title: In vivo T-cell activation by a monoclonal αCD3ε antibody induces preterm labor and birth.
abstract: Activated/effector T cells seem to play a role in the pathological inflammation associated with preterm labor. The aim of this study was to determine whether in vivo T-cell activation by a monoclonal αCD3ε antibody induces preterm labor and birth.
  
  
  
  Pregnant B6 mice were intraperitoneally injected with a monoclonal αCD3ε antibody or its isotype control. The gestational age, the rates of preterm birth and pup mortality at birth as well as the fetal heart rate and umbilical artery pulsatility index were determined.
  
  
  
  Injection of a monoclonal αCD3ε antibody led to preterm labor/birth (αCD3ε 83 ± 16.97% [10/12] vs isotype 0% [0/8]) and increased the rate of pup mortality at birth (αCD3ε 87.30 ± 8.95% [77/85] vs isotype 4.91 ± 4.34% [3/59]). In addition, injection of a monoclonal αCD3ε antibody decreased the fetal heart rate and increased the umbilical artery pulsatility index when compared to the isotype control.
  
  
  
  In vivo T-cell activation by a monoclonal αCD3ε antibody in late gestation induces preterm labor and birth.
authors.affiliation: Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA. nardhy.gomez-lopez@wayne.edu.|Department of Obstetrics & Gynecology, Wayne State University School of Medicine, Detroit, MI, USA. nardhy.gomez-lopez@wayne.edu.|Department of Immunology & Microbiology, Wayne State University School of Medicine, Detroit, MI, USA. nardhy.gomez-lopez@wayne.edu.
authors.forename: Nardhy
authors.initials: N
authors.lastname: Gomez-Lopez
authors.affiliation: Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA. romeror@mail.nih.gov.|Department of Obstetrics & Gynecology, University of Michigan, Ann Arbor, MI, USA. romeror@mail.nih.gov.|Department of Epidemiology & Biostatistics, Michigan State University, East Lansing, MI, USA. romeror@mail.nih.gov.|Center for Molecular Obstetrics & Genetics, Wayne State University, Detroit, MI, USA. romeror@mail.nih.gov.
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero
authors.affiliation: Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA.|Department of Obstetrics & Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.|Department of Molecular Biomedicine, CINVESTAV, Mexico City, Mexico.
authors.forename: Marcia
authors.initials: M
authors.lastname: Arenas-Hernandez
authors.affiliation: Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA.|Department of Obstetrics & Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
authors.forename: Hyunyoung
authors.initials: H
authors.lastname: Ahn
authors.affiliation: Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA.|Department of Obstetrics & Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Panaitescu
authors.affiliation: Unit of Vinculation, Faculty of Medicine, Universidad Nacional Autónoma de México en el Instituto Nacional de Medicina Genómica, Mexico City, Mexico.
authors.forename: Felipe
authors.initials: F
authors.lastname: Vadillo-Ortega
authors.affiliation: Department of Molecular Biomedicine, CINVESTAV, Mexico City, Mexico.
authors.forename: Carmen
authors.initials: C
authors.lastname: Sanchez-Torres
authors.affiliation: Department of Obstetrics & Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
authors.forename: Katherine S
authors.initials: KS
authors.lastname: Salisbury
authors.affiliation: Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA.|Department of Obstetrics & Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
authors.forename: Sonia S
authors.initials: SS
authors.lastname: Hassan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D017252:CD3 Complex / Q000276:immunology; D005260:Female; D006801:Humans; D007274:Injections, Intraperitoneal; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D007752:Obstetric Labor, Premature / Q000276:immunology*; D011247:Pregnancy; D047928:Premature Birth / Q000276:immunology*; D050497:Stillbirth; D013601:T-Lymphocytes / Q000276:immunology*; D014469:Umbilical Arteries / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; C485468:Cd3e protein, mouse
keywords: T cells; adaptive immunity; cytokines; maternal-fetal rejection; mouse; parturition; pregnancy