Increasing the miR-126 expression in the peripheral blood of patients with diabetic foot ulcers treated with maggot debridement therapy.
miR-126 may increase angiogenesis in patients with diabetic foot ulcers (DFUs) treated with maggot debridement therapy (MDT).



Real-time quantitative PCR was used to detect expression of miR-126 mRNA in the peripheral blood among the non-diabetic population, type 2 diabetes mellitus patients without DFU, and patients with DFUs of type 2 diabetes mellitus. The expression of miR-126 mRNA in the peripheral blood of patients with DFUs was observed before and after MDT. Finally, human umbilical vein endothelial cells (HUVEC) were utilized to explore miR-126 mRNA expression with maggot excretions/secretions (ES).



In the patients with DFUs, the miR-126 mRNA expression level in the peripheral blood was less than that type 2 diabetes mellitus patients without DFU, and much lower than that in the non-diabetic population (P<0.001). The miR-126 expression level was significantly increased in those DFU patients treated with MDT (P<0.05). Finally, using HUVEC co-cultured with ES, we showed the ES increased miR-126 expression in vitro (P<0.001).



MDT upregulates the miR-126 expression in the peripheral blood of patients with DFUs.
The 454th Hospital of Chinese PLA, Nanjing Road Street Baixia District No.1, Nanjing 210002, China. Electronic address: xiaopengyou1226@sina.com.
Jie
J
Zhang
The 454th Hospital of Chinese PLA, Nanjing Road Street Baixia District No.1, Nanjing 210002, China. Electronic address: 27966509@qq.com.
Xin-Juan
XJ
Sun
The 454th Hospital of Chinese PLA, Nanjing Road Street Baixia District No.1, Nanjing 210002, China. Electronic address: cysc111@163.com.
Jin'an
J
Chen
The 454th Hospital of Chinese PLA, Nanjing Road Street Baixia District No.1, Nanjing 210002, China. Electronic address: huzhiwei_yu1208@163.com.
Zhi Wei
ZW
Hu
The 454th Hospital of Chinese PLA, Nanjing Road Street Baixia District No.1, Nanjing 210002, China. Electronic address: 872819805@qq.com.
Lei
L
Wang
The 454th Hospital of Chinese PLA, Nanjing Road Street Baixia District No.1, Nanjing 210002, China.
Dong Mei
DM
Gu
The 454th Hospital of Chinese PLA, Nanjing Road Street Baixia District No.1, Nanjing 210002, China. Electronic address: wap454hospital@hotmail.com.
Ai-Ping
AP
Wang
D000368:Aged; D000818:Animals; D001691:Biological Therapy / Q000379:methods*; D012634:Bodily Secretions / Q000502:physiology; D002478:Cells, Cultured; D002681:China; D018920:Coculture Techniques; D000529:Complementary Therapies; D003646:Debridement / Q000379:methods*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D017719:Diabetic Foot / Q000097:blood / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D004175:Diptera / Q000502:physiology*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D005856:Germ-Free Life; D006786:Hospitals, Urban; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007814:Larva / Q000502:physiology; D008297:Male; D035683:MicroRNAs / Q000819:agonists* / Q000097:blood / Q000378:metabolism; D008875:Middle Aged; D015854:Up-Regulation
C527111:MIRN126 microRNA, human; D035683:MicroRNAs
Diabetic foot ulcer; Expression; Maggot debridement therapy; miR-126; miRNA