Colorimetric aptasensor for progesterone detection based on surfactant-induced aggregation of gold nanoparticles.
This paper proposes an aptasensor for progesterone (P4) detection in human serum and urine based on the aggregating behavior of gold nanoparticles (AuNPs) controlled by the interactions among P4-binding aptamer, target P4 and cationic surfactant hexadecyltrimethylammonium bromide (CTAB). The aptamer can form an aptamer-P4 complex with P4, leaving CTAB free to aggregate AuNPs in this aptasensor. Thus, the sensing solution will turn from red (520 nm) to blue (650 nm) in the presence of P4 because P4 aptamers are used up firstly owing to the formation of an aptamer-P4 complex, leaving CTAB free to aggregate AuNPs. However, in the absence of P4, CTAB combines with aptamers so that AuNPs still remain dispersed. Therefore, this assay makes it possible to detect P4 not only by absorbance measurement but also through naked eyes. By monitoring the variation of absorbance and color, a CTAB-induced colorimetric assay for P4 detection was established with a detection limit of 0.89 nM. Besides, the absorbance ratio A650/A520 has a linear correlation with the P4 concentration of 0.89-500 nM. Due to the excellent recoveries in serum and urine, this biosensor has great potential with respect to the visual and instrumental detection of P4 in biological fluids.
School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
Gaoshang
G
Du
School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China. Electronic address: zjuwlm@163.com.
Lumei
L
Wang
School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
Dongwei
D
Zhang
School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
Xuan
X
Ni
School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
Xiaotong
X
Zhou
School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
Hanyi
H
Xu
School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
Lurong
L
Xu
Shanghai Environmental Monitoring Center, Shanghai 200235, PR China.
Shijian
S
Wu
School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
Tong
T
Zhang
School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
Wenhao
W
Wang
D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D011374:Progesterone / Q000097:blood* / Q000652:urine*; D012680:Sensitivity and Specificity; D013501:Surface-Active Agents / Q000737:chemistry
D052157:Aptamers, Nucleotide; D002593:Cetrimonium Compounds; D013501:Surface-Active Agents; D011374:Progesterone; D006046:Gold; D000077286:Cetrimonium
Aptamer; AuNPs; Biosensor; Gonadal hormone; Rapid testing; Visual test