title: Simultaneous aerobic denitrification and Cr(VI) reduction by Pseudomonas brassicacearum LZ-4 in wastewater.
abstract: Inorganic nitrogen and heavy metals pervasively co-exist in industrial and domestic wastewaters. In this work, Pseudomonas brassicacearum LZ-4 was tested for the simultaneous reduction of Cr(VI) and nitrate. Nitrate was found to be the best inorganic nitrogen source for strain LZ-4, and could promote Cr(VI) reduction. Cr(VI) had a low degree of inhibition on denitrification, and even 50mgL-1Cr(VI) did not inhibit reduction of 100mgL-1NO3--N. The capability of simultaneous reduction of Cr(VI) and nitrate was illustrated by the reductase genes contained in the LZ-4 genome. Application in a batch membrane bioreactor showed that the immobilized strain LZ-4 could remove over 95% of 500mgL-1NO3--N, 80% of 10mgL-1Cr(VI), and 96% of 5000mgL-1COD in each batch of 46days. In summary, the strain LZ-4 is an ideal candidate for remediation of co-contaminants.
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Science, Lanzhou University, Tianshuinanlu #222, Lanzhou, Gansu 730000, PR China; Gansu Academy of Membrane Science and Technology, Duanjiatanlu #1272, Lanzhou, Gansu 730020, PR China; School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou, Jiangsu 221116, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Yu
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Science, Lanzhou University, Tianshuinanlu #222, Lanzhou, Gansu 730000, PR China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Science, Lanzhou University, Tianshuinanlu #222, Lanzhou, Gansu 730000, PR China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Huang
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Science, Lanzhou University, Tianshuinanlu #222, Lanzhou, Gansu 730000, PR China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Shi
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Science, Lanzhou University, Tianshuinanlu #222, Lanzhou, Gansu 730000, PR China.
authors.forename: Kejia
authors.initials: K
authors.lastname: Wu
authors.affiliation: Gansu Academy of Membrane Science and Technology, Duanjiatanlu #1272, Lanzhou, Gansu 730020, PR China.
authors.forename: Pengyun
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Gansu Academy of Membrane Science and Technology, Duanjiatanlu #1272, Lanzhou, Gansu 730020, PR China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Lv
authors.affiliation: PetroChina Lanzhou Petrochemical Company, Yumenjie #10, Lanzhou, Gansu 730060, PR China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou, Jiangsu 221116, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: He
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Science, Lanzhou University, Tianshuinanlu #222, Lanzhou, Gansu 730000, PR China.
authors.forename: Pu
authors.initials: P
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Science, Lanzhou University, Tianshuinanlu #222, Lanzhou, Gansu 730000, PR China. Electronic address: xkli@lzu.edu.cn.
authors.forename: Xiangkai
authors.initials: X
authors.lastname: Li
mesh_terms: D019149:Bioreactors; D002857:Chromium / Q000737:chemistry*; D058440:Denitrification; D019216:Metals, Heavy; D009566:Nitrates / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D011549:Pseudomonas; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology; D018508:Water Purification / Q000379:methods*
chemical_list: D002857:Chromium; D019216:Metals, Heavy; D009566:Nitrates; D009584:Nitrogen; D062065:Wastewater; C074702:chromium hexavalent ion
keywords: Co-contaminants; Cr(VI) reduction; Denitrification; Pseudomonas