CTXϕ: Exploring new alternatives in host factor-mediated filamentous phage replications.
For a long time Ff phages from Escherichia coli provided the majority of the knowledge about the rolling circle replication mechanism of filamentous phages. Host factors involved in coliphages replication have been fully identified. Based on these studies, the function of Rep protein as the accessory helicase directly implicated in filamentous phage replication was considered a paradigm. We recently reported that the replication of some filamentous phages from Vibrio cholerae, including the cholera toxin phage CTXϕ, depended on the accessory helicase UvrD instead of Rep. We also identified HU protein as one of the host factors involved in CTXϕ and VGJϕ replication. The requirement of UvrD and HU for rolling circle replication was previously reported in some family of plasmids but had no precedent in filamentous phages. Here, we enrich the discussion of our results and present new preliminary data highlighting remarkable divergence in the lifestyle of filamentous phages.
Southern Research Institute, Department of Biochemistry and Molecular Biology, Drug Discovery Division, Birmingham, AL, USA; Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Univ. Paris Sud, Gif sur Yvette, France.
Eriel
E
Martínez
Southern Research Institute, Department of Biochemistry and Molecular Biology, Drug Discovery Division , Birmingham, AL, USA.
Javier
J
Campos-Gómez
Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Univ. Paris Sud , Gif sur Yvette, France.
François-Xavier
FX
Barre
Cholera; Xer recombination; filamentous phage; integrative mobile element; rolling circle replication