Investigation into Iron Chelating and Antioxidant Potential of Melilotus officinalis in Iron Dextran Induced Iron Overloaded Sprague Dawley Rat Model.
Excess of iron leads to generates free radicals, causes organ damage.Melilotus officinalis(Fabaceae) reported to have various pharmacological activities. It contains flavonoids and phenolic compounds which have iron chelating and antioxidant property. Hence, present study was designed to investigate the beneficial effects of different fractions ofM. officinalisfor the management of iron overload disease and its complications. Iron overload was induced by 6 IP injections of iron dextran (12.5 mg/100 g) uniformly distributed over the period of 30 days. The different fractions ofM. officinaliswere given orally and Deferoxamine (DFO) subcutaneously for 30 days. The iron chelating and various biochemical parameters were estimated on 15thand 30thday. The different fractions ofM. officinalisdemonstrated dose dependant in-vitro iron chelating ability. There were significant (P<0.01) iron chelating potential shows in rats treated with methanolic fraction of methanolic extract (MFME) and methanolic fraction of aqueous extract (MFAE) ofM. officinalisas compared to disease control (DC) rats. The rats treated with MFME and MFAE ofM. officinalisshows significant (P<0.01) antioxidant and vital organ protective effect as compared to DC rats. Better iron chelation was observed on 30thday and at higher dose (300 mg/kg) as compared to 15thday and at lower dose (150 mg/kg). The present study concludes that MFME and MFAE ofM. officinalishave reversible iron chelating and antioxidant potential in rats. The study also proves the possible mechanism of action, as an iron chelator by increasing the excretion of iron in urine and feces.
Department of Pharmacology, Smt. C. V. Gajera Pharmacy Mahila College, Amreli, Gujarat, India.
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NA
Sheikh
Department of Pharmacology, School of Pharmacy, RK University, Rajkot, Gujarat, India.
T R
TR
Desai
Department of Pharmacology, School of Pharmacy, RK University, Rajkot, Gujarat, India.
P R
PR
Tirgar
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000502:physiology; D003676:Deferoxamine / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D007501:Iron; D007502:Iron Chelating Agents / Q000494:pharmacology*; D019190:Iron Overload / Q000188:drug therapy*; D007505:Iron-Dextran Complex / Q000494:pharmacology*; D008297:Male; D029903:Melilotus / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D003911:Dextrans; D005609:Free Radicals; D007502:Iron Chelating Agents; D007505:Iron-Dextran Complex; D007501:Iron; D003676:Deferoxamine