Copper chelation by trientine dihydrochloride inhibits liver RFA-induced inflammatory responses in vivo.
OBJECTIVE
Hepatocellular carcinoma (HCC) is the fifth most common cancer and the third most common cause of cancer-related death worldwide. Radiofrequency ablation (RFA) is currently performed widely for managing HCC. RFA treatment causes damage around the ablation. Trientine dihydrochloride has been used to reduce the copper in liver.


METHODS
The rats were treated with trientine dihydrochloride for 5 days before liver RFA. Liver function, copper concentration, inflammation biomarkers and MDA, SOD were analyzed after RFA treatment for 2 h, 2 and 5 days.


RESULTS
The results indicated that trientine dihydrochloride reduced the copper in plasma and liver tissue significantly. And trientine dihydrochloride significantly inhibited RFA-induced inflammatory gene expression in liver. Similar inhibitory effects of trientine dihydrochloride were observed on ROS-induced malondialdehyde production in liver tissues.


CONCLUSIONS
These results suggest that pre-treatment with the selective copper chelator trientine dihydrochloride can inhibit inflammatory response effectively during and after liver RFA in vivo. Chelation of copper to a lower level before liver RFA may be a novel strategy to prevent or ameliorate inflammatory responses in liver induced by RFA and to protect the parenchyma tissues in liver during and after RFA in HCC patients.
Beijing Youan Hospital, Capital Medical University, No. 8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China.|Beijing Institute of Hepatology, No. 8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China.
Ji-Ming
JM
Yin
Beijing Youan Hospital, Capital Medical University, No. 8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China.
Li-Bo
LB
Sun
Beijing Youan Hospital, Capital Medical University, No. 8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China.
Jia-Sheng
JS
Zheng
Beijing Youan Hospital, Capital Medical University, No. 8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China.
Xin-Xin
XX
Wang
Beijing Youan Hospital, Capital Medical University, No. 8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China.|Beijing Institute of Hepatology, No. 8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China.
De-Xi
DX
Chen
Beijing Youan Hospital, Capital Medical University, No. 8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China. liningbjyah@126.com.|Beijing Institute of Hepatology, No. 8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China. liningbjyah@126.com.
Ning
N
Li
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000097:blood; D017115:Catheter Ablation; D002614:Chelating Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003300:Copper / Q000097:blood / Q000378:metabolism*; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D014266:Trientine / Q000494:pharmacology / Q000627:therapeutic use*
D000893:Anti-Inflammatory Agents; D002614:Chelating Agents; D016207:Cytokines; D008315:Malondialdehyde; D003300:Copper; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D014266:Trientine
Hepatocellular carcinoma; Inflammation; Liver radiofrequency ablation; Trientine dihydrochloride