Dielectric Response of Cytoplasmic Water and Its Connection to the Vitality of Human Red Blood Cells: I. Glucose Concentration Influence.
The vitality of red blood cells depends on the process control of glucose homeostasis, including the membrane's ability to "switch off" d-glucose uptake at the physiologically specific concentration of 10-12 mM. We present a comprehensive study of human erythrocytes suspended in buffer solutions with varying concentrations of d-glucose at room temperature, using microwave dielectric spectroscopy (0.5 GHz-50 GHz) and cell deformability characterization (the Elongation ratio). By use of mixture formulas the contribution of the cytoplasm to the dielectric spectra was isolated. It reveals a strong dependence on the concentration of buffer d-glucose. Tellingly, the concentration 10-12 mM is revealed as a critical point in the behavior. The dielectric response of cytoplasm depends on dipole-matrix interactions between water structures and moieties, like ATP, produced during glycolysis. Subsequently, it is a marker of cellular health. One would hope that this mechanism could provide a new vista on noninvasive glucose monitoring.
Department of Applied Physics, The Rachel and Selim Benin School of Engineering and Computer Science, The Hebrew University of Jerusalem , Edmond J. Safra Campus, Jerusalem 91904, Israel.
Evgeniya
E
Levy
Department of Biochemistry & Molecular Biology, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem , Ein Kerem, Jerusalem 91120, Israel.
Gregory
G
Barshtein
Department of Biochemistry & Molecular Biology, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem , Ein Kerem, Jerusalem 91120, Israel.
Leonid
L
Livshits
Department of Applied Physics, The Rachel and Selim Benin School of Engineering and Computer Science, The Hebrew University of Jerusalem , Edmond J. Safra Campus, Jerusalem 91904, Israel.|Department of Physics, Ariel University , P.O.B. 3, Ariel 40700, Israel.
Paul Ben
PB
Ishai
Department of Applied Physics, The Rachel and Selim Benin School of Engineering and Computer Science, The Hebrew University of Jerusalem , Edmond J. Safra Campus, Jerusalem 91904, Israel.
Yuri
Y
Feldman
D003593:Cytoplasm / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D004912:Erythrocytes / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D006801:Humans; D014867:Water / Q000737:chemistry*
D014867:Water; D005947:Glucose