Genotoxicity assessment of low-level doses of gamma radiation with the SOS chromotest and the Ames test.
This is the first study to present data on the genotoxicity of low γ-irradiation doses for E. coli and S. typhimurium cells obtained using the SOS chromotest and the Ames test. The most pronounced effect was recorded in the first 24 h of γ-irradiation. After 72 h in the Ames test and after 96 h in the SOS chromotest, a significant effect of γ-irradiation on bacterial cells was detected. The absence of genotoxicity at the later stages can be explained by the adaptation of bacterial cells to the conditions of exposure. The findings allow the bacterial test system to be used for studying the effects of low doses at the early stages of exposure to radiation.
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia. radecol@ibp.ru.
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Bolsunovsky
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 10, Novosibirsk, 630090, Russia.|Novosibirsk State University, ul. Pirogova 2, Novosibirsk, 630090, Russia.
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Sinitsyna
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 10, Novosibirsk, 630090, Russia.|Novosibirsk State University, ul. Pirogova 2, Novosibirsk, 630090, Russia.|Vorozhtsov Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 9, Novosibirsk, 630090, Russia.
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Frolova
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 10, Novosibirsk, 630090, Russia.
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Vasyunina
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia.
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Dementyev
D002588:Cesium Radioisotopes; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects*; D005720:Gamma Rays / Q000009:adverse effects*; D009152:Mutagenicity Tests / Q000379:methods*; D013014:SOS Response, Genetics / Q000528:radiation effects; D012486:Salmonella typhimurium / Q000235:genetics* / Q000528:radiation effects*; D013997:Time Factors
D002588:Cesium Radioisotopes