title: miR-122 involved in the regulation of toll-like receptor signaling pathway after Vibrio anguillarum infection by targeting TLR14 in miiuy croaker.
abstract: Innate and acquired immune responses provide critical line in defense against pathogens. MicroRNAs (miRNAs) play crucial roles in regulate gene expression in inflammation and innate immunity. However, the main role of miRNAs are in a large part unclear in aquatic organisms. In this study, we found that miR-122 displayed dramatically declined expression profiles in Vibrio anguillarum challenged miiuy croaker. Meanwhile, we have explored TLR14 as a novel target gene of miR-122 involved in miiuy croakers inflammatory and immune response, which were further estimated through negative expression profiles in both Vibrio anguillarum challenged miiuy croaker and LPS exposure macrophages. Finally, in the dual-luciferase reporter assay presented mmi-miR-122 negatively regulated the 3'-UTR of wild-type in luciferase activity rather than the mutant one in HEK-293T cells. This result demonstrated that mmi-miR-122 modulated TLR14 expression by directly targeting TLR14-3'UTR. All of the present data suggested miR-122 was involved in TLR cascade could modulate Vibrio anguillarum infection in miiuy croakers.
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Junxia
authors.initials: J
authors.lastname: Cui
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chu
authors.affiliation: Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China. Electronic address: tianjunxu@163.com.
authors.forename: Tianjun
authors.initials: T
authors.lastname: Xu
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation; D007113:Immunity, Innate / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010473:Perciformes; D016415:Sequence Alignment / Q000662:veterinary; D017423:Sequence Analysis, RNA / Q000662:veterinary; D015398:Signal Transduction / Q000235:genetics*; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*
chemical_list: D008070:Lipopolysaccharides; D035683:MicroRNAs; D051193:Toll-Like Receptors
keywords: Immune response; TLR14; Toll-like receptor; miR-122; miRNA modulate