title: Nitrogen and phosphorus removal coupled with carbohydrate production by five microalgae cultures cultivated in biogas slurry.
abstract: In this study, five microalgae strains were cultured for their ability to survive in biogas slurry, remove nitrogen resources and accumulate carbohydrates. It was proved that five microalgae strains adapted in biogas slurry well without ammonia inhibition. Among them, Chlorella vulgaris ESP-6 showed the best performance on carbohydrate accumulation, giving the highest carbohydrate content of 61.5% in biogas slurry and the highest ammonia removal efficiency and rate of 96.3% and 91.7mg/L/d respectively in biogas slurry with phosphorus and magnesium added. Additionally, the absence of phosphorus and magnesium that can be adverse for biomass accumulation resulted in earlier timing of carbohydrate accumulation and magnesium was firstly recognized and proved as the influence factor for carbohydrate accumulation. Microalgae that cultured in biogas slurry accumulated more carbohydrate in cell, making biogas slurry more suitable medium for the improvement of carbohydrate content, thus can be regarded as a new strategy to accumulate carbohydrate.
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, PR China.
authors.forename: Fen
authors.initials: F
authors.lastname: Tan
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, PR China.
authors.forename: Siyu
authors.initials: S
authors.lastname: Zhouyang
authors.affiliation: College of the Environment and Ecology, Xiamen University, Xiamen 361005, PR China.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, PR China.
authors.forename: Youping
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yuanpeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, PR China; College of the Environment and Ecology, Xiamen University, Xiamen 361005, PR China. Electronic address: kelqb@xmu.edu.cn.
authors.forename: Qingbiao
authors.initials: Q
authors.lastname: Li
mesh_terms: D000641:Ammonia / Q000737:chemistry; D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D002241:Carbohydrates / Q000737:chemistry*; D048408:Chlorella vulgaris; D058086:Microalgae; D009584:Nitrogen / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D010758:Phosphorus; D000641:Ammonia; D009584:Nitrogen
keywords: Ammonia nitrogen uptake; Biogas slurry; Carbohydrate accumulation; Chlorella vulgaris