title: Detection of high-risk human papillomavirus infection in tonsillar specimens using 2 commercially available assays.
abstract: THE OBJECTIVE OF THE STUDY IS TO DETERMINE THE PREVALENCE OF HIGH-RISK HUMAN PAPILLOMAVIRUS (HRHPV) INFECTION IN TONSILLAR SWABS AND TISSUE: Patients undergoing tonsillectomy for nonmalignant causes were enrolled. A flocked swab and fresh tissue were collected from the left and right tonsil of each patient. Specimens were tested for hrHPV DNA using the Roche cobas test and for the presence of E6/E7 messenger RNA using the Hologic Aptima hrHPV test. Of the 193 patients enrolled, 129 were in the pediatric group (ages 1-12years; median, 5years), and 64 were in the adult group (ages 13-55; median, 22years). All swab and tissue specimens were negative for hrHPV by both methods. Positive, negative, and internal controls performed as expected. We found a 0% rate of infection indicating that detectable hrHPV infection in tonsillar tissue appears to be uncommon in the children and adults in the population sampled.
authors.affiliation: The Department of Otorhinolaryngology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Cockerill.cara@mayo.edu.
authors.forename: Cara C
authors.initials: CC
authors.lastname: Cockerill
authors.affiliation: The Department of Otorhinolaryngology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Orvidas.laura@mayo.edu.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Orvidas
authors.affiliation: The Department of Otorhinolaryngology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Moore.eric@mayo.edu.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Moore
authors.affiliation: The Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Binnicker.Matthew@mayo.edu.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Binnicker
authors.affiliation: The Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Duresko.brian@mayo.edu.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Duresko
authors.affiliation: The Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Espy.mark@mayo.edu.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Espy
authors.affiliation: The Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Franklincockerill@yahoo.com.
authors.forename: Franklin R
authors.initials: FR
authors.lastname: Cockerill
authors.affiliation: The Department of Otorhinolaryngology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Tombers.nicole@mayo.edu.
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Tombers
authors.affiliation: The Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Pritt.Bobbi@mayo.edu.
authors.forename: Bobbi S
authors.initials: BS
authors.lastname: Pritt
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D014066:Palatine Tonsil / Q000821:virology*; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D015995:Prevalence; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral
keywords: Human papillomavirus; Oropharyngeal cancer; Tonsil