Implications of glycolytic and pentose phosphate pathways on the oxidative status and active mitochondria of the porcine oocyte during IVM.
Glycolysis and the pentose phosphate pathway (PPP) were modulated in porcine cumulus-oocyte complexes during IVM by the addition of inhibitors and stimulators of key enzymes of the pathways to analyze their influence on the oxidative status, active mitochondria, and maturation of the oocyte. The influence of pharmacologic and physiological inhibitors of glycolysis (Sodium fluoride and ATP) and PPP (6-Aminonicotinamide and nicotinamide adenine dinucleotide phosphate) was validated by assessing glucose and lactate turnover and brilliant cresyl blue staining in oocytes. Inhibitors of glycolysis and PPP activity significantly perturbed nuclear maturation, oxidative metabolism (Redox Sensor Red CC-1), and active mitochondria (Mitotracker Green FM) within oocytes (P < 0.05). In comparison, physiological stimulators of glycolysis (adenosine monophosphate) and PPP (nicotinamide adenine dinucleotide phosphate) did not affect any of evaluated parameter. In the absence of modulators, fluctuations in the oocyte oxidative activity and active mitochondria were observed during porcine IVM. The inhibition of glycolysis and PPP modified the pattern of oxidation and mitochondrial fluctuation, resulting in impaired meiotic progression. We demonstrated the relationship between carbohydrate metabolism in COC and oocyte redox status necessary for porcine oocyte IVM.
Area of Biochemistry, INITRA (Institute of Research and Technology on Animal Reproduction), Executing Unit INPA (Research in Animal Production) UBA-CONICET, School of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina. Electronic address: galvarez@fvet.uba.ar.
Gabriel Martín
GM
Alvarez
Area of Biochemistry, INITRA (Institute of Research and Technology on Animal Reproduction), Executing Unit INPA (Research in Animal Production) UBA-CONICET, School of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina.
Sebastián
S
Casiró
Area of Biochemistry, INITRA (Institute of Research and Technology on Animal Reproduction), Executing Unit INPA (Research in Animal Production) UBA-CONICET, School of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina.
Cynthia
C
Gutnisky
Area of Biochemistry, INITRA (Institute of Research and Technology on Animal Reproduction), Executing Unit INPA (Research in Animal Production) UBA-CONICET, School of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina.
Gabriel Carlos
GC
Dalvit
Robinson Research Institute, School of Paediatrics and Reproductive Health, Australian Research Council Center of Excellence for Nanoscale BioPhotonics, Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, South Australia, Australia.
Melanie L
ML
Sutton-McDowall
Robinson Research Institute, School of Paediatrics and Reproductive Health, Australian Research Council Center of Excellence for Nanoscale BioPhotonics, Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, South Australia, Australia.
Jeremy G
JG
Thompson
Area of Biochemistry, INITRA (Institute of Research and Technology on Animal Reproduction), Executing Unit INPA (Research in Animal Production) UBA-CONICET, School of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina.
Pablo Daniel
PD
Cetica
D000818:Animals; D054885:Cumulus Cells / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000502:physiology*; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D008928:Mitochondria / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D010427:Pentose Phosphate Pathway / Q000502:physiology*; D013552:Swine / Q000502:physiology*
D005947:Glucose
Glycolysis; Mitochondria; Oocyte; Oxidative status; Pentose phosphate pathway; Pig