Triggered isothermal PCR by denaturation bubble-mediated strand exchange amplification.
Here, we introduced the concept of strand exchange amplification (SEA) mediated by denaturation bubbles. Similar to traditional PCR, it only employed a DNA polymerase and a pair of common primers to realize a three-step cycle process, but the entire SEA reaction was performed at a single temperature.
College of Life Sciences, Qingdao University, Qingdao, 266071, P. R. China.
Chao
C
Shi
Fanjin
F
Shang
Meiling
M
Zhou
Pansong
P
Zhang
Yifan
Y
Wang
Cuiping
C
Ma