miR-214 inhibits invasion and migration via downregulating GALNT7 in esophageal squamous cell cancer.
Previous studies verified that miR-214 is of great significance in the invasion and migration of a variety of cancers. It has been demonstrated that UDP-N-acetyl-α-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7(GALNT7) is a putative target of miR-214. We performed this study to figure out how miR-214 and GALNT7 play their roles in the invasion and migration of esophageal squamous cell carcinoma (ESCC). The expression of miR-214 was significantly downregulated in tumors compared to the corresponding non-tumor tissues while GALNT7 showed an opposite tendency. The low expression of miR-214 and the high expression of GALNT7 were found positively correlated with poor tumor differentiation (P = 0.004), tumor invasion (P = 0.013), and lymph node metastasis (P = 0.012) in ESCC patients. Functional study demonstrated that overexpression of miR-214 or knockdown of GALNT7 could weaken invasive and migratory ability in Eca109, TE1, and KYSE150. Moreover, tumorigenicity assay showed us mice injected with cells containing miR-214 mimic or GALNT7 small interfering RNA formed substantially smaller tumors than that in miR-214 inhibitor group. Consequently, we concluded that miR-214 shows potential to be a diagnostic marker and therapeutic target in ESCC.
Department of Thoracic Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, People's Republic of China.
Qijue
Q
Lu
Department of Cardiothoracic Surgery, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, People's Republic of China.
Li
L
Xu
Department of Thoracic Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, People's Republic of China.
Chunguang
C
Li
Department of Thoracic Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, People's Republic of China.
Yang
Y
Yuan
Department of Thoracic Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, People's Republic of China.
Shengdong
S
Huang
Department of Thoracic Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, People's Republic of China. drchenhz@sina.com.|Department of Thoracic Surgery, Changhai Hospital, The Second Military Medical University, 168# Changhai Road, Yangpu District, Shanghai, 200433, People's Republic of China. drchenhz@sina.com.
Hezhong
H
Chen
D000368:Aged; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D017350:N-Acetylgalactosaminyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays; D000097763:Polypeptide N-acetylgalactosaminyltransferase
D035683:MicroRNAs; D017350:N-Acetylgalactosaminyltransferases; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C547397:MIRN214 microRNA, human
ESCC; GALNT7; Invasion; Migration; miR-214