Chitosan derivatives/reduced graphene oxide/alginate beads for small-molecule drug delivery.
This work reported chitosan derivatives (CSD)/reduced graphene oxide (rGO) blending with alginate to prepare hydrogel beads for small-molecule drug delivery for the first time. At the beginning, graphene oxide (GO) was successfully reduced using diverse CSD as reducing and stabilizing agents via facile heating. Then the obtained CSD/rGO was blended with alginate and crosslinked into hydrogel beads in CaCl2 solution. Finally, the beads were systematically evaluated as novel vehicles for pH-responsive small-molecule drug delivery. The optimal CSD/rGO/alginate beads showed a high drug-loading efficiency of 82.8% on small-molecule fluorescein sodium (FL), outstanding sustainable release of 71.6% upon 150h at a physiological pH and quick-release of 82.4% drug content at 20h in an acidic medium. Additionally, the cytotoxicity assay result suggested that the CSD/rGO/alginate beads showed negligible cytotoxicity to hepatic stellate cell lines, opening up possibilities for safe and efficient drug delivery.
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
Kaihang
K
Chen
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
Yunzhi
Y
Ling
The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510275, China.
Cong
C
Cao
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China; Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston 02139, USA.
Xiaoyun
X
Li
The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510275, China.
Xiao
X
Chen
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China; Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston 02139, USA. Electronic address: xyw@scut.edu.cn.
Xiaoying
X
Wang
D000464:Alginates / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D065546:Drug Liberation; D019793:Fluorescein / Q000737:chemistry / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000187:drug effects; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D013059:Spectrum Analysis, Raman
D000464:Alginates; D004337:Drug Carriers; D006603:Hexuronic Acids; D010087:Oxides; D006108:Graphite; D020723:Glucuronic Acid; D048271:Chitosan; D019793:Fluorescein
Alginate; Beads; Chitosan derivatives; Drug delivery; Fluorescein sodium; Reduced graphene oxide