title: Hybrid bright-field and hologram imaging of cell dynamics.
abstract: Volumetric observation is essential for understanding the details of complex biological phenomena. In this study, a bright-field microscope, which provides information on a specific 2D plane, and a holographic microscope, which provides information spread over 3D volumes, are integrated to acquire two complementary images simultaneously. The developed system was successfully applied to capture distinct T-cell adhesion dynamics on inflamed endothelial layers, including capture, rolling, crawling, transendothelial migration, and subendothelial migration.
authors.affiliation: Center for Biofluid and Biomimic Research, Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.
authors.forename: Hyeokjun
authors.initials: H
authors.lastname: Byeon
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.
authors.forename: Jaehyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Center for Biofluid and Biomimic Research, Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.|School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.
authors.forename: Junsang
authors.initials: J
authors.lastname: Doh
authors.affiliation: Center for Biofluid and Biomimic Research, Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.|School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.
authors.forename: Sang Joon
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000295:instrumentation / Q000379:methods; D006696:Holography; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D051379:Mice; D008822:Mice, Transgenic; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods