title: Preparation of alkyne-labeled 2-nitroimidazoles for identification of tumor hypoxia by Raman spectroscopy.
abstract: Hypoxia is a characteristic feature of solid tumors. Herein, we have developed novel hypoxia-sensitive probes (IM-ACs) for Raman spectroscopic analysis, consisting of nitroimidazole as a hypoxia-targeting unit and acetylene group as the signal-emitting unit. Among IM-ACs synthesized in this study, IM-AC possessing a diacetylene group (IM-AC 3), showed suitable properties as a hypoxia indicator. When administered to A549 cells, we observed a strong signal of IM-AC 3 around 2200cm-1in the Raman spectra from hypoxic cells. Ex vivo experiments suggest that IM-AC 3 remained in hypoxic tumor tissue and emitted a strong signal.
authors.affiliation: Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, 5-10-1 Fuchinobe, Chuo-ku, Sagamihara 252-5258, Japan.
authors.forename: Ryohsuke
authors.initials: R
authors.lastname: Kurihara
authors.affiliation: Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, 5-10-1 Fuchinobe, Chuo-ku, Sagamihara 252-5258, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Ikemura
authors.affiliation: Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, 5-10-1 Fuchinobe, Chuo-ku, Sagamihara 252-5258, Japan. Electronic address: tanabe.kazuhito@chem.aoyama.ac.jp.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Tanabe
mesh_terms: D000072283:A549 Cells; D000480:Alkynes / Q000737:chemistry*; D015687:Cell Hypoxia; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D015335:Molecular Probes; D009593:Nitroimidazoles / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000480:Alkynes; D015335:Molecular Probes; D009593:Nitroimidazoles
keywords: Acetylene; Nitroimidazole; Raman spectroscopy; Tumor hypoxia