title: Human extraintestinal pathogenic Escherichia coli strains differ in prevalence of virulence factors, phylogroups, and bacteriocin determinants.
abstract: The study used a set of 407 human extraintestinal pathogenic E. coli strains (ExPEC) isolated from (1) skin and soft tissue infections, (2) respiratory infections, (3) intra-abdominal infections, and (4) genital smears. The set was tested for bacteriocin production, for prevalence of bacteriocin and virulence determinants, and for phylogenetic typing. Results obtained from the group of ExPEC strains were compared to data from our previously published analyses of 1283 fecal commensal E. coli strains.
  
  
  
  The frequency of bacteriocinogeny was significantly higher in the set of ExPEC strains (63.1 %), compared to fecal E. coli (54.2 %; p < 0.01). Microcin producers and microcin determinants dominated in ExPEC strains, while colicin producers and colicin determinants were more frequent in fecal E. coli (p < 0.01). Higher production of microcin M and lower production of microcin B17, colicin Ib, and Js was detected in the set of ExPEC strains. ExPEC strains had a significantly higher prevalence of phylogenetic group B2 (52.6 %) compared to fecal E. coli strains (38.3 %; p < 0.01).
  
  
  
  Human ExPEC strains were shown to differ from human fecal strains in a number of parameters including bacteriocin production, prevalence of several bacteriocin and virulence determinants, and prevalence of phylogenetic groups. Differences in these parameters were also identified within subgroups of ExPEC strains of diverse origin. While some microcin determinants (mM, mH47) were associated with virulent strains, other bacteriocin types (mB17, Ib, and Js) were associated with fecal flora.
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00, Brno, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Micenková
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00, Brno, Czech Republic.
authors.forename: Juraj
authors.initials: J
authors.lastname: Bosák
authors.affiliation: Department of Clinical Microbiology, Faculty Hospital Brno, Jihlavská 20, 625 00, Brno, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Vrba
authors.affiliation: Department of Clinical Microbiology, Faculty Hospital Brno, Jihlavská 20, 625 00, Brno, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Ševčíková
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Building A6, 625 00, Brno, Czech Republic. dsmajs@med.muni.cz.
authors.forename: David
authors.initials: D
authors.lastname: Šmajs
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001430:Bacteriocins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D003087:Colicins / Q000378:metabolism; D018153:Czech Republic; D004269:DNA, Bacterial / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology*; D000072282:Extraintestinal Pathogenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism* / Q000472:pathogenicity; D005243:Feces / Q000382:microbiology; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D015995:Prevalence; D060737:Reproductive Tract Infections / Q000382:microbiology; D012137:Respiratory System / Q000382:microbiology; D012867:Skin / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D001430:Bacteriocins; D003087:Colicins; D004269:DNA, Bacterial; D037521:Virulence Factors; C020411:microcin
keywords: Bacteriocinogeny; Colicin; Escherichia coli; ExPEC; Microcin; Virulence factor