title: Uterine focal adhesions are retained at implantation after rat ovarian hyperstimulation.
abstract: Controlled ovarian hyperstimulation is an essential component of IVF techniques to ensure proliferation and development of multiple ovarian follicles, but the effects of these hormones on the endometrium are largely unknown. During normal pregnancy in rats, there are significant changes in the basal plasma membrane of uterine epithelial cells (UECs) at the time of receptivity, including loss of focal adhesions. This enables the UECs to be removed from the implantation chamber surrounding the blastocyst, thus allowing invasion into the underlying stroma. This study investigated the influence of ovarian hyperstimulation (OH) on the basal plasma membrane of UECs during early pregnancy in the rat. Immunofluorescence results demonstrate the presence of paxillin, talin, integrin β1 and phosphorylated FAK (Y397FAK) in the basal portion of UECs at the time of implantation in OH pregnancy. TEM analysis demonstrated a flattened basal lamina and the presence of focal adhesions on the basal surface at this time in OH pregnancy. Significantly low full-length paxillin, high paxillin δ and integrin β1 were seen at the time of implantation in OH compared with those in normal pregnancy. The increase in paxillin δ suggests that these cells are less mobile, whereas the increase in integrin β1 and Y397FAK suggests the retention of a stable FA complex. Taken together with the increase in morphological focal adhesions, this represents a cell type that is stable and less easily removed for blastocyst implantation. This may be one mechanism explaining lower implantation rates after fresh embryo transfers compared with frozen cycles.
authors.affiliation: School of Medical Sciences (Anatomy and Histology)The University of Sydney, Sydney, New South Wales, Australia laural@anatomy.usyd.edu.au.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Lindsay
authors.affiliation: School of Medical Sciences (Anatomy and Histology)The University of Sydney, Sydney, New South Wales, Australia.
authors.forename: Samson N
authors.initials: SN
authors.lastname: Dowland
authors.affiliation: School of Medical Sciences (Anatomy and Histology)The University of Sydney, Sydney, New South Wales, Australia.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Murphy
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D010064:Embryo Implantation; D005260:Female; D022001:Focal Adhesions / Q000378:metabolism / Q000473:pathology*; D016471:Ovarian Hyperstimulation Syndrome / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D014599:Uterus / Q000378:metabolism / Q000473:pathology*