Thermoresponsive polyurethane/siloxane membrane for wound dressing and cell sheet transplantation: In-vitro and in-vivo studies.
Polyurethane/siloxane based wound dressing for transferring fibroblast cell sheet to wounded skin and ability to provide an optimum condition for cellular activity at damaged tissue was prepared in this research. The dressing was made thermoresponsive, via the introduction of a poly(N-isopropyl acrylamide) copolymer into the backbone of dressing. The ability of membrane for adhesion, growth, and proliferation of fibroblast cells was improved via surface modification with gelatin. The optimized dressing exhibited appropriate tensile strength (4.5MPa) and elongation at break (80%) to protect wound against physical forces. Due to controlled equilibrium water absorption of about 89% and water vapor transmission rate of 2040g/m(2)day, the dressing could maintain the favorable moist environment over moderate to high exuding wounds. The grown cell sheet on dressing membrane could easily roll up from the surface just with lowering the temperature. The in vivo study of the wound dressed with cell loaded membrane confirmed the accelerated healing and production of tissue with complete re-epithelization, enhanced vascularization, and increased collagen deposition on the damaged area.
Department of Tissue Engineering, School of Advanced Medical Technologies, Tehran University of Medical Sciences, 14177-55469 Tehran, Iran.
Alireza
A
Rezapour-Lactoee
Iran Polymer and Petrochemical Institute, P.O. Box: 14965/115, Tehran, Iran. Electronic address: h.yeganeh@ippi.ac.ir.
Hamid
H
Yeganeh
Department of Tissue Engineering, School of Advanced Medical Technologies, Tehran University of Medical Sciences, 14177-55469 Tehran, Iran; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, 16 Azar St, Enqelab Sq, Tehran 1417614411, Iran. Electronic address: ostadnas@sina.tums.ac.ir.
Seyed Nasser
SN
Ostad
Department of Tissue Engineering, School of Advanced Medical Technologies, Tehran University of Medical Sciences, 14177-55469 Tehran, Iran.
Reza
R
Gharibi
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Zohreh
Z
Mazaheri
Department of Tissue Engineering, School of Advanced Medical Technologies, Tehran University of Medical Sciences, 14177-55469 Tehran, Iran.
Jafar
J
Ai
D000818:Animals; D001458:Bandages; D002448:Cell Adhesion; D016923:Cell Death; D049109:Cell Proliferation; D017690:Cell Transplantation; D004548:Elasticity; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D008567:Membranes, Artificial; D011140:Polyurethanes / Q000737:chemistry*; D017208:Rats, Wistar; D012833:Siloxanes / Q000737:chemistry*; D013227:Steam; D013696:Temperature; D013718:Tensile Strength; D014783:Viscosity; D014945:Wound Healing
D008567:Membranes, Artificial; D011140:Polyurethanes; D012833:Siloxanes; D013227:Steam
Cell transplantation; Gelatin; Polyurethane/siloxane; Thermoresponsive; Wound healing