title: Aedes aegypti D7 Saliva Protein Inhibits Dengue Virus Infection.
abstract: Aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (DENV) types 1-4. Ae. aegypti transmits DENV by inoculating virus-infected saliva into host skin during probing and feeding. Ae. aegypti saliva contains over one hundred unique proteins and these proteins have diverse functions, including facilitating blood feeding. Previously, we showed that Ae. aegypti salivary gland extracts (SGEs) enhanced dissemination of DENV to draining lymph nodes. In contrast, HPLC-fractionation revealed that some SGE components inhibited infection. Here, we show that D7 proteins are enriched in HPLC fractions that are inhibitory to DENV infection, and that recombinant D7 protein can inhibit DENV infection in vitro and in vivo. Further, binding assays indicate that D7 protein can directly interact with DENV virions and recombinant DENV envelope protein. These data reveal a novel role for D7 proteins, which inhibits arbovirus transmission to vertebrates through a direct interaction with virions.
authors.affiliation: Foundational Sciences, Central Michigan University College of Medicine, Mt. Pleasant, Michigan, United States of America.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Conway
authors.affiliation: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina, United States of America.
authors.forename: Berlin
authors.initials: B
authors.lastname: Londono-Renteria
authors.affiliation: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina, United States of America.
authors.forename: Andrea
authors.initials: A
authors.lastname: Troupin
authors.affiliation: Center for Vaccine Research and Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
authors.forename: Alan M
authors.initials: AM
authors.lastname: Watson
authors.affiliation: Center for Vaccine Research and Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
authors.forename: William B
authors.initials: WB
authors.lastname: Klimstra
authors.affiliation: Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, United States of America.|Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America.
authors.forename: Erol
authors.initials: E
authors.lastname: Fikrig
authors.affiliation: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina, United States of America.
authors.forename: Tonya M
authors.initials: TM
authors.lastname: Colpitts
mesh_terms: D000330:Aedes / Q000737:chemistry / Q000821:virology*; D000818:Animals; D003715:Dengue / Q000188:drug therapy*; D003716:Dengue Virus / Q000187:drug effects*; D005260:Female; D006801:Humans; D019476:Insect Proteins / Q000494:pharmacology*; D051379:Mice; D012463:Saliva / Q000821:virology; D012469:Salivary Glands / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000494:pharmacology*; D020298:U937 Cells
chemical_list: D019476:Insect Proteins; D012471:Salivary Proteins and Peptides