title: CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical Valve.
abstract: The hyphenation of capillary electrophoresis and electrospray ionization-mass spectrometry is a powerful tool for peptide and protein analysis. It provides high separation power in combination with sensitive and selective detection and the possibility of analyte identification. Unfortunately, many proven capillary electrophoresis methods are not compatible with electrospray-mass spectrometry since several compounds of best separating background electrolytes are interfering in the electrospray ionization. Here, we describe a two-dimensional capillary electrophoresis system using the second dimension as a cleanup stage in order to remove interfering compounds to enable electrospray-mass spectrometry coupling.
authors.affiliation: Department of Chemistry, Aalen University, Beethovenstraβe 1, Aalen, D-73430, Germany.
authors.forename: Felix J
authors.initials: FJ
authors.lastname: Kohl
authors.affiliation: Department of Chemistry, Aalen University, Beethovenstraβe 1, Aalen, D-73430, Germany. Christian.neusuess@htw-aalen.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Neusüß
mesh_terms: D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004573:Electrolytes; D010455:Peptides
keywords: 2D; Capillary electrophoresis; Electrospray ionization; Heart-cut; Mass spectrometry; Peptides; Proteins