A comparative analysis of chemical compositions in Camellia sinensis var. puanensis Kurihara, a novel Chinese tea, by HPLC and UFLC-Q-TOF-MS/MS.
Camellia sinensis var. puanensis Kurihara (Puan tea) is a kind of ancient tea plant newly found in Jiangxipo and the surrounding areas of Puan County (Guizhou, China). People there always believe that drinking Puan tea is beneficial to the promotion of health and prevention of diseases. However, detailed information on its compositions has not been reported. Therefore, in this study, the varieties and contents of purine alkaloids and polyphenols in Puan tea were identified and determined by HPLC and UFLC-Q-TOF-MS/MS. Our results showed that theacrine, but not caffeine, was the dominated purine alkaloid detected in Puan tea. Meanwhile, Puan tea contained B-type procyanidin dimer, trimer and dimer monogallate, which were not detected in Camellia sinensis, Camellia ptilophylla and Camellia assamica var. kucha. The obtained results could support the local uses of Puan tea in health and nutrition and contribute to the research of tea variety.
Anti-Stress and Health Research Center, College of Pharmacy, Jinan University, Guangzhou 510632, China; Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China.
Yi-Fang
YF
Li
Anti-Stress and Health Research Center, College of Pharmacy, Jinan University, Guangzhou 510632, China; Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China.
Shu-Hua
SH
Ouyang
Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China.
Yi-Qun
YQ
Chang
Anti-Stress and Health Research Center, College of Pharmacy, Jinan University, Guangzhou 510632, China; Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China.
Ting-Mei
TM
Wang
College of Traditional Chinese Materia Medica, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.
Wei-Xi
WX
Li
Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China.
Hai-Yan
HY
Tian
Hongxin Tea Industry Development Co., LTD, Puan 561500, China.
Hong
H
Cao
Anti-Stress and Health Research Center, College of Pharmacy, Jinan University, Guangzhou 510632, China; Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China.
Hiroshi
H
Kurihara
Anti-Stress and Health Research Center, College of Pharmacy, Jinan University, Guangzhou 510632, China; Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China. Electronic address: rongronghe@jnu.edu.cn.
Rong-Rong
RR
He
D000470:Alkaloids / Q000032:analysis; D002110:Caffeine / Q000032:analysis; D028241:Camellia sinensis / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D006801:Humans; D018515:Plant Leaves; D059808:Polyphenols / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards; D013662:Tea / Q000737:chemistry*; D014527:Uric Acid / Q000031:analogs & derivatives* / Q000032:analysis
D000470:Alkaloids; D059808:Polyphenols; D013662:Tea; D014527:Uric Acid; D002110:Caffeine; C077059:1,3,7,9-tetramethyluric acid
Camellia sinensis var. puanensis Kurihara; HPLC; Polyphenols; Proanthocyanidins; Purine alkaloids; Theacrine; UFLC-Q-TOF-MS/MS