Demineralizing potential of dental biofilm added with Candida albicans and Candida parapsilosis isolated from preschool children with and without caries.
This study aimed to investigate the demineralizing potential of dental biofilm added of Candida albicans (CA) and Candida parapsilosis (CP), isolated from preschoolers with and without caries. Bovine enamel blocks (n = 48), with initial hardness = 341.50 ± 21,83 kg/mm2were fixed in 24 well plates containing culture media. A pool of children saliva (PHS) was the inoculum for biofilm formation in the presence or absence of isolated CA or CP in accordance with each group (G n = 8): G1 - PHS; G2 - PHS + CA isolated from children with caries; G3 - PHS + CP isolated from children with caries; G4 - PHS + CA isolated from children without caries; G5 - PHS + CP isolated from children without caries; and G6 - blank control. The plates were incubated at 37 °C for 5 days, with daily changes of culture media. The microhardness loss percentage (MHL%) of the blocks was calculated, taking in account the hardness values before and after the experiment. Dental biofilm became more cariogenic, independently of the isolated Candida species. The highest MHL% was observed in G4 (85.90 ± 8.72%) and G5 (86.13 ± 6.74%) compared to the others (p < 0.001): G1 (34.30 ± 14,30%) < G2 (59.40 ± 10.56%) and G3 (65.80 ± 6.36%) < G6 (13.68 ± 4.86%) (p < 0.001). C. albicans and C. parapsilosis isolates induced the demineralization of the dental enamel.
Departamento de Odontopediatria e Ortodontia, da Faculdade de Odontologia da Universidade Federal do Rio de Janeiro, Brazil.
Thayse
T
Caroline de Abreu Brandi
Departamento de Clínica e Odontopediatria, da Faculdade de Odontologia da Universidade Federal Fluminense, Niterói, Brazil.
Maristela Barbosa
MB
Portela
Departamento de Odontopediatria e Ortodontia, da Faculdade de Odontologia da Universidade Federal do Rio de Janeiro, Brazil.
Paula Moraes
PM
Lima
Departamento de Odontopediatria e Ortodontia, da Faculdade de Odontologia da Universidade Federal do Rio de Janeiro, Brazil.
Gloria Fernanda Barbosa de Araújo
GFBA
Castro
Departamento de Odontopediatria e Ortodontia, da Faculdade de Odontologia da Universidade Federal do Rio de Janeiro, Brazil.
Lucianne Cople
LC
Maia
Departamento de Odontopediatria e Ortodontia, da Faculdade de Odontologia da Universidade Federal do Rio de Janeiro, Brazil. Electronic address: andreagantonio@yahoo.com.br.
Andréa
A
Fonseca-Gonçalves
D000818:Animals; D018441:Biofilms / Q000254:growth & development*; D002175:Candida / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D002417:Cattle; D002675:Child, Preschool; D003731:Dental Caries / Q000382:microbiology; D003743:Dental Enamel / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D009055:Mouth / Q000382:microbiology; D013696:Temperature; D013995:Time; D017001:Tooth Demineralization
Candida albicans; Candida parapsilosis; Children; Dental caries; Dental plaque; Microhardness