Immunological studies of cerebrospinal fluid from patients with CNS symptoms after human papillomavirus vaccination.
In 32 patients with prolonged central nervous system symptoms after human papillomavirus (HPV) vaccination, we measured conventional and immunological markers in cerebrospinal fluid (CSF) and compared with the levels in disease controls. Our studies revealed significantly decreased chloride and neuron-specific enolase (NSE) levels in CSF of patients with CNS symptoms after HPV vaccination compared to disease controls. IL-4, IL-13, and CD4(+) T cells increased significantly in patients, and IL-17 increased significantly from 12 to 24months after symptom onset. Chemokines (IL-8 and MCP-1) were also elevated, but CD8(+) T cells, PDGF-bb and IL-12 were reduced. Antibodies to GluN2B-NT2, GluN2B-CT and GluN1-NT increased significantly. These results suggest biological, mainly immunological, changes in the CSF of patients after HPV vaccination.
National Epilepsy Center, Shizuoka Institute of Epilepsy and Neurological Disorder, NHO, 886 Urushiyama, Aoi-ward, Shizuoka, Japan; Department of Pediatrics, Gifu University School of Medicine, Yanagido, Gifu, Japan. Electronic address: takahashi-ped@umin.ac.jp.
Yukitoshi
Y
Takahashi
National Epilepsy Center, Shizuoka Institute of Epilepsy and Neurological Disorder, NHO, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: taka-m56@shizuokamind.org.
Takashi
T
Matsudaira
National Epilepsy Center, Shizuoka Institute of Epilepsy and Neurological Disorder, NHO, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: hinakano@shizuokamind.org.
Hitoshi
H
Nakano
National Epilepsy Center, Shizuoka Institute of Epilepsy and Neurological Disorder, NHO, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: evo10bibi@gmail.com.
Hirosato
H
Nasu
National Epilepsy Center, Shizuoka Institute of Epilepsy and Neurological Disorder, NHO, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: h-ikeda@shizuokamind.org.
Hitoshi
H
Ikeda
National Epilepsy Center, Shizuoka Institute of Epilepsy and Neurological Disorder, NHO, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: k-naka@shizuokamind.org.
Kentaro
K
Nakaoka
Department of Pediatrics, Hokkaido Medical Center for Child Health and Rehabilitation, 1-1-240 Kanayama, Teine-ward, Sapporo, Japan. Electronic address: takayama921@gmail.com.
Rumiko
R
Takayama
Department of Pediatrics, JA Toride Medical Center, 2-1-1, Hongo, Toride, Japan. Electronic address: fuemiyasu@k7.dion.ne.jp.
Masayasu
M
Oota
D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000134:cerebrospinal fluid; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D002648:Child; D016207:Cytokines / Q000134:cerebrospinal fluid*; D005260:Female; D053804:Granzymes / Q000134:cerebrospinal fluid; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000134:cerebrospinal fluid; D053918:Papillomavirus Vaccines / Q000009:adverse effects*; D010751:Phosphopyruvate Hydratase / Q000134:cerebrospinal fluid; D016194:Receptors, N-Methyl-D-Aspartate / Q000276:immunology; D018709:Statistics, Nonparametric; D014611:Vaccination / Q000009:adverse effects*; D055815:Young Adult
D001323:Autoantibodies; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; C121001:NR2B NMDA receptor; D053918:Papillomavirus Vaccines; D016194:Receptors, N-Methyl-D-Aspartate; D053804:Granzymes; D010751:Phosphopyruvate Hydratase
Autonomic disorder; Cognitive disorder; Human papillomavirus vaccine; Motor disorder; Sensory disorder