Coordinated autoinhibition of F-BAR domain membrane binding and WASp activation by Nervous Wreck.
Membrane remodeling by Fes/Cip4 homology-Bin/Amphiphysin/Rvs167 (F-BAR) proteins is regulated by autoinhibitory interactions between their SRC homology 3 (SH3) and F-BAR domains. The structural basis of autoregulation, and whether it affects interactions of SH3 domains with other cellular ligands, remain unclear. Here we used single-particle electron microscopy to determine the structure of the F-BAR protein Nervous Wreck (Nwk) in both soluble and membrane-bound states. On membrane binding, Nwk SH3 domains do not completely dissociate from the F-BAR dimer, but instead shift from its concave surface to positions on either side of the dimer. Unexpectedly, along with controlling membrane binding, these autoregulatory interactions inhibit the ability of Nwk-SH3a to activate Wiskott-Aldrich syndrome protein (WASp)/actin related protein (Arp) 2/3-dependent actin filament assembly. In Drosophila neurons, Nwk autoregulation restricts SH3a domain-dependent synaptopod formation, synaptic growth, and actin organization. Our results define structural rearrangements in Nwk that control F-BAR-membrane interactions as well as SH3 domain activities, and suggest that these two functions are tightly coordinated in vitro and in vivo.
Department of Bioengineering, Faculty of Biology, M.V. Lomonosov Moscow State University, 119234 Moscow, Russia;
Tatiana B
TB
Stanishneva-Konovalova
Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453.
Charlotte F
CF
Kelley
Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453.
Tania L
TL
Eskin
Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453.
Emily M
EM
Messelaar
Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453.
Steven A
SA
Wasserman
Department of Bioengineering, Faculty of Biology, M.V. Lomonosov Moscow State University, 119234 Moscow, Russia; sokolova184@gmail.com arodal@brandeis.edu.
Olga S
OS
Sokolova
Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453 sokolova184@gmail.com arodal@brandeis.edu.
Avital A
0000-0002-2051-8304
AA
Rodal
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D004330:Drosophila; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D011485:Protein Binding; D051317:Wiskott-Aldrich Syndrome Protein, Neuronal / Q000737:chemistry* / Q000235:genetics; D018909:src Homology Domains / Q000235:genetics
D029721:Drosophila Proteins; D008024:Ligands; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D051317:Wiskott-Aldrich Syndrome Protein, Neuronal; C483010:nwk protein, Drosophila
Drosophila; F-BAR; Nwk; WASp; actin