title: Profiling of adhesive-related genes in the freshwater cnidarian Hydra magnipapillata by transcriptomics and proteomics.
abstract: The differentiated ectodermal basal disc cells of the freshwater cnidarian Hydra secrete proteinaceous glue to temporarily attach themselves to underwater surfaces. Using transcriptome sequencing and a basal disc-specific RNA-seq combined with in situ hybridisation a highly specific set of candidate adhesive genes was identified. A de novo transcriptome assembly of 55,849 transcripts (>200 bp) was generated using paired-end and single reads from Illumina libraries constructed from different polyp conditions. Differential transcriptomics and spatial gene expression analysis by in situ hybridisation allowed the identification of 40 transcripts exclusively expressed in the ectodermal basal disc cells. Comparisons after mass spectrometry analysis of the adhesive secretion showed a total of 21 transcripts to be basal disc specific and eventually secreted through basal disc cells. This is the first study to survey adhesion-related genes in Hydra. The candidate list presented in this study provides a platform for unravelling the molecular mechanism of underwater adhesion of Hydra.
authors.affiliation: a Institute of Zoology and Center for Molecular Biosciences Innsbruck , University of Innsbruck , Innsbruck , Austria.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Rodrigues
authors.affiliation: a Institute of Zoology and Center for Molecular Biosciences Innsbruck , University of Innsbruck , Innsbruck , Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Ostermann
authors.affiliation: b Division of Clinical Biochemistry, Biocenter , Innsbruck Medical University , Innsbruck , Austria.
authors.forename: Leopold
authors.initials: L
authors.lastname: Kremeser
authors.affiliation: b Division of Clinical Biochemistry, Biocenter , Innsbruck Medical University , Innsbruck , Austria.
authors.forename: Herbert
authors.initials: H
authors.lastname: Lindner
authors.affiliation: c D-BSSE , ETH Zürich , Basel , Switzerland.
authors.forename: Christian
authors.initials: C
authors.lastname: Beisel
authors.affiliation: d ERIBA , University of Groningen, University Medical Center Groningen , Groningen , The Netherlands.
authors.forename: Eugene
authors.initials: E
authors.lastname: Berezikov
authors.affiliation: a Institute of Zoology and Center for Molecular Biosciences Innsbruck , University of Innsbruck , Innsbruck , Austria.
authors.forename: Bert
authors.initials: B
authors.lastname: Hobmayer
authors.affiliation: a Institute of Zoology and Center for Molecular Biosciences Innsbruck , University of Innsbruck , Innsbruck , Austria.
authors.forename: Peter
authors.initials: P
authors.lastname: Ladurner
keywords: Hydra; basal disc; bioadhesion; differential transcriptome; proteome; transcriptome