How does the supernatant of Lactobacillus acidophilus affect the proliferation and differentiation activities of rat bone marrow-derived stromal cells?
Low proliferation rate and unwanted differentiation of bone marrow-derived stromal cells (rBMSCs) during the frequent passages have limited the use of such cells in clinical cell therapy. Recently, the researchers have focused on the effects of the components produced by some bacteria on proliferation of the stem cells. In this study, we discussed the possible effects of the Lactobacillus acidophilus supernatant on proliferation and differentiation of the rBMSCs. For this aim, the cells were isolated from rat bone marrow, characterized by culturing on tissue specific differentiation media and stained. The cells (passage two) were treated with different concentrations of the L. acidophilus supernatant (0, 0.1, 0.3, 0.9, 3, 9 and 30 &mgr;l/ml) for 14 days. The proliferation and differentiation capacity of the cells were then determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT assay) and tissue specific staining. The results showed a positive effect of the supernatant on the cell proliferation in 3 and 9 &mgr;l/ml concentrations, while did not affect the differentiation capacity of the rBMSCs. The current study strongly suggests the L. acidophilus supernatant as an alternative material that could be added to the media with aim of improvement in the proliferation rate of the rBMSCs without affecting their differentiation capacity.
Iran University of Medical Sciences Cellular and Molecular Research Center Tehran Iran.
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Samadikuchaksaraei
Shahid Beheshti University of Medical Sciences Biotechnology Department, School of Advanced Technologies in Medicine Tehran Iran.
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Gholipourmalekabadi
Tehran University of Medical Sciences Department of Medical Laboratory Sciences, School of Allied Medical Sciences Tehran Iran mostafasaberian@gmail.com.
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Saberian
Pasteur Institute of Iran Department of Immunology Tehran Iran.
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Abdollahpour Alitappeh
Shahid Rajaei Cardiovascular Medical and Research Center Department of Critical Care Nursing Tehran Iran.
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Shahidi Delshad
D050156:Adipogenesis; D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D007779:Lactobacillus acidophilus / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D017207:Rats, Sprague-Dawley