title: Mesenchymal Stem Cells from Human Amniotic Membrane and Umbilical Cord Can Diminish Immunological Response in an in vitro Allograft Model.
abstract: OBJECTIVE
  Mesenchymal stem cells (MSCs) are gaining rising interest in gynecology and obstetrics. MSCs immunomodulatory properties are suitable enough to reduce perinatal morbidity caused by inflammation in premature neonates. The aim of this study was to evaluate and compare the ability to inhibit allo-activated lymphocytes proliferation by MSCs derived from different sources: amniotic membrane (AM), umbilical cord (UC) and adipose tissue (AT).
  
  
  METHODS
  MSCs were isolated from AM (n = 7) and UC (n = 6) and AT (n = 6) of healthy women. Cells were characterized by flow cytometry and differentiation assay. To evaluate the potential of fetal and adult MSCs to diminish immunological response, mixed lymphocytes reaction (MLR) was performed.
  
  
  RESULTS
  Amnion and UC-derived cells displayed typical MSCs characteristics. Addition of MSCs to MLR significantly inhibited the proliferation of stimulated lymphocytes. The effect was observed regardless of the MSCs type used (p < 0.01 in all groups). Comparative analysis revealed no significant differences in this action between tested MSCs types. Additionally, no type of MSCs significantly stimulated allogeneic lymphocytes.
  
  
  CONCLUSIONS
  The results prove the immunosuppressive capacities of fetal-derived MSCs in vitro. In the future, they may be potentially used to treat premature newborn as well as in immunomodulation in post-transplant therapy.
authors.affiliation: Department of Obstetrics and Gynecology, The Medical University of Warsaw, Warsaw, Poland.
authors.forename: Filip A
authors.initials: FA
authors.lastname: Dabrowski
authors.forename: Anna
authors.initials: A
authors.lastname: Burdzinska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kulesza
authors.forename: Marcin
authors.initials: M
authors.lastname: Chlebus
authors.forename: Beata
authors.initials: B
authors.lastname: Kaleta
authors.forename: Jan
authors.initials: J
authors.lastname: Borysowski
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Zolocinska
authors.forename: Leszek
authors.initials: L
authors.lastname: Paczek
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Wielgos
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000328:Adult; D064591:Allografts / Q000276:immunology*; D000650:Amnion / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D056747:Immunomodulation / Q000276:immunology; D007231:Infant, Newborn; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D011247:Pregnancy; D014470:Umbilical Cord / Q000166:cytology*
keywords: Allotransplantation; Amnion; Immunomodulation; Mesenchymal stem cells; Mixed leukocyte reaction; Umbilical cord