title: Control of human VDAC-2 scaffold dynamics by interfacial tryptophans is position specific.
abstract: Membrane proteins employ specific distribution patterns of amino acids in their tertiary structure for adaptation to their unique bilayer environment. The solvent-bilayer interface, in particular, displays the characteristic 'aromatic belt' that defines the transmembrane region of the protein, and satisfies the amphipathic interfacial environment. Tryptophan-the key residue of this aromatic belt-is known to influence the folding efficiency and stability of a large number of well-studied α-helical and β-barrel membrane proteins. Here, we have used functional and biophysical techniques coupled with simulations, to decipher the contribution of strategically placed four intrinsic tryptophans of the human outer mitochondrial membrane protein, voltage-dependent anion channel isoform-2 (VDAC-2). We show that tryptophans help in maintaining the structural and functional integrity of folded hVDAC-2 barrel in micellar environments. The voltage gating characteristics of hVDAC-2 are affected upon mutation of tryptophans at positions 75, 86 and 221. We observe that Trp-160 and Trp-221 play a crucial role in the folding pathway of the barrel, and once folded, Trp-221 helps stabilize the folded protein in concert with Trp-75 and Trp-160. We further demonstrate that substituting Trp-86 with phenylalanine leads to the formation of stable barrel. We find that the region comprising strand β4 (Trp-86) and β10-14 (Trp-160 and Trp-221) display slower and faster folding kinetics, respectively, providing insight into a possible directional folding of hVDAC-2 from the C-terminus to N-terminus. Our results show that residue selection in a protein during evolution is a balancing compromise between optimum stability, function, and regulating protein turnover inside the cell.
authors.affiliation: Molecular Biophysics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India.
authors.forename: Svetlana Rajkumar
authors.initials: SR
authors.lastname: Maurya
authors.affiliation: Molecular Biophysics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India. Electronic address: maha@iiserb.ac.in.
authors.forename: Radhakrishnan
authors.initials: R
authors.lastname: Mahalakshmi
mesh_terms: D006801:Humans; D007700:Kinetics; D008823:Micelles; D017510:Protein Folding; D055550:Protein Stability; D013816:Thermodynamics; D014364:Tryptophan / Q000737:chemistry*; D050996:Voltage-Dependent Anion Channel 2 / Q000737:chemistry*
chemical_list: D008823:Micelles; C494765:VDAC2 protein, human; D050996:Voltage-Dependent Anion Channel 2; D014364:Tryptophan
keywords: Kinetics; Stability; Thermodynamics; Tryptophans; VDAC-2