(1)H-NMR based metabolomics study for the detection of the human urine metabolic profile effects of Origanum dictamnus tea ingestion.
(1)H NMR spectroscopy was employed to investigate the repercussion of Origanum dictamnus tea ingestion in several volunteers' urine metabolic profiles, among them two with chronic inflammatory bowel diseases (IBD), mild IBD and Crohn's disease. Herein, we demonstrate that the concentrations of a lot of urinary metabolites such as hippurate, trimethylamine oxide (TMAO), citrate, and creatinine are altered, which prompts the intestinal microflora function/content perturbation as well as kidney function regulation by dictamnus tea. Interestingly, our preliminary results showed that a high dose of dictamnus tea intake appeared to be toxic for a person with Crohn's disease, since it caused high endogenous ethanol excretion in urine. All subjects' metabolic effects caused by the dictamnus tea appeared to be reversible, when all volunteers stopped its consumption. Finally, we highlight that individuals' metabolic phenotype is reflected in their urine biofluid before and after the dictamnus tea effect while all individuals have some common and different metabolic responses to this tea, implying that each phenotype has a quite different response to this tea consumption.
Department of Biological Applications and Technology, University of Ioannina, GR-451 10 Ioannina, Greece. ptakis@cc.uoi.gr atrogani@uoi.gr.
Panteleimon G
PG
Takis
Mariam-Eleni
ME
Oraiopoulou
Constantinos
C
Konidaris
Anastassios N
AN
Troganis
D000328:Adult; D015415:Biomarkers / Q000652:urine; D019343:Citric Acid / Q000652:urine; D003404:Creatinine / Q000652:urine; D003424:Crohn Disease / Q000178:diet therapy* / Q000276:immunology / Q000503:physiopathology / Q000652:urine; D000431:Ethanol / Q000652:urine; D005260:Female; D006115:Greece; D006626:Hippurates / Q000652:urine; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000178:diet therapy* / Q000276:immunology / Q000503:physiopathology / Q000652:urine; D008297:Male; D055432:Metabolomics / Q000379:methods; D008744:Methylamines / Q000652:urine; D019906:Nuclear Magnetic Resonance, Biomolecular; D031341:Origanum / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D025341:Principal Component Analysis; D065667:Renal Elimination; D012720:Severity of Illness Index; D000069459:Teas, Herbal / Q000009:adverse effects* / Q000191:economics
D015415:Biomarkers; D006626:Hippurates; D008744:Methylamines; D000069459:Teas, Herbal; D019343:Citric Acid; D000431:Ethanol; D003404:Creatinine; C005855:trimethyloxamine; C030514:hippuric acid