title: An engineered Lactococcus lactis strain exerts significant immune responses through efficient expression and delivery of Helicobacter pylori Lpp20 antigen.
abstract: OBJECTIVE
  To produce and deliver Helicobacter pylori lipoprotein Lpp20 via using Lactococcus lactis with aim of developing an efficient way to use this protective antigen in vaccine formulation.
  
  
  RESULTS
  An engineered L. lactis strain carrying the lpp20 gene from H. pylori was constructed. The inducible expression of Lpp20 in L. lactis was detected as a 20 kDa intracellular protein by SDS-PAGE. Lpp20 constituted 10 % of the L. lactis cellular proteins. The expression product was highly immunoreactive, as demonstrated by western blot assays using mouse anti-H. pylori sera. Animal experimentation showed that oral vaccination with the engineered strain excited significantly elevated levels of serum Lpp20-specific IgG antibodies in BALB/c mice (P < 0.05).
  
  
  CONCLUSIONS
  This report presents the first efficient expression and delivery of whole Lpp20 protein to the immunization sites by using L. lactis, demonstrating an efficient utilization mode of Lpp20 in anti-H. pylori vaccination.
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.|Henan Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang, 453003, China.
authors.forename: Rongguang
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China. gcduan@zzu.edu.cn.|Henan Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang, 453003, China. gcduan@zzu.edu.cn.
authors.forename: Guangcai
authors.initials: G
authors.lastname: Duan
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.
authors.forename: Shuaiyin
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.
authors.forename: Qingtang
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Dadao, Zhengzhou, 450001, China.
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Xi
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology / Q000378:metabolism*; D016480:Helicobacter pylori / Q000276:immunology* / Q000378:metabolism; D013294:Lactococcus lactis / Q000276:immunology* / Q000378:metabolism*; D051379:Mice
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial
keywords: Helicobactor pylori; Humoral immune; Lactococcus lactis; Lpp20; Oral vaccination