title: Structural Modulation of Gut Microbiota in Rats with Allergic Bronchial Asthma Treated with Recuperating Lung Decoction.
abstract: OBJECTIVE
  To investigate whether recuperating lung decoction (RLD) can modulate the composition of gut microbiota in rats during asthma treatment.
  
  
  METHODS
  Fifteen Sprague-Dawley rats were divided randomly and equally into control group, model group, dexamethasone (DEX) group, RLD medium-dose group, and RLD high-dose group. The asthma model was established in all groups, except for the control group. The rats in the DEX and RLD groups were treated orally with DEX and RLD, respectively. The rats in the control and model groups were treated orally with 0.9% saline. The intestinal bacterial communities were compared among groups using 16S rRNA gene amplification and 454 pyrosequencing.
  
  
  RESULTS
  The microbial flora differed between the control and model groups, but the flora in the RLD groups was similar to that in the control group. No significant differences were observed between the RLD high-dose and medium-dose groups. RLD treatment resulted in an increase in the level beneficial bacteria in the gut, such as Lactobacillus and Bifidobacterium spp.
  
  
  CONCLUSIONS
  Oral administration of RLD increased the number of intestinal lactic acid-producing bacteria, such as Lactobacillus and Bifidobacterium, in asthma model rats.
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Yan Hua
authors.initials: YH
authors.lastname: Kong
authors.affiliation: Second Department of TCM Pulmonary Disease, The Key Institute of State Administration of Traditional Chinese Medicine (pneumonopathy chronic cough and dyspnea), Beijing Key Laboratory (NO. BZ0321), China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.affiliation: State Key Laboratory for Infectious Diseases Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Beijing 102206, China; Chinese Center for Disease Control and Prevention. Collaborative Innovation Center Diagnosis and Treatment of Infectious Disease, Hangzhou 310003, Zhejiang, China.
authors.forename: Na
authors.initials: N
authors.lastname: Han
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100015, China.
authors.forename: Yuan Yuan
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Nephrology Department, Aviation General Hospital of China Medical University, Beijing 100012, China.
authors.forename: Tong Xin
authors.initials: TX
authors.lastname: Gao
authors.affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100015, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Second Department of TCM Pulmonary Disease, The Key Institute of State Administration of Traditional Chinese Medicine (pneumonopathy chronic cough and dyspnea), Beijing Key Laboratory (NO. BZ0321), China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: You Lin
authors.initials: YL
authors.lastname: Li
mesh_terms: D000818:Animals; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D041981:Gastrointestinal Tract / Q000276:immunology / Q000382:microbiology; D006801:Humans; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Asthma; Gut microbiota; Recuperating lung decoction