Process development for the production of bioethanol from waste algal biomass of Gracilaria verrucosa.
The algal biomass of different species of Gracilaria were collected from coasts of Orissa and Tamil Nadu, India and characterized biochemically. Among various species, G. verrucosa was found to be better in terms of total carbohydrate content (56.65%) and hence selected for further studies. The agar was extracted from algal biomass and the residual pulp was enzymatically hydrolyzed. The optimization of algal pulp hydrolysis for various parameters revealed a maximum sugar release of 75.8mg/ml with 63% saccharification yield. The fermentation of enzymatic hydrolysate of algal pulp was optimized and 8% (v/v) inoculum size, 12h inoculum age, pH 5.0 were found to be optimum parameters for maximum ethanol concentration (27.2g/L) after 12h. The process of enzymatic hydrolysis and fermentation were successfully scaled up to 2L bioreactor scale.
Central University of Haryana, Jant-Pali, Mahendergarh 123029, Haryana, India; Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India.
Rishikesh
R
Shukla
Marine Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi 110007, India.
Manoj
M
Kumar
Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India.
Subhojit
S
Chakraborty
Central University of Haryana, Jant-Pali, Mahendergarh 123029, Haryana, India; Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India.
Rishi
R
Gupta
Marine Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi 110007, India.
Savindra
S
Kumar
Marine Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi 110007, India.
Dinabandhu
D
Sahoo
Central University of Haryana, Jant-Pali, Mahendergarh 123029, Haryana, India; Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India. Electronic address: kuhad85@gmail.com.
Ramesh Chander
RC
Kuhad
D056804:Biofuels; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D044724:Gracilaria / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010316:Particle Size; D012624:Seaweed / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014866:Waste Products
D056804:Biofuels; D014866:Waste Products; D000431:Ethanol; D002480:Cellulase
Algal pulp; Bioethanol; Biorefinery; Fertilizer; Gracilaria verrucosa