Isolation and Characterization of the 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) Radical Cation-Scavenging Reaction Products of Arbutin.
Arbutin, a glucoside of hydroquinone, has shown strong 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation-scavenging activity, especially in reaction stoichiometry. This study investigated the reaction mechanism of arbutin against ABTS radical cation that caused high stoichiometry of arbutin in an ABTS radical cation-scavenging assay. HPLC analysis of the reaction mixture of arbutin and ABTS radical cation indicated the existence of two reaction products. The two reaction products were purified and identified to be a covalent adduct of arbutin with an ABTS degradation fragment and 3-ethyl-6-sulfonate benzothiazolone. A time-course study of the radical-scavenging reactions of arbutin and the two reaction products suggested that one molecule of arbutin scavenges three ABTS radical cation molecules to generate an arbutin-ABTS fragment adduct as a final reaction product. The results suggest that one molecule of arbutin reduced two ABTS radical cation molecules to ABTS and then cleaved the third ABTS radical cation molecule to generate two products, an arbutin-ABTS fragment adduct and 3-ethyl-6-sulfonate benzothiazolone.
Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima , Shobara, Hiroshima 727-0023, Japan.
Akihiro
A
Tai
Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima , Shobara, Hiroshima 727-0023, Japan.
Asako
A
Ohno
Faculty of Health and Welfare Science, Okayama Prefectural University , Soja, Okayama 719-1197, Japan.
Hideyuki
H
Ito
D000975:Antioxidants / Q000737:chemistry*; D001104:Arbutin / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry*; D002412:Cations / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006873:Hydroquinones / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry*
D000975:Antioxidants; D052160:Benzothiazoles; D002412:Cations; D006873:Hydroquinones; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D001104:Arbutin; C031927:hydroquinone
ABTS radical cation; adduct formation; antioxidant; arbutin; radical-scavenging activity