title: Cellular Activity of New Small Molecule Protein Arginine Deiminase 3 (PAD3) Inhibitors.
abstract: The protein arginine deiminases (PADs) catalyze the post-translational deimination of arginine side chains. Multiple PAD isozymes have been characterized, and abnormal PAD activity has been associated with several human disease states. PAD3 has been characterized as a modulator of cell growth via apoptosis inducing factor and has been implicated in the neurodegenerative response to spinal cord injury. Here, we describe the design, synthesis, and evaluation of conformationally constrained versions of the potent and selective PAD3 inhibitor 2. The cell activity of representative inhibitors in this series was also demonstrated for the first time by rescue of thapsigargin-induced cell death in PAD3-expressing HEK293T cells.
authors.affiliation: Department of Chemistry, Yale University , New Haven, Connecticut 06520, United States.
authors.forename: Haya
authors.initials: H
authors.lastname: Jamali
authors.affiliation: Department of Chemistry, Yale University , New Haven, Connecticut 06520, United States.
authors.forename: Hasan A
authors.initials: HA
authors.lastname: Khan
authors.affiliation: Department of Chemistry, Yale University , New Haven, Connecticut 06520, United States.
authors.forename: Caroline C
authors.initials: CC
authors.lastname: Tjin
authors.affiliation: Department of Chemistry, Yale University , New Haven, Connecticut 06520, United States.
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Ellman
keywords: Protein arginine deiminases; apoptosis inducing factor; small molecule inhibitor; spinal cord injury