Metabolic flux analysis of carbon balance in Lactobacillus strains.
Metabolic flux analyses were performed based on the carbon balance of six different Lactobacillus strains used in this study. Results confirmed that L. delbrueckii, L. plantarum ATCC 21028, L. plantarum NCIMB 8826 ΔldhL1, L. plantarum NCIMB 8826 ΔldhL1-pCU-PxylAB, and L. plantarum NCIMB 8826 ΔldhL1-pLEM415-xylAB metabolized glucose via EMP: whereas, L. brevis metabolized glucose via PK pathway. Xylose was metabolized through the PK pathway in L. brevis, L. plantarum NCIMB 8826 ΔldhL1-pCU-PxylAB and L. plantarum NCIMB 8826 ΔldhL1-pLEM415-xylAB. Operation of both EMP and PK pathways was found in L. brevis, L. plantarum NCIMB 8826 ΔldhL1-pCU-PxylAB, and L. plantarum NCIMB 8826 ΔldhL1-pLEM415-xylAB when glucose plus xylose were used as carbon source. The information of detailed carbon flow may help the strain and biomass selection in a designed process of lactic acid biosynthesis. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1397-1403, 2016.
Bioprocessing and Renewable Energy Laboratory, Department of Grain Science and Industry, Kansas State University, Manhattan, Kansas.
Yixing
Y
Zhang
Dept. of Chemical Engineering, Kansas State University, Manhattan, Kansas.
Fan
F
Zeng
Dept. of Chemical Engineering, Kansas State University, Manhattan, Kansas.
Keith
K
Hohn
Dept. of Chemical Engineering, Kansas State University, Manhattan, Kansas.|Dept. of Grain Science and Industry, Bioprocessing and Renewable Energy Laboratory, Kansas State University, Manhattan, Kansas.
Praveen V
PV
Vadlani
D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis / Q000737:chemistry; D007778:Lactobacillus / Q000378:metabolism*; D064688:Metabolic Flux Analysis
D019344:Lactic Acid; D002244:Carbon
Embden-Meyerhof pathway; Lactobacillus brevis phosphoketolase pathway; Lactobacillus delbrueckii; Lactobacillus plantarum