[Regulation of airway stem cell proliferation in idiopathic pulmonary fibrosis].
OBJECTIVE
To investigate the effect of fibroblasts on regulating airway stem cell proliferation in idiopathic pulmonary fibrosis.


METHODS
Lung cell suspension was prepared from β-actin-GFP mice. Airway stem cells were obtained by fluorescence activated cell sorting and co-cultured with lung fibroblasts. The fibroblasts were treated with TGF-β inhibitor SB43142. The expression of growth factors FGF1/2 and the effect of FGF1/2 on stem cell proliferation were observed.


RESULTS
The cloning efficiency of airway stem cells, when co-cultured with normal lung fibroblast cells for 8 days, was (3.5±1.1)%, while the cloning efficiency was reduced to (0.04±0.04)% when co-cultured with lung fibroblasts from idiopathic pulmonary fibrosis patients. The difference between the 2 groups was statistically significant(P=0.002 5). TGF-β receptor inhibitor SB431542 increased lung fibroblast growth factors FGF1/2 expression.FGF1 mRNA expression was increased to the experimental group 0.005 5 from 0.000 2 in the control group.FGF2 mRNA expression of the amount raised to the experimental group 0.000 15 from 0.000 8 in the control group.FGF1/2 promoted the growth of airway stem cells. After FGF1/2 was co-cultured with normal lung fibroblast cells for 8 days, the cloning efficiency of airway stem cells was (0.3±0.1)%.


CONCLUSIONS
During the development of idiopathic pulmonary fibrosis, fibroblast secreted FGF1/2 regulate airway stem cell proliferation.
Tianjin Medical University, Tianjin Hospital, Tianjin 300070, China.
S X
SX
Yang
Q
Q
Wu
X
X
Sun
X
X
Li
K
K
Li
L
L
Xu
Y
Y
Li
Q Y
QY
Zhang
Y C
YC
Zhang
H Y
HY
Chen
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001549:Benzamides; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects; D004149:Dioxoles; D016220:Fibroblast Growth Factor 1 / Q000096:biosynthesis / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis / Q000494:pharmacology*; D005347:Fibroblasts; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D054990:Idiopathic Pulmonary Fibrosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008168:Lung / Q000378:metabolism / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases; D011658:Pulmonary Fibrosis; D000077294:Receptor, Transforming Growth Factor-beta Type II; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta
C459179:4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide; D000199:Actins; D001549:Benzamides; D004149:Dioxoles; D035683:MicroRNAs; D017468:Receptors, Fibroblast Growth Factor; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II