title: Isolation of autologous adipose tissue-derived mesenchymal stem cells for bone repair.
abstract: Adipose tissue represents an abundant and accessible source of adult stem cells that can differentiate into cells and tissues of mesodermal origin, including osteogenic cells.
  
  
  
  This paper describes the procedure to obtain a 5-cm3saline sample, containing the adipose-derived stem cells (ASCs) pellet, starting from lipoaspirate obtained from a conventional abdominal liposuction.
  
  
  
  A mean of 2.5×106cells is isolated for each procedure; 35% (875000) of these are CD34+/CD45- cells, which express a subset of both positive (CD10, CD13, CD44, CD59, CD73, CD90, HLAABC) and negative (CD33, CD39, CD102, CD106, CD146, HLADR) cell-associated surface antigens, characterizing them as ASCs.
  
  
  
  This procedure is easy, effective, economic and safe. It allows the harvesting of a significant number of ASCs that are ready for one-step bony regenerative surgical procedures.
authors.affiliation: Department of surgical sciences, plastic surgery section, university of Parma, via Gramsci 14, 43100 Parma, Italy. Electronic address: edoardo.raposio@unipr.it.
authors.forename: E
authors.initials: E
authors.lastname: Raposio
authors.affiliation: Department of clinical and experimental medicine, division of hematology, Parma university hospital, via Gramsci 14, 43100 Parma, Italy. Electronic address: sBonomimi@ao.pr.it.
authors.forename: S
authors.initials: S
authors.lastname: Bonomini
authors.affiliation: Department of surgical sciences, orthopedic clinic, Parma university hospital, via Gramsci 14, 43100 Parma, Italy. Electronic address: filippo.calderazzi@icloud.com.
authors.forename: F
authors.initials: F
authors.lastname: Calderazzi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D015187:Lipectomy; D059630:Mesenchymal Stem Cells / Q000166:cytology*
keywords: Adipose-derived stem cells; Bone and cartilage; Cell therapy