Determination of genotoxic effects of methidathion alkaline hydrolysis in human lymphocytes using the micronucleus assay and square-wave voltammetry.
The interaction of pesticides with environmental factors, such as pH, may result in alterations of their physicochemical properties and should be taken into consideration in regard to their classification. This study investigates the genotoxicity of methidathion and its alkaline hydrolysis by-products in cultured human lymphocytes, using the square-wave voltammetry (square wave-adsorptive cathodic stripping voltammetry (SW-AdCSV) technique) and the cytokinesis block micronucleus assay (CBMN assay). According to the SW-AdCSV data the alkaline hydrolysis of methidathion results in two new molecules, one non-electro-active and a second electro-active which is more genotoxic than methidathion itself in cultured human lymphocytes, inducing higher micronuclei frequencies. The present study confirms the SW-AdCSV technique as a voltammetric method which can successfully simulates the electrodynamics of the cellular membrane.
Center of Toxicology Science & Research, Division of Morphology, Medical School, University of Crete, Voutes Campus, Heraklion, 71003, Crete, Greece.
Polychronis D
PD
Stivaktakis
School of Science and Technology, Hellenic Open University, Tsamadou 13-15 & Saint Andrea, 262 22 Patras, Greece. Electronic address: v.giann@yahoo.com.
Evangelos
E
Giannakopoulos
Department of Environmental and Natural Resources Management, University of Patras, Seferi 2, 30100 Agrinio, Greece.
Dimitris
D
Vlastos
Department of Environmental and Natural Resources Management, University of Patras, Seferi 2, 30100 Agrinio, Greece.
Demetrios P
DP
Matthopoulos
D000328:Adult; D002462:Cell Membrane / Q000187:drug effects; D048749:Cytokinesis / Q000187:drug effects; D004563:Electrochemistry; D006801:Humans; D006868:Hydrolysis; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009946:Organothiophosphorus Compounds / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity*; D055815:Young Adult
D009946:Organothiophosphorus Compounds; C005828:methidathion
Genotoxicity; HMDE; Hydrolysis; Methidathion; Micronucleus assay; Square-wave voltammetry