Intralipid supplementation in women with recurrent spontaneous abortion and elevated levels of natural killer cells.
OBJECTIVE
To investigate the efficacy of intralipid supplementation in women with recurrent spontaneous abortion (RSA) and elevated natural killer cell activity undergoing in vitro fertilization/intracytoplasmic sperm injection.


METHODS
Between February 10, 2013, and April 30, 2015, a double-blind randomized controlled study was conducted at a center in Egypt. Women with unexplained secondary infertility, RSA, and elevated levels of natural killer cells (>12%) were enrolled and randomly assigned to receive intralipid (2mL diluted at 20% in 250mL saline) or saline (250mL) infusion on the day of oocyte retrieval using random numbers and sealed envelopes. Patients and attending physicians were masked to group assignment. The infusions were repeated within 1week of a positive pregnancy test and then every 2weeks until the end of the first trimester. The primary outcome was chemical pregnancy 14days after embryo transfer. Analyses were by intention-to-treat.


RESULTS
Overall, 296 women were enrolled. Chemical pregnancy was recorded for 84 (58.3%) of 144 women in the intralipid group and 76 (50.0%) of 152 in the control group (P=0.129).


CONCLUSIONS
Intralipid supplementation did not increase frequency of chemical pregnancy. However, findings related to ongoing pregnancy and live birth should be investigated further. ClinicalTrials.gov:NCT01788540.
Department of Obstetrics and Gynecology, Cairo University, Cairo, Egypt. Electronic address: dinadakhly@gmail.com.
Dina M R
DM
Dakhly
Department of Obstetrics and Gynecology, Cairo University, Cairo, Egypt.
Yomna A
YA
Bayoumi
Department of Obstetrics and Gynecology, Cairo University, Cairo, Egypt.
Mohamed
M
Sharkawy
Department of Obstetrics and Gynecology, Cairo University, Cairo, Egypt.
Sherine H
SH
Gad Allah
Department of Obstetrics and Gynecology, Cairo University, Cairo, Egypt.
Mohamed A
MA
Hassan
Department of Obstetrics and Gynecology, Cairo University, Cairo, Egypt.
Hisham M
HM
Gouda
Department of Obstetrics and Gynecology, Cairo University, Cairo, Egypt.
Ahmed T
AT
Hashem
Department of Obstetrics and Gynecology, Cairo University, Cairo, Egypt.
Dina L
DL
Hatem
Department of Obstetrics and Gynecology, Cairo University, Cairo, Egypt.
Mona F
MF
Ahmed
Department of Obstetrics and Gynecology, Cairo University, Cairo, Egypt.
Waleed
W
El-Khayat
D000026:Abortion, Habitual / Q000628:therapy*; D000328:Adult; D004311:Double-Blind Method; D004534:Egypt; D004655:Emulsions / Q000008:administration & dosage; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology; D050498:Live Birth; D054315:Oocyte Retrieval / Q000706:statistics & numerical data; D010743:Phospholipids / Q000008:administration & dosage*; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D011261:Pregnancy Trimester, First; D013024:Soybean Oil / Q000008:administration & dosage*; D020554:Sperm Injections, Intracytoplasmic
D004655:Emulsions; D010743:Phospholipids; C545823:soybean oil, phospholipid emulsion; D013024:Soybean Oil
In vitro fertilization; Intracytoplasmic sperm injection; Intralipid; Natural killer cells; Recurrent spontaneous abortion