The Ca2+-activated Cl- channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons.
The Ca(2+)-activated Cl(-) channel TMEM16B is highly expressed in the cilia of olfactory sensory neurons (OSNs). Although a large portion of the odor-evoked transduction current is carried by Ca(2+)-activated Cl(-) channels, their role in olfaction is still controversial. A previous report (Billig et al. 2011. Nat. Neurosci. http://dx.doi.org/10.1038/nn.2821) showed that disruption of the TMEM16b/Ano2 gene in mice abolished Ca(2+)-activated Cl(-) currents in OSNs but did not produce any major change in olfactory behavior. Here we readdress the role of TMEM16B in olfaction and show that TMEM16B knockout (KO) mice have behavioral deficits in odor-guided food-finding ability. Moreover, as the role of TMEM16B in action potential (AP) firing has not yet been studied, we use electrophysiological recording methods to measure the firing activity of OSNs. Suction electrode recordings from isolated olfactory neurons and on-cell loose-patch recordings from dendritic knobs of neurons in the olfactory epithelium show that randomly selected neurons from TMEM16B KO mice respond to stimulation with increased firing activity than those from wild-type (WT) mice. Because OSNs express different odorant receptors (ORs), we restrict variability by using a mouse line that expresses a GFP-tagged I7 OR, which is known to be activated by heptanal. In response to heptanal, we measure dramatic changes in the firing pattern of I7-expressing neurons from TMEM16B KO mice compared with WT: responses are prolonged and display a higher number of APs. Moreover, lack of TMEM16B causes a markedly reduced basal spiking activity in I7-expressing neurons, together with an alteration of axonal targeting to the olfactory bulb, leading to the appearance of supernumerary I7 glomeruli. Thus, TMEM16B controls AP firing and ensures correct glomerular targeting of OSNs expressing I7. Altogether, these results show that TMEM16B does have a relevant role in normal olfaction.
Neurobiology Group, International School for Advanced Studies (SISSA), 34136 Trieste, Italy.
Gianluca
G
Pietra
Monell Chemical Senses Center, Philadelphia, PA 19104.
Michele
0000-0002-3955-4663
M
Dibattista
Neurobiology Group, International School for Advanced Studies (SISSA), 34136 Trieste, Italy boccaccio@ge.ibf.cnr.it menini@sissa.it jreisert@monell.org.
Anna
0000-0001-9304-0412
A
Menini
Monell Chemical Senses Center, Philadelphia, PA 19104 boccaccio@ge.ibf.cnr.it menini@sissa.it jreisert@monell.org.
Johannes
J
Reisert
Institute of Biophysics, National Research Council (CNR), 16149 Genova, Italy boccaccio@ge.ibf.cnr.it menini@sissa.it jreisert@monell.org.
Anna
0000-0002-6219-2285
A
Boccaccio
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D000075368:Anoctamins / Q000235:genetics / Q000378:metabolism*; D005247:Feeding Behavior; D005786:Gene Expression Regulation / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D018034:Olfactory Receptor Neurons / Q000502:physiology*; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism; D012903:Smell / Q000502:physiology
C532161:ANO2 protein, mouse; D000075368:Anoctamins; D018035:Receptors, Odorant