A Novel Skin Splint for Accurately Mapping Dermal Remodeling and Epithelialization During Wound Healing.
The mouse excisional dorsal full-thickness wound model with a silicon splint fixed on the skin has been widely used to mimic human wound healing. However, the method cannot accurately quantify dermal remodeling, since the initial point of epithelialization on the wound surface is unclear. To overcome this limitation, we have developed a novel mouse excisional wound model to assess the degree of epithelial extension and regeneration, using a plastic ring-shaped splint fixed beneath the surrounding epidermal tissue. At the end of the experiment, tissue samples were fixed in formalin, the splint was excised, and paraffin sections were prepared. Splint holes, corresponding to the prior location of the splint, were evident on the tissue cross-sections, and the epidermis above the holes was considered the initial excision site. The epidermal contraction and epithelial regeneration, as independent essential tissue alterations in wound healing, could be distinguishable and quantified. Compared with previous splint models, this method provides an accurate evaluation of epidermal processes in wound healing, and can be a platform to assess the effects of various wound healing factors. J. Cell. Physiol. 232: 1225-1232, 2017. © 2016 Wiley Periodicals, Inc.
Central Laboratory for Pathology and Morphology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
Shiro
S
Jimi
Multidisciplinary Department of Medical-Surgical and Dental Specialties, Second University of Naples, Naples, Italy.
Francesco
F
De Francesco
Multidisciplinary Department of Medical-Surgical and Dental Specialties, Second University of Naples, Naples, Italy.
Giuseppe A
GA
Ferraro
Department of Reconstructive Plastic Surgery-Hand Surgery, AOU "Ospedali Riuniti", Ancona, Italy.
Michele
M
Riccio
Department of Pharmaceutical Science, Fukuoka University, Fukuoka, Japan.
Shuuji
S
Hara
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D020405:Dermis / Q000473:pathology*; D006943:Hyperglycemia / Q000097:blood / Q000150:complications / Q000473:pathology; D053553:Keratin-6 / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000097:blood / Q000150:complications / Q000473:pathology; D062326:Re-Epithelialization; D013165:Splints; D013997:Time Factors
D001786:Blood Glucose; D053553:Keratin-6