title: Glucansucrases produced by fructophilic lactic acid bacteria Lactobacillus kunkeei H3 and H25 isolated from honeybees.
abstract: Twenty fructophilic isolates from the stomachs of honeybee Apis mellifera ligustica from the region of Plovdiv, Bulgaria were obtained. Fructophilic isolates H3 and H25 showed formation of mucous colonies during cultivation on medium with sucrose, suggesting exopolysaccharide synthesis. The sequencing analysis of 16S rRNA identified isolates H3 and H25 as fructophilic lactic acid bacteria Lactobacillus kunkeei. The in situ analysis and periodic acid-Schiff's staining, showed that Lb. kunkeei H3 and H25 produce extracellular glucansucrases with molecular weight of about 300 kDa. In the cell-associated fractions, additional glucansucrase is detected with molecular weight of about 180 kDa. The content of α-(1 → 6) linkages in the glucans synthesized with extracellular glucansucrases from H3 and H25 after dextranase hydrolysis was significantly lower than this one of the classical dextran - about 35 and 62%, respectively. These results suggest a more branched structure of the studied polymers.
authors.affiliation: Department of Biochemistry and Microbiology, Laboratory of Biochemistry, Plovdiv University, Plovdiv, Bulgaria.
authors.forename: Tonka
authors.initials: T
authors.lastname: Vasileva
authors.affiliation: Department of Biochemistry and Microbiology, Laboratory of Biochemistry, Plovdiv University, Plovdiv, Bulgaria.
authors.forename: Veselin
authors.initials: V
authors.lastname: Bivolarski
authors.affiliation: Department of Biochemistry and Microbiology, Laboratory of Biochemistry, Plovdiv University, Plovdiv, Bulgaria.
authors.forename: Galya
authors.initials: G
authors.lastname: Michailova
authors.affiliation: Department of Biochemistry and Microbiology, Laboratory of Biochemistry, Plovdiv University, Plovdiv, Bulgaria.
authors.forename: Ayshe
authors.initials: A
authors.lastname: Salim
authors.affiliation: Department of General and Industrial Microbiology, Sofia University, Sofia, Bulgaria.
authors.forename: Yavor
authors.initials: Y
authors.lastname: Rabadjiev
authors.affiliation: Department of General and Industrial Microbiology, Sofia University, Sofia, Bulgaria.
authors.forename: Iskra
authors.initials: I
authors.lastname: Ivanova
authors.affiliation: Department of Biochemistry and Microbiology, Laboratory of Biochemistry, Plovdiv University, Plovdiv, Bulgaria.
authors.forename: Ilia
authors.initials: I
authors.lastname: Iliev
mesh_terms: D000818:Animals; D001516:Bees / Q000382:microbiology*; D002031:Bulgaria; D004269:DNA, Bacterial; D005632:Fructose / Q000378:metabolism*; D005936:Glucans / Q000008:administration & dosage / Q000737:chemistry; D016695:Glycosyltransferases / Q000096:biosynthesis*; D006868:Hydrolysis; D007778:Lactobacillus / Q000201:enzymology* / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D013270:Stomach / Q000382:microbiology; D013395:Sucrose / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005936:Glucans; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S; D005632:Fructose; D013395:Sucrose; D016695:Glycosyltransferases; C033837:alternansucrase
keywords: Exopolysaccharides; FLAB; Glucans; Glucansucrases; Honeybee