"What makes some rats live so long?" The mitochondrial contribution to longevity through balance of mitochondrial dynamics and mtDNA content.
Extremely interesting for aging research are those individuals able to reach older ages still with functions similar to those of younger counterparts. We examined liver samples from ad libitum-fed old (28-month-old, AL-28) and ad libitum-fed very old (32-month-old, AL-32) rats for a number of markers, relevant for mitochondrial functionality and mitochondrial DNA (mtDNA) content. As for the mtDNA content and the protein amounts of the citrate synthase and the antioxidant peroxiredoxin III there were no significant changes in the AL-32 animals. No significant longevity-related change was found for TFAM amount, but a 50% reduction in the amount of the Lon protease, responsible for turnover of TFAM inside mitochondria, characterized the AL-32 rats. No longevity-related change was observed also for the amounts of the mtDNA repair enzymes OGG1 and APE1, whereas the intra-mitochondrial amount of the cytochrome c protein showed a 50% increase in the AL-32 rats, indicating a likely reduced initiation of the intrinsic apoptotic pathway. Totally unexpected was the doubling of two proteins, very relevant for mitochondrial dynamics, namely MFN2 and DRP1, in the AL-32 rats. This prompted us to the calculation of all individual fusion indexes that grouped together in the AL-32 rats, while in the AL-28 animals were very different. We found a strong positive correlation between the fusion indexes and the respective mtDNA contents in two AL-28 and four AL-32 rats. This supports the idea that the limited prevalence of fusion above a still active fission should have ensured a functional mitochondrial network and should have led to a quite narrow range of high mtDNA contents, likely the best-suitable for extended longevity. Our findings strongly suggest that, among the multiple causes leading to the longevity of the AL-32 rats, the maintenance of an adult-like balance of mitochondrial dynamics seems to be very relevant for the regulation of mtDNA content and functionality.
Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona, 4, 70125 Bari, Italy.
Anna
A
Picca
Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona, 4, 70125 Bari, Italy.
Vito
V
Pesce
Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona, 4, 70125 Bari, Italy.
Giuseppe
G
Sirago
Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona, 4, 70125 Bari, Italy.
Flavio
F
Fracasso
Department of Aging and Geriatric Research, Institute on Aging, Division of Biology of Aging, University of Florida, 2004 Mowry Rd, Gainesville, FL 32611, (USA).
Christiaan
C
Leeuwenburgh
Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona, 4, 70125 Bari, Italy. Electronic address: angelamariaserena.lezza@uniba.it.
Angela Maria Serena
AMS
Lezza
D000375:Aging / Q000502:physiology*; D000818:Animals; D004260:DNA Repair; D004272:DNA, Mitochondrial / Q000235:genetics*; D008136:Longevity; D008297:Male; D008928:Mitochondria / Q000235:genetics*; D063154:Mitochondrial Dynamics; D051381:Rats; D011916:Rats, Inbred F344; D014157:Transcription Factors / Q000235:genetics
D004272:DNA, Mitochondrial; D014157:Transcription Factors
Aged liver mitochondria; Fusion index correlation with mtDNA content; Long-living rats; Mitochondrial dynamics; mtDNA and related proteins