title: Addition of Surfactants and Non-Hydrolytic Proteins and Their Influence on Enzymatic Hydrolysis of Pretreated Sugarcane Bagasse.
abstract: Poly(ethylene glycol) (PEG 4000) and bovine serum albumin (BSA) were investigated with the purpose of evaluating their influence on enzymatic hydrolysis of sugarcane bagasse. Effects of these supplements were assayed for different enzymatic cocktails (Trichoderma harzianum and Penicillium funiculosum) that acted on lignocellulosic material submitted to different pretreatment methods with varying solid (25 and 100 g/L) and protein (7.5 and 20 mg/g cellulose) loadings. The highest levels of glucose release were achieved using partially delignified cellulignin as substrate, along with the T. harzianum cocktail: increases of 14 and 18 % for 25 g/L solid loadings and of 33 and 43 % for 100 g/L solid loadings were reached for BSA and PEG supplementation, respectively. Addition of these supplements could maintain hydrolysis yield even for higher solid loadings, but for higher enzymatic cocktail protein loadings, increases in glucose release were not observed. Results indicate that synergism might occur among these additives and cellulase and xylanases. The use of these supplements, besides depending on factors such as pretreatment method of sugarcane bagasse, enzymatic cocktails composition, and solid and protein loadings, may not always lead to positive effects on the hydrolysis of lignocellulosic material, making it necessary further statistical studies, according to process conditions.
authors.affiliation: Laboratories of Bioprocess Development, School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil.
authors.forename: Johanna
authors.initials: J
authors.lastname: Méndez Arias
authors.affiliation: Laboratory of Biotechnology and Environment, School of Engineering, Fluminense Federal University, Rio de Janeiro, 24210-310, Brazil.
authors.forename: Anelize
authors.initials: A
authors.lastname: de Oliveira Moraes
authors.affiliation: Laboratories of Bioprocess Development, School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil.
authors.forename: Luiz Felipe Amarante
authors.initials: LF
authors.lastname: Modesto
authors.affiliation: Biotechnology Department, Research and Development Center, PETROBRAS, Rio de Janeiro, 21941-920, Brazil.
authors.forename: Aline Machado
authors.initials: AM
authors.lastname: de Castro
authors.affiliation: Laboratories of Bioprocess Development, School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil. nei@eq.ufrj.br.
authors.forename: Nei
authors.initials: N
authors.lastname: Pereira
mesh_terms: D044602:Cellulases / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D005947:Glucose / Q000138:chemical synthesis*; D006358:Hot Temperature; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D010407:Penicillium / Q000201:enzymology; D011092:Polyethylene Glycols / Q000737:chemistry; D031786:Saccharum / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*; D014242:Trichoderma / Q000201:enzymology
chemical_list: D013464:Sulfuric Acids; D013501:Surface-Active Agents; C036909:lignocellulose; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; C000595214:polyethylene glycol 4000; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D044602:Cellulases; D005947:Glucose; C033158:sulfuric acid
keywords: Additives; Biorefinery; Cellulase; Enzymatic hydrolysis; Lignocellulosic biomass; Synergism