title: Suppression of Myostatin Stimulates Regenerative Potential of Injured Antigravitational Soleus Muscle in Mice under Unloading Condition.
abstract: Effects of myostatin (MSTN)-suppression on the regeneration of injured skeletal muscle under unloading condition were investigated by using transgenic mice expressing a dominant-negative form of MSTN (MSTN-DN). Both MSTN-DN and wild-type (WT) mice were subjected to continuous hindlimb suspension (HS) for 6 weeks. Cardiotoxin (CTX) was injected into left soleus muscle under anesthesia 2 weeks after the initiation of HS. Then, the soleus muscles were excised following 6-week HS (4 weeks after CTX-injection). CTX-injection caused to reduce the soleus fiber cross-sectional area (CSA) in WT mice under both unloading and weight-bearing conditions, but not in MSTN-DN mice. Under unloading condition, CTX-injected muscle weight and fiber CSA in MSTN-DN mice were significantly higher than those in WT mice. CTX-injected muscle had many damaged and regenerating fibers having central nuclei in both WT and MSTN-DN mice. Significant increase in the population of Pax7-positive nuclei in CTX-injected muscle was observed in MSTN-DN mice, but not in WT mice. Evidences indicate that the suppression of MSTN cause to increase the regenerative potential of injured soleus muscle via the increase in the population of muscle satellite cells regardless of unloading conditions.
authors.affiliation: Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi, Aichi, Japan;
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Ohno
authors.affiliation: Department of Orthopaedic Surgery, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan;
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Matsuba
authors.affiliation: Department of Regenerative Medicine, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan;
authors.forename: Naohiro
authors.initials: N
authors.lastname: Hashimoto
authors.affiliation: Faculty of Education, Yamaguchi University, Yamaguchi City, Yamaguchi, Japan;
authors.forename: Takao
authors.initials: T
authors.lastname: Sugiura
authors.affiliation: Graduate School of Health and Sports Science, Doshisha University, Kyotanabe, Kyoto, Japan;
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ohira
authors.affiliation: Hirosaki Gakuin University, Hirosaki, Aomori, Japan;
authors.forename: Toshitada
authors.initials: T
authors.lastname: Yoshioka
authors.affiliation: Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi, Aichi, Japan;; Department of Physiology, Graduate School of Health Sciences, Toyohashi SOZO University, Toyohashi, Aichi, Japan.
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Goto
mesh_terms: D000818:Animals; D054715:Cardiotoxins / Q000008:administration & dosage; D006614:Hindlimb / Q000187:drug effects / Q000254:growth & development* / Q000293:injuries / Q000503:physiopathology; D006801:Humans; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000473:pathology; D018482:Muscle, Skeletal / Q000187:drug effects / Q000254:growth & development* / Q000293:injuries / Q000503:physiopathology; D055435:Myostatin / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism / Q000473:pathology; D016474:Weight-Bearing
chemical_list: D054715:Cardiotoxins; D055435:Myostatin
keywords: muscle regeneration; muscle satellite cell.; myostatin; skeletal muscle; unloading