Corydalis bungeana Turcz. attenuates LPS-induced inflammatory responses via the suppression of NF-κB signaling pathway in vitro and in vivo.
BACKGROUND
Corydalis bungeana Turcz. (C. bungeana) is one of traditionally used medicines in China and possesses various biological effects, such as anti-inflammatory, antibacterial activity and inhibition of the immune function of the host.


OBJECTIVE
we studied the anti-inflammatory effect and molecular mechanism involved of C. bungeana both in vitro and in vivo model system in which the inflammatory response was induced by LPS treatment.


METHODS
Anti-inflammatory activity of C. bungeana was investigated by LPS-induced RAW 264.7 macrophages and BALB/c mice. The production and expression of pro-inflammatory cytokines were evaluated by Griess reagent, ELISA kits and RT-qPCR, respectively. Phosphorylation status of IκBα and p65 was illustrated by western blot assay.


RESULTS
C. bungeana reduced the secretion of NO, TNF-α, IL-6 and IL-1β through inhibiting the protein expression of iNOS, TNF-α, IL-6 and IL-1β in vitro and in vivo. Western blot analysis suggested that C. bungeana supressed NF-κB activation via regulating the phosphorylation of IκBα and p65. Immunohistochemical assay also demostrated the histological inflammatory change in liver tissue.


CONCLUSIONS
The results indicate that C. bungeana supresses the activation of NF-κB signaling pathway through inhibiting phosphorylation of IκBα and p65, which results in good anti-inflammatory effect. In addition, C. bungeana attenuates inflammatory reaction by supressing the expression of various inflammatory cytokines both in vivo and in vitro.
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Province Academy of Chinese Medicine, Nanjing 210028, PR China; Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China.
Xiao-Ting
XT
Zhai
Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, PR China.
Jia-Quan
JQ
Chen
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, PR China.
Cui-Hua
CH
Jiang
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Province Academy of Chinese Medicine, Nanjing 210028, PR China.
Jie
J
Song
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Province Academy of Chinese Medicine, Nanjing 210028, PR China.
Dong-Yu
DY
Li
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Province Academy of Chinese Medicine, Nanjing 210028, PR China.
Hao
H
Zhang
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Province Academy of Chinese Medicine, Nanjing 210028, PR China.
Xiao-Bin
XB
Jia
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Province Academy of Chinese Medicine, Nanjing 210028, PR China.
Wei
W
Tan
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Department of Clinical Laboratory, Jiangsu Province Hospital on Integration Medicine, Nanjing 210028, PR China.
Shu-Xia
SX
Wang
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Province Academy of Chinese Medicine, Nanjing 210028, PR China.
Yi
Y
Yang
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, PR China; Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Province Academy of Chinese Medicine, Nanjing 210028, PR China. Electronic address: zfxcjq@126.com.
Fen-Xia
FX
Zhu
D000818:Animals; D002460:Cell Line; D031311:Corydalis / Q000737:chemistry*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*
D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II
Acetonitrile (PubChem CID: 6342); Acetylcorynoline (PubChem CID: 177015); Anti-inflammatory; C. bungeana; Corynoline (PubChem CID: 177014); DMSO (PubChem CID: 679); Dihydrosanguinarine (PubChem CID: 124069); EtOH (PubChem CID: 702); NF-κB; NaNO(2) (PubChem CID: 23668193); Pro-inflammatory cytokines; Protopine (PubChem CID: 4970); Triethylamine (PubChem CID: 8471)