Improved Human Bone Marrow Mesenchymal Stem Cell Osteogenesis in 3D Bioprinted Tissue Scaffolds with Low Intensity Pulsed Ultrasound Stimulation.
3D printing and ultrasound techniques are showing great promise in the evolution of human musculoskeletal tissue repair and regeneration medicine. The uniqueness of the present study was to combine low intensity pulsed ultrasound (LIPUS) and advanced 3D printing techniques to synergistically improve growth and osteogenic differentiation of human mesenchymal stem cells (MSC). Specifically, polyethylene glycol diacrylate bioinks containing cell adhesive Arginine-Glycine-Aspartic acid-Serene (RGDS) peptide and/or nanocrystalline hydroxyapatite (nHA) were used to fabricate 3D scaffolds with different geometric patterns via novel table-top stereolithography 3D printer. The resultant scaffolds provide a highly porous and interconnected 3D environment to support cell proliferation. Scaffolds with small square pores were determined to be the optimal geometric pattern for MSC attachment and growth. The optimal LIPUS working parameters were determined to be 1.5 MHz, 20% duty cycle with 150 mW/cm(2) intensity. Results demonstrated that RGDS peptide and nHA containing 3D printed scaffolds under LIPUS treatment can greatly promote MSC proliferation, alkaline phosphatase activity, calcium deposition and total protein content. These results illustrate the effectiveness of the combination of LIPUS and biomimetic 3D printing scaffolds as a valuable combinatorial tool for improved MSC function, thus make them promising for future clinical and various regenerative medicine application.
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington DC 20052, USA.
Xuan
X
Zhou
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington DC 20052, USA.
Nathan J
NJ
Castro
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington DC 20052, USA.
Wei
W
Zhu
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington DC 20052, USA.
Haitao
H
Cui
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington DC 20052, USA.
Mitra
M
Aliabouzar
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington DC 20052, USA.
Kausik
K
Sarkar
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington DC 20052, USA.|Department of Biomedical Engineering, The George Washington University, Washington DC 20052, USA.|Department of Medicine, The George Washington University, Washington DC 20052, USA.
Lijie Grace
LG
Zhang
D001854:Bone Marrow Cells / Q000166:cytology* / Q000528:radiation effects; D001861:Bone Regeneration / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects*; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000528:radiation effects; D010012:Osteogenesis / Q000502:physiology*; D066330:Printing, Three-Dimensional; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D000069453:Ultrasonic Waves