1,3-Bis(2-chloroethyl)-1-nitrosourea-loaded bovine serum albumin nanoparticles with dual magnetic resonance-fluorescence imaging for tracking of chemotherapeutic agents.
To date, knowing how to identify the location of chemotherapeutic agents in the human body after injection is still a challenge. Therefore, it is urgent to develop a drug delivery system with molecular imaging tracking ability to accurately understand the distribution, location, and concentration of a drug in living organisms. In this study, we developed bovine serum albumin (BSA)-based nanoparticles (NPs) with dual magnetic resonance (MR) and fluorescence imaging modalities (fluorescein isothiocyanate [FITC]-BSA-Gd/1,3-bis(2-chloroethyl)-1-nitrosourea [BCNU] NPs) to deliver BCNU for inhibition of brain tumor cells (MBR 261-2). These BSA-based NPs are water dispersible, stable, and biocompatible as confirmed by XTT cell viability assay. In vitro phantoms and in vivo MR and fluorescence imaging experiments show that the developed FITC-BSA-Gd/BCNU NPs enable dual MR and fluorescence imaging for monitoring cellular uptake and distribution in tumors. The T1 relaxivity (R1) of FITC-BSA-Gd/BCNU NPs was 3.25 mM(-1) s(-1), which was similar to that of the commercial T1 contrast agent (R1 =3.36 mM(-1) s(-1)). The results indicate that this multifunctional drug delivery system has potential bioimaging tracking of chemotherapeutic agents ability in vitro and in vivo for cancer therapy.
Department of Neurosurgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, Taoyuan.
Kuo-Chen
KC
Wei
Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung.
Feng-Wei
FW
Lin
Department of Neurosurgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, Taoyuan.
Chiung-Yin
CY
Huang
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, Republic of China.
Chen-Chi M
CC
Ma
Department of Neurosurgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, Taoyuan.
Ju-Yu
JY
Chen
Department of Neurosurgery, Chang Gung Memorial Hospital, School of Medicine, Chang Gung University, Taoyuan.
Li-Ying
LY
Feng
Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung.
Hung-Wei
HW
Yang
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002330:Carmustine / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003287:Contrast Media / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D016503:Drug Delivery Systems; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D005453:Fluorescence; D006801:Humans; D020128:Inhibitory Concentration 50; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008810:Mice, Inbred C57BL; D057054:Molecular Imaging; D053758:Nanoparticles / Q000008:administration & dosage*; D009369:Neoplasms / Q000188:drug therapy; D061848:Optical Imaging; D019047:Phantoms, Imaging; D012710:Serum Albumin, Bovine / Q000008:administration & dosage*; D017550:Spectroscopy, Fourier Transform Infrared
D000970:Antineoplastic Agents; D003287:Contrast Media; D003432:Cross-Linking Reagents; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate; D002330:Carmustine
BSA nanoparticles; MR imaging; cancer therapy; drug tracking; fluorescence imaging