In Vitro Cytotoxicity of Maxillofacial Silicone Elastomers: Effect of Nano-particles.
OBJECTIVE
Silicone elastomers are generally used for maxillofacial extraoral prostheses. The purpose of this in vitro study was to evaluate the cytotoxicity of different kinds of nanoparticles added to two types of maxillofacial elastomers.


METHODS
A-2000 and A-2006 silicone elastomers were used. The silicone specimens were divided into eight groups according to the presence of additional nanoparticles (n = 18). The following represents the groups in the study: Group A: A-2000 silicone (control group); Group B: A-2006 silicone (control group); Group C: A-2000 silicone and the addition of titanium dioxide (TiO2); Group D: A-2006 silicone and the addition of TiO2; Group E: A-2000 silicone and the addition of fumed silica; Group F: A-2006 silicone and the addition of fumed silica; Group G: A-2000 silicone and the addition of silaned silica; Group H: A-2006 silicone and the addition of silaned silica. A paired sample t-test was used to analyze the cytotoxicity of each group after 24, 48, and 72 hours.


RESULTS
Based on the results of the 24-hour analysis, the biocompatibility values of the (A-2006) fumed silica group were higher than those of the control groups. There was no statistically significant difference in A-2006 and A-2000 groups. The cytotoxicity values of the control groups and TiO2(A-2000 silicone) elastomer groups increased at all test times; however, the cytotoxicity values of the TiO2(A-2006), fumed silica (A-2006), silaned silica (A-2006), fumed silica (A-2000), and silaned silica (A-2000) groups increased significantly only from 24 to 48 hours.


CONCLUSIONS
Nanoparticles of TiO2, fumed silica, and silaned silica added to a commercial silicone-based elastomer used for fabrication of maxillofacial prostheses are nontoxic.
Department of Prosthodontics, University of Osmangazi, Faculty of Dentistry, Eskişehir, Turkey.
Canan
C
Akay
Department of Prosthodontics, University of Gazi, Faculty of Dentistry, Ankara, Turkey.
Pinar
P
Cevik
Department of Prosthodontics, University of Gazi, Faculty of Dentistry, Ankara, Turkey.
Duygu
D
Karakis
Department of Biology, University of Hacettepe, Faculty of Science, Ankara, Turkey.
Handan
H
Sevim
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D003603:Cytotoxins / Q000009:adverse effects; D005347:Fibroblasts / Q000187:drug effects; D066298:In Vitro Techniques; D008447:Maxillofacial Prosthesis / Q000009:adverse effects*; D051379:Mice; D053758:Nanoparticles / Q000009:adverse effects*; D012826:Silicone Elastomers / Q000009:adverse effects*
C587764:A-2006 elastomer; D003603:Cytotoxins; D012826:Silicone Elastomers
Cell culture; MTT test; maxillofacial silicone elastomer