Multidrug-Resistant Sequence Type 235 Pseudomonas aeruginosa Clinical Isolates Producing IMP-26 with Increased Carbapenem-Hydrolyzing Activities in Vietnam.
Forty clinical isolates of multidrug-resistant Pseudomonas aeruginosa were obtained in a medical setting in Hanoi, Vietnam. Whole genomes of all 40 isolates were sequenced by MiSeq (Illumina), and phylogenic trees were constructed from the single nucleotide polymorphism concatemers. Of these 40 isolates, 24 (60.0%) harbored metallo-β-lactamase-encoding genes, including blaIMP-15, blaIMP-26, blaIMP-51, and/or blaNDM-1Of these 24 isolates, 12 harbored blaIMP-26and belonged to sequence type 235 (ST235). Escherichia coli expressing blaIMP-26was significantly more resistant to doripenem and meropenem than E. coli expressing blaIMP-1and blaIMP-15IMP-26 showed higher catalytic activity against doripenem and meropenem than IMP-1 and against all carbapenems tested, including doripenem, imipenem, meropenem, and panipenem, than did IMP-15. These data suggest that clinical isolates of multidrug-resistant ST235 P. aeruginosa producing IMP-26 with increased carbapenem-hydrolyzing activities are spreading in medical settings in Vietnam.
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Tatsuya
T
Tada
Department of Microbiology, Hanoi Medical University, Hanoi, Vietnam.|Bach Mai Hospital, Hanoi, Vietnam.
Pham Hong
PH
Nhung
Pathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Tohru
T
Miyoshi-Akiyama
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Kayo
K
Shimada
Disease Control and Prevention Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Mitsuhiro
M
Tsuchiya
Bach Mai Hospital, Hanoi, Vietnam.
Doan Mai
DM
Phuong
Bach Mai Hospital, Hanoi, Vietnam.
Nguyen Quoc
NQ
Anh
Disease Control and Prevention Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
Norio
N
Ohmagari
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan tkirikae@ri.ncgm.go.jp.
Teruo
T
Kirikae
D015780:Carbapenems / Q000493:pharmacokinetics / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects* / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D014744:Vietnam; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
D015780:Carbapenems; C409201:beta-lactamase IMP-1; D001618:beta-Lactamases; C547882:beta-lactamase IMP-15, Pseudomonas aeruginosa; C552864:beta-lactamase NDM-1