title: HELLP Syndrome.
abstract: The hemolysis, elevated liver enzymes, and low platelet count (HELLP) syndrome is frequently observed in mothers whose offspring have long-chain fatty acid oxidation defects. We previously found that fatty acid oxidation is compromised not only in these inborn errors of metabolism but also in human umbilical vein endothelial cells (HUVECs) from all pregnancies complicated by the HELLP syndrome. Sirtuins are oxidized nicotinamide adenine dinucleotide (NAD+)dependent deacetylases linked to the metabolic status of the cell. SIRT 4 is known to have regulatory functions in fatty acid oxidation. The HELLP syndrome is often associated with short-term hypoxia. We studied sirtuins (SIRT 1, SIRT 3, and SIRT 4) in HUVECs from pregnancies complicated by the HELLP syndrome and uncomplicated pregnancies exposed to hypoxia (n = 7 controls, 7 HELLP; 0, 10, 60, or 120 minutes of 2% O2). Protein levels of SIRT 4 were significantly higher in HUVECs from HELLP compared to control after 60 and 120 minutes of hypoxia. The NAD+levels increased in a time-dependent manner.
authors.affiliation: 1 Department of Paediatric Kidney, Liver and Metabolic Diseases, Hannover Medical School, Hannover, Germany.
authors.forename: Mareike
authors.initials: M
authors.lastname: Sandvoß
authors.affiliation: 1 Department of Paediatric Kidney, Liver and Metabolic Diseases, Hannover Medical School, Hannover, Germany.
authors.forename: Arne Björn
authors.initials: AB
authors.lastname: Potthast
authors.affiliation: 2 Department of Obstetrics and Gynecology, Hannover Medical School, Hannover, Germany.
authors.forename: Frauke
authors.initials: F
authors.lastname: von Versen-Höynck
authors.affiliation: 1 Department of Paediatric Kidney, Liver and Metabolic Diseases, Hannover Medical School, Hannover, Germany.
authors.forename: Anibh Martin
authors.initials: AM
authors.lastname: Das
mesh_terms: D000328:Adult; D005260:Female; D005865:Gestational Age; D017359:HELLP Syndrome / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D011247:Pregnancy; D056564:Sirtuin 1 / Q000378:metabolism; D056566:Sirtuin 3 / Q000378:metabolism; D037761:Sirtuins / Q000378:metabolism*
chemical_list: D024101:Mitochondrial Proteins; C447939:SIRT1 protein, human; C466093:SIRT3 protein, human; C525357:SIRT4 protein, human; D056564:Sirtuin 1; D056566:Sirtuin 3; D037761:Sirtuins
keywords: HELLP syndrome; HUVECs; SIRT 4; fatty acid oxidation; hypoxia