title: The Power of Force: Insights into the Protein Folding Process Using Single-Molecule Force Spectroscopy.
abstract: One of the major challenges in modern biophysics is observing and understanding conformational changes during complex molecular processes, from the fundamental protein folding to the function of molecular machines. Single-molecule techniques have been one of the major driving forces of the huge progress attained in the last few years. Recent advances in resolution of the experimental setups, aided by theoretical developments and molecular dynamics simulations, have revealed a much higher degree of complexity inside these molecular processes than previously reported using traditional ensemble measurements. This review sums up the evolution of these developments and gives an outlook on prospective discoveries.
authors.affiliation: CIC nanoGUNE, San Sebastián 20018, Spain.
authors.forename: Jörg
authors.initials: J
authors.lastname: Schönfelder
authors.affiliation: CIC nanoGUNE, San Sebastián 20018, Spain; IKERBASQUE, Basque Foundation for Science, Bilbao 48013, Spain.
authors.forename: David
authors.initials: D
authors.lastname: De Sancho
authors.affiliation: CIC nanoGUNE, San Sebastián 20018, Spain; IKERBASQUE, Basque Foundation for Science, Bilbao 48013, Spain. Electronic address: r.perezjimenez@nanogune.eu.
authors.forename: Raul
authors.initials: R
authors.lastname: Perez-Jimenez
mesh_terms: D017510:Protein Folding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D000072760:Single Molecule Imaging / Q000379:methods*
chemical_list: D011506:Proteins
keywords: force spectroscopy; protein folding; single-molecule