Hemostatic efficacy of local chitosan linear polymer granule in an experimental sheep model with severe bleeding of arteria and vena femoralis.
BACKGROUND
The aim of the present study was to evaluate the hemostatic effect of chitosan linear polymer in a sheep model with femoral bleeding.


METHODS
Following induction of anesthesia and intubation of sheep, groin injury was induced to initiate hemorrhage. Animals were randomly assigned to study and control groups. In the control group, absorbent pads were packed on the wound, and pressure was supplied by a weight placed over the dressing. In the study group, chitosan linear polymer was poured onto the bleeding site; absorbent pads and pressure were applied in the same manner. At 5-min intervals, bleeding was evaluated. Primary endpoint was time to hemostasis.


RESULTS
Bleeding had stopped by the 1st interval in 5 members of the study group, and by the 2nd interval in 1 member. One sheep was excluded. The bleeding stopped after the 1st interval in 1 member of the control group and after the 2nd interval in 4 members. Bleeding stopped in 2 cases following ligation of the bleeding vessel. Hemostasis was achieved earlier in the study group, compared to the control group, and the difference was statistically significant.


CONCLUSIONS
Hemostasis was achieved earlier following application of chitosan linear polymer.
Department of Emergency Medicine, Dokuz Eylül University Faculty of Medicine, İzmir-Turkey. gurkan.ersoy@ymail.com.
Gürkan
G
Ersoy
Family Physician (Private Doctor), İzmir-Turkey.
Ülkümen
Ü
Rodoplu
Department of Laboratory Animals Science, Dokuz Eylül University Faculty of Medicine, İzmir-Turkey.
Osman
O
Yılmaz
Department of Anesthesiology and Reanimation, Dokuz Eylül University Faculty of Medicine, İzmir-Turkey.
Necati
N
Gökmen
Department of Anesthesiology, Denizli State Hospital, Denizli-Turkey.
Alper
A
Doğan
Department of Emergency Medicine, İstanbul Training and Research Hospital, İstanbul-Turkey.
Özgür
Ö
Dikme
Department of Emergency Medicine, Medical Park Hospital, İzmir-Turkey.
Aslı
A
Aydınoğlu
Maritime Management, Salvage Master (Vessel Recovery, Wreck Removal for Ship) and Oil Spill Specialist, Oceangoing Watchkeeper, İstanbul-Turkey.
Okyanus
O
Orhon
D000818:Animals; D048271:Chitosan; D004195:Disease Models, Animal; D005260:Female; D005263:Femoral Artery / Q000293:injuries / Q000601:surgery; D005268:Femoral Vein / Q000293:injuries / Q000601:surgery; D006119:Groin / Q000293:injuries / Q000601:surgery; D006470:Hemorrhage / Q000628:therapy*; D006489:Hemostatic Techniques; D006490:Hemostatics; D008297:Male; D009779:Occlusive Dressings; D011108:Polymers; D012756:Sheep; D014947:Wounds and Injuries / Q000628:therapy
D006490:Hemostatics; D011108:Polymers; D048271:Chitosan