Fumaric Acid Production from Alkali-Pretreated Corncob by Fed-Batch Simultaneous Saccharification and Fermentation Combined with Separated Hydrolysis and Fermentation at High Solids Loading.
Production of fumaric acid from alkali-pretreated corncob (APC) at high solids loading was investigated using a combination of separated hydrolysis and fermentation (SHF) and fed-batch simultaneous saccharification and fermentation (SSF) by Rhizopus oryzae. Four different fermentation modes were tested to maximize fumaric acid concentration at high solids loading. The highest concentration of 41.32 g/L fumaric acid was obtained from 20 % (w/v) APC at 38 °C in the combined SHF and fed-batch SSF process, compared with 19.13 g/L fumaric acid in batch SSF alone. The results indicated that a combination of SHF and fed-batch SSF significantly improved production of fumaric acid from lignocellulose by R. oryzae than that achieved with batch SSF at high solids loading.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China. xli@njfu.edu.cn.|Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China. xli@njfu.edu.cn.|College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China. xli@njfu.edu.cn.
Xin
X
Li
College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Jin
J
Zhou
College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Shuiping
S
Ouyang
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.|Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China.|College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Jia
J
Ouyang
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.|Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037, China.|College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Qiang
Q
Yong
D000468:Alkalies / Q000737:chemistry; D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology; D050260:Carbohydrate Metabolism / Q000502:physiology; D005650:Fumarates / Q000302:isolation & purification / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007220:Industrial Waste / Q000517:prevention & control; D008031:Lignin / Q000378:metabolism*; D035261:Plant Components, Aerial / Q000737:chemistry / Q000382:microbiology; D012233:Rhizopus / Q000378:metabolism*; D012972:Sodium Hydroxide / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry* / Q000382:microbiology*
D000468:Alkalies; D005650:Fumarates; D007220:Industrial Waste; C036909:lignocellulose; D012972:Sodium Hydroxide; C032005:fumaric acid; D008031:Lignin; D005947:Glucose
Alkali-pretreated corncob; Fed-batch simultaneous saccharification and fermentation; Fumaric acid; High solids loading; Rhizopus oryzae