title: Surveillance of Avian Influenza Virus in Aquatic Birds on the Brazilian Amazon Coast.
abstract: The occurrence of avian influenza viruses (AIV) has been extensively studied in aquatic birds in the Northern hemisphere; however, much less information is available for the South American region. In 2009-2010, we sampled 1006 wild aquatic birds (90% Charadriiformes, 9% Anseriformes, and 1% other groups) at three locations on the Brazilian Amazon coast, a region that serves as a major stop-over and wintering site along the Atlantic Americas flyway. Real-time RT-PCR identified five samples as positive; however, no AIV isolates could be obtained and Illumina sequencing did not produce gene sequences that would allow further characterization of the virus.
authors.affiliation: Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil. renata_fh@yahoo.com.br.|Laboratory Biosafety Level 3+, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. renata_fh@yahoo.com.br.|, Av. Silvestre 103, Condomínio Arujazinho IV, Arujá, SP, CEP: 07434-530, Brazil. renata_fh@yahoo.com.br.
authors.forename: Renata
authors.initials: R
authors.lastname: Hurtado
authors.affiliation: Department of Zoology, Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Severino Mendes
authors.initials: SM
authors.lastname: de Azevedo-Júnior
authors.affiliation: Laboratory of Wildlife Comparative Pathology, Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Ralph Eric Thijl
authors.initials: RE
authors.lastname: Vanstreels
authors.affiliation: Division of Virology, Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Fabrizio
authors.affiliation: Division of Virology, Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA.
authors.forename: David
authors.initials: D
authors.lastname: Walker
authors.affiliation: Department of Zoology, Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Roberta C
authors.initials: RC
authors.lastname: Rodrigues
authors.affiliation: Laboratory Biosafety Level 3+, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Marina M M
authors.initials: MM
authors.lastname: Seixas
authors.affiliation: Laboratory Biosafety Level 3+, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Jansen
authors.initials: J
authors.lastname: de Araújo
authors.affiliation: Laboratory Biosafety Level 3+, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Luciano M
authors.initials: LM
authors.lastname: Thomazelli
authors.affiliation: Laboratory Biosafety Level 3+, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Tatiana Lopes
authors.initials: TL
authors.lastname: Ometto
authors.affiliation: Laboratory of Wildlife Comparative Pathology, Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Webby
authors.affiliation: Laboratory of Wildlife Comparative Pathology, Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Robert G
authors.initials: RG
authors.lastname: Webster
authors.affiliation: Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Jerez
authors.affiliation: Laboratory Biosafety Level 3+, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Edison Luiz
authors.initials: EL
authors.lastname: Durigon
mesh_terms: D000818:Animals; D000835:Animals, Wild; D046090:Anseriformes / Q000821:virology*; D001717:Birds; D001938:Brazil / Q000453:epidemiology; D009980:Influenza A virus / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000453:epidemiology*
keywords: Anseriformes; Brazil; Charadriiformes; influenzavirus; migratory birds