Amphotericin B Inhibits Enterovirus 71 Replication by Impeding Viral Entry.
Enterovirus 71 (EV71) infection causes hand-foot-and-mouth disease that leads to cardiopulmonary complications and death in young children. There is thus an urgent need to find new treatments to control EV71 infection. In this study, we report potent inhibition of EV71 by a polyene antibiotic Amphotericin B. Amphotericin B profoundly diminished the expression of EV71 RNA and viral proteins in the RD cells and the HEK293 cells. As a result, EV71 production was inhibited by Amphotericin B with an EC50 (50% effective concentration) of 1.75 μM in RD cells and 0.32 μM in 293 cells. In addition to EV71, EV68 was also strongly inhibited by Amphotericin B. Results of mechanistic studies revealed that Amphotericin B targeted the early stage of EV71 infection through impairing the attachment and internalization of EV71 by host cells. As an effective anti-fungi drug, Amphotericin B thus holds the promise of formulating a novel therapeutic to treat EV71 infection.
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Fengwen
F
Xu
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Xiaoxiao
X
Zhao
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Siqi
S
Hu
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Jian
J
Li
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Lijuan
L
Yin
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Shan
S
Mei
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Tingting
T
Liu
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Ying
Y
Wang
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Lili
L
Ren
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Shan
S
Cen
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Zhendong
Z
Zhao
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Jianwei
J
Wang
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Qi
Q
Jin
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.|Lady Davis Institute, Jewish General Hospital, Montreal, Qc, Canada H3T 1E2.
Chen
C
Liang
Department of Medical Oncology, Beijing Hospital, Beijing, P. R. China.
Bin
B
Ai
MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for AIDS Research, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, P. R. China.
Fei
F
Guo
D000666:Amphotericin B / Q000494:pharmacology*; D045744:Cell Line, Tumor; D029821:Enterovirus A, Human / Q000502:physiology*; D004769:Enterovirus Infections / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D053586:Virus Internalization / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects*
D000666:Amphotericin B