Evaluation of mechanical and morphologic features of PLLA membranes as supports for perfusion cells culture systems.
Porous biodegradable PLLA membranes, which can be used as supports for perfusion cell culture systems were designed, developed and characterized. PLLA membranes were prepared via diffusion induced phase separation (DIPS). A glass slab was coated with a binary PLLA-dioxane solution (8wt.% PLLA) via dip coating, then pool immersed in two subsequent coagulation baths, and finally dried in a humidity-controlled environment. Surface and mechanical properties were evaluated by measuring pore size, porosity via scanning electron microscopy, storage modulus, loss modulus and loss angle by using a dynamic mechanical analysis (DMA). Cell adhesion assays on different membrane surfaces were also performed by using a standard count method. Results provide new insights into the foaming methods for producing polymeric membranes and supply indications on how to optimise the fabrication parameters to design membranes for tissue cultures and regeneration.
Department of Civil, Environmental, Aerospace, Materials Engineering (DICAM), University of Palermo, Viale delle Scienze Ed. 8, 90128 Palermo, Italy. Electronic address: salvatore.montesanto1985@gmail.com.
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Department of Civil, Environmental, Aerospace, Materials Engineering (DICAM), University of Palermo, Viale delle Scienze Ed. 8, 90128 Palermo, Italy.
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Brucato
Department of Civil, Environmental, Aerospace, Materials Engineering (DICAM), University of Palermo, Viale delle Scienze Ed. 8, 90128 Palermo, Italy; Euro-Mediterranean Institute of Science and Technology (IEMEST), Via Michele Miraglia, 20, 90128 Palermo, Italy.
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La Carrubba
D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D055119:Elastic Modulus; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D055595:Mechanical Phenomena; D008567:Membranes, Artificial; D010477:Perfusion; D011091:Polyesters / Q000494:pharmacology*; D016062:Porosity; D013314:Stress, Mechanical; D013696:Temperature; D013718:Tensile Strength
D008567:Membranes, Artificial; D011091:Polyesters; C033616:poly(lactide)
Cell adhesion; Mechanical properties; PLLA membranes