title: Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis.
abstract: The activity of cysteine cathepsin B increased markedly in lung homogenates and in bronchoalveolar lavage fluids (BALF) of the mouse model of bleomycin-induced lung fibrosis after 14 days of challenge. In contrast the level of the cysteine cathepsin inhibitor cystatin C was unaffected in BALF of wild-type and cathepsin B-deficient mice. Therefore, murine cystatin C is not a reliable marker of fibrosis during bleomycin-induced lung fibrosis. Current data are in sharp contrast to previous analysis carried on human BALF from patients with idiopathic pulmonary fibrosis, for which the level of cathepsin B remained unchanged while cystatin C was significantly increased.
authors.affiliation: INSERM, UMR 1100/Centre d'Etude des Pathologies Respiratoires (CEPR), Université François Rabelais, Faculté de Médecine, 10 Boulevard Tonnellé, F-37032, Tours, cedex, France.|Present address: Laboratoire de Biologie des Tumeurs et du Développement (LBTD), Université de Liège, Liège, Belgium.
authors.forename: Mariana
authors.initials: M
authors.lastname: Kasabova
authors.affiliation: CNRS, UMR-INEM 7355, Immunologie et Neurogénétique Experimentales et Moléculaires, Université d'Orléans, Orléans, France.
authors.forename: Bérengère
authors.initials: B
authors.lastname: Villeret
authors.affiliation: CNRS, UMR-INEM 7355, Immunologie et Neurogénétique Experimentales et Moléculaires, Université d'Orléans, Orléans, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Gombault
authors.affiliation: INSERM, UMR 1100/Centre d'Etude des Pathologies Respiratoires (CEPR), Université François Rabelais, Faculté de Médecine, 10 Boulevard Tonnellé, F-37032, Tours, cedex, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Lecaille
authors.affiliation: Institute of Molecular Medicine and Cell Research, Medical Faculty, Albert-Ludwigs-University Freiburg, Freiburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Reinheckel
authors.affiliation: INSERM, UMR 1100/Centre d'Etude des Pathologies Respiratoires (CEPR), Université François Rabelais, Faculté de Médecine, 10 Boulevard Tonnellé, F-37032, Tours, cedex, France.|Centre Hospitalier Régional Universitaire Tours, Service de Pneumologie, Tours, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Marchand-Adam
authors.affiliation: CNRS, UMR-INEM 7355, Immunologie et Neurogénétique Experimentales et Moléculaires, Université d'Orléans, Orléans, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Couillin
authors.affiliation: INSERM, UMR 1100/Centre d'Etude des Pathologies Respiratoires (CEPR), Université François Rabelais, Faculté de Médecine, 10 Boulevard Tonnellé, F-37032, Tours, cedex, France. gilles.lalmanach@univ-tours.fr.
authors.forename: Gilles
authors.initials: G
authors.lastname: Lalmanach
keywords: Bleomycin; Bronchoalveolar lavage fluid; Cathepsins; Cystatins; Fibrosis; Lung; Proteases