Protein-bound polysaccharides from Coriolus versicolor attenuate LPS-induced synthesis of pro-inflammatory cytokines and stimulate PBMCs proliferation.
Protein-bound polysaccharides (PBP) isolated from Coriolus versicolor (CV) are classified as biological response modifiers capable of exhibiting various biological activities, such as anti-tumour and immunopotentiating activity. Since we have found in vivo studies that the tested PBP induced prolongation of endotoxin fever in rats, the aim of the present study was to investigate the in vitro effect of the PBP on the production of pro-inflammatory cytokines by the lipolysaccharide (LPS)-stimulated rat peripheral blood mononuclear cells (PBMCs). The results showed that the PBP affect the immunomodulating properties of the LPS-treated PBMCs by the enhancement of mitogenic activity and attenuation of the LPS-induced production of interleukin (IL)-1β and IL-6. Moreover, the tested polysaccharides peptides themselves also exhibit immunomodulatory properties manifested in the increased cell proliferation and pro-inflammatory cytokine release from PBMCs. The effect of PBP on the both phenomena was time-dependent and occurred in the U-shaped dose response manner. These findings are significant when considering the use of commercially available PBP from CV extract by cancer patients suffering from immunodeficiency, who may experience microbial infections during therapy.
Department of Immunology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University,1 Lwowska Street, 87-100 Torun, Poland. Electronic address: tomaszj@umk.pl.
Tomasz
T
Jędrzejewski
Department of Immunology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University,1 Lwowska Street, 87-100 Torun, Poland. Electronic address: m_pawlikowska@umk.pl.
Małgorzata
M
Pawlikowska
Department of Immunology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University,1 Lwowska Street, 87-100 Torun, Poland. Electronic address: piotrowski_jak@umk.pl.
Jakub
J
Piotrowski
Department of Immunology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University,1 Lwowska Street, 87-100 Torun, Poland. Electronic address: wkozak@umk.pl.
Wiesław
W
Kozak
D000818:Animals; D001487:Basidiomycota / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D062610:Fungal Polysaccharides / Q000276:immunology*; D056747:Immunomodulation; D018836:Inflammation Mediators / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology*; D051381:Rats
D016207:Cytokines; D062610:Fungal Polysaccharides; D018836:Inflammation Mediators; D008070:Lipopolysaccharides
Coriolus versicolor; Cytokines; Lipopolysaccharide; Peripheral blood mononuclear cells; Protein-bound polysaccharides