title: Delta-aminolevulinate dehydratase activity and oxidative stress markers in preeclampsia.
abstract: Preeclampsia is an important pregnancy-specific multisystem disorder characterized by the onset of hypertension and proteinuria. It is of unknown etiology and involves serious risks for the pregnant women and fetus. One of the main factors involved in the pathophysiology of preeclampsia is oxidative stress, where excess free radicals produce harmful effects, including damage to macromolecules such as lipids, proteins and DNA. In addition, the sulfhydryl delta-aminolevulinate dehydratase enzyme (δ-ALA-D) that is part of the heme biosynthetic pathway in pro-oxidant conditions can be inhibited, which may result in the accumulation of 5-aminolevulinic acid (ALA), associated with the overproduction of free radicals, suggesting it to be an indirect marker of oxidative stress. As hypertensive pregnancy complications are a major cause of morbidity and mortality maternal and fetal where oxidative stress appears to be an important factor involved in preeclampsia, the aim of this study was to evaluate the activity of δ-ALA-D and classic oxidative stress markers in the blood of pregnant women with mild and severe preeclampsia. The analysis and quantification of the following oxidative stress markers were performed: thiobarbituric acid-reactive species (TBARS); presence of protein and non-protein thiol group; quantification of vitamin C; Catalase and δ-ALA--D activities in samples of blood of pregnant women with mild preeclampsia (n=25), with severe preeclampsia (n=30) and in a control group of healthy pregnant women (n=30). TBARS was significantly higher in women with preeclampsia, while the presence of thiol groups, levels of vitamin C, catalase and δ-ALA-D activity were significantly lower in groups of pregnant women with preeclampsia compared with healthy women. In addition, the results showed no significant difference between groups of pregnant women with mild and severe preeclampsia. The data suggest a state of increased oxidative stress in pregnant women with preeclampsia compared to healthy pregnant women, which may be related to the complications of this disease.
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Department of Clinical and Toxicology Analysis, Center of Healthy Sciences, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900, Brazil.
authors.forename: Leidiane
authors.initials: L
authors.lastname: de Lucca
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Department of Clinical and Toxicology Analysis, Center of Healthy Sciences, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900, Brazil.
authors.forename: Fabiane
authors.initials: F
authors.lastname: Rodrigues
authors.affiliation: Department of Clinical and Toxicological Analysis, Center of Health Sciences, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900, Brazil.
authors.forename: Letícia B
authors.initials: LB
authors.lastname: Jantsch
authors.affiliation: Department of Clinical and Toxicological Analysis, Center of Health Sciences, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900, Brazil.
authors.forename: Helena
authors.initials: H
authors.lastname: Kober
authors.affiliation: Department of Obstetrics and Gynecology, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900 Brazil.
authors.forename: Walter S
authors.initials: WS
authors.lastname: Neme
authors.affiliation: Department of Obstetrics and Gynecology, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900 Brazil.
authors.forename: Francisco M P
authors.initials: FMP
authors.lastname: Gallarreta
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Department of Clinical and Toxicology Analysis, Center of Healthy Sciences, Federal University of Santa Maria (UFSM), Santa Maria, 97105-900, Brazil. Electronic address: thissianegoncalves@yahoo.com.br.
authors.forename: Thissiane L
authors.initials: TL
authors.lastname: Gonçalves
mesh_terms: D000328:Adult; D001205:Ascorbic Acid / Q000097:blood; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D015536:Down-Regulation; D005260:Female; D006801:Humans; D018384:Oxidative Stress; D000623:Porphobilinogen Synthase / Q000097:blood*; D011225:Pre-Eclampsia / Q000097:blood* / Q000175:diagnosis / Q000201:enzymology; D011247:Pregnancy; D012720:Severity of Illness Index; D013438:Sulfhydryl Compounds / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D055815:Young Adult
chemical_list: D015415:Biomarkers; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D000623:Porphobilinogen Synthase; D001205:Ascorbic Acid
keywords: Delta-aminolevulinate dehydratase; Oxidative stress; Preeclampsia; Pregnant women