title: The synergistic antiviral effects of GSH in combination with acyclovir against BoHV-1 infection in vitro.
abstract: We have recently reported that bovine herpesvirus 1 (BoHV-1)-induced overproduction of reactive oxygen species (ROS) partially depends on NADPH oxidases (Noxs). In this study, we found that the decreased levels of main intracellular non-enzymatic antioxidant glutathione (GSH) during BoHV-1 infection also contributed to ROS production. Exogenous GSH administration dramatically inhibited BoHV-1 replication, indicating the critical role of decreased GSH for BoHV-1 replication. Interestingly, GSH synergistically enhanced the antiviral effects of acyclovir (ACV) against BoHV-1 infection in vitro. This study not only illuminates the effect of GSH on BoHV-1 infection but also provides evidence that pharmacological modulation of GSH-mediated ROS production in combination with specific antiviral drugs is a viable therapeutic approach to fighting virus infection.
authors.forename: Ch
authors.initials: Ch
authors.lastname: Yuan
authors.forename: X
authors.initials: X
authors.lastname: Fu
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.forename: Y
authors.initials: Y
authors.lastname: Ma
authors.forename: X
authors.initials: X
authors.lastname: Ding
authors.forename: L
authors.initials: L
authors.lastname: Zhu
authors.forename: G
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000212:Acyclovir / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005978:Glutathione / Q000008:administration & dosage / Q000494:pharmacology*; D007242:Herpesvirus 1, Bovine; D061985:Madin Darby Canine Kidney Cells; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D005978:Glutathione; D000212:Acyclovir
keywords: BoHV-1; ROS; GSH; Noxs.