title: Protective effects of ginsenoside Rg1 against hydrogen peroxide-induced injury in human neuroblastoma cells.
abstract: The active ingredient of ginseng, ginsenosides Rg1, has been shown to scavenge free radicals and improve antioxidant capacity. This study hypothesized that ginsenosides Rg1 has a protective role in human neuroblastoma cells injured by H2O2. Ginsenosides Rg1 at different concentrations (50 and 100 μM) was used to treat H2O2 (150 μM)-injured SH-SY5Y cells. Results demonstrated that ginsenoside Rg1 elevated the survival rate of SH-SY5Y cells injured by H2O2, diminished the amount of leaked lactate dehydrogenase, and increased superoxide dismutase activity. Ginsenoside Rg1 effectively suppressed caspase-3 immunoreactivity, and contributed to heat shock protein 70 gene expression, in a dose-dependent manner. These results indicate that ginsenoside Rg1 has protective effects on SH-SY5Y cells injured by H2O2 and that its mechanism of action is associated with anti-oxidation and the inhibition of apoptosis.
authors.affiliation: Department of Traditional Chinese Medicine, Hainan Branch Hospital of Chinese PLA General Hospital, Sanya, Hainan Province, China.
authors.forename: Zhi-Gao
authors.initials: ZG
authors.lastname: Sun
authors.affiliation: Department of Traditional Chinese Medicine, Chinese PLA General Hospital, Beijing, China.
authors.forename: Li-Ping
authors.identifier: 0000-0002-5568-9293
authors.initials: LP
authors.lastname: Chen
authors.affiliation: Department of Traditional Chinese Medicine, Chinese PLA General Hospital, Beijing, China.
authors.forename: Fa-Wei
authors.initials: FW
authors.lastname: Wang
authors.affiliation: Department of Traditional Chinese Medicine, Hainan Branch Hospital of Chinese PLA General Hospital, Sanya, Hainan Province, China.
authors.forename: Cheng-Yong
authors.initials: CY
authors.lastname: Xu
authors.affiliation: Institute of Gerontology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Geng
keywords: H2O2; SH-SY5Y cells; caspase-3; cell apoptosis; cerebral ischemia; dose-effect relationship; ginsenoside Rg1; heat shock protein 70; lactate dehydrogenase; nerve regeneration; neural regeneration; superoxide dismutase; traditional Chinese medicine monomer