Selection of specific inhibitor peptides in enzyme-linked immunosorbent assay (ELISA) of cardiac troponin I using immuno-dominant epitopes as competitor.
Human cardiac troponin I (cTni) is the gold marker for early diagnosis of myocardial infarction. In this regard, four immune-dominant epitopes of cTni were predicted and their 3D structures were determined. Thereafter, the competitive performance of the peptides was monitored with the developed polyclonal antibody-based indirect competitive ELISA; a half-maximal inhibitory concentration (IC50) of 0.49 (µg/mL) and detection limit of 0.037 (µg/mL) were achieved for recombinant cTni. The competitive ELISA determined sensitivity levels of 0.306, 0.141, 0.960, and 0.155 (µg/mL), respectively, for each peptide as competitor. We indicated that two of the selected epitopes have significant sensitivity scales and inhibition ability.
a Department of Clinical Biochemistry , Faculty of Medical Sciences, Tarbiat Modares University , Tehran , Iran.
Majid Asiabanha
MA
Rezaee
a Department of Clinical Biochemistry , Faculty of Medical Sciences, Tarbiat Modares University , Tehran , Iran.
Mohammad Javad
MJ
Rasaee
b Department of Life Science Engineering, Faculty of New Sciences and Technologies , University of Tehran , Tehran , Iran.
Javad
J
Mohammadnejad
D001667:Binding, Competitive / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D016056:Immunodominant Epitopes / Q000737:chemistry* / Q000276:immunology*; D007164:Immunosorbents / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D009203:Myocardial Infarction / Q000175:diagnosis / Q000276:immunology; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D019210:Troponin I / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*
D016056:Immunodominant Epitopes; D007164:Immunosorbents; D010455:Peptides; D019210:Troponin I
3D molecular structure; cardiac troponin I; enzyme-linked immunosorbent assay; immunodominant epitopes; molecular dynamics; polyclonal antibody