title: Upregulation of miR-124 by physcion 8-O-β-glucopyranoside inhibits proliferation and invasion of malignant melanoma cells via repressing RLIP76.
abstract: Melanoma is the most malignant type of skin cancer. In recent years, mounting studies have evidenced the involvement of miRNAs in melanoma. One of these miRNAs, miR-124 has been found aberrantly downregulated in a variety of human malignancies. In this study, our results showed that the expression of miR-124 was significantly lower in malignant melanoma tissues and cell lines and miR-124 functioned as a tumor suppressor in melanoma. Moreover, our findings showed that miR-124 exerted anti-tumor effect by directly targeting RLIP76, a stress-inducible non-ABC transporter that plays a crucial role in the development of melanoma. Furthermore, our study also showed that physcion 8-O-β-glucopyranoside, a natural compound from medicinal plant, could inhibit the proliferation and invasion of melanoma cells by targeting miR-124/RLIP76 signaling.
authors.affiliation: Qingdao University, Qingdao, Shandong, 266071, China.
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Qingdao University, Qingdao, Shandong, 266071, China. Electronic address: qdhyt123@163.com.
authors.forename: Yantao
authors.initials: Y
authors.lastname: Han
authors.affiliation: Qingdao University, Qingdao, Shandong, 266071, China. Electronic address: ZD@qdu.edu.cn.
authors.forename: Luo
authors.initials: L
authors.lastname: Xu
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000037:antagonists & inhibitors / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004642:Emodin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D020690:GTPase-Activating Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D008297:Male; D008544:Melanocytes / Q000187:drug effects / Q000378:metabolism; D008545:Melanoma / Q000188:drug therapy / Q000378:metabolism*; D035683:MicroRNAs / Q000096:biosynthesis*; D009005:Monosaccharides / Q000494:pharmacology* / Q000627:therapeutic use; D009361:Neoplasm Invasiveness; D012878:Skin Neoplasms; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology; D000096142:Cutaneous Malignant Melanoma
chemical_list: C543378:8-O-beta-D-4'-deoxy-delta(4')-glucuropyranoside; D018528:ATP-Binding Cassette Transporters; D020690:GTPase-Activating Proteins; C509207:MIRN124 microRNA, human; D035683:MicroRNAs; D009005:Monosaccharides; C095637:RALBP1 protein, human; C008905:physcione; D004642:Emodin
keywords: -glucopyranoside; Melanoma; Physcion 8-O-β; RLIP76; miR-124