Growth modelling of Nitrosomonas europaea ATCC® 19718 and Nitrobacter winogradskyi ATCC® 25391: A new online indicator of the partial nitrification.
The aim of the present work was to study the growth of two nitrifying bacteria. For modelling the nitrifying subsystem of the MELiSSA loop, Nitrosomonas europaea ATCC® 19718 and Nitrobacter winogradskyi ATCC® 25931 were grown separately and in cocultures. The kinetic parameters of a stoichiometric mass balanced Pirt model were identified: μmax=0.054h(-1), decay rate b=0.003h(-1) and maintenance rate m=0.135gN-NH4(+)·gX(-1)·h(-1) for Nitrosomonas europaea; μmax=0.024h(-1), b=0.001h(-1) and m=0.467gN-NO2(-)·gX(-1)·h(-1) for Nitrobacter winogradskyi. A predictive structured model of nitrification in co-culture was developed. The online evolution of the addition of KOH is correlated to the nitritation; the dissolved oxygen concentration is correlated to both nitritation and nitratation. The model suitably represents these two variables so that transient partial nitrification is assessed. This is a clue for avoiding partial nitrification by predictive functional control.
Université Clermont Auvergne, Institut Pascal, UMR CNRS 6602, TSA 60026, CS 60026, F-63178 Aubière cedex, France.
Nelly
N
Cruvellier
Université Clermont Auvergne, Institut Pascal, UMR CNRS 6602, TSA 60026, CS 60026, F-63178 Aubière cedex, France. Electronic address: laurent.poughon@univ-bpclermont.fr.
Laurent
L
Poughon
Université Clermont Auvergne, Institut Pascal, UMR CNRS 6602, TSA 60026, CS 60026, F-63178 Aubière cedex, France.
Catherine
C
Creuly
Université Clermont Auvergne, Institut Pascal, UMR CNRS 6602, TSA 60026, CS 60026, F-63178 Aubière cedex, France.
C-Gilles
CG
Dussap
ESTEC, European Space Agency, Noordwijk, The Netherlands.
Christophe
C
Lasseur
D001419:Bacteria; D019149:Bioreactors; D018920:Coculture Techniques; D007700:Kinetics; D008962:Models, Theoretical; D058465:Nitrification; D009576:Nitrobacter / Q000254:growth & development* / Q000378:metabolism; D009606:Nitrosomonas / Q000254:growth & development; D043370:Nitrosomonas europaea / Q000254:growth & development* / Q000378:metabolism
Fed-batch cultures; Life support systems; Modelling growth kinetics; Nitrobacter winogradskyi ATCC® 25931; Nitrosomonas europaea ATCC® 19718