title: Modulatory Effects of Egg White Ovotransferrin-Derived Tripeptide IRW (Ile-Arg-Trp) on Vascular Smooth Muscle Cells against Angiotensin II Stimulation.
abstract: The renin angiotensin system (RAS) is a key mediator of blood pressure regulation. Angiotensin II (Ang II), the active component of RAS, is a potent vasoconstrictor that also causes abnormal proliferation, oxidative stress, and inflammation in vascular smooth muscle cells (VSMCs) that contribute to atherosclerotic changes. Egg white ovotransferrin-derived tripeptide IRW (Ile-Arg-Trp) was previously shown to exert antihypertensive effect by reducing Ang II synthesis as well as endothelial cell inflammation and endothelial dysfunction. However, the effects of IRW on VSMCs are still unclear. In the present study, we evaluated the antiproliferative, antioxidant, and anti-inflammatory effects of IRW on VSMCs in the presence of Ang II stimulation. It was found that IRW treatment could attenuate Ang II-stimulated proliferation, superoxide production, and inflammation in VSMCs. These beneficial effects appeared to involve modulation of the NF-κB pathway. These findings could further our understanding on the antihypertensive mechanism of IRW beyond vascular endothelium.
authors.affiliation: Department of Agricultural, Food & Nutritional Science, ‡Department of Obstetrics & Gynecology, §Department of Physiology, ∥Cardiovascular Research Centre, and ⊥Women and Children's Health Research Institute, University of Alberta , Edmonton, Alberta T6G 2P5, Canada.
authors.forename: Wang
authors.initials: W
authors.lastname: Liao
authors.affiliation: Department of Agricultural, Food & Nutritional Science, ‡Department of Obstetrics & Gynecology, §Department of Physiology, ∥Cardiovascular Research Centre, and ⊥Women and Children's Health Research Institute, University of Alberta , Edmonton, Alberta T6G 2P5, Canada.
authors.forename: Subhadeep
authors.initials: S
authors.lastname: Chakrabarti
authors.affiliation: Department of Agricultural, Food & Nutritional Science, ‡Department of Obstetrics & Gynecology, §Department of Physiology, ∥Cardiovascular Research Centre, and ⊥Women and Children's Health Research Institute, University of Alberta , Edmonton, Alberta T6G 2P5, Canada.
authors.forename: Sandra T
authors.initials: ST
authors.lastname: Davidge
authors.affiliation: Department of Agricultural, Food & Nutritional Science, ‡Department of Obstetrics & Gynecology, §Department of Physiology, ∥Cardiovascular Research Centre, and ⊥Women and Children's Health Research Institute, University of Alberta , Edmonton, Alberta T6G 2P5, Canada.
authors.forename: Jianping
authors.initials: J
authors.lastname: Wu
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D003207:Conalbumin / Q000737:chemistry*; D004527:Egg Proteins / Q000737:chemistry*; D004529:Egg White / Q000737:chemistry*; D007249:Inflammation; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D012084:Renin-Angiotensin System; D013481:Superoxides / Q000378:metabolism
chemical_list: D000959:Antihypertensive Agents; D004527:Egg Proteins; D016328:NF-kappa B; D013481:Superoxides; D000804:Angiotensin II; D003207:Conalbumin
keywords: IRW; VSMC; inflammation; oxidative stress; proliferation