title: Simultaneous determination of telmisartan and pitavastatin in rat plasma by UPLC-MS/MS: Application to pharmacokinetic interaction study.
abstract: To investigate the pharmacokinetic (PK) interaction between telmisartan (Tel) and pitavastatin (Pit), a rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometric assay method had been successfully established and fully validated for the simultaneous quantification of Tel and Pit in rat plasma. A simple protein precipitation procedure was adopted for the sample preparation with satisfactory extraction recovery for both analytes and the internal standard. The samples were chromatographed on an Inertsil ODS-3 C18 column (100mm×2.1mm, 2μm) using a mixture of acetonitrile and 10mM ammonium acetate containing 0.1% formic acid (60: 40, v/v) as the mobile phase at a flow rate of 0.4mL/min. The calibration curves obtained were linear (r>0.99) over the concentration range of 2-200ng/mL for Tel and 1-100ng/mL for Pit, respectively. The validated method was successfully applied to the PK study and the data did not reveal any evidence for the potential drug-drug interaction (DDI) between Tel and Pit. This information would provide the evidence for clinical rational use of Tel and Pit, and this study might be applied in therapeutic drug monitoring in patients receiving Tel/Pit combinations or single drug.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Xu
authors.affiliation: School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu 610041, Sichuan, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, China.
authors.forename: Dailong
authors.initials: D
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, China.
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Jian
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, China.
authors.forename: Minghai
authors.initials: M
authors.lastname: Tang
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu 610041, Sichuan, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu, China. Electronic address: songxrpaper@126.com.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Song
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000097:blood* / Q000493:pharmacokinetics*; D001565:Benzoates / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D008297:Male; D011804:Quinolines / Q000097:blood* / Q000493:pharmacokinetics*; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000077333:Telmisartan
chemical_list: D001562:Benzimidazoles; D001565:Benzoates; D011804:Quinolines; C108475:pitavastatin; D000077333:Telmisartan
keywords: Drug interaction; LC–MS/MS; Pharmacokinetics; Pitavastatin; Telmisartan