Predictive modeling of microbial single cells: A review.
In practice, food products tend to be contaminated with food-borne pathogens at a low inoculum level. However, the huge potential risk cannot be ignored because microbes may initiate high-speed growth suitable conditions during the food chain, such as transportation or storage. Thus, it is important to perform predictive modeling of microbial single cells. Several key aspects of microbial single-cell modeling are covered in this review. First, based on previous studies, the techniques of microbial single-cell data acquisition and growth data collection are presented in detail. In addition, the sources of microbial single-cell variability are also summarized. Due to model microbial growth, traditional deterministic mathematical models have been developed. However, most models fail to make accurate predictions at low cell numbers or at the single-cell level due to high cell-to-cell heterogeneity. Stochastic models have been a subject of great interest; and these models take into consideration the variability in microbial single-cell behavior.
a Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing , Zhejiang University , Hangzhou , Zhejiang , China.
Tian
T
Ding
a Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing , Zhejiang University , Hangzhou , Zhejiang , China.
Xin-Yu
XY
Liao
b Institute of Food Quality and Safety, University of Shanghai for Science and Technology , Shanghai , China.
Qing-Li
QL
Dong
a Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing , Zhejiang University , Hangzhou , Zhejiang , China.
Xiao-Ting
XT
Xuan
a Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing , Zhejiang University , Hangzhou , Zhejiang , China.
Shi-Guo
SG
Chen
a Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing , Zhejiang University , Hangzhou , Zhejiang , China.
Xing-Qian
XQ
Ye
a Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing , Zhejiang University , Hangzhou , Zhejiang , China.
Dong-Hong
DH
Liu
D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000254:growth & development*; D008954:Models, Biological; D059010:Single-Cell Analysis
Single cell; food; model; predictive microbiology; variability