title: Characterization and Comparative Genomic Analyses of Pseudomonas aeruginosa Phage PaoP5: New Members Assigned to PAK_P1-like Viruses.
abstract: As a potential alternative to antibiotics, phages can be used to treat multi-drug resistant bacteria. As such, the biological characteristics of phages should be investigated to utilize them as effective antimicrobial agents. In this study, phage PaoP5, a lytic virus that infects Pseudomonas aeruginosa PAO1, was isolated and genomically characterized. PaoP5 comprises an icosahedral head with an apex diameter of 69 nm and a contractile tail with a length of 120 nm. The PaoP5 genome is a linear dsDNA molecule containing 93,464 base pairs (bp) with 49.51% G + C content of 11 tRNA genes and a 1,200 bp terminal redundancy. A total of 176 protein-coding genes were predicted in the PaoP5 genome. Nine PaoP5 structural proteins were identified. Three hypothetical proteins were determined as structural. Comparative genomic analyses revealed that seven new Pseudomonas phages, namely, PaoP5, K8, C11, vB_PaeM_C2-10_Ab02, vB_PaeM_C2-10_Ab08, vB_PaeM_C2-10_Ab10, and vB_PaeM_C2-10_Ab15, were similar to PAK_P1-like viruses. Phylogenetic and pan-genome analyses suggested that the new phages should be assigned to PAK_P1-like viruses, which possess approximately 100 core genes and 150 accessory genes. This work presents a detailed and comparative analysis of PaoP5 to enhance our understanding of phage biology.
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Mengyu
authors.initials: M
authors.lastname: Shen
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Le
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Yinling
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Xiancai
authors.initials: X
authors.lastname: Rao
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Hu
authors.affiliation: Department of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Lu