title: Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.
abstract: The organization of nuclear domains is crucial for biological events including virus infection. Newly synthesized influenza viral genome forms viral ribonucleoprotein (vRNP) complexes and is exported from the nucleus to the cytoplasm through a CRM1-dependent pathway mediated by viral proteins M1 and NS2. However, the spatio-temporal regulation of the progeny vRNP in the nucleus is still unclear. Here we found that polycomb repressive complex 2 (PRC2), which contains a methyltransferase subunit EZH2 and catalyzes histone H3K27me3 for the formation of facultative heterochromatin, is a positive factor for the virus production. Depletion of PRC2 complex showed the nuclear accumulation of vRNP and the reduction of M1-vRNP complex formation. We also found that PRC2 complex directly binds to M1, and facilitates the interaction of M1 with vRNP. In conclusion, we propose that the progeny vRNP could be recruited to facultative heterochromatin and assembled into the export complex mediated by PRC2 complex.
authors.affiliation: Department of Infection Biology, Faculty of Medicine and Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Masamitsu N
authors.initials: MN
authors.lastname: Asaka
authors.affiliation: Department of Infection Biology, Faculty of Medicine and Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.|Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kawaguchi
authors.affiliation: Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Sakai
authors.affiliation: Department of Infection Biology, Faculty of Medicine and Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Mori
authors.affiliation: Department of Infection Biology, Faculty of Medicine and Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Nagata
mesh_terms: D021581:Active Transport, Cell Nucleus; D002460:Cell Line; D016679:Genome, Viral; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D007251:Influenza, Human / Q000378:metabolism* / Q000821:virology*; D063151:Polycomb Repressive Complex 2 / Q000378:metabolism*; D011485:Protein Binding; D034443:RNA Transport; D012367:RNA, Viral; D014763:Viral Matrix Proteins / Q000378:metabolism; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D014763:Viral Matrix Proteins; D063151:Polycomb Repressive Complex 2