Rapid detection and identification of Stachybotrys and Chaetomium species using tissue PCR analysis.
Indoor fungi are a worldwide problem causing negative health effects for infected building's occupants and even deterioration of building structures. Different fungal species affect buildings and their inhabitants differently. Therefore, rapid and accurate identification of fungi to the species level is essential for health risk assessment and building remediation. This study focuses on molecular identification of two common indoor fungal genera: Stachybotrys and Chaetomium. This study proposes two new DNA barcode candidates for Stachybotrys and Chaetomium: the gene encoding mitogen activated protein kinase (hogA) and the intergenic region between histone 3 and histone 4 (h3-h4) as well as it introduces a rapid - 3.5h - protocol for direct Stachybotrys and Chaetomium species identification, which bypasses culture cultivation, DNA extraction and DNA sequencing.
Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, Denmark. Electronic address: anlew@bio.dtu.dk.
Anna M
AM
Lewińska
Danish Building Research Institute, Aalborg University, Copenhagen, SV, Denmark.
Ruut H
RH
Peuhkuri
Department of Civil Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark.
Carsten
C
Rode
Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, Denmark.
Birgitte
B
Andersen
Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, Denmark. Electronic address: jblni@dtu.dk.
Jakob B
JB
Hoof
D000391:Air Microbiology; D016902:Air Pollution, Indoor / Q000032:analysis*; D001483:Base Sequence; D002597:Chaetomium / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D058893:DNA Barcoding, Taxonomic / Q000379:methods; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D004784:Environmental Monitoring / Q000379:methods; D005800:Genes, Fungal; D006657:Histones / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D018570:Risk Assessment / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D013191:Stachybotrys / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D013997:Time Factors
D004271:DNA, Fungal; D006657:Histones; D020928:Mitogen-Activated Protein Kinases
DNA barcoding; ITS; Indoor fungi; PCR; Species identification