Association between LEKR1-CCNL1 and IGSF21-KLHDC7A gene polymorphisms and diabetic retinopathy of type 2 diabetes mellitus in the Chinese Han population.
BACKGROUND
Diabetic retinopathy (DR) is one of the most common microvascular complications of diabetes. The present study aimed to identify a possible connection between gene polymorphisms and the risk of developing DR.


METHODS
A total of 319 patients with type 2 diabetes mellitus (T2DM) were selected. All patients underwent a complete eye examination. Based on this, the patients with T2DM were divided into two subgroups: 175 patients with retinopathy (DR) and 144 patients without retinopathy (NDR). We calculated the genotype frequencies of case and control subjects using the chi-squares test. The odds ratio (OR) and 95% confidence intervals (CIs) were calculated by unconditional logistic regression adjusted for age and sex.


RESULTS
The finding by analysis is that the mean of duration of diabetes, total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), glomerular filtration rate and C-peptide were significantly different between DR and NDR. We found significant differences in cystatin-C concentrations with LEKR1-CCNL1 rs13064954 and NOS3 rs3918227 of different genotypes. Significant differences in serum TG levels were seen among the three genotypes of MTHFR rs1537516. Subjects carried the T allele of IGSF21-KLHDC7A rs3007729 had higher serum LDL concentrations (p = 0.015). In the allele model, LEKR1-CCNL1 rs13064954 decreased the risk of DR (OR =0.57, 95% CI = 0.34-0.96, p = 0.032). Under the dominant model, the IGSF21-KLHDC7A rs3007729 CT-TT genotype increased the risk of DR (OR =1.84, 95% CI = 1.14-2.99, p = 0.013).


CONCLUSIONS
Our results suggest that LEKR1-CCNL1 and IGSF21-KLHDC7A influence the development of DR.
Department of Ophthalmology, The Inner Mongolia Autonomous Region People's Hospital, Hohhot, China. Xiaoh_lin@163.com.
Xiaohui
X
Lin
Department of Hand and Microsurgery II, the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China.
Jihong
J
Wang
Department of Ophthalmology, The Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
Lixia
L
Yun
Department of Ophthalmology, The Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
Shuhong
S
Jiang
Department of Ophthalmology, The Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
Langen
L
Li
Department of Ophthalmology, The Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
Xiaohai
X
Chen
Department of Ophthalmology, The Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
Zhen
Z
Li
Department of Ophthalmology, The Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
Qiang
Q
Lu
Department of Ophthalmology, The Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
Yihui
Y
Zhang
Department of Ophthalmology, The Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
Xiaocheng
X
Ma
D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D002681:China; D016213:Cyclins / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000208:ethnology / Q000235:genetics*; D003930:Diabetic Retinopathy / Q000208:ethnology / Q000209:etiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011506:Proteins / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*
C468873:CCNL1 protein, human; D002352:Carrier Proteins; D016213:Cyclins; C000605359:LEKR1 protein, human; D008055:Lipids; D008565:Membrane Proteins; D011506:Proteins; D044767:Ubiquitin-Protein Ligases
IGSF21-KLHDC7A; diabetic retinopathy; polymorphism LEKR1-CCNL1; type 2 diabetes mellitus