title: Effect of Blood Component Coatings of Enosseal Implants on Proliferation and Synthetic Activity of Human Osteoblasts and Cytokine Production of Peripheral Blood Mononuclear Cells.
abstract: The study monitored in vitro early response of connective tissue cells and immunocompetent cells to enosseal implant materials coated by different blood components (serum, activated plasma, and plasma/platelets) to evaluate human osteoblast proliferation and synthetic activity and inflammatory response presented as a cytokine profile of peripheral blood mononuclear cells (PBMCs) under conditions imitating the situation upon implantation. The cells were cultivated on coated Ti-plasma-sprayed (Ti-PS), Ti-etched (Ti-Etch), Ti-hydroxyapatite (Ti-HA), and ZrO2 surfaces. The plasma/platelets coating supported osteoblast proliferation only on osteoconductive Ti-HA and Ti-Etch whereas activated plasma enhanced proliferation on all surfaces. Differentiation (BAP) and IL-8 production remained unchanged or decreased irrespective of the coating and surface; only the serum and plasma/platelets-coated ZrO2 exhibited higher BAP and IL-8 expression. RANKL production increased on serum and activated plasma coatings. PBMCs produced especially cytokines playing role in inflammatory phase of wound healing, that is, IL-6, GRO-α, GRO, ENA-78, IL-8, GM-CSF, EGF, and MCP-1. Cytokine profiles were comparable for all tested surfaces; only ENA-78, IL-8, GM-CSF, and MCP-1 expression depended on materials and coatings. The activated plasma coating led to uniformed surfaces and represented a favorable treatment especially for bioinert Ti-PS and ZrO2 whereas all coatings had no distinctive effect on bioactive Ti-HA and Ti-Etch.
authors.affiliation: School of Dental Medicine, General University Hospital in Prague, First Faculty of Medicine, Charles University in Prague, Karlovo Namesti 32, 121 11 Prague, Czech Republic.
authors.forename: Lucie
authors.identifier: 0000-0003-0846-5104
authors.initials: L
authors.lastname: Himmlova
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovskeho Namesti 2, 162 06 Prague, Czech Republic.
authors.forename: Dana
authors.identifier: 0000-0002-4759-2360
authors.initials: D
authors.lastname: Kubies
authors.affiliation: Rheumatological Institute, Na Slupi 4, 128 50 Prague, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Hulejova
authors.affiliation: School of Dental Medicine, General University Hospital in Prague, First Faculty of Medicine, Charles University in Prague, Karlovo Namesti 32, 121 11 Prague, Czech Republic.
authors.forename: Jirina
authors.initials: J
authors.lastname: Bartova
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovskeho Namesti 2, 162 06 Prague, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Riedel
authors.affiliation: The Institute of Hematology and Blood Transfusion, U Nemocnice 2094/1, 128 20 Prague, Czech Republic.
authors.forename: Jana
authors.identifier: 0000-0002-5964-0526
authors.initials: J
authors.lastname: Stikarova
authors.affiliation: The Institute of Hematology and Blood Transfusion, U Nemocnice 2094/1, 128 20 Prague, Czech Republic.
authors.forename: Jiri
authors.identifier: 0000-0003-4305-9718
authors.initials: J
authors.lastname: Suttnar
authors.affiliation: Rheumatological Institute, Na Slupi 4, 128 50 Prague, Czech Republic.
authors.forename: Vlasta
authors.initials: V
authors.lastname: Pesakova
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D018932:Chemokine CCL2 / Q000378:metabolism; D054360:Chemokine CXCL1 / Q000378:metabolism; D054365:Chemokine CXCL5 / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000009:adverse effects* / Q000737:chemistry*; D016207:Cytokines / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D014025:Titanium / Q000009:adverse effects / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects
chemical_list: C475829:CXCL1 protein, human; C486263:CXCL5 protein, human; D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D054365:Chemokine CXCL5; D020099:Coated Materials, Biocompatible; D016207:Cytokines; D015850:Interleukin-6; D016209:Interleukin-8; D004815:Epidermal Growth Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D014025:Titanium