Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice.
This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3(GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC). The GLPS3was prepared from cultured mycelium and markedly alleviated the pancreatitis in mice through decreasing lipase, AMS, IFN-γ and TNF-α level as well as increasing SOD and total antioxidant activity. Furthermore, high throughput sequencing analysis revealed that GLPS3altered the composition and diversity of intestinal microbiota, especially, decreased the relative abundance of phylum Bacteroidetes and increased that of phylum Firmictutes. At the genus level, supplementation of GLPS3increased the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae. These results disclosed the potential therapy mechanism of GLPS3on chronic pancreatitis might be intestinal microbiota dependent.
College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, PR China.
Koukou
K
Li
College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, PR China.
Cheng
C
Zhuo
College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, PR China.
Chunying
C
Teng
College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, PR China.
Sumei
S
Yu
College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, PR China.
Xin
X
Wang
College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, PR China.
Yang
Y
Hu
College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, PR China.
Guangming
G
Ren
Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China.
Min
M
Yu
College of Resources and Environmental Science, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: juanjuan4050234@163.com.
Juanjuan
J
Qu
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D041963:Bacteroidetes / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D000068536:Bacillota / Q000235:genetics; D062610:Fungal Polysaccharides / Q000494:pharmacology* / Q000627:therapeutic use; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D008813:Mice, Inbred ICR; D058889:Molecular Typing; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology; D050500:Pancreatitis, Chronic / Q000188:drug therapy*; D020560:Proteobacteria / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020940:Reishi / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D016207:Cytokines; D062610:Fungal Polysaccharides; D012336:RNA, Ribosomal, 16S; D013482:Superoxide Dismutase
Chronic pancreatitis; Ganoderma lucidum polysaccharide; High throughput sequencing; Intestinal microbiota