title: OsDi19-4 acts downstream of OsCDPK14 to positively regulate ABA response in rice.
abstract: The drought-induced 19 protein family consists of several atypical Cys2/His2-type zinc finger proteins in plants and plays an important role in abiotic stress. In this study, we found that overexpressing OsDi19-4 in rice altered the expression of a series of abscisic acid (ABA)-responsive genes, resulting in strong ABA-hypersensitive phenotypes including ABA-induced seed germination inhibition, early seedling growth inhibition and stomatal closure. On the contrary, OsDi19-4 knockdown lines were less sensitive to ABA. Additionally, OsCDPK14 was identified to interact with OsDi19-4 and be responsible for the phosphorylation of OsDi19-4, and the phosphorylation of OsDi19-4 was further enhanced after the treatment of ABA. Apart from these, OsDi19-4 was shown to directly bind to the promoters of OsASPG1 and OsNAC18 genes, two ABA-responsive genes, and regulate their expression. Transient expression assays confirmed the direct regulation role of OsDi19-4, and the regulation was further enhanced by the increased phosphorylation of OsDi19-4 after the treatment of ABA. Taken together, these data demonstrate that OsDi19-4 acts downstream of OsCDPK14 to positively regulate ABA response by modulating the expression of ABA-responsive genes in rice.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Changchun
authors.initials: C
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Shanglin
authors.initials: S
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.|Key Laboratory for Research and Utilization of Heterosis in Indica Rice, the Ministry of Agriculture, The Yangtze River Valley Hybrid Rice Collaboration & Innovation Center, Wuhan University, Wuhan, 430072, China.
authors.forename: Yingguo
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.|Key Laboratory for Research and Utilization of Heterosis in Indica Rice, the Ministry of Agriculture, The Yangtze River Valley Hybrid Rice Collaboration & Innovation Center, Wuhan University, Wuhan, 430072, China.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Huang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D055785:Gene Knockdown Techniques; D018525:Germination; D047468:Immunoprecipitation; D012275:Oryza / Q000378:metabolism / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism / Q000502:physiology*; D010940:Plant Proteins / Q000378:metabolism / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D012639:Seeds / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers / Q000502:physiology
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; D000040:Abscisic Acid
keywords: ABA signal; CDPK; Di19; phosphorylation; stress responses