Simultaneous analysis of neutral monosaccharides, fatty acids and cholesterol as biomarkers from a drop of blood.
OBJECTIVE
We have developed a method for simultaneous monitoring of more biomarkers from three different classes of compounds by simultaneous analysis of neutral monosaccharides, fatty acids (FAs) and cholesterol as their per-O-methylated derivatives from a drop of blood by GC-MS. This work is a development of our previous results about analysis of neutral monosaccharides from a drop of blood.


RESULTS
The simultaneous per-O-methylation was obtained by methylation in one step with methyl iodide and NaOH in DMSO. The per-O-methylated derivatives were separated in one chromatogram. The quantitative analysis was reproducible for five monosaccharides, 22 FAs and cholesterol. The results of this method were compared with those of the enzymatic methods using commercial kits.


CONCLUSIONS
This method can avoid the saponification of the FA methyl esters and can analyze for the first time simultaneously neutral monosaccharides, FAs and cholesterol from a drop of blood.
Department of Chemistry, West University of Timisoara, Strada Pestalozzi 16, RO-300115 Timisoara, Romania.
Ionel
I
Ciucanu
Department of Chemistry, West University of Timisoara, Strada Pestalozzi 16, RO-300115 Timisoara, Romania.
Luminiţa
L
Pilat
Faculty of Medicine, 'Victor Babes' University of Medicine & Pharmacy of Timisoara, Piaţa E. Murgu 2, RO-300041 Timisoara, Romania.
Cristian I
CI
Ciucanu
Faculty of Medicine, 'Victor Babes' University of Medicine & Pharmacy of Timisoara, Piaţa E. Murgu 2, RO-300041 Timisoara, Romania.
Eugen
E
Şişu
Faculty of Medicine, 'Victor Babes' University of Medicine & Pharmacy of Timisoara, Piaţa E. Murgu 2, RO-300041 Timisoara, Romania.
Victor
V
Dumitraşcu
D015415:Biomarkers / Q000097:blood*; D002784:Cholesterol / Q000097:blood*; D005227:Fatty Acids / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006847:Hydrocarbons, Iodinated / Q000737:chemistry; D008745:Methylation; D009005:Monosaccharides / Q000097:blood*; D015203:Reproducibility of Results
D015415:Biomarkers; D005227:Fatty Acids; D006847:Hydrocarbons, Iodinated; D009005:Monosaccharides; D002784:Cholesterol; C014055:methyl iodide
blood; cholesterol; disease-related biomarkers; fatty acids; monosaccharides; simultaneous analysis; simultaneous monitoring of multiple diseases