Extensive characterization of a lentiviral-derived stable cell line expressing rabbit hemorrhagic disease virus VPg protein.
Rabbit hemorrhagic disease virus (RHDV) is an important member of the caliciviridae family. Currently, no suitable tissue culture system is available for proliferating RHDV, which limits the study of its pathogenesis. To bypass this obstacle, we established a cell line, RK13-VPg, stably expressing the VPg gene with a lentivirus packaging system in this study. In addition, the recently constructed RHDV replicon in our laboratory provided an appropriate model for studying the pathogenesis of RHDV without in vitro RHDV propagation and culture. Using this RHDV replicon and RK13-VPg cell line, we further demonstrated that the presence of VPg protein is essential for efficient translation of an RHDV replicon. Therefore, the RK13-VPg cell line is a powerful tool for studying the replication and translation mechanisms of RHDV.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, PR China.
Jie
J
Zhu
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, PR China.
Qiuhong
Q
Miao
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, PR China.
Yonggui
Y
Tan
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, PR China.
Huimin
H
Guo
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, PR China.
Chuanfeng
C
Li
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, PR China.
Zongyan
Z
Chen
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, PR China. Electronic address: liugq@shvri.ac.cn.
Guangqing
G
Liu
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D017929:Hemorrhagic Disease Virus, Rabbit / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D011817:Rabbits; D012093:Replicon / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics*; D014779:Virus Replication
D015678:Viral Structural Proteins
Cell line; Rabbit hemorrhagic disease virus; VPg