S100B suppresses the differentiation of C3H/10T1/2 murine embryonic mesenchymal cells into osteoblasts.
S100 calcium-binding protein B (S100B) is expressed and released by adipocytes, and is positively correlated with body mass index, however, the direct effects of S100B on adipocytes remain unclear. Bone marrow‑derived mesenchymal stem cells have the capacity to differentiate into osteoblasts and adipocytes, which is important for bone metabolism. The current study aimed to determine the effect of S100B on adipogenesis and osteogenesis. The mouse embryo cell line C3H/10T1/2 was used to build cell models with varying levels of S100B protein expression. Western blot analysis was performed to assess the expression of various marker proteins. Oil red O staining and alizarin red S staining were used to detect adipogenesis and osteogenesis, respectively. S100B overexpression was associated with a significant increase in oil red O staining and a significant reduction in alizarin red S staining. Runt‑related transcription factor‑2 and bone morphogenetic protein 2 expression levels were significantly increased in the S100B underexpression group, however not in the S100B overexpression group. By contrast, the expression levels of the adipogenesis markers peroxisome proliferator‑activated receptor γ and CCAAT‑enhancer‑binding protein α was significantly increased in the S100B overexpression group, however not in the S100B underexpression group. Osteogenesis stimulation increased extracellular signal‑regulated kinase (ERK) phosphorylation, and adipogenesis stimulation increased c‑Jun N‑terminal kinase (JNK) phosphorylation. The results suggest that S100B inhibits osteogenesis, however stimulates adipogenesis. The ERK pathway is involved in the regulation of osteogenesis, whereas the JNK pathway is involved in the regulation of adipogenesis.
Department of Orthopedics, Jiangsu Province Hospital of TCM, Affiliated Hospital of Nanjing University of TCM, Nanjing, Jiangsu 210029, P.R. China.
Dong
D
Li
Department of Orthopedics, Jiangsu Province Hospital of TCM, Affiliated Hospital of Nanjing University of TCM, Nanjing, Jiangsu 210029, P.R. China.
Kaihua
K
Li
Department of Orthopedics, Jiangsu Province Hospital of TCM, Affiliated Hospital of Nanjing University of TCM, Nanjing, Jiangsu 210029, P.R. China.
Gang
G
Chen
Department of Orthopedics, Jiangsu Province Hospital of TCM, Affiliated Hospital of Nanjing University of TCM, Nanjing, Jiangsu 210029, P.R. China.
Jianlong
J
Xia
Department of Orthopedics, Jiangsu Province Hospital of TCM, Affiliated Hospital of Nanjing University of TCM, Nanjing, Jiangsu 210029, P.R. China.
Ting
T
Yang
Department of Orthopedics, Jiangsu Province Hospital of TCM, Affiliated Hospital of Nanjing University of TCM, Nanjing, Jiangsu 210029, P.R. China.
Ping
P
Cai
Department of Orthopedics, Jiangsu Province Hospital of TCM, Affiliated Hospital of Nanjing University of TCM, Nanjing, Jiangsu 210029, P.R. China.
Chen
C
Yao
Department of Orthopedics, Benq Hospital, Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210019, P.R. China.
Yongjiang
Y
Yang
Department of Orthopedics, Benq Hospital, Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210019, P.R. China.
Shichang
S
Yan
The Laboratory Animal Center, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029; P.R. China.
Rihua
R
Zhang
Department of Orthopedics, Benq Hospital, Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210019, P.R. China.
Hui
H
Chen
D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D020935:MAP Kinase Signaling System; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D064568:S100 Calcium Binding Protein beta Subunit / Q000378:metabolism*
D064568:S100 Calcium Binding Protein beta Subunit; C579159:S100b protein, mouse