title: The Regulatory Effects of Long Noncoding RNA-ANCR on Dental Tissue-Derived Stem Cells.
abstract: Long noncoding RNAs (lncRNA) have been recognized as important regulators in diverse biological processes, such as transcriptional regulation, stem cell proliferation, and differentiation. Previous study has demonstrated that lncRNA-ANCR (antidifferentiation ncRNA) plays a key role in regulating the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). However, little is known about the role of ANCR in regulating other types of dental tissue-derived stem cells (DTSCs) behaviours (including proliferation and multiple-potential of differentiation). In this study, we investigated the regulatory effects of lncRNA-ANCR on the proliferation and differentiation (including osteogenic, adipogenic, and neurogenic differentiation) of DTSCs, including dental pulp stem cells (DPSCs), PDLSCs, and stem cells from the apical papilla (SCAP) by downregulation of lncRNA-ANCR. We found that downregulation of ANCR exerted little effect on proliferation of DPSCs and SCAP but promoted the osteogenic, adipogenic, and neurogenic differentiation of DTSCs. These data provide an insight into the regulatory effects of long noncoding RNA-ANCR on DTSCs and indicate that ANCR is a very important regulatory factor in stem cell differentiation.
authors.affiliation: Institution of Stomatology, The PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Jia
authors.affiliation: Department of Endodontics, Xiamen Stomatology Hospital, Teaching Hospital of Fujian Medical University, No. 1309 Lvling Road, Xiamen, Fujian 361003, China; State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, No. 145 Western Changle Road, Xi'an, Shaanxi 710032, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, No. 145 Western Changle Road, Xi'an, Shaanxi 710032, China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, No. 145 Western Changle Road, Xi'an, Shaanxi 710032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Institution of Stomatology, The PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, China.
authors.forename: Bin
authors.identifier: 0000-0001-5278-0182
authors.initials: B
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Operative Dentistry & Endodontics, School of Stomatology, Fourth Military Medical University, No. 145 Western Changle Road, Xi'an, Shaanxi 710032, China.
authors.forename: Longxing
authors.identifier: 0000-0002-8880-5449
authors.initials: L
authors.lastname: Ni