miR-582-5P induces colorectal cancer cell proliferation by targeting adenomatous polyposis coli.
BACKGROUND
microRNA (miRNAs) dysregulation is widely involved in cancer progression and contributed to sustained cell proliferation by directly targeting multiple targets. Therefore, better understand the underlying mechanism of miRNA in carcinogenesis may improve diagnostic and therapeutic strategies for malignancy.


METHODS
We assessed microRNA-582 (miR-582-5P) expression in colorectal cancer (CRC) specimens and cell lines by real-time PCR. Luciferase reporter assay was used to confirm the target associations. Colony formation assay and anchorage-independent growth assay were used to analyze the effect of miR-582-5P on cell proliferation. Adenomatous polyposis coli (APC) gene and protein expression were examined using real-time PCR and western blotting, respectively.


RESULTS
miR-582-5P was upregulated in the CRC specimens and cell lines and targeted the 3' untranslated region of APC directly. miR-582-5P overexpression increased cyclin D1 and c-MYC expression, which subsequently induced CRC cell proliferation in an APC-dependent manner.


CONCLUSIONS
Our findings suggest that miR-582-5P plays an important role in the progression of CRC by inducing proliferation and may identify new targets for anti-cancer treatment.
Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital, Jilin University, ChangChun, 130033, China.
Zhenbo
Z
Shu
Department of Ultrasound, China-Japan Union Hospital, Jilin University, ChangChun, 130033, China.
Libo
L
Chen
Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital, Jilin University, ChangChun, 130033, China. zwh_xkw2014@163.com.
Dayong
D
Ding
D020413:3' Untranslated Regions; D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics*; D015153:Blotting, Western; D063646:Carcinogenesis / Q000235:genetics*; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D015972:Gene Expression Regulation, Neoplastic; D019073:HT29 Cells; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
D020413:3' Untranslated Regions; C555337:APC protein, human; D025601:Adenomatous Polyposis Coli Protein; C000593706:MIRN582 microRNA, human; D035683:MicroRNAs
APC; Colorectal cancer; Proliferation; miR-582-5P