Dehydration rate determines the degree of membrane damage and desiccation tolerance in bryophytes.
Desiccation tolerant (DT) organisms are able to withstand an extended loss of body water and rapidly resume metabolism upon rehydration. This ability, however, is strongly dependent on a slow dehydration rate. Fast dehydration affects membrane integrity leading to intracellular solute leakage upon rehydration and thereby impairs metabolism recovery. We test the hypothesis that the increased cell membrane damage and membrane permeability observed under fast dehydration, compared with slow dehydration, is related to an increase in lipid peroxidation. Our results reject this hypothesis because following rehydration lipid peroxidation remains unaltered, a fact that could be due to the high increase of NO upon rehydration. However, in fast-dried samples we found a strong signal of red autofluorescence upon rehydration, which correlates with an increase in ROS production and with membrane leakage, particularly the case of phenolics. This could be used as a bioindicator of oxidative stress and membrane damage.
Centre for Ecology, Evolution and Environmental Changes (CE3c), Faculdade de Ciências, Universidade de Lisboa, Lisboa, 1749-016, Portugal.
Ricardo
http://orcid.org/0000-0003-4612-9013
R
Cruz de Carvalho
Biología Celular, Dpto. Biología y Geología, Física y Química Inorgánica (ESCET), Universidad Rey Juan Carlos, Móstoles, Spain.
Myriam
M
Catalá
Centre for Ecology, Evolution and Environmental Changes (CE3c), Faculdade de Ciências, Universidade de Lisboa, Lisboa, 1749-016, Portugal.
Cristina
C
Branquinho
BioISI, Biosystems and Integrative Sciences Institute and Departamento de Biologia Vegetal, Faculdade de Ciências, Universidade de Lisboa, Lisboa, 1749-016, Portugal.
Jorge
J
Marques da Silva
Botánica & ICBIBE, Universitat de València, Valencia, Spain.
Eva
E
Barreno
D044002:Bryophyta / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology*; D003681:Dehydration; D003890:Desiccation; D005453:Fluorescence; D062385:Hydroxybenzoates / Q000032:analysis; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D018520:Plant Shoots / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological; D014867:Water / Q000502:physiology*
D062385:Hydroxybenzoates; D017382:Reactive Oxygen Species; D014867:Water; C017616:phenolic acid; D010100:Oxygen