title: High butter-fat diet and bisphenol A additively impair male rat spermatogenesis.
abstract: Exposure to xenoestrogens is a probable cause of male infertility in humans. Consumption of high-fat diets and exposure to bisphenol A (BPA) is pervasive in America. Here, we test the hypothesis that gestational exposure to high dietary fats and/or BPA disrupt spermatogenesis in adulthood. Sprague-Dawley rats were fed diets containing 10kcal% butter fat (AIN), 39kcal% butter fat (HFB), or 39kcal% olive oil (HFO), with or without BPA (25μg/kg body weight/day) during pregnancy. One group of male offspring received testosterone (T)- and estradiol-17β (E2)-filled implants or sham-implants from postnatal day (PND)70-210. Another group was naturally aged to 18 months. We found that adult males with gestational exposure to BPA, HFB, or HFB+BPA, in both the aged group and the T+E2-implanted group, exhibited impairment of spermatogenesis. In contrast, gestational exposure to HFO or HFO+BPA did not affect spermatogenesis. Sham-implanted, gestational exposed groups also had normal spermatogenesis. Loss of ERα expression in round spermatids and premature expression of protamine-1 in diplotene spermatocytes were features associated with impaired spermatogenesis. Compared with the single-treatment groups, the HFB+BPA group experienced more severe effects, including atrophy.
authors.affiliation: Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, OH, USA; Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, OH, USA; Cincinnati Cancer Center, Cincinnati, OH, USA. Electronic address: Pheruza.tarapore@uc.edu.
authors.forename: Pheruza
authors.initials: P
authors.lastname: Tarapore
authors.affiliation: Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, OH, USA.
authors.forename: Max
authors.initials: M
authors.lastname: Hennessy
authors.affiliation: Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, OH, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Song
authors.affiliation: Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, OH, USA; Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, OH, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Ying
authors.affiliation: Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, OH, USA; Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, OH, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Ouyang
authors.affiliation: Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, OH, USA; Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, OH, USA.
authors.forename: Vinothini
authors.initials: V
authors.lastname: Govindarajah
authors.affiliation: Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, OH, USA; Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, OH, USA; Cincinnati Cancer Center, Cincinnati, OH, USA.
authors.forename: Yuet-Kin
authors.initials: YK
authors.lastname: Leung
authors.affiliation: Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, OH, USA; Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, OH, USA; Cincinnati Cancer Center, Cincinnati, OH, USA; Cincinnati Veteran Affairs Hospital Medical Center, Cincinnati, OH, USA. Electronic address: shuk-mei.ho@uc.edu.
authors.forename: Shuk-Mei
authors.initials: SM
authors.lastname: Ho
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D002079:Butter; D059305:Diet, High-Fat / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D000069463:Olive Oil; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced* / Q000503:physiopathology; D017207:Rats, Sprague-Dawley; D013091:Spermatogenesis / Q000187:drug effects*; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D002079:Butter; D004785:Environmental Pollutants; D004958:Estradiol; D000069463:Olive Oil; D010636:Phenols; D013739:Testosterone; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Endocrine disrupting chemicals; High fat butter; High fat olive oil; Testis