title: The inner membrane protein YhiM is necessary for Escherichia coli growth at high temperatures and low osmolarity.
abstract: To survive, Escherichia coli must be able to survive in rapidly changing environmental conditions including changes in temperature and osmolarity. We have studied the role of the inner membrane protein YhiM in changing environmental conditions. Our data indicate that YhiM is required for normal growth at 37 and 41 °C but not 21 °C. YhiM-deficient cells grown at high temperatures spend more time in lag phase and stop growing at lower cell densities in comparison with their wild-type counterparts. They also have growth defects in low NaCl medium at 37 °C and do not grow at all at 41 °C. The effects of low NaCl can be rescued by addition of KCl or sucrose to the low salt medium. Finally, YhiM-deficient cells fail to grow in dilute medium at 41 °C. These data suggest that YhiM may be important in protecting the cells from changes in temperature and osmolarity.
authors.affiliation: Department of Biology, University of Southern Indiana, 8600 University Blvd, Evansville, IN, 47712, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Anderson
authors.affiliation: Department of Biology, University of Southern Indiana, 8600 University Blvd, Evansville, IN, 47712, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Mann
authors.affiliation: Department of Biology, University of Southern Indiana, 8600 University Blvd, Evansville, IN, 47712, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Evans
authors.affiliation: Department of Biology, University of Southern Indiana, 8600 University Blvd, Evansville, IN, 47712, USA. rlsparksth@usi.edu.
authors.forename: R L
authors.identifier: http://orcid.org/0000-0003-0202-9360
authors.initials: RL
authors.lastname: Sparks-Thissen
mesh_terms: D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006358:Hot Temperature; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009994:Osmolar Concentration; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C579518:YhiM protein, E coli; D012965:Sodium Chloride
keywords: Escherichia coli (E. coli); Growth conditions; Inner membrane protein; Osmotic stress