A Global eDNA Comparison of Freshwater Bacterioplankton Assemblages Focusing on Large-River Floodplain Lakes of Brazil.
With its network of lotic and lentic habitats that shift during changes in seasonal connection, the tropical and subtropical large-river systems represent possibly the most dynamic of all aquatic environments. Pelagic water samples were collected from Brazilian floodplain lakes (total n = 58) in four flood-pulsed systems (Amazon [n = 21], Araguaia [n = 14], Paraná [n = 15], and Pantanal [n = 8]) in 2011-2012 and sequenced via 454 for bacterial environmental DNA using 16S amplicons; additional abiotic field and laboratory measurements were collected for the assayed lakes. We report here a global comparison of the bacterioplankton makeup of freshwater systems, focusing on a comparison of Brazilian lakes with similar freshwater systems across the globe. The results indicate a surprising similarity at higher taxonomic levels of the bacterioplankton in Brazilian freshwater with global sites. However, substantial novel diversity at the family level was also observed for the Brazilian freshwater systems. Brazilian freshwater bacterioplankton richness was relatively average globally. Ordination results indicate that Brazilian bacterioplankton composition is unique from other areas of the globe. Using Brazil-only ordinations, floodplain system differentiation most strongly correlated with dissolved oxygen, pH, and phosphate. Our data on Brazilian freshwater systems in combination with analysis of a collection of freshwater environmental samples from across the globe offers the first regional picture of bacterioplankton diversity in these important freshwater systems.
Sackler Institute for Comparative Genomics, American Museum of Natural History, Central Park W. at 79th St., New York, NY, 10024, USA.|Richard Gilder Graduate School, American Museum of Natural History, Central Park W. at 79th St., New York, NY, 10024, USA.
Michael
M
Tessler
Sackler Institute for Comparative Genomics, American Museum of Natural History, Central Park W. at 79th St., New York, NY, 10024, USA.|Richard Gilder Graduate School, American Museum of Natural History, Central Park W. at 79th St., New York, NY, 10024, USA.|Biological Sciences Department, NYC College of Technology (CUNY), 300 Jay St., Brooklyn, NY, 11201, USA.
Mercer R
MR
Brugler
Sackler Institute for Comparative Genomics, American Museum of Natural History, Central Park W. at 79th St., New York, NY, 10024, USA.
Rob
R
DeSalle
Sackler Institute for Comparative Genomics, American Museum of Natural History, Central Park W. at 79th St., New York, NY, 10024, USA.
Rebecca
R
Hersch
Universidade Estadual de Maringá, Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura - Nupelia, Av. Colombo, 5790 - Bloco G-90, Maringá, PR, 87020-900, Brasil.
Luiz Felipe M
LFM
Velho
Universidade Estadual de Maringá, Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura - Nupelia, Av. Colombo, 5790 - Bloco G-90, Maringá, PR, 87020-900, Brasil.
Bianca T
BT
Segovia
Universidade Estadual de Maringá, Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura - Nupelia, Av. Colombo, 5790 - Bloco G-90, Maringá, PR, 87020-900, Brasil.
Fabio A
FA
Lansac-Toha
Biology Department, University of Illinois Springfield, One University Plaza, MS HSB223, Springfield, IL, 62703, USA. mlemk1@uis.edu.
Michael J
MJ
Lemke
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D044822:Biodiversity; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D017753:Ecosystem; D055868:Floods; D060106:Lakes / Q000382:microbiology*; D010933:Plankton / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
Araguaia; Bacterioplankton; Brazil; Diversity; Floodplain lakes; Metagenomics; Pantanal Amazon; Paraná