Comparison of Human Neonatal and Adult Blood Leukocyte Subset Composition Phenotypes.
The human peripheral leukocyte subset composition depends on genotype variation and pre-natal and post-natal environmental influence diversity. We quantified this composition in adults and neonates, and compared the median values and dispersal ranges of various subsets in them. We confirmed higher frequencies of monocytes and regulatory T cells (Tregs), similar frequencies of neutrophils, and lower frequencies of CD8 T cells, NKT cells, B1 B cells and gamma-delta T cells in neonatal umbilical cord blood. Unlike previous reports, we found higher frequencies of eosinophils and B cells, higher CD4:CD8 ratios, lower frequencies of T cells and iNKT cells, and similar frequencies of CD4 T cells and NK cells in neonates. We characterized monocyte subsets and dendritic cell (DC) subsets in far greater detail than previously reported, using recently described surface markers and gating strategies and observed that neonates had lower frequencies of patrolling monocytes and lower myeloid dendritic cell (mDC):plasmacytoid DC (pDC) ratios. Our data contribute to South Asian reference values for these parameters. We found that dispersal ranges differ between different leukocyte subsets, suggesting differential determination of variation. Further, some subsets were more dispersed in adults than in neonates suggesting influences of postnatal sources of variation, while some show the opposite pattern suggesting influences of developmental process variation. Together, these data and analyses provide interesting biological possibilities for future exploration.
Pediatric Biology Center, Translational Health Science and Technology Institute, Faridabad, Haryana, India.|National Institute of Immunology, New Delhi, India.
Savit B
http://orcid.org/0000-0003-3486-8951
SB
Prabhu
Pediatric Biology Center, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
Deepak K
DK
Rathore
Pediatric Biology Center, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
Deepa
D
Nair
Pediatric Biology Center, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
Anita
A
Chaudhary
Pediatric Biology Center, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
Saimah
S
Raza
National Institute of Immunology, New Delhi, India.
Parna
P
Kanodia
Pediatric Biology Center, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
Shailaja
S
Sopory
National Institute of Immunology, New Delhi, India.
Anna
A
George
Pediatric Biology Center, Translational Health Science and Technology Institute, Faridabad, Haryana, India.|National Institute of Immunology, New Delhi, India.
Satyajit
S
Rath
Pediatric Biology Center, Translational Health Science and Technology Institute, Faridabad, Haryana, India.|National Institute of Immunology, New Delhi, India.
Vineeta
V
Bal
Department of Obstetrics & Gynecology, Maulana Azad Medical College, New Delhi, India.
Reva
R
Tripathi
Department of Neonatology, Maulana Azad Medical College, New Delhi, India.
Siddharth
S
Ramji
Department of Obstetrics & Gynecology, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India.
Aruna
A
Batra
Department of Pediatrics, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India.
Kailash C
KC
Aggarwal
Department of Pediatrics, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India.
Harish K
HK
Chellani
Department of Pediatrics, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India.
Sugandha
S
Arya
Department of Obstetrics and Gynecology, Gurgaon Civil Hospital, Gurgaon, India.
Nidhi
N
Agarwal
Department of Pediatrics, Gurgaon Civil Hospital, Gurgaon, India.
Umesh
U
Mehta
Pediatric Biology Center, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
Uma Chandra Mouli
UC
Natchu
Pediatric Biology Center, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
Nitya
N
Wadhwa
Pediatric Biology Center, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
Shinjini
S
Bhatnagar
D000293:Adolescent; D000328:Adult; D000375:Aging / Q000276:immunology*; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets; D055815:Young Adult