title: 3D in vitro co-culture models based on normal cells and tumor spheroids formed by cyclic RGD-peptide induced cell self-assembly.
abstract: OBJECTIVE
  To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique.
  
  
  RESULTS
  Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. To reach reproducible architecture of co-culture spheroids, two novel 3D in vitro models with well pronounced core-shell structure from tumor spheroids and single mouse fibroblasts were developed based on this approach. The first was a combination of a RGD-peptide platform with the liquid overlay technique with further co-cultivation for 1-2 days. The second allowed co-culture spheroids to generate within polyelectrolyte microcapsules by cultivation for 2 weeks. M-3 cells (a core) and L-929 fibroblasts (a shell) were easily distinguished by confocal microscopy due to cell staining with DiO and DiI dyes, respectively.
  
  
  CONCLUSIONS
  The 3D co-culture spheroids are proposed as a tool in tumor biology to study cell-cell interactions as well as for testing novel anticancer drugs and drug delivery vehicles.
authors.affiliation: Polymers for Biology Laboratory, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, Russia, 117997. roman.akasov@gmail.com.
authors.forename: Roman
authors.identifier: http://orcid.org/0000-0001-6486-8114
authors.initials: R
authors.lastname: Akasov
authors.affiliation: Polymers for Biology Laboratory, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, Russia, 117997.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Gileva
authors.affiliation: Polymers for Biology Laboratory, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, Russia, 117997.
authors.forename: Daria
authors.initials: D
authors.lastname: Zaytseva-Zotova
authors.affiliation: Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, Saint-Petersburg, Russia, 199004.
authors.forename: Sergey
authors.initials: S
authors.lastname: Burov
authors.affiliation: UMR CNRS 7274, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la Fort de Haye, 54518, Vandoeuvre Lès Nancy, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Chevalot
authors.affiliation: UMR CNRS 7274, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la Fort de Haye, 54518, Vandoeuvre Lès Nancy, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Guedon
authors.affiliation: Polymers for Biology Laboratory, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, Russia, 117997.
authors.forename: Elena
authors.initials: E
authors.lastname: Markvicheva
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects; D018920:Coculture Techniques; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D018613:Microscopy, Confocal; D010456:Peptides, Cyclic / Q000494:pharmacology*; D018874:Spheroids, Cellular / Q000187:drug effects
chemical_list: D010456:Peptides, Cyclic; C507169:cyclic arginine-glycine-aspartic acid peptide
keywords: Fibroblasts; Liquid overlay technique; Melanoma cells; Microencapsulation; Multicellular tumor spheroids; RGD-peptide