title: Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform.
abstract: Automation of cell culture would facilitate stable cell expansion with consistent quality. In the present study, feasibility of an automated closed-cell culture system "P 4C S" for an embryoid body- (EB-) explant outgrowth culture was investigated as a model case for explant culture. After placing the induced pluripotent stem cell- (iPSC-) derived EBs into the system, the EBs successfully adhered to the culture surface and the cell outgrowth was clearly observed surrounding the adherent EBs. After confirming the outgrowth, we carried out subculture manipulation, in which the detached cells were simply dispersed by shaking the culture flask, leading to uniform cell distribution. This enabled continuous stable cell expansion, resulting in a cell yield of 3.1 × 10(7). There was no evidence of bacterial contamination throughout the cell culture experiments. We herewith developed the automated cultivation platform for EB-explant outgrowth cells.
authors.affiliation: Department of Reproductive Biology, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan; Medical Devices Division, Kaneka Corporation, Osaka 530-8288, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tone
authors.affiliation: Department of Reproductive Biology, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan; Laboratory for Medical Engineering, Division of Materials and Chemical Engineering, Graduate School of Engineering, Yokohama National University, Kanagawa 240-8501, Japan.
authors.forename: Saeko
authors.initials: S
authors.lastname: Yoshioka
authors.affiliation: Medical Device Development Laboratories, Kaneka Corporation, Hyōgo 676-8688, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Akiyama
authors.affiliation: Medical Device Development Laboratories, Kaneka Corporation, Hyōgo 676-8688, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Nishimura
authors.affiliation: Medical Device Development Laboratories, Kaneka Corporation, Hyōgo 676-8688, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ichimura
authors.affiliation: Medical Device Development Laboratories, Kaneka Corporation, Hyōgo 676-8688, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Nakatani
authors.affiliation: Division of Carcinogenesis and Cancer Prevention, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Kiyono
authors.affiliation: Research Team for Geriatric Medicine (Vascular Medicine), Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
authors.forename: Masashi
authors.identifier: 0000-0002-9432-6401
authors.initials: M
authors.lastname: Toyoda
authors.affiliation: Laboratory for Medical Engineering, Division of Materials and Chemical Engineering, Graduate School of Engineering, Yokohama National University, Kanagawa 240-8501, Japan.
authors.forename: Masatoshi
authors.identifier: 0000-0002-9423-0901
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Department of Reproductive Biology, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan.
authors.forename: Akihiro
authors.identifier: 0000-0002-0485-6653
authors.initials: A
authors.lastname: Umezawa
mesh_terms: D001331:Automation; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D058732:Embryoid Bodies / Q000166:cytology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D015603:Keratinocytes / Q000166:cytology; D013724:Teratoma / Q000473:pathology; D013997:Time Factors