title: Promigratory and proangiogenic effects of AdipoRon on bone marrow-derived mesenchymal stem cells: an in vitro study.
abstract: OBJECTIVE
  To investigate the effect of AdipoRon on major factors involved in survival, migration and neovascularization of rat bone marrow-derived mesenchymal stem cells.
  
  
  RESULTS
  AdipoRon promoted the MSCs viability. Real-time PCR indicated that the expression of cyclooxygenase-2 (COX-2), hypoxia-inducible factor-1 (HIF-1) C-X-C chemokine receptor type 4 (CXCR4), C-C chemokine receptor type 2 (CCR2), vascular endothelial growth factor matrix metalloproteinase-2 (MMP-2) and MMP-9 were upregulated in AdipoRon-treated MSCs compared to control groups. Prostaglandin E2 (PGE2) level, as well as migration ability of MSCs (scratch assay) was enhanced by AdipoRon preconditioning.
  
  
  CONCLUSIONS
  Preconditioning of MSCs with AdipoRon prior to transplantation could enhance cell survival, angiogenesis and migration via activating the COX-2/PGE2/HIF-1 pathway and other contributing factors.
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Malih
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Massoud
authors.initials: M
authors.lastname: Saidijam
authors.affiliation: Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Kamran
authors.initials: K
authors.lastname: Mansouri
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Pourjafar
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Maryam Sadat
authors.initials: MS
authors.lastname: Tafakh
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Talebzadeh
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. re.najafi@umsha.ac.ir.|Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. re.najafi@umsha.ac.ir.
authors.forename: Rezvan
authors.initials: R
authors.lastname: Najafi
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D010880:Piperidines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D054390:Receptors, CCR2 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism
chemical_list: C585113:AdipoRon; C515336:Ccr2 protein, rat; C486264:Cxcr4 protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D010880:Piperidines; D054390:Receptors, CCR2; D019718:Receptors, CXCR4; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: AdipoRon; Adiponectin; Cyclooxygenase-2; HIF-1; Mesenchymal stem cell; Prostaglandin E2