title: Micellar Electrokinetic Chromatography of Aminoglycosides.
abstract: The components of the aminoglycosides, e.g., gentamicin, sisomicin, netilmicin, kanamycin, amikacin, and tobramycin, and related impurities of these antibiotics can be separated by means of micellar electrokinetic chromatography (MEKC). Derivatization with o-phthaldialdehyde and thioglycolic acid is found to be appropriate for these antibiotics. The background electrolyte was composed of sodium tetraborate (100 mM), sodium deoxycholate (20 mM), and β-cyclodextrin (15 mM) having a pH value of 10.0. This method is valid for evaluation of gentamicin, kanamycin, and tobramycin. It has to be adopted for amikacin, paromomycin, neomycin, and netilmicin.
authors.affiliation: Institut für Pharmazie und Lebensmittelchemie, Universität Würzburg, Am Hubland, Würzburg, 97074, Germany. u.holzgrabe@pharmazie.uni-wuerzburg.de.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Holzgrabe
authors.affiliation: Institut für Pharmazie und Lebensmittelchemie, Universität Würzburg, Am Hubland, Würzburg, 97074, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Schmitt
authors.affiliation: Institut für Pharmazie und Lebensmittelchemie, Universität Würzburg, Am Hubland, Würzburg, 97074, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Wienen
mesh_terms: D000583:Amikacin / Q000737:chemistry / Q000302:isolation & purification; D000617:Aminoglycosides / Q000737:chemistry / Q000302:isolation & purification*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D007612:Kanamycin / Q000737:chemistry / Q000302:isolation & purification; D008823:Micelles; D009428:Netilmicin / Q000737:chemistry / Q000302:isolation & purification; D012853:Sisomicin / Q000737:chemistry / Q000302:isolation & purification; D014031:Tobramycin / Q000737:chemistry / Q000302:isolation & purification; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D008823:Micelles; D047392:beta-Cyclodextrins; D009428:Netilmicin; D007612:Kanamycin; D000583:Amikacin; D014031:Tobramycin; D012853:Sisomicin
keywords: Aminoglycosides; Background electrolyte; Bile salts; Cyclodextrins; Gentamicin; Kanamycin; MEKC; Tobramycin