title: Quantification of Carbohydrates and Related Materials Using Sodium Ion Adducts Produced by Matrix-Assisted Laser Desorption Ionization.
abstract: The utility of sodium ion adducts produced by matrix-assisted laser desorption ionization for the quantification of analytes with multiple oxygen atoms was evaluated. Uses of homogeneous solid samples and temperature control allowed the acquisition of reproducible spectra. The method resulted in a direct proportionality between the ion abundance ratio I([A + Na]+)/I([M + Na]+) and the analyte concentration, which could be used as a calibration curve. This was demonstrated for carbohydrates, glycans, and polyether diols with dynamic range exceeding three orders of magnitude. Graphical Abstract ᅟ.
authors.affiliation: Department of Chemistry, Seoul National University, Seoul, 151-747, Korea.
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Ahn
authors.affiliation: Department of Chemistry, Seoul National University, Seoul, 151-747, Korea.
authors.forename: Kyung Man
authors.initials: KM
authors.lastname: Park
authors.affiliation: Disease Target Structure Research Center, KRIBB, Daejeon, 305-806, Korea.
authors.forename: Jeong Hee
authors.initials: JH
authors.lastname: Moon
authors.affiliation: Department of Chemistry, Seoul National University, Seoul, 151-747, Korea.
authors.forename: Seong Hoon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Seoul National University Research Institute for Basic Sciences, Seoul, 151-747, Korea. myungsoo@snu.ac.kr.|Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul, 151-742, Korea. myungsoo@snu.ac.kr.
authors.forename: Myung Soo
authors.initials: MS
authors.lastname: Kim
keywords: Quantification by MALDI; Quantification of carbohydrate; Quantification of glycan; Sodium ion adduct