title: Nontargeted LC-MSnProfiling of Compounds in Ileal Fluids That Decrease after Raspberry Intake Identifies Consistent Alterations in Bile Acid Composition.
abstract: Ileostomy studies provide a unique insight into the digestion of foods, allowing identification of physiologically relevant dietary phytochemicals and their metabolites that are important to gut health. We previously reported an increase of components, including novel triterpenoids, in ileal fluids of 11 ileostomates following consumption of raspberries using nontargeted LC-MSntechniques in combination with data deconvolution software. The current study focused on components that consistently decreased postsupplementation. After data deconvolution, 32 components were identified that met exclusion parameters of m/z signals and which decreased significantly in ileal fluids from eight of 11 participants post-raspberry supplementation. Two-thirds of these components were identified putatively from their MS properties. Consistent decreases were observed in components that possibly reflected "washing out" of presupplementation intake of common foods/drinks including (poly)phenol metabolites. Metabolites associated with fat metabolism such as hydroxylated fatty acids and cholate-type bile acids were specifically reduced. However, more directed re-examination of the data revealed that although some cholates were consistently reduced, the more polar glyco- and tauro-linked bile acid derivatives increased consistently, by as much as 100-fold over presupplementation levels. The possible reasons for these substantial alterations in bile acid composition in ileal fluids in response to raspberry intake are discussed.
authors.affiliation: Environmental and Biochemical Sciences Group, The James Hutton Institute , Invergowrie, Dundee, DD2 5DA, Scotland.
authors.forename: Gordon J
authors.initials: GJ
authors.lastname: McDougall
authors.affiliation: Environmental and Biochemical Sciences Group, The James Hutton Institute , Invergowrie, Dundee, DD2 5DA, Scotland.
authors.forename: J William
authors.initials: JW
authors.lastname: Allwood
authors.affiliation: Postharvest, Technology and Agrifood Industry Area, IFAPA , Córdoba, Spain.
authors.forename: Gema
authors.initials: G
authors.lastname: Pereira-Caro
authors.affiliation: Centre for Molecular Biosciences, University of Ulster , Coleraine, BT52 1SA, Northern Ireland.
authors.forename: Emma M
authors.initials: EM
authors.lastname: Brown
authors.affiliation: Altnagelvin Area Hospital, Western Health and Social Care Trust , Londonderry, BT47 6SB, Northern Ireland.
authors.forename: Nigel
authors.initials: N
authors.lastname: Ternan
authors.affiliation: Environmental and Biochemical Sciences Group, The James Hutton Institute , Invergowrie, Dundee, DD2 5DA, Scotland.
authors.forename: Susan
authors.initials: S
authors.lastname: Verrall
authors.affiliation: Environmental and Biochemical Sciences Group, The James Hutton Institute , Invergowrie, Dundee, DD2 5DA, Scotland.|School of Life Sciences, Heriot-Watt University , Edinburgh, EH14 4AS, Scotland.
authors.forename: Derek
authors.initials: D
authors.lastname: Stewart
authors.affiliation: Altnagelvin Area Hospital, Western Health and Social Care Trust , Londonderry, BT47 6SB, Northern Ireland.
authors.forename: Roger
authors.initials: R
authors.lastname: Lawther
authors.affiliation: Altnagelvin Area Hospital, Western Health and Social Care Trust , Londonderry, BT47 6SB, Northern Ireland.
authors.forename: Gloria
authors.initials: G
authors.lastname: O'Connor
authors.affiliation: Department of Food and Nutritional Sciences, University of Reading , Reading, RG6 6AP, England.
authors.forename: Ian
authors.initials: I
authors.lastname: Rowland
authors.affiliation: Department of Nutrition, University of California , Davis, California 95616, United States.
authors.forename: Alan
authors.initials: A
authors.lastname: Crozier
authors.affiliation: Centre for Molecular Biosciences, University of Ulster , Coleraine, BT52 1SA, Northern Ireland.
authors.forename: Chris I R
authors.initials: CI
authors.lastname: Gill
mesh_terms: D001647:Bile Acids and Salts / Q000737:chemistry / Q000378:metabolism*; D002853:Chromatography, Liquid; D019587:Dietary Supplements; D006801:Humans; D007082:Ileum / Q000378:metabolism*; D008297:Male; D015394:Molecular Structure; D065856:Rubus / Q000737:chemistry*
chemical_list: D001647:Bile Acids and Salts