title: triSPIM: light sheet microscopy with isotropic super-resolution.
abstract: We propose a three-objective light sheet microscopy geometry which, through a combination of skewed lattice light sheet excitation through two objectives and the computational fusion of images taken from two separate lens pairings, would allow for isotropic super-resolution in mesoscopic samples. We also show that simultaneous coherent excitation through two excitation objectives could further substantially increase resolution. Simulations demonstrate that our design could achieve a resolution of 120 nm for EGFP imaging while minimizing photodamage.
authors.forename: James D
authors.initials: JD
authors.lastname: Manton
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Rees