title: Development and application of novelty pretreatment method for the concurrent quantitation of eleven water-soluble B vitamins in ultrafiltrates after renal replacement therapy.
abstract: Continous renal replacement therapy (CRRT) is particularly recommended for septic shock patients in intensive care units. The CRRT technique used most frequently is high volume continuous veno-venous haemofiltration. It provides a high rate of clearance of uremic toxins and inflammatory cytokines. However, it should also be taken into account that substances important for homeostasis may be concurrently unintentionally removed. Accordingly, water-soluble vitamins can be removed during continuous renal replacement therapy, and the estimate of the loss is critical to ensure appropriate supplementation. The aim of this work was to develop a simple methodology for a purification step prior to the LC-MS/MS determination of water-soluble vitamins in ultrafiltrate samples. For this purpose, two types of resin and a mix of resins were used as sorbents for the purification step. Moreover, parameters such as the amount of resin and the extraction time were optimized. The LC-MS/MS method was developed and validated for final determination of 11 vitamins. The results demonstrated the high purification capability of DEAE Sephadex resin with recoveries between 65 and 101% for water-soluble vitamins from ultrafiltrate samples. An optimized method was applied to assess the loss of B-group vitamins in patients after 24h of renal replacement therapy. The loss of vitamins B2, B6 pyridoxamine, B6 pyridoxal, B7, B1, and B5 in ultrafiltrates was similar in all patients. In the native ultrafiltrates, vitamins B6 pyridoxine, B9 and B12 were not detected.
authors.affiliation: University of Gdansk, Faculty of Chemistry, Department of Environmental Analysis, ul. Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Wirkus
authors.affiliation: University of Gdansk, Faculty of Chemistry, Department of Environmental Analysis, ul. Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Jakubus
authors.affiliation: Department of Anaesthesiology and Intensive Therapy, Medical University of Gdansk, ul. Mariana Smoluchowskiego 17, 80-214 Gdansk, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Owczuk
authors.affiliation: University of Gdansk, Faculty of Chemistry, Department of Environmental Analysis, ul. Wita Stwosza 63, 80-308 Gdansk, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Stepnowski
authors.affiliation: University of Gdansk, Faculty of Chemistry, Department of Environmental Analysis, ul. Wita Stwosza 63, 80-308 Gdansk, Poland. Electronic address: monika.paszkiewicz@ug.edu.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Paszkiewicz
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods*; D016014:Linear Models; D006435:Renal Dialysis; D017582:Renal Replacement Therapy; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014803:Vitamin B Complex / Q000032:analysis*
chemical_list: D014803:Vitamin B Complex
keywords: High volume continuous veno-venous haemofiltration; Ion exchange resins; LC–MS; Ultrafiltrates; Water-soluble vitamins