A potential determinant role of adiponectin and receptors for the early embryo development in PCOS patients with obesity hinted by quantitative profiling.
OBJECTIVE
To identify the quantitative profiling of adiponectin and its receptors (AdipoR1, AdipoR2, and T-cadherin) in cumulus cells (CCs) and to evaluate their roles in the early embryo development of polycystic ovary syndrome (PCOS) patients, in part, with obesity.


METHODS
Fifty-five subjects were divided into two groups according to the body mass index. Oocytes were further inseminated and only mature and normal fertilized oocytes (2PN) were included in this research. Real-time PCR and western blot were performed to identify adiponectin and its receptors in CCs.


RESULTS
Adiponectin and receptors were ubiquitously expressed in CCs of PCOS and non-PCOS patients. The level of AdipoR2 in CCs from the oocytes yielding blastocyst after 5/6 days in vitro culture was markedly higher than in those from oocytes could not develop to blastocyst stage after Day 6, for non-obese or obese PCOS patients (0.1647 ± 0.0161 versus 0.0783 ± 0.0385, 0.1948 ± 0.0307 versus 0.1057 ± 0.0236, respectively, p < 0.05). In addition, only in patients with PCOS and concurrent obesity the AdipoR1 in CCs was considerably increased in CC-B+compared with CC-B-subgroup (0.5162 ± 0.0371 versus 0.2448 ± 0.0333, p < 0.01).


CONCLUSIONS
The development of early embryo was associated with the up-regulation of AdipoR1 and AdipoR2 in PCOS patients. Our results suggested that adiponectin could positively modulate embryo development in humans. Further investigations should be carried out to unlock the crucial role that adiponectin plays in embryo development.
a Reproductive Medicine Center, Yantai Yuhuangding Hospital Affiliated to Qingdao University , Yantai , China and.
Ning
N
Zhang
a Reproductive Medicine Center, Yantai Yuhuangding Hospital Affiliated to Qingdao University , Yantai , China and.
Cuifang
C
Hao
a Reproductive Medicine Center, Yantai Yuhuangding Hospital Affiliated to Qingdao University , Yantai , China and.
Xiaoyan
X
Liu
b Biochip Laboratory, Yantai Yuhuangding Hospital Affiliated to Qingdao University , Yantai , China.
Shouxin
S
Zhang
a Reproductive Medicine Center, Yantai Yuhuangding Hospital Affiliated to Qingdao University , Yantai , China and.
Fengrong
F
Zhang
a Reproductive Medicine Center, Yantai Yuhuangding Hospital Affiliated to Qingdao University , Yantai , China and.
Lili
L
Zhuang
a Reproductive Medicine Center, Yantai Yuhuangding Hospital Affiliated to Qingdao University , Yantai , China and.
Dongmei
D
Zhao
D052242:Adiponectin / Q000378:metabolism*; D000328:Adult; D001757:Blastomeres / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism*; D054885:Cumulus Cells / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D006801:Humans; D009765:Obesity / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D054419:Receptors, Adiponectin / Q000378:metabolism*; D015854:Up-Regulation
C499918:ADIPOQ protein, human; C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D052242:Adiponectin; D015820:Cadherins; C099972:H-cadherin; D054419:Receptors, Adiponectin
Adiponectin; adiponectin receptor; cumulus cell; embryo development; polycystic ovary syndrome