Chicken IL-7 as a potent adjuvant enhances IBDV VP2 DNA vaccine immunogenicity and protective efficacy.
Our previous work has demonstrated that the mammalian interleukin-7 (IL-7) gene can enhance the immunogenicity of DNA vaccine. Whether chicken IL-7 (chIL-7) possesses the ability to enhance the immunogenicity of VP2 DNA vaccine of infectious bursal disease virus (IBDV) remained unknown. To investigate this, we constructed a VP2 antigenic region (VP2366) gene and chIL-7 gene vectors, co-immunized chicken with these vectors and analyzed the effects of the chIL-7 gene on VP2366 gene immunogenicity. Results showed that co-administrated chIL-7 gene with VP2 DNA vaccine significantly increased specific serum antibody titers against IBDV, and enhanced lymphocyte proliferation and IFN-γ and IL-4 productions. More importantly, chIL-7 gene significantly increased VP2366 gene-induced protection against virulent IBDV infection, indicating that the chIL-7 gene possessed the capacity to enhance VP2366 DNA vaccine immunogenicity, and therefore might function as a novel adjuvant for IBDV VP2 DNA vaccine. Mechanically, chIL-7 could stimulate the common cytokine receptor γ chain (γc) expressions in vitro and in vivo, which might be involved in chIL-7 enhancement of the immunogenicity of VP2 DNA vaccine.
Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071000, China.
Shanshan
S
Huo
Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071000, China.
Yuzhu
Y
Zuo
Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China.
Nan
N
Li
Department of Biotechnology, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
Xiujin
X
Li
Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China; Department of Dermatology, PLA Army General Hospital General Hospital, Beijing 100700, China.
Yonghong
Y
Zhang
Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China.
Liyue
L
Wang
Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071000, China; Rinpu (Baoding) Biological Pharmaceutical Co., LTD, Baoding 071004, China.
Hao
H
Liu
Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071000, China.
Jianlou
J
Zhang
Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China.
Dan
D
Cui
Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071000, China; Rinpu (Baoding) Biological Pharmaceutical Co., LTD, Baoding 071004, China.
Pingyou
P
He
Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China; Department of Biotechnology, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
Jian
J
Xu
Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071000, China.
Yan
Y
Li
Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071000, China; Rinpu (Baoding) Biological Pharmaceutical Co., LTD, Baoding 071004, China. Electronic address: zhuxiutong@163.com.
Xiutong
X
Zhu
Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071000, China. Electronic address: zhongf@hebau.edu.cn.
Fei
F
Zhong
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D002645:Chickens / Q000276:immunology*; D007114:Immunization; D000071497:Immunogenicity, Vaccine; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D015851:Interleukin-7 / Q000008:administration & dosage*; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D019444:Vaccines, DNA / Q000008:administration & dosage*; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology
D000276:Adjuvants, Immunologic; D015851:Interleukin-7; C082513:VP2 protein, infectious bursal disease virus; D019444:Vaccines, DNA; D015678:Viral Structural Proteins; D015847:Interleukin-4; D007371:Interferon-gamma
Bicistronic vector; Chicken interleuckin-7; Immunogenicity enhancement; Infectious bursal disease virus; VP2 DNA vaccine