title: Seropositivity and identification of paramyosin for sparganosis in the Kangwon and Incheon provinces of the Republic of Korea.
abstract: Sparganosis is one of the top three tissue-dwelling heterologous helminthic diseases, along with cysticercosis and paragonimiasis, in Korea. Due to a lack of effective early diagnosis and treatment methods, this parasitic disease is regarded as a public health threat. This study evaluated reactivity, against sparganum extracts, of sera from inhabitants of Cheorwon-gun, Goseong-gun and Ongjin-gun in Korea. The sera from 836 subjects were subjected to enzyme-linked immunosorbent assay and immunoblot analysis. The sera from 18 (5.8%) and 15 (5.1%) inhabitants in Cheorwon-gun (n = 312) and Goseong-gun (n = 294), respectively, exhibited highly positive reactions to the sparganum antigen, whereas only two (0.9%) inhabitants in Ongjin-gun (n = 230) showed positivity. We sought antigenic proteins for serodiagnosis of positive sera by immunoproteomic approaches. Total sparganum lysates were separated by two-dimensional electrophoresis and then subjected to immunoblot analysis with mixed sparganosis-positive sera. We found seven antigenic spots and identified paramyosin as an antigenic protein by liquid chromatography-mass spectrometry. By two-dimensional (2D)-based mass analysis and immunoblotting against sparganosis-positive sera, paramyosin was identified as a candidate antigen for serodiagnosis of sparganosis.
authors.affiliation: Division of Malaria and Parasitic Diseases,Center for Immunology and Pathology, National Research Institute of Health, Centers for Disease Control and Prevention,Osong, 28159,Republic of Korea.
authors.forename: M-R
authors.initials: MR
authors.lastname: Lee
authors.affiliation: Division of Malaria and Parasitic Diseases,Center for Immunology and Pathology, National Research Institute of Health, Centers for Disease Control and Prevention,Osong, 28159,Republic of Korea.
authors.forename: J-W
authors.initials: JW
authors.lastname: Ju
authors.affiliation: Department of Parasitology and Tropical Medicine,Kyungpook National University School of Medicine,101 Dongin-2ga,Jong-gu,Daegu, 70-422,Republic of Korea.
authors.forename: H-W
authors.initials: HW
authors.lastname: Yang
authors.affiliation: Department of Parasitology,Inha University School of Medicine,Incheon 400-103,Republic of Korea.
authors.forename: T-S
authors.initials: TS
authors.lastname: Kim
authors.affiliation: Division of Malaria and Parasitic Diseases,Center for Immunology and Pathology, National Research Institute of Health, Centers for Disease Control and Prevention,Osong, 28159,Republic of Korea.
authors.forename: M-Y
authors.initials: MY
authors.lastname: Park
authors.affiliation: Division of Malaria and Parasitic Diseases,Center for Immunology and Pathology, National Research Institute of Health, Centers for Disease Control and Prevention,Osong, 28159,Republic of Korea.
authors.forename: S-H
authors.initials: SH
authors.lastname: Cho
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D015151:Immunoblotting; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis; D056910:Republic of Korea; D012698:Serologic Tests / Q000379:methods*; D013031:Sparganosis / Q000175:diagnosis*; D013032:Sparganum / Q000737:chemistry / Q000276:immunology*; D014335:Tropomyosin / Q000032:analysis / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D020543:Proteome; D014335:Tropomyosin