Process Optimization on Micro-Aeration Supply for High Production Yield of 2,3-Butanediol from Maltodextrin by Metabolically-Engineered Klebsiella oxytoca.
An optimization process with a cheap and abundant substrate is considered one of the factors affecting the price of the production of economical 2,3-Butanediol (2,3-BD). A combination of the conventional method and response surface methodology (RSM) was applied in this study. The optimized levels of pH, aeration rate, agitation speed, and substrate concentration (maltodextrin) were investigated to determine the cost-effectiveness of fermentative 2,3-BD production by metabolically-engineered Klebsiella oxytoca KMS005. Results revealed that pH, aeration rate, agitation speed, and maltodextrin concentration at levels of 6.0, 0.8 vvm, 400 rpm, and 150 g/L respectively were the optimal conditions. RSM also indicated that the agitation speed was the most influential parameter when either agitation and aeration interaction or agitation and substrate concentration interaction played important roles for 2,3-BD production by the strain from maltodextrin. Under interim fed-batch fermentation, 2,3-BD concentration, yield, and productivity were obtained at 88.1±0.2 g/L, 0.412±0.001 g/g, and 1.13±0.01 g/L/h respectively within 78 h.
Metabolic Engineering Research Unit, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Ave., Suranaree Sub-district, Muang District, Nakhon Ratchasima, 30000, Thailand.
Sitha
S
Chan
Division of Biopharmacy, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Warinchamrap, Ubon Ratchathani, 34190, Thailand.
Sirima Suvarnakuta
SS
Jantama
Metabolic Engineering Research Unit, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Ave., Suranaree Sub-district, Muang District, Nakhon Ratchasima, 30000, Thailand.
Sunthorn
S
Kanchanatawee
Metabolic Engineering Research Unit, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Ave., Suranaree Sub-district, Muang District, Nakhon Ratchasima, 30000, Thailand.
Kaemwich
K
Jantama
D019149:Bioreactors; D002072:Butylene Glycols / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D041121:Klebsiella oxytoca / Q000235:genetics / Q000378:metabolism*; D060847:Metabolic Engineering; D011134:Polysaccharides / Q000737:chemistry
D002072:Butylene Glycols; D011134:Polysaccharides; C026978:2,3-butylene glycol; C008315:maltodextrin