title: Manganese binding to antioxidant peptides involved in extreme radiation resistance in Deinococcus radiodurans.
abstract: A decapeptide, DEHGTAVMLK (DP1), and its random scrambled version, THMVLAKGED (DP2), have been studied for their interactions with manganese. The amino acid composition of the peptides was selected to include the majority of the most prevalent amino acids present in a Deinococcus radiodurans bacterium cell-free extract that contains components capable of conferring extreme resistance to ionizing radiation. The extract appears to be rich in Mn(II) complexes which seem to be responsible for protecting proteins from Reactive Oxygen Species damage. We focused our attention on the interaction of the decapeptides with Mn(II) ion with the aim of obtaining information on the possible complexes formed, by using NMR, EPR, and ESI-MS techniques.
authors.affiliation: Department of Chemistry and Pharmacy, University of Sassari, Italy. Electronic address: peana@uniss.it.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Peana
authors.affiliation: Department of Chemistry and Pharmacy, University of Sassari, Italy.
authors.forename: Serenella
authors.initials: S
authors.lastname: Medici
authors.affiliation: STS on contract to Department of Aeromedical Research, United States Air Force School of Aerospace Medicine, USA.
authors.forename: Heather A
authors.initials: HA
authors.lastname: Pangburn
authors.affiliation: Air Force Research Laboratory, 711th HPW/RHXBC, Wright Patterson Air Force Base, OH 45433, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Lamkin
authors.affiliation: Faculty of Chemistry, University of Wroclaw, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Ostrowska
authors.affiliation: Faculty of Chemistry, University of Wroclaw, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Gumienna-Kontecka
authors.affiliation: Department of Chemistry and Pharmacy, University of Sassari, Italy. Electronic address: zoroddu@uniss.it.
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Zoroddu
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D034301:Deinococcus / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D011836:Radiation Tolerance
chemical_list: D000975:Antioxidants; D009842:Oligopeptides; D008345:Manganese
keywords: Deinococcus radiodurans; Manganese; Peptides; Radiation resistance