title: Structure and cytotoxic activity of ulvan extracted from green seaweed Ulva lactuca.
abstract: The structure of an ulvan obtained by water extraction from green seaweed Ulva lactuca was elucidated by using IR, NMR, SEC-MALL and ESIMS methods. The ulvan was also evaluated for its cytotoxic effects on three human cancer cell lines. The results showed that the ulvan was composed of rhamnose, galactose, xylose, manose, glucose (with a mole ratio of Rha: Gal: Xyl: Man: Glu equal to 1: 0.03: 0.07: 0.01: 0.06), uronic acid (21.5%) and sulfate content (18.9%) with a molecular weight of 347000. This ulvan mainly consists of disaccharide [→4)-β-d-GlcA-(1→4)-α-l-Rha3S-(1→] and other minor disaccharide β-GlcA-(1→2)-α-Xyl and β-GlcA-(→2)-α-Rha. The ulvan showed a significant cytotoxic activity against hepatocellular carcinoma (IC5029.67±2.87μg/ml), human breast cancer (IC5025.09±1.36μg/ml), and cervical cancer (IC5036.33±3.84μg/ml).
authors.affiliation: Institute of Chemistry, Vietnam Academy of Science and Technology, Vietnam. Electronic address: thuyttt@ich.vast.vn.
authors.forename: Thi Thu Thuy
authors.initials: TT
authors.lastname: Thanh
authors.affiliation: Institute of Chemistry, Vietnam Academy of Science and Technology, Vietnam.
authors.forename: Thi Minh Thu
authors.initials: TM
authors.lastname: Quach
authors.affiliation: Hanoi Metropolitan University, Vietnam.
authors.forename: Thi Nu
authors.initials: TN
authors.lastname: Nguyen
authors.affiliation: Institute of Chemistry, Vietnam Academy of Science and Technology, Vietnam.
authors.forename: Dang
authors.initials: D
authors.lastname: Vu Luong
authors.affiliation: Nhatrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Vietnam.
authors.forename: Minh Ly
authors.initials: ML
authors.lastname: Bui
authors.affiliation: Nhatrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Vietnam.
authors.forename: Thi Thanh Van
authors.initials: TT
authors.lastname: Tran
mesh_terms: D002240:Carbohydrate Sequence; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D008297:Male; D009369:Neoplasms / Q000188:drug therapy*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D044445:Ulva / Q000737:chemistry*
chemical_list: D003603:Cytotoxins; D011134:Polysaccharides; C571831:ulvan
keywords: Cytotoxic activity; Green seaweed; Ulva lactuca; Ulvan