title: Recombinant beta interferon could clear the low-dose infected porcine reproductive and respiratory syndrome virus (PRRSV) in MARC-145 cells.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) causes one of the most economically devastating and pandemic porcine diseases. Previous study has shown that MARC-145 cells pretreated with recombinant IFN-β (rIFN-β) couldn't develop cytopathic effect (CPE) of PRRSV. However, up to date, it is not clear whether MARC-145 cells post-treated with rIFN-β could develop CPE of PRRSV. The present work showed that the MARC-145 cells didn't develop the CPE at 120 hr post-infection (p.i.) with low-dose of PRRSV when the cells were pre-treated with rIFN-β (Group 1), post-treated with rIFN-β at 4 hr p.i. (Group 2), or post-treated with rIFN-β at 8 hr p.i. (Group 3), while the MARC-145 cells could develop CPE when the cells were infected with high-dose PRRSV and then treated with rIFN-β at 24 hr p.i.. Furthermore, the indirect immunofluorescence assay confirmed that there were a few N protein-positive cells in the high-dose infected cells in Group 1, Group 2 and Group 3, while there were no N protein-positive cells in the low-dose infected cells in all rIFN-β treatment groups. In addition, the numbers of N protein-positive cells in high-dose infected cells (MOI = 10) in Group 1 were lower than that in Group 2 and Group 3. The results above demonstrated that both pre-treatment with rIFN-β and an earlier post-treatment with rIFN-β could inhibit the PRRSV replication and could clear the low-dose infected PRRSV, which indicated that the rIFN-β had efficient antiviral activities when the cells have been infected with PRRSV.
authors.forename: X
authors.initials: X
authors.lastname: Shi
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Jiang
authors.forename: R
authors.initials: R
authors.lastname: Deng
authors.forename: A
authors.initials: A
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002522:Chlorocebus aethiops; D005347:Fibroblasts / Q000187:drug effects / Q000821:virology*; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D011994:Recombinant Proteins
chemical_list: D011994:Recombinant Proteins; D016899:Interferon-beta
keywords: PRRSV; recombinant IFN-β.