Development of a Consistent and Reproducible Porcine Scald Burn Model.
There are very few porcine burn models that replicate scald injuries similar to those encountered by children. We have developed a robust porcine burn model capable of creating reproducible scald burns for a wide range of burn conditions. The study was conducted with juvenile Large White pigs, creating replicates of burn combinations; 50°C for 1, 2, 5 and 10 minutes and 60°C, 70°C, 80°C and 90°C for 5 seconds. Visual wound examination, biopsies and Laser Doppler Imaging were performed at 1, 24 hours and at 3 and 7 days post-burn. A consistent water temperature was maintained within the scald device for long durations (49.8 ± 0.1°C when set at 50°C). The macroscopic and histologic appearance was consistent between replicates of burn conditions. For 50°C water, 10 minute duration burns showed significantly deeper tissue injury than all shorter durations at 24 hours post-burn (p ≤ 0.0001), with damage seen to increase until day 3 post-burn. For 5 second duration burns, by day 7 post-burn the 80°C and 90°C scalds had damage detected significantly deeper in the tissue than the 70°C scalds (p ≤ 0.001). A reliable and safe model of porcine scald burn injury has been successfully developed. The novel apparatus with continually refreshed water improves consistency of scald creation for long exposure times. This model allows the pathophysiology of scald burn wound creation and progression to be examined.
Centre for Children's Burns and Trauma Research, The University of Queensland, Centre for Children's Health Research, South Brisbane, Queensland, Australia.
Christine J
http://orcid.org/0000-0003-4513-1349
CJ
Andrews
Centre for Children's Burns and Trauma Research, The University of Queensland, Centre for Children's Health Research, South Brisbane, Queensland, Australia.
Margit
M
Kempf
Centre for Children's Burns and Trauma Research, The University of Queensland, Centre for Children's Health Research, South Brisbane, Queensland, Australia.
Roy
R
Kimble
Centre for Children's Burns and Trauma Research, Queensland University of Technology, School of Biomedical Sciences, Institute of Health and Biomedical Innovation at Centre for Children's Health Research, South Brisbane, Queensland, Australia.
Leila
L
Cuttle
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002056:Burns / Q000473:pathology*; D020405:Dermis / Q000473:pathology; D004195:Disease Models, Animal; D004301:Doppler Effect; D005260:Female; D006801:Humans; D021621:Imaging, Three-Dimensional; D007834:Lasers; D015203:Reproducibility of Results; D013194:Staining and Labeling; D034421:Sus scrofa; D013696:Temperature; D014867:Water
D015415:Biomarkers; D014867:Water