Delineation of the central melanocortin circuitry controlling the kidneys by a virally mediated transsynaptic tracing study in transgenic mouse model.
To examine if brain neurons involved in the efferent control of the kidneys possess melanocortin-4 receptor (MC4-R) and/or tryptophan hydroxylase (TPH). Retrograde tracing pseudorabies virus (PRV)-614 was injected into the kidneys in adult male MC4R-green fluorescent protein (GFP) transgenic mice. After a survival time of 3-7 days, spinal cord and brain were removed and sectioned, and processed for PRV-614 visualization. The neurochemical phenotype of PRV-614-positive neurons was identified using double or triple immunocytochemical labeling against PRV-614, MC4R, or TPH. Double and triple labeling was quantified using microscopy. The majority of PRV-614 immunopositive neurons which also expressed immunoreactivity for MC4R were located in the ipsilateral intermediolateral cell column (IML) of the thoracic spinal cord, the paraventricular nucleus (PVN) of the hypothalamus, and raphe pallidus (RPa), nucleus raphe magnus (NRM) and ventromedial medulla (VMM) of the brainstem. Triple-labeled MC4R/PRV-614/TPH neurons were concentrated in the PVN, RPa, NRM and VMM. These data strongly suggest that central MC4R and TPH are involved in the efferent neuronal control of the kidneys.
Department of Anesthesiology and Pain Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
Tao Tao
TT
Liu
Department of Anesthesiology and Pain Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
Bao Wen
BW
Liu
Department of Anesthesiology and Pain Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
Zhi Gang
ZG
He
Department of Anesthesiology and Pain Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
Li
L
Feng
Department of Hepatobiliary Surgery, The Second Hospital, Hebei Medical University, Shijiazhuang, People's Republic of China.
San Guang
SG
Liu
Department of Anesthesiology and Pain Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
Hong Bing
HB
Xiang
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism / Q000821:virology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D011558:Herpesvirus 1, Suid / Q000502:physiology; D054884:Host-Pathogen Interactions; D007150:Immunohistochemistry / Q000379:methods; D007668:Kidney / Q000294:innervation / Q000378:metabolism* / Q000821:virology; D008297:Male; D008822:Mice, Transgenic; D009434:Neural Pathways / Q000378:metabolism* / Q000821:virology; D009474:Neurons / Q000378:metabolism; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism / Q000821:virology; D014365:Tryptophan Hydroxylase / Q000378:metabolism
D044105:Receptor, Melanocortin, Type 4; D049452:Green Fluorescent Proteins; D014365:Tryptophan Hydroxylase
Pathology Section; central melanocortin circuitry; kidney; melanocortin-4 receptor; neurochemical phenotype; pseudorabies virus