title: Whole cell based microcontact imprinted capacitive biosensor for the detection of Escherichia coli.
abstract: In this study, a label-free, selective and sensitive microcontact imprinted capacitive biosensor was developed for the detection of Escherichia coli. The recognition of E. coli was successfully performed by this sensor prepared with the combination of microcontact imprinting method and capacitive biosensor technology. After preparation of bacterial stamps, microcontact-E. coli imprinted gold electrodes were generated using an amino acid based recognition element, N-methacryloyl-L-histidine methylester (MAH), 2-Hydroxyethyl methacrylate (HEMA) as monomers and ethyleneglycol dimethacrylate (EGDMA) as crosslinker under UV-polymerization. Real-time E. coli detection experiments were carried out within the range of 1.0×102-1.0×107CFU/mL. The unique combination of these two techniques provides selective detection with a detection limit of 70CFU/mL. The designed capacitive sensor has high selectivity and was able to distinguish E. coli when present together with competing bacterial strains which are known to have similar shape. In addition, the prepared sensor has the ability to detect E. coli with a recovery of 81-97% in e.g. river water.
authors.affiliation: Department of Biotechnology, Lund University, Lund, Sweden; Department of Biology, Faculty of Sciences, Hacettepe University, Ankara, Turkey.
authors.forename: Neslihan
authors.initials: N
authors.lastname: Idil
authors.affiliation: Department of Biotechnology, Lund University, Lund, Sweden; CapSenze Biosystems AB, Lund, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Hedström
authors.affiliation: Department of Chemistry, Faculty of Sciences, Hacettepe University, Ankara, Turkey.
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
authors.affiliation: Department of Biotechnology, Lund University, Lund, Sweden; CapSenze Biosystems AB, Lund, Sweden. Electronic address: bo.mattiasson@biotek.lu.se.
authors.forename: Bo
authors.initials: B
authors.lastname: Mattiasson
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D025802:Electric Capacitance; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004867:Equipment Design; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology; D006639:Histidine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D054802:Molecular Imprinting / Q000295:instrumentation / Q000379:methods*; D045483:Rivers / Q000382:microbiology*
chemical_list: D003432:Cross-Linking Reagents; D008689:Methacrylates; C491950:N-methacryloyl-L-histidine methyl ester; D006639:Histidine; C005044:hydroxyethyl methacrylate
keywords: Capacitive biosensor; Escherichia coli; Microcontact imprinting; N-methacryloyl-L-histidine methylester