title: Association of Adiponectin Gene Polymorphisms With the Risk of Coronary Artery Disease in Patients With Nonalcoholic Fatty Liver Disease in a Chinese Han Population.
abstract: BACKGROUND
  Cardiovascular events are an independent risk factor for nonalcoholic fatty liver disease (NAFLD), which is the leading cause of mortality in NAFLD patients. Several recent studies demonstrated that adiponectin (Ad) polymorphisms were involved in the progression of NAFLD and coronary artery disease (CAD). However, reports on the association between Ad polymorphisms and the risk of developing CAD in NAFLD patients are lacking in a Northern Han Chinese population.
  
  
  OBJECTIVE
  The present study was designed to evaluate the association between Ad gene polymorphisms (rs266729 and rs2241766) and the risk of developing CAD in Northern Han Chinese patients with NAFLD.
  
  
  METHODS
  In this case-control study, using the polymerase chain reaction (PCR), Adrs266729 and rs2241766 gene polymorphisms were genotyped in B-type ultrasonography-proven NAFLD patients, with (n = 246) or without (n = 247) CAD and in healthy controls (n = 304). Serum lipid profiles were determined using biochemical methods. Statistical analyses were performed using SPSS 17.0 statistical software.
  
  
  RESULTS
  There were significant differences in the Adrs266729 G allele between the NAFLD patients with and without CAD (P < 0.05). In addition, there was a significant difference in the Adrs2241766 G allele of the NAFLD patients compared with that of the controls (P < 0.05). In the NAFLD CAD population, carriers of the G allele of Adrs266729 had higher serum triglycerides (TG), total cholesterol (TC), fasting plasma glucose (FPG), and low-density lipoprotein (LDL) levels and a lower Ad level than their noncarrier counterparts (P = 0.031, P = 0.034, P = 0.007, P < 0.001, and P < 0.001, respectively). NAFLD patients without CAD had higher TG and serum FPG values and a lower Ad level than their noncarrier counterparts (P = 0.014, P = 0.038, and P < 0.001, respectively). In the NAFLD patients with/without CAD, the carriers of the G allele of Adrs2241766 had higher TG levels (P = 0.039 and P = 0.042, respectively) than those of their noncarrier counterparts.
  
  
  CONCLUSIONS
  In this Northern Chinese Han population, the Adrs266729 and rs2241766 G alleles were closely associated with the occurrence of NAFLD. However, only NAFLD patients who carried the Adrs266729 G allele had an increased risk of developing CAD.
authors.affiliation: Medical College of Qingdao University, Qingdao, China; Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, China.
authors.forename: Shui-Xian
authors.initials: SX
authors.lastname: Du
authors.affiliation: Digestive Disease Key Laboratory of Qingdao, Qingdao, China; Central Laboratories, Qingdao Municipal Hospital, Qingdao, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Lu
authors.affiliation: Medical College of Qingdao University, Qingdao, China; Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, China; Digestive Disease Key Laboratory of Qingdao, Qingdao, China.
authors.forename: Quan-Jiang
authors.initials: QJ
authors.lastname: Dong
authors.affiliation: Medical College of Qingdao University, Qingdao, China; Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, China; Digestive Disease Key Laboratory of Qingdao, Qingdao, China.
authors.forename: Shi-Ying
authors.initials: SY
authors.lastname: Xuan
authors.affiliation: Medical College of Qingdao University, Qingdao, China; Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, China; Digestive Disease Key Laboratory of Qingdao, Qingdao, China.
authors.forename: Yong-Ning
authors.initials: YN
authors.lastname: Xin
keywords: Adiponectin; Coronary Artery Disease; Genetic; Nonalcoholic Fatty Liver Disease; Polymorphisms