A Novel Biopsy Method for Isolating Neural Stem Cells from the Subventricular Zone of the Adult Rat Brain for Autologous Transplantation in CNS Injuries.
Despite all attempts the problem of regeneration in damaged central nervous system (CNS) has remained challenging due to its cellular complexity and highly organized and sophisticated connections. In this regard, stem cell therapy might serve as a viable therapeutic approach aiming either to support the damaged tissue and hence to reduce the subsequent neurological dysfunctions and impairments or to replace the lost cells and re-establish damaged circuitries. Adult neural stem/progenitor cells (NS/PCs) are one of the outstanding cell sources that can be isolated from the subventricular zone (SVZ) of the lateral ventricles. These cells can differentiate into neurons, astrocytes, and oligodendrocytes. Implanting autologous NS/PCs will greatly benefit the patients by avoiding immune rejection after implantation, better survival, and integration with the host tissue. Developing safe and efficient methods in small animal models will provide us with the opportunity to optimize procedures required to achieve successful human autologous NS/PC transplantation in near future. In this chapter, a highly controlled and safe biopsy method for harvesting stem cell containing tissue from the SVZ of adult rat brain is introduced. Then, isolation and expansion of NS/PCs from harvested specimen as well as the techniques to verify proliferation and differentiation capacity of the resulting NS/PCs are discussed. Finally, a method for assessing the biopsy lesion volume in the brain is described. This safe biopsy method in rat provides a unique tool to study autologous NS/PC transplantation in different CNS injury models.
Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.|Shefa Neuroscience Research Center, Khatam-al-Anbia Hospital, Tehran, Iran.
Hadi
H
Aligholi
Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Gholamreza
G
Hassanzadeh
Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Robert-Koch-Strasse 27a, 48149, Münster, Germany.|Department of Neurosurgery and Neurology, Westfälische Wilhelms-Universität Münster, Robert-Koch-Strasse 27a, 48149, Münster, Germany.
Ali
A
Gorji
Neural Stem Cell and Regenerative Neuroscience Laboratory, Department of Anatomical Sciences, Shiraz School of Medicine, Shiraz University of Medical Sciences, 7134853185, Shiraz, Iran. azarihasan@sums.ac.ir.|Shiraz University of Medical Sciences, Shiraz, Iran. azarihasan@sums.ac.ir.
Hassan
H
Azari
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D001706:Biopsy; D001930:Brain Injuries / Q000209:etiology / Q000473:pathology / Q000628:therapy; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004195:Disease Models, Animal; D020547:Lateral Ventricles / Q000166:cytology* / Q000378:metabolism; D008297:Male; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D051381:Rats; D013119:Spinal Cord Injuries / Q000209:etiology / Q000473:pathology / Q000628:therapy; D033581:Stem Cell Transplantation / Q000379:methods; D014182:Transplantation, Autologous
Autologous NS/PC transplantation; Biopsy; Neural stem cells; Rat; Subventricular zone