Inhibition of Staphylococcus aureus biofilm by a copper-bearing 317L-Cu stainless steel and its corrosion resistance.
The present study investigated the antibacterial performance, corrosion resistance and surface properties of antibacterial austenitic 317L-Cu stainless steel (317L-Cu SS). After 4.5wt% copper was added to 317L stainless steel (317L SS), the new alloy underwent solid solution and aging heat treatment. Fluorescent staining using 4',6-diamidino-2-phenylindole (DAPI) revealed that the 317L-Cu SS showed strong antibacterial efficacy, achieving a 99% inhibition rate of sessile Staphylococcus aureus cells after 5days. The corrosion data obtained by potentiodynamic polarization curves indicated that in comparison with 317L SS, the pitting potential and corrosion current density of 317L-Cu slightly decreased due to the addition of Cu. The 317L-Cu SS exhibited no cytotoxicity against zebrafish (Danio rerio) embryos. The experimental results in this study demonstrated that the new alloy has potential applications in medical and daily uses.
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400030, China.
Da
D
Sun
Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China. Electronic address: xudake@imr.ac.cn.
Dake
D
Xu
Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
Chunguang
C
Yang
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400030, China.
Jia
J
Chen
Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
M Babar
MB
Shahzad
Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
Ziqing
Z
Sun
Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
Jinlong
J
Zhao
Department of Chemical and Biomolecular Engineering, Institute for Corrosion and Multiphase Technology, Ohio University, Athens, OH 45701, USA.
Tingyue
T
Gu
Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
Ke
K
Yang
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400030, China. Electronic address: wanggx@cqu.edu.cn.
Guixue
G
Wang
D000818:Animals; D018441:Biofilms / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D003300:Copper / Q000494:pharmacology*; D003343:Corrosion; D004625:Embryo, Nonmammalian / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D056951:Photoelectron Spectroscopy; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013193:Stainless Steel / Q000494:pharmacology* / Q000633:toxicity; D013211:Staphylococcus aureus / Q000187:drug effects*; D013499:Surface Properties; D018675:Toxicity Tests; D015027:Zebrafish / Q000196:embryology
D011135:Polysaccharides, Bacterial; D013193:Stainless Steel; D003300:Copper
317L-Cu; Antibacterial; Biofilm; Cytotoxicity; Staphylococcus aureus