title: Seed Oil of Brucea javanicaInduces Cell Cycle Arrest and Apoptosis via Reactive Oxygen Species-Mediated Mitochondrial Dysfunction in Human Lung Cancer Cells.
abstract: Brucea javanica oil (BJO), a traditional herbal medicine extracted from the seeds of B. javanica, has been clinically used to treat non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC) in combination with chemotherapy or radiotherapy in China. However, how BJO exerts this antitumor effect is still largely unknown. Here, effects of BJO on the growth of NSCLC and SCLC cell lines were investigated by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenytetrazolium Bromide (MTT) assay, and the results showed that BJO inhibited the proliferation of A549 cells (NSCLC) and H446 cells (SCLC). Further studies revealed that BJO induced G0/G1 arrest partly via regulating p53 and cyclin D1 in these two cell lines. BJO also has pro-apoptotic effect on H446 and A549 cells through mitochondria/caspase-mediated pathway, which was initiated by the accumulation of intracellular reactive oxygen species (ROS). These findings thus revealed the molecular mechanisms underlying the antitumor effect of BJO on SCLC and NSCLC, which may benefit the further clinical application of BJO.
authors.affiliation: a Provincial Clinical Medical College , Fujian Medical University , Fuzhou , Fujian , China.
authors.forename: Daxuan
authors.initials: D
authors.lastname: Wang
authors.affiliation: b Cancer Research Center , Medical College of Xiamen University , Xiamen , Fujian , China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Qu
authors.affiliation: c Department of Respiratory Medicine , First Hospital of Quanzhou Affiliated to Fujian Medical University , Quanzhou , Fujian , China.
authors.forename: Xibin
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: d Department of Thoracic Surgery , First Affiliated Hospital of Xiamen University , Xiamen , Fujian , China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Geng
authors.affiliation: b Cancer Research Center , Medical College of Xiamen University , Xiamen , Fujian , China.
authors.forename: Jihuan
authors.initials: J
authors.lastname: Hou
authors.affiliation: a Provincial Clinical Medical College , Fujian Medical University , Fuzhou , Fujian , China.
authors.forename: Nengluan
authors.initials: N
authors.lastname: Xu
authors.affiliation: c Department of Respiratory Medicine , First Hospital of Quanzhou Affiliated to Fujian Medical University , Quanzhou , Fujian , China.
authors.forename: Wenlan
authors.initials: W
authors.lastname: Li
authors.affiliation: b Cancer Research Center , Medical College of Xiamen University , Xiamen , Fujian , China.
authors.forename: Tianhui
authors.initials: T
authors.lastname: Hu
authors.affiliation: a Provincial Clinical Medical College , Fujian Medical University , Fuzhou , Fujian , China.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Chen
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D032269:Brucea / Q000737:chemistry*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000473:pathology; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects*; D010938:Plant Oils / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D055752:Small Cell Lung Carcinoma / Q000188:drug therapy / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C530104:CCND1 protein, human; D010938:Plant Oils; D017382:Reactive Oxygen Species; D019938:Cyclin D1