Fbxw7β, E3 ubiquitin ligase, negative regulation of primary myoblast differentiation, proliferation and migration.
Satellite cells attached to skeletal muscle fibers play a crucial role in skeletal muscle regeneration. During regeneration, the satellite cells proliferate, migrate to the damaged region, and fuse to each other. Although it is important to determine the cellular mechanisms controlling myoblast behavior, their regulators are not well understood. In this study, we evaluated the roles of Fbxw7 in primary myoblasts and determined its potential as a therapeutic target for muscle disease. We originally found that Fbxw7β, one of the E3 ubiquitin ligase Fbxw7 subtypes, negatively regulates differentiation, proliferation and migration of myoblasts and satellite cells on muscle fiber. However, these phenomena were not observed in myoblasts expressing a dominant-negative, F-box deleted Fbxw7β, mutant. Our results suggest that myoblast differentiation potential and muscle regeneration can be regulated by Fbxw7β.
Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Science, Seoul, South Korea.|Department of Biotechnology, Korea University, Seoul, South Korea.
Kyungshin
K
Shin
Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Science, Seoul, South Korea.
Sang-Gu
SG
Hwang
Department of Otorhinolaryngology-Head and Neck Surgery, Korea Cancer Center Hospital, Seoul, South Korea.
Ik Joon
IJ
Choi
College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.
Young-Gyu
YG
Ko
Department of Surgery, Hanyang University College of Medicine, Seoul, South Korea.
Jaemin
J
Jeong
Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Science, Seoul, South Korea.
Heechung
H
Kwon
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D002478:Cells, Cultured; D044783:F-Box Proteins / Q000502:physiology*; D000073979:F-Box-WD Repeat-Containing Protein 7; D008297:Male; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000502:physiology; D032448:Myoblasts, Skeletal / Q000166:cytology*; D012038:Regeneration / Q000235:genetics; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology*; D044767:Ubiquitin-Protein Ligases / Q000502:physiology*
D044783:F-Box Proteins; D000073979:F-Box-WD Repeat-Containing Protein 7; C513272:Fbxw7 protein, mouse; D044767:Ubiquitin-Protein Ligases
Fbxw7β; differentiation; migration; myoblast; skeletal muscle