title: Selenotranscriptomic Analyses Identify Signature Selenoproteins in Brain Regions in a Mouse Model of Parkinson's Disease.
abstract: Genes of selenoproteome have been increasingly implicated in various aspects of neurobiology and neurological disorders, but remain largely elusive in Parkinson's disease (PD). In this study, we investigated the selenotranscriptome (24 selenoproteins in total) in five brain regions (cerebellum, substantia nigra, cortex, pons and hippocampus) by real time qPCR in a two-phase manner using a mouse model of chronic PD. A wide range of changes in selenotranscriptome was observed in a manner depending on selenoproteins and brain regions. While Selv mRNA was not detectable and Dio1& 3 mRNA levels were not affected, 1, 11 and 9 selenoproteins displayed patterns of increase only, decrease only, and mixed response, respectively, in these brain regions of PD mice. In particular, the mRNA expression of Gpx1-4 showed only a decreased trend in the PD mouse brains. In substantia nigra, levels of 17 selenoprotein mRNAs were significantly decreased whereas no selenoprotein was up-regulated in the PD mice. In contrast, the majority of selenotranscriptome did not change and a few selenoprotein mRNAs that respond displayed a mixed pattern of up- and down-regulation in cerebellum, cortex, hippocampus, and/or pons of the PD mice. Gpx4, Sep15, Selm, Sepw1, and Sepp1 mRNAs were most abundant across all these five brain regions. Our results showed differential responses of selenoproteins in various brain regions of the PD mouse model, providing critical selenotranscriptomic profiling for future functional investigation of individual selenoprotein in PD etiology.
authors.affiliation: Department of Neurology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.|Department of Preventive Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Neurology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Yang-Lie
authors.initials: YL
authors.lastname: Ye
authors.affiliation: Department of Neurology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Preventive Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Sheng-Nan
authors.initials: SN
authors.lastname: Sun
authors.affiliation: Department of Preventive Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Preventive Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China.|Institute of Nutrition and Diseases, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Fan
authors.affiliation: Department of Preventive Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China.|Institute of Nutrition and Diseases, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Wu
authors.affiliation: Department of Neurology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Song-Fang
authors.initials: SF
authors.lastname: Chen
authors.affiliation: Department of Food Science, Nutrition and Health Promotion, Mississippi State University, Mississippi State, Mississippi, United States of America.
authors.forename: Wen-Hsing
authors.initials: WH
authors.lastname: Cheng
authors.affiliation: Department of Preventive Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, China.|Key Laboratory of Watershed Science and Health of Zhejiang Province, Wenzhou Medical University, Wenzhou, Zhejiang, China.|Institute of Nutrition and Diseases, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Zhu