title: Direct typing of Canine parvovirus (CPV) from infected dog faeces by rapid mini sequencing technique.
abstract: Canine parvovirus (CPV) is a non-enveloped single stranded DNA virus with an icosahedral capsid. Mini-sequencing based CPV typing was developed earlier to detect and differentiate all the CPV types and FPV in a single reaction. This technique was further evaluated in the present study by performing the mini-sequencing directly from fecal samples which avoided tedious virus isolation steps by cell culture system. Fecal swab samples were collected from 84 dogs with enteritis symptoms, suggestive of parvoviral infection from different locations across India. Seventy six of these samples were positive by PCR; the subsequent mini-sequencing reaction typed 74 of them as type 2a virus, and 2 samples as type 2b. Additionally, 25 of the positive samples were typed by cycle sequencing of PCR products. Direct CPV typing from fecal samples using mini-sequencing showed 100% correlation with CPV typing by cycle sequencing. Moreover, CPV typing was achieved by mini-sequencing even with faintly positive PCR amplicons which was not possible by cycle sequencing. Therefore, the mini-sequencing technique is recommended for regular epidemiological follow up of CPV types, since the technique is rapid, highly sensitive and high capacity method for CPV typing.
authors.affiliation: Translational Research Platform for Veterinary Biologicals, DBT-TANUVAS Partnership Program, Chennai 600051, India.
authors.forename: Pavana Jyothi
authors.initials: PJ
authors.lastname: V
authors.affiliation: Translational Research Platform for Veterinary Biologicals, DBT-TANUVAS Partnership Program, Chennai 600051, India.
authors.forename: Akila
authors.initials: A
authors.lastname: S
authors.affiliation: Translational Research Platform for Veterinary Biologicals, DBT-TANUVAS Partnership Program, Chennai 600051, India.
authors.forename: Malini K
authors.initials: MK
authors.lastname: Selvan
authors.affiliation: Research and Development Laboratory, National Dairy Development Board, c/o Indian Immunologicals Limited, Hyderabad 500032, India; Department of Biotechnology, Acharya Nagarjuna University, Guntur 522510, India.
authors.forename: Hariprasad
authors.initials: H
authors.lastname: Naidu
authors.affiliation: Translational Research Platform for Veterinary Biologicals, DBT-TANUVAS Partnership Program, Chennai 600051, India.
authors.forename: Shwethaa
authors.initials: S
authors.lastname: Raghunathan
authors.affiliation: Translational Research Platform for Veterinary Biologicals, DBT-TANUVAS Partnership Program, Chennai 600051, India.
authors.forename: Sathish
authors.initials: S
authors.lastname: Kota
authors.affiliation: Peripheral Veterinary Hospital, Madhavaram Milk Colony, TANUVAS, Chennai 600051, India.
authors.forename: R C Raja
authors.initials: RC
authors.lastname: Sundaram
authors.affiliation: Research and Development Laboratory, National Dairy Development Board, c/o Indian Immunologicals Limited, Hyderabad 500032, India.
authors.forename: Samir Kumar
authors.initials: SK
authors.lastname: Rana
authors.affiliation: Translational Research Platform for Veterinary Biologicals, DBT-TANUVAS Partnership Program, Chennai 600051, India.
authors.forename: G Dhinakar
authors.initials: GD
authors.lastname: Raj
authors.affiliation: Adviser, National Dairy Development Board, Anand, India.
authors.forename: V A
authors.initials: VA
authors.lastname: Srinivasan
authors.affiliation: Translational Research Platform for Veterinary Biologicals, DBT-TANUVAS Partnership Program, Chennai 600051, India. Electronic address: bhaskaran.mohana@gmail.com.
authors.forename: B
authors.initials: B
authors.lastname: Mohana Subramanian
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004283:Dog Diseases / Q000175:diagnosis / Q000821:virology*; D004285:Dogs; D004751:Enteritis / Q000662:veterinary / Q000821:virology; D005243:Feces / Q000821:virology*; D058889:Molecular Typing / Q000379:methods*; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral
keywords: CPV; Mini-sequencing; Virus typing