title: Comparative study of adipose-derived stem cells and bone marrow-derived stem cells in similar microenvironmental conditions.
abstract: Mesenchymal stem cells (MSCs), which were first isolated from the bone marrow, are now being extracted from various other tissues in the body, including the adipose tissue. The current study presents systematic evidence of how the adipose tissue-derived stem cells (ASCs) and bone marrow-derived mesenchymal stem cells (Bm-MSCs) behave when cultured in specific pro-adipogenic microenvironments. The cells were first characterized and identified as MSCs in terms of their morphology, phenotypic expression, self-renewal capabilities and multi-lineage potential. Subsequently, the proliferation and gene expression profiles of the cell populations cultured on two-dimensional (2D) adipose tissue extracellular matrix (ECM)-coated tissue culture plastic (TCP) and in three-dimensional (3D) AlgiMatrix® microenvironments were analyzed. Overall, it was found that adipogenesis was triggered in both cell populations due to the presence of adipose tissue ECM. However, in 3D microenvironments, ASCs and Bm-MSCs were predisposed to the adipogenic and osteogenic lineages respectively. Overall, findings from this study will contribute to ongoing efforts in adipose tissue engineering as well as provide new insights into the role of the ECM and cues provided by the immediate microenvironment for stem cell differentiation.
authors.affiliation: Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
authors.forename: Vipra
authors.initials: V
authors.lastname: Guneta
authors.affiliation: School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore; KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore 229899, Singapore; Institute of Molecular and Cell Biology, Agency for Science Technology & Research (A⁎STAR), 61 Biopolis Drive, Proteos, Singapore 138673, Singapore.
authors.forename: Nguan Soon
authors.initials: NS
authors.lastname: Tan
authors.affiliation: School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Soon Kiat Jeremy
authors.initials: SK
authors.lastname: Chan
authors.affiliation: Bioinformatics Institute, Agency for Science Technology & Research (A⁎STAR), 30 Biopolis Street, Matrix, Singapore 138671, Singapore.
authors.forename: Vivek
authors.initials: V
authors.lastname: Tanavde
authors.affiliation: Division of Plastic, Reconstructive and Aesthetic Surgery, Department of Surgery, National University Hospital (NUH) and National University of Singapore (NUS), Kent Ridge Wing, Singapore 119074, Singapore.
authors.forename: Thiam Chye
authors.initials: TC
authors.lastname: Lim
authors.affiliation: Plastic, Reconstructive and Aesthetic Surgery Section, Tan Tock Seng Hospital (TTSH), 11, Jalan Tan Tock Seng, Singapore 308433, Singapore.
authors.forename: Thien Chong Marcus
authors.initials: TC
authors.lastname: Wong
authors.affiliation: Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore; KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore 229899, Singapore. Electronic address: cleochoong@ntu.edu.sg.
authors.forename: Cleo
authors.initials: C
authors.lastname: Choong
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology*; D048430:Cell Shape / Q000235:genetics; D002478:Cells, Cultured; D060833:Cellular Microenvironment / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis
keywords: Adipose-derived stem cells (ASCs); Bone-marrow derived stem cells (Bm-MSCs); Cell-ECM interaction; Microenvironment; Stem cell differentiation