Identification of an H2O2permeable PIP aquaporin in barley and a serine residue promoting H2O2transport.
A barley (Hordeum vulgare) plasma membrane type aquaporin, HvPIP2;5, was identified as an H2O2permeable aquaporin among 21 barley and rice PIPs examined in the heterologous expression system using Saccharomyces cerevisiae. Four TIPs were also detected as H2O2-transporting aquaporins among 15 barley and rice TIPs. Influx of H2O2into yeast cells expressing HvPIP2;5 was determined with a florescent-dye-based assay. Indirect immunofluorescence indicated that the expression of HvPIP2;5 protein was ubiquitous in root tissues, and was also weakly observed in leaf epidermal cells and cells in the vascular bundle. Point mutated variants of HvPIP2;5 were generated by the site-directed mutagenesis. Growth assays of yeast cells expressing these mutated HvPIP2;5 proteins suggested that Ser-126 in HvPIP2;5 has a large impact on H2O2transport with a minor influence on the HvPIP2;5-mediated water transport.
Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046, Japan.|Faculty of Sciences, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic.
Jiye
http://orcid.org/0000-0002-1943-869X
J
Rhee
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, 386-8567, Japan.
Tomoaki
T
Horie
Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046, Japan.
Shizuka
S
Sasano
Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046, Japan.|Research Fellow of the Japan Society for the Promotion of Science, 5-3-1 Kojimachi, Chiyoda-ku, Tokyo, 102-0083, Japan.
Yoshiki
Y
Nakahara
Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046, Japan.
Maki
M
Katsuhara
D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D001467:Hordeum / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009928:Organ Specificity; D012275:Oryza / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D019441:Plant Epidermis / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D012694:Serine / Q000378:metabolism
D020346:Aquaporins; D010940:Plant Proteins; D012694:Serine; D006861:Hydrogen Peroxide