title: Multiple biological processes may be associated with tumorigenesis under NUP88-overexpressed condition.
abstract: Overexpression of the nucleoporin NUP88 has been observed in a large number of tumors and has been experimentally proven to promote tumorigenesis. However, the mechanism underlying the tumor-promoting activity of overexpressed NUP88 is not clear. To investigate the potential pathways that drive tumorigenesis under NUP88 overexpressed condition, we applied a proteomic approach to identify NUP88-associated proteins at a subcellular compartment level. Gene ontology analysis revealed significant associations between NUP88 interactome and biological processes that are related to nuclear transport, RNA processing, cell cycle progression, metabolic regulation, and viral infection. Moreover, we found that NUP88 interacts with MISP, a mitotic interactor and substrate of PLK1. Interestingly, NUP88 overexpression blocks MISP phosphorylation, which is known to be critical for normal spindle formation and accurate chromosome segregation during mitosis. In conclusion, our data for the first time provide a global view of biological processes that may drive tumorigenesis under NUP88 overexpressed condition, revealing a biological effect of NUP88-MISP interaction. Furthermore, identification of NUP88-associated proteins provides a valuable database for future studies. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Anaesthesia, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of cancer prevention and therapy, Tianjin, China.|National Clinical Research Center for Cancer, Tianjin, China.
authors.forename: Jiafeng
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, Tianjin Nankai Hospital, Nankai Clinical School of Tianjin Medical University, Tianjin, China.
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Anaesthesia, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of cancer prevention and therapy, Tianjin, China.|National Clinical Research Center for Cancer, Tianjin, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology*; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D061986:MCF-7 Cells; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; C104835:NUP88 protein, human; D028861:Nuclear Pore Complex Proteins; D020543:Proteome