Role of NLRP3 Inflammasome in Eosinophilic and Non-eosinophilic Chronic Rhinosinusitis with Nasal Polyps.
The pathophysiologic mechanisms of human chronic rhinosinusitis with nasal polyps (CRSwNP) remain unclear. We aimed to elucidate expression and biologic role of NLRP3 inflammasome in CRSwNP. Immunohistochemistry (IHC) was conducted to assess NLRP3 immunolabeling, real-time polymerase chain reaction (PCR) was used for IL-9 and NLRP3, and caspase-1 level quantitation in CRSwNP and control subjects. In addition, enzyme-linked immunosorbent assay (ELISA) was employed for analyzing concentrations of IL-1β and IL-18 in the homogenates prepared from tissue specimens. Moreover, human nasal epithelial cells (HNECs) were used to evaluate the effects of lipopolysaccharide (LPS) and glyburide on NLRP3 inflammasome signaling pathway. Results showed that NLRP3 and caspase-1 were overexpressed in CRSwNP, especially in eosinophilic CRSwNP (ECRSwNP). Interestingly, NLRP3 expression had close correlation to that of caspase-1. Concentrations of IL-1β and IL-18 were elevated. NLRP3 inflammasome signaling pathway was augmented by LPS but suppressed by glyburide. In conclusion, NLRP3 inflammasome signaling pathway played a pro-inflammatory role in the pathogenesis of CRSwNP, especially in ECRSwNP. NLRP3 inflammasome signaling pathway was augmented by LPS, but suppressed by glyburide.
Department of Otorhinolaryngology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China. lhi2012@sina.com.|Department of Otorhinolaryngology, Fuzhou General Hospital, Fuzhou, 350025, Fujian, China. lhi2012@sina.com.
Hai
H
Lin
Department of Otorhinolaryngology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
Zhipeng
Z
Li
Department of Biology and Chemical Engineering, Fuqing Branch of Fujian Normal University, Fuqing, 350300, Fujian, China.
Dong
D
Lin
Department of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, Shanghai, 200031, China.
Chunquan
C
Zheng
Department of Otorhinolaryngology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China. drzhangwt@sina.com.
Weitian
W
Zhang
D016022:Case-Control Studies; D002908:Chronic Disease; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005905:Glyburide / Q000494:pharmacology; D006801:Humans; D058847:Inflammasomes / Q000737:chemistry*; D020382:Interleukin-18 / Q000032:analysis / Q000276:immunology; D053583:Interleukin-1beta / Q000032:analysis / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000502:physiology*; D009298:Nasal Polyps; D012220:Rhinitis / Q000276:immunology* / Q000473:pathology; D015398:Signal Transduction / Q000187:drug effects; D012852:Sinusitis / Q000276:immunology* / Q000473:pathology
D058847:Inflammasomes; D020382:Interleukin-18; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D005905:Glyburide
NLRP3; caspase-1; chronic rhinosinusitis; eosinophil; glyburide; nasal polyps