title: NSrp70 is significant for embryonic growth and development, being a crucial factor for gastrulation and mesoderm induction.
abstract: NSrp70 (nuclear speckle-related protein 70), a recently discovered protein and it belongs to the serine/arginine (SR) rich related protein family. NSrp70 is recognized as an important splicing factor comprising RNA recognition motif (RRM) and arginine/serine (RS)-like regions at the N- and C-terminus respectively, along with two coiled coil domains at each terminus. However, other functions of NSrp70 remain unelucidated. In this study, we investigated the role of NSrp70 in Xenopus embryogenesis and found that its maternal expression plays a critical role in embryonic development. Knockdown of NSrp70 resulted in dramatic reduction in the length of developing tadpoles and mild to severe malformation in Xenopus embryos. In addition, knockdown of NSrp70 resulted in an extremely short axis by blocking gastrulation and convergent extension. Further, animal cap assays along with activin A treatment revealed that NSrp70 is an essential factor for dorsal mesoderm induction as knockdown of NSrp70 caused a dramatic down-regulation of dorsal mesoderm specific genes and its loss significantly shortened the elongation region of animal caps. In conclusion, NSrp70 is crucial for early embryonic development, influencing gastrulation and mesoderm induction.
authors.affiliation: ABRC, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, South Korea.
authors.forename: Soo-Ho
authors.initials: SH
authors.lastname: Lee
authors.affiliation: ABRC, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, South Korea.
authors.forename: Chowon
authors.initials: C
authors.lastname: Kim
authors.affiliation: ABRC, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, South Korea.
authors.forename: Hyun-Kyung
authors.initials: HK
authors.lastname: Lee
authors.affiliation: ABRC, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, South Korea.
authors.forename: Yoo-Kyung
authors.initials: YK
authors.lastname: Kim
authors.affiliation: ABRC, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, South Korea.
authors.forename: Tayaba
authors.initials: T
authors.lastname: Ismail
authors.affiliation: ABRC, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, South Korea.
authors.forename: Youngeun
authors.initials: Y
authors.lastname: Jeong
authors.affiliation: ABRC, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, South Korea.
authors.forename: Kyungyeon
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Anatomy, College of Medicine, Kyungpook National University, Daegu 41944, South Korea.
authors.forename: Mae-Ja
authors.initials: MJ
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, School of Medicine, Wonkwang University, Iksan 54538, South Korea.
authors.forename: Do-Sim
authors.initials: DS
authors.lastname: Park
authors.affiliation: ABRC, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, South Korea. Electronic address: leeh@knu.ac.kr.
authors.forename: Hyun-Shik
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019521:Body Patterning / Q000235:genetics; D005260:Female; D054262:Gastrulation / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D017403:In Situ Hybridization; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008648:Mesoderm / Q000196:embryology / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics* / Q000254:growth & development
chemical_list: C000620295:NSrp70 protein, Xenopus; D009687:Nuclear Proteins; D029867:Xenopus Proteins
keywords: Convergent extension; Embryogenesis; Gastrulation; NSrp70; SRSF1