title: A Guide to Fluorescent Protein FRET Pairs.
abstract: Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most commonly used as both donor and acceptor fluorophores in FRET biosensors, especially since FPs are genetically encodable and live-cell compatible. In this review, we will provide an overview of methods to measure FRET changes in biological contexts, discuss the palette of FP FRET pairs developed and their relative strengths and weaknesses, and note important factors to consider when using FPs for FRET studies.
authors.affiliation: Medical Scientist Training Program, University of California, Los Angeles, CA 90095, USA. BBajar@mednet.ucla.edu.
authors.forename: Bryce T
authors.initials: BT
authors.lastname: Bajar
authors.affiliation: Harvard College, Cambridge, MA 02138, USA. emilyswang@college.harvard.edu.
authors.forename: Emily S
authors.initials: ES
authors.lastname: Wang
authors.affiliation: Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. shu.zhang@siat.ac.cn.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Departments of Bioengineering and Neurobiology, Stanford University, CA 94305, USA. mzlin@stanford.edu.
authors.forename: Michael Z
authors.initials: MZ
authors.lastname: Lin
authors.affiliation: Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. jun.chu@siat.ac.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Chu
mesh_terms: D003116:Color; D031541:Fluorescence Resonance Energy Transfer; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D008164:Luminescent Proteins / Q000378:metabolism*; D055503:Protein Multimerization; D055550:Protein Stability / Q000528:radiation effects
chemical_list: D008164:Luminescent Proteins
keywords: biosensors; fluorescence resonance energy transfer (FRET); fluorescent proteins