title: Protective Effect of Amphipterygium adstringens Extract on Dextran Sulphate Sodium-Induced Ulcerative Colitis in Mice.
abstract: Amphipterygium adstringens is an endemic species in Mexico commonly known as "cuachalalate." Healers to treat gastritis, gastric ulcers, and gastrointestinal cancer have traditionally used the bark. We investigated the effects of alcoholic extract of A. adstringens (AaEE) in DSS-induced colitis in mice. The protective effect of AaEE was determined at 200 mg/kg by oral gavage for 10 days. We determine the effect of AaEE on clinical features (disease activity index), antioxidants, anti-inflammatory, and immunomodulatory activities in relation to the activity of SOD, CAT, and GPx, levels of proinflammatory cytokines, and changes both macroscopic and microscopic of the colonic mucosa. AaEE significantly reduced the inflammation of colon and significantly increased SOD and GPx activities. AaEE also significantly decreased TNF-α, IFN-γ, and IL-1β cytokine levels compared to DSS-treated mice and reduced both infiltration of inflammatory cells and the mucosal damage in colon. The results suggested the protective potential of AaEE in DSS-induced colitis and this might be attributed to its phytochemicals compounds that have been found to induce a wide spectrum of activities such as reduction in oxidative stress, suppression of inflammation, modulating numerous signal transduction pathways, and induction of apoptosis. The findings of this study suggest that AaEE has substantial potential for the treatment of inflammatory colitis.
authors.affiliation: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, 11400 Ciudad de México, Mexico.
authors.forename: Mario
authors.initials: M
authors.lastname: Rodriguez-Canales
authors.affiliation: Laboratorio de Inmunobiología, Carrera de Medicina, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors.forename: Ruben
authors.initials: R
authors.lastname: Jimenez-Rivas
authors.affiliation: Laboratorio de Farmacognosia, UBIPRO, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors.forename: Maria Margarita
authors.initials: MM
authors.lastname: Canales-Martinez
authors.affiliation: Laboratorio de Inmunobiología, Carrera de Medicina, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors.forename: Ana Judith
authors.initials: AJ
authors.lastname: Garcia-Lopez
authors.affiliation: Laboratorio de Inmunobiología, Carrera de Medicina, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors.forename: Nelly
authors.initials: N
authors.lastname: Rivera-Yañez
authors.affiliation: Laboratorio de Inmunobiología, Carrera de Medicina, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors.forename: Oscar
authors.initials: O
authors.lastname: Nieto-Yañez
authors.affiliation: Unidad de Biomedicina, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors.forename: Yadira
authors.initials: Y
authors.lastname: Ledesma-Soto
authors.affiliation: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, 11400 Ciudad de México, Mexico.
authors.forename: Luvia Enid
authors.initials: LE
authors.lastname: Sanchez-Torres
authors.affiliation: Unidad de Biomedicina, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors.forename: Miriam
authors.initials: M
authors.lastname: Rodriguez-Sosa
authors.affiliation: Unidad de Biomedicina, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors.forename: Luis Ignacio
authors.initials: LI
authors.lastname: Terrazas
authors.affiliation: Laboratorio de Inmunobiología, Carrera de Medicina, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico.
authors.forename: Marco Aurelio
authors.identifier: 0000-0002-6780-0710
authors.initials: MA
authors.lastname: Rodriguez-Monroy
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis; D002374:Catalase / Q000378:metabolism; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy*; D003106:Colon / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology*; D029631:Sapindaceae / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016207:Cytokines; D010936:Plant Extracts; D016264:Dextran Sulfate; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase