Dengue virus surveillance: Detection of DENV-4 in the city of São José do Rio Preto, SP, Brazil.
Dengue viruses are the most common arbovirus infection worldwide and are caused by four distinct serotypes of the dengue virus (DENV). In the present study, we assessed DENV transmission in São José do Rio Preto (SJRP) from 2010 to 2014. We analyzed blood samples from febrile patients who were attended at health care centers in SJRP. DENV detection was performed using multiplex RT-PCR, using flavivirus generic primers, based on the genes of the non-structural protein (NS5), followed by nested-PCR assay with species-specific primers. We analyzed 1549 samples, of which 1389 were positive for NS1 by rapid test. One thousand and eight-seven samples (78%) were confirmed as positive by multiplex RT-PCR: DENV-4, 48.5% (528/1087); DENV-1, 41.5% (449/1087); DENV-2, 9.5% (104/1087); and co-infection (5 DENV-1/DENV-4, 1 DENV-1/DENV-2), 0.5% (6/1087). Phylogenetic analysis of the DENV-4 grouped the isolates identified in this study with the American genotype and the showed a relationship between isolates from SJRP and isolates from the northern region of South America. Taken together, our data shows the detection and emergence of new dengue genotype in a new region and reiterate the importance of surveillance programs to detect and trace the evolution of DENV.
Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
Tatiana Elias
TE
Colombo
Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
Danila
D
Vedovello
Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
Carolina Colombelli
CC
Pacca-Mazaro
Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
Adriano
A
Mondini
Laboratório de Virologia do Instituto de Biociências de Botucatu - Universidade Estadual Paulista, Botucatu, SP, Brazil.
João Pessoa
JP
Araújo
Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
Eliana
E
Cabrera
Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
José Carlos
JC
Lopes
Prefeitura de São José do Rio Preto, Departamento de Vigilância em Saúde, São José do Rio Preto, SP, Brazil.
Izalco Nuremberg
IN
Penha Dos Santos
Prefeitura de São José do Rio Preto, Departamento de Vigilância em Saúde, São José do Rio Preto, SP, Brazil.
Andréia Francesli
AF
Negri Reis
Prefeitura de São José do Rio Preto, Departamento de Vigilância em Saúde, São José do Rio Preto, SP, Brazil.
Fabiana Rodrigues
FR
Costa
Prefeitura de São José do Rio Preto, Departamento de Vigilância em Saúde, São José do Rio Preto, SP, Brazil.
Lilian Elisa Arão
LEA
Antônio Cruz
Prefeitura de São José do Rio Preto, Departamento de Vigilância em Saúde, São José do Rio Preto, SP, Brazil.
Jurandir
J
Ferreira
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Brazil.
Eliseu Soares
ES
de Oliveira Rocha
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Brazil.
Erna Geessien
EG
Kroon
Instituto de Ciências da Saúde, Universidade Federal do Mato Grosso, Sinop, MT, Brazil.
Roberta Vieira
RV
de Morais Bronzoni
Institute for Human Infections and Immunity, Center for Tropical Diseases, Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
Nikos
N
Vasilakis
Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil. Electronic address: mnogueira@famerp.br.
Maurício Lacerda
ML
Nogueira
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D060085:Coinfection; D003715:Dengue / Q000097:blood / Q000453:epidemiology*; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D010802:Phylogeny; D065288:Serogroup; D014505:Urban Population; D055815:Young Adult
Dengue virus; Phylogenetic analysis; Surveillance programs; Transmission