Nitric Oxide Releasing Coronary Stent: A New Approach Using Layer-by-Layer Coating and Liposomal Encapsulation.
The sustained or controlled release of nitric oxide (NO) can be the most promising approach for the suppression or prevention of restenosis and thrombosis caused by stent implantation. The aim of this study is to investigate the feasibility in the potential use of layer-by-layer (LBL) coating with a NO donor-containing liposomes to control the release rate of NO from a metallic stent. Microscopic observation and surface characterizations of LBL-modified stents demonstrate successful LBL coating with liposomes on a stent. Release profiles of NO show that the release rate is sustained up to 5 d. In vitro cell study demonstrates that NO release significantly enhances endothelial cell proliferation, whereas it markedly inhibits smooth muscle cell proliferation. Finally, in vivo study conducted with a porcine coronary injury model proves the therapeutic efficacy of the NO-releasing stents coated by liposomal LBL technique, supported by improved results in luminal healing, inflammation, and neointimal thickening except thrombo-resistant effect. As a result, all these results demonstrate that highly optimized release rate and therapeutic dose of NO can be achieved by LBL coating and liposomal encapsulation, followed by significantly efficacious outcome in vivo.
Center for Biomaterials, Korea Institute of Science and Technology, Hwarangro 14-gil 5, Seoungbuk-gu, Seoul, 136-791, Republic of Korea.|Department of Biomedical Engineering, Korea University of Science and Technology, 113 Gwahangno, Yuseong-gu, Daejeon, 305-333, Republic of Korea.
Mahmoud A
MA
Elnaggar
Center for Biomaterials, Korea Institute of Science and Technology, Hwarangro 14-gil 5, Seoungbuk-gu, Seoul, 136-791, Republic of Korea.|College of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Republic of Korea.
Seong Ho
SH
Seo
Center for Biomaterials, Korea Institute of Science and Technology, Hwarangro 14-gil 5, Seoungbuk-gu, Seoul, 136-791, Republic of Korea.
Samy
S
Gobaa
The Heart Center of Chonnam National University Hospital, 671 Jaebongro, Dong-gu, Gwangju, 501-757, Republic of Korea.
Kyung Seob
KS
Lim
The Heart Center of Chonnam National University Hospital, 671 Jaebongro, Dong-gu, Gwangju, 501-757, Republic of Korea.
In-Ho
IH
Bae
The Heart Center of Chonnam National University Hospital, 671 Jaebongro, Dong-gu, Gwangju, 501-757, Republic of Korea.
Myung Ho
MH
Jeong
Center for Biomaterials, Korea Institute of Science and Technology, Hwarangro 14-gil 5, Seoungbuk-gu, Seoul, 136-791, Republic of Korea.|Department of Biomedical Engineering, Korea University of Science and Technology, 113 Gwahangno, Yuseong-gu, Daejeon, 305-333, Republic of Korea.
Dong Keun
DK
Han
Center for Biomaterials, Korea Institute of Science and Technology, Hwarangro 14-gil 5, Seoungbuk-gu, Seoul, 136-791, Republic of Korea.|Department of Biomedical Engineering, Korea University of Science and Technology, 113 Gwahangno, Yuseong-gu, Daejeon, 305-333, Republic of Korea.
Yoon Ki
YK
Joung
D000327:Adsorption; D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003331:Coronary Vessels / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D005340:Fibrinogen / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D009603:Nitroso Compounds / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques; D015607:Stents; D034421:Sus scrofa
D020099:Coated Materials, Biocompatible; D008081:Liposomes; D009603:Nitroso Compounds; C094210:2,2'-(hydroxynitrosohydrazono)bis-ethanamine; D009569:Nitric Oxide; D005340:Fibrinogen
layer-by-layer; liposomes; nitric oxide; restenosis; stents