title: The natural plant stress elicitor cis-jasmone causes cultivar-dependent reduction in growth of the stink bug, Euschistus heros and associated changes in flavonoid concentrations in soybean, Glycine max.
abstract: To test the hypothesis that the plant stress related elicitor cis-jasmone (cJ) provides protection in soybean pods against the seed-sucking stink bug pest, Euschistus heros, the growth of E. heros on cJ-treated pods was investigated using three soybean cultivars differing in insect susceptibility, i.e. BRS 134 (susceptible), IAC 100 (resistant) and Dowling (resistant). E. heros showed reduced weight gain when fed cJ-treated Dowling, whereas no effect on weight gain was observed when fed other treated cultivars. Using analysis of variance, a three factor (cultivar x treatment x time) interaction was observed with concentrations of the flavonoid glycosides daidzin and genistin, and their corresponding aglycones, daidzein and genistein. There were increases in genistein and genistin concentrations in cJ-treated Dowling at 144 and 120 h post treatment, respectively. Higher concentrations of malonyldaidzin and malonylgenistin in Dowling, compared to BRS 134 and IAC 100, were observed independently of time, the highest concentrations being observed in cJ-treated seeds. Levels of glycitin and malonylglycitin were higher in BRS 134 and IAC 100 compared to Dowling. Canonical variate analysis indicated daidzein (in the first two canonical variates) and genistein (in the first only) as important discriminatory variables. These results suggest that cJ treatment leads to an increase in the levels of potentially defensive isoflavonoids in immature soybean seeds, but the negative effect upon E. heros performance is cultivar-dependent.
authors.affiliation: Embrapa Centro Nacional de Pesquisa de Soja, Caixa Postal: 231, CEP. 86001-970, Londrina, PR, Brazil; UNESP Universidade Estadual Paulista, FCAV, Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP. 14884-900, Jaboticabal, SP, Brazil.
authors.forename: José P
authors.initials: JP
authors.lastname: da Graça
authors.affiliation: Embrapa Centro Nacional de Pesquisa de Soja, Caixa Postal: 231, CEP. 86001-970, Londrina, PR, Brazil; UEL Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, Caixa Postal 6001, CEP. 86051-980, Londrina, PR, Brazil.
authors.forename: Tatiana E
authors.initials: TE
authors.lastname: Ueda
authors.affiliation: Embrapa Centro Nacional de Pesquisa de Soja, Caixa Postal: 231, CEP. 86001-970, Londrina, PR, Brazil; UEM Universidade Estadual de Maringá, Avenida Colombo, 5790, Jardim Universitario, CEP. 87020-900, Maringá, PR, Brazil.
authors.forename: Tatiani
authors.initials: T
authors.lastname: Janegitz
authors.affiliation: Embrapa Centro Nacional de Pesquisa de Soja, Caixa Postal: 231, CEP. 86001-970, Londrina, PR, Brazil; IAC Instituto Agronômico de Campinas, Av. Barão de Itapura, 1481, Cx. Postal: 28, CEP. 13012-970, Campinas, SP, Brazil.
authors.forename: Simone S
authors.initials: SS
authors.lastname: Vieira
authors.affiliation: Embrapa Centro Nacional de Pesquisa de Soja, Caixa Postal: 231, CEP. 86001-970, Londrina, PR, Brazil; UEL Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, Caixa Postal 6001, CEP. 86051-980, Londrina, PR, Brazil.
authors.forename: Mariana C
authors.initials: MC
authors.lastname: Salvador
authors.affiliation: Embrapa Centro Nacional de Pesquisa de Soja, Caixa Postal: 231, CEP. 86001-970, Londrina, PR, Brazil.
authors.forename: Maria C N
authors.initials: MC
authors.lastname: de Oliveira
authors.affiliation: UNAERP Universidade de Ribeirão Preto, Avenida Costábile Romano, Caixa Postal: 2201, CEP. 14096-900, Ribeirão Preto, SP, Brazil.
authors.forename: Sonia M
authors.initials: SM
authors.lastname: Zingaretti
authors.affiliation: Computational and Systems Biology Department, Rothamsted Research, Harpenden, Herts. AL5 2JQ, United Kingdom.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Powers
authors.affiliation: Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, Herts. AL5 2JQ, United Kingdom.
authors.forename: John A
authors.initials: JA
authors.lastname: Pickett
authors.affiliation: Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, Herts. AL5 2JQ, United Kingdom.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Birkett
authors.affiliation: Embrapa Centro Nacional de Pesquisa de Soja, Caixa Postal: 231, CEP. 86001-970, Londrina, PR, Brazil. Electronic address: clarabeatriz.campo@embrapa.br.
authors.forename: Clara B
authors.initials: CB
authors.lastname: Hoffmann-Campo
mesh_terms: D000818:Animals; D003517:Cyclopentanes / Q000494:pharmacology*; D005247:Feeding Behavior / Q000187:drug effects; D005419:Flavonoids / Q000032:analysis; D019833:Genistein / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D020062:Heteroptera / Q000187:drug effects*; D007529:Isoflavones / Q000032:analysis; D054883:Oxylipins / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry* / Q000469:parasitology
chemical_list: D003517:Cyclopentanes; D005419:Flavonoids; D005960:Glucosides; D007529:Isoflavones; D054883:Oxylipins; C040641:genistin; C521200:malonylgenistin; C013908:daidzin; C459793:6''-O-malonyldaidzin; C004742:daidzein; D019833:Genistein; C433283:glycitin; C086299:jasmone
keywords: Defence; Development; Euschistus heros; Flavonoid; Glycine max; cis-jasmone