Expression of Cytochrome P450s in the Liver of Rats Administered with Socheongryong-tang, a Traditional Herbal Formula.
OBJECTIVE
The purpose of this study was to investigate the potential influences of Socheongryong-tang (SCRT) on the messenger ribonucleic acid (mRNA) and protein expression of cytochrome P450 (CYP450) in vivo.


METHODS
SCRT was orally administered to either male or female Sprague-Dawley rats once daily at doses of 0, 1000, 2000, or 5000 mg/kg/day for 13 weeks. The mRNA expression of CYP450s (CYP1A1, 1A2, 2B1/2, 2C11, 2E1, 3A1, 3A2, and 4A1) in liver tissues was measured by reverse transcription polymerase chain reaction. And then, the protein expression of CYP1A1 and CYP2B1/2 in liver tissues was analyzed by the Western blot.


RESULTS
We found no significant influence in the mRNA expression of hepatic CYP1A2, 2C11, 2E1, 3A1, 3A2, and 4A1 after repeated administration of SCRT for 13 weeks. By contrast, the mRNA and protein expression of hepatic CYP1A1 was increased by repeated SCRT treatment in male rats, but not in female rats. The mRNA and protein expression of hepatic CYP2B1/2 in both genders was increased by administration of SCRT.


CONCLUSIONS
A caution is needed when SCRT is co-administered with substrates of CYP2B1/2 for clinical usage. In case of male, an attention is also required when SCRT and drugs metabolized by CYP1A1 are taken together. Our findings provide information regarding the safety and effectiveness of SCRT when combined with conventional drugs.


CONCLUSIONS
Oral administration of Socheongryong-tang for 13 weeks did not affect the mRNA expression of hepatic CYP1A2, 2C11, 2E1, 3A1, 3A2, and 4A1In male rats, oral administration of Socheongryong-tang for 13 weeks induced the mRNA and protein expression of hepatic CYP1A1 and CYP2B1/2In female rats, oral administration of Socheongryong-tang for 13 weeks induced the mRNA and protein expression of hepatic CYP2B1/2. Abbreviations used: SCRT: Socheongryong-tang, CYP450: Cytochrome P450, HPLC: High performance liquid chromatography,
Reverse transcription polymerase chain reaction.
K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea.
Seong Eun
SE
Jin
K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea.
Hyekyung
H
Ha
K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea.
Chang-Seob
CS
Seo
K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea.
Hyeun-Kyoo
HK
Shin
KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea.
Soo-Jin
SJ
Jeong
Cytochrome P450; Socheongryong-tang; herb-drug interactions; metabolism; rat liver