title: Application of MoS2 modified screen-printed electrodes for highly sensitive detection of bovine serum albumin.
abstract: The present work reports the application of a new molybdenum disulphide (MoS2)-based electrochemical platform for highly sensitive quantitation of an iron-binding protein, bovine serum albumin (BSA). The gold screen-printed electrodes were modified with MoS2 nanoflakes, followed by bioconjugation with anti-BSA antibodies. Using the above bioelectrode, cyclic voltammetric analysis was carried out in the presence of a Fe(3+)/Fe(2+) redox probe which exhibited a linear response of peak current with varying concentrations of BSA up to 10 ng/mL (with a detection limit of 0.006 ng/mL). This study is novel in that it shows a considerable enhancement of signal during electrochemical sensing of a protein.
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30-C, Chandigarh, 160030, India; Academy of Scientific and Innovative Research, CSIR-CSIO, Sector 30-C, Chandigarh, 160030, India.
authors.forename: Manil
authors.initials: M
authors.lastname: Kukkar
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30-C, Chandigarh, 160030, India; UIET, Panjab University, Sector 14, Chandigarh, 160014, India.
authors.forename: Ashish
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, India.
authors.forename: Parveen
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Civil & Environmental Engineering, Hanyang University, 222 Wangsimni-Ro, Seoul 04763, Republic of Korea. Electronic address: kkim61@hanyang.ac.kr.
authors.forename: Ki-Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30-C, Chandigarh, 160030, India; Academy of Scientific and Innovative Research, CSIR-CSIO, Sector 30-C, Chandigarh, 160030, India. Electronic address: dr.akashdeep@csio.res.in.
authors.forename: Akash
authors.initials: A
authors.lastname: Deep
mesh_terms: D000818:Animals; D002417:Cattle; D004220:Disulfides / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D057230:Limit of Detection; D008982:Molybdenum / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D011327:Printing; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry
chemical_list: D004220:Disulfides; D012710:Serum Albumin, Bovine; D008982:Molybdenum; C082964:molybdenum disulfide
keywords: Bovine serum albumin; Cyclic voltammetry; Immunosensing; MoS(2)