title: Rescue of avian leukosis subgroup-J-associated acutely transforming viruses carrying different lengths of the v-fps oncogene and analysis of their tumorigenicity.
abstract: In our previous study, six subgroup J strains of avian leukosis virus (ALV-J)-associated acutely transforming viruses carrying different lengths of the v-fps oncogene, designated as Fu-J and Fu-J1-5, were isolated and characterized from fibrosarcomas in ALV-J-infected chickens. In the present study, the oncogenic potential of Fu-J and Fu-J1-5 was investigated using a reverse genetics technique. Six replication-defective viruses, named rFu-J and rFu-J1-5, were rescued with the replication-competent rescued ALV-J strain rSDAU1005 as a helper virus by co-transfection of chicken embryo fibroblast monolayers with infectious clone plasmids. Experimental bird studies were performed, demonstrating that only the rescued rFu-J virus carrying the complete v-fps oncogene with rSDAU1005 as the helper virus could induce acute fibrosarcoma after inoculation in specific-pathogen-free (SPF) chickens. These results provide direct evidence that the replication-defective acutely transforming Fu-J virus, with the complete v-fps oncogene, was associated with acute fibrosarcoma in chickens infected with ALV-J in the field, as reported previously.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Tai'an, Shandong, 271018, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Tai'an, Shandong, 271018, China.
authors.forename: Lichun
authors.initials: L
authors.lastname: Fang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Tai'an, Shandong, 271018, China.
authors.forename: Jianliang
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Tai'an, Shandong, 271018, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Tai'an, Shandong, 271018, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Cui
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Tai'an, Shandong, 271018, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Tai'an, Shandong, 271018, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Chang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Tai'an, Shandong, 271018, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Tai'an, Shandong, 271018, China. zzcui@sdau.edu.cn.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Cui
mesh_terms: D032761:Animal Experimentation; D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D015197:Carcinogenicity Tests; D002645:Chickens; D005354:Fibrosarcoma / Q000662:veterinary* / Q000821:virology; D006378:Helper Viruses; D015513:Oncogene Proteins / Q000235:genetics*; D011201:Poultry Diseases / Q000473:pathology* / Q000821:virology*; D013047:Specific Pathogen-Free Organisms; D014779:Virus Replication
chemical_list: D015513:Oncogene Proteins