title: Development and evaluation of a multiplex real-time PCR for the detection of IMP, VIM, and OXA-23 carbapenemase gene families on the BD MAX open system.
abstract: A multiplex real-time polymerase chain reaction (PCR) assay was developed for the detection of clinically prevalent IMP, VIM, and OXA-23 gene families. The assay was designed to work on the BD MAX open platform which is a fully automated system for all PCR processes including sample extraction to PCR resulting. A total of 107 well-characterized carbapenem resistant Enterobacteriaceae were evaluated and the results were 100% concordant with the reference test isolates. This assay will serve to complement PCR screens that detect the major carbapenemase families of NDM, KPC, and OXA-48-like.
authors.affiliation: National University Hospital, Department of Laboratory Medicine, Division of Microbiology, Singapore, 119074, Republic of Singapore. Electronic address: Jeanette_Teo@nuhs.edu.sg.
authors.forename: Jeanette W P
authors.initials: JW
authors.lastname: Teo
authors.affiliation: National Public Health Laboratory, Ministry of Health, 3 Biopolis Drive, Synapse #05-14/16, Singapore, 138623, Republic of Singapore.
authors.forename: My-Van
authors.initials: MV
authors.lastname: La
authors.affiliation: National University Hospital, Department of Laboratory Medicine, Division of Microbiology, Singapore, 119074, Republic of Singapore; National Public Health Laboratory, Ministry of Health, 3 Biopolis Drive, Synapse #05-14/16, Singapore, 138623, Republic of Singapore.
authors.forename: Raymond T P
authors.initials: RT
authors.lastname: Lin
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Carbapenem resistance; Enterobacteriaceae; Real-time PCR