title: RACK1 is required for adipogenesis.
abstract: Adipose tissue plays a critical role in metabolic diseases and the maintenance of energy homeostasis. RACK1 has been identified as an adaptor protein involved in multiple intracellular signal transduction pathways and diseases. However, whether it regulates adipogenesis remains unknown. Here, we reported that RACK1 is expressed in 3T3-L1 cells and murine white adipose tissue and that RACK1 knockdown by shRNA profoundly suppressed adipogenesis by reducing the expression of PPAR-γ and C/EBP-β. Depletion of RACK1 increased β-catenin protein levels and activated Wnt signaling. Furthermore, RACK1 knockdown also suppressed the PI3K-Akt-mTOR-S6K signaling pathway by reducing the PI3K p85α, pAkt T473, and S6K p70. Taken together, these results demonstrate that RACK1 is a novel factor required for adipocyte differentiation by emerging Wnt/β-catenin signaling and PI3K-Akt-mTOR-S6K signaling pathway(s).
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.|Graduate University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.|Biomedical Engineering Research Center, Kunming Medical University, Kunming, China; and.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.|Graduate University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Pianchou
authors.initials: P
authors.lastname: Gongpan
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.|Graduate University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.|Yunnan Key Laboratory of Natural Medicinal Chemistry, Kumming, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China; xiong.wenyong@mail.kib.ac.cn.|Yunnan Key Laboratory of Natural Medicinal Chemistry, Kumming, China.
authors.forename: Wenyong
authors.identifier: http://orcid.org/0000-0001-9174-3667
authors.initials: W
authors.lastname: Xiong
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism; D050156:Adipogenesis / Q000502:physiology*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D051379:Mice; D009479:Neuropeptides / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D000075370:Receptors for Activated C Kinase; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000502:physiology; D051176:beta Catenin / Q000378:metabolism
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D009479:Neuropeptides; D047495:PPAR gamma; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D034741:RNA, Small Interfering; D000075370:Receptors for Activated C Kinase; D058570:TOR Serine-Threonine Kinases; D051176:beta Catenin; C545013:Cebpb protein, mouse; C090294:RACK1 protein, mouse
keywords: 3T3-L1; PI3K-Akt-mTOR-S6K; RACK1; Wnt; adipogenesis