Plasmon-Enhanced Photocleaving Dynamics in Colloidal MicroRNA-Functionalized Silver Nanoparticles Monitored with Second Harmonic Generation.
The photocleaving dynamics of colloidal microRNA-functionalized nanoparticles are studied using time-dependent second harmonic generation (SHG) measurements. Model drug-delivery systems composed of oligonucleotides attached to either silver nanoparticles or polystyrene nanoparticles using a nitrobenzyl photocleavable linker are prepared and characterized. The photoactivated controlled release is observed to be most efficient on resonance at 365 nm irradiation, with pseudo-first-order rate constants that are linearly proportional to irradiation powers. Additionally, silver nanoparticles show a 6-fold plasmon enhancement in photocleaving efficiency over corresponding polystyrene nanoparticle rates, while our previous measurements on gold nanoparticles show a 2-fold plasmon enhancement compared to polystyrene nanoparticles. Characterizations including extinction spectroscopy, electrophoretic mobility, and fluorimetry measurements confirm the analysis from the SHG results. The real-time SHG measurements are shown to be a highly sensitive method for investigating plasmon-enhanced photocleaving dynamics in model drug delivery systems.
Raju R
RR
Kumal
Mohammad
M
Abu-Laban
Corey R
CR
Landry
Blake
B
Kruger
Zhenyu
Z
Zhang
Department of Biomedical Engineering, the Pennsylvania State University , University Park, Pennsylvania 16802, United States.
Daniel J
DJ
Hayes
Louis H
LH
Haber
D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry / Q000528:radiation effects*; D065546:Drug Liberation; D007700:Kinetics; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry / Q000528:radiation effects*; D035683:MicroRNAs / Q000737:chemistry / Q000528:radiation effects*; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry / Q000528:radiation effects*; D000073798:Second Harmonic Generation Microscopy; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance
D004337:Drug Carriers; D035683:MicroRNAs; D011137:Polystyrenes; D012834:Silver