title: HLA-F and MHC-I Open Conformers Bind Natural Killer Cell Ig-Like Receptor KIR3DS1.
abstract: Based on previous findings supporting HLA-F as a ligand for KIR3DL2 and KIR2DS4, we investigated the potential for MHC-I open conformers (OCs) as ligands for KIR3DS1 and KIR3DL1 through interactions measured by surface plasmon resonance. These measurements showed physical binding of KIR3DS1 but not KIR3DL1 with HLA-F and other MHC-I OC while also confirming the allotype specific binding of KIR3DL1 with MHC-I peptide complex. Concordant results were obtained with biochemical pull-down from cell lines and biochemical heterodimerization experiments with recombinant proteins. In addition, surface binding of HLA-F and KIR3DS1 to native and activated NK and T cells was coincident with specific expression of the putative ligand or receptor. A functional response of KIR3DS1 was indicated by increased granule exocytosis in activated cells incubated with HLA-F bound to surfaces. The data extend a model for interaction between MHC-I open conformers and activating KIR receptors expressed during an inflammatory response, potentially contributing to communication between the innate and adaptive immune response.
authors.affiliation: The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA, 98109, United States of America.
authors.forename: Aura
authors.initials: A
authors.lastname: Burian
authors.affiliation: The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA, 98109, United States of America.
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Wang
authors.affiliation: The Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA, 98109, United States of America.
authors.forename: Kathryn A K
authors.initials: KA
authors.lastname: Finton
authors.affiliation: The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA, 98109, United States of America.
authors.forename: Ni
authors.initials: N
authors.lastname: Lee
authors.affiliation: Nara Medical University, Kashihara, Nara 634, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Ishitani
authors.affiliation: The Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA, 98109, United States of America.
authors.forename: Roland K
authors.initials: RK
authors.lastname: Strong
authors.affiliation: The Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA, 98109, United States of America.
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Geraghty
mesh_terms: D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008958:Models, Molecular; D011485:Protein Binding; D017510:Protein Folding; D054349:Receptors, KIR3DS1 / Q000378:metabolism*
chemical_list: C062341:HLA-F antigens; D015395:Histocompatibility Antigens Class I; D054349:Receptors, KIR3DS1