title: Targeted Tumor Therapy Based on Nanodiamonds Decorated with Doxorubicin and Folic Acid.
abstract: The fabrication of nanodiamond (ND)-based drug carriers for tumor-targeted drug delivery is described. The ND clusters with an average size of 52.84 nm are fabricated using a simple fluidic device combined with a precipitation method and then conjugated with folic acid (FA) and doxorubicin (Dox) via carbodiimide chemistry to obtain FA/Dox-modified ND (FA/Dox-ND) clusters. Cell culture experiments revealed that KB (folate receptor-positive) cells are preferentially ablated by FA/Dox-ND clusters compared to A549 (folate receptor-negative) cells. In vivo results revealed that FA/Dox-ND clusters are specifically accumulated in tumor tissues after intravenous injection into tumor-bearing mice, effectively reducing the volume of tumor. Based on these results, this study suggests that FA/Dox-ND clusters can be a good candidate as tumor-targeted nanovehicles for delivery of antitumor drug.
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 420-743, Republic of Korea.
authors.forename: Tae-Kyung
authors.initials: TK
authors.lastname: Ryu
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 420-743, Republic of Korea.
authors.forename: Seung Woon
authors.initials: SW
authors.lastname: Baek
authors.affiliation: Nuclear Materials Research Division, Korea Atomic Energy Research Institute, 1045 Daedeok Daero, Yuseong-gu, Daejeon, 305-353, Republic of Korea.
authors.forename: Gyoung-Ja
authors.initials: GJ
authors.lastname: Lee
authors.affiliation: Nuclear Materials Research Division, Korea Atomic Energy Research Institute, 1045 Daedeok Daero, Yuseong-gu, Daejeon, 305-353, Republic of Korea.
authors.forename: Chang-Kyu
authors.initials: CK
authors.lastname: Rhee
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 420-743, Republic of Korea.
authors.forename: Sung-Wook
authors.initials: SW
authors.lastname: Choi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology / Q000627:therapeutic use*; D004705:Endocytosis / Q000187:drug effects; D005492:Folic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D058990:Molecular Targeted Therapy; D058612:Nanodiamonds / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010316:Particle Size; D055672:Static Electricity
chemical_list: D058612:Nanodiamonds; D004317:Doxorubicin; D005492:Folic Acid
keywords: doxorubicin, folic acid; nanodiamond; targeted tumor therapy