title: Upregulation of lncRNA HNF1A-AS1 promotes cell proliferation and metastasis in osteosarcoma through activation of the Wnt/β-catenin signaling pathway.
abstract: HNF1A-antisense 1 (HNF1A-AS1), a long non-coding RNA (lncRNA), is associated with metastasis and is an independent prognostic factor for lung cancer. Recent studies demonstrated that HNF1A-AS1 play important roles in cacinogenesis. However, the exact effects and molecular mechanisms of HNF1A-AS1 in osteosarcoma (OS) progression is still unclear. In the present study, we found that HNF1A-AS1 was markedly up-regulated in OS tissues compared to their adjacent non-tumor tissues. HNF1A-AS1 expression levels were positively associated with the clinical stage, distant metastasis, and reduced overall survival of OS patients. In addition, knockdown HNF1A-AS1 expression inhibited cell proliferation, metastasis and influences the activity of the Wnt/β-catenin pathway in OS cells. Wnt/β-catenin pathway activator (LiCl) rescued the anticancer effect of knockdown HNF1A-AS1 expression in OS cells. In conclusion, our study demonstrated that HNF1A-AS1 promoted the progression of OS via regulating the activity of the Wnt/β-catenin pathway, indicating that HNF1A-AS1 might be a potential target for the treatment of OS.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Henan, China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Henan, China.
authors.forename: Wengen
authors.initials: W
authors.lastname: Hou
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Henan, China.
authors.forename: Jingang
authors.initials: J
authors.lastname: Tao
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Henan, China.
authors.forename: Yilei
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Henan, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Wan
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Henan, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, Henan, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Xu
keywords: HNF1A-AS1; Osteosarcoma; Wnt/β-catenin pathway; long non-coding RNA