A novel bioremediation strategy for petroleum hydrocarbon pollutants using salt tolerant Corynebacterium variabile HRJ4 and biochar.
The present work aimed to develop a novel strategy to bioremediate the petroleum hydrocarbon contaminants in the environment. Salt tolerant bacterium was isolated from Dagang oilfield, China and identified as Corynebacterium variabile HRJ4 based on 16S rRNA gene sequence analysis. The bacterium had a high salt tolerant capability and biochar was developed as carrier for the bacterium. The bacteria with biochar were most effective in degradation of n-alkanes (C16, C18, C19, C26, C28) and polycyclic aromatic hydrocarbons (NAP, PYR) mixture. The result demonstrated that immobilization of C. variabile HRJ4 with biochar showed higher degradation of total petroleum hydrocarbons (THPs) up to 78.9% after 7-day of incubation as compared to the free leaving bacteria. The approach of this study will be helpful in clean-up of petroleum-contamination in the environments through bioremediation process using eco-friendly and cost effective materials like biochar.
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address: zhanghairong1017@sina.com.
Hairong
H
Zhang
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China; Tianjin Engineering Center of Environmental Diagnosis and Contamination Remediation, Tianjin 300071, China; Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin 300071, China. Electronic address: tangjch@nankai.edu.cn.
Jingchun
J
Tang
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
Lin
L
Wang
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
Juncheng
J
Liu
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
Ranjit Gajanan
RG
Gurav
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
Kejing
K
Sun
D001673:Biodegradation, Environmental; D002606:Charcoal / Q000737:chemistry*; D002681:China; D003352:Corynebacterium / Q000502:physiology*; D010578:Petroleum / Q000032:analysis / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D055049:Salt Tolerance; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; C540010:biochar; D002606:Charcoal
Biochar; Bioremediation; Corynebacterium variabile HRJ4; Immobilization; Petroleum hydrocarbons