title: Effect of co-transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of hepatocellular carcinoma Huh7.
abstract: OBJECTIVE
  To investigate the effects of co-transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of hepatocellular carcinoma Huh7.
  
  
  METHODS
  Hepatocellular carcinoma Huh7 was cultured in vitro and lipidosome was used to transfect miR-520c-3p and miR-132, respectively or together. The effects of transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of Huh7 were detected by CCK8 and Annexin V staining and flow cytometry, and the expression level of the targeted gene of over-expressed miR-520c-3p and miR-132 was determined by Western blot and realtime PCR.
  
  
  RESULTS
  Compared with the control group, the proliferation ability of Huh7 of the single transfected and co-transfected miR-520c-3p and miR-132 decreased significantly, and the apoptosis ratio increased distinctly (P < 0.05). Besides, the effect of the co-transfection group was better than that of the single transfection group. The protein levels of GPC3 (Glypican-3) and YAP (Yes-associated protein), the target genes transfected only by miR-520c-3p and miR-132, respectively, reduced obviously (P < 0.05), which was similar with the co-infected cells, but cells transfected by miR-132 only showed a decrease of YAP.
  
  
  CONCLUSIONS
  The co-transfection of miR-520c-3p and miR-132 can target-regulate the expression of GPC3 and YAP, enhance the exhibition effect on proliferation of hepatocellular carcinoma Huh7 and induce cell apoptosis synergistically.
authors.affiliation: Tumor Laboratory, The Fifth Hospital of Wuhan, Wuhan, 430050, Hubei Province, China.
authors.forename: Chang-Jiang
authors.initials: CJ
authors.lastname: Lei
authors.affiliation: Wuhan Hematology Institute, Wuhan, 430050, Hubei Province, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yao
authors.affiliation: Department of Anesthesiology, The Fifth Hospital of Wuhan, Wuhan, 430050, Hubei Province, China.
authors.forename: De-Ke
authors.initials: DK
authors.lastname: Li
authors.affiliation: Oncology Department, The Fifth Hospital of Wuhan, Wuhan, 430050, Hubei Province, China. Electronic address: jgjjgg7698@126.com.
authors.forename: Zhi-Xiong
authors.initials: ZX
authors.lastname: Long
authors.affiliation: Tumor Laboratory, The Fifth Hospital of Wuhan, Wuhan, 430050, Hubei Province, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Oncology Department, The Fifth Hospital of Wuhan, Wuhan, 430050, Hubei Province, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Tao
authors.affiliation: Oncology Department, The Fifth Hospital of Wuhan, Wuhan, 430050, Hubei Province, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Liou
authors.affiliation: Oncology Department, The Fifth Hospital of Wuhan, Wuhan, 430050, Hubei Province, China.
authors.forename: Jiang-Zhou
authors.initials: JZ
authors.lastname: Zhang
authors.affiliation: Tumor Laboratory, The Fifth Hospital of Wuhan, Wuhan, 430050, Hubei Province, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
keywords: Apoptosis; Liver cancer; MiR-132; Proliferation; miR-520c-3p