title: A nuclease-assisted label-free aptasensor for fluorescence turn-on detection of ATP based on the in situ formation of copper nanoparticles.
abstract: Owing to their promising advantages in biochemical analysis, aptamer-based sensing systems for the fluorescence detection of important biomolecules are being extensively investigated. Herein, we propose a turn-on fluorescent aptasensor for label-free detection of adenosine triphosphate (ATP) by utilizing the in situ formation of copper nanoparticles (CuNPs) and the specific digestion capability of exonuclease I (Exo I). In this assay, the addition of ATP can effectively hinder the digestion of aptamer-derived oligonucleotides due to the G-quadruplex structure. Accordingly, the remaining poly thymine at 5'-terminus of substrate DNA can serve as an efficient template for red-emitting fluorescent CuNPs with a Mega-Stokes shifting in buffered solution, which can be used to evaluate the concentration of ATP. This method is cost-effective and facile, because it avoids the use of traditional dye-labeled DNA strands and complex operation steps. Under optimized conditions, this method achieves a selective response for ATP with a detection limit of 93nM, and exhibits a good detection performance in biological samples.
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, China; State Key Laboratory of Petroleum Pollution Control, Beijing 102206, China; CNPC Research Institute of Safety and Environment Technology, Beijing 102206, China.
authors.forename: Quanwei
authors.initials: Q
authors.lastname: Song
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Wang
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Petroleum Pollution Control, Beijing 102206, China; CNPC Research Institute of Safety and Environment Technology, Beijing 102206, China.
authors.forename: Hongkun
authors.initials: H
authors.lastname: Chen
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, China.
authors.forename: Zoumengke
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, China.
authors.forename: Na
authors.initials: N
authors.lastname: Na
authors.affiliation: Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, China. Electronic address: jinoyang@bnu.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Ouyang
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide; D003470:Culture Media; D005456:Fluorescent Dyes; D003300:Copper; D000255:Adenosine Triphosphate
keywords: Adenosine triphosphate; Aptamer; Copper nanoparticles; Fluorescence detection; Lable-free; Turn-on