Effects of acetaminophen and mannitol on crush injuries in rats: An experimental study.
BACKGROUND
The present objective was to evaluate effects of acetaminophen and mannitol on renal function and histopathology in crush injuries.


METHODS
Thirty-six rats weighing 370-400 g each were used. No surgery was performed on the first (control) group. The gastrocnemius muscle regions of each rat in the remaining 5 groups were compressed for 2 or 24 hours. In the 4th group, 100 mg/kg acetaminophen was intraperitoneally administered. In the 5th group, 1 g/kg mannitol was administered. In the 6th group, 100 mg/kg acetaminophen and 1 g/kg mannitol were administered.


RESULTS
No statistically significant differences were observed among the treatment groups in terms of sodium, potassium, alanine aminotransferase (ALT), and average creatinine clearance values. Hydropic degeneration, tubular necrosis, presence of immunoperoxidase and myoglobin, tubulus epithelial cell degeneration, and presence of PAS-dyed material in tubular lumen was more prominently decreased in the acetaminophen group than the mannitol group. Improvement was observed in the group that was administered both drugs, compared to the mannitol-only group, though findings were still worse than those of the group administered acetaminophen only.


CONCLUSIONS
In crush injuries, acetaminophen improves histopathological renal damage better than mannitol. When used in conjunction with mannitol, the toxic effect of acetaminophen on the liver is decreased.
Department of Emergency, Yeditepe University Faculty of Medicine, İstanbul-Turkey.
Mustafa Ferudun
MF
Çelikmen
Department of Emergency, Yeditepe University Faculty of Medicine, İstanbul-Turkey.
Sezgin
S
Sarıkaya
İstanbul University Health Sciences Faculty, İstanbul-Turkey. dogacniyazi@gmail.com.
Doğaç Niyazi
DN
Özüçelik
Department of Nephrology, İstanbul University Faculty of Medicine, İstanbul-Turkey.
Mehmet Şükrü
MŞ
Sever
Department of Emergency Medicine, İstanbul Medeniyet University Faculty of Medicine, İstanbul-Turkey.
Kurtuluş
K
Açıksarı
Department of Internal Medicine, Haydarpaşa Numune Training and Research Hospital, İstanbul-Turkey.
Deniz Maktav
DM
Çelikmen
Department of Emergency, Bakırköy Dr. Kadi Konuk Training and Research Hospital, İstanbul-Turkey.
Mustafa
M
Yazıcıoğlu
Department of Emergency, Yeditepe University Faculty of Medicine, İstanbul-Turkey.
Ali
A
Kandemir
Department of Emergency, Bakırköy Dr. Kadi Konuk Training and Research Hospital, İstanbul-Turkey.
Halil
H
Doğan
Department of Emergency, Okmeydanı Training and Research Hospital, İstanbul-Turkey.
Barış Murat
BM
Ayvacı
Department of Emergency, Haseki Training and Research Hospital, İstanbul-Turkey.
Derya
D
Özaşır Abuşka
Department of Emergency, İstanbul Training and Research Hospital, İstanbul-Turkey.
Sıla
S
Sadıllıoğlu
D000082:Acetaminophen / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D058186:Acute Kidney Injury / Q000097:blood / Q000517:prevention & control*; D000410:Alanine Transaminase / Q000097:blood; D018712:Analgesics, Non-Narcotic / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D003444:Crush Syndrome / Q000097:blood / Q000188:drug therapy*; D004195:Disease Models, Animal; D004234:Diuretics, Osmotic / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D007668:Kidney / Q000187:drug effects; D008297:Male; D008353:Mannitol / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley
D018712:Analgesics, Non-Narcotic; D004234:Diuretics, Osmotic; D000082:Acetaminophen; D008353:Mannitol; D000410:Alanine Transaminase