title: Immunogenicity and protective immunity against otitis media caused by pneumococcus in mice of Hib conjugate vaccine with PsaA protein carrier.
abstract: This study evaluated the immunogenicity and protective immunity of a Hemophilus influenzae b (Hib) polysaccharide conjugate vaccine with the pneumococcal surface adhesin A (PsaA) protein carrier in young mice. The Hib polysaccharide was conjugated with the rPsaA protein carrier, which was produced using recombinant DNA technology. A total of 15 young mice aged 3 weeks to 5 weeks were immunized with the conjugate vaccine, and another 15 young mice of the same age were immunized with the licensed Hib-tetanus toxoid (TT) vaccine. Furthermore, the third group of 15 young mice was inoculated with phosphate buffer saline as control. The immunized mice were inoculated with pneumococcus in the middle ear. Results showed that IgG antibody responses against both the PsaA protein and Hib polysaccharide were observed in the Hib-PsaA group. However, no statistical difference was observed in the titer of IgG against the Hib polysaccharide between Hib-PsaA and Hib-TT groups. The elimination rate of pneumococcus and the inflammation of the middle ear showed the effectiveness of protective immunity against otitis media caused by pneumococcus. Our results suggest that the Hib polysaccharide can be successfully conjugated with rPsaA via amide condensation. This new Hib-PsaA conjugate vaccine can induce both anti-PsaA and anti-Hib immune responses in young mice and elicit effective protection against acute otitis media caused by pneumococcus.
authors.affiliation: Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China. chenzeyuent@163.com.|Shanghai Clinical Medical Center of Hearing Medicine, Shanghai, 200031, China. chenzeyuent@163.com.
authors.forename: Zeyu
authors.initials: Z
authors.lastname: Chen
authors.affiliation: The Laboratory of Bacterial Vaccine, Wuhan Institute of Biological Products, Wuhan, 430207, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Guo
authors.affiliation: Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China.|Shanghai Clinical Medical Center of Hearing Medicine, Shanghai, 200031, China.
authors.forename: Jianghong
authors.initials: J
authors.lastname: Xu
authors.affiliation: Dingtai-Haigui Biotechnology Co. Ltd., Gu'an, 065500, China.
authors.forename: Chuangjun
authors.initials: C
authors.lastname: Qiu
mesh_terms: D000818:Animals; D016667:Bacterial Capsules; D001426:Bacterial Proteins / Q000737:chemistry*; D004432:Ear, Middle / Q000473:pathology; D005260:Female; D018073:Haemophilus Vaccines / Q000627:therapeutic use*; D019741:Haemophilus influenzae type b; D051379:Mice; D008807:Mice, Inbred BALB C; D010033:Otitis Media / Q000382:microbiology / Q000517:prevention & control*; D011008:Pneumococcal Infections / Q000517:prevention & control*; D013296:Streptococcus pneumoniae; D013745:Tetanus Toxoid / Q000627:therapeutic use; D018074:Vaccines, Conjugate / Q000627:therapeutic use
chemical_list: D001426:Bacterial Proteins; D018073:Haemophilus Vaccines; C055753:Haemophilus influenza type b polysaccharide vaccine-tetanus toxin conjugate; C044237:Haemophilus influenzae type b polysaccharide vaccine; D013745:Tetanus Toxoid; D018074:Vaccines, Conjugate; C051592:pneumococcal surface protein A
keywords: Hemophilus influenzae b; conjugate vaccine; immunogenicity; otitis media; pneumococcal surface adhesin A