Ultrasensitive amperometric immunosensor for PSA detection based on Cu2O@CeO2-Au nanocomposites as integrated triple signal amplification strategy.
In this work, a novel label-free electrochemical immunosensor was developed for the quantitative detection of prostate specific antigen (PSA). To this end, the amino functionalized cuprous oxide @ ceric dioxide (Cu2O@CeO2-NH2) core-shell nanocomposites were prepared to bond gold nanoparticles (Au NPs) by constructing stable Au-N bond between Au NPs and -NH2. Because the synergetic effect presents in Cu2O@CeO2core-shell loaded with Au NPs (Cu2O@CeO2-Au), it shows better electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2) than single Cu2O, Au NPs and Cu2O@CeO2. Featured by large specific surface area, good biocompatibility and good electrochemical properties which can greatly improve the electronic transmission rate, Cu2O@CeO2-Au was used as transducing materials to achieve efficiently capture antibodies and triple signal amplification of the proposed immunosensor. Under the optimal conditions, the proposed immunosensor exhibited a wide linear range from 0.1pg/mL to 100ng/mL with a low detection limit of 0.03pg/mL (S/N=3). Furthermore, the proposed label-free immunosensor has been used to determine PSA in human serum with satisfactory results. Meanwhile, it displayed good reproducibility, acceptable selectivity, and long-term stability, which had promising application in bioassay analysis.
School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Faying
F
Li
School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China; Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: liyueyun71@163.com.
Yueyun
Y
Li
School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Jinhui
J
Feng
School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Yunhui
Y
Dong
School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Ping
P
Wang
School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Lei
L
Chen
School of Life Sciences, Shandong University of Technology, Zibo 255049, PR China.
Zhiwei
Z
Chen
School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Hui
H
Liu
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Qin
Q
Wei
D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D002563:Cerium / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D017430:Prostate-Specific Antigen / Q000097:blood*; D015203:Reproducibility of Results
D055910:Antibodies, Immobilized; D002563:Cerium; C030583:ceric oxide; D006046:Gold; D003300:Copper; D017430:Prostate-Specific Antigen; C030973:cupric oxide
Cu(2)O@CeO(2) core-shell; Gold nanoparticles; Label-free immunosensor; Prostate specific antigen