Collagen analysis of the ascending aortic dilatation associated with bicuspid aortic valve disease compared with tricuspid aortic valve.
Dilatation of the ascending aorta is a common occurrence in patients with bicuspid aortic valve (BAV). The aim of the current study was to characterize collagen content in advanced glycation end products (AGEs) of dilated aortic tissue from two distinct areas, concave and convex aortic sites in patients with BAV and TAV. Collagen contents extracted from 100 mg tissue was isolated by enzymatic digestion using pepsin and the nondigested material was further digested using cyanogen bromide, insoluble collagen fraction (ICF) was extracted by hydrochloric acid hydrolysis. BAV tissue showed diminished fluorescence of the pepsin extracted fraction (PEF) compared with TAV tissue (12.4 ± 1.0% vs 32.9 ± 7.6%, p = 0.05). Patients with BAV had PEF of collagens significantly diminished in the dilated ascending aorta, especially in its convex portion, in course of aging and increment of dilated diameters. It is suggestible that BAV patients present more highly AGE-modified collagens in their ascending aorta.
a Department of heart surgery , Martin Luther University , Halle-Wittenberg , Germany and.
Alexander
A
Navarrete Santos
b Department of Cardiac and Thoracic Vascular Surgery , UKSH-Campus Luebeck , Luebeck , Germany.
Junfeng
J
Yan
a Department of heart surgery , Martin Luther University , Halle-Wittenberg , Germany and.
Peter
P
Lochmann
b Department of Cardiac and Thoracic Vascular Surgery , UKSH-Campus Luebeck , Luebeck , Germany.
Heike
H
Pfeil
b Department of Cardiac and Thoracic Vascular Surgery , UKSH-Campus Luebeck , Luebeck , Germany.
Michael
M
Petersen
a Department of heart surgery , Martin Luther University , Halle-Wittenberg , Germany and.
Andreas
A
Simm
a Department of heart surgery , Martin Luther University , Halle-Wittenberg , Germany and.
Hendrik
H
Treede
b Department of Cardiac and Thoracic Vascular Surgery , UKSH-Campus Luebeck , Luebeck , Germany.
Hans H
HH
Sievers
b Department of Cardiac and Thoracic Vascular Surgery , UKSH-Campus Luebeck , Luebeck , Germany.
Salah A
SA
Mohamed
D000368:Aged; D001011:Aorta / Q000473:pathology*; D001018:Aortic Diseases / Q000150:complications* / Q000601:surgery; D001021:Aortic Valve / Q000002:abnormalities* / Q000473:pathology / Q000601:surgery; D000082882:Bicuspid Aortic Valve Disease; D003094:Collagen / Q000378:metabolism*; D004108:Dilatation, Pathologic / Q000175:diagnosis* / Q000209:etiology / Q000378:metabolism; D005260:Female; D006349:Heart Valve Diseases / Q000473:pathology* / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged; D014261:Tricuspid Valve / Q000473:pathology* / Q000601:surgery
D003094:Collagen
Bicuspid aortic valve; advanced glycation end products; collagen; dilatation