Comparison of Urinary Total Proteins by Four Different Methods.
The total proteins in human urine have been compared by sulfosalicylic acid, sulfosalicylic acid with sodium sulphate and trichloroacetic acid methods with pyrogallol red molybdate method as there are no studies found quantifying imprecision and bias components. Fresh urine of 36 patients was analyzed by four methods. Imprecision and inaccuracy were determined by repeated analysis and method comparison studies using correlation plots, Bland and Altman, and Passing and Bablok regression analyses respectively. The coefficient of variation was 5.07 % for pyrogallol red molybdate; 6.84 % for sulfosalicylic acid; 3.97 % for sulfosalicylic acid with sodium sulphate and 5.93 % for trichloroacetic acid methods. Bland and Altman analysis showed a bias of 5.8, 1.7 and -5.4 for pyrogallol red molybdate versus sulfosalicylic acid, sulfosalicylic acid with sodium sulphate and trichloroacetic acid methods respectively. Passing and Bablok regression revealed a constant bias for pyrogallol red molybdate versus all turbidimetric methods but a proportional bias only with trichloroacetic acid method. Sulfosalicylic acid with sodium sulphate method is preferred to sulfosalicylic acid and trichloroacetic acid methods.
Department of Biochemistry, Mediciti Institute of Medical Sciences, SHARE INDIA, Ghanpur Village, Medchal Mandal, R.R.District, Hyderabad, Telangana India.
Padma
0000-0002-8329-4766
P
Yalamati
Department of Biochemistry, Mediciti Institute of Medical Sciences, SHARE INDIA, Ghanpur Village, Medchal Mandal, R.R.District, Hyderabad, Telangana India.
Madhu Latha
ML
Karra
Department of Biochemistry, Mediciti Institute of Medical Sciences, SHARE INDIA, Ghanpur Village, Medchal Mandal, R.R.District, Hyderabad, Telangana India.
Aparna V
AV
Bhongir
Bland and Altman analysis; Correlation; Dye-binding methods; Passing and Bablok regression analysis; Turbidimetric methods; Urinary protein assays