title: Multiplex Gene Expression Profiling of 16 Target Genes in Neoplastic and Non-Neoplastic Canine Mammary Tissues Using Branched-DNA Assay.
abstract: Mammary gland tumors are one of the most common neoplasms in female dogs, and certain breeds are prone to develop the disease. The use of biomarkers in canines is still restricted to research purposes. Therefore, the necessity to analyze gene profiles in different mammary entities in large sample sets is evident in order to evaluate the strength of potential markers serving as future prognostic factors. The aim of the present study was to analyze the gene expression of 16 target genes (BRCA1,BRCA2,FOXO3,GATA4,HER2,HMGA1,HMGA2,HMGB1,MAPK1,MAPK3,MCL1,MYC,PFDN5,PIK3CA,PTEN, andTP53) known to be involved in human and canine mammary neoplasm development. Expression was analyzed in 111 fresh frozen (FF) and in 170 formalin-fixed, paraffin-embedded (FFPE) specimens of neoplastic and non-neoplastic canine mammary tissues using a multiplexed branched-DNA (b-DNA) assay.TP53,FOXO3,PTEN, andPFDN5expression revealed consistent results with significant low expression in malignant tumors. The possibility of utilizing them as predictive factors as well as for assisting in the choice of an adequate gene therapy may help in the development of new and improved approaches in canine mammary tumors.
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover D-30559, Germany. florenza@ripoli.com.br.|Hematology Oncology and Palliative Medicine, Clinic III, University of Rostock, Rostock D-18057, Germany. florenza@ripoli.com.br.
authors.forename: Florenza
authors.initials: F
authors.lastname: Lüder Ripoli
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover D-30559, Germany. Susanne.Conradine.Hammer@tiho-hannover.de.|Hematology Oncology and Palliative Medicine, Clinic III, University of Rostock, Rostock D-18057, Germany. Susanne.Conradine.Hammer@tiho-hannover.de.
authors.forename: Susanne
authors.initials: S
authors.lastname: Conradine Hammer
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover D-30559, Germany. annika.mohr@tiho-hannover.de.|Hematology Oncology and Palliative Medicine, Clinic III, University of Rostock, Rostock D-18057, Germany. annika.mohr@tiho-hannover.de.
authors.forename: Annika
authors.initials: A
authors.lastname: Mohr
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover D-30559, Germany. willenbrock@preclinics.com.
authors.forename: Saskia
authors.initials: S
authors.lastname: Willenbrock
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover, Hannover D-30559, Germany. marion.hewicker-trautwein@tiho-hannover.de.
authors.forename: Marion
authors.initials: M
authors.lastname: Hewicker-Trautwein
authors.affiliation: Institute of Veterinary Medicine, Georg-August-University Göttingen, Göttingen D-37077, Germany. bbrenig@gwdg.de.
authors.forename: Bertram
authors.initials: B
authors.lastname: Brenig
authors.affiliation: Hematology Oncology and Palliative Medicine, Clinic III, University of Rostock, Rostock D-18057, Germany. hugo.murua.escobar@med.uni-rostock.de.
authors.forename: Hugo
authors.initials: H
authors.lastname: Murua Escobar
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover D-30559, Germany. ingo.nolte@tiho-hannover.de.
authors.forename: Ingo
authors.initials: I
authors.lastname: Nolte
keywords: RNA; canine mammary tumor; formalin-fixed; fresh frozen tissue; gene expression; multiplexed branched-DNA (b-DNA) assay; paraffin-embedded samples