title: Amyloid Plaques Show Binding Capacity of Exogenous Injected Amyloid-β.
abstract: Amyloid plaques, although inducing damage to the immediately surrounding neuropil, have been proposed to provide a relatively innocuous way to deposit toxic soluble amyloid-β (Aβ) species. Here we address this hypothesis by exploring spread and absorption of fluorescent Aβ to pre-existing amyloid plaques after local application in wild-type mice versus APP/PS1 transgenic mice with amyloid plaques. Local intracortical or intracerebroventricular injection of fluorescently-labeled Aβ in APP/PS1 mice with a high plaque density resulted in preferential accumulation of the peptide in amyloid plaques in both conventional postmortem histology and in live imaging using two-photon microscopy. These findings support the contention that amyloid plaques may act as buffers to protect neurons from the toxic effects of momentary high concentrations of soluble Aβ oligomers.
authors.affiliation: A.I. Virtanen Institute, University of Eastern Finland, Kuopio, Finland.
authors.forename: Irina
authors.initials: I
authors.lastname: Gureviciene
authors.affiliation: A.I. Virtanen Institute, University of Eastern Finland, Kuopio, Finland.
authors.forename: Kestutis
authors.initials: K
authors.lastname: Gurevicius
authors.affiliation: Neuroscience Center, University of Helsinki, Helsinki, Finland.|Institute of Theoretical & Experimental Biophysics, RAS, Pushchino, Russia.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Mugantseva
authors.affiliation: Neuroscience Center, University of Helsinki, Helsinki, Finland.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Kislin
authors.affiliation: Neuroscience Center, University of Helsinki, Helsinki, Finland.|Neurotar Ltd, Helsinki, Finland, http://www.neurotar.com.
authors.forename: Leonard
authors.initials: L
authors.lastname: Khiroug
authors.affiliation: A.I. Virtanen Institute, University of Eastern Finland, Kuopio, Finland.
authors.forename: Heikki
authors.initials: H
authors.lastname: Tanila
mesh_terms: D016229:Amyloid beta-Peptides / Q000008:administration & dosage / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism; D000818:Animals; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D010446:Peptide Fragments / Q000008:administration & dosage / Q000378:metabolism*; D058225:Plaque, Amyloid / Q000378:metabolism* / Q000473:pathology; D053764:Presenilin-1 / Q000235:genetics / Q000378:metabolism
chemical_list: C545487:APP protein, human; D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D005456:Fluorescent Dyes; C508853:PSEN1 protein, human; D010446:Peptide Fragments; D053764:Presenilin-1; C075222:amyloid beta-protein (1-42)
keywords: Alzheimer’s disease; amyloid-β; in vivo imaging; multiphoton; transgenic