title: Comparative analysis of the immunomodulatory capacities of human bone marrow- and adipose tissue-derived mesenchymal stromal cells from the same donor.
abstract: The immunomodulatory properties of mesenchymal stromal cells (MSCs), together with their tissue regenerative potential, make them interesting candidates for clinical application.
  
  
  
  In the current study, we analyzed the in vitro immunomodulatory effects of MSCs derived from bone marrow (BM-MSCs) and from adipose tissue (AT-MSCs) obtained from the same donor on both innate and acquired immunity cells. BM-MSCs and AT-MSCs were expanded to fourth or fifth passage and co-cultured with T cells, monocytes or natural killer (NK) cells isolated from human peripheral blood and stimulated in vitro. The possible differing impact of MSCs obtained from distinct sources on phenotype, cell proliferation and differentiation, cytokine production and function of these immune cells was comparatively analyzed.
  
  
  
  BM-MSCs and AT-MSCs induced a similar decrease in NK-cell proliferation, cytokine secretion and expression of both activating receptors and cytotoxic molecules. However, only BM-MSCs significantly reduced NK-cell cytotoxic activity, although both MSC populations showed the same susceptibility to NK-cell-mediated lysis. AT-MSCs were more potent in inhibiting dendritic-cell (DC) differentiation than BM-MSC, but both MSC populations similarly reduced the ability of DCs to induce CD4(+) T-cell proliferation and cytokine production. BM-MSCs and AT-MSCs induced a similar decrease in T-cell proliferation and production of inflammatory cytokines after activation.
  
  
  
  AT-MSCs and BM-MSCs from the same donor had similar immunomodulatory capacity on both innate and acquired immunity cells. Thus, other variables, such as accessibility of samples or the frequency of MSCs in the tissue should be considered to select the source of MSC for cell therapy.
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain. Electronic address: jarisval@ucm.es.
authors.forename: Jaris
authors.initials: J
authors.lastname: Valencia
authors.affiliation: Department of Hematology, IBSAL-Hospital Universitario de Salamanca, Salamanca, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Blanco
authors.affiliation: Hematopoietic Innovative Therapies Division, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT) and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBER-ER), Madrid, Spain; Advanced Therapies Mixed Unit, Instituto de Investigación Sanitaria-Fundación Jiménez Díaz (IIS-FJD), Madrid, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Yáñez
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: Vázquez
authors.affiliation: Department of Hematology, IBSAL-Hospital Universitario de Salamanca, Salamanca, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Herrero Sánchez
authors.affiliation: Hematopoietic Innovative Therapies Division, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT) and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBER-ER), Madrid, Spain; Advanced Therapies Mixed Unit, Instituto de Investigación Sanitaria-Fundación Jiménez Díaz (IIS-FJD), Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Fernández-García
authors.affiliation: Department of Hematology, IBSAL-Hospital Universitario de Salamanca, Salamanca, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: Rodríguez Serrano
authors.affiliation: Department of Hematology, IBSAL-Hospital Universitario de Salamanca, Salamanca, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Pescador
authors.affiliation: Department of Hematology, IBSAL-Hospital Universitario de Salamanca, Salamanca, Spain.
authors.forename: Juan F
authors.initials: JF
authors.lastname: Blanco
authors.affiliation: Hematopoietic Innovative Therapies Division, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT) and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBER-ER), Madrid, Spain; Advanced Therapies Mixed Unit, Instituto de Investigación Sanitaria-Fundación Jiménez Díaz (IIS-FJD), Madrid, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: Hernando-Rodríguez
authors.affiliation: Department of Hematology, IBSAL-Hospital Universitario de Salamanca, Salamanca, Spain.
authors.forename: Fermín
authors.initials: F
authors.lastname: Sánchez-Guijo
authors.affiliation: Hematopoietic Innovative Therapies Division, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT) and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBER-ER), Madrid, Spain; Advanced Therapies Mixed Unit, Instituto de Investigación Sanitaria-Fundación Jiménez Díaz (IIS-FJD), Madrid, Spain.
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Lamana
authors.affiliation: Hematopoietic Innovative Therapies Division, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT) and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBER-ER), Madrid, Spain; Advanced Therapies Mixed Unit, Instituto de Investigación Sanitaria-Fundación Jiménez Díaz (IIS-FJD), Madrid, Spain.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Segovia
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Complutense University, Madrid, Spain.
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Vicente
authors.affiliation: Department of Hematology, IBSAL-Hospital Universitario de Salamanca, Salamanca, Spain.
authors.forename: Consuelo
authors.initials: C
authors.lastname: Del Cañizo
authors.affiliation: Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, Spain.
authors.forename: Agustín G
authors.initials: AG
authors.lastname: Zapata
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D056747:Immunomodulation / Q000502:physiology*; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*; D014019:Tissue Donors
keywords: NK cell; T cell; adipose tissue; bone marrow; dendritic cell; human mesenchymal stromal cell; immunomodulation