title: CD82 Is a Marker for Prospective Isolation of Human Muscle Satellite Cells and Is Linked to Muscular Dystrophies.
abstract: Cell-surface markers for prospective isolation of stem cells from human skeletal muscle have been difficult to identify. Such markers would be powerful tools for studying satellite cell function during homeostasis and in pathogenesis of diseases such as muscular dystrophies. In this study, we show that the tetraspanin KAI/CD82 is an excellent marker for prospectively isolating stem cells from human fetal and adult skeletal muscle. Human CD82+muscle cells robustly engraft into a mouse model of muscular dystrophy. shRNA knockdown of CD82 in myogenic cells reduces myoblast proliferation, suggesting it is functionally involved in muscle homeostasis. CD82 physically interacts with alpha7beta1 integrin (α7β1-ITG) and with α-sarcoglycan, a member of the Dystrophin-Associated Glycoprotein Complex (DAPC), both of which have been linked to muscular dystrophies. Consistently, CD82 expression is decreased in Duchenne muscular dystrophy patients. Together, these findings suggest that CD82 function may be important for muscle stem cell function in muscular disorders.
authors.affiliation: Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA; The Stem Cell Program at Boston Children's Hospital, Boston, MA 02115, USA.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Alexander
authors.affiliation: Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA.
authors.forename: Anete
authors.initials: A
authors.lastname: Rozkalne
authors.affiliation: Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA; Boston University School of Medicine, Boston, MA 02215, USA.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Colletta
authors.affiliation: Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA; The Stem Cell Program at Boston Children's Hospital, Boston, MA 02115, USA.
authors.forename: Janelle M
authors.initials: JM
authors.lastname: Spinazzola
authors.affiliation: Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA.
authors.forename: Samuel
authors.initials: S
authors.lastname: Johnson
authors.affiliation: Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA; The Stem Cell Program at Boston Children's Hospital, Boston, MA 02115, USA.
authors.forename: Fedik
authors.initials: F
authors.lastname: Rahimov
authors.affiliation: Division of Pediatric Pathology, Department of Pathology and Laboratory Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Meng
authors.affiliation: Division of Pediatric Pathology, Department of Pathology and Laboratory Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Lawlor
authors.affiliation: Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA.
authors.forename: Elicia
authors.initials: E
authors.lastname: Estrella
authors.affiliation: Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA; The Stem Cell Program at Boston Children's Hospital, Boston, MA 02115, USA; The Manton Center for Orphan Disease Research, Boston Children's Hospital, Boston, MA 02115, USA.
authors.forename: Louis M
authors.initials: LM
authors.lastname: Kunkel
authors.affiliation: Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA; The Stem Cell Program at Boston Children's Hospital, Boston, MA 02115, USA. Electronic address: gussoni@enders.tch.harvard.edu.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Gussoni
mesh_terms: D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D051929:CD146 Antigen / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D039001:Integrin alpha Chains / Q000378:metabolism; D051861:Kangai-1 Protein / Q000378:metabolism*; D016513:Mice, SCID; D009136:Muscular Dystrophies / Q000378:metabolism* / Q000473:pathology*; D009137:Muscular Dystrophy, Animal / Q000473:pathology; D049031:Sarcoglycans / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D051929:CD146 Antigen; D039001:Integrin alpha Chains; D051861:Kangai-1 Protein; C497902:MCAM protein, human; D049031:Sarcoglycans; C085725:integrin alpha7