title: Chlorogenic acid prevents isoproterenol-induced DNA damage in vascular smooth muscle cells.
abstract: Numerous clinical therapeutic agents have been identified as DNA damaging. The present study revealed that isoproterenol (Iso) resulted in DNA damage in vascular smooth muscle cells (VSMCs) and increased the levels of intracellular oxygen free radicals. Administration of chlorogenic acid (CGA) inhibited this effect. Pretreatment with CGA abrogated the increase in protein expression levels of γ‑H2A histone family member X, phosphorylated ataxia telangiectasia mutated, phosphorylated Rad3‑related protein, breast cancer 1 and C‑terminal Src homologous kinase induced by Iso. In addition, the increase in levels of intracellular reactive oxygen species (ROS) induced by Iso was inhibited by CGA pretreatment in a dose‑dependent manner. The results of the present study suggest that CGA may inhibit Iso‑induced VSMC damage via the suppression of ROS generation. Therefore, CGA may be a novel agent for the treatment of vascular diseases.
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai First Maternity and Infant Hospital, Shanghai 201204, P.R. China.
authors.forename: Jingshuai
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of General Medicine, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200000, P.R. China.
authors.forename: Jiyang
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Cardiovascular and Thoracic Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital of Tongji University, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors.forename: Mengjiao
authors.initials: M
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital of Tongji University, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Tong
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital of Tongji University, Tongji University School of Medicine, Shanghai 200065, P.R. China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000008:administration & dosage*; D004249:DNA Damage / Q000187:drug effects*; D007545:Isoproterenol / Q000633:toxicity; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000473:pathology; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D002726:Chlorogenic Acid; D007545:Isoproterenol