Study of the antioxidant activity of Thymus sibthorpii Bentham (Lamiaceae).
From the aerial parts of Thymus sibthorpii Bentham (Lamiaceae), five flavonoids apigenin (1), 7-methoxy-apigenin (2), naringenin (3), eriodictyol (4) and eriodictyol-7-glucoside (5), have been isolated together with caffeic acid methyl ester (6), rosmarinic acid (7) and rosmarinic acid methyl ester (8). The structures of the isolated compounds were established by spectroscopic methods. The extracts and the isolated compounds were tested for their free radical scavenging activity using the following in vitro assays: (i) interaction with the free stable radical of DPPH (1,1-diphenyl-2-picrylhydrazyl), (ii) inhibition of linoleic acid lipid peroxidation induced by the dihydrochloric acid of 2,2-azobis-2-amidinepropane (AAPH) and (iii) the scavenging activity of enzymatically produced superoxide anion. Their inhibitory activity toward soybean lipoxygenase was evaluated in vitro, using linoleic acid as a substrate. The antioxidant results of the extracts are discussed in terms of their constitution in phenolic compounds, which were determined following the Folin-Ciocalteu method.
a Laboratory of Hygiene and Environmental Protection , Department of Medicine, Democritus University of Thrace , Alexandroupolis , Greece.
Christos
C
Kontogiorgis
b Laboratory of Pharmacognosy , Department of Pharmacognocy and Pharmacology, School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki , Thessaloniki , Greece.|c Department of Nutrition and Dietetics , Faculty of Food Technology and Nutrition, Alexandrion Technological Educational Institution , Sindos , Thessaloniki , Greece , and.
Maria
M
Ntella
b Laboratory of Pharmacognosy , Department of Pharmacognocy and Pharmacology, School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki , Thessaloniki , Greece.|c Department of Nutrition and Dietetics , Faculty of Food Technology and Nutrition, Alexandrion Technological Educational Institution , Sindos , Thessaloniki , Greece , and.
Lina
L
Mpompou
b Laboratory of Pharmacognosy , Department of Pharmacognocy and Pharmacology, School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki , Thessaloniki , Greece.|c Department of Nutrition and Dietetics , Faculty of Food Technology and Nutrition, Alexandrion Technological Educational Institution , Sindos , Thessaloniki , Greece , and.
Fotini
F
Karallaki
c Department of Nutrition and Dietetics , Faculty of Food Technology and Nutrition, Alexandrion Technological Educational Institution , Sindos , Thessaloniki , Greece , and.
Papadopoulos
P
Athanasios
d Department of Pharmaceutical Chemistry , School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki , Thessaloniki , Greece.
Dimitra
D
Hadjipavlou-Litina
b Laboratory of Pharmacognosy , Department of Pharmacognocy and Pharmacology, School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki , Thessaloniki , Greece.
Diamanto
D
Lazari
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019686:Lamiaceae / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D008084:Lipoxygenase / Q000378:metabolism; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry; D013025:Glycine max / Q000201:enzymology; D013329:Structure-Activity Relationship
D000975:Antioxidants; D005419:Flavonoids; D008084:Lipoxygenase
Antioxidant activity; Lamiaceae; Thymus sibthorpii; flavonoids; lipoxygenase