Evaluating cytotoxic effect of nanoliposomes encapsulated with umbelliprenin on 4T1 cell line.
Cytotoxicity of umbelliprenin has been found in various cancer cell lines such as, prostate, breast, CLL, and skin. Encapsulating chemotherapeutic agents with nanoliposomes have been resulted in improved cytotoxicity effects than their free forms. However, whether nanoliposomal form of umbelliprenin could have higher cytotoxic effect than free umbelliprenin is not clarified yet. After synthesizing umbelliprenin, different concentrations (3, 6, 12, 25, 50, 100, 200 μg/ml) applied on the mouse mammary carcinoma cell line (4T1) for 24, 48, and 72 h at 37°C. Afterwards, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was performed to analyze cytotoxicity. MTT assay results showed that IC50of umbelliprenin in dimethyl sulfoxide (DMSO) (30.92, 30.64, and 62.23 for 24, 48, 72 h incubation, respectively) decreased (5.8, 5.0, 3.5 for 24, 48, 72 h incubation, respectively) when encapsulated with nanoliposomes. Nanoliposomal umbelliprenin cytotoxicity affected cell viability in concentration and time-dependent manner. Our study recommended nanoliposomal umbelliprenin as the most effective chemotherapeutic agent against the mouse mammary carcinoma cell line viability. Future in vivo studies and clinical trials are needed.
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohsen
M
Rashidi
School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Alireza
A
Ahmadzadeh
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Seyed Ali
SA
Ziai
Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mahsa
M
Narenji
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Jamshidik@gmail.com.|Department of Pharmacology, Faculty of Medicine, Shaheed Beheshti University of Medical Sciences, Velenjac Street, Tehran, Iran. Jamshidik@gmail.com.
Hamidreza
H
Jamshidi
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D020128:Inhibitory Concentration 50; D008081:Liposomes / Q000633:toxicity*; D051379:Mice; D053758:Nanoparticles / Q000633:toxicity*; D014468:Umbelliferones / Q000737:chemistry / Q000494:pharmacology*
D008081:Liposomes; D014468:Umbelliferones; C003744:umbelliprenin
Cancer; Nanoliposomes; Umbelliprenin