Metal-doped inorganic nanoparticles for multiplex detection of biomarkers by a sandwich-type ICP-MS immunoassay.
Metal-doped inorganic nanoparticles were synthesized for the multiplex detection of biomarkers by a sandwich-type inductively coupled plasma mass spectrometry (ICP-MS) immunoassay. The synthesized Cs-doped multicore magnetic nanoparticles (MMNPs) were used not only for magnetic extraction of targets but also for ratiometric measurement in ICP-MS. In addition, three different metal/dye-doped silica nanoparticles (SNPs) were synthesized as probes for multiplex detection: Y/RhBITC (rhodamine B isothiocyanate)-doped SNPs for CRP (cardiovascular disease), Cd/RhBITC-doped SNPs for AFP (tumor), and Au/5(6)-XRITC (X-rhodamine-5-(and-6)-isothiocyanate)-doped SNPs for NSE (heart disease). For quantification, the doped metals of SNPs were measured by ICP-MS and then the signal ratio to Cs of MMNPs was plotted with respect to the concentration of targets by a ratiometry. Limits of detection (LOD) of 0.35 ng/mL to 77 ng mL(-1) and recoveries of 83%-125% were obtained for serum samples spiked with the biomarkers. Since no sample treatment was necessary prior to the extraction, the proposed method provided short analysis time and convenience for the multiplex determination of biomarkers, which will be valuable for clinical application.
Future Environmental Research Center, Korea Institute of Toxicology, Jinju, 660-844, South Korea; Department of Chemistry, Dankook University, Yongin-si, Gyeonggi-do, 448-701, South Korea.
Jung Aa
JA
Ko
Department of Chemistry, Dankook University, Yongin-si, Gyeonggi-do, 448-701, South Korea. Electronic address: plasma@dankook.ac.kr.
H B
HB
Lim
D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis; D002104:Cadmium / Q000737:chemistry*; D003429:Cross Reactions; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
D015415:Biomarkers; D002104:Cadmium; D012822:Silicon Dioxide
Biomarkers; ICP-MS; Immunoassay; Metal-doped magnetic nanoparticles; Multiplex detection