title: Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces.
abstract: Polyaniline shows great potential and promises wide application in the biomedical field thanks to its intrinsic conductivity and material properties, which closely resemble natural tissues. Surface properties are crucial, as these predetermine any interaction with biological fluids, proteins and cells. An advantage of polyaniline is the simple modification of its surface, e.g., by using various dopant acids. An investigation was made into the adhesion, proliferation and migration of mouse embryonic fibroblasts on pristine polyaniline films and films doped with sulfamic and phosphotungstic acids. In addition, polyaniline films supplemented with poly (2-acrylamido-2-methyl-1-propanesulfonic) acid at various ratios were tested. Results showed that the NIH/3T3 cell line was able to adhere, proliferate and migrate on the pristine polyaniline films as well as those films doped with sulfamic and phosphotungstic acids; thus, utilization of said forms in biomedicine appears promising. Nevertheless, incorporating poly (2-acrylamido-2-methyl-1-propanesulfonic) acid altered the surface properties of the polyaniline films and significantly affected cell behavior. In order to reveal the crucial factor influencing the surface/cell interaction, cell behavior is discussed in the context of the surface energy of individual samples. It was clearly demonstrated that the lesser the difference between the surface energy of the sample and cell, the more cyto-compatible the surface is.
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín, třída Tomáše Bati 5678, 760 01 Zlín, Czech Republic. rejmontova@cps.utb.cz.
authors.forename: Petra
authors.initials: P
authors.lastname: Rejmontová
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín, třída Tomáše Bati 5678, 760 01 Zlín, Czech Republic. kucekova@cps.utb.cz.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Capáková
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín, třída Tomáše Bati 5678, 760 01 Zlín, Czech Republic. mikusova@ft.utb.cz.
authors.forename: Nikola
authors.initials: N
authors.lastname: Mikušová
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín, třída Tomáše Bati 5678, 760 01 Zlín, Czech Republic. marakova@ft.utb.cz.
authors.forename: Nela
authors.initials: N
authors.lastname: Maráková
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín, třída Tomáše Bati 5678, 760 01 Zlín, Czech Republic. vkasparkova@ft.utb.cz.
authors.forename: Věra
authors.initials: V
authors.lastname: Kašpárková
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín, třída Tomáše Bati 5678, 760 01 Zlín, Czech Republic. lehocky@ft.utb.cz.
authors.forename: Marián
authors.initials: M
authors.lastname: Lehocký
authors.affiliation: Centre of Polymer Systems, Tomas Bata University in Zlín, třída Tomáše Bati 5678, 760 01 Zlín, Czech Republic. humpolicek@ft.utb.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Humpolíček
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D051379:Mice; D041681:NIH 3T3 Cells; D013499:Surface Properties
chemical_list: D000814:Aniline Compounds; D001672:Biocompatible Materials; C416807:polyaniline
keywords: cyto-compatibility; fibroblast; phosphotungstic acid; poly (2-acrylamido-2-methyl-1-propanesulfonic) acid; polyaniline; sulfamic acid