title: Production of recombinant dengue non-structural 1 (NS1) proteins from clinical virus isolates.
abstract: Dengue is a febrile disease caused by infection of dengue virus (DENV). Early diagnosis of dengue infection is important for better management of the disease. The DENV Non-Structural Protein 1 (NS1) antigen has been routinely used for the early dengue detection. In dengue epidemic countries such as Indonesia, clinicians are increasingly relying on the NS1 detection for confirmation of dengue infection. Various NS1 diagnostic tests are commercially available, however different sensitivities and specificities were observed in various settings. This study was aimed to generate dengue NS1 recombinant protein for the development of dengue diagnostic tests. Four Indonesian DENV isolates were used as the source of the NS1 gene cloning, expression, and purification in bacterial expression system. Recombinant NS1 proteins were successfully purified and their antigenicities were assessed. Immunization of mice with recombinant proteins observed the immunogenicity of the NS1 protein. The generated recombinant proteins can be potentially used in the development of NS1 diagnostic test. With minimal modifications, this method can be used for producing NS1 recombinant proteins from isolates obtained from other geographical regions.
authors.affiliation: Eijkman Institute for Molecular Biology, Jl. Diponegoro 69, Jakarta, 10430, Indonesia.
authors.forename: Benediktus
authors.initials: B
authors.lastname: Yohan
authors.affiliation: Clinical Pathology Department, School of Medicine and Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
authors.forename: Puspa
authors.initials: P
authors.lastname: Wardhani
authors.affiliation: Clinical Pathology Department, School of Medicine and Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
authors.lastname: Aryati
authors.affiliation: Eijkman Institute for Molecular Biology, Jl. Diponegoro 69, Jakarta, 10430, Indonesia; Agency for the Assessment and Application of Technology, Jakarta, 10340, Indonesia.
authors.forename: Hidayat
authors.initials: H
authors.lastname: Trimarsanto
authors.affiliation: Eijkman Institute for Molecular Biology, Jl. Diponegoro 69, Jakarta, 10430, Indonesia. Electronic address: sasmono@eijkman.go.id.
authors.forename: R Tedjo
authors.initials: RT
authors.lastname: Sasmono
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins
keywords: Dengue; Diagnostic; NS1; Recombinant protein