Solid-phase extraction of betanin and isobetanin from beetroot extracts using a dipicolinic acid molecularly imprinted polymer.
Betanin is a natural pigment with significant antioxidant and biological activities currently used as food colorant. The isolation of betanin is problematic due to its instability. In this work, we developed a fast and economic procedure based on molecularly imprinted solid-phase extraction (MISPE) for the selective clean-up of betanin and its stereoisomer isobetanin from beetroot extracts. Dipicolinic acid was used as template for the MIP preparation because of its structural similarity with the chromophore group of betanin. The MISPE procedures were fully optimized allowing the almost complete removal of matrix components such as sugars and proteins, resulting in high extraction recovery of betanin/isobetanin in a single step. Moreover, the whole extraction procedure was performed in environmentally friendly solvents with either ethanol or water. Our MISPE method is very promising for the future development of well-formulated beetroot extract with specified betanin/isobetanin content, ready for food or medicinal use.
Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.
Sofia
S
Nestora
Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.
Franck
F
Merlier
Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.
Elise
E
Prost
Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.
Karsten
K
Haupt
Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France. Electronic address: claire.rossi@utc.fr.
Claire
C
Rossi
Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France. Electronic address: jeanne.tse-sum-bui@utc.fr.
Bernadette
B
Tse Sum Bui
D050859:Betacyanins / Q000302:isolation & purification*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D010848:Picolinic Acids / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D014675:Vegetables / Q000737:chemistry; D014867:Water / Q000737:chemistry
D050859:Betacyanins; D010848:Picolinic Acids; D011108:Polymers; D012997:Solvents; D014867:Water; C020228:betanin; C004860:dipicolinic acid
Antioxidants; Beetroot extract; Betanin; Dipicolinic acid; Molecularly imprinted polymer; Solid phase extraction