title: Method for In-Vivo Fluorescence Imaging Contrast Enhancement through Light Modulation.
abstract: Early diagnosis is one of the most important factors that increase the therapeutic potential of the disease. Diagnoses conducted by conventional equipment are expensive, time-consuming, burdensome to patients, and do not have high success rates. Diagnostic methods have also been investigated using nanoparticles. However, there have been no significant improvements in the early diagnosis of disease. The diagnosis technique proposed in this paper consumes less time, is more cost-effective, and more accurate. It uses a new concept-a low-intensity fluorescence molecular imaging system with a lock-in technique. This study applied the lock-in technique to basic research in contrast enhancement and optimization. This improved fluorescence distribution analysis, resulting in increased resolution of optical molecular imaging for early diagnosis of disease. An experimental lock-in fluorescence imaging system, which used a variety of fluorescent dyes, achieved signal amplification 100 times greater than that of a conventional fluorescence imaging system. The results of this study demonstrate that the lock-in technique could significantly improve optical molecular imaging technology, making it possible to achieve early diagnosis of disease.
authors.affiliation: Research Institute for Skin Image, College of Medicine, Korea University, Seoul, South Korea.
authors.forename: Jaeyoung
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Medical IT Engineering, College of Medical Sciences, Soonchunhyang University, Chungnam, South Korea.
authors.forename: Onseok
authors.initials: O
authors.lastname: Lee
authors.affiliation: Department of Nursing, School of Health, Chungbuk Health and Science University, Chungbuk, South Korea.
authors.forename: Seunghan
authors.initials: S
authors.lastname: Ha
authors.affiliation: Department of Dermatology, College of Medicine, Korea University, Seoul, South Korea.
authors.forename: Jung Woo
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Research Institute for Skin Image, College of Medicine, Korea University, Seoul, South Korea. choh@korea.ac.kr.|Department of Dermatology, College of Medicine, Korea University, Seoul, South Korea. choh@korea.ac.kr.
authors.forename: Chilhwan
authors.initials: C
authors.lastname: Oh
mesh_terms: D005456:Fluorescent Dyes; D006801:Humans; D008027:Light; D057054:Molecular Imaging; D053758:Nanoparticles; D061848:Optical Imaging
chemical_list: D005456:Fluorescent Dyes
keywords: Contrast enhancement; Fluorescent dye; Lock-in technique; Nanoparticle