title: The SCN Clock Governs Circadian Transcription Rhythms in Murine Epididymal White Adipose Tissue.
abstract: The circadian master pacemaker in the suprachiasmatic nucleus (SCN) orchestrates peripheral clocks in various organs and synchronizes them with external time, including those in adipose tissue, which displays circadian oscillations in various metabolic and endocrine outputs. Because our knowledge about the instructive role of the SCN clock on peripheral tissue function is based mainly on SCN lesion studies, we here used an alternative strategy employing the Cre/ loxP system to functionally delete the SCN clock in mice. We performed whole-genome microarray hybridizations of murine epididymal white adipose tissue (eWAT) RNA preparations to characterize the role of the SCN clock in eWAT circadian transcriptome regulation. Most of the rhythmic transcripts in control animals were not rhythmic in SCN mutants, but a significant number of transcripts were rhythmic only in mutant eWAT. Core clock genes were rhythmic in both groups, but as was reported before for other tissues, rhythms were dampened and phase advanced in mutant animals. In SCN-mutant mice, eWAT lost the rhythm of metabolic pathway-related transcripts, while transcripts gaining rhythms in SCN-mutant mice were associated with various immune functions. These data reveal a complex interaction of SCN-derived and local circadian signals in the regulation of adipose transcriptome programs.
authors.affiliation: Chronophysiology Group, Medical Department 1, University of Lübeck, Lübeck, Germany.
authors.forename: Isa
authors.initials: I
authors.lastname: Kolbe
authors.affiliation: Program in Neurosciences and Mental Health, The Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: Jana
authors.initials: J
authors.lastname: Husse
authors.affiliation: Microarray and Deep-Sequencing Core Facility, Institute Developmental Biochemistry, University Medical Center, Göttingen, Germany.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Salinas
authors.affiliation: Microarray and Deep-Sequencing Core Facility, Institute Developmental Biochemistry, University Medical Center, Göttingen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lingner
authors.affiliation: Chronophysiology Group, Medical Department 1, University of Lübeck, Lübeck, Germany.
authors.forename: Mariana
authors.initials: M
authors.lastname: Astiz
authors.affiliation: Chronophysiology Group, Medical Department 1, University of Lübeck, Lübeck, Germany.
authors.forename: Henrik
authors.initials: H
authors.lastname: Oster
mesh_terms: D056930:ARNTL Transcription Factors / Q000235:genetics; D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics; D002940:Circadian Rhythm / Q000235:genetics*; D004822:Epididymis / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods; D063990:Gene Ontology; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*; D050857:Synaptotagmins / Q000235:genetics; D059467:Transcriptome / Q000235:genetics*
chemical_list: D056930:ARNTL Transcription Factors; C538961:Bmal1 protein, mouse; C558646:Syt10 protein, mouse; D050857:Synaptotagmins; D056926:CLOCK Proteins
keywords: circadian rhythm; epididymal white adipose tissue; microarray; suprachiasmatic nucleus; transcriptome