title: Enhanced acute anti-inflammatory effects of CORM-2-loaded nanoparticles via sustained carbon monoxide delivery.
abstract: The aim of this study was to enhance the anti-inflammatory effects of carbon monoxide (CO) via sustained release of CO from carbon monoxide-releasing molecule-2-loaded lipid nanoparticles (CORM-2-NPs). CORM-2-NPs were prepared by hot high pressure homogenization method using trilaurin as a solid lipid core and Tween 20/Span 20/Myrj S40 as surfactant mixture. The physicochemical properties of CORM-2-NPs were characterized and CO release from CORM-2-NPs was assessed by myoglobin assay. In vitro anti-inflammatory effects were evaluated by nitric oxide assay in lipopolysaccharide-stimulated RAW 264.7 macrophages. In vivo anti-inflammatory activity was investigated by measuring paw volumes and histological examination in carrageenan-induced rat paw edema. Spherical CORM-2-NPs were around 100nm with narrow particle size distribution. The sustained CO release from CORM-2-NPs was observed and the half-life of CO release increased up to 10 times compared with CORM-2 solution. CORM-2-NPs showed enhanced in vitro anti-inflammatory effects by inhibition of nitric oxide production. Edema volume in rat paw was significantly reduced after treatment with CORM-2-NPs. Taken together, CORM-2-NPs have a great potential for CO therapeutics against inflammation via sustained release of CO.
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi 15588, Republic of Korea.
authors.forename: Omer Salman
authors.initials: OS
authors.lastname: Qureshi
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi 15588, Republic of Korea.
authors.forename: Alam
authors.initials: A
authors.lastname: Zeb
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi 15588, Republic of Korea.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Akram
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi 15588, Republic of Korea.
authors.forename: Myung-Sic
authors.initials: MS
authors.lastname: Kim
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi 15588, Republic of Korea.
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Kang
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi 15588, Republic of Korea.
authors.forename: Hoo-Seong
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Sheffield Institute for Translational Neuroscience, University of Sheffield, Sheffield, England, United Kingdom.
authors.forename: Arshad
authors.initials: A
authors.lastname: Majid
authors.affiliation: Department of Neurosurgery, CHA University, CHA Bundang Medical Center, Seongnam, Gyeonggi 13496, Republic of Korea.
authors.forename: Inbo
authors.initials: I
authors.lastname: Han
authors.affiliation: College of Pharmacy, Ajou University, Suwon, Gyeonggi 16499, Republic of Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Chang
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi 15588, Republic of Korea. Electronic address: onbae@hanyang.ac.kr.
authors.forename: Ok-Nam
authors.initials: ON
authors.lastname: Bae
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi 15588, Republic of Korea. Electronic address: jinkikim@hanyang.ac.kr.
authors.forename: Jin-Ki
authors.initials: JK
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry*; D002248:Carbon Monoxide / Q000737:chemistry*; D002470:Cell Survival; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D004487:Edema; D007249:Inflammation / Q000188:drug therapy; D008055:Lipids / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D046529:Microscopy, Electron, Transmission; D009211:Myoglobin / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D009569:Nitric Oxide / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D010316:Particle Size; D011312:Pressure; D000067996:RAW 264.7 Cells; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D004337:Drug Carriers; D008055:Lipids; D008070:Lipopolysaccharides; D009211:Myoglobin; D009942:Organometallic Compounds; D014280:Triglycerides; C447082:tricarbonyldichlororuthenium (II) dimer; D009569:Nitric Oxide; C004565:trilaurin; D002248:Carbon Monoxide
keywords: Anti-inflammatory effect; CORM-2; Carbon monoxide; Lipid nanoparticles; Sodium dithionite (PubChem CID: 24489); Sustained release; Tricarbonyldichlororuthenium(II) dimer (PubChem CID: 10951331); Trilaurin (PubChem CID: 10851); Tween 20 (PubChem CID: 443314)