title: Chiral Separation of Cytotoxic Flavan Derivatives from Daphne giraldii.
abstract: Twelve new flavan derivatives including four pairs of enantiomers, daphnegiralins A1-A4 (1) and daphnegiralins B1-B4 (2), and two pairs of epimers, daphnegiralins C1/C2 (3) and daphnegiralins D1/D2 (4), were isolated from the stem bark and roots of Daphne giraldii. Their structures were elucidated using spectroscopic analyses, computational approaches, and chemical methods. Separation of the enantiomeric mixtures (1a, 1b, 2a, and 2b) was achieved using chiral HPLC. The compounds were evaluated against a small panel of human cancer cell lines, and 1b-2, 2a, and 2b were cytotoxic against Hep3B human hepatoma cells.
authors.affiliation: Department of Natural Products Chemistry, ‡Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, and ⊥School of Pharmaceutical Engineering, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Li
authors.affiliation: Department of Natural Products Chemistry, ‡Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, and ⊥School of Pharmaceutical Engineering, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Natural Products Chemistry, ‡Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, and ⊥School of Pharmaceutical Engineering, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Natural Products Chemistry, ‡Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, and ⊥School of Pharmaceutical Engineering, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Sen
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Natural Products Chemistry, ‡Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, and ⊥School of Pharmaceutical Engineering, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lin
authors.affiliation: Department of Natural Products Chemistry, ‡Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, and ⊥School of Pharmaceutical Engineering, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Chun-Ting
authors.initials: CT
authors.lastname: Liu
authors.affiliation: Department of Natural Products Chemistry, ‡Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, and ⊥School of Pharmaceutical Engineering, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Li
authors.affiliation: Department of Natural Products Chemistry, ‡Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, and ⊥School of Pharmaceutical Engineering, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Huang
authors.affiliation: Department of Natural Products Chemistry, ‡Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, and ⊥School of Pharmaceutical Engineering, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Shao-Jiang
authors.initials: SJ
authors.lastname: Song
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D032364:Daphne / Q000737:chemistry; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013237:Stereoisomerism
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D059808:Polyphenols