Adipocytes, like their progenitors, contribute to inflammation of adipose tissues through promotion of Th-17 cells and activation of monocytes, in obese subjects.
Recently, we have reported that adipose tissue-derived stem cells (ASC) harvested from obese donors induce a pro-inflammatory environment when co-cultured with peripheral blood mononuclear cells (MNC), with a polarization of T cells toward the Th17 cell lineage, increased secretion of IL-1β and IL-6 pro-inflammatory cytokines, and down-regulation of Th1 cytokines, such as IFNγ and TNFα. However, whether differentiated adipocytes, like the aforementioned ASC, are pro-inflammatory in obese subject AT remained to be investigated. Herein, we isolated ASC from AT of obese donors and differentiated them into adipocytes, for either 8 or 14 d. We analyzed their capacity to activate blood MNC after stimulation with phytohemagglutinin A (PHA), or not, in co-culture assays. Our results showed that co-cultures of MNC with adipocytes, like with ASC, increased IL-17A, IL-1β, and IL-6 pro-inflammatory cytokine secretion. Moreover, like ASC, adipocytes down-regulated TNFα secretion by Th1 cells. As adipocytes differentiated from ASC of lean donors also promoted IL-17A secretion by MNC, an experimental model of high-fat versus chow diet mice was used and supported that adipocytes from obese, but not lean AT, are able to mediate IL-17A secretion by PHA-activated MNCs. In conclusion, our results suggest that, as ASC, adipocytes in obese AT might contribute to the establishment of a low-grade chronic inflammation state.
CarMeN Laboratory, INSERM U1060, Lyon-1 University, INRA U1397, INSA-Lyon , Lyon, France.
Marwa
M
Chehimi
Clinical Research Department, Hospices Civils de Lyon , Lyon, France.
Maud
M
Robert
Clinical Research Department, Hospices Civils de Lyon , Lyon, France.
Michel El
ME
Bechwaty
CarMeN Laboratory, INSERM U1060, Lyon-1 University, INRA U1397, INSA-Lyon , Lyon, France.
Guillaume
G
Vial
CarMeN Laboratory, INSERM U1060, Lyon-1 University, INRA U1397, INSA-Lyon , Lyon, France.
Jennifer
J
Rieusset
CarMeN Laboratory, INSERM U1060, Lyon-1 University, INRA U1397, INSA-Lyon , Lyon, France.
Hubert
H
Vidal
CarMeN Laboratory, INSERM U1060, Lyon-1 University, INRA U1397, INSA-Lyon , Lyon, France.
Luciano
L
Pirola
CarMeN Laboratory, INSERM U1060, Lyon-1 University, INRA U1397, INSA-Lyon, Lyon, France; Clinical Research Department, Hospices Civils de Lyon, Center Hospitalier Lyon Sud, France.
Assia
A
Eljaafari
ASC; IL-17A; Th17; adipose-tissue derived stem cells; blood mononuclear cells; subcutaneous adipose tissue