title: Discovery, screening and evaluation of a plasma biomarker panel for subjects with psychological suboptimal health state using (1)H-NMR-based metabolomics profiles.
abstract: Individuals in the state of psychological suboptimal health keep increasing, only scales and questionnaires were used to diagnose in clinic under current conditions, and symptoms of high reliability and accuracy are destitute. Therefore, the noninvasive and precise laboratory diagnostic methods are needed. This study aimed to develop an objective method through screen potential biomarkers or a biomarker panel to facilitate the diagnosis in clinic using plasma metabolomics. Profiles were based on H-nuclear magnetic resonance ((1)H-NMR) metabolomics techniques combing with multivariate statistical analysis. Furthermore, methods of correlation analysis with Metaboanalyst 3.0 for selecting a biomarker panel, traditional Chinese medicine (TCM) drug intervention for validating the close relations between the biomarker panel and the state and the receiver operating characteristic curves (ROC curves) analysis for evaluation of clinical diagnosis ability were carried out. 9 endogenous metabolites containing trimethylamine oxide (TMAO), glutamine, N-acetyl-glycoproteins, citrate, tyrosine, phenylalanine, isoleucine, valine and glucose were identified and considered as potential biomarkers. Then a biomarker panel consisting of phenylalanine, glutamine, tyrosine, citrate, N-acetyl-glycoproteins and TMAO was selected, which exhibited the highest area under the curve (AUC = 0.971). This study provided critical insight into the pathological mechanism of psychological suboptimal health and would supply a novel and valuable diagnostic method.
authors.affiliation: Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, P. R. China.
authors.forename: Jun-Sheng
authors.initials: JS
authors.lastname: Tian
authors.affiliation: Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, P. R. China.|College of Chemistry and Chemical Engineering of Shanxi University, Taiyuan 030006, P. R. China.
authors.forename: Xiao-Tao
authors.initials: XT
authors.lastname: Xia
authors.affiliation: Department of traditional Chinese medicine, First Hospital of Shanxi Medical University, Taiyuan 030001, P. R. China.
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Wu
authors.affiliation: Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Physical Education Departments of Shanxi University, Taiyuan 030006, P. R. China.
authors.forename: Huan
authors.initials: H
authors.lastname: Xiang
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100050, P. R. China.
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Du
authors.affiliation: Department of Chemistry of University of Louisville, Louisville, KY40292 United States.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, P. R. China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Qin
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage; D005260:Female; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional / Q000379:methods; D001523:Mental Disorders / Q000097:blood* / Q000188:drug therapy; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal