These findings suggest that the fungal species associated with anthracnose in pepper may inoculate other hosts as initial inoculum.
College of Agronomy &Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, P.R. China.
Fangling
F
Liu
College of Agronomy &Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, P.R. China.
Guiting
G
Tang
College of Agronomy &Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, P.R. China.
Xiaojuan
X
Zheng
College of Agronomy &Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, P.R. China.
Ying
Y
Li
College of Agronomy &Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, P.R. China.
Xiaofang
X
Sun
College of Agronomy &Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, P.R. China.
Xiaobo
X
Qi
College of Agronomy &Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, P.R. China.
You
Y
Zhou
College of Agronomy &Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, P.R. China.
Jing
J
Xu
College of Agronomy &Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, P.R. China.
Huabao
H
Chen
College of Agronomy &Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, P.R. China.
Xiaoli
X
Chang
College of Environmental Sciences, Sichuan Agricultural University, Chengdu, 611130, P.R. China.
Sirong
S
Zhang
College of Agronomy &Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, P.R. China.
Guoshu
G
Gong
D002212:Capsicum / Q000382:microbiology*; D002681:China; D020231:Colletotrichum / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D058885:Multilocus Sequence Typing / Q000379:methods*; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer