A comparative proteomic analysis of Salmonella typhimurium under the regulation of the RstA/RstB and PhoP/PhoQ systems.
In pathogenic bacteria, the two-component regulatory systems (TCSs) play important roles in signal transduction and regulation of their pathogenesis. Here, we used quantitative proteomic methods to comparatively analyze functional networks under the control of the RstA/RstB system versus the PhoP/PhoQ system in Salmonella typhimurium. By comparing the proteomic profile from a wild-type strain to that from a ΔrstB strain or a ΔphoPQ strain under a condition known to activate these TCSs, we found that the levels of 159 proteins representing 6.92% of the 2297 proteins identified from the ΔrstB strain and 341 proteins representing 14.9% of the 2288 proteins identified from the ΔphoPQ strain were significantly changed, respectively. Bioinformatics analysis revealed that the RstA/RstB system and the PhoP/PhoQ system coordinated with regard to the regulation of specific proteins as well as metabolic processes. Our observations suggested that the regulatory networks controlled by the PhoP/PhoQ system were much more extensive than those by the RstA/RstB system, whereas the RstA/RstB system specifically regulated expression of the constituents participating in pyrimidine metabolism and iron acquisition. Additional results also suggested that the RstA/RstB system was required for regulation of Salmonella motility and invasion.
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China; Hung Vuong University, Phu Tho, Vietnam.
Trung-Kien
TK
Tran
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
Qiang-Qiang
QQ
Han
The School of Life Sciences, Arizona State University, Tempe, AZ 85287-4501, USA; The Center for Infectious Diseases and Vaccinology at the Biodesign Institute, Arizona State University, Tempe, AZ 85287-4501, USA. Electronic address: Yixin.Shi@asu.edu.
Yixin
Y
Shi
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China. Electronic address: guol@whu.edu.cn.
Lin
L
Guo
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D019295:Computational Biology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D007501:Iron / Q000378:metabolism; D009154:Mutation; D040901:Proteomics; D011743:Pyrimidines / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence
D001426:Bacterial Proteins; C079792:PhoQ protein, Bacteria; D011743:Pyrimidines; C061064:PhoP protein, Bacteria; D007501:Iron
Coordinated regulation; PhoP/PhoQ system; Proteomics; RstA/RstB system; Salmonella typhimurium