title: Omega-3 fatty acids reduce lipopolysaccharide-induced abnormalities in expression of connexin-40 in aorta of hereditary hypertriglyceridemic rats.
abstract: Omega-3 fatty acids (omega3FA) are known to reduce hypertriglyceridemia- and inflammation-induced vascular wall diseases. However, mechanisms of their effects are not completely clear. We examined, whether 10-day omega3FA diet can reduce bacterial lipopolysaccharide-induced changes in expression of gap junction protein connexin40 (Cx40) in the aorta of hereditary hypertriglyceridemic (hHTG) rats. After administration of a single dose of lipopolysaccharide (LPS, 1 mg/kg, i.p.) to adult hHTG rats, animals were fed with omega3FA diet (30 mg/kg/day) for 10 days. LPS decreased Cx40 expression that was associated with reduced acetylcholine-induced relaxation of aorta. Omega3FA administration to LPS rats had partial anti-inflammatory effects, associated with increased Cx40 expression and improved endothelium dependent relaxation of the aorta. Our results suggest that 10-day omega3FA diet could protect endothelium-dependent relaxation of the aorta of hHTG rats against LPS-induced damage through the modulation of endothelial Cx40 expression.
authors.affiliation: Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic. usrdfrim@savba.sk.
authors.forename: K
authors.initials: K
authors.lastname: Frimmel
authors.forename: R
authors.initials: R
authors.lastname: Sotníková
authors.forename: J
authors.initials: J
authors.lastname: Navarová
authors.forename: I
authors.initials: I
authors.lastname: Bernátová
authors.forename: J
authors.initials: J
authors.lastname: KriŽák
authors.forename: Z
authors.initials: Z
authors.lastname: Haviarová
authors.forename: B
authors.initials: B
authors.lastname: Kura
authors.forename: J
authors.initials: J
authors.lastname: Slezák
authors.forename: Ľ
authors.initials: Ľ
authors.lastname: Okruhlicová
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism; D015153:Blotting, Western; D017630:Connexins / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology / Q000627:therapeutic use*; D015228:Hypertriglyceridemia / Q000151:congenital / Q000178:diet therapy* / Q000378:metabolism; D008070:Lipopolysaccharides; D008297:Male; D051381:Rats; D000097003:Gap Junction alpha-5 Protein; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D017630:Connexins; D015525:Fatty Acids, Omega-3; D008070:Lipopolysaccharides; D000097003:Gap Junction alpha-5 Protein; C539355:CD68 protein, rat; D000097382:CD68 Molecule