title: Lipid Regulation Effects of Raw and Processed Notoginseng Radix Et Rhizome on Steatotic Hepatocyte L02 Cell.
abstract: Introduction. Raw and processed Notoginseng Radix Et Rhizome (NRR) have been widely used in treatment of metabolic syndromes and related disease, including nonalcoholic fatty liver disease (NAFLD). This study was designed to investigate lipid regulation effects of raw and processed NRR in steatotic L02 cell. Materials and Methods. Steatotic L02 cells were obtained after being cultured with 5% fat emulsion-10% FBS-RPMI 1640 medium for 48 h. Contents of total cholesterol (TC), triglyceride (TG), free fatty acid (FFA), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in steatotic L02 cells were evaluated after treatment. Furthermore, the lipid metabolism regulation mechanism of Panax notoginseng saponins (PNS) and its monomers were evaluated by detecting the expressions of hydroxymethyl glutaric acyl coenzyme A reductase (HMG-CoAR), sterol regulating element binding protein-2 (SREBP-2), and cholesterol 7α-hydroxylase (CYP7α). Results. TG and TC contents were doubled in model group compared to those in normal L02 cells group. Raw NRR and NRR heated with sand (NRR-B) showed much remarkable lipid-lowering effects in steatotic L02 cells. PNS, notoginsenoside R1, ginsenoside Rg1, and ginsenoside Rb1 displayed the best TG and TC regulation activity, which could significantly reduce contents of SREBP-2 and HMG-CoAR and increase the content of CYP7α. Conclusions. Our results may support the fact that both raw NRR and NRR-B might have more satisfactory effects in the treatment of NAFLD.
authors.affiliation: Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan 650500, China.
authors.forename: Zhu
authors.identifier: 0000-0002-6162-885X
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan 650500, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.affiliation: Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan 650500, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Yang
authors.affiliation: Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan 650500, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan 650500, China.
authors.forename: Xiaojian
authors.identifier: 0000-0003-0141-4347
authors.initials: X
authors.lastname: Mao
authors.affiliation: Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan 650500, China.
authors.forename: Jie
authors.identifier: 0000-0001-8100-8896
authors.initials: J
authors.lastname: Yu
mesh_terms: D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004655:Emulsions; D005234:Fatty Liver / Q000473:pathology*; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000032:analysis; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000494:pharmacology; D051782:Sterol Regulatory Element Binding Protein 2 / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D004655:Emulsions; D008055:Lipids; C519374:Panax notoginseng extract; D012503:Saponins; D051782:Sterol Regulatory Element Binding Protein 2; D014315:Triterpenes