Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients.
The aim of this work was to document molecular epidemiology of Rasamsonia argillacea species complex isolates from cystic fibrosis (CF) patients. In this work, 116 isolates belonging to this species complex and collected from 26 CF patients and one patient with chronic granulomatous disease were characterized using PCR amplification assays of repetitive DNA sequences and electrophoretic separation of amplicons (rep-PCR). Data revealed a clustering consistent with molecular species identification. A single species was recovered from most patients. Rasamsonia aegroticola was the most common species, followed by R. argillacea sensu stricto and R. piperina, while R. eburnea was not identified. Of 29 genotypes, 7 were shared by distinct patients while 22 were patient specific. In each clinical sample, most isolates exhibited an identical genotype. Genotyping of isolates recovered from sequential samples from the same patient confirmed the capability of R. aegroticola and R. argillacea isolates to chronically colonize the airways. A unique genotype was recovered from two siblings during a 6-month period. In the other cases, a largely dominant genotype was detected. Present results which support the use of rep-PCR for both identification and genotyping for the R. argillacea species complex provide the first molecular evidence of chronic airway colonization by these fungi in CF patients.
L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, Angers, France.|University Moulay Ismail, Faculty of Sciences, Meknes, Morocco.
Abdelmounaim
A
Mouhajir
Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France.|Normandie University, UNIROUEN, Rouen, France.
Olivier
O
Matray
L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, Angers, France.
Sandrine
S
Giraud
Centre de Ressources et de Compétences en Mucoviscidose, Hôpital Renée Sabran, Giens, France.
Laurent
L
Mély
Centre de Ressources et de Compétences en Mucoviscidose, Service de Pédiatrie, Hôpital Charles Nicolle, Rouen, France.
Christophe
C
Marguet
Centre de Ressources et de Compétences en Mucoviscidose, Unité de Pédiatrie, Hôpital Necker-Enfants Malades, Paris, France.
Isabelle
I
Sermet-Gaudelus
L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, Angers, France.|Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Brest, France.
Solène
S
Le Gal
Laboratoire de Microbiologie, Hôpital Jacques Monod, Le Havre, France.
Franck
F
Labbé
Centre de Ressources et de Compétences en Mucoviscidose Adultes, Service de Pneumologie, Centre Hospitalier Universitaire, Angers, France.
Christine
C
Person
Centre de Ressources et de Compétences en Mucoviscidose Enfants, Service de Pédiatrie, Centre Hospitalier Universitaire, Angers, France.
Françoise
F
Troussier
Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France.
Jean-Jacques
JJ
Ballet
Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France.|Normandie University, UNIROUEN, Rouen, France.
Gilles
G
Gargala
University Moulay Ismail, Faculty of Sciences, Meknes, Morocco.
Rachid
R
Zouhair
Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire Necker-Enfants Malades, Paris, France.
Marie-Elisabeth
ME
Bougnoux
L'UNAM Université, Université d'Angers, Groupe d'Etude des Interactions Hôte-Pathogène, Angers, France.|Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Angers, France.
Jean-Philippe
JP
Bouchara
Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Rouen, France loic.favennec@chu-rouen.fr.|Normandie University, UNIROUEN, Rouen, France.
Loïc
L
Favennec
D016000:Cluster Analysis; D003550:Cystic Fibrosis / Q000150:complications*; D004586:Electrophoresis; D032641:Eurotiales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods; D017720:Molecular Epidemiology; D009181:Mycoses / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics