title: Identification and Characterization of SUMO-SIM Interactions.
abstract: The covalent attachment of SUMO to lysine residues of cellular proteins serves as an important mechanism for the dynamic control of protein networks. SUMO conjugates typically mediate selected protein-protein interactions by binding to specific recognition modules. Identification of SUMO-binding proteins and the characterization of the binding motifs are key to understanding SUMO signaling. Here we describe two complementary approaches that are used to tackle these questions.
authors.affiliation: Institute of Biochemistry II, Goethe University, Medical School, Theodor-Stern-Kai 7, 60590, Frankfurt (Main), Germany. K.Husnjak@biochem2.uni-frankfurt.de.
authors.forename: Koraljka
authors.initials: K
authors.lastname: Husnjak
authors.affiliation: Institute of Biochemistry II, Goethe University, Medical School, Theodor-Stern-Kai 7, 60590, Frankfurt (Main), Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Keiten-Schmitz
authors.affiliation: Institute of Biochemistry II, Goethe University, Medical School, Theodor-Stern-Kai 7, 60590, Frankfurt (Main), Germany. Ste.Mueller@em.uni-frankfurt.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Müller
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping / Q000379:methods*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D025841:Small Ubiquitin-Related Modifier Proteins / Q000235:genetics / Q000378:metabolism*; D058207:Sumoylation; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D025842:SUMO-1 Protein; C495501:SUMO1 protein, human; C117909:SUMO2 protein, human; C492189:SUMO3 protein, human; D025841:Small Ubiquitin-Related Modifier Proteins; D014452:Ubiquitins
keywords: Protein interaction; SUMO; SUMO-interacting motif; Yeast two-hybrid