Micro RNA-98 interferes with expression interleukin-10 in peripheral B cells of patients with lung cancer.
Interleukin (IL)-10-producing B cells (B10 cells) plays an important role in the tumor tolerance. High frequency of peripheral B10 cell was reported in patients with lung cancer recently. Micro RNA (miR) regulates some gene expression. This study test a hypothesis that miR-98 suppresses the expression of IL-10 in B cells of subjects with lung cancer. The results showed that the levels of miR-98 were significantly less in peripheral B cells of patients with lung cancer than that in healthy subjects. IL-10 mRNA levels in peripheral B cells were significantly higher in lung cancer patients as compared with healthy controls. A negative correlation was identified between miR-98 and IL-10 in peripheral B cells. Serum IL-13 was higher in lung cancer patients than that in healthy controls. The levels of IL-13 were also negatively correlated with IL-10 in B cells. Exposure B10 cells to IL-13 in the culture or over expression of miR-98 reduced the expression of IL-10 in B cells. Administration with miR-98-laden liposomes inhibited the lung cancer growth in a mouse model. In conclusion, up regulation of miR-98 inhibits the expression of IL-10 in B cells, which may contribute to inhibit the lung cancer tolerance in the body.
Department of Cardiothoracic Surgery, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Yun
Y
Li
Department of Anesthesiology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Jian
J
Rong
Department of Radiology, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Jie
J
Qin
Department of Cardiothoracic Surgery, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Jin-Yuan
JY
He
Department of Cardiothoracic Surgery, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Hui-Guo
HG
Chen
Department of Cardiothoracic Surgery, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Shao-Hong
SH
Huang
D000328:Adult; D000368:Aged; D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology* / Q000502:physiology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008081:Liposomes / Q000494:pharmacology; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D008297:Male; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics* / Q000494:pharmacology; D008875:Middle Aged; D023041:Xenograft Model Antitumor Assays
C508609:IL10 protein, human; D008081:Liposomes; C546084:MIRN98 microRNA, human; D035683:MicroRNAs; D016753:Interleukin-10