title: Decreased n-6/n-3 polyunsaturated fatty acid ratio reduces chronic reflux esophagitis in rats.
abstract: To investigate the effect of dietary ratio of n-6/n-3 PUFAs on chronic reflux esophagitis (RE) and lipid peroxidation.
  
  
  
  Rat RE model were established and then fed on a diet contained different n-6/n-3 PUFA ratios (1:1.5, 5:1, 10:1) or received pure n-6 PUFA diet for 14 days. Esophageal pathological changes were evaluated using macroscopic examination and hematoxyline-eosin staining. IL-1β, IL-8, and TNFα mRNA and protein levels of were determined using RT-PCR and Western blotting, respectively. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined using ELISA.
  
  
  
  The severity of esophagitis was lowest in the PUFA(1:1.5) group (P<0.05). IL-1β, IL-8, and TNFα mRNA and protein and MDA levels were significantly increased in model groups with the increasing n-6/n-3 PUFA ratios. SOD levels were significantly decreased in all RE PUFA groups (P<0.05).
  
  
  
  Esophageal injury and lipid peroxidation appeared to be ameliorated by increased n-3 PUFAs intake.
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of Fujian Medical University, China. Electronic address: weijingjing0208@163.com.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Wei
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of Fujian Medical University, China. Electronic address: tangdupeng1989@163.com.
authors.forename: Du-Peng
authors.initials: DP
authors.lastname: Tang
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of Fujian Medical University, China. Electronic address: 328230221@qq.com.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xie
authors.affiliation: Department of Endocrinology, the First Affiliated Hospital of Fujian Medical University, China. Electronic address: yly_lm@sina.com.
authors.forename: Li-Yong
authors.initials: LY
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital of Fujian Medical University, China. Electronic address: zhuang203@yeah.net.
authors.forename: Ze-Hao
authors.initials: ZH
authors.lastname: Zhuang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000494:pharmacology; D004041:Dietary Fats / Q000008:administration & dosage* / Q000494:pharmacology; D004195:Disease Models, Animal; D004942:Esophagitis, Peptic / Q000178:diet therapy* / Q000235:genetics / Q000276:immunology; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage* / Q000494:pharmacology; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; C000594089:IL1B protein, rat; D053583:Interleukin-1beta; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D013482:Superoxide Dismutase
keywords: Dietary fat; Inflammation; Lipid peroxidation; Polyunsaturated fatty acid; Reflux esophagitis