Effect of quercetin and resveratrol co-incorporated in liposomes against inflammatory/oxidative response associated with skin cancer.
The present investigation reports the development of liposomes for the co-delivery of naturally occurring polyphenols, namely quercetin and resveratrol. Small, spherical, uni/bilamellar vesicles were produced, as demonstrated by light scattering, cryo-TEM, SAXS. The incorporation of quercetin and resveratrol in liposomes did not affect their intrinsic antioxidant activity, as DPPH radical was almost completely inhibited. The cellular uptake of the polyphenols was higher when they were formulated in liposomes, and especially when co-loaded rather than as single agents, which resulted in a superior ability to scavenge ROS in fibroblasts. The in vivo efficacy of the polyphenols in liposomes was assessed in a mouse model of skin lesion. The topical administration of liposomes led to a remarkable amelioration of the tissue damage, with a significant reduction of oedema and leukocyte infiltration. Therefore, the proposed approach based on polyphenol vesicular formulation may be of value in the treatment of inflammation/oxidative stress associated with pre-cancerous/cancerous skin lesions.
Dept. of Science della Vita e dell'Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy. Electronic address: caddeoc@unica.it.
Carla
C
Caddeo
Dept. of Pharmacy and Pharmaceutical Technology, University of Valencia, Avda Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain; Institute of Molecular Recognition and Technological Development, Inter-Universitary Institute from Polytechnic University of Valencia and University of Valencia, Spain.
Amparo
A
Nacher
Dept. of Science, University of Basilicata, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy.
Antonio
A
Vassallo
Dept. of Science, University of Basilicata, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy.
Maria Francesca
MF
Armentano
Dept. of Tecnologia Química i de Tensioactius, Institut de Química Avançada de Catalunya (IQAC-CSIC), 08034 Barcelona, Spain.
Ramon
R
Pons
Nanomalaria Group, Institute for Bioengineering of Catalonia (IBEC), Baldiri Reixac 10-12, Barcelona E08028, Spain; Barcelona Institute for Global Health (ISGlobal), Barcelona Centre for International Health Research (CRESIB, Hospital Clínic-Universitat de Barcelona), Rosselló 149-153, Barcelona E08036, Spain.
Xavier
X
Fernàndez-Busquets
Dept. of Scienze del Farmaco, University of Catania, Viale A. Doria 6, 95125 Catania, Italy.
Claudia
C
Carbone
Dept. of Science della Vita e dell'Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
Donatella
D
Valenti
Dept. of Science della Vita e dell'Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
Anna Maria
AM
Fadda
Dept. of Science della Vita e dell'Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
Maria
M
Manconi
D000287:Administration, Topical; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004338:Drug Combinations; D016503:Drug Delivery Systems; D000067493:Dynamic Light Scattering; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D008081:Liposomes; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D011794:Quercetin / Q000008:administration & dosage* / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D012878:Skin Neoplasms / Q000188:drug therapy / Q000473:pathology; D013267:Stilbenes / Q000008:administration & dosage* / Q000494:pharmacology
D000975:Antioxidants; D004338:Drug Combinations; D008081:Liposomes; D017382:Reactive Oxygen Species; D013267:Stilbenes; D011794:Quercetin; D000077185:Resveratrol
Antioxidant; Fibroblast; Liposome; Quercetin; Resveratrol; Skin lesion