Transcranial theta-burst stimulation alters GLT-1 and vGluT1 expression in rat cerebellar cortex.
Repetitive transcranial magnetic stimulation (rTMS) induces changes in expression of proteins engaged in activity of excitatory and inhibitory systems as well as redox homeostasis. Our aim was to investigate the effect of single (SS) and repeated session (RS) of intermittent and continuous theta-burst stimulation (iTBS; cTBS) on the expression of vesicular and plasmatic glutamate transporters 1 (vGluT1 and GLT-1), glial fibrillary acidic protein (GFAP) and influence on oxidative status in rats cerebellar tissue and plasma. Redox state parameters in cerebellar tissue and plasma were assessed 24 h after single and 48 h after the last TBS session. Molecular changes were examined by immunofluorescence. Stimulation significantly increased thiol groups (SH) in tissue of SS iTBS group, and decreased in iTBS RS. Activity of glucose-6-phosphate-dehydrogenase (G6PD) was increased markedly in cTBS RS. Immunoreactivity of vGluT1 in cTBS RS decreased, while GLT-1 increased in cTBS SS and cTBS RS, compared to control. Present study gives insight in molecular and biochemical mechanisms by which iTBS and cTBS exerts its effects on rats cerebellar cortex.
Medical Faculty of Military Medical Academy, Crnotravska 17, University of Defense, 11000, Belgrade, Serbia. Electronic address: malicevic.bojana@gmail.com.
Bojana
B
Mancic
Medical Faculty of Military Medical Academy, Crnotravska 17, University of Defense, 11000, Belgrade, Serbia; Institute of Medical Research, Medical Faculty of Military Medical Academy, Crnotravska 17, University of Defense, 11000, Belgrade, Serbia. Electronic address: ivanav13@yahoo.ca.
Ivana
I
Stevanovic
Medical Faculty of Military Medical Academy, Crnotravska 17, University of Defense, 11000, Belgrade, Serbia; Department of Neurophysiology, Medical Faculty of Military Medical Academy, Crnotravska 17, University of Defense, 11000, Belgrade, Serbia. Electronic address: tihoilic@gmail.com.
Tihomir V
TV
Ilic
Department of Toxicology, Faculty of Pharmacy, Vojvode Stepe 450, University of Belgrade, 11221, Belgrade, Serbia. Electronic address: ana.jelicmariokov@gmail.com.
Ana
A
Djuric
Institute of Biochemistry, Faculty of Medicine Bulevar Dr Zorana Đinđića 81, University of Nis, 18000, Nis, Serbia. Electronic address: stojanovicivana38@gmail.com.
Ivana
I
Stojanovic
Institute of Medical Research, PO Box 102, University of Belgrade, 11129, Belgrade, Serbia. Electronic address: sladjan.milanovic@gmail.com.
Sladjan
S
Milanovic
Medical Faculty of Military Medical Academy, Crnotravska 17, University of Defense, 11000, Belgrade, Serbia; Institute of Medical Research, Medical Faculty of Military Medical Academy, Crnotravska 17, University of Defense, 11000, Belgrade, Serbia. Electronic address: ninkovic7@gmail.com.
Milica
M
Ninkovic
D000818:Animals; D002525:Cerebellar Cortex / Q000378:metabolism*; D027342:Excitatory Amino Acid Transporter 2 / Q000378:metabolism*; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D008297:Male; D017208:Rats, Wistar; D050781:Transcranial Magnetic Stimulation / Q000379:methods
D027342:Excitatory Amino Acid Transporter 2; D051272:Glucose Transporter Type 1; C493395:Slc1a2 protein, rat; C495679:Slc2a1 protein, rat
Cerebellum; GLT-1; Glutathion; Rat; rTMS; vGluT1