title: Effect of high temperature on immune response of grass carp (Ctenopharyngodon idellus) by transcriptome analysis.
abstract: Exposure to high temperature is an inherent feature of grass carp culture in southern China and juvenile grass carps are predisposed to infectious disease in this condition. To understand how high temperature impacts the immune response to pathogens in grass carp, the transcriptomic profiles of the spleens from immune injected grass carp groups undergoing heat stress and normal temperature were investigated. An average of 72 million clean reads per library was obtained, and approximate 80% of these genes were successfully mapped to the reference genome. A total of 2287 up-regulated and 1068 down-regulated genes were identified. 10 immune-related categories involving 90 differently expressed genes were scrutinized. Expression patterns of 18 differentially expressed genes involving in immune response were validated by quantitative real-time RT-PCR. These results provide further significant insights into the influence mechanism of high temperature to immune response in grass carp.
authors.affiliation: College of Life Science, Foshan University, Foshan, Guangdong 528231, China; College of Life Science, South China Normal University, Guangzhou, Guangdong 510631, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Life Science, Foshan University, Foshan, Guangdong 528231, China. Electronic address: 465548936@qq.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.affiliation: College of Life Science, Foshan University, Foshan, Guangdong 528231, China. Electronic address: okhuali@aliyun.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Life Science, South China Normal University, Guangzhou, Guangdong 510631, China.
authors.forename: Anli
authors.initials: A
authors.lastname: Wang
authors.affiliation: College of Life Science, Foshan University, Foshan, Guangdong 528231, China.
authors.forename: Hai-Yi
authors.initials: HY
authors.lastname: Yu
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000818:Animals; D002347:Carps; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation / Q000502:physiology*; D016905:Gram-Negative Bacterial Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D006358:Hot Temperature / Q000009:adverse effects*; D007113:Immunity, Innate; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017423:Sequence Analysis, RNA / Q000662:veterinary
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins
keywords: Ctenopharygodon idella; High temperature; Immune response; Infectious disease; Transcriptomic