Linear and Non-Linear Optical Imaging of Cancer Cells with Silicon Nanoparticles.
New approaches for visualisation of silicon nanoparticles (SiNPs) in cancer cells are realised by means of the linear and nonlinear optics in vitro. Aqueous colloidal solutions of SiNPs with sizes of about 10-40 nm obtained by ultrasound grinding of silicon nanowires were introduced into breast cancer cells (MCF-7 cell line). Further, the time-varying nanoparticles enclosed in cell structures were visualised by high-resolution structured illumination microscopy (HR-SIM) and micro-Raman spectroscopy. Additionally, the nonlinear optical methods of two-photon excited fluorescence (TPEF) and coherent anti-Stokes Raman scattering (CARS) with infrared laser excitation were applied to study the localisation of SiNPs in cells. Advantages of the nonlinear methods, such as rapid imaging, which prevents cells from overheating and larger penetration depth compared to the single-photon excited HR-SIM, are discussed. The obtained results reveal new perspectives of the multimodal visualisation and precise detection of the uptake of biodegradable non-toxic SiNPs by cancer cells and they are discussed in view of future applications for the optical diagnostics of cancer tumours.
Leibniz Institute of Photonic Technology, Jena 07745, Germany. elen.tolstik@ipht-jena.de.
Elen
E
Tolstik
Physics Department, Lomonosov Moscow State University, Moscow 119991, Russia. osminkina@mig.phys.msu.ru.|Interational Laboratory "Bio-Nanophotonics", National Research Nuclear University "Moscow Engineering Physics Institute", Moscow 115409, Russia. osminkina@mig.phys.msu.ru.
Liubov A
LA
Osminkina
Leibniz Institute of Photonic Technology, Jena 07745, Germany. denis.akimov@ipht-jena.de.
Denis
D
Akimov
Physics Department, Lomonosov Moscow State University, Moscow 119991, Russia. mgongalsky@gmail.com.
Maksim B
MB
Gongalsky
Institute of Theoretical and Experimental Biophysics, Russian Academy of Science, Pushino 142290, Russia. centavr42@mail.ru.
Andrew A
AA
Kudryavtsev
Physics Department, Lomonosov Moscow State University, Moscow 119991, Russia. timoshen@physics.msu.ru.|Interational Laboratory "Bio-Nanophotonics", National Research Nuclear University "Moscow Engineering Physics Institute", Moscow 115409, Russia. timoshen@physics.msu.ru.
Victor Yu
VY
Timoshenko
Leibniz Institute of Photonic Technology, Jena 07745, Germany. heintzmann@gmail.com.|Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller-University, Jena 07743, Germany. heintzmann@gmail.com.
Rainer
R
Heintzmann
Leibniz Institute of Photonic Technology, Jena 07745, Germany. vladimir.sivakov@ipht-jena.de.
Vladimir
V
Sivakov
Leibniz Institute of Photonic Technology, Jena 07745, Germany. juergen.popp@ipht-jena.de.|Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller-University, Jena 07743, Germany. juergen.popp@ipht-jena.de.
Jürgen
J
Popp
D006801:Humans; D061986:MCF-7 Cells; D008853:Microscopy; D064847:Multimodal Imaging / Q000379:methods*; D053770:Nanowires / Q000648:ultrastructure*; D009369:Neoplasms / Q000175:diagnosis*; D061848:Optical Imaging / Q000379:methods; D010316:Particle Size; D012825:Silicon / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods
D012825:Silicon
Raman spectroscopy; coherent anti-Stokes Raman scattering (CARS); high-resolution structured illumination microscopy (HR-SIM); multimodal bioimaging; nanowires; silicon nanoparticles; two-photon excited fluorescence (TPEF)