Determination of Histamine in Silages Using Nanomaghemite Core (γ-Fe₂O₃)-Titanium Dioxide Shell Nanoparticles Off-Line Coupled with Ion Exchange Chromatography.
The presence of biogenic amines is a hallmark of degraded food and its products. Herein, we focused on the utilization of magnetic nanoparticles off-line coupled with ion exchange chromatography with post-column ninhydrin derivatization and Vis detection for histamine (Him) separation and detection. Primarily, we described the synthesis of magnetic nanoparticles with nanomaghemite core (γ-Fe₂O₃) functionalized with titanium dioxide and, then, applied these particles to specific isolation of Him. To obtain further insight into interactions between paramagnetic particles' (PMP) surface and Him, a scanning electron microscope was employed. It was shown that binding of histamine causes an increase of relative current response of deprotonated PMPs, which confirmed formation of Him-PMPs clusters. The recovery of the isolation showed that titanium dioxide-based particles were able to bind and preconcentrate Him with recovery exceeding 90%. Finally, we successfully carried out the analyses of real samples obtained from silage. We can conclude that our modified particles are suitable for Him isolation, and thus may serve as the first isolation step of Him from biological samples, as it is demonstrated on alfalfa seed variety Tereza silage.
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic. cernei.natalia3@gmail.com.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, 61600 Brno, Czech Republic. cernei.natalia3@gmail.com.
Natalia
N
Cernei
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic. lackova14@seznam.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, 61600 Brno, Czech Republic. lackova14@seznam.cz.
Zuzana
Z
Lackova
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic. R.Guran@email.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, 61600 Brno, Czech Republic. R.Guran@email.cz.
Roman
R
Guran
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic. d.hynek@email.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, 61600 Brno, Czech Republic. d.hynek@email.cz.
David
D
Hynek
Department of Animal Nutrition and Forage Production, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic. sklady@mendelu.cz.
Jiri
J
Skladanka
Department of Animal Nutrition and Forage Production, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic. pavel.horky@mendelu.cz.
Pavel
P
Horky
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic. zitkao@seznam.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, 61600 Brno, Czech Republic. zitkao@seznam.cz.
Ondrej
O
Zitka
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 61300 Brno, Czech Republic. vojtech.adam@mendelu.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, 61600 Brno, Czech Republic. vojtech.adam@mendelu.cz.
Vojtech
V
Adam
D001679:Biogenic Amines; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry; D006632:Histamine / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry*; D012820:Silage / Q000032:analysis*; D014025:Titanium / Q000737:chemistry
D001679:Biogenic Amines; D005290:Ferric Compounds; C009495:titanium dioxide; D006632:Histamine; D014025:Titanium
aminoacids; biogenic amines; magnetic-particles-based separation; plant; specific isolation