In vitro cytogenetic evaluation of the particular combination of flurbiprofen and roxithromycin.
Flurbiprofen (FLB) (anti-inflammatory and analgesic drug) and roxithromycin (RXM) (antibiotic) were widely used in world wide. This study deals with investigation of genotoxicity, cytotoxicity, and oxidative stress effects of a particular combination of these drugs in human cultured lymphocytes. Also, DNA damaging-protective effects of combination of these drugs were analyzed on plasmid DNA. Human lymphocytes were treated with different concentrations (FLB + RXM; 10 μg/mL + 25 μg/mL, 15 μg/mL + 50 μg/mL, and 20 μg/mL + 100 μg/mL) of the drugs following by study of their genotoxic and cytotoxic effects by analysis of cytokinesis-block micronucleus test and nuclear division index, respectively. The effect of the combination in aspect of anti-oxidative and DNA damaging activity was evaluated on Pet-22b plasmid. According to our results, the combination of FLB and RXM did not show a notable genotoxic effect on cells. Although each of the substances had been shown as a cytotoxic agent by previous researchers, in this research, the combination of these drugs did not exhibit any adverse effect on cell division. FLB had DNA protection effect against H2O2while in combination with RXM had not the same effect on the plasmid.
a Department of Biology , Institute of Science, Cukurova University , Adana , Turkey.
Taygun
T
Timocin
a Department of Biology , Institute of Science, Cukurova University , Adana , Turkey.
Mehmet Tahir
MT
Husunet
b Department of Molecular Biology and Genetics , Faculty of Sciences and Arts, Bülent Ecevit University , Turkey.
Ebrahim
E
Valipour
c Department of Biotechnology , Institute of Science, Cukurova University , Adana , Turkey , and.
Mostafa
M
Norizadeh Tazehkand
a Department of Biology , Institute of Science, Cukurova University , Adana , Turkey.
Rima
R
Celik
d Department of Biology , Faculty of Science and Letters, Cukurova University , Adana , Turkey.
Mehmet
M
Topaktas
d Department of Biology , Faculty of Science and Letters, Cukurova University , Adana , Turkey.
Hasan B
HB
Ila
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005260:Female; D005480:Flurbiprofen / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D018384:Oxidative Stress / Q000187:drug effects; D010957:Plasmids; D015575:Roxithromycin / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D055815:Young Adult
D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D015575:Roxithromycin; D005480:Flurbiprofen
Flurbiprofen; Roxithromycin; cytotoxicity; genotoxicity; micronucleus; oxidative stress