Protective role of selenium in the activities of antioxidant enzymes in piglet splenic lymphocytes exposed to deoxynivalenol.
We evaluated the effects of selenium (Se) on antioxidant enzymes of piglet splenic lymphocytes exposed to deoxynivalenol (DON). We measured cell viability, the activities of several antioxidant enzymes, and lactate dehydrogenase (LDH), as well as total antioxidant capacity (T-AOC) and the levels of malonaldehyde (MDA) and hydrogen peroxide (H2O2). We found that DON exposure increased the concentrations of LDH, MDA, and H2O2in all experimental groups in a dose-dependent manner, while the concentrations of other antioxidant enzymes were decreased. In Se-pretreated DON-exposed cells, damage to antioxidant enzymes was reduced, especially in the lower-dose DON groups over longer exposure times. These results may indicate that in piglet splenic lymphocytes, Se can alleviate DON-induced damage to antioxidant enzymes by improving glutathione peroxidase activity. Se may function as a potential antioxidative agent to alleviate DON-induced oxidative stress.
College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: 2319479354@qq.com.
Xuemei
X
Wang
College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: zzcjl@126.com.
Zhicai
Z
Zuo
College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: 330546139@qq.com.
Chuanping
C
Zhao
College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: 631335814@qq.com.
Zhuo
Z
Zhang
College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: Pgn.sicau@163.com.
Guangneng
G
Peng
College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: suizhongcao@126.com.
Suizhong
S
Cao
College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: hychun114@163.com.
Yanchun
Y
Hu
College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: yayushumin@163.com.
Shumin
S
Yu
College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: zhongzhijun488@126.com.
Zhijun
Z
Zhong
College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: dengjl213@126.com.
Junliang
J
Deng
College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu 611130, China. Electronic address: zhihua_ren@126.com.
Zhihua
Z
Ren
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology; D012643:Selenium / Q000494:pharmacology*; D018038:Sodium Selenite / Q000494:pharmacology; D013154:Spleen / Q000166:cytology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013552:Swine; D014255:Trichothecenes / Q000633:toxicity*
D000975:Antioxidants; D020011:Protective Agents; D014255:Trichothecenes; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D012643:Selenium; D018038:Sodium Selenite; C007262:deoxynivalenol
Antioxidant enzymes; Deoxynivalenol; Selenium; Splenic lymphocytes