title: Generation of Human Antibodies Against a Specific Peptide: a Novel Strategy Based on Human Cells.
abstract: We developed a novel platform to produce fully human antibodies that could be used as tools in cancer research. This study was based on maturation of B-cells into plasma cells and the subsequent secretion of fully human antibodies against a 9-mer peptide.
  
  
  
  A peptide was introduced to dendritic cells, followed by co-cultivation with cluster of differentiation 4 (CD4+) cells from healthy donors and CD19(+) cells from patients with cancer. In order to detect plasma cells, we used microscopy, flow cytometry, enzyme-linked immunosorbent assays (ELISAs), and western blotting.
  
  
  
  Oval-shaped cells with eccentric nuclei were found which expressed high levels of CD27 (up to 73%) and CD138 (up to 31%). An antibody against the selected peptide was detected in the culture supernatants of all samples according to ELISA and western blot analyses.
  
  
  
  This platform successfully generated fully human antibodies against a specific epitope.
authors.affiliation: Research Genetic Cancer Centre Group, Florina, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Toloudi
authors.affiliation: Research Genetic Cancer Centre Group, Florina, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Papadimitriou
authors.affiliation: Research Genetic Cancer Centre Group, Florina, Greece.
authors.forename: Katerina
authors.initials: K
authors.lastname: Pantopikou
authors.affiliation: Research Genetic Cancer Centre Group, Florina, Greece papasotiriou.ioannis@rgcc-genlab.com office@rgcc-genlab.com.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Papasotiriou
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D018941:Antigens, CD19 / Q000378:metabolism; D001943:Breast Neoplasms / Q000276:immunology* / Q000628:therapy*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000276:immunology*; D053668:Syndecan-1 / Q000378:metabolism; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D018941:Antigens, CD19; C000604811:CD19 molecule, human; D000939:Epitopes; D010455:Peptides; C508241:SDC1 protein, human; D053668:Syndecan-1; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
keywords: Monoclonal antibodies; breast cancer