Valve Tissue Engineering with Living Absorbable Threads.
Tissue engineering (TE) depends on the population of scaffolds with appropriate cells, arranged in a specific physiological direction using a variety of techniques. Here, a novel technique of creating "living threads" is described based on thin (poly(ε-caprolactone) fibers of different diameters (23-243 μm). The fibers readily attract human mesenchymal stem cells (MSCs), which are firmly adhered. These versatile fibers can be used to produce dimensional shapes identical in shape to the cup-like structure of a normal human valve, while preserving the specific orientation of both the cells and the fibers. The MSCs on leaflets and the cells cultured in flask shown similar epitopes expression when analyzed by fluorescence activated cell sorting. Together, these characteristics have important functional implications as living absorbable fibers can be a valuable resource in TE of living tissues, including heart valves.
Sidra Medical and Research Center, P. O. Box 26999, Doha, Qatar.
Albert Ryszard
AR
Liberski
Sidra Medical and Research Center, P. O. Box 26999, Doha, Qatar.
Christophe Michel
CM
Raynaud
Mechanical Engineering and Material Science Department, Military Institute of Engineering (IME), Rio de Janeiro, RJ, 22291-270, Brazil.
Nadia
N
Ayad
Department of Material and Commodity Sciences and Textile Metrology, Lodz University of Technology, ul. Zeromskiego 116, 90-924, Lodz, Poland.
Dorota
D
Wojciechowska
Sidra Medical and Research Center, P. O. Box 26999, Doha, Qatar.
Abbirami
A
Sathappan
D001672:Biocompatible Materials; D002469:Cell Separation; D006351:Heart Valves; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011091:Polyesters; D023822:Tissue Engineering
D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone
heart valve; leaflets; living threads; organ weaving; tissue engineering