title: A Nano-Biosensor for the Detection of 185delAG Mutation in BRCA1 Gene, Leading to Breast Cancer.
abstract: A method was developed for the detection of 185delAG mutation in BRCA1 gene, which is responsible for 85% and 63% lifetime risks of hereditary breast and ovarian cancer in women, respectively. The protocol is based on the quenching and recovering fluorescence emission of fluorescein-based dye (FAM)-labeled DNA in the presence of graphene oxide (GO), followed by addition of cDNA or mDNA. In addition, ligase reaction between a DNA probe attached to GO and a DNA possessing FAM on 5' terminal in the presence of cDNA or mDNA was applied.
authors.affiliation: a Department of Chemistry , University of Maragheh , Maragheh , Iran.
authors.forename: Bagher
authors.initials: B
authors.lastname: Eftekhari-Sis
authors.affiliation: a Department of Chemistry , University of Maragheh , Maragheh , Iran.
authors.forename: Sajede
authors.initials: S
authors.lastname: Karaminejad
authors.affiliation: b Department of Biology , University of Maragheh , Maragheh , Iran.
authors.forename: Farrokh
authors.initials: F
authors.lastname: Karimi
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics*; D015374:Biosensing Techniques; D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics*; D003062:Codon; D004247:DNA / Q000737:chemistry; D005260:Female; D006108:Graphite / Q000737:chemistry; D006801:Humans; D036103:Nanotechnology; D010087:Oxides; D017384:Sequence Deletion; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D019313:BRCA1 Protein; D003062:Codon; D010087:Oxides; D006108:Graphite; D004247:DNA
keywords: 185delAG mutation; Breast cancer; fluorescent; graphene oxide; nano-biosensor