Peptococcus simiae sp. nov., isolated from rhesus macaque faeces and emended description of the genus Peptococcus.
A study of the faecal microbiome in three healthy female rhesus macaques revealed the presence of a novel obligately anaerobic, chemoorganoheterotrophic, non-sporing, coccoid, non-motile, Gram-stain-positive bacterial species. Three strains of this species, designated as M108T, M916-1/1, and M919-2/1, were non-haemolytic, H2S-positive, catalase-positive, bile- and NaCl-sensitive and required peptone for growth. Strains also were asaccharolytic, able to utilize sulfite, thiosulfate and elemental sulfur as electron acceptors, and produced acetic and butyric acids as metabolic end-products. Strain M108T is characterized by the prevalence of C14 : 0, C16 : 0 and C18 : 1ω9cis dimethyl acetal among the cellular fatty acids, and the presence of MK-10 menaquinone. The DNA G+C content was found to be 51 mol%. Phylogenetic analysis of partial 16S rRNA gene sequences of strains M108T, M916-1/1 and M919-2/1 placed these strains into the genus Peptococcus (family Peptococcaceae). On the basis of phenotypic and genotypic properties we conclude that these strains represent a novel bacterial species for which the name Peptococcus simiae sp. nov. is proposed. The type strain is M108T (=DSM 100347T=VKM B-2932T).
Department of Microbiology and Virology, Pirogov Russian National Research Medical University, Moscow 117997, Russia.
Andrei N
AN
Shkoporov
Department of Microbiology and Virology, Pirogov Russian National Research Medical University, Moscow 117997, Russia.
Boris A
BA
Efimov
Animal Science Department, Biomedical Primate Research Center, P.O. Box 3306, 2280 GH Rijswijk, The Netherlands.
Ivanela
I
Kondova
Animal Science Department, Biomedical Primate Research Center, P.O. Box 3306, 2280 GH Rijswijk, The Netherlands.
Boudewijn
B
Ouwerling
Department of Microbiology and Virology, Pirogov Russian National Research Medical University, Moscow 117997, Russia.
Andrei V
AV
Chaplin
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
Victoria A
VA
Shcherbakova
Animal Science Department, Biomedical Primate Research Center, P.O. Box 3306, 2280 GH Rijswijk, The Netherlands.
Jan A M
JAM
Langermans
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005243:Feces / Q000382:microbiology; D005260:Female; D008253:Macaca mulatta / Q000382:microbiology*; D010460:Peptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10