title: Digital Gene Expression Profiling to Explore Differentially Expressed Genes Associated with Terpenoid Biosynthesis during Fruit Development in Litsea cubeba.
abstract: Mountain pepper (Litseacubeba(Lour.) Pers.) (Lauraceae) is an important industrial crop as an ingredient in cosmetics, pesticides, food additives and potential biofuels. These properties are attributed to monoterpenes and sesquiterpenes. However, there is still no integrated model describing differentially expressed genes (DEGs) involved in terpenoid biosynthesis during the fruit development ofL. cubeba. Here, we performed digital gene expression (DGE) using the Illumina NGS platform to evaluated changes in gene expression during fruit development inL. cubeba. DGE generated expression data for approximately 19354 genes. Fruit at 60 days after flowering (DAF) served as the control, and a total of 415, 1255, 449 and 811 up-regulated genes and 505, 1351, 1823 and 1850 down-regulated genes were identified at 75, 90, 105 and 135 DAF, respectively. Pathway analysis revealed 26 genes involved in terpenoid biosynthesis pathways. Three DEGs had continued increasing or declining trends during the fruit development. The quantitative real-time PCR (qRT-PCR) results of five differentially expressed genes were consistent with those obtained from Illumina sequencing. These results provide a comprehensive molecular biology background for research on fruit development, and information that should aid in metabolic engineering to increase the yields ofL. cubebaessential oil.
authors.affiliation: State Key Laboratory of Forest Genetics and Tree Breeding, Chinese Academy of Forestry, No.1 Dong Xiaofu, Beijing 10086, China. minggao1984@caf.ac.cn.|Research Institute of Subtropical Forestry, Chinese Academy of Forestry, No.73 Daqiao Road, Hangzhou 311400, Zhejiang Province, China. minggao1984@caf.ac.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Forest Genetics and Tree Breeding, Chinese Academy of Forestry, No.1 Dong Xiaofu, Beijing 10086, China. linkeyuanxl@126.com.|Research Institute of Subtropical Forestry, Chinese Academy of Forestry, No.73 Daqiao Road, Hangzhou 311400, Zhejiang Province, China. linkeyuanxl@126.com.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Forest Genetics and Tree Breeding, Chinese Academy of Forestry, No.1 Dong Xiaofu, Beijing 10086, China. yicun_chen@163.com.|Research Institute of Subtropical Forestry, Chinese Academy of Forestry, No.73 Daqiao Road, Hangzhou 311400, Zhejiang Province, China. yicun_chen@163.com.
authors.forename: Yicun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Forest Genetics and Tree Breeding, Chinese Academy of Forestry, No.1 Dong Xiaofu, Beijing 10086, China. wyd11111@126.com.|Research Institute of Subtropical Forestry, Chinese Academy of Forestry, No.73 Daqiao Road, Hangzhou 311400, Zhejiang Province, China. wyd11111@126.com.
authors.forename: Yangdong
authors.initials: Y
authors.lastname: Wang
keywords: Litsea cubeba (Lour.) Pers.; differentially expressed genes; digital gene expression; quantitative real-time PCR; terpenoid biosynthesis