title: Synthesis of Hexagonal ZnO-PQ7 Nano Disks Conjugated with Folic Acid to Image MCF - 7 Cancer Cells.
abstract: Surface modified ZnO nanomaterial is widely used in the field of bioimaging worldwide due to its optical properties, electronic characteristics and biocompatibility. Fluorescent enhanced, Polyquaternium-7(PQ7) capped, ZnO hexagonal nano disks (ZnO-PQ7) were synthesised by simple wet chemical method. The structural and optical properties of ZnO-PQ7 hexagonal nano disks were characterized using XRD, UV-Visible, Fluorescence, HRTEM, EDAX and FTIR studies. The size of synthesised ZnO-PQ7 were around 30-45 nm as confirmed by HRTEM studies. Fluorescence emission intensity increased with increase in PQ7 concentration. ZnO-PQ7 was further conjugated with folic acid (FA) to target human breast cancer cell line (MCF-7) via EDC/NHS coupling chemistry. Conjugation of folic acid with ZnO-PQ7 was confirmed by FTIR studies. The cell viability study using Methyl thiazolyltetrazolium(MTT) assay has demonstrated that the ZnO-PQ7 conjugated FA composites (ZnO-PQ7-FA) exhibit low toxicity towards MCF-7 up to a concentration of 125 μg/mL. Confocal laser scanning microscopic images confirmed the uptake of ZnO-PQ7-FA nanoparticles by MCF-7 cells. This study reveals ZnO-PQ7-FA nano disks as a potential imaging agent for detection of cancer cells. The synthesis route reported in this article is simple and easy to follow for the synthesis of ZnO-PQ7-FA in bulk quantities with high purity.
authors.affiliation: Department of Chemistry, Rajalakshmi Engineering College, Thandalam, Chennai, 602 105, India.
authors.forename: S
authors.initials: S
authors.lastname: Sureshkumar
authors.affiliation: Department of Chemistry, Rajalakshmi Engineering College, Thandalam, Chennai, 602 105, India.
authors.forename: B
authors.initials: B
authors.lastname: Jothimani
authors.affiliation: Department of Analytical Chemistry, University of Madras, Maraimalai campus, Guindy, Chennai, 600 025, India.
authors.forename: T M
authors.initials: TM
authors.lastname: Sridhar
authors.affiliation: Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai, 602 105, India.
authors.forename: Arul
authors.initials: A
authors.lastname: Santhosh
authors.affiliation: SRM Easwari Engineering College, Ramapuram, Chennai, 600089, India. bv1461967@gmail.com.
authors.forename: B
authors.initials: B
authors.lastname: Venkatachalapathy
mesh_terms: D000178:Acrylamides; D000643:Ammonium Chloride; D003952:Diagnostic Imaging; D005492:Folic Acid; D006801:Humans; D061986:MCF-7 Cells; D053758:Nanoparticles
chemical_list: D000178:Acrylamides; D000643:Ammonium Chloride; C449580:polyquaternium-7; D005492:Folic Acid
keywords: Cytotoxicity; Fluorescence; MCF-7 cell imaging; ZnO- PQ7-folic acid