Customized sequential multiplex PCR for accurate and early determination of invasive pneumococcal serotypes found in India.
For accurate and earlier detection of invasive pneumococcal serogroup/serotypes from India, we have rearranged the African sequence of multiplex PCR provided by the Centers for Disease Control and Prevention, USA. This modified approach can successfully be adapted for earlier serotype detection of 95% of the pneumococcal strains prevalent in India.
Department of Clinical Microbiology, Christian Medical College and Hospital, Vellore, India. Electronic address: vbalaji@cmcvellore.ac.in.
Balaji
B
Veeraraghavan
Department of Clinical Microbiology, Christian Medical College and Hospital, Vellore, India.
Ranjith
R
Jayaraman
Department of Clinical Microbiology, Christian Medical College and Hospital, Vellore, India.
James
J
John
Department of Clinical Microbiology, Christian Medical College and Hospital, Vellore, India.
Rosemol
R
Varghese
Department of Clinical Microbiology, Christian Medical College and Hospital, Vellore, India.
Ayyanraj
A
Neeravi
Department of Child Health, Christian Medical College and Hospital, Vellore, India.
Valsan Philip
VP
Verghese
Department of General Medicine, Christian Medical College and Hospital, Vellore, India.
Kurien
K
Thomas
D000372:Agglutination Tests / Q000379:methods; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D042241:Early Diagnosis; D006801:Humans; D007194:India; D058865:Introduced Species; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011008:Pneumococcal Infections / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000382:microbiology*; D015995:Prevalence; D065288:Serogroup; D012703:Serotyping / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity
D001426:Bacterial Proteins; C500439:CpsA protein, Streptococcus pneumoniae; D004269:DNA, Bacterial
Customized sequential multiplex PCR; Indian serotypes; Invasive pneumococcal disease; PCV; Pneumococcal serotyping