title: SmShb, the SH2-Containing Adaptor Protein B of Schistosoma mansoni Regulates Venus Kinase Receptor Signaling Pathways.
abstract: Venus kinase receptors (VKRs) are invertebrate receptor tyrosine kinases (RTKs) formed by an extracellular Venus Fly Trap (VFT) ligand binding domain associated via a transmembrane domain with an intracellular tyrosine kinase (TK) domain. Schistosoma mansoni VKRs, SmVKR1 and SmVKR2, are both implicated in reproductive activities of the parasite. In this work, we show that the SH2 domain-containing protein SmShb is a partner of the phosphorylated form of SmVKR1. Expression of these proteins in Xenopus oocytes allowed us to demonstrate that the SH2 domain of SmShb interacts with the phosphotyrosine residue (pY979) located in the juxtamembrane region of SmVKR1. This interaction leads to phosphorylation of SmShb on tyrosines and promotes SmVKR1 signaling towards the JNK pathway. SmShb transcripts are expressed in all parasite stages and they were found in ovary and testes of adult worms, suggesting a possible colocalization of SmShb and SmVKR1 proteins. Silencing of SmShb in adult S. mansoni resulted in an accumulation of mature sperm in testes, indicating a possible role of SmShb in gametogenesis.
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 -UMR 8204-CIIL-Center for Infection and Immunity of Lille, Lille, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Morel
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 -UMR 8204-CIIL-Center for Infection and Immunity of Lille, Lille, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Vanderstraete
authors.affiliation: Univ. Lille, CNRS, UMR 8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France.
authors.forename: Katia
authors.initials: K
authors.lastname: Cailliau
authors.affiliation: BFS, Institute for Parasitology, Justus-Liebig-University, Giessen, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Hahnel
authors.affiliation: BFS, Institute for Parasitology, Justus-Liebig-University, Giessen, Germany.
authors.forename: Christoph G
authors.initials: CG
authors.lastname: Grevelding
authors.affiliation: Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 -UMR 8204-CIIL-Center for Infection and Immunity of Lille, Lille, France.
authors.forename: Colette
authors.initials: C
authors.lastname: Dissous
mesh_terms: D000818:Animals; D015800:Protozoan Proteins / Q000378:metabolism*; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D012550:Schistosoma mansoni / Q000378:metabolism*; D015398:Signal Transduction; D014981:Xenopus
chemical_list: D015800:Protozoan Proteins; D020794:Receptor Protein-Tyrosine Kinases