title: Comparative assessment of the effectiveness of different cleaning methods on the growth of Candida albicans over acrylic surface.
abstract: BACKGROUND
  This study evaluated the efficacy of denture adhesive, cleanser, chlorhexidine, and brushing against Candida albicans biofilm developed on an acrylic surface and predicted the most effective, simple, and inexpensive way to maintain denture health, thereby preventing denture stomatitis.
  
  
  OBJECTIVE
  To find the best possible method for maintaining denture hygiene.
  
  
  METHODS
  This retrospective analysis was conducted in the Guru Nanak Institute of Dental Sciences and Research, Kolkata, and this in vitro study was designed to minimize denture stomatitis among denture wearing population.
  
  
  METHODS
  Sixty acrylic discs of equal dimensions after exposure to C. albicans were treated for a duration of 24 h with denture adhesive, cleanser, 0.2% chlorhexidine individually, or in combinations simulating clinical conditions dividing in six groups, ten samples each (n = 10).
  
  
  METHODS
  After treatment, colony count was evaluated and statistically analyzed by post hoc Tukey's test and Dunnett's test to determine the most effective way of prevention.
  
  
  RESULTS
  The statistical post hoc analysis (Tukey's test and Dunnett's test) showed high significance (P < 0.0001). The group treated with adhesive showed high fungal growth compared to the control group, whereas chlorhexidine showed high potency to prevent C. albicans, whereas adhesive increased the adhesion of C. albicans to acrylic surface.
  
  
  CONCLUSIONS
  Denture adhesive increases the adherence of C. albicans to denture surface. Other cleaning chemicals such as cleanser and chlorhexidine decrease the adherence. Moreover, among the all denture cleaning protocol, chlorhexidine drastically inhibit the adherence, as well as growth of C. albicans over denture surface.
authors.affiliation: Department of Prosthodontics and Crown & Bridge, Guru Nanak Institute of Dental Sciences and Research, Kolkata, West Bengal, India.
authors.forename: Subhajit
authors.initials: S
authors.lastname: Gantait
authors.affiliation: Department of Prosthodontics and Crown & Bridge, Guru Nanak Institute of Dental Sciences and Research, Kolkata, West Bengal, India.
authors.forename: Jayanta
authors.initials: J
authors.lastname: Bhattacharyya
authors.affiliation: Department of Prosthodontics and Crown & Bridge, Guru Nanak Institute of Dental Sciences and Research, Kolkata, West Bengal, India.
authors.forename: Samiran
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Microbiology, Guru Nanak Institute of Dental Sciences and Research, Kolkata, West Bengal, India.
authors.forename: Shibendu
authors.initials: S
authors.lastname: Biswas
authors.affiliation: Department of Microbiology, Guru Nanak Institute of Dental Sciences and Research, Kolkata, West Bengal, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Ghati
authors.affiliation: Department of Prosthodontics and Crown & Bridge, Guru Nanak Institute of Dental Sciences and Research, Kolkata, West Bengal, India.
authors.forename: Soumitra
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Prosthodontics and Crown & Bridge, Guru Nanak Institute of Dental Sciences and Research, Kolkata, West Bengal, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Goel
keywords: Candida albicans; chlorhexidine; denture adhesive; denture cleanser; denture stomatitis