Antimicrobial resistance of Listeria monocytogenes isolated from seafood and humans in Iran.
Fourteen Listeria monocytogenes isolates previously collected from seafood (n = 7) and human patients (n = 7) were studied for their antimicrobial susceptibility against eight common antimicrobials (ampicillin, penicillin, gentamicin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole, chloramphenicol, and cefotaxime). A high resistance level to ampicillin, cefotaxime (100%), and pencillin (57% in seafood isolates and 71.4% in clinical isolates) was observed in this study. However, all of the isolates were susceptible to trimethoprim-sulfamethoxazole, chloramphenicol, and tetracycline. Simultaneous resistance was identified in 4 clinical isolates (57.1%). Genotypic characterization of fish isolates (isolated from three fish species) was performed by pulsed-field gel electrophoresis (PFGE). A high diversity among fish isolates was observed. PFGE analyses distinguished the 4 isolates into 4 reproducible pulsotypes. There was no correlation between the antibiograms with pulsotypes. In conclusion, the resistance of seafood isolates to the antibiotics commonly used to treat listeriosis could be a potential health hazard for consumers.
Dept. of Seafood Science and Technology, Faculty of Fisheries and Environmental Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Esmail
E
Abdollahzadeh
Dept. of Seafood Science and Technology, Faculty of Fisheries and Environmental Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: mahdi_ojagh@yahoo.com.
Seyed Mahdi
SM
Ojagh
Dept. of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hedayat
H
Hosseini
Dept. of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran. Electronic address: eghaemi@yahoo.com.
Ezzat Allah
EA
Ghaemi
Dept. of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Gholamreza
G
Irajian
Dept. of Microbiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Masoud
M
Naghizadeh Heidarlo
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007492:Iran; D008089:Listeria monocytogenes / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D008088:Listeriosis / Q000382:microbiology*; D058889:Molecular Typing; D017747:Seafood / Q000382:microbiology*
D000900:Anti-Bacterial Agents
Antimicrobial resistance; Fish; Listeria monocytogenes; PFGE