title: X-radiation enhances the collagen type I strap formation and migration potentials of colon cancer cells.
abstract: Rectal cancer treatment still fails with local and distant relapses of the disease. It is hypothesized that radiotherapy could stimulate cancer cell dissemination and metastasis. In this study, we evaluated the effect of X-radiation on collagen type I strap formation potential, i.e. matrix remodeling associated with mesenchymal cell migration, and behaviors of SW480, SW620, HCT116 p53+/+ and HCT116 p53-/- colon cancer cells. We determined a radiation-induced increase in collagen type I strap formation and migration potentials of SW480 and HCT116 p53+/+. Further studies with HCT116 p53+/+, indicated that after X-radiation strap forming cells have an increased motility. More, we detected a decrease in adhesion potential and mature integrin β1 expression, but no change in non-muscle myosin II expression for HCT116 p53+/+ after X-radiation. Integrin β1 neutralization resulted in a decreased cell adhesion and collagen type I strap formation in both sham and X-radiated conditions. Our study indicates collagen type I strap formation as a potential mechanism of colon cancer cells with increased migration potential after X-radiation, and suggests that other molecules than integrin β1 and non-muscle myosin II are responsible for the radiation-induced collagen type I strap formation potential of colon cancer cells. This work encourages further molecular investigation of radiation-induced migration to improve rectal cancer treatment outcome.
authors.affiliation: Department of Oncology and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.|Molecular Microscopy, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Blockhuys
authors.affiliation: Department of Oncology and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: Molecular Microscopy, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
authors.forename: Nisha Rani
authors.initials: NR
authors.lastname: Agarwal
authors.affiliation: Molecular Microscopy, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
authors.forename: Annika
authors.initials: A
authors.lastname: Enejder
authors.affiliation: Medical Microbiology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Vesa
authors.initials: V
authors.lastname: Loitto
authors.affiliation: Department of Oncology and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Sun
mesh_terms: D024722:Cardiac Myosins / Q000032:analysis; D002448:Cell Adhesion / Q000528:radiation effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000528:radiation effects; D024042:Collagen Type I / Q000737:chemistry*; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D019012:Integrin beta1 / Q000502:physiology; D020409:Molecular Motor Proteins / Q000032:analysis / Q000502:physiology; D018995:Myosin Heavy Chains / Q000032:analysis / Q000502:physiology; D018994:Myosin Light Chains / Q000032:analysis; D014965:X-Rays
chemical_list: D024042:Collagen Type I; D019012:Integrin beta1; C103814:MYH9 protein, human; D020409:Molecular Motor Proteins; D018994:Myosin Light Chains; C107184:myosin light chain 2; D024722:Cardiac Myosins; D018995:Myosin Heavy Chains
keywords: X-radiation; cell migration; collagen type 1; colorectal cancer; integrin β1