title: Remineralizing potential of a 60-s in vitro application of Tooth Mousse Plus.
abstract: BACKGROUND
  No published studies exist on the remineralizing potential of Tooth Mousse Plus®(TMP) when applied for less than 3 min.
  
  
  OBJECTIVE
  To evaluate (i) the remineralizing potential of TMP on artificial carious lesions, when applied thrice daily for 60 s, and (ii) the benefit of using a fluoridated dentifrice prior to TMP application.
  
  
  METHODS
  Carious lesions, 120-200 μm deep, were produced by placing molars in demineralizing solution for 96 h, and sections 100-150 μm thick were then randomly assigned to four groups. Specimens were treated thrice daily with a non-fluoridated (Group A), or 1000 ppm F dentifrice (Group B), or TMP (Group C), or a 1000 ppm F dentifrice followed by TMP application (Group D), and then subjected to a 10-day pH cycling model. Lesion evaluation involved polarizing light microscopy and microradiography.
  
  
  RESULTS
  Post-treatment maximum mineral content at the surface zone (Vmax) was significantly increased and lesion depth (LD) significantly decreased in Group C, while only the Vmaxincreased in Group D. Increase in LD was observed in Group B; however, no significant differences were noted in percentage LD changes between groups B, C, and D (P > 0.05).
  
  
  CONCLUSIONS
  TMP applied for 60 s significantly remineralized the artificial carious lesions. No additional benefit was evident when TMP was preceded by treatment with 1000 ppm F dentifrice.
authors.affiliation: Paediatric Dentistry, School of Dentistry, The University of Western Australia, Nedlands, Australia.
authors.forename: Vaishnavi L
authors.initials: VL
authors.lastname: Gopalakrishnan
authors.affiliation: Paediatric Dentistry, School of Dentistry, The University of Western Australia, Nedlands, Australia.
authors.forename: Robert P
authors.initials: RP
authors.lastname: Anthonappa
authors.affiliation: Paediatric Dentistry, School of Dentistry, The University of Western Australia, Nedlands, Australia.
authors.forename: Nigel M
authors.initials: NM
authors.lastname: King
authors.affiliation: Paediatric Dentistry, School of Dentistry and Oral Health, Griffith University, Gold Coast, Australia.
authors.forename: Anut
authors.initials: A
authors.lastname: Itthagarun
mesh_terms: D002327:Cariostatic Agents / Q000009:adverse effects; D002364:Caseins / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D003731:Dental Caries / Q000188:drug therapy* / Q000473:pathology; D003743:Dental Enamel / Q000737:chemistry / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology; D003764:Dental Materials; D003802:Dentifrices / Q000008:administration & dosage / Q000494:pharmacology; D005457:Fluoridation; D005459:Fluorides / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008422:Materials Testing; D008852:Microradiography; D008859:Microscopy, Polarization; D008964:Molar, Third / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology; D013997:Time Factors; D014089:Tooth Remineralization; D014100:Toothpastes / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D002327:Cariostatic Agents; D002364:Caseins; D003764:Dental Materials; D003802:Dentifrices; D014100:Toothpastes; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex; D005459:Fluorides