title: NtPHYB1K326, a homologous gene of Arabidopsis PHYB, positively regulates the content of phenolic compounds in tobacco.
abstract: Polyphenols are important secondary metabolites and bioactive compounds in plants. Light is a vital abiotic factor that greatly impacts the content of polyphenols in plants. In spite of their importance the mechanism of polyphenol regulation still remains unknown in tobacco. A phytochrome B homolog, NtPHYB1K326, was isolated from Nicotiana tabacum cv. K326 to investigate the role of light receptors in the regulation of polyphenol metabolism in tobacco leaves. Furthermore, role of NtPHYB1K326in polyphenol metabolism was analyzed by over-expression and RNAi-silencing approaches. Consistent and complemented results indicated involvement of NtPHYB1K326in the regulation of polyphenol metabolism in tobacco leaves. Moreover, high levels of NtPHYB1K326transcripts favor the accumulation of chlorogenic acid and its isomers, the key polyphenol component in tobacco leaves. Transcriptome analysis was also carried out for exploring the regulation mechanism of NtPHYB1K326in the polyphenol metabolism. Compared with WT, 1665 and 1421 differentially-expressed genes were found in NtPHYB1K326-GFP and NtPHYB1K326-RNAi transgenic lines, respectively. Among these, about 30 genes were related to phenylpropanoid pathway, which is predominantly involved in synthesis of polyphenols. Further evidences from quantitative RT-PCR confirmed that NtPHYB1K326may control phenylpropanoid pathway through regulating the transcription of PAL4 (phenylalanine ammonialyase 4), 4CL1 (4-coumarate:coenzyme A ligase 1) and COMT (caffeic acid 3-O-methyltransferase) genes.
authors.affiliation: CNTC Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, 29 Longtanba Road, Guanshanhu District, Guiyang, 550081, China. Electronic address: zhaojiehong@126.com.
authors.forename: Jiehong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Basic Medical Science, Guiyang College of Traditional Chinese Medicine, Huaxi District, 550025, Guiyang, China. Electronic address: hanjie2008@126.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Han
authors.affiliation: CNTC Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, 29 Longtanba Road, Guanshanhu District, Guiyang, 550081, China. Electronic address: zhechen361023@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: CNTC Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, 29 Longtanba Road, Guanshanhu District, Guiyang, 550081, China. Electronic address: 297495501@qq.com.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Li
authors.affiliation: CNTC Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, 29 Longtanba Road, Guanshanhu District, Guiyang, 550081, China. Electronic address: yujingbio@126.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.affiliation: CNTC Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, 29 Longtanba Road, Guanshanhu District, Guiyang, 550081, China. Electronic address: shzyuu@126.com.
authors.forename: Shizhou
authors.initials: S
authors.lastname: Yu
authors.affiliation: CNTC Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, 29 Longtanba Road, Guanshanhu District, Guiyang, 550081, China. Electronic address: yshguo@126.com.
authors.forename: Yushuang
authors.initials: Y
authors.lastname: Guo
authors.affiliation: MOA Key Lab of Soybean Biology (Beijing), National Key Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Haidian District, Beijing, 100081, China. Electronic address: fufu19cn@163.com.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Fu
authors.affiliation: MOA Key Lab of Soybean Biology (Beijing), National Key Facility of Crop Gene Resource and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Haidian District, Beijing, 100081, China. Electronic address: zhangxiaomei@caas.cn.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002726:Chlorogenic Acid / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D015151:Immunoblotting; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D051939:Phytochrome B / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C497952:PHYB protein, Arabidopsis; D010636:Phenols; D010940:Plant Proteins; D051939:Phytochrome B; D002726:Chlorogenic Acid; D008780:Methyltransferases; C019608:caffeate O-methyltransferase; D010650:Phenylalanine Ammonia-Lyase; D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase
keywords: Phytochrome B; Polyphenols; Secondary metabolism; Tobacco; Transcriptome