title: Reassortment of high-yield influenza viruses in vero cells and safety assessment as candidate vaccine strains.
abstract: Vaccination is the practiced and accessible measure for preventing influenza infection. Because chicken embryos used for vaccine production have various insufficiencies, more efficient methods are needed. African green monkey kidney (Vero) cells are recommended by the World Health Organization (WHO) as a safe substitute for influenza vaccine production for humans. However, the influenza virus usually had low-yield in Vero cells, which limits the usage of Vero cellular vaccines. This study used 2 high-yield influenza viruses in Vero cells: A/Yunnan/1/2005Va (H3N2) and B/Yunnan/2/2005Va (B) as donor viruses. It used 3 wild strain viruses to reassort new adaptation viruses, including: A/Tianjin/15/2009(H1N1), A/Fujian/196/2009(H3N2), and B/Chongqing/1384/2010(B). These three new viruses could maintain the characteristic of high-yield in Vero cells. Furthermore, they could keep the immunogenic characteristics of the original wild influenza viruses. Importantly, these viruses were shown as safe in chicken embryo and guinea pigs assessment systems. These results provide an alternative method to produce influenza vaccine based on Vero cells.
authors.affiliation: a The Fifth Department of Biological Products , Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College , Kunming , Yunnan Province , People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: a The Fifth Department of Biological Products , Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College , Kunming , Yunnan Province , People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: a The Fifth Department of Biological Products , Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College , Kunming , Yunnan Province , People's Republic of China.|b The First Hospital of Yunnan Province , Kunming , Yunnan Province , People's Republic of China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Yang
authors.affiliation: a The Fifth Department of Biological Products , Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College , Kunming , Yunnan Province , People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.affiliation: a The Fifth Department of Biological Products , Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College , Kunming , Yunnan Province , People's Republic of China.
authors.forename: Yina
authors.initials: Y
authors.lastname: Cun
authors.affiliation: a The Fifth Department of Biological Products , Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College , Kunming , Yunnan Province , People's Republic of China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Song
authors.affiliation: a The Fifth Department of Biological Products , Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College , Kunming , Yunnan Province , People's Republic of China.
authors.forename: Guoyang
authors.initials: G
authors.lastname: Liao
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002522:Chlorocebus aethiops; D064420:Drug-Related Side Effects and Adverse Reactions / Q000473:pathology; D005260:Female; D005289:Ferrets; D006168:Guinea Pigs; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D009981:Influenza B virus / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000009:adverse effects*; D008807:Mice, Inbred BALB C; D016865:Reassortant Viruses / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D013678:Technology, Pharmaceutical; D014709:Vero Cells
chemical_list: D007252:Influenza Vaccines
keywords: Vero cells; influenza virus; reassortant; safety assessment