Turnera subulata Anti-Inflammatory Properties in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
In South America, particularly in the Northeastern regions of Brazil, Turnera subulata leaf extract is used as an alternative traditional medicine approach for several types of chronic diseases, such as diabetes, hypertension, chronic pain, and general inflammation. Despite its widespread use, little is known about the medicinal properties of the plants of this genus. In this study, we evaluate the antioxidant and anti-inflammatory of T. subulata leaf extract in an in vitro model of inflammation, using lipopolysaccharide-stimulated RAW-264.7 macrophage cell line. We observed that cotreatment with T. subulata leaf extract was able to reduce the oxidative stress in cells due to inflammatory response. More importantly, we observed that the leaf extract was able to directly modulate inflammatory response by altering activity of members of the mitogen-activated protein kinase pathways. Our results demonstrate for the first time that T. subulata have antioxidant and anti-inflammatory properties, which warrant further investigation of the medicinal potential of this species.
1 Institute of Tropical Medicine of Rio Grande do Norte, Federal University of Rio Grande do Norte , Natal, RN, Brazil .
Natália Cabral
NC
Souza
1 Institute of Tropical Medicine of Rio Grande do Norte, Federal University of Rio Grande do Norte , Natal, RN, Brazil .
Juliana Medeiros
JM
de Oliveira
2 Department of Biochemistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil .
Maurílio da Silva
MDS
Morrone
3 Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, Michigan, USA .
Ricardo D'Oliveira
RD
Albanus
4 Department of Morphology, Federal University of Rio Grande do Norte, Natal, RN, Brazil .
Maria do Socorro Medeiros
MDSM
Amarante
4 Department of Morphology, Federal University of Rio Grande do Norte, Natal, RN, Brazil .
Christina da Silva
CDS
Camillo
4 Department of Morphology, Federal University of Rio Grande do Norte, Natal, RN, Brazil .
Silvana Maria Zucolotto
SMZ
Langassner
2 Department of Biochemistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil .
Daniel Pens
DP
Gelain
2 Department of Biochemistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil .
José Cláudio Fonseca
JCF
Moreira
1 Institute of Tropical Medicine of Rio Grande do Norte, Federal University of Rio Grande do Norte , Natal, RN, Brazil .
Rodrigo Juliani Siqueira
RJS
Dalmolin
1 Institute of Tropical Medicine of Rio Grande do Norte, Federal University of Rio Grande do Norte , Natal, RN, Brazil .|5 Department of Food Engineering, Federal University of Campina Grande, Campina Grande, PB, Brazil .
Matheus Augusto
MA
de Bittencourt Pasquali