Effect of Korean Red Ginseng extraction conditions on antioxidant activity, extraction yield, and ginsenoside Rg1 and phenolic content: optimization using response surface methodology.
BACKGROUND
Extraction conditions greatly affect composition, as well as biological activity. Therefore, optimization is essential for maximum efficacy.


METHODS
Korean Red Ginseng (KRG) was extracted under different conditions and antioxidant activity, extraction yield, and ginsenoside Rg1 and phenolic content evaluated. Optimized extraction conditions were suggested using response surface methodology for maximum antioxidant activity and extraction yield.


RESULTS
Analysis of KRG extraction conditions using response surface methodology showed a good fit of experimental data as demonstrated by regression analysis. Among extraction factors, such as extraction solvent and extraction time and temperature, ethanol concentration greatly affected antioxidant activity, extraction yield, and ginsenoside Rg1 and phenolic content. The optimal conditions for maximum antioxidant activity and extraction yield were an ethanol concentration of 48.8%, an extraction time 73.3 min, and an extraction temperature of 90°C. The antioxidant activity and extraction yield under optimal conditions were 43.7% and 23.2% of dried KRG, respectively.


CONCLUSIONS
Ethanol concentration is an important extraction factor for KRG antioxidant activity and extraction yield. Optimized extraction conditions provide useful economic advantages in KRG development for functional products.
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Jin Woo
JW
Lee
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Eun Jin
EJ
Mo
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Ji Eun
JE
Choi
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Yang Hee
YH
Jo
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Hari
H
Jang
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Ji Yeon
JY
Jeong
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Qinghao
Q
Jin
Dasom Co., Ltd, Cheongju, Korea.
Hee Nam
HN
Chung
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Bang Yeon
BY
Hwang
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Mi Kyeong
MK
Lee
Panax ginseng (Korean Red Ginseng); antioxidant activity; extraction conditions; extraction yield; optimization