Reagentless colorimetric biosensing platform based on nanoceria within an agarose gel matrix.
A composite consisting of cerium oxide nanoparticles (nanoceria) and an oxidative enzyme co-entrapped in an agarose gel has been developed for the reagent-free colorimetric detection of biologically important target molecules. The oxidase, immobilized in the agarose matrix, promotes the oxidation of target molecules to generate H2O2that subsequently induces changes in the physicochemical properties of nanoceria exhibiting a color change from white/light-yellow into intense-yellow/light-orange without any requirement for additional colorimetric substrates. By utilizing the unique color-changing property of nanoceria entrapped within the agarose gel, target glucose molecules were very specifically detected over a wide linear range from 0.05 to 2mM, which is suitable to measure the serum glucose level, with excellent operational stability over two weeks at room temperature. The biosensor also exhibited a high degree of precision and reproducibility when employed to detect glucose present in real human serum samples. We expect that this novel nanoceria-based biosensing format could be readily extended to other oxidative enzymes for the convenient detection of various clinically important target molecules.
Department of BioNano Technology, Gachon University, Gyeonggi 461-701, Republic of Korea.
Do Hyun
DH
Kim
Department of Chemical and Biological Engineering, Gachon University, Gyeonggi 461-701, Republic of Korea.
Jaehyun
J
Hur
Department of Chemical and Biomolecular Engineering (BK21+ Program), Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea. Electronic address: hgpark@kaist.ac.kr.
Hyun Gyu
HG
Park
Department of BioNano Technology, Gachon University, Gyeonggi 461-701, Republic of Korea. Electronic address: moonil@gachon.ac.kr.
Moon
M
Il Kim
D015374:Biosensing Techniques / Q000379:methods*; D002563:Cerium / Q000737:chemistry; D003124:Colorimetry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry*
D004800:Enzymes, Immobilized; D002563:Cerium; C030583:ceric oxide; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D005947:Glucose
Agarose gel; Colorimetric biosensor; Glucose detection; Hydrogen peroxide detection; Nanoceria