title: A virus resonance light scattering sensor based on mussel-inspired molecularly imprinted polymers for high sensitive and high selective detection of Hepatitis A Virus.
abstract: We described a novel resonance light scattering (RLS) sensor for the specific recognition of trace quantities of Hepatitis A Virus (HAV); the sensor was based on a mussel-inspired hepatitis molecularly imprinted polymer. As a recognition element, polydopamine (PDA)-coated totivirus-imprinted polymer was introduced on the surface of SiO2nanoparticles (virus-imprinted SiO2@PDA NPs) using an efficient one-step synthesis method. The target virus was selectively captured by the imprinted polymer films, thereby increasing the RLS intensity. A simple fluorescence spectrophotometer was employed to measure the changes in the intensity. The enhanced RLS intensity (∆IRLS) was proportional to the concentration of HAV in the range of 0.04-6.0nmol∙L-1, with a low limit of detection of 8.6pmol∙L-1. The selectivity study confirmed that the resultant HAV-imprinted SiO2@PDA NPs possessed high selectivity for HAV. The sensor was successfully applied for the direct detection of additional HAV from a 20,000-fold dilution of human serum. The proposed strategy is simple, eco-friendly, highly selective, and sensitive.
authors.affiliation: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Gong
authors.affiliation: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Chen
authors.affiliation: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China. Electronic address: cai_mao3@hotmail.com.
authors.forename: Changqun
authors.initials: C
authors.lastname: Cai
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D049872:Bivalvia / Q000737:chemistry; D000067493:Dynamic Light Scattering / Q000379:methods; D006506:Hepatitis A / Q000097:blood* / Q000175:diagnosis / Q000821:virology; D030041:Hepatitis A virus / Q000302:isolation & purification*; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D057230:Limit of Detection; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D007211:Indoles; D011108:Polymers; C568283:polydopamine; D012822:Silicon Dioxide
keywords: Hepatitis A Virus (HAV); Resonance Light Scattering (RLS) sensor; Virus-molecularly imprinted polymer (virus-MIPs)