NAPPA as a Real New Method for Protein Microarray Generation.
Nucleic Acid Programmable Protein Arrays (NAPPA) have emerged as a powerful and innovative technology for the screening of biomarkers and the study of protein-protein interactions, among others possible applications. The principal advantages are the high specificity and sensitivity that this platform offers. Moreover, compared to conventional protein microarrays, NAPPA technology avoids the necessity of protein purification, which is expensive and time-consuming, by substituting expression in situ with an in vitro transcription/translation kit. In summary, NAPPA arrays have been broadly employed in different studies improving knowledge about diseases and responses to treatments. Here, we review the principal advances and applications performed using this platform during the last years.
Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. pauladg@usal.es.|Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. pauladg@usal.es.
Paula
P
Díez
Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. mariagg@usal.es.|Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. mariagg@usal.es.
María
M
González-González
Rheumatology Division, ProteoRed/ISCIII Proteomics Group, INIBIC, Hospital Universitario de A Coruña, A Coruña 15006, Spain. llourido@udc.es.
Lucía
L
Lourido
Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. romade@usal.es.
Rosa M
RM
Dégano
Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. nibarrola@usal.es.
Nieves
N
Ibarrola
Biotechnology National Centre, Spanish National Research Council (CSIC), Madrid 28049, Spain. jcasado@cnb.csic.es.
Juan
J
Casado-Vela
Biodesign Institute, Arizona State University, 1001 South McAllister Avenue, Tempe, AZ 85287, USA. Joshua.Labaer@asu.edu.
Joshua
J
LaBaer
Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. mfuentes@usal.es.|Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain. mfuentes@usal.es.
Manuel
M
Fuentes
NAPPA; biomarker; high-throughput screening; microarray generation; protein microarray; protein-protein interaction