Application of optically-induced-dielectrophoresis in microfluidic system for purification of circulating tumour cells for gene expression analysis- Cancer cell line model.
Circulating tumour cells (CTCs) in a blood circulation system are associated with cancer metastasis. The analysis of the drug-resistance gene expression of cancer patients' CTCs holds promise for selecting a more effective therapeutic regimen for an individual patient. However, the current CTC isolation schemes might not be able to harvest CTCs with sufficiently high purity for such applications. To address this issue, this study proposed to integrate the techniques of optically induced dielectrophoretic (ODEP) force-based cell manipulation and fluorescent microscopic imaging in a microfluidic system to further purify CTCs after the conventional CTC isolation methods. In this study, the microfluidic system was developed, and its optimal operating conditions and performance for CTC isolation were evaluated. The results revealed that the presented system was able to isolate CTCs with cell purity as high as 100%, beyond what is possible using the previously existing techniques. In the analysis of CTC gene expression, therefore, this method could exclude the interference of leukocytes in a cell sample and accordingly contribute to higher analytical sensitivity, as demonstrated in this study. Overall, this study has presented an ODEP-based microfluidic system capable of simply and effectively isolating a specific cell species from a cell mixture.
Department of Chemical and Materials Engineering, Chang Gung University, Taoyuan City, 33302, Taiwan (R. O. C.).
Tzu-Keng
TK
Chiu
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan City, 33302, Taiwan (R. O. C.).
Wen-Pin
WP
Chou
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan City, 33302, Taiwan (R. O. C.).
Song-Bin
SB
Huang
Division of Haematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Taoyuan City, 33302, Taiwan (R. O. C.).
Hung-Ming
HM
Wang
Division of Haematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Taoyuan City, 33302, Taiwan (R. O. C.).
Yung-Chang
YC
Lin
Department of Chemical and Materials Engineering, Chang Gung University, Taoyuan City, 33302, Taiwan (R. O. C.).|Division of Haematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Taoyuan City, 33302, Taiwan (R. O. C.).
Chia-Hsun
CH
Hsieh
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan City, 33302, Taiwan (R. O. C.).|Division of Haematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Taoyuan City, 33302, Taiwan (R. O. C.).|Department of Chemical Engineering, Ming Chi University of Technology, Taiwan (R. O. C.).
Min-Hsien
MH
Wu
D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008856:Microscopy, Fluorescence; D009360:Neoplastic Cells, Circulating