title: Rapid molecular diagnostic test for Zika virus with low demands on sample preparation and instrumentation.
abstract: Zika virus has only recently gained attention due to recent large outbreaks worldwide. An easy to use nucleic acid amplification test could play an important role in the early detection of the infection and patient management. Here, we report a rapid and robust isothermal nucleic acid amplification assay for the detection of Zika virus. The method is cost-effective and compatible with portable instrumentation, enabling near patient testing and field use.
authors.affiliation: Research and Development, Orion Diagnostica Oy, P. O. BOX 83, FI-02101 Espoo, Finland. Electronic address: kevin.eboigbodin@oriondiagnostica.fi.
authors.forename: Kevin E
authors.initials: KE
authors.lastname: Eboigbodin
authors.affiliation: Research and Development, Orion Diagnostica Oy, P. O. BOX 83, FI-02101 Espoo, Finland.
authors.forename: Mirko
authors.initials: M
authors.lastname: Brummer
authors.affiliation: Research and Development, Orion Diagnostica Oy, P. O. BOX 83, FI-02101 Espoo, Finland.
authors.forename: Tuomas
authors.initials: T
authors.lastname: Ojalehto
authors.affiliation: Molecular Biology, GeneForm Technologies, Broadstairs, United Kingdom.
authors.forename: Mark
authors.initials: M
authors.lastname: Hoser
mesh_terms: D003362:Cost-Benefit Analysis; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D013997:Time Factors; D000071244:Zika Virus / Q000235:genetics / Q000302:isolation & purification*; D000071243:Zika Virus Infection / Q000175:diagnosis*
keywords: Amplification; Diagnostics; Isothermal; Outbreak; Portable; RNA; Virus; Zika