Quantitative bioimaging of platinum group elements in tumor spheroids.
Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method.
Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 30, 48149 Münster, Germany.
Ann-Christin
AC
Niehoff
Department of Pharmaceutical Technology and Biopharmacy, University of Münster, Corrensstr. 48, 48149 Münster, Germany.
Jonas
J
Grünebaum
Department of Bioprocessing & Bioanalytics, Fraunhofer Institute for Biomedical Engineering, Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany.
Aline
A
Moosmann
Department of Pharmaceutical Technology and Biopharmacy, University of Münster, Corrensstr. 48, 48149 Münster, Germany.
Dennis
D
Mulac
Department of Pharmaceutical Technology and Biopharmacy, University of Münster, Corrensstr. 48, 48149 Münster, Germany.
Judith
J
Söbbing
Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 30, 48149 Münster, Germany.
Rebecca
R
Niehaus
Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 30, 48149 Münster, Germany.
Rebecca
R
Buchholz
Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 30, 48149 Münster, Germany.
Sabrina
S
Kröger
Biolitec Research GmbH, Otto-Schott-Str. 15, 07745 Jena, Germany.
Arno
A
Wiehe
Department of Bioprocessing & Bioanalytics, Fraunhofer Institute for Biomedical Engineering, Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany.
Sylvia
S
Wagner
Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 30, 48149 Münster, Germany.
Michael
M
Sperling
Department of Bioprocessing & Bioanalytics, Fraunhofer Institute for Biomedical Engineering, Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany.
Hagen
H
von Briesen
Department of Pharmaceutical Technology and Biopharmacy, University of Münster, Corrensstr. 48, 48149 Münster, Germany.
Klaus
K
Langer
Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 30, 48149 Münster, Germany. Electronic address: uk@uni-muenster.de.
Uwe
U
Karst
D045744:Cell Line, Tumor; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009369:Neoplasms / Q000737:chemistry* / Q000378:metabolism; D010165:Palladium / Q000737:chemistry / Q000493:pharmacokinetics; D017671:Platinum Compounds / Q000032:analysis*
D017671:Platinum Compounds; D010165:Palladium
LA-ICP-MS; Photosensitizer; Pt cytostatics; Quantification; Tumor spheroids