Gomesin acts in the immune system and promotes myeloid differentiation and monocyte/macrophage activation in mouse.
Due to the cytotoxic effect of antimicrobial peptides (AMP) against several microorganism and tumor cells has been proposed their association with the immune system. However, just a few reports have shown this relationship. In this study, mice were treated with gomesin, a β-hairpin AMP that exhibit high cytotoxicity against bacterial and tumor cells. Different effects in the immune system were observed, such as, decrease of CD3+in T lymphocytes (Control: 17.7±1.4%; Gomesin: 7.67±1.2%) and in hematopoietic progenitors and increase of hematopoietic stem cell (Control: 0.046±0.004%; Gomesin: 0.067±0.003%), B220+B lymphocytes (Control: 38.63±1.5%; Gomesin: 47.83±0.48%), and Mac-1+F4/80+macrophages (Control: 11.76±3.4%; Gomesin: 27.13±4.0%). Additionally, macrophage increase was accompanied by an increase of macrophage phagocytosis (Control 20.85±1.53; Gomesin 31.32±1 Geometric mean), interleukin 6 (Control: 47.24±1.9ng/mL; Gomesin: 138.68±33.68ng/mL) and monocyte chemoattractant protein-1 (Control: 0.872±0.093ng/mL; Gomesin: 1.83±0.067ng/mL). Thus, this report showed immunomodulatory activity of gomesin in the immune system of mice.
Departamento de Bioquímica, Universidade Federal de São Paulo, R. Três de Maio 100, 04044-020, São Paulo, SP, Brazil.
Marcus V
MV
Buri
Departamento de Bioquímica, Universidade Federal de São Paulo, R. Três de Maio 100, 04044-020, São Paulo, SP, Brazil.
Carol C
CC
Dias
Departamento de Bioquímica, Universidade Federal de São Paulo, R. Três de Maio 100, 04044-020, São Paulo, SP, Brazil.
Christiano M V
CM
Barbosa
Departamento de Biofísica, Universidade Federal de São Paulo, R. Três de Maio 100, 04044-020, São Paulo, SP, Brazil.
Amanda
A
Nogueira-Pedro
Centro Interdisciplinar de Investigação Bioquı́mica, Universidade de Mogi das Cruzes, Av. Dr. Cândido Xavier de Almeida Souza, 200, Mogi das Cruzes, SP, Brazil.
Antonio C
AC
Ribeiro-Filho
Departamento de Biofísica, Universidade Federal de São Paulo, R. Três de Maio 100, 04044-020, São Paulo, SP, Brazil. Electronic address: amiranda@unifesp.br.
Antonio
A
Miranda
Departamento de Bioquímica, Universidade Federal de São Paulo, R. Três de Maio 100, 04044-020, São Paulo, SP, Brazil; Centro Interdisciplinar de Investigação Bioquı́mica, Universidade de Mogi das Cruzes, Av. Dr. Cândido Xavier de Almeida Souza, 200, Mogi das Cruzes, SP, Brazil. Electronic address: paredes.gamero@gmail.com.
Edgar J
EJ
Paredes-Gamero
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000008:administration & dosage / Q000276:immunology*; D002454:Cell Differentiation / Q000235:genetics*; D007107:Immune System / Q000378:metabolism; D056747:Immunomodulation / Q000235:genetics; D008262:Macrophage Activation / Q000235:genetics* / Q000276:immunology; D051379:Mice; D009000:Monocytes / Q000378:metabolism; D022423:Myeloid Cells / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
D023181:Antimicrobial Cationic Peptides; C415987:gomesin
Antimicrobial peptide; Gomesin; Immunomodulatory activity; Macrophage activation