The effect of glucose-coated gold nanoparticles on radiation bystander effect induced in MCF-7 and QUDB cell lines.
Due to biocompatibility and relative non-toxic nature, gold nanoparticles (GNPs) have been studied widely to be employed in radiotherapy as radio-sensitizer. On the other hand, they may enhance radiation-induced bystander effect (RIBE), which causes radiation adverse effects in non-irradiated normal cells. The present study was planned to investigate the possibility of augmenting the RIBE consequence of applying glucose-coated gold nanoparticles (Glu-GNPs) to target cells. Glu-GNPs were synthesized and utilized to treat MCF7 and QUDB cells. The treated cells were irradiated with 100 kVp X-rays, and their culture media were transferred to non-irradiated bystander cells. Performing MTT cellular proliferation test and colony formation assay, percentage cell viability and survival fraction of bystander cells were determined, respectively, and were compared to control bystander cells which received culture medium from irradiated cells without Glu-GNPs. Glu-GNPs decreased the cell viability and survival fraction of QUDB bystander cells by as much as 13.2 and 11.5 %, respectively (P < 0.02). However, the same end points were not changed by Glu-GNPs in MCF-7 bystander cells. Different RIBE responses were observed in QUDB and MCF7 loaded with Glu-GNPs. Glu-GNPs increased the RIBE in QUDB cells, while they had no effects on RIBE in MCF7 cells. As opposed to QUDB cells, the RIBE in MCF7 cells did not change in the dose range of 0.5-10 Gy. Therefore, it might be a constant effect and the reason of not being increased by Glu-GNPs.
Department of Medical Physic, Faculty of Medicine, Mashhad University of Medical Sciences, Pardis Daneshgah, Mashhad, 91779-48564, Iran.
Atefeh
A
Rostami
Department of Medical Physic, Faculty of Medicine, Mashhad University of Medical Sciences, Pardis Daneshgah, Mashhad, 91779-48564, Iran.|Medical Physics Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Mohammad Thaghi Bahreyni
MT
Toossi
Department of Medical Physic, Faculty of Medicine, Mashhad University of Medical Sciences, Pardis Daneshgah, Mashhad, 91779-48564, Iran.|Medical Physics Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Ameneh
A
Sazgarnia
Department of Medical Physic, Faculty of Medicine, Mashhad University of Medical Sciences, Pardis Daneshgah, Mashhad, 91779-48564, Iran. soleymanifardsh@mums.ac.ir.|Medical Physics Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. soleymanifardsh@mums.ac.ir.|Medical Physics Department, Omid hospital, Mashhad, Iran. soleymanifardsh@mums.ac.ir.
Shokouhozaman
S
Soleymanifard
D001692:Biological Transport / Q000187:drug effects / Q000528:radiation effects; D024201:Bystander Effect / Q000187:drug effects* / Q000528:radiation effects*; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*
D006046:Gold; D005947:Glucose
Gold nanoparticles; MCF-7 cell line; QU-DB cell line; Radiation-induced bystander effect; Radiotherapy