Elemental Mass Spectrometry for Absolute Intact Protein Quantification without Protein-Specific Standards: Application to Snake Venomics.
Absolute protein quantification methods based on molecular mass spectrometry usually require stable isotope-labeled analogous standards for each target protein or peptide under study, which in turn must be certified using natural standards. In this work, we report a direct and accurate methodology based on capLC-ICP-QQQ and online isotope dilution analysis for the absolute and sensitive quantification of intact proteins. The combination of the postcolumn addition of34S and a generic S-containing internal standard spiked to the sample provides full compound independent detector response and thus protein quantification without the need for specific standards. Quantitative recoveries, using a chromatographic core-shell C4 column for the various protein species assayed were obtained (96-100%). Thus, the proposed strategy enables the accurate quantification of proteins even if no specific standards are available for them. In addition, to the best of our knowledge, we obtained the lowest detection limits reported in the quantitative analysis of intact proteins by direct measurement of sulfur with ICPMS (358 fmol) and protein (ranging from 7 to 15 fmol depending on the assayed protein). The quantitative results for individual and simple mixtures of model proteins were statistically indistinguishable from the manufacturer's values. Finally, the suitability of the strategy for real sample analysis (including quantitative protein recovery from the column) was illustrated for the individual absolute quantification of the proteins and whole protein content in a venom sample. Parallel capLC-ESI-QTOF analysis was employed to identify the proteins, a prerequisite to translate the mass of quantified S for each chromatographic peak into individual protein mass.
Department of Physical and Analytical Chemistry, University of Oviedo , Julián Clavería 8, 33006 Oviedo, Spain.
Francisco
F
Calderón-Celis
Department of Physical and Analytical Chemistry, University of Oviedo , Julián Clavería 8, 33006 Oviedo, Spain.
Silvia
S
Diez-Fernández
Department of Physical and Analytical Chemistry, University of Oviedo , Julián Clavería 8, 33006 Oviedo, Spain.
José Manuel
JM
Costa-Fernández
Department of Physical and Analytical Chemistry, University of Oviedo , Julián Clavería 8, 33006 Oviedo, Spain.
Jorge Ruiz
JR
Encinar
Instituto de Biomedicina de Valencia , Consejo Superior de Investigaciones Científicas (CSIC), Jaume Roig 11, 46010 Valencia, Spain.
Juan J
JJ
Calvete
Department of Physical and Analytical Chemistry, University of Oviedo , Julián Clavería 8, 33006 Oviedo, Spain.
Alfredo
A
Sanz-Medel
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002417:Cattle; D045304:Cytochromes c / Q000032:analysis* / Q000378:metabolism; D004546:Elapid Venoms / Q000032:analysis*; D017815:Elapidae; D013058:Mass Spectrometry; D012710:Serum Albumin, Bovine / Q000032:analysis*; D014168:Transferrin / Q000032:analysis*
D000911:Antibodies, Monoclonal; D004546:Elapid Venoms; D014168:Transferrin; D012710:Serum Albumin, Bovine; D045304:Cytochromes c