title: PI3K-GLUT4 Signal Pathway Associated with Effects of EX-B3 Electroacupuncture on Hyperglycemia and Insulin Resistance of T2DM Rats.
abstract: Objectives. To explore electroacupuncture's (EA's) effects on fasting blood glucose (FBG) and insulin resistance of type 2 diabetic mellitus (T2DM) model rats and give a possible explanation for the effects. Method. It takes high fat diet and intraperitoneal injection of streptozotocin (STZ, 30 mg/kg) for model preparation. Model rats were randomly divided into T2DM Model group, EA weiwanxiashu (EX-B3) group, and sham EA group (n = 12/group). EA (2 Hz continuous wave, 2 mA, 20 min/day, 6 days/week, 4 weeks) was applied as intervention. FBG, area under curve (AUC) of oral glucose tolerance test (OGTT), insulin resistance index (HOMA-IR), pancreatic B cell function index (HOMA-B), skeletal muscle phosphorylated phosphatidylinositol-3-kinase (PI3K), glucose transporter 4 (GLUT4), and membrane GLUT4 protein expression were measured. Results. EA weiwanxiashu (EX-B3) can greatly upregulate model rat's significantly reduced skeletal muscle PI3K (Y607) and membrane GLUT4 protein expression (P < 0.01), effectively reducing model rats' FBG and AUC of OGTT (P < 0.01). The effects are far superior to sham EA group. Conclusion. EA weiwanxiashu (EX-B3) can upregulate skeletal muscle phosphorylated PI3K protein expression, to stimulate membrane translocation of GLUT4 and thereby increase skeletal muscle glucose intake to treat T2DM.
authors.affiliation: Department of Qigong and Tuina, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Bing-Yan
authors.identifier: 0000-0002-0525-1576
authors.initials: BY
authors.lastname: Cao
authors.affiliation: School of Acupuncture, Moxibustion, and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Rui
authors.identifier: 0000-0002-9648-9191
authors.initials: R
authors.lastname: Li
authors.affiliation: School of Acupuncture, Moxibustion, and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Huan-Huan
authors.identifier: 0000-0003-4715-654X
authors.initials: HH
authors.lastname: Tian
authors.affiliation: School of Acupuncture, Moxibustion, and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Yan-Jia
authors.identifier: 0000-0001-5235-0350
authors.initials: YJ
authors.lastname: Ma
authors.affiliation: School of Acupuncture, Moxibustion, and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Xiao-Gang
authors.identifier: 0000-0003-1268-2259
authors.initials: XG
authors.lastname: Hu
authors.affiliation: School of Acupuncture, Moxibustion, and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Ning
authors.identifier: 0000-0002-1048-4366
authors.initials: N
authors.lastname: Jia
authors.affiliation: School of Acupuncture, Moxibustion, and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Yue-Ying
authors.identifier: 0000-0002-2263-1677
authors.initials: YY
authors.lastname: Wang