title: The Role of Nanoparticle in Brain Permeability: An in-vitro BBB Model.
abstract: Membrane permeability and P-glycoprotein (P-gp) efflux system are regulating factors in the drug brain penetration. Recently, some drug delivery systems have been developed to overcome these limitations. In this study, Metoclopramid has been encapsulated in PLGA nanoparticles using the emulsification/solvent evaporation technique for in-viro evaluation of the effect of PLGA nanoparticles on BBB permeability. Subsequently, prepared nanoparticles were characterized using PCS, TEM, FT-IR, DSC and XRD techniques and in-viro cell permeability of optimum formulation was evaluated using MDCK cell line as BBB model. Data investigation showed that prepared nanoparticles have the entrapment efficiency of 50 %. PCS investigation showed that prepared nanoparticles have an average size of approximately 150 ± 14 nm and a relatively monodisperse distribution. TEM micrographs of the samples showed spherical shape and smooth surface with a particle size of nanometric range. Through DSC thermograms and XRD diffractograms analysis, it was demonstrated that there was no crystalline form of the drug in the loaded formulation. Moreover, our results showed that the greater crossing of metoclopramide in the form of nanoparticle in comparison with the free form. The widely used rhodamine-123 transport assay performed in the MDCK cells demonstrated the presence of P-glycoprotein in this model.
authors.affiliation: Nano Drug Delivery research center, Kermanshah University of Medical Sciences, Kermanshah, Iran. ; Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Kermanshah Universityof Medical Sciences, Kermanshah, Iran.
authors.forename: Niusha
authors.initials: N
authors.lastname: Nikandish
authors.affiliation: Nano Drug Delivery research center, Kermanshah University of Medical Sciences, Kermanshah, Iran. ; Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Kermanshah Universityof Medical Sciences, Kermanshah, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Hosseinzadeh
authors.affiliation: Nano Drug Delivery research center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Hemati Azandaryani
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Katayoun
authors.initials: K
authors.lastname: Derakhshandeh
keywords: Blood Brain Barrier; MDCK cell line; P-glycoprotein; Poly (D.L-lactide co glycolide)