title: IGFBP1 Involved in the Decreased Birth Weight Due to Fetal High Estrogen Exposure in Mice.
abstract: Our previous study indicated that maternal high-estrogen environment in the first trimester is correlated with increased risks of low birth weight (LBW) and adult diseases. The present study aimed to establish an animal model to confirm such an effect in mice, and to further explore the mechanism involved. A mouse model with high estradiol (E2) exposure during early pregnancy was established, and the birth weight, growth after birth, and expression levels of insulin-like growth factor-binding protein 1 (IGFBP1) of pups were examined. Meanwhile, IGFBP1 expression after treatment of E2was examined in a HepG2 hepatoma cell line. We found that after exposure to a high-E2environment the weight of the pups decreased significantly, not only before but also after birth. Meanwhile, both mRNA and protein expressions of IGFBP1 were elevated in placenta and liver tissues. Furthermore, the level of IGFBP1 in the HepG2 cell line was elevated by the treatment of E2, whereas this effect was blocked by estrogen receptor antagonist ICI 182780. In summary, maternal high estrogen up-regulates expression of IGFBP1 in placenta and fetal livers, which contributes to LBW and decreases body weight in offspring.
authors.affiliation: The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jin
authors.affiliation: Key Laboratory of Reproductive Genetics, Ministry of Education (Zhejiang University), Hangzhou, China.
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Lv
authors.affiliation: Key Laboratory of Reproductive Genetics, Ministry of Education (Zhejiang University), Hangzhou, China.
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Yu
authors.affiliation: The First Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou, China.
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Shen
authors.affiliation: The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China doctorfc@zju.edu.cn.|Key Laboratory of Reproductive Genetics, Ministry of Education (Zhejiang University), Hangzhou, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Feng
authors.affiliation: Key Laboratory of Reproductive Genetics, Ministry of Education (Zhejiang University), Hangzhou, China.|International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: He-Feng
authors.initials: HF
authors.lastname: Huang
mesh_terms: D000818:Animals; D001724:Birth Weight / Q000187:drug effects* / Q000502:physiology; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D000077267:Fulvestrant; D056945:Hep G2 Cells; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D051379:Mice; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy
chemical_list: D004965:Estrogen Antagonists; D004967:Estrogens; D018970:Insulin-Like Growth Factor Binding Protein 1; D000077267:Fulvestrant; D004958:Estradiol
keywords: high estrogen exposure; insulin-like growth factor-binding protein 1 (IGFBP1); low birth weight; mice