Antioxidant activity of milk protein hydrolysate in alloxan-induced diabetic rats.
We investigated the effects of milk protein concentrate (MPC) and milk protein concentrate hydrolysate (MPCH) as antioxidant agents in rats. Six groups of healthy (non-diabetic) and type-II diabetic rats were used: (1) healthy rats (control), (2) alloxan-induced rats (diabetic control group), (3) healthy rats treated orally with MPC, (4) diabetic rats treated orally with MPC, (5) healthy rats treated orally with MPCH, and (6) diabetic rats treated orally with MPCH. We concluded that treatment with MPC or MPCH reduced the level of thiobarbituric acid reactive substances in healthy and diabetic rats. Treatment with MPC or MPCH improved activities of antioxidant enzymes (catalase, superoxide dismutase, reduced glutathione, glutathione-S-transferase, and glutathione peroxidase) in healthy and diabetic rats. From the present data, we concluded that both MPC and MPCH contain potent antioxidants and could improve the health of rats or other animals with diabetes mellitus.
Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Alexandria 21545, Egypt. Electronic address: sameh111eg@yahoo.com.
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Awad
Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Alexandria 21545, Egypt; Department of Dairy Technology Research, Food Technology Research Institute, ARC, Giza 12622, Egypt.
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El-Sayed
Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Alexandria 21545, Egypt.
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Wahba
Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Alexandria 21545, Egypt.
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El Attar
Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria 21526, Egypt.
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Yousef
Department of Dairy Technology Research, Food Technology Research Institute, ARC, Giza 12622, Egypt.
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Zedan
D000496:Alloxan; D000818:Animals; D000975:Antioxidants; D001786:Blood Glucose / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins; D011492:Protein Hydrolysates; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances
D000975:Antioxidants; D001786:Blood Glucose; D008894:Milk Proteins; D011492:Protein Hydrolysates; D017392:Thiobarbituric Acid Reactive Substances; D000496:Alloxan; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione
antioxidant enzyme; bioactive peptide; free radical; type-2 diabetes