title: Expression and promoter analysis of the OsHSP16.9C gene in rice.
abstract: Small heat shock proteins (sHSPs) are molecular chaperones important for stress tolerance. In this study, heat induction of a rice sHSP gene OsHSP16.9C is analyzed to understand the molecular mechanisms underlying gene expression regulation in heat shock responses in rice. Promoter deletion analysis of the OsHSP16.9C using the luciferase (LUC) reporter gene in transgenic rice identifies a critical role of a promoter fragment containing an imperfect heat shock element (HSE) in heat induction. HSE was shown to be important for heat induction of AtHSP18.2, a homolog of OsHSP16.9C in Arabidopsis. In addition, the rice OsHSP16.9C promoter confers heat induction of the reporter gene expression in Arabidopsis. These data suggest that the heat induction mechanisms of OsHSP16.9C and AtHSP18.2 are similar in rice and Arabidopsis. The transgenic reporter line generated offers a system to genetically dissect signaling events in heat induction in rice.
authors.affiliation: The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Baohong
authors.initials: B
authors.lastname: Zou
authors.affiliation: The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Lu
authors.affiliation: The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ding
authors.affiliation: The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: He
authors.initials: H
authors.lastname: Liu
authors.affiliation: The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China; Plant Biology Section, School of Integrated Plant Science, Cornell University, Ithaca, NY 14853, USA. Electronic address: jh299@njau.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Hua
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D006360:Heat-Shock Proteins / Q000235:genetics*; D050888:Heat-Shock Proteins, Small / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response / Q000235:genetics; D006358:Hot Temperature; D009154:Mutation; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; C446287:HSP18.2 protein, Arabidopsis; D006360:Heat-Shock Proteins; D050888:Heat-Shock Proteins, Small; D010940:Plant Proteins
keywords: HSE; Heat-shock response; Rice; Small HSP