title: Imaging Adenosine Triphosphate (ATP).
abstract: Adenosine triphosphate (ATP) is a universal mediator of metabolism and signaling across unicellular and multicellular species. There is a fundamental interdependence between the dynamics of ATP and the physiology that occurs inside and outside the cell. Characterizing and understanding ATP dynamics provide valuable mechanistic insight into processes that range from neurotransmission to the chemotaxis of immune cells. Therefore, we require the methodology to interrogate both temporal and spatial components of ATP dynamics from the subcellular to the organismal levels in live specimens. Over the last several decades, a number of molecular probes that are specific to ATP have been developed. These probes have been combined with imaging approaches, particularly optical microscopy, to enable qualitative and quantitative detection of this critical molecule. In this review, we survey current examples of technologies available for visualizing ATP in living cells, and identify areas where new tools and approaches are needed to expand our capabilities.
authors.affiliation: Department of Chemistry, Purdue University, 560 Oval Drive, Box 68, West Lafayette, Indiana 47907; and.
authors.forename: Megha
authors.initials: M
authors.lastname: Rajendran
authors.affiliation: Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, 76-211, Cambridge, Massachusetts 02139.
authors.forename: Eric
authors.initials: E
authors.lastname: Dane
authors.affiliation: Department of Chemistry, Purdue University, 560 Oval Drive, Box 68, West Lafayette, Indiana 47907; and.
authors.forename: Jason
authors.initials: J
authors.lastname: Conley
authors.affiliation: Department of Chemistry, Purdue University, 560 Oval Drive, Box 68, West Lafayette, Indiana 47907; and mtantama@purdue.edu.
authors.forename: Mathew
authors.initials: M
authors.lastname: Tantama
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry; D060807:Bioluminescence Resonance Energy Transfer Techniques; D031541:Fluorescence Resonance Energy Transfer; D008853:Microscopy; D057054:Molecular Imaging; D015335:Molecular Probes / Q000737:chemistry; D061848:Optical Imaging
chemical_list: D052157:Aptamers, Nucleotide; D015335:Molecular Probes; D000255:Adenosine Triphosphate