title: Cobalt-60 gamma-ray irradiation pretreatment and sludge protein for enhancing enzymatic saccharification of hybrid poplar sawdust.
abstract: In order to improve the enzymatic saccharification of hybrid poplar sawdust, gamma irradiation pretreatment and enzymatic hydrolysis in the presence of sludge protein were investigated. The cellulose crystallinity index were significantly decreased after irradiation pretreatment, and adding sludge protein improved enzyme activity and increased the reducing sugar yield. The conditions of irradiation pretreatment and enzymatic hydrolysis in the presence of sludge protein were systematically examined. The maximum reducing sugar yield was 519mg/g under an irradiation dose of 300kGy, a sludge protein dosage of 2mg/mL, an enzymatic hydrolysis temperature of 45°C, an enzymatic hydrolysis time of 84h, and a 90FPU/g enzyme loading. This work indicated that the combined method of gamma irradiation pretreatment and enzymatic hydrolysis in the presence of sludge protein was a promising potential for the saccharification of hybrid poplar sawdust.
authors.affiliation: College of Chemistry and Chemical Engineering, Yulin University, Yulin 719000, Shaanxi Province, China. Electronic address: yulinx@126.com.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Department of Management Engineering, Qiqihar Institute of Engineering, Qiqihar 161005, Heilongjiang Province, China.
authors.forename: Yuxiu
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: College of Chemistry and Chemical Engineering, Yulin University, Yulin 719000, Shaanxi Province, China.
authors.forename: Lipeng
authors.initials: L
authors.lastname: Wang
mesh_terms: D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D003037:Cobalt Radioisotopes; D006868:Hydrolysis; D012722:Sewage / Q000737:chemistry*; D014934:Wood / Q000737:chemistry / Q000528:radiation effects
chemical_list: D003037:Cobalt Radioisotopes; D012722:Sewage; D002482:Cellulose; D002480:Cellulase
keywords: Enzymatic hydrolysis; Gamma irradiation pretreatment; Hybrid poplar sawdust; Sludge protein