Elucidating the gut microbiome of ulcerative colitis: bifidobacteria as novel microbial biomarkers.
Ulcerative colitis (UC) is associated with a substantial alteration of specific gut commensals, some of which may be involved in microbiota-mediated protection. In this study, microbiota cataloging of UC patients by 16S rRNA microbial profiling revealed a marked reduction of bifidobacteria, in particular the Bifidobacterium bifidum species, thus suggesting that this taxon plays a biological role in the aetiology of UC. We investigated this further through an in vivo trial by testing the effects of oral treatment with B. bifidum PRL2010 in a wild-type murine colitis model. TNBS-treated mice receiving 10(9) cells of B. bifidum PRL2010 showed a marked reduction of all colitis-associated histological indices as well as maintenance of mucosal integrity as it was shown by the increase in the expression of many tight junction-encoding genes. The protective role of B. bifidum PRL2010, as well as its sortase-dependent pili, appears to be established through the induction of an innate immune response of the host. These results highlight the importance of B. bifidum as a microbial biomarker for UC, revealing its role in protection against experimentally induced colitis.
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parco Area delle Scienze 11A, 43124 Parma, Italy.
Sabrina
S
Duranti
Gastroenterology Unit, University Hospital of Parma, Via Gramsci 14, 43126 Parma, Italy.
Federica
F
Gaiani
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parco Area delle Scienze 11A, 43124 Parma, Italy.
Leonardo
L
Mancabelli
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parco Area delle Scienze 11A, 43124 Parma, Italy.
Christian
C
Milani
Department of Pharmacy, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy.
Andrea
A
Grandi
Laboratory of Molecular Biology, Department of Life Sciences, University of Parma, Parco Area delle Scienze 11/A, 43124 Parma, Italy.
Angelo
A
Bolchi
Laboratory of Molecular Biology, Department of Life Sciences, University of Parma, Parco Area delle Scienze 11/A, 43124 Parma, Italy.
Andrea
A
Santoni
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parco Area delle Scienze 11A, 43124 Parma, Italy.
Gabriele Andrea
GA
Lugli
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parco Area delle Scienze 11A, 43124 Parma, Italy.
Chiara
C
Ferrario
GenProbio srl, Parco Area delle Scienze 11A, 43124 Parma, Italy.
Marta
M
Mangifesta
GenProbio srl, Parco Area delle Scienze 11A, 43124 Parma, Italy.
Alice
A
Viappiani
Department of Pharmacy, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy.
Simona
S
Bertoni
Department of Pharmacy, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy.
Valentina
V
Vivo
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parco Area delle Scienze 11A, 43124 Parma, Italy.
Fausta
F
Serafini
Department of Medical and Surgical Sciences and Center for Applied Biomedical Research (CRBA), University of Bologna, Via Massarenti 9, Bologna 40138, Italy.
Maria Raffaella
MR
Barbaro
Gastroenterology Unit, University Hospital of Parma, Via Gramsci 14, 43126 Parma, Italy.
Alessandro
A
Fugazza
Department of Medical and Surgical Sciences and Center for Applied Biomedical Research (CRBA), University of Bologna, Via Massarenti 9, Bologna 40138, Italy.
Giovanni
G
Barbara
Department of Neurosciences, University of Parma, 43124 Parma, Italy.
Laura
L
Gioiosa
Department of Neurosciences, University of Parma, 43124 Parma, Italy.
Paola
P
Palanza
Department of Veterinary Science, University of Parma, Via del Taglio 10, 43126 Parma, Italy.
Anna Maria
AM
Cantoni
Gastroenterology Unit, University Hospital of Parma, Via Gramsci 14, 43126 Parma, Italy.
Gian Luigi
GL
de'Angelis
Department of Pharmacy, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy.
Elisabetta
E
Barocelli
Unit of Digestive, Hepato-Pancreato-Biliary Surgery and Liver Transplantation, Henri Mondor Hospital, 51 Avenue du Maréchal de Lattre de Tassigny, Créteil 94010, France.
Nicola
N
de'Angelis
APC Microbiome Institute and School of Microbiology, National University of Ireland, Cork, Ireland.
Douwe
D
van Sinderen
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parco Area delle Scienze 11A, 43124 Parma, Italy.
Marco
M
Ventura
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parco Area delle Scienze 11A, 43124 Parma, Italy francesca.turroni@gmail.com.
Francesca
F
Turroni
D000818:Animals; D001644:Bifidobacterium / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D015415:Biomarkers; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000382:microbiology*; D064806:Dysbiosis / Q000382:microbiology*; D005260:Female; D010861:Fimbriae, Bacterial / Q000276:immunology*; D000069196:Gastrointestinal Microbiome / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology
D015415:Biomarkers; D012336:RNA, Ribosomal, 16S
dysbiosis; gut microbiota; intestinal inflammation; metagenomics; probiotic bacteria; ulcerative colitis