title: In Vivo Interrelationship between Insulin Resistance and Interferon Gamma Production: Protective and Therapeutic Effect of Berberine.
abstract: This research was conducted to investigate if there is a relation between insulin resistance incidence and inhibition of interferon gamma production or not. Firstly, insulin resistance was induced by high fat diet (HFD) intake for 6 weeks. Secondly, berberine was used as protective/curative compound for insulin resistance. Results revealed that feeding rats HFD for 6 weeks developed features of insulin resistance (IR) syndrome. These features presented in increased body weight, hyperglycemia, hyperinsulinemia, hypercholesterolemia (with increased LDL-cholesterol and decreased HDL-cholesterol), and hypertriglyceridemia. Level of antioxidant enzymes in HFD group was higher than in normal one. Also there was an increasing in level of proinflammatory cytokines as interleukin- (IL-) 6 and IL-12 in HFD group. Feeding rats HFD for 6 weeks also decreased level of interferon gamma (IFN-γ). The decreased level of IFN-γ has been shown to predict infection with infectious diseases especially viral infection. Treatment and protection with berberine 50 mg/kg/day for 2 weeks were found to be effective against the features of insulin resistance syndrome, improved levels of insulin resistance parameters, lipid profile, antioxidant enzymes, proinflammatory cytokines, and IFN-γ.
authors.affiliation: Chemistry Department, Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh, Egypt.
authors.forename: Mohammad Ahmad
authors.identifier: 0000-0002-7693-1894
authors.initials: MA
authors.lastname: Mahmoud
authors.affiliation: Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
authors.forename: Doaa Ahmad
authors.identifier: 0000-0003-3327-4377
authors.initials: DA
authors.lastname: Ghareeb
authors.affiliation: Chemistry Department, Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh, Egypt.
authors.forename: Heba Abdelghany
authors.initials: HA
authors.lastname: Sahyoun
authors.affiliation: Chemistry Department, Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh, Egypt.
authors.forename: Ashraf Abdelhamed
authors.initials: AA
authors.lastname: Elshehawy
authors.affiliation: Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
authors.forename: Mohammad Mohammad
authors.initials: MM
authors.lastname: Elsayed