title: Novosphingobium pokkalii sp nov, a novel rhizosphere-associated bacterium with plant beneficial properties isolated from saline-tolerant pokkali rice.
abstract: Pokkali rice varieties are known for their saline tolerance when specifically grown in coastal saline affected agri-fields of southern Kerala. These fields are prone to seawater intrusion. During characterization of phytobeneficial rhizobacteria from this pokkali rice, L3E4Twas isolated. This strain showed some plant growth-promoting functions (production of indole acetic acid (IAA), acetoin, and siderophore), biofilm formation and capacity to use a wide range of plant-derived organic compounds. In planta assay under axenic conditions showed a positive effect of L3E4Ton pokkali rice growth; importantly, it was able to attach and colonize pokkali rice roots in the presence of natural seawater, a key adaptation required for survival in pokkali rice fields. Phylogenetic analysis using 16S rRNA, recA, and gyrB gene sequences showed that strain L3E4Tbelongs to the genus Novosphingobium, with Novosphingobium capsulatum GIFU 11526Tand Novosphingobium rhizosphaerae JM.1Tbeing the nearest phylogenetic relatives. In addition, DNA-DNA hybridization analysis and phenotypic traits established that this strain belongs to a novel Novosphingobium species, for which we propose the name Novosphingobium pokkalii sp. nov. The type strain is represented by strain L3E4T(=MTCC 12357T= KCTC 42224T).
authors.affiliation: Biotechnology Department, National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram, 695 019, Kerala, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110 001, India.
authors.forename: Ramya
authors.initials: R
authors.lastname: Krishnan
authors.affiliation: Biotechnology Department, National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram, 695 019, Kerala, India.
authors.forename: Rahul R
authors.initials: RR
authors.lastname: Menon
authors.affiliation: Biotechnology Department, National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram, 695 019, Kerala, India.
authors.lastname: Likhitha
authors.affiliation: Institute of Microbiology, Veterinary University Vienna, A-1210, Vienna, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Tanaka
authors.affiliation: Microbial Type Culture Collection & Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sec-39A, Chandigarh, 160036, India.
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Krishnamurthi
authors.affiliation: Biotechnology Department, National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram, 695 019, Kerala, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110 001, India. Electronic address: rameshkumar@niist.res.in.
authors.forename: N
authors.initials: N
authors.lastname: Rameshkumar
mesh_terms: D000093:Acetoin / Q000378:metabolism; D061245:Axenic Culture; D015373:Bacterial Typing Techniques; D018441:Biofilms; D018556:Crops, Agricultural / Q000382:microbiology*; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000382:microbiology*; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D011938:Rec A Recombinases / Q000235:genetics; D058441:Rhizosphere; D055051:Salt-Tolerant Plants / Q000382:microbiology; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D017262:Siderophores / Q000096:biosynthesis; D042301:Sphingomonadaceae / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D007210:Indoleacetic Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D017262:Siderophores; C030737:indoleacetic acid; D000093:Acetoin; D011938:Rec A Recombinases; D027081:DNA Gyrase
keywords: Novosphingobium; Phytobeneficial; Pokkali rice; Rhizobacteria; Seawater