title: Quick cytogenetic screening of breeding bulls using flow cytometric sperm DNA histogram analysis.
abstract: The aim of the present study was to test the FXCycle PI/RNase kit for routine DNA analyses in order to detect breeding bulls and/or insemination doses carrying cytogenetic aberrations. In a series of experiments we first established basic DNA histogram parameters of cytogenetically healthy breeding bulls by measuring the intraspecific genome size variation of three animals, then we compared the histogram profiles of bulls carrying cytogenetic defects to the baseline values. With the exception of one case the test was able to identify bulls with cytogenetic defects. Therefore, we conclude that the assay could be incorporated into the laboratory routine where flow cytometry is applied for semen quality control.
authors.affiliation: Department of Animal Sciences and Animal Husbandry, Georgikon Faculty, University of Pannonia , Deák F. u. 16, H-8360 Keszthely , Hungary.
authors.forename: Szabolcs
authors.initials: S
authors.lastname: Nagy
authors.affiliation: Department of Animal Sciences and Animal Husbandry, Georgikon Faculty, University of Pannonia , Deák F. u. 16, H-8360 Keszthely , Hungary.
authors.forename: Péter J
authors.initials: PJ
authors.lastname: Polgár
authors.affiliation: Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki , Saari Unit, Saarentaus , Finland.
authors.forename: Magnus
authors.initials: M
authors.lastname: Andersson
authors.affiliation: Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen , Debrecen , Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Kovács
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D003582:Cytogenetics / Q000379:methods*; D004247:DNA / Q000235:genetics*; D005434:Flow Cytometry / Q000662:veterinary*; D008297:Male; D055101:Semen Analysis / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D004247:DNA
keywords: Cytogenetics; flow cytometry; sperm; translocation