Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method.
IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using Hevylite™ antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was observed (increased in 16 and decreased in 3 cases). The analysis of light chain composition of intrathecally synthesised immunoglobulins could contribute to our understanding of intrathecal humoral immune response, although its diagnostic utility is limited.
a Institute of Laboratory Diagnostics , Fakultni Nemocnice Ostrava , Ostrava , Czech Republic.|b Clinic of Neurology , Fakultni Nemocnice Ostrava , Ostrava , Czech Republic.
David
D
Zeman
c Department of Clinical Biochemistry , Institute of Laboratory Medicine , Ostrava , Czech Republic.
Pavlína
P
Kušnierová
d Institute for Healthcare Disciplines, St. Elizabeth University College of Health and Social Work in Bratislava , Bratislava , Slovakia.
Jana
J
Bojková
c Department of Clinical Biochemistry , Institute of Laboratory Medicine , Ostrava , Czech Republic.
František
F
Všianský
b Clinic of Neurology , Fakultni Nemocnice Ostrava , Ostrava , Czech Republic.
Olga
O
Zapletalová
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid* / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000134:cerebrospinal fluid* / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000134:cerebrospinal fluid* / Q000276:immunology
D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains
ELISA; cerebrospinal fluid; immunoglobulins; isoectric focusing; kappa/lambda ratio