Evaluation of the relation between preapoptotic sperm rate in ejaculate and intrauterine insemination success rate in patients with unexplained infertility.
The parameters measured in the standard semen analysis may be insufficient for exact differentiation between fertile and infertile men. Therefore, we assume that the high rate of apoptotic sperm in ejaculate may play a role on the aetiology of unexplained infertility. Couples with unexplained infertility treated by ovulation induction and intrauterine insemination were consecutively enrolled (n = 94). To determine the proapoptotic sperm rate, the ejaculate from patients was stained with annexin V. Thirteen of the 94 couples (13.8%) conceived after intrauterine insemination. The annexin V-positive sperm rate was found to be 20.0% in the whole group. In women failing to conceive, the annexin V-positive sperm rate was 20.8% compared to 15.7% in patients who achieved pregnancy. Although there is a trend towards higher preapoptotic sperm rate in couples failing to get pregnant with insemination, the difference did not reach statistical significance.
a Department of Obstetrics and Gynaecology, Faculty of Medicine , Hacettepe University , Ankara , Turkey.
Gokhan
G
Boyraz
a Department of Obstetrics and Gynaecology, Faculty of Medicine , Hacettepe University , Ankara , Turkey.
Ilker
I
Selcuk
b Department of Histology and Embryology, Faculty of Medicine , Hacettepe University , Ankara , Turkey.
Lale K
LK
Sokmensuer
a Department of Obstetrics and Gynaecology, Faculty of Medicine , Hacettepe University , Ankara , Turkey.|c Division of Reproductive Medicine and Infertility, Faculty of Medicine , Hacettepe University , Ankara , Turkey.
Gurkan
G
Bozdag
d Department of Obstetrics and Gynaecology , Tygerberg Academic Hospital, Tygerberg , South Africa.
Roelof
R
Menkveld
a Department of Obstetrics and Gynaecology, Faculty of Medicine , Hacettepe University , Ankara , Turkey.|c Division of Reproductive Medicine and Infertility, Faculty of Medicine , Hacettepe University , Ankara , Turkey.
Serdar
S
Gunalp
D000328:Adult; D017304:Annexin A5; D017209:Apoptosis / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000209:etiology / Q000503:physiopathology*; D007315:Insemination, Artificial / Q000379:methods; D008297:Male; D010062:Ovulation Induction; D011247:Pregnancy; D011446:Prospective Studies; D055101:Semen Analysis / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods
D017304:Annexin A5
Annexin V; apoptosis; infertility; semen analysis