title: MiR-24-3p enhances cell growth in hepatocellular carcinoma by targeting metallothionein 1M.
abstract: Dysregulation of microRNAs has been demonstrated to contribute to malignant progression of cancers, including hepatocellular carcinoma (HCC). MiR-24-3p was previously reported to be significantly upregulated in HCC. However, the potential role and mechanism of action of miR-24-3p in the initiation and progression of HCC remain largely unknown. Quantitative reverse transcription polymerase chain reaction demonstrated that miR-24-3p was significantly upregulated in HCC tumor tissues compared with nontumor tissues. The cell viability, colony formation assay, and tumorigenicity assays in nude mice showed that miR-24-3p could enhance HCC cell growth in vitro and in vivo. Metallothionein 1M was verified as an miR-24-3p target gene by using dual-luciferase reporter assays, quantitative reverse transcription polymerase chain reaction, and Western blotting, which was involved in miR-24-3p regulated HCC cell growth. These results indicated that miR-24-3p plays an important role in the initiation and progression of HCC by targeting metallothionein 1M, and the miR-24-3p/metallothionein 1M pathway may contribute to the development of novel therapeutic strategies for HCC in the future.
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Cancer Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Cancer Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ding
authors.affiliation: Department of Cancer Center, The first affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Cancer Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yan
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Cancer Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Cancer Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Cancer Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Cancer Research Institute, Cancer Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China. guowj0016@126.com.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Guo
mesh_terms: D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D015854:Up-Regulation / Q000235:genetics
chemical_list: C543845:MIRN24 microRNA, human; C000595829:MT1M protein, human; D035683:MicroRNAs; D008668:Metallothionein
keywords: MT1M; cell growth; hepatocellular carcinoma; miR-24-3p