title: Application of CRISPR/Cas9 system in breeding of new antiviral plant germplasm.
abstract: With the development and improvement of CRISPR/Cas9 system in genomic editing technology, the system has been applied to the prevention and control of animal viral infectious diseases, which has made considerable achievements. It has also been applied to the study of highly efficient gene targeting editing in plant virus genomes. The CRISPR/Cas9-mediated targeted gene modification has not only achieved the genome editing of plant DNA virus, but also showed the genome editing potential of plant RNA virus. In addition, the CRISPR/Cas9 system functions at the gene transcriptional and post-transcriptional level, indicating that the system could regulate the replication of plant viruses through different ways. Compared with other plant viral disease control strategies, this system is more accurate in genome editing, more stable in gene expression regulation, and has broader spectrum of resistance to virus disease. In this review, we summarized the advantages, main problems and development tendency of CRISPR/cas9 system in breeding of new antiviral plant germplasms.
authors.affiliation: Hunan Crop Research Institute, Changsha 410125, China.
authors.forename: Dao-wei
authors.initials: DW
authors.lastname: Zhang
authors.affiliation: 1. Hunan Crop Research Institute, Changsha 410125, China; 2. Longping Branch of Graduate School of Central South University, Changsha 410125, China;
authors.forename: Chao-fan
authors.initials: CF
authors.lastname: Zhang
authors.affiliation: Longping Branch of Graduate School of Central South University, Changsha 410125, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Dong
authors.affiliation: Hunan Crop Research Institute, Changsha 410125, China.
authors.forename: Yan-lan
authors.initials: YL
authors.lastname: Huang
authors.affiliation: Hunan Crop Research Institute, Changsha 410125, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Hunan Crop Research Institute, Changsha 410125, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D001947:Breeding / Q000379:methods; D064113:CRISPR-Cas Systems / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D000072669:Gene Editing / Q000379:methods; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D010942:Plant Viruses / Q000235:genetics; D010944:Plants / Q000235:genetics* / Q000821:virology*; D014777:Virus Diseases / Q000235:genetics* / Q000821:virology
chemical_list: D018744:DNA, Plant