No diurnal variation of classical and candidate biomarkers of Alzheimer's disease in CSF.
Cerebrospinal fluid (CSF) biomarkers have gained increasing importance in the diagnostic work-up of Alzheimer's disease (AD). The core CSF biomarkers related to AD pathology (Aβ42, t-tau and p-tau) are currently used in CSF diagnostics, while candidate markers of amyloid metabolism (Aβ38, Aβ40, sAPPα, sAPPβ), synaptic loss (neurogranin), neuroinflammation (YKL-40), neuronal damage (VILIP-1) and genetic risk (apolipoprotein E) are undergoing evaluation. Diurnal fluctuation in the concentration of CSF biomarkers has been reported and may represent a preanalytical confounding factor in the laboratory diagnosis of AD. The aim of the present study was to investigate the diurnal variability of classical and candidate CSF biomarkers in a cohort of neurosurgical patients carrying a CSF drainage.



Samples were collected from a cohort of 13 neurosurgical patients from either ventricular (n = 6) or lumbar (n = 7) CSF drainage at six time points during the day, 1-7 days following the neurosurgical intervention. Concentrations of the core biomarkers were determined by immunoassays.



Although absolute values largely varied among subjects, none of the biomarkers showed significant diurnal variation. Site of drainage (lumbar vs. ventricular) did not influence this result. The different immunoassays used for tau and Aβ markers provided similar results.



Time of day at CSF collection does not ultimately affect the concentration levels of classical and candidate AD biomarkers. Similar trends were found when using different immunoassays, thus corroborating the consistency of the data.
Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy at University of Gothenburg, House V3, SU / Mölndal hospital, Göteborgsvägen 31, SE-431 80, Mölndal, Sweden.|Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, SE-431 80, Mölndal, Sweden.
Claudia
C
Cicognola
Section of Neurology, Department of Medicine, Center for Memory Disturbances, University of Perugia, Sant' Andrea delle Fratte, 06132, Perugia, Italy.
Davide
D
Chiasserini
Section of Neurology, Department of Medicine, Center for Memory Disturbances, University of Perugia, Sant' Andrea delle Fratte, 06132, Perugia, Italy.
Paolo
P
Eusebi
Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy at University of Gothenburg, House V3, SU / Mölndal hospital, Göteborgsvägen 31, SE-431 80, Mölndal, Sweden.|Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, SE-431 80, Mölndal, Sweden.
Ulf
U
Andreasson
ADx NeuroSciences, Gent, Belgium.
Hugo
H
Vanderstichele
Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy at University of Gothenburg, House V3, SU / Mölndal hospital, Göteborgsvägen 31, SE-431 80, Mölndal, Sweden.|Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, SE-431 80, Mölndal, Sweden.|UCL Institute of Neurology, Queen Square, London, UK.
Henrik
H
Zetterberg
Section of Neurology, Department of Medicine, Center for Memory Disturbances, University of Perugia, Sant' Andrea delle Fratte, 06132, Perugia, Italy. lucilla.parnetti@unipg.it.
Lucilla
L
Parnetti
Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy at University of Gothenburg, House V3, SU / Mölndal hospital, Göteborgsvägen 31, SE-431 80, Mölndal, Sweden.|Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, SE-431 80, Mölndal, Sweden.
Kaj
K
Blennow
D000328:Adult; D000368:Aged; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D001057:Apolipoproteins E / Q000378:metabolism; D015415:Biomarkers / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000378:metabolism; D016875:tau Proteins / Q000134:cerebrospinal fluid*
D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E; D015415:Biomarkers; D010446:Peptide Fragments; D016875:tau Proteins