title: [Inhibitory effect of KyoT2 overexpression on proliferation and migration of airway smooth muscle cells in mice with asthma].
abstract: OBJECTIVE
  To investigate the effect of KyoT2 on the proliferation and migration of airway smooth muscle cells (ASMCs) in mice with asthma.
  
  
  METHODS
  Ovalbumin (OVA) was used to establish the asthmatic model of airway remodeling in BALB/c mice. ASMCs were isolated and cultured, and primarily cultured ASMCs were used as the control group. The expression of KyoT2 in ASMCs was measured in the control and asthma groups. After the ASMCs from asthmatic mice were transfected with pCMV-Myc (empty vector group) or pCMV-Myc-KyoT2 plasmid with overexpressed KyoT2 (KyoT2 expression group) for 48 hours, RT-PCR and Western blot were used to measure the mRNA and protein expression of KyoT2, the MTT assay and BrdU assay were used to measure the proliferation of ASMCs, and Transwell assay was used to measure the migration of ASMCs. Western blot was used to determine the effect of KyoT2 overexpression on the protein expression of RBP-Jκ, PTEN, and AKT.
  
  
  RESULTS
  Compared with the control group, the asthma group had significantly downregulated expression of KyoT2 in ASMCs, and the KyoT2 expression group had significantly upregulated expression of KyoT2 in ASMCs (P<0.05). Compared with the empty vector group, overexpressed KyoT2 significantly inhibited cell proliferation and migration, downregulated the expression of RBP-Jκ and AKT, and upregulated the expression of PTEN.
  
  
  CONCLUSIONS
  Overexpressed KyoT2 can inhibit the proliferation and migration of ASMCs through the negative regulation of RBP-Jκ/PTEN/AKT signaling pathway.
authors.affiliation: Department of Asthma, Children's Hospital of Xi'an, Xi'an 710003, China. hbo_liu@126.com.
authors.forename: Long
authors.initials: L
authors.lastname: Zhao
authors.forename: Cui-Cui
authors.initials: CC
authors.lastname: Liu
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Shi
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
mesh_terms: D000818:Animals; D001249:Asthma / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D047908:Intracellular Signaling Peptides and Proteins / Q000502:physiology*; D060588:LIM Domain Proteins / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009124:Muscle Proteins / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D051059:PTEN Phosphohydrolase / Q000502:physiology; D014132:Trachea / Q000473:pathology*
chemical_list: C486306:Fhl1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; D009124:Muscle Proteins; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse