title: Soybean protein as a cost-effective lignin-blocking additive for the saccharification of sugarcane bagasse.
abstract: Addition of surfactants, polymers, and non-catalytic proteins can improve the enzymatic hydrolysis of lignocellulosic materials by blocking the exposed lignin surfaces, but involves extra expense. Here, soybean protein, one of the cheapest proteins available, was evaluated as an alternative additive for the enzymatic hydrolysis of pretreated sugarcane bagasse. The effect of the enzyme source was investigated using enzymatic cocktails from A. niger and T. reesei cultivated under solid-state, submerged, and sequential fermentation. The use of soybean protein led to approximately 2-fold increases in hydrolysis, relative to the control, for both A. niger and T. reesei enzymatic cocktails from solid-state fermentation. The effect was comparable to that of BSA. Moreover, the use of soybean protein and a 1:1 combination of A. niger and T. reesei enzymatic cocktails resulted in 54% higher glucose release, compared to the control. Soybean protein is a potential cost-effective additive for use in the biomass conversion process.
authors.affiliation: Embrapa Instrumentação, Rua XV de Novembro 1452, 13560-970 São Carlos, SP, Brazil; Graduate Program of Biotechnology, Federal University of São Carlos, 13565-905 Sao Carlos, SP, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Florencio
authors.affiliation: Graduate Program of Biotechnology, Federal University of São Carlos, 13565-905 Sao Carlos, SP, Brazil; Graduate Program of Chemical Engineering, Federal University of São Carlos, 13565-905 Sao Carlos, SP, Brazil.
authors.forename: Alberto C
authors.initials: AC
authors.lastname: Badino
authors.affiliation: Embrapa Instrumentação, Rua XV de Novembro 1452, 13560-970 São Carlos, SP, Brazil; Graduate Program of Biotechnology, Federal University of São Carlos, 13565-905 Sao Carlos, SP, Brazil; Graduate Program of Chemical Engineering, Federal University of São Carlos, 13565-905 Sao Carlos, SP, Brazil. Electronic address: cristiane.farinas@embrapa.br.
authors.forename: Cristiane S
authors.initials: CS
authors.lastname: Farinas
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D018533:Biomass; D001709:Biotechnology / Q000191:economics / Q000379:methods*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D003362:Cost-Benefit Analysis; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D031786:Saccharum / Q000737:chemistry / Q000378:metabolism*; D030262:Soybean Proteins / Q000191:economics / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology
chemical_list: D004798:Enzymes; D030262:Soybean Proteins; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D005947:Glucose
keywords: Adsorption; Lignin; Lignocellulosic biomass; Soybean protein; Sugarcane bagasse