Molecular approach for detecting early prepatent Schistosoma mansoni infection in Biomphalaria alexandrina snail host.
The present study aimed to evaluate a polymerase chain reaction (PCR) assay used for detection of Schistosoma mansoni infection in Biomphalaria alexandrina snails in early prepatent period and to compare between it and the ordinary detection methods (shedding and crushing). Biomphalaria alexandrina snails are best known for their role as intermediate hosts of S. mansoni. DNA was extracted from infected snails in addition to non-infected "negative control" (to optimized the efficiency of PCR reaction) and subjected to PCR using primers specific to a partial sequence of S. mansoni fructose-1,6-bus phosphate aldolase (SMALDO). SMALDO gene was detected in the infected laboratory snails with 70, 85, and 100 % positivity at the 1st, 3rd, and 7th day of infection, respectively. In contrast, the ordinary method was not sensitive enough in detection of early prepatent infection even after 7 days of infection which showed only 25 % positivity. By comparing the sensitivity of the three methods, it was found that the average sensitivity of shedding method compared to PCR was 23.8 % and the average sensitivity of crushing method compared to PCR was 46.4 % while the sensitivity of PCR was 100 %. We conclude that PCR is superior to the conventional methods and can detect positive cases that were negative when examined by shedding or crushing methods. This can help in detection of the areas and times of high transmission which in turn will be very beneficial in planning of the exact timing of the proper control strategy.
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Adel
A
Farghaly
Department of Animal Wealth Development, Genetics & Genetic Engineering, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Ayman A
AA
Saleh
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Soad
S
Mahdy
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Dalia
D
Abd El-Khalik
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Naglaa F
NF
Abd El-Aal
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Sara A
SA
Abdel-Rahman
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Marwa A
MA
Salama
PCR; Prepatent infection; S. mansoni; SMALDO