MIRU-VNTR allelic variability depends on Mycobacterium bovis clonal group identity.
The description of the population of M. bovis strains circulating in France from 1978 to 2013 has highlighted the discriminating power of the MLVA among predominant spoligotype groups. In the present study we aimed to characterize clonal groups via MLVA and to better understand the strain's population structure. MLVA was performed with eight MIRU-VNTR loci, most of them defined by the Venomyc European consortium. The discriminatory index of each MLVA loci was calculated for SB0120, SB0134, SB0121 and the "F4-family", the main spoligotype groups in France. Differences in global DI per spoligotype, but also by locus within each spoligotype, were observed, which strongly suggest the clonal complex nature of these major groups. These MLVA results were compared to those of other European countries where strain collections had been characterized (Spain, Portugal, Italy, Northern Ireland and Belgium). Overall, QUB 3232 and ETR D are respectively the most and the least discriminative loci, regardless of the strains geographical origin. However, marked DI differences are observed in the rest of the MIRU-VNTR loci, again highlighting that strain genetic variability in a country depends on the dominant existing clonal complexes. A web application for M. bovis, including spoligotyping and MIRU-VNTR typing data, was developed to allow inter-laboratory comparison of field isolates. In conclusion, combination of typing methods is required for M. bovis optimum discrimination and differentiation of groups of strains. Thus, the loci employed for MLVA in a country should be those which are the most discriminative for the clonal complexes which characterize their M. bovis population.
Université Paris-Est, Laboratoire National de Référence Tuberculose, Unité Zoonoses Bactériennes, Laboratoire de Santé Animale, ANSES, 94706 Maisons-Alfort Cedex, France; INRA, Université de Tours, UMR1282, Infectiologie et Santé Publique, F-37380 Nouzilly, France.
Amandine
A
Hauer
Université Paris-Est, Laboratoire National de Référence Tuberculose, Unité Zoonoses Bactériennes, Laboratoire de Santé Animale, ANSES, 94706 Maisons-Alfort Cedex, France.
Lorraine
L
Michelet
Université Paris-Est, Laboratoire National de Référence Tuberculose, Unité Zoonoses Bactériennes, Laboratoire de Santé Animale, ANSES, 94706 Maisons-Alfort Cedex, France.
Krystel
K
De Cruz
INRA, Université de Tours, UMR1282, Infectiologie et Santé Publique, F-37380 Nouzilly, France.
Thierry
T
Cochard
INRA, Université de Tours, UMR1282, Infectiologie et Santé Publique, F-37380 Nouzilly, France.
Maxime
M
Branger
Université Paris-Est, Laboratoire National de Référence Tuberculose, Unité Zoonoses Bactériennes, Laboratoire de Santé Animale, ANSES, 94706 Maisons-Alfort Cedex, France.
Claudine
C
Karoui
Université Paris-Est, Laboratoire National de Référence Tuberculose, Unité Zoonoses Bactériennes, Laboratoire de Santé Animale, ANSES, 94706 Maisons-Alfort Cedex, France.
Sylvie
S
Henault
INRA, Université de Tours, UMR1282, Infectiologie et Santé Publique, F-37380 Nouzilly, France.
Franck
F
Biet
Université Paris-Est, Laboratoire National de Référence Tuberculose, Unité Zoonoses Bactériennes, Laboratoire de Santé Animale, ANSES, 94706 Maisons-Alfort Cedex, France. Electronic address: maria-laura.boschiroli@anses.fr.
María Laura
ML
Boschiroli
D000818:Animals; D002417:Cattle; D005060:Europe / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D008322:Mammals / Q000382:microbiology; D018598:Minisatellite Repeats / Q000235:genetics*; D058889:Molecular Typing / Q000379:methods*; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics*; D014380:Tuberculosis, Bovine / Q000382:microbiology*