title: Bordetella bronchisepticaantigen enhances the production ofMycoplasma hyopneumoniaeantigen-specific immunoglobulin G in mice.
abstract: We previously demonstrated thatBordetella(B.)bronchisepticaantigen (Ag) showed high immunostimulatory effects on mouse bone marrow cells (BMs) whileMycoplasma(M.)hyopneumoniaeAg showed low effects. The focus of this study was to determine ifB. bronchisepticaAg can enhance theM. hyopneumoniaeAg-specific immune response and whether the host's immune system can recognize both Ags. MTT assay results revealed that each or both Ags did not significantly change BM metabolic activity. Flow cytometry analysis using carboxyfluorescein succinimidyl ester showed thatB. bronchisepticaAg can promote the division of BMs. In cytokine and nitric oxide (NO) assays,B. bronchisepticaAg boosted production of tumor necrosis factor-alpha inM. hyopneumoniaeAg-treated BMs, and combined treatment with both Ags elevated the level of NO in BMs compared to that from treatment ofM. hyopneumoniaeAg alone. Immunoglobulin (Ig)G enzyme-linked immunosorbent assay using the sera of Ag-injected mice clearly indicated thatB. bronchisepticaAg can increase the production ofM. hyopneumoniaeAg-specific IgG. This study provided information valuable in the development ofM. hyopneumoniaevaccines and showed thatB. bronchisepticaAg can be used both as a vaccine adjuvant and as a vaccine Ag.
authors.affiliation: College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea.
authors.forename: Seol-Hwa
authors.initials: SH
authors.lastname: Yim
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea.
authors.forename: Tae-Wook
authors.initials: TW
authors.lastname: Hahn
authors.affiliation: College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea.
authors.forename: Hong-Gu
authors.initials: HG
authors.lastname: Joo
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000917:Antibody Formation / Q000187:drug effects; D000942:Antigens, Bacterial / Q000276:immunology* / Q000494:pharmacology; D016950:Bordetella bronchiseptica / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D009569:Nitric Oxide / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D016207:Cytokines; D007074:Immunoglobulin G; D009569:Nitric Oxide
keywords: Bordetella bronchiseptica; Mycoplasma hyopneumoniae; antigen-specific immune response; bone marrow cells; vaccine adjuvant