Divergent evolution of multiple virus-resistance genes from a progenitor in Capsicum spp.
Plants have evolved hundreds of nucleotide-binding and leucine-rich domain proteins (NLRs) as potential intracellular immune receptors, but the evolutionary mechanism leading to the ability to recognize specific pathogen effectors is elusive. Here, we cloned Pvr4 (a Potyvirus resistance gene in Capsicum annuum) and Tsw (a Tomato spotted wilt virus resistance gene in Capsicum chinense) via a genome-based approach using independent segregating populations. The genes both encode typical NLRs and are located at the same locus on pepper chromosome 10. Despite the fact that these two genes recognize completely different viral effectors, the genomic structures and coding sequences of the two genes are strikingly similar. Phylogenetic studies revealed that these two immune receptors diverged from a progenitor gene of a common ancestor. Our results suggest that sequence variations caused by gene duplication and neofunctionalization may underlie the evolution of the ability to specifically recognize different effectors. These findings thereby provide insight into the divergent evolution of plant immune receptors.
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
Saet-Byul
SB
Kim
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.|Department of Horticulture, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, 660-701, Korea.
Won-Hee
WH
Kang
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
Hoang Ngoc
HN
Huy
Department of Horticulture, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, 660-701, Korea.
Seon-In
SI
Yeom
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
Jeong-Tak
JT
An
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
Seungill
S
Kim
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
Min-Young
MY
Kang
Department of Eco-Friendly Horticulture, Cheonan Yonam College, Cheonan, 331-709, Korea.
Hyun Jung
HJ
Kim
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.|Korea Atomic Energy Research Institute, Jeongeup, 580-185, Korea.
Yeong Deuk
YD
Jo
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
Yeaseong
Y
Ha
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
Doil
D
Choi
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea.
Byoung-Cheorl
BC
Kang
D002212:Capsicum / Q000235:genetics* / Q000821:virology*; D020090:Chromosome Segregation / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D019143:Evolution, Molecular; D017343:Genes, Plant; D056426:Genetic Loci; D005810:Multigene Family; D020161:Physical Chromosome Mapping; D010935:Plant Diseases / Q000821:virology*; D030821:Plants, Genetically Modified; D017800:Potyvirus / Q000502:physiology*; D014026:Nicotiana / Q000821:virology
Pvr4; Tsw; evolution; hot pepper; plant disease; plant virus resistance