Proteomic Analysis of Parkin Isoforms Expression in Different Rat Brain Areas.
PARK2 gene's mutations are related to the familial form of juvenile Parkinsonism, also known as the autosomic recessive juvenile Parkinsonism. This gene encodes for parkin, a 465-amino acid protein. To date, a large number of parkin isoforms, generated by an alternative splicing mechanism, have been described. Currently, Gene Bank lists 27 rat PARK2 transcripts, which matches to 20 exclusive parkin alternative splice variants. Despite the existence of these isoforms, most of the studies carried out so far, have been focused only on the originally cloned parkin. In this work we have analyzed the expression profile of parkin isoforms in some rat brain areas including prefrontal cortex, hippocampus, substantia nigra and cerebellum. To discriminate among these isoforms, we detected their localization through the use of two antibodies that are able to identify different domains of the parkin canonical sequence. Our analysis has revealed that at least fourteen parkin isoforms are expressed in rat brain with a various distribution in the regions analyzed. Our study might help to elucidate the pathophysiological role of these proteins in the central nervous system.
San Raffaele Open University of Rome, Rome, Italy.|Section of Human Anatomy and Histology, Department of Biomedical and Biotechnological Sciences, University of Catania, Via S.Sofia, 87, 95123, Catania, Italy.
Agata Grazia
AG
D'Amico
Section of Human Anatomy and Histology, Department of Biomedical and Biotechnological Sciences, University of Catania, Via S.Sofia, 87, 95123, Catania, Italy.
Grazia
G
Maugeri
Section of Human Anatomy and Histology, Department of Biomedical and Biotechnological Sciences, University of Catania, Via S.Sofia, 87, 95123, Catania, Italy.
Rita
R
Reitano
Institute of Neurological Sciences, Italian National Research Council, Catania, Italy.
Sebastiano
S
Cavallaro
Section of Human Anatomy and Histology, Department of Biomedical and Biotechnological Sciences, University of Catania, Via S.Sofia, 87, 95123, Catania, Italy. vdagata@unict.it.
Velia
V
D'Agata
D017398:Alternative Splicing; D000818:Animals; D002531:Cerebellum / Q000737:chemistry / Q000378:metabolism*; D015870:Gene Expression; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism*; D007525:Isoelectric Focusing; D008297:Male; D009928:Organ Specificity; D017397:Prefrontal Cortex / Q000737:chemistry / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000737:chemistry / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D020033:Protein Isoforms; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein
Parkin distribution; Parkin isoforms; Rat brain; Two-dimensional gel electrophoresis