Estrogen receptor α and β are involved in the activation of lordosis behavior in estradiol-primed rats.
The present study was designed to assess the participation of estrogen receptors alpha (ERα) and beta (ERβ) in the short-term facilitation of lordosis behavior in ovariectomized (ovx), estradiol (E2) primed rats. In experiment 1, dose response curves for PPT and DPN (ERα and ERβ agonists, respectively) facilitation of lordosis behavior (lordosis quotient and lordosis score) were established by infusing these agonists into the right lateral ventricle (icv) in female rats injected 40h previously with 5μg of E2benzoate. PPT doses of 0.08 and 0.4ng produced high lordosis quotients starting at 30min and continuing at 120 and 240min post-injection. DPN induced high levels of lordosis behavior at all times tested. However, the intensity of lordosis induced by both agonists was weak. In experiment 2, we tested the involvement of each ER in facilitation of lordosis by icv infusion of MPP (ERα-selective antagonist) or PHTPP (ERβ-selective antagonist) prior to infusion of 2ng of free E2. Icv infusion of either MPP or PHTPP 30min before free E2 significantly depressed E2facilitation of lordosis. The results suggest that both forms of ER are involved in the short-latency facilitation of lordosis behavior in E2-primed rats.
Centro de Investigación en Reproducción Animal, Universidad Autónoma de Tlaxcala-CINVESTAV, México; Doctorado en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, México.
Raymundo
R
Domínguez-Ordóñez
Centro de Investigación en Reproducción Animal, Universidad Autónoma de Tlaxcala-CINVESTAV, México.
Marcos
M
García-Juárez
Centro de Investigación en Reproducción Animal, Universidad Autónoma de Tlaxcala-CINVESTAV, México; Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tlaxcala, México.
Francisco J
FJ
Lima-Hernández
Centro de Investigación en Reproducción Animal, Universidad Autónoma de Tlaxcala-CINVESTAV, México.
Porfirio
P
Gómora-Arrati
Department of Psychological and Brain Sciences, University of Massachusetts, Amherst, MA 01003, USA.
Jeffrey D
JD
Blaustein
Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Anne M
AM
Etgen
Centro de Investigación en Reproducción Animal, Universidad Autónoma de Tlaxcala-CINVESTAV, México. Electronic address: oglezflo@gmail.com.
Oscar
O
González-Flores
D000818:Animals; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000502:physiology*; D047629:Estrogen Receptor beta / Q000819:agonists / Q000502:physiology*; D030762:Estrous Cycle / Q000187:drug effects / Q000502:physiology; D005260:Female; D008297:Male; D010052:Ovariectomy; D011187:Posture / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012726:Sexual Behavior, Animal / Q000187:drug effects / Q000502:physiology*
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004958:Estradiol
Estradiol; Estrogen receptor isoforms; Lordosis; Progesterone receptor