title: Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis.
abstract: Synchronized tissue polarization during regeneration or de novo vascular tissue formation is a plant-specific example of intercellular communication and coordinated development. According to the canalization hypothesis, the plant hormone auxin serves as polarizing signal that mediates directional channel formation underlying the spatio-temporal vasculature patterning. A necessary part of canalization is a positive feedback between auxin signaling and polarity of the intercellular auxin flow. The cellular and molecular mechanisms of this process are still poorly understood, not the least, because of a lack of a suitable model system. We show that the main genetic model plant, Arabidopsis (Arabidopsis thaliana) can be used to study the canalization during vascular cambium regeneration and new vasculature formation. We monitored localized auxin responses, directional auxin-transport channels formation, and establishment of new vascular cambium polarity during regenerative processes after stem wounding. The increased auxin response above and around the wound preceded the formation of PIN1 auxin transporter-marked channels from the primarily homogenous tissue and the transient, gradual changes in PIN1 localization preceded the polarity of newly formed vascular tissue. Thus, Arabidopsis is a useful model for studies of coordinated tissue polarization and vasculature formation after wounding allowing for genetic and mechanistic dissection of the canalization hypothesis.
authors.affiliation: Department of Cell Biology, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, 40-032 Katowice, Jagiellońska 28, Poland.|Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University (MU), CZ-62-500 Brno, Czech Republic.
authors.forename: Ewa
authors.initials: E
authors.lastname: Mazur
authors.affiliation: Institute of Science and Technology (IST), 3400 Klosterneuburg, Austria.
authors.forename: Eva
authors.initials: E
authors.lastname: Benková
authors.affiliation: Institute of Science and Technology (IST), 3400 Klosterneuburg, Austria.
authors.forename: Jiří
authors.initials: J
authors.lastname: Friml
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D058506:Cambium / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism*; D018547:Plant Stems / Q000502:physiology*; D012038:Regeneration
chemical_list: D007210:Indoleacetic Acids