Angiotensin I-converting enzyme (ACE) inhibitory activity of Fucus spiralis macroalgae and influence of the extracts storage temperature-A short report.
Recently, increasing attention has been paid to the marine algae as a natural source of novel angiotensin-I converting enzyme (ACE) inhibitors, such as the phlorotannins that are the predominant polyphenols in brown algae. This study reports, for the first time, the ACE inhibition of methanol extract/fractions from Azorean brown algae Fucus spiralis (Fs) determined by HPLC-UV method, their total phenolic content (TPC) quantified as phloroglucinol equivalents (PE) and the effect of the Fs dry powder methanol extracts (Fs-DME) storage temperature on ACE inhibition. The results indicate that the ACE inhibition of Fs-DME decreased by 28.8% and 78.2% when stored during 15days at -80°C and -13°C, respectively, as compared with the activity of Fs-DME at a refrigerated temperature of 6°C and assayed immediately after extraction that showed a value of 80.1±2.1%. This Fs-DME sample was fractionated by ultrafiltration membranes into three molecular weight ranges (<1kDa, 1-3kDa and >3kDa), presenting the fraction>3kDa remarkably high ACE inhibition (88.8±2.4%), TPC value (156.6±1.4mg PE/g of dry weight fraction) and yield. Furthermore, chromatographic and spectrophotometric analyses corroborate that phenolic compounds were present in Fs methanol extract/fractions, and also revealed that phloroglucinol occurs in Fs. The results seem to suggest that Azorean Fs can be a source of powerful ACE-inhibitory phlorotannins with potential impact on public health, particularly on hypertensive patients.
CITA-A-Research Center for Agricultural Technology, University of Azores, 9501-801 Ponta Delgada, S. Miguel, Azores, Portugal.
Lisete
L
Paiva
CITA-A-Research Center for Agricultural Technology, University of Azores, 9501-801 Ponta Delgada, S. Miguel, Azores, Portugal. Electronic address: elis@uac.pt.
Elisabete
E
Lima
cE3c-Centre for Ecology, Evolution and Environmental Changes/Azorean Biodiversity Group, and University of Azores, Department of Biology, 9501-801 Ponta Delgada, S. Miguel, Azores, Portugal.
Ana Isabel
AI
Neto
CITA-A-Research Center for Agricultural Technology, University of Azores, 9501-801 Ponta Delgada, S. Miguel, Azores, Portugal.
José
J
Baptista
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D004356:Drug Storage / Q000379:methods; D044645:Fucus; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D012624:Seaweed; D013696:Temperature
D000806:Angiotensin-Converting Enzyme Inhibitors; D007703:Peptidyl-Dipeptidase A
ACE-inhibitory activity; HPLC; Hypertension; Phlorotannins; Sample stability studies; Total phenolic content