Dataset of differential lipid raft and global proteomes of SILAC-labeled cystic fibrosis cells upon TNF -α stimulation.
Cystic fibrosis (CF) is a genetic disease due to mutations in the cystic fibrosis transmembrane regulator (CFTR), F508del-CFTR being the most frequent. Lipid raft-like microdomains (LRM) are regions of the plasma membrane that present a high cholesterol content and are insoluble to non-ionic detergents. LRM are essential functional and structural platforms that play an important role in the inflammatory response. CFTR is a known modulator of inflammation in LRM. Here we provide mass spectrometry data on the global impact of CFTR mutation and TNF-a stimulation on the LRM proteome. We used the Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) approach to quantify and identify 332 proteins in LRM upon TNF-a stimulation in CF cells and 1381 for the global proteome. We report two detailed tables containing lists of proteins obtained by mass spectrometry and the immunofluorescence validation results for one of these proteins, the G-protein coupled receptor 5A. These results are associated with the article "Changes in lipid raft proteome upon TNF-α stimulation of cystic fibrosis cells" (Chhuon et al., in press [1]).
Proteomic Platform Necker, PPN-3P5, Structure Fédérative de Recherche SFR Necker US24, 75015 Paris, France.
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Chhuon
Institut Necker Enfants Malades, INSERM, U1151, Paris, France.
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Pranke
Institut Necker Enfants Malades, INSERM, U1151, Paris, France.
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Borot
Institut Necker Enfants Malades, INSERM, U1151, Paris, France.
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Tondelier
The CPN Proteomics Facility - 3P5, Center of Psychiatry and Neuroscience, UMR INSERM 894, 75014 Paris, France.
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Lipecka
Institut Necker Enfants Malades, INSERM, U1151, Paris, France.
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Fritsch
Geneva University Hospitals and University of Geneva, 1211 Geneva, Switzerland.
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Chanson
Institut Necker Enfants Malades, INSERM, U1151, Paris, France.
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Edelman
Institut Mondor de Recherche Biomédicale, INSERM, U955, and Université Paris Est Créteil, 94010 Créteil, France.
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Ollero
Proteomic Platform Necker, PPN-3P5, Structure Fédérative de Recherche SFR Necker US24, 75015 Paris, France.
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Guerrera