Hybrid Cross-Linked Lipase Aggregates with Magnetic Nanoparticles: A Robust and Recyclable Biocatalysis for the Epoxidation of Oleic Acid.
Highly stable and easily recyclable hybrid magnetic cross-linked lipase aggregates (HM-CSL-CLEAs) were prepared by coaggregation of lipase aggregates with nonfunctionalized magnetic nanoparticles and subsequent chemical cross-linking with glutaraldehyde. Analysis by SEM and CLSM indicated that the CLEAs were embedded in nanoparticle aggregates instead of covalently immobilized. The resulting HM-CSL-CLEAs exhibited higher thermostability, storage stability, and reusability than standard CLEAs. For example, HM-CSL-CLEAs maintained >60% of their initial activity after 40 min of incubation at 60 °C, whereas standard CLEAs lost most of their activities. The HM-CSL-CLEAs can be easily recovered from the reaction mixture by an external magnetic field. Moreover, the H2O2 tolerance of the lipase in HM-CSL-CLEAs was also enhanced, which could relieve the inhibitory effect on lipase activity. A high conversion yield (55%) for the epoxidation of oleic acid using H2O2 as oxidizing agent was achieved by HM-CSL-CLEAs.
Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology , Shijiazhang, People's Republic of China.|Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology , No. 29, 13th, Avenue, Tianjin Economic and Technological Development Area (TEDA), Tianjin 300457, People's Republic of China.
Jiandong
J
Cui
Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology , Shijiazhang, People's Republic of China.
Lili
L
Cui
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology , No. 29, 13th, Avenue, Tianjin Economic and Technological Development Area (TEDA), Tianjin 300457, People's Republic of China.
Shiru
S
Jia
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences , Beijing, People's Republic of China.
Zhiguo
Z
Su
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences , Beijing, People's Republic of China.
Songping
S
Zhang
D055162:Biocatalysis; D003432:Cross-Linking Reagents / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D019301:Oleic Acid / Q000737:chemistry*; D013696:Temperature
D003432:Cross-Linking Reagents; D058185:Magnetite Nanoparticles; D019301:Oleic Acid; D006861:Hydrogen Peroxide; D008049:Lipase; D005976:Glutaral
cross-linked enzyme aggregates; enzyme immobilization; epoxidation; lipase; magnetic nanoparticles