title: The Impact of Treatment on the Serum Concentration of Interleukin-1 Beta in Pulmonary Tuberculosis.
abstract: Tuberculosis (TB) is a major public health problem in the world. Further elucidation of the pathogenesis and identification of suitable biomarkers of the disease have remained research priorities. Interleukin-1 beta (IL-1 β) signaling is known to be high in active tuberculosis. In this study, we followed up a cohort of adult sputum-positive tuberculosis patients and investigated the impact of anti-tuberculosis treatment on the serum concentrations of IL-1 β. The protocol was approved by the Ethics Committee of University of Nigeria Teaching Hospital Enugu. Each participant gave informed consent. Serum concentration of Interleukin-1 was measured before treatment, after 2 months of treatment, and after 6 months of treatment by Enzyme linked immunosorbent assay method. Forty-two tuberculosis patients and 31 healthy volunteers completed the study. The patients had good clinical response to treatment. The mean serum concentration of Interleukin-1 beta (IL-1 β) for the patients before treatment was very high (30.20 ± 2.0 pg/mL) compared with those of healthy controls (13.30 ± 1.30). As treatment progressed there was remarkable, progressive and statistically significant (P < 0.05) reduction in the mean IL-1 β serum concentration of the patients: 21.80 ± 1.1 pg/ml after 2 months and 16.96 ± 1.3 pg/mL after 6 months treatment. At the completion of treatment, the mean serum concentration of IL-1 β of the patients was comparable but slightly higher than those of the healthy controls. Serum concentration of Interleukin-1 beta is thus considered a potential host biomarker for active tuberculosis in adult humans.
authors.affiliation: Department of Pharmacology and Therapeutics, College of Medicine, University of Nigeria Nsukka, Enugu Campus, Nigeria.
authors.forename: Chikere A
authors.initials: CA
authors.lastname: Anusiem
authors.forename: Paul O
authors.initials: PO
authors.lastname: Okonkwo
mesh_terms: D000328:Adult; D000995:Antitubercular Agents / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006784:Hospitals, Teaching; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009549:Nigeria; D013183:Sputum / Q000382:microbiology; D016896:Treatment Outcome; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000188:drug therapy*; D055815:Young Adult
chemical_list: D000995:Antitubercular Agents; D015415:Biomarkers; D053583:Interleukin-1beta