Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds.
Effects of strontium and lithium ion doping on the biological properties of bioactive glass (BAG) porous scaffolds have been checked in vitro and in vivo. BAG scaffolds were prepared by conventional glass melting route and subsequently, scaffolds were produced by evaporation of fugitive pore formers. After thorough physico-chemical and in vitro cell characterization, scaffolds were used for pre-clinical study. Soft and hard tissue formation in a rabbit femoral defect model after 2 and 4 months, were assessed using different tools. Histological observations showed excellent osseous tissue formation in Sr and Li + Sr scaffolds and moderate bone regeneration in Li scaffolds. Fluorochrome labeling studies showed wide regions of new bone formation in Sr and Li + Sr doped samples as compared to Li doped samples. SEM revealed abundant collagenous network and minimal or no interfacial gap between bone and implant in Sr and Li + Sr doped samples compared to Li doped samples. Micro CT of Li + Sr samples showed highest degree of peripheral cancellous tissue formation on periphery and cortical tissues inside implanted samples and vascularity among four compositions. Our findings suggest that addition of Sr and/or Li alters physico-chemical properties of BAG and promotes early stage in vivo osseointegration and bone remodeling that may offer new insight in bone tissue engineering.
Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, India.
Pintu Kumar
PK
Khan
Bioceramics and Coating Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata, India.
Arnab
A
Mahato
Bioceramics and Coating Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata, India.
Biswanath
B
Kundu
Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, India.
Samit K
SK
Nandi
Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, India.
Prasenjit
P
Mukherjee
Bioceramics and Coating Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata, India.
Someswar
S
Datta
Non-Oxide Ceramics and Composites Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata, India.
Soumya
S
Sarkar
Institute of Animal Health and Veterinary Biologicals, Animal Resources Development Department, Kolkata, India.
Jayanta
J
Mukherjee
Cancer Biology and Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
Shalini
S
Nath
Bioceramics and Coating Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata, India.
Vamsi K
VK
Balla
Cancer Biology and Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
Chitra
C
Mandal
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration; D005264:Femoral Fractures / Q000628:therapy; D005456:Fluorescent Dyes; D017102:Fracture Healing; D005898:Glass / Q000737:chemistry*; D008094:Lithium / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D016062:Porosity; D011817:Rabbits; D013324:Strontium / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography
D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D008094:Lithium; D013324:Strontium