title: Monitoring of human herpesviruses-6 and -7 DNA in saliva samples during the acute and convalescent phases of exanthem subitum.
abstract: The amounts of the DNAs of human herpesviruses-6 (HHV-6) and -7 (HHV-7) in saliva samples were monitored during the acute and convalescent phases of exanthem subitum (ES) to elucidate the kinetics of virus shedding after ES. A total of 247 saliva samples were collected from 17 children (5 males and 12 females: 8-31 months old at onset). The monitoring period ranged from 152 to 721 days after onset, and in 15 children it was longer than 1 year. Among the 17 cases, 16 were attributed to HHV-6B, while a single case was attributed to HHV-7. Detection rates and average amounts of HHV-6 DNA in saliva samples after ES attributed to HHV-6B were low in the acute phase, increased to the maximum in the convalescent phase at 3-7 months, and then decreased. In addition, to investigate the source of infection, saliva samples from the older siblings (age 3-9 years) and parents of ES patients and children with a history of ES were also examined. The detection rate of HHV-6 DNA in saliva samples from 3- to 9-year-old children was significantly higher than the rate in adult saliva samples. Taken together, these findings suggest that the saliva of children in the convalescent phase of ES might be a more likely source of HHV-6 infection than that of adults. J. Med. Virol. 89:696-702, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Departments of Virology and Pediatrics, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Miyazaki
authors.affiliation: Departments of Virology and Pediatrics, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Namba
authors.affiliation: Aquair Medical Station, Yokkaichi, Mie, Japan.
authors.forename: Sadayoshi
authors.initials: S
authors.lastname: Torigoe
authors.affiliation: Suzuka Pediatrics, Suzuka, Mie, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Departments of Virology and Pediatrics, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan.|Department of Pediatrics, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Yamashita
authors.affiliation: Departments of Virology and Pediatrics, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: Hirohito
authors.initials: H
authors.lastname: Ogawa
authors.affiliation: Department of Pediatrics, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan.|Okayama Rosai Hospital, Okayama, Japan.
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Morishima
authors.affiliation: Departments of Virology and Pediatrics, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Yamada
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D005077:Exanthema Subitum / Q000821:virology*; D005260:Female; D015654:Herpesvirus 6, Human / Q000235:genetics*; D016199:Herpesvirus 7, Human / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D012463:Saliva / Q000821:virology*; D013997:Time Factors; D017201:Virus Shedding
chemical_list: D004279:DNA, Viral
keywords: beta-herpesviruses; real-time PCR; roseola infantum; source of infection; virus isolation; virus shedding