Role of miRNAs in Epicardial Adipose Tissue in CAD Patients with T2DM.
Background. Epicardial adipose tissue (EAT) is identified as an atypical fat depot surrounding the heart with a putative role in the involvement of metabolic disorders, including obesity, type-2 diabetes mellitus, and atherosclerosis. We profiled miRNAs in EAT of metabolic patients with coronary artery disease (CAD) and type-2 diabetes mellitus (T2DM) versus metabolically healthy patients by microarray. Compared to metabolically healthy patients, we identified forty-two miRNAs that are differentially expressed in patients with CAD and T2DM from Xinjiang, China. Eleven miRNAs were selected as potential novel miRNAs according to P value and fold change. Then the potential novel miRNAs targeted genes were predicted via TargetScan, PicTar, and miRTarbase, and the function of the target genes was predicted via Gene Ontology (GO) analysis while the enriched KEGG pathway analyses of the miRNAs targeted genes were performed by bioinformatics software DAVID. Then protein-protein interaction networks of the targeted gene were conducted by online software STRING. Finally, using microarray, bioinformatics approaches revealed the possible molecular mechanisms pathogenesis of CAD and T2DM. A total of 11 differentially expressed miRNAs were identified and among them, hsa-miR-4687-3p drew specific attention. Bioinformatics analysis revealed that insulin signaling pathway is the central way involved in the progression of metabolic disorders. Conclusions. The current findings support the fact that miRNAs are involved in the pathogenesis of metabolic disorders in EAT of CAD patients with T2DM, and validation of the results of these miRNAs by independent and prospective study is certainly warranted.
Medicine Department, Shihezi University, Shihezi, Xinjiang 832000, China.
Yang
0000-0001-5340-3944
Y
Liu
Department of Cardiology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830000, China.
Wenbo
0000-0001-8900-7871
W
Fu
Medicine Department, Shihezi University, Shihezi, Xinjiang 832000, China.
Mu
M
Lu
Medicine Department, Shihezi University, Shihezi, Xinjiang 832000, China.
Shitao
S
Huai
Medicine Department, Shihezi University, Shihezi, Xinjiang 832000, China.
Yaqin
Y
Song
Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Shihezi University School of Medicine, Shihezi, Xinjiang 832008, China.
Yutao
0000-0001-8738-0488
Y
Wei
D000273:Adipose Tissue / Q000378:metabolism*; D000368:Aged; D002681:China; D016000:Cluster Analysis; D019295:Computational Biology; D003324:Coronary Artery Disease / Q000150:complications / Q000208:ethnology / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000208:ethnology / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007328:Insulin / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010496:Pericardium / Q000378:metabolism*; D025941:Protein Interaction Mapping; D015398:Signal Transduction; D012984:Software
D007328:Insulin; D035683:MicroRNAs