title: Retrospective analysis of oncogenic human papilloma virus and Epstein-Barr virus prevalence in Turkish nasopharyngeal cancer patients.
abstract: Nasopharyngeal carcinoma (NPC) is associated with the Epstein-Barr virus (EBV). Human papilloma virus (HPV) has also been detected in NPC cases. In this retrospective study, we analyze the frequency of EBV and HPV infection in 82 Turkish patients with NPC. A total of 82 were evaluated for EBV and HPV. In situ hybridization (ISH) was performed for EBV. HPV-ISH and P16 immunohistochemistry used to determine the HPV status. Seventy-two of the 82 (87%) NPC patients were EBV-positive. The highest rate of EBV-positivity was found in undifferentiated NPC patients, which accounted for 65 of 68 (95.6%) undifferentiated cases. One of the 82 NPC patients whose tumor was non-keratinizing differentiated, contained HPV. Our data shows that EBV is closely associated with NPC in Turkey. We found lower rates of HPV-positivity in NPC patients than in North American populations. In addition, both EBV and HPV-negativity were more associated with decreased survival than EBV-positive cases.
authors.affiliation: Ankara Atatürk Education and Research Hospital, Department of Pathology, Üniversiteler Mahallesi Bilkent Caddesi No: 1, 06800, Çankaya, Ankara, Turkey. Electronic address: hayriyetatli@gmail.com.
authors.forename: Hayriye
authors.initials: H
authors.lastname: Tatlı Doğan
authors.affiliation: Yıldırım Beyazıt University, Ankara Atatürk Education and Research Hospital, Department of Pathology, Üniversiteler Mahallesi Bilkent Caddesi No: 1, 06800, Çankaya, Ankara, Turkey. Electronic address: dr.kilicaydan@gmail.com.
authors.forename: Aydan
authors.initials: A
authors.lastname: Kılıçarslan
authors.affiliation: Dr. Abdurrahman Yurtaslan Ankara Oncology Education and Research Hospital, Department of Pathology, Mehmet Akif Ersoy Mahallesi 13. Cadde No: 56, 06200, Yenimahalle, Ankara, Turkey. Electronic address: drmehmetdogan@gmail.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Doğan
authors.affiliation: Yıldırım Beyazıt University, Ankara Atatürk Education and Research Hospital, Department of Pathology, Üniversiteler Mahallesi Bilkent Caddesi No: 1, 06800, Çankaya, Ankara, Turkey. Electronic address: nuransungu@gmail.com.
authors.forename: Nuran
authors.initials: N
authors.lastname: Süngü
authors.affiliation: Hacettepe University, Department of Pathology, 06100, Sıhhiye, Ankara, Turkey. Electronic address: gayejp@yahoo.com.
authors.forename: Gaye
authors.initials: G
authors.lastname: Güler Tezel
authors.affiliation: Hacettepe University, Department of Pathology, 06100, Sıhhiye, Ankara, Turkey. Electronic address: gulnurguler@yahoo.com.
authors.forename: Gülnur
authors.initials: G
authors.lastname: Güler
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma; D020031:Epstein-Barr Virus Infections / Q000453:epidemiology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000401:mortality / Q000821:virology*; D030361:Papillomavirus Infections / Q000453:epidemiology*; D015995:Prevalence; D012189:Retrospective Studies; D014421:Turkey; D055815:Young Adult
keywords: EBV; HPV; In situ hybridization; Nasopharynx carcinoma; Turkish patients