title: An auto-inducible Escherichia coli strain obtained by adaptive laboratory evolution for fatty acid synthesis from ionic liquid-treated bamboo hydrolysate.
abstract: Adaptive laboratory evolution (ALE) is a useful metabolic engineering strategy, which allows the selection of the microorganisms with beneficial phenotype through accumulative beneficial mutations among genetic variations occurrencely. Following ALE strategy, a rational constructed Escherichia coli strain DQ101 for fatty acids synthesis was adaptively evolved for 90days with increasing [C4mim]Cl concentration from 1% to 7% (w/v). The evolved strain DQ102 reached a final OD600of 4.93 at the end of the 24h culture with 7% (w/v) ionic liquid. DQ102/pDQTES with a thioesterase 'TesA overexpression could produce 1.12g/L fatty acid with a productivity of 0.023g/L-h from ionic liquid-treated bamboo hydrolysate. With another β-hydroxyacyl-ACP dehydratases (fabZ) overexpression, DQ102/pDQTESZ could reach a higher concentration of 2.29g/L with a productivity of 0.048g/L-h. These results indicated that ALE could be implemented as a useful tool for metabolic engineering and production of bio-fuels, as well as commodity and specialty chemicals.
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, PR China; State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, Nanjing 210009, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Qin
authors.affiliation: Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, 11 Cihu Road, Huangshi 435002, PR China.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, Nanjing 210009, PR China.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, PR China; State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, Nanjing 210009, PR China. Electronic address: dwang@cqu.edu.cn.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D060847:Metabolic Engineering / Q000379:methods*
chemical_list: D005227:Fatty Acids
keywords: Adaptive laboratory evolution; Bamboo hydrolysate; Escherichia coli; Fatty acids; Ionic liquid