title: Effects of hydroxytyrosol on cardiovascular biomarkers in experimental diabetes mellitus.
abstract: The aim of this study was to assess the influence of hydroxytyrosol (HT) on cardiovascular biomarkers and morphometric parameters of the arterial wall in streptozotocin-diabetic rats. Seven groups of rats (N=10 per group) were studied for 2 months: nondiabetic rats (NDR), diabetic rats treated with saline (DR) and DR treated with HT (0.5, 1, 2.5, 5 and 10 mg kg-1day-1p.o.). DR had higher platelet aggregation values, higher thromboxane B2, plasma lipid peroxidation, 3-nitrotyrosine, oxidized LDL (oxLDL), myeloperoxidase, vascular cell adhesion molecule 1 (VCAM-1) and interleukin-1β (IL-1β) concentrations, and lower aortic 6-keto-prostaglandin F1αand nitric oxide production than NDR. Aortic wall area and smooth muscle cell count were also higher in DR than in NDR. HT significantly reduced both oxidative and nitrosative stress, oxLDL concentration, VCAM-1 and inflammatory mediators, platelet aggregation and thromboxane B2production. Morphometric values in the aortic wall were reduced to values near those in NDR. In conclusion, HT influenced the major biochemical processes leading to diabetic vasculopathy, and reduced cell proliferation in the vascular wall in this experimental model.
authors.affiliation: Departmento de Farmacología, Facultad de Medicina, Instituto de Investigación Biomédica (IBIMA), Universidad de Málaga.
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: López-Villodres
authors.affiliation: Departmento de Farmacología, Facultad de Medicina, Instituto de Investigación Biomédica (IBIMA), Universidad de Málaga.
authors.forename: Miriam
authors.initials: M
authors.lastname: Abdel-Karim
authors.affiliation: Departmento de Farmacología, Facultad de Medicina, Instituto de Investigación Biomédica (IBIMA), Universidad de Málaga.
authors.forename: José Pedro
authors.initials: JP
authors.lastname: De La Cruz
authors.affiliation: Departmento de Farmacología, Facultad de Medicina, Instituto de Investigación Biomédica (IBIMA), Universidad de Málaga.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Rodríguez-Pérez
authors.affiliation: Departmento de Farmacología, Facultad de Medicina, Instituto de Investigación Biomédica (IBIMA), Universidad de Málaga.
authors.forename: José Julio
authors.initials: JJ
authors.lastname: Reyes
authors.affiliation: Departmento de Farmacología, Facultad de Medicina, Instituto de Investigación Biomédica (IBIMA), Universidad de Málaga.
authors.forename: Rocío
authors.initials: R
authors.lastname: Guzmán-Moscoso
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Ctra Utrera km 1, Campus Universitario Pablo de Olavide, Edificio 46, Seville, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Rodriguez-Gutierrez
authors.affiliation: Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Ctra Utrera km 1, Campus Universitario Pablo de Olavide, Edificio 46, Seville, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Fernández-Bolaños
authors.affiliation: Departmento de Farmacología, Facultad de Medicina, Instituto de Investigación Biomédica (IBIMA), Universidad de Málaga. Electronic address: correa@uma.es.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: González-Correa
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000627:therapeutic use; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D001012:Aorta, Abdominal; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000150:complications / Q000276:immunology / Q000517:prevention & control*; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000178:diet therapy* / Q000378:metabolism / Q000473:pathology; D003925:Diabetic Angiopathies / Q000276:immunology / Q000517:prevention & control*; D058065:Diabetic Cardiomyopathies / Q000276:immunology / Q000517:prevention & control*; D019587:Dietary Supplements; D004730:Endothelium, Vascular / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D010626:Phenylethyl Alcohol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D010974:Platelet Aggregation; D017208:Rats, Wistar; D026361:Reactive Nitrogen Species / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism; D013311:Streptozocin
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D015415:Biomarkers; D018836:Inflammation Mediators; D008077:Lipoproteins, LDL; D026361:Reactive Nitrogen Species; C099252:oxidized low density lipoprotein; C005975:3,4-dihydroxyphenylethanol; D013311:Streptozocin; D010626:Phenylethyl Alcohol
keywords: Diabetes; Hydroxytyrosol; Inflammatory mediators; Oxidative stress; Platelets; Vasculopathy