title: Stabilization and detection of hydrophylloquinone as di-O-methyl derivative.
abstract: Phylloquinone is a redox active naphthoquinone involved in electron transport in plants. The function of this reduced form remains unclear due to its instability, which has precluded detection. Herein, a simple method that permits the stabilization of the reduced form of phylloquinone by di-O-methylation and HPLC detection is described.
authors.affiliation: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, SP 05508-000, Brazil.
authors.forename: Rodrigo A C
authors.initials: RAC
authors.lastname: Sussmann
authors.affiliation: Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, São Paulo, SP, 05508-000, Brazil.
authors.forename: Marcilio M
authors.initials: MM
authors.lastname: de Moraes
authors.affiliation: Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, São Paulo, SP, 05508-000, Brazil; Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Cebrián-Torrejón
authors.affiliation: Instituto de Química Rosario (IQUIR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina.
authors.forename: Exequiel O
authors.initials: EO
authors.lastname: Porta
authors.affiliation: Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Doménech-Carbó
authors.affiliation: Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, São Paulo, SP, 05508-000, Brazil.
authors.forename: Lydia F
authors.initials: LF
authors.lastname: Yamaguchi
authors.affiliation: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, SP 05508-000, Brazil.
authors.forename: Alejandro M
authors.initials: AM
authors.lastname: Katzin
authors.affiliation: Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, São Paulo, SP, 05508-000, Brazil. Electronic address: majokato@iq.usp.br.
authors.forename: Massuo J
authors.initials: MJ
authors.lastname: Kato
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D008745:Methylation; D010837:Vitamin K 1 / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry*
chemical_list: C121368:dihydrophylloquinone; D010837:Vitamin K 1
keywords: Di-O-methyl derivative; Electrochemistry; HPLC; Hydrophylloquinone; Phylloquinone; Redox