Laryngeal Cancer, HPV DNA vs E6/E7 mRNA Test: A Systematic Review.
OBJECTIVE
The reported range of involvement of human papillomavirus (HPV) in laryngeal squamous cell carcinoma (SCC) is wide because of the methods used to detect HPV.


METHODS
A computerized Medline study was carried out using the following as key words: "Papillomavirus Infections"[Mesh] and "Laryngeal Neoplasms"[Mesh].


METHODS
Studies that were included were written in English and reported results of HPV DNA with RNA in laryngeal SCC.


RESULTS
There were six reported HPV mRNA extraction. Among these studies, Lewis et al reported that out of the 31 cases analyzed, only 2 were HPV DNA+ and of these only 1 was mRNA HPV+ (3%). Halec et al reported 102 cases of which 32 were HPV DNA+ cases and of which only 6 were mRNA+ (5%). Chernock et al reported 76 cases of which 13 were HPV DNA+ cases and of which 4 were mRNA+ (5%). Masand et al reported 8 cases of which 1 was HPV DNA+ case and none was mRNA+. Gheit et al reported 43 cases of which 4 were HPV DNA+ cases and of which 2 were mRNA+ (4%). Castellsagné et al reported 1042 cases of which 59 were HPV DNA+ case and of which 51 were mRNA+ (4.8%) CONCLUSIONS: When determining the role of HPV in laryngeal SCC, evidence of HPV DNA warrants further examination for E6/E7 mRNA as simple assays such as p16 are nonspecific in laryngeal SCC. Further studies of HPV and its role in laryngeal SCC are warranted.
Department of Sensory Organs, "La Sapienza" University of Rome, Rome, Italy. Electronic address: Massimo.fusconi@libero.it.
Massimo
M
Fusconi
Department of Sensory Organs, "La Sapienza" University of Rome, Rome, Italy.
Flaminia
F
Campo
Department of Medical-Surgical Biotechnologies and Science, ENT, "La Sapienza" University of Rome, Latina, LT, Italy.
Andrea
A
Gallo
Department of Sensory Organs, "La Sapienza" University of Rome, Rome, Italy.
Giampietro
G
Zambetti
Department of Medical-Surgical Biotechnologies and Science, ENT, "La Sapienza" University of Rome, Latina, LT, Italy.
Salvatore
S
Martellucci
Institute of ENT, Catholic University of the Sacred Heart, Agostino Gemelli of Rome, Italy.
Annalisa
A
Seccia
Department of Sensory Organs, "La Sapienza" University of Rome, Rome, Italy.
Marco
M
de Vincentiis
D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000821:virology*; D002472:Cell Transformation, Viral; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000235:genetics*; D006258:Head and Neck Neoplasms / Q000175:diagnosis / Q000821:virology*; D061809:Human Papillomavirus DNA Tests; D006801:Humans; D017403:In Situ Hybridization; D007822:Laryngeal Neoplasms / Q000175:diagnosis / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins / Q000235:genetics*; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000821:virology*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D015203:Reproducibility of Results; D000077195:Squamous Cell Carcinoma of Head and Neck
D015344:DNA Probes, HPV; D004279:DNA, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; D012367:RNA, Viral
E6/E7 mRNA; HPV DNA; Human papillomavirus; Laryngeal squamous cell carcinoma; p16