title: Establishment of Simple and Routine Methods in Early Diagnosis of Gentamicin-Induced Kidney Injury Based on a Rat Model.
abstract: The changes in biomarkers of gentamycin- (GM-) induced kidney injury have been studied by using simple and routine methods and also assessed the efficacy and utility of these routine biomarkers in early diagnosis. Eighty Sprague-Dawley (SD) rats were randomly divided into 4 groups: three experimental groups treated with different GM dosages (4, 20, and 100 mg·kg(-1)) and a control group. The experimental groups were given intramuscular GM injections once daily for 14 days, and the control group was given intramuscular sterile water. Blood and urine samples were collected on treatment days 1, 3, 7, and 14 to test for total protein (TP), albumin (ALB), blood urea nitrogen (BUN), creatinine (CRE), uric acid (UA), pH, specific gravity (SG), proteins (PRO), and cells in urinary sediment. Histopathology and kidney coefficient were performed on excised kidney specimens. The result indicated that serum CRE, BUN, and TP, urine PRO, and urinary hyaline casts and low-transitional epithelium showed an immediate and highly sensitive response to kidney injury, and the combined diagnosis with the above methods could be used in early diagnosis. Particularly, the process of the test was simple and quick, no special equipment, so it is more suit for primary medical institution.
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Cuiyan
authors.identifier: 0000-0002-5159-0712
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Youxi
authors.initials: Y
authors.lastname: Kang
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Long
authors.identifier: 0000-0002-0947-8129
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
authors.forename: Chunyang
authors.identifier: 0000-0003-3191-3884
authors.initials: C
authors.lastname: Han
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001835:Body Weight / Q000187:drug effects; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004195:Disease Models, Animal; D060766:Drinking Water; D005247:Feeding Behavior / Q000187:drug effects; D005839:Gentamicins / Q000009:adverse effects*; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D007674:Kidney Diseases / Q000097:blood / Q000139:chemically induced* / Q000175:diagnosis* / Q000652:urine; D008297:Male; D011507:Proteinuria / Q000097:blood / Q000150:complications / Q000652:urine; D017207:Rats, Sprague-Dawley; D013046:Specific Gravity
chemical_list: D015415:Biomarkers; D060766:Drinking Water; D005839:Gentamicins