title: Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts.
abstract: Near-infrared fluorescence (NIRF) imaging technology is a highly sensitive imaging modality and has been widely used in noninvasively studying the status of receptor expression in small animal models, with an appropriate NIRF probe targeting a specific receptor. In this report, Cy5.5-conjugated anti-CAIX monoclonal antibody (Mab-Cy5.5) was evaluated in athymic mice bearing HT-29 tumor xenografts in order to investigate the effect of conjugate on tumor targeting efficacy. In vitro binding studies showed that Mab-Cy5.5 could specifically bind to the cells which expressed CAIX. Results from in vivo imaging showed that HT-29 tumor xenografts can be clearly visualized at 48 h after injection of Mab-Cy5.5, and in the blocking experiment, free anti-CAIX antibody effectively blocked the concentration of Mab-Cy5.5 in the tumors. Western blotting and immunohistochemistry analysis of HT-29 tumor xenografts verified the expression of CAIX in HT-29 tumors. Mab-Cy5.5 could specifically bind to the tumors which expressed CAIX. These results suggested that Mab-Cy5.5 was suitable for CAIX expression imaging in the preclinical research.
authors.affiliation: Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital, Hohhot 010050, China.
authors.forename: Jianbo
authors.identifier: 0000-0003-0031-1645
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital, Hohhot 010050, China.
authors.forename: Baoliang
authors.identifier: 0000-0002-0267-2359
authors.initials: B
authors.lastname: Bao
authors.affiliation: Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital, Hohhot 010050, China.
authors.forename: Lei
authors.identifier: 0000-0003-0890-3581
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital, Hohhot 010050, China.
authors.forename: Xuemei
authors.identifier: 0000-0002-8464-641X
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002232:Carbocyanines; D000071231:Carbonic Anhydrase IX / Q000378:metabolism*; D003287:Contrast Media; D019073:HT29 Cells; D006801:Humans; D007259:Infrared Rays; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging / Q000201:enzymology* / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D003287:Contrast Media; D000071231:Carbonic Anhydrase IX; C492796:Car9 protein, mouse