title: ZNF606 interacts with Sox9 to regulate chondrocyte differentiation.
abstract: SRY-related high-mobility-group box 9 (Sox9) gene is a transcription factor that plays an essential role in chondrocyte differentiation and cartilage formation. In this study, we identified the transcriptional factor ZNF606 as an interacting partner for Sox9 in cells. We further demonstrated that overexpression of ZNF606 inhibited the transcriptional activity of Sox9, while knockdown of ZNF606 increased Sox9-mediated transcription. Chromatin immunoprecipitation analysis revealed that ZNF606 prevents Sox9 binding to the enhancers of its target gene col2a1. Importantly, the interaction between ZNF606 and Sox9 was decreased during chondrocyte differentiation. Consistent with these findings, ZNF606 inhibited chondrocyte differentiation. Thus, our results demonstrate that ZNF606 acts as a novel Sox9 co-regulator that inhibits Sox9-mediated chondrocyte differentiation.
authors.affiliation: Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China.
authors.forename: Zhenhai
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China.
authors.forename: Honggui
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China.
authors.forename: Yunjia
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China.
authors.forename: Longjie
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, China. Electronic address: zhq9996@163.com.
authors.forename: Hongqi
authors.initials: H
authors.lastname: Zhang
mesh_terms: D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism*; D020219:Chondrogenesis / Q000235:genetics / Q000502:physiology*; D024043:Collagen Type II / Q000235:genetics; D004742:Enhancer Elements, Genetic; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D061986:MCF-7 Cells; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D055758:SOX9 Transcription Factor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C499177:COL2A1 protein, human; D024043:Collagen Type II; D012097:Repressor Proteins; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C504030:ZNF606 protein, human
keywords: Chondrocyte differentiation; Sox9; ZNF606