Whole genome SNP scanning of snow sheep (Ovis nivicola).
This is the first report performing the whole genome SNP scanning of snow sheep (Ovis nivicola). Samples of snow sheep (n = 18) collected in six different regions of the Republic of Sakha (Yakutia) from 64° to 71° N. For SNP genotyping, we applied Ovine 50K SNP BeadChip (Illumina, United States), designed for domestic sheep. The total number of genotyped SNPs (call rate 90%) was 47796 (88.1% of total SNPs), wherein 1006 SNPs were polymorphic (2.1%). Principal component analysis (PCA) showed the clear differentiation within the species O. nivicola: studied individuals were distributed among five distinct arrays corresponding to the geographical locations of sampling points. Our results demonstrate that the DNA chip designed for domestic sheep can be successfully used to study the allele pool and the genetic structure of snow sheep populations.
Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia. horarka@yandex.ru.
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Deniskova
Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia.|Institute of Biological Problems of Cryolithozone, Siberian Branch, Russian Academy of Sciences, ul. Lenina 41, Yakutsk, 677980, Russia.
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Okhlopkov
Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia.
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Sermyagin
Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia.
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Gladyr'
Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia.
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Bagirov
Division of Livestock Sciences, University of Natural Resources and Life Sciences, Augasse 2-6, Vienna, A-1090, Austria.
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Sölkner
Institute of Biological Problems of Cryolithozone, Siberian Branch, Russian Academy of Sciences, ul. Lenina 41, Yakutsk, 677980, Russia.
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Mamaev
Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia.|Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Veterinärplatz, Vienna, A-1210, Austria.
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Brem
Biotechnology and Molecular Diagnostics Center, Ernst All-Russia Institute of Animal Husbandry, pos. Dubrovitsy, Podol'sk district, Moscow oblast, 142132, Russia.
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Zinov'eva
D000818:Animals; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D058974:Phylogeography; D020641:Polymorphism, Single Nucleotide; D012426:Russia; D012756:Sheep / Q000235:genetics*; D013045:Species Specificity