Isolation and characterisation of lactic acid bacteria from donkey milk.
During the last years the interest in donkey milk has increased significantly mainly because of its compelling functional elements. Even if the composition and nutritional properties of donkey milk are known, its microbiota is less studied. This Research Communication aimed to provide a comprehensive characterisation of the lactic acid bacteria in raw donkey milk. RAPD-PCR assay combined with 16S rDNA sequencing analysis were used to describe the microbial diversity of several donkey farms in the North West part of Italy. The more frequently detected species were: Lactobacillus paracasei, Lactococcus lactis and Carnobacterium maltaromaticum. Less abundant genera were Leuconostoc, Enterococcus and Streptococcus. The yeast Kluyveromyces marxianus was also isolated. The bacterial and biotype distribution notably diverged among the farms. Several of the found species, not previously detected in donkey milk, could have an important probiotic activity and biotechnological potential. This study represents an important insight to the ample diversity of the microorganisms present in the highly selective ecosystem of raw donkey milk.
Dipartimento di Scienze Veterinarie,Università di Torino,Largo Braccini 2,10095 Grugliasco (TO),Italy.
Maria de Los Dolores
Mde L
Soto Del Rio
Veneto Agricoltura,Istituto per la Qualità e le Tecnologie Agroalimentari,Via San Gaetano 74,36016 Thiene (VI),Italy.
Christian
C
Andrighetto
Dipartimento di Scienze Veterinarie,Università di Torino,Largo Braccini 2,10095 Grugliasco (TO),Italy.
Alessandra
A
Dalmasso
Veneto Agricoltura,Istituto per la Qualità e le Tecnologie Agroalimentari,Via San Gaetano 74,36016 Thiene (VI),Italy.
Angiolella
A
Lombardi
Dipartimento di Scienze Veterinarie,Università di Torino,Largo Braccini 2,10095 Grugliasco (TO),Italy.
Tiziana
T
Civera
Dipartimento di Scienze Veterinarie,Università di Torino,Largo Braccini 2,10095 Grugliasco (TO),Italy.
Maria Teresa
MT
Bottero
D000818:Animals; D044822:Biodiversity; D056573:Carnobacterium / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D017753:Ecosystem; D018397:Equidae / Q000382:microbiology*; D007558:Italy; D007716:Kluyveromyces / Q000302:isolation & purification; D007777:Lactobacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification; D013294:Lactococcus lactis / Q000235:genetics / Q000302:isolation & purification; D008892:Milk / Q000382:microbiology*; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
Donkey milk; RAPD-PCR characterisation; lactic acid bacteria