title: Imaging Golgi Outposts in Fixed and Living Neurons.
abstract: Here we describe the use of confocal microscopy in combination with antibodies specific to Golgi proteins to visualize dendritic Golgi outposts (GOPs) in cultured hippocampal pyramidal neurons. We also describe the use of spinning disk confocal microscopy, in combination with ectopically expressed glycosyltransferases fused to GFP variants, to visualize GOPs in living neurons.
authors.affiliation: Laboratorio Neurobiología, Instituto Investigación Médica Mercedes y Martín Ferreyra (INIMEC-CONICET), Av. Friuli 2434, 5016, Córdoba, Argentina.|Universidad Nacional de Córdoba (Argentina), Av. Haya de la Torre s/n, 5000, Córdoba, Argentina.|Instituto Universitario Ciencias Biomédicas Córdoba, Av. Friuli 2786, 5016, Córdoba, Argentina.
authors.forename: Mariano
authors.initials: M
authors.lastname: Bisbal
authors.affiliation: Laboratorio Neurobiología, Instituto Investigación Médica Mercedes y Martín Ferreyra (INIMEC-CONICET), Av. Friuli 2434, 5016, Córdoba, Argentina.|Universidad Nacional de Córdoba (Argentina), Av. Haya de la Torre s/n, 5000, Córdoba, Argentina.|Instituto Universitario Ciencias Biomédicas Córdoba, Av. Friuli 2786, 5016, Córdoba, Argentina.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Quassollo
authors.affiliation: Laboratorio Neurobiología, Instituto Investigación Médica Mercedes y Martín Ferreyra (INIMEC-CONICET), Av. Friuli 2434, 5016, Córdoba, Argentina. acaceres@immf.uncor.edu.|Universidad Nacional de Córdoba (Argentina), Av. Haya de la Torre s/n, 5000, Córdoba, Argentina. acaceres@immf.uncor.edu.|Instituto Universitario Ciencias Biomédicas Córdoba, Av. Friuli 2786, 5016, Córdoba, Argentina. acaceres@immf.uncor.edu.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Caceres
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D017966:Pyramidal Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D000906:Antibodies
keywords: Confocal microscopy; Cultures; Dendrites; Golgi outposts; Live imaging; Neurons