title: Renovascular hypertension: Effects of mesenchymal stem cells in the contralateral hypertensive kidney in rats.
abstract: Mesenchymal stem cells (MSC) induced neovascularization and improved renal morphology of the stenotic kidney in 2 kidneys-1 clip (2K-1C) model of renovascular hypertension. The present study evaluated the effects of MSC in the contralateral hypertensive kidney. Three weeks after left renal artery occlusion, MSC were injected into the tail vein of the 2K-1C rats. Renal function and morphology were analyzed in both kidneys. Labeled MSC were found in stenotic and contralateral kidneys. Hypertensive 2K-1C animals presented increased circulating levels of Angiotensin II (Ang II) and renin. MSC prevented the progressive increase of blood pressure and reduced circulating Ang II and renin levels. Stenotic kidney showed reduced renal plasma flow (RPF) and glomerular filtration rate (GFR), whereas the contralateral kidney had a tendency (p > 0.5) of reduction in GFR in spite of unchanged RPF. MSC treatment caused an improvement in GFR with no effect of on RPF in the stenotic kidney. Contralateral kidney showed increased diuresis and natriuresis that were even higher in MSC-treated animals, indicating that cell treatment improved the capacity of the contralateral kidney to excrete sodium. Contralateral kidney expressed higher levels of inflammatory cytokines (IL-6, TNF-α) and signs of fibrosis, which were attenuated by MSC treatment. MSC treatment improved the stenotic kidney function, and it was also beneficial to the contralateral hypertensive kidney because it improved the morphology and preserved its capacity to excrete sodium.
authors.affiliation: a Departament of Medicine, Renal Division , Federal University of Sao Paulo , Sao Paulo , Brazil.
authors.forename: Elizabeth Barbosa de
authors.initials: EB
authors.lastname: Oliveira-Sales
authors.affiliation: a Departament of Medicine, Renal Division , Federal University of Sao Paulo , Sao Paulo , Brazil.
authors.forename: Vanessa Araujo
authors.initials: VA
authors.lastname: Varela
authors.affiliation: a Departament of Medicine, Renal Division , Federal University of Sao Paulo , Sao Paulo , Brazil.
authors.forename: Edgar
authors.initials: E
authors.lastname: Maquigussa
authors.affiliation: a Departament of Medicine, Renal Division , Federal University of Sao Paulo , Sao Paulo , Brazil.
authors.forename: Fernanda Teixeira
authors.initials: FT
authors.lastname: Borges
authors.affiliation: b Department of Physiology , Federal University of Sao Paulo , Sao Paulo , Brazil.
authors.forename: Caroline Gusson
authors.initials: CG
authors.lastname: Shimoura
authors.affiliation: b Department of Physiology , Federal University of Sao Paulo , Sao Paulo , Brazil.
authors.forename: Guiomar
authors.initials: G
authors.lastname: Gomes
authors.affiliation: b Department of Physiology , Federal University of Sao Paulo , Sao Paulo , Brazil.
authors.forename: Ruy Ribeiro
authors.initials: RR
authors.lastname: Campos
authors.affiliation: a Departament of Medicine, Renal Division , Federal University of Sao Paulo , Sao Paulo , Brazil.
authors.forename: Mirian Aparecida
authors.initials: MA
authors.lastname: Boim
mesh_terms: D000804:Angiotensin II / Q000097:blood*; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D006978:Hypertension, Renovascular / Q000503:physiopathology / Q000517:prevention & control; D015850:Interleukin-6 / Q000378:metabolism; D007668:Kidney / Q000473:pathology / Q000503:physiopathology; D007677:Kidney Function Tests / Q000379:methods; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051381:Rats; D012077:Renal Artery / Q000601:surgery; D065667:Renal Elimination / Q000502:physiology; D012083:Renin / Q000097:blood*; D012964:Sodium / Q000378:metabolism; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D000804:Angiotensin II; D012964:Sodium; D012083:Renin
keywords: Mesenchymal stem cells; renal function; renin–angiotensin system; renovascular hypertension