title: Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A.
abstract: The chromokinesin KIF4A has been implicated in shaping mitotic chromosomes, but its functional relationship to condensin complexes remains controversial. Here, we found that, in mitosis, KIF4A associates with condensin I but not with condensin II. Mutational analyses indicated that the enrichment of condensin I to chromosomal axes depends on its association with KIF4A in a way that likely involves its motor activity. Remarkably, this interaction is required for condensin I to confer physiological properties to chromosomes. These observations provide an insight into how condensin I is enriched at chromosomal axes and underscore the significance of axial structure in organizing mitotic chromosomes.
authors.affiliation: Cancer Institute of the Japanese Foundation for Cancer Research (JFCR), Tokyo 135-8550, Japan; Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8112, Japan.
authors.forename: Motoko
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8112, Japan.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Wakai
authors.affiliation: Cancer Institute of the Japanese Foundation for Cancer Research (JFCR), Tokyo 135-8550, Japan;
authors.forename: Toru
authors.initials: T
authors.lastname: Hirota
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D008938:Mitosis; D046912:Multiprotein Complexes / Q000378:metabolism*; D009154:Mutation; D011485:Protein Binding / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; C108312:condensin complexes; D000251:Adenosine Triphosphatases; C484228:KIF4A protein, human; D016547:Kinesins
keywords: SMC; centromere; chromosome condensation; mitosis