Evaluation of Inflammatory Response to Endodontic Sealers in a Bone Defect Animal Model.
OBJECTIVE
The aim of this study was to evaluate the inflammatory response to MTA Fillapex, AH Plus, and Pulp Canal Sealer Extensive Work Time (EWT), in a murine bone defect grafting model.


METHODS
Bilateral mandibular critical defects were produced in 45 Wistar rats with a trephine bur#2 and filled with the endodontic sealers. After 7, 14, and 28 days, the rats were euthanized and their jaws were histologically prepared.


RESULTS
For the 7-day group, no statistical significance was observed among all studied groups (p > 0.05), and high levels of inflammatory infiltrate were detected. After 14 and 28 days, Pulp Canal Sealer EWT showed statistically lower inflammatory response in comparison to other sealers (p < 0.05) except for the control group (no sealers).


CONCLUSIONS
Pulp Canal Sealer EWT presented the lowest levels of inflammatory response. The critical defect grafting model was an effective method to detect differences among differences on the biological response to endodontic sealers.


CONCLUSIONS
Knowing the biocompatibility of endodontics sealers that will be used in filling the root canal.
Professor, Department of Endodontics, Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil, Phone: +552199840270 e-mail: scelza@terra.com.br.
Miriam Z
MZ
Scelza
Department of Immunobiology, Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil.
Carlos Am
CA
Campos
Department of Endodontics, Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil.
Pantaleo
P
Scelza
Department of Dentistry, Fluminense Federal University Niteroi, Rio de Janeiro, Brazil.
Caroline Sr
CS
Adeodato
Department of Dentistry, Fluminense Federal University Niteroi, Rio de Janeiro, Brazil.
Igor B
IB
Barbosa
Department of Endodontics, Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil.
Fernando
F
de Noronha
Laboratory of Oral Pathology and Immunology and Molecular Biology, Sao Leopoldo Mandic, Campinas, Sao Paulo, Brazil.
Victor
V
Montalli
Laboratory of Oral Pathology and Immunology and Molecular Biology, Sao Leopoldo Mandic, Campinas, Sao Paulo, Brazil.
Marcelo
M
Napimoga
Laboratory of Oral Pathology and Immunology and Molecular Biology, Sao Leopoldo Mandic, Campinas, Sao Paulo, Brazil.
Vera C
VC
de Araújo
Department of Molecular and Cell Biology, Institute of Biology, Fluminense Federal University, Niteroi, Rio de Janeiro Brazil.
Gutemberg G
GG
Alves
D017607:Aluminum Compounds / Q000633:toxicity; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity; D017610:Calcium Compounds / Q000633:toxicity; D004195:Disease Models, Animal; D004338:Drug Combinations; D004853:Epoxy Resins / Q000633:toxicity; D008334:Mandible; D008422:Materials Testing; D010087:Oxides / Q000633:toxicity; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity
D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D004853:Epoxy Resins; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C534916:epoxy resin-based root canal sealer; C086631:mineral trioxide aggregate
Animal experimentation; Biocompatibility testing; Endodontics; Inflammation; Materials testing.