Deinococcus saudiensis sp. nov., isolated from desert.
Two gamma- and UV-radiation-resistant, pink-coloured bacterial strains, designated YIM F302T and YIM F235, were isolated from the desert of Yanbu' al Bahr located in west of Saudi Arabia. Taxonomic positions of the two isolates were investigated by polyphasic taxonomic approaches. Cells of the two strains were Gram-stain-negative, aerobic and rod-shaped. They were able to grow at 15-45 °C and pH 6.0-8.0 and had a NaCl tolerance limit of 1 % (w/v). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strains YIM F302T and YIM F235 represent members of the genus Deinococcus, sharing highest sequence similarities of 98.3 and 98.4 %, respectively, with Deinococcus grandis DSM 3963T. The strains were found to contain MK-8 as the respiratory menaquinone. Major fatty acids (>10 %) of the two strains were C15 : 1ω6c, C16 : 0 and C16 : 1ω7c. DNA-DNA hybridization values of the two isolates against the closely related type strains were significantly below the 70 % limit for species delineation. Genomic DNA G+C contents of strains YIM F302T and YIM F235 were 69.3 and 69.0 mol%, respectively. Based on the phenotypic and genotypic characteristics recorded, it is determined that the two isolates represent a novel species of the genus Deinococcus, for which the name Deinococcus saudiensis sp. nov. is proposed. The type strain is YIM F302T (=CGMCC 1.15089T=DSM 29933T).
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, School of Life Science, Yunnan University, Kunming 650091, PR China.
Firasat
F
Hussain
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, School of Life Science, Yunnan University, Kunming 650091, PR China.
Inam Ullah
IU
Khan
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, School of Life Science, Yunnan University, Kunming 650091, PR China.
Neeli
N
Habib
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Wen-Dong
WD
Xian
Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Wael N
WN
Hozzein
Xinjiang Laboratory of Special Environmental Microbiology, Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China.
Zhi-Dong
ZD
Zhang
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, School of Life Science, Yunnan University, Kunming 650091, PR China.
Xiao-Yang
XY
Zhi
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.|Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, School of Life Science, Yunnan University, Kunming 650091, PR China.
Wen-Jun
WJ
Li
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D034301:Deinococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003889:Desert Climate; D005227:Fatty Acids / Q000737:chemistry; D005720:Gamma Rays; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012529:Saudi Arabia; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014466:Ultraviolet Rays; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8