Hook2, a microtubule-binding protein, interacts with Par6α and controls centrosome orientation during polarized cell migration.
Polarity protein complexes function during polarized cell migration and a subset of these proteins localizes to the reoriented centrosome during this process. Despite these observations, the mechanisms behind the recruitment of these polarity complexes such as the aPKC/PAR6α complex to the centrosome are not well understood. Here we identify Hook2 as an interactor for the aPKC/PAR6α complex that functions to localize this complex at the centrosome. We first demonstrate that Hook2 is essential for the polarized Golgi re-orientation towards the migration front. Depletion of Hook2 results in a decrease of PAR6α at the centrosome during cell migration, while overexpression of Hook2 in cells induced the formation of aggresomes with the recruitment of PAR6α, aPKC and PAR3. In addition, we demonstrate that the interaction between the C-terminal domain of Hook2 and the aPKC-binding domain of PAR6α localizes PAR6α to the centrosome during cell migration. Our data suggests that Hook2, a microtubule binding protein, plays an important role in the regulation of PAR6α recruitment to the centrosome to bridge microtubules and the aPKC/PAR complex. This data reveals how some of the polarity protein complexes are recruited to the centrosome and might regulate pericentriolar and microtubule organization and potentially impact on polarized migration.
Aix-Marseille Univ, CNRS, UMR 7288, Developmental Biology Institute of Marseille (IBDM), case 907, 13288 Marseille, cedex 09, France.
Emilie
E
Pallesi-Pocachard
Aix-Marseille Univ, CNRS, UMR 7288, Developmental Biology Institute of Marseille (IBDM), case 907, 13288 Marseille, cedex 09, France.
Elsa
E
Bazellieres
Aix-Marseille Univ, CNRS, UMR 7288, Developmental Biology Institute of Marseille (IBDM), case 907, 13288 Marseille, cedex 09, France.
Annelise
A
Viallat-Lieutaud
Aix-Marseille Univ, CNRS, UMR 7288, Developmental Biology Institute of Marseille (IBDM), case 907, 13288 Marseille, cedex 09, France.
Marie-Hélène
MH
Delgrossi
Aix-Marseille Univ, CNRS, UMR 7288, Developmental Biology Institute of Marseille (IBDM), case 907, 13288 Marseille, cedex 09, France.
Magali
M
Barthelemy-Requin
Aix-Marseille Univ, CNRS, UMR 7288, Developmental Biology Institute of Marseille (IBDM), case 907, 13288 Marseille, cedex 09, France.
André
A
Le Bivic
Aix-Marseille Univ, CNRS, UMR 7288, Developmental Biology Institute of Marseille (IBDM), case 907, 13288 Marseille, cedex 09, France.
Dominique
D
Massey-Harroche
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics*; D016764:Cell Polarity / Q000235:genetics; D018385:Centrosome / Q000378:metabolism; D020090:Chromosome Segregation / Q000235:genetics; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism; D006801:Humans; D002479:Inclusion Bodies / Q000235:genetics; D061986:MCF-7 Cells; D008565:Membrane Proteins / Q000235:genetics*; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008870:Microtubules / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011493:Protein Kinase C / Q000235:genetics*
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C521349:Hook2 protein, human; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; C471157:PARD3B protein, human; C499657:PARD6A protein, human; C115015:PKC-3 protein; D011493:Protein Kinase C