Photobiomodulation therapy on collagen type I and III, vascular endothelial growth factor, and metalloproteinase in experimentally induced tendinopathy in aged rats.
This study investigates the effect of photobiomodulation therapy (PBMT) on collagen type I and III, matrix metalloproteinase (MMP), and vascular endothelial growth factor (VEGF) in experimentally induced tendinopathy in female aged rats. Tendinopathy was induced by the Achilles tendoncollagenase peritendinous. Forty-two Wistar rats (Norvegicus albinus) were used; groups consisted of 36 aged animals (18 months old; mean body weight, 517.7 ± 27.54 g) and 6 adult animals (12 weeks old; mean body weight, 266± 19.30 g). The animals were divided into three groups: control, aged tendinopathy, and aged tendinopathy PBMT; the aged groups were subdivided based on time to euthanasia: 7, 14, and 21 days. PBMT involved a gallium-arsenide-aluminum laser (Theralaser, DMC®) with active medium operating at wavelength 830 ± 10 nm, 50 mW power, 0.028 cm2laser beam, 107 J/cm2energy density, 1.8 W/cm2power density, and an energy of 3 J per point. The laser was applied by direct contact with the left Achilles tendon during 60 s per point at a frequency of three times per week, until the euthanasia date (7, 14, and 21 days). VEGF, MMP-3, and MMP-9 were analyzed by immunohistochemistry, and collagen type I and III by Sirius red. PBMT increased the deposition of collagen type I and III in a gradual manner, with significant differences relative to the group aged tendonitis (p < 0.001), and in relation to VEGF (p < 0.001); decreased expression of MMP-3 and 9 were observed in group aged tendinopathy (p < 0.001). PBMT, therefore, increased the production of collagen type I and III, downregulated the expression of MMP-3 and MMP-9, and upregulated that of VEGF, with age and age-induced hormonal deficiency.
Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 235, São Paulo, SP, Brazil.
Anna Cristina de Farias
AC
Marques
Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 235, São Paulo, SP, Brazil.
Regiane
R
Albertini
Postgraduate Program in Biophotonics, Universidade Nove de Julho (UNINOVE), São Paulo, SP, Brazil.
Andrey Jorge
AJ
Serra
Postgraduate Program in Biophotonics, Universidade Nove de Julho (UNINOVE), São Paulo, SP, Brazil.
Evela Aparecida Pereira
EA
da Silva
Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 235, São Paulo, SP, Brazil.
Vanessa Lima Cavalcante
VL
de Oliveira
Postgraduate Program in Medicine, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
Luciana Miatto
LM
Silva
Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 235, São Paulo, SP, Brazil.|Postgraduate Program in Biophotonics, Universidade Nove de Julho (UNINOVE), São Paulo, SP, Brazil.
Ernesto Cesar Pinto
EC
Leal-Junior
Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 235, São Paulo, SP, Brazil. ptpaulo@terra.com.br.|Postgraduate Program in Biophotonics, Universidade Nove de Julho (UNINOVE), São Paulo, SP, Brazil. ptpaulo@terra.com.br.
Paulo de Tarso Camillo
PT
de Carvalho
D000125:Achilles Tendon; D000818:Animals; D024042:Collagen Type I / Q000528:radiation effects*; D024043:Collagen Type II / Q000528:radiation effects*; D004195:Disease Models, Animal; D005260:Female; D054023:Lasers, Semiconductor / Q000627:therapeutic use; D028022:Low-Level Light Therapy / Q000379:methods*; D019278:Matrix Metalloproteinase 3 / Q000528:radiation effects; D020780:Matrix Metalloproteinase 9 / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D052256:Tendinopathy / Q000532:radiotherapy*; D042461:Vascular Endothelial Growth Factor A / Q000528:radiation effects*
D024042:Collagen Type I; D024043:Collagen Type II; D042461:Vascular Endothelial Growth Factor A; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; D020780:Matrix Metalloproteinase 9
Aged; Calcaneal tendon; Collagenase; Photobiomodulation therapy; Tendinopathy