Characterization of IL-17AA and IL-17FF in rheumatoid arthritis and multiple sclerosis.
OBJECTIVE
IL-17 is thought to play a prominent role in immune disorders. Sensitive and specific IL-17AA and IL-17FF assays were developed and used to determine levels in serum and cerebrospinal fluid (CSF) from patients with rheumatoid arthritis and relapsing remitting multiple sclerosis (RRMS).


RESULTS
Qualified assays detected IL-17AA and IL-17FF in healthy and disease samples. Serum IL-17AA was significantly higher in rheumatoid arthritis and RRMS as compared with normal healthy subjects. IL-17AA was also elevated in RRMS CSF as compared with normal healthy subjects; although correlation was observed between serum levels of the two isoforms, no correlation was detected between serum and CSF levels.


CONCLUSIONS
Reliable determination of IL-17 isoforms in the systemic and CNS compartments sheds light on the involvement of IL-17AA and IL-17FF in autoimmunity.
Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
Cheryl
C
Schofield
Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
Saloumeh K
SK
Fischer
Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
Michael J
MJ
Townsend
Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
Sofia
S
Mosesova
Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
Kun
K
Peng
Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
Alvernia Francesca
AF
Setiadi
Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
An
A
Song
Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
Amos
A
Baruch
D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000134:cerebrospinal fluid; D006801:Humans; D007118:Immunoassay / Q000379:methods; D020381:Interleukin-17 / Q000097:blood* / Q000134:cerebrospinal fluid; D057230:Limit of Detection; D009103:Multiple Sclerosis / Q000097:blood* / Q000134:cerebrospinal fluid; D012008:Recurrence
C508717:IL17A protein, human; C440021:IL17F protein, human; D020381:Interleukin-17
CSF; IL-17A; IL-17F; multiple sclerosis; rheumatoid arthritis; serum