title: Isolation and biochemical characterization of a gamma-type phospholipase A2inhibitor from Macropisthodon rudis snake serum.
abstract: A novel phospholipaseA2(PLA2) inhibitory protein (PLI) was purified from the serum of Macropisthodon rudis, a non-venomous snake mainly found in southern China. The molecular mass of the purified PLI was 160 kDa as determined by Superdex 200HR; however, the PLI protein had only one subunit of 25.4 kDa as determined by 12% SDS-PAGE, indicating an oligomeric protein. PLI cDNA obtained by PCR from the liver of Macropisthodon rudis, revealed 549 bps coding for a mature protein of 183 amino acid residues. Based on an amino acid sequence alignment with venomous and non-venomous snakes, this inhibitor was determined to be in the γ type family of PLI. In vitro experiments showed that PLIγ inhibited enzymatic, inflammatory, and antibacterial activities of snake venom PLA2isolated from Agkistrodon acutus.
authors.affiliation: Department of Clinical Laboratory, The Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330003, PR China. Electronic address: zhongliborn@yahoo.com.
authors.forename: Lipeng
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Department of Biochemistry, College of Basic Medical Science, Nanchang University, Nanchang, Jiangxi, 330006, PR China. Electronic address: merlyhuang@sohu.com.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D017812:Colubridae / Q000097:blood*; D018076:DNA, Complementary; D064801:Phospholipase A2 Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D064801:Phospholipase A2 Inhibitors
keywords: Agkistrodon acutus; Antibacterial; Inflammation; Macropisthodon rudis; Snake venom phospholipaseA(2); phospholipaseA(2) inhibitors