Tetranortriterpenes and Limonoids from the Roots of Aphanamixis polystachya.
Phytochemical investigation of the acetone extract from the roots of Aphanamixis polystachya resulted in isolation of four new tetranortriterpenes (1-4) in addition to one protolimonoid (methyl-1ξ,7R-diacetoxy-23R,25-dihydroxy-20S,24R-21,24-epoxy-3,4-seco-apotirucall-4(28),14(15)-diene-3-oate (5)), five known limonoids (rohituka 3 (6), rohituka 7 (7), nymania 1 (8), rubrin G (9), prieurianin (10)) and a steroid (2,3-dihydroxy-5-pregnan-16-one (11)). Their structures were determined by spectroscopic analyses, including 2D-NMR (COSY, HMQC, HMBC, and NOESY) and high-resolution electrospray ionization mass spectrometry (HRESIMS). Cytotoxic and anti-inflammatory activities of these compounds were evaluated. Compounds 4 and 5 showed significant inhibition against superoxide generation and elastase release by human neutrophils in response to (formyl-l-methionyl-l-leucyl-l-phenylalanine/cytochalasin B) (FMLP/CB).
School of Pharmacy, College of Medicine, National Taiwan University, No. 33, Linsen S. Rd., Zhongzheng Dist., Taipei 10050, Taiwan. r00423014@ntu.edu.tw.
Ching-Jie
CJ
Lin
School of Pharmacy, College of Medicine, National Taiwan University, No. 33, Linsen S. Rd., Zhongzheng Dist., Taipei 10050, Taiwan. d96423006@ntu.edu.tw.
I-Wen
IW
Lo
Department of Life Sciences, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan. z10108042@email.ncku.edu.tw.
Yu-Chi
YC
Lin
Taiwan Forestry Research Institute, Council of Agriculture, Executive Yuan, No. 53, Nanhai Rd., Zhongzheng Dist., Taipei 10066, Taiwan. sychen@tfri.gov.tw.
Shun-Ying
SY
Chen
Taiwan Forestry Research Institute, Council of Agriculture, Executive Yuan, No. 53, Nanhai Rd., Zhongzheng Dist., Taipei 10066, Taiwan. chien@tfri.gov.tw.
Ching-Te
CT
Chien
National Research Institute of Chinese Medicine, Ministry of Health and Welfare, No. 155-1, Sec. 2, Linong St., Beitou District, Taipei 11221, Taiwan. kuoyh@nricm.edu.tw.
Yao-Haur
YH
Kuo
Graduate Institute of Natural Products, School of Traditional Medicine, College of Medicine, and Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan 33302, Taiwan. htl@mail.cgu.edu.tw.|Department of Cosmetic Science and Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33302, Taiwan. htl@mail.cgu.edu.tw.
Tsong-Long
TL
Hwang
Department of Pharmacy, Tajen University, No. 20, Weixin Rd., Yanpu Township, Pingtung 90741, Taiwan. ssliou@tajen.edu.tw.
Shorong-Shii
SS
Liou
School of Pharmacy, College of Medicine, National Taiwan University, No. 33, Linsen S. Rd., Zhongzheng Dist., Taipei 10050, Taiwan. ycshen@ntu.edu.tw.
Ya-Ching
YC
Shen
D006801:Humans; D019272:Leukocyte Elastase / Q000378:metabolism*; D036701:Limonins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D029582:Meliaceae / Q000737:chemistry*; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D013481:Superoxides / Q000378:metabolism*
D036701:Limonins; D010936:Plant Extracts; D013481:Superoxides; D019272:Leukocyte Elastase
Aphanamixis polystachya; anti-inflammatory activity; apotirucallane-type triterpenoid; cytotoxicity; limonoid