The effect of tadalafil therapy on kidney damage caused by sepsis in a polymicrobial septic model induced in rats: a biochemical and histopathological study.
BACKGROUND
Sepsis is an inflammatory reaction to bacteria involving the whole body and is a significant cause of mortality and economic costs. The purpose of this research was to determine whether tadalafil exhibits a preventive effect on sepsis in a septic model induced in rats with cecal ligation and puncture (CLP).


METHODS
Rats were randomly separated into groups, 10 rats in each: (i) a sham (control) group, (ii) an untreated sepsis group, (iii) a sepsis group treated with 5mg/kg tadalafil and (iv) a sepsis group treated with 10mg/kg tadalafil. A polymicrobial sepsis model was induced in rats using CLP. Rats were sacrificed after 16h, and blood and kidney tissues were collected for biochemical and histopathological study.


RESULTS
Levels of the inflammatory parameter IL-6 decreased significantly in the sepsis groups receiving tadalafil in comparison with the untreated sepsis group (p < 0.05). In terms of histopathology, inflammation scores investigated in kidney tissues decreased significantly in the sepsis groups receiving tadalafil compared to the untreated sepsis group (p < 0.05). In addition, levels of creatinine and cystatin C measured in septic rats receiving tadalafil were lower by a clear degree than in septic rats (p < 0.05).


CONCLUSIONS
In this study, tadalafil exhibited a preventive effect for sepsis-related damage by suppressing inflammation in serum and kidney tissue of septic rats in a polymicrobial sepsis model induced with CLP.
Department of Urology, Faculty of Medicine, Ordu University, Ordu, Turkey.
Erdal
E
Benli
Department of Biochemistry, Faculty of Medicine, Ordu University, Ordu, Turkey.
Sema Nur
SN
Ayyildiz
Department of Physiology, Faculty of Medicine, Ordu University, Ordu, Turkey.
Selma
S
Cirrik
Department of Histolology, Faculty of Medicine, Ordu University, Ordu, Turkey.
Sibel
S
Koktürk
Department of Urology, Faculty of Medicine, Ordu University, Ordu, Turkey.
Abdullah
A
Cirakoglu
Department of Biochemistry, Faculty of Medicine, Ordu University, Ordu, Turkey.
Tevfik
T
Noyan
Department of Urology, Faculty of Medicine, Ordu University, Ordu, Turkey.
Ali
A
Ayyildiz
Department of Urology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey.
Cankon
C
Germiyanoglu
D000818:Animals; D002116:Calcitonin / Q000097:blood; D002374:Catalase / Q000032:analysis; D003404:Creatinine / Q000097:blood; D055316:Cystatin C / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000097:blood; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008026:Ligation; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009195:Peroxidase / Q000032:analysis; D058986:Phosphodiesterase 5 Inhibitors / Q000627:therapeutic use*; D011897:Random Allocation; D017208:Rats, Wistar; D012016:Reference Values; D051437:Renal Insufficiency / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control*; D015203:Reproducibility of Results; D018805:Sepsis / Q000150:complications* / Q000473:pathology / Q000517:prevention & control*; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000032:analysis; D000068581:Tadalafil / Q000627:therapeutic use*
D055316:Cystatin C; D015850:Interleukin-6; D058986:Phosphodiesterase 5 Inhibitors; D008315:Malondialdehyde; D000068581:Tadalafil; D002116:Calcitonin; D003404:Creatinine; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase
Acute Kidney Injury; Renal Insufficiency; Tadalafil