title: Osteopontin Promotes Expression of Matrix Metalloproteinase 13 through NF-κB Signaling in Osteoarthritis.
abstract: Osteopontin (OPN) is associated with the severity and progression of osteoarthritis (OA); however, the mechanism of OPN in the pathogenesis of OA is unknown. In this study, we found that OA patients had higher abundance of OPN and matrix metalloproteinase 13 (MMP13). In chondrocytes, we showed that OPN promoted the production of MMP13 and activation of NF-κB pathway by increasing the abundance of p65 and phosphorylated p65 and translocation of p65 protein from cytoplasm to nucleus. Notably, inhibition of NF-κB pathway by inhibitor suppressed the production of MMP13 induced by OPN treatment. In conclusion, OPN induces production of MMP13 through activation of NF-κB pathway.
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Bone and Joint, The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen 518020, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Bone and Joint, The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen 518020, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Zhenhan
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zeng
authors.affiliation: Department of Orthopaedics, Second People's Hospital of Jingmen, Jingmen 448000, China.
authors.forename: Min
authors.initials: M
authors.lastname: Tu
authors.affiliation: Department of Joint Surgery, Changsha Central Hospital, Changsha 410000, China.
authors.forename: Liangjun
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.affiliation: Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Guanghua
authors.identifier: 0000-0001-9230-9053
authors.initials: G
authors.lastname: Lei