Biosynthesis of Cr(III) nanoparticles from electroplating wastewater using chromium-resistant Bacillus subtilis and its cytotoxicity and antibacterial activity.
The aim of this study was to synthesize and characterize Cr(III) nanoparticles using wastewater from electroplating industries and chromium-resistant Bacillus subtilis. Formation of Cr(III) nanoparticles was confirmed by UV-visible (UV-Vis) spectroscopy at 300 nm. The size of the nanoparticles varied from 4 to 50 nm and energy dispersive spectroscopy profile shows strong Cr peak approximately at 4.45 and 5.2 keV. The nanoparticles inhibited the growth of pathogenic bacteria Staphylococcus aureus and Escherichia coli. The cytotoxic effect of the synthesized Cr(III) nanoparticle was studied using HEK 293 cells, and the cell viability was found to decrease with increasing concentration of Cr(III) nanoparticles.
a Department of Environmental Science, PSG College of Arts and Science , Coimbatore , Tamil Nadu , India.
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Kanakalakshmi
b Department of Chemistry , Sri Sarada College for Women , Salem , Tamil Nadu , India.
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Janaki
a Department of Environmental Science, PSG College of Arts and Science , Coimbatore , Tamil Nadu , India.
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Shanthi
c Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences , Chonbuk National University , Iksan , South Korea.
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Kamala-Kannan
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity; D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism*; D002857:Chromium / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology / Q000633:toxicity; D024881:Drug Resistance, Bacterial; D004595:Electroplating; D004926:Escherichia coli / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D036103:Nanotechnology; D013211:Staphylococcus aureus / Q000187:drug effects; D062065:Wastewater / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D002857:Chromium; D062065:Wastewater
Bacillus subtilis; biosynthesis; chromium; electroplating wastewater; nanoparticles