title: Enduring disturbances in regional cerebral blood flow and brain oxygenation at 24 h after asphyxial cardiac arrest in developing rats.
abstract: Disturbances in cerebral blood flow (CBF) and brain oxygenation (PbO2) are present early after pediatric cardiac arrest (CA). CBF-targeted therapies improved neurological outcome in our CA model. To assess the therapeutic window for CBF- and PbO2-targeted therapies, we propose to determine if CBF and PbO2disturbances persist at 24 h after experimental pediatric CA.
  
  
  
  Regional CBF and PbO2were measured at 24 h after asphyxial CA in immature rats (n = 26, 6-8/group) using arterial spin label MRI and tissue electrodes, respectively.
  
  
  
  In all regions but the thalamus, CBF recovered to sham values by 24 h; thalamic CBF was >32% higher after CA vs. sham. PbO2values at 24 h after CA in the cortex and thalamus were similar to shams in rats who received supplemental oxygen, however, on room air, cortical PbO2was lower after CA vs. shams.
  
  
  
  CBF remains increased in the thalamus at 24 h after CA and PbO2is decreased to hypoxic levels in cortex at 24 h after CA in rats who do not receive supplemental oxygen. Given the enduring disturbances in this model and the lack of routine CBF or PbO2monitoring in patients, our data suggest the need for clinical correlation.
authors.affiliation: Animal Imaging Center, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Lesley M
authors.initials: LM
authors.lastname: Foley
authors.affiliation: Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.|Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.|Safar Center for Resuscitation Research, Pittsburgh, Pennsylvania.
authors.forename: Robert S B
authors.initials: RS
authors.lastname: Clark
authors.affiliation: Animal Imaging Center, University of Pittsburgh, Pittsburgh, Pennsylvania.|Department of Radiology, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Alberto L
authors.initials: AL
authors.lastname: Vazquez
authors.affiliation: Animal Imaging Center, University of Pittsburgh, Pittsburgh, Pennsylvania.|Department of Neurobiology, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: T Kevin
authors.initials: TK
authors.lastname: Hitchens
authors.affiliation: Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.|Safar Center for Resuscitation Research, Pittsburgh, Pennsylvania.
authors.forename: Henry
authors.initials: H
authors.lastname: Alexander
authors.affiliation: Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania.
authors.forename: Chien
authors.initials: C
authors.lastname: Ho
authors.affiliation: Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.|Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.|Safar Center for Resuscitation Research, Pittsburgh, Pennsylvania.|Department of Anesthesiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.|Department of Bioengineering, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.|Department of Clinical and Translational Science, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Kochanek
authors.affiliation: Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.|Safar Center for Resuscitation Research, Pittsburgh, Pennsylvania.
authors.forename: Mioara D
authors.initials: MD
authors.lastname: Manole
mesh_terms: D000818:Animals; D001237:Asphyxia / Q000503:physiopathology* / Q000628:therapy; D001921:Brain / Q000503:physiopathology*; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004195:Disease Models, Animal; D006323:Heart Arrest / Q000503:physiopathology* / Q000628:therapy; D000860:Hypoxia / Q000503:physiopathology / Q000628:therapy; D008297:Male; D010100:Oxygen / Q000627:therapeutic use; D010101:Oxygen Consumption / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013788:Thalamus / Q000098:blood supply / Q000378:metabolism
chemical_list: D010100:Oxygen