The Developmental Regulator SEEDSTICK Controls Structural and Mechanical Properties of the Arabidopsis Seed Coat.
Although many transcription factors involved in cell wall morphogenesis have been identified and studied, it is still unknown how genetic and molecular regulation of cell wall biosynthesis is integrated into developmental programs. We demonstrate by molecular genetic studies that SEEDSTICK (STK), a transcription factor controlling ovule and seed integument identity, directly regulates PMEI6 and other genes involved in the biogenesis of the cellulose-pectin matrix of the cell wall. Based on atomic force microscopy, immunocytochemistry, and chemical analyses, we propose that structural modifications of the cell wall matrix in the stk mutant contribute to defects in mucilage release and seed germination under water-stress conditions. Our studies reveal a molecular network controlled by STK that regulates cell wall properties of the seed coat, demonstrating that developmental regulators controlling organ identity also coordinate specific aspects of cell wall characteristics.
Dipartimento di BioScienze, Università degli Studi di Milano, 20133 Milan, Italy.|Consiglio Nazionale delle Ricerche, Istituto di Biofisica, 20133 Milan, Italy.
Ignacio
0000-0003-1886-0095
I
Ezquer
Dipartimento di BioScienze, Università degli Studi di Milano, 20133 Milan, Italy.
Chiara
0000-0003-3644-2890
C
Mizzotti
Laboratoire Glycobiologie et Matrice Extracellulaire, Normandie Université, UNIROUEN, Végétal, Agronomie, Sol, et Innovation (VASI), 76821 Mont-Saint-Aignan, France.|Centre Mondial de l'Innovation-Laboratoire de Nutrition Végétale, 35400 Saint Malo, France.
Eric
0000-0002-0292-9273
E
Nguema-Ona
Laboratoire Glycobiologie et Matrice Extracellulaire, Normandie Université, UNIROUEN, Végétal, Agronomie, Sol, et Innovation (VASI), 76821 Mont-Saint-Aignan, France.
Maxime
0000-0001-7192-0945
M
Gotté
Laboratoire de Reproduction et Développement des Plantes, INRA, CNRS, ENS, UCB Lyon 1, Université de Lyon, 69364 Lyon Cedex 07, France.
Léna
L
Beauzamy
Plant Genomics and Breeding Center, Technology Development Center, Federal University of Pelotas, RS 96010-900, Brazil.
Vivian Ebeling
0000-0002-8326-7549
VE
Viana
Laboratoire de Reproduction et Développement des Plantes, INRA, CNRS, ENS, UCB Lyon 1, Université de Lyon, 69364 Lyon Cedex 07, France.
Nelly
0000-0002-4869-2451
N
Dubrulle
Plant Genomics and Breeding Center, Technology Development Center, Federal University of Pelotas, RS 96010-900, Brazil.
Antonio
0000-0001-8835-8071
A
Costa de Oliveira
Dipartimento di BioScienze, Università degli Studi di Milano, 20133 Milan, Italy.
Elisabetta
E
Caporali
Laboratoire Glycobiologie et Matrice Extracellulaire, Normandie Université, UNIROUEN, Végétal, Agronomie, Sol, et Innovation (VASI), 76821 Mont-Saint-Aignan, France.
Abdoul-Salam
AS
Koroney
Laboratoire de Reproduction et Développement des Plantes, INRA, CNRS, ENS, UCB Lyon 1, Université de Lyon, 69364 Lyon Cedex 07, France.
Arezki
0000-0002-2780-4717
A
Boudaoud
Laboratoire Glycobiologie et Matrice Extracellulaire, Normandie Université, UNIROUEN, Végétal, Agronomie, Sol, et Innovation (VASI), 76821 Mont-Saint-Aignan, France.
Azeddine
0000-0001-7032-6047
A
Driouich
Dipartimento di BioScienze, Università degli Studi di Milano, 20133 Milan, Italy lucia.colombo@unimi.it.|Consiglio Nazionale delle Ricerche, Istituto di Biofisica, 20133 Milan, Italy.
Lucia
0000-0001-8415-1399
L
Colombo
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D026161:MADS Domain Proteins; C000622995:PMEI9 protein, Arabidopsis; C476012:SEEDSTICK protein, Arabidopsis; D014157:Transcription Factors