Potential therapeutic application of small molecule with sulfonamide for chondrogenic differentiation and articular cartilage repair.
The restoration of damaged articular cartilage is a long-pursued goal in regenerative medicine. Chondrocyte-specific differentiation of mesenchymal stem cells (MSCs) may be an effective means of repairing damaged cartilage. We identified small molecule 6 with sulfonamide as an agent that promotes specific chondrogenic differentiation of human adipose-derived MSCs (hASCs). Unlike other chondrogenic differentiation media composed of various defined components, simply adding compound 6 into culture medium was sufficient to induce chondrogenesis in this study. In an animal osteoarthritis model, both the small molecule 6 and the 6-treated hASCs exhibited enhanced recovery of injured articular cartilage. This work provides new insight into MSC differentiation induced by small molecules and potential new therapeutic approaches for articular cartilage injury.
Catholic Kwandong University International St. Mary's Hospital, Seo-go, Incheon Metropolitan City 22711, Republic of Korea; Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 25601, Republic of Korea.
Eunhyun
E
Choi
Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seodaemun-gu, Seoul 03722, Republic of Korea.
Jiyun
J
Lee
Catholic Kwandong University International St. Mary's Hospital, Seo-go, Incheon Metropolitan City 22711, Republic of Korea; Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 25601, Republic of Korea.
Seahyoung
S
Lee
Catholic Kwandong University International St. Mary's Hospital, Seo-go, Incheon Metropolitan City 22711, Republic of Korea; Institute for Integrative Medicine, College of Medicine, Catholic Kwandong University, Gangneung, Gangwon-do 25601, Republic of Korea.
Byeong-Wook
BW
Song
Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seodaemun-gu, Seoul 03722, Republic of Korea.
Hyang-Hee
HH
Seo
Catholic Kwandong University International St. Mary's Hospital, Seo-go, Incheon Metropolitan City 22711, Republic of Korea; Institute for Integrative Medicine, College of Medicine, Catholic Kwandong University, Gangneung, Gangwon-do 25601, Republic of Korea.
Min-Ji
MJ
Cha
Catholic Kwandong University International St. Mary's Hospital, Seo-go, Incheon Metropolitan City 22711, Republic of Korea; Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 25601, Republic of Korea.
Soyeon
S
Lim
Department of Integrated OMICS for Biomedical Sciences (WCU Program), Yonsei University, Seodaemun-gu, Seoul 03722, Republic of Korea; Translational Research Center for Protein Function Control, Department of Biotechnology, Yonsei University, Seodaemun-gu, Seoul 03722, Republic of Korea.
Chulho
C
Lee
Department of Cardiovascular Surgery, Gangnam Severance Hospital, Yonsei University College of Medicine, Gangnam-gu, Seoul 06273, Republic of Korea.
Suk-Won
SW
Song
Department of Integrated OMICS for Biomedical Sciences (WCU Program), Yonsei University, Seodaemun-gu, Seoul 03722, Republic of Korea; Translational Research Center for Protein Function Control, Department of Biotechnology, Yonsei University, Seodaemun-gu, Seoul 03722, Republic of Korea. Electronic address: gyoonhee@yonsei.ac.kr.
Gyoonhee
G
Han
Catholic Kwandong University International St. Mary's Hospital, Seo-go, Incheon Metropolitan City 22711, Republic of Korea; Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 25601, Republic of Korea. Electronic address: kchwang@cku.ac.kr.
Ki-Chul
KC
Hwang
D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects*; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D012038:Regeneration; D054852:Small Molecule Libraries; D013449:Sulfonamides / Q000494:pharmacology*
D003470:Culture Media; D054852:Small Molecule Libraries; D013449:Sulfonamides
Chondrogenesis; Mesenchymal stem cells; Osteoarthritis; Regenerative medicine; Sulfonamide