title: MiR-285 targets P450 (CYP6N23) to regulate pyrethroid resistance in Culex pipiens pallens.
abstract: MicroRNAs play critical roles in post-transcriptional regulation of gene expression, which participate in the modulation of almost all of the cellular processes. Although emerging evidence indicates that microRNAs are related with antineoplastic drugs resistance, whether microRNAs are responsible for insecticide resistance in mosquitos is poorly understood. In this paper, we found that miR-285 was significantly upregulated in the deltamethrin-resistant strain of Culex pipiens pallens, and overexpression miR-285 through microinjection increased mosquito survival rate against deltamethrin treatement. Using bioinformatic software, quantitative reverse transcription PCR, luciferase reporter assay and microinjection approaches, we conformed that CYP6N23 was the target of miR-285. Lower expression of CYP6N23 was observed in the deltamethrin-resistant strain. While, mosquito mortality rate was decreased after downregulating expression of CYP6N23 by dsRNA against CYP6N23 or miR-285 mimic microinjection. These findings revealed that miR-285 could target CYP6N23 to regulate pyrethroid resistance, providing new insights into mosquito insecticide resistance surveillance and control.
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Tian
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Bingqian
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Xixi
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Zou
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Xianmiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Mengxue
authors.initials: M
authors.lastname: Hu
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Juxin
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China. shenbo@njmu.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, China. clzhu@njmu.edu.cn.
authors.forename: Changliang
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000818:Animals; D003465:Culex / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D000072472:Cytochrome P450 Family 6 / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D011722:Pyrethrins / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D035683:MicroRNAs; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D000072472:Cytochrome P450 Family 6
keywords: CYP6N23; Culex pipiens pallens; Deltamethrin; Insecticide resistance; MiR-285; MicroRNA