MacoNPV baculovirus midgut-specific gene expression during infection of the bertha armyworm, Mamestra configurata.
Baculoviruses have two forms, occlusion derived virus (ODV) which is responsible for primary infection in host midgut tissue and budded virus (BV), which infects all other host tissues during secondary infection. This study examined the primary infection by ODV of midgut cells of bertha armyworm Mamestra configurata fourth instar larvae and measured the expression of viral genes over a time course of infection. Both digital PCR and RNA sequencing methods showed the profile of transcription to be different from those produced by AcMNPV BV infection of in vitro cell cultures. This included having unique collections of genes expressed early, as well as much greater late gene expression of p6.9 and much reduced expression of polh and p10. These differences likely reflect characteristics unique to the critical step of in vivo midgut cell infection, and provide insights into the processes that regulate viral gene expression in different host tissues.
London Research and Development Centre, AAFC, London, ON, Canada. Electronic address: Cam.Donly@agr.gc.ca.
B Cameron
BC
Donly
London Research and Development Centre, AAFC, London, ON, Canada.
Emine
E
Kaplanoglu
Summerland Research and Development Centre, AAFC, Summerland, BC, Canada.
David A
DA
Theilmann
Saskatoon Research and Development Centre, AAFC, Saskatoon, SK, Canada.
Doug
D
Baldwin
Saskatoon Research and Development Centre, AAFC, Saskatoon, SK, Canada.
Edyta
E
Sieminska
Saskatoon Research and Development Centre, AAFC, Saskatoon, SK, Canada.
Dwayne D
DD
Hegedus
Saskatoon Research and Development Centre, AAFC, Saskatoon, SK, Canada.
Martin A
MA
Erlandson
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D041981:Gastrointestinal Tract / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D059014:High-Throughput Nucleotide Sequencing; D007814:Larva / Q000821:virology; D009036:Moths / Q000821:virology*; D016366:Open Reading Frames; D060888:Real-Time Polymerase Chain Reaction; D014158:Transcription, Genetic; D059467:Transcriptome
Baculovirus; Digital PCR; MacoNPV; Mamestra configurata; Occlusion derived virus; RNA sequencing; Transcriptomics