title: Correlation Between Poor Prognosis and Lower TPPP Gene Expression in Hepatocellular Carcinoma.
abstract: Post-resection recurrence of hepatocellular carcinoma (HCC) tends to derive from multicentric origins, which indicates that the background liver microenvironment affects carcinogenesis.
  
  
  
  We obtained control liver samples [super normal (SN)] from 11 patients with secondary metastatic liver malignancies and used expression and methylation arrays to compare them with non-cancerous liver tissue from a patient with typical HCC with chronic hepatitis C (corresponding normal (CN)].
  
  
  
  The expression array showed that gene expression of tubulin polymerization-promoting protein (TPPP) was lower in CN compared with SN. The methylation array showed a greater TPPP methylation index for CN than for SN. Transcripts of TPPP differed significantly among SN (n=11), CN (n=179), and tumor tissue of HCC (n=179) (median of 116, 4.60, and 2.63, respectively, p<0.001). Multivariate analysis showed lower TPPP expression in tumor than in normal tissue (ratio <0.3, n=57) to independently predict poor overall survival (p=0.031).
  
  
  
  Significantly lower TPPP expression was found in HCC and CN tissue compared to SN and indicated poor prognosis.
authors.affiliation: Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan Department of Surgery, Aichi Gakuin University School of Dentistry, Nagoya, Japan.
authors.forename: Yoshikuni
authors.initials: Y
authors.lastname: Inokawa
authors.affiliation: Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan Department of Surgery, Aichi Gakuin University School of Dentistry, Nagoya, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Sonohara
authors.affiliation: Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Mitsuro
authors.initials: M
authors.lastname: Kanda
authors.affiliation: Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Hayashi
authors.affiliation: Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nishikawa
authors.affiliation: Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugimoto
authors.affiliation: Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kodera
authors.affiliation: Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Nagoya, Japan Department of Surgery, Aichi Gakuin University School of Dentistry, Nagoya, Japan snomoto@dpc.agu.ac.jp.
authors.forename: Shuji
authors.initials: S
authors.lastname: Nomoto
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000378:metabolism* / Q000401:mortality; D045744:Cell Line, Tumor; D019175:DNA Methylation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006526:Hepatitis C / Q000150:complications / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009362:Neoplasm Metastasis; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D009419:Nerve Tissue Proteins; C118136:TPPP protein, human
keywords: Background liver; TPPP/p25; hepatocellular carcinoma; methylation; multi-array analysis