Molecular Biological Methods in Environmental Engineering.
Bacteria, acting as catalysts, perform the function of degrading pollutants. Molecular biological techniques play an important role in research on the community analysis, the composition and the functions of complex microbial communities. The development of secondary high-throughput pyrosequencing techiniques enhances the understanding of the composition of the microbial community. The literatures of 2015 indicated that 16S rDNA gene as genetic tag is still the important method for bacteria identification and classification. 454 high throughput sequencing and Illumina MiSeq sequencing have been the primary and widely recognized methods to analyze the microbial. This review will provide environmental engineers and microbiologists an overview of important advancements in molecular techniques and highlight the application of these methods in diverse environments.
College of Forestry, Northeast Forestry University, Harbin, 150040, China.
Guocai
G
Zhang
Li
L
Wei
Chein-Chi
CC
Chang
Yuhua
Y
Zhang
Dong
D
Wei
D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D057005:Bioengineering / Q000379:methods*; D004785:Environmental Pollutants / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D012336:RNA, Ribosomal, 16S
D004785:Environmental Pollutants; D012336:RNA, Ribosomal, 16S