title: Plasma-Treated Microplates with Enhanced Protein Recoveries and Minimized Extractables.
abstract: SiO2Medical Products, Inc. (SiO) has developed a proprietary technology that greatly enhances protein recoveries and reduces extractables from commercial microplates used for bioanalytical assays and storage of biologics. SiO technology is based on plasma treatment that chemically modifies the surface of polypropylene with predominantly hydrogen-bond-acceptor uncharged polar groups. The resultant surface resists nonspecific protein adsorption over a wide range of protein concentrations, thereby eliminating the need to passivate (and hence potentially contaminate) the microplates with blocking proteins. High shelf-life stability and cleanliness of the plasma-treated microplates have been demonstrated using five different proteins for two common microplate formats. The protein recovery performance of plasma-treated microplates is found to be higher compared with commercial low-protein-binding microplates.
authors.affiliation: 1 SiO2 Medical Products, Inc., Auburn, AL, USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Weikart
authors.affiliation: 2 Departments of Chemistry and Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Klibanov
authors.affiliation: 1 SiO2 Medical Products, Inc., Auburn, AL, USA.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Breeland
authors.affiliation: 1 SiO2 Medical Products, Inc., Auburn, AL, USA.
authors.forename: Ahmad H
authors.initials: AH
authors.lastname: Taha
authors.affiliation: 1 SiO2 Medical Products, Inc., Auburn, AL, USA.
authors.forename: Brian R
authors.initials: BR
authors.lastname: Maurer
authors.affiliation: 1 SiO2 Medical Products, Inc., Auburn, AL, USA.
authors.forename: Steven P
authors.initials: SP
authors.lastname: Martin
mesh_terms: D000818:Animals; D020099:Coated Materials, Biocompatible; D004864:Equipment and Supplies; D006801:Humans; D013677:Medical Laboratory Science / Q000295:instrumentation* / Q000379:methods; D010949:Plasma; D011126:Polypropylenes; D011506:Proteins / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D011126:Polypropylenes; D011506:Proteins
keywords: extractables and leachables; microplates; nonspecific; polypropylene plasma treatment; protein adsorption; protein recovery