title: MicroRNA-141 inhibits glioma cells growth and metastasis by targeting TGF-β2.
abstract: MicroRNA-141 (miR-141) has been reported to function as tumor suppressor in many types of cancer. However, the molecular function and underlying mechanisms of miR-141 in glioma is still unknown. The aims of this study were to investigate miR-141 expression and determine its biological function and underlying mechanism in glioma. In this study, we found that miR-141 expression levels, both in glioma cell lines and in tissues, were significantly lower than that in a normal human astrocyte cell line or adjacent non-cancerous tissues. Overexpression of miR-141 significantly inhibited glioma cell proliferation, colony formation, migration and invasion in vitro, as well as suppressed glioma tumor growth in vivo. In addition, transforming growth factor beta 2 (TGF-β2) was identified as a target of miR-141 in glioma cells. TGF-β2 expression was also found to be upregulated, and negatively associated with miR-141 in glioma tissues. TGF-β2 over-expression partly reversed the effect caused by transfection of miR-141 mimic. These findings together suggested that miR-141 functioned as tumor suppressor by targeting TGF-β2, and that miR-141 might be a promising therapeutic strategy for future treatment of glioma.
authors.affiliation: Department of Neurosurgery, The First Hospital of Jilin University Changchun 130021, Jilin Province, P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.affiliation: Department of Neurosurgery, The First Hospital of Jilin University Changchun 130021, Jilin Province, P. R. China.
authors.forename: Shuyan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The First Hospital of Jilin University Changchun 130021, Jilin Province, P. R. China.
authors.forename: Wenchen
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, The First Hospital of Jilin University Changchun 130021, Jilin Province, P. R. China.
authors.forename: Shuanglin
authors.initials: S
authors.lastname: Fu
authors.affiliation: Department of Neurosurgery, The First Hospital of Jilin University Changchun 130021, Jilin Province, P. R. China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Luan
authors.affiliation: Department of Ophthalmology, The Second Hospital of Jilin University Changchun 130041, Jilin Province, P. R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zuo
keywords: Glioma; TGF-β2; invasion; miR-141; migration; proliferation