title: Early Subchondral Bone Loss at Arthritis Onset Predicted Late Arthritis Severity in a Rat Arthritis Model.
abstract: Synovitis is usually observed before loss of articular function in rheumatoid arthritis (RA). In addition to the synovium and according to the "Inside-Outside" theory, bone compartment is also involved in RA pathogenesis. Then, we investigated time dependent articular bone loss and prediction of early bone loss to late arthritis severity on the rat adjuvant-induced arthritis (AIA) model. Lewis female rats were longitudinally monitored from arthritis induction (day 0), with early (day 10) and late (day 17) steps. Trabecular and cortical microarchitecture parameters of four ankle bones were assessed by microcomputed tomography. Gene expression was determined at sacrifice. Arthritis occurred at day 10 in AIA rats. At this time, bone erosions were detected on four ankle bones, with cortical porosity increase (+67%) and trabecular alterations including bone volume fraction (BV/TV: -13%), and trabecular thickness decrease. Navicular bone assessment was the most reproducible and sensitive. Furthermore, strong correlations were observed between bone alterations at day 10 and arthritis severity or bone loss at day 17, including predictability of day 10 BV/TV to day 17 articular index (R2= 0.76). Finally, gene expression at day 17 confirmed massive osteoclast activation and interestingly provided insights on strong activation of bone formation inhibitor markers at the joint level. In rat AIA, bone loss was already observed at synovitis onset and was predicted late arthritis severity. Our results reinforced the key role of subchondral bone in arthritis pathogenesis, in favour to the "Inside-Outside" theory. Mechanisms of bone loss in rat AIA involved resorption activation and formation inhibition changes. J. Cell. Physiol. 232: 1318-1325, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: SAINBIOSE, INSERM U1059, LBTO, Saint-Etienne, France.|University of Lyon, Saint-Etienne, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Courbon
authors.affiliation: SAINBIOSE, INSERM U1059, LBTO, Saint-Etienne, France.|University of Lyon, Saint-Etienne, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Cleret
authors.affiliation: SAINBIOSE, INSERM U1059, LBTO, Saint-Etienne, France.|University of Lyon, Saint-Etienne, France.
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Linossier
authors.affiliation: SAINBIOSE, INSERM U1059, LBTO, Saint-Etienne, France.|University of Lyon, Saint-Etienne, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Vico
authors.affiliation: SAINBIOSE, INSERM U1059, LBTO, Saint-Etienne, France.|University of Lyon, Saint-Etienne, France.|Rheumatology Department, University Hospital of Saint-Etienne, Saint-Etienne, France.
authors.forename: Hubert
authors.initials: H
authors.lastname: Marotte
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000150:complications* / Q000235:genetics / Q000473:pathology*; D016723:Bone Remodeling / Q000235:genetics; D001862:Bone Resorption / Q000150:complications* / Q000235:genetics / Q000473:pathology*; D001842:Bone and Bones / Q000473:pathology; D004195:Disease Models, Animal; D005121:Extremities / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D021621:Imaging, Three-Dimensional; D007249:Inflammation / Q000235:genetics / Q000473:pathology; D011917:Rats, Inbred Lew