title: Smoking and red blood cell phospholipid membrane fatty acids.
abstract: Smoking is associated with lower n-3 long chain polyunsaturated fatty acids (LCPUFA) concentrations; however, limited studies have accounted for dietary PUFA intake or whether tobacco dose or smoking duration influences this association. We measured red blood cell phospholipid (RBC) membrane concentrations of fatty acids in 126 current smokers, 311 former smokers, and 461 never smokers using gas liquid chromatography and tandem mass spectrometry. Smokers had lower RBC membrane percentages of total n-3 LCPUFAs compared to former smokers or never smokers (median percent: 5.46, [interquartile range (IQR) 4.52, 6.28] versus 6.39; [IQR: 5.18, 7.85] versus 6.59; [IQR 5.34, 8.01]) (p<0.001) and this association remained after adjusting for dietary PUFA intake. Duration of smoking and cigarettes per day were not associated with RBC membrane n-3 LCPUFA differences. Smoking is associated with lower n-3 LCPUFA RBC membrane percentages and this association was not influenced by diet or smoking dose or duration.
authors.affiliation: Division of General Medicine and Public Health, Vanderbilt University Medicine Center, Nashville, TN, United States; GRECC, Department of Veterans Affairs, Tennessee Valley Healthcare System, Nashville, TN, United States; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, United States. Electronic address: harvey.j.murff@vanderbilt.edu.
authors.forename: H J
authors.initials: HJ
authors.lastname: Murff
authors.affiliation: Division of General Medicine and Public Health, Vanderbilt University Medicine Center, Nashville, TN, United States; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, United States.
authors.forename: H A
authors.initials: HA
authors.lastname: Tindle
authors.affiliation: GRECC, Department of Veterans Affairs, Tennessee Valley Healthcare System, Nashville, TN, United States; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, United States; Division of Epidemiology, Vanderbilt University Medical Center, United States.
authors.forename: M J
authors.initials: MJ
authors.lastname: Shrubsole
authors.affiliation: Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, United States; Division of Epidemiology, Vanderbilt University Medical Center, United States.
authors.forename: Q
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Division of Gastroenterology, Hepatology and Nutrition, Vanderbilt University Medical Center, United States; Department of Health Policy, Vanderbilt University Medical Center, United States.
authors.forename: W
authors.initials: W
authors.lastname: Smalley
authors.affiliation: Division of Clinical Pharmacology, Vanderbilt University Medical Center, United States.
authors.forename: G L
authors.initials: GL
authors.lastname: Milne
authors.affiliation: Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, United States.
authors.forename: L L
authors.initials: LL
authors.lastname: Swift
authors.affiliation: Division of Gastroenterology, Hepatology and Nutrition, Vanderbilt University Medical Center, United States.
authors.forename: R M
authors.initials: RM
authors.lastname: Ness
authors.affiliation: GRECC, Department of Veterans Affairs, Tennessee Valley Healthcare System, Nashville, TN, United States; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, United States; Division of Epidemiology, Vanderbilt University Medical Center, United States.
authors.forename: W
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000328:Adult; D000368:Aged; D004910:Erythrocyte Membrane / Q000737:chemistry*; D004912:Erythrocytes / Q000737:chemistry; D005227:Fatty Acids / Q000097:blood*; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood*; D012907:Smoking / Q000009:adverse effects / Q000097:blood*
chemical_list: D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D010743:Phospholipids
keywords: Fatty acid, unsaturated; Oxidative stress; Tobacco use