title: Effects of Koumine on Adjuvant- and Collagen-Induced Arthritis in Rats.
abstract: To examine the effect of koumine, a Gelsemium alkaloid, on two experimental models of rheumatoid arthritis (RA), rats with adjuvant-induced arthritis (AIA) and collagen-induced arthritis (CIA) were administered koumine (0.6, 3, or 15 mg/kg/day) or vehicle through gastric gavage (i.g.). Clinical evaluation was performed via measurements of hind paw volume, arthritis index (AI) score, mechanical withdrawal threshold, organ weight, and by radiographic and histological examinations. Levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and antitype II collagen (CII) antibody were also examined. In rats with AIA, koumine reduced the AI score and mechanical allodynia of the injected hind paw in a dose-dependent manner and significantly inhibited increase in thymus and liver weights. In rats with CIA, koumine inhibited increase in hind paw volume, AI score, and mechanical allodynia in a dose-dependent manner and reduced joint space narrowing. Furthermore, koumine also attenuated the increase in the expression of IL-1β and TNF-α, as well as the robust increase of serum anti-CII antibodies in response to immunization. These results suggested that koumine effectively attenuated arthritis progression in two rat models of RA and that this therapeutic effect may be associated with its immunoregulatory action.
authors.affiliation: Department of Pharmacology and ‡Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University , Fuzhou, Fujian 350108, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Pharmacology and ‡Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University , Fuzhou, Fujian 350108, China.
authors.forename: Hong-Da
authors.initials: HD
authors.lastname: Cai
authors.affiliation: Department of Pharmacology and ‡Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University , Fuzhou, Fujian 350108, China.
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Zeng
authors.affiliation: Department of Pharmacology and ‡Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University , Fuzhou, Fujian 350108, China.
authors.forename: Ze-Hong
authors.initials: ZH
authors.lastname: Chen
authors.affiliation: Department of Pharmacology and ‡Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University , Fuzhou, Fujian 350108, China.
authors.forename: Meng-Han
authors.initials: MH
authors.lastname: Fang
authors.affiliation: Department of Pharmacology and ‡Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University , Fuzhou, Fujian 350108, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Su
authors.affiliation: Department of Pharmacology and ‡Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University , Fuzhou, Fujian 350108, China.
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Huang
authors.affiliation: Department of Pharmacology and ‡Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University , Fuzhou, Fujian 350108, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Pharmacology and ‡Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University , Fuzhou, Fujian 350108, China.
authors.forename: Chang-Xi
authors.initials: CX
authors.lastname: Yu
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000276:immunology*; D003094:Collagen / Q000494:pharmacology*; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000188:drug therapy; D005260:Female; D031450:Gelsemium / Q000737:chemistry*; D026121:Indole Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000032:analysis; D008297:Male; D008727:Methotrexate / Q000494:pharmacology; D015394:Molecular Structure; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D016207:Cytokines; D026121:Indole Alkaloids; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; C515085:koumine; D003094:Collagen; D008727:Methotrexate