Ceramic scaffolds enriched with gentamicin loaded poly(lactide-co-glycolide) microparticles for prevention and treatment of bone tissue infections.
Bone scaffolds are susceptible for bacterial infection when implanted, particularly in compromised bone. Therefore anti-bacterial bone scaffolds are desirable. Here a novel approach to provide bactericidal properties for titanium dioxide scaffolds is proposed. Gentamicin loaded poly(L-lactide-co-glycolide) microparticles were immobilized on the scaffold pore walls by sodium alginate hydrogel. The results show that the microparticles were effectively immobilized on the scaffolds. Desired burst release was observed within the first 8h and gentamicin dose reached 125μg from single scaffold that corresponded to ~25% of total drug introduced in the system. Following the initial burst, the dose was gradually decreasing up to day 10 and afterwards a sustained release of 3μg/day was measured. Cumulatively ~90% of the drug was delivered up to day 50. Above pattern, i.e. burst release with following sustained release, is desired for prevention of perioperative bone infections: burst release stops local infections during post-implantation "decisive period" while further sustained drug release prevents bacterial recolonization. In vitro studies confirmed antimicrobial activity of released gentamicin against Staphylococcus spp. and cytocompatibility of the system with osteoblast-like cells (MG-63). Thus the system is a viable option for the treatment of bone tissue defects.
AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, Al. A. Mickiewicza 30, 30-059 Krakow, Poland.
Łucja
Ł
Rumian
University of Oslo, Institute for Clinical Dentistry, Department of Biomaterials, Geitmyrsveien 71, P.O. Box 1109, Blindern, NO-0317 Oslo, Norway.
Hanna
H
Tiainen
AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, Al. A. Mickiewicza 30, 30-059 Krakow, Poland.
Urszula
U
Cibor
AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, Al. A. Mickiewicza 30, 30-059 Krakow, Poland.
Małgorzata
M
Krok-Borkowicz
Jagiellonian University, Medical College, Department of Microbiology, ul. Czysta 18, 31-121 Krakow, Poland.
Monika
M
Brzychczy-Włoch
University of Oslo, Institute for Clinical Dentistry, Department of Biomaterials, Geitmyrsveien 71, P.O. Box 1109, Blindern, NO-0317 Oslo, Norway.
Håvard J
HJ
Haugen
AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, Al. A. Mickiewicza 30, 30-059 Krakow, Poland. Electronic address: epamula@agh.edu.pl.
Elżbieta
E
Pamuła
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology*; D002460:Cell Line; D002516:Ceramics / Q000737:chemistry*; D065546:Drug Liberation; D005839:Gentamicins / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D008863:Microspheres; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010019:Osteomyelitis / Q000188:drug therapy* / Q000517:prevention & control*; D010316:Particle Size; D011098:Polyglactin 910; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry
D000900:Anti-Bacterial Agents; D005839:Gentamicins; C009495:titanium dioxide; D011098:Polyglactin 910; D014025:Titanium
Bone infections; Gentamicin; Microparticles; PLGA; Titanium dioxide scaffolds