Evaluation of in vitro urease and lipoxygenase inhibition activity of weight reducing tablets.
Enzyme inhibition is a significant part of research in pharmaceutical field in view of the fact that these studies have directed to the innovations of drugs having remarkable performance in diverse physiological conditions. The present study was aimed to assess urease and lipoxygenase inhibitory activity of weight reducing tablets. For evaluating the urease activity indophenol method was employed using Thiourea as the model urease inhibitor. The lipoxygenase inhibition was evaluated by measuring the hydroperoxides produced in lipoxygenation reaction using a purified lipoxygenase with lionoleic acid as substrate. When formulation of the weight reducing tablets was compared at various concentrations (50, 100 and 500µg/ml). The antiurease activity and lipoxygenase inhibition activity increased in a dose dependent manner. The formulations under test have an excellent antiurease and lipoxygenase inhibition potential and prospective to be used in the cure of a variety of complications associated with the production of urease and lipoxygenase enzymes.
Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan.
Syed Rashid Ali
SR
Jaffary
Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan.
Syed Waseemuddin
SW
Ahmed
Dow College of Pharmacy, Dow University of Health Sciences, Karachi, Pakistan.
Sadia
S
Shakeel
University College of Conventional Medicine, Faculty of Pharmacy Alternative Medicine, Islamia University, Bahawalpur, Pakistan.
Hafiz Muhammad
HM
Asif
Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan.
Khan
K
Usmanghani
D019440:Anti-Obesity Agents / Q000494:pharmacology*; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004339:Drug Compounding; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019787:Linoleic Acid / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D028321:Plant Preparations / Q000737:chemistry / Q000494:pharmacology; D011446:Prospective Studies; D013607:Tablets; D013890:Thiourea / Q000494:pharmacology; D014510:Urease / Q000037:antagonists & inhibitors*
D019440:Anti-Obesity Agents; D000897:Anti-Ulcer Agents; D004791:Enzyme Inhibitors; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D028321:Plant Preparations; D013607:Tablets; D019787:Linoleic Acid; D014510:Urease; D013890:Thiourea