Substance P incorporation in calcium phosphate cement for dental alveolar bone defect restoration.
A combination of osteoinductive neuropeptide substance P (SP) and osteoconductive bone cement of calcium phosphate (CPC) might provide an effective and lower-cost solution for complex alveolar bone defects restoration. The present study aims to investigate the key design considerations of SP delivery in CPC. In this study, CPC-based modular scaffolds were developed, where collagen type I was used as accessory organic ingredient to modulate the physical and biological characters. SP was directly mixed in the cement as free peptides, or was covalently immobilized with collagen component. The structural and mechanical properties of the scaffolds were assessed in vitro, and their osteogenic ability was observed in a rabbit model with alveolar bone defect. The results showed that SP could enhance the osteo-conductivity/inductivity of CPC. Collagen solution optimized biocompatibility of CPC, and meanwhile exhibited additive effects on the functions of SP. Nevertheless, immobilization of SP with collagen blocked their bioactivity in CPC. Collagen sponges created macro-porosity in CPC and achieved maximum bone ingrowth with the aid of SP. In conclusion, the present study primarily demonstrated that CPC scaffold can be functionalized by synthetic SP, and the biocompatibility and porosity of the scaffold are adaptable key factors determining their final osteogenic activities.
Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
Tianjue
T
Wang
Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
Di
D
Wu
Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
Yuan
Y
Li
Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
Wantao
W
Li
Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
Shuyin
S
Zhang
Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
Kaijin
K
Hu
Department of Oral and Maxillofacial Surgery, School of Stomatology, State Key Laboratory of Military Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China. Electronic address: hzzhou@fmmu.edu.cn.
Hongzhi
H
Zhou
D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000601:surgery / Q000628:therapy*; D000818:Animals; D001843:Bone Cements / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects; D002130:Calcium Phosphates / Q000494:pharmacology*; D003793:Dental Restoration, Permanent; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology / Q000601:surgery; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D051381:Rats; D013373:Substance P / Q000494:pharmacology*; D054457:Tissue Scaffolds / Q000737:chemistry; D014961:X-Ray Diffraction; D055114:X-Ray Microtomography
D001843:Bone Cements; D002130:Calcium Phosphates; D013373:Substance P; C020243:calcium phosphate
Bone regeneration; Calcium phosphate cement; Collagen; Scaffold; Substance P