title: LINC00312 inhibits the migration and invasion of bladder cancer cells by targeting miR-197-3p.
abstract: To investigate the influence of the long non-coding RNA LINC00312 on bladder cancer (BC) cell invasion and metastasis by targeting miR-197-3p. BC and corresponding adjacent tissues were collected. LINC00312 and miR-197-3p were measured, and their correlation was detected through quantitative real-time PCR (qRT-PCR). BC cell line T24 was transfected and grouped (five groups) according to different transfection conditions. A scratch test was applied to analyze cell migration, and a Transwell assay was used to test cell invasion ability. Western blotting was to measure matrix metalloproteinase (MMP)-2, MMP-9, and the tissue inhibitor of metalloproteinase 2 (TIMP2) protein levels. qRT-PCR indicated that LINC00312 expression was lower but miR-197-3p expression was higher in BC tissues compared with adjacent tissues; LINC00312 was negatively correlated with miR-197-3p. The migration test revealed that the downregulation of miR-197-3p and overexpression of LINC00312 inhibited cell migration and invasion abilities, while the overexpression of miR-197-3p and the upregulation of LINC00312 promoted cell migration and invasion. BC cells with downregulated miR-197-3p or upregulated LINC00312 had low MMP-2 and MMP-9 levels but high TIMP2. LINC00312 inhibited BC cell invasion and metastasis through mediating miR-197-3p.
authors.affiliation: Department of Urology, Huai'an First People's Hospital affiliated with Nanjing Medical University, No. 6 Beijing West Road, 223300, Huai'an, Jiangsu Province, People's Republic of China.
authors.forename: Yun-Yan
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Department of Urology, Huai'an Hospital affiliated of Xuzhou Medical College, Huai'an, 223002, People's Republic of China.
authors.forename: Zi-Yu
authors.initials: ZY
authors.lastname: Wu
authors.affiliation: Department of Urology, Huai'an First People's Hospital affiliated with Nanjing Medical University, No. 6 Beijing West Road, 223300, Huai'an, Jiangsu Province, People's Republic of China.
authors.forename: Gong-Cheng
authors.initials: GC
authors.lastname: Wang
authors.affiliation: Department of Urology, Huai'an First People's Hospital affiliated with Nanjing Medical University, No. 6 Beijing West Road, 223300, Huai'an, Jiangsu Province, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Urology, Huai'an First People's Hospital affiliated with Nanjing Medical University, No. 6 Beijing West Road, 223300, Huai'an, Jiangsu Province, People's Republic of China.
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Niu
authors.affiliation: Department of Urology, Huai'an First People's Hospital affiliated with Nanjing Medical University, No. 6 Beijing West Road, 223300, Huai'an, Jiangsu Province, People's Republic of China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Gu
authors.affiliation: Department of Urology, Huai'an First People's Hospital affiliated with Nanjing Medical University, No. 6 Beijing West Road, 223300, Huai'an, Jiangsu Province, People's Republic of China. Meng_31junsong@163.com.
authors.forename: Jun-Song
authors.initials: JS
authors.lastname: Meng
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: C470228:LINC00312 long non-coding RNA, human; C546085:MIRN197 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; C560823:TIMP2 protein, human; D019716:Tissue Inhibitor of Metalloproteinase-2; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Bladder cancer; Cell invasion; Cell migration; LINC00312; Long non-coding RNA; miR-197-3p