Wolbachia Has Two Different Localization Patterns in Whitefly Bemisia tabaci AsiaII7 Species.
The whitefly Bemisia tabaci is a cosmopolitan insect species complex that harbors the obligate primary symbiont Portiera aleyrodidarum and several facultative secondary symbionts including Wolbachia, which have diverse influences on the host biology. Here, for the first time, we revealed two different localization patterns of Wolbachia present in the immature and adult stages of B. tabaci AsiaII7 cryptic species. In the confined pattern, Wolbachia was restricted to the bacteriocytes, while in the scattered pattern Wolbachia localized in the bacteriocytes, haemolymph and other organs simultaneously. Our results further indicated that, the proportion of B. tabaci AsiaII7 individuals with scattered Wolbachia were significantly lower than that of confined Wolbachia, and the distribution patterns of Wolbachia were not associated with the developmental stage or sex of whitefly host. This study will provide a new insight into the various transmission routes of Wolbachia in different whitefly species.
Department of Entomology, South China Agricultural University, Guangzhou, China.|Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, Guangzhou, China.
Peiqiong
P
Shi
Department of Entomology, South China Agricultural University, Guangzhou, China.|Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, Guangzhou, China.
Zhan
Z
He
Department of Entomology, South China Agricultural University, Guangzhou, China.|Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, Guangzhou, China.
Shaojian
S
Li
Department of Entomology, South China Agricultural University, Guangzhou, China.|Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, Guangzhou, China.
Xuan
X
An
Department of Entomology, South China Agricultural University, Guangzhou, China.|Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, Guangzhou, China.
Ning
N
Lv
Department of Entomology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
Murad
M
Ghanim
Fera, Sand Hutton, York, United Kingdom.
Andrew G S
AG
Cuthbertson
Department of Entomology, South China Agricultural University, Guangzhou, China.|Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, Guangzhou, China.
Shun-Xiang
SX
Ren
Department of Entomology, South China Agricultural University, Guangzhou, China.|Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, Guangzhou, China.
Bao-Li
http://orcid.org/0000-0002-0735-013X
BL
Qiu
D000818:Animals; D004531:Eggs / Q000382:microbiology; D005260:Female; D006430:Hemiptera / Q000382:microbiology*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D020577:Wolbachia / Q000502:physiology*