title: Oocytes from small and large follicles exhibit similar development competence following goat cloning despite their differences in meiotic and cytoplasmic maturation.
abstract: Reduced developmental competence after IVF has been reported using oocyte derived from small follicles in several species including cattle, sheep, and goats. No information is currently available about the effect of follicle size of the cytoplast donor on in vivo development after somatic cell nuclear transfer (SCNT) in goats. Oocytes collected from large (≥3 mm) and small follicles (<3 mm) were examined for maturation and in vivo developmental competence after SCNT. Significantly greater maturation rate was observed in oocytes derived from large follicles compared with that of small follicles (51.6% and 33.7%, P < 0.05). Greater percent of large follicle oocytes exhibited a low glucose-6-phosphate dehydrogenase activity at germinal vesicle stage compared with small follicle oocytes (54.9% and 38.7%, P < 0.05). Relative mRNA expression analysis of 48 genes associated with embryonic and fetal development revealed that three genes (MATER, IGF2R, and GRB10) had higher level of expression in metaphase II oocytes from large follicles compared with oocytes from small follicles. Nevertheless, no difference was observed in pregnancy rates (33.3% vs. 47.1%) and birth rates (22.2% vs. 16.7%) after SCNT between the large and small follicle groups). These results indicate that metaphase II cytoplasts from small and large follicles have similar developmental competence when used in goat SCNT.
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Hall
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA.
authors.forename: Misha
authors.initials: M
authors.lastname: Regouski
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA.
authors.forename: Qinggang
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA; School of Veterinary Medicine, Utah State University, Logan, Utah, USA.
authors.forename: Heloisa M
authors.initials: HM
authors.lastname: Rutigliano
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA; School of Veterinary Medicine, Utah State University, Logan, Utah, USA.
authors.forename: Rusty
authors.initials: R
authors.lastname: Stott
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA; School of Veterinary Medicine, Utah State University, Logan, Utah, USA.
authors.forename: Kerry A
authors.initials: KA
authors.lastname: Rood
authors.affiliation: USDA ARS Poisonous Plant Research Laboratory, Logan, Utah, USA.
authors.forename: Kip E
authors.initials: KE
authors.lastname: Panter
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA. Electronic address: irina.polejaeva@usu.edu.
authors.forename: Irina A
authors.initials: IA
authors.lastname: Polejaeva
mesh_terms: D000818:Animals; D019976:Cloning, Organism / Q000662:veterinary*; D003593:Cytoplasm / Q000502:physiology*; D004789:Enzyme Activation; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006041:Goats; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary*; D008540:Meiosis / Q000502:physiology*; D008677:Metaphase; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000502:physiology*
chemical_list: D005954:Glucosephosphate Dehydrogenase
keywords: Brilliant cresyl blue; Goat somatic cell nuclear transfer; Oocyte activation; Oocyte gene expression; Real-time PCR