Rufibacter quisquiliarum sp. nov., a new member of the phylum Bacteroidetes isolated from a bioreactor treating landfill leachate.
A novel bacterium, CAI-18bT, was isolated from a bioreactor that treated landfill leachate using an oligotrophic growth medium. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CAI-18bT is a member of the genus Rufibacter, showing 97.1 % pairwise similarity to Rufibacter roseus H359T, 96.4 % to Rufibacter tibetensis 1351T, 96.4 % to Rufibacter glacialis MDT1-10-3T and 96.0 % to Rufibacter immobilis MCC P1T. Strain CAI-18bT was rod-shaped, motile, oxidase- and catalase-positive. The predominant fatty acids were iso-C15 : 0 (24.1 %) and iso-C17 : 1 I (22.3 %), the major respiratory quinone was MK-7, and the predominant polar lipids were phosphatidylethanolamine and an unknown aminophospholipid. The G+C content of the genomic DNA of strain CAI-18bT was 50.7 mol%. The novel bacterium can be distinguished from related type strains based on its ability to assimilate N-acetylglucosamine and gentiobiose. On the basis of the phenotypic, chemotaxonomic and molecular data, strain CAI-18bT represents a novel species of the genus Rufibacter, for which the name Rufibacter quisquiliarum sp. nov. is proposed. The type strain is CAI-18bT (=DSM 29854T=NCAIM B.02614T).
Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, 1117 Budapest, Hungary.|Department of Bioengineering, Sapientia Hungarian University of Transylvania, Piaţa Libertăţii 1, 530104 Miercurea Ciuc, Romania.
Tamás
T
Felföldi
Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, 1117 Budapest, Hungary.
Anikó
A
Mentes
Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Peter
P
Schumann
Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, 1117 Budapest, Hungary.
Zsuzsa
Z
Kéki
Department of Bioengineering, Sapientia Hungarian University of Transylvania, Piaţa Libertăţii 1, 530104 Miercurea Ciuc, Romania.
István
I
Máthé
Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, 1117 Budapest, Hungary.
Károly
K
Márialigeti
Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, 1117 Budapest, Hungary.
Erika M
EM
Tóth
D015373:Bacterial Typing Techniques; D001482:Base Composition; D019149:Bioreactors / Q000382:microbiology*; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012383:Romania; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014874:Water Pollutants, Chemical
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7