title: Umbilical Cord-Derived Mesenchymal Stem Cells Relieve Hindlimb Ischemia through Enhancing Angiogenesis in Tree Shrews.
abstract: Hindlimb ischemia is still a clinical problem with high morbidity and mortality. Patients suffer from consequent rest pain, ulcers, cool limbs, and even amputation. Angiogenesis is a promising target for the treatment of ischemic limbs, providing extra blood for the ischemic region. In the present study, we investigated the role of umbilical cord-derived mesenchymal stem cells (UC-MSCs) in regulating angiogenesis and relieving hindlimb ischemia. UC-MSCs were isolated from the umbilical cord of tree shrews. Angiography results showed that UC-MSCs injection significantly promoted angiogenesis in tree shrews. Moreover, the ankle brachial index, transcutaneous oxygen pressure, blood perfusion, and capillary/muscle fiber ratio were all markedly increased by the application of UC-MSCs. In addition, the conditioned culture of human umbilical vein endothelial cells using medium collected from UC-MSCs showed higher expression of angiogenic markers and improved migration ability. In short, the isolated UC-MSCs notably contributed to restoring blood supply and alleviating the symptoms of limb ischemia through enhancing angiogenesis.
authors.affiliation: Department of Vascular Surgery, Kunming General Hospital, Chengdu Military Command, Kunming 650032, China; Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital, Chengdu Military Command, Kunming 650032, China.
authors.forename: Cunping
authors.initials: C
authors.lastname: Yin
authors.affiliation: Department of Geriatrics, Kunming General Hospital, Chengdu Military Command, Kunming 650032, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Medical Imaging, Kunming General Hospital, Chengdu Military Command, Kunming 650032, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital, Chengdu Military Command, Kunming 650032, China.
authors.forename: Guangping
authors.identifier: 0000-0002-3784-7040
authors.initials: G
authors.lastname: Ruan
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital, Chengdu Military Command, Kunming 650032, China.
authors.forename: Zian
authors.initials: Z
authors.lastname: Li
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital, Chengdu Military Command, Kunming 650032, China.
authors.forename: Rongqing
authors.initials: R
authors.lastname: Pang
authors.affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital, Chengdu Military Command, Kunming 650032, China.
authors.forename: Xinghua
authors.identifier: 0000-0002-7954-8911
authors.initials: X
authors.lastname: Pan