title: Supermolecules of poly(N-isopropylacrylamide) complexating Herring sperm DNA with bio-multiple hydrogen bonding.
abstract: In this study we used the poly(N-isopropylacrylamide) (PNIPAAm) as a medium to blend with an organic DNA, herring sperm DNA (HSD), to generate PNIPAAm-HSD supramolecular complexes. Bio-multiple hydrogen bonding (BMHB) between PNIPAAm and HSD was investigated that changed the temperature responsiveness of PNIPAAm relatively to the HSD concentrations. With blending the HSD into PNIPAAm matrix, the phase separation in solution is completely opposite from that of neat PNIPAAm. Surface property in static water contact angle (SWCA) is also opposite from that of pure PNIPAAm upon increasing HSD content over 60%. In addition, we found that the PNIPAAm and HSD self-assembled a specific triangle-like structure at a PNIPAAm-to-HSD weight ratio of 1:4 at 25°C; while the triangle-like structure disappeared with increasing temperature to 45°C. Furthermore, both PNIPAAm and HSD could be regarded as insulator, but it transformed into a semiconductive matter after blending with the HSD. Incorporation of organic DNA with hydrogel could significantly change their properties, which might facilitate their use as novel materials in bioelectronics.
authors.affiliation: Department of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan; Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43, Sec 4, Keelung Rd, Taipei 106, Taiwan.
authors.forename: Ai-Wei
authors.initials: AW
authors.lastname: Lee
authors.affiliation: Department of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Cheng-Chen
authors.initials: CC
authors.lastname: Hsu
authors.affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43, Sec 4, Keelung Rd, Taipei 106, Taiwan.
authors.forename: Yi-Zu
authors.initials: YZ
authors.lastname: Liu
authors.affiliation: Cancer Center, Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Department of Surgery, College of Medicine, Graduate Institute of Cancer Biology and Drug Discovery, Taipei Medical University, Taipei, Taiwan.
authors.forename: Po-Li
authors.initials: PL
authors.lastname: Wei
authors.affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43, Sec 4, Keelung Rd, Taipei 106, Taiwan. Electronic address: jkchen@mail.ntust.edu.tw.
authors.forename: Jem-Kun
authors.initials: JK
authors.lastname: Chen
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002152:Calorimetry, Differential Scanning; D004247:DNA / Q000737:chemistry*; D005399:Fishes; D020100:Hydrogels / Q000737:chemistry; D006860:Hydrogen Bonding; D046911:Macromolecular Substances / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D044367:Phase Transition; D013053:Spectrophotometry; D013094:Spermatozoa / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D020100:Hydrogels; D046911:Macromolecular Substances; D014867:Water; C052970:poly-N-isopropylacrylamide; D004247:DNA
keywords: Herring sperm DNA; Poly(N-isopropylacrylamide); Supermolecules