title: An Oxalyl-CoA Synthetase Is Involved in Oxalate Degradation and Aluminum Tolerance.
abstract: Acyl Activating Enzyme3 (AAE3) was identified to be involved in the catabolism of oxalate, which is critical for seed development and defense against fungal pathogens. However, the role of AAE3 protein in abiotic stress responses is unknown. Here, we investigated the role of rice bean (Vigna umbellata) VuAAE3 in Al tolerance. Recombinant VuAAE3 protein has specific activity against oxalate, with Km= 121 ± 8.2 µm and Vmaxof 7.7 ± 0.88 µmol min-1mg-1protein, indicating it functions as an oxalyl-CoA synthetase. VuAAE3-GFP localization suggested that this enzyme is a soluble protein with no specific subcellular localization. Quantitative reverse transcription-PCR and VuAAE3 promoter-GUS reporter analysis showed that the expression induction of VuAAE3 is mainly confined to rice bean root tips. Accumulation of oxalate was induced rapidly by Al stress in rice bean root tips, and exogenous application of oxalate resulted in the inhibition of root elongation and VuAAE3 expression induction, suggesting that oxalate accumulation is involved in Al-induced root growth inhibition. Furthermore, overexpression of VuAAE3 in tobacco (Nicotiana tabacum) resulted in the increase of Al tolerance, which was associated with the decrease of oxalate accumulation. In addition, NtMATE and NtALS3 expression showed no difference between transgenic lines and wild-type plants. Taken together, our results suggest that VuAAE3-dependent turnover of oxalate plays a critical role in Al tolerance mechanisms.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (H.Q.L., J.M.X., Y.L.G., J.F.J., L.Y.L., J.L.Y., S.J.Z.).|College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China (W.F.).|Institute of Life Sciences, College of Environmental and Life Sciences, Hangzhou Normal University, Hangzhou 310036, China (W.W.C.); and.|College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China (M.R.H.).
authors.forename: He Qiang
authors.identifier: 0000-0002-2454-098X
authors.initials: HQ
authors.lastname: Lou
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (H.Q.L., J.M.X., Y.L.G., J.F.J., L.Y.L., J.L.Y., S.J.Z.).|College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China (W.F.).|Institute of Life Sciences, College of Environmental and Life Sciences, Hangzhou Normal University, Hangzhou 310036, China (W.W.C.); and.|College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China (M.R.H.).
authors.forename: Wei
authors.initials: W
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (H.Q.L., J.M.X., Y.L.G., J.F.J., L.Y.L., J.L.Y., S.J.Z.).|College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China (W.F.).|Institute of Life Sciences, College of Environmental and Life Sciences, Hangzhou Normal University, Hangzhou 310036, China (W.W.C.); and.|College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China (M.R.H.).
authors.forename: Jia Meng
authors.initials: JM
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (H.Q.L., J.M.X., Y.L.G., J.F.J., L.Y.L., J.L.Y., S.J.Z.).|College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China (W.F.).|Institute of Life Sciences, College of Environmental and Life Sciences, Hangzhou Normal University, Hangzhou 310036, China (W.W.C.); and.|College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China (M.R.H.).
authors.forename: Yu Long
authors.initials: YL
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (H.Q.L., J.M.X., Y.L.G., J.F.J., L.Y.L., J.L.Y., S.J.Z.).|College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China (W.F.).|Institute of Life Sciences, College of Environmental and Life Sciences, Hangzhou Normal University, Hangzhou 310036, China (W.W.C.); and.|College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China (M.R.H.).
authors.forename: Jian Feng
authors.initials: JF
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (H.Q.L., J.M.X., Y.L.G., J.F.J., L.Y.L., J.L.Y., S.J.Z.).|College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China (W.F.).|Institute of Life Sciences, College of Environmental and Life Sciences, Hangzhou Normal University, Hangzhou 310036, China (W.W.C.); and.|College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China (M.R.H.).
authors.forename: Wei Wei
authors.initials: WW
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (H.Q.L., J.M.X., Y.L.G., J.F.J., L.Y.L., J.L.Y., S.J.Z.).|College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China (W.F.).|Institute of Life Sciences, College of Environmental and Life Sciences, Hangzhou Normal University, Hangzhou 310036, China (W.W.C.); and.|College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China (M.R.H.).
authors.forename: Ling Yu
authors.initials: LY
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (H.Q.L., J.M.X., Y.L.G., J.F.J., L.Y.L., J.L.Y., S.J.Z.).|College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China (W.F.).|Institute of Life Sciences, College of Environmental and Life Sciences, Hangzhou Normal University, Hangzhou 310036, China (W.W.C.); and.|College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China (M.R.H.).
authors.forename: Mei Rong
authors.initials: MR
authors.lastname: Hai
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (H.Q.L., J.M.X., Y.L.G., J.F.J., L.Y.L., J.L.Y., S.J.Z.); yangjianli@zju.edu.cn.|College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China (W.F.); yangjianli@zju.edu.cn.|Institute of Life Sciences, College of Environmental and Life Sciences, Hangzhou Normal University, Hangzhou 310036, China (W.W.C.); and yangjianli@zju.edu.cn.|College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China (M.R.H.) yangjianli@zju.edu.cn.
authors.forename: Jian Li
authors.identifier: 0000-0003-0385-5787
authors.initials: JL
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China (H.Q.L., J.M.X., Y.L.G., J.F.J., L.Y.L., J.L.Y., S.J.Z.).|College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China (W.F.).|Institute of Life Sciences, College of Environmental and Life Sciences, Hangzhou Normal University, Hangzhou 310036, China (W.W.C.); and.|College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China (M.R.H.).
authors.forename: Shao Jian
authors.identifier: 0000-0002-3336-8165
authors.initials: SJ
authors.lastname: Zheng
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D000535:Aluminum / Q000633:toxicity*; D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000737:chemistry / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D009928:Organ Specificity / Q000235:genetics; D010070:Oxalates / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects / Q000502:physiology; D000070658:Vigna / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D010070:Oxalates; D010940:Plant Proteins; D000535:Aluminum; D003066:Coenzyme A Ligases