Green and rapid synthesis of silver nanoparticles using Borago officinalis leaf extract: anticancer and antibacterial activities.
This study highlights the facile, reliable, cost effective, and ecofriendly synthesis of silver nanoparticles (AgNPs) using Borago officinalis leaves extract efficiently. The biosynthesis of AgNPs was verified by UV-Vis spectrum which showed the surface plasmon resonance (SPR) band at 422 nm. Transmission electron microscope (TEM) analysis revealed that the particles were spherical, hexagonal, and irregular in shape and had size ranging from 30 to 80 nm. The energy dispersive X-ray spectroscopy (EDX) and elemental mapping have displayed the purity and maximum distribution of silver in the AgNPs. The crystalline nature of AgNPs had been identified using X-ray diffraction (XRD) and selected area diffraction pattern (SAED). The particle size analysis revealed that the Z-average diameter of the AgNPs was 50.86 nm with polydispersity index (PDI) 0.136. Zeta potential analysis displayed the colloidal stability of AgNPs. This work also showed the efficacy of AgNPs against lung cancer cell lines (A549) and cervical cancer cell line (HeLa), in vitro. The AgNPs showed cytotoxicity to the A549 and HeLa cancer cell line at the concentrations 5 and 2 μg/ml. The AgNPs were also explored for the antibacterial activity including biofilm inhibition against pathogenic bacteria. The B. officinalis leaves extract can be used efficiently for green synthesis AgNPs. The biosynthesized AgNPs demonstrated potentials as anticancer and antibacterial agents. This work provides helpful insight into the development of new anticancer and antimicrobial agents.
a Department of Oriental Medicine Biotechnology, College of Life science , Kyung Hee University Global Campus , 1732 Deokyoungdaero, Giheung-gu, Yongin-si , Gyeonggi-do , Republic of Korea.
Hina
H
Singh
a Department of Oriental Medicine Biotechnology, College of Life science , Kyung Hee University Global Campus , 1732 Deokyoungdaero, Giheung-gu, Yongin-si , Gyeonggi-do , Republic of Korea.
Juan
J
Du
a Department of Oriental Medicine Biotechnology, College of Life science , Kyung Hee University Global Campus , 1732 Deokyoungdaero, Giheung-gu, Yongin-si , Gyeonggi-do , Republic of Korea.
Tae-Hoo
TH
Yi
D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D029683:Borago / Q000737:chemistry*; D055772:Green Chemistry Technology; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles; D051379:Mice; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D000067996:RAW 264.7 Cells; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D010936:Plant Extracts; D012834:Silver
Borago officinalis; Silver nanoparticles; antibacterial effect; anticancer; biofilm inhibition