title: Serum and Urinary Malondialdehyde (MDA), Uric acid, and Protein as markers of perinatal asphyxia.
abstract: BACKGROUND
  Perinatal asphyxia (PA) is among the leading causes of neonatal morbidity and death in neonatal intensive care units (NICUs). The aims of this research were to determine the concentrations of malondialdehyde (MDA), urine MDA, uric acid, and protein in the cord blood of neonates with perinatal asphyxia and to determine their relationship with the severity of perinatal asphyxia.
  
  
  METHODS
  This matched case-control study was conducted from October 2012 to March 2013. All of the cases and controls were selected from the Gynecology & Obstetrics Department and the NICUs, at Qous Central Hospital in Qena, Egypt. We allocated 20 full-term neonates who had perinatal asphyxia to the case group. Also, we selected 20 healthy neonates for the control group. The subjects were matched with respect to age and gender. At birth and 48 hours later, measurements were made of MDA in cord blood and urine, and uric acid, protein, and creatinine also were measured in both groups. The data were analyzed by SPSS, version 17, using the independent-samples t-test, ANOVA, Tukey's test, and Spearman's correlation coefficient.
  
  
  RESULTS
  At birth and 48 hr later, the newborns' with PA had significantly higher levels of MDA in the cord blood, mean urinary uric acid/creatinine (UUA:Cr), protein/creatinine (UP:Cr), and MDA/creatinine ratio (UMDA:Cr) than the controls; their PA levels were correlated with the degree of hypoxic-ischemic encephalopathy (HIE). The babies who died due to PA had significantly higher levels of cord blood MDA, and they also had higher UUA:Cr, UP:Cr, and UMDA:Cr ratios than the babies who survived.
  
  
  CONCLUSIONS
  The concentration of MDA in cord blood can be used as a diagnostic marker of oxidative stress in asphyxiated neonates. The ratios of the urinary excretion rates of uric acid, protein, and MDA to creatinine increased as the severity of perinatal asphyxia and associated brain damage increased.
authors.affiliation: Professor, Department of Pediatric, Faculty of Medicine, Minia University, Minia, Egypt.
authors.forename: Sawsan Mahmoud
authors.initials: SM
authors.lastname: El Bana
authors.affiliation: Assistant Professor, Department of Pediatric, Faculty of Medicine, Minia University, Minia, Egypt.
authors.forename: Sheren Esam
authors.initials: SE
authors.lastname: Maher
authors.affiliation: Ph.D., Department of Pediatric, Qena Faculty of Medicine, South Valley University, Qena, Egypt.
authors.forename: Amani Fawzy
authors.initials: AF
authors.lastname: Gaber
authors.affiliation: Lecturer, Department of Clinical and Chemical Pathology, Qena Faculty of Medicine, South Valley University, Qena, Egypt.
authors.forename: Sanaa Shaker
authors.initials: SS
authors.lastname: Aly
keywords: MDA; Malondialdehyde; Oxidative Stress; perinatal Asphyxia