title: A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease.
abstract: Currently there is a lack in fundamental understanding of disease progression of most neurodegenerative diseases, and, therefore, treatments and preventative measures are limited. Consequently, there is a great need for adaptable, yet robust model systems to both investigate elementary disease mechanisms and discover effective therapeutics. We have generated a Tol2 Gateway-compatible toolbox to study neurodegenerative disorders in zebrafish, which includes promoters for astrocytes, microglia and motor neurons, multiple fluorophores, and compatibility for the introduction of genes of interest or disease-linked genes. This toolbox will advance the rapid and flexible generation of zebrafish models to discover the biology of the nervous system and the disease processes that lead to neurodegeneration.
authors.affiliation: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .
authors.forename: Emily K
authors.initials: EK
authors.lastname: Don
authors.affiliation: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .
authors.forename: Isabel
authors.initials: I
authors.lastname: Formella
authors.affiliation: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Badrock
authors.affiliation: 2 Institute for Molecular Bioscience, University of Queensland , Brisbane, Australia .
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Hall
authors.affiliation: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .
authors.forename: Marco
authors.initials: M
authors.lastname: Morsch
authors.affiliation: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .
authors.forename: Elinor
authors.initials: E
authors.lastname: Hortle
authors.affiliation: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .
authors.forename: Alison
authors.initials: A
authors.lastname: Hogan
authors.affiliation: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .
authors.forename: Sharron
authors.initials: S
authors.lastname: Chow
authors.affiliation: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .
authors.forename: Serene S L
authors.initials: SS
authors.lastname: Gwee
authors.affiliation: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .
authors.forename: Jack J
authors.initials: JJ
authors.lastname: Stoddart
authors.affiliation: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .|3 ANZAC Research Institute, Concord Hospital, University of Sydney , Sydney, Australia .
authors.forename: Garth
authors.initials: G
authors.lastname: Nicholson
authors.affiliation: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .
authors.forename: Roger
authors.initials: R
authors.lastname: Chung
authors.affiliation: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University , Sydney, Australia .
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Cole
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D004251:DNA Transposable Elements; D004274:DNA, Recombinant / Q000235:genetics; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D009422:Nervous System Diseases / Q000235:genetics*; D019636:Neurodegenerative Diseases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D049452:Green Fluorescent Proteins
keywords: Tol2 gateway; disease model; neurodegenerative disease; transgenesis