Effects of polydeoxyribonucleotides (PDRN) on wound healing: Electric cell-substrate impedance sensing (ECIS).
Polydeoxyribonucleotides (PDRN) have been explored as an effective treatment for tissue repair in peripheral artery occlusive disease, diabetic foot ulcers, and eye lotion. We report on the effect of polydeoxyribonucleotides (PDRN) on wound healing by using the electric cell-substrate impedance sensing (ECIS) system and viability testing. Human osteoblasts (U2OS) and primary human dermal fibroblasts (HDF) were used to study the effect of PDRN on migration and proliferation. ECIS allowed the creation of a wound by applying high current, and then monitoring the healing process by measuring impedance in real time. The traditional culture-insert gap-closure migration assay was performed and compared with the ECIS wound assay. PDRN-treated U2OS and HDF cells affected cell motilities to wounding site. Viability test results show that HDF and U2OS proliferation depended on PDRN concentration. Based on the results, a PDRN compound can be useful in wound healing associated with bone and skin.
FIT BEST Laboratory, Department of Chemical, Biological, and Bio Engineering, North Carolina A&T State University, Greensboro, NC 27411, USA; NSF Engineering Research Center for Revolutionizing Metallic Biomaterials, North Carolina A&T State University, Greensboro, NC 27411, USA.
Youngmi
Y
Koo
FIT BEST Laboratory, Department of Chemical, Biological, and Bio Engineering, North Carolina A&T State University, Greensboro, NC 27411, USA; NSF Engineering Research Center for Revolutionizing Metallic Biomaterials, North Carolina A&T State University, Greensboro, NC 27411, USA. Electronic address: yyun@ncat.edu.
Yeoheung
Y
Yun
D000328:Adult; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D020405:Dermis / Q000473:pathology; D058266:Dielectric Spectroscopy; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D006801:Humans; D008839:Microelectrodes; D011089:Polydeoxyribonucleotides / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D011089:Polydeoxyribonucleotides
Drug screening; Electric cell-substrate impedance sensing (ECIS); Fibroblast; Osteoblast; Polydeoxyribonucleotide (PDRN)