title: Genomewide analysis of MATE-type gene family in maize reveals microsynteny and their expression patterns under aluminum treatment.
abstract: Multidrug and toxic compound extrusion (MATE) proteins are a group of secondary active transporters, which widely exist in all living organisms and play important role in the detoxication of endogenous secondary metabolites and exogenous agents. However, to date, no systematic and comprehensive study of this family is reported in maize. Here, a total of 49 MATE genes (ZmMATE) were identified and divided into seven groups by phylogenetic analysis. Conserved intro-exon structures and motif compositions were investigated in these genes. Results by gene locations indicated that these genes were unevenly distributed among all 10 chromosomes. Tandem and segmental duplications appeared to contribute to the expansion and evolution of this gene family. The Ka/Ks ratios suggested that the ZmMATE has undergone large-scale purifying selection on the maize genome. Interspecies microsynteny analysis revealed that there were independent gene duplication events of 10 ZmMATE. In addition, most maize MATE genes exhibited different expression profiles in diverse tissues and developmental stages. Sixteen MATE genes were chosen for further quantitative real-time polymerase chain reaction analysis showed differential expression patterns in response to aluminum treatment. These results provide a useful clue for future studies on the identification of MATE genes and functional analysis of MATE proteins in maize.
authors.affiliation: Key Laboratory of Crop Biology of Anhui Province, Anhui Agricultural University, Hefei 230036, People's Republic of China.chengbeijiu2007@163.com.
authors.forename: Huasheng
authors.initials: H
authors.lastname: Zhu
authors.forename: Jiandong
authors.initials: J
authors.lastname: Wu
authors.forename: Yingli
authors.initials: Y
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Guomin
authors.initials: G
authors.lastname: Han
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Beijiu
authors.initials: B
authors.lastname: Cheng
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000595:Amino Acid Sequence; D032461:Chromosomes, Plant / Q000737:chemistry*; D017124:Conserved Sequence; D005091:Exons; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D018745:Genome, Plant; D007438:Introns; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D059372:Nucleotide Motifs; D027701:Organic Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D056916:Segmental Duplications, Genomic; D012641:Selection, Genetic; D026801:Synteny; D003313:Zea mays / Q000145:classification / Q000187:drug effects* / Q000235:genetics
chemical_list: D027701:Organic Cation Transport Proteins; D010940:Plant Proteins; D000535:Aluminum