Epidemiological characterization of Acinetobacter baumannii bloodstream isolates from a Chinese Burn Institute: A three-year study.
Acinetobacter baumannii infection is a serious threat to burn patients. Bacteremia due to A. baumannii is becoming the most common cause of mortality following burn. However, the epidemiology of A. baumannii causing burn-related bloodstream infections has rarely been reported. We retrospectively collected 81 A. baumannii isolates from the bloodstream of burn patients over a three-year period. Antibiotic susceptibility tests, the prevalence of antibiotic-resistant genes and sequence typing (ST) were conducted to characterize these strains. Most of the isolates showed an extensive drug-resistant phenotype. The resistance frequencies to imipenem and meropenem were 94% and 91%, respectively. The blaOXA-23-like gene, AmpC, IS-AmpC, PER and SIM are the five most prevalent resistant genes, and their prevalence rates are 93% (75/81), 86% (70/81), 73% (59/81), 73% (59/81) and 52% (42/81), respectively. The 81 isolates were grouped into 10 known and 18 unknown ST types, with ST368 (38%) being the most prevalent. Except for ST457 and four new types (STn2, STn6, STn11 and STn14), the remaining 23 ST types belonged to one clonal complex 92, which is most common among clinical isolate in China. The above results indicated that ST368 isolates possessing both the blaOXA-23-like gene and ampC gene were the main culprits of the increasing nosocomial A. baumannii infection in this study. More attention should be paid to monitoring the molecular epidemiology of A. baumannii isolates from burn patients to prevent further distribution. Such information may help clinicians with therapeutic decisions and infection control in the Burns Institute.
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
Guangtao
G
Huang
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
Supeng
S
Yin
Medical Examination Center, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
Lijuan
L
Xiang
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
Yali
Y
Gong
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
Kedai
K
Sun
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
Xiaoqiang
X
Luo
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
Cheng
C
Zhang
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
Zichen
Z
Yang
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
Liuyang
L
Deng
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
Bei
B
Jiang
Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL, United States.
Shouguang
S
Jin
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
Jing
J
Chen
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China. Electronic address: yizhipen@sina.com.
Yizhi
Y
Peng
D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016470:Bacteremia / Q000453:epidemiology / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002056:Burns / Q000453:epidemiology / Q000382:microbiology*; D002681:China / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012189:Retrospective Studies; D001618:beta-Lactamases / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; C577373:beta-lactamase OXA-51, Acinetobacter baumannii
Acinetobacter baumannii; Bacteremia; Extended drug-resistant; Multilocus sequence typing (MLST); β-lactam