A rapid method to estimate NADH regeneration rates in living cells.
We introduce a rapid whole cell assay for the estimation of NADH regeneration rates based on the fluorescent dye resazurin. A co-feed of formate and glucose, known to increase the intrinsic NADH regeneration rate of P. putida KT2440, was chosen as model system for the validation of this assay.
Institute of Applied Microbiology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.
Jannis
J
Kuepper
Institute of Applied Microbiology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.
Sebastian
S
Zobel
Institute of Applied Microbiology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany. Electronic address: Nick.wierckx@rwth-aachen.de.
Nick
N
Wierckx
Institute of Applied Microbiology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.
Lars M
LM
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D001426:Bacterial Proteins / Q000378:metabolism; D001669:Biochemical Phenomena; D002384:Catalysis; D004734:Energy Metabolism; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism; D008954:Models, Biological; D009243:NAD / Q000032:analysis* / Q000378:metabolism*; D010078:Oxazines; D010084:Oxidation-Reduction; D016958:Pseudomonas putida / Q000378:metabolism / Q000502:physiology; D012038:Regeneration; D014966:Xanthenes
D001426:Bacterial Proteins; D010078:Oxazines; D014966:Xanthenes; D009243:NAD; C005843:resazurin; D005947:Glucose
Formate; NADH; NADH regeneration rate; Pseudomonas putida; Redox biocatalysis