title: Canova medication changes TNF-α and IL-10 serum levels in mice infected with Trypanosoma cruzi Y strain.
abstract: OBJECTIVE
  To identify whether Canova medication changes TNF-α and IL-10 serum levels in mice infected with Trypanosoma cruzi Y strain.
  
  
  METHODS
  Animals were divided into five groups: non-treated infected animals (I); benznidazole-treated infected animals (Bz; 100 mg/kg body weight, single daily dose by gavage); Canova medication (CM) treated infected animals (CM; 0.2 mL/animal, single daily dose by gavage); benznidazole- and Canova medication-treated infected animals with the above-mentioned dose (Bz+CM); and non-infected animals (C). TNF-α and IL-10 levels were determined in serum aliquots after 4, 7, 10, 13, and 29 days of infection. An ELISA technique was employed with R&D System Inc. antibody pairs.
  
  
  RESULTS
  A high increase in TNF-α and IL-10 levels occurred in the infected and CM-treated groups within the treatment employed on the 10th day after infection, coupled with a IL-10 decrease on the 13th day after infection when compared with the other experimental groups.
  
  
  CONCLUSIONS
  CM may change the balance between plasma cytokine levels (TNF-α and IL-10) in mice infected with Y strain T. cruzi, with important consequences leading towards a more severe infection.
authors.affiliation: Laboratory of Parasitology, Department of Basic Sciences of Health, State University of Maringá, Paraná, Brazil.
authors.forename: Áurea Regina Telles
authors.initials: ÁRT
authors.lastname: Pupulin
authors.affiliation: Laboratory of Hepatic Metabolism, Department of Biochemistry, State University of Maringá, Paraná, Brazil.
authors.forename: Lívia
authors.initials: L
authors.lastname: Bracht
authors.affiliation: Laboratory of Immunology, Department of Basic Sciences of Health, State University of Maringá, Paraná, Brazil.
authors.forename: Márcia Machado
authors.initials: MM
authors.lastname: de Oliveira Dalalio
authors.affiliation: Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá, Paraná, Brazil.
authors.forename: Francielli Maria
authors.initials: FM
authors.lastname: de Souza Silva-Comar
authors.affiliation: Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá, Paraná, Brazil.
authors.forename: Bruno Ambrósio
authors.initials: BA
authors.lastname: da Rocha
authors.affiliation: Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá, Paraná, Brazil.
authors.forename: Franciele Queiroz
authors.initials: FQ
authors.lastname: Ames
authors.affiliation: Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá, Paraná, Brazil.
authors.forename: Roberto Kenji Nakamura
authors.initials: RKN
authors.lastname: Cuman
authors.affiliation: Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá, Paraná, Brazil. Electronic address: ciomar.amado@gmail.com.
authors.forename: Ciomar Aparecida
authors.initials: CA
authors.lastname: Bersani-Amado
keywords: Canova medication; Cytokines; Trypanosoma cruzi