title: Lack of TNF-α Gene Polymorphism (rs1799724) Association with Sustained Virological Response in Iranian Patients with Chronic HCV Infection.
abstract: Infection with the hepatitis C virus is a major public health concern which can lead to carcinoma and liver failure. It has been shown that single nucleotide polymorphisms can affect the level of gene activity of tumor necrosis factor (TNF) which has an important role, especially in viral infections which can lead to apaptosis of infected hepatocellular cells. We investigated the impact of three possible genotypes for rs1800629 or A/G single nucleotide polymorphism located downstream of TNFα gene promoter in groups of control (n=76) and chronic hepatitis C patients (n=89) focusing on the response to treatment among sensitive and resistant groups. Genomic DNA was extracted from 500 μl prepheral whole blood and PCR and RFLP were used to amplify the region of interest and genotyping. With statistical analyzes a p-value <0.05 was considered meaningful. There was no significant difference in distribution of the possible three genotypes among healthy individuals and patients (P=0.906, OR=1.194, CI=0.063-22.790). However, the frequency of the G allele was higher in patients whereas A allele was more common among healthy individuals (p<0.0001). Further studies with more samples appears to be necessary.
authors.affiliation: Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran E-mail : mehdi_sadat@pasteur.ac.ir.
authors.forename: Mona Sadat
authors.initials: MS
authors.lastname: Larijani
authors.forename: Narges
authors.initials: N
authors.lastname: Bahiraei
authors.forename: Mehri
authors.initials: M
authors.lastname: Nikbin
authors.forename: Nasir
authors.initials: N
authors.lastname: Mohajel
authors.forename: Leila Naghizadeh
authors.initials: LN
authors.lastname: Rad
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Baghbani
authors.forename: Maryam
authors.initials: M
authors.lastname: Mapar
authors.forename: Seyed Mehdi
authors.initials: SM
authors.lastname: Sadat
mesh_terms: D000328:Adult; D000483:Alleles; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D016174:Hepacivirus / Q000472:pathogenicity; D019698:Hepatitis C, Chronic / Q000235:genetics*; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha