title: Generation of glucagon-like peptide-2-expressing Saccharomyces cerevisiae and its improvement of the intestinal health of weaned rats.
abstract: We aimed to assess the feasibility of enhancing the intestinal development of weaned rats using glucagon-like peptide-2 (GLP-2)-expressing Saccharomyces cerevisiae (S. cerevisiae). GLP-2-expressing S. cerevisiae (GLP2-SC) was generated using a recombinant approach. The diet of weaned rats was supplemented with the GLP2-SC strain. The average daily gain (ADG), the intestinal morphology and the activities of the digestive enzymes in the jejunum were tested to assess the influence of the GLP2-SC strain on intestinal development. The proliferation of rat enterocytes was also assessed in vitro. The study revealed that the ADG of the weaned rats that received GLP2-SC was significantly greater than that of the controls fed a basal diet (Control) and S. cerevisiae harbouring an empty vector (EV-SC) (P < 0.05) but was equivalent to that of positive control rats fed recombinant human GLP-2 (rh-GLP2) (P > 0.05). Furthermore, GLP2-SC significantly increased villous height (P < 0.01) and digestive enzyme activity (P < 0.05) in the jejunum. Immunohistochemistry analysis further affirmed that enterocyte proliferation was stimulated in rats fed the GLP2-SC strain, as indicated by the greater number of enterocytes stained with proliferative cell nuclear antigen (P < 0.05). In vitro, the proliferation of rat enterocytes was also stimulated by GLP-2 expressed by the GLP2-SC strain (P < 0.01). Herein, the combination of the GLP-2 approach and probiotic delivery constitute a possible dietary supplement for animals after weaning.
authors.affiliation: Department of Intensive Care Unit, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region, Chengdu, Sichuan, 610041, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Medical School, Chengdu University, Chengdu, Sichuan, 610041, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Cao
authors.affiliation: Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, Sichuan, 610041, China.
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Department of Intensive Care Unit, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China. zhouyanhxicu@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000821:Animal Feed; D000818:Animals; D001835:Body Weight; D049109:Cell Proliferation; D002478:Cells, Cultured; D004032:Diet / Q000379:methods*; D020895:Enterocytes / Q000502:physiology; D041981:Gastrointestinal Tract / Q000033:anatomy & histology / Q000201:enzymology / Q000254:growth & development* / Q000382:microbiology*; D053767:Glucagon-Like Peptide 2 / Q000235:genetics / Q000378:metabolism*; D006262:Health; D006867:Hydrolases / Q000032:analysis; D019936:Probiotics / Q000008:administration & dosage*; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D016896:Treatment Outcome
chemical_list: D053767:Glucagon-Like Peptide 2; D011994:Recombinant Proteins; D006867:Hydrolases