title: Functionalized magnetic microparticle-based colorimetric platform for influenza A virus detection.
abstract: A colorimetric platform for influenza A virus detection was developed by using the high efficiency of enzymatic catalysis and the reduction of gold ions with hydrogen peroxide. Aptamer-functionalized magnetic microparticles were synthesized to capture the influenza A virus. This was followed by the binding of ConA-GOx-AuNPs to the H3N2 virus through the ConA-glycan interaction. The sandwich complex was subsequently dispersed in glucose solution to trigger an enzymatic reaction to produce hydrogen peroxide, which controlled the growth of gold nanoparticles and produced colored solutions. The determination of H3N2 concentration was realized by comparing the two differently colored gold nanoparticles. This method could detect the target virus as low as 11.16 μg ml(-1). Furthermore, it opens new opportunities for sensitive and colorimetric detection of viruses and proteins.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China. Institute for Interdisciplinary Research & Key Laboratory of Optoelectronic Chemical Materials and Devices, Ministry of Education, Jianghan University, Wuhan 430056, People's Republic of China.
authors.forename: Chaohui
authors.initials: C
authors.lastname: Chen
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zou
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Xinghu
authors.initials: X
authors.lastname: Ji
authors.forename: Zhike
authors.initials: Z
authors.lastname: He
mesh_terms: D015374:Biosensing Techniques; D003124:Colorimetry; D006046:Gold; D053122:Influenza A Virus, H3N2 Subtype; D053768:Metal Nanoparticles
chemical_list: D006046:Gold