Gold nanoparticle-capped mesoporous silica-based H2O2-responsive controlled release system for Alzheimer's disease treatment.
Metal ions promote Alzheimer's disease (AD) pathogenesis by accelerating amyloid-β (Aβ) aggregation and inducing formation of neurotoxic reactive oxygen species (ROS) such as hydrogen peroxide (H2O2). Although metal chelators can block these effects, their therapeutic potential is marred by their inability to cross the blood-brain barrier (BBB) and by their non-specific interactions with metal ions necessary for normal cellular processes, which could result in adverse side effects. To overcome these limitations, we created a novel gold nanoparticle-capped mesoporous silica (MSN-AuNPs) based H2O2-responsive controlled release system for targeted delivery of the metal chelator CQ. In this system, CQ is released only upon exposure to conditions in which H2O2levels are high, such as those in Aβ plaques. The conjugation of AuNPs on the surface of MSN did not affect their ability to cross the BBB. The AuNPs also help in decrease the Aβ self-assembly, due to this, MSN-CQ-AuNPs were more efficient than MSN-CQ in inhibiting Cu2+-induced Aβ40aggregation. Furthermore, MSN-CQ-AuNPs reduced the cell membrane disruption, microtubular defects and ROS-mediated apoptosis induced by Aβ40-Cu2+complexes. The high BBB permeability, efficient anti-Aβ aggregation, and good biocompatibility of MSN-CQ-AuNPs, together with the specific conditions necessary for its release of CQ, demonstrate its potential for future biomedical applications.



Due to the low ability to cross the blood-brain barrier (BBB) and non-specific interactions with metal ions necessary for normal cellular processes of metal chelator or Aβ inhibitors, we created a novel gold nanoparticle-capped mesoporous silica (MSN-AuNPs)-based H2O2-responsive controlled release system for targeted delivery of the metal chelator CQ and AuNPs (Aβ inhibitor). In this system, CQ and AuNPs are released only upon exposure to conditions in which H2O2levels are high, such as those in Aβ plaques. The AuNPs on the surface of MSN also help in decrease the Aβ self-assembly, due to this, MSN-CQ-AuNPs were more efficient than MSN-CQ in inhibiting Cu2+-induced Aβ40aggregation. Furthermore, MSN-CQ-AuNPs reduced the cell membrane disruption, microtubular defects and ROS-mediated apoptosis induced by Aβ40-Cu2+complexes. Our data suggest that this controlled release system may have widespread application in the field of medicine for Alzheimer's disease.
Department of Chemistry, Jinan University, Guangzhou 510632, PR China.
Licong
L
Yang
Department of Chemistry, Jinan University, Guangzhou 510632, PR China.
Tiantian
T
Yin
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong.
Yanan
Y
Liu
Department of Chemistry, Jinan University, Guangzhou 510632, PR China.
Jing
J
Sun
Department of Chemistry, Jinan University, Guangzhou 510632, PR China.
Yanhui
Y
Zhou
Department of Chemistry, Jinan University, Guangzhou 510632, PR China. Electronic address: tliuliu@jnu.edu.cn.
Jie
J
Liu
D000544:Alzheimer Disease / Q000188:drug therapy*; D016229:Amyloid beta-Peptides / Q000378:metabolism / Q000633:toxicity; D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D048430:Cell Shape / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D003692:Delayed-Action Preparations; D065546:Drug Liberation; D005455:Fluorescent Antibody Technique; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D009498:Neurotoxins / Q000633:toxicity; D016716:PC12 Cells; D016062:Porosity; D051381:Rats; D012235:Rhodamines / Q000032:analysis; D012822:Silicon Dioxide / Q000737:chemistry; D014404:Tubulin / Q000378:metabolism; D014961:X-Ray Diffraction
D016229:Amyloid beta-Peptides; D002614:Chelating Agents; D003692:Delayed-Action Preparations; D009498:Neurotoxins; D012235:Rhodamines; D014404:Tubulin; D006046:Gold; D012822:Silicon Dioxide; D003300:Copper; D006861:Hydrogen Peroxide; C029773:rhodamine B
Alzheimer’s disease; Amyloid-β; Controlled release system; Mesoporous silica nanoparticle; Metal ions