Proteomic analysis of extracellular vesicles derived from Propionibacterium acnes.
Extracellular vesicle (EV) has been reported to conduct critical pathophysiological functions as an emerging mode of communication in bacteria. Recently, Propionibacterium acnes, an anaerobic Gram-positive human commensal found in the skin and gastrointestinal tract, has drawn increasing attention as an underestimated pathogen in a variety of diseases.



For the comprehensive understanding of P. acnes, here we report the isolation of P. acnes EVs for the first time and identification of 252 vesicular proteins with high confidence using triplicate LC-MS/MS analyses.



Comprehensive proteomic profiling reveals that P. acnes EVs harbor various proteins involved in biochemical processes, antibiotic resistance, bacterial competition, cell adherence, virulence, and immunogenicity.



We believe that this report will provide valuable information for investigating the biological role of P. acnes EVs and effective targets for developing clinical applications against P. acnes.
Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Nam-Gu, Pohang, Gyeongbuk, Republic of Korea.
Jinseong
J
Jeon
Department of Applied Chemistry, College of Applied Science, Kyung Hee University, Yongin, Gyeonggi, Republic of Korea.
Hyuck Jun
HJ
Mok
Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Nam-Gu, Pohang, Gyeongbuk, Republic of Korea.
Youngwoo
Y
Choi
Department of Applied Chemistry, College of Applied Science, Kyung Hee University, Yongin, Gyeonggi, Republic of Korea.
Seung Cheol
SC
Park
Department of Chemistry, Pohang University of Science and Technology (POSTECH), Nam-Gu, Pohang, Gyeongbuk, Republic of Korea.
Hunho
H
Jo
Department of Chemistry, Pohang University of Science and Technology (POSTECH), Nam-Gu, Pohang, Gyeongbuk, Republic of Korea.
Jin
J
Her
Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Nam-Gu, Pohang, Gyeongbuk, Republic of Korea.
Jin-Kwan
JK
Han
MD Healthcare, Mapo-gu, Seoul, Republic of Korea.
Yoon-Keun
YK
Kim
Department of Applied Chemistry, College of Applied Science, Kyung Hee University, Yongin, Gyeonggi, Republic of Korea.
Kwang Pyo
KP
Kim
Department of Chemistry, Pohang University of Science and Technology (POSTECH), Nam-Gu, Pohang, Gyeongbuk, Republic of Korea.
Changill
C
Ban
D002851:Chromatography, High Pressure Liquid; D000067493:Dynamic Light Scattering; D000067128:Extracellular Vesicles / Q000378:metabolism*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology / Q000473:pathology; D006801:Humans; D046529:Microscopy, Electron, Transmission; D011425:Propionibacterium acnes / Q000302:isolation & purification / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
D020543:Proteome
Extracellular vesicle; LC-MS/MS; Microbiology; Propionibacterium acnes; Theranostics