title: Cell-free DNA in pregnancy with choriocarcinoma and coexistent live fetus: A case report.
abstract: BACKGROUND
  This case report describes the use of analysis of cell-free DNA in the blood of a patient with a pregnancy with one live fetus and a choriocarcinoma diagnosed at 22 weeks of gestation.
  
  
  RESULTS
  The result of the analysis of 16 microsatellite loci on 14 chromosomes in the cell-free DNA in plasma was consistent with the result of the analysis of a tumor biopsy indicating biparental diploid origin of the genome. The DNA markers were discordant with the markers of the placenta indicating two separate conceptions.
  
  
  CONCLUSIONS
  Our results indicate that analysis of cell-free DNA in plasma allows determination of the origin of a choriocarcinoma without tissue biopsy, even in the presence of a co-existent pregnancy.
authors.affiliation: Department of Clinical Genetics, Aarhus University Hospital, Aarhus N Department of Gynecology and Obstetrics, Aarhus University Hospital, Aarhus N Department of Oncology, Aarhus University Hospital, Aarhus C Department of Clinical Immunology, Aarhus University Hospital, Aarhus N Institute of Pathology, Aarhus University Hospital, Aarhus C Department of Biomedicine, Aarhus University, Aarhus C, Denmark.
authors.forename: Mona Kjaerboel
authors.initials: MK
authors.lastname: Kristiansen
authors.forename: Isa
authors.initials: I
authors.lastname: Niemann
authors.forename: Jacob Christian
authors.initials: JC
authors.lastname: Lindegaard
authors.forename: Mette
authors.initials: M
authors.lastname: Christiansen
authors.forename: Mette Warming
authors.initials: MW
authors.lastname: Joergensen
authors.forename: Ida
authors.initials: I
authors.lastname: Vogel
authors.forename: Dorte Launholt
authors.initials: DL
authors.lastname: Lildballe
authors.forename: Lone
authors.initials: L
authors.lastname: Sunde
mesh_terms: D000328:Adult; D002822:Choriocarcinoma / Q000097:blood*; D004247:DNA / Q000097:blood*; D005260:Female; D006801:Humans; D050498:Live Birth; D011247:Pregnancy
chemical_list: D004247:DNA