title: [Relationship between R236C site in exon 7 of SP-B gene and respiratory distress syndrome in Han newborns in western Inner Mongolia].
abstract: OBJECTIVE
  To detect and analyze the genetic variation in exon 7 of lung surfactant protein B (SP-B), and to investigate the relationship between the genetic variation and the incidence of neonatal respiratory distress syndrome (NRDS) in Han populations in western Inner Mongolia.
  
  
  METHODS
  In the case-control study, 47 Han infants with NRDS were assigned to case group. All the 47 patients had the last three generations of their ancestors reside in western Inner Mongolia. Forty-seven Han newborns without NRDS were assigned to control group. PCR-based gene analysis was used to determine the mutation in exon 7 of SP-B gene and genotype and allele frequencies of the R236C site in exon 7 of SP-B gene.
  
  
  RESULTS
  In Han newborns in western Inner Mongolia, there was no mutation in exon 7 of SP-B gene; two genotypes, CC and CT, were identified in the R236C site in exon 7 of SP-B gene. No TT genotype was found in the two groups. There were no significant differences in the genotype frequency of CC or CT as well as the allele frequency of C or T between the case and control groups (CC: 72% vs 85%, P>0.05; CT: 28% vs 15%, P>0.05; C: 85% vs 93%, P>0.05; T: 15% vs 7%, P>0.05).
  
  
  CONCLUSIONS
  There is no mutation in exon 7 of SP-B gene in Han infants with NRDS in western Inner Mongolia. There is no significant association between the gene polymorphism of the R236C site in exon 7 of SP-B gene and the incidence of NRDS in Han populations in that region.
authors.affiliation: Department of Neonatology, Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010059, China. meihuayani@sina.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Mei
authors.forename: Chun-Zhi
authors.initials: CZ
authors.lastname: Liu
authors.forename: Ya-Yu
authors.initials: YY
authors.lastname: Zhang
authors.forename: Chun-Li
authors.initials: CL
authors.lastname: Liu
authors.forename: Dan
authors.initials: D
authors.lastname: Song
authors.forename: Yu-Heng
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D016022:Case-Control Studies; D002681:China; D005091:Exons; D005260:Female; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D011110:Polymorphism, Genetic; D037701:Pulmonary Surfactant-Associated Protein B / Q000235:genetics*; D012127:Respiratory Distress Syndrome, Newborn / Q000235:genetics*
chemical_list: D037701:Pulmonary Surfactant-Associated Protein B