Humanized mouse as an appropriate model for accelerated global HIV research and vaccine development: current trend.
Humanized mouse models currently have seen improved development and have received wide applications. Its usefulness is observed in cell and tissue transplant involving basic and applied human disease research. In this article, the development of a new generation of humanized mice was discussed as well as their relevant application in HIV disease. Furthermore, current techniques employed to overcome the initial limitations of mouse model were reviewed. Highly immunodeficient mice which support cell and tissue differentiation and do not reject xenografts are indispensable for generating additional appropriate models useful in disease study, this phenomenom deserves emphases, scientific highlight and a definitive research focus. Since the early 2000s, a series of immunodeficient mice appropriate for generating humanized mice has been successively developed by introducing the IL-2Rγnullgene (e.g. NOD/SCID/γcnulland Rag2nullγcnullmice) through various genomic approaches. These mice were generated by genetically introducing human cytokine genes into NOD/SCID/γcnulland Rag2nullγcnullmouse backgrounds. The application of these techniques serves as a quick and appropriate mechanistic model for basic and therapeutic investigations of known and emerging infections.
a Immunovirology and Vaccine Development Laboratory, Medical Biotechnology Department , National Biotechnology Development Agency , Abuja , Nigeria.
Bartholomew Okechukwu
BO
Ibeh
b RIKEN CDB CLST (Center for Life Science Technologies) , Kobe , Japan.
Yasuhide
Y
Furuta
c Bioresources Development Center Odi, Bayelsa , National Biotechnology Development Agency , Abuja , Nigeria.
Josiah Bitrus
JB
Habu
d National Biotechnology Development Agency , Abuja , Nigeria.
Lucy
L
Ogbadu
CRISPR/Cas; HIV; Mouse; genetic; genomic; immunologic; models; transgenic