Quantification of the viscoelasticity of the bond of biotic and abiotic particles adhering to solid-liquid interfaces using a window-equipped quartz crystal microbalance with dissipation.
The quartz-crystal-microbalance-with-dissipation (QCM-D) has become a powerful tool for studying the bond viscoelasticity of biotic and abiotic colloidal particles adhering to substratum surfaces. A window-equipped QCM-D allows high-throughput analysis of the average bond viscoelasticity, measuring over 106particles simultaneously in one single experiment. Other techniques require laborious analyses of individual particles. In this protocol, the quantitative derivation of the spring-constant and drag-coefficient of the bond between adhering colloidal particles and substratum surfaces using QCM-D is explained for bacteria and silica particles, using the particle-mass derived for validation. Bond viscoelasticity is calculated using a coupled resonator model, paying special attention to the protocol for mathematical fitting needed to obtain reliable quantitative output. Knowledge of the viscoelasticity of the bond between colloidal particles and substratum surfaces facilitates development of new strategies to detach adhering particles from or retain them on a surface.
University of Groningen and University, Medical Center Groningen, Department of Biomedical Engineering, P.O. Box 196, 9700 AD, Groningen, The Netherlands.
Rebecca
R
van der Westen
University of Groningen and University, Medical Center Groningen, Department of Biomedical Engineering, P.O. Box 196, 9700 AD, Groningen, The Netherlands.
Henny C
HC
van der Mei
University of Groningen, Faculty of Mathematics and Natural Sciences, Computational Physics, Zernike Institute for Advanced Materials, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Hans
H
De Raedt
University of Groningen and University, Medical Center Groningen, Department of Biomedical Engineering, P.O. Box 196, 9700 AD, Groningen, The Netherlands; McGill University, Montreal, Department of Chemical Engineering, Quebec H3A 2B2, Canada.
Adam L J
ALJ
Olsson
University of Groningen and University, Medical Center Groningen, Department of Biomedical Engineering, P.O. Box 196, 9700 AD, Groningen, The Netherlands.
Henk J
HJ
Busscher
University of Groningen and University, Medical Center Groningen, Department of Biomedical Engineering, P.O. Box 196, 9700 AD, Groningen, The Netherlands. Electronic address: p.k.sharma@umcg.nl.
Prashant K
PK
Sharma
D001422:Bacterial Adhesion; D004548:Elasticity; D058525:Quartz Crystal Microbalance Techniques; D014783:Viscosity
Adhesion; Bacteria; Bond; Bond stiffness; Kelvin-Voigt; Particles; Quartz crystal microbalance; Viscoelasticity