Cytotoxic activities of flavonoids from a traditional Mongolian medicinal herb Clematis aethusifolia Turcz.
In the course of our search for antitumour constituents from the traditional Mongolian medicinal herb Clematis aethusifolia Turcz., 11 flavonoids were isolated for the first time from the dried aerial parts of the plant by flash C18column chromatography, Sephadex LH-20 and reversed phase preparative HPLC. The planar structures of these flavonoids were established based on 1D and 2D NMR and high-resolution mass spectrometry. Compounds 1, 2, 4 and 5 showed moderate cytotoxicity against a panel of five human solid tumour cell lines, including A-375, a human melanoma cell line; SK-OV-3, a human ovarian cancer cell line; A549, a human lung cancer cell line; HCT-15, a human colorectal adenocarcinoma cell line; and SH-SY5Y, a human neuroblastoma cell line (with IC50values of 20-70 μM). The obtained cytotoxic apigenin and its derivatives may be useful as standard compounds for the quality control of the crude drug and its preparations.
a College of Pharmacy , Inner Mongolia Medical University , Hohhot , China.
Yanxi
Y
Chang
a College of Pharmacy , Inner Mongolia Medical University , Hohhot , China.
Ping
P
Zhang
b College of Basic Medical Sciences , Inner Mongolia Medical University , Hohhot , China.
Xuan
X
Zhang
a College of Pharmacy , Inner Mongolia Medical University , Hohhot , China.
Jianping
J
Chen
c Department of Biomedical Sciences , University of Veterinary Medicine , Vienna , Austria.
Wolf-Dieter
WD
Rausch
d College of Traditional Mongolian Medicine , Inner Mongolia Medical University , Hohhot , China.
A
A
Gula
a College of Pharmacy , Inner Mongolia Medical University , Hohhot , China.
Baoquan
B
Bao
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D039324:Clematis / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D056785:Medicine, Mongolian Traditional; D010946:Plants, Medicinal
D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids
Clematis aethusifolia; chemical constituents; cytotoxicity; flavonoids; traditional Mongolian medicine