title: Effect of Roux-en-Y Gastric Bypass on the NLRP3 Inflammasome in Pancreatic Islets from Zucker Diabetic Fatty Rats.
abstract: Diabetes and obesity are associated with inflammasome-mediated low-grade, chronic inflammation that may induce pancreatic beta-cell dysfunction and apoptosis. We examined the effects of Roux-en-Y gastric bypass (RYGB) surgery on NOD-like receptor family, pyrin domain containing-3 (NLRP3) inflammasome-related genes from pancreatic islets of Zucker diabetic fatty rats.
  
  
  
  Islets were collected from Zucker diabetic fatty sham control and RYGB, 30 days after surgery. We assessed expression of genes that regulate glucose metabolism and the NLRP3 inflammasome (NLRP3, caspase-1, IL-1β, IL-18, apoptosis-associated speck-like protein), IL-6, and monocyte chemoattractant protein-1.
  
  
  
  Gene expression for NLRP3 (p < 0.02), IL-1β (p < 0.04), and IL-6 (p < 0.01) was reduced by RYGB and positively correlated with change in body weight. IL-1β positively correlated with glucose AUC response.
  
  
  
  Suppression of the NLRP3 inflammasome in pancreatic islets may contribute to improved glycemic control after RYGB.
authors.affiliation: Biochemistry Department, Faculty of Biology, University of Bucharest, Bucharest, Romania.|Metabolic Translational Research Center, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: Andreea Oana
authors.initials: AO
authors.lastname: Mocanu
authors.affiliation: Department of Pathobiology, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: Anny
authors.initials: A
authors.lastname: Mulya
authors.affiliation: Department of Pathobiology, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: Hazel
authors.initials: H
authors.lastname: Huang
authors.affiliation: Bariatric Metabolic Institute, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: Olivia
authors.initials: O
authors.lastname: Dan
authors.affiliation: Bariatric Metabolic Institute, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: Philip R
authors.initials: PR
authors.lastname: Schauer
authors.affiliation: Biochemistry Department, Faculty of Biology, University of Bucharest, Bucharest, Romania.
authors.forename: Anca
authors.initials: A
authors.lastname: Dinischiotu
authors.affiliation: Bariatric Metabolic Institute, Cleveland Clinic, Cleveland, OH, USA.
authors.forename: Stacy A
authors.initials: SA
authors.lastname: Brethauer
authors.affiliation: Metabolic Translational Research Center, Cleveland Clinic, Cleveland, OH, USA. kirwanj@ccf.org.|Department of Pathobiology, Cleveland Clinic, Cleveland, OH, USA. kirwanj@ccf.org.|Department of Gastroenterology & Hepatology, Cleveland Clinic, Cleveland, OH, USA. kirwanj@ccf.org.
authors.forename: John P
authors.initials: JP
authors.lastname: Kirwan
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics; D003921:Diabetes Mellitus, Experimental; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000601:surgery*; D015536:Down-Regulation; D015390:Gastric Bypass; D015870:Gene Expression; D005786:Gene Expression Regulation; D005947:Glucose / Q000235:genetics; D058847:Inflammasomes / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000235:genetics*; D009765:Obesity / Q000235:genetics* / Q000503:physiopathology / Q000601:surgery; D051381:Rats; D011924:Rats, Zucker
chemical_list: D018932:Chemokine CCL2; D058847:Inflammasomes; D015850:Interleukin-6; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C573346:Nlrp3 protein, rat; D005947:Glucose
keywords: Beta-cells; Cellular regulation; Cytokine; Diabetes; Inflammation; Insulin resistance