title: Gene expression profiling of CD8+T cells induced by ovarian cancer cells suggests a possible mechanism for CD8+Treg cell production.
abstract: OBJECTIVE
  The aim of this study was to investigate a possible mechanism of CD8+regulatory T-cell (Treg) production in an ovarian cancer (OC) microenvironment.
  
  
  METHODS
  Agilent microarray was used to detect changes in gene expression between CD8+T cells cultured with and without the SKOV3 ovarian adenocarcinoma cell line. QRT-PCR was performed to determine glycolysis gene expression in CD8+T cells from a transwell culturing system and OC patients. We also detected protein levels of glycolysis-related genes using Western blot analysis.
  
  
  RESULTS
  Comparing gene expression profiles revealed significant differences in expression levels of 1420 genes, of which 246 were up-regulated and 1174 were down-regulated. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that biological processes altered in CD8+Treg are particularly associated with energy metabolism. CD8+Treg cells induced by co-culture with SKOV3 had lower glycolysis gene expression compared to CD8+T cells cultured alone. Glycolysis gene expression was also decreased in the CD8+T cells of OC patients.
  
  
  CONCLUSIONS
  These findings provide a comprehensive bioinformatics analysis of DEGs in CD8+T cells cultured with and without SKOV3 and suggests that metabolic processes may be a possible mechanism for CD8+Treg induction.
authors.affiliation: Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing, China.|National Key Clinical Department of Laboratory Medicine, 210029, Nanjing, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing, China.|National Key Clinical Department of Laboratory Medicine, 210029, Nanjing, China.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Lou
authors.affiliation: Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing, China.|National Key Clinical Department of Laboratory Medicine, 210029, Nanjing, China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing, China.|National Key Clinical Department of Laboratory Medicine, 210029, Nanjing, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing, China.|National Key Clinical Department of Laboratory Medicine, 210029, Nanjing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing, China.|National Key Clinical Department of Laboratory Medicine, 210029, Nanjing, China.
authors.forename: Ruihong
authors.initials: R
authors.lastname: Sun
authors.affiliation: Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing, China.|National Key Clinical Department of Laboratory Medicine, 210029, Nanjing, China.
authors.forename: Peijun
authors.initials: P
authors.lastname: Huang
authors.affiliation: Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing, China.|National Key Clinical Department of Laboratory Medicine, 210029, Nanjing, China.
authors.forename: Shiyang
authors.initials: S
authors.lastname: Pan
authors.affiliation: Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing, China. wangfang@njmu.edu.cn.|National Key Clinical Department of Laboratory Medicine, 210029, Nanjing, China. wangfang@njmu.edu.cn.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D004734:Energy Metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006019:Glycolysis; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010053:Ovary / Q000378:metabolism / Q000473:pathology*; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism* / Q000473:pathology