title: Impacts of nitrate and electron donor on perchlorate reduction and microbial community composition in a biologically activated carbon reactor.
abstract: The sensitivity of perchlorate reduction and microbial composition to varied nitrate and acetate loadings was studied in a biologically activated carbon reactor with perchlorate loading and empty bed contact time fixed at 5 mg/L and 226 min, respectively. In stage 1, the sole electron acceptor ClO4-realized complete removal with ≥21.95 mg C/L of acetate supply. As nitrate loading gradually increased to 5 mg/L (stage 2), perchlorate reduction was slightly promoted and both ClO4-and NO3-were completely removed at an acetate loading of 29.7 mg C/L. When nitrate loading continued increasing to 10-60 mg/L (stage 3), perchlorate reduction converted to be inhibited, along with nondetectable NO3-and approximately exhausted DOC in effluent. When acetate loading increased to 43.9 mg C/L in stage 4, both ClO4-and NO3-were again removed, though lags still existed in perchlorate reduction. β-Proteobacteria accounted for about 60%, 55%, 58%, 61% and 12% in samples from the base and top of the filter in stage 1 and those from the base, middle and top in stage 4, respectively. These findings implied that ratio of NO3-to ClO4-loadings and acetate loading were two key factors impacting ClO4-reduction and microbial structure along the filter.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, 200092, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, 200092, China. Electronic address: minw@tongji.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, 200092, China.
authors.forename: Naiyun
authors.initials: N
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, 200092, China.
authors.forename: Wenhai
authors.initials: W
authors.lastname: Chu
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, 200092, China.
authors.forename: Shuaifeng
authors.initials: S
authors.lastname: Wang
mesh_terms: D000085:Acetates / Q000737:chemistry; D019149:Bioreactors / Q000382:microbiology*; D002606:Charcoal / Q000737:chemistry*; D004583:Electrons; D009566:Nitrates / Q000737:chemistry*; D010084:Oxidation-Reduction; D010472:Perchlorates / Q000032:analysis* / Q000737:chemistry; D020560:Proteobacteria / Q000254:growth & development; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry; D018508:Water Purification / Q000379:methods*
chemical_list: D000085:Acetates; D009566:Nitrates; D010472:Perchlorates; D014874:Water Pollutants, Chemical; D002606:Charcoal; C494474:perchlorate
keywords: Biologically activated carbon (BAC); Electron donor; Microbial composition; Nitrate; Perchlorate