title: Establishment and characterization of an expanding-type gastric cancer cell line by Ming's classification.
abstract: According to Ming's classification, gastric cancer (GC) can be divided into two types: expanding and infiltrative. The two types are readily recognizable by histology: expanding carcinomas grow en masse and by expansion, resulting in the formation of discrete tumour nodules, whereas in infiltrative carcinoma, tumour cells invade individually. Both types show varying degrees of cell maturation. The two types of carcinomas have vastly different pathological and clinical features. However, little is known concerning the mechanisms underlying these differences since no GC cell line models are available. For comprehensive and insightful analyses of mechanisms and treatment methods, new cell lines derived from expanding- and infiltrative-type gastric tumours are urgently needed. In the present study, we established an expanding-type GC cell line from a 72-year-old male patient. Different in vitro and in vivo methods were used to characterize the phenotypes of this cell line. This GC cell line was named XGC-2 and had an ~60 h doubling time. The cell line displayed strong colony formation and tumourigenicity in nude mice and had complicated chromosomal abnormalities. XGC-2 cells showed some markers of epithelial-to-mesenchymal transition (EMT), with decreased E-cadherin expression levels and increased vimentin expression levels. The XGC-2 cell line may be useful for future studies of GC development, progression, metastasis and therapy.
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian 361005, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian 361005, P.R. China.
authors.forename: Ji-Gui
authors.initials: JG
authors.lastname: Peng
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian 361005, P.R. China.
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Zhuang
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian 361005, P.R. China.
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: Institute of Gastrointestinal Oncology, Medical College of Xiamen University, Xiamen, Fujian 361005, P.R. China.
authors.forename: Qi-Cong
authors.initials: QC
authors.lastname: Luo
authors.affiliation: Institute of Gastrointestinal Oncology, Medical College of Xiamen University, Xiamen, Fujian 361005, P.R. China.
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Huang
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian 361005, P.R. China.
authors.forename: Chun-Dong
authors.initials: CD
authors.lastname: Lin
authors.affiliation: Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian 361005, P.R. China.
authors.forename: Jian-Chun
authors.initials: JC
authors.lastname: Cai
mesh_terms: D000368:Aged; D000818:Animals; D015820:Cadherins / Q000096:biosynthesis; D002277:Carcinoma / Q000473:pathology*; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D013274:Stomach Neoplasms / Q000473:pathology*; D014746:Vimentin / Q000096:biosynthesis; D023041:Xenograft Model Antitumor Assays
chemical_list: D015820:Cadherins; D014746:Vimentin