Highly efficient recovery of xylobiose from xylooligosaccharides using a simulated moving bed method.
Xylobiose (X2), which is currently available from xylooligosaccharides (XOS), has been reported to have outstanding prebiotic function and to be highly suitable for application in food industries. This has sparked an interest in the economical production of X2 of high purity (> 99%) in food and prebiotic industries. To address such issue, we developed a highly-efficient chromatographic method for the recovery of X2 from XOS with high purity and high recovery. As a first step for this work, an eligible adsorbent for a large-scale separation between X2 and other XOS components was selected. For the selected adsorbent, a single-column experiment was carried out to determine the intrinsic parameters of all the XOS components, which were then used in the optimal design of the continuous X2-recovery process based on a simulated moving bed (SMB) chromatographic method. Finally, the performance of the designed X2-recovery SMB process was verified by the relevant SMB experiments, which confirmed that the developed process in this study could recover X2 from XOS with the purity of 99.5% and the recovery of 92.3% on a continuous-separation mode. The results of this study will be useful in enabling the economical production of high-purity X2 on a large scale.
Department of Chemical Engineering, Hanyang University, Haengdang-dong, Seongdong-gu, Seoul, 04763, South Korea.
Jae-Hwan
JH
Choi
Department of Chemical Engineering, Hanyang University, Haengdang-dong, Seongdong-gu, Seoul, 04763, South Korea.
Hangil
H
Park
Department of Chemical Engineering, Hanyang University, Haengdang-dong, Seongdong-gu, Seoul, 04763, South Korea.
Chanhun
C
Park
School of Chemical Engineering, 480 Stadium Mall Drive, Purdue University, West Lafayette, IN 47907-2100, USA.
Nien-Hwa Linda
NH
Wang
Department of Chemical Engineering, Hanyang University, Haengdang-dong, Seongdong-gu, Seoul, 04763, South Korea. Electronic address: munsy@hanyang.ac.kr.
Sungyong
S
Mun
D000327:Adsorption; D001089:Arabinose / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification*; D005947:Glucose / Q000737:chemistry; D005965:Glucuronates / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D014312:Trisaccharides / Q000737:chemistry; D014994:Xylose / Q000737:chemistry
D004187:Disaccharides; D005965:Glucuronates; D009844:Oligosaccharides; D014312:Trisaccharides; C570991:xylooligosaccharide; C515044:xylotriose; D014994:Xylose; D001089:Arabinose; C004173:xylobiose; D005947:Glucose
High purity; Prebiotics; Simulated moving bed; Xylobiose; Xylooligosaccharides