title: Novel Function of Sprouty4 as a Regulator of Stemness and Differentiation of Embryonic Stem Cells.
abstract: Sprouty (Spry) genes encode inhibitors of the receptor tyrosine kinase signaling cascade, which plays important roles in stem cells. However, the role of Spry4 in the stemness of embryonic stem cells has not been fully elucidated. Here, we used mouse embryonic stem cells (mESCs) as a model system to investigate the role of Spry4 in the stem cells. Suppression of Spry4 expression results in the decreases of cell proliferation, EB formation and stemness marker expression, suggesting that Spry4 activity is associated with stemness of mESCs. Teratoma assay showed that the cartilage maturation was facilitated in Spry4 knocked down mESCs. Our results suggest that Spry4 is an important regulator of the stemness and differentiation of mESCs.
authors.affiliation: Dept. of Biomedical Science, College of Life Science, CHA University, Seoul 06135, Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Dept. of Biomedical Science, College of Life Science, CHA University, Seoul 06135, Korea.
authors.forename: Sunghyun
authors.initials: S
authors.lastname: Park
authors.affiliation: Dept. of Biomedical Science, College of Life Science, CHA University, Seoul 06135, Korea.
authors.forename: Kwang-Soo
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Dept. of Biomedical Science, College of Life Science, CHA University, Seoul 06135, Korea.
authors.forename: Jeong-Jae
authors.initials: JJ
authors.lastname: Ko
authors.affiliation: Dept. of Biomedical Science, College of Life Science, CHA University, Seoul 06135, Korea.
authors.forename: Soohong
authors.initials: S
authors.lastname: Lee
authors.affiliation: Dept. of Life Science, Chung-Ang University, Seoul 06975, Korea.
authors.forename: Keun Pil
authors.initials: KP
authors.lastname: Kim
authors.affiliation: Dept. of Biomedical Science, College of Life Science, CHA University, Seoul 06135, Korea.
authors.forename: Kyung-Soon
authors.initials: KS
authors.lastname: Park
keywords: Differentiation; Embryonic stem cell; Sprouty4