Complimentary action: C1q increases ganglion cell survival in an in vitro model of retinal degeneration.
Using a previously described retinal explant culture system as an acute injury model, we here explore the role of C1q, the initiator of the classical complement pathway, in neuronal cell survival and retinal homeostasis. Full-thickness adult rat retinal explants were divided into four groups, receiving the following supplementation: C1q (50nM), C1-inhibitor (C1-inh; Berinert; 500mg/l), C1q+C1-inh, and no supplementation (culture controls). Explants were kept for 12h or 2days after which they were examined morphologically and with a panel of immunohistochemical markers. C1q supplementation protects ganglion cells from degeneration within the explant in vitro system. This effect is correlated to an attenuated endogenous production of C1q, and a quiesced gliotic response.
Department of Clinical Sciences, Ophthalmology, Lund University, Lund, Sweden. Electronic address: linnea.taylor@med.lu.se.
Linnéa
L
Taylor
Department of Clinical Sciences, Ophthalmology, Lund University, Lund, Sweden.
Karin
K
Arnér
Department of Translational Medicine, Lund University, Malmö, Sweden.
Anna M
AM
Blom
Department of Clinical Sciences, Ophthalmology, Lund University, Lund, Sweden.
Fredrik
F
Ghosh
D000538:Aluminum Silicates / Q000494:pharmacology; D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D015922:Complement C1q / Q000037:antagonists & inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012162:Retinal Degeneration / Q000188:drug therapy / Q000473:pathology*; D012165:Retinal Ganglion Cells / Q000378:metabolism* / Q000473:pathology*; D012243:Rhodopsin / Q000378:metabolism; D013997:Time Factors
C100745:Aif1 protein, rat; D000538:Aluminum Silicates; D002135:Calcium-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D036341:Intercellular Signaling Peptides and Proteins; D008840:Microfilament Proteins; C436844:beringite; D015922:Complement C1q; D012243:Rhodopsin; D010751:Phosphopyruvate Hydratase; D005974:Glutamate-Ammonia Ligase
Adult retina; C1q; Ganglion cell; Neuroinflammation; Retinal degeneration; Retinal explant culture