Effect of Novel Compound LX519290, a Derivative of l-allo Threonine, on Antioxidant Potential in Vitro and in Vivo.
We investigated the antioxidative activity of LX519290, a derivative of l-allo threonine, in vitro and in vivo. To evaluate the antioxidative activity of LX519290, we performed several in vitro assays (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical-scavenging assays, a ferric reducing antioxidant power assay, cupric-reducing antioxidant capacity, and oxygen radical absorbance capacity assay) and evaluated inhibition against the generation of nitric oxide (NO) and reactive oxygen species (ROS) in murine macrophage (RAW264.7) cells. The results showed that LX519290 possessed very strong radical scavenging activity and reducing power, and inhibited NO and ROS generation in a dose-dependent manner without showing any cytotoxicity. LX519290 treatment also increased the total thiol content and glutathione S-transferases (GST) activities in RAW264.7 cells. Finally, we also determined whether LX519290 affects the mRNA levels of antioxidant enzymes in vitro and in vivo. The expression of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were markedly higher in the sample-treated group than in the oxidative stress group. LX519290 treatment also increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) with corresponding increases in the transcriptional and translational activities of haeme oxygenase-1 (HO-1). Collectively, the data demonstrated that LX519290 has potent antioxidative activity, decreases NO and ROS generation, increases total thiol content and GST activities in RAW264.7 cells, and increases the transcriptional and translational levels of antioxidant enzymes in vitro and in vivo.
Department of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, Korea. cg6890@hotmail.com.|Research and Development (R&D) Center, Huons Co., Ltd., Ansan 15588, Korea. cg6890@hotmail.com.
Kun
K
Chun
Department of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, Korea. mbalam@knu.ac.kr.
Md Badrul
MB
Alam
Department of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, Korea. vicpie@knu.ac.kr.
Hyeong-U
HU
Son
Department of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, Korea. sang@knu.ac.kr.
Sang-Han
SH
Lee
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism; D006834:Hydrazines / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013912:Threonine / Q000031:analogs & derivatives* / Q000494:pharmacology
D000975:Antioxidants; D006834:Hydrazines; C581088:LX519290; D051267:NF-E2-Related Factor 2; D013912:Threonine; D002374:Catalase; D005979:Glutathione Peroxidase; D006419:Heme Oxygenase (Decyclizing); D013482:Superoxide Dismutase
LX519290; antioxidative activity; in vitro; in vivo; ʟ-allo threonine