Associations of human leucocyte antigen class II-DQB1 alleles with hepatitis C virus infection in Egyptian population: a multicentre family-based study.
Hepatitis C infection is a global pandemic. HLA-DQB1 alleles are believed to have an effective role in immune response against HCV including susceptibility to or protection from this infection. The aim of this study was to investigate the contribution of HLA-DQB1 alleles in the outcome of HCV genotype-4 infection through a family-based association study. Egyptian families with HCV (324) were recruited for this study (324 index positive for RNA-HCV, 225 positive relatives representing chronic hepatitis C cases and 582 family members negative for HCV-RNA [control], 63 of whom spontaneously cleared the virus. All subjects were genotyped for HLA-DQB1 alleles by sequence-specific primers (SSP-PCR) and sequence-based typing (SBT) methods. The frequency of DQB1*02:01:01 carriage was significantly higher in infected patients when compared to controls and those who spontaneously cleared virus (OR=5.47, P<.0001 and OR= 6.5234, P<.0001, respectively), and the carriage of the DQB1*03:01:01:01 allele was significantly higher in those who cleared and controls when compared to the infected patients (OR=0.2889, P<.0001 and OR=0.3016, P<.0001, respectively). On the other hand, the frequency of DQB1*06:01:01 and QB1*05:01:01:01 alleles was not associated with infection (comparison of infected and cleared patients showed OR of 2.1598 [P<.01]), but it becomes nonsignificant after adjustments with the Bonferroni formula (PC>0.05) and OR= 1.3523, P>.05, respectively. This study shows that clearance of HCV is associated with DQB1*03:01:01:01 allele and chronicity of HCV infection associated with the risk allele: DQB1*02:01:01.
Tropical Medicine & Hepatology, Mansoura Faculty of Medicine, Mansoura, Egypt.
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El-Bendary
Medical Biochemistry, Mansoura Faculty of Medicine, Mansoura, Egypt.
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Neamatallah
Tropical Medicine & Hepatology, Cairo Faculty of Medicine, Cairo, Egypt.
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Esmat
Public Health & Preventive Medicine Department, Mansoura Faculty of Medicine, Mansoura, Egypt.
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Kamel
Tropical Medicine & Hepatology, Mansoura Faculty of Medicine, Mansoura, Egypt.
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Elalfy
Tropical Medicine & Hepatology, Mansoura Faculty of Medicine, Mansoura, Egypt.
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Besheer
Biochemistry, Faculty of Science, Mansoura University, Mansoura, Egypt.
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Eldeib
Internal Medicine Department, Alazhar Faculty of Medicine- Assiut University, Assiut, Egypt.
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Eladl
Department of Community, Environmental and Occupational Medicine, Ain Shams Faculty of Medicine, Cairo, Egypt.
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El-Setouhy
Public Health & Preventive Medicine Department, Mansoura Faculty of Medicine, Mansoura, Egypt.
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El-Gilany
Biochemistry, Faculty of Science, Mansoura University, Mansoura, Egypt.
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El-Waseef
D000328:Adult; D000368:Aged; D000483:Alleles; D004534:Egypt; D005260:Female; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D059866:HLA-DQ beta-Chains / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen

HCV
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HLA
; DNA sequencing; DQB1; allele; gene polymorphism; high resolution; intrafamilial; persistence; viral clearance