New development and validation of 50 SSR markers in breadfruit (Artocarpus altilis, Moraceae) by next-generation sequencing.
OBJECTIVE
Using next-generation sequencing technology, new microsatellite loci were characterized in Artocarpus altilis (Moraceae) and two congeners to increase the number of available markers for genotyping breadfruit cultivars.


RESULTS
A total of 47,607 simple sequence repeat loci were obtained by sequencing a library of breadfruit genomic DNA with an Illumina MiSeq system. Among them, 50 single-locus markers were selected and assessed using 41 samples (39 A. altilis, one A. camansi, and one A. heterophyllus). All loci were polymorphic in A. altilis, 44 in A. camansi, and 21 in A. heterophyllus. The number of alleles per locus ranged from two to 19.


CONCLUSIONS
The new markers will be useful for assessing the identity and genetic diversity of breadfruit cultivars on a small geographical scale, gaining a better understanding of farmer management practices, and will help to optimize breadfruit genebank management.
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR AGAP, F-34398 Montpellier, France.
Fabien
F
De Bellis
Vanuatu Agricultural Research and Technical Centre (VARTC), P.O. Box 231, Santo, Vanuatu.
Roger
R
Malapa
Institut Agronomique néo-Calédonien (IAC), P.O. Box 73, 98890 Païta, New Caledonia.
Valérie
V
Kagy
Institut Agronomique néo-Calédonien (IAC), P.O. Box 73, 98890 Païta, New Caledonia.
Stéphane
S
Lebegin
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR AGAP, F-34398 Montpellier, France.
Claire
C
Billot
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR AGAP, F-34398 Montpellier, France.
Jean-Pierre
JP
Labouisse
Artocarpus altilis; Illumina; Moraceae; breadfruit; high-throughput sequencing; microsatellites