Characterization of Lipid-Based Hexosomes as Versatile Vaccine Carriers.
Subunit vaccines typically show insufficient immunogenicity. To address this issue, we developed a novel self-adjuvanting particulate carrier system based upon the lipids phytantriol (Phy) and mannide monooleate (MaMo). Phy is a lipid known to form nonlamellar phases in fully hydrated systems, whereas MaMo has been found to promote immune responses in emulsion form. A bulk phase composition of Phy/MaMo (14 wt %) showed hexagonal (HII) phase behavior over a practical temperature range (including room and body temperature), and was therefore used for particle development. Hexosomes stabilized with different concentrations of either poloxamer 407, Myrj 59, or Pluronic F108 were successfully prepared. To demonstrate the versatile nature of these systems, the particles were further modified with either positively or negatively charged lipids and loaded with model antigens, while maintaining the HII structure. These hexosomes are structurally robust and amenable to customization, rendering them suitable as antigen delivery carriers.
Department of Pharmacy, Pharmaceutical Technology and Biopharmacy, Ludwig-Maximilians-Universität München , Butenandtstraße 5-13, DE-81377 Munich, Germany.
Letícia
L
Rodrigues
Physics Department, Soft Matter Physics Group, Technische Universität München , James-Franck-Straße 1, DE-85748 Garching, Germany.
Konstantinos
K
Kyriakos
Physics Department and Institute for Advanced Study, Walter Schottky Institute, Technische Universität München , Am Coulombwall 4a, DE-85748 Garching, Germany.
Fabian
F
Schneider
Physics Department and Institute for Advanced Study, Walter Schottky Institute, Technische Universität München , Am Coulombwall 4a, DE-85748 Garching, Germany.
Hendrik
H
Dietz
Department of Pharmacy, Pharmaceutical Technology and Biopharmacy, Ludwig-Maximilians-Universität München , Butenandtstraße 5-13, DE-81377 Munich, Germany.
Gerhard
G
Winter
Physics Department, Soft Matter Physics Group, Technische Universität München , James-Franck-Straße 1, DE-85748 Garching, Germany.
Christine M
CM
Papadakis
Department of Pharmacy, Pharmaceutical Technology and Biopharmacy, Ludwig-Maximilians-Universität München , Butenandtstraße 5-13, DE-81377 Munich, Germany.
Madlen
M
Hubert
D020285:Cryoelectron Microscopy; D004337:Drug Carriers / Q000737:chemistry*; D000067493:Dynamic Light Scattering; D005233:Fatty Alcohols / Q000737:chemistry; D050866:Liquid Crystals / Q000737:chemistry / Q000648:ultrastructure; D008353:Mannitol / Q000031:analogs & derivatives / Q000737:chemistry; D009829:Oleic Acids / Q000737:chemistry; D010316:Particle Size; D014612:Vaccines / Q000737:chemistry*
D004337:Drug Carriers; D005233:Fatty Alcohols; D009829:Oleic Acids; D014612:Vaccines; C001681:mannide monooleate; D008353:Mannitol; C508873:3,7,11,15-tetramethyl-1,2,3-hexadecanetriol
hexosomes; lyotropic liquid crystals; mannide monooleate; phytantriol; vaccine delivery