title: Role of Long Noncoding RNA HOTAIR in the Growth and Apoptosis of Osteosarcoma Cell MG-63.
abstract: This study investigated the function of HOTAIR in the growth and apoptosis of OS MG-63 cell linein vitroand further clarified its mechanism. The expression levels of HOTAIR in OS MG-63 cell line and normal osteoblast hFOB1.19 cell line were determined by RT-PCR, respectively. The growth and apoptosis of MG-63 cellsin vitrowere investigated by MTT assay and flow cytometry assay after HOTAIR was knocked down with retroviral vector construction. And the expression levels of cell growth and apoptosis related factors TGF-β, p53, Bcl-2, and TNF-αwere determined to clarify the mechanism. We found that HOTAIR was highly expressed in osteosarcoma MG-63 cell line compared with normal osteoblast hFOB1.19 cell line. The proliferation rate was lower and the apoptosis rate was higher significantly in shHOTAIR MG-63 cells than those in EV MG-63 cells. TGF-βand Bcl-2 were downregulated significantly when HOTAIR was knocked down. p53 and TNF-αwere upregulated significantly when HOTAIR was knocked down. These results indicated that HOTAIR functioned as a carcinogenic lncRNA, which could promote the proliferation and inhibit the apoptosis of MG-63 cellsin vitro. HOTAIR could be a potential target for the treatment of osteosarcoma.
authors.affiliation: Department of Orthopedics, Yongchuan Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Orthopedics, Yongchuan Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Jing
authors.identifier: 0000-0001-5758-9774
authors.initials: J
authors.lastname: Min