title: Gene expression and silencing of activin receptor type 2A (ACVR2A) in myoblast cells of chicken.
abstract: Activin receptor type 2A (ACVR2A) acts as receptor for myostatin (MSTN) protein involved in inhibiting satellite cell proliferation and differentiation. The importance of the ACVR2A gene during embryonic and post-hatch periods in broiler and layer chicken was studied in an in vitro cell culture system. The expression pattern of the ACVR2A gene during embryonic stages was similar in broiler and layer lines. Post-hatch expression of the ACVR2A gene varied significantly between broiler and layer lines. Five shRNA molecules were designed to knockdown expression of the ACVR2A gene in chicken myoblast cells. The silencing of the ACVR2A gene in a cell culture system varied from 60% to 82%. It is concluded that between broiler and layer lines, there were no significant changes in expression of the ACVR2A gene during embryonic stages but it varied significantly during the post-hatch period. The shRNA showed silencing of the ACVR2A gene under an in vitro cell culture system.
authors.affiliation: b ICAR-Indian Veterinary Research Institute , Bareilly , India.
authors.forename: P
authors.initials: P
authors.lastname: Satheesh
authors.affiliation: a ICAR-Directorate of Poultry Research , Hyderabad , India.
authors.forename: T K
authors.initials: TK
authors.lastname: Bhattacharya
authors.affiliation: b ICAR-Indian Veterinary Research Institute , Bareilly , India.
authors.forename: P
authors.initials: P
authors.lastname: Kumar
authors.affiliation: a ICAR-Directorate of Poultry Research , Hyderabad , India.
authors.forename: R N
authors.initials: RN
authors.lastname: Chatterjee
authors.affiliation: b ICAR-Indian Veterinary Research Institute , Bareilly , India.
authors.forename: S K
authors.initials: SK
authors.lastname: Dhara
authors.affiliation: a ICAR-Directorate of Poultry Research , Hyderabad , India.
authors.forename: C
authors.initials: C
authors.lastname: Paswan
authors.affiliation: a ICAR-Directorate of Poultry Research , Hyderabad , India.
authors.forename: R
authors.initials: R
authors.lastname: Shukla
authors.affiliation: a ICAR-Directorate of Poultry Research , Hyderabad , India.
authors.forename: K
authors.initials: K
authors.lastname: Dushyanth
mesh_terms: D030301:Activin Receptors, Type II / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002642:Chick Embryo / Q000378:metabolism; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D020868:Gene Silencing; D032446:Myoblasts / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D030301:Activin Receptors, Type II; C425585:activin receptor type II-A