title: Study on quantitative expression of PPARγ and ADRP in muscle and its association with intramuscular fat deposition of pig.
abstract: BACKGROUND
  Intramuscular fat (intramuscular fat, IMF) is one of the important traits of pork quality. How to reasonably improve the intramuscular fat content is the most focus researchers. Some possible regulation of intramuscular fat deposition of candidate genes to cause the attention of people. The objective of this study was to elucidate the relationship between peroxisome proliferator-activated receptor γ (PPARγ) and adipose differentiation-related protein (ADRP) mRNA expression and intramuscular fat (IMF) deposition in the muscle tissue of three breeds of pig: Laiwu (LW), Lulai Black (LL), and Large White (LY).
  
  
  RESULTS
  qPCR analysis of the PPARγ and ADRP genes in the three breeds of pig revealed PPARγ and ADRP mRNA expression profiles of LW > LL > LY and LL > LW > LY, respectively. PPARγ mRNA expression was significantly and positively correlated with IMF deposition (p < 0.05). There were significant correlations between PPARγ and ADRP mRNA expression levels (p < 0.01).
  
  
  CONCLUSIONS
  These results suggest correlations between PPARγ and ADRP in fat deposition and regulation in pigs, PPARγ gene may be a main effector of IMF content and play an important role during adipocyte differentiation in pigs, thereby providing new information to further elucidate molecular mechanisms associated with intramuscular fat deposition in Laiwu pigs and provides new data for further molecular studies of mechanisms underlying intramuscular fat deposition in human obesity. The continued elucidation of specific genetic mechanisms between PPARγ and ADRP warrants further studies.
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018 China.|Weifang University of Science and Technology, Shouguang, 262700 Shandong China.
authors.forename: Jingxiang
authors.identifier: 0000-0002-1808-8501
authors.initials: J
authors.lastname: Cui
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018 China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Weifang University of Science and Technology, Shouguang, 262700 Shandong China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093 China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018 China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Zeng
keywords: ADRP; Fat deposition; PPARγ; Pig; QRT-PCR