Responses of soluble microbial products and extracellular polymeric substances to the presence of toxic 2,6-dichlorophenol in aerobic granular sludge system.
The objective of this study was to evaluate the responses of soluble microbial products (SMP) and extracellular polymeric substances (EPS) to the presence of toxic 2,6-dichlorophenol (2,6-DCP) in aerobic granular sludge (AGS) system. Batch experiment showed that NH4+-N removal efficiency significantly decreased from 99.6% to 47.2% in the toxic 2,6-DCP of 20 mg/L. Moreover, the inhibition degrees of 2,6-DCP on (SOUR)H, [Formula: see text] and [Formula: see text] were 7.8%, 32.1% and 9.5%, respectively. The main components of SMP, including protein (PN) and polysaccharide (PS) increased from 2.3 ± 0.74 and 16.8 ± 0.12 mg/L to 66.4 ± 0.56 and 18.0 ± 0.19 mg/L in the presence of 2,6-DCP. Three-dimensional excitation-emission matrix (3D-EEM) spectroscopy identified tryptophan PN-like, humic acid-like and fulvic acid-like substances in the control SMP, and their fluorescence intensities increased after exposure to 2,6-DCP. Synchronous fluorescence spectra suggested that the fluorescence quenching between EPS and 2,6-DCP was a static quenching process. The obtained results could provide insightful information on the responses of microbial products to AGS in the presence of toxic chlorophenols.
School of Resources and Environment, University of Jinan, Jinan 250022, PR China.
Kai
K
Li
School of Resources and Environment, University of Jinan, Jinan 250022, PR China.
Dong
D
Wei
School of Resources and Environment, University of Jinan, Jinan 250022, PR China.
Tao
T
Yan
School of Resources and Environment, University of Jinan, Jinan 250022, PR China. Electronic address: dubin61@gmail.com.
Bin
B
Du
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Qin
Q
Wei
D002733:Chlorophenols / Q000494:pharmacology*; D006812:Humic Substances / Q000032:analysis*; D064307:Microbiota / Q000187:drug effects*; D009566:Nitrates / Q000032:analysis*; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*
D002733:Chlorophenols; D006812:Humic Substances; D009566:Nitrates; D011108:Polymers; D011134:Polysaccharides; D012722:Sewage; D009584:Nitrogen; C032729:2,6-dichlorophenol; C006568:ammonium nitrate
2,6 -dichlorophenol (2,6-DCP); Aerobic granular sludge (AGS); Extracellular polymeric substances (EPS); Soluble microbial products (SMP)