Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro.
BACKGROUND
Endometritis reduces fertility and is responsible for major economic losses in beef and dairy industries. The aim of this study was to evaluate an alternative therapy using platelet-rich plasma (PRP). PRP was tested in vivo, after bovine intrauterine administration, and in vitro on endometrial cells.


METHODS
Bovine endometrial cells were cultured until passage (P) 10 with 5 % or 10 % PRP. Effect of PRP on endometrial cell proliferation and on the expression of genes [prostaglandin-endoperoxide synthase 2 (COX2), tumor protein p53 (TP53), oestrogen receptors (ER-α and ER-β), progesterone receptor (PR) and c-Myc] involved in the regulation of oestrus cycle and fetal-maternal interaction were evaluated. Moreover, to evaluate the ability of PRP to counteract inflammation, 10 and 100 ng/ml of bacterial endotoxin lipopolysaccharide (LPS) were used to inflame endometrial cells in vitro for 1, 6, 12, 24 and 48 h. The expression of genes such as interleukin 1β (IL-1β), interleukin-8 (IL-8), inducible nitric oxide synthase (iNOS), prostaglandin-endoperoxide synthase 2 (COX2/PTGS2), and the release of PGE-2, IL-1β and IL-8 were evaluated.


RESULTS
In vivo treatment with PRP increased the detection of PR. In vitro, 5 % PRP at passage 5 increased proliferation rate and induced a significant increase in the expression of all studied genes. Furthermore, the results revealed that 10 ng/ml of LPS is the most effective dose to obtain an inflammatory response, and that PRP treatment significantly down regulated the expression of pro-inflammatory genes.


CONCLUSIONS
This study lays the foundations for the potential treatment of endometritis with PRP in vivo.
Department of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy.
Maria Giovanna
MG
Marini
Large Animal Hospital, Reproduction Unit, Università degli Studi di Milano, Via dell'Università 6, 26900, Lodi, Italy.
Claudia
C
Perrini
Large Animal Hospital, Reproduction Unit, Università degli Studi di Milano, Via dell'Università 6, 26900, Lodi, Italy.
Paola
P
Esposti
Department of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy.
Bruna
B
Corradetti
Department of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy.
Davide
D
Bizzaro
Large Animal Hospital, Anatomo-Pathology Unit, Università degli Studi di Milano, Lodi, Italy.
Pietro
P
Riccaboni
Large Animal Hospital, Anatomo-Pathology Unit, Università degli Studi di Milano, Lodi, Italy.
Eleonora
E
Fantinato
Private Practitioner, Milano, Italy.
Giuseppe
G
Urbani
Private Practitioner, Milano, Italy.
Giorgio
G
Gelati
Large Animal Hospital, Reproduction Unit, Università degli Studi di Milano, Via dell'Università 6, 26900, Lodi, Italy.
Fausto
F
Cremonesi
Large Animal Hospital, Reproduction Unit, Università degli Studi di Milano, Via dell'Università 6, 26900, Lodi, Italy. anna.langeconsiglio@unimi.it.
Anna
A
Lange-Consiglio
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D018920:Coculture Techniques; D004195:Disease Models, Animal; D004716:Endometritis / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism*; D053657:Platelet-Rich Plasma
D018836:Inflammation Mediators
Cattle; Endometrial cells; LPS; Platelet-rich plasma; gene expression