title: Effects of MC4R, FTO, and NMB gene variants to obesity, physical activity, and eating behavior phenotypes.
abstract: Obesity is a major contributory factor of morbidity and mortality. It has been suggested that biological systems may be involved in the tendency to be and to remain physically inactive also behaviors such as food and beverage preferences and nutrient intake may at least partially genetically determined. Consequently, besides environment, genetic factors may also contribute to the level of physical activity and eating behaviors thus effect obesity. Therefore the aim of this study is to investigate the effect of various gene mutations on obesity, physical activity levels and eating behavior phenotypes. One hundred patients and 100 controls were enrolled to the study. Physical activity levels were measured with an actical acceloremeter device. Eating behaviors were evaluated using Three-Factor Eating questionnaire (TFEQ). Associations between eating behavior scores and physical characteristics were also evaluated. The information about other obesity risk factors were also collected. Mutations were investigated with PCR, direct sequencing and Real-Time PCR. rs1051168, rs8050146 -2778C > T mutations were found statistically significant in patients, rs1121980 was found statistically significant in controls. 21 mutations were found in MC4R and near MC4R of which 18 of them are novel and 8 of them cause amino acid change. In addition, it was found that, some obesity related factors and questions of TFEQ are associated with various investigated gene mutations. Any relation between gene mutations and physical activity levels were not detected. It is thought that, due to the genotype data and eating behaviors, it may be possible to recommend patients for proper eating patterns to prevent obesity. © 2016 IUBMB Life, 68(10):806-816, 2016.
authors.affiliation: Department of Medical Biology, Yeditepe University, Istanbul, Turkey. denizyat@hotmail.com.
authors.forename: Deniz
authors.initials: D
authors.lastname: Kirac
authors.affiliation: Department of Physiology, Marmara University, Istanbul, Turkey.
authors.forename: Ozgur
authors.initials: O
authors.lastname: Kasimay Cakir
authors.affiliation: Department of Medical Genetics, Marmara University, Istanbul, Turkey.
authors.forename: Tuba
authors.initials: T
authors.lastname: Avcilar
authors.affiliation: Department of Endocrinology and Metabolism, Marmara University, Istanbul, Turkey.
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Deyneli
authors.affiliation: Department of Physiology, Marmara University, Istanbul, Turkey.
authors.forename: Hizir
authors.initials: H
authors.lastname: Kurtel
authors.affiliation: Department of Endocrinology and Metabolism, Marmara University, Istanbul, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Yazici
authors.affiliation: Department of Biostatistics, Yeditepe University, Istanbul, Turkey.
authors.forename: Elif Cigdem
authors.initials: EC
authors.lastname: Kaspar
authors.affiliation: Department of Medical Genetics, Marmara University, Istanbul, Turkey.
authors.forename: Nurgul
authors.initials: N
authors.lastname: Celik
authors.affiliation: Department of Medical Genetics, Marmara University, Istanbul, Turkey.
authors.forename: Ahmet Ilter
authors.initials: AI
authors.lastname: Guney
mesh_terms: D000328:Adult; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO / Q000235:genetics*; D001483:Base Sequence; D015992:Body Mass Index; D016022:Case-Control Studies; D015444:Exercise; D005247:Feeding Behavior; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D009154:Mutation; D015287:Neurokinin B / Q000031:analogs & derivatives* / Q000235:genetics; D009765:Obesity / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: C470072:MC4R protein, human; D044105:Receptor, Melanocortin, Type 4; D015287:Neurokinin B; C038393:neuromedin B; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; C526492:FTO protein, human
keywords: FTO; MC4R; NMB; eating behavior; obesity; physical activity