title: CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, confers broad-spectrum resistance by activating priming.
abstract: In Arabidopsis, several L-type lectin receptor kinases (LecRKs) have been identified as putative immune receptors. However, to date, there have been few analyses of LecRKs in crop plants. Virus-induced gene silencing of CaLecRK-S.5 verified the role of CaLecRK-S.5 in broad-spectrum resistance. Compared with control plants, CaLecRK-S.5-silenced plants showed reduced hypersensitive response, reactive oxygen species burst, secondary metabolite production, mitogen-activated protein kinase activation, and defense-related gene expression in response to Tobacco mosaic virus pathotype P0(TMV-P0) infection. Suppression of CaLecRK-S.5 expression significantly enhanced the susceptibility to Pepper mild mottle virus pathotype P1,2,3, Xanthomonas campestris pv. vesicatoria, Phytophthora capsici, as well as TMV-P0Additionally, β-aminobutyric acid treatment and a systemic acquired resistance assay revealed that CaLecRK-S.5 is involved in priming of plant immunity. Pre-treatment with β-aminobutyric acid before viral infection restored the reduced disease resistance phenotypes shown in CaLecRK-S.5-silenced plants. Systemic acquired resistance was also abolished in CaLecRK-S.5-silenced plants. Finally, RNA sequencing analysis indicated that CaLecRK-S.5 positively regulates plant immunity at the transcriptional level. Altogether, these results suggest that CaLecRK-S.5-mediated broad-spectrum resistance is associated with the regulation of priming.
authors.affiliation: Department of Life Sciences, Korea University, Seoul 02841, Republic of Korea.
authors.forename: Joo Yong
authors.initials: JY
authors.lastname: Woo
authors.affiliation: Department of Life Sciences, Korea University, Seoul 02841, Republic of Korea.
authors.forename: Kwang Ju
authors.initials: KJ
authors.lastname: Jeong
authors.affiliation: Department of Life Sciences, Korea University, Seoul 02841, Republic of Korea.
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Life Sciences, Korea University, Seoul 02841, Republic of Korea khpaek95@korea.ac.kr.
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Paek
mesh_terms: D002212:Capsicum / Q000201:enzymology / Q000235:genetics* / Q000502:physiology / Q000821:virology; D060467:Disease Resistance / Q000235:genetics* / Q000502:physiology; D004789:Enzyme Activation; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics / Q000502:physiology*; D037102:Lectins / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D011494:Protein Kinases / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment; D014027:Tobacco Mosaic Virus
chemical_list: D037102:Lectins; D010940:Plant Proteins; D011494:Protein Kinases
keywords: Broad-spectrum resistance; CaLecRK-S.5; L-type lectin receptor kinase; RNA sequencing; SAR; priming; tobamovirus.