Evaluation of DNA binding, DNA cleavage, protein binding, radical scavenging and in vitro cytotoxic activities of ruthenium(II) complexes containing 2,4-dihydroxy benzylidene ligands.
The new ruthenium(II) complexes with hydrazone ligands, 4-Methyl-benzoic acid (2,4-dihydroxy-benzylidene)-hydrazide (HL(1)), 4-Methoxy-benzoic acid (2,4-dihydroxy-benzylidene)-hydrazide (HL(2)), 4-Bromo-benzoic acid (2,4-dihydroxy-benzylidene)-hydrazide (HL(3)), were synthesized and characterized by various spectro analytical techniques. The molecular structures of the ligands were confirmed by single crystal X-ray diffraction technique. The DNA binding studies of the ligands and complexes were examined by absorption, fluorescence, viscosity and cyclic voltammetry methods. The results indicated that the ligands and complexes could interact with calf thymus DNA (CT-DNA) through intercalation. The DNA cleavage activity of the complexes was evaluated by gel electrophoresis assay, which revealed that the complexes are good DNA cleaving agents. The binding interaction of the ligands and complexes with bovine serum albumin (BSA) was investigated using fluorescence spectroscopic method. Antioxidant studies showed that the complexes have a strong radical scavenging properties. Further, the cytotoxic effect of the complexes examined on cancerous cell lines showed that the complexes exhibit significant anticancer activity.
Postgraduate and Research Department of Chemistry, Sri Ramakrishna Mission Vidyalaya College of Arts and Science, Coimbatore-641 020, Tamil Nadu, India.
Maruthachalam
M
Mohanraj
Postgraduate and Research Department of Chemistry, Sri Ramakrishna Mission Vidyalaya College of Arts and Science, Coimbatore-641 020, Tamil Nadu, India.
Ganesan
G
Ayyannan
Postgraduate and Research Department of Chemistry, Sri Ramakrishna Mission Vidyalaya College of Arts and Science, Coimbatore-641 020, Tamil Nadu, India.
Gunasekaran
G
Raja
Postgraduate and Research Department of Chemistry, Sri Ramakrishna Mission Vidyalaya College of Arts and Science, Coimbatore-641 020, Tamil Nadu, India. Electronic address: drcjbstar@gmail.com.
Chinnasamy
C
Jayabalakrishnan
D000970:Antineoplastic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001665:Binding Sites; D001667:Binding, Competitive / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects; D004583:Electrons; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D006835:Hydrazones / Q000138:chemical synthesis / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008024:Ligands; D061986:MCF-7 Cells; D013058:Mass Spectrometry; D011485:Protein Binding / Q000187:drug effects; D066244:Proton Magnetic Resonance Spectroscopy; D012118:Resorcinols / Q000737:chemistry*; D012428:Ruthenium / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity
D000970:Antineoplastic Agents; D000975:Antioxidants; D056831:Coordination Complexes; D016166:Free Radical Scavengers; D006835:Hydrazones; D008024:Ligands; D012118:Resorcinols; D012428:Ruthenium; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium
Antioxidant; Crystal structure; Cytotoxicity; DNA interaction; Ruthenium(II) hydrazone complexes