title: Preventive Effects of Poloxamer 188 on Muscle Cell Damage Mechanics Under Oxidative Stress.
abstract: High oxidative stress can occur during ischemic reperfusion and chronic inflammation. It has been hypothesized that such oxidative challenges could contribute to clinical risks such as deep tissue pressure ulcers. Skeletal muscles can be challenged by inflammation-induced or reperfusion-induced oxidative stress. Oxidative stress reportedly can lower the compressive damage threshold of skeletal muscles cells, causing actin filament depolymerization, and reduce membrane sealing ability. Skeletal muscles thus become easier to be damaged by mechanical loading under prolonged oxidative exposure. In this study, we investigated the preventive effect of poloxamer 188 (P188) on skeletal muscle cells against extrinsic oxidative challenges (H2O2). It was found that with 1 mM P188 pre-treatment for 1 h, skeletal muscle cells could maintain their compressive damage threshold. The actin polymerization dynamics largely remained stable in term of the expression of cofilin, thymosin beta 4 and profilin. Laser photoporation demonstrated that membrane sealing ability was preserved even as the cells were challenged by H2O2. These findings suggest that P188 pre-treatment can help skeletal muscle cells retain their normal mechanical integrity in oxidative environments, adding a potential clinical use of P188 against the combined challenge of mechanical-oxidative stresses. Such effect may help to prevent deep tissue ulcer development.
authors.affiliation: Division of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, NT, Hong Kong.
authors.forename: Sing Wan
authors.initials: SW
authors.lastname: Wong
authors.affiliation: Division of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, NT, Hong Kong.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Division of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, NT, Hong Kong.
authors.forename: Ye
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Division of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, NT, Hong Kong.
authors.forename: Zhiyao
authors.initials: Z
authors.lastname: Ma
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong.
authors.forename: Stanton H L
authors.initials: SH
authors.lastname: Kok
authors.affiliation: Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, 60607, USA.
authors.forename: Shan
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Cho
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong.
authors.forename: Kenneth K H
authors.initials: KK
authors.lastname: Lee
authors.affiliation: Division of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, NT, Hong Kong. arthurmak@cuhk.edu.hk.
authors.forename: Arthur F T
authors.initials: AF
authors.lastname: Mak
mesh_terms: D000818:Animals; D002460:Cell Line; D019245:Compressive Strength / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000378:metabolism* / Q000473:pathology; D009124:Muscle Proteins / Q000096:biosynthesis*; D018384:Oxidative Stress / Q000187:drug effects*; D020442:Poloxamer / Q000494:pharmacology*
chemical_list: D009124:Muscle Proteins; D020442:Poloxamer; D006861:Hydrogen Peroxide
keywords: Cell mechanics; Deep tissue injury; Oxidative stress; Poloxamer 188; Skeletal muscle