title: GADD45β, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens.
abstract: Avian leukosis virus subgroup J (ALV-J) is a retroviruses that induces neoplasia, hepatomegaly, immunosuppression and poor performance in chickens. The tumorigenic and pathogenic mechanisms of ALV-J remain a hot topic. To explore anti-tumor genes that promote resistance to ALV-J infection in chickens, we bred ALV-J resistant and susceptible chickens (F3 generation). RNA-sequencing (RNA-Seq) of liver tissue from the ALV-J resistant and susceptible chickens identified 216 differentially expressed genes; 88 of those genes were up-regulated in the ALV-J resistant chickens (compared to the susceptible ones). We screened for significantly up-regulated genes (P < 0.01) of interest in the ALV-J resistant chickens, based on their involvement in biological signaling pathways. Functional analyses showed that overexpression of GADD45β inhibited ALV-J replication. GADD45β could enhance defense against ALV-J infection and may be used as a molecular marker to identify ALV-J infections.
authors.affiliation: College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China.
authors.forename: Xinheng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China.
authors.forename: Zhuanqiang
authors.initials: Z
authors.lastname: Yan
authors.affiliation: College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China.
authors.forename: Xinjian
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China.|South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, P. R. China.
authors.forename: Wencheng
authors.initials: W
authors.lastname: Lin
authors.affiliation: College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China.
authors.forename: Zhenkai
authors.initials: Z
authors.lastname: Dai
authors.affiliation: College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Yan
authors.affiliation: College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China.
authors.forename: Piaopiao
authors.initials: P
authors.lastname: Lu
authors.affiliation: College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China.|South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, P. R. China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Chen
authors.affiliation: USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, U.S.A.
authors.forename: Huanmin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China.|South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China.
authors.forename: Jingyun
authors.initials: J
authors.lastname: Ma
authors.affiliation: College of Animal Science, South China Agricultural University & Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642 P. R. China.|Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangdong, Guangzhou 510642, P. R. China.|South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, P. R. China.
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Xie
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry; D001354:Avian Leukosis Virus / Q000502:physiology*; D001715:Bird Diseases / Q000378:metabolism / Q000821:virology; D002645:Chickens; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D017423:Sequence Analysis, RNA; D015398:Signal Transduction; D014779:Virus Replication; D000097642:GADD45 Proteins
chemical_list: D000970:Antineoplastic Agents; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000097642:GADD45 Proteins
keywords: ALV-J; GADD45β; RNA-Seq; differentially expressed genes; viral replication