Effect of dietary calcium deficiency and altered diet hardness on the jawbone growth: A micro-CT and bone histomorphometric study in rats.
OBJECTIVE
We examined the effects of a low-calcium diet and altered diet hardness on bone architecture and metabolism in the maxilla and mandible.


METHODS
Male rats (n=48, 3 weeks old) were divided into six groups. In total, 24 rats were given a normal-calcium diet and the others were given a low-calcium diet. Each group was then divided into three subgroups, which were fed a 'hard̕ diet for 8 weeks, a 'soft̕ die for 8 weeks, or switched from the soft diet after 4 weeks to the hard diet for 4 weeks. The bone architecture was analyzed using cephalometry and micro-computed tomography, in addition, the bone metabolism was analyzed using serum bone markers and bone histomorphometry in the maxilla and mandible. Moreover, the bone formation patterns were evaluated using histopathologically in the midpalatal suture.


RESULTS
The low-calcium diet affected bone architecture by increasing bone turnover and the soft diet affected bone architecture mainly by increasing bone resorption. The soft diet changed the chondrocyte cell layers into fibrous connective tissues in the midpalatal suture. At 4 weeks after the return to a hard diet from a soft diet, recovery of the deterioration in bone architectures was seen in the maxilla and mandible.


CONCLUSIONS
We demonstrated that mastication with a hard diet is effective for recovering the collapsed equilibrium of jaw bone turnover and the deteriorating jaw bone architectures due to the poor masticatory function during the growing period.
Division of Developmental Stomatognathic Function Science, Department of Health Promotion, Kyushu Dental University, Kitakyushu, Japan. Electronic address: y-fujita@kyu-dent.ac.jp.
Yuko
Y
Fujita
Division of Developmental Stomatognathic Function Science, Department of Health Promotion, Kyushu Dental University, Kitakyushu, Japan.
Shota
S
Goto
Division of Developmental Stomatognathic Function Science, Department of Health Promotion, Kyushu Dental University, Kitakyushu, Japan.
Maika
M
Ichikawa
Division of Developmental Stomatognathic Function Science, Department of Health Promotion, Kyushu Dental University, Kitakyushu, Japan.
Ayako
A
Hamaguchi
Division of Developmental Stomatognathic Function Science, Department of Health Promotion, Kyushu Dental University, Kitakyushu, Japan.
Kenshi
K
Maki
D000818:Animals; D015415:Biomarkers / Q000097:blood; D016723:Bone Remodeling / Q000187:drug effects*; D001862:Bone Resorption / Q000378:metabolism*; D002136:Calcium, Dietary / Q000008:administration & dosage*; D002508:Cephalometry; D004032:Diet; D006244:Hardness; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging / Q000254:growth & development*; D008437:Maxilla / Q000000981:diagnostic imaging / Q000254:growth & development*; D051381:Rats; D055114:X-Ray Microtomography
D015415:Biomarkers; D002136:Calcium, Dietary
Histomorphometry; Low-calcium diet; Masticatory function; Midpalatal suture; micro-CT