Improving hemocompatibility and accelerating endothelialization of vascular stents by a copper-titanium film.
Bio-inorganic films and drug-eluting coatings are usually used to improve the hemocompatibility and inhibit restenosis of vascular stent; however, above bio-performances couldn't combine together with single materials. In the present study, we reported a simple approach to fabricate a metal film with the aim of imparting the stent with good blood compatibility and accelerating endothelialization. The films with various ratios of Cu and Ti were prepared through the physical vapor deposition. Phase structure and element composition were investigated by X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS), respectively. The releasing volume of copper ion in Cu/Ti film was determined by immersing test. The hemolysis ratio, platelet adhesion and clotting time were applied to evaluate the hemocompatibility. The proliferative behaviors of endothelial cells and smooth muscle cells under certain copper concentration were investigated in vitro and in vivo. Results indicated that copper-titanium films exhibited good hemocompatibility in vitro; however, the increase of Cu/Ti ratio could lead to increasing hemolysis ratio. Endothelial cells displayed more proliferative than smooth muscle cells when the copper concentration was <7.5μg/ml, however both cells tended to apoptosis to some degree when the copper concentration was increased. The complete endothelialization of the film with low copper in vivo was observed at the 2nd week, indicating that the copper-titanium film with the lower copper concentration could promote endothelialization. Therefore, the inorganic copper-titanium film could be potential biomaterials to improve blood compatibility and accelerating endothelialization of vascular stents.
College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China. Electronic address: 99xyxy@163.com.
Hengquan
H
Liu
Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huaiyin 223033, China.
Changjiang
C
Pan
College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China.
Shijie
S
Zhou
College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China.
Junfeng
J
Li
Key Laboratory for Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China.
Nan
N
Huang
Department of Research & Development, Lifetech Scientific (Shenzhen) Co., Ltd, Shenzhen 518057, China.
Lihua
L
Dong
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D056951:Photoelectron Spectroscopy; D010973:Platelet Adhesiveness / Q000187:drug effects; D011817:Rabbits; D013193:Stainless Steel / Q000737:chemistry; D015607:Stents; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D017155:Wettability; D014961:X-Ray Diffraction
D020099:Coated Materials, Biocompatible; D013193:Stainless Steel; D003300:Copper; D014025:Titanium
Copper-titanium film; Endothelialization; Hemocompatibility; Stent