title: Real-Time Tracking of Parental Histones Reveals Their Contribution to Chromatin Integrity Following DNA Damage.
abstract: Chromatin integrity is critical for cell function and identity but is challenged by DNA damage. To understand how chromatin architecture and the information that it conveys are preserved or altered following genotoxic stress, we established a system for real-time tracking of parental histones, which characterize the pre-damage chromatin state. Focusing on histone H3 dynamics after local UVC irradiation in human cells, we demonstrate that parental histones rapidly redistribute around damaged regions by a dual mechanism combining chromatin opening and histone mobilization on chromatin. Importantly, parental histones almost entirely recover and mix with new histones in repairing chromatin. Our data further define a close coordination of parental histone dynamics with DNA repair progression through the damage sensor DDB2 (DNA damage-binding protein 2). We speculate that this mechanism may contribute to maintaining a memory of the original chromatin landscape and may help preserve epigenome stability in response to DNA damage.
authors.affiliation: Epigenetics & Cell Fate Centre, UMR7216 CNRS, Paris Diderot University, Sorbonne Paris Cité, F-75013 Paris, France; Laboratory of Nuclear Dynamics, UMR3664 CNRS, Institut Curie, PSL Research University, F-75005 Paris, France.
authors.forename: Salomé
authors.initials: S
authors.lastname: Adam
authors.affiliation: Epigenetics & Cell Fate Centre, UMR7216 CNRS, Paris Diderot University, Sorbonne Paris Cité, F-75013 Paris, France.
authors.forename: Juliette
authors.initials: J
authors.lastname: Dabin
authors.affiliation: Epigenetics & Cell Fate Centre, UMR7216 CNRS, Paris Diderot University, Sorbonne Paris Cité, F-75013 Paris, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Chevallier
authors.affiliation: Plateforme Imagerie Cellulaire et Tissulaire-Infrastructure en Biologie Santé et Agronomie, UMR3215 CNRS/U934 INSERM, Institut Curie, PSL Research University, F-75005 Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Leroy
authors.affiliation: Laboratory of Nuclear Dynamics, UMR3664 CNRS, Institut Curie, PSL Research University, F-75005 Paris, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Baldeyron
authors.affiliation: Team Chromatin Assembly, Nuclear Domains, Virus, Institut NeuroMyoGène, LabEx DEVweCAN, Université Claude Bernard Lyon 1, UMR5310 CNRS/U1217 INSERM, F-69100 Lyon, France.
authors.forename: Armelle
authors.initials: A
authors.lastname: Corpet
authors.affiliation: Team Chromatin Assembly, Nuclear Domains, Virus, Institut NeuroMyoGène, LabEx DEVweCAN, Université Claude Bernard Lyon 1, UMR5310 CNRS/U1217 INSERM, F-69100 Lyon, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Lomonte
authors.affiliation: Plateforme Imagerie Cellulaire et Tissulaire-Infrastructure en Biologie Santé et Agronomie, UMR3215 CNRS/U934 INSERM, Institut Curie, PSL Research University, F-75005 Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Renaud
authors.affiliation: Laboratory of Nuclear Dynamics, UMR3664 CNRS, Institut Curie, PSL Research University, F-75005 Paris, France.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Almouzni
authors.affiliation: Epigenetics & Cell Fate Centre, UMR7216 CNRS, Paris Diderot University, Sorbonne Paris Cité, F-75013 Paris, France. Electronic address: sophie.polo@univ-paris-diderot.fr.
authors.forename: Sophie E
authors.initials: SE
authors.lastname: Polo
mesh_terms: D045744:Cell Line, Tumor; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D042002:Chromatin Assembly and Disassembly; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods*; D042822:Genomic Instability; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D002843:Chromatin; C117669:DDB2 protein, human; D004268:DNA-Binding Proteins; D006657:Histones; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C086984:XPC protein, human