Characteristics and complete genome analysis of a novel jumbo phage infecting pathogenic Bacillus pumilus causing ginger rhizome rot disease.
Tailed phages with genomes larger than 200 kbp are classified as jumbo phage and exhibit extremely high diversity. In this study, a novel jumbo phage, vB_BpuM_BpSp, infecting pathogenic Bacillus pumilus, the cause of ginger rhizome rot disease, was isolated. Notable features of phage vB_BpuM_BpSp are the large phage capsid of 137 nm and baseplate-attached curly tail fibers. The genome of the phage is 255,569 bp in size with G+C content of 25.9 %, and it shows low similarity to known biological entities. The phage genome contains 318 predicted coding sequences. Among these predicted coding sequences, 26 genes responsible for nucleotide metabolism were found, and seven structural genes could be identified. The findings of this study provide new understanding of the genetic diversity of phages.
Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, People's Republic of China.
Yihui
Y
Yuan
Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, People's Republic of China. mygao@wh.iov.cn.
Meiying
M
Gao
D017103:Bacillus Phages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D000069979:Bacillus pumilus / Q000302:isolation & purification / Q000821:virology*; D001482:Base Composition; D002213:Capsid / Q000648:ultrastructure; D004279:DNA, Viral / Q000235:genetics*; D023061:Gene Order; D016679:Genome, Viral; D020939:Zingiber officinale / Q000382:microbiology; D046529:Microscopy, Electron, Transmission; D016366:Open Reading Frames; D010935:Plant Diseases / Q000382:microbiology; D027343:Rhizome / Q000382:microbiology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D004279:DNA, Viral