title: Blood level of CD45+ platelets and development of restenosis after drug-eluting stent implantation in patients with stable coronary artery disease.
abstract: OBJECTIVE
  The aim of this study was to assess CD45-positive platelets (CD45+ platelets) involvement in restenosis development after drug-eluting stent (DES) implantation in patients with stable coronary artery disease (CAD).
  
  
  METHODS
  The study comprised 126 male and female patients with stable angina pectoris, who underwent elective coronary stenting with DES and follow-up angiography within 6-12 months. The patients were assigned to the group with restenosis (n = 53) or group without restenosis (n = 73) according to the follow-up angiograms. In both groups we compared the level in blood of CD45+ platelets, the clinical, laboratory and angiographic variables, which may affect the development of restenosis. We have also constructed a logit regression model for prognosis of restenosis occurrence after DES implantation.
  
  
  RESULTS
  The blood count of CD45+ platelets was higher in patients with restenosis than in patients without: 0.82 % (0.58; 1.12) vs. 0.34 % (0.20; 0.68), p < 0.001, data are expressed as median (lower quartile; upper quartile). By binary comparisons of more than 35 different clinical, laboratory and angiographic variables we identified 8 significant risk factors for the development of stent restenosis after DES. In order to define the risk of the development of restenosis, we have built a logit regression model. The resulting logit regression equation included the level of CD45+ platelets, the neutrophil to lymphocyte ratio (NLR), small diameter arteries stenting and the number of simultaneously implanted stents in one patient. Receiver operating characteristic (ROC) curve analysis has demonstrated the high prognostic value of the resulting logit regression equation with an area under the curve (AUC) of 0.91 % (p < 0.001).
  
  
  CONCLUSIONS
  The acquired data indicate the presence of a close relationship between circulating CD45+ platelets and restenosis development after DES implantation in patients with stable CAD.
authors.affiliation: Laboratory of Stem Cells, Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya Street 15A, 121552, Moscow, Russia. zufargabbasov@yandex.ru.
authors.forename: Zufar
authors.initials: Z
authors.lastname: Gabbasov
authors.affiliation: Laboratory of Stem Cells, Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya Street 15A, 121552, Moscow, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Kozlov
authors.affiliation: Laboratory of Stem Cells, Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya Street 15A, 121552, Moscow, Russia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Byazrova
authors.affiliation: Laboratory of Stem Cells, Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya Street 15A, 121552, Moscow, Russia.
authors.forename: Olga
authors.initials: O
authors.lastname: Saburova
authors.affiliation: Laboratory of Stem Cells, Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya Street 15A, 121552, Moscow, Russia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Melnikov
authors.affiliation: Comenius University and University Hospital, Mickiewiczova 13, 81369, Bratislava, Slovakia.
authors.forename: Martin
authors.initials: M
authors.lastname: Caprnda
authors.affiliation: Department of Cardiology, East Slovak Institute of Cardiovascular Diseases, Kosice, Slovakia.
authors.forename: Eduard
authors.initials: E
authors.lastname: Curilla
authors.affiliation: Comenius University and University Hospital, Mickiewiczova 13, 81369, Bratislava, Slovakia. ludovitgaspar@gmail.com.
authors.forename: Ludovit
authors.initials: L
authors.lastname: Gaspar
authors.affiliation: Department of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1946/1, 61242, Brno, Czech Republic. kruzliakpeter@gmail.com.|Masaryk University, Brno, Czech Republic. kruzliakpeter@gmail.com.
authors.forename: Peter
authors.initials: P
authors.lastname: Kruzliak
authors.affiliation: Laboratory of Stem Cells, Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya Street 15A, 121552, Moscow, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Smirnov
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000473:pathology*; D015984:Causality; D015897:Comorbidity; D003324:Coronary Artery Disease / Q000097:blood* / Q000453:epidemiology / Q000628:therapy*; D023903:Coronary Restenosis / Q000097:blood* / Q000453:epidemiology; D054855:Drug-Eluting Stents / Q000706:statistics & numerical data*; D005260:Female; D006083:Graft Occlusion, Vascular / Q000097:blood / Q000453:epidemiology; D006801:Humans; D017493:Leukocyte Common Antigens / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010976:Platelet Count / Q000706:statistics & numerical data; D015995:Prevalence; D016016:Proportional Hazards Models; D012189:Retrospective Studies; D018570:Risk Assessment / Q000379:methods; D012307:Risk Factors; D012426:Russia / Q000453:epidemiology
chemical_list: D015415:Biomarkers; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human
keywords: CD45+ platelets; Coronary artery disease; Drug-eluting stents; Inflammation; Restenosis