Sp1 transcription factor promotes TMEPAI gene expression and contributes to cell proliferation.
OBJECTIVE
TMEPAI (transmembrane prostate androgen-induced protein) has been reported to be overexpressed during tumour progression; however, little is known concerning transcriptional mechanisms regulating TMEPAI gene expression.


METHODS
In this study, the TMEPAI gene promoter has been identified and characterized, and the effects of Sp1 on TMEPAI-induced viability of A549 cells were evaluated, using MTT and colony formation assays.


RESULTS
We found that the sequence between -298 and +24 consists of basal promoter activity for TMEPAI. Further analysis indicated that two Sp1-binding sites are crucial for maintaining basal transcriptional activity of the TMEPAI promoter, and chromatin immunoprecipitation assays confirmed direct binding of Sp1 to the TMEPAI promoter. In addition, Sp1 up-regulated TMEPAI protein expression, as well as Sp1 promoting TMEPAI-induced cell proliferation.


CONCLUSIONS
These results indicate that the sequence between -298 and +24 consists of the basal promoter activity for TMEPAI. Sp1 promotes TMEPAI expression and contributes to cell proliferation.
Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Yuyin
Y
Li
Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Ailong
A
Guo
Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Yajuan
Y
Feng
Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Yueying
Y
Zhang
Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Jianjun
J
Wang
Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Lifang
L
Jing
Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Yali
Y
Yan
Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Lei
L
Jing
Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Zhenxing
Z
Liu
Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Long
L
Ma
Key Lab of Industrial Fermentation Microbiology of the Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. diaoaipo@tust.edu.cn.
Aipo
A
Diao
D000072283:A549 Cells; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D015533:Transcriptional Activation
D008565:Membrane Proteins; C412868:PMEPA1 protein, human; D016329:Sp1 Transcription Factor