title: Maternal high fructose and low protein consumption during pregnancy and lactation share some but not all effects on early-life growth and metabolic programming of rat offspring.
abstract: Maternal nutritional stress during pregnancy acts to program offspring metabolism. We hypothesized that the nutritional stress caused by maternal fructose or low protein intake during pregnancy would program the offspring to develop metabolic aberrations that would be exacerbated by a diet rich in fructose or fat during adult life. The objective of this study was to characterize and compare the fetal programming effects of maternal fructose with the established programming model of a low-protein diet on offspring. Male offspring from Sprague-Dawley dams fed a 60% starch control diet, a 60% fructose diet, or a low-protein diet throughout pregnancy and lactation were weaned onto either a 60% starch control diet, 60% fructose diet, or a 30% fat diet for 15 weeks. Offspring from low-protein and fructose-fed dam showed retarded growth (P<.05) at weaning (50.3, 29.6 vs 59.1±0.8 g) and at 18 weeks of age (420, 369 vs 464±10.9 g). At 18 weeks of age, offspring from fructose dams expressed greater quantities (P<.05) of intestinal Pgc1a messenger RNA compared with offspring from control or low-protein dams (1.31 vs 0.89, 0.85; confidence interval, 0.78-1.04). Similarly, maternal fructose (P=.09) and low-protein (P<.05) consumption increased expression of Pgc1a in offspring liver (7.24, 2.22 vs 1.22; confidence interval, 2.11-3.45). These data indicate that maternal fructose feeding is a programming model that shares some features of maternal protein restriction such as retarded growth, but is unique in programming of selected hepatic and intestinal transcripts.
authors.affiliation: Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN, USA.
authors.forename: Emily J
authors.initials: EJ
authors.lastname: Arentson-Lantz
authors.affiliation: Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN, USA.
authors.forename: Mi
authors.initials: M
authors.lastname: Zou
authors.affiliation: Department of Nutrition Science, Purdue University, West Lafayette, IN, USA.
authors.forename: Dorothy
authors.initials: D
authors.lastname: Teegarden
authors.affiliation: Department of Nutrition Science, Purdue University, West Lafayette, IN, USA.
authors.forename: Kimberly K
authors.initials: KK
authors.lastname: Buhman
authors.affiliation: Department of Animal Science, Purdue University, West Lafayette, IN, USA. Electronic address: sdonkin@purdue.edu.
authors.forename: Shawn S
authors.initials: SS
authors.lastname: Donkin
mesh_terms: D000818:Animals; D001835:Body Weight; D001942:Breast Feeding; D004032:Diet; D018753:Diet, Protein-Restricted / Q000009:adverse effects; D004044:Dietary Proteins / Q000008:administration & dosage; D000073417:Dietary Sugars / Q000008:administration & dosage / Q000009:adverse effects; D005247:Feeding Behavior; D005260:Female; D047109:Fetal Development; D005632:Fructose / Q000009:adverse effects*; D006130:Growth Disorders / Q000209:etiology* / Q000378:metabolism; D007774:Lactation / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism; D011488:Protein Deficiency / Q000150:complications / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D014886:Weaning
chemical_list: D004044:Dietary Proteins; D000073417:Dietary Sugars; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C501429:Ppargc1a protein, rat; D012333:RNA, Messenger; D005632:Fructose
keywords: Fetal programming; Fructose; Liver; Low protein; Rat