Modulatory effects of interferon-γ and interleukin-4 on cellular immune responses against Hypoderma lineatum antigens.
This study investigates the in vitro modulatory effects of interferon-γ (IFN-γ) and interleukin-4 (IL-4) on both proliferative bovine T cell responses and IL-10 production induced by different antigens [crude larval extract and the purified fractions hypodermin A, B and C (HyA, HyB, HyC)] obtained from first instars of Hypoderma lineatum (Diptera: Oestridae), alone or in the presence of the mitogen concanavalin A. Incubation with the different parasitic antigens resulted in significant inhibition of T cell proliferation and IL-10 production, which, in general, did not revert after the addition of IFN-γ and IL-4. In the absence of antigens, IL-4 induced significant inhibition of mitogen-induced T cell responses. Exogenous IFN-γ exhibited an inhibitory effect on cell proliferation in the presence of the purified fractions HyB and HyC. These in vitro data suggest that far from neutralizing the effects of larval antigens, the addition of IFN-γ potentiates their anti-proliferative activity; by contrast, IL-4 had no consistent effects on proliferative responses to Hypoderma. IL-4 provoked an increment of IL-10 levels in supernatants of HyB-stimulated cells. In conclusion, exogenous IFN-γ and IL-4 were unable to counteract the suppressor effects of H. lineatum antigens.
Department of Animal Pathology, Sanidad Animal (INVESAGA Group), Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain.
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Cabanelas
Department of Animal Pathology, Sanidad Animal (INVESAGA Group), Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain.
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López
Department of Animal Pathology, Sanidad Animal (INVESAGA Group), Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain.
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Díaz
Department of Animal Pathology, Sanidad Animal (INVESAGA Group), Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain.
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Pérez-Creo
Department of Animal Pathology, Sanidad Animal (INVESAGA Group), Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain.
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Morrondo
Department of Animal Pathology, Sanidad Animal (INVESAGA Group), Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain.
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Díez-Baños
Department of Animal Pathology, Sanidad Animal (INVESAGA Group), Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain. rosario.panadero@usc.es.
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Panadero
D000818:Animals; D002417:Cattle / Q000276:immunology* / Q000469:parasitology; D004175:Diptera / Q000254:growth & development / Q000502:physiology*; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D007814:Larva / Q000254:growth & development / Q000502:physiology
D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma
Hypoderma; IFN-γ; IL-10; IL-4; cattle arthropods; cytokines; lymphoproliferation