Cytokine Release Patterns in Mixed Lymphocyte Culture (MLC) of T-Cells with Dendritic Cells (DC) Generated from AML Blasts Contribute to Predict anti-Leukaemic T-Cell Reactions and Patients' Response to Immunotherapy.
To enlighten interactions between autologous, allogeneic or T-cells from patients after stem cell transplantation with leukaemia-derived-dendritic-cells containing dendritic cells or blast containing mononuclear cells (n = 21, respectively), we determined cytokine-concentrations (interleukin 2, 4, 6, 10, tumor-necrosis-factor-α, interferon-γ) in supernatants of mixed-lymphocyte-culture and in serum (n = 16) of 20 patients with acute myeloid leukaemia and three patients with myelodysplastic syndromes by cytometric-bead-assay. We correlated our data with lytic capabilities of stimulated T-cells in a fluorolysis-assay and clinical data: Dendritic-cell-/mononuclear-cell-stimulation of T-cells resulted in increased cytokine-levels in culture-medium compared to serum. There were no significant differences between cytokine-patterns of cases with/without lytic T-cell-activity, response to immunotherapy (stem cell transplantation/donor-lymphocyte-infusion) or graft-versus-host-disease. However, some predictive cytokine-cut-off-values for antileukaemic T-cell-activity, patients' response to immunotherapy and graft-versus-host-disease could be defined. Cytokine-profiles alone, without functional assays, are no useful tool to predict antileukaemic T-cell-function, although they can indicate lytic T-cell-activity, patients' response to immunotherapy and graft-versus-host-disease.
a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.
Dorothea
D
Fischbacher
a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.
Marion
M
Merle
a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.
Anja
A
Liepert
a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.
Christine
C
Grabrucker
a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.
Tanja
T
Kroell
a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.
Andreas
A
Kremser
a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.
Julia
J
Dreyßig
a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.
Markus
M
Freudenreich
b Department for pediatric Haematology and Oncology , University Hospital of Düsseldorf , Düsseldorf , Germany.
Friedhelm
F
Schuster
b Department for pediatric Haematology and Oncology , University Hospital of Düsseldorf , Düsseldorf , Germany.
Arndt
A
Borkhardt
c Municipal Hospital Oldenburg , Oldenburg , Germany.
Doris
D
Kraemer
c Municipal Hospital Oldenburg , Oldenburg , Germany.
Claus-Henning
CH
Koehne
a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.|d Helmholtz Center, Munich, Clinical cooperative group Human Cell Transplantation (CCG-HCT) , Munich , Germany.
Hans-Jochem
HJ
Kolb
e Municipal Hospital Augsburg , Augsburg , Germany.
Christoph
C
Schmid
a Department for Haematopoietic Transplantations , University Hospital of Munich , Munich , Germany.|d Helmholtz Center, Munich, Clinical cooperative group Human Cell Transplantation (CCG-HCT) , Munich , Germany.
Helga Maria
HM
Schmetzer
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D016207:Cytokines / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D015470:Leukemia, Myeloid, Acute / Q000276:immunology / Q000628:therapy*; D008213:Lymphocyte Activation / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D033581:Stem Cell Transplantation / Q000379:methods; D013601:T-Lymphocytes / Q000276:immunology*; D055815:Young Adult
D016207:Cytokines
Dendritic cells; T-cells; acute myeloid leukaemia; cytokines; immunotherapy; stem cell transplantation