title: A biodistribution study of two differently shaped plant virus nanoparticles reveals new peculiar traits.
abstract: Self-assembling plant virus nanoparticles (pVNPs) have started to be explored as nanometre-sized objects for biomedical applications, such as vaccine or drug delivery and imaging. Plant VNPs may be ideal tools in terms of biocompatibility and biodegradability endowed with a wide diversity of symmetries and dimensions, easy chemical/biological engineering, and rapid production in plants. Recently, we defined that icosahedral Tomato bushy stunt virus (TBSV) and filamentous Potato virus X (PVX) are neither toxic nor teratogenic. We report here the results of an interdisciplinary study aimed to define for the first time the biodistribution of unlabelled, unpegylated, underivatized TBSV and PVX by proved detecting antibodies. These data add new insights on the in vivo behaviour of these nano-objects and demonstrate that the pVNPs under scrutiny are each intrinsically endowed with peculiar properties foreshadowing different applications in molecular medicine.
authors.affiliation: Laboratory of Biotechnology, Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile (ENEA), Rome, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Lico
authors.affiliation: Department of Radiation Physics, Guglielmo Marconi University, Rome, Italy; Department of Sciences, University of Roma Tre, Rome, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Giardullo
authors.affiliation: Laboratory of Biomedical technologies, Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile (ENEA), Rome, Italy.
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Mancuso
authors.affiliation: Laboratory of Biotechnology, Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile (ENEA), Rome, Italy.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Benvenuto
authors.affiliation: Department of Agricultural and Forestry Sciences (DAFNE), University of Tuscia, Viterbo, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Santi
authors.affiliation: Laboratory of Biotechnology, Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile (ENEA), Rome, Italy. Electronic address: selene.baschieri@enea.it.
authors.forename: Selene
authors.initials: S
authors.lastname: Baschieri
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D001921:Brain / Q000378:metabolism / Q000821:virology; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology / Q000493:pharmacokinetics*; D016679:Genome, Viral / Q000235:genetics; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism / Q000821:virology; D008099:Liver / Q000378:metabolism / Q000821:virology; D008168:Lung / Q000378:metabolism / Q000821:virology; D051379:Mice; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000378:metabolism* / Q000648:ultrastructure; D017863:Potexvirus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D013154:Spleen / Q000378:metabolism / Q000821:virology; D013997:Time Factors; D014018:Tissue Distribution; D014026:Nicotiana / Q000821:virology; D017864:Tombusvirus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D014771:Virion / Q000235:genetics / Q000502:physiology; D014777:Virus Diseases / Q000378:metabolism* / Q000821:virology
chemical_list: D000914:Antibodies, Viral
keywords: Biodistribution; ELISA; Immunohistochemistry; Plant virus nanoparticles; Potato virus X; Tomato bushy stunt virus