title: Following the Motion of Polycomb Bodies in Living Drosophila Embryos.
abstract: During the last two decades, observation of cell nuclei in live microscopy evidences motion of nuclear compartments. Drosophila embryos constitute a good model to study nuclear dynamic during cell differentiation because they can easily be observed in live microscopy. Inside the cell nucleus, Polycomb group proteins accumulate in foci named Pc bodies. Here, we describe a method to visualize and analyze the motion of these nuclear compartments inside cell nuclei of Drosophila embryos.
authors.affiliation: Institut de Génétique Humaine, CNRS UPR 1142, 141, rue de la Cardonille, 34396, Montpellier Cedex 5, France. Thierry.cheutin@igh.cnrs.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Cheutin
authors.affiliation: Institut de Génétique Humaine, CNRS UPR 1142, 141, rue de la Cardonille, 34396, Montpellier Cedex 5, France.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Cavalli
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics*; D004330:Drosophila / Q000196:embryology / Q000235:genetics; D004625:Embryo, Nonmammalian; D008853:Microscopy / Q000379:methods*; D063146:Polycomb-Group Proteins / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D063146:Polycomb-Group Proteins
keywords: Drosophila embryo; Live microscopy; Nucleus dynamics; Polycomb bodies