title: Circulating tumor DNA detection in lung cancer patients before and after surgery.
abstract: Circulating tumor DNA (ctDNA) in peripheral blood is a "liquid biopsy" that contains representative tumor information including gene mutations. Additionally, repeated ctDNA samples can be easily obtained to monitor response to treatment and disease progression, which may be especially valuable to lung cancer patients with tumors that cannot be easily biopsied or removed. To investigate the changes in ctDNA after surgical tumor resection, tumor and blood samples obtained before and after surgery were collected prospectively from 41 non-small lung cancer (NSCLC) patients. Somatic driver mutations in tumor DNA (tDNA) and pre- and post-op plasma ctDNA sample pairs were identified by targeted sequencing in several genes including EGFR, KRAS, and TP53 with an overall study concordance of 78.1% and sensitivity and specificity of 69.2% and 93.3%, respectively. Importantly, the frequency of 91.7% of ctDNA mutations decreased after surgery and these changes were observed as little as 2 days post-op. Moreover, the presence of ctDNA had a higher positive predictive value than that of six tumor biomarkers in current clinical use. This study demonstrates the use of targeted sequencing to reliably identify ctDNA changes in response to treatment, indicating a potential utility of this approach in the clinical management of NSCLC.
authors.affiliation: Medical School of Chinese PLA, Beijing, China.|Department of Cardiothoracic Surgery, the First Affiliated Hospital of General Hospital of the Chinese People's Liberation Army, Beijing, China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Guo
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lou
authors.affiliation: Department of Thoracic Surgery, The General Hospital of People's Liberation Army, Beijing, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Thoracic Surgery, The General Hospital of People's Liberation Army, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, The General Hospital of People's Liberation Army, Beijing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Ye
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Zhang
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Zhao
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Wu
authors.affiliation: San Valley Biotechnology Incorporated, Beijing, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Shi
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Lindsey
authors.initials: L
authors.lastname: Jones
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Katherine S
authors.initials: KS
authors.lastname: Chen
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Xue F
authors.initials: XF
authors.lastname: Huang
authors.affiliation: Norris Comprehensive Cancer Center, Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Si-Yi
authors.initials: SY
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, The General Hospital of People's Liberation Army, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000601:surgery; D000074141:Circulating Tumor DNA / Q000097:blood*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D059645:Mutation Rate
chemical_list: D014408:Biomarkers, Tumor; D000074141:Circulating Tumor DNA