Inhibitory effect of 1,25-dihydroxyvitamin D3on Porphyromonas gingivalis-induced inflammation and bone resorption in vivo.
OBJECTIVE
To investigate whether intragastric administration of 1,25-dihydroxyvitamin D3(1,25(OH)2D3) could inhibit the bone resorption and inflammation in a mouse calvarial model infected by Porphyromonas gingivalis (P. gingivalis).


METHODS
Live P. gingivalis ATCC 33277 was injected once daily for 6days into the subcutaneous tissue overlying the calvaria in mice. At the same time, 1,25(OH)2D3(50μg/kg per day) was administered by gavage for 9days, starting 3d before the infection. Mice were killed under ether anesthesia 8h after the last injection of P. gingivalis. Micro-computed tomography scanning was used to evaluate calvarial bone loss. Tartrate-resistant acid phosphatase was used to detect osteoclast activity. Real-time PCR was used to assess the mRNA expressions of OPG, RANKL, c-Fos, NFATc1, CTSK and TRAP in calvarial bone and IL-6, IL-10, IL-1β, IL-12p40 and TNF-α in soft tissue. The levels of serum IL-6, IL-10 were determined by ELISA.


RESULTS
1,25(OH)2D3treatment apparently attenuated bone resorption in P. gingivalis-induced mouse calvarial model and markedly reduced the number of osteoclasts. The expression levels of RANKL and osteoclast-related genes such as c-Fos, NFATc1, CTSK and TRAP were also decreased by 1,25(OH)2D3. Besides, 1,25(OH)2D3inhibited the expression of pro-inflammatory cytokines IL-6, IL-12p40 and TNF-α and enormously elevated the expression of anti-inflammatory cytokine IL-10.


CONCLUSIONS
1,25(OH)2D3may decrease bone resorption in vivo via suppressing the expression of osteoclast-related genes and its anti-inflammatory properties.
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine of Ministry of Education (KLOBM), School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
Zhao-Fei
ZF
Li
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine of Ministry of Education (KLOBM), School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
Li-Hua
LH
Cao
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine of Ministry of Education (KLOBM), School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
Ying
Y
Wang
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine of Ministry of Education (KLOBM), School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
Zhou
Z
Zhang
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine of Ministry of Education (KLOBM), School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. Electronic address: fmwmail@whu.edu.cn.
Ming-Wen
MW
Fan
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine of Ministry of Education (KLOBM), School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China. Electronic address: xuqingan1977@aliyun.com.
Qing-An
QA
Xu
D000818:Animals; D001862:Bone Resorption / Q000378:metabolism* / Q000382:microbiology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects; D007249:Inflammation / Q000378:metabolism / Q000382:microbiology; D018836:Inflammation Mediators / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010010:Osteoclasts / Q000187:drug effects; D016966:Porphyromonas gingivalis / Q000187:drug effects*; D053245:RANK Ligand / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012886:Skull / Q000187:drug effects* / Q000382:microbiology; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000494:pharmacology; D055114:X-Ray Microtomography
D016207:Cytokines; D018836:Inflammation Mediators; D053245:RANK Ligand; D014807:Vitamin D; C097949:1,25-dihydroxyvitamin D
1,25-dihydroxyvitamin D(3); Bone resorption; Inflammation; Porphyromonas gingivalis