Variation of Human Salivary O-Glycome.
The study of saliva O-glycosylation is receiving increasing attention due to the potential of glycans for disease biomarkers, but also due to easy access and non-invasive collection of saliva as biological fluid. Saliva is rich in glycoproteins which are secreted from the bloodstream or produced by salivary glands. Mucins, which are highly O-glycosylated proteins, are particularly abundant in human saliva. Their glycosylation is associated with blood group and secretor status, and represents a reservoir of potential disease biomarkers. This study aims to analyse and compare O-glycans released from whole human mouth saliva collected 3 times a day from a healthy individual over a 5 days period. O-linked glycans were released by hydrazinolysis, labelled with procainamide and analysed by ultra-high performance liquid chromatography with fluorescence detection (UHPLC-FLR) coupled to electrospray ionization mass spectrometry (ESI-MS/MS). The sample preparation method showed excellent reproducibility and can therefore be used for biomarker discovery. Our data demonstrates that the O-glycosylation in human saliva changes significantly during the day. These changes may be related to changes in the salivary concentrations of specific proteins.
Ludger Ltd., Culham Science Centre, Oxfordshire, United Kingdom.
Radoslaw P
RP
Kozak
Ludger Ltd., Culham Science Centre, Oxfordshire, United Kingdom.
Paulina A
PA
Urbanowicz
School of Biomedical Sciences, Institute of Health and Biomedical Innovations, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, Australia.
Chamindie
C
Punyadeera
Centre for Proteomics and Metabolomics Leiden University Medical Centre, Leiden, The Netherlands.
Karli R
KR
Reiding
Centre for Proteomics and Metabolomics Leiden University Medical Centre, Leiden, The Netherlands.
Bas C
BC
Jansen
Ludger Ltd., Culham Science Centre, Oxfordshire, United Kingdom.
Louise
L
Royle
Ludger Ltd., Culham Science Centre, Oxfordshire, United Kingdom.
Daniel I
DI
Spencer
Ludger Ltd., Culham Science Centre, Oxfordshire, United Kingdom.
Daryl L
DL
Fernandes
Centre for Proteomics and Metabolomics Leiden University Medical Centre, Leiden, The Netherlands.
Manfred
M
Wuhrer
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D060748:Fetuins / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D008137:Longitudinal Studies; D013058:Mass Spectrometry; D055442:Metabolome; D011134:Polysaccharides / Q000378:metabolism*; D011342:Procainamide / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012463:Saliva / Q000378:metabolism*; D013194:Staining and Labeling
D060748:Fetuins; D011134:Polysaccharides; D011342:Procainamide