title: Optimization of the Production of Extracellular Polysaccharide from the Shiitake Medicinal Mushroom Lentinus edodes (Agaricomycetes) Using Mutation and a Genetic Algorithm-Coupled Artificial Neural Network (GA-ANN).
abstract: Exopolysaccharide (EPS) production by a strain of Lentinus edodes was studied via the effects of treatments with ultraviolet (UV) irradiation and acridine orange. Furthermore, optimization of EPS production was studied using a genetic algorithm coupled with an artificial neural network in submerged fermentation. Exposure to irradiation and acridine orange resulted in improved EPS production (2.783 and 5.548 g/L, respectively) when compared with the wild strain (1.044 g/L), whereas optimization led to improved productivity (23.21 g/L). The EPS produced by various strains also demonstrated good DPPH scavenging activities of 45.40-88.90%, and also inhibited the growth of Escherichia coli and Klebsiella pneumoniae. This study shows that multistep optimization schemes involving physical-chemical mutation and media optimization can be an attractive strategy for improving the yield of bioactives from medicinal mushrooms. To the best of our knowledge, this report presents the first reference of a multistep approach to optimizing EPS production in L. edodes.
authors.affiliation: Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
authors.forename: Adeyemi Ojutalayo
authors.initials: AO
authors.lastname: Adeeyo
authors.affiliation: Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
authors.forename: Agbaje
authors.initials: A
authors.lastname: Lateef
authors.affiliation: Department of Microbiology, School of of Life Sciences, University of KwaZulu-Natal, Scottsville, Pietermaritzburg, South Africa.
authors.forename: Evariste Bosco
authors.initials: EB
authors.lastname: Gueguim-Kana
mesh_terms: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D009154:Mutation; D016571:Neural Networks, Computer; D010851:Picrates; D020942:Shiitake Mushrooms / Q000235:genetics / Q000378:metabolism*
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D062610:Fungal Polysaccharides; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl