title: Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification.
abstract: Thirty-four microsatellite loci were isolated from three reef fish species; golden snapper Lutjanus johnii, blackspotted croaker Protonibea diacanthus and grass emperor Lethrinus laticaudis using a next generation sequencing approach. Both IonTorrent single reads and Illumina MiSeq paired-end reads were used, with the latter demonstrating a higher quality of reads than the IonTorrent. From the 1-1.5 million raw reads per species, we successfully obtained 10-13 polymorphic loci for each species, which satisfied stringent design criteria. We developed multiplex panels for the amplification of the golden snapper and the blackspotted croaker loci, as well as post-amplification pooling panels for the grass emperor loci. The microsatellites characterized in this work were tested across three locations of northern Australia. The microsatellites we developed can detect population differentiation across northern Australia and may be used for genetic structure studies and stock identification.
authors.affiliation: Research Institute for the Environment and Livelihoods, Charles Darwin University, Darwin, Northern Territory, Australia; North Australia Marine Research Alliance, Arafura Timor Research Facility, Darwin, Northern Territory, Australia.
authors.forename: Laura
authors.initials: L
authors.lastname: Taillebois
authors.affiliation: Molecular Fisheries Laboratory, School of Biomedical Sciences, University of Queensland , Brisbane , Queensland , Australia.
authors.forename: Christine
authors.initials: C
authors.lastname: Dudgeon
authors.affiliation: Molecular Fisheries Laboratory, School of Biomedical Sciences, University of Queensland , Brisbane , Queensland , Australia.
authors.forename: Safia
authors.initials: S
authors.lastname: Maher
authors.affiliation: Research Institute for the Environment and Livelihoods, Charles Darwin University , Darwin , Northern Territory , Australia.
authors.forename: David A
authors.initials: DA
authors.lastname: Crook
authors.affiliation: Fisheries Research, Northern Territory Department of Primary Industries and Fisheries , Berrimah , Northern Territory , Australia.
authors.forename: Thor M
authors.initials: TM
authors.lastname: Saunders
authors.affiliation: Research Institute for the Environment and Livelihoods, Charles Darwin University, Darwin, Northern Territory, Australia; Fisheries Research, Northern Territory Department of Primary Industries and Fisheries, Berrimah, Northern Territory, Australia.
authors.forename: Diane P
authors.initials: DP
authors.lastname: Barton
authors.affiliation: Fisheries Research, Northern Territory Department of Primary Industries and Fisheries , Berrimah , Northern Territory , Australia.
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Taylor
authors.affiliation: C2O Fisheries , Cairns , Queensland , Australia.
authors.forename: David J
authors.initials: DJ
authors.lastname: Welch
authors.affiliation: Western Australian Fisheries and Marine Research Laboratories, Department of Fisheries, Government of Western Australia , North Beach , Western Australia , Australia.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Newman
authors.affiliation: Western Australian Fisheries and Marine Research Laboratories, Department of Fisheries, Government of Western Australia , North Beach , Western Australia , Australia.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Travers
authors.affiliation: Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Douglas, Queensland, Australia; Animal Science, Queensland Department of Agriculture and Fisheries, Brisbane, Queensland, Australia.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Saunders
authors.affiliation: Molecular Fisheries Laboratory, School of Biomedical Sciences, University of Queensland , Brisbane , Queensland , Australia.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Ovenden
keywords: Lethrinus laticaudis; Lutjanus johnii; Microsatellite markers; Multiplex panels; Next-generation sequencing; Protonibea diacanthus