title: Cell type-specific long-range connections of basal forebrain circuit.
abstract: The basal forebrain (BF) plays key roles in multiple brain functions, including sleep-wake regulation, attention, and learning/memory, but the long-range connections mediating these functions remain poorly characterized. Here we performed whole-brain mapping of both inputs and outputs of four BF cell types - cholinergic, glutamatergic, and parvalbumin-positive (PV+) and somatostatin-positive (SOM+) GABAergic neurons - in the mouse brain. Using rabies virus -mediated monosynaptic retrograde tracing to label the inputs and adeno-associated virus to trace axonal projections, we identified numerous brain areas connected to the BF. The inputs to different cell types were qualitatively similar, but the output projections showed marked differences. The connections to glutamatergic and SOM+ neurons were strongly reciprocal, while those to cholinergic and PV+ neurons were more unidirectional. These results reveal the long-range wiring diagram of the BF circuit with highly convergent inputs and divergent outputs and point to both functional commonality and specialization of different BF cell types.
authors.affiliation: Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, Berkeley, United States.
authors.forename: Johnny Phong
authors.initials: JP
authors.lastname: Do
authors.affiliation: Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, Berkeley, United States.
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.affiliation: Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, Berkeley, United States.
authors.forename: Seung-Hee
authors.identifier: 0000-0002-9486-5771
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, Berkeley, United States.
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Chang
authors.affiliation: Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, Berkeley, United States.
authors.forename: Siyu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, Berkeley, United States.
authors.forename: Shinjae
authors.initials: S
authors.lastname: Chung
authors.affiliation: Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, Berkeley, United States.
authors.forename: Tyler J
authors.identifier: 0000-0002-8361-837X
authors.initials: TJ
authors.lastname: Yung
authors.affiliation: Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, Berkeley, United States.
authors.forename: Jiang Lan
authors.initials: JL
authors.lastname: Fan
authors.affiliation: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Miyamichi
authors.affiliation: Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States.
authors.forename: Liqun
authors.initials: L
authors.lastname: Luo
authors.affiliation: Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, Berkeley, United States.
authors.forename: Yang
authors.identifier: 0000-0002-3818-877X
authors.initials: Y
authors.lastname: Dan
mesh_terms: D000818:Animals; D066187:Basal Forebrain / Q000033:anatomy & histology*; D001931:Brain Mapping; D000229:Dependovirus / Q000235:genetics; D051379:Mice; D009434:Neural Pathways / Q000033:anatomy & histology*; D011820:Rabies virus / Q000235:genetics
keywords: anatomy; basal forebrain; cholinergic; mouse; neuroscience; parvalbumin; rabies virus; somatostatin