Microbial responses to various process disturbances in a continuous hydrogen reactor fed with galactose.
In this study, microbial responses of a continuous hydrogen reactor fed with galactose have been investigated. Process disturbances reduced H2production performance as well as large fluctuations in microbial diversity. The peak values of the hydrogen yield (HY) was not influenced greatly during the steady state period, and accounted as 2.01 ± 0.05 and 2.14 ± 0.03 mol/mol galactoseadded, while hydraulic retention time (HRT) was at 12 and 8 h, respectively. Microbial community analysis via 454 pyrosequencing revealed that functional redundancy following changes in the microbial community distribution led to the stability of the fermentation performance. The butyrate to acetate (B/A) ratio well correlated with changes in the microbial community. The energy generation rate and energy yield resulted in the peak values of 134 kJ/L-d and 612 kJ/moladded.
Sustainable Management of Natural Resources and Environment Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam; Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea.
Gopalakrishnan
G
Kumar
School of Civil, Environmental and Architectural Engineering, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-714, South Korea.
Jeong-Hoon
JH
Park
Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea.
Periyasamy
P
Sivagurunathan
School of Civil, Environmental and Architectural Engineering, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-714, South Korea; Department of Microbiology & Molecular Genetics, Michigan State University, East Lansing, MI 48824-4320, USA.
Sang-Hoon
SH
Lee
School of Civil, Environmental and Architectural Engineering, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-714, South Korea.
Hee-Deung
HD
Park
Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea. Electronic address: sanghkim1@daegu.ac.kr.
Sang-Hyoun
SH
Kim
D000220:Adaptation, Biological / Q000187:drug effects / Q000235:genetics; D001674:Bioelectric Energy Sources / Q000382:microbiology; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology*; D020148:Butyric Acid / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects / Q000235:genetics; D005690:Galactose / Q000378:metabolism / Q000494:pharmacology*; D006859:Hydrogen / Q000737:chemistry / Q000378:metabolism*; D059013:Microbial Consortia / Q000187:drug effects / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
D056804:Biofuels; D020148:Butyric Acid; D006859:Hydrogen; D005690:Galactose
454 Pyrosequencing; Biohydrogen; Energy generation rate; Functional redundancy; Microbial consortia