A Conserved Role for Girdin in Basal Body Positioning and Ciliogenesis.
Primary cilia are ubiquitous sensory organelles that mediate diverse signaling pathways. Cilia position on the cell surface is determined by the location of the basal body (BB) that templates the cilium. The mechanisms that regulate BB positioning in the context of ciliogenesis are largely unknown. Here we show that the conserved signaling and scaffolding protein Girdin localizes to the proximal regions of centrioles and regulates BB positioning and ciliogenesis in Caenorhabditis elegans sensory neurons and human RPE-1 cells. Girdin depletion alters localization of the intercentriolar linker and ciliary rootlet component rootletin, and rootletin knockdown in RPE-1 cells mimics Girdin-dependent phenotypes. C. elegans Girdin also regulates localization of the apical junction component AJM-1, suggesting that in nematodes Girdin may position BBs via rootletin- and AJM-1-dependent anchoring to the cytoskeleton and plasma membrane, respectively. Together, our results describe a conserved role for Girdin in BB positioning and ciliogenesis.
Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, MA 02454, USA. Electronic address: ivn@brandeis.edu.
Inna V
IV
Nechipurenko
Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, MA 02454, USA.
Anique
A
Olivier-Mason
Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, MA 02454, USA.
Anna
A
Kazatskaya
School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland.
Julie
J
Kennedy
Department of Genetics, Harvard Medical School and Boston Children's Hospital, Boston, MA 02115, USA.
Ian G
IG
McLachlan
Department of Genetics, Harvard Medical School and Boston Children's Hospital, Boston, MA 02115, USA.
Maxwell G
MG
Heiman
School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland.
Oliver E
OE
Blacque
Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, MA 02454, USA. Electronic address: sengupta@brandeis.edu.
Piali
P
Sengupta
D000818:Animals; D064652:Basal Bodies / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development; D029742:Caenorhabditis elegans Proteins / Q000096:biosynthesis / Q000235:genetics*; D002502:Centrioles / Q000235:genetics; D002923:Cilia / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000235:genetics*; D003599:Cytoskeleton / Q000235:genetics; D006801:Humans; D008840:Microfilament Proteins / Q000096:biosynthesis / Q000235:genetics*; D008870:Microtubules / Q000235:genetics; D009024:Morphogenesis / Q000235:genetics*; D015388:Organelles / Q000235:genetics; D011984:Sensory Receptor Cells / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D033921:Vesicular Transport Proteins / Q000096:biosynthesis / Q000235:genetics*
C441580:AJM-1 protein, C elegans; C502451:CCDC88A protein, human; C499237:CROCC protein, human; D029742:Caenorhabditis elegans Proteins; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D033921:Vesicular Transport Proteins