Isolation of the Novel Chiral Insecticide Paichongding (IPP) Degrading Strains and Biodegradation Pathways of RR/SS-IPP and SR/RS-IPP in an Aqueous System.
Chiral insecticide paichongding (IPP) is a member of cis-nitromethylene neonicotinoid insecticides used in China. IPP was the promising replacement for imidacloprid as a result of its higher activity against imidacloprid-resistant insects. Two pairs of enantiomers, RR/SS-IPP and SR/RS-IPP, were separated by preparative high-performance liquid chromatography and employed in an aqueous system to investigate their biodegradation process. In this study, the strains G1-13/G1-14 and G2-19 with effective IPP degrading capability were isolated from agricultural soils. G1-14 was mutated from G1-13 by ultraviolet light exposure. Sequence alignment of 16S rRNA proved that these three strains belonged to the genus of Sphingobacterium. The degradation rate of RR/SS-IPP by Sphingobacterium sp. G1-13 and G1-14 reached 13 and 30% within 6 and 4 days, respectively. The degradation rate of SR/RS-IPP by Sphingobacterium sp. G2-19 could reach 35% within 5 days. Degradation intermediates (I1-I6) of enantiomers were detected, and two possible biodegradation pathways were proposed on the basis of the identification of metabolites.
Laboratory of Applied Microbiology and Biotechnology, School of Pharmaceutical Engineering & Life Science, Changzhou University , Changzhou, Jiangsu 213164, People's Republic of China.
Jing
J
Wang
Laboratory of Applied Microbiology and Biotechnology, School of Pharmaceutical Engineering & Life Science, Changzhou University , Changzhou, Jiangsu 213164, People's Republic of China.
Jie
J
Chen
Laboratory of Applied Microbiology and Biotechnology, School of Pharmaceutical Engineering & Life Science, Changzhou University , Changzhou, Jiangsu 213164, People's Republic of China.
Wenjuan
W
Zhu
Laboratory of Applied Microbiology and Biotechnology, School of Pharmaceutical Engineering & Life Science, Changzhou University , Changzhou, Jiangsu 213164, People's Republic of China.
Jiangtao
J
Ma
Laboratory of Applied Microbiology and Biotechnology, School of Pharmaceutical Engineering & Life Science, Changzhou University , Changzhou, Jiangsu 213164, People's Republic of China.
Yan
Y
Rong
Laboratory of Applied Microbiology and Biotechnology, School of Pharmaceutical Engineering & Life Science, Changzhou University , Changzhou, Jiangsu 213164, People's Republic of China.
Zhiqiang
Z
Cai
D053961:Azabicyclo Compounds / Q000737:chemistry* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D007306:Insecticides / Q000737:chemistry / Q000378:metabolism*; D016296:Mutagenesis; D011725:Pyridines / Q000737:chemistry* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S; D045249:Sphingobacterium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism
D053961:Azabicyclo Compounds; D007306:Insecticides; D011725:Pyridines; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; C588015:paichongding
Sphingobacterium; aqueous system; biodegradation pathways; degradation intermediates; paichongding