title: Low temperature and Daphnia-associated infochemicals promote colony formation of Scenedesmus obliquus and its harvesting.
abstract: OBJECTIVE
  To explore the combined effects of temperature and Daphnia-associated infochemicals on colony formation of Scenedesmus obliquus to faciliate harvesting the algal biomass.
  
  
  RESULTS
  A three-parameter modified Gaussian model fitted the changes of the number of cells per particle in S. obliquus induced by Daphnia culture filtrate well under any temperature. Decreases in temperature enhanced the induced-colony formation of Scenedesmus. The maximum colony size at 15-25 °C was significantly larger than those at 30-35 °C. An additional 1 or 2 days at low temperature was needed to reach the maximum colony size, which indicates the best harvest time for algal biomass.
  
  
  CONCLUSIONS
  Induced-colony formation of Scenedesmus by Daphnia culture filtrate at 15-25 °C is recommended to settle algal cells. This condition facilitates harvesting the biomass.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological SciencesNanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China.
authors.forename: Xuexia
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological SciencesNanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Yang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological SciencesNanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological SciencesNanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological SciencesNanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological SciencesNanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China. huangyuan@njnu.edu.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological SciencesNanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D018533:Biomass; D003080:Cold Temperature; D003621:Daphnia / Q000378:metabolism*; D044444:Scenedesmus / Q000378:metabolism; D013696:Temperature
keywords: Colony formation; Infochemicals; Microalgal harvest; Scenedesmus obliquus; Temperature