Microalgae pretreatment with liquid hot water to enhance enzymatic hydrolysis efficiency.
Nowadays, microalgae are being considered as promising raw material for bioethanol production. In this work, three process variables during liquid hot water (LHW) pretreatment prior to enzymatic hydrolysis by response surface methodology on Scenedesmus sp. WZKMT were investigated to enhance glucose recovery. Results indicated that the order of significance for three parameters was temperature>solid-to-liquid ratio>time. The optimal condition was 1:13 (w/v), 147°C and 40min. The concentration and recovery of glucose under this condition were 14.223g·L(-1) and 89.32%, respectively, which were up to 5-fold higher than the samples without LHW pretreatment. In addition, the surface morphologies of microalgae cells before and after LHW pretreatment were also verified using scanning electron microscopy (SEM). LHW pretreatment can greatly enhance the enzymatic efficiency, and can be regarded as an ideal pretreatment method for glucose recovery from microalgae.
Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Tao
T
Yuan
Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Xiekun
X
Li
Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Shiyuan
S
Xiao
Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China.
Ying
Y
Guo
Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China.
Weizheng
W
Zhou
Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address: xjl@ms.giec.ac.cn.
Jingliang
J
Xu
Guangzhou Institute of Energy Conversion, Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China.
Zhenhong
Z
Yuan
D002482:Cellulose / Q000378:metabolism; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D005947:Glucose; D006358:Hot Temperature; D006868:Hydrolysis; D058086:Microalgae / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D015203:Reproducibility of Results; D044444:Scenedesmus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors; D014867:Water / Q000494:pharmacology*
D014867:Water; D002482:Cellulose; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose
Bioethanol; Enzymatic hydrolysis; Glucose; Liquid hot water; Microalgae