title: A fully integrated electrochemical biosensor platform fabrication process for cytokines detection.
abstract: Interleukin-1b (IL-1b) and interleukin-10 (IL-10) biomarkers are one of many antigens that are secreted in acute stages of inflammation after left ventricle assisted device (LVAD) implantation for patients suffering from heart failure (HF). In the present study, we have developed a fully integrated electrochemical biosensor platform for cytokine detection at minute concentrations. Using eight gold working microelectrodes (WEs) the design will increase the sensitivity of detection, decrease the time of measurements, and allow a simultaneous detection of varying cytokine biomarkers. The biosensor platform was fabricated onto silicon substrates using silicon technology. Monoclonal antibodies (mAb) of anti-human IL-1b and anti-human IL-10 were electroaddressed onto the gold WEs through functionalization with 4-carboxymethyl aryl diazonium (CMA). Cyclic voltammetry (CV) was applied during the WE functionalization process to characterize the gold WE surface properties. Finally, electrochemical impedance spectroscopy (EIS) characterized the modified gold WE. The biosensor platform was highly sensitive to the corresponding cytokines and no interference with other cytokines was observed. Both cytokines: IL-10 and IL-1b were detected within the range of 1pgmL-1to 15pgmL-1. The present electrochemical biosensor platform is very promising for multi-detection of biomolecules which can dramatically decrease the time of analysis. This can provide data to clinicians and doctors concerning cytokines secretion at minute concentrations and the prediction of the first signs of inflammation after LVAD implantation.
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, Université Lyon 1, ENS Lyon -5, rue de la Doua, F-69100 Villeurbanne, France.
authors.forename: Abdoullatif
authors.initials: A
authors.lastname: Baraket
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, Université Lyon 1, ENS Lyon -5, rue de la Doua, F-69100 Villeurbanne, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Lee
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, Université Lyon 1, ENS Lyon -5, rue de la Doua, F-69100 Villeurbanne, France.
authors.forename: Nadia
authors.initials: N
authors.lastname: Zine
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, Université Lyon 1, ENS Lyon -5, rue de la Doua, F-69100 Villeurbanne, France.
authors.forename: Monique
authors.initials: M
authors.lastname: Sigaud
authors.affiliation: Centro Nacional de Microelectrónica (IMB-CSIC) Campus UAB, Bellaterra, 08193 Barcelona, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Bausells
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR 5280, CNRS, Université Lyon 1, ENS Lyon -5, rue de la Doua, F-69100 Villeurbanne, France. Electronic address: abdelhamid.errachid-el-salhi@univ-lyon1.fr.
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Errachid
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D016207:Cytokines / Q000737:chemistry / Q000302:isolation & purification; D058266:Dielectric Spectroscopy; D006046:Gold / Q000737:chemistry; D006333:Heart Failure / Q000378:metabolism / Q000473:pathology; D006352:Heart Ventricles / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D016753:Interleukin-10 / Q000737:chemistry / Q000302:isolation & purification*; D053583:Interleukin-1beta / Q000737:chemistry / Q000302:isolation & purification*; D012825:Silicon / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D016207:Cytokines; D053583:Interleukin-1beta; D016753:Interleukin-10; D006046:Gold; D012825:Silicon
keywords: Biosensor platform; Cytokines; Electrochemical impedance spectroscopy; Heart failure; Multi-detection