title: Krüppel-like factor 2 suppresses growth and invasion of gastric cancer cells in vitro and in vivo.
abstract: Krüppel-like factor 2 (KLF2), a novel tumor-suppressor gene, is implicated in diverse cellular processes, including cell growth, apoptosis, and invasion. However, the role and action mechanisms of KLF2 in gastric cancer (GC) need be further elucidated. The expression of KLF2 was investigated by immunohistochemical assay in human GC tissues, and lentivirus-mediated KLF2 overexpression was transfected into GC cells (AGS and HGC-27) for assessing cell proliferation and invasion, respectively indicated by MTT and Transwell assays. Subcutaneous GC tumor models were constructed for estimating tumor growth in vivo. As a result, the expression level of KLF2 was decreased in GC tissues compared with the para-carcinoma tissues (31.03% vs 53.45%, P=0.035), and negatively correlated with the lymph node metastasis in GC patients (P=0.02). Moreover, overexpression of KLF2 inhibited the cell proliferation and invasive potential and downregulated the protein expression of PCNA, Bcl-2 and MMP-9 in GC cells. The result in vivo showed KLF2 overexpression reduced the xenograft tumor growth. In conclusion, our findings indicate that KLF2 may function as a tumor suppressor involved in the progression of human GC.
authors.affiliation: Gastroenterology Department and Endoscopy Center of Fudan University Affiliated Huashan Hospital North Hospital, Shanghai, China.
authors.forename: Q Q
authors.initials: QQ
authors.lastname: Mao
authors.affiliation: Gastroenterology Department and Endoscopy Center of Fudan University Affiliated Huashan Hospital North Hospital, Shanghai, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: Gastroenterology Department and Endoscopy Center of Fudan University Affiliated Huashan Hospital North Hospital, Shanghai, China.
authors.forename: L
authors.initials: L
authors.lastname: Dong
authors.affiliation: Gastroenterology Department and Endoscopy Center of Fudan University Affiliated Huashan Hospital North Hospital, Shanghai, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Gastroenterology Department and Endoscopy Center of Fudan University Affiliated Huashan Hospital North Hospital, Shanghai, China.
authors.forename: X
authors.initials: X
authors.lastname: Sun
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D064593:Heterografts; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D016086:Lentivirus; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: C096161:KLF2 protein, human; D051741:Kruppel-Like Transcription Factors; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins