Enhanced extraction of oleoresin from ginger (Zingiber officinale) rhizome powder using enzyme-assisted three phase partitioning.
Ginger (Zingiber officinale R.) is a popular spice used worldwide. The oleoresin consists of gingerols, shogaols and other non-volatiles as chief bioactive constituents. Three phase partitioning (TPP), a bioseparation technique, based on partitioning of polar constituents, proteins, and hydrophobic constituents in three phases comprising of water, ammonium sulphate and t-butanol, was explored for extraction of oleoresin and gingerols from dry powder. Parameters optimized for maximum recovery of gingerols and [6]-shogaol were ammonium sulphate concentration, ratio of t-butanol to slurry, solid loading and pH. Ultrasound and enzymatic pretreatments increased the yield of oleoresin and its phytoconstituents. Ultrasound pretreatment showed separation of starch in the bottom aqueous phase but is an additional step in extraction. Enzymatic pretreatment using accellerase increased the yield of [6]-, [8]-, [10]-gingerols and [6]-shogaol by 64.10, 87.8, 62.78 and 32.0% within 4h and is recommended. The efficacy of the enzymatic pretreatment was confirmed by SEM and FTIR.
Food Engineering and Technology Department, Institute of Chemical Technology, Matunga, Mumbai 400 019, India. Electronic address: svarakumar@gmail.com.
Sadineni
S
Varakumar
Food Engineering and Technology Department, Institute of Chemical Technology, Matunga, Mumbai 400 019, India.
Kannamangalam Vijayan
KV
Umesh
Food Engineering and Technology Department, Institute of Chemical Technology, Matunga, Mumbai 400 019, India.
Rekha S
RS
Singhal
D002396:Catechols / Q000032:analysis; D005233:Fatty Alcohols / Q000032:analysis; D020939:Zingiber officinale / Q000737:chemistry*; D064209:Phytochemicals / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis*; D028321:Plant Preparations / Q000032:analysis*; D011208:Powders / Q000032:analysis; D027343:Rhizome / Q000737:chemistry*
D002396:Catechols; D005233:Fatty Alcohols; D064209:Phytochemicals; D010936:Plant Extracts; D028321:Plant Preparations; D011208:Powders; C018620:oleoresins; C040115:shogaol; C007845:gingerol
Enzyme assisted three phase partitioning; Ginger oleoresin; Three phase partitioning; Ultrasound assisted three phase partitioning; Zingiber officinale Roscoe