title: The role of autophagy in lung ischemia/reperfusion injury after lung transplantation in rats.
abstract: BACKGROUND
  The aim of this study was to explore the role of autophagy in the cold I/R injury following lung transplantation.
  
  
  METHODS
  The rat orthotopic lung transplantation model was established to perform the level of autophagy in the cold I/R injury in this study. The pretreatment of inhibitor (3-Methyladenine [3-MA]) and activator (rapamycin [RAPA]) of autophagy were performed to assess the role of autophagy in the cold I/R injury following lung transplantation in rats.
  
  
  RESULTS
  After lung transplantation, the autophagy, lung cell apoptosis and lung injury were aggravated and peaked at 6 h following the transplantation. The inhibition of autophagy by 3-MA induced downregulated of autophagy, decreased cell apoptosis. Meanwhile, the lung injury, which was indicated by calculating the peak inspiratory pressure (PIP), pulmonary vein blood gas analysis (PO2) and ratio of wet to dry in lung (W/D), was ameliorated after treatment with 3-MA. The activation of autophagy by RAPA causing the upregulated of autophagy and apoptosis of lung cells, and enhanced the lung injury.
  
  
  CONCLUSIONS
  All the results suggested that the autophagy was involved in the cold I/R injury in lung transplantation model, and played a potential role on the regulation of I/R injury after lung transplantation.
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Nanchang University Jiangxi, PR China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Nanchang University Jiangxi, PR China.
authors.forename: Bentong
authors.initials: B
authors.lastname: Yu
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Nanchang University Jiangxi, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Nanchang University Jiangxi, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Dai
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Nanchang University Jiangxi, PR China.
authors.forename: Jichun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Nanchang UniversityJiangxi, PR China; Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyWuhan, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Tang
keywords: Autophagy; lung ischemia/reperfusion injury; lung transplantation