title: Identification and Characterization of Two New Degradation Products of Saikosaponin A under Acid Hydrolytic Conditions.
abstract: Saikosaponin (SS) A is a compound with various pharmacological properties and is easily degraded into SS-B1 and SS-G under acid conditions. In the present work, two new degradation products of SS-A, formed under acid hydrolytic conditions, were detected and isolated using analytical and semi-preparative liquid chromatography technology; furthermore, their structures were characterized as hydroxy-saikosaponin A and SS-B2 by spectral analysis, which is not only essential in stability-indicating method development and validation, but also could be used as a worst case scenario to assess the analytical method performance of SS-A. Moreover, their structural transformation pathways are also proposed.
authors.affiliation: College of Chemistry and Pharmaceutical Engineering, Henan University of Science and Technology, Luoyang 471023, China. huomanlee@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Chemistry and Pharmaceutical Engineering, Henan University of Science and Technology, Luoyang 471023, China. shriib@126.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
authors.affiliation: College of Chemistry and Pharmaceutical Engineering, Henan University of Science and Technology, Luoyang 471023, China. jhltl2003@126.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
mesh_terms: D006868:Hydrolysis; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D012503:Saponins / Q000737:chemistry*
chemical_list: D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D
keywords: acid hydrolysis; degradation product; saikosaponin A