Single-armed phenylsulfonated pyridine derivative of DOTA is rigid and stable paramagnetic tag in protein analysis.
Single-armed DOTA-like phenylsulfonated pyridine derivatives are rigid and stable paramagnetic tags for site-specific labeling of proteins. Their reactions with a solvent-exposed protein thiol group generate a stable C-S bond and produce one single paramagnetic species in solution NMR. The generated large paramagnetic effects yield valuable long-range structural restraints for proteins.
State Key Laboratory of Elemento-Organic Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China. xunchengsu@nankai.edu.cn.
Feng
F
Yang
Xiao
X
Wang
Bin-Bin
BB
Pan
Xun-Cheng
XC
Su
D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011506:Proteins / Q000032:analysis*; D011725:Pyridines / Q000737:chemistry*
D006573:Heterocyclic Compounds, 1-Ring; D011506:Proteins; D011725:Pyridines; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid