Direct detection of Helicobacter pylori in biopsy specimens using a high-throughput multiple genetic detection system.
We evaluated the direct high-throughput multiple genetic detection system (dHMGS) for Helicobacter pylori in gastric biopsies.



One hundred and thirty-three specimens were concurrently analyzed by dHMGS, rapid urease test, culture and sequencing.



dHMGS was highly sensitive and specific for H. pylori identification compared with culture and rapid urease test. The correlation coefficient of the quantitative standard curve was R2= 0.983. A significant difference in the relative H. pylori DNA abundance was found in different gastroduodenal diseases. Concordance rates between dHMGS and sequencing for resistance mutations were 97.1, 100.0, 85.3 and 97.1%, respectively. Finally, dHMGS could efficiently distinguish mixed infection in biopsy specimens.



The dHMGS could efficiently diagnose and quantify H. pylori burden in biopsies, simultaneously screening for virulence, antibiotic resistance and presence of the multistrain infections.
Department of Laboratory Medicine, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.|Key Laboratory of Clinical Geriatric Medicine, Shanghai 200040, China.|Research Center on Aging & Medicine, Fudan University, Shanghai 200040, China.
Yanmei
Y
Zhang
Department of Laboratory Medicine, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.
Shiwen
S
Wang
Department of Laboratory Medicine, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.
Binjie
B
Hu
Department of Laboratory Medicine, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.
Fuju
F
Zhao
Department of Endoscopy, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.
Ping
P
Xiang
Department of Endoscopy, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.
Danian
D
Ji
Department of Laboratory Medicine, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.
Fei
F
Chen
Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai 200032, China.
Xiaoli
X
Liu
Department of Laboratory Medicine, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.
Feng
F
Yang
Ningbo HEALTH Gene Technologies Co., Ltd, Ningbo 315000, PR China.
Yong
Y
Wu
Ningbo HEALTH Gene Technologies Co., Ltd, Ningbo 315000, PR China.
Mimi
M
Kong
Ningbo HEALTH Gene Technologies Co., Ltd, Ningbo 315000, PR China.
Li
L
Nan
Department of Laboratory Medicine, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.
Yingxin
Y
Miao
Department of Laboratory Medicine, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.
Wenrong
W
Jiang
Department of Laboratory Medicine, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.
Yi
Y
Fang
Department of Laboratory Medicine, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.
Jinghao
J
Zhang
Department of Gastroenterology, Gerontology Institute of Shanghai affiliated to Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.
Zhijun
Z
Bao
Department of Internal Medicine, Division of Pulmonary & Critical Care Medicine, University of Michigan Medical School & Veterans' Affairs Ann Arbor Health System, Ann Arbor, MI 48105, USA.
Michal A
MA
Olszewski
Department of Laboratory Medicine, Huadong Hospital affiliated to Fudan University, No. 221 Yanan West Road, Shanghai 200040, China.|Key Laboratory of Clinical Geriatric Medicine, Shanghai 200040, China.|Research Center on Aging & Medicine, Fudan University, Shanghai 200040, China.
Hu
H
Zhao
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001706:Biopsy; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014510:Urease / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D014510:Urease
Helicobacter pylori; direct high-throughput multiple genetic detection system; gastric biopsy specimens; identification; mixed infection; quantitative analysis; resistance; virulence