title: Clinical Effects of Gamma-Radiation-Resistant Aspergillus sydowii on Germ-Free Mice Immunologically Prone to Inflammatory Bowel Disease.
abstract: We report and investigated a case of inadvertent contamination of 125 mice (housed in two germ-free positive-pressurized isolators) with emerging human and coral pathogen Aspergillus sydowii. The infected mice correspond to genetic line SAMP1/YitFc, which have 100% immune predisposition to develop Crohn's disease-like spontaneous pathologies, namely, inflammatory bowel disease (IBD). Pathogen update based on a scoping review of the literature and our clinical observations and experimentation are discussed. The unwanted infection of germ-free mice (immunologically prone to suffer chronic inflammation) with human pathogen A. sydowii resulted in no overt signs of clinical disease over 3-week exposure period, or during DSS-induced colitis experiments. Results and observations suggest that A. sydowii alone has limited clinical effect in immunocompromised germ-free mice or that other commensal microbial flora is required for Aspergillus-associated disease to occur.
authors.affiliation: Division of Gastroenterology and Liver Diseases, Department of Medicine, Digestive Health Research Institute, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA; Cleveland Digestive Diseases Research Core Center, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
authors.forename: Alexander
authors.identifier: 0000-0003-0713-5605
authors.initials: A
authors.lastname: Rodriguez-Palacios
authors.affiliation: Division of Gastroenterology and Liver Diseases, Department of Medicine, Digestive Health Research Institute, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA; Cleveland Digestive Diseases Research Core Center, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Aladyshkina
authors.affiliation: Center for Medical Mycology, Department of Dermatology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Retuerto
authors.affiliation: Center for Medical Mycology, Department of Dermatology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Hager
authors.affiliation: Department of Human Sciences, Human Nutrition, College of Education and Human Ecology, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Sanja
authors.identifier: 0000-0003-3450-2693
authors.initials: S
authors.lastname: Ilic
authors.affiliation: Center for Medical Mycology, Department of Dermatology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA; Digestive Health Institute, University Hospitals Case Medical Center, Cleveland, OH 44106, USA.
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Ghannoum
authors.affiliation: Division of Gastroenterology and Liver Diseases, Department of Medicine, Digestive Health Research Institute, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA; Cleveland Digestive Diseases Research Core Center, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA; Digestive Health Institute, University Hospitals Case Medical Center, Cleveland, OH 44106, USA.
authors.forename: Fabio
authors.initials: F
authors.lastname: Cominelli