Involvement of dehydrins in 24-epibrassinolide-induced protection of wheat plants against drought stress.
In this study, we performed a comparative analysis of the physiological and biochemical parameters of wheat cultivars with contrasting drought resistance, drought-resistant Omskaya 35 (O-35) and less drought-resistant Salavat Yulaev (SYu), during 7-day germination under drought stress simulated by 5% mannitol. In addition, we evaluated the effectiveness of pre-sowing seed treatment with 0.4 μM 24-epibrassinolide (EBR) used to increase the resistance of plants of both cultivars to drought stress. It was revealed that mannitol has caused significant changes in the hormonal balance of the plants of both cultivars, associated with abscisic acid (ABA) accumulation and decrease in the contents of indoleacetic acid (IAA) and cytokinins (CKs). It should be noted that more dramatic changes in the content of phytohormones were characteristic for seedlings of SYu cultivar, which was reflected in a stronger growth inhibition of these plants. Pretreatment with EBR mitigated the negative effect of drought on the hormonal status and growth of seedlings during their germination. Furthermore, we found that drought caused accumulation of dehydrin (DHN) proteins, especially of low molecular weight DHNs, whose abundance was 2.5 times greater in O-35 cultivar than in SYu plants. EBR-pretreated plants of both cultivars were characterized by the additional accumulation of DHNs, indicating their involvement in the development of the EBR-induced wheat drought resistance. The use of fluridone allowed us to demonstrate ABA-dependent and ABA-independent pathways of regulation of low molecular mass dehydrins accumulation by EBR in wheat plants of both cultivars under drought conditions.
Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, pr. Octyabrya 71, Ufa, 450054, Russia. Electronic address: shakirova@anrb.ru.
Farida
F
Shakirova
Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, pr. Octyabrya 71, Ufa, 450054, Russia.
Chulpan
C
Allagulova
Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, pr. Octyabrya 71, Ufa, 450054, Russia.
Dilara
D
Maslennikova
Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, pr. Octyabrya 71, Ufa, 450054, Russia.
Kristina
K
Fedorova
Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, pr. Octyabrya 71, Ufa, 450054, Russia.
Ruslan
R
Yuldashev
Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, pr. Octyabrya 71, Ufa, 450054, Russia.
Alsu
A
Lubyanova
Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, pr. Octyabrya 71, Ufa, 450054, Russia.
Marina
M
Bezrukova
Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, pr. Octyabrya 71, Ufa, 450054, Russia.
Azamat
A
Avalbaev
D000040:Abscisic Acid / Q000378:metabolism; D060406:Brassinosteroids / Q000494:pharmacology*; D003583:Cytokinins / Q000378:metabolism; D055864:Droughts; D007210:Indoleacetic Acids / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects; D014908:Triticum / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D060406:Brassinosteroids; D003583:Cytokinins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013260:Steroids, Heterocyclic; C070556:dehydrin proteins, plant; D008353:Mannitol; D008315:Malondialdehyde; C030737:indoleacetic acid; D000040:Abscisic Acid; C023623:brassinolide
Brassinosteroids; Dehydrin proteins; Hormonal balance; Plant growth; Triticum aestivum L.; Water deficit