Expression of IL-6, IL-10, IL-17 and IL-33 in the peri-implant crevicular fluid of patients with peri-implant mucositis and peri-implantitis.
OBJECTIVE
The aim of this study was to compare the levels of IL-6, IL-10, IL-17 and IL-33 in the peri-implantar crevicular fluid (PICF) and in parotid gland saliva (PGS) of healthy patients, and peri-implantitis and peri-implant mucositis patients.


METHODS
The PICF was collected from 40 implants as follows: 10 peri-implant mucositis patients, 20 peri-implantitis patients and 10 healthy patients. The PICF and PGS samples collected from each patient were quantified for IL-6, IL-10, IL-17 and IL-33 by enzymatic immunosorbent assay (ELISA).


RESULTS
IL-6, IL-17 and IL-33 levels on PIFC were significantly higher in peri-implantitis group when compared to healthy group. IL-17 and IL-33 levels in PIFC were significantly higher in peri-implant mucositis group than in healthy group. There was no significant difference when comparing IL-6, IL-10, IL-17 and IL-33 levels in PGS among healthy, peri-implant mucositis and peri-implantitis groups.


CONCLUSIONS
Therefore, as in patients with peri-implantitis there were significantly higher levels of IL-6, IL-17 and IL-33 in PICF, we believe that these cytokines were intensifying local inflammatory process, and contributing to clinical aspects such as increased marginal bleeding and probing depth found in patients with peri-implantitis. Furthermore, as IL-17 and IL-33 were increased in patients with peri-implant mucositis, hypothesized that these cytokines were also contributing to the inflammatory process observed in this disease.
Federal University of Triangulo Mineiro (UFTM), Brazil.
Viviane Oliveira
VO
Severino
Federal University of Triangulo Mineiro (UFTM), Brazil.
Marcela
M
Beghini
Federal University of Triangulo Mineiro (UFTM), Brazil.
Márcia Fernandes
MF
de Araújo
University of Uberaba, Brazil.
Marcelo Luiz Ribeiro
MLR
de Melo
Federal University of Triangulo Mineiro (UFTM), Brazil.
Camila Botelho
CB
Miguel
Federal University of Triangulo Mineiro (UFTM), Brazil.
Wellington Francisco
WF
Rodrigues
Federal University of Triangulo Mineiro (UFTM), Brazil; University of Uberaba, Brazil; Cefores, Federal University of Triangulo Mineiro (UFTM), Brazil. Electronic address: sanivia.pereira@uniube.br.
Sanivia Aparecida
SA
de Lima Pereira
D000328:Adult; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D000067596:Interleukin-33 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D052016:Mucositis / Q000378:metabolism*; D057873:Peri-Implantitis / Q000378:metabolism*
D016207:Cytokines; D020381:Interleukin-17; D000067596:Interleukin-33; D015850:Interleukin-6; D016753:Interleukin-10
Cytokines; Mucositis; Peri-implantitis