Differentially expressed genes after viral haemorrhagic septicaemia virus infection in olive flounder (Paralichthys olivaceus).
A strain of viral haemorrhagic septicaemia virus (VHSV) was isolated from cultured olive flounder (Paralichthys olivaceus) during epizootics in South Korean. This strain showed high mortality to olive flounder in in vivo challenge experiment. The complete genomic RNA sequences were determined and phylogenetic analysis of the amino acid sequences of glycoprotein revealed that this isolate was grouped into genotype IVa of genus Novirhabdovirus. Expression profile of genes in olive flounder was analyzed at day 1 and day3 after infection with this VHSV isolate by using cDNA microarray containing olive flounder 13K cDNA clones. Microarray analysis revealed 785 up-regulated genes and 641 down-regulated genes by at least two-fold in virus-infected fish compared to healthy control groups. Among 785 up-regulated genes, we identified seven immune response-associated genes, including the interferon (IFN)-induced 56-kDa protein (IFI56), suppressor of cytokine signaling 1 (SOCS1), interleukin 8 (IL-8), cluster of differentiation 83 (CD83), α-globin (HBA), VHSV-induced protein-6 (VHSV6), and cluster of differentiation antigen 9 (CD9). Our results confirm previous reports that even virulent strain of VHSV induces expression of genes involved in protective immunity against VHSV.
Pathology Division, National Institute of Fisheries Science (NIFS), 216 GijangHaean-Ro, Gijang-up, Gijang-Gun, Busan 46083, Republic of Korea.
Jee Youn
JY
Hwang
Pathology Division, National Institute of Fisheries Science (NIFS), 216 GijangHaean-Ro, Gijang-up, Gijang-Gun, Busan 46083, Republic of Korea.
Mun-Gyeong
MG
Kwon
Pathology Division, National Institute of Fisheries Science (NIFS), 216 GijangHaean-Ro, Gijang-up, Gijang-Gun, Busan 46083, Republic of Korea.
Jung Soo
JS
Seo
Pathology Division, National Institute of Fisheries Science (NIFS), 216 GijangHaean-Ro, Gijang-up, Gijang-Gun, Busan 46083, Republic of Korea.
Jung Wan
JW
Do
Pathology Division, National Institute of Fisheries Science (NIFS), 216 GijangHaean-Ro, Gijang-up, Gijang-Gun, Busan 46083, Republic of Korea.
Myoung-Ae
MA
Park
Pathology Division, National Institute of Fisheries Science (NIFS), 216 GijangHaean-Ro, Gijang-up, Gijang-Gun, Busan 46083, Republic of Korea.
Sung-Hee
SH
Jung
Pathology Division, National Institute of Fisheries Science (NIFS), 216 GijangHaean-Ro, Gijang-up, Gijang-Gun, Busan 46083, Republic of Korea. Electronic address: sjahn@kimst.re.kr.
Sang Jung
SJ
Ahn
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D005393:Fish Diseases / Q000276:immunology* / Q000401:mortality / Q000821:virology; D005432:Flounder / Q000276:immunology* / Q000821:virology; D020869:Gene Expression Profiling / Q000662:veterinary; D016679:Genome, Viral / Q000235:genetics*; D031941:Hemorrhagic Septicemia, Viral / Q000235:genetics / Q000276:immunology* / Q000401:mortality / Q000821:virology; D054884:Host-Pathogen Interactions; D007136:Immunoglobulins / Q000378:metabolism; D007372:Interferons / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D029141:Novirhabdovirus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D000071222:Suppressor of Cytokine Signaling 1 Protein / Q000378:metabolism; D060245:Tetraspanin 29 / Q000378:metabolism; D014774:Virulence; D000097802:CD83 Antigen
D015703:Antigens, CD; D007136:Immunoglobulins; D007372:Interferons; D016209:Interleukin-8; D008562:Membrane Glycoproteins; D000071222:Suppressor of Cytokine Signaling 1 Protein; D060245:Tetraspanin 29; D000097802:CD83 Antigen
Glycoprotein; Olive flounder; Paralichthys olivaceus; Rt-pcr; Viral haemorrhagic septicaemia virus; cDNA microarray