title: [Metabolome analysis of solid tumors].
abstract: Metabolomics, the newest of the omics sciences that also include genomics, transcriptomics and proteomics, has matured into a reliable high-throughput technology. Gas chromatography combined with time-of-flight mass spectrometry (GC-TOFMS) is a suitable method to analyze the central metabolism in fresh frozen tumor tissue samples. Bioinformatics methods, including the PROFILE clustering developed by us, permit integrated analysis and fast interpretation of metabolomics data in the context of enzymatic reactions and metabolic pathways. The metabolome analyses of three solid tumor types presented here, together with the results of other authors, show that metabolites are suitable as biomarkers and provide diverse options for translation into the clinical setting.
authors.affiliation: Institut für Pathologie, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117, Berlin, Deutschland. jan.budczies@charite.de.
authors.forename: J
authors.initials: J
authors.lastname: Budczies
mesh_terms: D000612:4-Aminobutyrate Transaminase / Q000378:metabolism; D014408:Biomarkers, Tumor; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D018698:Glutamic Acid / Q000378:metabolism; D005972:Glutaminase / Q000037:antagonists & inhibitors; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D055442:Metabolome / Q000187:drug effects / Q000502:physiology*; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology*; D009376:Neoplasms, Hormone-Dependent / Q000188:drug therapy / Q000473:pathology; D010051:Ovarian Neoplasms / Q000473:pathology; D010053:Ovary / Q000473:pathology; D015091:beta-Alanine / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D005973:Glutamine; D015091:beta-Alanine; D018698:Glutamic Acid; D000612:4-Aminobutyrate Transaminase; D005972:Glutaminase
keywords: Beta-alanine; Glutamic acid; Glutaminase inhibitors; Metabolomics; Neoplasms