MicroRNA-593-3p regulates insulin-promoted glucose consumption by targeting Slc38a1 and CLIP3.
Insulin plays an important role in the regulation of glucose metabolism. However, the molecular mechanisms involved are not entirely clarified. In this context, we found that miR-593-3p negatively regulates insulin-regulated glucose metabolism in hepatocellular carcinoma HepG2 and Bel7402 cells. We then identified Slc38a1 and CLIP3 as novel targets of miR-593-3p. Further studies demonstrated that Slc38a1 and CLIP3 mediate insulin-regulated glucose metabolism. Interestingly, we also demonstrated that miR-593-3p expression was negatively associated with Slc38a1 and CLIP3 expression in insulin-treated HepG2 cells, and insulin-induced Slc38a1 and CLIP3 expression via downregulation of miR-593-3p. Taken together, this study indicates that inhibition of miRNA-593-3p by insulin promotes glucose metabolism through the regulation of Slc38a1 and CLIP3 expression, and provides a new insight into the role and mechanism of insulin-induced glycolysis.
Department of Otorhinolaryngology - Head and Neck SurgeryRenmin Hospital of Wuhan University, Wuhan, China.
Xilin
X
Yang
Department of Otorhinolaryngology - Head and Neck SurgeryRenmin Hospital of Wuhan University, Wuhan, China taozezhang@hotmail.com zyzhu@whu.edu.cn.
Zezhang
Z
Tao
Department of Plastic SurgeryRenmin Hospital of Wuhan University, Wuhan, China taozezhang@hotmail.com zyzhu@whu.edu.cn.
Zhanyong
Z
Zhu
Department of Otorhinolaryngology - Head and Neck SurgeryRenmin Hospital of Wuhan University, Wuhan, China.
Hua
H
Liao
Department of Plastic SurgeryRenmin Hospital of Wuhan University, Wuhan, China.
Yueqiang
Y
Zhao
Department of Plastic SurgeryRenmin Hospital of Wuhan University, Wuhan, China.
Huajun
H
Fan
D020413:3' Untranslated Regions; D026924:Amino Acid Transport System A / Q000235:genetics*; D000818:Animals; D020029:Base Pairing; D002460:Cell Line; D049109:Cell Proliferation; D004734:Energy Metabolism / Q000235:genetics; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism*; D022781:Hepatocytes; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics
D020413:3' Untranslated Regions; D026924:Amino Acid Transport System A; D007328:Insulin; C577650:MIRN593 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C445761:SLC38A1 protein, human; D019344:Lactic Acid; D005947:Glucose
CLIP3; Slc38a1; insulin; miR-593-3p