title: MicroRNA‑187 is an independent prognostic factor in lung cancer and promotes lung cancer cell invasion via targeting of PTRF.
abstract: MicroRNAs (miRNAs) are involved in the progression of different types of cancers giving new hope for cancer treatment. The role and regulatory mechanism of microRNA‑187 (miR‑187) are largely unknown. In the present study, 74 patients with non‑small cell lung cancer (NSCLC) were selected. Tumor tissues and matched normal tissues were collected for determining the expression level of miR‑187. Cell research was performed to detect the function of miR‑187. The expression level was measured and miR‑187 was found to be overexpressed in the NSCLC cell lines and tissues. Overexpression of miR‑187 promoted cell proliferation in the A549 and H1650 cell lines. Moreover, overexpression of miR‑187 also promoted cell migration and invasion. Polymerase I and transcript release factor (PTRF) was identified as a target of miR‑187. Overexpression of miR‑187 suppressed the expression of PTRF. Knockdown of PTRF promoted lung cancer cell invasion, and overexpression of PTRF had a negative effect on lung cancer cell invasion. The PTRF messenger RNA (mRNA) levels in cancer tissues were significantly lower than those in their adjacent normal lung tissues as determined by real‑time PCR (RT‑PCR). The expression of the PTRF protein was significantly weaker than that in the adjacent normal lung tissues using immunohistochemical staining. The findings revealed that miR‑187 promotes cell growth and invasion by targeting PTRF and miR‑187 may be a new prognostic factor for NSCLC.
authors.affiliation: Department of Stomatology, Wuhan Medical and Healthcare Center for Women and Children, Wuhan, Hubei 430015, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Peng
authors.affiliation: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Hong-Zhou
authors.initials: HZ
authors.lastname: Liu
authors.affiliation: Department of Second Oncology Surgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Stomatology, Wuhan Medical and Healthcare Center for Women and Children, Wuhan, Hubei 430015, P.R. China.
authors.forename: Zhuo-Feng
authors.initials: ZF
authors.lastname: Deng
authors.affiliation: Department of Stomatology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Chao-Rong
authors.initials: CR
authors.lastname: Tie
authors.affiliation: Department of Stomatology, Wuhan Medical and Healthcare Center for Women and Children, Wuhan, Hubei 430015, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Rao
authors.affiliation: Department of Stomatology, Wuhan Medical and Healthcare Center for Women and Children, Wuhan, Hubei 430015, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Stomatology, Wuhan Medical and Healthcare Center for Women and Children, Wuhan, Hubei 430015, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: You
authors.affiliation: Department of Stomatology, Wuhan Medical and Healthcare Center for Women and Children, Wuhan, Hubei 430015, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Stomatology, Wuhan Medical and Healthcare Center for Women and Children, Wuhan, Hubei 430015, P.R. China.
authors.forename: Chuan-Bao
authors.initials: CB
authors.lastname: Cai
authors.affiliation: Department of Stomatology, Wuhan Medical and Healthcare Center for Women and Children, Wuhan, Hubei 430015, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Department of Stomatology, Wuhan Medical and Healthcare Center for Women and Children, Wuhan, Hubei 430015, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Stomatology, Wuhan Medical and Healthcare Center for Women and Children, Wuhan, Hubei 430015, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Wuhan Medical and Healthcare Center for Women and Children, Wuhan, Hubei 430015, P.R. China.
authors.forename: Zhi-Yun
authors.initials: ZY
authors.lastname: Lei
mesh_terms: D000072283:A549 Cells; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D011379:Prognosis; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C497835:CAVIN1 protein, human; C571843:MIRN187 microRNA, human; D035683:MicroRNAs; D016601:RNA-Binding Proteins