Secondary Metabolites of the Endophytic Fungus Lachnum abnorme from Ardisia cornudentata.
Fractionation of an EtOAc-soluble fraction of the solid fermentate of an endophytic fungus, Lachnum abnorme Mont. BCRC 09F0006, derived from the endemic plant, Ardisia cornudentata Mez. (Myrsinaceae), resulted in the isolation of three new chromones, lachnochromonins D-F (1-3), one novel compound, lachabnormic acid (4), along with nine known compounds (5-13). Their structures were elucidated by spectroscopic analyses. Alternariol-3,9-dimethyl ether (6) was given the correct data as well as 2D spectral analyses for the first time. This is the first report of the isolation of one unprecedented compound 4 from Lachnum genus, while all known compounds were also found for the first time from Lachnum. The effects of some isolates (3, 4, 7-9, 10, and 13) on the inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-activated RAW 264.7 murine macrophages were also evaluated. Several compounds exhibited weak inhibitory activity on lipopolysaccharide (LPS)-stimulated NO production in RAW 264.7 macrophages.
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. hschang@kmu.edu.tw.|School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. hschang@kmu.edu.tw.
Hsun-Shuo
HS
Chang
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. chuhung.lin@gmail.com.
Chu-Hung
CH
Lin
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. shelfy25@hotmail.com.
Yi-Shuan
YS
Chen
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. wanghc@cc.kmu.edu.tw.
Hui-Chun
HC
Wang
Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan. chy50@firdi.org.tw.
Hing-Yuen
HY
Chan
Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan. sungyuan@gmail.com.
Sung-Yuan
SY
Hsieh
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. duncanwu762001@hotmail.com.
Ho-Cheng
HC
Wu
Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan. chengmingjen2001@yahoo.com.tw.
Ming-Jen
MJ
Cheng
Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan. gfy@firdi.org.tw.
Gwo-Fang
GF
Yuan
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. molly0627@hotmail.com.
Shan-Yu
SY
Lin
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. cccwei7569@gmail.com.
Yue-Jin
YJ
Lin
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. m635013@kmu.edu.tw.|School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. m635013@kmu.edu.tw.
Ih-Sheng
IS
Chen
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D031644:Ardisia / Q000382:microbiology; D001203:Ascomycota / Q000737:chemistry* / Q000302:isolation & purification; D002457:Cell Extracts / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002867:Chromones / Q000737:chemistry* / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D002457:Cell Extracts; D002867:Chromones; D006573:Heterocyclic Compounds, 1-Ring; C000616115:lachabnormic acid; D009569:Nitric Oxide
Ardisia cornudentata; Lachnum abnorme; anti-inflammatory activity; myrsinaceae; stem