A Quiescent, Regeneration-Responsive Tissue Engineered Mesenchymal Stem Cell Bone Marrow Niche Model via Magnetic Levitation.
The bone marrow niche represents a specialized environment that regulates mesenchymal stem cell quiescence and self-renewal, yet fosters stem cell migration and differentiation upon demand. An in vitro model that embodies these features would open up the ability to perform detailed study of stem cell behavior. In this paper we present a simple bone marrow-like niche model, which comprises of nanomagnetically levitated stem cells cultured as multicellular spheroids within a type I collagen gel. The stem cells maintained are nestin positive and remain quiescent until regenerative demand is placed upon them. In response to coculture wounding, they migrate and appropriately differentiate upon engraftment. This tissue engineered regeneration-responsive bone marrow-like niche model will allow for greater understanding of stem cell response to injury and also facilitate as a testing platform for drug candidates in a multiwell plate format.
Emily Elizabeth Louise
EE
Lewis
Paul O'Gorman Leukaemia Research Centre, University of Glasgow , Shelley Road, Glasgow G11 0YN, United Kingdom.
Helen
H
Wheadon
Natasha
N
Lewis
Jingli
J
Yang
Margaret
M
Mullin
School of Science, University of the West of Scotland , Paisley PA1 2BE, United Kingdom.
Andrew
A
Hursthouse
School of Science, University of the West of Scotland , Paisley PA1 2BE, United Kingdom.
David
D
Stirling
Matthew John
MJ
Dalby
Catherine Cecilia
CC
Berry
D001853:Bone Marrow; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells; D012038:Regeneration; D055153:Stem Cell Niche; D023822:Tissue Engineering
bone marrow niche; differentiation; magnetic nanoparticles; mesenchymal stem cells; migration; scratch assay; tissue engineering; wound homing