title: Chemical Constituents from Flueggea virosa and the Structural Revision of Dehydrochebulic Acid Trimethyl Ester.
abstract: In an attempt to study the chemical constituents from the twigs and leaves of Flueggea virosa, a new terpenoid, 9(10→20)-abeo-ent-podocarpane, 3β,10α-dihydroxy-12-methoxy-13- methyl-9(10→20)-abeo-ent-podocarpa-6,8,11,13-tetraene (1), as well as five known compounds were characterized. Their structures were elucidated on the basis of spectroscopic analysis. In addition, the structure of dehydrochebulic acid trimethyl ester was revised as (2S,3R)-4E-dehydrochebulic acid trimethyl ester based on a single-crystal X-ray diffraction study. The in vitro anti-hepatitis C virus (anti-HCV) activity and cytotoxicity against Huh7.5 cells for the isolated compounds were evaluated.
authors.affiliation: School of Pharmacy, China Medical University, Taichung 40402, Taiwan. chaochihhua@hotmail.com.|Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40447, Taiwan. chaochihhua@hotmail.com.
authors.forename: Chih-Hua
authors.initials: CH
authors.lastname: Chao
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan. yjlin@mail.cmu.edu.tw.|Genetic Center, Department of Medical Research, China Medical University Hospital, Taichung 40447, Taiwan. yjlin@mail.cmu.edu.tw.
authors.forename: Ying-Ju
authors.initials: YJ
authors.lastname: Lin
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 40402, Taiwan. jccheng@mail.cmu.edu.tw.
authors.forename: Ju-Chien
authors.initials: JC
authors.lastname: Cheng
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan. hchuang@mail.cmu.edu.tw.
authors.forename: Hui-Chi
authors.initials: HC
authors.lastname: Huang
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 40402, Taiwan. star740101@gmail.com.
authors.forename: Yung-Ju
authors.initials: YJ
authors.lastname: Yeh
authors.affiliation: Department of Pharmacy, National Cheng Kung University, Tainan 70101, Taiwan. tswu@mail.ncku.edu.tw.|Department of Pharmacy and Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung 90741, Taiwan. tswu@mail.ncku.edu.tw.
authors.forename: Tian-Shung
authors.initials: TS
authors.lastname: Wu
authors.affiliation: Endemic Species Research Institute, Council of Agriculture, Nantou 55244, Taiwan. son757780@yahoo.com.tw.
authors.forename: Syh-Yuan
authors.initials: SY
authors.lastname: Hwang
authors.affiliation: School of Pharmacy, China Medical University, Taichung 40402, Taiwan. yachwu@mail.cmu.edu.tw.|Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40447, Taiwan. yachwu@mail.cmu.edu.tw.|Center for Molecular Medicine, China Medical University Hospital, Taichung 40447, Taiwan. yachwu@mail.cmu.edu.tw.|Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. yachwu@mail.cmu.edu.tw.
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D019665:Euphorbiaceae / Q000737:chemistry*; D016174:Hepacivirus; D006526:Hepatitis C / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D008298:Maleates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D001578:Benzopyrans; D008298:Maleates; C087785:dehydrochebulic acid trimethyl ester
keywords: Flueggea virosa; HCVcc; anti-HCV; dehydrochebulic acid trimethyl ester; ent-podocarpane