title: The Effect of Silver Carp Skin-Derived Peptides on the Activities of VEGF and Hsp70.1 Gene Transcription Promoters.
abstract: BACKGROUND
  Studies conducted on human cell culture models have demonstrated that collagen-derived peptides can exert a beneficial effect in medicine. However, all these studies were conducted using animal collagen samples, most often originating from bovine or porcine skin. Currently attempts are being made to replace animal collagen with fish collagen.
  
  
  OBJECTIVE
  The aim of the study was to compare the effect of silver carp skin-derived peptide extract on the transcriptional activities of human VEGF and hsp70.1 gene promoters inserted into the plasmids with secreted alkaline phosphatase as a reporter gene.
  
  
  METHODS
  Changes in the activity of the promoters were investigated using a HEK293FT cell line transfected with pVEGF-SEAP or pHsp70-SEAP. The cells were cultured in dishes containing peptides separated using reverse-phase high performance liquid chromatography.
  
  
  RESULTS
  The study demonstrated that the silver carp skin-derived peptide extract exerts both an inhibitory effect on the VEGF gene promoter and activating effect on the hsp70.1 gene promoter. Higher biological activity was recorded in the case of a freshly prepared peptide extract compared to one stored at 4°C for three months.
  
  
  CONCLUSIONS
  The silver carp skin-derived collagen peptides influence VEGF and hsp70.1 gene promoters' transcriptional activity.
authors.affiliation: Institute of Human Physiology, Faculty of Health Sciences, Medical University of Gdańsk, Poland.
authors.forename: Diana
authors.initials: D
authors.lastname: Wojtkowiak
authors.affiliation: Institute of Human Physiology, Faculty of Health Sciences, Medical University of Gdańsk, Poland.
authors.forename: Janina
authors.initials: J
authors.lastname: Piechowicz
authors.affiliation: Biotechnological Laboratory, Pomeranian Science and Technology Park, Gdynia Innovation Center, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Grzenkowicz-Wydra
authors.affiliation: Laboratory of EPR Tomography, Institute of Materials Technology, Poznan University of Technology, Poland.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Wosiński
authors.affiliation: Department of Dental Surgery, Wroclaw Medical University, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Dominiak
authors.affiliation: Department of Dental Surgery, Wroclaw Medical University, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Hadzik
authors.affiliation: Institute of Human Physiology, Faculty of Health Sciences, Medical University of Gdańsk, Poland.
authors.forename: Andrzej F
authors.initials: AF
authors.lastname: Frydrychowski
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002347:Carps; D003094:Collagen / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D010455:Peptides / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012867:Skin / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D029941:Fish Proteins; D018840:HSP70 Heat-Shock Proteins; D010455:Peptides; D042461:Vascular Endothelial Growth Factor A; C081884:heat-shock protein 70.1; D003094:Collagen; D000469:Alkaline Phosphatase
keywords: collagen; fish collagen; gene promoters; skin-derived peptide