title: Tissue transglutaminase-1 promotes stemness and chemoresistance in gastric cancer cells by regulating Wnt/β-catenin signaling.
abstract: Gastric cancer is a common malignancy, and is one of the most frequent causes of cancer deaths worldwide. Recently, members of the transglutaminases (TGM) family, especially TGM2, have been implicated in the progression and drug resistance of cancers, but the function of TGM1 in cancer development has been largely overlooked. In this study, we demonstrate the roles of TGM1 in development of gastric cancer. We found that expression levels of TGM1 were upregulated in both gastric cancer tissues and cultured gastric cancer cells, and that TGM1 expression levels were correlated with patient survival. In cultured gastric cancer cells, loss of TGM1 expression inhibited cell proliferation and promoted apoptosis, as well increased gastric cancer cell sensitivity to chemotherapeutic drugs and reducing stemness. These results strongly supported the participation of TGM1 in the regulation of gastric cancer development. In addition, we found evidence that the mechanism of action of TGM1 in regulating gastric cancer cell might involve the Wnt signaling pathway, as loss of TGM1 expression in gastric cancer cells led to a significant suppression of Wnt signaling activities.
authors.affiliation: 1 Department of General Surgery, Oilfield General Hospital of Daqing, Daqing 163001, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Huang
authors.affiliation: 1 Department of General Surgery, Oilfield General Hospital of Daqing, Daqing 163001, China.
authors.forename: Zhiqi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: 2 Department of Anatomy, Harbin Medical University-Daqing, Daqing 163319, China.
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Ni
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D002945:Cisplatin / Q000494:pharmacology; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D011503:Transglutaminases / Q000235:genetics / Q000378:metabolism*; D014750:Vincristine / Q000494:pharmacology; D051153:Wnt Proteins / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000502:physiology*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D034741:RNA, Small Interfering; D051153:Wnt Proteins; D051176:beta Catenin; D014750:Vincristine; D011503:Transglutaminases; C105804:transglutaminase 1; D002945:Cisplatin; D005472:Fluorouracil
keywords: Gastric cancer; Wnt signaling; chemoresistance; stemness; transglutaminase-1