title: Therapeutic effect of monophosphoryl lipid A administration on Paracoccidioides brasiliensis-infected mice.
abstract: The lack of antifungals with low toxicity and short-term therapy for patients with paracoccidioidomycosis (PCM) led us to evaluate adjuvants in immunotherapeutic intervention. We have previously shown complete Freund's adjuvant (CFA) to be therapeutic on experimental PCM. Owing to CFA toxicity, here we tested adjuvants approved for clinical use or in preclinical phase in experimental mouse PCM. Of all, only monophosporyl lipid A (MPLA) demonstrates a beneficial effect, by reducing the fungal burden and increasing the concentrations of IFN-γ and TNF-α, which are immunoprotective in PCM. These results suggest that MPLA might improve intervention in PCM.
authors.affiliation: Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Taise Natali
authors.initials: TN
authors.lastname: Landgraf
authors.affiliation: Department of Cellular and Molecular Biology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Fabrício Freitas
authors.initials: FF
authors.lastname: Fernandes
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, Campinas, SP.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Peron
authors.affiliation: Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Ademilson
authors.initials: A
authors.lastname: Panunto-Castelo
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007155:Immunologic Factors / Q000008:administration & dosage*; D008050:Lipid A / Q000008:administration & dosage / Q000031:analogs & derivatives*; D008297:Male; D008807:Mice, Inbred BALB C; D010229:Paracoccidioidomycosis / Q000188:drug therapy*; D045506:Therapeutic Uses; D016896:Treatment Outcome
chemical_list: D007155:Immunologic Factors; D008050:Lipid A; D045506:Therapeutic Uses; C048436:monophosphoryl lipid A
keywords: Paracoccidioides brasiliensis; adjuvants; immunotherapy; monophosphoryl lipid A; paracoccidioidomycosis