Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1.
Mannheimia haemolytica is a Gram negative bacterium that is part of the bovine respiratory disease, which causes important economic losses in the livestock industry. In the present work, the interaction between M. haemolytica A1 and bovine lactoferrin (BLf) was studied. This iron-chelating glycoprotein is part of the mammalian innate-immune system and is present in milk and mucosal secretions; Lf is also contained in neutrophils secondary granules, which release this glycoprotein at infection sites. It was evidenced that M. haemolytica was not able to use iron-charged BLf (BholoLf) as a sole iron source; nevertheless, iron-lacked BLf (BapoLf) showed a bactericidal effect against M. haemolytica with MIC of 4.88 ± 1.88 and 7.31 ± 1.62 μM for M. haemolytica strain F (field isolate) and M. haemolytica strain R (reference strain), respectively. Through overlay assays and 2-D electrophoresis, two OMP of 32.9 and 34.2 kDa with estimated IP of 8.18 and 9.35, respectively, were observed to bind both BapoLf and BholoLf; these OMP were identified by Maldi-Tof as OmpA (heat-modifiable OMP) and a membrane protein (porin). These M. haemolytica BLf binding proteins could be interacting in vivo with both forms of BLf depending on the iron state of the bovine.
Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Avenida Instituto Politécnico Nacional No. 2508, Colonia San Pedro Zacatenco, CP 07360, Ciudad de México, Mexico.
Luisa
L
Samaniego-Barrón
Facultad de Medicina Veterinaria y Zootecnia Dr. Norberto Treviño Zapata, Universidad Autónoma de Tamaulipas, Carretera a Cd. Mante Km 5, CP 87000, Ciudad Victoria, Tamaulipas, Mexico.
Sarahí
S
Luna-Castro
Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Avenida Instituto Politécnico Nacional No. 2508, Colonia San Pedro Zacatenco, CP 07360, Ciudad de México, Mexico.
Carolina
C
Piña-Vázquez
Departamento de Microbiología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México (UNAM), Av. Universidad 3000, Cd. Universitaria, Coyoacán, CP 04510, Ciudad de México, Mexico.
Francisco
F
Suárez-Güemes
Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Avenida Instituto Politécnico Nacional No. 2508, Colonia San Pedro Zacatenco, CP 07360, Ciudad de México, Mexico. mireya@cell.cinvestav.mx.
Mireya
0000-0002-6188-4220
M
de la Garza
D000818:Animals; D001059:Apoproteins / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D007113:Immunity, Innate; D007781:Lactoferrin / Q000276:immunology / Q000378:metabolism*; D016978:Mannheimia haemolytica / Q000276:immunology / Q000378:metabolism*; D062105:Molecular Docking Simulation; D012766:Pasteurellosis, Pneumonic / Q000276:immunology / Q000378:metabolism
D001059:Apoproteins; D001425:Bacterial Outer Membrane Proteins; C039223:apolactoferrin; D007781:Lactoferrin