Description of histopathological changes induced by the venom of the Persian Gulf Lionfish (Pterois russelli) in a mouse model of multiorgan toxicity.
Pterois russelli is a venomous fish belongs to Scorpaenidae family. Envenomation by the Persian Gulf lionfish is associated with local pain, marked inflammation and local heat. The present study was aimed to document the histopathological changes in liver, heart, lung, kidney and alterations in release of critical enzymes such as LDH, CK. AST, ALT and ALP induced by the administration of various doses of P. russelli venom in a mouse model. LD50 of venom was determined by intravenous injection in Balb/c mice. Histopathological alterations of lung, liver, heart and kidney following injection of one LD50, 1/2 and 1/3 LD50 doses of the venom were evaluated. Simultaneously, release of LDH, CK, AST, ALT and ALP were measured in serum following administration of 1/2 and1/3 LD50 doses of the venom too. LD50 was calculated as 10.5 mg/kg. The level of all enzymes were increased after 3 h and significantly raised after 24 h and rapidly reduced after 48 h. Histological studies showed that one LD50 and 1/2 LD50 doses of the venom induced significant histological alterations in the lungs, liver, heart and kidneys including congestion, hemorrhage, necrosis, apoptosis, edema, and infiltration of inflammatory cells. The results indicate that the venom of P. russelli has nephrotoxic, hepatotoxic, cardiotoxic and pneumotoxic effects in mouse model. Among four examined vital organs, the highest critical events were seen in liver. The findings are useful to give new insight in the fish's venom studies. Gathering the data resulted from this study together will be directed us toward a good aspect concerning the toxicity of potential therapeutic molecules in the venom of lionfish.
Department of Marine Biology, Faculty of Marine Sciences and Technologies, Science and Research Branch, Islamic Azad University, Tehran, Iran. Electronic address: memar150@yahoo.com.
Bahareh
B
Memar
Department of Marine Biology, Faculty of Marine Sciences and Technologies, Science and Research Branch, Islamic Azad University, Tehran, Iran. Electronic address: shahlajamili45@yahoo.com.
Shahla
S
Jamili
Biotechnology Research Center, Medical Biotechnology Dept., Venom and Biotherapeutics Molecules Lab., Pasteur Institute of Iran, Tehran, Iran. Electronic address: shahbazzadeh@yahoo.com.
Delavar
D
Shahbazzadeh
Biotechnology Research Center, Medical Biotechnology Dept., Venom and Biotherapeutics Molecules Lab., Pasteur Institute of Iran, Tehran, Iran. Electronic address: k_bagheri@pasteur.ac.ir.
Kamran
K
Pooshang Bagheri
D000818:Animals; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D006321:Heart / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008387:Marine Toxins / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D010473:Perciformes
D008387:Marine Toxins
Histopathology; Lionfish; Pterois russelli; Serum biochemical parameters; The Persian Gulf; Venom