Inhibition of osteoblast differentiation by aluminum trichloride exposure is associated with inhibition of BMP-2/Smad pathway component expression.
Bone morphogenetic protein-2 (BMP-2)/Smad signaling pathway plays an important role in regulating osteoblast (OB) differentiation. OB differentiation is a key process of bone formation. Aluminum (Al) exposure inhibits bone formation and causes Al-induced bone disease. However, the mechanism is not fully understood. To investigate whether BMP-2/Smad signaling pathway is associated with OB differentiation in aluminum trichloride (AlCl3)-treated OBs, the primary rat OBs were cultured and exposed to 0 (control group, CG), 1/40 IC50(low-dose group, LG), 1/20 IC50(mid-dose group, MG), and 1/10 IC50(high-dose group, HG) of AlCl3for 24 h, respectively. We found that the expressions of OB differentiation markers (Runx-2, Osterix and ALP) and BMP-2/Smad signaling pathway components (BMP-2, BMPR-IA, p-BMPR-IA, BMPR-II, p-Smad1/5/8 and p-Smad1/5/8/4) were all decreased in AlCl3-treated OBs compared with the CG. These results indicated that inhibition of OB differentiation by AlCl3was associated with inhibition of BMP-2/Smad pathway component expression. Our findings provide a novel insight into the mechanism of AlCl3-induced bone disease.
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Xu
X
Yang
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Hui
H
Huo
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Chunyu
C
Xiu
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Miao
M
Song
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Yanfei
Y
Han
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address: liyanfei@neau.edu.cn.
Yanfei
Y
Li
Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China. Electronic address: zhuyanzhu@caas.cn.
Yanzhu
Y
Zhu
D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D001252:Astringents / Q000494:pharmacology; D015153:Blotting, Western; D055396:Bone Morphogenetic Protein 2 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D002712:Chlorides / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D047468:Immunoprecipitation; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D051785:Smad Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
D017607:Aluminum Compounds; D001252:Astringents; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D002712:Chlorides; D012333:RNA, Messenger; D051785:Smad Proteins; D000077410:Aluminum Chloride
Aluminum trichloride; BMP-2/Smad signaling pathway; Osteoblast differentiation