Effects of allergic diseases and age on the composition of serum IgG glycome in children.
It is speculated that immunoglobulin G (IgG) plays a regulatory role in allergic reactions. The glycans on the Fc region are known to affect IgG effector functions, thereby possibly having a role in IgG modulation of allergic response. This is the first study investigating patients' IgG glycosylation profile in allergic diseases. Subclass specific IgG glycosylation profile was analyzed in two cohorts of allergen sensitized and non-sensitized 3- to 11-year-old children (conducted at University of Aberdeen, UK and Children's Hospital Srebrnjak, Zagreb, Croatia) with 893 subjects in total. IgG was isolated from serum/plasma by affinity chromatography on Protein G. IgG tryptic glycopeptides were analyzed by liquid chromatography electrospray ionization mass spectrometry. In the Zagreb cohort IgG glycome composition changed with age across all IgG subclasses. In both cohorts, IgG glycome composition did not differ in allergen sensitized subjects, nor children sensitized to individual allergens, single allergen mean wheal diameter or positive wheal sum values. In the Zagreb study the results were also replicated for high total serum IgE and in children with self-reported manifest allergic disease. In conclusion, our findings demonstrate no association between serum IgG glycome composition and allergic diseases in children.
Genos Glycoscience Research Laboratory, Zagreb, Croatia.
Marija
M
Pezer
Genos Glycoscience Research Laboratory, Zagreb, Croatia.
Jerko
J
Stambuk
Children's Hospital Srebrnjak, Zagreb, Croatia.
Marija
M
Perica
University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia.
Genadij
G
Razdorov
Children's Hospital Srebrnjak, Zagreb, Croatia.
Ivana
I
Banic
Genos Glycoscience Research Laboratory, Zagreb, Croatia.
Frano
F
Vuckovic
Children's Hospital Srebrnjak, Zagreb, Croatia.
Adrijana Miletic
AM
Gospic
Genos Glycoscience Research Laboratory, Zagreb, Croatia.
Ivo
I
Ugrina
Children's Hospital Srebrnjak, Zagreb, Croatia.
Ana
A
Vecenaj
Genos Glycoscience Research Laboratory, Zagreb, Croatia.
Maja Pucic
MP
Bakovic
Children's Hospital Srebrnjak, Zagreb, Croatia.
Sandra Bulat
SB
Lokas
Children's Hospital Srebrnjak, Zagreb, Croatia.
Jelena
J
Zivkovic
Children's Hospital Srebrnjak, Zagreb, Croatia.|University of Osijek, Faculty of Medicine, Osijek, Croatia.
Davor
D
Plavec
Child Health, University of Aberdeen, Aberdeen, UK.
Graham
G
Devereux
Children's Hospital Srebrnjak, Zagreb, Croatia.|University of Osijek, Faculty of Medicine, Osijek, Croatia.
Mirjana
M
Turkalj
Genos Glycoscience Research Laboratory, Zagreb, Croatia.|University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia.
Gordan
G
Lauc
D000367:Age Factors; D002240:Carbohydrate Sequence; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D054794:Glycomics; D006031:Glycosylation; D006801:Humans; D006967:Hypersensitivity / Q000097:blood* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D011499:Protein Processing, Post-Translational; D006023:Glycoproteins
D007074:Immunoglobulin G; C046179:glycosylated IgG; D006023:Glycoproteins