title: A Mouse Model of Fluorescent Protein-expressing Disseminated Peritoneal Lymphoma for Fluorescence-guided Surgery.
abstract: Fluorescence-guided surgery (FGS) of tumors is an area of intense development. Peritoneally-disseminated cancer, however, represents a difficult surgical challenge.
  
  
  
  To help meet this challenge, EL4 mouse T-cell lymphoma cells expressing red fluorescent protein (RFP) were injected intraperitoneally in nude mice.
  
  
  
  Two weeks after injection of EL4-RFP cells, established peritoneally-disseminated tumors were observed. FGS was performed using a hand-held portable fluorescence imaging system (Dino-Lite). FGS enabled detection of very small peritoneal disseminated tumors and completely resected them in contrast to bright-light which only partially detected the tumors.
  
  
  
  The present report indicates the feasibility of FGS of peritoneally-disseminated cancer.
authors.affiliation: Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: Gifu University Graduate School of Medicine, Gifu, Japan AntiCancer, Inc., San Diego, CA, U.S.A. Department of Surgery, University of California San Diego, San Diego, CA, U.S.A. all@anticancer.com asue@gifu-u.ac.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suetsugu
authors.affiliation: Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Hasegawa
authors.affiliation: Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Nakamura
authors.affiliation: Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Shibata
authors.affiliation: Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Aoki
authors.affiliation: Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kunisada
authors.affiliation: Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Tsurumi
authors.affiliation: Gifu University Graduate School of Medicine, Gifu, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Shimizu
authors.affiliation: Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.affiliation: AntiCancer, Inc., San Diego, CA, U.S.A. Department of Surgery, University of California San Diego, San Diego, CA, U.S.A. all@anticancer.com asue@gifu-u.ac.jp.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D008164:Luminescent Proteins / Q000378:metabolism; D008223:Lymphoma / Q000000981:diagnostic imaging* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D061848:Optical Imaging; D010534:Peritoneal Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D025321:Surgery, Computer-Assisted / Q000379:methods; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein
keywords: RFP; fluorescence guided surgery; imaging; malignant lymphoma; nude mice; peritoneal dissemination