title: Comparison of human papillomavirus type 16 replication in tonsil and foreskin epithelia.
abstract: Human papillomavirus (HPV) is well recognized as a causative agent for anogenital and oropharyngeal cancers, however, the biology of HPV infection at different mucosal locations, specifically the oral cavity, is not well understood. Importantly, it has yet to be determined if oral tissues are permissive for HPV infection and replication. We investigated for the first time the titers, infectivity, and maturation of HPV16 in oral epithelial versus genital epithelial tissue. We show that infectious HPV16 virions can be produced in oral tissue. This demonstrates, for the first time, that infectious virus could be spread via the oral cavity. HPV16 derived from oral tissue utilize a tissue-spanning redox gradient that facilitates the maturation of virions over time. Maturation is manifested by virion stability and increased susceptibility to neutralization with anti-HPV16 L1 antibodies. However, susceptibility to neutralization by anti-HPV16 L2 specific antibodies decreases during the maturation of HPV16 virions in oral tissue.
authors.affiliation: Department of Pediatrics, Division of Allergy and Immunology, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY 11030, USA.
authors.forename: Mohd
authors.initials: M
authors.lastname: Israr
authors.affiliation: Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Biryukov
authors.affiliation: Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, PA 19104, USA.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Ryndock
authors.affiliation: Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA.
authors.forename: Samina
authors.initials: S
authors.lastname: Alam
authors.affiliation: Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA. Electronic address: cmm10@psu.edu.
authors.forename: Craig
authors.initials: C
authors.lastname: Meyers
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D004848:Epithelium / Q000821:virology*; D052816:Foreskin / Q000821:virology*; D052162:Human papillomavirus 16 / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000821:virology; D008297:Male; D051379:Mice; D009500:Neutralization Tests; D010084:Oxidation-Reduction; D014066:Palatine Tonsil / Q000821:virology*; D030361:Papillomavirus Infections / Q000150:complications / Q000276:immunology / Q000821:virology*; D014771:Virion; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D036022:Capsid Proteins
keywords: HPV capsid proteins; Head and neck cancer; Human papillomavirus; Oropharyngeal cancer