title: The interplay between neuronal activity and actin dynamics mimic the setting of an LTD synaptic tag.
abstract: Persistent forms of plasticity, such as long-term depression (LTD), are dependent on the interplay between activity-dependent synaptic tags and the capture of plasticity-related proteins. We propose that the synaptic tag represents a structural alteration that turns synapses permissive to change. We found that modulation of actin dynamics has different roles in the induction and maintenance of LTD. Inhibition of either actin depolymerisation or polymerization blocks LTD induction whereas only the inhibition of actin depolymerisation blocks LTD maintenance. Interestingly, we found that actin depolymerisation and CaMKII activation are involved in LTD synaptic-tagging and capture. Moreover, inhibition of actin polymerisation mimics the setting of a synaptic tag, in an activity-dependent manner, allowing the expression of LTD in non-stimulated synapses. Suspending synaptic activation also restricts the time window of synaptic capture, which can be restored by inhibiting actin polymerization. Our results support our hypothesis that modulation of the actin cytoskeleton provides an input-specific signal for synaptic protein capture.
authors.affiliation: Cellular and Systems Neurobiology, Instituto Gulbenkian de Ciência, Rua da Quinta Grande, 6, 2780-156 Oeiras Portugal.
authors.forename: Eszter C
authors.initials: EC
authors.lastname: Szabó
authors.affiliation: Cellular and Systems Neurobiology, Instituto Gulbenkian de Ciência, Rua da Quinta Grande, 6, 2780-156 Oeiras Portugal.
authors.forename: Rita
authors.initials: R
authors.lastname: Manguinhas
authors.affiliation: Cellular and Systems Neurobiology, Instituto Gulbenkian de Ciência, Rua da Quinta Grande, 6, 2780-156 Oeiras Portugal.
authors.forename: Rosalina
authors.initials: R
authors.lastname: Fonseca
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D055724:Electrophysiological Phenomena; D036881:Long-Term Synaptic Depression; D008297:Male; D009474:Neurons / Q000378:metabolism*; D014176:Protein Biosynthesis; D055503:Protein Multimerization; D051381:Rats; D013569:Synapses / Q000378:metabolism*; D009435:Synaptic Transmission
chemical_list: D000199:Actins; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2