title: Altered Expression of PRKX, WNT3 and WNT16 in Human Nodular Basal Cell Carcinoma.
abstract: Nodular and superficial are the most common subtypes of basal cell carcinoma (BCC). Signaling pathways such as Hedgehog (HH) and Wingless (WNT) signaling are associated with BCC phenotypic variation. The aim of the study was to evaluate of the expression profiles of 84 genes related to the WNT and HH signaling pathways in patients with nodular and superficial BCC.
  
  
  
  A total of 58 BCCs and 13 samples of normal skin were evaluated by quantitative real-time polymerase chain reaction (qPCR) to detect the gene-expression profile.
  
  
  
  qPCR array showed segregation in BCC subtypes compared to healthy skin. PRKX, WNT3 and WNT16 were significantly (p<0.05) altered: PRKX was up-regulated, and WNT3 and WNT16 were down-regulated in nodular BCC.
  
  
  
  PRKX, WNT3 and WNT16 genes, belonging to the WNT signaling pathway, are involved in the tumorigenic process of nodular BCC.
authors.affiliation: Program of Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, DF, Brazil Program of Molecular Pathology, University of Brasília, Brasília, DF, Brazil.
authors.forename: Natalia Gurgel
authors.initials: NG
authors.lastname: DO Carmo
authors.affiliation: Domingos A. Boldrini Center for Hematological Investigation, Campinas, SP, Brazil.
authors.forename: Luis Henrique Toshihiro
authors.initials: LH
authors.lastname: Sakamoto
authors.affiliation: Program of Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, DF, Brazil.
authors.forename: Robert
authors.initials: R
authors.lastname: Pogue
authors.affiliation: Program of Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, DF, Brazil.
authors.forename: Cintia
authors.initials: C
authors.lastname: DO Couto Mascarenhas
authors.affiliation: Dermatology Service, Asa Norte Regional Hospital, Brasília, DF, Brazil.
authors.forename: Simone Karst
authors.initials: SK
authors.lastname: Passos
authors.affiliation: Program of Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, DF, Brazil Program of Molecular Pathology, University of Brasília, Brasília, DF, Brazil.
authors.forename: Maria Sueli Soares
authors.initials: MS
authors.lastname: Felipe
authors.affiliation: Program of Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, DF, Brazil rosangelavand@gmail.com.
authors.forename: Rosângela Vieira
authors.initials: RV
authors.lastname: DE Andrade
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002280:Basal Cell Carcinoma / Q000378:metabolism*; D049109:Cell Proliferation; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D012867:Skin / Q000378:metabolism; D012878:Skin Neoplasms / Q000378:metabolism*; D015854:Up-Regulation; D051153:Wnt Proteins / Q000378:metabolism*; D060508:Wnt3 Protein / Q000378:metabolism*
chemical_list: C417262:WNT16 protein, human; C555507:WNT3 protein, human; D051153:Wnt Proteins; D060508:Wnt3 Protein; C109450:PRKX protein, human; D017346:Protein Serine-Threonine Kinases
keywords: Basal cell carcinoma; Hedgehog; WNT; differential gene expression