title: Epithelia Use Butyrophilin-like Molecules to Shape Organ-Specific γδ T Cell Compartments.
abstract: Many body surfaces harbor organ-specific γδ T cell compartments that contribute to tissue integrity. Thus, murine dendritic epidermal T cells (DETCs) uniquely expressing T cell receptor (TCR)-Vγ5 chains protect from cutaneous carcinogens. The DETC repertoire is shaped by Skint1, a butyrophilin-like (Btnl) gene expressed specifically by thymic epithelial cells and suprabasal keratinocytes. However, the generality of this mechanism has remained opaque, since neither Skint1 nor DETCs are evolutionarily conserved. Here, Btnl1 expressed by murine enterocytes is shown to shape the local TCR-Vγ7(+) γδ compartment. Uninfluenced by microbial or food antigens, this activity evokes the developmental selection of TCRαβ(+) repertoires. Indeed, Btnl1 and Btnl6 jointly induce TCR-dependent responses specifically in intestinal Vγ7(+) cells. Likewise, human gut epithelial cells express BTNL3 and BTNL8 that jointly induce selective TCR-dependent responses of human colonic Vγ4(+) cells. Hence, a conserved mechanism emerges whereby epithelia use organ-specific BTNL/Btnl genes to shape local T cell compartments.
authors.affiliation: Francis Crick Institute, London WC2A3LY, UK; Peter Gorer Department of Immunobiology, King's College London, London SE19RT, UK; MBPhD Programme, University College London, London WC1E 6BT, UK.
authors.forename: Rafael
authors.initials: R
authors.lastname: Di Marco Barros
authors.affiliation: Francis Crick Institute, London WC2A3LY, UK.
authors.forename: Natalie A
authors.initials: NA
authors.lastname: Roberts
authors.affiliation: Francis Crick Institute, London WC2A3LY, UK; Peter Gorer Department of Immunobiology, King's College London, London SE19RT, UK; Department of Gastroenterology, Guy's and St Thomas' Foundation Trust, London SE17EH, UK.
authors.forename: Robin J
authors.initials: RJ
authors.lastname: Dart
authors.affiliation: Peter Gorer Department of Immunobiology, King's College London, London SE19RT, UK.
authors.forename: Pierre
authors.initials: P
authors.lastname: Vantourout
authors.affiliation: Francis Crick Institute, London WC2A3LY, UK.
authors.forename: Anett
authors.initials: A
authors.lastname: Jandke
authors.affiliation: Peter Gorer Department of Immunobiology, King's College London, London SE19RT, UK.
authors.forename: Oliver
authors.initials: O
authors.lastname: Nussbaumer
authors.affiliation: Francis Crick Institute, London WC2A3LY, UK.
authors.forename: Livija
authors.initials: L
authors.lastname: Deban
authors.affiliation: Francis Crick Institute, London WC2A3LY, UK.
authors.forename: Sara
authors.initials: S
authors.lastname: Cipolat
authors.affiliation: Francis Crick Institute, London WC2A3LY, UK.
authors.forename: Rosie
authors.initials: R
authors.lastname: Hart
authors.affiliation: Peter Gorer Department of Immunobiology, King's College London, London SE19RT, UK.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Iannitto
authors.affiliation: Peter Gorer Department of Immunobiology, King's College London, London SE19RT, UK.
authors.forename: Adam
authors.initials: A
authors.lastname: Laing
authors.affiliation: Francis Crick Institute, London WC2A3LY, UK.
authors.forename: Bradley
authors.initials: B
authors.lastname: Spencer-Dene
authors.affiliation: Francis Crick Institute, London WC2A3LY, UK.
authors.forename: Philip
authors.initials: P
authors.lastname: East
authors.affiliation: Peter Gorer Department of Immunobiology, King's College London, London SE19RT, UK.
authors.forename: Deena
authors.initials: D
authors.lastname: Gibbons
authors.affiliation: Department of Gastroenterology, Guy's and St Thomas' Foundation Trust, London SE17EH, UK.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Irving
authors.affiliation: Department of Immunology, Pasteur Institute, 75015 Paris, France.
authors.forename: Pablo
authors.initials: P
authors.lastname: Pereira
authors.affiliation: Institute for Medical Microbiology and Hospital Epidemiology, University of Marburg, 35037 Marburg, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Steinhoff
authors.affiliation: Francis Crick Institute, London WC2A3LY, UK; Peter Gorer Department of Immunobiology, King's College London, London SE19RT, UK. Electronic address: adrian.hayday@kcl.ac.uk.
authors.forename: Adrian
authors.initials: A
authors.lastname: Hayday
mesh_terms: D000818:Animals; D000071797:Butyrophilins / Q000235:genetics / Q000276:immunology*; D055786:Gene Knockout Techniques; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
chemical_list: C584968:BTNL8 protein, human; C558164:Btnl1 protein, mouse; D000071797:Butyrophilins; D016692:Receptors, Antigen, T-Cell, gamma-delta