title: Transcriptome Profiling of the Pineapple under Low Temperature to Facilitate Its Breeding for Cold Tolerance.
abstract: The pineapple (Ananas comosus) is cold sensitive. Most cultivars are injured during winter periods, especially in sub-tropical regions. There is a lack of molecular information on the pineapple's response to cold stress. In this study, high-throughput transcriptome sequencing and gene expression analysis were performed on plantlets of a cold-tolerant genotype of the pineapple cultivar 'Shenwan' before and after cold treatment. A total of 1,186 candidate cold responsive genes were identified, and their credibility was confirmed by RT-qPCR. Gene set functional enrichment analysis indicated that genes related to cell wall properties, stomatal closure and ABA and ROS signal transduction play important roles in pineapple cold tolerance. In addition, a protein association network of CORs (cold responsive genes) was predicted, which could serve as an entry point to dissect the complex cold response network. Our study found a series of candidate genes and their association network, which will be helpful to cold stress response studies and pineapple breeding for cold tolerance.
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong, P.R. China.
authors.forename: Chengjie
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong, P.R. China.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong, P.R. China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Xu
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong, P.R. China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Luan
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Mao
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong, P.R. China.
authors.forename: Junting
authors.initials: J
authors.lastname: Feng
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xie
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong, P.R. China.
authors.forename: Xue
authors.initials: X
authors.lastname: Gong
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong, P.R. China.
authors.forename: Xiaoshuang
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou, 510642, Guangdong, P.R. China.
authors.forename: Yehua
authors.initials: Y
authors.lastname: He