title: Body Fat Mass Is Associated With Ratio of Steroid Metabolites Reflecting 17,20-Lyase Activity in Prepubertal Girls.
abstract: Pediatric obesity has been related to hyperandrogenism and premature adrenarche in previous studies. However, little is known regarding the association between body fat mass and steroidogenic enzyme activities in children.
  
  
  
  To examine whether body fat mass is associated with serum steroid profiles in girls.
  
  
  
  We enrolled 242 girls (125 prepubertal, 117 pubertal; age, 7-13 years). Early morning blood samples were drawn at a university hospital to measure serum steroid profiles using gas chromatography-mass spectrometry, and steroidogenic enzyme activities were assessed from the ratios of steroid metabolites.
  
  
  
  We evaluated serum steroid profiles and estimated steroidogenic enzyme activities and their association with anthropometric indices and body composition.
  
  
  
  Prepubertal obese girls demonstrated significantly higher progestin, androgens (dehydroepiandrosterone [DHEA], androstenedione [A-dione], T, androsterone), and ratio of steroid metabolites reflecting 17,20-lyase activity [(DHEA + A-dione)/17-hydroxypregnenolone] compared with prepubertal controls. Pubertal obese girls demonstrated significantly higher serum T and androsterone than pubertal controls; however, serum steroid metabolite ratios reflecting steroidogenic enzyme activities did not significantly differ among obese and non-obese girls. Partial correlation analysis revealed that body fat mass was positively correlated with pregnenolone, DHEA, A-dione, T, androsterone, and ratio of (DHEA + A-dione)/17-hydroxypregnenolone in prepubertal girls only. Prepubertal girls with increased body fat mass had significantly higher serum DHEA and ratio of (DHEA + A-dione)/17-hydroxypregnenolone than controls.
  
  
  
  Increased androgen production in prepubertal obese girls could be at least partly due to increased body fat mass and 17,20-lyase activity.
authors.affiliation: Department of Pediatrics (S.-H.K., M.-J.P.), Sanggye Paik Hospital, Inje University College of Medicine, Seoul 01757, Korea; Molecular Recognition Research Center (J.-Y.M., M.H.C.), Korea Institute of Science and Technology, Seoul 02792, Korea; and Department of Pathology (H.S.), Tohoku University School of Medicine, Sendai 980-8575, Japan.
authors.forename: Shin-Hye
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Pediatrics (S.-H.K., M.-J.P.), Sanggye Paik Hospital, Inje University College of Medicine, Seoul 01757, Korea; Molecular Recognition Research Center (J.-Y.M., M.H.C.), Korea Institute of Science and Technology, Seoul 02792, Korea; and Department of Pathology (H.S.), Tohoku University School of Medicine, Sendai 980-8575, Japan.
authors.forename: Ju-Yeon
authors.initials: JY
authors.lastname: Moon
authors.affiliation: Department of Pediatrics (S.-H.K., M.-J.P.), Sanggye Paik Hospital, Inje University College of Medicine, Seoul 01757, Korea; Molecular Recognition Research Center (J.-Y.M., M.H.C.), Korea Institute of Science and Technology, Seoul 02792, Korea; and Department of Pathology (H.S.), Tohoku University School of Medicine, Sendai 980-8575, Japan.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Sasano
authors.affiliation: Department of Pediatrics (S.-H.K., M.-J.P.), Sanggye Paik Hospital, Inje University College of Medicine, Seoul 01757, Korea; Molecular Recognition Research Center (J.-Y.M., M.H.C.), Korea Institute of Science and Technology, Seoul 02792, Korea; and Department of Pathology (H.S.), Tohoku University School of Medicine, Sendai 980-8575, Japan.
authors.forename: Man Ho
authors.initials: MH
authors.lastname: Choi
authors.affiliation: Department of Pediatrics (S.-H.K., M.-J.P.), Sanggye Paik Hospital, Inje University College of Medicine, Seoul 01757, Korea; Molecular Recognition Research Center (J.-Y.M., M.H.C.), Korea Institute of Science and Technology, Seoul 02792, Korea; and Department of Pathology (H.S.), Tohoku University School of Medicine, Sendai 980-8575, Japan.
authors.forename: Mi-Jung
authors.initials: MJ
authors.lastname: Park
mesh_terms: D006907:17-alpha-Hydroxypregnenolone / Q000097:blood*; D000273:Adipose Tissue; D000293:Adolescent; D000728:Androgens / Q000097:blood*; D002648:Child; D005260:Female; D006801:Humans; D050177:Overweight / Q000097:blood*; D063766:Pediatric Obesity / Q000097:blood*; D011372:Progestins / Q000097:blood*; D011627:Puberty / Q000097:blood*; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism*
chemical_list: D000728:Androgens; D011372:Progestins; D006907:17-alpha-Hydroxypregnenolone; D013254:Steroid 17-alpha-Hydroxylase