title: An ultrasensitive electrochemiluminescence biosensor for the detection of concanavalin A based on Au nanoparticles-thiosemicarbazide functionalized PtNi nanocubes as signal enhancer.
abstract: A sandwich-configuration electrochemiluminescence (ECL) biosensor was constructed for detecting concanavalin A (ConA) based on peroxydisulfate/oxygen (S2O82-/O2) system. In this work, the gold nanoflower modified Zn-doped SnO2was used as a substrate to adsorb recognition element horseradish peroxidase (HRP) for binding ConA. Then, Au nanoparticles-thiosemicarbazide functionalized PtNi nanocubes (AuNPs-TSC-PtNi NCs), as a novel ECL signal tracer, were incubated onto the electrode through a specific carbohydrate-ConA interaction, thus achieving a sandwiched structure. The integration of amplifying effect of both TSC and PtNi NCs on the ECL of S2O82-/O2system endowed the biosensor a high sensitivity. The linear range for ConA detection was from 0.0010ng/mL to 10ng/mL with a detection limit of 0.0002ng/mL (S/N=3).
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Endocrinology, 9th People's Hospital of Chongqing, Chongqing 400700, China.
authors.forename: Xingrong
authors.initials: X
authors.lastname: Tan
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ou
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: cshong@swu.edu.cn.
authors.forename: Shihong
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: shapingw@swu.edu.cn.
authors.forename: Shaping
authors.initials: S
authors.lastname: Wei
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D003208:Concanavalin A / Q000032:analysis / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009532:Nickel / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D012663:Semicarbazides / Q000737:chemistry*
chemical_list: D012663:Semicarbazides; D003208:Concanavalin A; D010984:Platinum; C005151:thiosemicarbazide; D006046:Gold; D009532:Nickel; D006735:Horseradish Peroxidase
keywords: ConA; Electrochemiluminescence; Peroxydisulfate/oxygen system; PtNi nanocube