title: Feasibility and kinetics of CD34+hematopoietic progenitor cell mobilization in response to a single administration of docetaxel chemotherapy and pegfilgrastim in a contemporary cohort of patients with metastatic breast cancer.
abstract: OBJECTIVE
  Autologous hematopoietic stem cell transplantation (auto-HSCT) remains an experimental therapy for metastatic breast cancer (MBC) and there is no established protocol for cluster of differentiation 34+(CD34+) hematopoietic progenitor cell (HPC) mobilization with historic studies using growth factors with or without chemotherapy. This study describes the feasibility and kinetics of CD34+HPC mobilization following a single administration of docetaxel and the pegylated form of recombinant human granulocyte colony-stimulating factor analogue filgrastim (pegfilgrastim).
  
  
  METHODS
  The study design was serial measurement of peripheral blood CD34+HPC in patients with MBC following a single administration of intravenous (IV) docetaxel 100 mg/m2on day 1 and subcutaneous (SC) pegfilgrastim 6 mg on day 2.
  
  
  RESULTS
  Eight patients with MBC were enrolled. The median age was 56 years (range 51-75 years). All patients had human epidermal growth factor receptor 2 (HER2) negative disease and either hormone refractory or negative disease. Three patients had bone only disease, four had visceral organ disease with or without bone involvement and one had locally unresectable disease only. All patients had prior therapy for early-advanced stage disease and prior therapy for MBC included seven patients receiving at least one line of hormone therapy and three having palliative chemotherapy. Six patients recorded a rise in the CD34+count greater than 20 cells/μL. The median peak level was 40.2 cells/μL (standard deviation = 28.7) occurring on day 9 and with an average duration of 4 days. Overall, treatment was well tolerated with manageable side-effects.
  
  
  CONCLUSIONS
  The single administration of docetaxel and pegfilgrastim was effective in mobilization of CD34+HPC and peak levels followed a predictable course. This approach needs validation in prospective studies by preparation of auto-HSCT by leukapheresis and quantification of total CD34+HPC yields.
authors.affiliation: Cancer Clinical Trials Unit, Royal Adelaide Hospital, Adelaide, SA, Australia.
authors.forename: Harminder
authors.initials: H
authors.lastname: Takhar
authors.affiliation: Cancer Clinical Trials Unit, Royal Adelaide Hospital, Adelaide, SA, Australia.
authors.forename: Anna Rachelle
authors.initials: AR
authors.lastname: Mislang
authors.affiliation: Cancer Clinical Trials Unit, Royal Adelaide Hospital, Adelaide, SA, Australia.
authors.forename: Nimit
authors.initials: N
authors.lastname: Singhal
authors.affiliation: Cancer Clinical Trials Unit, Royal Adelaide Hospital, Adelaide, SA, Australia.|Centre for Cancer Biology, SA Pathology and University of South Australia, Adelaide, SA, Australia.|Discipline of Medicine, University of Adelaide, Adelaide, SA, Australia.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Brown
mesh_terms: D000368:Aged; D018952:Antigens, CD34; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000188:drug therapy*; D000077143:Docetaxel; D005240:Feasibility Studies; D005260:Female; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006801:Humans; D008875:Middle Aged; D011092:Polyethylene Glycols; D011446:Prospective Studies; D011994:Recombinant Proteins / Q000627:therapeutic use; D043823:Taxoids / Q000627:therapeutic use*
chemical_list: D018952:Antigens, CD34; D011994:Recombinant Proteins; D043823:Taxoids; D016179:Granulocyte Colony-Stimulating Factor; D000077143:Docetaxel; C455861:pegfilgrastim; D011092:Polyethylene Glycols; D000069585:Filgrastim
keywords: CD34; antineoplastic agents; breast neoplasms; granulocyte colony-stimulating factor; hematopoietic progenitor cell mobilization