title: Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial Cells.
abstract: The potential use of stem cells as therapeutics in disease has gained momentum over the last few years and recently phase-I clinical trials have shown favourable results in treatment of a small cohort of acute stroke patients. Similarly, they have been used in preclinical models drug-loaded for the effective treatment of solid tumours. Here we have characterized uptake and release of a novel p5-cyclin-dependent kinase 5 (CDK5) inhibitory peptide by mesenchymal stem cells and showed release levels capable of blocking aberrant cyclin-dependent kinase 5 (CDK5) signaling pathways, through phosphorylation of cyclin-dependent kinase 5 (CDK5) and p53. These pathways represent the major acute mechanism stimulating apoptosis after stroke and hence its modulation could benefit patient recovery. This work indicates a potential use for drug-loaded stem cells as delivery vehicles for stroke therapeutics and in addition as anticancer receptacles particularly, if a targeting and/or holding mechanism can be defined.
authors.affiliation: School of Healthcare Science, Manchester Metropolitan University, Manchester, UK.
authors.forename: Wen-Hui
authors.identifier: 0000-0002-5549-8219
authors.initials: WH
authors.lastname: Fang
authors.affiliation: School of Healthcare Science, Manchester Metropolitan University, Manchester, UK.
authors.forename: Shant
authors.initials: S
authors.lastname: Kumar
authors.affiliation: School of Healthcare Science, Manchester Metropolitan University, Manchester, UK.
authors.forename: Garry
authors.initials: G
authors.lastname: McDowell
authors.affiliation: School of Healthcare Science, Manchester Metropolitan University, Manchester, UK.
authors.forename: David
authors.identifier: 0000-0001-5495-7981
authors.initials: D
authors.lastname: Smith
authors.affiliation: Department of Neurology, Hospital Universitari Mútua de Terrassa, Terrassa, Barcelona, Spain.
authors.forename: Jurek
authors.initials: J
authors.lastname: Krupinski
authors.affiliation: University of Medicine and Pharmacy, Tirgu Mures, Romania.
authors.forename: Peter
authors.initials: P
authors.lastname: Olah
authors.affiliation: Department of Medical Laboratories, College of Applied Medical Sciences, Majmaah University, Al Majma'ah, Saudi Arabia.
authors.forename: Raid Saleem
authors.initials: RS
authors.lastname: Al-Baradie
authors.affiliation: Department of Medical Laboratories, College of Applied Medical Sciences, Majmaah University, Al Majma'ah, Saudi Arabia.
authors.forename: Mohammad Othman
authors.initials: MO
authors.lastname: Al-Rukban
authors.affiliation: Griffith University School of Medicine and Queensland Eye Institute, Griffith University, Nathan, QLD, Australia.
authors.forename: Eugene Bogdan
authors.initials: EB
authors.lastname: Petcu
authors.affiliation: School of Healthcare Science, Manchester Metropolitan University, Manchester, UK; University of Medicine and Pharmacy, Tirgu Mures, Romania.
authors.forename: Mark
authors.identifier: 0000-0003-3767-4861
authors.initials: M
authors.lastname: Slevin