title: Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation inDrosophila.
abstract: Ionotropic Receptors (IRs) are a large subfamily of variant ionotropic glutamate receptors present across Protostomia. While these receptors are most extensively studied for their roles in chemosensory detection, recent work has implicated two family members, IR21a and IR25a, in thermosensation inDrosophila. Here we characterize one of the most evolutionarily deeply conserved receptors, IR93a, and show that it is co-expressed and functions with IR21a and IR25a to mediate physiological and behavioral responses to cool temperatures. IR93a is also co-expressed with IR25a and a distinct receptor, IR40a, in a discrete population of sensory neurons in the sacculus, a multi-chambered pocket within the antenna. We demonstrate that this combination of receptors is required for neuronal responses to dry air and behavioral discrimination of humidity differences. Our results identify IR93a as a common component of molecularly and cellularly distinct IR pathways important for thermosensation and hygrosensation in insects.
authors.affiliation: Department of Biology, National Center for Behavioral Genomics and Volen Center for Complex Systems, Brandeis University, Waltham, United States.
authors.forename: Zachary A
authors.initials: ZA
authors.lastname: Knecht
authors.affiliation: Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
authors.forename: Ana F
authors.initials: AF
authors.lastname: Silbering
authors.affiliation: Department of Biology, National Center for Behavioral Genomics and Volen Center for Complex Systems, Brandeis University, Waltham, United States.
authors.forename: Lina
authors.initials: L
authors.lastname: Ni
authors.affiliation: Department of Physics and Center for Brain Science, Harvard University, Cambridge, United States.|Department of Physics, University of Miami, Coral Gables, United States.
authors.forename: Mason
authors.initials: M
authors.lastname: Klein
authors.affiliation: Department of Biology, National Center for Behavioral Genomics and Volen Center for Complex Systems, Brandeis University, Waltham, United States.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Budelli
authors.affiliation: Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
authors.forename: Rati
authors.initials: R
authors.lastname: Bell
authors.affiliation: Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
authors.forename: Liliane
authors.initials: L
authors.lastname: Abuin
authors.affiliation: Department of Physics, University of Miami, Coral Gables, United States.
authors.forename: Anggie J
authors.initials: AJ
authors.lastname: Ferrer
authors.affiliation: Department of Physics and Center for Brain Science, Harvard University, Cambridge, United States.
authors.forename: Aravinthan Dt
authors.initials: AD
authors.lastname: Samuel
authors.affiliation: Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
authors.forename: Richard
authors.initials: R
authors.lastname: Benton
authors.affiliation: Department of Biology, National Center for Behavioral Genomics and Volen Center for Complex Systems, Brandeis University, Waltham, United States.
authors.forename: Paul A
authors.identifier: 0000-0002-8274-6564
authors.initials: PA
authors.lastname: Garrity
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D003080:Cold Temperature; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D006813:Humidity; D008565:Membrane Proteins; D058468:Receptors, Ionotropic Glutamate / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D008565:Membrane Proteins; D058468:Receptors, Ionotropic Glutamate; C098676:in protein, Drosophila
keywords: D. melanogaster; Ir21a; Ir25a; Ir40a; Ir93a; dry sensation; ionotropic receptor; neuroscience