title: Overexpression of Long Non-Coding RNA TUG1 Promotes Colon Cancer Progression.
abstract: BACKGROUND Colon cancer is one of the most prevalent and deadly cancers worldwide. It is still necessary to further define the mechanisms and explore therapeutic targets of colon cancer. Dysregulation of long noncoding RNAs (lncRNAs) has been shown to be correlated with diverse biological processes, including tumorigenesis. This study aimed to characterize the biological mechanism of taurine-upregulated gene 1 (TUG1) in colon cancer. MATERIAL AND METHODS qRT-PCR was used to analyze the expression level of TUG1 and p63 in 75 colon cancer tissues and the matched adjacent non-tumor tissue. In vitro, cultured colon cancer cell lines HCT-116 and LoVo were used as cell models. TUG1 and p63 were silenced via transferring siRNA into HCT-116 or LoVo. The effects of TUG1 were investigated by examining cell proliferation, apoptosis, and migration. RESULTS Among the 75 colon cancer cases, the expression of TUG1 was significantly higher in colon cancer tissues compared with the matched adjacent non-tumor tissue, while p63 expression was lower in the tumor tissue. In HCT-116 and LoVo, the expression of TUG1 was significantly increased by p63 siRNA transfection. Furthermore, down-regulation of TUG1 by siRNA significantly inhibited the cell proliferation and promoted colon cancer cell apoptosis. In addition, inhibition of TUG1 expression significantly blocked the cell migration ability of colon cancer cells. CONCLUSIONS LncRNA TUG1 may serve as a potential oncogene for colon cancer. Overexpressed TUG1 may contribute to promoting cell proliferation and migration in colon cancer cells.
authors.affiliation: Department of Gastrointestinal Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong, China (mainland).
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Zhai
authors.affiliation: Department of Oncology, Yantai Yuhuangding Hospital, Yantai, Shandong, China (mainland).
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Sui
authors.affiliation: Department ofEndocrinology, Yantai Yuhuangding Hospital, Yantai, Shandong, China (mainland).
authors.forename: Xiao
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Oncology, Yantai Yuhuangding Hospital, Yantai, Shandong, China (mainland).
authors.forename: Zhen
authors.initials: Z
authors.lastname: Qu
authors.affiliation: Department of Gastrointestinal Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong, China (mainland).
authors.forename: Jin-Chen
authors.initials: JC
authors.lastname: Hu
authors.affiliation: Department of Gastrointestinal Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong, China (mainland).
authors.forename: Hai-Qing
authors.initials: HQ
authors.lastname: Sun
authors.affiliation: Department of Gastrointestinal Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong, China (mainland).
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Zheng
authors.affiliation: Inpatient Area Of Genenral Surgery Department II, The First People`s Hospital Of Taian, Taian, Shandong, China (mainland).
authors.forename: Kai
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Department of Gastrointestinal Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong, China (mainland).
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Jiang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D013654:Taurine / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C081876:CKAP4 protein, human; D008565:Membrane Proteins; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; C000598536:TUG1 long noncoding RNA, human; D013654:Taurine