[Knockdown of dachshund homolog 1 (DACH1) promotes cell apoptosis and inhibits the invasion and migration abilities of Capan-1 pancreatic cancer cells].
Objective To investigate the impact of the decreased expression of dachshund homolog 1 (DACH1) on cell cycle, apoptosis, invasion and migration of Capan-1 pancreatic cancer cells. Methods After four pairs of DACH1 siRNA were designed and synthesized, double-stranded short hairpin RNA (shRNA) were annealed and inserted into pGenesil-1 vector. The product was then confirmed by enzyme digestion and sequencing analysis. The recombinant plasmids were transfected into Capan-1 cells via Lipofectamine(TM) 2000. Fluorescence microscopy, reverse transcription PCR (RT-PCR) and Western blotting were used to detect the transfection efficiency. Cell apoptosis and cell cycle were tested by flow cytometry. Transwell(TM) assay was used to monitor the invasion and migration abilities of Capan-1 cells. Results Recombinant plasmid pshRNA-DACH1 was successfully constructed and transfected into Capan-1 cells. After transfection, the expression of DACH1 was reduced to some extent. Flow cytometry revealed that cell apoptosis was promoted in the pshRNA-DACH1 transfected group compared with control groups, whereas cell cycle had no significant differences among the groups. Transwell(TM) assay validated that the abilities of migration and invasion were inhibited in the pshRNA-DACH1 transfected group. Conclusion Knockdown of DACH1 expression can remarkably enhance the cell apoptosis, restrain the proliferation, migration and invasion of Capan-1 cells.
Department of Gastroenterology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Xiaona
X
Bu
Department of Gastroenterology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Chuan
C
Wang
Department of Gastroenterology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. *Corresponding author, E-mail: jiangz1753@163.com.
Zheng
Z
Jiang
D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006801:Humans; D009361:Neoplasm Invasiveness; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
C486747:DACH1 protein, human; D005136:Eye Proteins; D034741:RNA, Small Interfering; D014157:Transcription Factors