EIGER detector: application in macromolecular crystallography.
The development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. The new EIGER detector features a pixel size of 75 × 75 µm, frame rates of up to 3000 Hz and a dead time as low as 3.8 µs. An EIGER 1M and EIGER 16M were tested on Swiss Light Source beamlines X10SA and X06SA for their application in macromolecular crystallography. The combination of fast frame rates and a very short dead time allows high-quality data acquisition in a shorter time. The ultrafine ϕ-slicing data-collection method is introduced and validated and its application in finding the optimal rotation angle, a suitable rotation speed and a sufficient X-ray dose are presented. An improvement of the data quality up to slicing at one tenth of the mosaicity has been observed, which is much finer than expected based on previous findings. The influence of key data-collection parameters on data quality is discussed.
Swiss Light Source, Paul Scherrer Institute, 5232 Villigen, Switzerland.
Arnau
A
Casanas
Swiss Light Source, Paul Scherrer Institute, 5232 Villigen, Switzerland.
Rangana
R
Warshamanage
Swiss Light Source, Paul Scherrer Institute, 5232 Villigen, Switzerland.
Aaron D
AD
Finke
Swiss Light Source, Paul Scherrer Institute, 5232 Villigen, Switzerland.
Ezequiel
E
Panepucci
Swiss Light Source, Paul Scherrer Institute, 5232 Villigen, Switzerland.
Vincent
V
Olieric
Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main, Germany.
Anne
A
Nöll
Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main, Germany.
Robert
R
Tampé
DECTRIS Ltd, Taefernweg 1, 5405 Baden-Dättwil, Switzerland.
Stefan
S
Brandstetter
DECTRIS Ltd, Taefernweg 1, 5405 Baden-Dättwil, Switzerland.
Andreas
A
Förster
DECTRIS Ltd, Taefernweg 1, 5405 Baden-Dättwil, Switzerland.
Marcus
M
Mueller
DECTRIS Ltd, Taefernweg 1, 5405 Baden-Dättwil, Switzerland.
Clemens
C
Schulze-Briese
Swiss Light Source, Paul Scherrer Institute, 5232 Villigen, Switzerland.
Oliver
O
Bunk
Swiss Light Source, Paul Scherrer Institute, 5232 Villigen, Switzerland.
Meitian
M
Wang
D000818:Animals; D002645:Chickens; D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D007328:Insulin / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry; D017785:Photons; D011506:Proteins / Q000737:chemistry*; D013552:Swine
D007328:Insulin; D011506:Proteins; D009113:Muramidase
EIGER detector; X-ray detectors; data-collection strategy; macromolecular crystallography