title: Kinetics of lipid production at lab scale fermenters by a new isolate of Yarrowia lipolytica SKY7.
abstract: The objective of this work was to study the kinetics of lipid production at lab scale fermenters by a new isolate of Yarrowia lipolytica SKY7. The model terms glycerol concentration inoculum and C/N ratio with inoculum were found to be significant for lipid production. Lipid production was found to be higher in glycerol 82.5g/L, C/N ratio 75 and inoculum volume 6.25%. Optimized culture conditions were tested at 15L bench scale reactor. The biomass concentration and lipid content obtained was 29.5g/L and 50% (w/w), respectively. The yield coefficients were calculated and found to be 0.332g/g (g biomass/g of glycerol) of biomass and 0.179g/g (g lipid/g glycerol consumed) for lipid. Observed rates of lipid production show lipid production from 30h of fermentation. Out of the total glycerol consumed, 41.1% glycerol was converted into biomass, lipid, and citric acid.
authors.affiliation: Université du Québec, Institut national de la recherche scientifique, Centre Eau, Terre & Environnement, 490 rue de la Couronne, QC G1K 9A9, Canada.
authors.forename: Kuttiraja
authors.initials: K
authors.lastname: Mathiazhakan
authors.affiliation: Université du Québec, Institut national de la recherche scientifique, Centre Eau, Terre & Environnement, 490 rue de la Couronne, QC G1K 9A9, Canada.
authors.forename: Dhouha
authors.initials: D
authors.lastname: Ayed
authors.affiliation: Université du Québec, Institut national de la recherche scientifique, Centre Eau, Terre & Environnement, 490 rue de la Couronne, QC G1K 9A9, Canada. Electronic address: rd.tyagi@ete.inrs.ca.
authors.forename: Rajeshwar Dayal
authors.initials: RD
authors.lastname: Tyagi
mesh_terms: D056804:Biofuels; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D019343:Citric Acid / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis*; D000432:Methanol / Q000378:metabolism; D025062:Yarrowia / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D056804:Biofuels; D005230:Fatty Acids, Nonesterified; D008055:Lipids; D019343:Citric Acid; D005990:Glycerol; D000432:Methanol
keywords: Biomass; Fermentation; Microbial lipids; RSM optimization; Yarrowia lipolytica