title: The Use of Mass Spectrometric Techniques to Differentiate Isobaric and Isomeric Flavonoid Conjugates from Axyris amaranthoides.
abstract: Flavonoids are a group of compounds that are commonly found in various plants, where they play important roles in many processes, including free radical scavenging and UV protection. These compounds can also act as chemical messengers, physiological regulators or protectants against pathogens in the defense reactions of plants. Flavonoid activity is regulated by the addition of various substituents, usually mono- or oligosaccharides of common sugars, such as glucose, rhamnose or galactose. In some plants, glucuronic acid is attached, and this sugar is often acylated by phenylpropanoic acids. Identification of these compounds and their derivatives is of great importance to understanding their role in plant metabolism and defense mechanisms; this research is important because flavonoids are frequently a significant constituent of the human diet. In this study, we identify the flavonoid conjugates present inAxyris amaranthoidesL. extracts and demonstrate the usefulness of high-resolution mass spectrometry (HRMS) analyzers for the differentiation of isobaric compounds and the utility of fragmentation spectra for the differentiation of isomeric structures. According to our knowledge, some of the structures, especially dehydrodiferulated conjugates of tricin, whose structures are proposed here have been found for the first time in plant material.
authors.affiliation: European Centre for Bioinformatics and Genomics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland. lukasmar@ibch.poznan.pl.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Marczak
authors.affiliation: Department of Pharmacognosy, Poznan University of Medical Sciences, Świecickiego 4, 60-781 Poznan, Poland. paulina.znajdek@gmail.com.
authors.forename: Paulina
authors.initials: P
authors.lastname: Znajdek-Awiżeń
authors.affiliation: Department of Pharmacognosy, Poznan University of Medical Sciences, Świecickiego 4, 60-781 Poznan, Poland. wieslawabylka@o2.pl.
authors.forename: Wiesława
authors.initials: W
authors.lastname: Bylka
keywords: Axyris amaranthoides; collision-induced dissociation; electrospray; flavonoid conjugates; fragmentation spectra; isobaric compounds; isomeric compounds; tandem mass spectrometry