In vitro disintegration of goat brain cystatin fibrils using conventional and gemini surfactants: Putative therapeutic intervention in amyloidoses.
Many protein misfolding diseases in mammalian system are characterised by the accumulation of protein aggregates in amyloid fibrillar forms. Several therapeutic approaches include reduction in the production of the amyloidogenic form of proteins, increase in the clearance rate of misfolded or aggregated proteins, and direct inhibition of the self-assembly process have been explained. One of the possible remedial treatments for such disorders may be to identify molecules which are capable of either preventing formation of fibrils or disintegrating the formed fibrils. In this work, we have studied the effect of conventional surfactants; sodium dodecylsulphate (SDS), cetyl trimethylammonium bromide (CTAB) and dicationic gemini (16-4-16) surfactant on the disintegration of the goat brain cystatin (GBC) fibrils above their critical micelle concentrations (CMC) using ThT fluorescence, CD, TEM, Congo red and turbidity approaches. The results obtained are significant and showing the best disintegrating potency on GBC fibrils with gemini surfactant. The outcome from this work will aid in the development and/or design of potential inhibitory agents against amyloid deposits associated with amyloid diseases.
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, UP, India.
Waseem Feeroze
WF
Bhat
Department of Chemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, UP, India.
Imtiyaz Ahmad
IA
Bhat
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, UP, India.
Sheraz Ahmad
SA
Bhat
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, UP, India. Electronic address: bilqeesbano99@gmail.com.
Bilqees
B
Bano
D000682:Amyloid / Q000737:chemistry*; D000686:Amyloidosis; D000818:Animals; D001921:Brain; D001923:Brain Chemistry; D015891:Cystatins / Q000737:chemistry*; D006041:Goats; D066263:Protein Aggregation, Pathological; D013501:Surface-Active Agents / Q000737:chemistry*
D000682:Amyloid; D015891:Cystatins; D013501:Surface-Active Agents
CTAB; Gemini surfactant; Goat brain cystatin; SDS