title: Effect of high-doses pyrogallol on oxidative damage, transcriptional responses and microcystins synthesis in Microcystis aeruginosa TY001 (Cyanobacteria).
abstract: Severe eutrophication and harmful cyanobacterial blooms of freshwater ecosystems is a persistent environmental topic in recent decades. Pyrogallol (polyphenol) was confirmed to exhibit one of the most intensive inhibitory effects on the Microcystis aeruginosa. In this study, the expression of genes, release of microcystins (MCs) and antioxidant system of pyrogallol on Microcystis aeruginosa TY001 were investigated. The results revealed that the expression of stress response genes (prx, ftsH, grpE and fabZ) and DNA repair genes (recA and gyrB) were up-regulated. Meanwhile, the antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activity, were increased, and the stress caused lipid peroxidation to occur and malondialdehyde (MDA) levels to change. Unexpectedly, the relative transcript abundance of microcystin synthesis genes (mcyB, mcyD and ntcA) and the contents of microcystins (MCs) significantly increased compared with the control in the culture medium. In conclusion, oxidative damage and DNA damage are the primary mechanisms for the allelopathic effect of pyrogallol on M. aeruginosa TY001.
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Feng
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Lv
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, China. Electronic address: xiesl@sxu.edu.cn.
authors.forename: Shu-Lian
authors.initials: SL
authors.lastname: Xie
keywords: Gene expression; Microcystins; Microcystis aeruginosa; Oxidative damage; Pyrogallol