title: Lyophilized allogeneic bone tissue as an antibiotic carrier.
abstract: The rising number of primary joint replacements worldwide causes an increase of revision surgery of endoprostheses due bacterial infection. Revision surgery using non-cemented implants seems beneficial for the long-term outcome and the use of antibiotic-impregnated bone grafts might control the infection and give a good support for the implant. In this study we evaluated the release of antibiotics from fresh-frozen and lyophilized allogeneic bone grafts. Lyophilized bone chips and fresh frozen bone chips were mixed with gentamicin sulphate, gentamicin palmitate, vancomycin, calcium carbonate/calcium sulphate impregnated with gentamicin sulphate, and calcium carbonate/calcium sulphate bone substitute material impregnated with vancomycin. The efficacy of each preparation was measured by drug release tests and bacterial susceptibility using B. subtilis, S. aureus and methicillin-resistant Staphylococcus aureus. The release of gentamicin from lyophilized bone was similar to the release rate from fresh frozen bone during all the experimental time. That fact might be related to the similar porosity and microstructure of the bone chips. The release of gentamicin from lyophilized and fresh frozen bone was high in the first and second day, decreasing and keeping a low rate until the end of the second week. Depending on the surgical strategy either polymethylmethacrylate or allogeneic bone are able to deliver sufficient concentrations of gentamicin to achieve bacterial inhibition within two weeks after surgery. In case of uncemented revision of joint replacements allogeneic bone is able to deliver therapeutic doses of gentamicin and peak levels immediately after implantation during a fortnight. The use of lyophilized and fresh frozen bone allografts as antibiotic carriers is recommended for prophylaxis of bone infection.
authors.affiliation: Experimental Orthopaedics, Department of Orthopaedic Surgery, Medical University of Innsbruck, Innrain 36, 6020, Innsbruck, Austria. debora.coraca-huber@i-med.ac.at.
authors.forename: Débora C
authors.initials: DC
authors.lastname: Coraça-Huber
authors.affiliation: Experimental Orthopaedics, Department of Orthopaedic Surgery, Medical University of Innsbruck, Innrain 36, 6020, Innsbruck, Austria.
authors.forename: Christoph G
authors.initials: CG
authors.lastname: Ammann
authors.affiliation: Experimental Orthopaedics, Department of Orthopaedic Surgery, Medical University of Innsbruck, Innrain 36, 6020, Innsbruck, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Nogler
authors.affiliation: Division of Hygiene and Medical Microbiology, Medical University Innsbruck, Schöpfstr. 41, 3rd Floor, Innsbruck, Austria.
authors.forename: Manfred
authors.initials: M
authors.lastname: Fille
authors.affiliation: Institute for Infectiology, Clinical Microbiology and Hospital Care, ENDO Clinic Hamburg, Holstenstraße 2, Hamburg, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Frommelt
authors.affiliation: Department of Orthopaedics and Orthopaedic Surgery, Medical University Graz, Auenbruggerplatz 5, Graz, Austria.
authors.forename: Klaus-Dieter
authors.initials: KD
authors.lastname: Kühn
authors.affiliation: Department of Orthopaedics and Orthopaedic Surgery, Medical University of Giessen, Baldingerstraße, Marburg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Fölsch
mesh_terms: D064591:Allografts / Q000737:chemistry / Q000382:microbiology; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D018786:Bone Substitutes / Q000737:chemistry; D016025:Bone Transplantation; D004337:Drug Carriers / Q000737:chemistry*; D005270:Femur Head / Q000737:chemistry* / Q000382:microbiology / Q000637:transplantation*; D005612:Freeze Drying; D005839:Gentamicins / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D019520:Living Donors; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D014184:Transplantation, Homologous; D014640:Vancomycin / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D018786:Bone Substitutes; D004337:Drug Carriers; D005839:Gentamicins; D014640:Vancomycin
keywords: Antibiotics; Bone grafts; Joint infection; Local delivery; Lyophilized bone chips