title: Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii.
abstract: Photosynthetic organisms are exposed to drastic changes in light conditions, which can affect their photosynthetic efficiency and induce photodamage. To face these changes, they have developed a series of acclimation mechanisms. In this work, we have studied the acclimation strategies of Chlamydomonas reinhardtii, a model green alga that can grow using various carbon sources and is thus an excellent system in which to study photosynthesis. Like other photosynthetic algae, it has evolved inducible mechanisms to adapt to conditions where carbon supply is limiting. We have analyzed how the carbon availability influences the composition and organization of the photosynthetic apparatus and the capacity of the cells to acclimate to different light conditions. Using electron microscopy, biochemical, and fluorescence measurements, we show that differences in CO2availability not only have a strong effect on the induction of the carbon-concentrating mechanisms but also change the acclimation strategy of the cells to light. For example, while cells in limiting CO2maintain a large antenna even in high light and switch on energy-dissipative mechanisms, cells in high CO2reduce the amount of pigments per cell and the antenna size. Our results show the high plasticity of the photosynthetic apparatus of C. reinhardtii This alga is able to use various photoacclimation strategies, and the choice of which to activate strongly depends on the carbon availability.
authors.affiliation: Biophysics of Photosynthesis, Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (I.P., R.F., E.D., R.C.); and.|Genetics and Physiology of Microalgae, Institut de Botanique, Université de Liège, 4000 Liege, Belgium (P.C.).
authors.forename: Iryna
authors.identifier: 0000-0002-7748-3173
authors.initials: I
authors.lastname: Polukhina
authors.affiliation: Biophysics of Photosynthesis, Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (I.P., R.F., E.D., R.C.); and.|Genetics and Physiology of Microalgae, Institut de Botanique, Université de Liège, 4000 Liege, Belgium (P.C.).
authors.forename: Rikard
authors.initials: R
authors.lastname: Fristedt
authors.affiliation: Biophysics of Photosynthesis, Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (I.P., R.F., E.D., R.C.); and.|Genetics and Physiology of Microalgae, Institut de Botanique, Université de Liège, 4000 Liege, Belgium (P.C.).
authors.forename: Emine
authors.initials: E
authors.lastname: Dinc
authors.affiliation: Biophysics of Photosynthesis, Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (I.P., R.F., E.D., R.C.); and.|Genetics and Physiology of Microalgae, Institut de Botanique, Université de Liège, 4000 Liege, Belgium (P.C.).
authors.forename: Pierre
authors.identifier: 0000-0001-9799-0546
authors.initials: P
authors.lastname: Cardol
authors.affiliation: Biophysics of Photosynthesis, Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (I.P., R.F., E.D., R.C.); and r.croce@vu.nl.|Genetics and Physiology of Microalgae, Institut de Botanique, Université de Liège, 4000 Liege, Belgium (P.C.) r.croce@vu.nl.
authors.forename: Roberta
authors.identifier: 0000-0003-3469-834X
authors.initials: R
authors.lastname: Croce
mesh_terms: D000064:Acclimatization / Q000187:drug effects / Q000528:radiation effects*; D020418:Algal Proteins / Q000378:metabolism; D002244:Carbon / Q000494:pharmacology*; D002338:Carotenoids / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects* / Q000648:ultrastructure; D008027:Light; D008954:Models, Biological; D045331:Photosystem I Protein Complex / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism; D010860:Pigments, Biological / Q000378:metabolism; D020524:Thylakoids / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects
chemical_list: D020418:Algal Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010860:Pigments, Biological; D002338:Carotenoids; D002244:Carbon