title: Endocan reduces the malign grade of gastric cancer cells by regulating associated protein expression.
abstract: Endocan, which has been identified to be low expressed in gastric cancer, was found to be positively related to the differentiation level of gastric cancer in vivo and in vitro. In the present study, we aimed to investigate the role of endocan in gastric adenocarcinoma cell line SGC7901 by artificially upregualting or downregulating endocan expression using endocan recombinant vector or specific small interfering RNA (siRNA)-targeting endocan gene, respectively. The effects of endocan recombinant vector-mediated over-expressing and siRNA-mediated endocan silencing on the differentiation, migration, and apoptosis of SGC7901 cells were assessed. Furthermore, the primary molecular mechanisms of endocan were explored by testing the expression alterations of associated protein in SGC7901 along endocan over-expression or knockdown. We found that over-expression of endocan reduced the migration but promoted the differentiation and apoptosis of SGC7901 cells. While, knockdown of endocan did just the opposite. Some molecules were found to participate in endocan-mediated anti-tumor effects, such as p53, caspase 3, and MMP-9. In conclusion, our findings suggest that endocan plays an anti-carcinogenic role in gastric cancer development and progression and might serve as a prognostic biomarker as well as a potential therapeutic target for gastric cancer.
authors.affiliation: Laboratory of Molecular Biology and Department of Biochemistry, Key Laboratory of Gene Research of Anhui Province, Hefei, Anhui, People's Republic of China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Sumei
authors.affiliation: Laboratory of Molecular Biology and Department of Biochemistry, Key Laboratory of Gene Research of Anhui Province, Hefei, Anhui, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Shaolong
authors.affiliation: Laboratory of Molecular Biology and Department of Biochemistry, Key Laboratory of Gene Research of Anhui Province, Hefei, Anhui, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiang
authors.affiliation: Laboratory of Molecular Biology and Department of Biochemistry, Key Laboratory of Gene Research of Anhui Province, Hefei, Anhui, People's Republic of China.|General Department of Hyperbaric Oxygen, The Second People's Hospital of Hefei, Hefei, Anhui, 230011, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yinliang
authors.affiliation: Laboratory of Molecular Biology and Department of Biochemistry, Key Laboratory of Gene Research of Anhui Province, Hefei, Anhui, People's Republic of China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Qing
authors.affiliation: Laboratory of Molecular Biology and Department of Biochemistry, Key Laboratory of Gene Research of Anhui Province, Hefei, Anhui, People's Republic of China. aydesm-1@163.com.
authors.forename: Wang
authors.initials: W
authors.lastname: Yuan
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D017209:Apoptosis / Q000235:genetics*; D053148:Caspase 3 / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics*; D011509:Proteoglycans / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: C101134:ESM1 protein, human; D009363:Neoplasm Proteins; D011509:Proteoglycans; D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53; D053148:Caspase 3; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Biomarker; Endocan; Gastric cancer