Association between MDR1 gene polymorphisms and the risk of Crohn's disease in a cohort of Algerian pediatric patients.
The multi-drug resistance gene (MDR1) has raised increasing interest as a susceptibility gene for Crohn's disease (CD). The role of MDR1 single-nucleotide polymorphisms (SNPs) in the predisposition and behavior of CD in the pediatric population is still elusive. Here, we investigated whether SNPs in MDR1 are associated with CD in Algerian pediatric patients.



A case-control study was conducted enrolling 47 pediatric CD patients and 100 controls. All subjects were genotyped for the most common MDR1 SNPs (C3434T, C1236T, and G2677A/T) using PCR-RFLP method. We also explored the association between polymorphisms and clinical sub-phenotypes.



We have detected no significant association of C3435T SNP and pediatric CD. However, we observed a significantly higher frequency of the risk alleles, 1236T and 2677T/A among the CD patients compared to controls. Moreover, the risk allele 1236T was associated to a higher risk for resective surgery.



Our data suggest that the C1236T and G2677A/T SNPs in the MDR1 gene are associated with CD and the C1236T risk allele with a more severe course of disease in Algerian pediatric patients. Further analysis using larger patients group and functional studies would be interesting to elucidate the role of MDR1 gene in pediatric CD.Pediatric Research (2016); doi:10.1038/pr.2016.163.
Department of Cellular and Molecular Biology, Cytokines and NO Synthases -Immunity and Pathogeny Team, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, Algiers, Algeria.
Amira
A
Bouzidi
Department of Cellular and Molecular Biology, Cytokines and NO Synthases -Immunity and Pathogeny Team, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, Algiers, Algeria.
Hamida
H
Mesbah-Amroun
Department of Cellular and Molecular Biology, Cytokines and NO Synthases -Immunity and Pathogeny Team, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, Algiers, Algeria.
Aziza
A
Boukercha
Department of Pediatrics, Bologhine Hospital, Algiers, Algeria.
Fadila
F
Benhassine
Department of Pediatrics, University Hospital Center Mustapha Bacha, Algiers, Algeria.
Réda
R
Belboueb
Department of Pediatrics, University Hospital Center Lamine Debaghine, Algiers, Algeria.
Karima
K
Berkouk
Department of Pediatrics, University Hospital Center Issaad Hassani, Algiers, Algeria.
Wassila
W
Messadi
Department of Cellular and Molecular Biology, Cytokines and NO Synthases -Immunity and Pathogeny Team, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene, Algiers, Algeria.
Chafia
C
Touil-Boukoffa
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D000293:Adolescent; D000462:Algeria; D016022:Case-Control Studies; D002648:Child; D015331:Cohort Studies; D003424:Crohn Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B