Investigation of the chemical markers for experiential quality evaluation of crude aconite by UHPLC-Q-TOF-MS.
Many foods and herbs are experientially classified into different commodity grades in commercial circulation. Regarding the hypertoxic herb aconite, large samples are considered to be of better quality. However, this experiential classification lacks a scientific basis. In this study, we focused on the quality diversity among different grades and studied it using the minimum lethal dose assay and a novel ultra high performance liquid chromatography coupled with time-of-flight mass spectrometry method. Toxicity assay result suggested grade I aconite had the lowest toxicity (p < 0.05). Using this method with partial least squares-discriminant analysis, we discovered nine chemomarkers, including neoline, songorine, fuziline, mesaconitine, talatizidine, dexyaconitine, talatisamine, hypaconitine, and fuzitine. Considering their toxicity and activity, we found the levels of toxic ingredients hypaconitine, dexyaconitine, and mesaconitine in grade I were lower than those in grade II (p < 0.01), while the levels of efficacy ingredients songorine, talatisamine, and neoline were the highest in grade I (p < 0.01). Further study demonstrated that the quality variation was associated with plant tissue development and toxic ingredient distribution law. Our results provide scientific evidence for the experiential quality evaluation of aconite, and it will be of great utility for other foods and herbs.
China Military Institute of Chinese Medicine, Beijing, PR China.|Chengdu University of Traditional Chinese Medicine, Chengdu, PR China.
Ding-Kun
DK
Zhang
China Military Institute of Chinese Medicine, Beijing, PR China.|Chengdu University of Traditional Chinese Medicine, Chengdu, PR China.
Xue
X
Han
China Military Institute of Chinese Medicine, Beijing, PR China.|Chengdu University of Traditional Chinese Medicine, Chengdu, PR China.
Rui-Yu
RY
Li
China Military Institute of Chinese Medicine, Beijing, PR China.
Ming
M
Niu
China Military Institute of Chinese Medicine, Beijing, PR China.|Chengdu University of Traditional Chinese Medicine, Chengdu, PR China.
Qin
Q
Dong
Jiangxi University of Traditional Chinese Medicine, Nanchang, PR China.
Ming
M
Yang
China Military Institute of Chinese Medicine, Beijing, PR China.|State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu, PR China.
Jia-Bo
JB
Wang
Integrative Medical Center, Beijing, PR China.
Xiao-He
XH
Xiao
D031841:Aconitum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D013058:Mass Spectrometry; D011786:Quality Control
D004365:Drugs, Chinese Herbal
Aconite; Mass spectrometry; Quality evaluation; Toxicity