Chronic Moderate Alcohol Intakes Accelerate SR-B1 Mediated Reverse Cholesterol Transport.
Cholesterol is essential for all animal life. However, a high level of cholesterol in the body is strongly associated with the progression of various severe diseases. In our study, the potential involvement of alcohol in the regulation of high density lipoprotein (HDL) receptor scavenger receptor class B and type I (SR-B1)-mediated reverse cholesterol transport was investigated. We separated male C57BL/6 mice into four diets: control, alcohol, Control + HC and alcohol + HC. The SR-B1 level and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate- high- density lipoprotein (DiI-HDL) uptake were also measured in AML12 cells and HL7702 cells treated with alcohol. The control + HC diet led to increased hepatic triglyceride and cholesterol levels while alcohol + HC led no significant change. Compared with that of the control group, the SR-B1 mRNA level was elevated by 27.1% (P < 0.05), 123.8% (P < 0.001) and 343.6% (P < 0.001) in the alcohol, control + HC and alcohol + HC groups, respectively. In AML12 and HL7702 cells, SR-B1 level and DiI-HDL uptake were repressed by SR-B1 siRNA or GW9662. However, these effects were reversed through alcohol treatment. These data suggest that a moderate amount of alcohol plays a novel role in reverse cholesterol transport, mainly mediated by PPARγ and SR-B1.
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.|Translational Medicine Center of Northern China, Harbin, China.|Patent Examination Cooperation Center of the Patent Office, SIPO, Sichuan, China.
Menghua
M
Li
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.|Translational Medicine Center of Northern China, Harbin, China.
Yan
Y
Diao
Department of Gastroenterology, Heilongjiang Province Hospital, Harbin, China.
Ying
Y
Liu
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.|Translational Medicine Center of Northern China, Harbin, China.
Hui
H
Huang
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.|Translational Medicine Center of Northern China, Harbin, China.
Yanze
Y
Li
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.|Translational Medicine Center of Northern China, Harbin, China.
Peizhu
P
Tan
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.|Translational Medicine Center of Northern China, Harbin, China.|Department of Clinical Laboratory, Harbin Medical University Cancer Hospital, Harbin, China.
Huan
H
Liang
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.|Translational Medicine Center of Northern China, Harbin, China.
Qi
Q
He
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.|Translational Medicine Center of Northern China, Harbin, China.
Junhui
J
Nie
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.|Translational Medicine Center of Northern China, Harbin, China.
Xingli
X
Dong
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.|Translational Medicine Center of Northern China, Harbin, China.
Yang
Y
Wang
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.|Translational Medicine Center of Northern China, Harbin, China.
Lingyun
L
Zhou
Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.|Translational Medicine Center of Northern China, Harbin, China.
Xu
X
Gao
D000428:Alcohol Drinking / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051122:Scavenger Receptors, Class B / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
D008075:Lipoproteins, HDL; D047495:PPAR gamma; D012333:RNA, Messenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; D014280:Triglycerides; D002784:Cholesterol