[Macrophages promote the migration of neural stem cells into mouse spinal cord injury site].
Objective To explore the role of macrophages in the migration of neural stem cells (NSCs) in vivo and in vitro . Methods NSCs with green fluorescent protein (GFP) were isolated from GFP transgenic mice and the immunofluorescence cytochemical staining of nestin was used to identify NSCs. After spinal cord injury was induced, the tissue level of macrophage chemotactic protein-1 (MCP-1) mRNA was detected using quantitative real time PCR. The migration of GFP-NSCs was investigated 1 week after GFP-NSCs were injected into both sides of the damaged area. The effect of macrophage on the migration of NSCs in vitro was tested by Transwell(TM) system and the content of MCP-1 was detected by ELISA. Results NSCs highly expressed nestin. Compared with the control group, the level of MCP-1 mRNA significantly increased in the spinal cord injury group. The NSCs which were injected into the spinal cord migrated into the center of the injured site where F4/80 was highly expressed. Macrophages significantly increased the number of migrating NSCs in vitro and the secretion of MCP-1. Conclusion Macrophages induce NSC migrating into the spinal cord injury site possibly through promoting the secretion of MCP-1.
Department of Orthopaedics, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
Zhijian
Z
Cheng
Deparment of Critical Care Medicine, First People's Hospital of Xianyany City, Xianyang 710021, China.
Wen
W
Zhu
Department of Orthopaedics, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
Haopeng
H
Li
Department of Orthopaedics, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China. *Corresponding author, E-mail: xijing_h@vip.tom.com.
Xijing
X
He
D000818:Animals; D002465:Cell Movement; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology*; D013119:Spinal Cord Injuries / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D013234:Stem Cells / Q000166:cytology*
C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2