title: The chemokines secretion and the oxidative stress are targets of low-level laser therapy in allergic lung inflammation.
abstract: Recent studies show that low-level laser therapy (LLLT) has an important anti-inflammatory action in acute lung inflammation. The present work explored if laser therapy is able to antagonize eosinophils and allergic inflammation induced by oxidative stress in Balb/c mice. Forty-eight hours after challenge, the leukocyte counting, ROS and nitrite/nitrate level, RANTES, CCL3, CCL8 as well as eotaxins were measured in the bronchoalveolar lavage fluid (BALF) of laser-treated mice or not. Into the lung, some chemokines receptors, the iNOS activity and mRNA expression, and the activities of superoxide dismutase (SOD), catalase, gluthatione, NADPH oxidase activities and thiobarbituric acid reactive species (T-Bars) were measured. Laser-treated allergic mice presented reduction of both the ICAM-1 and eosinophil in the lungs. RANTES, CCL8, CCL3 and eotaxins were reduced in BALF of laser-treated allergic mice. In allergic mice lung LLLT decreased the CCR1 and CCR3 and restored the oxidative stress balance as well. Laser decreased the lipidic peroxidation in allergic mice lung as much as increased SOD, GPx and GR. It shows that LLLT on allergic lung inflammation involves leukocyte-attractant chemokines and endogenous antioxidant. Based on results, LLLT may ultimately become a non- invasive option in allergic lung disease treatment. The top figure illustrates the laser decreasing the eosinophils migration into BALF and the bottom figure shows the laser upregulating the expression of heme-oxygenase (anti-oxidant enzyme) in lung tissue anti-oxidant.
authors.affiliation: Department of Science and Technology, Federal University of São Paulo - UNIFESP, Rua Talim, 330 - Vila Nair, PO Box 12231-280, São José dos Campos, São Paulo, SP, Brazil.
authors.forename: Jorge Luis
authors.initials: JL
authors.lastname: Costa Carvalho
authors.affiliation: Laboratory of Pulmonary and Exercise Immunology-LABPEI, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.
authors.forename: Auriléia Aparecida
authors.initials: AA
authors.lastname: de Brito
authors.affiliation: Laboratory of Pulmonary and Exercise Immunology-LABPEI, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.
authors.forename: Ana Paula Ligeiro
authors.initials: AP
authors.lastname: de Oliveira
authors.affiliation: Laboratory of Immunopharmacology, Oswaldo Cruz Foundation - FioCruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Hugo Caire
authors.initials: HC
authors.lastname: de Castro Faria Neto
authors.affiliation: Laboratory of Pulmonary and Exercise Immunology-LABPEI, Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.
authors.forename: Thiago Martini
authors.initials: TM
authors.lastname: Pereira
authors.affiliation: Department of Science and Technology, Federal University of São Paulo - UNIFESP, Rua Talim, 330 - Vila Nair, PO Box 12231-280, São José dos Campos, São Paulo, SP, Brazil.
authors.forename: Regiane Albertini
authors.initials: RA
authors.lastname: de Carvalho
authors.affiliation: Department of Science and Technology, Federal University of São Paulo - UNIFESP, Rua Talim, 330 - Vila Nair, PO Box 12231-280, São José dos Campos, São Paulo, SP, Brazil.
authors.forename: Elen
authors.initials: E
authors.lastname: Anatriello
authors.affiliation: Department of Science and Technology, Federal University of São Paulo - UNIFESP, Rua Talim, 330 - Vila Nair, PO Box 12231-280, São José dos Campos, São Paulo, SP, Brazil.
authors.forename: Flávio
authors.initials: F
authors.lastname: Aimbire
mesh_terms: D000818:Animals; D001249:Asthma / Q000532:radiotherapy*; D018925:Chemokines / Q000378:metabolism*; D007249:Inflammation / Q000532:radiotherapy*; D028022:Low-Level Light Therapy; D008168:Lung / Q000503:physiopathology / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress
chemical_list: D018925:Chemokines
keywords: asthma; chemokines; eosinophils; low-level laser therapy; oxidative stress; photobiomodulation