Can ultraviolet light C decrease the environmental burden of antimicrobial-resistant and -sensitive bacteria on textiles?
BACKGROUND
The environment is important in transmission of bacteria. Textiles are difficult and time consuming to clean; ultraviolet light C (UVC) is germicidal and may be an effective disinfection method for textile surfaces.


OBJECTIVE
This study evaluated the efficacy of UVC, a commercial quaternary ammonium compound antimicrobial spray (FAS) and UVC+FAS combined for reducing bacterial colonization on experimentally contaminated textiles.


METHODS
Microfibre, cotton and polyester were inoculated with meticillin-sensitive and -resistant Staphylococcus aureus (MSSA and MRSA), Staphylococcus pseudintermedius (MSSP and MRSP), Pseudomonas aeruginosa or Escherichia coli. ATCC®strains were used except for MRSP, for which ten canine clinical isolates were collected. Textiles were treated with three doses of UVC (13 mJ/cm2, 54 mJ/cm2or 270 mJ/cm2), FAS or both (FAS and UVC at 270 mJ/cm2). UVC was delivered using a modified mercury-based lamp. Bleach (8.25%) was used as a positive control. Negative controls received no treatment. Surface bacterial counts were determined 24 h post-treatment.


RESULTS
The lower dosages (13 mJ/cm2and 54 mJ/cm2) of UVC had >90% colony forming unit (CFU) reduction, 270 mJ/cm2had >99% CFU reduction and combined UVC+FAS had 100% CFU reduction against all bacterial strains on all surfaces (P < 0.05). Ten experiments showed that treatment with UVC had a greater CFU reduction when compared to FAS alone (P < 0.05). A majority of those experiments (seven of 10) involved Gram-negative species (P. aeruginosa or E. coli).


CONCLUSIONS
UVC quickly reduced the bacterial burden on textiles to greater than 90%; UVC may be a better disinfecting agent than FAS for Gram-negative species.
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2089 SW 16th Avenue, Gainesville, FL, 32608, USA.
Jennifer J
JJ
Bentley
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2089 SW 16th Avenue, Gainesville, FL, 32608, USA.
Domenico
D
Santoro
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2089 SW 16th Avenue, Gainesville, FL, 32608, USA.
Dunbar W
DW
Gram
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2089 SW 16th Avenue, Gainesville, FL, 32608, USA.
Mauricio
M
Dujowich
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, 2089 SW 16th Avenue, Gainesville, FL, 32608, USA.
Rosanna
R
Marsella
D001419:Bacteria / Q000528:radiation effects*; D032561:Cotton Fiber; D004203:Disinfection; D011091:Polyesters; D013784:Textiles / Q000382:microbiology*; D014466:Ultraviolet Rays
D011091:Polyesters