Monitoring the evolution of boron doped porous diamond electrode on flexible retinal implant by OCT and in vivo impedance spectroscopy.
Nanocrystalline Boron doped Diamond proved to be a very attractive material for neural interfacing, especially with the retina, where reduce glia growth is observed with respect to other materials, thus facilitating neuro-stimulation over long terms. In the present study, we integrated diamond microelectrodes on a polyimide substrate and investigated their performances for the development of neural prosthesis. A full description of the microfabrication of the implants is provided and their functionalities are assessed using cyclic voltammetry and electrochemical impedance spectroscopy. A porous structure of the electrode surface was thus revealed and showed promising properties for neural recording or stimulation. Using the flexible implant, we showed that is possible to follow in vivo the evolution of the electric contact between the diamond electrodes and the retina over 4months by using electrochemical impedance spectroscopy. The position of the implant was also monitored by optical coherence tomography to corroborate the information given by the impedance measurements. The results suggest that diamond microelectrodes are very good candidates for retinal prosthesis.
CEA-LIST, Diamond Sensors Laboratory, Gif-sur-Yvette 91191, France. Electronic address: clement.hebert@icn2.cat.
Clément
C
Hébert
Université Paris-Est, ESYCOM-ESIEE Paris, Noisy le Grand, France.
Myline
M
Cottance
INSERM, U968, Institut de la Vision, Paris, France.
Julie
J
Degardin
CEA-LIST, Diamond Sensors Laboratory, Gif-sur-Yvette 91191, France.
Emmanuel
E
Scorsone
Université Paris-Est, ESYCOM-ESIEE Paris, Noisy le Grand, France.
Lionel
L
Rousseau
Université Paris-Est, ESYCOM-ESIEE Paris, Noisy le Grand, France.
Gaelle
G
Lissorgues
CEA-LIST, Diamond Sensors Laboratory, Gif-sur-Yvette 91191, France.
Philippe
P
Bergonzo
INSERM, U968, Institut de la Vision, Paris, France.
Serge
S
Picaud
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001895:Boron / Q000737:chemistry*; D018130:Diamond / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D004195:Disease Models, Animal; D055664:Electrochemical Techniques; D004567:Electrodes, Implanted; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D051381:Rats; D012174:Retinitis Pigmentosa / Q000378:metabolism / Q000473:pathology; D041623:Tomography, Optical Coherence; D057488:Visual Prosthesis
D001672:Biocompatible Materials; D018130:Diamond; D001895:Boron