Nanoscopic Cellular Imaging: Confinement Broadens Understanding.
In recent years, single-molecule fluorescence imaging has been reconciling a fundamental mismatch between optical microscopy and subcellular biophysics. However, the next step in nanoscale imaging in living cells can be accessed only by optical excitation confinement geometries. Here, we review three methods of confinement that can enable nanoscale imaging in living cells: excitation confinement by laser illumination with beam shaping; physical confinement by micron-scale geometries in bacterial cells; and nanoscale confinement by nanophotonics.
Department of Chemistry, University of Michigan , Ann Arbor, Michigan 48109, United States.
Stephen A
SA
Lee
Department of Chemistry, Cambridge University , Cambridge CB2 1EW, United Kingdom.
Aleks
A
Ponjavic
Department of Chemistry, University of Michigan , Ann Arbor, Michigan 48109, United States.
Chanrith
C
Siv
Department of Chemistry, Cambridge University , Cambridge CB2 1EW, United Kingdom.
Steven F
SF
Lee
Department of Chemistry, University of Michigan , Ann Arbor, Michigan 48109, United States.
Julie S
JS
Biteen
D001703:Biophysics; D008027:Light; D008856:Microscopy, Fluorescence; D036103:Nanotechnology
bacterial biophysics; light-sheet microscopy; nanophotonics; optical sectioning; plasmonic enhancement; single-molecule imaging; super-resolution microscopy