title: Purification of Lovastatin from Aspergillus terreus (KM017963) and Evaluation of its Anticancer and Antioxidant Properties.
abstract: Cervical cancer is the second most common malignancy in women worldwide and thus one of the leading causes of mortality in women. Lovastatin, a non polar, anticholesterol drug has previously been reported to exert antitumour activity in vitro. In the present study, lovastatin from Aspergillus terreus (KM017963) was purified by adsorption chromatography and evaluated for its anticancer and anti-oxidant properties with a human cervical cancer cell line (HeLa). Growth inhibitory and proapoptotic effects of purified lovastatin on HeLa cells were investigated by determining its influence on cell numbers, mitochondrial membrane potential (MMP), DNA fragmentation and antioxidant properties in terms of hydroxy radical scavenging effects as well as levels of total reduced glutathione. Cell cycle analysis by ow cytometry (propidium iodide staining) confirmed induction of apoptotic cell death and revealed cell cycle arrest in the G0/G1 phase. The results of the study give leads for the anticancer effects of lovastatin and its potential usefulness in the chemotherapy of cervical cancer.
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Bangalore, India E-mail : drsvtj@yahoo.co.in.
authors.forename: Sd
authors.initials: S
authors.lastname: Bhargavi
authors.forename: Vk
authors.initials: V
authors.lastname: Praveen
authors.forename: Salah
authors.initials: S
authors.lastname: Marium
authors.forename: M
authors.initials: M
authors.lastname: Sreepriya
authors.forename: J
authors.initials: J
authors.lastname: Savitha
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000502:physiology*; D001230:Aspergillus / Q000737:chemistry*; D001685:Biological Factors / Q000494:pharmacology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D005260:Female; D016193:G1 Phase / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008148:Lovastatin / Q000737:chemistry* / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D016192:Resting Phase, Cell Cycle / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D001685:Biological Factors; D008148:Lovastatin