title: Obesity Exacerbates Sepsis-Induced Oxidative Damage in Organs.
abstract: Sepsis progression is linked to the imbalance between reactive oxygen species and antioxidant enzymes. Sepsis affects multiple organs, but when associated with a chronic inflammatory disease, such as obesity, it may be exacerbated. We hypothesized that obesity could aggravate the oxidative damage to peripheral organs of rats submitted to an animal model of sepsis. Male Wistar rats aged 8 weeks received hypercaloric nutrition for 2 months to induce obesity. Sepsis was induced by cecal ligation and puncture (CLP) procedure, and sham-operated rats were considered as control group. The experimental groups were divided into sham + eutrophic, sham + obese, CLP + eutrophic, and CLP + obese. Twelve and 24 h after surgery, oxidative damage to lipids and proteins and superoxide dismutase (SOD) and catalase (CAT) activities were evaluated in the liver, lung, kidney, and heart. The data indicate that obese rats subjected to sepsis present oxidative stress mainly in the lung and liver. This alteration reflected an oxidative damage to lipids and proteins and an imbalance of SOD and CAT levels, especially 24 h after sepsis. It follows that obesity due to its pro-inflammatory phenotype can aggravate sepsis-induced damage in peripheral organs.
authors.affiliation: Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Postgraduate Program in Health Sciences, University of South Santa Catarina, Avenida José Acácio Moreira, 787, Tubarao, SC, 88704-900, Brazil. fabricia.petronilho@unisul.br.
authors.forename: Fabricia
authors.initials: F
authors.lastname: Petronilho
authors.affiliation: Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Postgraduate Program in Health Sciences, University of South Santa Catarina, Avenida José Acácio Moreira, 787, Tubarao, SC, 88704-900, Brazil.
authors.forename: Amanda Della
authors.initials: AD
authors.lastname: Giustina
authors.affiliation: Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Postgraduate Program in Health Sciences, University of South Santa Catarina, Avenida José Acácio Moreira, 787, Tubarao, SC, 88704-900, Brazil.
authors.forename: Diego Zapelini
authors.initials: DZ
authors.lastname: Nascimento
authors.affiliation: Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Postgraduate Program in Health Sciences, University of South Santa Catarina, Avenida José Acácio Moreira, 787, Tubarao, SC, 88704-900, Brazil.
authors.forename: Graciela Freitas
authors.initials: GF
authors.lastname: Zarbato
authors.affiliation: Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Postgraduate Program in Health Sciences, University of South Santa Catarina, Avenida José Acácio Moreira, 787, Tubarao, SC, 88704-900, Brazil.
authors.forename: Andriele Aparecida
authors.initials: AA
authors.lastname: Vieira
authors.affiliation: Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Postgraduate Program in Health Sciences, University of South Santa Catarina, Avenida José Acácio Moreira, 787, Tubarao, SC, 88704-900, Brazil.
authors.forename: Drielly
authors.initials: D
authors.lastname: Florentino
authors.affiliation: Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Postgraduate Program in Health Sciences, University of South Santa Catarina, Avenida José Acácio Moreira, 787, Tubarao, SC, 88704-900, Brazil.
authors.forename: Lucinéia Gainski
authors.initials: LG
authors.lastname: Danielski
authors.affiliation: Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Postgraduate Program in Health Sciences, University of South Santa Catarina, Avenida José Acácio Moreira, 787, Tubarao, SC, 88704-900, Brazil.
authors.forename: Mariana Pereira
authors.initials: MP
authors.lastname: Goldim
authors.affiliation: Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Postgraduate Program in Health Sciences, University of South Santa Catarina, Avenida José Acácio Moreira, 787, Tubarao, SC, 88704-900, Brazil.
authors.forename: Gislaine Tezza
authors.initials: GT
authors.lastname: Rezin
authors.affiliation: Center for Experimental Models in Psychiatry, Department of Psychiatry and Behavioral Sciences, The University of Texas Medical School at Houston, Houston, TX, USA.|Laboratory of Experimental Microbiology, Graduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, SC, Brazil.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Barichello
mesh_terms: D000818:Animals; D002374:Catalase; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000293:injuries / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D055370:Lung Injury / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009765:Obesity / Q000150:complications*; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000150:complications*; D013482:Superoxide Dismutase
chemical_list: D002374:Catalase; D013482:Superoxide Dismutase
keywords: inflammation; obesity; organs; oxidative stress; sepsis