title: Stable isotope dimethyl labelling for quantitative proteomics and beyond.
abstract: Stable-isotope reductive dimethylation, a cost-effective, simple, robust, reliable and easy-to- multiplex labelling method, is widely applied to quantitative proteomics using liquid chromatography-mass spectrometry. This review focuses on biological applications of stable-isotope dimethyl labelling for a large-scale comparative analysis of protein expression and post-translational modifications based on its unique properties of the labelling chemistry. Some other applications of the labelling method for sample preparation and mass spectrometry-based protein identification and characterization are also summarized.This article is part of the themed issue 'Quantitative mass spectrometry'.
authors.affiliation: Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, Taiwan, Republic of China jlhsu@mail.npust.edu.tw.
authors.forename: Jue-Liang
authors.initials: JL
authors.lastname: Hsu
authors.affiliation: Department of Chemistry, National Cheng Kung University, Tainan City, Taiwan, Republic of China shchen@mail.ncku.edu.tw.
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Chen
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry; D008745:Methylation; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*
keywords: LC-MS/MS; dimethyl labelling; post-translational modifications; quantitative proteomics; stable-isotope labelling