title: Sequence variation in two genes determines the efficacy of transmission of citrus tristeza virus by the brown citrus aphid.
abstract: Vector transmission is an important part of the viral infection cycle, yet for many viruses little is known about this process, or how viral sequence variation affects transmission efficacy. Here we examined the effect of substituting genes from the highly transmissible FS577 isolate of citrus tristeza virus (CTV) in to the poorly transmissible T36-based infectious clone. We found that introducing p65 or p61 sequences from FS577 significantly increased transmission efficacy. Interestingly, replacement of both genes produced a greater increase than either gene alone, suggesting that CTV transmission requires the concerted action of co-evolved p65 and p61 proteins.
authors.affiliation: Department of Plant Pathology, University of Florida, 700 Experiment Station Road, Lake Alfred, Florida, 33850, USA. sjharper@ufl.edu.
authors.forename: S J
authors.initials: SJ
authors.lastname: Harper
authors.affiliation: Department of Plant Pathology, University of Florida, 700 Experiment Station Road, Lake Alfred, Florida, 33850, USA.
authors.forename: N
authors.initials: N
authors.lastname: Killiny
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, and Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
authors.forename: S
authors.initials: S
authors.lastname: Tatineni
authors.affiliation: Department of Plant Pathology, University of Florida, 700 Experiment Station Road, Lake Alfred, Florida, 33850, USA.
authors.forename: S
authors.initials: S
authors.lastname: Gowda
authors.affiliation: Department of Plant Pathology, University of Florida, 700 Experiment Station Road, Lake Alfred, Florida, 33850, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Cowell
authors.affiliation: Department of Plant Pathology, University of Florida, 700 Experiment Station Road, Lake Alfred, Florida, 33850, USA.
authors.forename: T
authors.initials: T
authors.lastname: Shilts
authors.affiliation: Department of Plant Pathology, University of Florida, 700 Experiment Station Road, Lake Alfred, Florida, 33850, USA.
authors.forename: W O
authors.initials: WO
authors.lastname: Dawson
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology*; D002957:Citrus / Q000821:virology*; D017790:Closterovirus / Q000235:genetics*; D014644:Genetic Variation; D007303:Insect Vectors; D010935:Plant Diseases / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins