Antimicrobial and Antioxidant Activity of Chitosan/Hydroxypropyl Methylcellulose Film-Forming Hydrosols Hydrolyzed by Cellulase.
The aim of this study was to evaluate the impact of cellulase (C) on the biological activity of chitosan/hydroxypropyl methylcellulose (CH/HPMC) film-forming hydrosols. The hydrolytic activity of cellulase in two concentrations (0.05% and 0.1%) was verified by determination of the progress of polysaccharide hydrolysis, based on viscosity measurement and reducing sugar-ends assay. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging effect, the ferric reducing antioxidant power (FRAP), and microbial reduction of Pseudomonas fluorescens, Yersinia enterocolitica, Bacillus cereus, and Staphylococcus aureus were studied. During the first 3 h of reaction, relative reducing sugar concentration increased progressively, and viscosity decreased rapidly. With increasing amount of enzyme from 0.05% to 0.1%, the reducing sugar concentration increased, and the viscosity decreased significantly. The scavenging effect of film-forming solutions was improved from 7.6% at time 0 and without enzyme to 52.1% for 0.1% cellulase after 20 h of reaction. A significant effect of cellulase addition and reaction time on antioxidant power of the tested film-forming solutions was also reported. Film-forming hydrosols with cellulase exhibited a bacteriostatic effect on all tested bacteria, causing a total reduction.
Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, 37 Chelmonskiego St., 51-630 Wrocław, Poland. anna.zimoch@up.wroc.pl.
Anna
A
Zimoch-Korzycka
Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, 37 Chelmonskiego St., 51-630 Wrocław, Poland. lukasz.bobak@up.wroc.pl.
Łukasz
Ł
Bobak
Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, 37 Chelmonskiego St., 51-630 Wrocław, Poland. andrzej.jarmoluk@up.wroc.pl.
Andrzej
A
Jarmoluk
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D044602:Cellulases / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology; D006868:Hydrolysis; D065347:Hypromellose Derivatives / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D016166:Free Radical Scavengers; D065347:Hypromellose Derivatives; D048271:Chitosan; D044602:Cellulases
antimicrobial properties; antioxidant properties; cellulase; chitosan; hydroxypropyl methylcellulose