title: Coronin 1B supports RhoA signaling at cell-cell junctions through Myosin II.
abstract: Non-muscle myosin II (NMII) motor proteins are responsible for generating contractile forces inside eukaryotic cells. There is also a growing interest in the capacity for these motor proteins to influence cell signaling through scaffolding, especially in the context of RhoA GTPase signaling. We previously showed that NMIIA accumulation and stability within specific regions of the cell cortex, such as the zonula adherens (ZA), allows the formation of a stable RhoA signaling zone. Now we demonstrate a key role for Coronin 1B in maintaining this junctional pool of NMIIA, as depletion of Coronin 1B significantly compromised myosin accumulation and stability at junctions. The loss of junctional NMIIA, upon Coronin 1B knockdown, perturbed RhoA signaling due to enhanced junctional recruitment of the RhoA antagonist, p190B Rho GAP. This effect was blocked by the expression of phosphomimetic MRLC-DD, thus reinforcing the central role of NMII in regulating RhoA signaling.
authors.affiliation: a Division of Cell Biology and Molecular Medicine, Program in Membrane Interface Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia , Brisbane , Queensland , Australia.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Priya
authors.affiliation: a Division of Cell Biology and Molecular Medicine, Program in Membrane Interface Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia , Brisbane , Queensland , Australia.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Wee
authors.affiliation: a Division of Cell Biology and Molecular Medicine, Program in Membrane Interface Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia , Brisbane , Queensland , Australia.
authors.forename: Srikanth
authors.initials: S
authors.lastname: Budnar
authors.affiliation: a Division of Cell Biology and Molecular Medicine, Program in Membrane Interface Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia , Brisbane , Queensland , Australia.
authors.forename: Guillermo A
authors.initials: GA
authors.lastname: Gomez
authors.affiliation: a Division of Cell Biology and Molecular Medicine, Program in Membrane Interface Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia , Brisbane , Queensland , Australia.
authors.forename: Alpha S
authors.initials: AS
authors.lastname: Yap
authors.affiliation: a Division of Cell Biology and Molecular Medicine, Program in Membrane Interface Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia , Brisbane , Queensland , Australia.|b Randall Division of Cell and Molecular Biophysics, King's College London, Guy's Campus , London , UK.
authors.forename: Magdalene
authors.initials: M
authors.lastname: Michael
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D000205:Actomyosin / Q000378:metabolism; D022005:Adherens Junctions / Q000378:metabolism; D018938:Caco-2 Cells; D015820:Cadherins / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D007365:Intercellular Junctions / Q000378:metabolism*; D061986:MCF-7 Cells; D008954:Models, Biological; D018994:Myosin Light Chains / Q000378:metabolism; D024507:Nonmuscle Myosin Type IIA / Q000378:metabolism*; D010641:Phenotype; D055550:Protein Stability; D015398:Signal Transduction; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*; D054378:Acetogenins
chemical_list: D015107:4-Butyrolactone; D000205:Actomyosin; D015820:Cadherins; D020690:GTPase-Activating Proteins; D006160:Guanosine Triphosphate; D018994:Myosin Light Chains; D024507:Nonmuscle Myosin Type IIA; D020742:rhoA GTP-Binding Protein; C438920:coronin acetogenin; D054378:Acetogenins
keywords: Coronin 1B; Feedback; NMIIA; RhoA; p190B