title: Histopathological effects following short-term coexposure of Cyprinus carpio to nanoparticles of TiO2 and CuO.
abstract: The aim of this research was to investigate the coexposure of nanoparticles of titanium dioxide (TiO2) and copper oxide (CuO) on the alterations of the gill, intestine, kidney, and liver tissues of carps (Cyprinus carpio). In this study, carps (length 23 ± 1.5 cm; weight 13 ± 1.3 g) were divided into six groups of 15 each and exposed to 2.5 and 5.0 mg L(-1) of CuO nanoparticles (NPs), 10.0 mg L(-1) of TiO2 NPs, and 2.5 and 5.0 mg L(-1) of CuO NPs + 10.0 mg L(-1) of TiO2 NP mixture. Fish were sampled for histopathological studies after hematoxylin-eosin staining. Results indicated that the more kinds of histopathology anomalies observed with CuO NP and TiO2 NP mixture were broadly of the same type as CuO NPs and TiO2 NPs alone, but the severity or incidence of injuries of gill, intestine, liver, and kidney of carps in the mixture of CuO NPs + TiO2 NPs was higher than that of each NP alone. Moreover, behavioral changes in carps exposed to CuO NP and TiO2 NP mixture such as hyperactivity, loss of balance, and convulsions were higher than those to CuO NPs and TiO2 NPs alone. In conclusion, the presence of TiO2 NPs enhanced the effects of NPs of copper oxide in terms of histopathological changes in carps.
authors.affiliation: Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.|Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: Borhan
authors.initials: B
authors.lastname: Mansouri
authors.affiliation: Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: Afshin
authors.initials: A
authors.lastname: Maleki
authors.affiliation: Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran. davaribehroz@gmail.com.|Department of Entomology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. davaribehroz@gmail.com.
authors.forename: Behroz
authors.initials: B
authors.lastname: Davari
authors.affiliation: Department of Fisheries, Faculty of Natural Resources, University of Kurdistan, Sanandaj, Iran.
authors.forename: Seyed Ali
authors.initials: SA
authors.lastname: Johari
authors.affiliation: Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Shahmoradi
authors.affiliation: Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Mohammadi
authors.affiliation: Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: Siros
authors.initials: S
authors.lastname: Shahsavari
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002347:Carps; D003300:Copper / Q000633:toxicity*; D004357:Drug Synergism; D005880:Gills / Q000187:drug effects / Q000473:pathology; D007422:Intestines / Q000187:drug effects / Q000473:pathology; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D053758:Nanoparticles / Q000633:toxicity*; D014025:Titanium / Q000633:toxicity*
chemical_list: C009495:titanium dioxide; D003300:Copper; D014025:Titanium; C000520:cuprous oxide
keywords: Acute toxicity; Coexposure; CuO; Histopathology; Nanoparticles; TiO2