Stress differentially regulates brain expression of corticotropin-releasing factor in binge-like eating prone and resistant female rats.
The expression of corticotropin-releasing factor (CRF), a neuropeptide that regulates endocrine and behavioral responses to stress, was assessed in the brain in rats prone or resistant to stress-induced binge-like eating of sucrose. Female Sprague-Dawley rats were subjected to unpredictable intermittent 1-h access to sucrose in non-stressful conditions or after exposure to three foot shock stress sessions. Experimental sessions were performed at metestrus, diestrus, and proestrus. The rats were assigned to the binge-like eating prone (BEP) or the binge-like eating resistant (BER) phenotypes according to the rats' persistently high or low sucrose intake following three stress sessions. The BEP rats displayed elevated consumption of sucrose in non-stressful conditions and an additional significant increase in sucrose intake in response to stress. Conversely, the BER rats showed lower sucrose intake in non-stressful conditions, and stress did not increase sucrose intake in this phenotype. The brain expression of CRF mRNA and plasma corticosterone levels were assessed 30 min after the last stress session at the diestrous phase of the estrous cycle. Stress triggered a significant increase in plasma corticosterone levels and strongly increased CRF mRNA expression in the paraventricular hypothalamic nucleus in the BER but not in the BEP rats. However, the BEP but not the BER rats demonstrated a significant increase in CRF mRNA expression in the bed nucleus of the stria terminalis (BNST) after stress. Hyporeactivity of the hypothalamic-pituitary-adrenal axis and the higher CRF expression in the BNST in BEP rats may contribute to stress-induced binge-like sucrose eating in the BEP phenotype.
Faculté de Médecine, Département de Psychiatrie et de Neurosciences, Centre de Recherche de L'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC G1V 0A6, Canada.
Juliane
J
Calvez
Faculté de Médecine, Département de Psychiatrie et de Neurosciences, Centre de Recherche de L'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC G1V 0A6, Canada.
Camila
C
de Ávila
Faculté de Médecine, Département de Psychiatrie et de Neurosciences, Centre de Recherche de L'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC G1V 0A6, Canada.
Geneviève
G
Guèvremont
Faculté de Médecine, Département de Psychiatrie et de Neurosciences, Centre de Recherche de L'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC G1V 0A6, Canada. Electronic address: Elena.Timofeeva@fmed.ulaval.ca.
Elena
E
Timofeeva
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002032:Bulimia / Q000378:metabolism* / Q000523:psychology*; D003345:Corticosterone / Q000097:blood; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D030762:Estrous Cycle; D005260:Female; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000378:metabolism*; D013395:Sucrose / Q000008:administration & dosage; D013549:Sweetening Agents / Q000008:administration & dosage
D012333:RNA, Messenger; D013549:Sweetening Agents; D013395:Sucrose; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone
BNST; Binge eating; CRF; PVN; Stress; Sucrose