title: Cell cycle proliferation decisions: the impact of single cell analyses.
abstract: Cell proliferation is a fundamental requirement for organismal development and homeostasis. The mammalian cell division cycle is tightly controlled to ensure complete and precise genome duplication and segregation of replicated chromosomes to daughter cells. The onset of DNA replication marks an irreversible commitment to cell division, and the accumulated efforts of many decades of molecular and cellular studies have probed this cellular decision, commonly called the restriction point. Despite a long-standing conceptual framework of the restriction point for progression through G1 phase into S phase or exit from G1 phase to quiescence (G0), recent technical advances in quantitative single cell analysis of mammalian cells have provided new insights. Significant intercellular heterogeneity revealed by single cell studies and the discovery of discrete subpopulations in proliferating cultures suggests the need for an even more nuanced understanding of cell proliferation decisions. In this review, we describe some of the recent developments in the cell cycle field made possible by quantitative single cell experimental approaches.
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, NC, USA.
authors.forename: Jacob P
authors.initials: JP
authors.lastname: Matson
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, NC, USA.|Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, NC, USA.
authors.forename: Jeanette G
authors.initials: JG
authors.lastname: Cook
mesh_terms: D000818:Animals; D002455:Cell Division; D049109:Cell Proliferation / Q000235:genetics*; D018844:Cyclin-Dependent Kinases / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D050684:E2F Transcription Factors / Q000235:genetics / Q000378:metabolism; D005057:Eukaryotic Cells / Q000166:cytology / Q000378:metabolism*; D016193:G1 Phase / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D010766:Phosphorylation; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism; D016196:S Phase / Q000235:genetics; D015398:Signal Transduction; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D050684:E2F Transcription Factors; D016160:Retinoblastoma Protein; D018844:Cyclin-Dependent Kinases
keywords: G0; G1; G2; biosensor; cell cycle; cell division; quiescence; restriction point; review; single cell