Chemical and structural characterization of Pholiota nameko extracts with biological properties.
Edible mushrooms including Pholiota nameko are excellent sources of extractable bioactive compounds with much to explore. Enzymatic extractions with Cellulase and Viscozyme were responsible for highest extraction yields (67-77%). No strong antioxidant activity was observed although extracts were able to scavenge ABTS(+) and OH(+). Potential prebiotic activity was observed in all extracts, some increasing 1.4-2 Log cycles of Lactobacillus acidophilus La-5 and Bifidobacterium animalis BB12. 30-50% α-glucosidase inhibition was observed in ultrasound, Flavourzyme and Cellulase extracts. Flavourzyme and Cellulase extracts are richer in macro (Mg, K and P) and micro elements (Zn, Mn and Fe) in comparison to mushroom. Monosaccharides content and profile varied slightly among both extracts with predominance of glucose, galactose and mannose with no uronic acids detection; Flavourzyme extract reported higher free amino acids content. Presence of α and β-glycosidic structures such as glucans and glucan-protein complexes are among the polysaccharides found in both extracts.
CESAM - Centre for Environmental and Marine Studies & Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal. Electronic address: dinarodrigues@ua.pt.
Dina
D
Rodrigues
CESAM - Centre for Environmental and Marine Studies & Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal; ISEIT/Viseu, Instituto Piaget, Estrada do Alto do Gaio, Galifonge, 3515-776 Lordosa, Viseu, Portugal.
Ana C
AC
Freitas
Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquitecto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
Sérgio
S
Sousa
Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquitecto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
Manuela
M
Amorim
Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquitecto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
Marta W
MW
Vasconcelos
CESAM - Centre for Environmental and Marine Studies & Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
João P
JP
da Costa
QOPNA - Organic Chemistry, Natural Products and Food Stuffs Research Unit & Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Artur M S
AM
Silva
CESAM - Centre for Environmental and Marine Studies & Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Teresa A P
TA
Rocha-Santos
CESAM - Centre for Environmental and Marine Studies & Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Armando C
AC
Duarte
Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquitecto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
Ana M P
AM
Gomes
D002480:Cellulase / Q000378:metabolism; D005936:Glucans / Q000032:analysis; D065089:Glycoside Hydrolase Inhibitors / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D009005:Monosaccharides / Q000032:analysis; D055368:Pholiota / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis
D005936:Glucans; D065089:Glycoside Hydrolase Inhibitors; D009005:Monosaccharides; D011134:Polysaccharides; D002480:Cellulase
Antidiabetic activity; Chemical characterization; Enzymatic- and ultrasound-assisted extraction; Pholiota nameko; Prebiotic activity; Structural characterization