title: Protective Effect of Cyanidin-3-O-Glucoside against Ultraviolet B Radiation-Induced Cell Damage in Human HaCaT Keratinocytes.
abstract: Ultraviolet radiation is the major environmental harmful factor that has emotional impact on human skin. The aim of the present study was to determine the mechanism of protection of cyanidin-3-O-glucoside against ultraviolet B (UVB)-induced damage to human HaCaT keratinocytes. Our results show that cyanidin-3-O-glucoside decreased the levels of intracellular reactive oxygen species generated by UVB treatment. Cyanidin-3-O-glucoside also decreased the UVB-augmented levels of the DNA damage indicators phospho-p53 and phospho-ATM/ATR. In addition, cyanidin-3-O-glucoside protected keratinocytes from UVB-induced injury by overturning the disruption of mitochondrial membrane potential and reversing apoptosis. The expression of anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) was attenuated in UVB-exposed cells but restored in UVB/cyanidin-3-O-glucoside-treated cells. Furthermore, expression of the proapoptotic proteins Bcl-2-associated X (Bax) and the key apoptosis executer cleaved caspase-3 were increased in UVB-irradiated cells and decreased in UVB/cyanidin-3-O-glucoside-treated cells. For these reasons, the results demonstrate that cyanidin-3-O-glucoside protects human keratinocytes against UVB-induced oxidative stress and apoptosis. Our study provides a theoretical basis for the use of cyanidin-3-O-glucoside in the fight against light damage.
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Jinan University, Jinan University Guangzhou, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Jinan University, Jinan University Guangzhou, China.
authors.forename: Yuetang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Jinan University, Jinan University Guangzhou, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Food Science and Engineering, Jinan University Guangzhou, China.
authors.forename: Tianfeng
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Jinan University, Jinan University Guangzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: He
authors.affiliation: Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology Guangzhou, China.
authors.forename: Jianxia
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Food Science and Engineering, Jinan University Guangzhou, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Jiao
authors.affiliation: Department of Food Science and Engineering, Jinan University Guangzhou, China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Food Science and Engineering, Jinan University Guangzhou, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Jinan University, Jinan University Guangzhou, China.
authors.forename: Liehua
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department of Food Science and Engineering, Jinan University Guangzhou, China.
authors.forename: Weibin
authors.initials: W
authors.lastname: Bai
keywords: Cyanidin-3-O-glucoside; HaCaT keratinocytes; apoptosis; reactive oxygen species; ultraviolet B radiation