Investigation of the binding of AuNPs-6-mercaptopurine and the sensitive detection of 6-mercaptopurine using resonance Rayleigh light scattering.
A highly sensitive method for the detection of 6-mercaptopurine (MP) by resonance Rayleigh light scattering (RLS) method was developed. Gold nanoparticles (AuNPs) were synthesized by a modified seed method and characterized using transmission electron microscopy (TEM). AuNPs were bound to MP via covalent bonding to form the MP-AuNPs complex, which increased the RLS intensity of MP at 347 nm (increased by 65.7%). Under optimum conditions, the magnitude of the enhanced RLS intensity for MP-AuNPs was proportional to MP concentration in the range 0.0681-1.702 μg mL-1. The linear regression equation was represented as follows: ΔIRLS= 9.31 + 82.42c (r = 0.9948). The limit of detection (LOD, 3σ) was 3.32 ng mL-1. The system was applied successfully to detect MP in pharmaceuticals. MP recoveries were 99.9-101.7% with a relative standard deviation (RSD) (n = 5) of 0.59-0.77% for three synthetic samples, and 97.5-110.0% with an RSD of 0.98-2.10% (n = 5) for tablet samples.
College of Chemistry, Changchun Normal University, Changchun, 130032, People's Republic of China.
Zhihong
Z
Li
College of Chemistry, Changchun Normal University, Changchun, 130032, People's Republic of China.
Shuyun
S
Bi
College of Chemistry, Changchun Normal University, Changchun, 130032, People's Republic of China.
Tianjiao
T
Wang
College of Chemistry, Changchun Normal University, Changchun, 130032, People's Republic of China.
Yu
Y
Wang
College of Chemistry, Changchun Normal University, Changchun, 130032, People's Republic of China.
Huifeng
H
Zhou
College of Chemistry, Changchun Normal University, Changchun, 130032, People's Republic of China.
Jun
J
Wu
D002138:Calibration; D000067493:Dynamic Light Scattering / Q000379:methods* / Q000592:standards; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D015122:Mercaptopurine / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009994:Osmolar Concentration; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013607:Tablets / Q000032:analysis
D013607:Tablets; D006046:Gold; D015122:Mercaptopurine
6-mercaptopurine; assembly; gold nanoparticles; resonance Rayleigh light scattering