title: Characterization of Pv92, a Novel Merozoite Surface Protein of Plasmodium vivax.
abstract: The discovery and understanding of antigenic proteins are essential for development of a vaccine against malaria. In Plasmodium falciparum, Pf92 have been characterized as a merozoite surface protein, and this protein is expressed at the late schizont stage, but no study of Pv92, the orthologue of Pf92 in P. vivax, has been reported. Thus, the protein structure of Pv92 was analyzed, and the gene sequence was aligned with that of other Plasmodium spp. using bioinformatics tools. The recombinant Pv92 protein was expressed and purified using bacterial expression system and used for immunization of mice to gain the polyclonal antibody and for evaluation of antigenicity by protein array. Also, the antibody against Pv92 was used for subcellular analysis by immunofluorescence assay. The Pv92 protein has a signal peptide and a sexual stage s48/45 domain, and the cysteine residues at the N-terminal of Pv92 were completely conserved. The N-terminal of Pv92 was successfully expressed as soluble form using a bacterial expression system. The antibody raised against Pv92 recognized the parasites and completely merged with PvMSP1-19, indicating that Pv92 was localized on the merozoite surface. Evaluation of the human humoral immune response to Pv92 indicated moderate antigenicity, with 65% sensitivity and 95% specificity by protein array. Taken together, the merozoite surface localization and antigenicity of Pv92 implicate that it might be involved in attachment and invasion of a merozoite to a new host cell or immune evasion during invasion process.
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Seong-Kyun
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.|Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Jin-Hee
authors.initials: JH
authors.lastname: Han
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Myat Htut
authors.initials: MH
authors.lastname: Nyunt
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Fauzi
authors.initials: F
authors.lastname: Muh
authors.affiliation: Department of Clinical Microbiology and Applied Technology, Mahidol University, Bangkok, Thailand.
authors.forename: Patchanee
authors.initials: P
authors.lastname: Chootong
authors.affiliation: Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Kwon-Soo
authors.initials: KS
authors.lastname: Ha
authors.affiliation: Department of Physiology, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Park
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Seok-Ho
authors.initials: SH
authors.lastname: Hong
authors.affiliation: Department of Anatomy, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Jeong-Hyun
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Eun-Taek
authors.initials: ET
authors.lastname: Han
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D019295:Computational Biology; D005260:Female; D015870:Gene Expression; D006801:Humans; D016780:Malaria, Vivax / Q000175:diagnosis / Q000276:immunology; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology*; D052940:Merozoites / Q000737:chemistry; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000235:genetics; D010966:Plasmodium vivax / Q000737:chemistry / Q000235:genetics*; D015800:Protozoan Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D000913:Antibodies, Protozoan; D008565:Membrane Proteins; D015800:Protozoan Proteins; D011994:Recombinant Proteins
keywords: Plasmodium vivax; Pv92; antigenicity; merozoite surface protein