High Resolution Melting (HRM) applied to wine authenticity.
Wine authenticity methods are in increasing demand mainly in Denomination of Origin designations. The DNA-based methodologies are a reliable means of tracking food/wine varietal composition. The main aim of this work was the study of High Resolution Melting (HRM) application as a screening method for must and wine authenticity. Three sample types (leaf, must and wine) were used to validate the three developed HRM assays (Vv1-705bp; Vv2-375bp; and Vv3-119bp). The Vv1 HRM assay was only successful when applied to leaf and must samples. The Vv2 HRM assay successfully amplified all sample types, allowing genotype discrimination based on melting temperature values. The smallest amplicon, Vv3, produced a coincident melting curve shape in all sample types (leaf and wine) with corresponding genotypes. This study presents sensitive, rapid and efficient HRM assays applied for the first time to wine samples suitable for wine authenticity purposes.
University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal; University of Lisboa, Faculty of Sciences, BioISI - Biosystems & Integrative Sciences Institute, Campo Grande, 1749-016 Lisboa, Portugal.
Leonor
L
Pereira
University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal; University of Lisboa, Faculty of Sciences, BioISI - Biosystems & Integrative Sciences Institute, Campo Grande, 1749-016 Lisboa, Portugal.
Sónia
S
Gomes
University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal; University of Lisboa, Faculty of Sciences, BioISI - Biosystems & Integrative Sciences Institute, Campo Grande, 1749-016 Lisboa, Portugal.
Cláudia
C
Castro
National Institute for Agricultural and Veterinary Research (INIAV), 2565-191 Dois Portos, Portugal.
José Eduardo
JE
Eiras-Dias
National Institute for Agricultural and Veterinary Research (INIAV), 2565-191 Dois Portos, Portugal.
João
J
Brazão
SOGRAPE VINHOS S.A., 4430-809 Avintes, Portugal.
António
A
Graça
University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal; INESC/TEC, Rua do Campo Alegre n. 687, 4169-007 Porto, Portugal.
José R
JR
Fernandes
University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal; University of Lisboa, Faculty of Sciences, BioISI - Biosystems & Integrative Sciences Institute, Campo Grande, 1749-016 Lisboa, Portugal. Electronic address: plopes@utad.pt.
Paula
P
Martins-Lopes
D018744:DNA, Plant / Q000235:genetics*; D005838:Genotype; D025202:Molecular Diagnostic Techniques; D009691:Nucleic Acid Denaturation; D018515:Plant Leaves / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D027843:Vitis / Q000145:classification / Q000235:genetics*; D014920:Wine / Q000032:analysis*
D018744:DNA, Plant
Authenticity; HRM; Must; Vitis vinifera L.; Wine