title: Interplay of nuclear receptors (ER, PR, and GR) and their steroid hormones in MCF-7 cells.
abstract: Steroid hormones and their nuclear receptors play a major role in the development and progression of breast cancer. MCF-7 cells are triple-positive breast cancer cells expressing estrogen receptor (ER), progesterone receptor (PR), and glucocorticoid receptor (GR). However, interaction and their role in expression pattern of activator protein (AP-1) transcription factors (TFs) are not completely understood. Hence, in our study, MCF-7 cells were used as an in vitro model system to study the interplay between the receptors and hormones. MCF-7 cells were treated with estradiol-17β (E2), progesterone (P4), and dexamethasone (Dex), alone or in combination, to study the proliferation of cells and expression of AP-1 genes. MTT assay results show that E2 or P4 induced the cell proliferation by more than 35 %, and Dex decreased the proliferation by 26 %. E2 and P4 are found to increase ERα by more than twofold and c-Jun, c-Fos, and Fra-1 AP-1 TFs by more than 1.7-fold, while Dex shows opposite effect of E2- or P4-induced effect as well as effect on the expression of nuclear receptors and AP-1 factors. E2 antagonist Fulvestrant (ICI 182,780) found to reduce proliferation and E2-induced expression of AP1-TFs, while P4 or Dex antagonist Mifepristone (RU486) is found to block GR-mediated expression of NRs and AP-1 mRNAs. Results suggest that E2 and P4 act synergistically, and Dex acts as an antagonist of E2 and P4.
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru, Karnataka, 560 056, India.
authors.forename: Shubha M
authors.initials: SM
authors.lastname: Hegde
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru, Karnataka, 560 056, India.
authors.forename: M Naveen
authors.initials: MN
authors.lastname: Kumar
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru, Karnataka, 560 056, India.
authors.forename: K
authors.initials: K
authors.lastname: Kavya
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru, Karnataka, 560 056, India.
authors.forename: K M Kiran
authors.initials: KM
authors.lastname: Kumar
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru, Karnataka, 560 056, India.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Nagesh
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru, Karnataka, 560 056, India.
authors.forename: Rajeshwari H
authors.initials: RH
authors.lastname: Patil
authors.affiliation: Department of Bioinformatics and Biotechnology, Karnataka State Women's University, Jnanashakthi Campus, Vijayapura, Karnataka, 586 108, India.
authors.forename: R L
authors.initials: RL
authors.lastname: Babu
authors.affiliation: Department of Biology and Center for Biotechnology and Biomedical Sciences, Norfolk State University, Norfolk, VA, USA.
authors.forename: Govindarajan T
authors.initials: GT
authors.lastname: Ramesh
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru, Karnataka, 560 056, India. sharma@bub.ernet.in.
authors.forename: S Chidananda
authors.initials: SC
authors.lastname: Sharma
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D003907:Dexamethasone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D009363:Neoplasm Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D011965:Receptors, Glucocorticoid / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000819:agonists / Q000235:genetics / Q000378:metabolism*
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D009363:Neoplasm Proteins; D011965:Receptors, Glucocorticoid; D011980:Receptors, Progesterone; D011374:Progesterone; D004958:Estradiol; D003907:Dexamethasone
keywords: AP-1; MCF-7; Nuclear receptors; RT-PCR; Steroid hormones