Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers.
BACKGROUND
The molecular mechanism underlying broiler fat deposition is still poorly understood.


METHODS
Currently, we used two-dimensional gel electrophoresis (2DE) to identify differentially expressed proteins in abdominal adipose tissues of birds at 4 week of age derived from Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF).


RESULTS
Thirteen differentially expressed protein spots were screened out and identified by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The protein spots were matched to thirteen proteins by searching against the NCBInr database. These identified proteins were apolipoprotein A-I (Apo A-I), cytokeratin otokeratin, ATP synthase subunit alpha, peptidyl-prolyl cis-trans isomerase FKBP4 (PPIase FKBP4), aspartate aminotransferase, carbonic anhydrase II (CA-II), prostaglandin-H2 D-isomerase precursor, fibrinogen alpha chain, lamin-A (LMNA), superoxide dismutase [Mn] (MnSOD), heat shock protein beta-1 (HSPβ1) and two predicted proteins. These differentially expressed proteins are involved mainly in lipid metabolism, amino acid metabolism, signal transduction, energy conversion, antioxidant, and cytoskeleton. Differential expression of Apo A-I, PPIase FKBP4, and cytokeratin otokeratin proteins were further confirmed by Western blot analysis. Quantitative real-time RT-PCR analyses showed that, of these 13 differentially expressed proteins, only PPIase FKBP4 and cytokeratin otokeratin were differentially expressed at mRNA level between the two lines.


CONCLUSIONS
Our results have provided further information for understanding the basic genetics control of growth and development of broiler adipose tissue.
Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key Laboratory of Animal Genetics, Breeding and Reproduction of Education Department of Heilongjiang Province, College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030 Heilongjiang China.
Chun-Yan
CY
Wu
Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key Laboratory of Animal Genetics, Breeding and Reproduction of Education Department of Heilongjiang Province, College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030 Heilongjiang China.|Weifang Academy of Agricultural Sciences, Weifang, 261071 Shandong China.
Yuan-Yuan
YY
Wu
Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key Laboratory of Animal Genetics, Breeding and Reproduction of Education Department of Heilongjiang Province, College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030 Heilongjiang China.
Chun-Dong
CD
Liu
Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key Laboratory of Animal Genetics, Breeding and Reproduction of Education Department of Heilongjiang Province, College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030 Heilongjiang China.
Yu-Xiang
YX
Wang
Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key Laboratory of Animal Genetics, Breeding and Reproduction of Education Department of Heilongjiang Province, College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030 Heilongjiang China.
Wei
W
Na
Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key Laboratory of Animal Genetics, Breeding and Reproduction of Education Department of Heilongjiang Province, College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030 Heilongjiang China.
Ning
N
Wang
Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key Laboratory of Animal Genetics, Breeding and Reproduction of Education Department of Heilongjiang Province, College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030 Heilongjiang China.
Hui
H
Li
Abdominal adipose tissue; Broiler; Differentially expressed protein; Proteomics