Increased Intestinal Absorption of Genistein by Coadministering Verapamil in Rats.
Combination of genistein (GT) and verapamil, a P-glycoprotein (P-gp) inhibitor, can increase GT absorption in situ perfusion technology in rat. To date, little information is yet available about the effect of verapamil on oral absorption of GT in vivo. In this study, a simple and reproducible HPLC-UV method was developed and validated for determination of total GT in rat plasma. Based on this, a pharmacokinetic experiment was designed to characterize biopharmaceutical properties of GT with or without coadministration of verapamil (10.0, 20.0, 30.0 mg/kg) in rats. The coadministration of verapamil (30.0 mg/kg) with GT caused a significant increase of the maximum GT plasma concentration (1.31-fold vs. GT, P < 0.05) and area under the curve (1.39-fold vs. GT, P < 0.05). Our data show that verapamil would increase intestinal absorption of GT in rat, suggesting there is some drug-nutrition interaction between verapamil and GT.
School of Pharmaceutical Science, Nanchang University, No 461, bayi Ave., Nanchang, 330006, Republic of China. xiebaogang@ncu.edu.cn.
Baogang
B
Xie
School of Pharmaceutical Science, Nanchang University, No 461, bayi Ave., Nanchang, 330006, Republic of China.
Huiyun
H
Wang
School of Pharmaceutical Science, Nanchang University, No 461, bayi Ave., Nanchang, 330006, Republic of China.
Huiqin
H
Zou
School of Pharmaceutical Science, Nanchang University, No 461, bayi Ave., Nanchang, 330006, Republic of China.
Yalan
Y
Liu
Nanchang University Archives, Nanchang, 330047, People's Republic of China.
Xiangyu
X
Kong
School of Science, Nanchang University, Nanchang, 330006, People's Republic of China.
Xiuzhong
X
Fang
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors; D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004347:Drug Interactions / Q000502:physiology; D019833:Genistein / Q000008:administration & dosage*; D007408:Intestinal Absorption / Q000187:drug effects*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014700:Verapamil / Q000008:administration & dosage*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D014700:Verapamil; D019833:Genistein
Genistein; Oral absorption; P-gp; Pharmacokinetics; Verapamil