The effect of H19-miR-29b interaction on bleomycin-induced mouse model of idiopathic pulmonary fibrosis.
Pulmonary fibrosis, characterized by the destruction of lung tissue architecture and the formation of fibrous foci, currently has no satisfactory treatment. Emodin is a component of Chinese herb that has been reported to be medicable on pancreatic fibrosis and liver fibrosis. However, its role in pulmonary fibrosis has not been reported yet. In the present study, we investigated the hypothesis that H19 play a promotive role in bleomycin-induced epithelial-mesenchymal transition of alveolar epithelial cell, and H19 exerts its effect through miR-29b regulation. H19 expression was positively correlated with COL1A1 and Acta2 expression; H19 knockdown inhibited COL1A1 and Acta2 expression. Moreover, H19 interacted with miR-29b through directly binding to the 3'UTR; miR-29b inhibited COL1A1 expression by directly binding to the 3'UTR. In conclusion, we revealed the promotive effect of H19 on BLM-induced IPF, and demonstrated the mechanism by which H19/miR-29b interaction exerts its effect on regulating pulmonary fibrosis. The present study provided a potential therapy to treat IPF.
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, Key Institute of National Clinical Research Center for Respiratory Disease of China, China. Electronic address: tyj7736@163.com.
Yongjun
Y
Tang
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, Key Institute of National Clinical Research Center for Respiratory Disease of China, China.
Ruoxi
R
He
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, Key Institute of National Clinical Research Center for Respiratory Disease of China, China.
Jian
J
An
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, Key Institute of National Clinical Research Center for Respiratory Disease of China, China.
Pengbo
P
Deng
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, Key Institute of National Clinical Research Center for Respiratory Disease of China, China.
Li
L
Huang
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, Key Institute of National Clinical Research Center for Respiratory Disease of China, China.
Wei
W
Yang
D020413:3' Untranslated Regions; D000199:Actins / Q000378:metabolism; D000818:Animals; D001761:Bleomycin / Q000737:chemistry*; D049109:Cell Proliferation; D024042:Collagen Type I / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D005786:Gene Expression Regulation; D054990:Idiopathic Pulmonary Fibrosis / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics*; D041681:NIH 3T3 Cells; D010179:Pancreas / Q000503:physiopathology; D028321:Plant Preparations / Q000737:chemistry; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
D020413:3' Untranslated Regions; C558396:Acta2 protein, mouse; D000199:Actins; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; C094335:H19 long non-coding RNA; C547014:MIRN29 microRNA, mouse; D035683:MicroRNAs; D028321:Plant Preparations; D062085:RNA, Long Noncoding; D001761:Bleomycin
Acta2; Bleomycin; COL1A1; H19; Idiopathic pulmonary fibrosis; miR-29b