Lactobacillus sakei K17, an inducer of IL-10 expression in antigen-presenting cells, attenuates TNBS-induced colitis in mice.
To understand the anti-colitic effects of probiotics that up-regulate interleukin (IL)-10 expression in dendritic cells (DCs) and macrophages, we isolated Lactobacillus sakei K17, which potently induced IL-10 expression in DCs and peritoneal macrophages in vitro, among the lactic acid bacteria strains collected from kimchi and investigated its anti-inflammatory effect in mice with 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. Oral administration of K17 (2 × 109CFU·mouse-1·day-1) in mice with TNBS-induced colitis suppressed colon shortening and myeloperoxidase activity, as well as infiltration of CD86+cells into the colon. Treatment with K17 also increased TNBS-suppressed expression of tight junction proteins and IL-10, but inhibited activation of nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinases and expression of tumor necrosis factor α and IL-17. Its effect was comparable with that of sulfasalazine (50 mg/kg), a positive commercial ant-colitic drug. Furthermore, treatment with K17 (1 × 105CFU/mL) potently inhibited lipopolysaccharide (LPS)-stimulated NF-κB activation in DCs and peritoneal macrophages and restored tight junction protein expression in LPS-stimulated Caco-2 cells. These findings suggest that Lactobacillus sakei K17 may ameliorate colitis by up-regulating the expression of IL-10 and tight junction proteins and inhibiting NF-κB activation.
a Department of Life and Nanopharmaceutical Sciences, College of Pharmacy , Kyung Hee University , Seoul , Korea.
Su-Hyeon
SH
Eun
a Department of Life and Nanopharmaceutical Sciences, College of Pharmacy , Kyung Hee University , Seoul , Korea.
Su-Min
SM
Lim
a Department of Life and Nanopharmaceutical Sciences, College of Pharmacy , Kyung Hee University , Seoul , Korea.|b Department of Food and Nutrition , Kyung Hee University , Seoul , Korea.
Se-Eun
SE
Jang
b Department of Food and Nutrition , Kyung Hee University , Seoul , Korea.
Myung Joo
MJ
Han
a Department of Life and Nanopharmaceutical Sciences, College of Pharmacy , Kyung Hee University , Seoul , Korea.
Dong-Hyun
DH
Kim
Colitis; IL-10; Lactobacillus sakei; dendritic cells; inflammatory bowel disease; macrophages