Thirty autosomal insertion-deletion polymorphisms analyzed using the Investigator®DIPplex Kit in populations from Iraq, Lithuania, Slovenia, and Turkey.
Thirty autosomal insertion-deletion (InDel) polymorphisms were analyzed in four populations from Iraq, Lithuania, Slovenia, and Turkey using the commercial kit Investigator®DIPplex. Genotyping issues were encountered for five of the 30 InDels. They were most probably caused by polymorphisms located in the primer binding sites. Population and forensic parameters were calculated. No significant deviations from Hardy-Weinberg equilibrium or significant linkage disequilibrium were detected. The observed heterozygosities ranged from 33% to 61% depending on the marker and the population. The combined probability of exclusion for the 30 markers was 99.7% in all four populations and the matching probabilities were 1 in 3-4×1012individuals. The multidimensional scaling plot drawn from FSTdistances showed a good concordance between the relative position of the 15 populations included in the plot and their geographic locations.
Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Frederik V's Vej 11, DK-2100, Copenhagen, Denmark. Electronic address: carmen.tomas@sund.ku.dk.
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Tomas
Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Frederik V's Vej 11, DK-2100, Copenhagen, Denmark.
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Poulsen
National Forensic Laboratory, Police, Ministry of the Interior, Vodovodna 95, 1000 Ljubljana, Slovenia.
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Drobnič
State Forensic Medicine Service under the Ministry of Justice of the Republic of Lithuania, Vilnius, Lithuania.
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Ivanova
State Forensic Medicine Service under the Ministry of Justice of the Republic of Lithuania, Vilnius, Lithuania.
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Jankauskiene
State Forensic Medicine Service under the Ministry of Justice of the Republic of Lithuania, Vilnius, Lithuania.
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Bunokiene
Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Frederik V's Vej 11, DK-2100, Copenhagen, Denmark.
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Børsting
Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Frederik V's Vej 11, DK-2100, Copenhagen, Denmark.
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Morling
D053523:Amelogenin / Q000235:genetics; D016172:DNA Fingerprinting; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D054643:INDEL Mutation; D007493:Iraq; D008097:Lithuania; D060885:Multiplex Polymerase Chain Reaction / Q000295:instrumentation*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D017524:Slovenia; D014421:Turkey
D053523:Amelogenin
Forensic genetics; Investigator(®) DIPplex; Iraq; Lithuania; Slovenia; Turkey