title: miR-361-5p inhibits hepatocellular carcinoma cell proliferation and invasion by targeting VEGFA.
abstract: MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. Here, we found that miR-361-5p is down-regulated in 135 patients with HCV-related hepatocellular carcinoma (HCC). Moreover, the expressions of miR-361-5p were highly correlated with VEGFA in these HCC patients. Further, CCK-8 proliferation assay indicated that miR-361-5p mimics inhibited the cell proliferation of HepG2 and SNU-398 HCC cells. Transwell assay showed that miR-361-5p mimics inhibited the invasion and migration of HepG2 and SNU-398 HCC cells. Luciferase assays revealed that miR-361-5p directly bound to the 3'untranslated region of VEGFA, and western blotting showed that miR-361-5p inhibited the expression of VEGFA. Generally, this study indicated that miR-361-5p is down-regulated in HCC and inhibits proliferation and invasion of HCC cell lines via VEGFA. In future, miR-361-5p will be a potential therapeutic agent for HCC.
authors.affiliation: Clinical Laboratory, Changzhou No.7 People's Hospital, Changzhou, China.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Cui
authors.affiliation: Clinical Laboratory, Changzhou No.7 People's Hospital, Changzhou, China.
authors.forename: Yuanguo
authors.initials: Y
authors.lastname: Li
authors.affiliation: Clinical Laboratory, Changzhou No.7 People's Hospital, Changzhou, China.
authors.forename: Keqing
authors.initials: K
authors.lastname: Xu
authors.affiliation: Clinical Laboratory, Changzhou No.7 People's Hospital, Changzhou, China.
authors.forename: Guijin
authors.initials: G
authors.lastname: Chen
authors.affiliation: Clinical Laboratory, Changzhou No.7 People's Hospital, Changzhou, China.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Lu
authors.affiliation: Clinical Laboratory, Changzhou No.7 People's Hospital, Changzhou, China.
authors.forename: Qiuqin
authors.initials: Q
authors.lastname: Duan
authors.affiliation: Clinical Laboratory, Changzhou No.7 People's Hospital, Changzhou, China.
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Kang
mesh_terms: D020413:3' Untranslated Regions; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D018390:Gene Targeting; D056945:Hep G2 Cells; D019698:Hepatitis C, Chronic / Q000150:complications; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000517:prevention & control; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors* / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C575475:MIRN361 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: HCV; Hepatocellular carcinoma; VEGFA; miR-361-5p