title: Purification and characterization of a novel alginate lyase from the marine bacterium Cobetia sp. NAP1 isolated from brown algae.
abstract: The application of marine resources, instead of fossil fuels, for biomass production is important for building a sustainable society. Seaweed is valuable as a source of marine biomass for producing biofuels such as ethanol, and can be used in various fields. Alginate is an anionic polysaccharide that forms the main component of brown algae. Various alginate lyases (e.g. exo- and endo-types and oligoalginate lyase) are generally used to degrade alginate. We herein describe a novel alginate lyase, AlgC-PL7, which belongs to the polysaccharide lyase 7 family. AlgC-PL7 was isolated from the halophilic Gram-negative bacterium Cobetia sp. NAP1 collected from the brown algae Padina arborescens Holmes. The optimal temperature and pH for AlgC-PL7 activity were 45 °C and 8, respectively. Additionally, AlgC-PL7 was thermostable and salt-tolerant, exhibited broad substrate specificity, and degraded alginate into monosaccharides. Therefore, AlgC-PL7 is a promising enzyme for the production of biofuels.
authors.affiliation: a Center for Research on Green Sustainable Chemistry , Tottori University , Tottori , Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Yagi
authors.affiliation: b Department of Chemistry and Biotechnology , Graduate School of Engineering, Tottori University , Tottori , Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Fujise
authors.affiliation: c Faculty of Engineering, Department of Biotechnology , Tottori University , Tottori , Japan.
authors.forename: Narumi
authors.initials: N
authors.lastname: Itabashi
authors.affiliation: b Department of Chemistry and Biotechnology , Graduate School of Engineering, Tottori University , Tottori , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohshiro
mesh_terms: D000464:Alginates / Q000378:metabolism; D000595:Amino Acid Sequence; D004795:Enzyme Stability; D020723:Glucuronic Acid / Q000378:metabolism; D044129:Halomonadaceae / Q000201:enzymology*; D006603:Hexuronic Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009005:Monosaccharides / Q000378:metabolism; D000459:Phaeophyceae / Q000382:microbiology*; D011133:Polysaccharide-Lyases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D009005:Monosaccharides; D012965:Sodium Chloride; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase
keywords: Cobetia sp; PL 7 family; alginate lyase; characterization; purification