Cell- and region-specific expression of depression-related protein p11 (S100a10) in the brain.
P11 (S100a10), a member of the S100 family of proteins, has widespread distribution in the vertebrate body, including in the brain, where it has a key role in membrane trafficking, vesicle secretion, and endocytosis. Recently, our laboratory has shown that a constitutive knockout of p11 (p11-KO) in mice results in a depressive-like phenotype. Furthermore, p11 has been implicated in major depressive disorder (MDD) and in the actions of antidepressants. Since depression affects multiple brain regions, and the role of p11 has only been determined in a few of these areas, a detailed analysis of p11 expression in the brain is warranted. Here we demonstrate that, although widespread in the brain, p11 expression is restricted to distinct regions, and specific neuronal and nonneuronal cell types. Furthermore, we provide comprehensive mapping of p11 expression using in situ hybridization, immunocytochemistry, and whole-tissue volume imaging. Overall, expression spans multiple brain regions, structures, and cell types, suggesting a complex role of p11 in depression. J. Comp. Neurol. 525:955-975, 2017. © 2016 Wiley Periodicals, Inc.
Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York, USA.
Ana
A
Milosevic
Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York, USA.
Thomas
T
Liebmann
Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York, USA.
Margarete
M
Knudsen
Section for Translational Neuropharmacology, Department of Clinical Neuroscience, CMM L8:01, Karolinska Institutet, 17176 Stockholm, Sweden.
Nicoletta
N
Schintu
Section for Translational Neuropharmacology, Department of Clinical Neuroscience, CMM L8:01, Karolinska Institutet, 17176 Stockholm, Sweden.
Per
P
Svenningsson
Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York, USA.
Paul
P
Greengard
D000818:Animals; D017306:Annexin A2 / Q000096:biosynthesis*; D001921:Brain / Q000378:metabolism*; D003863:Depression / Q000378:metabolism; D005260:Female; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009418:S100 Proteins / Q000096:biosynthesis*; D000099248:S100 Calcium Binding Protein A10
D017306:Annexin A2; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10
anti-Aldh1L1 antibody RRID: AB_10712968; anti-CNPase antibody RRID: AB_10122287; anti-GFAP antibody RRID: AB_305808; anti-GFP antibody RRID: AB_10000240; anti-GFP antibody RRID: AB_10073917; anti-GFP antibody RRID: AB_300798; anti-GFP antibody RRID: AB_303395; anti-Iba1 antibody RRID: AB_839504; anti-NG2 antibody RRID: AB_177646; anti-NeuN antibody RRID: AB_2298772; anti-Olig2 antibody RRID: AB_10861310; anti-p11 antibody RRID: AB_2183469; depression; endothelial cells; ependymal cells; expression mapping; glia; meninges; neurons; p11; region-specificity