Preloading with L-BPA, L-tyrosine and L-DOPA enhances the uptake of [18F]FBPA in human and mouse tumour cell lines.
Aim of this study was to investigate if cellular [18F]FBPA uptake can be increased upon preloading with amino acids. [18F]FBPA uptake was assessed in HuH-7, CaCo-2 and B16-F1 cells pretreated with different concentrations or incubation times of L-BPA, L-tyrosine or L-DOPA. Without preloading, highest uptake of [18F]FBPA was observed in B16-F1 cells, followed by CaCo-2 cells and HuH-7 cells. In all cell lines higher [18F]FBPA accumulation (up to 1.65-fold) was obtained with increasing L-BPA, L-DOPA and L-tyrosine concentrations.
Ludwig Boltzmann Institute for Cancer Research, Vienna, Austria.
Bettina
B
Wingelhofer
Biomedical Systems, Health & Environment Department, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria.
Katharina
K
Kreis
Biomedical Systems, Health & Environment Department, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria.
Severin
S
Mairinger
Biomedical Systems, Health & Environment Department, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria.
Viktoria
V
Muchitsch
Biomedical Systems, Health & Environment Department, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria.
Johann
J
Stanek
Biomedical Systems, Health & Environment Department, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria.
Thomas
T
Wanek
Biomedical Systems, Health & Environment Department, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria; Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria.
Oliver
O
Langer
Biomedical Systems, Health & Environment Department, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria. Electronic address: claudia.kuntner@ait.ac.at.
Claudia
C
Kuntner
D000818:Animals; D001896:Boron Compounds / Q000008:administration & dosage*; D016754:Boron Neutron Capture Therapy / Q000379:methods*; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004357:Drug Synergism; D006801:Humans; D007980:Levodopa / Q000008:administration & dosage*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism*; D010649:Phenylalanine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D011422:Propionates / Q000493:pharmacokinetics*; D014443:Tyrosine / Q000008:administration & dosage*; D015854:Up-Regulation / Q000187:drug effects
D001896:Boron Compounds; D004338:Drug Combinations; D011422:Propionates; C080346:beta-(4-fluorobenzoyl)propionic acid; D014443:Tyrosine; D007980:Levodopa; D010649:Phenylalanine; C033685:4-boronophenylalanine
Amino acid transporters; BNCT; Preloading; System L; [(18)F]FBPA