title: Model Lung Surfactant Films: Why Composition Matters.
abstract: Lung surfactant replacement therapies, Survanta and Infasurf, and two lipid-only systems both containing saturated and unsaturated phospholipids and one containing additional palmitic acid were used to study the impact of buffered saline on the surface activity, morphology, rheology, and structure of Langmuir monolayer model membranes. Isotherms and Brewster angle microscopy show that buffered saline subphases induce a film expansion, except when the cationic protein, SP-B, is present in sufficient quantities to already screen electrostatic repulsion, thus limiting the effect of changing pH and adding counterions. Grazing incidence X-ray diffraction results indicate an expansion not only of the liquid expanded phase but also an expansion of the lattice of the condensed phase. The film expansion corresponded in all cases with a significant reduction in the viscosity and elasticity of the films. The viscoelastic parameters are dominated by liquid expanded phase properties and do not appear to be dependent on the structure of the condensed phase domains in a phase separated film. The results highlight that the choice of subphase and film composition is important for meaningful interpretations of measurements using model systems.
authors.affiliation: Department of Chemistry and Biochemistry and Centre for NanoScience Research, Concordia University , 7141 Sherbrooke Street West, Montreal, Canada H4B 1R6.
authors.forename: Sahana L
authors.initials: SL
authors.lastname: Selladurai
authors.affiliation: Department of Chemistry and Biochemistry and Centre for NanoScience Research, Concordia University , 7141 Sherbrooke Street West, Montreal, Canada H4B 1R6.
authors.forename: Renaud
authors.initials: R
authors.lastname: Miclette Lamarche
authors.affiliation: Department of Chemistry and Biochemistry and Centre for NanoScience Research, Concordia University , 7141 Sherbrooke Street West, Montreal, Canada H4B 1R6.
authors.forename: Rolf
authors.initials: R
authors.lastname: Schmidt
authors.affiliation: Department of Chemistry and Biochemistry and Centre for NanoScience Research, Concordia University , 7141 Sherbrooke Street West, Montreal, Canada H4B 1R6.
authors.forename: Christine E
authors.initials: CE
authors.lastname: DeWolf