Comparative Analysis of Carbon Monoxide Tolerance among Thermoanaerobacter Species.
An anaerobic thermophilic strain (strain PCO) was isolated from a syngas-converting enrichment culture. Syngas components cannot be used by strain PCO, but the new strain is very tolerant to carbon monoxide (pCO = 1.7 × 10(5) Pa, 100% CO). 16S rRNA gene analysis and DNA-DNA hybridization revealed that strain PCO is a strain of Thermoanaerobacter thermohydrosulfuricus. The physiology of strain PCO and other Thermoanaerobacter species was compared, focusing on their tolerance to carbon monoxide. T. thermohydrosulfuricus, T. brockii subsp. finnii, T. pseudethanolicus, and T. wiegelii were exposed to increased CO concentrations in the headspace, while growth, glucose consumption and product formation were monitored. Remarkably, glucose conversion rates by Thermoanaerobacter species were not affected by CO. All the tested strains fermented glucose to mainly lactate, ethanol, acetate, and hydrogen, but final product concentrations differed. In the presence of CO, ethanol production was generally less affected, but H2 production decreased with increasing CO partial pressure. This study highlights the CO resistance of Thermoanaerobacter species.
Centre of Biological Engineering, University of MinhoBraga, Portugal; Laboratory of Microbiology, Wageningen UniversityWageningen, Netherlands.
Joana I
JI
Alves
Centre of Biological Engineering, University of Minho Braga, Portugal.
M Madalena
MM
Alves
Laboratory of Microbiology, Wageningen University Wageningen, Netherlands.
Caroline M
CM
Plugge
Centre of Biological Engineering, University of MinhoBraga, Portugal; Laboratory of Microbiology, Wageningen UniversityWageningen, Netherlands.
Alfons J M
AJ
Stams
Centre of Biological Engineering, University of MinhoBraga, Portugal; Laboratory of Microbiology, Wageningen UniversityWageningen, Netherlands.
Diana Z
DZ
Sousa
Thermoanaerobacter; carbon monoxide; ethanol; hydrogen; sugar fermentation; syngas