title: In Vivo Models to Address the Function of Polycomb Group Proteins.
abstract: Initially discovered as repressors of homeotic gene expression in Drosophila, Polycomb group (PcG) proteins have now been shown to be involved in a plethora of biological processes. Indeed, by repressing a large number of target genes, including specific lineage genes, these chromatin factors play major roles in a multitude of cellular functions, such as pluripotency, differentiation, reprogramming, tissue regeneration, and nuclear organization. In this book chapter are presented in vivo approaches and technologies, which have been used in both mammalian and Drosophila systems to study the cellular functions of Polycomb group proteins.
authors.affiliation: Institut de Génétique Humaine, CNRS UPR 1142, 141, rue de la Cardonille, 34396, Montpellier, France. frederic.bantignies@igh.cnrs.fr.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bantignies
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002843:Chromatin / Q000235:genetics / Q000302:isolation & purification*; D004330:Drosophila; D008322:Mammals; D008967:Molecular Biology / Q000379:methods*; D063146:Polycomb-Group Proteins / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D002843:Chromatin; D063146:Polycomb-Group Proteins
keywords: Differentiation; Heterokaryons; Myogenesis; Nuclear organization; Polycomb; Reprogramming; TALEN technology; Transdetermination