title: Testing for the erythropoiesis-stimulating agent Sotatercept/ACE-011 (ActRIIA-Fc) in serum by means of Western blotting and LC-HRMS.
abstract: Sotatercept (formerly ACE-011) is a glycosylated, dimeric fusion protein composed of the extracellular domain of the human activin receptor type IIA (ActRIIA) and the Fc region of human IgG1. The protein-based drug candidate acts as a ligand trap which competitively binds to activin A and other members of the transforming growth factor beta superfamily, thus blocking signalling through ActRIIA. Since the inhibition of activin A was found to significantly increase bone formation and quality, Sotatercept was originally developed for the treatment of diseases involving bone loss. But as the protein therapeutic also stimulates erythropoiesis by a mechanism independent of the EPO receptor, it has been evaluated for the treatment of anaemia in rare blood diseases such as beta thalassemia. Due to its positive effects on erythropoiesis and bone formation, Sotatercept may also be misused as performance-enhancing agent in sports. Within this study, two complementary detection assays for Sotatercept and related ActRIIA-Fc fusion proteins in serum samples were developed. While the first assay combines affinity purification and Western blotting to generically detect ActRIIA-Fc fusion proteins irrespective of their amino acid sequence, the liquid chromatography-high resolution mass spectrometry (LC-HRMS) method is highly specific for proteolytic peptides originating from the receptor and Fc domain of Sotatercept. Both approaches can readily be modified to include other pharmaceutical proteins such as therapeutic antibodies, and serve as proof-of-concept for the capability of the approach to detect TGF-β inhibitors and Fc fusion proteins in doping control serum samples. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Center for Preventive Doping Research/Institute of Biochemistry, German Sport University Cologne, Cologne, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Walpurgis
authors.affiliation: Center for Preventive Doping Research/Institute of Biochemistry, German Sport University Cologne, Cologne, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Thomas
authors.affiliation: Center for Preventive Doping Research/Institute of Biochemistry, German Sport University Cologne, Cologne, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Vogel
authors.affiliation: Doping Control Laboratory, AIT Seibersdorf Laboratories, Seibersdorf, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Reichel
authors.affiliation: Center for Preventive Doping Research/Institute of Biochemistry, German Sport University Cologne, Cologne, Germany.
authors.forename: Hans
authors.initials: H
authors.lastname: Geyer
authors.affiliation: Center for Preventive Doping Research/Institute of Biochemistry, German Sport University Cologne, Cologne, Germany.
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Schänzer
authors.affiliation: Center for Preventive Doping Research/Institute of Biochemistry, German Sport University Cologne, Cologne, Germany.|Doping Control Laboratory, AIT Seibersdorf Laboratories, Seibersdorf, Austria.
authors.forename: Mario
authors.initials: M
authors.lastname: Thevis
mesh_terms: D030301:Activin Receptors, Type II / Q000737:chemistry*; D015153:Blotting, Western / Q000379:methods*; D004300:Doping in Sports; D004920:Erythropoiesis / Q000187:drug effects*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000737:chemistry
chemical_list: D030301:Activin Receptors, Type II; D011993:Recombinant Fusion Proteins; D016212:Transforming Growth Factor beta; C542017:sotatercept; C425585:activin receptor type II-A
keywords: ActRIIA-Fc; LC-HRMS; Western blotting; affinity purification; doping