title: Secondary Ion Mass Spectrometry Imaging of Tissues, Cells, and Microbial Systems.
abstract: Secondary ion mass spectrometry (SIMS) is a technique capable of imaging tissues, single cells, and microbes revealing chemical species with sub-micrometer spatial resolution. The recently developed Fourier transform ion cyclotron resonance (FTICR) SIMS instrument provides high mass resolving power and mass accuracy, ToF-SIMS can generate chemical maps with an order of magnitude better lateral resolution than the FTICR-SIMS, and the NanoSIMS instrument offers sub-100 nm spatial resolution in chemical imaging. Many commercial ToF-SIMS instruments are also capable of depth profiling that allows three-dimensional reconstructions of cell and tissue structure.
authors.affiliation: Molecular Engr. And Sci. Bldg, Box 351653, University of Washington (NESACBIO), Seattle, WA 98195, USA.
authors.forename: Lara J
authors.initials: LJ
authors.lastname: Gamble
authors.affiliation: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland, WA 99352, USA.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Anderton