title: Plasmablasts During Acute Dengue Infection Represent a Small Subset of a Broader Virus-specific Memory B Cell Pool.
abstract: Dengue is endemic in tropical countries worldwide and the four dengue virus serotypes often co-circulate. Infection with one serotype results in high titers of cross-reactive antibodies produced by plasmablasts, protecting temporarily against all serotypes, but impairing protective immunity in subsequent infections. To understand the development of these plasmablasts, we analyzed virus-specific B cell properties in patients during acute disease and at convalescence. Plasmablasts were unrelated to classical memory cells expanding in the blood during early recovery. We propose that only a small subset of memory B cells is activated as plasmablasts during repeat infection and that plasmablast responses are not representative of the memory B cell repertoire after dengue infection.
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Ramapraba
authors.initials: R
authors.lastname: Appanna
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Kg
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Mei Hui
authors.initials: MH
authors.lastname: Xu
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Ying-Xiu
authors.initials: YX
authors.lastname: Toh
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Sumathy
authors.initials: S
authors.lastname: Velumani
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Daniel
authors.initials: D
authors.lastname: Carbajo
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Chia Yin
authors.initials: CY
authors.lastname: Lee
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Roland
authors.initials: R
authors.lastname: Zuest
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Thavamalar
authors.initials: T
authors.lastname: Balakrishnan
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Weili
authors.initials: W
authors.lastname: Xu
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Bernett
authors.initials: B
authors.lastname: Lee
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Michael
authors.initials: M
authors.lastname: Poidinger
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Francesca
authors.initials: F
authors.lastname: Zolezzi
authors.affiliation: Communicable Disease Centre, Institute of Infectious Disease and Epidemiology, Tan Tock Seng Hospital, Singapore; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Yee Sin
authors.initials: YS
authors.lastname: Leo
authors.affiliation: Communicable Disease Centre, Institute of Infectious Disease and Epidemiology, Tan Tock Seng Hospital, Singapore.
authors.forename: Tun Linn
authors.initials: TL
authors.lastname: Thein
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore.
authors.forename: Cheng-I
authors.initials: CI
authors.lastname: Wang
authors.affiliation: Singapore Immunology Network, A*STAR, Singapore. Electronic address: katja_fink@immunol.a-star.edu.sg.
authors.forename: Katja
authors.initials: K
authors.lastname: Fink
mesh_terms: D000208:Acute Disease; D000818:Animals; D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D060965:Clonal Evolution; D003429:Cross Reactions / Q000276:immunology; D003715:Dengue / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D003716:Dengue Virus / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007156:Immunologic Memory; D016130:Immunophenotyping; D009500:Neutralization Tests; D010641:Phenotype; D010950:Plasma Cells / Q000276:immunology* / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region
keywords: Antibodies; Dengue; Memory B cells; Plasmablasts