Hemato-Immunological Responses and Disease Resistance in Siberian Sturgeon Acipenser baerii Fed on a Supplemented Diet of Lactobacillus plantarum.
A feeding trial was conducted to investigate the effects of different levels of dietary Lactobacillus plantarum on hemato-immunological parameters and resistance against Streptococcus iniae infection in juvenile Siberian sturgeon Acipenser baerii. Fish (14.6 ± 2.3 g) were fed three experimental diets prepared by supplementing a basal diet with L. plantarum at different concentrations [1 × 107, 1 × 108and 1 × 109colony-forming units (cfu) g-1] and a control (non-supplemented basal) diet for 8 weeks. Innate immune responses (immunoglobulin (Ig), alternative complement activity (ACH50) and lysozyme activity) were significantly higher in fish fed the 1 × 108and 1 × 109cfu g-1L. plantarum diet compared to the other groups (P < 0.05). Furthermore, fish fed on various levels of L. plantarum significantly showed higher red blood cell (RBC), hemoglobin (Hb), white blood cell (WBC) and monocyte compared to those of the control group (P < 0.05). At the end of the feeding experiment, some fish were challenged with S. iniae to quantify the level of disease resistance. The mortality after S. iniae challenge was decreased in fish fed a probiotic. These results indicated that dietary supplementation of L. plantarum improved immune response and disease resistance of Siberian sturgeon juvenile.
Caspian Sea International Sturgeon Research Institute, Rasht, Iran.
Moheb Ali
MA
Pourgholam
Fisheries Department, Faculty of Natural Resources, Islamic Azad University, Lahijan Branch, Lahijan, Iran.
Hossein
H
Khara
Ecology Research Institute of the Caspian Sea, Sari, Iran.
Reza
R
Safari
Caspian Sea International Sturgeon Research Institute, Rasht, Iran.
Mohammad Ali Yazdani
MA
Sadati
Fisheries Department, Faculty of Natural Resources, Urmia University, Urmia, Iran. msaramli@gmail.com.
Mohammad Sadegh
MS
Aramli
D000821:Animal Feed / Q000032:analysis / Q000382:microbiology; D000818:Animals; D001772:Blood Cell Count; D019587:Dietary Supplements / Q000032:analysis / Q000382:microbiology; D060467:Disease Resistance; D005393:Fish Diseases / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D005399:Fishes / Q000276:immunology / Q000382:microbiology; D006454:Hemoglobins / Q000378:metabolism; D007113:Immunity, Innate; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D019936:Probiotics / Q000008:administration & dosage*; D012800:Siberia; D013290:Streptococcal Infections / Q000097:blood / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D000070058:Streptococcus iniae / Q000502:physiology
D006454:Hemoglobins
Hematology parameters; Immunology parameters; Probiotic lactobacilli; Streptococcus iniae; Sturgeon