Artepillin C, a Typical Brazilian Propolis-Derived Component, Induces Brown-Like Adipocyte Formation in C3H10T1/2 Cells, Primary Inguinal White Adipose Tissue-Derived Adipocytes, and Mice.
Induction of brown-like adipocytes (beige/brite cells) in white adipose tissue (WAT) suggests a new approach for preventing and treating obesity via induction of thermogenesis associated with uncoupling protein 1 (UCP1). However, whether diet-derived factors can directly induce browning of white adipocytes has not been well established. In addition, the underlying mechanism of induction of brown-like adipocytes by diet-derived factors has been unclear. Here, we demonstrate that artepillin C (ArtC), which is a typical Brazilian propolis-derived component, significantly induces brown-like adipocytes in murine C3H10T1/2 cells and primary inguinal WAT (iWAT)-derived adipocytes. This significant induction is due to activation of peroxisome proliferator-activated receptor γ and stabilization of PRD1-BF-1-RIZ1 homologous domain-containing protein-16 (PRDM16). Furthermore, the oral administration of ArtC (10 mg/kg) for 4 weeks significantly induced brown-like adipocytes accompanied by significant expression of UCP1 and PRDM16 proteins in iWAT of mice, and was independent of the β3-adrenergic signaling pathway via the sympathetic nervous system. These findings may provide insight into browning of white adipocytes including the molecular mechanism mediated by dietary factors and demonstrate that ArtC has a novel biological function with regard to increasing energy expenditure by browning of white adipocytes.
College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487-8501, Japan.
Sho
S
Nishikawa
College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487-8501, Japan.
Hiroki
H
Aoyama
College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487-8501, Japan.
Misa
M
Kamiya
College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487-8501, Japan.
Jun
J
Higuchi
College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487-8501, Japan.
Aiko
A
Kato
College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487-8501, Japan.
Minoru
M
Soga
College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487-8501, Japan.
Taeko
T
Kawai
Department of Food and Nutritional Sciences, University of Shizuoka, Suruga-ku, Shizuoka, Shizuoka 422-8526, Japan.
Kazuki
K
Yoshimura
Department of Food and Nutritional Sciences, University of Shizuoka, Suruga-ku, Shizuoka, Shizuoka 422-8526, Japan.
Shigenori
S
Kumazawa
College of Bioscience and Biotechnology, Chubu University, Kasugai, Aichi 487-8501, Japan.
Takanori
T
Tsuda
D052437:Adipocytes, Brown / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D052438:Adipocytes, White / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D052436:Adipose Tissue, White / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D019440:Anti-Obesity Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects* / Q000235:genetics; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D047495:PPAR gamma / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D010666:Phenylpropionates / Q000302:isolation & purification / Q000494:pharmacology*; D061251:Primary Cell Culture; D011429:Propolis / Q000737:chemistry; D015398:Signal Transduction; D022722:Thermogenesis / Q000187:drug effects / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000071256:Uncoupling Protein 1 / Q000819:agonists / Q000235:genetics / Q000378:metabolism
D019440:Anti-Obesity Agents; D004268:DNA-Binding Proteins; D047495:PPAR gamma; D010666:Phenylpropionates; C521133:Prdm16 protein, mouse; D014157:Transcription Factors; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1; C102259:artepillin C; D011429:Propolis