title: Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiationin vitro.
abstract: The preadipocyte differentiation biological process involves a cascade of transcriptional events that culminates in the expression of peroxisome proliferator-activated receptor (PPAR) γ. The differentiation cocktail [insulin (INS), dexamethasone (DEX) and isobutylmethylxanthine (IBMX)] can induce preadipocyte differentiation in mammals, but it is insufficient for chicken (Gallus gallus) adipogenesis. Oleate can induce chicken preadipocyte differentiation, but these differentiated preadipocytes may not be fully functional. The objective of the current study was to evaluate whether chicken preadipocytes can be induced to mature adipocytes by a novel induction method using differentiation cocktail supplemented with PPARγ agonist(s). Chicken preadipocytes cultured in cocktail supplemented with rosiglitazone or troglitazone resulted in a marked increase in lipid droplet accumulation (P<0.05), glycerol-3-phosphate dehydrogenase (GPDH) activity (P<0.05), mRNA expression level of adipocyte fatty acid-binding protein (aP2;P<0.05), G0/G1switch gene 2 (G0S2;P<0.05) and lipolysis (P<0.05). In addition, supplementation of the cocktail with rosiglitazone promoted PPARγ mRNA expression (P<0.05). In conclusion, our data indicated that chicken preadipocytes can be induced to mature adipocytes using differentiation cocktail supplemented with rosiglitazone. The results of the present study provide a novel induction method forin vitrochicken preadipocyte differentiation.
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, PR China.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, PR China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Bohan
authors.initials: B
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, PR China.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, PR China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Mengqi
authors.initials: M
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, PR China.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, PR China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Songsong
authors.initials: S
authors.lastname: Xu
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, PR China.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, PR China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Xinyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, PR China.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, PR China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, PR China.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, PR China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, PR China.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, PR China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Leng
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, PR China lihui@neau.edu.cn.|Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030, PR China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
keywords: chicken; cocktail; differentiation; inducer; peroxisome proliferator-activated receptor γ (PPARγ); preadipocyte