A Derivative Method with Free Radical Oxidation to Predict Resveratrol Metabolites by Tandem Mass Spectrometry.
In this study, we demonstrated an oxidative method with free radical to generate 3,5,4'-trihydroxy-trans-stilbene (trans-resveratrol) metabolites and detect sequentially by an autosampler coupling with liquid chromatography electrospray ionization tandem mass spectrometer (LC-ESI-MS/MS). In this oxidative method, the free radical initiator, ammonium persulfate (APS), was placed in a sample bottle containing resveratrol to produce oxidative derivatives, and the reaction progress was tracked by autosampler sequencing. Resveratrol, a natural product with purported cancer preventative qualities, produces metabolites including dihydroresveratrol, 3,4'-dihydroxy-trans-stilbene, lunularin, resveratrol monosulfate, and dihydroresveratrol monosulfate by free radical oxidation. Using APS free radical, the concentrations of resveratrol derivatives differ as a function of time. Besides simple, convenient and time- and labor saving, the advantages of free radical oxidative method of itsin situgeneration of oxidative derivatives followed by LC-ESI-MS/MS can be utilized to evaluate different metabolites in various conditions.
Department of Biotechnology, Kaohsiung Medical University, Kaohsiung,Taiwan;
Wangta
W
Liu
Institute of  Biomedical Science, National Sun Yat-Sen University, Kaohsiung,Taiwan;
Yow-Ling
YL
Shiue
Department of Biotechnology, Fooyin University, Kaohsiung,Taiwan;
Yi-Reng
YR
Lin
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung,Taiwan;; Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; 6Department of  Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; 7Center for Resources, Research and Development, Kaohsiung Medical University, Kaohsiung,Taiwan;
Hugo You-Hsien
HY
Lin
Department of Biotechnology, Kaohsiung Medical University, Kaohsiung,Taiwan;; Institute of  Biomedical Science, National Sun Yat-Sen University, Kaohsiung,Taiwan;;
Shih-Shin
SS
Liang
Free radical; ammonium persulfate (APS); liquid chromatography tandem mass spectrometry (LC–MS/MS); metabolite; multiple reaction monitoring (MRM); resveratrol