title: Reproductive and neurobehavioral effects of maternal exposure to piperonyl butoxide (PBO) in F1-generation mice.
abstract: Female mice were exposed maternally to piperonyl butoxide (PBO) through diet to provide levels of 0 (control), 0.015, 0.03, and 0.06% during gestation and lactation periods, and selected reproductive and neurobehavioral parameters were measured in F1generation. There was no adverse effect of PBO on litter size, litter weight, or sex ratio at birth. The average body weights of offspring showed no significant effects of PBO treatment through the lactation period in both sexes except for the low-dose group of females on PND 21. With respect to behavioral developmental parameters, swimming direction of female offspring on PND 7 was significantly accelerated in the low-dose group (p = 0.022). Exploratory behavior examination in male offspring indicated that total distance and movement time shortened significantly in dose-related manners (p = 0.0138 and 0.00231, respectively), average time of rearing lengthened significantly in a dose-related manner (p = 0.00814), and the frequencies of mice with urination was increased significantly in a dose-related manner (p < 0.05). For spontaneous behavior examination, the average time of movement in males and average time of rearing in females showed slightly dose-related effects in the F1generation. The dose levels of PBO in the present study produced some adverse effects in neurobehavioral parameters in mice.
authors.affiliation: Division of Toxicology, Department of Pharmaceutical and Environmental Sciences, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan. t-tanaka@poohlover.net.
authors.forename: Toyohito
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Division of Toxicology, Department of Pharmaceutical and Environmental Sciences, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Inomata
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005106:Exploratory Behavior / Q000187:drug effects; D005260:Female; D008098:Litter Size / Q000187:drug effects; D008124:Locomotion / Q000187:drug effects; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D010882:Piperonyl Butoxide / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000503:physiopathology*; D012098:Reproduction / Q000187:drug effects*; D018675:Toxicity Tests
chemical_list: D010882:Piperonyl Butoxide
keywords: behavioral development; exploratory behavior; maternal exposure; maze learning; mice; movement activity; piperonyl butoxide (PBO); reproductive toxicity; spontaneous behavior