Ceftriaxone-Resistant Neisseria gonorrhoeae Isolates (2010 to 2014) in France Characterized by Using Whole-Genome Sequencing.
Two extended-spectrum cephalosporin-resistant Neisseria gonorrhoeae isolates were discovered among 6,340 (0.03%) French isolates between 2010 and 2014. One isolate corresponded to the F89 multidrug-resistant N. gonorrhoeae isolate harboring a penA mosaic; whole-genome sequencing highlighted an additional R251H substitution in the ftsX gene recently involved in cephalosporin resistance. The other, ceftriaxone-resistant isolate (MIC, 0.25 mg/liter) harbored the PBP2 pattern XXXVI plus a P551S substitution and belonged to sequence type ST1579 (multilocus sequence typing [MLST]).
Laboratory of Bacteriology, University Hospital of Dijon, Dijon, France.|APHP, Lariboisière-St Louis-Fernand Widal Hospital, Laboratory of Bacteriology, Associated Laboratory for the National Reference Centre for Gonococci, Paris, France.
Claire
C
de Curraize
APHP, Lariboisière-St Louis-Fernand Widal Hospital, Laboratory of Bacteriology, Associated Laboratory for the National Reference Centre for Gonococci, Paris, France.
Sylvain
S
Kumanski
APHP, Lariboisière-St Louis-Fernand Widal Hospital, Laboratory of Bacteriology, Associated Laboratory for the National Reference Centre for Gonococci, Paris, France.|University Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, Paris, France.|INSERM, IAME, UMR 1137, Paris, France.
Maïté
M
Micaëlo
French Institute for Public Heath Surveillance (InVS), Department of Infectious Diseases, Saint-Maurice, France.
Nelly
N
Fournet
French Institute for Public Heath Surveillance (InVS), Department of Infectious Diseases, Saint-Maurice, France.
Guy
G
La Ruche
APHP, Lariboisière-St Louis-Fernand Widal Hospital, Laboratory of Bacteriology, Associated Laboratory for the National Reference Centre for Gonococci, Paris, France.
Fabienne
F
Meunier
APHP, Lariboisière-St Louis-Fernand Widal Hospital, Laboratory of Bacteriology, Associated Laboratory for the National Reference Centre for Gonococci, Paris, France.|University Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, Paris, France.|INSERM, IAME, UMR 1137, Paris, France.
Rishma
R
Amarsy
APHP, Lariboisière-St Louis-Fernand Widal Hospital, Laboratory of Bacteriology, Associated Laboratory for the National Reference Centre for Gonococci, Paris, France.|University Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, Paris, France.|INSERM, IAME, UMR 1137, Paris, France.
Hervé
H
Jacquier
APHP, Lariboisière-St Louis-Fernand Widal Hospital, Laboratory of Bacteriology, Associated Laboratory for the National Reference Centre for Gonococci, Paris, France.|University Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, Paris, France.|INSERM, IAME, UMR 1137, Paris, France.
Emmanuelle
E
Cambau
Alfred Fournier Institute, National Reference Centre for Gonococci, Paris, France.
Agathe
A
Goubard
APHP, Lariboisière-St Louis-Fernand Widal Hospital, Laboratory of Bacteriology, Associated Laboratory for the National Reference Centre for Gonococci, Paris, France beatrice.bercot@aphp.fr.|University Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, Paris, France.|INSERM, IAME, UMR 1137, Paris, France.
Béatrice
0000-0003-1603-3635
B
Bercot
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D020682:Cefixime / Q000494:pharmacology; D002443:Ceftriaxone / Q000494:pharmacology*; D018797:Cell Cycle Proteins / Q000235:genetics; D018550:Cephalosporin Resistance / Q000235:genetics*; D005602:France; D016680:Genome, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; C102286:FtsX protein, bacteria; D002443:Ceftriaxone; D020682:Cefixime