Combined proteomic and metallomic analyses in Scrobicularia plana clams to assess environmental pollution of estuarine ecosystems.
Estuaries are very important ecosystems with great ecological and economic value, but usually highly impacted by anthropogenic pressure. Thus, the assessment of pollution levels in these habitats is critical in order to evaluate their environmental quality. In this work, we combined complementary metallomic and proteomic approaches with the aim to monitor the effects of environmental pollution on Scrobicularia plana clams captured in three estuarine systems from the south coast of Portugal; Arade estuary, Ria Formosa and Guadiana estuary. Multi-elemental profiling of digestive glands was carried out to evaluate the differential pollution levels in the three study areas. Then, proteomic analysis by means of two-dimensional gel electrophoresis and mass spectrometry revealed twenty-one differential proteins, which could be associated with multiple toxicological mechanisms induced in environmentally stressed organisms. Accordingly, it could be concluded that the combination of different omic approaches presents a great potential in environmental research.
Department of Chemistry, Faculty of Experimental Sciences, University of Huelva, Campus de El Carmen, 21007 Huelva, Spain; Campus of Excellence International ceiA3, University of Huelva, Spain; Research Center of Health and Environment (CYSMA), University of Huelva, Campus de El Carmen, 21007 Huelva, Spain. Electronic address: raul.gonzalez@dqcm.uhu.es.
Raúl
R
González-Domínguez
UCIBIO-REQUIMTE, Chemistry Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal; ProteoMass Scientific Society, MadanPark, Rua dos Inventores s/n, Monte de Caparica, 2829-516 Caparica, Portugal. Electronic address: hms14862@fct.unl.pt.
Hugo Miguel
HM
Santos
CIMA, Faculty of Marine and Environmental Sciences, University of Algarve, Campus de Gambelas, 8005-135 Faro, Portugal. Electronic address: mbebian@ualg.pt.
Maria João
MJ
Bebianno
Department of Chemistry, Faculty of Experimental Sciences, University of Huelva, Campus de El Carmen, 21007 Huelva, Spain; Campus of Excellence International ceiA3, University of Huelva, Spain; Research Center of Health and Environment (CYSMA), University of Huelva, Campus de El Carmen, 21007 Huelva, Spain. Electronic address: tamara.garcia@dqcm.uhu.es.
Tamara
T
García-Barrera
Department of Chemistry, Faculty of Experimental Sciences, University of Huelva, Campus de El Carmen, 21007 Huelva, Spain; Campus of Excellence International ceiA3, University of Huelva, Spain; Research Center of Health and Environment (CYSMA), University of Huelva, Campus de El Carmen, 21007 Huelva, Spain. Electronic address: ariza@dqcm.uhu.es.
José Luis
JL
Gómez-Ariza
UCIBIO-REQUIMTE, Chemistry Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal; ProteoMass Scientific Society, MadanPark, Rua dos Inventores s/n, Monte de Caparica, 2829-516 Caparica, Portugal. Electronic address: jlcapelom@gmail.com.
José Luis
JL
Capelo
D000818:Animals; D049872:Bivalvia / Q000187:drug effects / Q000378:metabolism*; D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods*; D063366:Estuaries; D019216:Metals, Heavy / Q000032:analysis* / Q000378:metabolism / Q000633:toxicity; D011174:Portugal; D040901:Proteomics / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism / Q000633:toxicity
D019216:Metals, Heavy; D014874:Water Pollutants, Chemical
Environmental pollution; Estuaries; Metallomics; Proteomics; Scrobicularia plana