Gd@C82-(ethylenediamine)8Nanoparticle: A New High-Efficiency Water-Soluble ROS Scavenger.
It is important to maintain a reactive oxygen species (ROS) balance in organisms; thus, a valid ROS scavenger with good biocompatibility is urgently required. To prepare a high-efficiency ROS scavenger, multiple ethylenediamine (EDA) groups are bonded for the first time to a metallofullerene Gd@C82to obtain water-soluble Gd@C82-(EDA)8nanoparticles (NPs) through a facile solid-liquid reaction. Gd@C82-(EDA)8NPs with a relatively better conjugation possess an excellent capability to scavenge hydroxyl radicals. Moreover, Gd@C82-(EDA)8NPs exhibited a remarkable cytoprotective effect against H2O2-induced injuries to human epidermal keratinocytes-adult (HEK-a) cells at a low concentration of 2.5 μM. In contrast, Gd@C82-(OH)26NPs that modified with hydroxyls show an apparent protective effect at a much higher concentration of 40 μM. This outstanding cytoprotective performance of Gd@C82-(EDA)8NPs is mainly attributed to their extremely high cellular uptake and comparably strong conjugation. Gd@C82-(EDA)8NPs with good biocompatibility exhibit excellent ROS scavenging capability even at a significantly low concentration, which promotes its versatile applications in cosmetics and biomedicine.
Beijing National Laboratory for Molecular Sciences, Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Jie
J
Li
Beijing National Laboratory for Molecular Sciences, Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Mirong
M
Guan
Beijing National Laboratory for Molecular Sciences, Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
Taishan
T
Wang
Beijing National Laboratory for Molecular Sciences, Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Mingming
M
Zhen
Beijing National Laboratory for Molecular Sciences, Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Fuwen
F
Zhao
Beijing National Laboratory for Molecular Sciences, Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
Chunying
C
Shu
Beijing National Laboratory for Molecular Sciences, Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Chunru
C
Wang
D002460:Cell Line; D005029:Ethylenediamines; D037741:Fullerenes; D005682:Gadolinium; D006801:Humans; D006861:Hydrogen Peroxide; D053758:Nanoparticles; D017382:Reactive Oxygen Species; D014867:Water
D005029:Ethylenediamines; D037741:Fullerenes; D017382:Reactive Oxygen Species; D014867:Water; D005682:Gadolinium; D006861:Hydrogen Peroxide
Gd@C82-(EDA)8 nanoparticles; ROS scavenger; cellular uptake; conjugation; cytoprotective effect