Investigating the Effects of Systemically Administered Strontium Ranelate on Alveolar Bone Loss Histomorphometrically and Histopathologically on Experimental Periodontitis in Rats.
The aim of this study is to investigate effects of strontium ranelate (SR) on alveolar bone loss (ABL) in rats with experimental periodontitis.



Forty Wistar rats were randomly divided into five groups: 1) control (n = 8); 2) ligated (n = 8); 3) 300 mg/kg SR (SR300, n = 8); 4) 625 mg/kg SR (SR625, n = 8); and 5) 900 mg/kg SR (SR900, n = 8). To create experimental periodontitis, 4/0 silk ligatures were inserted submarginally around first molars at the right mandible. After 11 days, rats were sacrificed. ABL was calculated by measuring cemento-enamel junction and alveolar crest distance. Interleukin (IL)-1β, osteoprotegerin (OPG), and bone-specific alkaline phosphatase (BALP) serum levels were determined by enzyme-linked immunosorbent assay. Histopathologic analysis was used to evaluate inflammatory cell infiltration, numbers of osteoblasts and osteoclasts, and receptor activator of nuclear factor-kappa B ligand (RANKL) activity.



ABL was significantly lower in SR900 group than in the ligated group (P <0.05). Osteoclast numbers in ligated group were significantly higher than in the control, SR300, and SR900 groups (P <0.05). In ligated, SR625, and SR900 groups, significantly higher osteoblast numbers were detected than in control group (P <0.05). Osteoblast numbers in SR625 group were significantly higher than in the SR300 group (P <0.05). RANKL activities in SR900 and control groups were close to each other (P >0.05). Serum IL-1β, OPG, and BALP levels revealed no significant difference (P >0.05).



It can be concluded that SR can reduce RANKL activity and osteoclast numbers, as well as ABL.
Department of Periodontology, Faculty of Dentistry, Cumhuriyet University, Sivas, Turkey.
Nebi Cansın
NC
Karakan
Department of Periodontology, Faculty of Dentistry, Cumhuriyet University, Sivas, Turkey.
Aysun
A
Akpınar
Department of Pathology, Faculty of Medicine, Cumhuriyet University.
Fahrettin
F
Göze
Department of Microbiology, Faculty of Medicine, Cumhuriyet University.
Ömer
Ö
Poyraz
D000469:Alkaline Phosphatase / Q000378:metabolism; D016301:Alveolar Bone Loss / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D010010:Osteoclasts / Q000378:metabolism; D053244:Osteoprotegerin / Q000378:metabolism; D010518:Periodontitis / Q000473:pathology / Q000517:prevention & control*; D053245:RANK Ligand / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013876:Thiophenes / Q000008:administration & dosage / Q000494:pharmacology*
D015415:Biomarkers; D053583:Interleukin-1beta; D053244:Osteoprotegerin; D053245:RANK Ligand; D013876:Thiophenes; C081587:strontium ranelate; D000469:Alkaline Phosphatase
Osteoblasts; osteoclasts; pathology; periodontal diseases; periodontitis; strontium