Effects of polycyclic aromatic compounds in fine particulate matter generated from household coal combustion on response to EGFR mutations in vitro.
Induction of PM2.5-associated lung cancer in response to EGFR-tyrosine kinase inhibitors (EGFR-TKI) remains unclear. Polycyclic aromatic hydrocarbons (PAHs) and their polar derivatives (oxygenated PAHs: OPAHs and azaarenes: AZAs) were characterized in fine particulates (PM2.5) emitted from indoor coal combustion. Samples were collected in Xuanwei (Yunnan Province), a region in China with a high rate of lung cancer. Human lung adenocarcinoma cells A549 (with wild-type EGFR) and HCC827 (with EGFR mutation) were exposed to the PM2.5, followed by treatment with EGFR-TKI. Two samples showed significant and dose-dependent reduction in the cell viability in A549. EGFR-TKI further demonstrated significantly decreased in cell viability in A549 after exposure to the coal emissions. Chrysene and triphenylene, dibenzo[a,h]anthracene, benzo[ghi]perylene, azaarenes and oxygenated polycyclic aromatic hydrocarbons (carbonyl-OPAHs) were all associated with EGFR-TKI-dependent reduced cell viability after 72-h exposure to the PM2.5. The findings suggest the coal emissions could influence the response of EGFR-TKI in lung cancer cells in Xuanwei.
Jockey Club School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong; Shenzhen Municipal Key Laboratory for Health Risk Analysis, Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China.
Kin-Fai
KF
Ho
Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan; Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Chih-Cheng
CC
Chang
School of Public Health, The University of Hong Kong, Hong Kong.
Linwei
L
Tian
Jockey Club School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong.
Chi-Sing
CS
Chan
Institute of Geography, University of Bern, Hallerstrasse 12, 3012 Bern, Switzerland; Oeschger Centre for Climate Change Research, University of Bern, Falkenplatz 16, 3012 Bern, Switzerland.
Benjamin A
BA
Musa Bandowe
Jockey Club School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong.
Ka-Hei
KH
Lui
Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan; Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Kang-Yun
KY
Lee
Department of Public Health, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan.
Kai-Jen
KJ
Chuang
Department of Thoracic Medicine, Chang Gung Memorial Hospital, Chang Gung University School of Medicine, Taipei, Taiwan; Department of Thoracic Medicine, Division of Oncology and Interventional Bronchoscopy, Chang Gung Memorial Hospital, Taipei, Taiwan.
Chien-Ying
CY
Liu
School of Energy and Environment, City University of Hong Kong, Hong Kong.
Zhi
Z
Ning
Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan; Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan. Electronic address: r92841005@ntu.edu.tw.
Hsiao-Chi
HC
Chuang
D000393:Air Pollutants / Q000737:chemistry / Q000633:toxicity*; D045744:Cell Line, Tumor; D002681:China; D002911:Chrysenes; D003031:Coal / Q000032:analysis*; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D005191:Family Characteristics; D006801:Humans; D009154:Mutation; D052638:Particulate Matter / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D012307:Risk Factors
D000393:Air Pollutants; D002911:Chrysenes; D003031:Coal; D052638:Particulate Matter; D011084:Polycyclic Aromatic Hydrocarbons; C031180:chrysene; C009590:triphenylene; D066246:ErbB Receptors
Coal combustion; Lung cancer; Polycyclic aromatic compounds; Xuanwei