title: Fabrication of transparent quaternized PVA/silver nanocomposite hydrogel and its evaluation as an antimicrobial patch for wound care systems.
abstract: Grafting of quaternary nitrogen atoms into the backbone of polymer is an efficient way of developing new generation antimicrobial polymeric wound dressing. In this study, an elastic, non-adhesive and antimicrobial transparent hydrogel based dressing has been designed, which might be helpful for routine observation of wound area without removing the dressing material along with maintaining a sterile environment for a longer period of time. Green synthesized silver nanoparticles have been loaded into the quaternized PVA hydrogel matrix to improve its antimicrobial property. Silver nanoparticles loaded quaternized PVA hydrogel showed enhanced mechanical and swelling properties compared to native quaternized PVA hydrogel. Release kinetics evaluated by atomic absorption spectroscopy revealed that the release mechanism of silver nanoparticles from the hydrogel follows Fickian diffusion. Antimicrobial efficacy of the hydrogels was evaluated by disk diffusion test on Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. After 96 h of release in phosphate buffer, the growth inhibition zone created by silver nanoparticless loaded quaternized PVA hydrogel is comparable to that created by ampicillin. These observations assert that the silver nanoparticles loaded quaternized PVA hydrogel acts as a reservoir of silver nanoparticles, which helps in maintaining a sterile environment for longer time duration by releasing Ag nanocrystallite in sustained manner.
authors.affiliation: Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, Dresden, 01069, Germany.|Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.|Biomedical Engineering Unit, All India Institute of Medical Sciences, New Delhi, 110029, India.
authors.forename: Sirsendu
authors.initials: S
authors.lastname: Bhowmick
authors.affiliation: Stem Cell Facility, All India Institute of Medical Sciences, New Delhi, 110029, India.
authors.forename: Sujata
authors.initials: S
authors.lastname: Mohanty
authors.affiliation: Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India. veenak@cbme.iitd.ac.in.|Biomedical Engineering Unit, All India Institute of Medical Sciences, New Delhi, 110029, India. veenak@cbme.iitd.ac.in.
authors.forename: Veena
authors.initials: V
authors.lastname: Koul
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry*; D001458:Bandages; D004058:Diffusion; D004926:Escherichia coli / Q000187:drug effects; D055772:Green Chemistry Technology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000737:chemistry*; D013054:Spectrophotometry, Atomic; D013211:Staphylococcus aureus / Q000187:drug effects; D013314:Stress, Mechanical; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D020100:Hydrogels; D011108:Polymers; D012834:Silver