Radiological and Stereological Evaluation of the Effect of Rifampin on Bone Healing in Critical-Size Defects.
OBJECTIVE
This study aimed to investigate the effect of rifampin with autogenous bone on bone regeneration in critical-size defects in the calvaria of rats.


METHODS
In total, 40 rats were divided into 4 groups and a 5-mm diameter of calvarial defect was made in each rat's calvarium. Control group (C), bone defects were irrigated with sterile saline; rifampin group (R), bone defects were irrigated with rifampin. In the autogenous graft group (Ag), the autogenous graft was contaminated with saliva, and the defects were filled with an autogenous graft. In the autogenous graft + rifampin group (Ag+R), the autogenous graft was contaminated with saliva and was decontaminated with rifampin, and the defects were filled with the autogenous graft. The animals were killed at 4weeks. Bone formation was assessed by micro-computed tomography scanning and stereological analyses.


RESULTS
The mean new bone volume was the greatest in the Ag/rifampin group (1.73 ± 0.17), followed by the Ag group (1.50 ± 0.05) (statistically significant difference at P < 0.05). The new bone volume was the lowest in the control group (1.05 ± 0.09); however, no difference was observed compared with the rifampin group (1.08 ± 0.07) (P > 0.05).


CONCLUSIONS
This study, despite its limitations, showed that rifampin with autogenous bone increased bone regeneration in rats with critical-size defects.
*Department of Oral and Maxillofacial Surgery †Department of Periodontology, Faculty of Dentistry, Bülent Ecevit University, Zonguldak ‡Department of Histology and Embryology, Faculty of Medicine, Ondokuz Mayis University, Samsun §Department of Anatomy, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Mustafa Cenk
MC
Durmuşlar
Umut
U
Balli
Akif
A
Türer
Mehmet E
ME
Önger
Hakan H
HH
Çelik
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D017102:Fracture Healing / Q000187:drug effects*; D051381:Rats; D012293:Rifampin / Q000494:pharmacology / Q000627:therapeutic use; D012886:Skull / Q000000981:diagnostic imaging / Q000293:injuries; D055114:X-Ray Microtomography
D012293:Rifampin