Biomolecular detection at ssDNA-conjugated nanoparticles by nano-impact electrochemistry.
We describe the use of ssDNA functionalized silver nanoparticle (AgNP) probes for quantitative investigation of biorecognition and real time detection of biomolecular targets using nano-impact electrochemistry. The method is based on measurements of the individual collision events between ssDNA aptamer-functionalized AgNPs and a carbon fiber miroelectrode (CFME). Specific binding events of target analyte induced collision frequency changes enabling ultrasensitive detection of the aptamer target in a single step. These changes are assigned to the surface coverage of the NP by the ssDNA aptamers and subsequent conformational changes of the aptamer probe which affect the electron transfer between the NP and the electrode surface. The method enables sensitive and selective detection of ochratoxin A (OTA), chosen here as a model target, with a limit of detection of 0.05nM and a relative standard deviation of 4.9%. The study provides a means of characterizing bioconjugation of AgNPs with aptamers and assessing biomolecular recognition events with high sensitivity and without the use of exogenous reagents or enzyme amplification steps. This methodology can be broadly applicable to other bioconjugated systems, biosensing and related bioanalytical applications.
Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA.
Anahita
A
Karimi
Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA.
Akhtar
A
Hayat
Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA. Electronic address: eandrees@clarkson.edu.
Silvana
S
Andreescu
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D005504:Food Analysis / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D009793:Ochratoxins / Q000032:analysis*; D012834:Silver / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D009793:Ochratoxins; C025589:ochratoxin A; D012834:Silver
Aptasensor; Bioconjugation; Biomolecular recognition; Impact electrochemistry; Nanoparticles; Target detection