title: Extraction, characterization and bioactivities of novel purified polysaccharides from Baphicacanthis Cusiae Rhizoma et Radix.
abstract: The purpose of this study was to investigate the extraction, characterization and bioactivities of purified water-soluble polysaccharides (BCP) from Baphicacanthis Cusiae Rhizoma et Radix. Based on the response surface methodology, the optimal extraction parameters were obtained as follows: extraction temperature of 60.0°C, extraction time of 35.0min, and ratio of water to raw material of 24.5ml/g. Then, BCP was separated and purified by chromatography of DEAE-52 and Sephadex G-100, and obtained two purified fractions, named as BCP-1 and BCP-2. Their molecular weights were respectively 11.6 and 26.7 KDa with mainly composed of glucose, arabinose and galactose. BCP-2 had higher contents of sulfuric radical and uronic acid than BCP-1. Finally, their antioxidant and anti-inflammatory activities were evaluated. Both of BCP-1 and BCP-2 exhibited strong antioxidant activity in vitro, and the antioxidant of BCP-2 was better. Besides, they showed ideal anti-inflammatory activity in vitro and in vivo.
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Du
authors.affiliation: Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, Jiangsu, PR China.
authors.forename: Baolan
authors.initials: B
authors.lastname: Wang
authors.affiliation: Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an 223001, Jiangsu, PR China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Chen
authors.affiliation: Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, Jiangsu, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Li
authors.affiliation: Xinhua College, Sun Yat-sen University, Guangzhou 510520, Guangdong, PR China.
authors.forename: Lian
authors.initials: L
authors.lastname: He
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Lai
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China. Electronic address: danyan64@163.com.
authors.forename: Danyan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Pingguang Pharmaceutical Co., Ltd., Hefei 231131, PR China.
authors.forename: Kanglin
authors.initials: K
authors.lastname: Wang
mesh_terms: D027901:Acanthaceae / Q000737:chemistry; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004353:Drug Evaluation, Preclinical; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007502:Iron Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000067996:RAW 264.7 Cells; D027343:Rhizome / Q000737:chemistry; D014992:Xylenes
chemical_list: D000893:Anti-Inflammatory Agents; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D010936:Plant Extracts; D011134:Polysaccharides; D014992:Xylenes; D009569:Nitric Oxide
keywords: Anti-inflammatory activity; Baphicacanthis Cusiae Rhizoma et Radix; Polysaccharide