title: First deep screening of bacterial assemblages associated with corals of the Tropical Eastern Pacific.
abstract: Bacterial assemblages associated with the hermatypic corals Pocillopora damicornis and P. verrucosa, the surrounding seawater and the sediment at six coral reef sites in the north section of the Tropical Eastern Pacific were assessed using MiSeq Illumina sequencing of the V4 region of the 16S rDNA. The bacterial microbiota in both coral species, seawater and sediment were stable to seasonal variations. Bacterial assemblages between the same substrates were not significantly different from each other in the six sites sampled. Interestingly, the bacterial composition between substrates within the same site was significantly different, or not, depending on the conservation status of the site. Moreover, we found species-specific bacterial OTUs in both coral species. Analyzing the relationship between bacterial composition and environmental variables revealed a positive correlation between bacterial assemblages and dissolved oxygen, ammonium and silicate.
authors.affiliation: Programa de Doctorado en Ciencias en Biosistemática, Ecología y Manejo de Recursos Naturales y Agrícolas (BEMARENA), Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Camino Ramón Padilla Sánchez No. 2100, Nextipac, Zapopan, Jalisco, CP 45110, México Laboratorio de Ecosistemas Marinos y Acuicultura, Departamento de Ecología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Camino Ramón Padilla Sánchez No. 2100, Nextipac, Zapopan, Jalisco, CP 45110, México.
authors.forename: Joicye
authors.initials: J
authors.lastname: Hernández-Zulueta
authors.affiliation: Instituto de Ciencias Naturales 'Alexander Von Humboldt', Instituto Antofagasta, Universidad de Antofagasta, PO Box 170, Antofagasta, Chile.
authors.forename: Rubén
authors.initials: R
authors.lastname: Araya
authors.affiliation: Departamento de Botánica y Zoología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Camino Ramón Padilla Sánchez No. 2100, Nextipac, Zapopan, Jalisco, CP 45110, México.
authors.forename: Ofelia
authors.initials: O
authors.lastname: Vargas-Ponce
authors.affiliation: Laboratorio de Ecosistemas Marinos y Acuicultura, Departamento de Ecología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Camino Ramón Padilla Sánchez No. 2100, Nextipac, Zapopan, Jalisco, CP 45110, México.
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Díaz-Pérez
authors.affiliation: Laboratorio de Ecología Marina, Centro Universitario de la Costa, Universidad de Guadalajara, Av. Universidad 203, Del. Ixtapa, Puerto Vallarta, Jalisco, CP 48280, México.
authors.forename: Alma P
authors.initials: AP
authors.lastname: Rodríguez-Troncoso
authors.affiliation: Instituto de Ciencias Naturales 'Alexander Von Humboldt', Instituto Antofagasta, Universidad de Antofagasta, PO Box 170, Antofagasta, Chile.
authors.forename: Janja
authors.initials: J
authors.lastname: Ceh
authors.affiliation: Laboratorio de Ecosistemas Marinos y Acuicultura, Departamento de Ecología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Camino Ramón Padilla Sánchez No. 2100, Nextipac, Zapopan, Jalisco, CP 45110, México.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Ríos-Jara
authors.affiliation: Laboratorio de Ecosistemas Marinos y Acuicultura, Departamento de Ecología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Camino Ramón Padilla Sánchez No. 2100, Nextipac, Zapopan, Jalisco, CP 45110, México rzf39259@cucba.udg.mx.
authors.forename: Fabián A
authors.initials: FA
authors.lastname: Rodríguez-Zaragoza
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D057894:Coral Reefs; D004269:DNA, Bacterial / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D008800:Mexico; D064307:Microbiota / Q000235:genetics*; D010100:Oxygen / Q000378:metabolism; D010140:Pacific Ocean; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D017640:Silicates / Q000378:metabolism; D013045:Species Specificity
chemical_list: D064751:Ammonium Compounds; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D017640:Silicates; D010100:Oxygen
keywords: 16S rDNA; Tropical Eastern Pacific; bacterial assemblages; corals; high-throughput sequencing; microbial ecology