title: Light-Activated Triazabutadienes for the Modification of a Viral Surface.
abstract: Chemical crosslinking is a versatile tool for the examination of biochemical interactions, in particular host-pathogen interactions. We report the critical first step toward the goal of probing these interactions by the synthesis and use of a new heterobifunctional crosslinker containing a triazabutadiene scaffold. The triazabutadiene is stable to protein conjugation and liberates a reactive aryl diazonium species upon irradiation with 350 nm light. We highlight the use of this technology by modifying the surface of several proteins, including the dengue virus envelope protein.
authors.affiliation: Department of Chemistry and Biochemistry, University of Arizona, Building 41, Room 104, 1306 E University Boulevard, Tucson, AZ, 85721, USA.
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Jensen
authors.affiliation: Department of Chemistry and Biochemistry, University of Arizona, Building 41, Room 104, 1306 E University Boulevard, Tucson, AZ, 85721, USA.
authors.forename: Flora W
authors.initials: FW
authors.lastname: Kimani
authors.affiliation: Department of Chemistry and Biochemistry, University of Arizona, Building 41, Room 104, 1306 E University Boulevard, Tucson, AZ, 85721, USA.
authors.forename: John C
authors.identifier: 0000-0001-5822-4080
authors.initials: JC
authors.lastname: Jewett
mesh_terms: D002070:Butadienes / Q000737:chemistry* / Q000528:radiation effects*; D003432:Cross-Linking Reagents / Q000737:chemistry; D003716:Dengue Virus / Q000737:chemistry*; D015394:Molecular Structure; D013499:Surface Properties; D014466:Ultraviolet Rays; D014759:Viral Envelope Proteins / Q000737:chemistry*
chemical_list: D002070:Butadienes; D003432:Cross-Linking Reagents; D014759:Viral Envelope Proteins
keywords: aryl diazonium ion; bioconjugation; crosslinking; photochemistry; viruses