title: Hiding in the Shadows: CPOX Expression and 5-ALA Induced Fluorescence in Human Glioma Cells.
abstract: Protoporphyrin IX (PpIX) is widely used in photodynamic diagnosis. To date, the details of molecular mechanisms underlying PpIX accumulation in malignant cells after 5-ALA administration remain unclear. The fluorescence of PpIX was studied in human glioma cells. Several cell cultures were established from glioma tumor tissue to study the differences between fluorescence-positive and fluorescence-negative human glioma tumors. The cell cultures demonstrated fluorescence profiles similar to those of source tumor tissues, which allows us to use these cultures in experimental research. Dynamics of the rates of synthesis and degradation of fluorescent protoporphyrin IX was studied in the cultures obtained. In addition, the expression of CPOX, an enzyme involved in PpIX synthesis, was evaluated. mRNA levels of heme biosynthesis enzymes were analyzed, and PpIX fluorescence proved to correlate with the CPOX protein level, whereas no such correlation was observed at the mRNA level. Fluorescence intensity decreased at low levels of the enzyme, which indicates its critical role in PpIX fluorescence. Finally, the fluorescence intensity proved to correlate with the proliferative activity.
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Vavilov Str., 34/5, Moscow, Russian Federation, 119334.|Ltd Apto-pharm, Kolomensky Road, 13A, Moscow, Russia, 115446.
authors.forename: Nikolay
authors.initials: N
authors.lastname: Pustogarov
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Vavilov Str., 34/5, Moscow, Russian Federation, 119334.
authors.forename: Dmitriy
authors.initials: D
authors.lastname: Panteleev
authors.affiliation: Burdenko Neurosurgical Institute, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Goryaynov
authors.affiliation: General Physics Institute of Russian Academy of Sciences, National Research Nuclear University, Moscow, Russia.
authors.forename: Anastasia V
authors.initials: AV
authors.lastname: Ryabova
authors.affiliation: Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, 115487, Russia.
authors.forename: Ekaterina Y
authors.initials: EY
authors.lastname: Rybalkina
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Vavilov Str., 34/5, Moscow, Russian Federation, 119334.|Ltd Apto-pharm, Kolomensky Road, 13A, Moscow, Russia, 115446.
authors.forename: Alexander
authors.initials: A
authors.lastname: Revishchin
authors.affiliation: Burdenko Neurosurgical Institute, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Potapov
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Vavilov Str., 34/5, Moscow, Russian Federation, 119334. lkorochkin@mail.ru.|Ltd Apto-pharm, Kolomensky Road, 13A, Moscow, Russia, 115446. lkorochkin@mail.ru.
authors.forename: Galina
authors.initials: G
authors.lastname: Pavlova
mesh_terms: D000622:Aminolevulinic Acid / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D003304:Coproporphyrinogen Oxidase / Q000235:genetics / Q000378:metabolism*; D005453:Fluorescence; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011524:Protoporphyrins / Q000494:pharmacology*
chemical_list: D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX; D003304:Coproporphyrinogen Oxidase
keywords: 5-ALA; CPOX; Cell cultures; Glioma; PpIX