title: Effect of tumor necrosis factor-induced protein 8 on T-cell-mediated immunity in mice after thermal injury.
abstract: Tumor necrosis factor-induced protein 8(TNFAIP8), the first identified member of the TNFAIP8 family, shares considerable sequence homology with members of this family. It is expressed in a wide variety of human normal tissues, with relatively higher levels in lymphoid tissues and placenta. The present study was designed to examine the effect of TNFAIP8 on T-cell-mediated immunity secondary to burn injury. Sixty male mice were randomly divided into four groups as follows: sham burn group, burn group, burn with TNFAIP8-siRNA transfection group, and burn with negative control transfection group, and they were sacrificed at designated time points. CD4+ T cells were isolated using MACS microbeads. T-Cell proliferation was analyzed with MTT assay, and IL-2, soluble IL-2R, IL-4, interferon-γ (IFN-γ) were determined with enzyme-linked immunosorbent assay kits. It was found that CD4+ T lymphocyte proliferative activity was significantly down-regulated when TNFAIP8 gene was silenced by siRNA in mice at 24 h post burn. Down-regulation of TNFAIP8 can significantly decrease expression levels of IL-2 and soluble IL-2R at 24 h after thermal injury. These results demonstrated that TNFAIP8 appeared to be involved in the immune regulation of CD4+ T lymphocytes, and the decreased expression of TNFAIP8 could affect T lymphocyte functions after thermal injury.
authors.affiliation: Department of Pediatrics, Oilu Hospital, Shandong University, Jinan, P.R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai, P.R. China.
authors.forename: D H
authors.initials: DH
authors.lastname: Wan
authors.affiliation: Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai, P.R. China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai, P.R. China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Gao
authors.affiliation: Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai, P.R. China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Xu
authors.affiliation: Department of Pediatrics, Oilu Hospital, Shandong University, Jinan, P.R. China.
authors.forename: X L
authors.initials: XL
authors.lastname: Ju
authors.affiliation: Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai, P.R. China.
authors.forename: A M
authors.initials: AM
authors.lastname: Li
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D002056:Burns / Q000235:genetics / Q000276:immunology*; D018791:CD4 Lymphocyte Count; D015536:Down-Regulation; D007111:Immunity, Cellular / Q000235:genetics*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000235:genetics; D011897:Random Allocation; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism; D014162:Transfection
chemical_list: D051017:Apoptosis Regulatory Proteins; D007376:Interleukin-2; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D015375:Receptors, Interleukin-2; C550729:TNFAIP8 protein, mouse; D007371:Interferon-gamma