Bombyx mori nucleopolyhedrovirus BM5 protein regulates progeny virus production and viral gene expression.
Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mori cultured cells and larvae, although viral genome replication was not affected. Bm5 disruption also caused aberrant expression of various viral genes at the very late stage of infection. Immunocytochemical analysis revealed that BM5 localized to the nuclear membrane. We also found that DUF3627 is important for OB production, transcriptional regulation of viral genes, and subcellular localization of BM5. Compared with wild-type BmNPV infection, larval death was delayed when B. mori larvae were infected with Bm5 mutants. These results suggest that BM5 is involved in progeny virus production and regulation of viral gene expression at the very late stage of infection.
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan. Electronic address: kokusho@ss.ab.a.u-tokyo.ac.jp.
Ryuhei
R
Kokusho
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan.
Yoshikazu
Y
Koh
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan.
Masaru
M
Fujimoto
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan.
Toru
T
Shimada
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan. Electronic address: katsuma@ss.ab.a.u-tokyo.ac.jp.
Susumu
S
Katsuma
D000818:Animals; D012831:Bombyx / Q000821:virology*; D002460:Cell Line; D002478:Cells, Cultured; D004261:DNA Replication; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D054884:Host-Pathogen Interactions / Q000235:genetics; D042541:Intracellular Space; D007814:Larva; D009154:Mutation; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D021381:Protein Transport; D011993:Recombinant Fusion Proteins; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D011993:Recombinant Fusion Proteins; D014764:Viral Proteins
Baculovirus; Bm5; BmN cells; BmNPV; Bombyx mori larvae; DUF3627; Progeny production; Subcellular localization; Viral gene expression