title: Comparative genomic analysis between newly sequenced Brucella suis Vaccine Strain S2 and the Virulent Brucella suis Strain 1330.
abstract: Brucellosis is a bacterial disease caused by Brucella infection. In the late fifties, Brucella suis vaccine strain S2 with reduced virulence was obtained by serial transfer of a virulent B. suis biovar 1 strain in China. It has been widely used for vaccination in China since 1971. Until now, the mechanisms underlie virulence attenuation of S2 are still unknown.
  
  
  
  In this paper, the whole genome sequencing of S2 was carried out by Illumina Hiseq2000 sequencing method. We further performed the comparative genomic analysis to find out the differences between S2 and the virulent Brucella suis strain 1330. We found premature stops in outer membrane autotransporter omaA and eryD genes. Single mutations were found in phosphatidylcholine synthase, phosphorglucosamine mutase, pyruvate kinase and FliF, which have been reported to be related to the virulence of Brucella or other bacteria. Of the other different proteins between S2 and 1330, such as Omp2b, periplasmic sugar-binding protein, and oligopeptide ABC transporter, no definitive implications related to bacterial virulence were found, which await further investigation.
  
  
  
  The data presented here provided the rational basis for designing Brucella vaccines that could be used in other strains.
authors.affiliation: Laboratory of Zoonoses, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Di
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Beijing, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Laboratory of Zoonoses, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Tian
authors.affiliation: Laboratory of Zoonoses, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
authors.forename: Jing-Li
authors.initials: JL
authors.lastname: Kang
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Beijing, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Xinjiang Academy of Animal Science, Institute of Veterinary Research, Urumuqi, Xinjiang, China.
authors.forename: Xin-Ping
authors.initials: XP
authors.lastname: Yi
authors.affiliation: Xinjiang Academy of Animal Science, Institute of Veterinary Research, Urumuqi, Xinjiang, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ye
authors.affiliation: Xinjiang Academy of Animal Science, Institute of Veterinary Research, Urumuqi, Xinjiang, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhong
authors.affiliation: Laboratory of Zoonoses, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ni
authors.affiliation: ZhongXin Biotechology Shanghai Co, Ltd. 12F, Building 1, 100 Qinzhou Road, Shanghai, China.
authors.forename: You-Yu
authors.initials: YY
authors.lastname: He
authors.affiliation: ZhongXin Biotechology Shanghai Co, Ltd. 12F, Building 1, 100 Qinzhou Road, Shanghai, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xia
authors.affiliation: ZhongXin Biotechology Shanghai Co, Ltd. 12F, Building 1, 100 Qinzhou Road, Shanghai, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Beijing, China. cuibuyun@icdc.cn.
authors.forename: Bu-Yun
authors.initials: BY
authors.lastname: Cui
authors.affiliation: Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Shanghai, China. xmao@shvri.ac.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Mao
authors.affiliation: Laboratory of Zoonoses, China Animal Health and Epidemiology Center, Qingdao, Shandong, China. fwxsjl@126.com.
authors.forename: Wei-Xing
authors.initials: WX
authors.lastname: Fan
mesh_terms: D002004:Brucella Vaccine / Q000235:genetics*; D041021:Brucella suis / Q000235:genetics* / Q000472:pathogenicity; D002876:Chromosomes, Bacterial; D055028:Comparative Genomic Hybridization; D019295:Computational Biology / Q000379:methods; D023061:Gene Order; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D020641:Polymorphism, Single Nucleotide; D014774:Virulence / Q000235:genetics
chemical_list: D002004:Brucella Vaccine
keywords: Brucella suis vaccine strain S2; Brucella suis virulent strain 1330; Comparative genomic analysis; Genome sequence