title: Pathway Design, Engineering, and Optimization.
abstract: The microbial metabolic versatility found in nature has inspired scientists to create microorganisms capable of producing value-added compounds. Many endeavors have been made to transfer and/or combine pathways, existing or even engineered enzymes with new function to tractable microorganisms to generate new metabolic routes for drug, biofuel, and specialty chemical production. However, the success of these pathways can be impeded by different complications from an inherent failure of the pathway to cell perturbations. Pursuing ways to overcome these shortcomings, a wide variety of strategies have been developed. This chapter will review the computational algorithms and experimental tools used to design efficient metabolic routes, and construct and optimize biochemical pathways to produce chemicals of high interest.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
authors.forename: Eva
authors.initials: E
authors.lastname: Garcia-Ruiz
authors.affiliation: Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
authors.forename: Mohammad
authors.initials: M
authors.lastname: HamediRad
authors.affiliation: Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. zhao5@illinois.edu.|Departments of Chemistry, Biochemistry, and Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. zhao5@illinois.edu.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000465:Algorithms; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D003198:Computer Simulation; D060847:Metabolic Engineering / Q000379:methods*
keywords: Cell factories; DNA assembly; Metabolic engineering; Pathway construction; Pathway design; Pathway optimization; Synthetic biology