Protective Effects of Intralipid and Caffeic Acid Phenyl Esther (CAPE) on Neurotoxicity Induced by Ethanol in Rats.
OBJECTIVE
Ethanol causes oxidative degradation of the mitochondrial genome in the brain. This effect could contribute to the development of brain injury in some alcoholic patients. We investigated the protective effect of caffeic acid phenyl esther (CAPE) and intralipid (IL) on oxidative stress and neurotoxicity induced by ethanol intake.


METHODS
The forty-eight rats were randomly divided into seven groups. Ethanol was administered for acute toxicity. IL and CAPE were administered immediately after ethanol intake. Total oxidant status (TOS), total antioxidant status (TAS), and oxidative status index (OSi) were evaluated and histologic examination of cerebellum and brain tissue with Hematoxylin-Eosin and immuno-histochemical dyes was performed.


RESULTS
In the ethanol group, TAS levels were significantly lower than the other groups and this finding indicates that the toxic effect of ethanol reduces antioxidant levels. In the ethanol group, TOS levels were significantly higher than the other groups. These results showed that ethanol induced oxidative stress. IL treatment increased TAS levels, and CAPE decreased TOS levels against ethanol toxicity. There was correlation between TAS and TOS levels. Also, histopathologic results confirmed these biochemical results.


CONCLUSIONS
CAPE and IL treatment could be effective course of therapy to enhance therapeutic efficacy and may provide a promising approach for the treatment of neurotoxicity and oxidative stress induced by ethanol in clinic.
Mustafa Kemal University, Medical Faculty, Department of Neurosurgery, Hatay, Turkey.
Seyit Kagan
SK
Basarslan
Arif
A
Osun
Serkan
S
Senol
Murat
M
Korkmaz
Umit
U
Ozkan
Ibrahim
I
Kaplan
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002109:Caffeic Acids / Q000494:pharmacology*; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004655:Emulsions / Q000494:pharmacology; D000431:Ethanol / Q000633:toxicity*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology*; D051381:Rats; D013024:Soybean Oil / Q000494:pharmacology*
D000975:Antioxidants; D002109:Caffeic Acids; D004655:Emulsions; D018696:Neuroprotective Agents; D010743:Phospholipids; C545823:soybean oil, phospholipid emulsion; D000431:Ethanol; D013024:Soybean Oil; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol