title: Design and cytocompatibility of chitosan-based thermoresponsive cell culture plates.
abstract: BACKGROUND
  The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).
  
  
  METHODS
  Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)-induced graft polymerization. The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively. Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
  
  
  RESULTS
  The modified TCPS exhibited good hydrophobic/hydrophilic property alterations in response to temperature. The cytocompatibility of the materials was improved due to the introduction of CS. Cells could be spontaneously detached from the surface without any damage, by controlling environmental temperature. The viability of cells obtained by temperature induction was higher than that obtained by enzymatic digestion.
  
  
  CONCLUSIONS
  This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.
authors.affiliation: School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin - PR China.|Tianjin Key Laboratory of Fiber Modification and Functional Fiber, Tianjin Polytechnic University, Tianjin - PR China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: He
authors.affiliation: School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin - PR China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin - PR China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Jin
authors.affiliation: School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin - PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Su
authors.affiliation: School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin - PR China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: An
authors.affiliation: School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin - PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ge
authors.affiliation: College of Life Sciences, Nankai University, Tianjin - PR China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Wei
authors.affiliation: Tianjin Key Laboratory of Fiber Modification and Functional Fiber, Tianjin Polytechnic University, Tianjin - PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D005347:Fibroblasts / Q000145:classification / Q000378:metabolism*; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D011437:Propylamines / Q000737:chemistry*
chemical_list: D011437:Propylamines; D048271:Chitosan; C035263:2-propylamine