Turn-On Colorimetric Platform for Dual Activity Detection of Acid and Alkaline Phosphatase in Human Whole Blood.
The activity detection of acid phosphatase (ACP) and alkaline phosphatase (ALP) is of great importance to the diagnosis and prognosis of related diseases. In this work, we report for the first time a turn-on colorimetric platform for the activity detection of ACP and ALP, by exploiting Cu(BCDS)22-(BCDS=bathocuproinedisulfonate) as the probe. The presence of ACP or ALP dephosphorylates the substrate ascorbic acid 2-phosphate to produce ascorbic acid, which then reduces Cu(BCDS)22-into Cu(BCDS)23-, leading to a turn-on spectral absorption at 484 nm and a dramatic color change of the solution from colorless to orange-red. The underlying metal-to-ligand charge-transfer mechanism has been demonstrated by quantum mechanical computations. This platform allows a rapid, sensitive readout of ACP and ALP activities within the dynamic range from 0 to 220 mU ml-1. In addition, it is highly immune to false-positive results and also highly selective. More importantly, it is applicable in the presence of human serum and even whole blood samples. These results demonstrate that our platform holds great potential in clinical practices and in the point-of-care analysis.
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China.
Qiong
Q
Hu
School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China.
Baojing
B
Zhou
American Advanced Nanotechnology, Houston, TX, 77459, USA.
Feng
F
Li
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China.
Jinming
http://orcid.org/0000-0001-5721-6513
J
Kong
Chemistry Department, College of Arts and Sciences, University of South Florida, Tampa, FL, 33620-4202, USA.
Xueji
X
Zhang
D000135:Acid Phosphatase / Q000097:blood*; D000469:Alkaline Phosphatase / Q000097:blood*; D003124:Colorimetry / Q000379:methods*; D056831:Coordination Complexes / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008968:Molecular Conformation; D010084:Oxidation-Reduction; D013053:Spectrophotometry
D056831:Coordination Complexes; D003300:Copper; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase
colorimetric; density functional calculations; metal-to-ligand charge-transfer; phosphatase; transition metals