title: Characterization of Pseudomonas aeruginosa phage K5 genome and identification of its receptor related genes.
abstract: Phage genomic information and the nature of host-phage interactions are important for phage applications. In this study, Pseudomonas aeruginosa phage K5 is characterized as a linear double-stranded genomic DNA molecule of 93,754 bp with identical 1182-bp direct terminal repeats. Comparative genomic analysis reveals that phage K5 is highly homologous to the "PaP1-like" phages. Thirteen mutants resistant to phage K5 are screened in a transposon mutant library. The disrupted genetic loci are identified as gene Y880_RS05480 encoding a putative O-antigen polymerase Wzy and gene wapH encoding a glycosyltransferase. The mutants are confirmed by the complementation experiment. The production of biofilm and the profile of lipopolysaccharide (LPS) are further analyzed in the Y880_RS05480 mutant. Our data indicate that LPS is the receptor of phage K5.
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
authors.forename: Lingyan
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Pan
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Cui
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
authors.forename: Qinghui
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yang
authors.affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D018441:Biofilms / Q000254:growth & development; D004251:DNA Transposable Elements; D004279:DNA, Viral / Q000235:genetics; D005816:Genetic Complementation Test; D016679:Genome, Viral; D016695:Glycosyltransferases / Q000235:genetics; D006602:Hexosyltransferases / Q000235:genetics; D054884:Host-Pathogen Interactions; D009154:Mutation; D019081:O Antigens / Q000737:chemistry / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D017105:Pseudomonas Phages / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000502:physiology / Q000821:virology*; D011991:Receptors, Virus / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004251:DNA Transposable Elements; D004279:DNA, Viral; D019081:O Antigens; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D011991:Receptors, Virus; D016695:Glycosyltransferases; D006602:Hexosyltransferases; C085652:O-antigen polymerase
keywords: Genome annotation; Phage K5; Phage receptor related genes; Pseudomonas aeruginosa