Inhibition of mixed fungal and bacterial biofilms on silicone by carboxymethyl chitosan.
Mixed biofilms with fungi and bacteria are the leading cause for the failure of medical silicone devices, such as voice prostheses in laryngectomy. In this study, we determined the effect of carboxymethyl chitosan (CM-chitosan) on mixed biofilm formation of fungi and bacteria on silicone which is widely used for construction of medical devices. Mixed biofilm formations were inhibited 72.87% by CM-chitosan. Furthermore, CM-chitosan significantly decreased the metabolic activity of the biofilms using 2, 3-bis (2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5 carboxanilide (XTT) reduction assay. The examination using confocal laser scanning microscopy and scanning electron microscope confirmed that CM-chitosan inhibited the mixed biofilm and damaged the cells. Effects of CM-chitosan on different stages of biofilms were also evaluated. CM-chitosan inhibited the adhesion of fungi and bacteria with an efficiency of >90%. It prevented biofilm formation at efficiencies of 69.86%, 50.88% and 46.58% when CM-chitosan was added at 90min, 12h and 24h after biofilm initiation, respectively. Moreover, CM-chitosan inhibited Candida yeast-to-hyphal transition. CM-chitosan was not only able to inhibit the metabolic activity of biofilms, but also active upon the establishment and development of biofilm. Therefore, CM-chitosan may serve as a possible antibiofilm agent to limit biofilm formation on voice prostheses.
Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria. Electronic address: yulong.tan@meduniwien.ac.at.
Yulong
Y
Tan
Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria. Electronic address: matthias.leonhard@meduniwien.ac.at.
Matthias
M
Leonhard
Department of Cranio-Maxillofacial and Oral Surgery, Medical University of Vienna, Vienna, Austria. Electronic address: doris.moser@meduniwien.ac.at.
Doris
D
Moser
Food Biotechnology Laboratory, Department of Food Sciences and Technology, BOKU-University of Natural Resources and Life Sciences, 1190 Vienna, Austria. Electronic address: masu277@gmail.com.
Su
S
Ma
Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria. Electronic address: berit.schneider-stickler@meduniwien.ac.at.
Berit
B
Schneider-Stickler
D001419:Bacteria / Q000378:metabolism*; D018441:Biofilms; D048271:Chitosan / Q000031:analogs & derivatives* / Q000378:metabolism; D005658:Fungi / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D012828:Silicones / Q000378:metabolism*
D012828:Silicones; C514968:carboxymethyl-chitosan; D048271:Chitosan
Carboxymethyl chitosan; Mixed fungal-bacterial biofilm