The RNA Editing Factor WSP1 Is Essential for Chloroplast Development in Rice.
Although the multiple organellar RNA editing factors (MORFs) in the plastids of Arabidopsis thaliana have been extensively studied, molecular details underlying how MORFs affect plant development in other species, particularly in rice, remain largely unknown. Here we describe the characterization of wsp1, a rice mutant with white-stripe leaves and panicles. Notably, wsp1 exhibited nearly white immature panicles at the heading stage. Transmission electron microscopy analysis and chlorophyll content measurement revealed a chloroplast developmental defect and reduced chlorophyll accumulation in wsp1. Positional cloning of WSP1 found a point mutation in Os04g51280, whose putative product shares high sequence similarity with MORF proteins. Complementation experiments demonstrated that WSP1 was responsible for the variegated phenotypes of wsp1. WSP1 is localized to chloroplasts and the point mutation in wsp1 affected the editing of multiple organellar RNA sites. Owing to the defect in plastid RNA editing, chloroplast ribosome biogenesis and ndhA splicing were also impaired in wsp1, which may affect normal chloroplast development in the leaves and panicles at the heading stage. Together, our results demonstrate the importance of rice WSP1 protein in chloroplast development and broaden our knowledge about MORF family members in rice.
Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement, The Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China.
Zhiguo
Z
Zhang
Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement, The Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China.
Xuean
X
Cui
Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement, The Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China.
Yanwei
Y
Wang
Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement, The Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China.
Jinxia
J
Wu
Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement, The Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China. Electronic address: guxiaofeng@caas.cn.
Xiaofeng
X
Gu
Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement, The Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China. Electronic address: lutiegang@caas.cn.
Tiegang
T
Lu
D002467:Cell Nucleus / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D007438:Introns; D009154:Mutation; D012275:Oryza / Q000235:genetics / Q000254:growth & development*; D010788:Photosynthesis / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D017393:RNA Editing; D018749:RNA, Plant / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*
D010940:Plant Proteins; D018749:RNA, Plant; D016601:RNA-Binding Proteins
PEP complex and ribosome biosynthesis; RNA editing; chloroplast development; rice