Preparation and characterization of hydroxypropyl chitosan modified with collagen peptide.
Collagen peptide (COP) had been grafted to hydroxypropyl chitosan (HPCS) by using microbial transglutaminase (MTGase) as biocatalyst. HPCS was synthesized from chitosan and propylene oxide under alkali condition. The chemical structures of derivative were characterized by FT-IR and1H NMR spectroscopy. The process conditions were optimized from the aspects of the reaction time, the reaction temperature, the molar ratio of COP and the mass ratio of MTGase to HPCS. In this study, HPCS-COP could serve not only to reduce the loss of moisture but also to absorb the moisture, and the moisture absorption and moisture retention abilities were closely related to the degree of substitution (DS) values. In addition, with the DS and concentration increase of HPCS-COP, the radical scavenging activity increased in vitro antioxidant activity. Furthermore, the methyl thiazolyl tetrazolium assay (MTT) was applied to evaluate the biocompatibility of HPCS-COP, and the result indicated that HPCS-COP with the DS of 0.34 displayed pronounced cell viability at 50ppm. Therefore, the results suggest that HPCS-COP could be potential wound dressings for clinical applications.
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
Lihong
L
Fan
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
Shengqiong
S
Zou
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
Hongyu
H
Ge
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
Yao
Y
Xiao
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
Huigao
H
Wen
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
Han
H
Feng
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
Meng
M
Liu
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, China. Electronic address: niemin@whu.edu.cn.
Min
M
Nie
D065966:Absorption, Physicochemical; D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D007700:Kinetics; D051379:Mice; D041681:NIH 3T3 Cells; D010446:Peptide Fragments / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D013696:Temperature; D011503:Transglutaminases / Q000378:metabolism
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010446:Peptide Fragments; D010851:Picrates; D017665:Hydroxyl Radical; D003094:Collagen; D048271:Chitosan; C004931:1,1-diphenyl-2-picrylhydrazyl; D011503:Transglutaminases
Collagen peptide; Hydroxypropyl chitosan; Microbial transglutaminase; Wound dressing