title: Cell Adhesion and Proliferation on Sulfonated and Non-Modified Chitosan Films.
abstract: Three types of chitosan-based films have been prepared and evaluated: a non-modified chitosan film bearing cationizable aliphatic amines and two films made of N-sulfopropyl chitosan derivatives bearing both aliphatic amines and negative sulfonate groups at different ratios. Cell adhesion and proliferation on chitosan films of C2C12 pre-myoblastic cells and B16 cells as tumoral model have been tested. A differential cell behavior has been observed on chitosan films due to their different surface modification. B16 cells have shown lower vinculin expression when cultured on sulfonated chitosan films. This study shows how the interaction among cells and material surface can be modulated by physicochemical characteristics of the biomaterial surface, altering tumoral cell adhesion and proliferation processes.
authors.affiliation: Tissue Engineering Group, Institute of Biofunctional Studies, Pharmacy Faculty, (Associated Unit to the Institute of Polymer Science and Technology (CSIC), Complutense University of Madrid (UCM), Paseo Juan XXIII, 1, Madrid, 28040, Spain. emartinezcampos79@gmail.com.
authors.forename: Enrique
authors.initials: E
authors.lastname: Martínez-Campos
authors.affiliation: Tissue Engineering Group, Institute of Biofunctional Studies, Pharmacy Faculty, (Associated Unit to the Institute of Polymer Science and Technology (CSIC), Complutense University of Madrid (UCM), Paseo Juan XXIII, 1, Madrid, 28040, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Civantos
authors.affiliation: Polymer Functionalization Group, Institute of Polymer Science and Technology, ICTP-CSIC, Juan de la Cierva 3, Madrid, 28006, Spain.
authors.forename: Juan Alfonso
authors.initials: JA
authors.lastname: Redondo
authors.affiliation: Tissue Engineering Group, Institute of Biofunctional Studies, Pharmacy Faculty, (Associated Unit to the Institute of Polymer Science and Technology (CSIC), Complutense University of Madrid (UCM), Paseo Juan XXIII, 1, Madrid, 28040, Spain.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Guzmán
authors.affiliation: Polymer Functionalization Group, Institute of Polymer Science and Technology, ICTP-CSIC, Juan de la Cierva 3, Madrid, 28006, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Pérez-Perrino
authors.affiliation: Polymer Functionalization Group, Institute of Polymer Science and Technology, ICTP-CSIC, Juan de la Cierva 3, Madrid, 28006, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Gallardo
authors.affiliation: Tissue Engineering Group, Institute of Biofunctional Studies, Pharmacy Faculty, (Associated Unit to the Institute of Polymer Science and Technology (CSIC), Complutense University of Madrid (UCM), Paseo Juan XXIII, 1, Madrid, 28040, Spain.
authors.forename: Viviana
authors.initials: V
authors.lastname: Ramos
authors.affiliation: Polymer Functionalization Group, Institute of Polymer Science and Technology, ICTP-CSIC, Juan de la Cierva 3, Madrid, 28006, Spain. iaranaz@hotmail.com.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Aranaz
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D013499:Surface Properties; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D048271:Chitosan
keywords: N-sulfopropyl chitosan; focal adhesion; melanoma; proliferation