title: Functionalized Fullerene Increases NF-κB Activity and Blocks Genotoxic Effect of Oxidative Stress in Serum-Starving Human Embryo Lung Diploid Fibroblasts.
abstract: The influence of a water-soluble [60] fullerene derivative containing five residues of 3-phenylpropionic acid and a chlorine addend appended to the carbon cage (F-828) on serum-starving human embryo lung diploid fibroblasts (HELFs) was studied. Serum deprivation evokes oxidative stress in HELFs. Cultivation of serum-starving HELFs in the presence of 0.1-1 µM F-828 significantly decreases the level of free radicals, inhibits autophagy, and represses expression of NOX4 and NRF2 proteins. The activity of NF-κB substantially grows up in contrast to the suppressed NRF2 activity. In the presence of 0.2-0.25 µM F-828, the DSB rate and apoptosis level dramatically decrease. The maximum increase of proliferative activity of the HELFs and maximum activity of NF-κB are observed at these concentration values. Conclusion. Under the conditions of oxidative stress evoked by serum deprivation the water-soluble fullerene derivative F-828 used in concentrations of 0.1 to 1 µM strongly stimulates the NF-κB activity and represses the NRF2 activity in HELFs.
authors.affiliation: Research Centre for Medical Genetics (RCMG), Moscow 115478, Russia; V. A. Negovsky Research Institute of General Reanimatology, Moscow 107031, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Ershova
authors.affiliation: Research Centre for Medical Genetics (RCMG), Moscow 115478, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Sergeeva
authors.affiliation: Research Centre for Medical Genetics (RCMG), Moscow 115478, Russia.
authors.forename: V J
authors.initials: VJ
authors.lastname: Tabakov
authors.affiliation: Research Centre for Medical Genetics (RCMG), Moscow 115478, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Kameneva
authors.affiliation: Research Centre for Medical Genetics (RCMG), Moscow 115478, Russia.
authors.forename: L N
authors.identifier: 0000-0002-3664-6685
authors.initials: LN
authors.lastname: Porokhovnik
authors.affiliation: Institute for Problems of Chemical Physics of Russian Academy of Sciences, Moscow Region 142432, Russia.
authors.forename: I I
authors.initials: II
authors.lastname: Voronov
authors.affiliation: Institute for Problems of Chemical Physics of Russian Academy of Sciences, Moscow Region 142432, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Khakina
authors.affiliation: Institute for Problems of Chemical Physics of Russian Academy of Sciences, Moscow Region 142432, Russia; Skolkovo Institute of Science and Technology, Skolkovo Innovation Center, Nobel Street 3, Moscow 143026, Russia.
authors.forename: P A
authors.initials: PA
authors.lastname: Troshin
authors.affiliation: Research Centre for Medical Genetics (RCMG), Moscow 115478, Russia; N. I. Pirogov Russian National Research Medical University, Moscow 117997, Russia.
authors.forename: S I
authors.initials: SI
authors.lastname: Kutsev
authors.affiliation: Research Centre for Medical Genetics (RCMG), Moscow 115478, Russia; V. A. Negovsky Research Institute of General Reanimatology, Moscow 107031, Russia.
authors.forename: N N
authors.initials: NN
authors.lastname: Veiko
authors.affiliation: Research Centre for Medical Genetics (RCMG), Moscow 115478, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Kostyuk
mesh_terms: D001343:Autophagy / Q000187:drug effects; D059447:Cell Cycle Checkpoints; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004171:Diploidy; D004705:Endocytosis / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology; D005609:Free Radicals / Q000378:metabolism; D037741:Fullerenes / Q000494:pharmacology*; D006801:Humans; D008168:Lung / Q000166:cytology; D008856:Microscopy, Fluorescence; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D016895:Culture Media, Serum-Free; D005609:Free Radicals; D037741:Fullerenes; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495635:NFE2L2 protein, human; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases; C498910:NOX4 protein, human