title: Identification and statistical optimization of fermentation conditions for a newly isolated extracellular cholesterol oxidase-producing Streptomyces cavourensis strain NEAE-42.
abstract: Due to broad range of clinical and industrial applications of cholesterol oxidase, isolation and screening of bacterial strains producing extracellular form of cholesterol oxidase is of great importance.
  
  
  
  One hundred and thirty actinomycete isolates were screened for their cholesterol oxidase activity. Among them, a potential culture, strain NEAE-42 is displayed the highest extracellular cholesterol oxidase activity. It was selected and identified as Streptomyces cavourensis strain NEAE-42. The optimization of different process parameters for cholesterol oxidase production by Streptomyces cavourensis strain NEAE-42 using Plackett-Burman experimental design and response surface methodology was carried out. Fifteen variables were screened using Plackett-Burman experimental design. Cholesterol, initial pH and (NH4)2SO4 were the most significant positive independent variables affecting cholesterol oxidase production. Central composite design was chosen to elucidate the optimal concentrations of the selected process variables on cholesterol oxidase production. It was found that, cholesterol oxidase production by Streptomyces cavourensis strain NEAE-42 after optimization process was 20.521U/mL which is higher than result obtained from the basal medium before screening process using Plackett-Burman (3.31 U/mL) with a fold of increase 6.19.
  
  
  
  The cholesterol oxidase level production obtained in this study (20.521U/mL) by the statistical method is higher than many of the reported values.
authors.affiliation: Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, Alexandria, 21934, Egypt. nouraelahmady@yahoo.com.
authors.forename: Noura El-Ahmady
authors.initials: Nel-A
authors.lastname: El-Naggar
authors.affiliation: Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, Alexandria, 21934, Egypt.
authors.forename: Nancy M
authors.initials: NM
authors.lastname: El-Shweihy
authors.affiliation: Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, Alexandria, 21934, Egypt.
authors.forename: Sara M
authors.initials: SM
authors.lastname: El-Ewasy
mesh_terms: D000645:Ammonium Sulfate / Q000378:metabolism / Q000494:pharmacology; D000704:Analysis of Variance; D001426:Bacterial Proteins / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002784:Cholesterol / Q000378:metabolism / Q000494:pharmacology; D002789:Cholesterol Oxidase / Q000378:metabolism*; D002965:Classification; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial; D004789:Enzyme Activation / Q000187:drug effects; D057075:Enzyme Assays; D005285:Fermentation; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002784:Cholesterol; D002789:Cholesterol Oxidase; D000645:Ammonium Sulfate
keywords: 16S rRNA; Central composite design; Cholesterol oxidase; Identification; Plackett-Burman design; Streptomyces cavourensis strain NEAE-42