The Fimbrial Protein is a Virulence Factor and Potential Vaccine Antigen of Avibacterium paragallinarum.
Fimbriae are recognized as virulence factors and potential vaccine antigens of several pathogenic bacteria, but the function of the fimbriae from Avibacterium paragallinarum is not well known. In this study, a gene encoding the fimbrial protein FlfA was identified in A. paragallinarum . Sequencing analysis of the putative promoter region of flfA suggests that flfA expression in A. paragallinarum might be controlled by phase variation. The flfA gene from A. paragallinarum was expressed as a recombinant protein (r-FlfA) in Escherichia coli . Immunization with r-FlfA conferred chickens protection against challenge infection with A. paragallinarum . Virulence assays showed that the flfA-deficient mutants of A. paragallinarum were less virulent than their parental wild-type strains. These results indicated that the fimbrial protein FlfA is a virulence factor and potential vaccine antigen from A. paragallinarum .
A Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 40227, Taiwan.
C-C
CC
Liu
A Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 40227, Taiwan.
S-C
SC
Ou
A Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 40227, Taiwan.
D-H
DH
Tan
A Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 40227, Taiwan.
M-K
MK
Hsieh
B Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan.
J-H
JH
Shien
A Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 40227, Taiwan.|C Agricultural Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan.
P-C
PC
Chang
D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D006192:Haemophilus Infections / Q000276:immunology*; D018073:Haemophilus Vaccines / Q000276:immunology*; D044134:Haemophilus paragallinarum / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D000071497:Immunogenicity, Vaccine; D037521:Virulence Factors / Q000235:genetics* / Q000276:immunology
D001426:Bacterial Proteins; D018073:Haemophilus Vaccines; D037521:Virulence Factors
Avibacterium paragallinarum; fimbriae; vaccine; virulence