Psychroflexus saliphilus sp. nov., isolated from a marine solar saltern.
A Gram-stain-negative, non-motile, rod-shaped, orange-pigmented strain, WDS4A13T, was isolated from a marine solar saltern in Weihai, China. Strain WDS4A13T grew under optimal conditions of pH 7.0-8.0, 33 °C and with 6 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain WDS4A13T belonged to the genus Psychroflexus. The levels of similarity between the 16S rRNA gene sequence of strain WDS4A13T and those of the type strains of recognized species of the genus Psychroflexus were 92.0-97.3 %. The polar lipid profile of the novel isolate consisted of two unidentified phospholipids, diphosphatidylglycerol, phosphatidylethanolamine, a glycolipid and an unidentified lipid as the major polar lipids. The predominant menaquinone was MK-6, and iso-C15 : 1 G, iso-C15 : 0 and C15 : 0 were the major fatty acids. The DNA G+C content was 35.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain WDS4A13T represents a novel species within the genus Psychroflexus, for which the name Psychroflexus saliphilus sp. nov. is proposed. The type strain is WDS4A13T (=MCCC 1H00134T=KCTC 52043T).
College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
Sang
S
Jin
College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
Jun
J
Xia
Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.
Christopher A
CA
Dunlap
Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.
Alejandro P
AP
Rooney
College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
Zong-Jun
ZJ
Du
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6