Relationship of microRNA 616 gene polymorphism with prognosis of patients with premature coronary artery disease.
OBJECTIVE
To investigate the relationship of microRNA 616 (has-miR-616) single nucleotide polymorphisms (SNPs) and its target gene paraoxonase 1 (PON 1) with the prognosis of patients with premature coronary artery disease (pCAD).


METHODS
266 pCAD cases in the case group and 300 cases in the control group were collected. Using the polymorphism method of polymerase chain reaction and restriction fragment length, the has-miR-616 rs3735590 genotypes of target gene PON 1 were detected.


RESULTS
In the changes of rs3735590 C/T SNPs, compared with CC genotypes, the risk of coronary heart disease of the individuals carrying CT and TT genotypes were reduced by 42% and 31%, respectively (p < 0.05). The risk of developing coronary heart disease in individuals carrying CT and TT genotypes were reduced significantly in the population with levels of total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol.


CONCLUSIONS
Target gene PON 1 of hsa-miRNA-616 rs3735590C-T SNPs is associated with the reduced incidence risk of pCAD, and carrying C alleles is an independent risk factor for pCAD.
Yan
Y
Zhang
Shijie
S
Wang
Yongqin
Y
Li
Chunyan
C
Zhang
Jiahong
J
Xue
Xiaosan
X
Wu
Congxia
C
Wang
D000328:Adult; D017668:Age of Onset; D043303:Aryldialkylphosphatase / Q000235:genetics; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D002784:Cholesterol / Q000097:blood; D003324:Coronary Artery Disease / Q000453:epidemiology / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D011379:Prognosis; D018570:Risk Assessment
C584055:MIRN616 microRNA, human; D035683:MicroRNAs; D004247:DNA; D002784:Cholesterol; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human