Three-dimensional pancreas organogenesis models.
A rediscovery of three-dimensional culture has led to the development of organ biogenesis, homeostasis and disease models applicable to human tissues. The so-called organoids that have recently flourished serve as valuable models bridging between cell lines or primary cells grown on the bottom of culture plates and experiments performed in vivo. Though not recapitulating all aspects of organ physiology, the miniature organs generated in a dish are useful models emerging for the pancreas, starting from embryonic progenitors, adult cells, tumour cells and stem cells. This review focusses on the currently available systems and their relevance to the study of the pancreas, of β-cells and of several pancreatic diseases including diabetes. We discuss the expected future developments for studying human pancreas development and function, for developing diabetes models and for producing therapeutic cells.
DanStem, University of Copenhagen, Copenhagen, Denmark. anne.grapin-botton@sund.ku.dk.
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Grapin-Botton
D000818:Animals; D006801:Humans; D050417:Insulin-Secreting Cells; D051379:Mice; D009924:Organ Culture Techniques; D038081:Organogenesis; D009940:Organoids; D010179:Pancreas / Q000166:cytology / Q000196:embryology* / Q000254:growth & development; D010190:Pancreatic Neoplasms
bioengineering; diabetes; disease; modelling; organoids; spheres; spheroids; stem cells; therapy