title: Extracellular matrix from human umbilical cord-derived mesenchymal stem cells as a scaffold for peripheral nerve regeneration.
abstract: The extracellular matrix, which includes collagens, laminin, or fibronectin, plays an important role in peripheral nerve regeneration. Recently, a Schwann cell-derived extracellular matrix with classical biomaterial was used to mimic the neural niche. However, extensive clinical use of Schwann cells remains limited because of the limited origin, loss of an autologous nerve, and extended in vitro culture times. In the present study, human umbilical cord-derived mesenchymal stem cells (hUCMSCs), which are easily accessible and more proliferative than Schwann cells, were used to prepare an extracellular matrix. We identified the morphology and function of hUCMSCs and investigated their effect on peripheral nerve regeneration. Compared with a non-coated dish tissue culture, the hUCMSC-derived extracellular matrix enhanced Schwann cell proliferation, upregulated gene and protein expression levels of brain-derived neurotrophic factor, glial cell-derived neurotrophic factor, and vascular endothelial growth factor in Schwann cells, and enhanced neurite outgrowth from dorsal root ganglion neurons. These findings suggest that the hUCMSC-derived extracellular matrix promotes peripheral nerve repair and can be used as a basis for the rational design of engineered neural niches.
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China; The Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong, Jiangsu Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiao
authors.affiliation: Department of Orthopedics, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Rao
authors.affiliation: Department of Orthopedics, Cangzhou Central Hospital, Cangzhou, Hebei Province, China.
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Guo
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Sun
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
authors.forename: Yi-Guo
authors.initials: YG
authors.lastname: Wang
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
authors.forename: Shu-Yun
authors.initials: SY
authors.lastname: Liu
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
authors.forename: Ai-Yuan
authors.initials: AY
authors.lastname: Wang
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
authors.forename: Quan-Yi
authors.initials: QY
authors.lastname: Guo
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
authors.forename: Hao-Ye
authors.initials: HY
authors.lastname: Meng
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; The Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong, Jiangsu Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; The Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong, Jiangsu Province, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Peng
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; The Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong, Jiangsu Province, China.
authors.forename: Yu
authors.identifier: 0000-0001-7811-5834
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China; Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Beijing, China.
authors.forename: Shi-Bi
authors.identifier: 0000-0001-5333-4061
authors.initials: SB
authors.lastname: Lu
keywords: Schwann cell; collagen; dorsal root ganglion neuron; extracellular matrix; fibronectin; laminin; mesenchymal stem cells; nerve regeneration; neural regeneration; neurotropic factors; niche; peripheral nerve regeneration