Application of Tissue Microarray Technology to Stem Cell Research.
There is virtually an unlimited number of possible Tissue Microarray (TMA) applications in basic and clinical research and ultimately in diagnostics. However, to assess the functional importance of novel markers, researchers very often turn to cell line model systems. The appropriate choice of a cell line is often a difficult task, but the use of cell microarray (CMA) technology enables a quick screening of several markers in cells of different origins, mimicking a genomic-scale analysis. In order to improve the morphological evaluations of the CMA slides we harvested the cells by conventional trypsinization, mechanical scraping and cells grown on coverslips. We show that mechanical scraping is a good evaluation method since keeps the real morphology very similar to those grown on coverslips. Immunofluorescence images are of higher quality, facilitating the reading of the biomarker cellular and subcellular localization. Here, we describe CMA technology in stem cell research.
UOS-IRGB-CNR, Via Fantoli 16/15, 20138, Milano, Italy. laspada.alberto@gmail.com.
Alberto
A
La Spada
IRCCS-Multimedica, Via Fantoli 16/15, 20138, Milano, Italy. barnaba.rainoldi@multimedica.it.
Barnaba
B
Rainoldi
Integrated Systems Engineering, Via Fantoli 16/15, 20138, Milano, Italy. andrea.deblasio@isenet.it.
Andrea
A
De Blasio
UOS-IRGB-CNR, Via Fantoli 16/15, 20138, Milano, Italy. ida.biunno@irgb.cnr.it.|IRCCS-Multimedica, Via Fantoli 16/15, 20138, Milano, Italy. ida.biunno@irgb.cnr.it.
Ida
I
Biunno
cell microArray (CMA); stem cells; tissue microArray (TMA)