Nitrogen regulates CRY1 phosphorylation and circadian clock input pathways.
The delayed flowering phenotype caused by nitrogen (N) fertilizer application has been known for a long time, but we know little about the specific molecular mechanism for this phenomenon before. Our study indicated that low nitrogen increases the NADPH/NADP(+) and ATP/AMP ratios which affect adenosine monophosphate-activated protein kinase (AMPK) activity and phosphorylation and abundance of nuclear CRY1 protein. Then CRY1 acts in the N signal input pathway to the circadian clock. Here we further discuss: (1) the role of C/N ratio in flowering, (2) circadian oscillation of plant AMPK transcripts and proteins, (3) conservation of nutrition-mediated CRY1 phosphorylation and degradation, and (4) crosstalks between nitrogen signals and nitric oxide (NO) signals in flowering.
a College of Resources Science and Technology , Sichuan Agricultural University , Chengdu , China.
Yang-Hong
YH
Zhou
a College of Resources Science and Technology , Sichuan Agricultural University , Chengdu , China.
Zhong-Wei
ZW
Zhang
b College of Life Science , Capital Normal University , Beijing , China.
Chong
C
Zheng
a College of Resources Science and Technology , Sichuan Agricultural University , Chengdu , China.|b College of Life Science , Capital Normal University , Beijing , China.
Shu
S
Yuan
b College of Life Science , Capital Normal University , Beijing , China.
Yikun
Y
He
D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D057906:Circadian Clocks / Q000235:genetics / Q000502:physiology; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology; D056931:Cryptochromes / Q000235:genetics / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D050901:Nitrate Reductase / Q000235:genetics / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D010766:Phosphorylation
D029681:Arabidopsis Proteins; C538962:CRY1 protein, Arabidopsis; D056931:Cryptochromes; D000249:Adenosine Monophosphate; D009249:NADP; D000255:Adenosine Triphosphate; D050901:Nitrate Reductase; D055372:AMP-Activated Protein Kinases; D009584:Nitrogen
Adenosine monophosphate-activated protein kinase; circadian clock; cryptochrome 1; nitrate reductase; nitrogen-regulated flowering