title: Decreased expression of IDH1-R132H correlates with poor survival in gastrointestinal cancer.
abstract: Isocitrate dehydrogenase (IDH1) is an NADP-dependent enzyme that catalyzes the decarboxylation of isocitrate to alpha-ketoglutarate. The IDH1-R132H mutation predicts a better clinical outcome for glioma patients, and the expression of IDH1-R132H correlates with a favorable outcome in patients with brain tumors. Here, we investigated IDH1-R132H expression in both gastric (n=526) and colorectal (n=399) tissues by performing immunohistochemistry analyses on tissue microarrays. We also tested whether IDH1-R132H expression correlated with various clinical parameters. In both gastric and colorectal cancer, expression of IDH1-R132H was associated with tumor stage. Patients with low IDH1-R132H expression had a poor overall survival. Our data indicate that IDH1-R132H expression could be used as a predictive marker of prognosis for patients with gastrointestinal cancer.
authors.affiliation: Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
authors.forename: Jieying
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Laboratory Medicine & Department of Clinical Tissue Bank, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NC, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D019943:Amino Acid Substitution; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D003062:Codon; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007521:Isocitrate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009154:Mutation; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D003062:Codon; D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human
keywords: IDH1-R132H; gastrointestinal cancer; immunohistochemistry; prognosis; tissue microarray