Zwitterionic hydrophilic interaction solid-phase extraction and multi-dimensional mass spectrometry for shotgun lipidomic study of Hypophthalmichthys nobilis.
Zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) material was used as solid-phase extraction sorbent for purification of phospholipids from Hypophthalmichthys nobilis. The conditions were optimized to be pH 6, flow rate 2.0mL·min(-1), loading breakthrough volume ⩽5mL, and eluting solvent 5mL. Afterwards, the extracts were analyzed by multi-dimensional mass spectrometry (MDMS) based shotgun lipidomics; 20 species of phosphatidylcholine (PC), 22 species of phosphatidylethanoamine (PE), 15 species of phosphatidylserine (PS), and 5 species of phosphatidylinositol (PI) were identified, with content 224.1, 124.1, 27.4, and 34.7μg·g(-1), respectively. The MDMS method was validated in terms of linearity (0.9963-0.9988), LOD (3.7ng·mL(-1)), LOQ (9.8ng·mL(-1)), intra-day precision (<3.64%), inter-day precision (<5.31%), and recovery (78.8-85.6%). ZIC-HILIC and MDMS shotgun lipidomics are efficient for studying phospholipids in H. nobilis.
Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China.
Renyao
R
Jin
School of Public Health, Guangdong Medical University, Dongguan, China.
Linqiu
L
Li
Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China.
Junli
J
Feng
Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China.
Zhiyuan
Z
Dai
Department of Food Science and Technology, University of Georgia, Athens, GA, United States.
Yao-Wen
YW
Huang
Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China. Electronic address: leonqshen@163.com.
Qing
Q
Shen
D000818:Animals; D002347:Carps / Q000378:metabolism*; D002853:Chromatography, Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry / Q000379:methods*; D010743:Phospholipids / Q000032:analysis* / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000379:methods*
D010743:Phospholipids
Hypophthalmichthys nobilis; Multi-dimensional mass spectrometry; Shotgun lipidomics; Solid-phase extraction; Zwitterionic hydrophilic interaction liquid chromatography