Lipoprotein profiles of hepatic cell lines at various stages of differentiation.
We studied the lipoprotein profiles of human hepatic cells at various stages of differentiation. The production of three major classes of lipoproteins, very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), and high-density lipoprotein (HDL), was detected in three well-differentiated human hepatoma cell lines and primary human hepatocytes; however, these lipoproteins were not detected in the culture medium in which undifferentiated hepatoma cell lines were grown. Reverse transcription polymerase chain reaction analysis demonstrated that the expression levels of apolipoprotein A1 (ApoA1), ApoB100, and microsomal triglyceride transfer protein (MTP) were markedly lower in the undifferentiated hepatoma cell lines than in the well-differentiated hepatoma cell lines and primary hepatocytes. These results indicate that apolipoprotein synthesis, and triglyceride-transport by MTP might be rate-limiting steps in lipoprotein production in mature hepatic cells.
Akita Research Institute of Food and Brewing, 4-26, Sanuki, Arayamachi, Akita, 010-1623, Japan. asasaki@arif.pref.akita.jp.|Graduate School of Bioresource Science, Akita Prefectural University, 241-438, Shimoshinjoh, Nakano, Akita, 010-0195, Japan. asasaki@arif.pref.akita.jp.
Akira
A
Sasaki
Skylight Biotech, 100-4, Sunada, Iijima, Akita, 010-0911, Japan.
Fumiko
F
Kimura
Skylight Biotech, 100-4, Sunada, Iijima, Akita, 010-0911, Japan.
Mizuho
M
Miura
Skylight Biotech, 100-4, Sunada, Iijima, Akita, 010-0911, Japan.
Gen
G
Toshima
Skylight Biotech, 100-4, Sunada, Iijima, Akita, 010-0911, Japan.
Junichiro
J
Takahashi
ORIENTAL YEAST Co., Ltd., 3-6-10, Azusawa, Itabashi-ku, Tokyo, 174-8505, Japan.
Sayuri
S
Maruya
Graduate School of Bioresource Science, Akita Prefectural University, 241-438, Shimoshinjoh, Nakano, Akita, 010-0195, Japan.
Masayuki
M
Kobayashi
Akita Research Institute of Food and Brewing, 4-26, Sanuki, Arayamachi, Akita, 010-1623, Japan.
Keishi
K
Hata
D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000378:metabolism; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
D008074:Lipoproteins; D014280:Triglycerides; D002784:Cholesterol
Apolipoprotein; Hepatic cell differentiation; Lipoprotein