In vitro study of antioxidant activity and phenolic content of Chrysanthemum balsamita varieties.
The purpose of our study was to identify the phenolic substances of two varieties of Chrysanthemum balsamita (balsamita and tanacetoides) and to measure the overall antioxidant activity. The phenolic compounds were determined by HPLC. The evaluation of the polyphenolic content was performed by colorimetric analysis. The antioxidant activity was measured by three in vitro assay models: the DPPH, the silver nanoparticles antioxidant capacity (SNPAC) and EPR radical detection. Using HPLC-MS analysis, phenolic acids, flavonoids and flavonoid aglycone were detected. The highest antioxidant activity was showed by Chrysanthemum balsamita var. balsamita, while the lowest for the Chrysanthemum balsamita var. tanacetoides extract, in accord with the polyphenolic content. The results show that Chrysanthemum balsamita var. balsamita might be a source of antioxidant flavonoids, especially rutin and isoquercitrin.
Iuliu Hatieganu University of Medicine and Pharmacy, 8, V. Babes Street, Cluj-Napoca, Romania.
Daniela
D
Benedec
Iuliu Hatieganu University of Medicine and Pharmacy, 8, V. Babes Street, Cluj-Napoca, Romania.
Lorena
L
Filip
Iuliu Hatieganu University of Medicine and Pharmacy, 8, V. Babes Street, Cluj-Napoca, Romania.
Laurian
L
Vlase
University of Agricultural Sciences and Veterinary Medicine, 3-5, Mănăştur Street, Cluj-Napoca, Romania.
Constantin
C
Bele
University of Agricultural Sciences and Veterinary Medicine, 3-5, Mănăştur Street, Cluj-Napoca, Romania.
Bogdan
B
Sevastre
National Institute for Research and Development of Isotopic and Molecular Technologies, Donath Street, Cluj-Napoca.
Oana
O
Raita
Institute of Life Sciences, Western University, Vasile Goldiş, Rebreanu Street, Arad.
Neli-Kinga
NK
Olah
Iuliu Hatieganu University of Medicine and Pharmacy, 8, V. Babes Street, Cluj-Napoca, Romania.
Daniela
D
Hanganu
D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D031202:Chrysanthemum / Q000737:chemistry*; D003124:Colorimetry; D053758:Nanoparticles; D010636:Phenols / Q000032:analysis*; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012834:Silver; D013045:Species Specificity
D000975:Antioxidants; D001713:Biphenyl Compounds; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D012834:Silver; C004931:1,1-diphenyl-2-picrylhydrazyl