title: SCOP/PHLPP1β in the basolateral amygdala regulates circadian expression of mouse anxiety-like behavior.
abstract: While disruption of the circadian clock triggers a spectrum of affective abnormalities, how the clock regulates mammalian emotionality remains unclear. Here, we characterized the time-of-day-dependent regulation of mouse anxiety-like behaviors. We show that anxiety-like behaviors are expressed in a circadian manner in mice and demonstrate that the clock machineries in the dorsal telencephalon (dTel) are required for the time-of-day-dependent regulation of anxiety-like behaviors. We identify suprachiasmatic nucleus circadian oscillatory protein (SCOP/PHLPP1β) as an essential intracellular signaling molecule mediating this temporal regulation downstream of the clock. Using viral-mediated, basolateral amygdala (BLA)-specific knockout of Scop, we demonstrate that deletion of SCOP in the BLA exerts anxiolytic effects on the elevated plus maze at early subjective night, thereby blunting the circadian variation in the anxiety-like behavior. We conclude that the circadian expression of SCOP in the BLA plays a key role in generating circadian rhythmicity in the anxiety-like behavior. Our results demonstrate SCOP as a regulator of anxiety-like behaviors and reveal its key roles in the anxiogenic functions of the BLA.
authors.affiliation: Department of Biological Sciences, School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Jun J
authors.initials: JJ
authors.lastname: Nakano
authors.affiliation: Department of Biological Sciences, School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Shimizu
authors.affiliation: Department of Health Science, School of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274-8555, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Shimba
authors.affiliation: Department of Biological Sciences, School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Fukada
mesh_terms: D000818:Animals; D001007:Anxiety / Q000378:metabolism*; D066272:Basolateral Nuclear Complex / Q000378:metabolism*; D001522:Behavior, Animal; D057906:Circadian Clocks; D002940:Circadian Rhythm; D008297:Male; D018782:Maze Learning; D008810:Mice, Inbred C57BL; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; D012333:RNA, Messenger; C519282:PHLPP1 protein, mouse; D010749:Phosphoprotein Phosphatases