title: The healing effect of Pistacia lentiscus fruit oil on laser burn.
abstract: BACKGROUND
  Since antiquity, Pistacia lentiscus L. (Anacardiaceae) fruit oil (PLFO) has been used as a remedy for primary health care such as burn treatment.
  
  
  OBJECTIVE
  This study assesses the healing effect of PLFO on CO2laser fractional burn in a rat model.
  
  
  METHODS
  The study was carried out on 18 adult male Wistar rats. A second-degree laser burn (wound area = 2.2 cm2) was inflicted in the dorsal region by the application of CO2fractional laser within the following parameters; Energy level: 25 MJ and Depth level: 4. After applying laser, the rats were divided into three groups: the first was treated with saline solution, the second with a reference cream 'CYTOL BASIC®' (0.13 μg/mm2) and the third with PLFO (0.52 μL/mm2). All treatments were topically administered for eight days. The healing effect was assessed using macroscopic, histological and biochemical parameters.
  
  
  RESULTS
  After eight days, the higher percentage of wound healing contraction was observed among the PLFO-treated group (100%) followed by the 'CYTOL BASIC®' treated group (61.36%) and untreated group (32.27%). During the treatment, the PLFO-treated group showed less erythema, less crusting/scabbing, higher general wound appearance scores and a high content of collagen (220.67 ± 7.48 mg/g of tissue) than the other groups.
  
  
  CONCLUSIONS
  The current study has shown, for the first time, the healing effect of PLFO on CO2laser fractional burn. Their wound healing effect could be attributed to their antimicrobial, anti-inflammatory and antioxidant effects.
authors.affiliation: a Laboratory of Histology, Embryology and Reproductive Biology, Faculty of Medicine of Sfax , University of Sfax , Sfax, Tunisia.
authors.forename: Sameh Ben
authors.initials: SB
authors.lastname: Khedir
authors.affiliation: b Laboratory of Pharmacology, Faculty of Medicine of Sfax , University of Sfax , Sfax, Tunisia.
authors.forename: Sana
authors.initials: S
authors.lastname: Bardaa
authors.affiliation: c Private Center of Plastic Surgery , Sfax , Tunisia.
authors.forename: Naourez
authors.initials: N
authors.lastname: Chabchoub
authors.affiliation: b Laboratory of Pharmacology, Faculty of Medicine of Sfax , University of Sfax , Sfax, Tunisia.
authors.forename: Dorsaf
authors.initials: D
authors.lastname: Moalla
authors.affiliation: b Laboratory of Pharmacology, Faculty of Medicine of Sfax , University of Sfax , Sfax, Tunisia.
authors.forename: Zouheir
authors.initials: Z
authors.lastname: Sahnoun
authors.affiliation: a Laboratory of Histology, Embryology and Reproductive Biology, Faculty of Medicine of Sfax , University of Sfax , Sfax, Tunisia.
authors.forename: Tarek
authors.initials: T
authors.lastname: Rebai
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D002056:Burns / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D003094:Collagen / Q000378:metabolism; D003879:Dermatologic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004195:Disease Models, Animal; D005638:Fruit / Q000737:chemistry*; D006909:Hydroxyproline / Q000378:metabolism; D054020:Lasers, Gas; D008297:Male; D008517:Phytotherapy; D027927:Pistacia / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D003879:Dermatologic Agents; D010936:Plant Extracts; D010938:Plant Oils; D003094:Collagen; D006909:Hydroxyproline
keywords: Wound contraction; collagen; fatty acids; sterol; tocopherol