title: Association between DNA methylation of SORL1 5'-flanking region and mild cognitive impairment in type 2 diabetes mellitus.
abstract: OBJECTIVE
  In the present study, we examined whether DNA methylation of the sortilin-related receptor 1 (SORL1) 5'-flanking region is associated with the manifestation and clinical presentation of mild cognitive impairment in type 2 diabetes mellitus (T2DM).
  
  
  METHODS
  Of 84 diabetic patients with mild cognitive impairment (MCI group), 78 diabetic patients without mild cognitive impairment (NMCI group) and 80 age-matched normal controls (NC group), the DNA methylation of the SORL1 5'-flanking region was completely analyzed. The SORL1 methylation ratios of the above three groups were compared statistically. Next, we investigated the correlation between the DNA methylation status and the clinical presentation of diabetes with or without cognitive impairment (MCI and NMCI groups).
  
  
  RESULTS
  The methylation ratio (86.9%) of MCI patients was significantly higher than that in the NMCI patients (35.9%, P<0.05) and in the NC group (11.3%, P<0.05). Moreover, the diabetic patients with methylation alleles had greater ages, longer diabetes duration, lower MOCA scores and higher plasma amyloid Aβ 1-42 levels than those with unmethylation alleles (P<0.05).
  
  
  CONCLUSIONS
  These results suggested that the DNA methylation of the SORL1 5'-flanking region may significantly influence the manifestation of mild cognitive impairment in T2DM, and might be associated with its neurocognitive presentation.
authors.affiliation: Endocrinology department of Affiliated Zhongshan Hospital of Dalian University, 6 JieFang Street, Zhongshan District, DaLian, 116001 Liaoning, PR China. Electronic address: someso@sina.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Endocrinology department of Affiliated Zhongshan Hospital of Dalian University, 6 JieFang Street, Zhongshan District, DaLian, 116001 Liaoning, PR China.
authors.forename: Wang
authors.initials: W
authors.lastname: Mingjiao
authors.affiliation: Endocrinology department of Affiliated Zhongshan Hospital of Dalian University, 6 JieFang Street, Zhongshan District, DaLian, 116001 Liaoning, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Yang
authors.affiliation: Endocrinology department of Affiliated Zhongshan Hospital of Dalian University, 6 JieFang Street, Zhongshan District, DaLian, 116001 Liaoning, PR China.
authors.forename: Miao
authors.initials: M
authors.lastname: Sui
authors.affiliation: Endocrinology department of Affiliated Zhongshan Hospital of Dalian University, 6 JieFang Street, Zhongshan District, DaLian, 116001 Liaoning, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Neurology department of Affiliated Zhongshan Hospital of Dalian University, DaLian, 116001 Liaoning, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liang
authors.affiliation: Neurology department of Affiliated Zhongshan Hospital of Dalian University, DaLian, 116001 Liaoning, PR China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Gu
authors.affiliation: Endocrinology department of Affiliated Zhongshan Hospital of Dalian University, 6 JieFang Street, Zhongshan District, DaLian, 116001 Liaoning, PR China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
mesh_terms: D024506:5' Flanking Region / Q000235:genetics*; D000368:Aged; D016022:Case-Control Studies; D003072:Cognition Disorders / Q000235:genetics; D060825:Cognitive Dysfunction / Q000150:complications / Q000235:genetics*; D019175:DNA Methylation; D048909:Diabetes Complications / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D026502:LDL-Receptor Related Proteins / Q000235:genetics*; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D026502:LDL-Receptor Related Proteins; D026901:Membrane Transport Proteins; C102582:SORL1 protein, human
keywords: Déficience cognitive légère; Methylation; Methylation-specific PCR; Mild cognitive impairment; Méthylation; Méthylation par PCR; SORL1