title: A computational lens for sexual-stage transmission, reproduction, fitness and kinetics in Plasmodium falciparum.
abstract: BACKGROUND
  The burden of falciparum malaria remains unacceptably high in much of sub-Saharan Africa and massive efforts are underway to eliminate the parasite. While symptoms of malaria are caused by asexual reproduction of the parasite, transmission to new human hosts relies entirely on male and female sexual-stage parasites, known as gametocytes. Successful transmission can be observed at very low gametocyte densities, which raises the question of whether transmission-enhancing mechanisms exist in the human host, the mosquito, or both.
  
  
  METHODS
  A new computational model was developed to investigate the probability of fertilization over a range of overdispersion parameters and male gamete exploration rates. Simulations were used to fit a likelihood surface for data on rates of mosquito infection across a wide range of host gametocyte densities.
  
  
  RESULTS
  The best fit simultaneously requires very strong overdispersion and faster gamete exploration than is possible with random swimming in order to explain typical prevalence levels in mosquitoes. Gametocyte overdispersion or clustering in the human host and faster gamete exploration of the mosquito blood meal are highly probably given these results.
  
  
  CONCLUSIONS
  Density-dependent gametocyte clustering in the human host, and non-random searching (e.g., chemotaxis) in the mosquito are probable. Future work should aim to discover these mechanisms, as disrupting parasite development in the mosquito will play a critical role in eliminating malaria.
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK.
authors.forename: Mara K N
authors.initials: MK
authors.lastname: Lawniczak
authors.affiliation: Institute for Disease Modeling, 3150 139th Avenue SE, Bellevue, WA, 98005, USA. peckhoff@intven.com.
authors.forename: Philip A
authors.initials: PA
authors.lastname: Eckhoff
keywords: Gametocytes; Mathematical model; Plasmodium falciparum