title: Recovery of ergosterol from the medicinal mushroom, Ganoderma tsugae var. Janniae, with a molecularly imprinted polymer derived from a cleavable monomer-template composite.
abstract: A semi-covalent imprinting strategy has been developed for the synthesis of molecularly-imprinted polymers specific for the fungal sterol, ergosterol, a biological precursor of vitamin D2. This imprinting approach involved a novel post-synthesis cleavable monomer-template composite, namely ergosteryl methacrylate, and resulted in the formation of an imprinted polymer that selectively and efficiently recognized ergosterol through non-covalent interactions. The derived molecularly-imprinted polymer and the corresponding non-imprinted polymer were systematically evaluated for their selectivity towards ergosterol via static and dynamic binding studies using various ergosteryl esters (e.g. ergosteryl-cinnamate, -ferulate, -coumarate, -ferulate acetate and -acetate, respectively) as competitors. Moreover, the binding capacity of the molecularly imprinted polymer for ergosterol was enhanced when the sample loading conditions involved the use of partially aqueous solvent mixtures, such as acetonitrile/water (9:1 (v/v) or 8:2 (v/v)). These attributes were exploited in a solid-phase extraction format, whereby ergosterol was obtained with excellent recoveries from an extract of the fruiting body powder of the medicinal fungus Ganoderma tsugae var. Janniae.
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia.
authors.forename: Shima N N S
authors.initials: SN
authors.lastname: Hashim
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia.
authors.forename: Lachlan J
authors.initials: LJ
authors.lastname: Schwarz
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia.
authors.forename: Basil
authors.initials: B
authors.lastname: Danylec
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia.
authors.forename: Khosse
authors.initials: K
authors.lastname: Mitri
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia.
authors.forename: Yuanzhong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia.
authors.forename: Reinhard I
authors.initials: RI
authors.lastname: Boysen
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia. Electronic address: milton.hearn@monash.edu.
authors.forename: Milton T W
authors.initials: MT
authors.lastname: Hearn
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004875:Ergosterol / Q000302:isolation & purification*; D032902:Ganoderma / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D008689:Methacrylates; D011108:Polymers; D012997:Solvents; D014867:Water; C032159:acetonitrile; D004875:Ergosterol
keywords: Ergosterol; Ergosteryl esters; Molecularly imprinted polymers; Semi-covalent molecular imprinting; Solid-phase extraction; Sterol competitors