title: Accelerated bony defect healing based on chitosan thermosensitive hydrogel scaffolds embedded with chitosan nanoparticles for the delivery of BMP2 plasmid DNA.
abstract: Regeneration of alveolar bone is among the main purposes of periodontal treatment. However, it is a challenge for both dentists and patients. An injectable chitosan-based thermosensitive hydrogel scaffold (CS/CSn-GP) was successfully designed and prepared with the incorporation of bone morphogenetic protein-2 plasmid DNA (pDNA-BMP2)-loaded chitosan nanoparticles (CS/CSn(pDNA-BMP2)-GP) in this study. The aim of this study was to evaluate the regenerative effect of this scaffold on the endogenous repair of alveolar bone. Thermosensitive properties, SEM scans and in vivo inflammatory reactions and degradation were evaluated. The CS/CSn-GP solution was initially injected into the muscle pouches of rats, where it changed into a gel, resulting in non-specific inflammation. It effectively enhanced new bone formation in rat calvarial defects and enhanced bony defect healing in beagle dogs. All results indicated that the CS/CSn(pDNA-BMP2)-GP complex system is a potential candidate as a targeted, multi-effect scaffold for the endogenous repair of alveolar bone. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 265-273, 2017.
authors.affiliation: Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266001, China.|School of Stomatology, Qingdao University, Qingdao, Shandong, 266001, China.|Department of Stomatology, Beijing Tongzhou Xinhua Hospital, Tongzhou, Beijing, 101100, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266001, China.|School of Stomatology, Qingdao University, Qingdao, Shandong, 266001, China.
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Ji
authors.affiliation: Orthopedic Center, Qilu Hospital of Shandong University, Qingdao, Shandong, 266035, China.
authors.forename: Ximin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266001, China.|School of Stomatology, Qingdao University, Qingdao, Shandong, 266001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266001, China.|School of Stomatology, Qingdao University, Qingdao, Shandong, 266001, China.
authors.forename: Quanchen
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266001, China.|School of Stomatology, Qingdao University, Qingdao, Shandong, 266001, China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Deng
authors.affiliation: Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266001, China.|School of Stomatology, Qingdao University, Qingdao, Shandong, 266001, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Hu
authors.affiliation: Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266001, China.|School of Stomatology, Qingdao University, Qingdao, Shandong, 266001, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Yang
mesh_terms: D000539:Alveolar Process / Q000293:injuries / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis / Q000235:genetics; D001861:Bone Regeneration / Q000187:drug effects* / Q000235:genetics; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D004285:Dogs; D018014:Gene Transfer Techniques; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use; D010510:Periodontal Diseases / Q000628:therapy*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D020100:Hydrogels; D048271:Chitosan
keywords: BMP2; chitosan nanoparticles; endogenous repair; thermosensitive hydrogel; tissue engineering scaffold