To Evaluate Cytotoxicity of Resin-based Restorative Materials on Human Lymphocytes by Trypan Blue Exclusion Test: An in vitro Study.
The cytotoxicity of a resin-based material can be evaluated on isolated human lymphocyte. Since resin-based dental materials have been used with increasing frequency in anterior and posterior teeth restorations, the uncured resin monomers are leached out from the restorations and diffuses into the dentine and ultimately hampers the odontoblastic layers of pulp as well as gingiva. It is also reaches into the saliva and circulatory blood. The study evaluates and compares the relative cytotoxicity of resin-based dental materials at different time interval, i.e. 24, 48, and 72 hours on human lymphocyte by Trypan blue exclusion method. All resins were found to be cytotoxic to human lymphocyte. Resin samples cytotoxicity was the highest in first 24 hours followed by 48 and 72 hours.
Professor and Head, Department of Pedodontics and Preventive Dentistry, Saraswati Dental College, Lucknow, Uttar Pradesh India.
Vinay Kumar
VK
Srivastava
MDS (PG-III), Department of Pedodontics and Preventive Dentistry, Saraswati Dental College, Lucknow, Uttar Pradesh, India.
Rajat Kumar
RK
Singh
Professor and Head, Department of General Pathology, Saraswati Dental College, Lucknow, Uttar Pradesh, India.
S N
SN
Malhotra
Reader, Department of Microbiology, Saraswati Dental College, Lucknow, Uttar Pradesh, India.
Aditi
A
Singh
Cytotoxicity; HISEP LSM; Nanocomposite; Trypan blue.