title: High sensitive and selective C-reactive protein detection by means of lossy mode resonance based optical fiber devices.
abstract: This work presents the development of high sensitive, selective, fast and reusable C-reactive protein (CRP) aptasensors. This novel approach takes advantage of the utilization of high sensitive refractometers based on Lossy Mode Resonances generated by thin indium tin oxide (ITO) films fabricated onto the planar region of d-shaped optical fibers. CRP selectivity is obtained by means of the adhesion of a CRP specific aptamer chain onto the ITO film using the Layer-by-Layer (LbL) nano-assembly fabrication process. The sensing mechanism relies on resonance wavelength shifts originated by refractive index variations of the aptamer chain in presence of the target molecule. Fabricated devices show high selectivity to CRP when compared with other target molecules, such as urea or creatinine, while maintaining a low detection limit (0.0625mg/L) and fast response time (61s). Additionally, these sensors show a repetitive response for several days and are reusable after a cleaning process in ultrapure water.
authors.affiliation: UPNA Sensors Group, Electrical and Electronic Engineering Department, Public University of Navarra, Edificio de Los Tejos, Campus Arrosadia, 31006 Pamplona, Spain. Electronic address: pablo.zubiate@unavarra.es.
authors.forename: P
authors.initials: P
authors.lastname: Zubiate
authors.affiliation: UPNA Sensors Group, Electrical and Electronic Engineering Department, Public University of Navarra, Edificio de Los Tejos, Campus Arrosadia, 31006 Pamplona, Spain; Institute of Smart Cities, Jeronimo de Ayanz Center, Campus Arrosadia, 31006 Pamplona, Spain.
authors.forename: C R
authors.initials: CR
authors.lastname: Zamarreño
authors.affiliation: UPNA Sensors Group, Electrical and Electronic Engineering Department, Public University of Navarra, Edificio de Los Tejos, Campus Arrosadia, 31006 Pamplona, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Sánchez
authors.affiliation: UPNA Sensors Group, Electrical and Electronic Engineering Department, Public University of Navarra, Edificio de Los Tejos, Campus Arrosadia, 31006 Pamplona, Spain; Institute of Smart Cities, Jeronimo de Ayanz Center, Campus Arrosadia, 31006 Pamplona, Spain.
authors.forename: I R
authors.initials: IR
authors.lastname: Matias
authors.affiliation: UPNA Sensors Group, Electrical and Electronic Engineering Department, Public University of Navarra, Edificio de Los Tejos, Campus Arrosadia, 31006 Pamplona, Spain; Institute of Smart Cities, Jeronimo de Ayanz Center, Campus Arrosadia, 31006 Pamplona, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Arregui
mesh_terms: D015374:Biosensing Techniques; D002097:C-Reactive Protein / Q000737:chemistry / Q000302:isolation & purification*; D005336:Fiber Optic Technology / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D055100:Optical Fibers; D017971:Tin Compounds / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D017971:Tin Compounds; D014867:Water; C109984:indium tin oxide; D002097:C-Reactive Protein
keywords: Biosensors; C-reactive protein; D-shaped; Lossy mode resonance; Optical fiber