title: Sequestration of Proteins by Fatty Acid Coacervates for Their Encapsulation within Vesicles.
abstract: Encapsulating biological materials in lipid vesicles is of interest for mimicking cells; however, except in some particular cases, such processes do not occur spontaneously. Herein, we developed a simple and robust method for encapsulating proteins in fatty acid vesicles in high yields. Fatty acid based, membrane-free coacervates spontaneously sequester proteins and can reversibly form membranous vesicles upon varying the pH value, the precrowding feature in coacervates allowing for protein encapsulation within vesicles. We then produced enzyme-enriched vesicles and show that enzymatic reactions can occur in these micrometric capsules. This work could be of interest in the field of synthetic biology for building microreactors.
authors.affiliation: UMR 1332, biologie et pathologie du fruit, INRA, centre de Bordeaux, 33883, Villenave O'Ornon, France.
authors.forename: David
authors.initials: D
authors.lastname: Garenne
authors.affiliation: UMR 1332, biologie et pathologie du fruit, INRA, centre de Bordeaux, 33883, Villenave O'Ornon, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Beven
authors.affiliation: Université de Bordeaux, Centre de Recherche Paul-Pascal, CNRS, av. A. Schweitzer, 33600, Pessac, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Navailles
authors.affiliation: Université de Bordeaux, Centre de Recherche Paul-Pascal, CNRS, av. A. Schweitzer, 33600, Pessac, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Nallet
authors.affiliation: Institute of Chemistry and Biology of Membranes and Nano-objects, UMR 5248, CNRS, université de Bordeaux, Institut polytechnique Bordeaux, 33600, Pessac, France.
authors.forename: Erick J
authors.initials: EJ
authors.lastname: Dufourc
authors.affiliation: UMR 1332, biologie et pathologie du fruit, INRA, centre de Bordeaux, 33883, Villenave O'Ornon, France. jean-paul.douliez@bordeaux.inra.fr.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Douliez
keywords: coacervates; encapsulation; microreactors; proteins; vesicles