Glycogen synthase kinase 3 (GSK3)-inhibitor SB216763 promotes the conversion of human umbilical cord mesenchymal stem cells into neural precursors in adherent culture.
The ability to generate neural progenitor cells from human umbilical cord mesenchymal stem cells (hUC-MSCs) has provided an option to treat neurodegenerative diseases. To establish a method for this purpose, we characterized the early neural markers of hUC-MSCs-derived cells under different conditions. We found that neither the elimination of signals for alternative fate nor N2 supplement was sufficient to differentiate hUC-MSCs into neural precursor cells, but the GSK3 inhibitor SB216763 could promote an efficient neural commitment of hUC-MSCs. The results indicated that Wnt/β-catenin might play an important role during the early neural differentiation of hUC-MSCs. Here, we report a method for hUC-MSCs to commit efficiently into a neural fate within a short period of time. This protocol provides an efficient method for hUC-MSCs-based neural regeneration.
Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China. pandarun@aliyun.com.
Liyang
L
Gao
Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Mingyan
M
Zhao
Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Peng
P
Li
Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Junchao
J
Kong
Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Zhijun
Z
Liu
Department of Pathology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Yonghua
Y
Chen
Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Rui
R
Huang
Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Jiaqi
J
Chu
Department of Gastroenterology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
Juanhua
J
Quan
Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China. 44147103@qq.com.
Rong
R
Zeng
D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics*; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors*; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D008301:Maleimides / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009416:Nerve Regeneration / Q000187:drug effects / Q000235:genetics; D009474:Neurons / Q000166:cytology*; D044968:Regenerative Medicine / Q000379:methods; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D013234:Stem Cells / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology*; D051153:Wnt Proteins / Q000502:physiology; D051176:beta Catenin / Q000502:physiology
D004791:Enzyme Inhibitors; D007211:Indoles; D008301:Maleimides; C417521:SB 216763; D051153:Wnt Proteins; D051176:beta Catenin; D038362:Glycogen Synthase Kinase 3
Default mechanism; GSK3 inhibitor; Human umbilical cord mesenchymal stem cells; N2 supplement; Neural differentiation; Neural precursor cells