title: Low Temperature Storage of Telenomus remus (Nixon) (Hymenoptera: Platygastridae) and its Factitious Host Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae).
abstract: We conducted three bioassays to evaluate the effect of low-temperature storage of eggs (host) and pupae and adults (parasitoid) on the biology and parasitism capacity of the egg parasitoid Telenomus remus (Nixon) (Hymenoptera: Platygastridae). Viable stored Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae) eggs were parasitized to the same degree or even higher than fresh eggs when stored until 14 days at 5°C or until 21 days at 10°C. In contrast, the percentage of parasitized sterilized eggs was equal to the control only when stored for 7 and 14 days. Survival of T. remus pupae declined with storage time at both studied temperatures (5 and 10°C). However, after 7 days of storage, survival of pupae was still 86.3 and 64.9% at 10 and 5°C, respectively. The number of adult male survivors remained similar until the fourth storage day at both 5 and 10°C. In contrast, female survival did not differ until day 8 at 10°C or day 6 at 5°C. Parasitism capacity of stored adults was not altered by storage compared with the control. Therefore, we conclude that the maximal storage time at 10°C is 21 days for viable C. cephalonica eggs and 7 days for T. remus pupae, while parasitoid adults should not be stored for more than 4 days at either 5 or 10°C.
authors.affiliation: Instituto Agronômico do Paraná (IAPAR), Londrina, Paraná, Brasil.
authors.forename: A P
authors.initials: AP
authors.lastname: Queiroz
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária - Embrapa Soja, Londrina, Paraná, Brasil, 86001-970. adeney.bueno@embrapa.br.
authors.forename: A F
authors.initials: AF
authors.lastname: Bueno
authors.affiliation: Univ Federal da Fronteira Sul, Laranjeiras do Sul, Paraná, Brasil.
authors.forename: A
authors.initials: A
authors.lastname: Pomari-Fernandes
authors.affiliation: Univ Estadual de Londrina, Londrina, Paraná, Brasil.
authors.forename: M L M
authors.initials: ML
authors.lastname: Grande
authors.affiliation: Univ Estadual de Londrina, Londrina, Paraná, Brasil.
authors.forename: O C
authors.initials: OC
authors.lastname: Bortolotto
authors.affiliation: Instituto Agronômico do Paraná (IAPAR), Londrina, Paraná, Brasil.
authors.forename: D M
authors.initials: DM
authors.lastname: Silva
mesh_terms: D000818:Animals; D003080:Cold Temperature; D005260:Female; D006927:Hymenoptera / Q000472:pathogenicity; D007915:Lepidoptera / Q000469:parasitology; D008297:Male; D010063:Ovum; D011679:Pupa; D013048:Specimen Handling; D013696:Temperature
keywords: Mass rearing; biological control; parasitoid shelf life