Autologous fat injection therapy including a high concentration of adipose-derived regenerative cells in a vocal fold paralysis model: animal pilot study.
OBJECTIVE
To verify the effectiveness and safety of the addition of adipose-derived regenerative cells to autologous fat injection therapy.


METHODS
Unilateral vocal fold paralysis models were made by cutting the right recurrent laryngeal nerve in two pigs. At day 30, 0.5 ml adipose-derived regenerative cells mixed with 1 ml autologous fat was injected into the right vocal fold of one pig, with the other receiving 0.5 ml Ringer's solution mixed with 1 ml autologous fat. At day 120, fibrescopy, laser Doppler flowmeter, computed tomography, vocal function evaluation and histological assessment were conducted.


RESULTS
Although histological assessment revealed atrophy of the thyroarytenoid muscle fibre in both pigs, there was remarkable hypertrophy of the thyroarytenoid muscle fibre in the area surrounding the adipose-derived regenerative cells injection site.


CONCLUSIONS
The addition of a high concentration of adipose-derived regenerative cells to autologous fat injection therapy has the potential to improve the treatment outcome for unilateral vocal fold paralysis.
Department of Otorhinolaryngology,Nagoya University Graduate School of Medicine,Aichi,Japan.
N
N
Nishio
Department of Otorhinolaryngology,Nagoya University Graduate School of Medicine,Aichi,Japan.
Y
Y
Fujimoto
Department of Otorhinolaryngology,Nagoya University Graduate School of Medicine,Aichi,Japan.
K
K
Suga
Department of Otorhinolaryngology,Fujita Health University,Aichi,Japan.
Y
Y
Iwata
Department of Plastic and Reconstructive Surgery,Nagoya University Graduate School of Medicine,Aichi,Japan.
K
K
Toriyama
Department of Plastic and Reconstructive Surgery,Nagoya University Graduate School of Medicine,Aichi,Japan.
K
K
Takanari
Department of Plastic and Reconstructive Surgery,Nagoya University Graduate School of Medicine,Aichi,Japan.
Y
Y
Kamei
Department of Urology,Nagoya University Graduate School of Medicine,Aichi,Japan.
T
T
Yamamoto
Department of Urology,Nagoya University Graduate School of Medicine,Aichi,Japan.
M
M
Gotoh
D017667:Adipocytes / Q000166:cytology / Q000637:transplantation*; D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D004195:Disease Models, Animal; D006984:Hypertrophy / Q000209:etiology; D007821:Laryngeal Muscles / Q000473:pathology; D007830:Larynx / Q000473:pathology; D009133:Muscular Atrophy / Q000209:etiology; D010865:Pilot Projects; D033581:Stem Cell Transplantation / Q000379:methods*; D013552:Swine; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014826:Vocal Cord Paralysis / Q000628:therapy*; D014827:Vocal Cords / Q000473:pathology
Animal; Hoarseness; Injections; Stem Cells; Vocal Cord Paralysis