Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens.
Due to the increasing need of rapid tests for application in low resource settings, WHO summarized their ideal features under the acronym ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, Delivered to those who need it). In this work, two different platforms for the rapid and simultaneous testing of the foodborne pathogens E. coli O157:H7 and Salmonella enterica, in detail a nucleic acid lateral flow and an electrochemical magneto-genosensor are presented and compared in terms of their analytical performance. The DNA of the bacteria was amplified by polymerase chain reaction using a quadruple-tagging set of primers specific for E. coli eaeA (151bp) and Salmonella enterica yfiR (375bp) genes. During the amplification, the amplicons were labelled at the same time with biotin/digoxigenin or biotin/fluorescein tags, respectively. The nucleic acid lateral flow assay was based on the use of streptavidin gold nanoparticles for the labelling of the tagged amplicon from E. coli and Salmonella. The visual readout was achieved when the gold-modified amplicons were captured by the specific antibodies. The features of this approach are discussed and compared with an electrochemical magneto-genosensor. Although nucleic acid lateral flow showed higher limit of detection, this strategy was able to clearly distinguish positive and negative samples of both bacteria being considered as a rapid and promising detection tool for bacteria screening.
Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Spain.
A
A
Ben Aissa
Grup de Química Orgànica i Organometàl·lica, Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Spain.
J J
JJ
Jara
Grup de Química Orgànica i Organometàl·lica, Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Spain.
R M
RM
Sebastián
Grup de Química Orgànica i Organometàl·lica, Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Spain.
A
A
Vallribera
Grup de Microbiologia Molecular, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
S
S
Campoy
Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, Bellaterra, Spain. Electronic address: Isabel.Pividori@uab.cat.
M I
MI
Pividori
D015374:Biosensing Techniques / Q000295:instrumentation; D004269:DNA, Bacterial / Q000032:analysis*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005516:Food Microbiology; D006801:Humans; D057230:Limit of Detection; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D019779:Salmonella enterica / Q000302:isolation & purification*
D004269:DNA, Bacterial
Electrochemical magneto-genosensing; Foodborne bacteria; Magnetic particles; Nucleic acid lateral flow; Simultaneous detection