Sialic acid level is significantly elevated in IgM enriched protein fraction in sera of cancer patients.
Serum sialic acid (SA) levels are important for diagnosis, follow-up, and mechanistic analysis of malignant diseases. However, little is known about the levels of SA bound to serum IgM. Here, we isolated IgM from sera of healthy individuals and patients with cancer using DEAE chromatography and 8% polyethylene glycol precipitation. In this fraction, which contained partially purified IgM (recovery; 52%; purity: 25%), SA was quantified with fluorescence detection-HPLC (detection limit: 0.08 μM). SA levels in the IgM-enriched fraction was significantly higher in cancer patients (104 ± 27 µM) than in healthy individuals (81 ± 11 µM; P = 0.003).
a Torii Clinic Medical Corp. Hospitals & Clinics , Maizuru City , Japan.
Tsuyoshi
T
Torii
b Laboratory of Public Health and Environmental Chemistry , Kyoto Biseibutsu Kenkyusho, Kyoto , Japan.
Kentaku
K
Kanemitsu
c Regenerative Medicine and Genetic Information Laboratory, Department of Biomedical Information, Faculty of Life and Medical Sciences , Doshisha University , Kyoto , Japan.
Akeo
A
Hagiwara
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood* / Q000302:isolation & purification*; D019158:N-Acetylneuraminic Acid / Q000097:blood*; D009369:Neoplasms / Q000097:blood* / Q000175:diagnosis; D011092:Polyethylene Glycols / Q000737:chemistry
D007075:Immunoglobulin M; D011092:Polyethylene Glycols; D019158:N-Acetylneuraminic Acid
DAEA ion-exchange chromatography; IgM antibodies; human serum; sialic acid