title: Derivatization in Capillary Electrophoresis.
abstract: Capillary electrophoresis is a well-established separation technique in analytical research laboratories worldwide. Its interesting advantages make CE an efficient and potent alternative to other chromatographic techniques. However, it is also recognized that its main drawback is the relatively poor sensitivity when using optical detection. One way to overcome this limitation is to perform a derivatization reaction which is intended to provide the analyte more suitable analytical characteristics enabling a high sensitive detection. Based on the analytical step where the CE derivatization takes place, it can be classified as precapillary (before separation), in-capillary (during separation), or postcapillary (after separation). This chapter describes the application of four different derivatization protocols (in-capillary and precapillary modes) to carry out the achiral and chiral analysis of different compounds in food and biological samples with three different detection modes (UV, LIF, and MS).
authors.affiliation: Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, University of Alcalá, Ctra. Madrid-Barcelona Km. 33.600, Alcalá de Henares, Madrid, 28871, Spain.
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Marina
authors.affiliation: Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, University of Alcalá, Ctra. Madrid-Barcelona Km. 33.600, Alcalá de Henares, Madrid, 28871, Spain. maria.castrop@uah.es.
authors.forename: María
authors.initials: M
authors.lastname: Castro-Puyana
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D000596:Amino Acids; D016650:Fluorescein-5-isothiocyanate
keywords: Amino acids; Biological samples; Butanol; Capillary electrophoresis; Derivatization; Fluorescein isothiocyanate (FITC); Food samples; In-capillary; Microwave-accelerated derivatization; O-phthalaldehyde (OPA); Precapillary; Ultrasound-accelerated derivatization