[Microbiological analysis of red octopus in fishing ports of Campeche, Mexico].
OBJECTIVE
In this work we studied the microbiological quality of the red octopus given its important economic and social impact on the region South-Southeast of Mexico.


METHODS
Samples were taken in different areas of capture of the species and analyzed with biochemical tests described in the Mexican official standards, identifying strains belonging to the genus Vibrio, Salmonella and faecal coliforms, and E. coli O157: H7. We used the BAx System for the identification of microorganisms through their bacterial DNA. The results obtained in biochemical and molecular methods were confirmed.


RESULTS
Bland-Altman statistical method pointed out that both techniques can be used interchangeably. McNemar test showed that both methods have the same efficacy for the identification of pathogens (value X2=0.5 ρ=0.4795).


CONCLUSIONS
The microbiological quality of the octopus in the South-Southeast region of Mexico is deficient due to the presence of pathogenic intestinal flora that might represent an epidemiological risk. The indexes established by the regulations suggest the need to apply effective and rapid identification technologies, such as the BAx System.This alternative method of analysis can contribute to the implementation of effective strategies that allow compliance with the minimal sanitary specifications during the processing of fishing products, thus strengthening the control systems to decrease the risks of epidemiological outbreaks in the region.
Neyi
N
Estrella-Gómez
Diana
D
Escalante-Réndiz
Araceli
A
González-Burgos
Delta
D
Sosa-Cordero
Rafael
R
Rojas-Herrera
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D004755:Enterobacteriaceae / Q000302:isolation & purification; D005516:Food Microbiology; D008800:Mexico; D049831:Octopodiformes / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000302:isolation & purification; D014733:Vibrio / Q000302:isolation & purification
D004269:DNA, Bacterial