title: Extracellular matrix-derived hydrogels for dental stem cell delivery.
abstract: Decellularized mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds derived from site-specific homologous tissues to direct cell differentiation. The present study investigated the suitability of hydrogels derived from different source tissues: bone, spinal cord and dentine, as suitable carriers to deliver human apical papilla derived mesenchymal stem cells (SCAP) for spinal cord regeneration. Bone, spinal cord, and dentine ECM hydrogels exhibited distinct structural, mechanical, and biological characteristics. All three hydrogels supported SCAP viability and proliferation. However, only spinal cord and bone derived hydrogels promoted the expression of neural lineage markers. The specific environment of ECM scaffolds significantly affected the differentiation of SCAP to a neural lineage, with stronger responses observed with spinal cord ECM hydrogels, suggesting that site-specific tissues are more likely to facilitate optimal stem cell behavior for constructive spinal cord regeneration. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 319-328, 2017.
authors.affiliation: Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials Unit, Université Catholique de Louvain, Brussels, Belgium.|School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Aiswarya
authors.initials: A
authors.lastname: Viswanath
authors.affiliation: Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials Unit, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Julie
authors.initials: J
authors.lastname: Vanacker
authors.affiliation: Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials Unit, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Loïc
authors.initials: L
authors.lastname: Germain
authors.affiliation: Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials Unit, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Julian G
authors.initials: JG
authors.lastname: Leprince
authors.affiliation: Department of Endodontics, University of Texas Health Science Center at San Antonio, San Antonio-TX, USA.
authors.forename: Anibal
authors.initials: A
authors.lastname: Diogenes
authors.affiliation: School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Shakesheff
authors.affiliation: School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: White
authors.affiliation: Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials Unit, Université Catholique de Louvain, Brussels, Belgium.|Université Catholique de Louvain, Institute of Condensed Matter and Nanosciences, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: des Rieux
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D003771:Dental Papilla / Q000166:cytology / Q000378:metabolism*; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D055153:Stem Cell Niche
chemical_list: D020100:Hydrogels
keywords: ECM hydrogels; bone; dental stem cell delivery; dentine; spinal cord