title: Antioxidant activities of protein hydrolysates obtained from the housefly larvae.
abstract: The housefly is an important resource insect and the housefly larvae are ideal source of food additives. The housefly larvae protein hydrolysates were obtained by enzymatic hydrolysis by alcalase and neutral proteinase. Their antioxidant activities were investigated, including the superoxide and hydroxyl radicalscavenging activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, reducing power and metal chelating activity. The antioxidant activities of both hydrolysates increased with their increasing concentrations. The alcalase hydrolysate (AH) showed higher scavenging activities against hydroxyl radical and superoxide anion radical at low concentrations and higher metal-chelating activity than the neutral proteinase hydrolysate (NPH). The NPH exhibited higher scavenging activity against DPPH free radical and higher reducing power than the AH. Both hydrolysates showed more than 50% superoxide anion radical-scavenging activity at 10 μg/mL. These results indicate that both housefly larvae protein hydrolysates display high antioxidant activities and they could serve as potential natural antioxidant food additives.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences , Beijing 100101 , China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences , Beijing 100101 , China.
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences , Beijing 100101 , China.
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences , Beijing 100101 , China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences , Beijing 100101 , China.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences , Beijing 100101 , China.
authors.forename: Qi-Lian
authors.initials: QL
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences , Beijing 100101 , China.
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Wu
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006793:Houseflies / Q000196:embryology / Q000378:metabolism*; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000737:chemistry; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D007502:Iron Chelating Agents / Q000378:metabolism / Q000494:pharmacology; D007814:Larva / Q000378:metabolism; D008666:Metalloendopeptidases / Q000378:metabolism; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D011492:Protein Hydrolysates / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013381:Subtilisins / Q000378:metabolism; D013481:Superoxides / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D019476:Insect Proteins; D007502:Iron Chelating Agents; D010851:Picrates; D011492:Protein Hydrolysates; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; D013381:Subtilisins; D008666:Metalloendopeptidases
keywords: Housefly; food additive; free radical scavenging activity; protein hydrolysates