The mimic epitopes of Mycobacterium tuberculosis screened by phage display peptide library have serodiagnostic potential for tuberculosis.
Mycobacterium tuberculosis is an obligate pathogenic bacterial species in the family of Mycobacteriaceae and attracts excessive immune responses which cause pathology of the lungs in active tuberculosis. The lack of more sensitive and effective diagnosis reagents advocates a further recognition for the fast diagnostic and immunological measures for tuberculosis. Here, two 12-mer peptides with core sequences of SVSVGMKPSPRP (CS1) and TMGFTAPRFPHY (CS2) were screened from a phage display random peptide library using the purified mixed tuberculosis-positive serum as a target. Enzyme-linked immunosorbent assay (ELISA) and dot immunobinding assay verified that positive phages exhibited strong binding affinity to mixed tuberculosis-positive serum. BLAST analysis showed that the two sequences may be mimotopes of the Mycobacterium tuberculosis The diagnostic potential for two synthetic mimotope peptides CS1 and CS2 was evaluated using different panels of serum samples (n = 181) by ELISA, and the diagnostic parameters were calculated. CS1 and CS2 achieved sensitivity of 89.41% and 85.88%, and specificities were 90.63% and 87.50%, respectively. We hypothesized that the diagnostic based on CS1 and CS2 may become a promising strategy to enhance the detection of Mycobacterium tuberculosis infection due to higher specificity and sensitivity. Therefore, CS1 and CS2 may possess potentials to provide an experimental basis for the diagnosis of tuberculosis.
Institute of Pathogenic Biology, Medical College, University of South China; Hunan Provincial Key Laboratory for Basic Medicine; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang 421001, China.
Li
L
Wang
Institute of Pathogenic Biology, Medical College, University of South China; Hunan Provincial Key Laboratory for Basic Medicine; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang 421001, China.
Xiangying
X
Deng
State Key Laboratory for Infectious Disease Prevention and Control/National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Haican
H
Liu
Institute of Pathogenic Biology, Medical College, University of South China; Hunan Provincial Key Laboratory for Basic Medicine; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang 421001, China.
Lanhua
L
Zhao
Institute of Pathogenic Biology, Medical College, University of South China; Hunan Provincial Key Laboratory for Basic Medicine; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang 421001, China.
Xiaolong
X
You
Institute of Pathogenic Biology, Medical College, University of South China; Hunan Provincial Key Laboratory for Basic Medicine; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang 421001, China.
Pei
P
Dai
State Key Laboratory for Infectious Disease Prevention and Control/National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Kanglin
K
Wan
Institute of Pathogenic Biology, Medical College, University of South China; Hunan Provincial Key Laboratory for Basic Medicine; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang 421001, China zengyihua21cn@126.com.
Yanhua
Y
Zeng
D061505:Cell Surface Display Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D019151:Peptide Library; D012372:ROC Curve; D012698:Serologic Tests; D014376:Tuberculosis / Q000175:diagnosis* / Q000276:immunology*
D000939:Epitopes; D019151:Peptide Library
Mycobacterium tuberculosis; mimic epitope; phage display peptide library; serodiagnosis