title: Anti-Inflammatory Effects of Liriope platyphylla in LPS-Stimulated Macrophages and Endotoxemic Mice.
abstract: In the present study, the anti-inflammatory and antisepticemic activities of a water extract of Liriope platyphylla (LP) were investigated. We first estimated the scavenging activity of DPPH and the hydroxyl radical and total phenolic contents of LP. Results indicated that LP, a rich source of phenolic compounds, showed a remarkable radical scavenging capacity. A MTT assay showed that LP treatment did not affect the toxicity against the RAW 264.7 macrophage cells, up to the concentration of 500[Formula: see text][Formula: see text]g/mL. Treatment of LP significantly attenuated the production of inflammatory mediators, such as nitric oxide (NO), interleukin-6 (IL-6), tumor-necrosis factor (TNF)-[Formula: see text] and prostaglandin (PG)E2in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages cells. Moreover, LP contributed to the down-regulation of inducible NO synthase (iNOS) and TNF-[Formula: see text] mRNA expression, as well as cyclooxygenase-2 (COX-2) protein expression. A western blotting assay further showed that LP inhibited activation of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-[Formula: see text]B. In an animal experiment using an LPS-induced septicemia model in C57BL/6 mice, oral administration of LP (40[Formula: see text]mg/kg body weight) markedly reduced the level of TNF-[Formula: see text] and IL-6 in serum and protected against LPS-induced lethal shock in mice. Taken together, the results of treatments of LP on inhibited LPS-induced inflammatory responses in both in vitro and in vivo models and indicate it may be a promising neutraceutical or medicinal agent to prevent or cure inflammation-related disease.
authors.affiliation: * Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Min-Jee
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: † Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-632, Korea.
authors.forename: Yung-Choon
authors.initials: YC
authors.lastname: Yoo
authors.affiliation: * Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Nak-Yun
authors.initials: NY
authors.lastname: Sung
authors.affiliation: † Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-632, Korea.
authors.forename: Julim
authors.initials: J
authors.lastname: Lee
authors.affiliation: † Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-632, Korea.
authors.forename: Seok-Rae
authors.initials: SR
authors.lastname: Park
authors.affiliation: * Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Shon
authors.affiliation: * Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Bo Dam
authors.initials: BD
authors.lastname: Lee
authors.affiliation: * Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Mee Ree
authors.initials: MR
authors.lastname: Kim
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000188:drug therapy* / Q000378:metabolism; D016166:Free Radical Scavengers / Q000032:analysis / Q000302:isolation & purification; D018836:Inflammation Mediators / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects*; D031424:Liriope Plant / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use*; D000067996:RAW 264.7 Cells; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D016166:Free Radical Scavengers; D018836:Inflammation Mediators; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010636:Phenols; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha
keywords: Anti-Inflammation; Anti-Septicemia; Liriope platyphylla; MAPK; NF-κB