title: Alkaline phosphatase and OCT-3/4 as useful markers for predicting susceptibility of human deciduous teeth-derived dental pulp cells to reprogramming factor-induced iPS cells.
abstract: OBJECTIVE
  The aim of the present study was to prove that primary cells enriched with stem cells are more easily reprogrammed to generate induced pluripotent stem (iPS) cells than those with scarce numbers of stem cells.
  
  
  METHODS
  We surveyed the alkaline phosphatase (ALP) activity in five primarily-isolated human deciduous teeth-derived dental pulp cells (HDDPC) with cytochemical staining to examine the possible presence of stem cells. Next, the expression of stemness-specific factors, such as OCT(Octumer-binding transcription factor)3/4, NANOG, SOX2(SRY (sex determining region Y)-box 2), CD90, muscle segment homeodomain homeobox (MSX) 1, and MSX2, was assessed with a reverse transcription polymerase chain reaction method. Finally, these isolated HDDPC were transfected with plasmids carrying genes coding Yamanaka factors to determine whether these cells could be reprogrammed to generate iPS cells.
  
  
  RESULTS
  Of the five primarily-isolated HDDPC, two (HDDPC-1 and -5) exhibited higher degrees of ALP activity. OCT-3/4 expression was also prominent in those two lines. Furthermore, these two lines proliferated faster than the other three lines. The transfection of HDDPC with Yamanaka factors resulted in the generation of iPS cells from HDDPC-1 and -5.
  
  
  CONCLUSIONS
  The number of cells with the stemness property of HDDPC differs among individuals, which suggests that HDDPC showing an increased expression of both ALP and OCT-3/4 can be more easily reprogrammed to generate iPS cells after the forced expression of reprogramming factors.
authors.affiliation: Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Inada
authors.affiliation: Department of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan.
authors.forename: Issei
authors.initials: I
authors.lastname: Saitoh
authors.affiliation: Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kubota
authors.affiliation: Department of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Soda
authors.affiliation: Department of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Matsueda
authors.affiliation: Department of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Murakami
authors.affiliation: Department of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sawami
authors.affiliation: Department of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kagoshima
authors.affiliation: Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Youichi
authors.initials: Y
authors.lastname: Yamasaki
authors.affiliation: Frontier Science Research Center, Kagoshima University, Kagoshima, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sato
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D057026:Induced Pluripotent Stem Cells; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis*; D014094:Tooth, Deciduous
chemical_list: D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D000469:Alkaline Phosphatase
keywords: OCT-3/4; alkaline phosphatase; deciduous teeth; dental pulp cell; induced pluripotent stem cell