title: Diversity of bats trypanosomes in hydroeletric area of Belo Monte in Brazilian Amazonia.
abstract: The Trypanosoma comprises flagellates able to infect many mammalian species and is transmitted by several groups of invertebrates. The order Chiroptera can be infected by the subgenera Herpetosoma, Schizotrypanum, Megatrypanum and Trypanozoon. In this study, we described the diversity of bats trypanosomes, inferring the phylogenetic relationships among the trypanosomes from bats caught Belo Monte Hydroeletric area (Brazilian Amazonia). Trypanosomes from bats were isolated by haemoculture, and the molecular phylogeny based on small subunit rDNA (SSU rDNA) and glycosomal-3-phosphate dehydrogenase (gGAPDH) gene sequences. Morphological characterization included light and scanning electron microscopy. A total of 157 bats were caught in the area belonging 6 Families (Emballonuridae, Furipteridae, Mormoopidae, Natalidae, Phyllostomidae and Vespertilionidae) and 34 species. The bat trypanosome prevalence, as evaluated through haemoculture, was 5,7%. Phylogenetic trees grouped the isolates in T. cruzi branch (TCI and TCbat lineage), T. cruzi marinkellei and Trypanosoma wauwau from Pteronotus parnellii. This is the first isolate from T. wauwau in Para state. The occurrence of T. cruzi in the ​​ Belo Monte Hydroeletric area (UHE Belo Monte) in Amazon/Brazil attentive to the risk of migration human population required for the works of the dam and new cities that grow in the vicinity of these businesses, but it is a zoonosis already known to the Amazon region, and the presence of unclassified Trypanosoma species, attend to the large parasitic biodiversity still unknown.
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Andréa P
authors.initials: AP
authors.lastname: da Costa
authors.affiliation: Universidade Federal da Integração Latino-Americana, Foz do Iguaçu, PR, Brazil.
authors.forename: Pablo Henrique
authors.initials: PH
authors.lastname: Nunes
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Universidade de São Paulo, São Paulo, SP, Brazil; Arcadis Logos, São Paulo, SP, Brazil.
authors.forename: Beatriz Helena Santos
authors.initials: BHS
authors.lastname: Leite
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Juliana Isabel G da S
authors.initials: JIGDS
authors.lastname: Ferreira
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, São Bernardo do Campo, SP, Brazil.
authors.forename: Renata
authors.initials: R
authors.lastname: Tonhosolo
authors.affiliation: Supervisão e vigilância em Saúde, Prefeitura Municipal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Adriana Ruckert
authors.initials: AR
authors.lastname: da Rosa
authors.affiliation: Departamento de Ecologia, Universidade Federal de Sergipe, São Cristovão, SE, Brazil.
authors.forename: Patricio Adriano
authors.initials: PA
authors.lastname: da Rocha
authors.affiliation: Universidade de Mogi das Cruzes, Mogi das Cruzes, SP, Brazil.
authors.forename: Caroline Cotrim
authors.initials: CC
authors.lastname: Aires
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Solange Maria
authors.initials: SM
authors.lastname: Gennari
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Universidade de São Paulo, São Paulo, SP, Brazil; Universidade de Santo Amaro, São Paulo, SP, Brazil. Electronic address: amarcili@usp.br.
authors.forename: Arlei
authors.initials: A
authors.lastname: Marcili
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D014355:Chagas Disease / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D002685:Chiroptera / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D017753:Ecosystem; D019143:Evolution, Molecular; D014644:Genetic Variation; D006801:Humans; D010802:Phylogeny; D011210:Power Plants; D015995:Prevalence; D017422:Sequence Analysis, DNA; D014349:Trypanosoma cruzi / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D015047:Zoonoses / Q000469:parasitology / Q000635:transmission
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal
keywords: Amazonia; Chiroptera; Phylogeny; Taxonomy; Trypanosoma