title: Clinical implication of Keap1 and phosphorylated Nrf2 expression in hepatocellular carcinoma.
abstract: In this paper, variation tendency of phosphorylated Nrf2, as the activated form of native Nrf2, was studied in 107 primary hepatocellular carcinoma (HCC) specimens treated by curative hepatectomy. Moreover, the coexpression of oxidative stress markers Keap1 and pNrf2, and their association with pathological features were also evaluated based on those specimens. The results showed that preserved cytoplasmic Keap1 expression of cancer cells was observed in 59 HCCs, while reduced Keap1 expression was determined in remaining 48 ones. With regarding to nuclear pNrf2 expression, 75 HCCs were defined as high and the other 32 ones as low. There was a significant association between Keap1 and pNrf2 expression in HCCs. Higher pNrf2 expression was observed, at a more substantial proportion, in those specimens with reduced Keap1 expression, compared to those with preserved Keap1 expression. The subset with higher pNrf2 and reduced Keap1 expression was defined as pNrf2+Keap1-. According to the analysis of prognosis, this subset was significantly associated with poor 5-year overall survival and worse disease-free survival in HCCs, indicating that pNrf2 and Keap1 were two-functional biomolecules, not only the oxidative stress markers but also biomarkers for prognosis of HCCs.
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital of Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital of Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Yaojun
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital of Zhejiang University, Hangzhou, Zhejiang, China.|Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Ji
authors.affiliation: Department of Surgery, Zhejiang University Hospital, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital of Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of General Surgery, Zhejiang Putuo hospital, zhoushan, Zhejiang, China.
authors.forename: Feibo
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of General Surgery, Zhejiang Putuo hospital, zhoushan, Zhejiang, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Department of General Surgery, Zhejiang Putuo hospital, zhoushan, Zhejiang, China.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Kang
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital of Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Diyu
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital of Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital of Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
authors.affiliation: Department of General Surgery, Sir Run Run Shaw Hospital of Zhejiang University, Hangzhou, Zhejiang, China. cxjzu@hotmail.com.
authors.forename: Xiujun
authors.initials: X
authors.lastname: Cai
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000601:surgery; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006498:Hepatectomy; D006801:Humans; D000072019:Kelch-Like ECH-Associated Protein 1 / Q000235:genetics / Q000378:metabolism*; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011379:Prognosis; D055815:Young Adult
chemical_list: C431364:KEAP1 protein, human; D000072019:Kelch-Like ECH-Associated Protein 1; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human
keywords: Hepatocellular carcinoma; Keap1; Oxidative stress; phosphorylated Nrf2 (pNrf2); prognosis