Ex Vivo Evaluation of Thymus daenensis as an Antioxidant and Antibacterial Medicinal Herb.
Herbal medicines are defined as traditionally used natural products. The current study in an attempt try to investigate the antibacterial activity on extended spectrum beta-lactamases (ESBL), methalo beta-lactamases (MBL) producing gram negative bacteria and methicillin resistantStaphylococcus aureus(MRSA) and subsequently, to determine the antioxidant activity ofThymus daenensis. For this reasons, firstly cytotoxicity ofT. daenensiswas determined by MTT assay. Then, essential oil was subjected for antibacterial and antioxidant activity. Our results demonstrated that 15 mg/ml concentration ofT. daenensisinhibited bothP. aeruginosaproducing MBL andE.coliproducing ESBL, while this value was 25 mg/ml concentration for MRSA inhibition. The association between phenolic compound and antioxidant activity was found for the ABTS•+method (43.52%) in the lowest level, while, for FRAD and DPPH•methods the opposite story occurred (70.5% correlation for DPPH•and 50.9% for FRAD). Our findings suggested thatT. daenensishas a potential herbal medicine that should be considered as an antibacterial and antioxidant with very low toxicity.
 Department of Horticultural Sciences, College of Agriculture, University of Ilam, Ilam, Iran.
M
M
Saidi
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
N
N
Sadeghifard
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
H
H
Kazemian
Department of Medical Microbiology and parasitology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Malaysia.
Z
Z
Sekawi
Department of Internal Medicine, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
B
B
Badakhsh
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
S
S
Friadian
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
S
S
Ghafourian
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000379:methods; D009822:Oils, Volatile / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D046930:Thymus Plant / Q000737:chemistry*; D014709:Vero Cells
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D009822:Oils, Volatile; D010636:Phenols; D010936:Plant Extracts