title: Combined Dielectrophoresis and Impedance Systems for Bacteria Analysis in Microfluidic On-Chip Platforms.
abstract: Bacteria concentration and detection is time-consuming in regular microbiology procedures aimed to facilitate the detection and analysis of these cells at very low concentrations. Traditional methods are effective but often require several days to complete. This scenario results in low bioanalytical and diagnostic methodologies with associated increased costs and complexity. In recent years, the exploitation of the intrinsic electrical properties of cells has emerged as an appealing alternative approach for concentrating and detecting bacteria. The combination of dielectrophoresis (DEP) and impedance analysis (IA) in microfluidic on-chip platforms could be key to develop rapid, accurate, portable, simple-to-use and cost-effective microfluidic devices with a promising impact in medicine, public health, agricultural, food control and environmental areas. The present document reviews recent DEP and IA combined approaches and the latest relevant improvements focusing on bacteria concentration and detection, including selectivity, sensitivity, detection time, and conductivity variation enhancements. Furthermore, this review analyses future trends and challenges which need to be addressed in order to successfully commercialize these platforms resulting in an adequate social return of public-funded investments.
authors.affiliation: Department of Electronics, Bioelectronics and Nanobioengineering Research Group (SIC-BIO), University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain. cpaezaviles@el.ub.edu.
authors.forename: Cristina
authors.initials: C
authors.lastname: Páez-Avilés
authors.affiliation: Department of Electronics, Bioelectronics and Nanobioengineering Research Group (SIC-BIO), University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain. ejuanola@el.ub.edu.
authors.forename: Esteve
authors.initials: E
authors.lastname: Juanola-Feliu
authors.affiliation: Department of Electronics, Bioelectronics and Nanobioengineering Research Group (SIC-BIO), University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain. jpunter@el.ub.edu.
authors.forename: Jaime
authors.initials: J
authors.lastname: Punter-Villagrasa
authors.affiliation: Department of Electronics, Bioelectronics and Nanobioengineering Research Group (SIC-BIO), University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain. bdelmoral@el.ub.edu.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Del Moral Zamora
authors.affiliation: Department of Electronics, Bioelectronics and Nanobioengineering Research Group (SIC-BIO), University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain. antoni.homs@icn2.cat.|IBEC-Institute of Bioengineering of Catalonia, Nanobioengineering Research Group, Baldiri Reixac 10-12, 08028 Barcelona, Spain. antoni.homs@icn2.cat.|CIBER-BBN-Biomedical Research Networking Centre for Bioengineering, Biomaterials and Nanomedicine, María de Luna 11, Edificio CEEI, 50018 Zaragoza, Spain. antoni.homs@icn2.cat.
authors.forename: Antoni
authors.initials: A
authors.lastname: Homs-Corbera
authors.affiliation: Department of Electronics, Bioelectronics and Nanobioengineering Research Group (SIC-BIO), University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain. jcolomer@el.ub.edu.
authors.forename: Jordi
authors.initials: J
authors.lastname: Colomer-Farrarons
authors.affiliation: Department of Electronics, Bioelectronics and Nanobioengineering Research Group (SIC-BIO), University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain. pmiribel@el.ub.edu.
authors.forename: Pere Lluís
authors.initials: PL
authors.lastname: Miribel-Català
authors.affiliation: Department of Electronics, Bioelectronics and Nanobioengineering Research Group (SIC-BIO), University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain. jsamitier@ibecbarcelona.eu.|IBEC-Institute of Bioengineering of Catalonia, Nanobioengineering Research Group, Baldiri Reixac 10-12, 08028 Barcelona, Spain. jsamitier@ibecbarcelona.eu.|CIBER-BBN-Biomedical Research Networking Centre for Bioengineering, Biomaterials and Nanomedicine, María de Luna 11, Edificio CEEI, 50018 Zaragoza, Spain. jsamitier@ibecbarcelona.eu.
authors.forename: Josep
authors.initials: J
authors.lastname: Samitier
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D002021:Buffers; D017097:Electric Impedance; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D018581:Technology Transfer
chemical_list: D002021:Buffers
keywords: bacteria; dielectrophoresis; impedance; microfluidics; on-chip