title: Utility of a Dengue-Derived Monoclonal Antibody to Enhance Zika Infection In Vitro.
abstract: BACKGROUND
  Zika virus (ZIKV) has emerged in dengue (DENV) endemic areas, where these two related flaviviruses continue to co-circulate. DENV is a complex of four serotypes and infections can progress to severe disease. It is thought that this is mediated by antibody dependent enhancement (ADE) whereby antibodies from a primary DENV infection are incapable of neutralizing heterologous DENV infections with another serotype. ADE has been demonstrated among other members of the Flavivirus group.
  
  
  METHODS
  We utilize an in vitro ADE assay developed for DENV to determine whether ZIKV is enhanced by a commonly available DENV serotype 2-derived monoclonal antibody (4G2).
  
  
  RESULTS
  We show that ZIKV infection in vitro is enhanced in the presence of the 4G2 mAb.
  
  
  CONCLUSIONS
  Our results demonstrate that ADE between ZIKV and DENV is possible and that the 4G2 antibody is a useful tool for the effects of pre-existing anti-DENV antibodies during ZIKV infections.
authors.affiliation: Pathobiological Sciences, Louisiana State University, Baton Rouge, Louisiana, USA.
authors.forename: Anu Susan
authors.initials: AS
authors.lastname: Charles
authors.affiliation: Pathobiological Sciences, Louisiana State University, Baton Rouge, Louisiana, USA.
authors.forename: Rebecca C
authors.initials: RC
authors.lastname: Christofferson
keywords: Zika; antibody; dengue; enhancement; zika viru