title: In Vitro Evaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract.
abstract: Jaboticaba is a fruit from a native tree to Brazil, Plinia peruviana. Jaboticaba peels are an important source of antioxidant molecules such as phenolic compounds. This study aimed to evaluate in vitro the activity of a hydroalcoholic extract of jaboticaba fruit peels (HEJFP) in wound healing processes and antioxidant activity in murine fibroblasts (L929 cell line). HEJFP concentrations (0.5, 1, 5, 10, 25, 50, 100, and 200 µg/mL) were tested in MTT assay and cell proliferation was verified at 100 µg/mL after 24 h and at 25, 50, and 100 µg/mL after 48 h of extract exposure. Evaluation of antioxidant activity was performed at 0.5, 5, 25, 50, and 100 µg/mL HEJFP concentrations. Cell treatment with HEJFP at 25, 50, and 100 µg/mL for 24 h followed by H2O2 exposure for 3 h showed a strong cytoprotective effect. In vitro scratch wound healing assay indicated that none of tested HEJFP concentrations (0.5, 5, 25, 50, and 100 µg/mL) were capable of increasing migration rate after 12 h of incubation. These results demonstrate a positive effect of HEJFP on the wound healing process on L929 fibroblasts cell line, probably due to the antioxidant activity exhibited by phytochemicals in the extract.
authors.affiliation: Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 1346 Admar Gonzaga Road, 88048-000 Florianópolis, SC, Brazil.
authors.forename: Heloisa da S
authors.identifier: 0000-0002-8308-7740
authors.initials: Hda S
authors.lastname: Pitz
authors.affiliation: Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 1346 Admar Gonzaga Road, 88048-000 Florianópolis, SC, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Pereira
authors.affiliation: Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 1346 Admar Gonzaga Road, 88048-000 Florianópolis, SC, Brazil.
authors.forename: Mayara B
authors.initials: MB
authors.lastname: Blasius
authors.affiliation: Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 1346 Admar Gonzaga Road, 88048-000 Florianópolis, SC, Brazil.
authors.forename: Ana Paula L
authors.initials: AP
authors.lastname: Voytena
authors.affiliation: Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 1346 Admar Gonzaga Road, 88048-000 Florianópolis, SC, Brazil.
authors.forename: Regina C L
authors.identifier: 0000-0003-1987-2696
authors.initials: RC
authors.lastname: Affonso
authors.affiliation: Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 1346 Admar Gonzaga Road, 88048-000 Florianópolis, SC, Brazil.
authors.forename: Simone
authors.initials: S
authors.lastname: Fanan
authors.affiliation: Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 1346 Admar Gonzaga Road, 88048-000 Florianópolis, SC, Brazil.
authors.forename: Adriana C D
authors.initials: AC
authors.lastname: Trevisan
authors.affiliation: Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 1346 Admar Gonzaga Road, 88048-000 Florianópolis, SC, Brazil.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Ribeiro-do-Valle
authors.affiliation: Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 1346 Admar Gonzaga Road, 88048-000 Florianópolis, SC, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Maraschin
mesh_terms: D000975:Antioxidants; D005638:Fruit / Q000737:chemistry*; D027822:Myrtaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts