title: Differential protein abundance of a basolateral MCT1 transporter in the human gastrointestinal tract.
abstract: Bacterially derived short chain fatty acids (SCFAs), such as butyrate, are vital in maintaining the symbiotic relationship that exists between humans and their gastrointestinal microbial populations. A key step in this process is the transport of SCFAs across colonic epithelial cells via MCT1 transporters. This study investigated MCT1 protein abundance in various human intestinal tissues. Initial RT-PCR analysis confirmed the expected MCT1 RNA expression pattern of colon > small intestine > stomach. Using surgical resection samples, immunoblot analysis detected higher abundance of a 45 kDa MCT1 protein in colonic tissue compared to ileum tissue (P < 0.001, N = 4, unpaired t-test). Importantly, MCT1 abundance was found to be significantly lower in sigmoid colon compared to ascending colon (P < 0.01, N = 8-11, ANOVA). Finally, immunolocalization studies confirmed MCT1 to be abundant in the basolateral membranes of surface epithelial cells of the ascending, transverse, and descending colon, but significantly less prevalent in the sigmoid colon (P < 0.05, N = 5-21, ANOVA). In conclusion, these data confirm that basolateral MCT1 protein abundance is correlated to levels of bacterially derived SCFAs along the human gastrointestinal tract. These findings highlight the importance of precise tissue location in studies comparing colonic MCT1 abundance between normal and diseased states.
authors.affiliation: School of Biology and Environmental Science, Science Centre West, University College Dublin, Room 2.55, Belfield, Dublin 4, Ireland.
authors.forename: Hashemeya
authors.initials: H
authors.lastname: Al-Mosauwi
authors.affiliation: Institute for Clinical Outcomes Research and Education, St.Vincent's University Hospital, Dublin, Ireland.|College of Life Sciences and Conway Institute of Biomedical and Biomolecular Science, University College Dublin, Dublin, Ireland.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ryan
authors.affiliation: School of Biology and Environmental Science, Science Centre West, University College Dublin, Room 2.55, Belfield, Dublin 4, Ireland.
authors.forename: Alison
authors.initials: A
authors.lastname: McGrane
authors.affiliation: School of Biology and Environmental Science, Science Centre West, University College Dublin, Room 2.55, Belfield, Dublin 4, Ireland.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Riveros-Beltran
authors.affiliation: School of Biology and Environmental Science, Science Centre West, University College Dublin, Room 2.55, Belfield, Dublin 4, Ireland.
authors.forename: Caragh
authors.initials: C
authors.lastname: Walpole
authors.affiliation: School of Biology and Environmental Science, Science Centre West, University College Dublin, Room 2.55, Belfield, Dublin 4, Ireland.
authors.forename: Eugene
authors.initials: E
authors.lastname: Dempsey
authors.affiliation: Institute for Clinical Outcomes Research and Education, St.Vincent's University Hospital, Dublin, Ireland.|College of Life Sciences and Conway Institute of Biomedical and Biomolecular Science, University College Dublin, Dublin, Ireland.
authors.forename: Danielle
authors.initials: D
authors.lastname: Courtney
authors.affiliation: Institute for Clinical Outcomes Research and Education, St.Vincent's University Hospital, Dublin, Ireland.|College of Life Sciences and Conway Institute of Biomedical and Biomolecular Science, University College Dublin, Dublin, Ireland.
authors.forename: Naomi
authors.initials: N
authors.lastname: Fearon
authors.affiliation: Institute for Clinical Outcomes Research and Education, St.Vincent's University Hospital, Dublin, Ireland.
authors.forename: Desmond
authors.initials: D
authors.lastname: Winter
authors.affiliation: College of Life Sciences and Conway Institute of Biomedical and Biomolecular Science, University College Dublin, Dublin, Ireland.
authors.forename: Alan
authors.initials: A
authors.lastname: Baird
authors.affiliation: School of Biology and Environmental Science, Science Centre West, University College Dublin, Room 2.55, Belfield, Dublin 4, Ireland.
authors.forename: Gavin
authors.initials: G
authors.lastname: Stewart
mesh_terms: D003106:Colon / Q000166:cytology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D041981:Gastrointestinal Tract / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D007082:Ileum / Q000166:cytology / Q000378:metabolism; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism*; D000074011:Peptide Transporter 1; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D027501:Monocarboxylic Acid Transporters; D000074011:Peptide Transporter 1; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1
keywords: MCT1; colon; immunolocalization; protein abundance