Overexpression of CuZnSOD and APX enhance salt stress tolerance in sweet potato.
Abiotic stresses cause accumulation of reactive oxygen species (ROS) in plants, CuZnSOD and APX are first line defenses against ROS caused by oxidative stress. In this study, CuZnSOD and APX were transferred into salt sensitive sweet potato (cv. Xushu 55-2) under control of stress inducible SWPA2 promoter and tolerance to salt stress was evaluated. When 100 mM NaCl was used to treat stem cuttings, transgenic plants showed enhanced tolerance compared to wild type (WT) plants. Rooting was significantly retarded in WT plants whereas all transgenic plants had significantly enhanced root growth under salt stress. Integration of SOD gene was confirmed by southern blot analysis, and the copy number ranged from 1 to 3. The expression levels of CuZnSOD and APX in transgenic plants were significantly increased up to 13.3 and 7.8 folds to WT under salinity conditions, respectively. SOD and APX activity and ROS staining showed enzyme activities of transgenic plants were increased under salt stress. These results show that CuZnSOD and APX have important roles in enhancing the salt tolerance of sweet potato.
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Key Laboratory for Biology and Genetic Breeding of Sweetpotato (Xuzhou), Ministry of Agriculture/Jiangsu Xuzhou Sweetpotato Research Center, 221131, People's Republic of China.
Hui
H
Yan
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Key Laboratory for Biology and Genetic Breeding of Sweetpotato (Xuzhou), Ministry of Agriculture/Jiangsu Xuzhou Sweetpotato Research Center, 221131, People's Republic of China. Electronic address: instrong@163.com.
Qiang
Q
Li
Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Oun-dong 52, Yusong-gu, Daejeon, 305-806, Republic of Korea.
Sung-Chul
SC
Park
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Key Laboratory for Biology and Genetic Breeding of Sweetpotato (Xuzhou), Ministry of Agriculture/Jiangsu Xuzhou Sweetpotato Research Center, 221131, People's Republic of China.
Xin
X
Wang
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Key Laboratory for Biology and Genetic Breeding of Sweetpotato (Xuzhou), Ministry of Agriculture/Jiangsu Xuzhou Sweetpotato Research Center, 221131, People's Republic of China.
Ya-Ju
YJ
Liu
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Key Laboratory for Biology and Genetic Breeding of Sweetpotato (Xuzhou), Ministry of Agriculture/Jiangsu Xuzhou Sweetpotato Research Center, 221131, People's Republic of China.
Yun-Gang
YG
Zhang
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Key Laboratory for Biology and Genetic Breeding of Sweetpotato (Xuzhou), Ministry of Agriculture/Jiangsu Xuzhou Sweetpotato Research Center, 221131, People's Republic of China.
Wei
W
Tang
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Key Laboratory for Biology and Genetic Breeding of Sweetpotato (Xuzhou), Ministry of Agriculture/Jiangsu Xuzhou Sweetpotato Research Center, 221131, People's Republic of China.
Meng
M
Kou
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Key Laboratory for Biology and Genetic Breeding of Sweetpotato (Xuzhou), Ministry of Agriculture/Jiangsu Xuzhou Sweetpotato Research Center, 221131, People's Republic of China.
Dai-Fu
DF
Ma
D060387:Ascorbate Peroxidases / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D027723:Ipomoea batatas / Q000201:enzymology / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism
D017382:Reactive Oxygen Species; D012965:Sodium Chloride; D060387:Ascorbate Peroxidases; D013482:Superoxide Dismutase
APX; CuZnSOD; Salt stress; Sweet potato; Transgenic plants