Aggregation-Induced Emission: Lighting up Cells, Revealing Life!
Understanding metabolism and dynamic biological events in cells, as well as physiological functions and pathological changes in organisms, is the major goal of biological investigations. It will improve our capability to diagnose and treat diseases, and will enhance personalized medicine. Fluorescence imaging is a powerful tool that plays an essential role in acquiring the comprehensive knowledge necessary to help reach this goal. Fluorescent molecules are crucial factors for obtaining high quality images. In contrast to conventional fluorogens with aggregation-caused quenching (ACQ) effect, molecules that show aggregation-induced emission (AIE) effect open up new avenues for fluorescence imaging. So far, a large variety of AIE probes have been developed and applied to bioimaging because of their outstanding characteristics, such as high fluorescence efficiency, excellent photostability and high signal-to-noise ratio (SNR). In this review, recent advances in AIE-based probes for biomedical imaging of intracellular microenvironments, natural macromolecules, subcellular organelles, intracellular processes, living tissues, and diagnosis and therapeutic evaluation of diseases in vivo are summarized. It is hoped that this review generates great research enthusiasm for AIE-based bioimaging, in order to promote the development of promising AIE probes and guide us to a better understanding of the biological essence of life.
CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Laboratory of Controllable Nanopharmaceuticals, National Center for Nanoscience and Technology of China, Beijing, 100190, P. R. China.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
Yi-Feng
YF
Wang
CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Laboratory of Controllable Nanopharmaceuticals, National Center for Nanoscience and Technology of China, Beijing, 100190, P. R. China.|School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, P. R. China.
Tingbin
T
Zhang
CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Laboratory of Controllable Nanopharmaceuticals, National Center for Nanoscience and Technology of China, Beijing, 100190, P. R. China.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
Xing-Jie
XJ
Liang
D000818:Animals; D006801:Humans; D061848:Optical Imaging / Q000379:methods*; D015203:Reproducibility of Results; D059629:Signal-To-Noise Ratio
aggregation-induced emission; cellular processes; in vivo imaging; subcellular organelles