title: LncRNAs BCYRN1 promoted the proliferation and migration of rat airway smooth muscle cells in asthma via upregulating the expression of transient receptor potential 1.
abstract: BACKGROUND
  Long noncoding RNAs (lncRNAs) played important roles in several biological processes through regulating the expression of protein. However, the function of lncRNA BCYRN1 in airway smooth muscle cells (ASMCs) has not been reported.
  
  
  METHODS
  Male Sprague-Dawley (SD) rats were divided into control and asthma groups and the ovalbumin (OVA) model was constructed. The expression of BCYRN1 and transient receptor potential 1 (TRPC1) were detected in the ASMCs separated from these rats. Then 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-1) assay, Roche real-time cell analyzer (RTCA) DP assay and Transwell cell migration assay were performed to detect the effect of BCYRN1 on the viability/proliferation and migration of ASMCs. RNA pull-down assays and RNA immunoprecipitation assay were used to identify and verify the binding between BCYRN1 and TRPC1. Inspiratory resistance and expiratory resistance were measured in OVA challenged rats with BCYRN1 knockdown.
  
  
  RESULTS
  We foundthe high expression of BCYRN1 and TRPC1 in asthma groups and ASMCs treated with PDGF-BB. Overexpression of BCYRN1 greatly promoted the proliferation and migration of ASMCs. In addition,TRPC1 overexpression reversed the function of si-BCYRN1 indecreasing the viability/proliferation and migration of ASMCs treated with PDGF-BB. BCYRN1 could up-regulate the protein level of TRPC1 through increasing the stability of TRPC1. Finally, we found that BCYRN1 knockdown reduced the inspiratory resistance and expiratory resistance in OVA challenged rats.
  
  
  CONCLUSIONS
  Our study indicated that BCYRN1 promotedthe proliferation and migration of rat ASMCs in asthma via upregulating the expression of TRPC1.
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University Zhengzhou 450003, People's Republic of China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University Zhengzhou 450003, People's Republic of China.
authors.forename: Luo-Xian
authors.initials: LX
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University Zhengzhou 450003, People's Republic of China.
authors.forename: Cui-Jie
authors.initials: CJ
authors.lastname: Tian
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University Zhengzhou 450003, People's Republic of China.
authors.forename: Xue-Yi
authors.initials: XY
authors.lastname: Tang
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University Zhengzhou 450003, People's Republic of China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University Zhengzhou 450003, People's Republic of China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Guo
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University Zhengzhou 450003, People's Republic of China.
authors.forename: Dong-Jun
authors.initials: DJ
authors.lastname: Cheng
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University Zhengzhou 450003, People's Republic of China.
authors.forename: Xian-Liang
authors.initials: XL
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University Zhengzhou 450003, People's Republic of China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Ma
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University Zhengzhou 450003, People's Republic of China.
authors.forename: Zhuo-Chang
authors.initials: ZC
authors.lastname: Chen
keywords: BCYRN1; airway smooth muscle cells; asthma; migration; proliferation; transient receptor potential 1