Fabrication and evaluation of novel zeolite membranes to control the neoplastic activity and anti-tumoral drug treatments in human breast cancer cells. Part 1: Synthesis and characterization of Pure Zeolite Membranes and Mixed Matrix Membranes for adhesion and growth of cancer cells.
Novel pure and hybrid zeolite membranes were prepared with appropriate different physicochemical characteristics such as frameworks, hydrophilicity, crystal size, chemical composition, acid-base properties (Point of Zero Charge, PZC) and surface morphology and used in inorganic cell/scaffold constructs. Because the control of cell interactions, as the adhesion, proliferation, remodelling and mobility, is important for differentiation and progression of tumors, this work focused on response of cancer cells adhered and grown on synthesized zeolite surfaces in order to study the influence of these scaffolds in controlled conditions. We have selected the MCF-7 and MDA-MB-231 human breast cancer cell line as model tumor cell lines. This study showed that all the zeolite membranes synthesized are excellent scaffolds because they are very selective materials to support the adhesion and growth of neoplastic cells. All zeolite scaffolds were characterized by FESEM, FTIR ATR, XRD, AFM, PZC and contact angle analyses. Cell adhesion, viability and morphology were measured by count, MTT assay and FESEM microphotography analysis, at various incubation times.
Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Cubo 4/c, 87036 Rende, Italy. Electronic address: p.tavolaro@unical.it.
Palmira
P
Tavolaro
Department Di.B.E.S.T. (Biologia, Ecologia, Scienze della Terra), Unit of Physiology, University of Calabria, Cubo 4/c, 87036 Rende, Italy.
Guglielmo
G
Martino
Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Cubo 4/c, 87036 Rende, Italy.
Sebastiano
S
Andò
Research Institute on Membrane Technology, Unit of Zeolite Membranes, ITM-CNR, University of Calabria, Cubo 17/c, 87036 Rende, Italy.
Adalgisa
A
Tavolaro
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D003460:Crystallization; D005260:Female; D006801:Humans; D008567:Membranes, Artificial; D016062:Porosity; D014961:X-Ray Diffraction; D017641:Zeolites / Q000737:chemistry*
D000970:Antineoplastic Agents; D008567:Membranes, Artificial; D017641:Zeolites
Cell adhesion; Human breast cancer cell; Mixed Matrix Membranes; Point of Zero Charge; Pure Zeolite Membranes; Scaffolds