title: Biological application of Compressed Sensing Tomography in the Scanning Electron Microscope.
abstract: The three-dimensional tomographic reconstruction of a biological sample, namely collagen fibrils in human dermal tissue, was obtained from a set of projection-images acquired in the Scanning Electron Microscope. A tailored strategy for the transmission imaging mode was implemented in the microscope and proved effective in acquiring the projections needed for the tomographic reconstruction. Suitable projection alignment and Compressed Sensing formulation were used to overcome the limitations arising from the experimental acquisition strategy and to improve the reconstruction of the sample. The undetermined problem of structure reconstruction from a set of projections, limited in number and angular range, was indeed supported by exploiting the sparsity of the object projected in the electron microscopy images. In particular, the proposed system was able to preserve the reconstruction accuracy even in presence of a significant reduction of experimental projections.
authors.affiliation: Brescia University, Dept. of Information Engineering, Via Branze, 25123 Brescia, Italy.|CNR - National Institute of Optics, Via Valotti, 25123 Brescia, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Ferroni
authors.affiliation: Brescia University, Dept. of Information Engineering, Via Branze, 25123 Brescia, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Signoroni
authors.affiliation: Brescia University, Dept. of Information Engineering, Via Branze, 25123 Brescia, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Sanzogni
authors.affiliation: CNR - Microelectronic and Microsystems Institute, via Gobetti 101, 40129 Bologna, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Masini
authors.affiliation: CNR - Microelectronic and Microsystems Institute, via Gobetti 101, 40129 Bologna, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Migliori
authors.affiliation: CNR - Microelectronic and Microsystems Institute, via Gobetti 101, 40129 Bologna, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Ortolani
authors.affiliation: CNR - Microelectronic and Microsystems Institute, via Gobetti 101, 40129 Bologna, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pezza
authors.affiliation: CNR - Microelectronic and Microsystems Institute, via Gobetti 101, 40129 Bologna, Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Morandi