Novel Bovine Papillomavirus Type Discovered by Rolling-Circle Amplification Coupled with Next-Generation Sequencing.
Currently, fifteen bovine papillomavirus (BPV) types have been identified and classified into four genera: Deltapapillomavirus, Epsilonpapillomavirus, Dyoxipapillomavirus, and Xipapillomavirus. Here, the complete genome sequence of a new BPV type (BPV 04AC14) recovered from a papillomatous lesion is reported. The genome is 7,282 bp in length and exhibits the classic genetic organization and motifs of the members of Papillomaviridae. Maximum likelihood phylogenetic analyses revealed that BPV 04AC14 clusters with members of the Xipapillomavirus genus. The nucleotide sequence of the L1 capsid protein of the novel BPV is closely related to its counterpart, BPV3, with which it shares 79% similarity. These findings suggest that this virus is a new BPV type of the Xipapillomavirus genus.
Laboratório de Virologia - Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.|Centro de Ciências Biológicas e da Natureza, Universidade Federal do Acre Rio Branco, Acre, Brazil.
Flavio R C
FR
da Silva
Laboratório de Virologia - Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Samuel P
SP
Cibulski
Laboratório de Virologia - Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Cíntia
C
Daudt
Laboratório de Virologia - Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Matheus N
MN
Weber
Laboratório de Virologia - Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Lorena L B
LL
Guimarães
Universidade de Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil.
André F
AF
Streck
Laboratório de Biologia Molecular - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Fundação Estadual de Pesquisa Agropecuária, Eldorado do Sul, Eldorado do Sul, Rio Grande do Sul, Brazil.
Fabiana Q
FQ
Mayer
Departamento de Microbiologia Imunologia e Parasitologia - Laboratório de Virologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Paulo M
PM
Roehe
Laboratório de Virologia - Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Cláudio W
CW
Canal
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D021901:DNA, Intergenic / Q000235:genetics; D004848:Epithelium / Q000473:pathology / Q000821:virology; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D016013:Likelihood Functions; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D021901:DNA, Intergenic; D014764:Viral Proteins