title: Description of Gabonibacter massiliensis gen. nov., sp. nov., a New Member of the Family Porphyromonadaceae Isolated from the Human Gut Microbiota.
abstract: The identification of human-associated bacteria is very important to control infectious diseases. In recent years, we diversified culture conditions in a strategy named culturomics, and isolated more than 100 new bacterial species and/or genera. Using this strategy, strain GM7, a strictly anaerobic gram-negative bacterium was recently isolated from a stool specimen of a healthy Gabonese patient. It is a motile coccobacillus without catalase and oxidase activities. The genome of Gabonibacter massiliensis is 3,397,022 bp long with 2880 ORFs and a G+C content of 42.09 %. Of the predicted genes, 2,819 are protein-coding genes, and 61 are RNAs. Strain GM7 differs from the closest genera within the family Porphyromonadaceae both genotypically and in shape and motility. Thus, we propose that strain GM7T(=CSUR P2336 = DSM 101039) is the type strain of the new genus Gabonibacter gen. nov. and the new species G. massiliensis gen. nov., sp. nov.
authors.affiliation: URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée-Infection, Faculté de Médecine, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille, France.|Ecole Doctorale Régionale d'Afrique Centrale, B.P. 876, Franceville, Gabon.
authors.forename: Gaël
authors.initials: G
authors.lastname: Mourembou
authors.affiliation: URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée-Infection, Faculté de Médecine, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille, France.
authors.forename: Jaishriram
authors.initials: J
authors.lastname: Rathored
authors.affiliation: Unité de Parasitologie Médicale (UPARAM), CIRMF B.P. 769, Franceville, Gabon.|Département de Parasitologie Mycologie et de Médecine Tropicale, Université des Sciences de la Santé, B.P. 4009, Libreville, Gabon.
authors.forename: Jean Bernard
authors.initials: JB
authors.lastname: Lekana-Douki
authors.affiliation: Département de Microbiologie, Laboratoire national de référence IST/sida, Faculté de Médecine, Université des Sciences de la Santé, B.P. 4009, Libreville, Gabon.
authors.forename: Angélique
authors.initials: A
authors.lastname: Ndjoyi-Mbiguino
authors.affiliation: URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée-Infection, Faculté de Médecine, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille, France.
authors.forename: Saber
authors.initials: S
authors.lastname: Khelaifia
authors.affiliation: URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée-Infection, Faculté de Médecine, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Robert
authors.affiliation: URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée-Infection, Faculté de Médecine, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille, France.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Armstrong
authors.affiliation: URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée-Infection, Faculté de Médecine, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille, France.|Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.affiliation: URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée-Infection, Faculté de Médecine, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille, France. pierre-edouard.fournier@univ-amu.fr.
authors.forename: Pierre-Edouard
authors.initials: PE
authors.lastname: Fournier
mesh_terms: D000293:Adolescent; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D000069196:Gastrointestinal Microbiome; D041981:Gastrointestinal Tract / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D008297:Male; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Culturomics; Gabon; Gabonibacter massiliensis; Gut microbiota; Taxonogenomics