Antimicrobial efficacy of sequentially applied eugenol against food spoilage micro-organisms.
OBJECTIVE
Compare the survival behaviour of food spoilage micro-organisms treated with sequential doses or all at once treatments of eugenol.


RESULTS
Staphylococcus carnosus, Listeria innocua, Escherichia coli and Pseudomonas fluorescens were exposed to a minimal lethal concentration (MLC) initially, or to half doses over time, with first half dose applied immediately and a second half dose applied after 3, 4, 6 and 8 h, of eugenol. Direct plate counts were determined at regular time intervals. Population dynamics were analysed using a combined growth and mortality model. Effect of sequential dosing varied significantly between tested organisms. High antimicrobial efficacy on E. coli K12 was observed regardless of timing of the two doses. Reduced effectiveness was observed against Staph. carnosus and L. innocua the later the second half dose was applied. Complete cell reduction occurred after immediate exposure to full MLC dose, while sequential half doses were bacteriostatic regardless of application times.


CONCLUSIONS
Time in between antimicrobial dose application had substantial impact on effectiveness, attributed to organisms becoming more tolerant.


CONCLUSIONS
This study contributes to the evaluation of encapsulated antimicrobial systems, where antimicrobials are released over time and antimicrobial concentrations may only reach MLC levels after some time.
Department of Food Physics and Meat Science, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.
Y
Y
Manrique
Department of Food Physics and Meat Science, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.
M
M
Gibis
Department of Food Microbiology and Hygiene, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.
H
H
Schmidt
Department of Food Physics and Meat Science, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.
J
J
Weiss
D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D048168:Escherichia coli K12 / Q000187:drug effects; D005054:Eugenol / Q000008:administration & dosage* / Q000494:pharmacology; D005516:Food Microbiology; D005519:Food Preservation; D008087:Listeria / Q000187:drug effects; D011551:Pseudomonas fluorescens / Q000187:drug effects; D013210:Staphylococcus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D005054:Eugenol
essential oils; kinetics; modelling; population dynamics; resistance