title: Lipid profiles of canine spermatozoa as revealed via matrix-assisted laser desorption/ionization mass spectrometry.
abstract: In this study, we investigated the ability of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to characterize the lipid contents of canine spermatozoa. For that, samples of pure semen were analysed. Indeed, quite comprehensive lipid coverage was observed, and the most abundant phospholipid ions detected were from four phosphatidylcholines, that is those of m/z 760.6; 782.6; 808.6; and 830.6 and one of m/z 725.6 from a sphingomyelin. In conclusion, MALDI-MS was found to offer an easy, fast, accurate, and sensitive analytical method for lipid profiling in canine spermatozoa and could be used as a tool to select sires by assessing the relationship between sperm lipid profiles and variables such as age and breeding history as well as to study the effects of cryopreservation on lipid contents.
authors.affiliation: Animal Reproduction Laboratory, Animal Science Graduate Program, University of Franca (UNIFRAN), Franca, São Paulo, Brazil.
authors.forename: L T
authors.initials: LT
authors.lastname: Braga
authors.affiliation: Animal Reproduction Laboratory, Animal Science Graduate Program, University of Franca (UNIFRAN), Franca, São Paulo, Brazil.
authors.forename: Nrs
authors.initials: N
authors.lastname: Bravo
authors.affiliation: ThoMSon Mass Spectrometry Laboratory, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Kra
authors.initials: K
authors.lastname: Belaz
authors.affiliation: ThoMSon Mass Spectrometry Laboratory, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Zampieri
authors.affiliation: ThoMSon Mass Spectrometry Laboratory, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: M N
authors.initials: MN
authors.lastname: Eberlin
authors.affiliation: Animal Reproduction Laboratory, Animal Science Graduate Program, University of Franca (UNIFRAN), Franca, São Paulo, Brazil.
authors.forename: V A
authors.identifier: http://orcid.org/0000-0002-1991-6786
authors.initials: VA
authors.lastname: Conforti
mesh_terms: D000818:Animals; D004285:Dogs / Q000502:physiology*; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008297:Male; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary*; D013094:Spermatozoa / Q000737:chemistry* / Q000502:physiology
chemical_list: D008055:Lipids