title: Deficiency of the DSPP-cleaving enzymes meprin α and meprin β does not result in dentin malformation in mice.
abstract: Formation of dentin requires the maturation of procollagen I and the proteolytic processing of the dentin sialophosphoprotein (DSPP). These cleavage events can be facilitated by the metalloproteinases meprin α and meprin β as well as by bone morphogenetic protein 1 (BMP-1). All three enzymes have been shown to play important roles during collagen I maturation in vivo and their potential in cleaving DSPP was demonstrated in vitro. Hence, it has been discussed whether meprin α, meprin β, BMP-1 or all three are crucial factors in the onset and progression of dentin-related diseases and this issue is addressed here. In this study, we compare the incisors and molars of meprin α (Mep1a-/-)- and meprin β (Mep1b-/-)-deficient mice with wild-type (WT) controls on the macroscopic and microscopic level. The dentin was evaluated towards the bone mineral density, dentin volume, calcification and collagen matrix integrity. Using immunohistochemistry, we could identify meprin β, BMP-1 and DSPP/DSP in the pre-dentin of WT mice. Nevertheless, no significant dentin malformation was observed in Mep1b-/-or Mep1a-/-deficient mice.
authors.affiliation: Anatomical Institute, Kiel University, Otto-Hahn-Platz 8, 24118, Kiel, Germany. p.arnold@anat.uni-kiel.de.
authors.forename: Philipp
authors.initials: P
authors.lastname: Arnold
authors.affiliation: Anatomical Institute, Kiel University, Otto-Hahn-Platz 8, 24118, Kiel, Germany.
authors.forename: Lara
authors.initials: L
authors.lastname: Koopmann
authors.affiliation: Biochemical Institute, Kiel University, Otto-Hahn-Platz 9, 24118, Kiel, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Peters
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital Schleswig-Holstein, Arnold-Heller-Str. 16, 24105, Kiel, Germany.
authors.forename: Falk
authors.initials: F
authors.lastname: Birkenfeld
authors.affiliation: Department of Tissue Biology and Therapeutic Engineering, Unité Mixte de Recherche 5305, Centre National de la Recherche Scientifique/University of Lyon, 69367, Lyon Cedex 7, France.
authors.forename: Sandrine Vadon-Le
authors.initials: SV
authors.lastname: Goff
authors.affiliation: Section Biomedical Imaging, Department of Radiology and Neurology, University Hospital Schleswig-Holstein, Am Botanischen Garten 18, 24118, Kiel, Germany.
authors.forename: Timo
authors.initials: T
authors.lastname: Damm
authors.affiliation: Department of Biomedical Sciences, Texas A&M University Baylor College of Dentistry, 3302 Gaston Avenue, 75246, Dallas, TX, USA.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Tissue Biology and Therapeutic Engineering, Unité Mixte de Recherche 5305, Centre National de la Recherche Scientifique/University of Lyon, 69367, Lyon Cedex 7, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Moali
authors.affiliation: Anatomical Institute, Kiel University, Otto-Hahn-Platz 8, 24118, Kiel, Germany.
authors.forename: Ralph
authors.initials: R
authors.lastname: Lucius
authors.affiliation: Biochemical Institute, Kiel University, Otto-Hahn-Platz 9, 24118, Kiel, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Becker-Pauly
mesh_terms: D000818:Animals; D003804:Dentin / Q000002:abnormalities*; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D007180:Incisor / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D008666:Metalloendopeptidases / Q000378:metabolism*; D051379:Mice; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D000072417:Protein Domains; D012795:Sialoglycoproteins / Q000737:chemistry / Q000378:metabolism*; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D008666:Metalloendopeptidases; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C053833:meprin A; C095663:meprin B
keywords: Collagen I; Dentin; Dentinogenesis imperfecta; Dentinsialophosphoprotein (DSPP); Meprinβ