title: Characterization and evaluation of Bacillus amyloliquefaciens strain WF02 regarding its biocontrol activities and genetic responses against bacterial wilt in two different resistant tomato cultivars.
abstract: Bacillus amyloliquefaciens strain WF02, isolated from soil collected at Wufeng Mountain, Taiwan, has siderophore-producing ability and in vitro antagonistic activity against bacterial wilt pathogen. To determine the impact of plant genotype on biocontrol effectiveness, we treated soil with this strain before infecting susceptible (L390) and moderately resistant (Micro-Tom) tomato cultivars with Ralstonia solanacearum strain Pss4. We also compared the efficacy of this strain with that of commercial Bacillus subtilis strain Y1336. Strain WF02 provided longer lasting protection against R. solanacearum than did strain Y1336 and controlled the development of wilt in both cultivars. To elucidate the genetic responses in these plants under WF02 treatment, we analyzed the temporal expression of defense-related genes in leaves. The salicylic acid pathway-related genes phenylalanine ammonia-lyase and pathogenesis-related protein 1a were up-regulated in both cultivars, whereas expression of the jasmonic acid pathway-related gene lipoxygenase was only elevated in the susceptible tomato cultivar (L390). These results suggest that WF02 can provide protection against bacterial wilt in tomato cultivars with different levels of disease resistance via direct and indirect modes of action.
authors.affiliation: Master's Program for Plant Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan.
authors.forename: Chu-Ning
authors.initials: CN
authors.lastname: Huang
authors.affiliation: Department of Plant Pathology and Microbiology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan.|Institute of Biotechnology, National Taiwan University, R412., No. 81, Chang-Xing St., Taipei, 106, Taiwan.
authors.forename: Chan-Pin
authors.initials: CP
authors.lastname: Lin
authors.affiliation: Biopesticides Division, Taiwan Agricultural Chemicals and Toxic Substances, Research Institute, Council of Agriculture, No. 11, Guangming Rd., Wufeng, Taichung, 413, Taiwan.
authors.forename: Feng-Chia
authors.initials: FC
authors.lastname: Hsieh
authors.affiliation: Institute of Biotechnology, National Taiwan University, R412., No. 81, Chang-Xing St., Taipei, 106, Taiwan.
authors.forename: Sook-Kuan
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Institute of Biotechnology, National Taiwan University, R412., No. 81, Chang-Xing St., Taipei, 106, Taiwan.|Graduate Institute of Food Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan.|Agricultural Biotechnology Research Center, Academia Sinica, No. 128 Sec. 2, Academia Rd., Nankang, Taipei, 115, Taiwan.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 404, Taiwan.
authors.forename: Kuan-Chen
authors.initials: KC
authors.lastname: Cheng
authors.affiliation: Institute of Biotechnology, National Taiwan University, R412., No. 81, Chang-Xing St., Taipei, 106, Taiwan. chiteliu@ntu.edu.tw.|Agricultural Biotechnology Research Center, Academia Sinica, No. 128 Sec. 2, Academia Rd., Nankang, Taipei, 115, Taiwan. chiteliu@ntu.edu.tw.
authors.forename: Chi-Te
authors.initials: CT
authors.lastname: Liu
mesh_terms: D000069977:Bacillus amyloliquefaciens / Q000302:isolation & purification / Q000502:physiology*; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D018551:Solanum lycopersicum / Q000235:genetics* / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D043368:Ralstonia solanacearum / Q000472:pathogenicity; D012988:Soil Microbiology
chemical_list: D010940:Plant Proteins
keywords: Bacillus amyloliquefaciens; Bacterial wilt; Biocontrol; Induced plant resistance; Ralstonia solanacearum