Interface-free capillary electrophoresis-mass spectrometry system with nanospray ionization-Analysis of dexrazoxane in blood plasma.
The newly developed interface-free capillary electrophoresis-nanospray/mass spectrometry system (CE-nESI/MS) was applied for rapid analysis of the cardioprotective drug dexrazoxane and its hydrolysed form ADR-925 in deproteinized blood plasma samples. The aim of this study was to test the simplest possible CE-nESI/MS instrumentation for analyses of real samples. This interface-free system, utilizing single piece of a narrow bore capillary as both the electrophoretic separation column and the nanospray emitter, was operated at a flow rate of 30nL/min. Excellent electrophoretic separation and sensitive nanospray ionization was achieved with the use of only one high voltage power supply. In addition, hydrophobic external coating was developed and tested for additional stability of the nanospray ionization. To our knowledge this is the first study devoted to the analysis of dexrazoxane and ADR-925 by capillary electrophoresis-mass spectrometry.
Institute of Analytical Chemistry of the CAS, v. v. i., Veveri 97, Brno, 602 00, Czech Republic; Faculty of Science, Masaryk University, Kotlarska 2, Brno, 611 37, Czech Republic.
Anna
A
Tycova
Faculty of Science, Masaryk University, Kotlarska 2, Brno, 611 37, Czech Republic.
Marek
M
Vido
Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, Heyrovskeho 1203, Hradec Kralove, 500 05, Czech Republic.
Petra
P
Kovarikova
Institute of Analytical Chemistry of the CAS, v. v. i., Veveri 97, Brno, 602 00, Czech Republic; CEITEC - Central European Institute of Technology, Kamenice 753/5, Brno, 625 00, Czech Republic. Electronic address: foret@iach.cz.
Frantisek
F
Foret
D001774:Blood Chemical Analysis / Q000379:methods*; D064730:Dexrazoxane / Q000097:blood*; D019075:Electrophoresis, Capillary; D005029:Ethylenediamines / Q000097:blood*; D005998:Glycine / Q000031:analogs & derivatives* / Q000097:blood; D021241:Spectrometry, Mass, Electrospray Ionization
D005029:Ethylenediamines; D064730:Dexrazoxane; C059866:ICRF 198; D005998:Glycine
Capillary electrophoresis; Dexrazoxane; Hydrophobic coating; Interface; Mass spectrometry; Nanospray