title: Biodegradation of Phenanthrene by Pseudomonas sp. JPN2 and Structure-Based Degrading Mechanism Study.
abstract: The strain Pseudomonas sp. JPN2 had a high potential to degrade phenanthrene degrading 98.52 % of the initial amount of 100 mg L-1after 10 days incubation. The analysis of metabolites demonstrated that the cleavage of phenanthrene started at the C9and C10positions on the aromatic ring by the dioxygenation reaction, and then further degraded via a phthalate pathway. To understand the interaction between phenanthrene and the amino acid residues in the active site of the target enzyme, a molecular docking simulation was performed. The results showed that the distances of C9-O1and C10-O2atoms were 3.47 and 3.67 Å, respectively. The C9and C10positions of the phenanthrene ring are much closer to the dioxygen molecule in the active site relative to the other atoms. Therefore, the C9and C10positions are vulnerable to attack in the initial oxygenation process.
authors.affiliation: School of Civil and Environmental Engineering, University of Science and Technology Beijing, No. 30 Xueyuan Road, Haidian District, Beijing, 100083, China.
authors.forename: Jingnan
authors.initials: J
authors.lastname: Jin
authors.affiliation: School of Civil and Environmental Engineering, University of Science and Technology Beijing, No. 30 Xueyuan Road, Haidian District, Beijing, 100083, China. yaojun@ustb.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: College of Science, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Qingye
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D001673:Biodegradation, Environmental; D062105:Molecular Docking Simulation; D010616:Phenanthrenes / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*
chemical_list: D010616:Phenanthrenes; C031181:phenanthrene
keywords: Biodegradation; Molecular docking; Phenanthrene; Pseudomonas sp. JPN2