title: Monitoring the synthesis of biomolecules using mass spectrometry.
abstract: The controlled and selective synthesis/clearance of biomolecules is critical for most cellular processes. In most high-throughput 'omics' studies, we measure the static quantities of only one class of biomolecules (e.g. DNA, mRNA, proteins or metabolites). It is, however, important to recognize that biological systems are highly dynamic in which biomolecules are continuously renewed and different classes of biomolecules interact and affect each other's production/clearance. Therefore, it is necessary to measure the turnover of diverse classes of biomolecules to understand the dynamic nature of biological systems. Herein, we explain why the kinetic analysis of a diverse range of biomolecules is important and how such an analysis can be done. We argue that heavy water ((2)H2O) could be a universal tracer for monitoring the synthesis of biomolecules on a global scale.This article is part of the themed issue 'Quantitative mass spectrometry'.
authors.affiliation: Center for Proteomics and Bioinformatics, Department of Nutrition, Case Western Reserve University, Cleveland, OH 44106, USA masaru.miyagi@case.edu.
authors.forename: Masaru
authors.identifier: http://orcid.org/0000-0003-4325-1216
authors.initials: M
authors.lastname: Miyagi
authors.affiliation: Mass Spectrometry Laboratory, Department of Pharmaceutical Sciences, Northeast Ohio Medical University, Rootstown, OH 44272, USA.
authors.forename: Takhar
authors.identifier: http://orcid.org/0000-0003-2173-7231
authors.initials: T
authors.lastname: Kasumov
mesh_terms: D017666:Deuterium Oxide / Q000737:chemistry; D007700:Kinetics; D046911:Macromolecular Substances / Q000737:chemistry* / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D046911:Macromolecular Substances; D017666:Deuterium Oxide
keywords: biosynthesis; deuterium water; flux analysis; mass spectrometry; metabolomics; proteomics