In vitro production of Toxocara canis excretory-secretory (TES) antigen.
Toxocara canis is a widespread gastrointestinal nematode parasite of dogs and cause Toxocara larva migrans, an important zoonotic disease in humans on ingestion of infective eggs. Toxocarosis is one of the few human parasitic diseases whose serodiagnosis uses a standardized antigen, T. canis excretory secretory antigen (TES). The present study describes collection of T. canis adult worm, collection and embryonation of T. canis eggs, hatching and separation of T. canis larvae, in vitro maintenance of T. canis second stage larvae for production of TES, concentration of culture fluid TES and yield of TES in correlation with various methods cited in literature.
Department of Veterinary Parasitology, Madras Veterinary College (TANUVAS), Chennai, India.
Divyamol
D
Thomas
Department of Veterinary Parasitology, Veterinary College and Research Institute (TANUVAS), Orathanadu, India.
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N
Jeyathilakan
Department of Veterinary Parasitology, Madras Veterinary College (TANUVAS), Chennai, India.
S
S
Abdul Basith
Department of Animal Biotechnology, Madras Veterinary College (TANUVAS), Chennai, India.
T M A
TM
Senthilkumar
Excretory-secretory antigen; In vitro cultivation; Toxocara canis