Cultures and co-cultures of human blood mononuclear cells and endothelial cells for the biocompatibility assessment of surface modified AISI 316L austenitic stainless steel.
Samples of AISI 316L austenitic stainless steel were subjected either to grinding and polishing procedure, or to grinding and then low temperature glow-discharge nitriding treatment, or to grinding, nitriding and subsequently coating with collagen-I. Nitrided samples, even if only ground, show a higher corrosion resistance in PBS solution, in comparison with ground and polished AISI 316L. Biocompatibility was evaluated in vitro by incubating the samples with either peripheral blood mononuclear cells (PBMC) or human umbilical vein endothelial cells (HUVEC), tested separately or in co-culture. HUVEC-PBMC co-culture and co-incubation of HUVEC with PBMC culture medium, after the previous incubation of PBMC with metallic samples, allowed to determine whether the incubation of PBMC with the different samples might affect HUVEC behaviour. Many biological parameters were considered: cell proliferation, release of cytokines, matrix metalloproteinases (MMPs) and sICAM-1, gelatinolytic activity of MMPs, and ICAM-1 protein expression. Nitriding treatment, with or without collagen coating of the samples, is able to ameliorate some of the biological parameters taken into account. The obtained results point out that biocompatibility may be successfully tested in vitro, using cultures of normal human cells, as blood and endothelial cells, but more than one cell line should be used, separately or in co-culture, and different parameters should be determined, in particular those correlated with inflammatory phenomena.
Dipartimento di Scienze Biomediche, Sperimentali e Cliniche 'Mario Serio', Sezione di Scienze Biochimiche, Università di Firenze, viale Morgagni 50, 50134 Firenze, Italy.
Maria
M
Stio
Dipartimento di Scienze Biomediche, Sperimentali e Cliniche 'Mario Serio', Sezione di Scienze Biochimiche, Università di Firenze, viale Morgagni 50, 50134 Firenze, Italy.
Maria
M
Martinesi
Dipartimento di Scienze Biomediche, Sperimentali e Cliniche 'Mario Serio', Sezione di Scienze Biochimiche, Università di Firenze, viale Morgagni 50, 50134 Firenze, Italy.
Cristina
C
Treves
Dipartimento di Ingegneria Industriale (DIEF), Università di Firenze, via S. Marta 3, 50139 Firenze, Italy. Electronic address: francesca.borgioli@unifi.it.
Francesca
F
Borgioli
D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D024042:Collagen Type I / Q000378:metabolism; D003343:Corrosion; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016207:Cytokines / Q000032:analysis; D058266:Dielectric Spectroscopy; D004797:Enzyme-Linked Immunosorbent Assay; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism; D009584:Nitrogen / Q000737:chemistry; D013193:Stainless Steel / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D024042:Collagen Type I; D017077:Culture Media, Conditioned; D016207:Cytokines; C426023:austenitic steel; D013193:Stainless Steel; D018799:Intercellular Adhesion Molecule-1; D020782:Matrix Metalloproteinases; D009584:Nitrogen
Austenitic stainless steel; Biocompatibility; Human cell cultures; Inflammation; Surface modification