Permanganate-assisted removal of PCR inhibitors during the DNA Chelex extraction from stained denim samples.
In this study, it was demonstrated that the DNA Chelex extraction combined with the permanganate assisted-oxidation is highly efficient in removing the PCR inhibitors often found in clothing materials, such as phthalocyanine. The extraction assays were conducted in saliva, blood and epithelial cells samples mixed with three oxidation-resistant dye copper(II) α-phthalocyanine, copper(II) β-phthalocyanine and tetrasulfonated copper(II) β-phthalocyanine. After DNA amplification, all samples were able to provide full DNA profiles. The permanganate/Chelex system was tested further on denim-stained samples and displayed the same ability to remove the PCR inhibitors from the commercial textile materials.
National Forensic Science Institute, Şoseaua Ştefan cel Mare 13-15, sect. 2, 020123, Bucharest, Romania.|Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta, Nr. 4-12, Bucharest, 030018, Romania.
Sorina
S
Pîrlea
Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta, Nr. 4-12, Bucharest, 030018, Romania.
Mihaela
M
Puiu
Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta, Nr. 4-12, Bucharest, 030018, Romania. adina.raducan@g.unibuc.ro.
Adina
A
Răducan
Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta, Nr. 4-12, Bucharest, 030018, Romania.
Dumitru
D
Oancea
D001774:Blood Chemical Analysis; D002614:Chelating Agents; D003020:Clothing; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods; D004847:Epithelial Cells / Q000737:chemistry; D006801:Humans; D007211:Indoles; D017895:Manganese Compounds; D009942:Organometallic Compounds; D016877:Oxidants; D010087:Oxides; D016133:Polymerase Chain Reaction; D011137:Polystyrenes; D011145:Polyvinyls; D012463:Saliva / Q000737:chemistry
D002614:Chelating Agents; D007211:Indoles; D017895:Manganese Compounds; D009942:Organometallic Compounds; D016877:Oxidants; D010087:Oxides; D011137:Polystyrenes; D011145:Polyvinyls; C048856:permanganic acid; C028418:tetrasulfophthalocyanine; C015445:copper phthalocyanine; C006960:chelex; D004247:DNA
Chelex; DNA amplification; Phthalocyanine; Polymerase chain reaction