Cloning and heterologous expression of a novel subgroup of class IV polyhydroxyalkanoate synthase genes from the genus Bacillus.
Many microorganisms harbor genes necessary to synthesize biodegradable plastics known as polyhydroxyalkanoates (PHAs). We surveyed a genomic database and discovered a new cluster of class IV PHA synthase genes (phaRC). These genes are different in sequence and operon structure from any previously reported PHA synthase. The newly discovered PhaRC synthase was demonstrated to produce PHAs in recombinant Escherichia coli.
a Department of Creative Engineering , Kitakyushu National College of Technology , Kitakyushu , Japan.
Kouhei
K
Mizuno
b Department of Innovative and Engineered Materials , Tokyo Institute of Technology , Yokohama , Japan.
Takahiro
T
Kihara
b Department of Innovative and Engineered Materials , Tokyo Institute of Technology , Yokohama , Japan.
Takeharu
T
Tsuge
c Department of Chemistry , College of Environmental Science and Forestry, State University of New York , Syracuse , NY , USA.
Benjamin R
BR
Lundgren
c Department of Chemistry , College of Environmental Science and Forestry, State University of New York , Syracuse , NY , USA.
Zaara
Z
Sarwar
c Department of Chemistry , College of Environmental Science and Forestry, State University of New York , Syracuse , NY , USA.
Atahualpa
A
Pinto
c Department of Chemistry , College of Environmental Science and Forestry, State University of New York , Syracuse , NY , USA.|d Center for Applied Microbiology , College of Environmental Science and Forestry, State University of New York , Syracuse , NY , USA.|e Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources , College of Life Sciences, Hubei University , Wuhan , People's Republic of China.
Christopher T
CT
Nomura
D000217:Acyltransferases / Q000235:genetics*; D001407:Bacillus / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D030541:Databases, Genetic; D015870:Gene Expression; D010802:Phylogeny
D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase
Bacillus; biodegradable plastic; class IV PHA synthase; novel subgroup; polyhydroxyalkanoates