title: Multidimensional NMR approaches towards highly resolved, sensitive and high-throughput quantitative metabolomics.
abstract: Multi-dimensional NMR is an appealing approach for dealing with the challenging complexity of biological samples in metabolomics. This article describes how spectroscopists have recently challenged their imagination in order to make 2D NMR a powerful tool for quantitative metabolomics, based on innovative pulse sequences combined with meticulous analytical chemistry approaches. Clever time-saving strategies have also been explored to make 2D NMR a high-throughput tool for metabolomics, relying on alternative data acquisition schemes such as ultrafast NMR. Currently, much work is aimed at drastically boosting the NMR sensitivity thanks to hyperpolarisation techniques, which have been used in combination with fast acquisition methods and could greatly expand the application potential of NMR metabolomics.
authors.affiliation: CEISAM, UMR6230, Université de Nantes, BP 92208, 2 rue de la Houssinière, 44322 Nantes, France; LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), 44307 Nantes, France.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Marchand
authors.affiliation: CEISAM, UMR6230, Université de Nantes, BP 92208, 2 rue de la Houssinière, 44322 Nantes, France; Spectromaîtrise, CAPACITÉS SAS, 26 Bd Vincent Gâche, 44200 Nantes, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Martineau
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), 44307 Nantes, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Guitton
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), 44307 Nantes, France.
authors.forename: Gaud
authors.initials: G
authors.lastname: Dervilly-Pinel
authors.affiliation: CEISAM, UMR6230, Université de Nantes, BP 92208, 2 rue de la Houssinière, 44322 Nantes, France; Institut Universitaire de France, 1 rue Descartes, 75005 Paris Cedex 5, France. Electronic address: patrick.giraudeau@univ-nantes.fr.
authors.forename: Patrick
authors.initials: P
authors.lastname: Giraudeau
mesh_terms: D000818:Animals; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*