Microtubule Polymerization Functions in Hypersensitive Response and Accumulation of H2O2 in Wheat Induced by the Stripe Rust.
The plant cytoskeleton, including microtubules and microfilaments, is one of the important factors in determining the polarity of cell division and growth, as well as the interaction of plants with invading pathogens. In defense responses of wheat against the stripe rust (Puccinia striiformis f. sp. tritici) infection, hypersensitive response is the most crucial event to prevent the spread of pathogens. In order to reveal the effect of microtubules on the hypersensitive cell death and H2O2 accumulation in the interaction of wheat (Triticum aestivum) cv. Suwon 11 with an incompatible race, CYR23, wheat leaves were treated with microtubule inhibitor, oryzalin, before inoculation. The results showed that the frequency of infection sites with hypersensitive response occurrence was significantly reduced, and hypersensitive cell death in wheat leaves was suppressed compared to the control. In addition, the frequency and the incidence of infected cells with H2O2 accumulation were also reduced after the treatment with oryzalin. Those results indicated that microtubules are related to hypersensitive response and H2O2 accumulation in wheat induced by the stripe rust, and depolymerization of microtubules reduces the resistance of plants to pathogen infection in incompatible interaction, suggesting that microtubules play a potential role in the expression of resistance of wheat against the stripe rust fungus.
State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, China.
Juan
0000-0002-9905-5065
J
Wang
State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, China.
Yang
0000-0001-9614-580X
Y
Wang
State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, China.
Xinjie
X
Liu
State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, China.
Yuanliu
Y
Xu
State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, China.
Qing
0000-0003-0739-2281
Q
Ma
D001487:Basidiomycota / Q000472:pathogenicity*; D016923:Cell Death; D004136:Dinitrobenzenes; D054884:Host-Pathogen Interactions; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism*; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D013424:Sulfanilamides; D014908:Triticum / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology*
D004136:Dinitrobenzenes; D013424:Sulfanilamides; C012465:oryzalin; D006861:Hydrogen Peroxide