title: Transcriptomic comparison between Brassica oleracea and rice (Oryza sativa) reveals diverse modulations on cell death in response to Sclerotinia sclerotiorum.
abstract: Sclerotinia stem rot caused by Sclerotinia sclerotiorum is a devastating disease of Brassica crops, but not in rice. The leaves of a rice line, a partial resistant (R) and a susceptible (S) Brassica oleracea pool that bulked from a resistance-segregating F2 population were employed for transcriptome sequencing before and after inoculation by S. sclerotiorum for 6 and 12 h. Distinct transcriptome profiles were revealed between B. oleracea and rice in response to S. sclerotiorum. Enrichment analyses of GO and KEGG indicated an enhancement of antioxidant activity in the R B. oleracea and rice, and histochemical staining exhibited obvious lighter reactive oxygen species (ROS) accumulation and cell death in rice and the R B. oleracea as compared to that in the S B. oleracea. Significant enhancement of Ca(2+) signalling, a positive regulator of ROS and cell death, were detected in S B. oleracea after inoculation, while it was significantly repressed in the R B. oleracea group. Obvious difference was detected between two B. oleracea groups for WRKY transcription factors, particularly for those regulating cell death. These findings suggest diverse modulations on cell death in host in response to S. sclerotiorum. Our study provides useful insight into the resistant mechanism to S. sclerotiorum.
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Mei
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Yijuan
authors.initials: Y
authors.lastname: Ding
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, P.R. China.
authors.forename: Chaobo
authors.initials: C
authors.lastname: Tong
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Du
authors.affiliation: College of Plant Protection, Southwest University, Chongqing 400716, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Huafang
authors.initials: H
authors.lastname: Wan
authors.affiliation: School of Computer and Information Science, Southwest University, Chongqing 400716, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, P.R. China.
authors.forename: Jingyin
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, P.R. China.
authors.forename: Shengyi
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Jiana
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qian
mesh_terms: D001203:Ascomycota / Q000254:growth & development*; D001937:Brassica / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D016923:Cell Death; D020869:Gene Expression Profiling; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D013045:Species Specificity; D059467:Transcriptome