Immobilized MAS1 lipase showed high esterification activity in the production of triacylglycerols with n-3 polyunsaturated fatty acids.
Immobilization of lipase MAS1 from marine Streptomyces sp. strain W007 and its application in catalyzing esterification of n-3 polyunsaturated fatty acids (PUFA) with glycerol were investigated. The resin XAD1180 was selected as a suitable support for the immobilization of lipase MAS1, and its absorption ability was 75mg/g (lipase/resin ratio) with initial buffer pH value of 8.0. The thermal stability of immobilized MAS1 was improved significantly compared with that of the free lipase. Immobilized MAS1 had no regiospecificity in the hydrolysis of triolein. The highest esterification degree (99.31%) and TAG content (92.26%) by immobilized MAS1-catalyzed esterification were achieved under the optimized conditions, which were significantly better than those (82.16% and 47.26%, respectively) by Novozym 435. More than 92% n-3 PUFA was incorporated into TAG that had similar fatty acids composition to the substrate (n-3 PUFA). The immobilized MAS1 exhibited 50% of its initial activity after being used for five cycles.
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
Xiumei
X
Wang
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Daoming
D
Li
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
Man
M
Qu
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
Rabia
R
Durrani
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
Bo
B
Yang
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address: yonghw@scut.edu.cn.
Yonghua
Y
Wang
D004800:Enzymes, Immobilized / Q000032:analysis* / Q000378:metabolism; D004951:Esterification / Q000502:physiology; D015525:Fatty Acids, Omega-3 / Q000096:biosynthesis / Q000737:chemistry*; D006868:Hydrolysis; D008049:Lipase / Q000032:analysis* / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis / Q000737:chemistry*
D004800:Enzymes, Immobilized; D015525:Fatty Acids, Omega-3; D014280:Triglycerides; D008049:Lipase
Esterification; Immobilization; Lipase; Triacylglycerols; n-3 polyunsaturated fatty acids