title: 8-Prenylnaringenin promotes recovery from immobilization-induced disuse muscle atrophy through activation of the Akt phosphorylation pathway in mice.
abstract: 8-Prenylnaringenin (8-PN) is a prenylflavonoid that originates from hop extracts and is thought to help prevent disuse muscle atrophy. We hypothesized that 8-PN affects muscle plasticity by promoting muscle recovery under disuse muscle atrophy. To test the promoting effect of 8-PN on muscle recovery, we administered an 8-PN mixed diet to mice that had been immobilized with a cast to one leg for 14 days. Intake of the 8-PN mixed diet accelerated recovery from muscle atrophy, and prevented reductions in Akt phosphorylation. Studies on cell cultures of mouse myotubes in vitro demonstrated that 8-PN activated the PI3K/Akt/P70S6K1 pathway at physiological concentrations. A cell-culture study using an inhibitor of estrogen receptors and an in vivo experiment with ovariectomized mice suggested that the estrogenic activity of 8-PN contributed to recovery from disuse muscle atrophy through activation of an Akt phosphorylation pathway. These data strongly suggest that 8-PN is a naturally occurring compound that could be used as a nutritional supplement to aid recovery from disuse muscle atrophy.
authors.affiliation: Department of Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan; rmukai@tokushima-u.ac.jp.
authors.forename: Rie
authors.initials: R
authors.lastname: Mukai
authors.affiliation: Department of Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Horikawa
authors.affiliation: Department of Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: Pei-Yi
authors.initials: PY
authors.lastname: Lin
authors.affiliation: Department of Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Tsukumo
authors.affiliation: Department of Nutritional Physiology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan; and.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nikawa
authors.affiliation: Department of Pharmaceutical Chemistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kawamura
authors.affiliation: Department of Pharmaceutical Chemistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Nemoto
authors.affiliation: Department of Food Science, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Terao
mesh_terms: D000818:Animals; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D044950:Flavanones / Q000008:administration & dosage / Q000494:pharmacology*; D019417:Hindlimb Suspension; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects / Q000503:physiopathology*; D009133:Muscular Atrophy / Q000188:drug therapy / Q000503:physiopathology*; D051058:Oncogene Protein v-akt / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D048789:Phytoestrogens / Q000008:administration & dosage / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects; D016896:Treatment Outcome
chemical_list: C119737:8-prenylnaringenin; D044950:Flavanones; D009124:Muscle Proteins; D048789:Phytoestrogens; D051058:Oncogene Protein v-akt
keywords: 8-prenylnaringenin; IGF1/PI3K/Akt pathway; disuse muscle atrophy; nutritional supplement; phosphorylation