DIANA-TarBase and DIANA Suite Tools: Studying Experimentally Supported microRNA Targets.
microRNAs (miRNAs) are short non-coding RNAs (∼22 nts) present in animals, plants, and viruses. They are considered central post-transcriptional regulators of gene expression and are key components in a great number of physiological and pathological conditions. The accurate characterization of their targets is considered essential to a series of applications and basic or applied research settings. DIANA-TarBase (http://www.microrna.gr/tarbase) was initially launched in 2006. It is a reference repository indexing experimentally derived miRNA-gene interactions in different cell types, tissues, and conditions across numerous species. This unit focuses on the study of experimentally supported miRNA-gene interactions, as well as their functional interpretation through the use of available tools in the DIANA suite (http://www.microrna.gr). The proposed use-case scenarios are presented in protocols, describing how to utilize the DIANA-TarBase database and DIANA-microT-CDS server and perform miRNA-targeted pathway analysis with DIANA-miRPath-v3. All analyses are directly invoked or initiated from DIANA-TarBase. © 2016 by John Wiley & Sons, Inc.
DIANA-Lab, Department of Electrical & Computer Engineering, University of Thessaly, Volos, Greece.|Hellenic Pasteur Institute, Athens, Greece.
Maria D
MD
Paraskevopoulou
DIANA-Lab, Department of Electrical & Computer Engineering, University of Thessaly, Volos, Greece.|Hellenic Pasteur Institute, Athens, Greece.
Ioannis S
IS
Vlachos
DIANA-Lab, Department of Electrical & Computer Engineering, University of Thessaly, Volos, Greece.|Hellenic Pasteur Institute, Athens, Greece.
Artemis G
AG
Hatzigeorgiou
D000818:Animals; D019295:Computational Biology / Q000295:instrumentation / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012984:Software
D035683:MicroRNAs
RNAi; experimental methodology; high-throughput; in silico predictions; interaction; miRNA interactome; microRNA; pathway; target