Use of bacterial whole-genome sequencing to understand and improve the management of invasive Staphylococcus aureus infections.
Management of invasive Staphylococcus aureus infections is complex. Dramatic improvements in bacterial whole genome sequencing (WGS) offer new opportunities for personalising the treatment of S. aureus infections. Areas covered: We address recent achievements in S. aureus genomics, describe genetic determinants of antibiotic resistance and summarise studies that have defined molecular characteristics associated with risk and outcome of S. aureus invasive infections. Potential clinical use of WGS for resistance prediction, infection outcome stratification and management of persistent /relapsing infections is critically discussed. Expert commentary: WGS is not only providing invaluable information to track the emergence and spread of important S. aureus clones, but also allows rapid determination of resistance genotypes in the clinical environment. An evolving opportunity is to infer clinically important outcomes and optimal therapeutic approaches from widely available S. aureus genome data, with the goal of individualizing management of invasive S. aureus infections.
a Microbiological Diagnostic Unit Public Health Laboratory , Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne , Australia.|b Infectious Diseases Service , Department of Medicine, Lausanne University Hospital , Lausanne , Switzerland.
Stefano G
SG
Giulieri
c Infectious Diseases Department , Austin Health , Heidelberg , Australia.
Natasha E
NE
Holmes
d Doherty Applied Microbial Genomics , Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne , Australia.|e Department of Microbiology and Immunology , The University of Melbourne at the Peter Doherty Institute for Infection and Immunity , Melbourne , Australia.
Timothy P
TP
Stinear
a Microbiological Diagnostic Unit Public Health Laboratory , Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, University of Melbourne , Melbourne , Australia.|c Infectious Diseases Department , Austin Health , Heidelberg , Australia.|e Department of Microbiology and Immunology , The University of Melbourne at the Peter Doherty Institute for Infection and Immunity , Melbourne , Australia.
Benjamin P
BP
Howden
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D015373:Bacterial Typing Techniques; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D006801:Humans; D017720:Molecular Epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics
D000900:Anti-Bacterial Agents; D037521:Virulence Factors
Staphylococcus aureus; bacteremia; resistance; virulence; whole genome sequencing