title: Molecular characterization and immunological response analysis of toll-like receptors from the blunt snout bream (Megalobrama amblycephala).
abstract: The innate immunity serves as the primary line of defense against pathogen invasion. Toll-like receptors (TLRs) play a pivotal role in the innate immunity by sensing specific pathogen-associated molecular patterns and activating immune responses. In this study, 14 TLRs (TLR1, 2, 3, 4, 5, 7, 8a, 8b, 9, 18, 19, 20, 21 and 22) were identified and characterized in a cyprinid fish Megalobrama amblycephala. A majority of MaTLRs possessed the typical structural features of the TLR protein family: LRR domain, TM region and TIR domain. Phylogenetic analyses confirmed the existence of six TLR families and revealed close relationships with other cyprinid orthologs. All TLRs were constitutively expressed in all eight examined tissues. After Aeromonas hydrophila challenge, the expression profiles of MaTLR mRNAs were analyzed in liver, spleen and head kidney: MaTLR1, 2, 5, 9, 21 and 22 were up-regulated, MaTLR3, 7, 8a, 8b and 19 were down-regulated, and MaTLR4, 18 and 20 expression patterns varied among tissues. These results indicate that most of MaTLRs are likely to be involved in the immune responses against bacterial infection.
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: lairuifang@webmail.hzau.edu.cn.
authors.forename: Rui-Fang
authors.initials: RF
authors.lastname: Lai
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Bio-Transduction Lab, Wuhan Institute of Biotechnology, Wuhan, Hubei Province, 430072, China. Electronic address: ivanjakovlic@yahoo.com.
authors.forename: Ivan
authors.initials: I
authors.lastname: Jakovlić
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: lifegood1986@126.com.
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: 1203248258@qq.com.
authors.forename: Fan-Bin
authors.initials: FB
authors.lastname: Zhan
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: 1161104243@qq.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Wei
authors.affiliation: Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, 41500, China. Electronic address: wangwm@mail.hzau.edu.cn.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Wang
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D016905:Gram-Negative Bacterial Infections / Q000276:immunology*; D060226:Head Kidney / Q000378:metabolism*; D007113:Immunity, Innate / Q000235:genetics; D008099:Liver / Q000378:metabolism*; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000276:immunology; D010802:Phylogeny; D000072417:Protein Domains / Q000235:genetics; D051193:Toll-Like Receptors / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D029941:Fish Proteins; D000069452:Pathogen-Associated Molecular Pattern Molecules; D051193:Toll-Like Receptors
keywords: Aeromonas hydrophila; Expression; Fish; TLR