title: Portable microsystem integrates multifunctional dielectrophoresis manipulations and a surface stress biosensor to detect red blood cells for hemolytic anemia.
abstract: Hemolytic anemia intensity has been suggested as a vital factor for the growth of certain clinical complications of sickle cell disease. However, there is no effective and rapid diagnostic method. As a powerful platform for bio-particles testing, biosensors integrated with microfluidics offer great potential for a new generation of portable point of care systems. In this paper, we describe a novel portable microsystem consisting of a multifunctional dielectrophoresis manipulations (MDM) device and a surface stress biosensor to separate and detect red blood cells (RBCs) for diagnosis of hemolytic anemia. The peripheral circuit to power the interdigitated electrode array of the MDM device and the surface stress biosensor test platform were integrated into a portable signal system. The MDM includes a preparing region, a focusing region, and a sorting region. Simulation and experimental results show the RBCs trajectories when they are subjected to the positive DEP force, allowing the successful sorting of living/dead RBCs. Separated RBCs are then transported to the biosensor and the capacitance values resulting from the variation of surface stress were measured. The diagnosis of hemolytic anemia can be realized by detecting RBCs and the portable microsystem provides the assessment to the hemolytic anemia patient.
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education &College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Shengbo
authors.initials: S
authors.lastname: Sang
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education &College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Feng
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education &College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Aoqun
authors.initials: A
authors.lastname: Jian
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education &College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Huiming
authors.initials: H
authors.lastname: Li
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education &College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Ji
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education &College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Duan
authors.affiliation: MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education &College of Information Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
authors.forename: Wendong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Shanxi Academy of Medical Sciences &Shanxi Dayi Hospital, Taiyuan, 030032 Shanxi, P.R China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
mesh_terms: D000743:Anemia, Hemolytic / Q000097:blood* / Q000175:diagnosis*; D015374:Biosensing Techniques; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D004912:Erythrocytes / Q000473:pathology*; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*