Chemical profiling of anti-hepatocellular carcinoma constituents from Caragana tangutica Maxim. by off-line semi-preparative HPLC-NMR.
An EtOAc fraction from the roots of Caragana tangutica Maxim. (CTEA) displayed promising anti-hepatocellular carcinoma (HCC) activity during screening of a traditional Chinese ethnic herb library against HepG2 and Hep3B cell lines. HPLC-based activity profiling of CTEA by combination of MS-guided large-scale semi-preparative HPLC and NMR methods led to the identification of a new pterocarpan glycoside, (-)-maackiain 3-O-6'-O-methyl malonyl-β-d-glucopyranoside (1), together with three known pterocarpan glycosides, (-)-maackiain 3-O-β-d-glucopyranoside (2), 3-O-6'-O-acrylyl-β-d-galactopyranoside (3), and (-)-maackiain 3-O-6'-O-acetyl-β-d-glucopyranoside (4). Compound 1 was isolated during a drug discovery programme aimed at identifying new anti-HCC leads from a natural product library. Anti-HCC study showed that all four compounds exhibited cytotoxic activity with IC50values range of 29.1-53.5 μg/mL against HepG2 and Hep3B cell lines.
a School of Pharmaceutical Sciences , South-Central University for Nationalities , Wuhan , China.
Xinzhou
X
Yang
a School of Pharmaceutical Sciences , South-Central University for Nationalities , Wuhan , China.
Mi
M
Huang
b School of Life Science and Bio-pharmaceutical , Guangdong Pharmaceutical University , Guangzhou , China.
Jinyan
J
Cai
c School of Medicine , Wuhan University of Science and Technology , Wuhan , China.
Dan
D
Lv
a School of Pharmaceutical Sciences , South-Central University for Nationalities , Wuhan , China.
Jingnan
J
Lv
a School of Pharmaceutical Sciences , South-Central University for Nationalities , Wuhan , China.
Sijian
S
Zheng
a School of Pharmaceutical Sciences , South-Central University for Nationalities , Wuhan , China.
Xinhua
X
Ma
a School of Pharmaceutical Sciences , South-Central University for Nationalities , Wuhan , China.
Ping
P
Zhao
c School of Medicine , Wuhan University of Science and Technology , Wuhan , China.
Qiang
Q
Wang
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D032602:Caragana / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004354:Drug Screening Assays, Antitumor; D005960:Glucosides / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018609:Tibet; D035721:Pterocarpus; D000094162:Phytoalexins
D000972:Antineoplastic Agents, Phytogenic; D005960:Glucosides; D010936:Plant Extracts; C000618487:maackiain 3-O-6'-O-methyl malonyl-beta-D-glucopyranoside; D000094162:Phytoalexins
Anti-hepatocellular carcinoma; Caragana tangutica; Tibetan medicines; pterocarpan glycosides