[Effect of Amphotericin B on human nasal ciliary beat frequency].
OBJECTIVE
To investigate the effects of Amphotericin B on ciliary beat frequency (CBF) in in vitro cultured human nasal epithelial cells.


METHODS
Human nasal epithelial cells derived from uncinate process or inferior turbinate of six patients were cultured in vitro, and treated with different concentrations of Amphotericin B(0.25、0.5、1.0、2.0、4.0 μg/ml)over a peroid of 7~10 d; normal saline(NS)was used as a negative control. CBF was detected before treatment and recorded continuously for 20 min after treatment.


RESULTS
CBF of epithelial cells in normal saline (NS) control group showed no significant changes in 20 min(F=0.351, P>0.05); Amphotericin B at 0.25 μg/ml(F=0.286, P>0.05), 0.5 μg/ml(F=0.468, P>0.05) or 1.0 μg/ml(F=0.383, P>0.05) did not affect epithelial CBF in 20 min measurement period; Amphotericin B at 2.0 μg/ml decreased epithelial CBF with time(F=1.908, P<0.05), significant changes were observed after 7 min of drug treatment; Amphotericin B at 4.0 μg/ml decreased epithelial CBF with time(F=8.223, P<0.05), significant changes were observed after 6 min of drug treatment.


CONCLUSIONS
Amphotericin B caused a dose-dependent decrease of human nasal CBF. Therefore, the influence of this drug on ciliary function should be considered when nasal topically applied.
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otorhinolaryngology.
J
J
Jiao
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otorhinolaryngology; Department of allergy, Beijing Tongren Hospital, Capital Medical University.
L
L
Zhang
D000666:Amphotericin B / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002452:Cell Count; D002923:Cilia / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D009297:Nasal Mucosa / Q000166:cytology*; D013997:Time Factors; D014420:Turbinates / Q000166:cytology
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000666:Amphotericin B