title: Capillary Electrophoresis of Mono- and Oligosaccharides.
abstract: This chapter reports an overview of the recent advances in the analysis of mono- and oligosaccharides by capillary electrophoresis (CE); furthermore, relevant reviews and research articles recently published in the field are tabulated. Additionally, pretreatments and procedures applied to uncharged and acidic carbohydrates (i.e., monosaccharides and lower oligosaccharides carrying carboxylate, sulfate, or phosphate groups) are described.Representative examples of such procedures are reported in detail, upon describing robust methodologies for the study of (1) neutral oligosaccharides derivatized by reductive amination and by formation of glycosylamines; (2) sialic acid derivatized with 2-aminoacridone, released from human serum immunoglobulin G; (3) anomeric couples of neutral glycosides separated using borate-based buffers; (4) unsaturated, underivatized oligosaccharides from lyase-treated alginate.
authors.affiliation: GSK Vaccines, Manufacturing Science & Technology Bellaria di Rosia, Sovicille (Siena), Italy.
authors.forename: Mila
authors.initials: M
authors.lastname: Toppazzini
authors.affiliation: GSK Vaccines, Manufacturing Science & Technology Bellaria di Rosia, Sovicille (Siena), Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Coslovi
authors.affiliation: Bracco Imaging SpA-CRB Trieste, AREA Science Park, Trieste, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Rossi
authors.affiliation: Bracco Imaging SpA-CRB Trieste, AREA Science Park, Trieste, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Flamigni
authors.affiliation: Bracco Imaging SpA-CRB Trieste, AREA Science Park, Trieste, Italy.
authors.forename: Edi
authors.initials: E
authors.lastname: Baiutti
authors.affiliation: GSK Vaccines, Manufacturing Science & Technology Bellaria di Rosia, Sovicille (Siena), Italy. cristiana.x.campa@gsk.com.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Campa
mesh_terms: D002241:Carbohydrates / Q000097:blood* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006027:Glycosides / Q000097:blood / Q000737:chemistry; D006801:Humans; D009005:Monosaccharides / Q000097:blood* / Q000737:chemistry; D019158:N-Acetylneuraminic Acid / Q000097:blood / Q000737:chemistry; D009844:Oligosaccharides / Q000097:blood* / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D006027:Glycosides; D009005:Monosaccharides; D009844:Oligosaccharides; D019158:N-Acetylneuraminic Acid
keywords: Alditols; Capillary electrophoresis; Carboxylated sugars; Glycosaminoglycans oligosaccharides; Glycosides; Glycosylamines; Monosaccharides; Neutral sugars; Oligosaccharides; Reductive amination; Sialic acids; Sugar acids; Sugar phosphates; Sulfated sugars; Uronic acids