Gadolinium oxide: Exclusive selectivity and sensitivity in the enrichment of phosphorylated biomolecules.
Selectivity and sensitivity define the dynamic applicability of separation and enrichment techniques. Owing to proteome complexity, numbers of separation media have been introduced in phosphoproteomics. Complex samples are pretreated to make the low-abundance molecules detectable by mass spectrometry. Gadolinium oxide nanoparticles, offering mono- and bi-dentate interactions, are optimized to capture the phosphopeptides. Selectivity of 1:11 000 is achieved for digested β-casein phosphopeptides in bovine serum albumin digest background using gadolinium oxide nanoparticles. The limit of detection goes down to 1 attomole. With the optimized sample preparation protocol, gadolinium oxide nanoparticles enrich phosphopeptides of κ-casein (Ser148and Ser170) from digested milk sample, fibrinogen alpha chain phosphopeptide (Ser609) along with four hydrolytic products of Ser22-modified phosphopeptides from serum.
Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan.|Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innsbruck, Austria.
Fahmida
F
Jabeen
Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan.|Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innsbruck, Austria.
Muhammad
M
Najam-Ul-Haq
Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan.
Muhammad Naeem
MN
Ashiq
Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innsbruck, Austria.
Matthias
M
Rainer
Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innsbruck, Austria.
Christian W
CW
Huck
Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innsbruck, Austria.
Guenther K
GK
Bonn
D000818:Animals; D002364:Caseins / Q000302:isolation & purification*; D005682:Gadolinium / Q000737:chemistry*; D008892:Milk; D053758:Nanoparticles / Q000737:chemistry*; D010748:Phosphopeptides / Q000302:isolation & purification*; D044967:Serum; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002364:Caseins; D010748:Phosphopeptides; C030581:gadolinium oxide; D005682:Gadolinium
Affinity chromatography; Gadolinium oxide; Mass spectrometry; Nanoparticles; Phosphopeptides