Sensitivity to Enterocins of Thermophilic Campylobacter spp. from Different Poultry Species.
Campylobacter spp. have been isolated from various animals, including poultry. They are rapidly transmitted throughout broiler sheds by the fecal-oral route. A promising strategy to reduce Campylobacter spp. in poultry may be done due to the beneficial properties of probiotic bacteria and their bacteriocins. In this study, inhibition spectrum/activity of different enterocins was evaluated against Campylobacter spp. (isolated from different poultry) to indicate further practical use of enterocins. Enterocins are antimicrobial proteinaceous substances produced mostly by enterococci. Feces from broiler chickens (10), laying hens (47), ostriches (140), and ducks (40) were screened. Altogether, 23 strains were allotted to the species Campylobacter jejuni and Campylobacter coli using MALDI TOF mass spectrometry and confirmed by genotyping (PCR method). In the feces of ostriches, Campylobacter spp. were not confirmed. Campylobacter spp. isolated from different poultry showed resistance to nalidixic acid, ciprofloxacin, and ampicillin. Interestingly, strains demonstrating antibiotic resistance revealed sensitivity to at least one of the nine enterocins used (except C. coli Kc1, SZ3, and C. jejuni 1/D). Almost 52% strains were inhibited by Ent A (P). Enterocins can therefore be used to prevent or reduce Campylobacter spp.; it is a basis for practical use.
Institute of Animal Physiology , Slovak Academy of Sciences, Košice, Slovak Republic.
Jana
J
Ščerbová
Institute of Animal Physiology , Slovak Academy of Sciences, Košice, Slovak Republic.
Andrea
A
Lauková
D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology*; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D017000:Campylobacter coli / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D002645:Chickens; D002939:Ciprofloxacin / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests; D024901:Drug Resistance, Multiple, Bacterial; D004372:Ducks; D016984:Enterococcus faecium / Q000302:isolation & purification / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005260:Female; D008297:Male; D058889:Molecular Typing; D009268:Nalidixic Acid / Q000494:pharmacology; D018154:Slovakia; D020310:Struthioniformes
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C107799:enterocin A; D009268:Nalidixic Acid; D002939:Ciprofloxacin; D000667:Ampicillin