In vitro evaluation of actively targetable superparamagnetic nanoparticles to the folate receptor positive cancer cells.
Engineering of a physiologically compatible, stable and targetable SPIONs-CA-FA formulation was reported. Initially fabricated superparamagnetic iron oxide nanoparticles (SPIONs) were coated with citric acid (CA) to hamper agglomeration as well as to ameliorate biocompatibility. Folic acid (FA) as a targeting agent was then conjugated to the citric acid coated SPIONs (SPIONs-CA) for targeting the specific receptors expressed on the FAR+ cancer cells. Physiochemical characterizations were then performed to assure required properties like stability, size, phase purity, surface morphology, chemical integrity and magnetic properties. In vitro evaluations (MTT assay) were performed on HeLa, HSF 1184, MDA-MB-468 and MDA-MB-231cell lines to ensure the biocompatibility of SPIONs-CA-FA. There were no morphological changes and lysis in contact with erythrocytes recorded for SPIONs-CA-FA and SPIONs-CA. High level of SPIONs-CA-FA binding to FAR+ cell lines was assured via qualitative and quantitative in vitro binding studies. Hence, SPIONs-CA-FA was introduced as a promising tool for biomedical applications like magnetic hyperthermia and drug delivery. The in vitro findings presented in this study need to be compared with those of in vivo studies.
Institute of Bioproduct Development, Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, Skudai, 81310 Johor Bahru, Johor, Malaysia.
Rozita
R
Nasiri
Institute of Bioproduct Development, Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, Skudai, 81310 Johor Bahru, Johor, Malaysia.
Javad
J
Hamzehalipour Almaki
Institute of Bioproduct Development, Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, Skudai, 81310 Johor Bahru, Johor, Malaysia. Electronic address: ani@cheme.utm.my.
Ani Binti
AB
Idris
Institute of Bioproduct Development, Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, Skudai, 81310 Johor Bahru, Johor, Malaysia; Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21300 Kuala Terengganu, Terengganu, Malaysia.
Fadzilah Adibah
FA
Abdul Majid
Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
Mahtab
M
Nasiri
Biology Research Centre, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
Mojtaba
M
Salouti
Institute of Bioproduct Development, Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, Skudai, 81310 Johor Bahru, Johor, Malaysia.
Muhammad
M
Irfan
Faculty of Biomedical Engineering, Universiti Teknologi Malaysia, Skudai, 81310 Johor Bahru, Johor, Malaysia.
Neda
N
Amini
Faculty of Biomedical Engineering, Universiti Teknologi Malaysia, Skudai, 81310 Johor Bahru, Johor, Malaysia.
Mohsen
M
Marvibaigi
D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001773:Blood Cells / Q000187:drug effects / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019343:Citric Acid / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry*; D058973:Folate Receptors, GPI-Anchored / Q000378:metabolism*; D005492:Folic Acid / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D003911:Dextrans; D058973:Folate Receptors, GPI-Anchored; D058185:Magnetite Nanoparticles; D019343:Citric Acid; D005492:Folic Acid; C065507:ferumoxides
Bioconjugation; Co-precipitation; FAR+ cancer cells; Iron oxide nanoparticles; Superparamagnetic; Targeting