title: Preparation and characterization of electrospun alginate/PLA nanofibers as tissue engineering material by emulsion eletrospinning.
abstract: Scaffolds made by biomaterials offer favorite environment for cell grow and show a wide potential application in tissue engineering. Novel biocompatibility materials polylatic acid (PLA) nanofiber membranes with favorable biocompatibility and good mechanical strength could serve as an innovative tissue engineering scaffold. Sodium alginate (SA) could be used in biomedical areas because of its anti-bacterial property, hydrophilicity and biocompatibility. In this article, we chose PLA as continuous phase and SA as dispersion phase to prepare a W/O emulsion and then electrospun it to get a SA/PLA composite nanofiber membranes. The CLSM images illustrated that the existence of SA was located on the surface of composite fibers and the FTIR results confirmed the result. A calcium ion replacement step was used as an after-treatment for SA/PLA nanofiber membranes in order to anchor the alginic ion in a form of gelated calcium alginate (CA). The single fiber tensile test shows a good mechanical property of CA/PLA nanofiber membranes, and the nanofiber membranes are beneficial for cell proliferation and differentiation owing to MTT array as well as Alizarin red S (ARS) staining test.
authors.affiliation: Department of Polymer Materials and Engineering, South China University of Technology, Guangzhou 510640, People׳s Republic of China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Xu
authors.affiliation: The Affiliated Zhongshan Hospital of Xiamen University, People׳s Republic of China.
authors.forename: Renzhe
authors.initials: R
authors.lastname: Shen
authors.affiliation: Department of Polymer Materials and Engineering, South China University of Technology, Guangzhou 510640, People׳s Republic of China. Electronic address: yryan@scut.edu.cn.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Yan
authors.affiliation: The Affiliated Hospital of Guangzhou Medical University, Guangzhou, People׳s Republic of China. Electronic address: 48014713@qq.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
mesh_terms: D000464:Alginates / Q000138:chemical synthesis*; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D020723:Glucuronic Acid / Q000138:chemical synthesis; D006603:Hexuronic Acids / Q000138:chemical synthesis; D057139:Nanofibers; D011091:Polyesters / Q000138:chemical synthesis*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D011091:Polyesters; C033616:poly(lactide); D020723:Glucuronic Acid
keywords: Alginate; Emulsions electrospinning; Polylatic acid; Tissue engineering