title: Maternally derived anti-fibroblast growth factor 23 antibody as new tool to reduce phosphorus requirement of chicks.
abstract: Novel means to reduce phosphate input into poultry feeds and increase its retention would preserve world phosphate reserves and reduce environmental impact of poultry production. Here we show that a maternally derived antibody to a fibroblast growth factor-23 (FGF-23) peptide (GMNPPPYS) alleviated phosphorus deficiency in chicks fed low non-phytate phosphorus (nPP) diets. White Leghorn laying hens were vaccinated with either an adjuvant control or the synthetic FGF-23 peptide, and chicks with control or anti-FGF-23 maternal antibodies were fed a diet containing either 0.13 or 0.45% nPP (experiment 1), and 0.20 or 0.45% nPP (experiment 2) for 14 d. In both experiments, decreasing nPP from 0.45 to 0.13 or 0.20% decreased BW gain, G:F, excreta phosphorus, plasma phosphate, and plasma FGF-23 at all time periods examined (nPP main effect, P < 0.05). In experiment 1, chicks with maternal anti-FGF-23 antibody had increased tibiotarsi ash (d 14), and decreased excreta phosphate (d 7, 14) and plasma intact parathyroid hormone (d 7) when compared to chicks with control antibody (antibody main effect, P < 0.05). Mortality (d 7 to 14, 1 to 14), posture scores (d 7, 14) and bone lesion scores (d 14) decreased and plasma phosphate (d 14) increased in anti-FGF-23 chicks fed 0.13% nPP, compared to those with control antibody on the same diet (P < 0.05). In experiment 2, chicks with maternal anti-FGF-23 antibody had increased tibiotarsi ash (d 14), and plasma phosphate (d 14) and 1,25(OH)2D3 (d 14) levels, compared to chicks with control antibody (antibody main effect, P < 0.05). BW gain and G:F were increased in chicks with anti-FGF-23 antibody fed 0.20% nPP, compared to control antibody chicks on the same diet, at all time periods examined (P < 0.05). In conclusion, maternally-derived anti-FGF-23 antibody increased phosphorus retention in chicks fed diets containing either 0.13 or 0.20% nPP and thereby, reduced signs of phosphorus deficiency.
authors.affiliation: Department of Animal Sciences, 1675 Observatory Drive, University of Wisconsin-Madison, Madison, WI 53706, USA.|Institute of Animal Nutrition, 211 Huimin Road, Sichuan Agricultural University, Wenjiang District, Chengdu, Sichuan 611130, China.
authors.forename: Zhouzheng
authors.initials: Z
authors.lastname: Ren
authors.affiliation: Department of Animal Sciences, 1675 Observatory Drive, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Bütz
authors.affiliation: Department of Animal Sciences, 1675 Observatory Drive, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Jordan M
authors.initials: JM
authors.lastname: Sand
authors.affiliation: Department of Animal Sciences, 1675 Observatory Drive, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Cook
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002645:Chickens; D004032:Diet / Q000662:veterinary; D005260:Female; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000276:immunology*; D009751:Nutritional Requirements; D010710:Phosphates / Q000172:deficiency*; D016226:Phosphorus, Dietary / Q000378:metabolism*
chemical_list: D000906:Antibodies; D010710:Phosphates; D016226:Phosphorus, Dietary; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23
keywords: antibody; chick; fibroblast growth factor 23; phosphorus; requirement