Ectopic osteogenic capacity of freshly isolated adipose-derived stromal vascular fraction cells supported with platelet-rich plasma: A simulation of intraoperative procedure.
Bone defects represent a serious problem in cranio-maxillofacial surgery. Autologous adipose-derived stromal vascular fraction (SVF) cells in combination with biological factors and bone substitutes were previously proposed as alternative to bone grafting. By simulating an intraoperative procedure we examined osteogenic capacity of the combination of two autologous components, freshly isolated adipose-derived SVF cells, and platelet-rich plasma (PRP), delivered on bone mineral matrix (BMM) carrier (SPB group) in mice ectopic bone forming model. Implantation of BMM only (B group) was a control. The presence of adipose-derived stem cells (ADSCs) in SVF was detected by immunocytochemical analysis. Expression of bone- and endothelial-related genes was compared between freshly isolated SVF and ADSCs obtained from SVF after in vitro cultivation. The implants were analyzed using expression analysis of bone-related genes at one, two, four and eight weeks and histochemical, immunohistochemical and histomorphometrical analyses at two and eight weeks after implantation. Freshly isolated adipose-derived SVF contained ADSCs and exhibited promising osteogenic and vasculogenic capacity. At two and four weeks, significantly higher expression of bone-related genes was detected in SPB group compared to B group. The signs of osteogenic process were more pronounced in SPB than in B implants. By the end of experiment, percentage of infiltrated tissue and vascularization was significantly higher in SPB than in B implants. Adipose-derived SVF cells, PRP and BMM rapidly initiated osteogenesis what makes this combination promising candidate for treatment of bone defects.
Department of Biology and Human Genetics, Faculty of Medicine, University of Niš, Blvd. Dr Zoran Đinđić 81, 18000 Niš, Serbia; Department for Cell and Tissue Engineering, Faculty of Medicine, University of Niš, Blvd. Dr Zoran Đinđić 81, 18000 Niš, Serbia. Electronic address: stevo.najman@gmail.com.
Stevo J
SJ
Najman
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Višegradska 33, 18000 Niš, Serbia. Electronic address: biovlada@yahoo.com.
Vladimir J
VJ
Cvetković
Department of Biology and Human Genetics, Faculty of Medicine, University of Niš, Blvd. Dr Zoran Đinđić 81, 18000 Niš, Serbia; Department for Cell and Tissue Engineering, Faculty of Medicine, University of Niš, Blvd. Dr Zoran Đinđić 81, 18000 Niš, Serbia. Electronic address: jella82@gmail.com.
Jelena G
JG
Najdanović
Department of Biology and Human Genetics, Faculty of Medicine, University of Niš, Blvd. Dr Zoran Đinđić 81, 18000 Niš, Serbia; Department for Cell and Tissue Engineering, Faculty of Medicine, University of Niš, Blvd. Dr Zoran Đinđić 81, 18000 Niš, Serbia. Electronic address: s.sanja88@gmail.com.
Sanja
S
Stojanović
Department of Biology and Human Genetics, Faculty of Medicine, University of Niš, Blvd. Dr Zoran Đinđić 81, 18000 Niš, Serbia; Department for Cell and Tissue Engineering, Faculty of Medicine, University of Niš, Blvd. Dr Zoran Đinđić 81, 18000 Niš, Serbia. Electronic address: marijavukelic@yahoo.com.
Marija Đ
MĐ
Vukelić-Nikolić
Department of Maxillofacial Surgery, Clinic of Stomatology, Blvd. Dr Zoran Đinđić 52, 18000 Niš, Serbia. Electronic address: drivicavuckovic@gmail.com.
Ivica
I
Vučković
Department of Maxillofacial Surgery, Clinic of Stomatology, Blvd. Dr Zoran Đinđić 52, 18000 Niš, Serbia; Faculty of Medicine, University of Niš, Blvd. Dr Zoran Đinđić 81, 18000 Niš, Serbia. Electronic address: dragan-petrovic@hotmail.com.
Dragan
D
Petrović
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001808:Blood Vessels / Q000166:cytology*; D001842:Bone and Bones / Q000378:metabolism; D002478:Cells, Cultured; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009999:Ossification, Heterotopic / Q000378:metabolism*; D053657:Platelet-Rich Plasma; D060888:Real-Time Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology; D059467:Transcriptome
ADSCs; Adipose-derived SVF cells; Bone-related genes; Ectopic osteogenesis; PRP