title: Surface display on lactic acid bacteria without genetic modification: strategies and applications.
abstract: Microbial cell surface display has attracted greater attention than ever and has numerous potential applications in biotechnology. With the safety and probiotic properties, lactic acid bacteria (LAB) have been used widely in food and industrial applications. In order to circumvent using genetically modified microorganisms which face low public acceptance and severe regulatory scrutiny, surface-engineered LAB without genetical modification are more preferred. According to the way used to obtain the fusion protein containing the passenger molecule and anchoring domain, the genetic or chemical approaches can be used to construct these surface-engineered LAB. In addition to the viable wide-type LAB, non-living bacterial-like particles (BLP) can be attached by these fusion proteins added from outside. Compared to the living LAB, BLP have a higher binding capacity and less anticarrier response. Mucosal vaccines are the predominant application of these surface-engineered LAB with no genetical modification.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, People's Republic of China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, People's Republic of China.
authors.forename: Dongli
authors.initials: D
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, People's Republic of China. wangyefu@whu.edu.cn.
authors.forename: Yefu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D058956:Administration, Mucosal; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D061505:Cell Surface Display Techniques / Q000379:methods*; D056546:Lactobacillales / Q000737:chemistry*
chemical_list: D001428:Bacterial Vaccines
keywords: Bacteria-like particles; Lactic acid bacteria; Mucosal vaccine; Non-genetic modification; Surface display