title: Nerve-granular cell communication in the atrium of the snail Achatina achatina occurs via the cardioexcitatory transmitters serotonin and FMRFamide.
abstract: In the present study, the anatomical association and functional interaction between nerve fibres and granular cells in the atrium of the snail Achatina achatina are investigated using a combination of scanning electron microscopy (SEM), pharmacological and immunofluorescence techniques. The SEM studies support a close anatomical association of axons with granular cells and new features of surface morphology are revealed. Pharmacological experiments showed that both serotonin and FMRFamide were able to induce degranulation of granular cells and the release of cysteine-rich atrial secretory protein. Serotonin- and FMRFamide-immunoreactive nerve fibres were observed at variable distances from granular cells, ranging from close contact to distances as far as the diameter of a muscle bundle. These results suggest that serotonin and FMRFamide play a role as paracrine excitatory transmitters in nerve-to-granular cell communication.
authors.affiliation: Department of Cytology and Histology, Saint-Petersburg State University, St. Petersburg, Russia. sergey.v.shabelnikov@gmail.com.|Laboratory of Cell Morphology, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia. sergey.v.shabelnikov@gmail.com.
authors.forename: Sergey
authors.initials: S
authors.lastname: Shabelnikov
mesh_terms: D000818:Animals; D002450:Cell Communication; D019835:FMRFamide / Q000378:metabolism*; D006325:Heart Atria / Q000166:cytology* / Q000294:innervation; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D018377:Neurotransmitter Agents / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D012908:Snails / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D018377:Neurotransmitter Agents; D012701:Serotonin; D019835:FMRFamide
keywords: Heart; Mollusca; Nervous system; Paracrine transmission; Spirochete