Rapid nuclear import of short nucleic acids.
Exogenous short-chain nucleic acids undergo rapid import into the nucleus. Fluorescence-labeled dT1-13 DNA microinjected into the cytoplasm domain of a HeLa cell was rapidly imported into the nucleus domain within 1min. This is much more rapid than what has been observed for intracellular diffusion of small molecules. In contrast, import of longer nucleic acids with a length of over 30nt into the nucleus was suppressed.
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Mai
M
Kitagawa
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan; Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Akimitsu
A
Okamoto
D001692:Biological Transport; D006367:HeLa Cells; D006801:Humans; D009696:Nucleic Acids / Q000378:metabolism*
D009696:Nucleic Acids
Cell; Fluorescence; Nuclear import; Nucleic acids