(1)H, (13)C, (15)N backbone and side-chain resonance assignment of Nostoc sp. C139A variant of the heme-nitric oxide/oxygen binding (H-NOX) domain.
The H-NOX (Heme-nitric oxide/oxygen binding) domain is conserved across eukaryotes and bacteria. In human soluble guanylyl cyclase (sGC) the H-NOX domain functions as a sensor for the gaseous signaling agent nitric oxide (NO). sGC contains the heme-binding H-NOX domain at its N-terminus, which regulates the catalytic site contained within the C-terminal end of the enzyme catalyzing the conversion of GTP (guanosine 5'-triphosphate) to GMP (guanylyl monophosphate). Here, we present the backbone and side-chain assignments of the (1)H, (13)C and (15)N resonances of the 183-residue H-NOX domain from Nostoc sp. through solution NMR.
Department of Pharmacy, University of Patras, 26504, Patras, Greece.
Ioannis I
II
Alexandropoulos
Department of Pharmacy, University of Patras, 26504, Patras, Greece.
Aikaterini I
AI
Argyriou
Department of Pharmacy, University of Patras, 26504, Patras, Greece.
Kostas D
KD
Marousis
Department of Pharmacy, Laboratory of Molecular Pharmacology, University of Patras, 26504, Patras, Greece.
Stavros
S
Topouzis
Department of Pharmacy, Laboratory of Molecular Pharmacology, University of Patras, 26504, Patras, Greece.|Faculty of Pharmacy, National and Kapodistrian University of Athens, 15 771, Athens, Greece.
Andreas
A
Papapetropoulos
Department of Pharmacy, University of Patras, 26504, Patras, Greece. G.A.Spyroulias@upatras.gr.
Georgios A
GA
Spyroulias
D000595:Amino Acid Sequence; D006418:Heme / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D046937:Nostoc / Q000201:enzymology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010100:Oxygen / Q000378:metabolism*; D000072417:Protein Domains; D000071756:Soluble Guanylyl Cyclase / Q000737:chemistry* / Q000378:metabolism
D009569:Nitric Oxide; D006418:Heme; D000071756:Soluble Guanylyl Cyclase; D010100:Oxygen
H-NOX; NMR spectroscopy; Nitric oxide binding domain; Recombinant protein expression; Soluble guanylyl cyclase; sGC