title: Reprint of "Mixed-mode chromatography in pharmaceutical and biopharmaceutical applications".
abstract: Mixed-mode chromatography (MMC) is a fast growing area in recent years, thanks to the new generation of mixed-mode stationary phases and better understanding of multimode interactions. MMC has superior applications in the separation of compounds that are not retained or not well resolved by typical reversed-phase LC methods, especially for polar and charged molecules. Due to the multiple retention modes that a single MMC column can offer, often MMC provides additional dimension to a separation method by adjusting the mobile phase conditions. Mixed-mode media is also an effective way to clean up complex sample matrices for purification purposes or for sensitive detection of trace amounts of analytes. In this article, we discuss mixed-mode stationary phases and separation mechanisms and review recent advances in pharmaceutical and biopharmaceutical applications including the analysis and/or purification of counterions, small molecule drugs, impurities, formulation excipients, peptides and proteins.
authors.affiliation: Genentech, 1 DNA Way, South San Francisco, CA 94080, United States. Electronic address: zhang.kelly@gene.com.
authors.forename: Kelly
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Thermo Fisher Scientific, 1228 Titan Way, Sunnyvale, CA 94088, United States.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Liu
mesh_terms: D001702:Biopharmaceutics / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry
chemical_list: D004364:Pharmaceutical Preparations
keywords: Biopharmaceutics; Mixed-mode chromatography; Pharmaceutics