Genotyping of Staphylococcus aureus in bovine mastitis and correlation to phenotypic characteristics.
Reducing the prevalence of mastitis caused by Staphylococcus aureus (S. aureus) is essential to improve animal health and reduce economic losses for farmers. The clinical outcome of acute mastitis and risk of progression to persistent mastitis can, at least to some extent, be related to genetic variants of the strain causing the infection. In the present study we have used microarrays to investigate the presence of virulence genes in S. aureus isolates from dairy cows with acute clinical mastitis (n=70) and correlated the findings to other genotypic and phenotypic characteristics. Among the most commonly found virulence factors were genes encoding several hemolysin types, leukocidins D and lukM/lukF-P83, clumping factors A and B, fibrinogen binding protein and fibronectin-binding protein A. Some virulence factors e.g. fibronectin-binding protein B and Staphylococcus aureus surface protein G were less common. Genes coding for several staphylococcal enterotoxins and toxic shock syndrome toxin-1 (TSST-1) were commonly found, especially in one major pulsotype. No beta-lactamase genes were found in any common pulsotype, while present in some rare pulsotypes, indicated to be of human origin. Production of TSST-1, enterotoxins, hemolysins and beta-lactamase could all be positively correlated to presence of the corresponding genes. This study reveals a number of genotypic differences and similarities among common and rare pulsotypes of S. aureus from cases of mastitis in Sweden. The results could help the design of diagnostic tools to guide on-farm interventions according to the expected impact on udder health from a specific S. aureus genotype.
National Veterinary Institute (SVA), Uppsala, Sweden. Electronic address: karin.artursson@sva.se.
Karin
K
Artursson
National Veterinary Institute (SVA), Uppsala, Sweden.
Robert
R
Söderlund
National Veterinary Institute (SVA), Uppsala, Sweden.
Lihong
L
Liu
Alere Technologies, Jena, Germany; Institute for Medical Microbiology and Hygiene, TU Dresden, Dresden, Germany.
Stefan
S
Monecke
Applied Microbiology, Faculty of Engineering, Lund University, Lund, Sweden.
Jenny
J
Schelin
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D002417:Cattle; D003612:Dairying; D004768:Enterotoxins / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006460:Hemolysin Proteins / Q000235:genetics*; D007956:Leukocidins / Q000235:genetics; D008321:Mammary Glands, Animal / Q000382:microbiology; D008414:Mastitis, Bovine / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D010641:Phenotype; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D018089:Superantigens / Q000235:genetics*; D013548:Sweden / Q000453:epidemiology; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*
D001427:Bacterial Toxins; D004768:Enterotoxins; D006460:Hemolysin Proteins; D007956:Leukocidins; D018089:Superantigens; D037521:Virulence Factors; C030107:enterotoxin F, Staphylococcal
Genotyping; Mastitis; Microarray; Staphylococcus aureus; Virulence gene