title: Rapid and not culture-dependent assay based on multiplex PCR-SSR analysis for monitoring inoculated yeast strains in industrial wine fermentations.
abstract: Wine industry needs a simple method for rapid diagnosis of the dominance of inoculated strains that could be performed routinely during the fermentation process. We present a suitable, high-throughput, and low-cost method to monitor rapidly the dominance of inoculated yeast strains in industrial fermentations of red and white wines using an activated carbon cleaning pretreatment, and a rapid DNA extraction method plus multiplex PCR-SSR analysis. We apply this technique directly to samples of fermenting wines without previously isolating yeast colonies. Results are obtained in a maximum time of 4.5 h.
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, 11510, Puerto Real, Cádiz, Spain. gustavo.cordero@uca.es.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Cordero-Bueso
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, 11510, Puerto Real, Cádiz, Spain.
authors.forename: María Esther
authors.initials: ME
authors.lastname: Rodríguez
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, 11510, Puerto Real, Cádiz, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Garrido
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, 11510, Puerto Real, Cádiz, Spain.
authors.forename: Jesús Manuel
authors.initials: JM
authors.lastname: Cantoral
mesh_terms: D005285:Fermentation; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D027843:Vitis / Q000378:metabolism / Q000382:microbiology; D014920:Wine / Q000032:analysis / Q000382:microbiology*; D015003:Yeasts / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
keywords: Activated carbon; DNA extraction; Fermentation control; Microsatellite; Saccharomyces cerevisiae