title: Construction of novel electrochemical immunosensor for detection of prostate specific antigen using ferrocene-PAMAM dendrimers.
abstract: In this study, an immunosensor was designed to utilize for the detection of prostate specific antigen (PSA) based on three different generations (G1, G2 and G3) of ferrocene (Fc) cored polyamidiamine dendrimers (Fc-PAMAM) gold (Au) electrode. The self-assembled monolayer principle (SAM) was used to fabricate the sensitive, selective and disposable immunosensor electrodes. In electrode fabrication cysteamine (Cys) was the first agent covalently linked on the Au electrode surface. Immobilized redox center (ferrocene) cored PAMAM dendrimers served as a layer for the further binding of biological components. The monoclonal antibody of PSA (anti-PSA) was covalently immobilized on dendrimers which were attached onto the modified Au surface (Au/Cys/Fc-PAMAMs/anti-PSA). PSA levels were quantitatively analyzed by using electrochemical differential pulse voltammetry (DPV) whose lowest detection limit was calculated as 0.001ngmL(-1). The Au/Cys/FcPAMAM/anti-PSA immunosensor showed excellent performance for PSA at the pulse amplitude; 50mV and the scan rate; 10mV/s in a wide linear concentration range of 0.01ng-100ngmL(-1). Analytical performance and specificity assays were carried out using human serum and different proteins.
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey. Electronic address: ecevik@fatih.edu.tr.
authors.forename: Emre
authors.initials: E
authors.lastname: Çevik
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey.
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Bahar
authors.affiliation: Institute of Biomedical Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Şenel
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey.
authors.forename: M Fatih
authors.initials: MF
authors.lastname: Abasıyanık
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D003217:Conductometry / Q000295:instrumentation*; D050091:Dendrimers / Q000737:chemistry*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005296:Ferrous Compounds / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D008297:Male; D000075163:Metallocenes; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D050091:Dendrimers; D005296:Ferrous Compounds; D000075163:Metallocenes; C104700:PAMAM Starburst; D017430:Prostate-Specific Antigen; C004998:ferrocene
keywords: Dendrimer; Ferrocene; Immunosensor; Prostate specific antigen