title: Mepivacaine attenuates vasodilation induced by ATP-sensitive potassium channels in rat aorta.
abstract: The goal of this in vitro study was to investigate the effect of mepivacaine on vasodilation induced by the ATP-sensitive potassium (KATP) channel opener levcromakalim in isolated endothelium-denuded rat aortas. The effects of mepivacaine and the KATPchannel inhibitor glibenclamide, alone or in combination, on levcromakalim-induced vasodilation were assessed in the isolated aortas. The effects of mepivacaine or combined treatment with a protein kinase C (PKC) inhibitor, GF109203X, and mepivacaine on this vasodilation were also investigated. Levcromakalim concentration-response curves were generated for isolated aortas precontracted with phenylephrine or a PKC activator, phorbol 12,13-dibutyrate (PDBu). Further, the effects of mepivacaine and glibenclamide on levcromakalim-induced hyperpolarization were assessed in rat aortic vascular smooth muscle cells. Mepivacaine attenuated levcromakalim-induced vasodilation, whereas it had no effect on this vasodilation in isolated aortas pretreated with glibenclamide. Combined treatment with GF109203X and mepivacaine enhanced levcromakalim-induced vasodilation compared with pretreatment with mepivacaine alone. This vasodilation was attenuated in aortas precontracted with PDBu compared with those precontracted with phenylephrine. Mepivacaine and glibenclamide, alone or in combination, attenuated levcromakalim-induced membrane hyperpolarization. Taken together, these results suggest that mepivacaine attenuates vasodilation induced by KATPchannels, which appears to be partly mediated by PKC.
authors.affiliation: a Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University, Biomedical Research Institute, Pusan National University Hospital, 179 Gudeok-ro, Seo-gu, Busan-si, 602-739, Republic of Korea.
authors.forename: Jiseok
authors.initials: J
authors.lastname: Baik
authors.affiliation: b Department of Anesthesiology and Pain Medicine, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Jinju-si, 52727, Republic of Korea.
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Ok
authors.affiliation: c Department of Physiology, Gyeongsang National University School of Medicine, Jinju-si, 52727, Republic of Korea.
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: c Department of Physiology, Gyeongsang National University School of Medicine, Jinju-si, 52727, Republic of Korea.
authors.forename: Dawon
authors.initials: D
authors.lastname: Kang
authors.affiliation: a Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University, Biomedical Research Institute, Pusan National University Hospital, 179 Gudeok-ro, Seo-gu, Busan-si, 602-739, Republic of Korea.
authors.forename: Jeong-Min
authors.initials: JM
authors.lastname: Hong
authors.affiliation: b Department of Anesthesiology and Pain Medicine, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Jinju-si, 52727, Republic of Korea.
authors.forename: Il-Woo
authors.initials: IW
authors.lastname: Shin
authors.affiliation: b Department of Anesthesiology and Pain Medicine, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Jinju-si, 52727, Republic of Korea.
authors.forename: Heon Keun
authors.initials: HK
authors.lastname: Lee
authors.affiliation: b Department of Anesthesiology and Pain Medicine, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Jinju-si, 52727, Republic of Korea.
authors.forename: Young-Kyun
authors.initials: YK
authors.lastname: Chung
authors.affiliation: d Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Jinju-si, 52727, Republic of Korea.
authors.forename: Youngil
authors.initials: Y
authors.lastname: Cho
authors.affiliation: d Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Jinju-si, 52727, Republic of Korea.
authors.forename: Soo Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: d Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Jinju-si, 52727, Republic of Korea.
authors.forename: Sebin
authors.initials: S
authors.lastname: Kang
authors.affiliation: b Department of Anesthesiology and Pain Medicine, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Jinju-si, 52727, Republic of Korea.|e Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Ju-Tae
authors.initials: JT
authors.lastname: Sohn
keywords: GF109203X; KATP channel; aorta; aorte; canal KATP; glibenclamide; levcromakalim; mepivacaine; mépivacaïne; protein kinase C; protéine kinase C