title: Melodinine V, an antitumor bisindole alkaloid with selective cytotoxicity from Melodinus henryi.
abstract: Melodinine V (1) with a vincanol-eburenine skeleton, was isolated from Melodinus henryi. The structure was elucidated by extensive spectroscopic methods and further confirmed by the single crystal X-ray diffraction analysis. Melodinine V showed selective cytotoxic activities against human colon cancer cell line HT-29 and inhibited cell proliferation in a concentration-dependent manner. It induced cell cycle arrest at G1 phase and cellular apoptosis by increasing histone-associated DNA fragmentation in the treated HT-29 cells.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming 650201, PR China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Liu
authors.affiliation: Institute of Chinese Medicine and State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
authors.forename: Grace Gar-Lee
authors.initials: GG
authors.lastname: Yue
authors.affiliation: Institute of Chinese Medicine and State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
authors.forename: Julia Kin-Ming
authors.initials: JK
authors.lastname: Lee
authors.affiliation: College of Pharmaceutical Sciences, South-central University For Nationalities, Wuhan, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming 650201, PR China.
authors.forename: Yun-Li
authors.initials: YL
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming 650201, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Chinese Medicine and State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong. Electronic address: claralau@cuhk.edu.hk.
authors.forename: Clara Bik-San
authors.initials: CB
authors.lastname: Lau
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming 650201, PR China. Electronic address: xdluo@mail.kib.ac.cn.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Luo
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D029062:Apocynaceae / Q000737:chemistry*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D049109:Cell Proliferation / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D046948:Secologanin Tryptamine Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; D046948:Secologanin Tryptamine Alkaloids; C000621369:melodinine V
keywords: Bisindole; Cell cycle arrest; Cellular apoptosis; HT-29 cells; Inhibited cell proliferation; Melodinine V