HPV strain distribution in patients with genital warts in a female population sample.
The incidence of human papillomavirus (HPV) in the human cancer domain is still a subject of intensive study. In this study, we examined cervical swab samples from 713 females with genital warts, and tested the samples for high- and low-risk genital HPV. HPV genotyping was assessed using a Genotyping test that detects HPV by the amplification of target DNA using polymerase chain reaction and nucleic acid hybridization. In total, we detected 37 anogenital HPV DNA genotypes [6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 45, 51, 52, 53, 54, 55, 56, 58, 59, 61, 62, 64, 66, 67, 68, 69, 70, 71, 72, 73 (MM9), 81, 82 (MM4), 83 (MM7), 84 (MM8), IS39 and CP6108] and investigated the incidence of these genotypes in the patients with genital warts. We found differences in the distribution of high-/low-risk strains and the incidence of high-risk strains was found to occur mainly in females under 35 years of age. The data from our study suggest that a detailed oral, rectal and genital identification of high-risk strains should be performed to visualize the entire pattern of possible triggers of carcinogenesis.
Dermatology Research Laboratory, 'Carol Davila' University of Medicine and Pharmacy, Bucharest 050474, Romania; Department of Dermatology, Carol Medical Center, Bucharest 020915, Romania.
Daniel
D
Boda
Department of Immunology, 'Victor Babes' National Institute of Pathology, Bucharest 050096, Romania; Faculty of Biology, University of Bucharest, Bucharest 050095, Romania.
Monica
M
Neagu
Department of Immunology, 'Victor Babes' National Institute of Pathology, Bucharest 050096, Romania.
Carolina
C
Constantin
The Institute for World Economy, Bucharest 050711, Romania.
Razvan Nicolae
RN
Voinescu
Dermatology Research Laboratory, 'Carol Davila' University of Medicine and Pharmacy, Bucharest 050474, Romania.
Constantin
C
Caruntu
Department of Pathology, 'Carol Davila' University of Medicine and Pharmacy, Colentina University Hospital, Bucharest 020125, Romania.
Sabina
S
Zurac
Laboratory of Clinical Virology, Medical School, University of Crete, Heraklion 71409, Greece.
Demetrios A
DA
Spandidos
Research Laboratory of Clinical Pharmacology and Pharmacogenomics, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens 157 71, Greece.
Nikolaos
N
Drakoulis
Department of Forensic Sciences and Toxicology, Medical School, University of Crete, Heraklion 71003, Greece.
Dimitrios
D
Tsoukalas
Department of Forensic Sciences and Toxicology, Medical School, University of Crete, Heraklion 71003, Greece.
Aristides M
AM
Tsatsakis
HPV genotyping; carcinogenesis; cervical cancer; genital warts