Complete genome sequencing and comparative analyses of broad-spectrum antimicrobial-producing Micromonospora sp. HK10.
Micromonospora genus produces >700 bioactive compounds of medical relevance. In spite of its ability to produce high number of bioactive compounds, no genome sequence is available with comprehensive secondary metabolite gene clusters analysis for anti-microbial producing Micromonospora strains. Thus, here we contribute the full genome sequence of Micromonospora sp. HK10 strain, which has high antibacterial activity against several important human pathogens like, Mycobacterium abscessus, Mycobacterium smegmatis, Bacillus subtillis, Staphylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella and Escherichia coli. We have generated whole genome sequence data of Micromonospora sp. HK10 strain using Illumina NexSeq 500 sequencing platform (2×150bp paired end library) and assembled it de novo. The sequencing of HK10 genome enables identification of various genetic clusters associated with known- and probably unknown- antimicrobial compounds, which can pave the way for new antimicrobial scaffolds.
Department of Biotechnology, CSIR-North East Institute of Science and Technology, Jorhat 785006, India.
Madhumita
M
Talukdar
Department of Biotechnology, CSIR-North East Institute of Science and Technology, Jorhat 785006, India.
Dhrubajyoti
D
Das
Department of Biotechnology, CSIR-North East Institute of Science and Technology, Jorhat 785006, India.
Chiranjeeta
C
Bora
Department of Biotechnology, CSIR-North East Institute of Science and Technology, Jorhat 785006, India.
Tarun Chandra
TC
Bora
Department of Biotechnology, CSIR-North East Institute of Science and Technology, Jorhat 785006, India.
Hari Prasanna
HP
Deka Boruah
Department of Biotechnology, CSIR-North East Institute of Science and Technology, Jorhat 785006, India; Academy of Scientific and Innovative Research, Rafi Marg, New Delhi 110001, India. Electronic address: 1010anil@gmail.com.
Anil Kumar
AK
Singh
D000700:Analgesics / Q000378:metabolism; D016680:Genome, Bacterial / Q000502:physiology*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008847:Micromonospora / Q000235:genetics* / Q000378:metabolism
D000700:Analgesics
Anti-microbial producing; Bacterial genomics; Micromonospora; Secondary metabolite