Expression of Inhibitor of Differentiation-1 and Its Effects on Angiogenesis in Gastric Cancer.
OBJECTIVE
To explore the effects of recombinant human endostatin (endostar, ES) and cisplatin on the growth of gastric cancer-transplanted tumor in nude mice and the expression of microvessel density (MVD).


METHODS
Human gastric cancer SGC-7901 cells were subcutaneously injected into the armpit of nude mice to prepare cancer-bearing nude mice. A total of 32 cancer-bearing nude mice were divided into four groups (each group with 8 mice). The four groups included control group and other three groups in which mice were treated with 5 mg/kg of ES (group E), 5 mg/kg of cisplatin (group Ci), and 5 mg/kg of ES combined with 5 mg/kg of cisplatin (group C), respectively. MVD was determined by immunohistochemistry, and the expressions of mRNA and protein of inhibitor of differentiation-1 (ID1) and vascular endothelial growth factor (VEGF) were detected with reverse transcription polymerase chain reaction (RT-PCR) and western blot, respectively. Apoptosis was observed with transmission electron microscope.


RESULTS
Compared with control group, the sizes and weights of tumors were significantly decreased in other three groups (all p < 0.05). MVD was significantly lower in groups E, Ci, and C than in control group, and in groups E and C than in group Ci (all p < 0.05). Compared with control group, the expressions of mRNA and protein of ID1 and VEGF significantly decreased in groups E and C (all p < 0.05). There were no significant differences in the expressions of mRNA and protein of ID1 and VEGF between group Ci and control group. There was apoptosis in groups E and C, but no apoptosis was found in group Ci and control group.


CONCLUSIONS
ES can inhibit the growth of gastric cancer cells through suppressing angiogenesis and promoting apoptosis of tumor cell. This study provides a new idea for the treatment of gastric cancer.
1 Department of Medicine, Shandong University , Shandong, China .|2 Department of General Surgery, the First Affiliated Hospital, Jinzhou Medical University , Jinzhou, China .
Di-Nuo
DN
Li
3 Department of Hepatobiliary Surgery, the First Affiliated Hospital, Jinzhou Medical University , Jinzhou, China .
Liang
L
Wang
4 Department of Cardiology, the First Affiliated Hospital, Jinzhou Medical University , Jinzhou, China .
Lei
L
Wang
5 BioBank, the First Affiliated Hospital, Jinzhou Medical University , Jinzhou, China .
Shen
S
Li
2 Department of General Surgery, the First Affiliated Hospital, Jinzhou Medical University , Jinzhou, China .
Yu-Bing
YB
Wang
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology; D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology; D043169:Endostatins / Q000008:administration & dosage / Q000494:pharmacology; D064593:Heterografts; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D055806:Microvessels / Q000187:drug effects; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D011994:Recombinant Proteins / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000098:blood supply* / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism
D043169:Endostatins; C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; D012333:RNA, Messenger; D011994:Recombinant Proteins; C522911:endostar protein; D002945:Cisplatin
cisplatin; gastric cancer; inhibitor of differentiation-1; recombinant human endostatin