Structural properities and immunoenhancement of an exopolysaccharide produced by Phellinus pini.
Phellinus pini exopolysaccharide (PPE) is a mannan produced by P. pini when cultured in potato dextroseagar medium. Partial hydrolysis with acid, periodate oxidation and Smith degradation, methylation and13C nuclear magnetic resonance (NMR) spectroscopy were used to elaborate its structure. Results indicated that PPE contained a backbone of (1→2)-linked Man, which was heavily substituted via (1→6)-glucosidic bonds with (1→3)-linked Man, and terminated mainly with Man, as well as a small amount of Gal and Glc. Immunological tests showed that PPE exhibited high macrophages-activating ability, thereby augmenting phagocytosis and enhancing the production of nitric oxide, tumour necrosis factor-α and reactive oxygen species. Therefor, PPE had potential applications in developing functional food or in serving as a natural immnunostimulant.
School of Life Sciences, Northeast Normal University, Changchun 130024,China.
Peng
P
Jiang
Agricultural and Animal Husbandry College of Tibet University, Xizang,860000,China.
Lei
L
Yuan
School of Life Sciences, Northeast Normal University, Changchun 130024,China.
Guohui
G
Huang
School of Life Sciences, Northeast Normal University, Changchun 130024,China.
Xiuli
X
Wang
Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China.
Xia
X
Li
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 130117, China. Electronic address: jianganji@hotmail.com.
Lili
L
Jiao
School of Life Sciences, Northeast Normal University, Changchun 130024,China. Electronic address: zhanglp625@163.com.
Liping
L
Zhang
D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D002240:Carbohydrate Sequence; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice
D000276:Adjuvants, Immunologic; D062610:Fungal Polysaccharides
Macrophage; Phellinus pini; Polysaccharides