Screening for Neuraminidase Inhibitory Activity in Traditional Chinese Medicines Used to Treat Influenza.
To screen for influenza virus neuraminidase inhibition and to provide a reference for the clinical treatment of influenza using traditional Chinese medicines (TCM). In this study, 421 crude extracts (solubilized with petroleum ether, ethanol, ethyl acetate, and aqueous solvents) were obtained from 113 TCM. The medicine extracts were then reacted with oseltamivir, using 2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (MUNANA) as the substrate, to determine influenza virus neuraminidase activity using a standard fluorimetric assay. It was found that Chinese medicine extracts from Pyrola calliantha, Cynanchum wilfordii, Balanophora involucrata and Paeonia delavayi significantly inhibited neuraminidase activity at a concentration of 40 μg/mL. Dose-dependent inhibitory assays also revealed significant inhibition. The IC50 range of the TCM extracts for influenza virus neuraminidase was approximately 12.66-34.85 μg/mL, respectively. Some Chinese medicines have clear anti-influenza viral effects that may play an important role in the treatment of influenza through the inhibition of viral neuraminidase. The results of this study demonstrated that plant medicines can serve as a useful source of neuraminidase (NA) inhibitors and further investigation into the pharmacologic activities of these extracts is warranted.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. 18787292050@139.com.|College of Pharmacy and Chemistry, Dali University, Yunnan 671000, China. 18787292050@139.com.
Xian-Ying
XY
Yang
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. liuailin@imm.ac.cn.
Ai-Lin
AL
Liu
Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. shujing@mail.med.upenn.edu.
Shu-Jing
SJ
Liu
Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. xug@mail.med.upenn.edu.
Xiao-Wei
XW
Xu
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. lfhuang@implad.ac.cn.
Lin-Fang
LF
Huang
D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000627:therapeutic use; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000627:therapeutic use; D006801:Humans; D007251:Influenza, Human / Q000188:drug therapy; D008516:Medicine, Chinese Traditional; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D014764:Viral Proteins / Q000037:antagonists & inhibitors*
D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase
neuraminidase inhibition; screening; traditional Chinese medicine