title: Ethanol Extract of Fructus ligustri lucidi Increased Circulating 1,25(OH)2D3Levels, but Did Not Improve Calcium Balance in Mature Ovariectomized Rats.
abstract: Our previous studies found that different extracts or fractions of Fructus ligustri lucidi (FLL) played different roles in altering the regulation of bone and mineral metabolism in different animal models. The present study was designed to compare the actions of FLL ethanol (EE) and water extracts (WE) on bone and mineral metabolism in a 6-month-old mature ovariectomized (OVX) rat model. Our results showed that FLL extracts did not significantly improve systematic Ca balance in mature OVX rats. However, EE, but not WE treatment, significantly increased serum 1,25(OH)2D3levels in mature OVX rats. An in vitro study using human proximal tubule (HKC-8) cells showed that EE, but not WE, significantly enhanced renal 25-dihydroxyvitamin D3-1[Formula: see text]-hydroxylase (1-OHase) mRNA expressions and simultaneously repressed renal 25-dihydroxyvitamin D3-24-hydroxylase (24-OHase) mRNA expressions. Further investigation indicated that EE could significantly induce the protein expression of 1-OHase, but did not alter 24-OHase expression in HKC-8 cells. Our results demonstrated that EE increased circulating 1,25(OH)2D3levels in OVX rats, possibly via upregulation of renal 1-OHase expressions in renal proximal tubule cells. Our study indicates that FLL is a natural oral agent that could directly regulate renal vitamin D metabolism in vivo and in vitro.
authors.affiliation: * Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P.R. China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Dong
authors.affiliation: * Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P.R. China.
authors.forename: Si-Si
authors.initials: SS
authors.lastname: Cao
authors.affiliation: * Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P.R. China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zhou
authors.affiliation: § Nestlé Research Centre, P. O. Box 44, 1000 Lausanne 26, Switzerland.
authors.forename: Liya
authors.initials: L
authors.lastname: Denney
authors.affiliation: * Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P.R. China.|‡ Shenzhen Research Institute of The Hong Kong Polytechnic University, State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen 518057, P.R. China.
authors.forename: Man-Sau
authors.initials: MS
authors.lastname: Wong
authors.affiliation: † Nestlé Research Centre Beijing, No. 5 Dijin Road, Haidian District, Beijing, P.R. China.
authors.forename: Hao-Tian
authors.initials: HT
authors.lastname: Feng
mesh_terms: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002117:Calcitriol / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D000431:Ethanol; D005260:Female; D005638:Fruit / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism; D031659:Ligustrum / Q000737:chemistry*; D023421:Models, Animal; D010052:Ovariectomy; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000187:drug effects; D065668:Vitamin D3 24-Hydroxylase / Q000235:genetics / Q000378:metabolism; D014867:Water
chemical_list: D010936:Plant Extracts; D014867:Water; D000431:Ethanol; C585551:Cyp24a1 protein, rat; D065668:Vitamin D3 24-Hydroxylase; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; D002117:Calcitriol; D002118:Calcium
keywords: 1,25(OH)2D3; 25-dihydroxyvitamin D3-1-hydroxylase; 25-dihydroxyvitamin D3-24-hydroxylase; Fructus ligustri lucidi; Intestinal Ca Absorption