Statistical experimental designs for the production of secondary metabolites in plant cell suspension cultures.
Statistical experimental designs, also known as the "design of experiments" (DoE) approach, are widely used to improve not only technical processes but also to answer questions in the agricultural, medical and social sciences. Although many articles have been published about the application of DoE in these fields, few studies have addressed the use of DoE in the plant sciences, particularly in the context of plant cell suspension cultures (PCSCs). Compounds derived from PCSCs can be developed as pharmaceuticals, chemical feedstocks and cosmetic ingredients, and statistical experimental designs can be used to improve the productivity of the cells and the yield and/or quality of the target compounds in a cost efficient manner. In this article, we summarize recent findings concerning the application of statistical approaches to improve the performance of PCSCs and discuss the potential future applications of this approach.
Aachen-Maastricht Institute for Biobased Materials, Maastricht University, P.O. Box 616, 6200MD, Maastricht, The Netherlands.
Christian
C
Schmitz
Aachen-Maastricht Institute for Biobased Materials, Maastricht University, P.O. Box 616, 6200MD, Maastricht, The Netherlands.
Leonie
L
Fritsch
Aachen-Maastricht Institute for Biobased Materials, Maastricht University, P.O. Box 616, 6200MD, Maastricht, The Netherlands.|Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6, 52074, Aachen, Germany.|Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany.
Rainer
R
Fischer
Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6, 52074, Aachen, Germany. stefan.schillberg@ime.fraunhofer.de.|Institute for Phytopathology and Applied Zoology, Phytopathology Department, Justus-Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany. stefan.schillberg@ime.fraunhofer.de.
Stefan
http://orcid.org/0000-0002-1896-4575
S
Schillberg
Aachen-Maastricht Institute for Biobased Materials, Maastricht University, P.O. Box 616, 6200MD, Maastricht, The Netherlands.|Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstraße 6, 52074, Aachen, Germany.
Stefan
S
Rasche
D018929:Cell Culture Techniques / Q000379:methods*; D003627:Data Interpretation, Statistical; D059828:Plant Cells / Q000378:metabolism*; D012107:Research Design
Design of experiments (DoE); Factorial design; One-factor-at-a-time (OFAT); Plant cell cultivation; Process optimization