title: High-efficient and high-content cytotoxic recording via dynamic and continuous cell-based impedance biosensor technology.
abstract: Cell-based bioassays were effective method to assess the compound toxicity by cell viability, and the traditional label-based methods missed much information of cell growth due to endpoint detection, while the higher throughputs were demanded to obtain dynamic information. Cell-based biosensor methods can dynamically and continuously monitor with cell viability, however, the dynamic information was often ignored or seldom utilized in the toxin and drug assessment. Here, we reported a high-efficient and high-content cytotoxic recording method via dynamic and continuous cell-based impedance biosensor technology. The dynamic cell viability, inhibition ratio and growth rate were derived from the dynamic response curves from the cell-based impedance biosensor. The results showed that the biosensors has the dose-dependent manners to diarrhetic shellfish toxin, okadiac acid based on the analysis of the dynamic cell viability and cell growth status. Moreover, the throughputs of dynamic cytotoxicity were compared between cell-based biosensor methods and label-based endpoint methods. This cell-based impedance biosensor can provide a flexible, cost and label-efficient platform of cell viability assessment in the shellfish toxin screening fields.
authors.affiliation: Key Laboratory of Biomedical Engineering of Ministry of Education, Biosensor National Special Laboratory, Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China. huning@zju.edu.cn.|State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050, China. huning@zju.edu.cn.|Division of Biomedical Engineering, Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA. huning@zju.edu.cn.|Harvard-MIT Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. huning@zju.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Hu
authors.affiliation: Key Laboratory of Biomedical Engineering of Ministry of Education, Biosensor National Special Laboratory, Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China.
authors.forename: Jiaru
authors.initials: J
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Diagnostic and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, 48824, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Wan
authors.affiliation: Key Laboratory of Biomedical Engineering of Ministry of Education, Biosensor National Special Laboratory, Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Key Laboratory of Biomedical Engineering of Ministry of Education, Biosensor National Special Laboratory, Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China.
authors.forename: Kaiqi
authors.initials: K
authors.lastname: Su
authors.affiliation: Key Laboratory of Biomedical Engineering of Ministry of Education, Biosensor National Special Laboratory, Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biomedical Engineering of Ministry of Education, Biosensor National Special Laboratory, Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China.|State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D049872:Bivalvia; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000633:toxicity*; D017097:Electric Impedance; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D018675:Toxicity Tests / Q000379:methods*; D014118:Toxins, Biological / Q000633:toxicity
chemical_list: D003603:Cytotoxins; D014118:Toxins, Biological
keywords: Cell growth rate; Cell viability; Cell-based impedance biosensor; Dynamic and continuous recording; Okadiac acid