Orally Administrated Whole Yeast Vaccine Against Porcine Epidemic Diarrhea Virus Induced High Levels of IgA Response in Mice and Piglets.
The mucosal immune response against the porcine epidemic diarrhea virus (PEDV) is very important in piglets. To develop a PEDV vaccine suitable for inducing high levels of intestinal IgA in piglets, recombinant yeast expressing the PEDV S1 gene was constructed and tested by oral immunization of mice and piglets. The S1-specific IgG and IgA were tested at 0, 14, and 28 days postimmunization (dpi) in mice. Compared to the control group, the mice treated with S1 expressing yeast, demonstrated significantly higher levels of IgG and IgA against PEDV from 14 dpi onward. The recombinant yeast inducing a fecal IgA response in piglets was also tested. PEDV-specific IgA could be detected at 7 dpi and increased to 28 dpi. We demonstrated that whole recombinant yeast can be used as a PEDV vaccine vector for inducing high levels of IgA against PEDV in piglets. This could be a good vaccine candidate for PEDV control in piglets.
College of Veterinary Medicine, Northwest A&F University , Shaanxi Yangling, China .
Xinglong
X
Wang
College of Veterinary Medicine, Northwest A&F University , Shaanxi Yangling, China .
Zhenbing
Z
Wang
College of Veterinary Medicine, Northwest A&F University , Shaanxi Yangling, China .
Hongyu
H
Xu
College of Veterinary Medicine, Northwest A&F University , Shaanxi Yangling, China .
Biao
B
Xiang
College of Veterinary Medicine, Northwest A&F University , Shaanxi Yangling, China .
Ruiyi
R
Dang
College of Veterinary Medicine, Northwest A&F University , Shaanxi Yangling, China .
Zengqi
Z
Yang
D000284:Administration, Oral; D000818:Animals; D018352:Coronavirus Infections / Q000517:prevention & control*; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D053485:Porcine epidemic diarrhea virus / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012440:Saccharomyces / Q000235:genetics / Q000276:immunology; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014170:Transformation, Genetic; D014765:Viral Vaccines / Q000008:administration & dosage / Q000627:therapeutic use*
D007070:Immunoglobulin A; D011994:Recombinant Proteins; D064370:Spike Glycoprotein, Coronavirus; D014765:Viral Vaccines
S glycoprotein; mucosal immune response; oral administration; transmissible gastroenteritis virus; yeast expression