title: Development and application of a double- antibody sandwich ELISA kit for the detection of serum MUC1 in lung cancer patients.
abstract: BACKGROUND
  Mucin 1 (MUC1), as an oncogene, plays an important role in the diagnosis of lung cancer.
  
  
  OBJECTIVE
  To establish a double-antibody sandwich enzyme-linked immune sorbent assay (ELISA) kit for the detection of serum MUC1 in lung cancer patients.
  
  
  METHODS
  Commercial mouse anti-human MUC1 monoclonal antibody and rabbit anti-human MUC1 polyclonal antibody were used to construct a double-antibody sandwich ELISA kit. The serum MUC1 levels in peripheral blood of lung disease patients and healthy individuals were detected by the double-antibody sandwich ELISA kit and CA15-3 kit, respectively.
  
  
  RESULTS
  A double-antibody sandwich ELISA kit was successfully constructed, and the sensitivity was up to 0.5 μ g/l. The cut-off value for the serum MUC1 levels in the peripheral blood was 1.98 μ g/l, the sensitivity was 62.5%, the specificity was 100% and the Youden index was 0.6250 when detected by the double-antibody sandwich ELISA kit, while the sensitivity was 18.75%, the specificity was 100% and the Youden index was 0.1875 when detected by CA15-3 kit.
  
  
  CONCLUSIONS
  The double-antibody sandwich ELISA kit is superior to the CA15-3 kit in the detection of serum MUC1 in lung cancer patients, suggesting an attractive applying of the double-antibody sandwich ELISA kit in the early diagnosis of lung cancer.
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, China.|Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Biochemistry, Basic Medical School, Changchun Medical College, Changchun, Jilin, China.|Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Ni
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhai
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xie
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Hematology and Oncology, The First Bethune Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Sujun
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Guixiang
authors.initials: G
authors.lastname: Tai
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood*; D008297:Male; D051379:Mice; D008875:Middle Aged; D018396:Mucin-1 / Q000097:blood*; D011379:Prognosis; D011817:Rabbits; D015996:Survival Rate
chemical_list: C060192:MUC1 protein, human; D018396:Mucin-1
keywords: CA15-3; ELISA; MUC1; anti-MUC1 polyclonal antibody; lung cancer