Induction of colitis in mice with food allergen-specific immune response.
The pathogenesis of intestinal chronic inflammation is unclear. Food allergy plays an important role in the induction of intestinal inflammation. This study aims to test a hypothesis that food allergy initiates colitis. In this study, BALB/c mice were sensitized to a common food allergen, ovalbumin (OVA) with cholera toxin (CT) as an adjuvant. The colon epithelial barrier function was assessed with Ussing chamber technique. Expression of T cell immunoglobulin mucin domain molecule-4 (TIM4) in dendritic cells was evaluated by flow cytometry, RT-PCR and Western blotting. The results showed that allergen-related colitis was induced in mice as shown by heavy infiltration of inflammatory cells in the colon mucosa, loss of body weight of mice, increases in myeloperoxidase, tumor necrosis factor-α, interleukin-4, OVA-specific IgE in the colon tissue. The colon epithelial barrier function was markedly compromised in colitis group mice, which was mimicked by exposure the colon mucosa to CT in Ussing chamber. High frequency of TIM4(+) dendritic cells was detected in the colon mucosa of colitis mice. Exposure of dendritic cells to CT markedly increased the expression of TIM4. We conclude that IBD-like inflammation can be induced in the mouse colon by the food allergen-related immune response.
The Department of Gastroenterology, the Second Hospital, Zhengzhou University, Zhengzhou, China.
Lin-Jing
LJ
Li
The Center of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.
Lu
L
Zeng
The Center of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.
Xiao-Xi
XX
Li
The Center of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Longgang ENT Hospital, Shenzhen ENT Institute, Shenzhen, China.
Li-Hua
LH
Mo
Longgang ENT Hospital, Shenzhen ENT Institute, Shenzhen, China.
Xiao-Rui
XR
Geng
The Department of Gastroenterology, the Fifth Hospital, Zhengzhou University, Zhengzhou, China.
Peng-Yuan
PY
Zheng
The Center of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.
Zhi-Gang
ZG
Liu
The Department of Gastroenterology, the Second Hospital, Zhengzhou University, Zhengzhou, China.
Bai-Sui
BS
Feng
The Center of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, China.
Ping-Chang
PC
Yang
D000818:Animals; D002772:Cholera Toxin / Q000276:immunology / Q000633:toxicity; D003092:Colitis / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D003106:Colon / Q000276:immunology / Q000473:pathology; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D005512:Food Hypersensitivity / Q000150:complications / Q000276:immunology*; D015212:Inflammatory Bowel Diseases / Q000209:etiology* / Q000276:immunology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008565:Membrane Proteins / Q000276:immunology; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000633:toxicity; D018418:Th2 Cells / Q000276:immunology / Q000473:pathology
D008565:Membrane Proteins; C500399:TIM-4 protein, mouse; D010047:Ovalbumin; D002772:Cholera Toxin