Statistical optimization of exopolysaccharide production by Lactobacillus plantarum NTMI05 and NTMI20.
In this study, 27 strains of Lactic acid bacteria (LAB) were isolated and identified from different milk sources. All the isolates were biochemically characterized and screened for their ability to produce exopolysaccharides (EPS), among which two isolates namely Lactobacillus plantarum NTMI05 (197mg/L) and Lactobacillus plantarum NTMI20 (187mg/L) showed higher EPS production. Both the isolates were molecular characterized and tested for their probiotic properties. The chemical composition of EPS from L. plantarum NTMI05 and NTMI20 revealed the presence of 95.45% and 92.35% carbohydrates, 14±0.1and 11±0.15mg/L lactic acid, 10.5±0.2 and 9±0.1mg/mL of reducing sugar, respectively. HPLC analysis showed galactose at the retention time of 2.29.The maximum EPS yield was optimized for the media components like glucose (20g/L), yeast extract (25g/L) and ammonium sulphate (2g/L) using Central Composite Design and Response Surface Methodology (RSM). Under optimum conditions the predicted maximum EPS production was 0.891g/L, 0.797g/L, while the actual experimental value was 0.956g/L and 0.827g/L for L. plantarum NTMI05 and NTMI20, respectively. The antioxidant capacity was also evaluated by DPPH and reducing power assay proving the potentiality of these organisms in food and dairy industries.
Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India.
Mohamed Yousuff Mohamed
MY
Imran
Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India; P.G. and Research Department of Microbiology, Jamal Mohamed College (Autonomous), Tiruchirappalli 620 020, Tamilnadu, India.
Nazar
N
Reehana
Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India.
K Arumugam
KA
Jayaraj
Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India.
Abdul Azees Parveez
AA
Ahamed
Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India.
Dharmadurai
D
Dhanasekaran
Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Nooruddin
N
Thajuddin
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Naiyf S
NS
Alharbi
Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India. Electronic address: drgm@bdu.ac.in.
Gangatharan
G
Muralitharan
D000975:Antioxidants / Q000737:chemistry*; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry; D019936:Probiotics / Q000737:chemistry*
D000975:Antioxidants; D011135:Polysaccharides, Bacterial
Central composite design; Exopolysaccharides; Lactic acid bacteria; Lactobacillus plantarum; Probiotic; Response surface methodology