title: Olfactory ensheathing glia cell therapy and tubular conduit enhance nerve regeneration after mouse sciatic nerve transection.
abstract: The regenerative potential of the peripheral nervous system (PNS) is widely known, but functional recovery, particularly in humans, is seldom complete. Therefore, it is necessary to resort to strategies that induce or potentiate the PNS regeneration. Our main objective was to test the effectiveness of Olfactory Ensheathing Cells (OEC) transplantation into a biodegradable conduit as a therapeutic strategy to improve the repair outcome after nerve injury. Sciatic nerve transection was performed in C57BL/6 mice; proximal and distal stumps of the nerve were sutured into the collagen conduit. Two groups were analyzed: DMEM (acellular grafts) and OEC (1×105/2μL). Locomotor function was assessed weekly by Sciatic Function Index (SFI) and Global Mobility Test (GMT). After eight weeks the sciatic nerve was dissected for morphological analysis. Our results showed that the OEC group exhibited many clusters of regenerated nerve fibers, a higher number of myelinated fibers and myelin area compared to DMEM group. The G-ratio analysis of the OEC group showed significantly more fibers on the most suitable sciatic nerve G-ratio index. Motor recovery was accelerated in the OEC group. These data provide evidence that the OEC therapy can improve sciatic nerve functional and morphological recovery and can be potentially translated to the clinical setting.
authors.affiliation: Laboratório de Neurodegeneração e Reparo - Departamento de Patologia - Faculdade de Medicina - Hospital Universitário Clementino Fraga Filho - UFRJ, Rio de Janeir, RJ, Brazil.
authors.forename: Camila Oliveira
authors.initials: CO
authors.lastname: Goulart
authors.affiliation: Laboratório de Neurodegeneração e Reparo - Departamento de Patologia - Faculdade de Medicina - Hospital Universitário Clementino Fraga Filho - UFRJ, Rio de Janeir, RJ, Brazil.
authors.forename: Daniella de Freitas Pereira
authors.initials: DF
authors.lastname: Ângelo Durço
authors.affiliation: Lab. de Neurobiologia Comparativa e do Desenvolvimento - Instituto de Biofísica Carlos Chagas Filho - UFRJ, Rio de Janeiro, Brazil.
authors.forename: Lítia Alves
authors.initials: LA
authors.lastname: de Carvalho
authors.affiliation: Laboratório de Neurodegeneração e Reparo - Departamento de Patologia - Faculdade de Medicina - Hospital Universitário Clementino Fraga Filho - UFRJ, Rio de Janeir, RJ, Brazil.
authors.forename: Júlia Teixeira
authors.initials: JT
authors.lastname: Oliveira
authors.affiliation: Lab. de Neurobiologia Comparativa e do Desenvolvimento - Instituto de Biofísica Carlos Chagas Filho - UFRJ, Rio de Janeiro, Brazil.
authors.forename: Lucinéia
authors.initials: L
authors.lastname: Alves
authors.affiliation: Lab. de Neurobiologia Comparativa e do Desenvolvimento - Instituto de Biofísica Carlos Chagas Filho - UFRJ, Rio de Janeiro, Brazil. Electronic address: leny@biof.ufrj.br.
authors.forename: Leny A
authors.initials: LA
authors.lastname: Cavalcante
authors.affiliation: Laboratório de Neurodegeneração e Reparo - Departamento de Patologia - Faculdade de Medicina - Hospital Universitário Clementino Fraga Filho - UFRJ, Rio de Janeir, RJ, Brazil; Lab. de Neurobiologia Comparativa e do Desenvolvimento - Instituto de Biofísica Carlos Chagas Filho - UFRJ, Rio de Janeiro, Brazil. Electronic address: anamartinez@hucff.ufrj.br.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Blanco Martinez
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D009186:Myelin Sheath / Q000502:physiology / Q000637:transplantation*; D009412:Nerve Fibers / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D009457:Neuroglia / Q000502:physiology*; D066194:Olfactory Cortex; D020127:Recovery of Function / Q000502:physiology; D012583:Schwann Cells / Q000637:transplantation; D012584:Sciatic Nerve / Q000293:injuries
keywords: Cell therapy; Ensheathing cells; Nerve conduit; Nerve regeneration; Olfactory; Peripheral nerve trauma