title: Screening of Cyanobacterial Peptide Toxin, Microcystins in Hyperscum Water Samples from an Inland Sub Saharan Drinking Freshwater Reservoir.
abstract: A study which probed the occurrence and quantitative variations hepatotoxic microcystin in a Sub Saharan drinking freshwater reservoir was carried out between November 2014 and March 2015. Results reveal the presence of MCYST-YR, MCYST-LR, MCYST-RR, MCYST-LA and MCYST-LF variants either in cells collected directly from bloom or toxic isolates cultured under laboratory conditions. Two minor microcystin congeners (MCYST-(H4)YR) and (D-Asp3, Dha7) MCYST-RR) were identified, but not quantified. Variants dominance were in the order MCYST-LR > MCYST-RR > MCYST-YR > MCYST-LA > MCYST-LF across sampling sites. Maximum and minimum concentrations of quantified MCYSTs congeners were (489.25, 50.95 µg toxin/g DW), (98.92, 9.11 µg toxin/g DW), (140.25, 12.07 µg toxin/g DW), (56.99, 6.20 µg toxin/g DW) and (50.46, 3.65 µg toxin/g DW) for MCYST-LR, MCYST-YR, MCYST-RR, MCYST-LA and MCYST-LF, respectively. Analysis of variance (ANOVA) revealed there was a high significant difference between mean microcystin concentrations across sampling sites (p < 0.05).
authors.affiliation: Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg "Doornfontein Campus", Doornfontein, Johannesburg, 2028, South Africa. eguzoziekennedy@yahoo.co.uk.
authors.forename: Kennedy Uchenna
authors.initials: KU
authors.lastname: Eguzozie
authors.affiliation: Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg "Doornfontein Campus", Doornfontein, Johannesburg, 2028, South Africa.
authors.forename: Vuyo
authors.initials: V
authors.lastname: Mavumengwana
authors.affiliation: Department of Applied Chemistry, Faculty of Science, University of Johannesburg "Doornfontein Campus", Doornfontein, Johannesburg, 2028, South Africa.
authors.forename: Duduzile
authors.initials: D
authors.lastname: Nkosi
authors.affiliation: Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg "Doornfontein Campus", Doornfontein, Johannesburg, 2028, South Africa.
authors.forename: Eugenie
authors.initials: E
authors.lastname: Kayitesi
authors.affiliation: Department of Environmental Sciences, College of Agriculture and Environmental Sciences, University of South Africa "Florida Campus", Florida, Johannesburg, 1709, South Africa.
authors.forename: Emmanuella Chioma
authors.initials: EC
authors.lastname: Nnabuo-Eguzozie
mesh_terms: D017954:Africa South of the Sahara; D001427:Bacterial Toxins / Q000032:analysis*; D000087522:Cyanobacteria Toxins; D005618:Fresh Water / Q000032:analysis; D008387:Marine Toxins / Q000032:analysis*; D052998:Microcystins / Q000032:analysis*
chemical_list: D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR
keywords: Bioaccumulation; Cyanotoxin; Hazardous cyanobacterial bloom; Hepatotoxin; Solid phase extraction