Polypeptide profiles of South Indian isolate of Trypanosoma evansi.
The field isolates of Trypanosoma evansi was collected from the infected cattle and it was propagated in rats. Trypanosoma evansi parasites were separated from the blood of infected rats by using diethylaminoethyl cellulose column chromatography. Whole cell lysate antigen (WCL) was prepared from purified trypanosomes by ultrasonication and centrifugation. The prepared WCL antigen was further purified by 50 % ammonium sulphate precipitation. Protein concentration of WCL antigen of T. evansi was 60 mg/ml. Protein concentration was adjusted to 1.0 mg/ml in PBS, pH 8.0 and stored at -20(0) C.   Polypeptide profiles of WCL antigen of T. evansi was determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis. A total of eight polypeptide bands of the size ranging from 25 to 85 kDa in WCL antigen of T. evansi were obtained. Five prominent bands with molecular weight of 74, 60, 53, 42 and 37 kDa and three light bands with molecular weight of 85, 34 and 25 kDa were observed.
Department of Veterinary Parasitology, C.V.Sc., S.V.V.U., YSR District, Proddatur, Andhra Pradesh India.
S
S
Sivajothi
Department of Veterinary Parasitology, C.V.Sc., S.V.V.U., YSR District, Proddatur, Andhra Pradesh India.
V C
VC
Rayulu
RARS, ANGRAU, Tirupati, Andhra Pradesh India.
B V
BV
Bhaskar Reddy
Department of Veterinary Parasitology, C.V.Sc., S.V.V.U., Tirupathi, Andhra Pradesh India.
P
P
Malakondaiah
C.V.Sc., S.V.V.U., Tirupati, Andhra Pradesh India.
D
D
Sreenivasulu
Teaching Veterinary Clinical Complex (Veterinary Medicine), C.V.Sc., S.V.V.U., YSR District, Proddatur, Andhra Pradesh India.
B
B
Sudhakara Reddy
Cattle; Polypeptide profiles; SDS-PAGE; Trypanosoma evansi; Whole cell lysate antigen