title: Expression and localization of forkhead box protein FOXJ1 in S100β-positive multiciliated cells of the rat pituitary.
abstract: S100β-positive cells exist in the marginal cell layer (MCL) of the adenohypophysis and follicle structure in the parenchyma of anterior lobe (ALFS) in pituitary. They have multiple functions as phagocytes or cells that regulate hormone secretion. Majority of S100β-positive cells in the adenohypophysis express sex determining region Y-box 2 protein (SOX2), a stem cell marker; therefore, S100β/SOX2 double positive cells are also considered as one type of stem/progenitor cells. MCL and ALFS are consisting of morphologically two types of cells, i.e., multiciliated cells and non-ciliated cells. However, the relationship between the S100β-positive cells and multiciliated cells in the pituitary is largely unknown. In the present study, we first immunohistochemically verified the feature of multiciliated cells in MCL and ALFS. We then examined the expression patterns of FOXJ1, an essential expression factor for multiciliated cell-differentiation, and SOX2 in the S100β-positive multiciliated cells by in situ hybridization and immunohistochemistry. We identified anew the S100β/SOX2/FOXJ1 triple positive multiciliated cells, and revealed that they were dispersed throughout the MCL and ALFS. These results indicate that the MCL and ALFS are consisting of morphologically and functionally distinct two types of cells, i.e., S100β/SOX2 double positive non-ciliated cells and S100β/SOX2/FOXJ1 triple positive multiciliated cells.
authors.affiliation: Department of Anatomy and Cell Biology, Teikyo University School of Medicine, 2-11-1 Kaga Itabashi-Ku, Tokyo, 173-8605, Japan. nakakura@med.teikyo-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakakura
authors.affiliation: Department of Biology, Sapporo Medical University, Sapporo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Laboratory of Anatomy and Cell Biology, Department of Health Sciences, Kyorin University, Tokyo, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Horiguchi
authors.affiliation: Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine, Tochigi, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Fujiwara
authors.affiliation: Department of Biomolecular Science, Faculty of Science, Toho University, Chiba, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Tsukada
authors.affiliation: Department of Anatomy and Cell Biology, Teikyo University School of Medicine, 2-11-1 Kaga Itabashi-Ku, Tokyo, 173-8605, Japan.
authors.forename: Anshin
authors.initials: A
authors.lastname: Asano-Hoshino
authors.affiliation: Department of Anatomy and Cell Biology, Teikyo University School of Medicine, 2-11-1 Kaga Itabashi-Ku, Tokyo, 173-8605, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Department of Anatomy and Cell Biology, Teikyo University School of Medicine, 2-11-1 Kaga Itabashi-Ku, Tokyo, 173-8605, Japan.
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Arisawa
authors.affiliation: Department of Anatomy and Cell Biology, Teikyo University School of Medicine, 2-11-1 Kaga Itabashi-Ku, Tokyo, 173-8605, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Nishijima
authors.affiliation: Department of Anatomy and Cell Biology, Teikyo University School of Medicine, 2-11-1 Kaga Itabashi-Ku, Tokyo, 173-8605, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nekooki-Machida
authors.affiliation: Department of Anatomy and Cell Biology, Teikyo University School of Medicine, 2-11-1 Kaga Itabashi-Ku, Tokyo, 173-8605, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Kiuchi
authors.affiliation: Department of Anatomy and Cell Biology, Teikyo University School of Medicine, 2-11-1 Kaga Itabashi-Ku, Tokyo, 173-8605, Japan.
authors.forename: Haruo
authors.initials: H
authors.lastname: Hagiwara
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002923:Cilia / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D010903:Pituitary Gland, Anterior / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D064568:S100 Calcium Binding Protein beta Subunit / Q000235:genetics* / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics* / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: C500666:FOXJ1 protein, rat; D051858:Forkhead Transcription Factors; D064568:S100 Calcium Binding Protein beta Subunit; D055748:SOXB1 Transcription Factors; C527784:Sox2 protein, rat
keywords: FOXJ1; Multiciliated cells; Pituitary; S100β; SOX2