Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily.
Ophioviridae is a family of segmented, negative-sense, single-stranded RNA plant viruses. We showed that their cell-to-cell movement protein (MP) is an isolated member of the 30K MP superfamily with a unique structural organization. All 30K MPs share a core domain that contains a nearly-invariant signature aspartate. We examined its role in the MP of Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV). Alanine substitution of this aspartate prevented plasmodesmata accumulation of MP(MiLBVV), while MP(CPsV) was not affected. The capacity of ophiovirus MPs to increase the plasmodesmata size exclusion limit and non-cell autonomous protein feature was abolished in both mutants. To investigate the role of the signature aspartate in cell-to-cell movement, we constructed a new movement-deficient Tobacco mosaic virus vector used for trans-complementation assays. We showed that both ophiovirus MP mutants lack the cell-to-cell movement capacity, confirming that this signature aspartate is essential for viral cell-to-cell movement.
Instituto de Biotecnología y Biología Molecular, CCT-La Plata CONICET, Fac. Cs. Exactas, U.N.L.P., Calles 49 y 115, La Plata, Argentina.
María Belén
MB
Borniego
Department of Zoology, University of Oxford, Oxford OX1 3PS, UK; The Division of Structural Biology, Henry Wellcome Building, Roosevelt Drive, Oxford OX3 7BN, UK.
David
D
Karlin
Instituto de Biotecnología y Biología Molecular, CCT-La Plata CONICET, Fac. Cs. Exactas, U.N.L.P., Calles 49 y 115, La Plata, Argentina.
Eduardo José
EJ
Peña
Instituto de Biotecnología y Biología Molecular, CCT-La Plata CONICET, Fac. Cs. Exactas, U.N.L.P., Calles 49 y 115, La Plata, Argentina.
Gabriel
G
Robles Luna
Instituto de Biotecnología y Biología Molecular, CCT-La Plata CONICET, Fac. Cs. Exactas, U.N.L.P., Calles 49 y 115, La Plata, Argentina. Electronic address: garcia_m@biol.unlp.edu.ar.
María Laura
ML
García
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D019295:Computational Biology / Q000379:methods; D004252:DNA Mutational Analysis; D005810:Multigene Family; D009154:Mutation; D010641:Phenotype; D010935:Plant Diseases / Q000821:virology; D053830:Plant Viral Movement Proteins / Q000737:chemistry / Q000235:genetics*; D010942:Plant Viruses / Q000235:genetics*; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D012328:RNA Viruses / Q000235:genetics*
D053830:Plant Viral Movement Proteins
30K superfamily; Citrus psorosis virus; Mirafiori lettuce big-vein virus; Movement protein; Negative-strand RNA virus; Ophiovirus; TMV vector