Effects of chlorogenic acid on voltage-gated potassium channels of trigeminal ganglion neurons in an inflammatory environment.
Chlorogenic acid (CGA) composed of coffee acid and quinic acid is an effective ingredient of many foods and medicines and widely exhibits biological effects. Recently, it is reported to have analgesic effect. However, little is known about the analgesic mechanism of CGA. In this study, whole-cell patch-clamp recordings were performed on two main subtypes (IK,Aand IK,Vchannels) of voltage-gated potassium (KV) channels in small-diameter(<30μm) trigemianl ganglion neurons to analyze the effects of CGA in an inflammatory environment created by Prostaglandin E2(PGE2). On one hand, the activation and inactivation V1/2values of IK,Aand IK,Vchannels showed an elevation towards a depolarizing shift caused by PGE2. On the other hand, the activation and inactivation V1/2values of the two channels had a reduction towards a hyperpolarizing shift caused by CGA under PGE2pretreatment. Our results demonstrated that CGA may exhibited an analgesic effect by promoting KVchannels activation and inactivation under inflammatory condition, which provided a novel molecular and ionic mechanism underlying anti-inflammatory pain of CGA.
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province 610041, China.
Fei
F
Liu
Ningbo Dental Hospital, Ningbo, Zhejiang Province 315000, China.
Xiao-Wen
XW
Lu
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province 610041, China.
Yu-Jiao
YJ
Zhang
Ningbo Dental Hospital, Ningbo, Zhejiang Province 315000, China.
Liang
L
Kou
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province 610041, China.
Ning
N
Song
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province 610041, China.
Min-Ke
MK
Wu
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province 610041, China.
Min
M
Wang
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province 610041, China.
Hang
H
Wang
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province 610041, China. Electronic address: shenjiefei@scu.edu.cn.
Jie-Fei
JF
Shen
D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000494:pharmacology*; D015232:Dinoprostone; D004195:Disease Models, Animal; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000276:immunology; D010146:Pain / Q000188:drug therapy / Q000378:metabolism; D018408:Patch-Clamp Techniques; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D012668:Trigeminal Ganglion / Q000187:drug effects* / Q000276:immunology
D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D024642:Potassium Channels, Voltage-Gated; D002726:Chlorogenic Acid; D015232:Dinoprostone
Chlorogenic acid; Patch clamp; Prostaglandin E(2); Trigeminal ganglion neurons; Voltage-gated potassium channels