title: Mechanisms of electroacupuncture effects on acute cerebral ischemia/reperfusion injury: possible association with upregulation of transforming growth factor beta 1.
abstract: Electroacupuncture at the head acupoints Baihui (GV20) and Shuigou (GV26) improves recovery of neurological function following ischemic cerebrovascular events, but its mechanism remains incompletely understood. We hypothesized that the action of electroacupuncture at these acupoints is associated with elevated serum levels of transforming growth factor beta 1 (TGF-β1). To test this, we established a rat model of cerebral ischemia by middle cerebral artery occlusion. Electroacupuncture was performed at Baihui and Shuigou with a "disperse-dense" wave at an alternating frequency of 2 and 150 Hz, and at a constant intensity of 3 mA. Each electroacupuncture session lasted 30 minutes and was performed every 12 hours for 3 days. Neurological severity scores were lower in injured rats after acupuncture than in those not subjected to treatment. Furthermore, serum level of TGF-β1 was greater after electroacupuncture than after no treatment. Our results indicate that electroacupuncture at Baihui and Shuigou increases the serum level of TGF-β1 in rats with acute cerebral ischemia/reperfusion injury, and exerts neuroprotective effects.
authors.affiliation: Department of Rehabilitation, the First Affiliated Hospital of Xinxiang Medical College, Weihui, Henan Province, China.
authors.forename: Wen-Biao
authors.identifier: 0000-0002-2910-1361
authors.initials: WB
authors.lastname: Wang
authors.affiliation: Department of Rehabilitation, the First Affiliated Hospital of Xinxiang Medical College, Weihui, Henan Province, China.
authors.forename: Lai-Fu
authors.initials: LF
authors.lastname: Yang
authors.affiliation: Department of Rehabilitation, the First Affiliated Hospital of Xinxiang Medical College, Weihui, Henan Province, China.
authors.forename: Qing-Song
authors.initials: QS
authors.lastname: He
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Xinxiang Medical College, Weihui, Henan Province, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Rehabilitation, the First Affiliated Hospital of Xinxiang Medical College, Weihui, Henan Province, China.
authors.forename: Yi-Yong
authors.initials: YY
authors.lastname: Ma
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Xinxiang Medical College, Weihui, Henan Province, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Rehabilitation, the First Affiliated Hospital of Xinxiang Medical College, Weihui, Henan Province, China.
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Cao
keywords: brain injury; cerebral ischemia; inflammatory reaction; nerve injury; nerve regeneration; neural regeneration; neurofunctional rehabilitation; neurological severity score; reperfusion; traditional Chinese medicine; transforming growth factor-beta 1