Rapid Bacterial Detection via an All-Electronic CMOS Biosensor.
The timely and accurate diagnosis of infectious diseases is one of the greatest challenges currently facing modern medicine. The development of innovative techniques for the rapid and accurate identification of bacterial pathogens in point-of-care facilities using low-cost, portable instruments is essential. We have developed a novel all-electronic biosensor that is able to identify bacteria in less than ten minutes. This technology exploits bacteriocins, protein toxins naturally produced by bacteria, as the selective biological detection element. The bacteriocins are integrated with an array of potassium-selective sensors in Complementary Metal Oxide Semiconductor technology to provide an inexpensive bacterial biosensor. An electronic platform connects the CMOS sensor to a computer for processing and real-time visualization. We have used this technology to successfully identify both Gram-positive and Gram-negative bacteria commonly found in human infections.
Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON, M5S 3G4, Canada.
Nasim
N
Nikkhoo
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.|Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.
Nichole
N
Cumby
Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON, M5S 3G4, Canada.
P Glenn
PG
Gulak
Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.
Karen L
KL
Maxwell
D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism; D001430:Bacteriocins / Q000378:metabolism*; D015374:Biosensing Techniques; D004581:Electronics; D008670:Metals / Q000737:chemistry*; D011188:Potassium / Q000378:metabolism; D012666:Semiconductors
D001430:Bacteriocins; D008670:Metals; D011188:Potassium