title: Identification of QTLs associated with tissue culture response of mature wheat embryos.
abstract: Mature embryo is an excellent explant for tissue culture as it is convenient to be obtained without limitation of growing seasons and development stages. However, regeneration ability of the calli from wheat mature embryos is limited, thus hindering its application. To identify genes associated with the tissue culture response (TCR) of wheat, QTLs for callus induction from mature embryos and callus regeneration were detected using a recombinant inbred lines (RILs) population derived from the cross between a synthetic hexaploid wheat genotype, SHW-L1 and a commercial cultivar Chuanmai 32. Three QTLs for callus rate were identified and they were located on chromosomes 1D, 5A, and 6D, respectively, with explained phenotypic variation ranging from 10.16 to 11.82 %. One QTL for differentiation rate was detected only with 10.96 % of the phenotypic variation explained. Two QTLs for emergence rate were identified and they were located on 3B and 4A, respectively, with 9.88 and 10.30 % of phenotypic variation. The results presented in this study with those reported previously indicated that group 1, 3, and 5 chromosomes are likely to play important roles in TCR of wheat.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130 Sichuan China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130 Sichuan China.
authors.forename: Mei
authors.initials: M
authors.lastname: Deng
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130 Sichuan China.
authors.forename: Si-Yu
authors.initials: SY
authors.lastname: Lv
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130 Sichuan China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130 Sichuan China.
authors.forename: Qian-Tao
authors.initials: QT
authors.lastname: Jiang
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130 Sichuan China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Qi
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130 Sichuan China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130 Sichuan China.
authors.forename: Guo-Yue
authors.initials: GY
authors.lastname: Chen
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130 Sichuan China.
authors.forename: Xiu-Jin
authors.initials: XJ
authors.lastname: Lan
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130 Sichuan China.
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Wei
keywords: Callus rate; Differentiation; Plant regeneration rate; QTL; Wheat