Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2: Detection of Breast Cancer in a Live Tissue.
We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10-50cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90-130 μm in live mouse tissue by TPM.
KU-KIST Graduate School of Converging Science and Technology, Korea University , 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
Ji-Woo
JW
Choi
KU-KIST Graduate School of Converging Science and Technology, Korea University , 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
Seung Taek
ST
Hong
Department of Chemistry, Korea University , 145 Anam-ro, Sungbuk-gu, Seoul, 02841, Republic of Korea.
Dong Eun
DE
Kang
Department of Chemistry, Daejin University , 1007 Hoguk-ro, Pochun-si, Gyeonggi-do 11159, Republic of Korea.
Kyu Cheol
KC
Paik
Department of Chemistry, Daejin University , 1007 Hoguk-ro, Pochun-si, Gyeonggi-do 11159, Republic of Korea.
Man So
MS
Han
Department of Chemistry, Ajou University , 206 Worldcup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do 16499, Republic of Korea.
Chang Su
CS
Lim
KU-KIST Graduate School of Converging Science and Technology, Korea University , 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.|Department of Chemistry, Korea University , 145 Anam-ro, Sungbuk-gu, Seoul, 02841, Republic of Korea.|Department of Chemistry, Daejin University , 1007 Hoguk-ro, Pochun-si, Gyeonggi-do 11159, Republic of Korea.
Bong Rae
BR
Cho
D000818:Animals; D001572:Benzofurans / Q000138:chemical synthesis / Q000494:pharmacology* / Q000528:radiation effects; D001943:Breast Neoplasms / Q000000981:diagnostic imaging*; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000494:pharmacology* / Q000528:radiation effects; D006801:Humans; D008027:Light; D008807:Mice, Inbred BALB C; D011719:Pyrazines / Q000138:chemical synthesis / Q000494:pharmacology* / Q000528:radiation effects; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis / Q000494:pharmacology* / Q000528:radiation effects
D001572:Benzofurans; D005456:Fluorescent Dyes; D011719:Pyrazines; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins; C508053:ERBB2 protein, human