Extensive characterization of peptides from Panax ginseng C. A. Meyer using mass spectrometric approach.
Panax ginseng is an important herb that has clear effects on the treatment of diverse diseases. Until now, the natural peptide constitution of this herb remains unclear. Here, we conduct an extensive characterization of Ginseng peptidome using MS-based data mining and sequencing. The screen on the charge states of precursor ions indicated that Ginseng is a peptide-rich herb in comparison of a number of commonly used herbs. The Ginseng peptides were then extracted and submitted to nano-LC-MS/MS analysis using different fragmentation modes, including CID, high-energy collisional dissociation, and electron transfer dissociation. Further database search and de novo sequencing allowed the identification of total 308 peptides, some of which might have important biological activities. This study illustrates the abundance and sequences of endogenous Ginseng peptides, thus providing the information of more candidates for the screening of active compounds for future biological research and drug discovery studies.
Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang, P. R. China.
Xueting
X
Ye
Division of Biological Technology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
Nan
N
Zhao
Food Science and Technology Programme, Department of Chemistry, National University of Singapore, Singapore.
Xi
X
Yu
Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang, P. R. China.
Xiaoli
X
Han
Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang, P. R. China. sypugaohy@163.com.
Huiyuan
H
Gao
Division of Biological Technology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
Xiaozhe
X
Zhang
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005894:Panax / Q000737:chemistry / Q000235:genetics*; D010455:Peptides / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry / Q000379:methods*
D010455:Peptides
Ginseng peptides; Nano-LC-MS/MS; PEAKS; Plant proteomics; Sequencing