DNA Antenna Tile-Associated Deoxyribozyme Sensor with Improved Sensitivity.
Some natural enzymes increase the rate of diffusion-limited reactions by facilitating substrate flow to their active sites. Inspired by this natural phenomenon, we developed a strategy for efficient substrate delivery to a deoxyribozyme (DZ) catalytic sensor. This resulted in a three- to fourfold increase in sensitivity and up to a ninefold improvement in the detection limit. The reported strategy can be used to enhance catalytic efficiency of diffusion-limited enzymes and to improve sensitivity of enzyme-based biosensors.
Chemistry Department, University of Central Florida, 4000 Central Florida Blvd, Orlando, FL, 32816-2366, USA.|Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 6900 Lake Nona Blvd., Orlando, FL, 32827, USA.
Amanda J
AJ
Cox
Chemistry Department, University of Central Florida, 4000 Central Florida Blvd, Orlando, FL, 32816-2366, USA.|Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 6900 Lake Nona Blvd., Orlando, FL, 32827, USA.
Hillary N
HN
Bengtson
Chemistry Department, University of Central Florida, 4000 Central Florida Blvd, Orlando, FL, 32816-2366, USA.
Yulia V
YV
Gerasimova
Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 6900 Lake Nona Blvd., Orlando, FL, 32827, USA.
Kyle H
KH
Rohde
Chemistry Department, University of Central Florida, 4000 Central Florida Blvd, Orlando, FL, 32816-2366, USA. Dmitry.Kolpashchikov@ucf.edu.|Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 6900 Lake Nona Blvd., Orlando, FL, 32827, USA. Dmitry.Kolpashchikov@ucf.edu.|National Center for Forensic Science, University of Central Florida, 12354 Research Pkwy. Suite 225, Orlando, FL, 32826, USA. Dmitry.Kolpashchikov@ucf.edu.
Dmitry M
DM
Kolpashchikov
D055162:Biocatalysis; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*
D021881:DNA, Catalytic; D004247:DNA
DNA crossover tile; deoxyribozymes; diffusion-limited reactions; mycobacterium tuberculosis; split hybridization probes