title: Protective effects of alpha phenyl-tert-butyl nitrone and ascorbic acid in human adipose derived mesenchymal stem cells from differently aged donors.
abstract: Adipose-derived mesenchymal stem cells (ADSCs) are multipotent stem cells that promote therapeutic effects and are frequently used in autologous applications. Little is known about how ADSCs respond to genotoxic stress and whether or not donor age affects DNA damage and repair. In this study, we used the comet assay to assess DNA damage and repair in human ADSCs derived from young (20-40 years), middle-aged (41-60 years), and older (61+ years) donors following treatment with H2O2or UV light. Tail lengths in H2O2-treated ADSCs were substantially higher than the tail lengths in UV-treated ADSCs. After 30 minutes of treatment with H2O2, ADSCs preconditioned with alpha phenyl-tert-butyl nitrone (PBN) or ascorbic acid (AA) showed a significant reduction in % tail DNA. The majority of ADSCs treated with PBN or AA displayed low olive tail movements at various timepoints. In general and indicative of DNA repair, % tail length and % tail DNA peaked at 30 minutes and then decreased to near-control levels at the 2 hour and 4 hour timepoints. Differently aged ADSCs displayed comparable levels of DNA damage in the majority of these experiments, suggesting that the age of the donor does not affect the DNA damage response in cultured ADSCs.
authors.affiliation: Department of Ophthalmology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Adiv A
authors.initials: AA
authors.lastname: Johnson
authors.affiliation: Translational Centre for Regenerative Medicine (TRM), Leipzig University, Leipzig, Germany.
authors.forename: Yahaira
authors.initials: Y
authors.lastname: Naaldijk
authors.affiliation: Department of Neurosurgery, BG Clinic Bergmannstrost Halle, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Hohaus
authors.affiliation: Department of Neurosurgery, BG Clinic Bergmannstrost Halle, Germany.
authors.forename: Hans Jörg
authors.initials: HJ
authors.lastname: Meisel
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany.
authors.forename: Ilona
authors.initials: I
authors.lastname: Krystel
authors.affiliation: Translational Centre for Regenerative Medicine (TRM), Leipzig University, Leipzig, Germany.|Loughborough University, Centre for Biological Engineering, Wolfson School, Loughborough, UK.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Stolzing
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000375:Aging; D001205:Ascorbic Acid / Q000494:pharmacology*; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008875:Middle Aged; D009589:Nitrogen Oxides / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D003497:Cyclic N-Oxides; D009589:Nitrogen Oxides; C015388:nitrones; C029217:phenyl-N-tert-butylnitrone; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid
keywords: ADSCs; DNA damage; DNA repair; aging; antioxidants; comet assay