title: Barley HvPAPhy_a as transgene provides high and stable phytase activities in mature barley straw and in grains.
abstract: The phytase purple acid phosphatase (HvPAPhy_a) expressed during barley seed development was evaluated as transgene for overexpression in barley. The phytase was expressed constitutively driven by the cauliflower mosaic virus 35S-promoter, and the phytase activity was measured in the mature grains, the green leaves and in the dry mature vegetative plant parts left after harvest of the grains. The T2-generation of HvPAPhy_a transformed barley showed phytase activity increases up to 19-fold (29 000 phytase units (FTU) per kg in mature grains). Moreover, also in green leaves and mature dry straw, phytase activities were increased significantly by 110-fold (52 000 FTU/kg) and 57-fold (51 000 FTU/kg), respectively. The HvPAPhy_a-transformed barley plants with high phytase activities possess triple potential utilities for the improvement of phosphate bioavailability. First of all, the utilization of the mature grains as feed to increase the release of bio-available phosphate and minerals bound to the phytate of the grains; secondly, the utilization of the powdered straw either directly or phytase extracted hereof as a supplement to high phytate feed or food; and finally, the use of the stubble to be ploughed into the soil for mobilizing phytate-bound phosphate for plant growth.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science and Technology, Research Centre Flakkebjerg, Aarhus University, Slagelse, Denmark.
authors.forename: Inger Baeksted
authors.initials: IB
authors.lastname: Holme
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science and Technology, Research Centre Flakkebjerg, Aarhus University, Slagelse, Denmark.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Dionisio
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science and Technology, Research Centre Flakkebjerg, Aarhus University, Slagelse, Denmark.
authors.forename: Claus Krogh
authors.initials: CK
authors.lastname: Madsen
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science and Technology, Research Centre Flakkebjerg, Aarhus University, Slagelse, Denmark.
authors.forename: Henrik
authors.initials: H
authors.lastname: Brinch-Pedersen
mesh_terms: D010832:6-Phytase / Q000235:genetics / Q000378:metabolism*; D002523:Edible Grain / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D001467:Hordeum / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010833:Phytic Acid / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D010710:Phosphates; D010940:Plant Proteins; D010833:Phytic Acid; D010832:6-Phytase
keywords: HvPAPhy_a phytase; barley; genetic transformation; grain; straw