title: Production of 10S-hydroxy-8(E)-octadecenoic acid from oleic acid by whole recombinant Escherichia coli cells expressing 10S-dioxygenase from Nostoc punctiforme PCC 73102 with the aid of a chaperone.
abstract: OBJECTIVE
  To increase the production of 10S-hydroxy-8(E)-octadecenoic acid from oleic acid by whole recombinant Escherichia coli cells expressing Nostoc punctiforme 10S-dioxygenase with the aid of a chaperone.
  
  
  RESULTS
  The optimal conditions for 10S-hydroxy-8(E)-octadecenoic acid production by recombinant cells co-expressing chaperone plasmid were pH 9, 35 °C, 15 % (v/v) dimethyl sulfoxide, 40 g cells l-1, and 10 g oleic acid l-1. Under these conditions, recombinant cells co-expressing chaperone plasmid produced 7.2 g 10S-hydroxy-8(E)-octadecenoic acid l-1within 30 min, with a conversion yield of 72 % (w/w) and a volumetric productivity of 14.4 g l-1h-1.
  
  
  CONCLUSIONS
  The activity of recombinant cells expressing 10S-dioxygenase was increased by 200 % with the aid of a chaperone, demonstrating the first biotechnological production of 10S-hydroxy-8(E)-octadecenoic acid using recombinant cells expressing 10S-dioxygenase.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea.
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea.
authors.forename: Min-Ju
authors.initials: MJ
authors.lastname: Seo
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea.
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Shin
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea. deokkun@konkuk.ac.kr.
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D046937:Nostoc / Q000201:enzymology*; D019301:Oleic Acid / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism*
chemical_list: D013229:Stearic Acids; D019301:Oleic Acid; D049308:Dioxygenases
keywords: 10S-Dioxygenase; 10S-Hydroxy-8(E)-octadecenoic acid; Biotransformation; Chaperone; Nostoc punctiforme PCC 73102; Oleic acid