title: KH-type splicing regulatory protein (KHSRP) contributes to tumorigenesis by promoting miR-26a maturation in small cell lung cancer.
abstract: KH-type splicing regulatory protein (KHSRP) is a multifunctional RNA-binding protein that has a role in tumorigenesis of small cell lung cancer. The KHSRP protein level was shown to be significantly increased in small cell lung cancer (SCLC) tumor tissues compared with normal lung tissues by immunohistochemical staining. Moreover, KHSRP protein levels were strongly associated with T stage in patients with SCLC. Using in vitro assays, we found that knockdown of the KHSRP gene inhibited cell proliferation and increased cell apoptosis but had no effect on cell migration and invasion. We also showed that down-regulation of the KHSRP gene suppressed tumor growth in vivo. Further analyses indicated that KHSRP was involved in miR-26a maturation and inhibited the expression of PTEN in SCLC cells. Taken together, these findings suggested that KHSRP plays an important role in SCLC tumorigenesis and could be a potential novel therapeutic target for SCLC treatment.
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, 510282, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Tong
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, 510282, China.
authors.forename: Yingshan
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, 510282, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wei
authors.affiliation: Department of Pathology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Linlang
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou, China.
authors.forename: Haihong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, 510282, China. duarion@126.com.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Oncology, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, 510282, China. blacktiger@139.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D055752:Small Cell Lung Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D016601:RNA-Binding Proteins; D015534:Trans-Activators; C105789:KHSRP protein, human; C528163:MIRN26 microRNA, human
keywords: KH-type splicing regulatory protein (KHSRP); Phosphatase and tensin homolog deleted on chromosome ten (PTEN); Small cell lung cancer (SCLC); Tumorigenesis; mir-26a