Exercise-induced expression of monocarboxylate transporter 2 in the cerebellum and its contribution to motor performance.
Monocarboxylate transporter 2 (MCT2) is an important component of the lactate transport system in neurons of the adult brain. Purkinje cells in the cerebellum have been shown to have high levels of MCT2, suggesting that this protein has a key function in energy metabolism and neuronal activities in these cells. However, it is not known whether inhibition of lactate transport via MCT2 in the cerebellum affects motor performance. To address this question, we examined motor performance in mice following the inhibition of lactate transport via MCT2 in the cerebellum using α-cyano-4-hydroxycinnamate (4-CIN). 4-CIN or saline was injected into the subarachnoidal space of the cerebellum of mice and motor performance was analyzed by a rotarod test both before and after injection. 4-CIN injection reduced retention time in the rotarod test by approximately 80% at 1h post-injection compared with pre-injection. No effect was observed at 2h post-injection or in mice treated with the vehicle control. Because we observed that MCT2 plays an important role in motor performance, we next investigated the effects of acute exercise on MCT2 transcription and protein levels in mice sampled pre-exercise and at 0 and 5h after 2h of treadmill running. We found a significant increase in MCT2 mRNA levels, but not of protein levels, in the cerebellum at 5h after exercise. Our results indicate that lactate transport via MCT2 in the cerebellum may play an important role in motor performance and that exercise can increase MCT2 expression at the transcriptional level.
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Daisuke
D
Hoshino
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Susumu
S
Setogawa
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Yu
Y
Kitaoka
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Hiroyuki
H
Masuda
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Yuki
Y
Tamura
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Hideo
H
Hatta
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan. Electronic address: dai-y@idaten.c.u-tokyo.ac.jp.
Dai
D
Yanagihara
D000818:Animals; D001692:Biological Transport; D002531:Cerebellum / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D003373:Coumaric Acids / Q000494:pharmacology; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D008813:Mice, Inbred ICR; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism*; D009043:Motor Activity; D010805:Physical Conditioning, Animal; D012333:RNA, Messenger / Q000378:metabolism; D027981:Symporters / Q000378:metabolism; D000099233:Monocarboxylate Transport Protein 1
D003373:Coumaric Acids; D019344:Lactic Acid; D027501:Monocarboxylic Acid Transporters; D012333:RNA, Messenger; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1; C490426:Slc16a7 protein, mouse; C007175:alpha-cyano-4-hydroxycinnamate
Cerebellum; Exercise; Lactate transport; Monocarboxylate transporter