title: Inhibitory effect of magnetic Fe3O4nanoparticles coloaded with homoharringtonine on human leukemia cells in vivo and in vitro.
abstract: Homoharringtonine (HHT), a natural cephalotaxine alkaloid, has been used in the People's Republic of China for treatment of leukemia for >3 decades. Here, we employed magnetic Fe3O4nanoparticles (MNP-Fe3O4) to improve the therapeutic effect of HHT and investigated its biological effects. Within a certain range of concentrations, the HHT-MNP-Fe3O4showed a more enhanced inhibitory effect on the selected myeloid leukemia cell lines than HHT alone. Compared with HHT, HHT-MNP-Fe3O4could induce more extensive apoptosis in leukemia cells, which also showed more pronounced cell arrests at G0/G1 phase. HHT-MNP-Fe3O4enhanced antitumor activity by downregulating myeloid cell leukemia-1, which could inhibit the activation of caspase-3 and poly-ADP-ribose polymerase. In vivo experiments using tumor-bearing animal models showed that the mean tumor volume with HHT-MNP-Fe3O4was significantly smaller than that with HHT alone (193±26 mm3versus 457±100 mm3,P<0.05), while the mean weight was 0.67±0.03 g versus 1.42±0.56 g (P<0.05). Immunohistochemical study showed fewer myeloid cell leukemia-1-stained cells in mice treated with HHT-MNP-Fe3O4than with the controls. These findings provide a more efficient delivery system for HHT in the treatment of hematological malignancy.
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Soochow University, Suzhou; Collaborative Innovation Center of Hematology, Soochow University, Suzhou.
authors.forename: Meiyu
authors.initials: M
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing; Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiong
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Soochow University, Suzhou; Collaborative Innovation Center of Hematology, Soochow University, Suzhou.
authors.forename: Liang
authors.initials: L
authors.lastname: Ma
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Soochow University, Suzhou; Collaborative Innovation Center of Hematology, Soochow University, Suzhou.
authors.forename: Hong
authors.initials: H
authors.lastname: Yao
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Soochow University, Suzhou; Collaborative Innovation Center of Hematology, Soochow University, Suzhou.
authors.forename: Qinrong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Soochow University, Suzhou; Collaborative Innovation Center of Hematology, Soochow University, Suzhou.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wen
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Soochow University, Suzhou; Collaborative Innovation Center of Hematology, Soochow University, Suzhou.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing; Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Collaborative Innovation Center of Hematology, Soochow University, Suzhou; Collaborative Innovation Center of Hematology, Soochow University, Suzhou.
authors.forename: Suning
authors.initials: S
authors.lastname: Chen
keywords: HHT; leukemia; magnetic Fe3O4 nanoparticles; magnetic nanoparticles