title: [Effects of umbilical cord blood mononuclear cells transplantation via lateral ventricle on the neural apoptosis and the expression of Bax and Bcl-2 proteins in neonatal rats with hypoxic-ischemic brain damage].
abstract: OBJECTIVE
  To explore the effects of umbilical cord blood mononuclear cells (UCBMC) transplantation on the neuronal apoptosis and the expression of Bcl-2 and Bax proteins in neonatal rats with hypoxic-ischemic brain damage (HIBD).
  
  
  METHODS
  Seven-day-old Sprague-Dawley neonatal rats were randomly divided into normal control (N)+normal saline (NS), HIBD+NS, N+UCBMC, and HIBD+UCBMC groups. HIBD model was prepared using the classical Rice-Vannucci method. Twenty-four hours after HIBD, UCBMC were transplanted in the N+UCBMC and HIBD+UCBMC groups. Seven days after transplantation, NeuN/Cleaved-Caspase-3 double immunofluorescence staining and TUNEL methods were used to observe neural apoptosis in the cortex. The expression levels of Bax and Bcl-2 proteins were examined by Western blot analysis.
  
  
  RESULTS
  There were more NeuN+cleaved Caspase-3+DAPI+and TUNEL+DAPI+cells in the HIBD+NS group compared with the N+NS and N+UCBMC groups (P<0.01). There were less NeuN+cleaved Caspase-3+DAPI+and TUNEL+DAPI+cells in the HIBD+UCBMC group compared with the HIBD+NS group (P<0.01). The concentration of Bax protein was higher and that of Bcl-2 proteins was lower in the HIBD+NS group compared with the N+NS and N+UCBMC groups (P<0.01). The concentration of Bax protein in HIBD+UCBMC group was lower than that in the HIBD+NS group (P<0.01). The concentration of Bcl-2 protein was higher compared with the HIBD+NS, N+NS and N+UCBMC groups (P<0.05).
  
  
  CONCLUSIONS
  UCBMC transplantation via lateral ventricle can upregulate the expression of Bcl-2 protein and down-regulate the expression of Bax protein, thus alleviating brain neural apoptosis in neonatal rats with HIBD.
authors.affiliation: Department of Medical Imaging, Weifang Medical University, Weifang, Shandong 261053, China (Wang X-L, Email: wxlpine@163.com.
authors.forename: Shao-Zhen
authors.initials: SZ
authors.lastname: Yan
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Wang
authors.forename: Hai-Yu
authors.initials: HY
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Dong
authors.forename: Yan-Song
authors.initials: YS
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D005260:Female; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008297:Male; D009474:Neurons / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein / Q000032:analysis*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D053148:Caspase 3