title: Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology.
abstract: The tiger shark (Galeocerdo cuvier) has a global distribution in tropical and warm temperate seas, and it is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the International Union for Conservation of Nature (IUCN) Red List. In this study, we identified nine microsatellite loci through next generation sequencing (454 pyrosequencing) using 29 samples from the western Atlantic. The genetic diversity of these loci were assessed and revealed a total of 48 alleles ranging from 3 to 7 alleles per locus (average of 5.3 alleles). Cross-species amplification was successful at most loci for other species such as Carcharhinus longimanus, C. acronotus and Alopias superciliosus. Given the potential applicability of genetic markers for biological conservation, these data may contribute to the population assessment of this and other species of sharks worldwide.
authors.affiliation: Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista , Botucatu, São Paulo , Brazil.
authors.forename: Natália J
authors.initials: NJ
authors.lastname: Mendes
authors.affiliation: Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista , Botucatu, São Paulo , Brazil.
authors.forename: Vanessa P
authors.initials: VP
authors.lastname: Cruz
authors.affiliation: Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista , Botucatu, São Paulo , Brazil.
authors.forename: Fernando Y
authors.initials: FY
authors.lastname: Ashikaga
authors.affiliation: Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil; Laboratório de Genética Pesqueira e Conservação, Institute of Marine Sciences, Universidade Federal de São Paulo, Santos, São Paulo, Brazil.
authors.forename: Sâmia M
authors.initials: SM
authors.lastname: Camargo
authors.affiliation: Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista , Botucatu, São Paulo , Brazil.
authors.forename: Claudio
authors.initials: C
authors.lastname: Oliveira
authors.affiliation: Lake Nona Campus, Valencia College , Orlando, Florida , United States.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Piercy
authors.affiliation: Florida Museum of Natural History, Florida Program for Shark Research, University of Florida , Gainesville, Florida , United States.
authors.forename: George H
authors.initials: GH
authors.lastname: Burgess
authors.affiliation: IPMA, Portuguese Institute for the Ocean and Atmosphere , Algarve , Portugal.
authors.forename: Rui
authors.initials: R
authors.lastname: Coelho
authors.affiliation: IPMA, Portuguese Institute for the Ocean and Atmosphere , Algarve , Portugal.
authors.forename: Miguel N
authors.initials: MN
authors.lastname: Santos
authors.affiliation: Laboratório de Genética Pesqueira e Conservação, Institute of Marine Sciences, Universidade Federal de São Paulo , Santos, São Paulo , Brazil.
authors.forename: Fernando F
authors.initials: FF
authors.lastname: Mendonça
authors.affiliation: Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista , Botucatu, São Paulo , Brazil.
authors.forename: Fausto
authors.initials: F
authors.lastname: Foresti
keywords: Galeocerdo cuvier; High-throughput sequencing; Microsatellites; Population structure; Shark