title: The association of circulating microRNA-30c with atherogenic lipoprotein subfractions and composition.
abstract: Circulating miR-30c has been linked to various aspects of cholesterol homeostasis. The aim of this study was to determine the association of circulating miR-30c with the atherogenic lipoprotein subfractions. Samples from subjects who were given placebo (n=22) in a randomised, double-blind crossover study were used. Subjects were divided into non-atherogenic lipoprotein phenotype (Non-ALP; n=12; triglycerides <2.0mmol/L) and atherogenic lipoprotein phenotype (ALP; n=10; triglycerides ≥2.0mmol/L) groups. All lipid and lipoprotein measurements, RNA extraction and reverse transcription-quantitative real-time polymerase chain reaction were undertaken using standard procedures. Subjects with ALP weighed significantly more than their non-ALP counterparts (p=0.023). In the non-ALP group there was significant correlation between miR-30c and components within VLDL1, namely triglyceride which showed a negative association (p=0.035) whereas phospholipids and cholesterol-ester were both positively correlated (p=0.025 and 0.014, respectively). In contrast, in the ALP group there was a significant correlation between the expression of miR-30c and components within VLDL2, namely triglyceride, which was positively associated (p=0.013). This study reveals specificity with regards to the effect of miR-30c on VLDL subfractions based on the individual's lipoprotein phenotype and implicates roles for microsomal-triglyceride transfer-protein and cholesteryl-ester-transfer-protein in LDL and VLDL metabolism, respectively.
authors.affiliation: Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, United Kingdom.
authors.forename: Jarlath
authors.initials: J
authors.lastname: Eastwood
authors.affiliation: Department of Biochemistry, Royal Lancaster Infirmary, University Hospitals of Morecambe Bay NHS Foundation Trust, Lancaster, Lancashire, LA1 4RP, United Kingdom.
authors.forename: Muriel J
authors.initials: MJ
authors.lastname: Caslake
authors.affiliation: Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, United Kingdom; Department of Biochemistry, Royal Lancaster Infirmary, University Hospitals of Morecambe Bay NHS Foundation Trust, Lancaster, Lancashire, LA1 4RP, United Kingdom. Electronic address: ravsodi@yahoo.com.
authors.forename: Ravinder
authors.initials: R
authors.lastname: Sodi
mesh_terms: D050197:Atherosclerosis / Q000097:blood*; D018592:Cross-Over Studies; D004311:Double-Blind Method; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D035683:MicroRNAs / Q000097:blood*; D010641:Phenotype
chemical_list: D008074:Lipoproteins; C546086:MIRN30b microRNA, human; D035683:MicroRNAs
keywords: Atherogenic-lipoproteins; Cholesteryl-ester-transfer-protein (CETP); Circulating miR-30c; Microsomal-triglyceride transfer-protein (MTP)