Porous graphene oxide nanostructure as an excellent scaffold for label-free electrochemical biosensor: Detection of cardiac troponin I.
Herein, we report the fabrication of a novel label-free impedimetric biosensor employing porous graphene oxide (PrGO) nanostructures for the specific detection of cardiac troponin-I (cTnI) to establish the myocardial infarction (MI). This nano-immunosensor demonstrates an outstanding selectivity and high sensitivity towards the human-cTnI analyte. An excellent detection limit of 0.07ngmL(-1) and dynamic linear range of 0.1-10ngmL(-1) were calculated for anti-cTnI/PrGO/GC. Finally, this biosensor was employed to check the concentration of the MI biomarker in real clinical samples and the results are in good agreement with standard enzyme-linked fluorescence assay (ELFA) method.
Department of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan 45137-66731, Iran. Electronic address: habibkazemi@iasbs.ac.ir.
Sayed Habib
SH
Kazemi
Department of Medical Biotechnology and Nanotechnology, School of Medicine, Zanjan University of Medical Science, Zanjan, P.O. Box: 14115-111, Iran.
Elham
E
Ghodsi
Department of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan 45137-66731, Iran.
Siamak
S
Abdollahi
Department of Medical Biotechnology and Nanotechnology, School of Medicine, Zanjan University of Medical Science, Zanjan, P.O. Box: 14115-111, Iran; Cancer Gene Therapy Research Center, Zanjan University of Medical Science, Zanjan, Iran. Electronic address: nadrisamad@zums.ac.ir.
Samad
S
Nadri
D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000295:instrumentation; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D009203:Myocardial Infarction / Q000175:diagnosis; D049329:Nanostructures / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D016062:Porosity; D019210:Troponin I / Q000032:analysis*
D015415:Biomarkers; D010087:Oxides; D019210:Troponin I; D006108:Graphite
Cardiac troponin-I (cTnI); Electrochemical impedance; Glassy carbon electrode; Immunosensor; Nanostructure; Porous graphene oxide