Evaluation of a Multiplex PCR Assay To Rapidly Detect Enterobacteriaceae with a Broad Range of β-Lactamases Directly from Perianal Swabs.
We developed and evaluated multiplexed molecular beacon probes in a real-time PCR assay to identify prominent extended-spectrum-β-lactamase, plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemase genes directly from perianal swab specimens within 6 h. We evaluated this assay on 158 perianal swabs collected from hematopoietic stem cell transplant recipients and found that this assay was highly sensitive and specific for detection of CTX-M-, pAmpC-, and KPC-producing Enterobacteriaceae compared to culture on chromogenic agar.
Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
Kalyan D
KD
Chavda
Transplantation-Oncology Infectious Diseases Program, Division of Infectious Diseases, Weill Cornell Medicine, New York, New York, USA.
Michael J
MJ
Satlin
Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
Liang
L
Chen
Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
Claudia
C
Manca
Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York, USA.
Stephen G
SG
Jenkins
Departments of Pediatrics and Microbiology & Immunology, Weill Cornell Medicine, New York, New York, USA.
Thomas J
TJ
Walsh
Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA kreiswba@njms.rutgers.edu.
Barry N
BN
Kreiswirth
D001003:Anal Canal / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D004755:Enterobacteriaceae / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D013048:Specimen Handling / Q000379:methods; D001618:beta-Lactamases / Q000235:genetics*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; C000602253:beta-lactamase CTX-M, E coli; C063912:carbapenemase