Low Dosage of Chitosan Supplementation Improves Intestinal Permeability and Impairs Barrier Function in Mice.
The purpose of this study was to explore relationships between low dose dietary supplementation with chitosan (COS) and body weight, feed intake, intestinal barrier function, and permeability in mice. Twenty mice were randomly assigned to receive an unadulterated control diet (control group) or a dietary supplementation with 30 mg/kg dose of chitosan (COS group) for two weeks. Whilst no significant differences were found between the conditions for body weight or food and water intake, mice in the COS group had an increased serum D-lactate content (P < 0.05) and a decreased jejunal diamine oxidase (DAO) activity (P < 0.05). Furthermore, mice in COS group displayed a reduced expression of occludin and ZO-1 (P < 0.05) and a reduced expression of occludin in the ileum (P < 0.05). The conclusion drawn from these findings showed that although 30 mg/kg COS-supplemented diet had no effect on body weight or feed intake in mice, this dosage may compromise intestinal barrier function and permeability. This research will contribute to the guidance on COS supplements.
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China.
Guiping
0000-0002-6850-9626
G
Guan
Hunan Institute of Animal and Veterinary Science, Changsha, Changsha 410131, China.
Hongbing
0000-0002-7362-7854
H
Wang
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China.
Hanhui
H
Peng
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China.
Guanya
G
Li
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D048271:Chitosan / Q000008:administration & dosage* / Q000494:pharmacology*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005247:Feeding Behavior / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D007422:Intestines / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology*; D008813:Mice, Inbred ICR; D010539:Permeability / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D062725:Tight Junction Proteins / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D062725:Tight Junction Proteins; D048271:Chitosan