title: Agaricus blazei extract attenuates rotenone-induced apoptosis through its mitochondrial protective and antioxidant properties in SH-SY5Y neuroblastoma cells.
abstract: The present study was aimed to find out the effect of Agaricus blazei mushroom extract against rotenone-induced cellular model. SH-SY5Y neuroblastoma cells are divided into four experimental groups (control, rotenone (100 nM), A. blazei (5 μg/ml) + rotenone (100 nM), and A. blazei alone treated) based on MTT assay, cells were allowed to measure the ROS, TBARS levels, and antioxidants activities. Finally, mitochondrial transmembrane potential (MMP) and expressions of apoptotic proteins were also analyzed. Pre-treatment with A. blazei significantly enhanced cell viability, attenuated rotenone-induced ROS, MMP, and apoptosis. Our results indicated that anti-apoptotic properties of this natural compound due to its antioxidant and mitochondrial protective function protect rotenone-induced cytotoxicity. Therefore, it may be concluded that A. blazei can be further developed as a promising drug for the treatment of Parkinson's disease (PD).
authors.affiliation: a Department of Anatomy , Bharath University , Selaiyur, Chennai , Tamil Nadu 600073 , India.
authors.forename: Veerappan
authors.initials: V
authors.lastname: Venkatesh Gobi
authors.affiliation: b Department of Anatomy , Velammal Medical College and Hospital , Madurai , Tamil Nadu 625009 , India.
authors.forename: Srinivasagam
authors.initials: S
authors.lastname: Rajasankar
authors.affiliation: a Department of Anatomy , Bharath University , Selaiyur, Chennai , Tamil Nadu 600073 , India.
authors.forename: Muthu
authors.initials: M
authors.lastname: Ramkumar
authors.affiliation: c Department of Biochemistry and Biotechnology , Annamalai University , Annamalainagar , Tamil Nadu 608002 , India.
authors.forename: Chinnasamy
authors.initials: C
authors.lastname: Dhanalakshmi
authors.affiliation: c Department of Biochemistry and Biotechnology , Annamalai University , Annamalainagar , Tamil Nadu 608002 , India.
authors.forename: Thamilarasan
authors.initials: T
authors.lastname: Manivasagam
authors.affiliation: c Department of Biochemistry and Biotechnology , Annamalai University , Annamalainagar , Tamil Nadu 608002 , India.
authors.forename: Arokiasamy
authors.initials: A
authors.lastname: Justin Thenmozhi
authors.affiliation: d Department of Food Science and Nutrition, CAMS , Sultan Qaboos University , Muscat , Oman.|e Ageing and Dementia Research Group , Sultan Qaboos University , Muscat , Oman.|f Food and Brain Research Foundation , Chennai , Tamil Nadu 600094 , India.
authors.forename: Musthafa Mohamed
authors.initials: MM
authors.lastname: Essa
authors.affiliation: g Department of Radiology , Sri Lakshminarayana Institute of Medical Sciences , Puducherry , India.
authors.forename: Ranganathan
authors.initials: R
authors.lastname: Chidambaram
mesh_terms: D000363:Agaricales / Q000737:chemistry; D000364:Agaricus / Q000737:chemistry*; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009447:Neuroblastoma / Q000188:drug therapy; D018384:Oxidative Stress / Q000187:drug effects; D010300:Parkinson Disease; D017382:Reactive Oxygen Species / Q000378:metabolism; D012402:Rotenone / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D051017:Apoptosis Regulatory Proteins; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D012402:Rotenone; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: A. blazei; Apoptosis; Mitochondria membrane potential; Oxidative stress; Rotenone