title: Chiral recognition of penicillamine enantiomers using hemoglobin and gold nanoparticles functionalized graphite-like carbon nitride nanosheets via electrochemiluminescence.
abstract: A new stable and stereo-selective electrochemiluminescence (ECL) interface has been designed for specific recognition of penicillamine (Pen) enantiomers by using hemoglobin (Hb) and gold nanoparticles functionalized graphite-like carbon nitride nanosheets composite (Au-g-C3N4NHs) modified glassy carbon electrodes (Hb/Au-g-C3N4/GCE). The advantages of Hb as chiral selector and Au-g-C3N4NHs as luminophore were perfectly displayed in this novel interface. The obviously different ECL intensity was exhibited after l-Pen and d-Pen adsorbed on Hb/Au-g-C3N4/GCE, and a larger response was observed on d-Pen/Hb/Au-g-C3N4/GCE. Under the optimum conditions, the developed ECL chiral sensor showed excellent analytical property for detection of Pen enantiomers in a linear range of 1.0×10-4M to 5.0×10-3M, and the detection limits of l-Pen and d-Pen were 3.1×10-5M and 3.3×10-5M (S/N=3) respectively. This work with high selectivity, stability and reproducibility may open a new door based on ECL to discriminate Pen enantiomers.
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Lin
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Qinghong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Xia
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Peiyao
authors.initials: P
authors.lastname: Ran
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: fyzc@swu.edu.cn.
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Fu
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009570:Nitriles / Q000737:chemistry*; D010396:Penicillamine / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D013053:Spectrophotometry; D013237:Stereoisomerism; D013499:Surface Properties
chemical_list: D006454:Hemoglobins; D009570:Nitriles; C011206:cyanogen; D006046:Gold; D006108:Graphite; D010396:Penicillamine
keywords: Chiral recognition; Electrochemiluminescence; Graphite-like carbon nitride; Hemoglobin; Penicillamine enantiomers