Smelling the difference: Transcriptome, proteome and volatilome changes after mating.
Mushrooms, such as Schizophyllum commune, have a specific odor. Whether this is linked to mating, prerequisite for mushroom formation, or also found in monokaryotic, unmated strains, was investigated with a comprehensive study on the transcriptome and proteome of this model organism. Mating interactions were investigated using a complete, cytosolic proteome map for unmated, monokaryotic, as well as for mated, dikaryotic mycelia. The regulations of the proteome were compared to transcriptional changes upon mating and to changes in smell by volatilome studies. We could show a good overlap between proteome and transcriptome data, but extensive posttranslational regulation was identified for more than 80% of transcripts. This suggests down-stream regulation upon interaction of mating partners and formation of the dikaryon that is competent to form fruiting bodies. The volatilome was shown to respond to mating by a broader spectrum of volatiles and increased emission of the mushroom smell molecules 3-octanone and 1-octen-3-ol, as well as ethanol and β-bisabolol in the dikaryon. Putatively involved biosynthetic proteins like alcohol dehydrogenases, Ppo-like oxygenases, or sesquiterpene synthases showed correlating transcriptional regulation depending on either mono- or dikaryotic stages.
Friedrich Schiller University, Institute of Microbiology, Microbial Communication, Neugasse 25, 07743 Jena, Germany.
Daniela
D
Freihorst
Friedrich Schiller University, Institute of Microbiology, Microbial Communication, Neugasse 25, 07743 Jena, Germany.
Melanie
M
Brunsch
Friedrich Schiller University, Institute of Microbiology, Microbial Communication, Neugasse 25, 07743 Jena, Germany.
Sophia
S
Wirth
Friedrich Schiller University, Institute of Microbiology, Microbial Communication, Neugasse 25, 07743 Jena, Germany.
Katrin
K
Krause
Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Adolf-Reichwein-Straße 23, 07745 Jena, Germany.
Olaf
O
Kniemeyer
Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Adolf-Reichwein-Straße 23, 07745 Jena, Germany.
Jörg
J
Linde
Max Planck Institute for Chemical Ecology, Department of Bioorganic Chemistry, Hans-Knöll-Straße 8, 07745 Jena, Germany.
Maritta
M
Kunert
Max Planck Institute for Chemical Ecology, Department of Bioorganic Chemistry, Hans-Knöll-Straße 8, 07745 Jena, Germany.
Wilhelm
W
Boland
Friedrich Schiller University, Institute of Microbiology, Microbial Communication, Neugasse 25, 07743 Jena, Germany. Electronic address: erika.kothe@uni-jene.de.
Erika
E
Kothe
D020869:Gene Expression Profiling; D055432:Metabolomics; D056265:Microbial Interactions; D020543:Proteome / Q000032:analysis*; D011995:Recombination, Genetic; D012567:Schizophyllum / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000378:metabolism*
D020543:Proteome; D055549:Volatile Organic Compounds
Basidiomycete; Mating; Proteome; Schizophyllum commune; Transcriptome; Volatilome