title: Gut microbiota Modulated by Probiotics and Garcinia cambogia Extract Correlate with Weight Gain and Adipocyte Sizes in High Fat-Fed Mice.
abstract: Results of recent studies on gut microbiota have suggested that obesogenic bacteria exacerbate obesity and metabolic dysfunction in the host when fed a high fat diet (HFD). In order to explore obesity-associated bacterial candidates and their response to diet, the composition of faecal bacterial communities was investigated by analyzing 16S rRNA gene sequences in mice. Dietary intervention with probiotics and Garcinia cambogia extract attenuated weight gain and adipocyte size in HFD-fed mice. To identify obesity-causative microbiota, two statistical analyses were performed. Forty-eight bacterial species were found to overlap between the two analyses, indicating the commonly identified species as diet-driven and obesity-associated, which would make them strong candidates for host-microbiome interaction on obesity. Finally, correlation based network analysis between diet, microbe, and host revealed that Clostridium aminophilum, a hyper-ammonia-producing bacterium, was highly correlated with obesity phenotypes and other associated bacteria, and shown to be suppressed by the combination of probiotics and Garcinia cambogia extract. Results of the present study suggest that probiotics and Garcinia cambogia extract alleviate weight gain and adiposity, in part via differentially modulating the composition of gut microbiota in HFD fed mice.
authors.affiliation: C&K genomics, Seoul National University Research Park, Seoul 151-919, Republic of Korea.
authors.forename: Jaeyoung
authors.initials: J
authors.lastname: Heo
authors.affiliation: C&K genomics, Seoul National University Research Park, Seoul 151-919, Republic of Korea.|Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Minseok
authors.initials: M
authors.lastname: Seo
authors.affiliation: Laboratory of Developmental Genetics, Department of Biomedical Sciences, Inha University School of Medicine, Incheon 22212, Republic of Korea.
authors.forename: Hwanhee
authors.initials: H
authors.lastname: Park
authors.affiliation: Laboratory of Developmental Genetics, Department of Biomedical Sciences, Inha University School of Medicine, Incheon 22212, Republic of Korea.
authors.forename: Woon Kyu
authors.initials: WK
authors.lastname: Lee
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: Le Luo
authors.initials: LL
authors.lastname: Guan
authors.affiliation: Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Joon
authors.initials: J
authors.lastname: Yoon
authors.affiliation: Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Kelsey
authors.initials: K
authors.lastname: Caetano-Anolles
authors.affiliation: Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Hyeonju
authors.initials: H
authors.lastname: Ahn
authors.affiliation: R&D center, CTCBIO, Inc. 38, Hyundaikia-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18576, Republic of Korea.
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: R&D center, CTCBIO, Inc. 38, Hyundaikia-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18576, Republic of Korea.
authors.forename: Yoon-Mo
authors.initials: YM
authors.lastname: Kang
authors.affiliation: C&K genomics, Seoul National University Research Park, Seoul 151-919, Republic of Korea.
authors.forename: Seoae
authors.initials: S
authors.lastname: Cho
authors.affiliation: C&K genomics, Seoul National University Research Park, Seoul 151-919, Republic of Korea.|Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul 151-742, Republic of Korea.|Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Heebal
authors.initials: H
authors.lastname: Kim