Comparative characteristics of mesenchymal stem cells derived from reamer-irrigator-aspirator, iliac crest bone marrow, and adipose tissue.
Mesenchymal stem cells (MSCs) have been considered promising tools for new clinical concepts in supporting cellular therapy and regenerative medicine. More recently, Ream/Irrigator/Aspirator (RIA) was introduced as a source of MSCs. In this study we compared MSCs derived from three different sources (iliac crest bone marrow (ICBM), adipose tissue (AT), and (RIA)) regarding the morphology, the success rate of isolating MSCs, colony frequency, expansion potential, osteogenic and chondrogenic differentiation capacity. MSCs were isolated from three different sources and flow cytometric analyses were performed for cell characterization. Colony-forming unit-fibroblast (CFU-F) assay and population doubling time (PDT) were evaluated for MSCs derived from three different sources and differentiation potential of RIA, ICBM-, and AT-MSCs were determined by staining. Additionally, gene expression profiles for tissue specific markers corresponding to osteogenesis and chondrogenesis were analyzed using real time polymerase chain reaction (RT-PCR). Cultured with the appropriate condition, osteogenic and chondrogenic differentiation could be confirmed in all MSC preparations. Flow cytometry analysis indicated that RIA- and AT-derived MSCs have more homogenous populations than ICBM-MSCs. A comparison of the colonogenic ability in different tissues by CFU-F assay after 10 days showed that more colonies are formed from RIA-MSCs than from ICBM-MSCs, and AT-MSCs. AT-MSCs, were dispersed with no obvious colonies. The RIA-MSCs underwent osteogenesis and chondrogenesis at a faster rate than ICBM and AT-MSCs. Direct comparisons of RIA- to ICBM- and AT-MSCs have shown the RIA-MSCs have higher differentiation toward osteoblast and chondrocytes compared to other sources of MSCs. Hence, RIA-MSCs may be recommended as a more suitable source for treating orthopedic disorders.
Mashhad University of Medical Sciences Biotechnology Research Center, School of Pharmacy Mashhad Iran.
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S
Toosi
Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch Stem Cell and Regenerative Medicine Research Group Mashhad Iran.
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H
Naderi-Meshkin
Mashhad University of Medical Sciences Biotechnology Research Center, School of Pharmacy Mashhad Iran.
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F
Kalalinia
Mashhad University of Medical Sciences Department of Orthopedic Surgery, Orthopedic and Trauma Research Center Mashhad Iran.
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MT
Peivandi
National Yang Ming University School of Biomedical Engineering Taipei Taiwan.
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Hossein Khani
Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch Stem Cell and Regenerative Medicine Research Group Mashhad Iran.
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AR
Bahrami
Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch Stem Cell and Regenerative Medicine Research Group Mashhad Iran.
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A
Heirani-Tabasi
Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch Stem Cell and Regenerative Medicine Research Group Mashhad Iran.
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M
Mirahmadi
Mashhad University of Medical Sciences Biotechnology Research Center, School of Pharmacy Mashhad Iran Behravanj@mums.ac.ir.
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J
Behravan
D000273:Adipose Tissue / Q000166:cytology*; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D019070:Cell Lineage; D002462:Cell Membrane / Q000378:metabolism; D016922:Cellular Senescence; D019902:Chondrocytes / Q000166:cytology; D020219:Chondrogenesis; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D006801:Humans; D007085:Ilium / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D013396:Suction / Q000295:instrumentation*
D015415:Biomarkers