Multifunctional nanocrystalline calcium phosphates loaded with Tetracycline antibiotic combined with human adipose derived mesenchymal stromal stem cells (hASCs).
Osteoconductive drug delivery system composed of nanocrystalline calcium phosphates (Ca10(PO4)6(OH)2/β-Ca3(PO4)2) co-doped with Yb(3+)/Er(3+) ions loaded with Tetracycline antibiotic (TC) was developed. Their effect on human adipose derived mesenchymal stromal stem cells (hASCs) as a potential reconstructive biomaterial for bone tissue regeneration was studied. The XRD and TEM measurements were used in order to determine the crystal structure and morphology of the final products. The characteristics of nanocomposites with the TC and hASCs as potential regenerative materials as well as the antimicrobial activity of the nanoparticles against: Staphylococcus aureus ATCC 25923 as a model of the Gram-positive bacteria, Escherichia coli ATCC 8739 of the Gram-negative bacteria, were shown. These combinations can be a promising material for theranostic due to its regenerative, antimicrobial and fluorescent properties.
Wroclaw University of Environmental and Life Sciences, Faculty of Biology, Kozuchowska 5b, 50-631 Wroclaw, Poland; Wroclaw Research Centre EIT+, Stablowicka 147, 54-066 Wroclaw, Poland.
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Marycz
Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland.
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Pazik
Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland.
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Zawisza
Wroclaw Medical University, Faculty of Pharmacy, Borowska 211 A, 50-566 Wroclaw, Poland.
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K
Wiglusz
Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Norwida 31, 50-375 Wroclaw, Poland.
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Maredziak
Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland.
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Sobierajska
Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland. Electronic address: R.Wiglusz@int.pan.wroc.pl.
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Wiglusz
D000273:Adipose Tissue / Q000166:cytology; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D015703:Antigens, CD / Q000378:metabolism; D001861:Bone Regeneration / Q000187:drug effects; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004926:Escherichia coli / Q000187:drug effects; D005063:Europium / Q000737:chemistry; D006801:Humans; D007477:Ions / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D053761:Nanocomposites / Q000737:chemistry*; D010316:Particle Size; D013211:Staphylococcus aureus / Q000187:drug effects; D013754:Tetracyclines / Q000737:chemistry* / Q000494:pharmacology; D015018:Ytterbium / Q000737:chemistry
D000900:Anti-Bacterial Agents; D015703:Antigens, CD; D002130:Calcium Phosphates; D007477:Ions; D013754:Tetracyclines; D005063:Europium; D015018:Ytterbium
Apatites - calcium phosphates; Mesenchymal stem cells; Nanotechnology; Regenerative medicine; Up-conversion