title: Effect of graphene oxide ratio on the cell adhesion and growth behavior on a graphene oxide-coated silicon substrate.
abstract: Control of living cells on biocompatible materials or on modified substrates is important for the development of bio-applications, including biosensors and implant biomaterials. The topography and hydrophobicity of substrates highly affect cell adhesion, growth, and cell growth kinetics, which is of great importance in bio-applications. Herein, we investigate the adhesion, growth, and morphology of cultured breast cancer cells on a silicon substrate, on which graphene oxides (GO) was partially formed. By minimizing the size and amount of the GO-containing solution and the further annealing process, GO-coated Si samples were prepared which partially covered the Si substrates. The coverage of GO on Si samples decreases upon annealing. The behaviors of cells cultured on two samples have been observed, i.e. partially GO-coated Si (P-GO) and annealed partially GO-coated Si (Annealed p-GO), with a different coverage of GO. Indeed, the spreading area covered by the cells and the number of cells for a given culture period in the incubator were highly dependent on the hydrophobicity and the presence of oxygenated groups on GO and Si substrates, suggesting hydrophobicity-driven cell growth. Thus, the presented method can be used to control the cell growth via an appropriate surface modification.
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Jin-Tak
authors.initials: JT
authors.lastname: Jeong
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Mun-Ki
authors.initials: MK
authors.lastname: Choi
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Sim
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Jung-Taek
authors.initials: JT
authors.lastname: Lim
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Gil-Sung
authors.initials: GS
authors.lastname: Kim
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Maeng-Je
authors.initials: MJ
authors.lastname: Seong
authors.affiliation: Department of Semiconductor Science and Technology, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Jung-Hwan
authors.initials: JH
authors.lastname: Hyung
authors.affiliation: Department of Physics and Graphene Research Institute, Sejong University, Seoul 143-747, South Korea.
authors.forename: Keun Soo
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Department of Chemistry, College of Science and Arts, Najran University, Najran-11001, Kingdom of Saudi Arabia.|Promising Centre for Sensors and Electronic Devices (PCSED), Najran University, Najran-11001, Kingdom of Saudi Arabia.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Umar
authors.affiliation: Department of Physics, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Sang-Kwon
authors.initials: SK
authors.lastname: Lee