Arsenic redox transformation by Pseudomonas sp. HN-2 isolated from arsenic-contaminated soil in Hunan, China.
A mesophilic, Gram-negative, arsenite[As(III)]-oxidizing and arsenate[As(V)]-reducing bacterial strain, Pseudomonas sp. HN-2, was isolated from an As-contaminated soil. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain was closely related to Pseudomonas stutzeri. Under aerobic conditions, this strain oxidized 92.0% (61.4μmol/L) of arsenite to arsenate within 3hr of incubation. Reduction of As(V) to As(III) occurred in anoxic conditions. Pseudomonas sp. HN-2 is among the first soil bacteria shown to be capable of both aerobic As(III) oxidation and anoxic As(V) reduction. The strain, as an efficient As(III) oxidizer and As(V) reducer in Pseudomonas, has the potential to impact arsenic mobility in both anoxic and aerobic environments, and has potential application in As remediation processes.
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, China; Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: zhangzhennan10@mails.ucas.ac.cn.
Zhennan
Z
Zhang
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, China; Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Naiyi
N
Yin
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, China; Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Xiaolin
X
Cai
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, China; Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Zhenzhou
Z
Wang
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, China; Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: cuiyanshan@ucas.ac.cn.
Yanshan
Y
Cui
D001151:Arsenic / Q000032:analysis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001711:Biotransformation; D002681:China; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
D012989:Soil Pollutants; D001151:Arsenic
Arsenate reduction; Arsenic; Arsenite oxidation; Detoxification; Pseudomonas