title: Systems biology-opportunities and challenges: the application of proteomics to study the cardiovascular extracellular matrix.
abstract: Systems biology approaches including proteomics are becoming more widely used in cardiovascular research. In this review article, we focus on the application of proteomics to the cardiac extracellular matrix (ECM). ECM remodelling is a hallmark of many cardiovascular diseases. Proteomic techniques using mass spectrometry (MS) provide a platform for the comprehensive analysis of ECM proteins without a priori assumptions. Proteomics overcomes various constraints inherent to conventional antibody detection. On the other hand, studies that use whole tissue lysates for proteomic analysis mask the identification of the less abundant ECM constituents. In this review, we first discuss decellularization-based methods that enrich for ECM proteins in cardiac tissue, and how targeted MS allows for accurate protein quantification. The second part of the review will focus on post-translational modifications including hydroxylation and glycosylation and on the release of matrix fragments with biological activity (matrikines), all of which can be interrogated by proteomic techniques.
authors.affiliation: King's British Heart Foundation Centre, King's College London, 125 Coldharbour Lane, London SE5 9NU, UK.
authors.forename: Javier
authors.initials: J
authors.lastname: Barallobre-Barreiro
authors.affiliation: King's British Heart Foundation Centre, King's College London, 125 Coldharbour Lane, London SE5 9NU, UK.
authors.forename: Marc
authors.initials: M
authors.lastname: Lynch
authors.affiliation: King's British Heart Foundation Centre, King's College London, 125 Coldharbour Lane, London SE5 9NU, UK.
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Yin
authors.affiliation: King's British Heart Foundation Centre, King's College London, 125 Coldharbour Lane, London SE5 9NU, UK manuel.mayr@kcl.ac.uk.
authors.forename: Manuel
authors.initials: M
authors.lastname: Mayr
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000378:metabolism* / Q000503:physiopathology; D002319:Cardiovascular System / Q000378:metabolism* / Q000503:physiopathology; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D011499:Protein Processing, Post-Translational; D055550:Protein Stability; D059748:Proteolysis; D040901:Proteomics / Q000379:methods*; D049490:Systems Biology; D020257:Ventricular Remodeling
chemical_list: D015415:Biomarkers
keywords: Cardiovascular diseases; Extracellular matrix; Post-translational modifications; Proteomics; Systems biology