title: Culture of Tumorigenic Cells on Protein Fibers Reveals Metastatic Cell Behaviors.
abstract: Tumorigenic cell behaviors can be suppressed or enhanced by their physicochemical environment. As a first step toward developing materials that allow tumorigenic behaviors to be observed and manipulated, we cultured related MCF10 breast cell lines on fibers composed of the Drosophila protein Ultrabithorax (Ubx). These cell lines, originally derived from fibrocystic breast tissue, represent a continuum of tumorigenic behavior. Immortal but nontumorigenic MCF10A cells, as well as semitumorigenic MCF10AT cells, attached and spread on Ubx fibers. MCF10CA-1a cells, the most highly transformed line, secreted high concentrations of matrix metalloproteinases when cultured on Ubx materials, resulting in differences in cell attachment and cytoskeletal structure, and enabling invasive behavior. Because the mechanical and functional properties of Ubx fibers can be genetically manipulated, these materials provide a valuable tool for cancer research, allowing creation of diverse microenvironments that allow assessment of invasive, metastatic behavior.
authors.affiliation: Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center , College Station, Texas 77843, United States.|Department of Biosciences, Rice University , Houston Texas 77251, United States.
authors.forename: Hao-Ching
authors.initials: HC
authors.lastname: Hsiao
authors.affiliation: Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center , College Station, Texas 77843, United States.|Department of Biosciences, Rice University , Houston Texas 77251, United States.
authors.forename: Andres
authors.initials: A
authors.lastname: Santos
authors.affiliation: Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center , College Station, Texas 77843, United States.|Department of Biosciences, Rice University , Houston Texas 77251, United States.
authors.forename: David W
authors.initials: DW
authors.lastname: Howell
authors.affiliation: Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center , College Station, Texas 77843, United States.|Department of Biosciences, Rice University , Houston Texas 77251, United States.
authors.forename: Jan L
authors.initials: JL
authors.lastname: Patterson
authors.affiliation: Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center , College Station, Texas 77843, United States.|Department of Biosciences, Rice University , Houston Texas 77251, United States.
authors.forename: Robin S L
authors.initials: RS
authors.lastname: Fuchs-Young
authors.affiliation: Department of Molecular and Cellular Medicine, Texas A&M University Health Science Center , College Station, Texas 77843, United States.|Department of Biosciences, Rice University , Houston Texas 77251, United States.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Bondos
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor / Q000187:drug effects*; D029721:Drosophila Proteins / Q000737:chemistry*; D004331:Drosophila melanogaster / Q000737:chemistry; D005260:Female; D018398:Homeodomain Proteins / Q000737:chemistry*; D006801:Humans; D009362:Neoplasm Metastasis / Q000473:pathology; D014157:Transcription Factors / Q000737:chemistry*
chemical_list: D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D014157:Transcription Factors; C066177:Ubx protein, Drosophila