title: The involvement of ROS producing aldehyde oxidase in plant response to Tombusvirus infection.
abstract: The influence of Tomato bushy stunt virus (TBSV) infection on the activity and isoformic composition of aldehyde oxidase and catalase in Nicotiana benthamiana plants was investigated. It was shown that the infection of plants with TBSV results in enhancement of leaf aldehyde oxidase (AO) isoforms AO2 and AO3. Significantly enhanced levels of superoxide radical producing activity of AO isoforms were also detected. This is the first demonstration of involvement of plant AO in defense mechanisms against viral infection. In addition, the infection caused an increased accumulation of hydrogen peroxide, compared to mock-inoculated plants. The virus infection resulted in increased activity of catalase (CAT) and superoxide dismutase (SOD) in roots and leaves of N. benthamiana. Moreover, activation of two additional CAT isoforms was observed in the leaves of plants after virus inoculation. Our findings indicate that the virus infection significantly affects enzymes responsible for the balance of ROS accumulation in plant tissue in response to pathogen attack.
authors.affiliation: Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian University, Astana, Kazakhstan.
authors.forename: Timur M
authors.initials: TM
authors.lastname: Yergaliyev
authors.affiliation: Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian University, Astana, Kazakhstan.
authors.forename: Zhadyrassyn
authors.initials: Z
authors.lastname: Nurbekova
authors.affiliation: Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian University, Astana, Kazakhstan.
authors.forename: Gulzhamal
authors.initials: G
authors.lastname: Mukiyanova
authors.affiliation: Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian University, Astana, Kazakhstan.
authors.forename: Alua
authors.initials: A
authors.lastname: Akbassova
authors.affiliation: Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian University, Astana, Kazakhstan.
authors.forename: Maxim
authors.initials: M
authors.lastname: Sutula
authors.affiliation: Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian University, Astana, Kazakhstan; Department of Biology and Ecology, S.Toraighyrov Pavlodar State University, Pavlodar, Kazakhstan.
authors.forename: Sayan
authors.initials: S
authors.lastname: Zhangazin
authors.affiliation: Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian University, Astana, Kazakhstan.
authors.forename: Assyl
authors.initials: A
authors.lastname: Bari
authors.affiliation: Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian University, Astana, Kazakhstan.
authors.forename: Zhanerke
authors.initials: Z
authors.lastname: Tleukulova
authors.affiliation: The Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan.
authors.forename: Malika
authors.initials: M
authors.lastname: Shamekova
authors.affiliation: Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian University, Astana, Kazakhstan.
authors.forename: Zhaksylyk K
authors.initials: ZK
authors.lastname: Masalimov
authors.affiliation: Department of Biotechnology and Microbiology, L.N. Gumilyov Eurasian University, Astana, Kazakhstan. Electronic address: omarov_rt@enu.kz.
authors.forename: Rustem T
authors.initials: RT
authors.lastname: Omarov
mesh_terms: D042931:Aldehyde Oxidase / Q000378:metabolism*; D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006861:Hydrogen Peroxide / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000201:enzymology / Q000378:metabolism / Q000821:virology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D017864:Tombusvirus / Q000254:growth & development* / Q000502:physiology
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D013481:Superoxides; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D042931:Aldehyde Oxidase
keywords: Aldehyde oxidase; Catalase; Hydrogen peroxide; N. benthamiana; Reactive oxygen species; Superoxide dismutase; Tomato bushy stunt virus