Membrane-associated GRP78 helps subgroup J avian leucosis virus enter cells.
We previously identified chicken Annexin A2 (chANXA2) as a novel receptor for retrovirus avian leucosis virus subgroup J (ALV-J), using a DF1 cell line expressing the viral envelope (env) protein. To further probe whether other proteins participate in virus infection, we investigated several host proteins from co-immunoprecipitation with the DF1 cell line expressing viral env. Mass spectrometry analysis indicates that the chicken glucose-regulation protein 78 (chGRP78) of the DF1 membrane interacted with the ALV-J env protein. The results revealed that antibodies or siRNA to chGRP78 significantly inhibited ALV-J infection and replication, and over-expression of chGRP78 enabled the entry of ALV-J into non-susceptible cells. Taken together, these results are the first to report that chGRP78 functions to help ALV-J enter cells.
Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.|Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, China.
Lin
L
Wang
Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.|Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, China.|National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, China.
Mei
M
Mei
Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China. aijian@yzu.edu.cn.|Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, China. aijian@yzu.edu.cn.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, China. aijian@yzu.edu.cn.|Jiangsu Key Lab of Zoonosis, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, China. aijian@yzu.edu.cn.
Aijian
A
Qin
Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.|Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, China.
Jianqiang
J
Ye
Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.|Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, China.
Kun
K
Qian
Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.|Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, 225009, China.
Hongxia
H
Shao
D000818:Animals; D001353:Avian Leukosis / Q000821:virology*; D001354:Avian Leukosis Virus / Q000502:physiology*; D015153:Blotting, Western / Q000662:veterinary; D002460:Cell Line; D002645:Chickens / Q000821:virology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D047468:Immunoprecipitation / Q000662:veterinary; D013058:Mass Spectrometry / Q000662:veterinary; D008565:Membrane Proteins / Q000378:metabolism*; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D014759:Viral Envelope Proteins; C018551:glucose-regulated proteins