title: The germin-like protein OsGLP2-1 enhances resistance to fungal blast and bacterial blight in rice.
abstract: This is the first report that GLP gene (OsGLP2-1) is involved in panicle blast and bacterial blight resistance in rice. In addition to its resistance to blast and bacterial blight, OsGLP2-1 has also been reported to co-localize with a QTLs for sheath blight resistance in rice. These suggest that the disease resistance provided by OsGLP2-1 is quantitative and broad spectrum. Its good resistance to these major diseases in rice makes it to be a promising target in rice breeding. Rice (Oryza sativa) blast caused by Magnaporthe oryzae and bacterial blight caused by Xanthomonas oryzae pv. oryzae are the two most destructive rice diseases worldwide. Germin-like protein (GLP) gene family is one of the important defense gene families which have been reported to be involved in disease resistance in plants. Although GLP proteins have been demonstrated to positively regulate leaf blast resistance in rice, their involvement in resistance to panicle blast and bacterial blight, has not been reported. In this study, we reported that one of the rice GLP genes, OsGLP2-1, was significantly induced by blast fungus. Overexpression of OsGLP2-1 quantitatively enhanced resistance to leaf blast, panicle blast and bacterial blight. The temporal and spatial expression analysis revealed that OsGLP2-1is highly expressed in leaves and panicles and sub-localized in the cell wall. Compared with empty vector transformed (control) plants, the OsGLP2-1 overexpressing plants exhibited higher levels of H2O2both before and after pathogen inoculation. Moreover, OsGLP2-1 was significantly induced by jasmonic acid (JA). Overexpression of OsGLP2-1 induced three well-characterized defense-related genes which are associated in JA-dependent pathway after pathogen infection. Higher endogenous level of JA was also identified in OsGLP2-1 overexpressing plants than in control plants both before and after pathogen inoculation. Together, these results suggest that OsGLP2-1 functions as a positive regulator to modulate disease resistance. Its good quantitative resistance to the two major diseases in rice makes it to be a promising target in rice breeding.
authors.affiliation: Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China.|Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Guangdong Key Laboratory of New Technology in Plant protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.
authors.forename: Jianyuan
authors.initials: J
authors.lastname: Yang
authors.affiliation: Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
authors.forename: Shijuan
authors.initials: S
authors.lastname: Yan
authors.affiliation: Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China.|Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.
authors.forename: Shaohong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China.|Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.
authors.forename: Junliang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Guangdong Key Laboratory of New Technology in Plant protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wang
authors.affiliation: Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China.|Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.
authors.forename: Tifeng
authors.initials: T
authors.lastname: Yang
authors.affiliation: Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China.|Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China.|Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.
authors.forename: Xingxue
authors.initials: X
authors.lastname: Mao
authors.affiliation: Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China.|Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Dong
authors.affiliation: Guangdong Key Laboratory of New Technology in Plant protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China. gzzhuxy@tom.com.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China. lbgz1009@163.com.|Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China. lbgz1009@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
mesh_terms: D003517:Cyclopentanes / Q000494:pharmacology; D060467:Disease Resistance / Q000187:drug effects / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D020082:Magnaporthe / Q000502:physiology; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D054883:Oxylipins / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014974:Xanthomonas / Q000502:physiology
chemical_list: D003517:Cyclopentanes; D006023:Glycoproteins; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C058814:germin; C011006:jasmonic acid; D006861:Hydrogen Peroxide
keywords: Bacterial blight; H2O2; Jasmonic acid; Oryza sativa; OsGLP2-1; Rice blast