Anti-wrinkle and anti-whitening effects of jucá (Libidibia ferrea Mart.) extracts.
Skin aging is a natural process of the human body that may be accelerated due to extrinsic causes. Libidibia ferrea, popularly known as jucá, is a small tree, which possesses an abundant phenolic composition with potential antioxidant and enzymatic inhibition activities. Thus, this work aimed to investigate the anti-wrinkle and anti-whitening potentials of jucá trunk bark (LFB) and pod (LFP) extracts. A comprehensive analysis of LFB and LFP phenolic composition was accomplished by means of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Effects on skin degradation were assessed by inhibitory enzymatic activity against elastase, hyaluronidase and collagenase through colorimetric assays. Cellular viability in B16F10 and primary fibroblasts were determined by Trypan Blue exclusion assay. Anti-melanogenic effects on B16F10 cells were evaluated using cellular tyrosinase, melanin content, western blot, and RT-qPCR analyses. Inhibition of matrix metalloproteinase-2 and metalloproteinase-9 (MMP-2 and MMP-9) was determined by gelatin zymography and western blot methodologies. LC-MS/MS analyses of LFB and LFP extracts allowed the characterization of 18 compounds, among them, flavonoids, phenolic acids, and secoridoids. Additionally the pod and trunk bark compositions were compared. Hyaluronidase inhibitory activity for both extracts, LFB (IC50= 8.5 ± 0.8 µg/mL) and LFP (IC50= 16 ± 0.5 µg/mL), was stronger than standard rutin (IC50= 27.6 ± 0.06). Pro-MMP-2 was significantly inhibited by both extracts. LFB and LFP decreased the melanin content in B16F10 due to tyrosinase inhibitory activity. L. ferrea extracts has high potential as a cosmetic ingredient due to its anti-wrinkle and depigmentant effects.
Faculty of Pharmaceutical Sciences, Federal University of Amazonas (UFAM), General Rodrigo Otávio Campos de Jordão Avenue, 6200, Coroado, Manaus, AM, CEP: 69077-000, Brazil.|School of Pharmaceutical Sciences, University of São Paulo (USP), São Paulo, Brazil.
Tatiana do Nascimento
TD
Pedrosa
Faculty of Pharmaceutical Sciences, Federal University of Amazonas (UFAM), General Rodrigo Otávio Campos de Jordão Avenue, 6200, Coroado, Manaus, AM, CEP: 69077-000, Brazil.
Aline Oliveira
AO
Barros
Faculty of Pharmaceutical Sciences, Federal University of Amazonas (UFAM), General Rodrigo Otávio Campos de Jordão Avenue, 6200, Coroado, Manaus, AM, CEP: 69077-000, Brazil.
Jéssica Rodrigues
JR
Nogueira
School of Pharmaceutical Sciences, University of São Paulo (USP), São Paulo, Brazil.
Andréa Costa
AC
Fruet
Faculty of Pharmaceutical Sciences, Federal University of Amazonas (UFAM), General Rodrigo Otávio Campos de Jordão Avenue, 6200, Coroado, Manaus, AM, CEP: 69077-000, Brazil.
Isis Costa
IC
Rodrigues
Research Group in Oncology, Federal University of Pará (UFPA), Belém, Brazil.|Department of Morphology and Genetic, Federal University of São Paulo, São Paulo, Brazil.
Danielle Queiroz
DQ
Calcagno
Department of Morphology and Genetic, Federal University of São Paulo, São Paulo, Brazil.
Marilia de Arruda Cardoso
MA
Smith
Faculty of Pharmaceutical Sciences, Federal University of Amazonas (UFAM), General Rodrigo Otávio Campos de Jordão Avenue, 6200, Coroado, Manaus, AM, CEP: 69077-000, Brazil.
Tatiane Pereira
TP
de Souza
School of Pharmaceutical Sciences, University of São Paulo (USP), São Paulo, Brazil.
Silvia Berlanga de Moraes
SB
Barros
Faculty of Pharmaceutical Sciences, Federal University of Amazonas (UFAM), General Rodrigo Otávio Campos de Jordão Avenue, 6200, Coroado, Manaus, AM, CEP: 69077-000, Brazil.
Marne Carvalho
MC
de Vasconcellos
Dempster Mass Spectrometry Group, Amazonas State University (UEA), Manaus, Brazil.
Felipe Moura Araújo da
FM
Silva
Dempster Mass Spectrometry Group, Amazonas State University (UEA), Manaus, Brazil.
Hector Henrique Ferreira
HH
Koolen
School of Pharmaceutical Sciences, University of São Paulo (USP), São Paulo, Brazil.
Silvya Stuchi
SS
Maria-Engler
Faculty of Pharmaceutical Sciences, Federal University of Amazonas (UFAM), General Rodrigo Otávio Campos de Jordão Avenue, 6200, Coroado, Manaus, AM, CEP: 69077-000, Brazil. eslima@ufam.edu.br.
Emerson Silva
ES
Lima
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D029911:Caesalpinia / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival; D003358:Cosmetics / Q000494:pharmacology; D004792:Enzyme Precursors / Q000378:metabolism; D005347:Fibroblasts; D005419:Flavonoids / Q000494:pharmacology; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008543:Melanins / Q000378:metabolism*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology*; D061251:Primary Cell Culture; D015595:Skin Aging / Q000187:drug effects*; D053719:Tandem Mass Spectrometry
D000975:Antioxidants; D003358:Cosmetics; D004792:Enzyme Precursors; D005419:Flavonoids; D008543:Melanins; D010636:Phenols; D010936:Plant Extracts; D014442:Monophenol Monooxygenase; D018093:Gelatinases; C069860:progelatinase; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
Libidibia ferrea; Melanogenesis; Polyphenols; Skin aging; Tyrosinase