A novel high-throughput multi-parameter flow cytometry based method for monitoring and rapid characterization of microbiome dynamics in anaerobic systems.
A novel multidimensional flow cytometry based method has been demonstrated to monitor and rapidly characterize the dynamics of the complex anaerobic microbiome associated with perturbations in external environmental factors. While community fingerprinting provides an estimate of the meta genomic structure, flow cytometry provides a fingerprint of the community morphology including its autofluorescence spectrum in a high-throughput manner. Using anaerobic microbial consortia perturbed with the controlled addition of various carbon sources, it is possible to quantitatively discriminate between divergent microbiome analogous to community fingerprinting techniques using automated ribosomal intergenic spacer analysis (ARISA). The utility of flow cytometry based method has also been demonstrated in a fully functional industry scale anaerobic digester to distinguish between microbiome composition caused by varying hydraulic retention time (HRT). This approach exploits the rich multidimensional information from flow cytometry for rapid characterization of the dynamics of microbial communities.
Department of Agricultural and Biological Engineering, University of Illinois, Urbana-Champaign, 1304 W Pennsylvania Ave, Urbana, IL 61801, USA. Electronic address: dhoble2@illinois.edu.
Abhishek S
AS
Dhoble
Department of Agricultural and Biological Engineering, University of Illinois, Urbana-Champaign, 1304 W Pennsylvania Ave, Urbana, IL 61801, USA. Electronic address: sbekal8@gmail.com.
Sadia
S
Bekal
Tempus Health, Inc., 600 W Chicago St, Floor 7 Light Bank Office, Chicago, IL 60654, USA. Electronic address: will@tempus.com.
William
W
Dolatowski
SunPower Corporation, 1504 Eureka Rd Suite 130, Roseville, CA 95661, USA. Electronic address: connor.yanz@gmail.com.
Connor
C
Yanz
Department of Crop Sciences, University of Illinois, Urbana-Champaign, N-423 Turner Hall 1102 S. Goodwin, Urbana 61801, USA. Electronic address: knlamber@illinois.edu.
Kris N
KN
Lambert
Department of Agricultural and Biological Engineering, University of Illinois, Urbana-Champaign, 1304 W Pennsylvania Ave, Urbana, IL 61801, USA. Electronic address: bhalerao@illinois.edu.
Kaustubh D
KD
Bhalerao
D000693:Anaerobiosis; D001331:Automation; D019149:Bioreactors / Q000382:microbiology; D016000:Cluster Analysis; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D019992:Databases as Topic; D005434:Flow Cytometry / Q000379:methods*; D059013:Microbial Consortia / Q000235:genetics; D064307:Microbiota / Q000235:genetics; D013997:Time Factors
D021903:DNA, Ribosomal Spacer
Anaerobic digestion; Autofluorescence; Flow cytometry; Labe free monitoring; Microbial community; Microbial dynamics; Microbiome characterization