title: Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana.
abstract: The Cucumber mosaic virus (CMV) suppressor 2b co-localizes with AGO4 in cytoplasmic and nuclear fractions of Arabidopsis thaliana Biochemical fractionation of A. thaliana cellular extracts revealed that 2b and AGO4 coexist in multiple size exclusions. 2b transgenic A. thaliana exhibited an enhanced accumulation of 24nt siRNAs from flowering wageningen (FWA) and other heterochromatic loci. These plants also exhibited hypo-methylation of an endogenous- as well as transgene-FWA promoter at non-CG sites. In corroboration, both transgenic 2b and CMV infection affected the regulation of transposons which mimics the ago4 phenotype. In conclusion, 2b perturbs plant defense by interfering with AGO4-regulated transcriptional gene silencing.
authors.affiliation: SBA School of Science and Engineering, Lahore University of Management Sciences (LUMS), DHA, Lahore 54792, Pakistan fangrx@im.ac.cn chenxy@im.ac.cn hamera2010@gmail.com.|State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Sadia
authors.initials: S
authors.lastname: Hamera
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Youngsheng
authors.initials: Y
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Song
authors.affiliation: SBA School of Science and Engineering, Lahore University of Management Sciences (LUMS), DHA, Lahore 54792, Pakistan.
authors.forename: Safee Ullah
authors.initials: SU
authors.lastname: Chaudhary
authors.affiliation: Department of Biochemistry, Quaid i Azam University, Islamabad 54320, Pakistan.
authors.forename: Iram
authors.initials: I
authors.lastname: Murtaza
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Su
authors.affiliation: SBA School of Science and Engineering, Lahore University of Management Sciences (LUMS), DHA, Lahore 54792, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Tariq
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China fangrx@im.ac.cn chenxy@im.ac.cn hamera2010@gmail.com.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China fangrx@im.ac.cn chenxy@im.ac.cn hamera2010@gmail.com.
authors.forename: Rongxiang
authors.identifier: http://orcid.org/0000-0002-1677-4591
authors.initials: R
authors.lastname: Fang
keywords: AGO4; CMV2b; Cucumber mosaic virus; FWA; RdDM