A new case report of urinary tract infection due to KPC-3-producing klebsiella pneumoniae (ST258) in Spain.
OBJECTIVE
We describe the characterization of a new isolated in Spain of Klebsiella pneumoniae ST258 producing KPC-3, carbapenems non-susceptible, recovered from a sample of urine from a patient with urinary tract infection and no history of carbapenems exposure.


METHODS
After the isolation, identification of K. pneumoniae was performed by biochemical tests and mass spectrometry. The carbapenems susceptibility testing was performed by microdilution and E-test in cation-adjusted Mueller-Hinton. The study was completed by Rapidec® Carba NP. In order to determine the genetic basis of resistance to carbapenems we used Xpert® Carba-R for carbapenemase type and subtype was subsequently analyzed by amplification by PCR and sequencing.


RESULTS
We demonstrated by MLST that the strain belonged to the clone of high-risk ST258.


CONCLUSIONS
This is the first characterization, in our media, of a clinical isolated of K. pneumoniae ST258 producing KPC-3 and no history of carbapenems exposure.
Departamento de Microbiología, Facultad de Medicina, Universidad de Granada-ibs. Granada.
A
A
Sorlózano-Puerto
UGC de Medicina Interna. Complejo Hospitalario Universitario de Granada-ibs. Granada.
D
D
Esteva-Fernández
Laboratorio de Antibióticos, Servicio de Bacteriología, Centro Nacional de Microbiologia. Majadahonda. Madrid.
J
J
Oteo-Iglesias
Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada-ibs. Granada. España.
J M
JM
Navarro-Marí
Departamento de Microbiología, Facultad de Medicina, Universidad de Granada-ibs. Granada. Laboratorio de Microbiología, Complejo Hospitalario Universitario de Granada-ibs. Granada. España.
J
J
Gutiérrez-Fernández
D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000201:enzymology; D013030:Spain; D014552:Urinary Tract Infections / Q000382:microbiology*; D001618:beta-Lactamases / Q000096:biosynthesis
D001426:Bacterial Proteins; D001618:beta-Lactamases; C495220:beta-lactamase KPC-3, Klebsiella pneumoniae