Mechanical and antibacterial properties of a nanocellulose-polypyrrole multilayer composite.
In this study, a composite film based on TEMPO-oxidized cellulose nanofibers (TOCN), polyvinyl alcohol (PVA) and polypyrrole (PPy) was synthesized in situ by a chemical polymerization, resulting in the induced absorption of PPy on the surface of the TOCN. The composite films were investigated with scanning electron microscopy, thermogravimetric analysis, contact angle measurements, mechanical tests, and evaluation of antibacterial properties. The developed composite has nearly identical Young modulus (3.4GPa), elongation (2.6%) and tensile stress (about 51MPa) to TOCN even if PPy, which as poor properties by itself, was incorporated. From the energy-dispersive X-ray spectroscopy (EDX) results, it was shown that PPy is mainly located on the composite surface. Results confirmed by an increase from 54.5 to 83° in contact angle, an increased heat protection (Thermogravimetric analysis) and a decrease in surface energy. The nanocomposites were also evaluated for antibacterial activity against bacteria occasionally found in food: Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli). The results indicate that the nanocomposites are effective against all of the bacteria studied as shown by the decrease of 5.2logcolonyformingunits (CFU) for B. subtilis and 6.5logCFU for E. coli. Resulting in the total destruction of the studied bacteria. The perfect match between the resulting inhibition zone and the composite surface area has demonstrated that our composite was contact active with a slight leaching of PPy. Our composite was successful as an active packaging on meat (liver) as bacteria were killed by contact, thereby preventing the spread of possible diseases. While it has not been tested on bacteria found in medicine, TOCN/PVA-PPy film may be able to act as an active sterile packaging for surgical instruments.
Lignocellulosic Material Research Center, Université du Québec à Trois-Rivières, 3351 boul. des Forges, C.P. 500, Trois-Rivières, QC G9A 5H7, Canada. Electronic address: Benoit.bideau@uqtr.ca.
Benoit
B
Bideau
Univ. Grenoble Alpes, LGP2, F-38000 Grenoble, France; CNRS, LGP2, F-38000 Grenoble, France. Electronic address: Julien.bras@pagora.grenoble-inp.fr.
Julien
J
Bras
Univ. Grenoble Alpes, LGP2, F-38000 Grenoble, France; CNRS, LGP2, F-38000 Grenoble, France. Electronic address: Seema.Saini@lgp2.grenoble-inp.fr.
Seema
S
Saini
Lignocellulosic Material Research Center, Université du Québec à Trois-Rivières, 3351 boul. des Forges, C.P. 500, Trois-Rivières, QC G9A 5H7, Canada. Electronic address: Claude.daneault@uqtr.ca.
Claude
C
Daneault
Lignocellulosic Material Research Center, Université du Québec à Trois-Rivières, 3351 boul. des Forges, C.P. 500, Trois-Rivières, QC G9A 5H7, Canada. Electronic address: Eric.Loranger1@uqtr.ca.
Eric
E
Loranger
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002482:Cellulose / Q000737:chemistry*; D003497:Cyclic N-Oxides / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects; D055595:Mechanical Phenomena; D008826:Microbial Sensitivity Tests; D057139:Nanofibers / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D034421:Sus scrofa; D013696:Temperature; D013818:Thermogravimetry
D000900:Anti-Bacterial Agents; D003497:Cyclic N-Oxides; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D011142:Polyvinyl Alcohol; D002482:Cellulose; C003959:TEMPO
Antibacterial; Composite; Mechanical; Nanofibers cellulose; Polypyrrole