Individual strains of Lactobacillus paracasei differentially inhibit human basophil and mouse mast cell activation.
BACKGROUND
The microbiota controls a variety of biological functions, including immunity, and alterations of the microbiota in early life are associated with a higher risk of developing allergies later in life. Several probiotic bacteria, and particularly lactic acid bacteria, were described to reduce both the induction of allergic responses and allergic manifestations. Although specific probiotic strains were used in these studies, their protective effects on allergic responses also might be common for all lactobacilli.


METHODS
To determine whether allergic effector cells inhibition is a common feature of lactobacilli or whether it varies among lactobacilli strains, we compared the ability of 40 strains of the same Lactobacillus paracasei species to inhibit IgE-dependent mouse mast cell and human basophil activation.


RESULTS
We uncovered a marked heterogeneity in the inhibitory properties of the 40 Lactobacillus strains tested. These segregated into three to four clusters depending on the intensity of inhibition. Some strains inhibited both mouse mast cell and human basophil activation, others strains inhibited only one cell type and another group induced no inhibition of activation for either cell type.


CONCLUSIONS
Individual Lactobacillus strains of the same species differentially inhibit IgE-dependent activation of mouse mast cells and human basophils, two cell types that are critical in the onset of allergic manifestations. Although we failed to identify specific bacterial genes associated with inhibition by gene-trait matching analysis, our findings demonstrate the complexity of the interactions between the microbiota and the host. These results suggest that some L. paracasei strains might be more beneficial in allergies than others strains and provide the bases for a rational screening of lactic acid bacteria strains as next-generation probiotics in the field of allergy.
Unité d'Allergologie Moléculaire & Cellulaire Institut Pasteur Paris France.
Lydie
L
Cassard
Unité d'Allergologie Moléculaire & Cellulaire Institut Pasteur Paris France.
Ana Inés
AI
Lalanne
Danone Nutricia Research Palaiseau France.
Peggy
P
Garault
Soladis Lyon France.
Aurélie
A
Cotillard
Danone Nutricia Research Palaiseau France.
Christian
C
Chervaux
NIZO Food Research Kluyver Centre for Genomics of Industrial Fermentation Ede The Netherlands.
Michiel
M
Wels
Danone Nutricia Research Palaiseau France.
Tamara
T
Smokvina
Unité d'Allergologie Moléculaire & Cellulaire Institut Pasteur Paris France.
Marc
M
Daëron
Danone Nutricia Research Palaiseau France.
Raphaëlle
R
Bourdet-Sicard
Lactobacillus; basophil; mast cell