Metabotropic glutamate receptors as targets of neuromodulatory influence of nitric oxide.
A possible effect of nitric oxide (NO) on metabotropic glutamate receptor (mGluR) function in the amino acid afferent synapse was investigated in the isolated labyrinth of the frog Rana temporaria. The modification of the amplitude of responses of metabotropic glutamate receptor agonist trans-ACPD was analyzed during bath applied NO donor S-nitroso-N-acetyl-DL-penicillamine SNAP (0.1-100 μM) or nitric oxide synthase inhibitor L-NAME. It was shown that NO donor SNAP (1 μM) inhibited mGluR induced responses, and the inhibitor of NO-synthase L-NAME (100 μM) increased the amplitude of trans-ACPD evoked answers. The results suggest that NO can depress mGluR function due to modulation of functions of the endoplasmic reticulum channels.
Laboratory of Physiology of Reception, Pavlov Institute of Physiology, RAS, St. Petersburg, Russia. ireneryzhova@mail.ru.
I V
IV
Ryzhova
Laboratory of Physiology of Reception, Pavlov Institute of Physiology, RAS, St. Petersburg, Russia.
A D
AD
Nozdrachev
Laboratory of Physiology of Reception, Pavlov Institute of Physiology, RAS, St. Petersburg, Russia.
T V
TV
Tobias
Laboratory of Physiology of Reception, Pavlov Institute of Physiology, RAS, St. Petersburg, Russia.
I V
IV
Orlov
Laboratory of Physiology of Reception, Pavlov Institute of Physiology, RAS, St. Petersburg, Russia.
V N
VN
Chikhman
Laboratory of Physiology of Reception, Pavlov Institute of Physiology, RAS, St. Petersburg, Russia.
S D
SD
Solnushkin
D029845:Amphibian Proteins / Q000378:metabolism*; D000818:Animals; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D011896:Rana temporaria; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism*; D026423:S-Nitroso-N-Acetylpenicillamine / Q000494:pharmacology
D029845:Amphibian Proteins; D018094:Receptors, Metabotropic Glutamate; D009569:Nitric Oxide; D026423:S-Nitroso-N-Acetylpenicillamine; D019331:NG-Nitroarginine Methyl Ester