5-Lipoxygenase inhibitor zileuton inhibits Ca(2+)-responses induced by glutoxim and molixan in macrophages.
Using Fura-2AM microfluorimetry, we have shown for the first time that 5-lipoxygenase specific inhibitor antiasthmatic agent zileuton significantly inhibits Ca(2+)-responses induced by glutoxim and molixan in macrophages. The results support 5-lipoxygenase involvement in the effect of glutoxim and molixan on intracellular Ca(2+) concentration in macrophages and indicate the inadvisability of a combined use of drugs glutoxim and molixan and antiasthmatic agent zileuton.
St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia. z.krutetskaya@spbu.ru.
Z I
ZI
Krutetskaya
St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
L S
LS
Milenina
St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
A A
AA
Naumova
St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
V G
VG
Antonov
St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
A D
AD
Nozdrachev
D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D002413:Cations, Divalent / Q000378:metabolism; D002478:Cells, Cultured; D003592:Cytophotometry; D004338:Drug Combinations; D004347:Drug Interactions; D016257:Fura-2 / Q000031:analogs & derivatives; D006918:Hydroxyurea / Q000031:analogs & derivatives* / Q000494:pharmacology; D007288:Inosine / Q000494:pharmacology*; D042541:Intracellular Space / Q000187:drug effects / Q000201:enzymology; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000201:enzymology; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000494:pharmacology*; D017208:Rats, Wistar
D018927:Anti-Asthmatic Agents; D002413:Cations, Divalent; D004338:Drug Combinations; D016859:Lipoxygenase Inhibitors; D009842:Oligopeptides; C462699:glutoxim; C000611436:inosine glycyl-cysteinyl-glutamate disodium; C049925:fura-2-am; D007288:Inosine; D001094:Arachidonate 5-Lipoxygenase; D002118:Calcium; D016257:Fura-2; C063449:zileuton; D006918:Hydroxyurea