Targeting Motor End Plates for Delivery of Adenoviruses: An Approach to Maximize Uptake and Transduction of Spinal Cord Motor Neurons.
Gene therapy can take advantage of the skeletal muscles/motor neurons anatomical relationship to restrict gene expression to the spinal cord ventral horn. Furthermore, recombinant adenoviruses are attractive viral-vectors as they permit spatial and temporal modulation of transgene expression. In the literature, however, several inconsistencies exist with regard to the intramuscular delivery parameters of adenoviruses. The present study is an evaluation of the optimal injection sites on skeletal muscle, time course of expression and mice's age for maximum transgene expression in motor neurons. Targeting motor end plates yielded a 2.5-fold increase in the number of transduced motor neurons compared to injections performed away from this region. Peak adenoviral transgene expression in motor neurons was detected after seven days. Further, greater numbers of transduced motor neurons were found in juvenile (3-7 week old) mice as compared with adults (8+ weeks old). Adenoviral injections produced robust transgene expression in motor neurons and skeletal myofibres. In addition, dendrites of transduced motor neurons were shown to extend well into the white matter where the descending motor pathways are located. These results also provide evidence that intramuscular delivery of adenovirus can be a suitable gene therapy approach to treat spinal cord injury.
Translational Neuroscience Facility, School of Medical Sciences, The University of New South Wales, Sydney, NSW, 2052, Australia.
Andrew Paul
AP
Tosolini
Translational Neuroscience Facility, School of Medical Sciences, The University of New South Wales, Sydney, NSW, 2052, Australia.
Renée
R
Morris
D000256:Adenoviridae; D000818:Animals; D015316:Genetic Therapy / Q000379:methods*; D008297:Male; D051379:Mice; D009045:Motor Endplate / Q000378:metabolism* / Q000821:virology; D009046:Motor Neurons / Q000378:metabolism* / Q000821:virology; D013116:Spinal Cord / Q000378:metabolism* / Q000821:virology; D014161:Transduction, Genetic / Q000379:methods*