title: Primary Investigation for the Mechanism of Biatractylolide from Atractylodis Macrocephalae Rhizoma as an Acetylcholinesterase Inhibitor.
abstract: Biatractylolide was isolated from ethyl acetate extract of dried Atractylodis Macrocephalae Rhizoma root by multistep chromatographic processing. Structure of biatractylolide was confirmed by (1)H-NMR and (13)C-NMR. The IC50 on acetylcholinesterase (AChE) activity was 6.5458 μg/mL when the control IC50 value of huperzine A was 0.0192 μg/mL. Molecular Docking Software (MOE) was used to discover molecular sites of action between biatractylolide and AChE protein by regular molecular docking approaches. Moreover, biatractylolide downregulated the expression of AChE of MEF and 293T cells in a dose-dependent manner. These results demonstrated that the molecular mechanisms of inhibitory activities of biatractylolide on AChE are not only through binding to AChE, but also via reducing AChE expression by inhibiting the activity of GSK3β.
authors.affiliation: Department of Pharmacy, School of Medicine, Hunan Normal University, Changsha, Hunan 410013, China.
authors.forename: Yong-Chao
authors.initials: YC
authors.lastname: Xie
authors.affiliation: The First Affiliated Hospital, Hunan Normal University, 61 West Jiefang Road, Changsha, Hunan 410005, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ning
authors.affiliation: Department of Pharmacy, School of Medicine, Hunan Normal University, Changsha, Hunan 410013, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Pharmacy, School of Medicine, Hunan Normal University, Changsha, Hunan 410013, China.
authors.forename: Dan-Ning
authors.initials: DN
authors.lastname: Li
authors.affiliation: Department of Pharmacy, School of Medicine, Hunan Normal University, Changsha, Hunan 410013, China.
authors.forename: Xing
authors.identifier: 0000-0003-2231-3705
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Pharmacy, School of Medicine, Hunan Normal University, Changsha, Hunan 410013, China.
authors.forename: Xiao-Ping
authors.identifier: 0000-0003-1952-7227
authors.initials: XP
authors.lastname: Yang