title: The pentose phosphate pathway leads to enhanced succinic acid flux in biofilms of wild-type Actinobacillus succinogenes.
abstract: Increased pentose phosphate pathway flux, relative to total substrate uptake flux, is shown to enhance succinic acid (SA) yields under continuous, non-growth conditions of Actinobacillus succinogenes biofilms. Separate fermentations of glucose and xylose were conducted in a custom, continuous biofilm reactor at four different dilution rates. Glucose-6-phosphate dehydrogenase assays were performed on cell extracts derived from in situ removal of biofilm at each steady state. The results of the assays were coupled to a kinetic model that revealed an increase in oxidative pentose phosphate pathway (OPPP) flux relative to total substrate flux with increasing SA titre, for both substrates. Furthermore, applying metabolite concentration data to metabolic flux models that include the OPPP revealed similar flux relationships to those observed in the experimental kinetic analysis. A relative increase in OPPP flux produces additional reduction power that enables increased flux through the reductive branch of the TCA cycle, leading to increased SA yields, reduced by-product formation and complete closure of the overall redox balance.
authors.affiliation: Department of Chemical Engineering, University of Pretoria, Lynnwood Road, Hatfield, Private Bag X20, Pretoria, 0002, South Africa.
authors.forename: Michael F A
authors.initials: MF
authors.lastname: Bradfield
authors.affiliation: Department of Chemical Engineering, University of Pretoria, Lynnwood Road, Hatfield, Private Bag X20, Pretoria, 0002, South Africa. willie.nicol@up.ac.za.
authors.forename: Willie
authors.initials: W
authors.lastname: Nicol
mesh_terms: D000188:Actinobacillus / Q000378:metabolism / Q000502:physiology*; D018441:Biofilms; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D064688:Metabolic Flux Analysis; D010427:Pentose Phosphate Pathway; D019802:Succinic Acid / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
chemical_list: D014994:Xylose; D019802:Succinic Acid; D005954:Glucosephosphate Dehydrogenase; D005947:Glucose
keywords: Actinobacillus succinogenes; Biofilm; Glucose-6-phosphate dehydrogenase; Metabolic flux analysis; Pentose phosphate pathway; Succinic acid