Evidence of vertical transmission of ostreid herpesvirus 1 in the Portuguese oyster Crassostrea angulata.
In order to determine if ostreid herpesvirus 1 (OsHV-1) can be vertically transmitted, 9 full-sib families of the Portuguese oyster Crassostrea angulata were produced using a factorial mating design with 3 males and 3 females. The parents were survivors from an OsHV-1 mortality outbreak. OsHV-1 DNA was not detected by conventional PCR in the mantle of parents, gametes or 3day-old larvae. However, viral DNA was detected by real-time PCR in all gametes and larvae samples. These results show that C. angulata that have survived an OsHV-1 mortality outbreak can carry the virus and vertically transmit it to their offspring.
Instituto de Investigación y Formación Agraria y Pesquera (IFAPA), Centro Agua del Pino, Junta de Andalucía, Ctra. El Rompido-Punta Umbría, km 4, 21459 Cartaya, Spain. Electronic address: monserratls@yahoo.es.
Monserrat
M
López Sanmartín
Centro de Ciências do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
Deborah M
DM
Power
Departamento de Genética de la Universidad de Granada, Spain.
Roberto
R
de la Herrán
Instituto de Investigación y Formación Agraria y Pesquera (IFAPA), Centro Agua del Pino, Junta de Andalucía, Ctra. El Rompido-Punta Umbría, km 4, 21459 Cartaya, Spain.
José I
JI
Navas
Centro de Ciências do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal; Instituto Português do Mar e da Atmosfera, Divisão de Aquicultura e Valorização, Estação Experimental de Moluscicultura de Tavira, Av. 5 de Outubro, 8700-305 Olhão, Portugal.
Frederico M
FM
Batista
D000818:Animals; D049896:Crassostrea / Q000821:virology*; D005260:Female; D006564:Herpesviridae; D006566:Herpesviridae Infections / Q000662:veterinary*; D018445:Infectious Disease Transmission, Vertical; D007814:Larva / Q000821:virology; D008297:Male; D060888:Real-Time Polymerase Chain Reaction
Crassostrea angulata; Offspring; OsHV-1; Swim up; Vertical transmission