title: Development of microsatellite markers using next-generation sequencing for the columnar cactus Echinopsis chiloensis (Cactaceae).
abstract: The aim of this study was to develop microsatellite markers as a tool to study population structure, genetic diversity and effective population size of Echinopsis chiloensis, an endemic cactus from arid and semiarid regions of Central Chile. We developed 12 polymorphic microsatellite markers for E. chiloensis using next-generation sequencing and tested them in 60 individuals from six sites, covering all the latitudinal range of this species. The number of alleles per locus ranged from 3 to 8, while the observed (Ho) and expected (He) heterozygosity ranged from 0.0 to 0.80 and from 0.10 to 0.76, respectively. We also detected significant differences between sites, with FSTvalues ranging from 0.05 to 0.29. Microsatellite markers will enable us to estimate genetic diversity and population structure of E. chiloensis in future ecological and phylogeographic studies.
authors.affiliation: Departamento de Ecología, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, Chile. cossa@bio.puc.cl.|Institute of Ecology and Biodiversity (IEB), Casilla 653, Santiago, Chile. cossa@bio.puc.cl.
authors.forename: Carmen G
authors.initials: CG
authors.lastname: Ossa
authors.affiliation: Research Group Spermatophytes, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium.|Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AE, UK.
authors.forename: Isabel
authors.initials: I
authors.lastname: Larridon
authors.affiliation: Departamento de Ecología, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, Chile.
authors.forename: Gioconda
authors.initials: G
authors.lastname: Peralta
authors.affiliation: Research Group Spermatophytes, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium.
authors.forename: Pieter
authors.initials: P
authors.lastname: Asselman
authors.affiliation: Departamento de Ecología, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, Chile.|Institute of Ecology and Biodiversity (IEB), Casilla 653, Santiago, Chile.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Pérez
mesh_terms: D029421:Cactaceae / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D059014:High-Throughput Nucleotide Sequencing / Q000592:standards*; D018895:Microsatellite Repeats; D010802:Phylogeny; D012015:Reference Standards; D017422:Sequence Analysis, DNA / Q000592:standards*
chemical_list: D018744:DNA, Plant
keywords: Cactaceae; Echinopsis chiloensis; Population genetics; Simple sequence repeat (SSR) marker