title: Demonstration of homologous recombination events in the evolution of bovine viral diarrhoea virus by in silico investigations.
abstract: Complete genome sequences of bovine viral diarrhoea virus types 1 and 2 (BVDV-1 and 2) deposited in the GenBank were submitted to bioinformatic analysis using a recombination-detecting software. The results indicate that recombination events are not rare in the case of BVDV, which frequently causes immunotolerance and, consequently, persistent infection in calves. The lack of specific immunity provides an ideal possibility for multiple infections by antigenically related but genetically different BVDV strains, and hence recombinations may occur. Among the 62 BVDV-1 genomes five recombinants and their possible parent strains, while among the 50 BVDV-2 genomes one simple recombinant and its parent strains were identified, which were supported by extremely strong probability values (P values varying between 1.26 × 10-4and 1.58 × 10-310). Besides the newly identified recombinants, recombination events described previously were confirmed, but in some of these cases former information was completed with new data, or different parent(s) were suggested by the programme (RDP 4.46 BETA) used in this study.
authors.affiliation: Department of Pharmacology and Toxicology, University of Veterinary Medicine , István u. 2, H-1078 Budapest , Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Kővágó
authors.affiliation: National Food Chain Safety Office, Veterinary Diagnostic Directorate , Budapest , Hungary.
authors.forename: Ákos
authors.initials: Á
authors.lastname: Hornyák
authors.affiliation: Heart and Vascular Centre, Semmelweis University , Budapest , Hungary.
authors.forename: Violetta
authors.initials: V
authors.lastname: Kékesi
authors.affiliation: Department of Pathology, University of Veterinary Medicine , István u. 2, H-1078 Budapest , Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Rusvai
mesh_terms: D003198:Computer Simulation; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics*; D019143:Evolution, Molecular; D014644:Genetic Variation; D016679:Genome, Viral; D008957:Models, Genetic; D016865:Reassortant Viruses; D011995:Recombination, Genetic
keywords: BVDV; Genomic recombination; RNA; bovine viral diarrhoea virus