A polymorphism at the microRNA binding site in the 3'-untranslated region ofC14orf101is associated with the risk of gastric cancer development.
MicroRNAs (miRNAs) bind to the 3'-untranslated regions (3'-UTRs) of mRNAs, affecting translation and regulating cell differentiation, tumorigenesis and apoptosis. Genetic polymorphisms in these regions in target genes are able to affect the binding affinity between miRNA and target genes, ultimately affecting the expression of individual miRNAs. In the present case-control study, genotyping of 5 microRNA single nucleotide polymorphisms (SNPs) located at the binding site of the 3'-UTR ofRYR3(rs1044129),C14orf101(rs4901706),KIAA0423(rs1053667),GOLGA7(rs11337) andKRT81(rs3660) genes was assessed in order to investigate its role in gastric cancer (GC). The results indicated that the rs4901706 SNP, which is located in the 3'-UTR ofC14orf101, was associated with GC development risk, as determined by χ2analysis (relative risk, 1.630; 95% confidence interval, 1.070-2.483; P=0.022). ARenilla/luciferase reporter assay also indicated the different binding affinity between the SNP of rs4901706 and microRNA. In conclusion, rs4901706 SNP ofC14orf101gene in the microRNA binding site may be used as a valuable biomarker when predicting GC risk.
Department of Gynaecology Ultrasound, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.
Cuiju
C
Wang
Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.
Yufei
Y
Zhao
Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.
Yanming
Y
Ming
Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.
Shengnan
S
Zhao
Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.
Zhanjun
Z
Guo
C14orf101; cancer risk; gastric cancer; rs4901706; single nucleotide polymorphism