Curcumin-loaded embryonic stem cell exosomes restored neurovascular unit following ischemia-reperfusion injury.
We tested whether the combined nano-formulation, prepared with curcumin (anti-inflammatory and neuroprotective molecule) and embryonic stem cell exosomes (MESC-exocur), restored neurovascular loss following an ischemia reperfusion (IR) injury in mice. IR-injury was created in 8-10 weeks old mice and divided into two groups. Out of two IR-injured groups, one group received intranasal administration of MESC-exocurfor 7days. Similarly, two sham groups were made and one group received MESC-exocurtreatment. The study determined that MESC-exocurtreatment reduced neurological score, infarct volume and edema following IR-injury. As compared to untreated IR group, MESC-exocurtreated-IR group showed reduced inflammation and N-methyl-d-aspartate receptor expression. Treatment of MESC-exocuralso reduced astrocytic GFAP expression and alleviated the expression of NeuN positive neurons in IR-injured mice. In addition, MESC-exocurtreatment restored vascular endothelial tight (claudin-5 and occludin) and adherent (VE-cadherin) junction proteins in IR-injured mice as compared to untreated IR-injured mice. These results suggest that combining the potentials of embryonic stem cell exosomes and curcumin can help neurovascular restoration following ischemia-reperfusion injury in mice.
Department of Physiology, School of Medicine, University of Louisville, KY, USA. Electronic address: a0kala02@louisville.edu.
Anuradha
A
Kalani
Department of Physiology, School of Medicine, University of Louisville, KY, USA.
Pankaj
P
Chaturvedi
Department of Physiology, School of Medicine, University of Louisville, KY, USA.
Pradip K
PK
Kamat
Department of Physiology, School of Medicine, University of Louisville, KY, USA; Department of Surgery, University of Louisville, KY, USA.
Claudio
C
Maldonado
Department of Surgery, University of Louisville, KY, USA.
Philip
P
Bauer
Department of Physiology, School of Medicine, University of Louisville, KY, USA.
Irving G
IG
Joshua
Department of Physiology, School of Medicine, University of Louisville, KY, USA.
Suresh C
SC
Tyagi
Department of Physiology, School of Medicine, University of Louisville, KY, USA.
Neetu
N
Tyagi
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D004339:Drug Compounding; D053595:Embryonic Stem Cells / Q000166:cytology*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D055354:Exosomes / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D015427:Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology* / Q000628:therapy*; D062725:Tight Junction Proteins / Q000378:metabolism
D015815:Cell Adhesion Molecules; D018696:Neuroprotective Agents; D062725:Tight Junction Proteins; D003474:Curcumin
Curcumin; Exosomes; Neurons; Stroke