The Protective Effects of Alpha-Lipoic Acid and Coenzyme Q10 Combination on Ovarian Ischemia-Reperfusion Injury: An Experimental Study.
Objective. This study aims to evaluate whether alpha-lipoic acid and/or coenzyme Q10 can protect the prepubertal ovarian tissue from ischemia-reperfusion injury in an experimental rat model of ovarian torsion. Materials and Methods. Forty-two female preadolescent Wistar-Albino rats were divided into 6 equal groups randomly. The sham group had laparotomy without torsion; the other groups had torsion/detorsion procedure. After undergoing torsion, group 2 received saline, group 3 received olive oil, group 4 received alpha-lipoic acid, group 5 received coenzyme Q10, and group 6 received both alpha-lipoic acid and coenzyme Q10 orally. The oxidant-antioxidant statuses of these groups were compared using biochemical measurement of oxidized/reduced glutathione, glutathione peroxidase and malondialdehyde, pathological evaluation of damage and apoptosis within the ovarian tissue, and immunohistochemical assessment of nitric oxide synthase. Results. The left ovaries of the alpha-lipoic acid + coenzyme Q10 group had significantly lower apoptosis scores and significantly higher nitric oxide synthase content than the left ovaries of the control groups. The alpha-lipoic acid + coenzyme Q10 group had significantly higher glutathione peroxidase levels and serum malondialdehyde concentrations than the sham group. Conclusions. The combination of alpha-lipoic acid and coenzyme Q10 has beneficial effects on oxidative stress induced by ischemia-reperfusion injury related to ovarian torsion.
Department of Pediatric Surgery, Afyon Kocatepe University Hospital, 03000 Afyonkarahisar, Turkey.
Ahmet Ali
0000-0002-2750-7317
AA
Tuncer
Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03000 Afyonkarahisar, Turkey.
Mehmet Fatih
MF
Bozkurt
Department of Biochemistry, Afyon Kocatepe University Medical Faculty, 03000 Afyonkarahisar, Turkey.
Tulay
T
Koken
Department of Biostatistics, Afyon Kocatepe University Medical Faculty, 03000 Afyonkarahisar, Turkey.
Nurhan
N
Dogan
Department of Obstetrics & Gynecology, Afyon Kocatepe University Hospital, 03000 Afyonkarahisar, Turkey.
Mine Kanat
MK
Pektaş
Department of Pediatric Surgery, Afyon Kocatepe University Hospital, 03000 Afyonkarahisar, Turkey.
Didem
D
Baskin Embleton