Development and Evaluation of the Protective Efficacy of Novel Marek's Disease Virus Rispens Vector Vaccines Against Infectious Bursal Disease.
Infectious bursal disease (IBD) is a major disease affecting the poultry industry and is caused by infection with IBD virus (IBDV). To develop a novel vaccine to prevent IBD in chickens, recombinant Marek's disease virus Rispens viruses carrying the VP2 gene of IBDV driven by five different promoters (Rispens/IBD) were constructed using homologous recombination and a bacterial artificial chromosome (BAC). Rispens/IBD driven by the chicken beta-actin (Bac) promoter (Rispens/Bac-IBD), Rous sarcoma virus promoter, or simian virus 40 promoter were administered to 1-day-old SPF chicks, and the protective efficacy against IBDV was evaluated by challenging chicks with virulent IBDV. As a result, Rispens/Bac-IBD showed the best protection (87%). Next, we constructed the virus driven by the Bac-derived Coa5 promoter (Rispens/Coa5-IBD) for a secondary in vivo trial using commercial layer chickens since Rispens/Bac-IBD was thought to be genetically unstable. Rispens/Coa5-IBD showed stability in vitro and exhibited better antibody production and protection during challenge against virulent IBDV at both 5 (95%) and 7 wk of age (91%) compared with that of Rispens/Bac-IBD (90% at 5 wk of age and 84% at 7 wk of age). Thus, Rispens/Coa5-IBD may be a novel promising vaccine against IBD and virulent Marek's disease.
Ceva Animal Health (Japan Campus), 1-6 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Yukari
Y
Ishihara
Ceva Animal Health (Japan Campus), 1-6 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Motoyuki
M
Esaki
Ceva Animal Health (Japan Campus), 1-6 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Shuji
S
Saitoh
Ceva Animal Health (Japan Campus), 1-6 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Takanori
T
Sato
Ceva Animal Health (Japan Campus), 1-6 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Atsushi
A
Yasuda
D000818:Animals; D018175:Birnaviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D002645:Chickens; D026002:Herpesvirus 1, Meleagrid / Q000276:immunology; D007243:Infectious bursal disease virus / Q000276:immunology*; D008380:Marek Disease / Q000517:prevention & control; D022501:Marek Disease Vaccines / Q000276:immunology; D011201:Poultry Diseases / Q000517:prevention & control*; D014765:Viral Vaccines / Q000276:immunology*; D014774:Virulence
D022501:Marek Disease Vaccines; D014765:Viral Vaccines
bacterial artificial chromosome; gene expression promoter; infectious bursal disease virus; poultry vaccine