title: First Molecular Identification of Dirofilaria repens in a Dog Blood Sample from Guanajuato, Mexico.
abstract: Dirofilaria repens is the causative zoonotic agent of subcutaneous dirofilariosis. Although the American continent is considered currently free of D. repens infections, in the past few years there have been some reports suggesting the presence of this agent in the Americas. In Guanajuato, Mexico, there is a report of the presence of D. repens. To determine whether this parasite is really present in Guanajuato, a total of 177 EDTA blood samples from dogs were tested using the modified Knott's technique. Using this technique, we found 15 positive samples, which were then analyzed using the PCR technique, with a panfilarial set of primers and a specific primer pair for D. repens. In one sample, the expected band was present and the corresponding amplicon, sequenced, displayed a 100% identity to D. repens, confirming the presence of this exotic and zoonotic filarial species in the municipality of Silao, Guanajuato, Mexico.
authors.affiliation: 1 División Ciencias de la Vida, Departamento de Agronomía, P.E. de Medicina Veterinaria y Zootecnia, Universidad de Guanajuato Campus Irapuato-Salamanca , Guanajuato, México .
authors.forename: Sandra
authors.initials: S
authors.lastname: Ramos-Lopez
authors.affiliation: 2 Departamento de Alimentos, Universidad de Guanajuato Campus Irapuato-Salamanca , Guanajuato, México .|3 Posgrado en Biociencias, Universidad de Guanajuato Campus Irapuato-Salamanca , Guanajuato, México .
authors.forename: Ma Fabiola
authors.initials: MF
authors.lastname: León-Galván
authors.affiliation: 1 División Ciencias de la Vida, Departamento de Agronomía, P.E. de Medicina Veterinaria y Zootecnia, Universidad de Guanajuato Campus Irapuato-Salamanca , Guanajuato, México .
authors.forename: Mariana
authors.initials: M
authors.lastname: Salas-Alatorre
authors.affiliation: 1 División Ciencias de la Vida, Departamento de Agronomía, P.E. de Medicina Veterinaria y Zootecnia, Universidad de Guanajuato Campus Irapuato-Salamanca , Guanajuato, México .|3 Posgrado en Biociencias, Universidad de Guanajuato Campus Irapuato-Salamanca , Guanajuato, México .
authors.forename: Alma A
authors.initials: AA
authors.lastname: Lechuga-Arana
authors.affiliation: 1 División Ciencias de la Vida, Departamento de Agronomía, P.E. de Medicina Veterinaria y Zootecnia, Universidad de Guanajuato Campus Irapuato-Salamanca , Guanajuato, México .|3 Posgrado en Biociencias, Universidad de Guanajuato Campus Irapuato-Salamanca , Guanajuato, México .
authors.forename: Mauricio
authors.initials: M
authors.lastname: Valencia-Posadas
authors.affiliation: 1 División Ciencias de la Vida, Departamento de Agronomía, P.E. de Medicina Veterinaria y Zootecnia, Universidad de Guanajuato Campus Irapuato-Salamanca , Guanajuato, México .|3 Posgrado en Biociencias, Universidad de Guanajuato Campus Irapuato-Salamanca , Guanajuato, México .
authors.forename: Abner J
authors.initials: AJ
authors.lastname: Gutiérrez-Chávez
mesh_terms: D000818:Animals; D060348:Dirofilaria repens / Q000302:isolation & purification*; D004184:Dirofilariasis / Q000097:blood* / Q000453:epidemiology / Q000469:parasitology; D004283:Dog Diseases / Q000097:blood* / Q000453:epidemiology / Q000469:parasitology; D004285:Dogs; D016133:Polymerase Chain Reaction; D015047:Zoonoses
keywords: Dirofilaria repens; PCR; dog; microfilaremia; zoonosis