title: Differential deposition of H2A.Z in combination with histone modifications within related genes in Oryza sativa callus and seedling.
abstract: As a histone variant, H2A.Z is highly conserved among species and plays a significant role in diverse cellular processes. Here, we generated genome-wide maps of H2A.Z in Oryza sativa (rice) callus and seedling by combining chromatin immunoprecipitation using H2A.Z antibody and high-throughput sequencing. We found a significantly high peak and a small peak of H2A.Z distributed at the 5' and 3' ends of highly expressed genes, respectively. H2A.Z was also associated with inactive genes in both tissues. H3 lysine 4 trimethylation was associated with H2A.Z deposition at the 5' end of expressed genes, and H3 lysine 27 trimethylation peaks were partially associated with H2A.Z. In summary, our study provides global analysis data for the distribution of H2A.Z in the rice genome. Our results demonstrate that the differential deposition of H2A.Z might play important roles in gene transcription during rice development.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Chunchao
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yi
authors.affiliation: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP), Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Su
mesh_terms: D047369:Chromatin Immunoprecipitation; D002874:Chromosome Mapping; D019175:DNA Methylation; D018506:Gene Expression Regulation, Plant; D042421:Histone Code; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D036226:Seedlings / Q000235:genetics; D024363:Transcription Initiation Site
chemical_list: D006657:Histones; D010940:Plant Proteins; D008239:Lysine
keywords: 
  Oryza sativa
  ; ChIP-seq; H2A.Z; distribution; gene expression