title: Overexpression of a glyoxalase gene, OsGly I, improves abiotic stress tolerance and grain yield in rice (Oryza sativa L.).
abstract: Glyoxalase I (Gly I) is a component of the glyoxalase system which is involved in the detoxification of methylglyoxal, a byproduct of glycolysis. In the present study, a gene of rice (Oryza sativa L., cv. Nipponbare) encoding Gly I was cloned and characterized. The quantitative real-time PCR analysis indicated that rice Gly I (OsGly I) was ubiquitously expressed in root, stem, leaf, leaf sheath and spikelet with varying abundance. OsGly I was markedly upregulated in response to NaCl, ZnCl2and mannitol in rice seedlings. For further functional investigation, OsGly I was overexpressed in rice using Agrobacterium-mediated transformation. Transgenic rice lines exhibited increased glyoxalase enzyme activity, decreased methylglyoxal level and improved tolerance to NaCl, ZnCl2and mannitol compared to wild-type plants. Enhancement of stress tolerance in transgenic lines was associated with reduction of malondialdehyde content which was derived from cellular lipid peroxidation. In addition, the OsGly I-overexpression transgenic plants performed higher seed setting rate and yield. Collectively, these results indicate the potential of bioengineering the Gly I gene in crops.
authors.affiliation: College of Life Science, Chongqing University, Chongqing 400030, China.
authors.forename: Zhengming
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: College of Life Science, Chongqing University, Chongqing 400030, China.
authors.forename: Fangjie
authors.initials: F
authors.lastname: Xiong
authors.affiliation: College of Life Science, Chongqing University, Chongqing 400030, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Life Science, Chongqing University, Chongqing 400030, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Gong
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Sichuan Academy of Agricultural Sciences, Rice and Sorghum Research Institute, Luzhou Branch of National Rice Improvement Center, Ministry of Agriculture, Deyang 618000, China.
authors.forename: Juntao
authors.initials: J
authors.lastname: Luo
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Sichuan Academy of Agricultural Sciences, Rice and Sorghum Research Institute, Luzhou Branch of National Rice Improvement Center, Ministry of Agriculture, Deyang 618000, China.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Sichuan Academy of Agricultural Sciences, Rice and Sorghum Research Institute, Luzhou Branch of National Rice Improvement Center, Ministry of Agriculture, Deyang 618000, China.
authors.forename: Haochi
authors.initials: H
authors.lastname: Kuang
authors.affiliation: Key Laboratory of Southwest Rice Biology and Genetic Breeding, Sichuan Academy of Agricultural Sciences, Rice and Sorghum Research Institute, Luzhou Branch of National Rice Improvement Center, Ministry of Agriculture, Deyang 618000, China.
authors.forename: Bijun
authors.initials: B
authors.lastname: Gao
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address: niu_xiangli@163.com.
authors.forename: Xiangli
authors.initials: X
authors.lastname: Niu
authors.affiliation: College of Life Science, Chongqing University, Chongqing 400030, China; School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China; Ministry of Education Key Laboratory for Bio-resource and Eco-environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China. Electronic address: liu_ys@scu.edu.cn.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D002712:Chlorides / Q000494:pharmacology; D002523:Edible Grain / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D056626:Inflorescence / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D007791:Lactoylglutathione Lyase / Q000235:genetics* / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011765:Pyruvaldehyde / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects; D017967:Zinc Compounds / Q000494:pharmacology
chemical_list: D002712:Chlorides; D010940:Plant Proteins; D017967:Zinc Compounds; D008353:Mannitol; D012965:Sodium Chloride; D008315:Malondialdehyde; D011765:Pyruvaldehyde; C016837:zinc chloride; D007791:Lactoylglutathione Lyase
keywords: Glyoxalase I; Grain yield; Rice; Stress tolerance