title: Tessaracoccus defluvii sp. nov., isolated from an aeration tank of a sewage treatment plant.
abstract: A Gram-positive, non-motile, aerobic, coccus-shaped bacterium, designated strain LNB-140T, was isolated from a sewage treatment plant in the Republic of Korea and was characterised using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain LNB-140Tbelongs to genus Tessaracoccus in the family Propionibacteriaceae of the phylum Actinobacteria. The 16S rRNA gene sequence similarities between strain LNB-140Tand type strains of the genus, Tessaracoccus flavescens SST-39Tand Tessaracoccus rhinocerotis YIM101269Tare 97.8 and 97.4 %, respectively. The chemotaxonomic properties of strain LNB-140Tare consistent with those of members of the genus Tessaracoccus: a quinone system with MK-9(H4) as the predominant menaquinone; anteiso-C15:0and iso C15:0as the predominant cellular fatty acids; and LL-2,6-diaminopimelic acid as the diagnostic peptidoglycan diamino acid. The major polar lipids were identified as diphosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was determined to be 67.1 mol%. Differential phenotypic properties along with low DNA-DNA relatedness (<30 ± 3.2 %) with closely related type strains show that strain LNB-140Tis distinct from previously described members of the genus Tessaracoccus and represents a novel species in this genus, for which the name Tessaracoccus defluvii sp. nov. is proposed. The type strain is LNB-140T(=KEMB 5401-076T= JCM 17540T).
authors.affiliation: Department of Life Science, Kyonggi University, Suwon-si, Gyeonggi-do, 442-760, Republic of Korea.
authors.forename: Sathiyaraj
authors.initials: S
authors.lastname: Srinivasan
authors.affiliation: Department of Life Science, Kyonggi University, Suwon-si, Gyeonggi-do, 442-760, Republic of Korea.
authors.forename: Aravind
authors.initials: A
authors.lastname: Sundararaman
authors.affiliation: Department of Life Science, Kyonggi University, Suwon-si, Gyeonggi-do, 442-760, Republic of Korea. sslee@kyonggi.ac.kr.
authors.forename: Sang-Seob
authors.initials: SS
authors.lastname: Lee
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D011423:Propionibacteriaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012722:Sewage / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage
keywords: Sewage treatment plant; Taxonomy; Tessaracoccus