title: Improved specific loss power on cancer cells by hyperthermia and MRI contrast of hydrophilic FexCo1-xFe2O4nanoensembles.
abstract: Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity. Here we characterized nanoensembles of Fe-Co mixed spinel ferrites (i.e. FexCo1-xFe2O4, where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation. With nanoensembles of increasing Co content the saturation magnetization improved, while lattice parameter remained relatively constant. MRI water (transverse) relaxivity at 11.7 T was also boosted with increasing Co content. Efficiency of AMF-induced heating was quite comparable for the nanoensembles with either chitosan or polyethylene glycol (PEG) coating except for PEG-coated Fe0.2Co0.8Fe2O4, which was twice as less efficient as others. While toxicity of the nanoensembles with either coating examined on 9L tumor cell cultures showed no significant differences, upon AMF exposure (i.e. heat-induced necrosis) FexCo1-xFe2O4composition with different values of x showed quite dramatic effects on cell death of tumor cells with both coatings. This study lays the ground work for further characterization of other mixed spinel ferrites, and in addition we expect that chitosan and PEG coated FexCo1-xFe2O4of all the compositions will have good potential for preclinical applications in vivo. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Magnetic Resonance Research Center (MRRC) and Quantitative Neuroscience with Magnetic Resonance (QNMR) Core Center, Yale University, New Haven, CT, USA.|Department of Radiology & Biomedical Imaging, Yale University, New Haven, CT, USA.|Materials Science Division, Bangladesh Atomic Energy, Dhaka, Bangladesh.
authors.forename: S Manjura
authors.initials: SM
authors.lastname: Hoque
authors.affiliation: Magnetic Resonance Research Center (MRRC) and Quantitative Neuroscience with Magnetic Resonance (QNMR) Core Center, Yale University, New Haven, CT, USA.|Department of Radiology & Biomedical Imaging, Yale University, New Haven, CT, USA.
authors.forename: Yuegao
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University, New Haven, CT, USA.
authors.forename: Emiliano
authors.initials: E
authors.lastname: Cocco
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
authors.forename: Samuel
authors.initials: S
authors.lastname: Maritim
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University, New Haven, CT, USA.
authors.forename: Alessandro D
authors.initials: AD
authors.lastname: Santin
authors.affiliation: Department of Radiology, Michigan State University, East Lansing, MI, USA.
authors.forename: Erik M
authors.initials: EM
authors.lastname: Shapiro
authors.affiliation: Magnetic Resonance Research Center (MRRC) and Quantitative Neuroscience with Magnetic Resonance (QNMR) Core Center, Yale University, New Haven, CT, USA.|Department of Radiology & Biomedical Imaging, Yale University, New Haven, CT, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Coman
authors.affiliation: Magnetic Resonance Research Center (MRRC) and Quantitative Neuroscience with Magnetic Resonance (QNMR) Core Center, Yale University, New Haven, CT, USA.|Department of Radiology & Biomedical Imaging, Yale University, New Haven, CT, USA.|Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
authors.forename: Fahmeed
authors.initials: F
authors.lastname: Hyder
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D048271:Chitosan / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003035:Cobalt / Q000494:pharmacology; D003287:Contrast Media / Q000737:chemistry*; D005290:Ferric Compounds; D005334:Fever / Q000139:chemically induced* / Q000503:physiopathology; D006801:Humans; D007501:Iron; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry; D009336:Necrosis / Q000139:chemically induced* / Q000503:physiopathology; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000473:pathology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats
chemical_list: D020099:Coated Materials, Biocompatible; D003287:Contrast Media; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C001215:ferrite; D003035:Cobalt; D011092:Polyethylene Glycols; D048271:Chitosan; D007501:Iron
keywords: 9L gliosarcoma cells; MRI contrast agent; PEG; chitosan; hydrophilic; nanoensembles; nuclear magnetic resonance; specific loss power