title: Telocytes in gastric lamina propria of the Chinese giant salamander, Andrias davidianus.
abstract: In this study, we attempt to identify gastric telocytes (TCs) of the Chinese giant salamander Andrias davidianus, by light microscopy, immunohistochemistry and transmission electron microscopy (TEM) methods. Toluidine blue staining showed TCs with one to two very thin and long telopodes (Tps) that were located in gastric lamina propria. Tps had characteristic structures, including podoms, podomers and dichotomous branching. Immunohistochemistry showed the existence of CD34(+)/PDGFRα(+) TCs with moniliform Tps in stroma and were close to gastric glands and blood vessels. TEM micrographs also demonstrated the presence of TCs in interstitium between gastric glands. TCs/Tps were located in close proximity to gastric glands, blood vessels, endocrine cells and stem cells. In particular, Tps frequently surrounded stem cells. TCs and Tps, Tps and stem cells established close contacts. Moreover, the exosomes were also found near TCs/Tps. Our data confirmed the presence of TCs in gastric lamina propria of the amphibian, and suggested that TCs cooperate with resident stem cells to regulate endocrine cells and gastric glands regeneration and homeostasis.
authors.affiliation: College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
authors.forename: Shengwei
authors.initials: S
authors.lastname: Zhong
authors.affiliation: College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Yu
authors.affiliation: College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Ge
authors.affiliation: College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Peng
authors.affiliation: College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
authors.forename: Zuohong
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D005753:Gastric Mucosa / Q000166:cytology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism; D000067170:Telocytes / Q000166:cytology* / Q000648:ultrastructure; D014562:Caudata / Q000033:anatomy & histology*
chemical_list: D018952:Antigens, CD34; D020796:Receptor, Platelet-Derived Growth Factor alpha