The efficacy assessments of alkylating drugs induced by nano-Fe3O4/CA for curing breast and hepatic cancer.
A new method to evaluate the anticancer activity at the molecular level has been developed. In our assay, the interaction between alkylating anticancer drugs-Fe3O4/CA with DNA has been investigated for the Resonance Light Scattering (RLS) signal enhancement. Water-based nano-Fe3O4, as a probe, has the ability of good solubility, biodegradability and low bulk resistivity etc. The experimental results show that, the activity order of three kinds of drugs is Nimustine (ACNU)>Semustine (Me-CCNU)>Chlormethine (HN2), which is satisfied with the results of the cell apoptosis experiment and the IC50by MTT method. This assay is simple, sensitive and high efficient. And the theoretical basics for the development of new anticancer drugs as well as the assessments of their efficacy to cure breast and hepatic cancer have been provided.
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
Kui
K
He
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China; Avic Aviation Powerplant Research Institute, Zhuzhou, Hunan 412002, China.
Ying
Y
Ma
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
Bin
B
Yang
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
Caishuang
C
Liang
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
Xiaoming
X
Chen
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China. Electronic address: cai_mao3@hotmail.com.
Changqun
C
Cai
D018906:Antineoplastic Agents, Alkylating / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D019343:Citric Acid / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D015376:Nimustine / Q000494:pharmacology; D012673:Semustine / Q000494:pharmacology; D013055:Spectrophotometry, Infrared; D014783:Viscosity
D018906:Antineoplastic Agents, Alkylating; D004337:Drug Carriers; D005290:Ferric Compounds; D015376:Nimustine; D012673:Semustine; C000499:ferric oxide; D019343:Citric Acid; D004247:DNA
Anticancer drugs; Breast cancer; Cell apoptosis; Nano-Fe(3)O(4); Resonance light scattering (RLS)