Furfuryl methacrylate plasma polymers for biomedical applications.
Furfuryl methacrylate (FMA) is a promising precursor for producing polymers for biomedical and cell therapy applications. Herein, FMA plasma polymer coatings were prepared with different powers, deposition times, and flow rates. The plasma polymer coatings were characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS), and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The results from AFM and SEM show the early growth of the coatings and the existence of particle aggregates on the surfaces. XPS results indicated no measureable chemical differences between the deposited films produced under different power and flow rate conditions. ToF-SIMS analysis demonstrated differing amounts of C5H5O (81 m/z) and C10H9O2 (161 m/z) species in the coatings which are related to the furan ring structure. Through judicious choice of plasma polymerization parameters, the quantity of the particle aggregates was reduced, and the fabricated plasma polymer coatings were chemically uniform and smooth. Primary human fibroblasts were cultured on FMA plasma polymer surfaces to determine the effect of surface chemical composition and the presence of particle aggregates on cell culture. Particle aggregates were shown to inhibit fibroblast attachment and proliferation.
Future Industries Institute, University of South Australia, Mawson Lakes, Adelaide, South Australia 5095, Australia and Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM), Mawson Lakes, South Australia 5095, Australia.
Hanieh Safizadeh
HS
Shirazi
Future Industries Institute, University of South Australia, Mawson Lakes, Adelaide, South Australia 5095, Australia and Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM), Mawson Lakes, South Australia 5095, Australia.
Nicholas
N
Rogers
Future Industries Institute, University of South Australia, Mawson Lakes, Adelaide, South Australia 5095, Australia; Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM), Mawson Lakes, South Australia 5095, Australia; and School of Engineering, University of South Australia, Mawson Lakes, Adelaide, South Australia 5095, Australia.
Andrew
A
Michelmore
Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM), Mawson Lakes, South Australia 5095, Australia and School of Engineering, University of South Australia, Mawson Lakes, Adelaide, South Australia 5095, Australia.
Jason D
JD
Whittle
D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D005347:Fibroblasts / Q000502:physiology; D005663:Furans / Q000378:metabolism*; D006801:Humans; D008689:Methacrylates / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000378:metabolism*; D018629:Spectrometry, Mass, Secondary Ion; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D005663:Furans; D008689:Methacrylates; D011108:Polymers; C085853:furfuryl methacrylate