title: Glycome complexity of human seminal plasma high molecular mass components: Evaluation of the contribution of acid-soluble glycoproteins/mucins and extracellular vesicles.
abstract: This study was aimed at evaluation of the contribution of acid-soluble glycoproteins (ASG)/mucins and extracellular vesicles (EVs), yet unexplored components of human seminal plasma (hSP) to the complexity of its glycome. Gaining insight into the native presentation and distribution of glycans across hSP could help establish molecular environments supporting specific biological activities based on unique ligand capacities. Soluble and particulate fractions of hSP from healthy subjects were analyzed by gel filtration, electrophoresis, ion-exchange chromatography and a solid phase assay with immobilized charge-resolved glycospecies to test their reactivity with plant lectins, carbohydrate-binding antibodies and selected human lectins. Common O- and N-glycosylated species were detected on mixed or overlapped underlying protein scaffolds in both soluble and particulate fractions of hSP. Siaα2,6Gal and N-glycans were concentrated on EVs, whereas Siaα2,3Gal, T and Tn antigens were selectively associated with distinct glycospecies of ASG/mucins. Accessible ligands for the lectins, DC-SIGN and Siglec-9, were detected in all hSP components, but they preferentially bound to EVs glycospecies. Insight into the complexity of hSP glycans as recognition signals under normal physiological conditions could be of interest for regulation and possible modulation of its biological activity, as well as for biomarker potential related to male health.
authors.affiliation: University of Belgrade, Institute for the Application of Nuclear Energy, INEP, Banatska 31b, 11080 Zemun, Serbia. Electronic address: bojana@inep.co.rs.
authors.forename: Bojana
authors.initials: B
authors.lastname: Milutinovic
authors.affiliation: University of Belgrade, Institute for the Application of Nuclear Energy, INEP, Banatska 31b, 11080 Zemun, Serbia. Electronic address: ninoslavm@inep.co.rs.
authors.forename: Ninoslav
authors.initials: N
authors.lastname: Mitic
authors.affiliation: University of Belgrade, Institute for the Application of Nuclear Energy, INEP, Banatska 31b, 11080 Zemun, Serbia. Electronic address: jelena_ron@yahoo.com.
authors.forename: Jelena
authors.initials: J
authors.lastname: Roncevic
authors.affiliation: University of Belgrade, Institute for the Application of Nuclear Energy, INEP, Banatska 31b, 11080 Zemun, Serbia. Electronic address: sanjagoc@inep.co.rs.
authors.forename: Sanja
authors.initials: S
authors.lastname: Goc
authors.affiliation: University of Belgrade, Institute for the Application of Nuclear Energy, INEP, Banatska 31b, 11080 Zemun, Serbia. Electronic address: miraj@inep.co.rs.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Jankovic
mesh_terms: D015703:Antigens, CD / Q000737:chemistry; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry; D015415:Biomarkers / Q000737:chemistry; D019921:Biotinylation; D015815:Cell Adhesion Molecules / Q000737:chemistry; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry; D008024:Ligands; D008297:Male; D009077:Mucins / Q000737:chemistry*; D010472:Perchlorates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D012661:Semen / Q000737:chemistry*; D063265:Sialic Acid Binding Immunoglobulin-like Lectins / Q000737:chemistry; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015703:Antigens, CD; D015295:Antigens, Tumor-Associated, Carbohydrate; D015415:Biomarkers; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D037181:Lectins, C-Type; D008024:Ligands; D009077:Mucins; D010472:Perchlorates; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C412227:SIGLEC9 protein, human; C040286:Tn antigen; C576518:Perchloric Acid
keywords: Extracellular vesicles; Glycome; Mucins; Seminal plasma