Effects of alprostadil and iloprost on renal, lung, and skeletal muscle injury following hindlimb ischemia-reperfusion injury in rats.
OBJECTIVE
To evaluate the effects of alprostadil (prostaglandin [PGE1] analog) and iloprost (prostacyclin [PGI2] analog) on renal, lung, and skeletal muscle tissues after ischemia reperfusion (I/R) injury in an experimental rat model.


METHODS
Wistar albino rats underwent 2 hours of ischemia via infrarenal aorta clamping with subsequent 2 hours of reperfusion. Alprostadil and iloprost were given starting simultaneously with the reperfusion period. Effects of agents on renal, lung, and skeletal muscle (gastrocnemius) tissue specimens were examined.


RESULTS
Renal medullary congestion, cytoplasmic swelling, and mean tubular dilatation scores were significantly lower in the alprostadil-treated group than those found in the I/R-only group (P<0.0001, P=0.015, and P<0.01, respectively). Polymorphonuclear leukocyte infiltration, pulmonary partial destruction, consolidation, alveolar edema, and hemorrhage scores were significantly lower in alprostadil- and iloprost-treated groups (P=0.017 and P=0.001; P<0.01 and P<0.0001). Polymorphonuclear leukocyte infiltration scores in skeletal muscle tissue were significantly lower in the iloprost-treated group than the scores found in the nontreated I/R group (P<0.0001).


CONCLUSIONS
Alprostadil and iloprost significantly reduce lung tissue I/R injury. Alprostadil has more prominent protective effects against renal I/R injury, while iloprost is superior in terms of protecting the skeletal muscle tissue against I/R injury.
Department of Cardiovascular Surgery.
Dilek
D
Erer
Department of Cardiovascular Surgery.
Abdullah
A
Özer
Department of Cardiovascular Surgery.
Hüseyin
H
Demirtaş
Department of Pathology, Gazi University Medical Faculty.
İpek Işık
İI
Gönül
Department of Pharmacology, Yıldırım Beyazıt University Medical Faculty.
Halil
H
Kara
Department of Oral and Maxillofacial Surgery, Ankara University Faculty of Dentistry, Besevler, Ankara.
Hande
H
Arpacı
Department of Physiology, Kırıkkale University Medical Faculty, Kırıkkale.
Faruk Metin
FM
Çomu
Department of Cardiovascular Surgery.
Gürsel Levent
GL
Oktar
Department of Anesthesiology and Reanimation, Gazi University Medical Faculty, Ankara.
Mustafa
M
Arslan
Department of Physiology, Dumlupınar University Medical Faculty, Kütahya, Turkey.
Ayşegül
A
Küçük
D000527:Alprostadil / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D004195:Disease Models, Animal; D006614:Hindlimb / Q000187:drug effects / Q000473:pathology; D016285:Iloprost / Q000737:chemistry / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology*
D000527:Alprostadil; D016285:Iloprost
alprostadil; iloprost; ischemia reperfusion injury; lung; rat; renal; skeletal muscle