Vinegar amount in the process affected the components of vinegar-baked Radix Bupleuri and its hepatoprotective effect.
BACKGROUND
Bupleuri Radix (in Chinese Chaihu), the dried roots of Bupleurum Chinense DC, is a traditional Chinese medicine widely used to treat fever, hepatitis, jaundice, nephritis, dizziness. When baked with vinegar, its effect is more focused on liver related disease. This paper was undertaken to determine the best vinegar amount in the processing and explore its key efficacy components.


METHODS
Hepatoprotective effects of Radix Bupleuri after processing with different amount of vinegar (1:5, 2:5, 3:5) were investigated on liver hurt rats, and the change of constituents were analyzed by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS).


RESULTS
With the increasing amount of vinegar, the hepatoprotective effects of vinegar-baked Radix Bupleuri (VBRB) and the content of saikosaponin b2 increased.


CONCLUSIONS
These results suggested that vinegar amount in the process affected the pharmacological effect of VBRB significantly and saikosaponin b2 may be the key efficacy component of it.
Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Neihuan Xilu, Guangzhou Daxuecheng, Guangzhou, 510006, China.|The postdoctoral research station, Guangzhou University of Chinese Medicine, Neihuan Xilu, Guangzhou Daxuecheng, Guangzhou, 510006, China.
Ya
Y
Zhao
Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Neihuan Xilu, Guangzhou Daxuecheng, Guangzhou, 510006, China.
Yin-Jie
YJ
Wang
Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Neihuan Xilu, Guangzhou Daxuecheng, Guangzhou, 510006, China. 13610241754@163.com.
Rui-Zhi
RZ
Zhao
Yipinhong Pharmaceutical Co. Ltd, 17rd floor, Xinghui International Building, New city Zhujiang, Guangzhou, 510623, China. elephe@126.com.
Fei-Jun
FJ
Xiang
D019342:Acetic Acid / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives; D018517:Plant Roots / Q000737:chemistry*; D020011:Protective Agents / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012503:Saponins
D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D012503:Saponins; D009828:Oleanolic Acid; D019342:Acetic Acid; C025759:saikosaponin D
Hepatoprotective effect; Saikosaponin; Vinegar amount; Vinegar-baked Radix Bupleuri