title: A Case-Control Study on the Oxidative Balance of 50% Autologous Serum Eye Drops.
abstract: Importance. Autologous serum (AS) eye drops are recommended for severe dry eye in patients with ocular surface disease. No description of the antioxidant balance of AS eye drops has been reported in the literature. Objective. This study sought to evaluate the total reactive antioxidant potential (TRAP) and concentration of reactive oxygen species (ROS) in samples of 50% AS eye drops and their correlations with the demographic characteristics and lifestyle habits of patients with ocular surface disease and healthy controls. Design. This was a case-control study with a 3-month follow-up period. Participants. 16 patients with severe dry eye disease of different etiologies and 17 healthy controls matched by age, gender, and race were included. Results. TRAP and ROS were detected at all evaluated times. There were no differences in the mean ROS (p = 0.429) or TRAP (p = 0.475) levels between cases and controls. No statistically significant differences in the concentrations of ROS or TRAPs were found at 0, 15, or 30 days (p for ROS = 0.087 and p for TRAP = 0.93). Neither the demographic characteristics nor the lifestyle habits were correlated with the oxidative balance of the 50% AS eye drops. Conclusions and Relevance. Both fresh and frozen 50% AS eye drops present antioxidant capacities and ROS in an apparently stable balance. Moreover, patients with ocular surface disease and normal controls produce equivalent AS eye drops in terms of oxidative properties.
authors.affiliation: Faculty of Medicine, Universidade Federal de São Paulo, 04023-062 São Paulo, SP, Brazil; Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil.
authors.forename: Patrícia Ioschpe
authors.identifier: 0000-0001-5503-2331
authors.initials: PI
authors.lastname: Gus
authors.affiliation: Faculty of Medicine, Universidade Federal do Rio Grande do Sul, 90035-190 Porto Alegre, RS, Brazil.
authors.forename: Diane
authors.initials: D
authors.lastname: Marinho
authors.affiliation: Faculty of Medicine, Universidade Federal do Rio Grande do Sul, 90035-190 Porto Alegre, RS, Brazil.
authors.forename: Samira
authors.initials: S
authors.lastname: Zelanis
authors.affiliation: Faculty of Medicine, Universidade Federal do Rio Grande do Sul, 90035-190 Porto Alegre, RS, Brazil.
authors.forename: Adriane
authors.initials: A
authors.lastname: Belló-Klein
authors.affiliation: Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil.
authors.forename: Claudete
authors.initials: C
authors.lastname: Locatelli
authors.affiliation: Faculty of Medicine, Universidade Federal do Rio Grande do Sul, 90035-190 Porto Alegre, RS, Brazil.
authors.forename: Felipe
authors.initials: F
authors.lastname: Nicola
authors.affiliation: Faculty of Medicine, Universidade Federal do Rio Grande do Sul, 90035-190 Porto Alegre, RS, Brazil.
authors.forename: Ana Laura
authors.initials: AL
authors.lastname: Kunzler
authors.affiliation: Laboratory of Cardiovascular Physiology, Universidade Federal do Rio Grande do Sul, 90035-190 Porto Alegre, RS, Brazil.
authors.forename: Tania Regina Gatelli
authors.initials: TR
authors.lastname: Fernandes
authors.affiliation: Faculty of Medicine, Universidade Federal do Rio Grande do Sul, 90035-190 Porto Alegre, RS, Brazil.
authors.forename: Cristina Campos
authors.initials: CC
authors.lastname: Carraro
authors.affiliation: Department of Ophthalmology, Universidade Federal de São Paulo, 04023-062 São Paulo, SP, Brazil.
authors.forename: Luciene
authors.initials: L
authors.lastname: Barbosa
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D016022:Case-Control Studies; D015352:Dry Eye Syndromes / Q000188:drug therapy*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008019:Life Style; D008297:Male; D008875:Middle Aged; D009883:Ophthalmic Solutions / Q000627:therapeutic use*; D018384:Oxidative Stress; D011788:Quality of Life; D017382:Reactive Oxygen Species / Q000378:metabolism; D044967:Serum / Q000737:chemistry*; D014182:Transplantation, Autologous
chemical_list: D000975:Antioxidants; D009883:Ophthalmic Solutions; D017382:Reactive Oxygen Species