Taxonomic confirmation of mud crab species (genus Scylla) in Bangladesh by nuclear and mitochondrial DNA markers.
Taxonomy of mud crabs genus Scylla has been misidentified for several years due to their high morphological plasticity. Several reports concerning mud crab have been published with misleading identification in Bangladesh. In this study, partial fragments of nuclear and mitochondrial DNA of Scylla species obtained from four locations along the Bangladesh coast were used to resolve taxonomical ambiguity of mud crab species. A single PCR product from the nuclear first internal transcribed spacer (ITS-1) marker and phylogenetic trees constructed based on 16S rDNA sequences indicated that all Scylla species obtained in this study were S. olivacea. Both molecular data and morphological characters revealed that S. olivacea is the only major species in Bangladesh coastal waters. Further, the 16S rDNA haplotypes significantly differed with known S. serrata by 33%. From this study it is clear that 'S. serrata' commonly reported from Bangladesh should be S. olivacea.
a Department of Aquatic Bioscience , Graduate School of Agricultural and Life Sciences, the University of Tokyo , Bunkyo , Tokyo , Japan.|b Fisheries and Marine Resource Technology Discipline, Khulna University , Khulna , Bangladesh.
Mohammed Golam
MG
Sarower
b Fisheries and Marine Resource Technology Discipline, Khulna University , Khulna , Bangladesh.
Sheik Istiak Md
SIM
Shahriar
a Department of Aquatic Bioscience , Graduate School of Agricultural and Life Sciences, the University of Tokyo , Bunkyo , Tokyo , Japan.
Hiromasa
H
Nakamura
b Fisheries and Marine Resource Technology Discipline, Khulna University , Khulna , Bangladesh.
Muhammad Abdur
MA
Rouf
a Department of Aquatic Bioscience , Graduate School of Agricultural and Life Sciences, the University of Tokyo , Bunkyo , Tokyo , Japan.
Shigeru
S
Okada
D000818:Animals; D001459:Bangladesh; D003386:Brachyura / Q000145:classification / Q000235:genetics*; D002467:Cell Nucleus / Q000235:genetics; D005819:Genetic Markers; D008928:Mitochondria / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction
D005819:Genetic Markers
16S rDNA; Internal transcribed spacer; mud crab; phylogeny; taxonomy