title: Association of IGFBP-6 Expression with Metabolic Syndrome and Adiponectin and IGF-IR Receptor Levels in Colorectal Cancer.
abstract: OBJECTIVE
  To assess IGFBP-6 expression in relation with the presence of the metabolic syndrome, adiponectin receptors (AdipoR1 and AdipoR2) and IGF-IR levels in colorectal adenocarcinoma cases.
  
  
  METHODS
  IGFBP-6 mRNA and protein levels were analyzed using real-time quantitative PCR and Western blotting in 46 patients. ELISA and ow cytometry were used for evaluation of AdipoR1, AdipoR2 and IGF-IR.
  
  
  RESULTS
  The results showed that IGFBP-6 mRNA expression and the IGFBP-6 content were higher in tumor tissue samples of colorectal cancer patients with and without the metabolic syndrome. In addition, IGFBP-6 mRNA expression was associated with tumor invasion (tumor size) and the IGFBP-6 protein level was associated with nodal status. Positive correlations and positive nonlinear relations were found between the IGFBP-6 level and the AdipoR1 and AdipoR2 contents in colorectal cancer patients.
  
  
  CONCLUSIONS
  The IGFBP-6 mRNA level and protein level were found to be associated with presence of the metabolic syndrome. Positive correlations indicated probable cross-talk between the IGF-IR-mediated and adiponectin-mediated signaling pathways in colorectal carcinomas. IGFBP-6 may be considered as a potential biomarker associated with lymphogenous metastasis and the metabolic syndrome in colorectal cancer.
authors.affiliation: Tomsk Cancer Research Institute, Tomsk, Russian Federation, Tomsk, Russian E-mail : bochkarevanv@oncology.tomsk.ru.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Yunusova
authors.forename: Liudmila V
authors.initials: LV
authors.lastname: Spirina
authors.forename: Alena E
authors.initials: AE
authors.lastname: Frolova
authors.forename: Sergey G
authors.initials: SG
authors.lastname: Afanas'ev
authors.forename: Elena S
authors.initials: ES
authors.lastname: Kolegova
authors.forename: Irina V
authors.initials: IV
authors.lastname: Kondakova
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D052242:Adiponectin / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics; D015179:Colorectal Neoplasms / Q000473:pathology; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D018976:Insulin-Like Growth Factor Binding Protein 6 / Q000235:genetics*; D008297:Male; D024821:Metabolic Syndrome / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D017526:Receptor, IGF Type 1; D017451:Receptors, Somatomedin / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D052242:Adiponectin; D014408:Biomarkers, Tumor; C000597236:IGF1R protein, human; D018976:Insulin-Like Growth Factor Binding Protein 6; D012333:RNA, Messenger; D017451:Receptors, Somatomedin; D017526:Receptor, IGF Type 1