title: Transcription factor ANAC032 modulates JA/SA signalling in response to Pseudomonas syringae infection.
abstract: Responses to pathogens, including host transcriptional reprogramming, require partially antagonistic signalling pathways dependent on the phytohormones salicylic (SA) and jasmonic (JA) acids. However, upstream factors modulating the interplay of these pathways are not well characterized. Here, we identify the transcription factor ANAC032 from Arabidopsis thaliana as one such regulator in response to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pst). ANAC032 directly represses MYC2 activation upon Pst attack, resulting in blockage of coronatine-mediated stomatal reopening which restricts entry of bacteria into plant tissue. Furthermore, ANAC032 activates SA signalling by repressing NIMIN1, a key negative regulator of SA-dependent defence. Finally, ANAC032 reduces expression of JA-responsive genes, including PDF1.2A Thus, ANAC032 enhances resistance to Pst by generating an orchestrated transcriptional output towards key SA- and JA-signalling genes coordinated through direct binding of ANAC032 to the MYC2, NIMIN1 and PDF1.2A promoters.
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, Potsdam-Golm, Germany.|Max-Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
authors.forename: Annapurna Devi
authors.initials: AD
authors.lastname: Allu
authors.affiliation: Max-Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
authors.forename: Yariv
authors.initials: Y
authors.lastname: Brotman
authors.affiliation: CSIRO Agriculture Flagship, St. Lucia, QLD, Australia.
authors.forename: Gang-Ping
authors.initials: GP
authors.lastname: Xue
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, Potsdam-Golm, Germany balazadeh@mpimp-golm.mpg.de.|Max-Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
authors.forename: Salma
authors.identifier: 0000-0002-5789-4071
authors.initials: S
authors.lastname: Balazadeh
mesh_terms: D017360:Arabidopsis / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D023082:Defensins / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions; D054883:Oxylipins / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D011485:Protein Binding; D044224:Pseudomonas syringae / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D020156:Salicylic Acid / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors
chemical_list: D029681:Arabidopsis Proteins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D002352:Carrier Proteins; D003517:Cyclopentanes; D023082:Defensins; D054883:Oxylipins; D020156:Salicylic Acid; D015534:Trans-Activators; D014157:Transcription Factors; C000616075:ANAC032 protein, Arabidopsis; C473611:MYC2 protein, Arabidopsis; C507154:NIMIN1 protein, Arabidopsis; C011006:jasmonic acid; C487542:At5g44420 protein, Arabidopsis
keywords: 
  Arabidopsis
  ; jasmonic acid; pathogens; salicylic acid; transcription factor