Evaluation of DNA barcode candidates for the discrimination of Artemisia L.
Because of the very similar morphologies and wide diversity of Artemisia L. varieties, they are difficult to identify, and there have been many arguments about the systematic classification Artemisia L., especially concerning the division of species. DNA barcode technology is used to rapidly identify species based on standard short DNA sequences. To evaluate seven candidate DNA barcodes (ITS, ITS2, psbA-trnH, rbcL, matK, rpoB, and rpoC1) regarding their ability to identify closely related species of the Artemisia genus in Xinjiang. The corresponding PCR amplification efficiency, detectable genetic divergence, identification efficiency and phylogenetic tree were assessed. We found that the internal transcribed spacer (ITS) region exhibited the highest interspecific divergence, which was significantly higher than the observed intraspecific variation and showed the highest identification efficiency, followed by ITS2, psbA-trnH and, finally, rpoB. matK, rbcL, and rpoC1 performed poorly in this evaluation. ITS, ITS2, and psbA-trnH were able to perfectly identify the tested species Artemisia annua, A. absinthium, A. rupestris, A. tonurnefortiana, A. austriaca, A. dracunculus, A. vulgaris, and A. macrocephala. Therefore, we propose the ITS, ITS2, and psbA-trnH regions as promising DNA barcodes for the closely related species of Artemisia L. in Xinjiang.
a Key Laboratory of Plant Resources and Chemistry in Arid Zone , Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi , Xinjiang , PR China.|b State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization , Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Urumqi , PR China.
Geyu
G
Liu
a Key Laboratory of Plant Resources and Chemistry in Arid Zone , Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi , Xinjiang , PR China.|b State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization , Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Urumqi , PR China.
Huixia
H
Ning
a Key Laboratory of Plant Resources and Chemistry in Arid Zone , Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi , Xinjiang , PR China.|b State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization , Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Urumqi , PR China.
Nurbolati
N
Ayidaerhan
a Key Laboratory of Plant Resources and Chemistry in Arid Zone , Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi , Xinjiang , PR China.|b State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization , Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Urumqi , PR China.
Haji Akber
HA
Aisa
D019306:Artemisia / Q000145:classification / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic; D018744:DNA, Plant; D014644:Genetic Variation; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D018744:DNA, Plant
Artemisia; DNA barcoding; identification; variation