Role of Nesfatin-1 in the Reproductive Axis of Male Rat.
Nesfatin-1 is an important molecule in the regulation of reproduction. However, its role in the reproductive axis in male animals remains to be understood. Here, we found that nesfatin-1 was mainly distributed in the arcuate nucleus (ARC), paraventricular nucleus (PVN), periventricular nucleus (PeN), and lateral hypothalamic area (LHA) of the hypothalamus; adenohypophysis and Leydig cells in male rats. Moreover, the concentrations of serum nesfatin-1 and its mRNA in hypothalamo-pituitary-gonadal axis (HPGA) vary with the age of the male rat. After intracerebroventricular injection of nesfatin-1, the hypothalamic genes for gonadotrophin releasing hormone (GnRH), kisspeptin (Kiss-1), pituitary genes for follicle-stimulate hormone β(FSHβ), luteinizing hormone β(LHβ), and genes for testicular steroidogenic acute regulatory (StAR) expression levels were decreased significantly. Nesfatin-1 significantly increased the expression of genes for 3β-hydroxysteroid dehydrogenase (3β-HSD), 17β-hydroxysteroid dehydrogenase (17β-HSD), and cytochrome P450 cleavage (P450scc) in the testis of pubertal rats, but their levels decreased in adult rats (P < 0.05), along with the serum FSH, LH, and testosterone (T) concentrations. After nesfatin-1 addition in vitro, T concentrations of the supernatant were significantly higher than that in the control group. These results were suggestive of the role of nesfatin-1 in the regulation of the reproductive axis in male rats.
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.
Xiaoxiao
X
Gao
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.
Kaifa
K
Zhang
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.
Min
M
Song
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.
Xiumei
X
Li
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.
Lei
L
Luo
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.
Yuan
Y
Tian
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.
Yunhai
Y
Zhang
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.
Yunsheng
Y
Li
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.
Xiaorong
X
Zhang
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.
Yinghui
Y
Ling
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.
Fugui
F
Fang
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Sciences and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.|Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, No. 130 of Changjiang West Road, Hefei, Anhui 230036, China.
Ya
Y
Liu
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D037201:Follicle Stimulating Hormone, beta Subunit / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism*; D007034:Hypothalamus, Posterior / Q000378:metabolism; D007985:Leydig Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000502:physiology*; D000080866:Nucleobindins; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D013737:Testis / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism
D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D037201:Follicle Stimulating Hormone, beta Subunit; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase