Discriminating Bio-aerosols from Non-Bio-aerosols in Real-Time by Pump-Probe Spectroscopy.
The optical identification of bioaerosols in the atmosphere and its discrimination against combustion related particles is a major issue for real-time, field compatible instruments. In the present paper, we show that by embedding advanced pump-probe depletion spectroscopy schemes in a portable instrument, it is possible to discriminate amino acid containing airborne particles (bacteria, humic particles, etc.) from poly-cyclic aromatic hydrocarbon containing combustion particles (Diesel droplets, soot, vehicle exhausts) with high selectivity. Our real-time, multi-modal device provides, in addition to the pump-probe depletion information, fluorescence spectra (over 32 channels), fluorescence lifetime and Mie scattering patterns of each individually flowing particle in the probed air.
Université de Genève, GAP-Biophotonics, 22 chemin de Pinchat, Carouge, 1211 Geneva 4, Switzerland.
Gustavo
G
Sousa
Université de Genève, GAP-Biophotonics, 22 chemin de Pinchat, Carouge, 1211 Geneva 4, Switzerland.
Geoffrey
G
Gaulier
Université de Genève, GAP-Biophotonics, 22 chemin de Pinchat, Carouge, 1211 Geneva 4, Switzerland.
Luigi
L
Bonacina
Université de Genève, GAP-Biophotonics, 22 chemin de Pinchat, Carouge, 1211 Geneva 4, Switzerland.
Jean-Pierre
JP
Wolf