Genomic characterization and pathogenicity of a strain of type 1 porcine reproductive and respiratory syndrome virus.
The emergence of type 1 porcine reproductive and respiratory syndrome virus (PRRSV) has been noticed recently in China. In the present study, the complete genomic characterization of a strain of type 1 PRRSV (designated GZ11-G1) was described and its pathogenicity for piglets was analyzed. The results showed that the complete genome of GZ11-G1 with a size of 15,094 nt, excluding the poly (A) tails, shared 80.2-96.3% identity with the representative strains of type 1 PRRSV, and in particular, it had highest homology (96.3%) with Amervac PRRS, a live vaccine virus of type 1 PRRSV and SHE, a rescued virus from an infectious clone of Amervac PRRS virus. Compared with the vaccine virus, the nonstructural and structural proteins of GZ11-G1 displayed extensive amino acid variations except for its ORF5a. GZ11-G1 was clustered with the strains of type 1 PRRSV including Cresa3267, Cresa3249, Cresa3256, Olot/91, 9625/2012, ESP-1991-Olot91 and Amervac PRRS vaccine virus by further phylogenetic analysis. Moreover, GZ11-G1 was shown to cause fever, higher viremia and lung and lymph node lesions in piglets. Our findings indicate that GZ11-G1 is genetically related to type 1 PRRSV strains within the cluster formed by Cresa3267, Cresa3249, Cresa3256, Olot/91, 9625/2012, ESP-1991-Olot91 and Amervac PRRS vaccine virus, and it is a pathogenic for piglets. This study aids in understanding the genetic variation and evolution of type 1 PRRSV.
Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China.
Xingchen
X
Wang
Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China.
Xiaorong
X
Yang
Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China.
Rong
R
Zhou
Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China.
Lei
L
Zhou
Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China.
Xinna
X
Ge
Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China. Electronic address: guoxin@cau.edu.cn.
Xin
X
Guo
Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China. Electronic address: yanghanchun1@cau.edu.cn.
Hanchun
H
Yang
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014644:Genetic Variation; D016679:Genome, Viral; D023281:Genomics; D016366:Open Reading Frames; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D012367:RNA, Viral; D013552:Swine; D019562:Viral Load
D012367:RNA, Viral
Genome; Pathogenicity; Porcine reproductive and respiratory syndrome virus (PRRSV); Type 1