title: Apigenin Attenuates Oxidative Injury in ARPE-19 Cells thorough Activation of Nrf2 Pathway.
abstract: The current study was aimed at evaluating the therapeutic implication of apigenin and to elucidate the underlying mechanism. Thetert-butyl hydroperoxide (t-BHP) at 200μM was used to induce oxidative stress-associated injury in ARPE-19 cells. Apigenin at concentrations less than 800μM did not cause cytotoxic effects on ARPE-19 cells. Cell viability assay showed that apigenin at 200μM significantly promoted cell survival int-BHP-treated ARPE-19 cells. Additionally, apigenin at 100μM significantly protected ARPE-19 cells fromt-BHP-induced apoptosis. Molecular examinations demonstrated that apigenin at 400μM significantly upregulated the mRNA and protein expression of Nrf2 and stimulated its nuclear translocation in ARPE-19 cells treated with or withoutt-BHP. Apigenin 400μM also significantly elevated the expression of HO-1, NQO1, and GCLM at both mRNA and protein levels in the presence or absence oft-BHP. Furthermore, apigenin at 400μM significantly increased the activities of SOD, CAT, GSH-PX, and T-AOC and reduced the levels of ROS and MDA int-BHP-treated ARPE-19 cells. However, these effects of apigenin were all abolished by being transfected with Nrf2 siRNA. Collectively, our current data indicated that apigenin exerted potent antioxidant properties in ARPE-19 cells challenged witht-BHP, which were dependent on activation of Nrf2 signaling.
authors.affiliation: Department of Ophthalmology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China.
authors.forename: Xinrong
authors.identifier: 0000-0003-2047-7298
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Ophthalmology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Ophthalmology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Ophthalmology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China.
authors.forename: Li
authors.initials: L
authors.lastname: Hang