[Letter to the Editor] NaOH concentration and streptavidin bead type are key factors for optimal DNA aptamer strand separation and isolation.
Address correspondence to Geoffrey K. Kilili or Christine M. Karbiwnyk, Winchester Engineering and Analytical Center, US Food and Drug Administration, Winchester, MA, 01890. E-mail: Geoffrey.Kilili@fda.hhs.gov or Christine.Karbiwnyk@fda.hhs.gov.
Winchester Engineering and Analytical Center, US Food and Drug Administration, Winchester, MA.
Geoffrey K
GK
Kilili
Winchester Engineering and Analytical Center, US Food and Drug Administration, Winchester, MA.
Lianna
L
Tilton
Winchester Engineering and Analytical Center, US Food and Drug Administration, Winchester, MA.
Christine M
CM
Karbiwnyk
D052157:Aptamers, Nucleotide / Q000302:isolation & purification*; D004247:DNA / Q000302:isolation & purification*; D008863:Microspheres; D052156:SELEX Aptamer Technique / Q000379:methods*; D012972:Sodium Hydroxide / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D012972:Sodium Hydroxide; D004247:DNA; D019809:Streptavidin
SELEX; single-stranded DNA (ssDNA); sodium hydroxide; streptavidin magnetic beads