title: Mining bifidobacteria from the neonatal gastrointestinal tract for conjugated linolenic acid production.
abstract: Conjugated linolenic acid (CLNA) is a family of isomers of linolenic acid with a number of health-associated benefits, which has been attracting great interest. Microbial CLNA producers are potentially an alternative source of CLNA for human nutrition. In present study, 16 neonate feces were collected and used for Bifidobacteria isolation, from which 25 bifidobacteria isolates were obtained. The bifidobacteria isolates were identified using 16s rDNA sequencing as Bifidobacterium adolescentis, B. breve, B. longum and B. pseudocatenulatum. These isolates were further investigated for their ability to produce CLNA using linolenic acid as substrate via GC-MS. The results showed most of the isolates could convert free linolenic acid into c9,t11,c15-CLNA and t9,t11,c15-CLNA at different levels. B. pseudocatenulatum was the most effective CLNA producer, which converted 86.91% of linolenic acid to c9,t11,c15-CLNA and 3.59% of to t9,t11,c15-CLNA isomer and the isolate exhibited to accumulate CLNA during 72 h culturing in which most CLNA isomers were in the supernatant fluid. The results indicated that utilization of this isolate for CLNA production will eliminate the purification process.
authors.affiliation: a State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , Jiangsu , P. R China.|b International Joint Research Laboratory for Probiotics at Jiangnan University , Wuxi , Jiangsu , P. R China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: a State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , Jiangsu , P. R China.
authors.forename: Haiqin
authors.initials: H
authors.lastname: Chen
authors.affiliation: b International Joint Research Laboratory for Probiotics at Jiangnan University , Wuxi , Jiangsu , P. R China.|c Teagasc Moorepark Food Research Center , Fermoy , Co. Cork , Ireland.
authors.forename: Catherine
authors.initials: C
authors.lastname: Stanton
authors.affiliation: a State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , Jiangsu , P. R China.
authors.forename: Yong Q
authors.initials: YQ
authors.lastname: Chen
authors.affiliation: a State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , Jiangsu , P. R China.|b International Joint Research Laboratory for Probiotics at Jiangnan University , Wuxi , Jiangsu , P. R China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Wuxi , Jiangsu , P. R China.|b International Joint Research Laboratory for Probiotics at Jiangnan University , Wuxi , Jiangsu , P. R China.|d Beijing Innovation Center of Food Nutrition and Human Health , Beijing Technology and Business University (BTBU) , Beijing , P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D001644:Bifidobacterium / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007231:Infant, Newborn; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis* / Q000302:isolation & purification; D008297:Male; D013045:Species Specificity; D013048:Specimen Handling / Q000379:methods*
chemical_list: D044243:Linoleic Acids, Conjugated
keywords: bifidobacteria; bioconversion; conjugated linolenic acid; isolation