A Periodontal pathogen Porphyromonas gingivalis deteriorates Isoproterenol-Induced myocardial remodeling in mice.
Heart failure is a serious disease induced by several conditions, including hypertrophic cardiomyopathy. Although many reports suggest that there is an association between periodontal disease and cardiovascular disease, the mechanisms have yet to be elucidated. The purpose of this study was to clarify the relationship between periodontal disease and heart disease, especially in cardiac hypertrophy. We used C57BL/6J mice and implanted two types of subcutaneous chambers. First, we subcutaneously implanted a coil-shaped chamber into the back of a mouse. Porphyromonas gingivalis (P.g.), a major periodontal pathogen, was injected into the chamber. Then, an osmotic pump was implanted to infuse isoproterenol. Four weeks after the ISO infusion, we performed echocardiography and harvested the heart and blood. We measured the serum level of anti-P.g.-IgG using ELISA. The mRNA levels of several factors were measured using PCR. We found stronger cardiomyocyte hypertrophy in the ISO(+)/P.g.(+) mice compared with the ISO(+)/P.g.(-) mice. The total square of randomly selected cardiomyocytes was 23% larger in the ISO(+)/P.g.(+) mice than in the ISO(+)/P.g.(-) mice. We detected a higher level of mRNA expression in Toll-like receptor 2 and NADPH oxidase 4 in the ISO(+)/P.g.(-) mice compared with the control group. We revealed that a periodontal pathogen affected ISO-induced cardiac hypertrophy via oxidative stress.
Department of Periodontology, Tokyo Medical and Dental University, Tokyo, Japan.
Hiroki
H
Sato
Departments of Advanced Clinical Science and Therapeutics, The University of Tokyo, Tokyo, Japan.
Jun-Ichi
JI
Suzuki
Department of Periodontology, Tokyo Medical and Dental University, Tokyo, Japan.
Norio
N
Aoyama
Department of Cardiovascular Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Ryo
R
Watanabe
Department of Periodontology, Tokyo Medical and Dental University, Tokyo, Japan.
Makoto
M
Kaneko
Department of Periodontology, Tokyo Medical and Dental University, Tokyo, Japan.
Yuka
Y
Shiheido
Department of Periodontology, Tokyo Medical and Dental University, Tokyo, Japan.
Asuka
A
Yoshida
Departments of Advanced Clinical Science and Therapeutics, The University of Tokyo, Tokyo, Japan.
Kouji
K
Wakayama
Departments of Advanced Clinical Science and Therapeutics, The University of Tokyo, Tokyo, Japan.
Hidetoshi
H
Kumagai
Department of Cardiovascular Medicine, The University of Tokyo, Tokyo, Japan.
Yuichi
Y
Ikeda
Department of Cardiovascular Medicine, The University of Tokyo, Tokyo, Japan.
Hiroshi
H
Akazawa
Department of Cardiovascular Medicine, The University of Tokyo, Tokyo, Japan.
Issei
I
Komuro
Department of Cardiovascular Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Mitsuaki
M
Isobe
Department of Periodontology, Tokyo Medical and Dental University, Tokyo, Japan.
Yuichi
Y
Izumi
D000318:Adrenergic beta-Agonists; D000818:Animals; D006332:Cardiomegaly / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D006321:Heart / Q000187:drug effects* / Q000382:microbiology*; D007545:Isoproterenol / Q000494:pharmacology*; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000473:pathology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D016966:Porphyromonas gingivalis; D020257:Ventricular Remodeling / Q000187:drug effects*
D000318:Adrenergic beta-Agonists; D007545:Isoproterenol