Controlled formaldehyde fixation of fibronectin layers for expansion of mesenchymal stem cells.
Extracellular cell matrices deposited by cells stimulate cell proliferation. However, their generation is cumbersome and time consuming. We show here that controlled fixation of fibronectin layers after coating culture vessels significantly enhances expansion of murine and human mesenchymal stem cells (MSCs) and, to a lesser extent, primary fibroblasts. In contrast, fibronection fixation did not stimulate proliferation of established cancer cell lines. Fixed vitronectin or collagen IV layers also enhanced proliferation of murine MSCs. Thus, controlled formaldehyde fixation of layers formed by fibronectin or some other extracellular matrix components represents a simple and reproducible way to enhance proliferation of primary cells.
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow 119991, Russia.
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NV
Andreeva
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow 119991, Russia.
O G
OG
Leonova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow 119991, Russia.
V I
VI
Popenko
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow 119991, Russia. Electronic address: abelyavs@yahoo.com.
A V
AV
Belyavsky
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D024141:Collagen Type IV / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D005557:Formaldehyde / Q000737:chemistry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice
D024141:Collagen Type IV; D005353:Fibronectins; D005557:Formaldehyde
Collagen IV; Fibronectin; Fixation; Formaldehyde; Mesenchymal stem cells; Vitronectin