Analysis of morphological and molecular composition changes in allergenic Artemisia vulgaris L. pollen under traffic pollution using SEM and FTIR spectroscopy.
Nowadays, pollen allergy becomes an increasing problem for human population. Common mugwort (Artemisia vulgaris L.) is one of the major allergenic plants in Europe. In this study, the influence of air pollution caused by traffic on the structure and chemical composition of common mugwort pollen was investigated. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and curve-fitting analysis of amide I profile was applied to assess the morphological and structural changes of mugwort pollen grains collected from sites with different vehicle pollution levels. Microscopic observations support the conclusion, that the higher the car traffic, the smaller the pollen grains. The obtained results clearly show that air pollution had an impact on different maximum absorbance values of individual functional groups composing the chemical structure of pollen. Moreover, air pollution induced structural changes in macromolecules of mugwort pollen. In pollen collected from the unpolluted site, the content of sporopollenin (850 cm-1) was the highest, whereas polysaccharide concentration (1032 cm-1) was the lowest. Significant differences were observed in lipids. Pollen collected from the site with heavy traffic had the lowest content of lipids at 1709, 2071, and 2930 cm-1. The largest differences were observed in the spectra regions corresponding to proteins. In pollen collected from unpolluted site, the highest level of β-sheet (1600 cm-1) and α-helix (1650 cm-1) was detected. The structural changes in proteins, observed in the second derivative of the FTIR spectrum and in the curve-fitting analysis of amide I profile, could be caused inter alia by air pollutants. Alterations in protein structure and in their content in the pollen may increase the sensitization and subsequent risk of allergy in predisposed people. The obtained results suggest that the changes in chemical composition of pollen may be a good indicator of air quality and that FTIR may be successfully applied in biomonitoring.
Institute of Nuclear Physics Polish Academy of Sciences, 31342, Krakow, Poland.
J
J
Depciuch
Department of Environmental Biology, Faculty of Biology and Agriculture, University of Rzeszow, Zelwerowicza 4, 35-601, Rzeszow, Poland. idalia@univ.rzeszow.pl.
I
I
Kasprzyk
Institute of Nuclear Physics Polish Academy of Sciences, 31342, Krakow, Poland.
E
E
Roga
Institute of Nuclear Physics Polish Academy of Sciences, 31342, Krakow, Poland.
M
M
Parlinska-Wojtan
D000393:Air Pollutants / Q000032:analysis; D000397:Air Pollution / Q000032:analysis; D000485:Allergens / Q000737:chemistry / Q000648:ultrastructure; D019306:Artemisia / Q000737:chemistry* / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D011058:Pollen / Q000737:chemistry* / Q000648:ultrastructure*; D017550:Spectroscopy, Fourier Transform Infrared
D000393:Air Pollutants; D000485:Allergens
Allergenic pollen; Artemisia; Biomonitoring; FTIR spectroscopy; Protein secondary structure; Traffic pollutants