title: Aluminum hydroxide nanoparticles show strong activity to stimulate Th-1 immune response against tuberculosis.
abstract: Many materials such as aluminum hydroxide have been tried as adjuvants to compensate low inherent immunogenicity of subunit vaccines. The aim of this study was to evaluate the specific immune response following the administration of aluminum hydroxide nanoparticles with EsxV antigen. The physiochemical properties of the nanoparticle were characterized in vitro. After subcutaneous immunization, cytokine secretion patterns including IFN-gama,IL-4, and TGF-beta levels were measured by indirect enzyme linked immunosorbent assay (ELISA). Aluminum hydroxide-NPs were demonstrated excellent effects to raise of IFN-γ secretion in compare to EsxV alone. Administration of aluminum hydroxide nanoparticles stimulates strong cellular immune response and could be considered as appropriate adjuvant against TB infection.
authors.affiliation: a Molecular Biology Research Center, Baqiyatallah University of Medical Sciences , Tehran , Iran.
authors.forename: Yousef
authors.initials: Y
authors.lastname: Amini
authors.affiliation: b Esfarayen Faculty of Medical Sciences , Esfarayen , Iran.
authors.forename: Bagher
authors.initials: B
authors.lastname: Moradi
authors.affiliation: a Molecular Biology Research Center, Baqiyatallah University of Medical Sciences , Tehran , Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Fasihi-Ramandi
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D000327:Adsorption; D000536:Aluminum Hydroxide / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D053758:Nanoparticles; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D014376:Tuberculosis / Q000276:immunology* / Q000628:therapy
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D000536:Aluminum Hydroxide
keywords: EsxV; Tuberculosis; aluminum hydroxide; immune response; nanoparticles