title: Protective Role of Corilagin on Aβ25-35-Induced Neurotoxicity: Suppression of NF-κB Signaling Pathway.
abstract: Aggregation and deposition of beta-amyloid peptides (Aβ), a pathological hallmark of Alzheimer's disease, has been recognized as a potent activator of neuroinflammation and neuronal dysfunction. In this study, the underlying molecular mechanisms responsible for the neuroprotective effects of corilagin against Aβ25-35-triggered neurotoxicity and inflammatory responses were investigated in PC12 cells. Pretreatment with corilagin effectively protected PC12 cells against Aβ25-35-induced damage and apoptosis. Aβ25-35induced damage in PC12 cells as revealed by increased production of reactive oxygen species, caspase-3 activity, and cell cycle arrest was attenuated by corilagin pretreatment. Corilagin not only significantly suppressed the production of neurotoxic inflammatory mediators such as tumor necrosis factor-α, nitric oxide, and prostaglandin E2but also downregulated cyclooxygenase-2 and inducible nitric oxide synthase expression in PC12 cells. It also exerted a beneficial effect by suppressing the degradation of inhibitor of κB (IκB)-α and subsequent activation of transcription factor nuclear factor κB (NF-κB), mostly through inhibition of extracellular signal-regulated kinase activity in comparison to c-Jun N-terminal kinase and p38 MAP kinase (p38) mitogen-activated protein kinase activity. These findings suggest that attenuation of Aβ25-35-induced inflammatory responses by downregulating the NF-κB signaling pathway might be a valuable strategy for both Alzheimer's disease prevention and/or treatment.
authors.affiliation: 1 Department of Food Science and Nutrition, Dong-A University , Busan, Korea.
authors.forename: Kumju
authors.initials: K
authors.lastname: Youn
authors.affiliation: 1 Department of Food Science and Nutrition, Dong-A University , Busan, Korea.
authors.forename: Seonah
authors.initials: S
authors.lastname: Lee
authors.affiliation: 2 Department of Food and Life Science, College of Biomedical Science & Engineering, Inje University , Gimhae, Korea.
authors.forename: Woo-Sik
authors.initials: WS
authors.lastname: Jeong
authors.affiliation: 3 Department of Food Science, Rutgers University , New Brunswick, New Jersey, USA .
authors.forename: Chi-Tang
authors.initials: CT
authors.lastname: Ho
authors.affiliation: 1 Department of Food Science and Nutrition, Dong-A University , Busan, Korea.|4 Institute of Convergence Bio-Health (ICBH), Dong-A University , Busan, Korea.
authors.forename: Mira
authors.initials: M
authors.lastname: Jun