Identification of coagulase-negative Staphylococcus saprophyticus by polymerase chain reaction based on the heat-shock repressor encoding hrcA gene.
Staphylococcus saprophyticus is an uropathogen belonging to the human microbiota and is responsible for community-acquired infections of the urinary tract. Identification of Staphylococcus species by biochemical tests is laborious and costly when compared to routine laboratory tests. Because of their high sensitivity and specificity, molecular methods are better suited for accurate identification of Staphylococcusspp. Therefore, the goal of this work was to standardize a polymerase chain reaction (PCR) protocol using species-specific primers, based on the heat-shock repressor coding hrcA gene, for the identification of S.saprophyticus. A total of 142 S. saprophyticus strains were obtained from different sources, including clinical, environmental, and foodborne strains. We also included 98 strains of Staphylococcus spp. to further validate the proposed method. Reliable results for the detection of S. saprophyticus isolates were obtained for 100% of the strains evaluated. The results were in accordance with matrix-assisted laser desorption ionization-time of flight mass spectrometry identification, thus highlighting the applicability of species-specific PCR for the molecular identification of S. saprophyticus.
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde (CCS), Av. Carlos Chagas Filho, 373, Bloco I, Cidade Universitária, 21941-920Rio de Janeiro, RJ, Brazil.
Weslley de
W
Paiva-Santos
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde (CCS), Av. Carlos Chagas Filho, 373, Bloco I, Cidade Universitária, 21941-920Rio de Janeiro, RJ, Brazil.
Elaine M
EM
Barros
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde (CCS), Av. Carlos Chagas Filho, 373, Bloco I, Cidade Universitária, 21941-920Rio de Janeiro, RJ, Brazil.
Viviane Santos de
VS
Sousa
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde (CCS), Av. Carlos Chagas Filho, 373, Bloco I, Cidade Universitária, 21941-920Rio de Janeiro, RJ, Brazil.
Marinella Silva
MS
Laport
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde (CCS), Av. Carlos Chagas Filho, 373, Bloco I, Cidade Universitária, 21941-920Rio de Janeiro, RJ, Brazil. Electronic address: marciagm@micro.ufrj.br.
Marcia
M
Giambiagi-deMarval
D004783:Environmental Microbiology; D005260:Female; D005516:Food Microbiology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology; D012097:Repressor Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology; D057790:Staphylococcus saprophyticus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014552:Urinary Tract Infections / Q000382:microbiology
D012097:Repressor Proteins
Identification; PCR; Staphylococcus saprophyticus; hrcA