Assessment of the in vitro bioactive properties of lactic acid bacteria isolated from native ecological niches of Ecuador.
Lactic acid bacteria are known for their biotechnological potential. In various regions of Ecuador numerous indigenous biological resources are largely undocumented. In this study, we evaluated the potential probiotic characteristics and antagonistic in vitro properties of some lactic acid bacteria from native niches of the subtropical rain forests of Ecuador. These isolates were identified according to their morphological properties, standard API50CH fermentation profile and RAPD-DNA polymorphism pattern. The selected isolates were further evaluated for their probiotic potential. The isolates grew at 15°C and 45°C, survived at a pH ranging from 2.5 to 4.5 in the presence of 0.3% bile (>90%) and grew under sodium chloride conditions. All selected isolates were sensitive to ampicillin, amoxicillin and cefuroxime and some showed resistance to gentamicin, kanamycin and tetracycline. Moreover, the agar well diffusion assay showed that the supernatant of each strain at pH 3.0 and pH 4.0, but not at pH 7.0 exhibited increased antimicrobial activity (inhibition zone >15mm) against two foodborne pathogens, Escherichia coli and Salmonella spp. To our knowledge, this is the first report describing the antagonistic activity against two foodborne pathogens and the probiotic in vitro potential of lactic acid bacteria isolated from native biota of Ecuador.
Faculty of Engineering in Agricultural and Environmental Sciences, Technical University of the North, Ibarra, Ecuador.
Ana B
AB
Benavides
Faculty of Engineering in Agricultural and Environmental Sciences, Technical University of the North, Ibarra, Ecuador.
Mario
M
Ulcuango
Faculty of Engineering in Agricultural and Environmental Sciences, Technical University of the North, Ibarra, Ecuador.
Lucía
L
Yépez
Faculty of Engineering in Agricultural and Environmental Sciences, Technical University of the North, Ibarra, Ecuador. Electronic address: gntenea@utn.edu.ec.
Gabriela N
GN
Tenea
D000898:Antibiosis; D015373:Bacterial Typing Techniques; D004352:Drug Resistance, Microbial; D004484:Ecuador; D005285:Fermentation; D035264:Flowers / Q000382:microbiology; D005516:Food Microbiology; D005638:Fruit / Q000382:microbiology; D007777:Lactobacillaceae / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D010944:Plants / Q000382:microbiology*; D019936:Probiotics; D065947:Rainforest; D019105:Random Amplified Polymorphic DNA Technique; D055049:Salt Tolerance; D013045:Species Specificity; D013696:Temperature
Actividad antimicrobiana; Antimicrobial activity; Bacterias ácido lácticas; Food-borne pathogens; Lactic acid bacteria; Patógenos alimentarios; Potencial probiótico; Probiotic potential