The effects of femtosecond laser-textured Ti-6Al-4V on wettability and cell response.
To study the biological activity effects of femtosecond laser-induced structures on cell behavior, TA6V samples were micro-textured with focused femtosecond laser pulses generating grooves of various dimensions on the micrometer scale (width: 25-75μm; depth: 1-10μm). LIPSS (Laser Induced Periodic Surface Structures) were also generated during the laser irradiation, providing a supplementary structure (sinusoidal form) of hundreds of nanometers at the bottom of the grooves oriented perpendicular (⊥ LIPPS) or parallel (// LIPPS) to the direction of these grooves. C3H10 T1/2 murine mesenchymal stem cells were cultivated on the textured biomaterials. To have a preliminary idea of the spreading of biological media on the substrate, prior to cell culture, contact angle measurement were performed. This showed that the post-irradiation hydrophilicity of the samples can decrease with time according to its storage environment. The multiscale structuration either induced a collaborative or a competitive influence of the LIPSS and grooves on the cells. It has been shown that cells individually and collectively were most sensitive to microscale grooves which were narrower than 25μm and deeper than 5μm with ⊥ LIPPS. In some cases, cells were individually sensitive to the LIPSS but the cell layer organization did not exhibit significant differences in comparison to a non-textured surface. These results showed that cells are more sensitive to the nanoscale structures (LIPSS), unless the microstructures's size is close to the cell size and deeper than 5μm. There, the cells are sensitive to the microscale structures and go on spreading following these structures.
Université de Lyon, Ecole Centrale de Lyon, Laboratoire de Tribologie et Dynamique des Systèmes (UMR 5513 CNRS), 36 avenue Guy de Collongue, 69134 Ecully Cedex, France.
Ophélie
O
Raimbault
Université de Lyon, Ecole Centrale de Lyon, Laboratoire de Tribologie et Dynamique des Systèmes (UMR 5513 CNRS), 36 avenue Guy de Collongue, 69134 Ecully Cedex, France. Electronic address: stephane.benayoun@ec-lyon.fr.
Stephane
S
Benayoun
Institut de Science des Matériaux de Mulhouse (IS2M), CNRS UMR7361, Université de Haute-Alsace, 15 rue Jean Starcky, Mulhouse, France.
Karine
K
Anselme
Université de Lyon, Université de Saint-Étienne, Laboratoire Hubert Curien (UMR 5516 CNRS), 18 rue du Professeur Benoit Lauras, 42000 Saint Etienne, France; GIE Manutech USD, 18 rue du Professeur Benoît Lauras, 42000 St Etienne, France.
Cyril
C
Mauclair
Institut de Science des Matériaux de Mulhouse (IS2M), CNRS UMR7361, Université de Haute-Alsace, 15 rue Jean Starcky, Mulhouse, France.
Tatiana
T
Bourgade
Department of Chemical Engineering, McGill University, 3610 University Street, Montreal, Quebec, H3A 0C5, Canada.
Anne-Marie
AM
Kietzig
Department of Chemical Engineering, McGill University, 3610 University Street, Montreal, Quebec, H3A 0C5, Canada.
Pierre-Luc
PL
Girard-Lauriault
Université de Lyon, Ecole Centrale de Lyon, Laboratoire de Tribologie et Dynamique des Systèmes (UMR 5513 CNRS), 36 avenue Guy de Collongue, 69134 Ecully Cedex, France; GIE Manutech USD, 18 rue du Professeur Benoît Lauras, 42000 St Etienne, France.
Stephane
S
Valette
Université de Lyon, Université de Saint-Étienne, Laboratoire Hubert Curien (UMR 5516 CNRS), 18 rue du Professeur Benoit Lauras, 42000 Saint Etienne, France; GIE Manutech USD, 18 rue du Professeur Benoît Lauras, 42000 St Etienne, France.
Christophe
C
Donnet
D000497:Alloys; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D007834:Lasers; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*; D017155:Wettability
D000497:Alloys; D001672:Biocompatible Materials; C031462:titanium alloy (TiAl6V4); D014025:Titanium
Cell adhesion; Cell spreading; Contact guidance; Femtosecond laser; Multiscale texturing; Static contact angle