The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots.
Indirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and to assess their response to specific stimuli, we compared the biological, functional and ultrastructural properties of two ASC pools derived from mediastinal and subcutaneous depots (thoracic compartment) by means of supplements such as platelet lysate (PL) and FBS. Subcutaneous ASCs exhibited higher proliferative and clonogenic abilities than mediastinal counterpart, as well as increased secreted levels of IL-6 combined with lower amount of VEGF-C. In contrast, mediastinal ASCs displayed enhanced pro-angiogenic and adipogenic differentiation properties, increased cell diameter and early autophagic processes, highlighted by electron microscopy. Our results further support the hypothesis that the origin of adipose tissue significantly defines the biological properties of ASCs, and that a homogeneric function for all ASCs cannot be assumed.
Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: cami.siciliano@gmail.com.
Camilla
C
Siciliano
Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: anto.bordin@libero.it.
Antonella
A
Bordin
Division of Thoracic Surgery, Department of Medical-Surgical Science and Translational Medicine, Sapienza University of Rome, S. Andrea Hospital, via di Grottarossa 1035, 00189 Rome, Italy. Electronic address: mohsen.ibrahim@uniroma1.it.
Mohsen
M
Ibrahim
Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: isotta.chimenti@uniroma1.it.
Isotta
I
Chimenti
Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Section of Anatomy, Electron Microscopy Unit, Laboratory "Pietro M. Motta", Sapienza University of Rome, Rome, Italy. Electronic address: fra.cassiano@gmail.com.
Francesco
F
Cassiano
Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: ilenia.gatto@uniroma1.it.
Ilenia
I
Gatto
Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: gmangino@libero.it.
Giorgio
G
Mangino
Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: andrea.coccia@uniroma1.it.
Andrea
A
Coccia
Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Section of Anatomy, Electron Microscopy Unit, Laboratory "Pietro M. Motta", Sapienza University of Rome, Rome, Italy. Electronic address: selenia.miglietta@uniroma1.it.
Selenia
S
Miglietta
Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: daniela.bastianelli@uniroma1.it.
Daniela
D
Bastianelli
Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: vincenzo.petrozza@uniroma1.it.
Vincenzo
V
Petrozza
Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: antonella.calogero@uniroma1.it.
Antonella
A
Calogero
Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy; Department of AngioCardioNeurology, IRCCS NeuroMed, 86077 Pozzilli, IS, Italy. Electronic address: fraticello@inwind.it.
Giacomo
G
Frati
Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, Italy. Electronic address: elena.defalco@uniroma1.it.
Elena
E
De Falco
D050156:Adipogenesis; D000368:Aged; D057134:Antibodies, Neutralizing / Q000276:immunology; D001343:Autophagy; D002454:Cell Differentiation; D049109:Cell Proliferation; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008482:Mediastinum; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D050151:Subcutaneous Fat / Q000166:cytology*; D042582:Vascular Endothelial Growth Factor C / Q000276:immunology / Q000378:metabolism
D057134:Antibodies, Neutralizing; D015850:Interleukin-6; D042582:Vascular Endothelial Growth Factor C
Adipose tissue; Mesenchymal stem cells; Platelet lysate angiogenesis; Transmission electron microscopy