Preparation and antioxidant activities of oligosaccharides from Crassostrea gigas.
Oligosaccharides were prepared from Crassostrea gigas by hydrolysis of polysaccharide in C. gigas with peroxide oxygen (H2O2). The hydrolysates were cleared of protein, filtered, ultrafiltered and precipitated with absolute ethanol to give C. gigas oligosaccharides (CGOs). Factors affecting CGO yields, i.e., reaction time, temperature, and H2O2 concentration, were optimised as follows: 2.96h reaction time, 84.71°C reaction temperature, and 2.46% H2O2 concentration. Under these conditions, the maximum yield of CGOs reached 10.61%. The CGOs were then partially characterised by Fourier transform infrared spectroscopy, UV spectroscopy, monosaccharide composition, and antioxidant activities. Results indicate that CGOs possessed strong hydroxyl radical activity, 2,2-diphenyl-β-picrylhydrazyl-radical-scavenging activity and reducing capacity at a concentration of 100μg/mL.
Jiangsu Marine Resources Development Research Institute, Lianyungang, Jiangsu 222005, China; School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China; Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang 222005, China. Electronic address: wusj@hhit.edu.cn.
Shengjun
S
Wu
Jiangsu Marine Resources Development Research Institute, Lianyungang, Jiangsu 222005, China.
Xiaolian
X
Huang
D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D049896:Crassostrea; D016166:Free Radical Scavengers / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry
D000975:Antioxidants; D016166:Free Radical Scavengers; D009844:Oligosaccharides; D011134:Polysaccharides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide
Antioxidant activity; Crassostrea gigas; Oligosaccharides