A Ratiometric Fluorescent Probe for Imaging of the Activity of Methionine Sulfoxide Reductase A in Cells.
Methionine sulfoxide reductase A (MsrA) is an enzyme involved in redox balance and signaling, and its aberrant activity is implicated in a number of diseases (for example, Alzheimer's disease and cancer). Since there is no simple small molecule tool to monitor MsrA activity in real time in vivo, we aimed at developing one. We have designed a BODIPY-based probe called (S)-Sulfox-1, which is equipped with a reactive sulfoxide moiety. Upon reduction with a model MsrA (E. coli), it exhibits a bathochromic shift in the fluorescence maximum. This feature was utilized for the real-time ratiometric fluorescent imaging of MsrA activity in E. coli cells. Significantly, our probe is capable of capturing natural variations of the enzyme activity in vivo.
Department of Organic Chemistry, Faculty of Science, Charles University in Prague, Hlavova 2030/8, 12843, Prague 2, Czech Republic.
Nikolai
N
Makukhin
Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 2030/8, 12843, Prague 2, Czech Republic.
Vyacheslav
V
Tretyachenko
Department of Pharmacology and Toxicology, School of Pharmacy, University of Kansas, Lawrence, KS, 66045, USA.
Jackob
J
Moskovitz
Department of Organic Chemistry, Faculty of Science, Charles University in Prague, Hlavova 2030/8, 12843, Prague 2, Czech Republic. misek@natur.cuni.cz.
Jiří
J
Míšek
D004926:Escherichia coli / Q000166:cytology / Q000201:enzymology*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D051150:Methionine Sulfoxide Reductases / Q000032:analysis* / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D061848:Optical Imaging
D005456:Fluorescent Dyes; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase
chirality; fluorescent probes; oxidoreductases; photophysics; redox chemistry