title: Development of putative probiotics as feed additives: validation in a porcine-specific gastrointestinal tract model.
abstract: Enforced restrictions on the use of antibiotics as growth promoters (AGPs) in animal production have prompted investigations into alternative feed additives in recent decades. Probiotics are currently the main feed additive used in livestock. However, the selection of probiotic candidates relies on human-based methods and little is known about the verification criteria for host-specific selection. We investigated the probiotic potential of Lactobacillus salivarius strains isolated from fed pig feces for their use as porcine feed additives. Two methods were developed that simulated the pig gastrointestinal (GI) tract and the intestinal epithelium, and these were compared with human-based in vitro methods and used for selecting porcine probiotics. Lactobacillus salivarius strain LS6 was identified as a promising probiotic strain for potential use as a porcine feed additive. This strain prevented disruption of the epithelial integrity of pig small intestine (PSI) cells by inhibiting the adherence of enterotoxigenic Escherichia coli K88. It also showed high survival rates in the in vitro pig GI tract model and good adhesion to PSI cells. We propose that host target-specific screening and validation methods are important tools in the development of effective probiotic feed additives, and this approach may support future-oriented agriculture.
authors.affiliation: Research Institute of Eco-friendly Livestock Science, Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang, Gangwon-do, 232-916, South Korea.
authors.forename: Soyoung
authors.initials: S
authors.lastname: Yeo
authors.affiliation: Research Institute of Eco-friendly Livestock Science, Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang, Gangwon-do, 232-916, South Korea.
authors.forename: Suro
authors.initials: S
authors.lastname: Lee
authors.affiliation: Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang, Gyeongbuk, 791-708, South Korea.
authors.forename: Hyunjoon
authors.initials: H
authors.lastname: Park
authors.affiliation: School of Life Sciences, Handong Global University, Pohang, Gyeongbuk, 791-708, South Korea.
authors.forename: Heuynkil
authors.initials: H
authors.lastname: Shin
authors.affiliation: Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Pohang, Gyeongbuk, 791-708, South Korea.
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Holzapfel
authors.affiliation: Research Institute of Eco-friendly Livestock Science, Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang, Gangwon-do, 232-916, South Korea. chulsunghuh@gmail.com.|Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang, Gangwon-do, 232-916, South Korea. chulsunghuh@gmail.com.
authors.forename: Chul Sung
authors.initials: CS
authors.lastname: Huh
mesh_terms: D000821:Animal Feed; D000818:Animals; D000898:Antibiosis; D001422:Bacterial Adhesion; D019587:Dietary Supplements; D004847:Epithelial Cells / Q000382:microbiology; D004926:Escherichia coli / Q000502:physiology; D041981:Gastrointestinal Tract / Q000382:microbiology*; D000069996:Ligilactobacillus salivarius / Q000502:physiology*; D050296:Microbial Viability; D008954:Models, Biological; D019936:Probiotics / Q000008:administration & dosage*; D013552:Swine
keywords: Adhesion ability; Feed additives; Gastrointestinal tract; Lactobacillus salivarius; Porcine; Probiotics