Prognostic Investigations of Expression Level of Two Genes FasL and Ki-67 as Independent Prognostic Markers of Human Retinoblastoma.
In this study, expression of FasL and Ki-67 messenger RNA (FasL and Ki-67 mRNA) in human retinoblastoma (HRB) was examined by the immunohistochemistry method and quantitative real-time PCR. Positive expression of Ki-67 in tumor cells was detected in 16 of 30 patients (53.33%), and only 9 (30%) of the tissues from patients with retinoblastoma showed positive staining for FasL. Our results revealed that FasL expression was significantly higher in tumor tissue with invasion compared with the noninvasion form (p = 0.033). Ki-67 expression was markedly increased in tumor tissues with invasion compared with the noninvasion group (p = 0.04), but no significant correlation was found between FasL expression and differentiation (p > 0.05). In addition, Ki-67 expression was strongly linked to differentiation (p < 0.002). Expression of these FasL was correlated with shorter overall survival of patients, but its expression was not significantly associated with overall survival (p = 0.15). The impact of Ki-67 expression on survival in patients was also evaluated. Ki-67 expression level was not found to be significantly associated with shorter survival (Kaplan-Meier; p = 0.09). Univariate analysis revealed that massive choroidal invasion was correlated with poor prognosis. Taken together, the data suggest that massive choroidal invasion is also an important indicator of poor prognosis for HRB.
Department of Pediatrics, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Samaneh
S
Kouzegaran
Department of Ophthalmology, Alzahra Eye Hospital, Zahedan University of Medical Sciences, Zahedan, Iran.
Kourosh
K
Shahraki
Department of Ophthalmology, AJA University of Medical Sciences, Tehran, Iran.
Ali
A
Makateb
Department of Optometry, School of Rehabilitation, Zahedan University of Medical Sciences, Zahedan, Iran.
Farkhondeh
F
Shahri
Department of Radiology, Stanford University, School of Medicine, Stanford, CA, USA.
Negin
N
Hatami
Department of Molecular Biology, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Vahid
V
Behnod
Department of Anesthesia, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
Amir Saber
AS
Tanha
D014408:Biomarkers, Tumor; D002648:Child; D002675:Child, Preschool; D053222:Fas Ligand Protein / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007223:Infant; D053208:Kaplan-Meier Estimate; D019394:Ki-67 Antigen / Q000235:genetics* / Q000378:metabolism; D008297:Male; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D063868:Patient Outcome Assessment; D011379:Prognosis; D012175:Retinoblastoma / Q000235:genetics* / Q000401:mortality* / Q000473:pathology
D014408:Biomarkers, Tumor; D053222:Fas Ligand Protein; D019394:Ki-67 Antigen