title: MiR-203 promotes the growth and migration of ovarian cancer cells by enhancing glycolytic pathway.
abstract: MicroRNAs (miRNAs) play an important role in the tumorigenesis of ovarian cancer. Previously, we have reported the dysregulation of miR-203 in the ovarian cancer tissues. However, the biological functions and molecular mechanisms of miR-203 in ovarian cancer remain unknown. Here, we showed that the expression of miR-203 was increased in ovarian cancer tissues compared with the adjacent non-cancerous tissues and the transcription of miR-203 was inhibited by P53. Forced expression of miR-203 in ovarian cancer promoted cell growth and migration, while depletion of miR-203 inhibited the growth and migration of ovarian cancer cells. In addition, miR-203 promoted the metastasis of ovarian cancer cells in vivo and shorted the survival of the nude mice. Mechanically, miR-203 targeted the 3'-UTR of pyruvate dehydrogenase B (PDHB) and increased the consumption of glucose and the production of lactate. Overexpression of PDHB abolished the oncogenic effects of miR-203 on the growth of ovarian cancer cells. Together, our data suggested the oncogenic roles of miR-203 in ovarian cancer by promoting glycolysis, and miR-203 might be a therapeutic target for ovarian cancer.
authors.affiliation: Maternal and Child Health Hospital of Hainan Province, 15th South of Longkun Road, Haikou, Hainan Province, 570206, People's Republic of China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Xiaohong
authors.affiliation: Maternal and Child Health Hospital of Hainan Province, 15th South of Longkun Road, Haikou, Hainan Province, 570206, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Lichun
authors.affiliation: Department of Pathology, The Affiliated Hospital of Hainan Medical University, Haikou, Hainan Province, 571101, China.
authors.forename: Xie
authors.initials: X
authors.lastname: Na
authors.affiliation: Department of Gastrointestinal Surgery, Hainan Province People's Hospital, Haikou, Hainan Province, 570206, China.
authors.forename: Zou
authors.initials: Z
authors.lastname: Kejian
authors.affiliation: Department of Clinical Pathology, Hainan Province Hospital, Haikou, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiaolan
authors.affiliation: Maternal and Child Health Hospital of Hainan Province, 15th South of Longkun Road, Haikou, Hainan Province, 570206, People's Republic of China. wangshaosheng1012@163.com.
authors.forename: Wang
authors.initials: W
authors.lastname: Shaosheng
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D063646:Carcinogenesis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D019344:Lactic Acid / Q000096:biosynthesis; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology*; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; D011768:Pyruvate Dehydrogenase Complex; D016159:Tumor Suppressor Protein p53; D019344:Lactic Acid; D005947:Glucose
keywords: Cell growth and migration; Glycolysis; MiR-203; Ovarian cancer; PDHB