title: Proximal Tubule CD73 Is Critical in Renal Ischemia-Reperfusion Injury Protection.
abstract: CD73-derived adenosine plays an anti-inflammatory role in various organs. However, its role in renal ischemia-reperfusion injury (IRI) is controversial. We targeted CD73 mutant mice to determine the function of CD73 expressed by various renal cell types under mild IRI conditions. Mice with CD73 deletion in proximal tubules exhibited exacerbated IRI, comparable with that ofCD73-/-mice compared with WT mice. Mice with CD73 deletions in other cell types, including cortical type 1 fibroblast-like cells, mesangial cells, macrophages, and dendritic cells, showed small or no increases in injury above control mice when subjected to threshold levels of ischemia. Results from adoptive transfer experiments between WT andCD73-/-mice and pharmacologic studies modulating enzymatic activity of CD73 and extracellular adenosine levels supported a critical role of adenosine generated by proximal tubule CD73 expression in abrogating IRI. Renal adenosine levels were lower before and after ischemia in CD73-deficient mice. However, reduction in total acid-extractable renal adenosine levels was inadequate to explain the marked difference in kidney injury in these CD73-deficient mice. Furthermore, CD73 inhibition and enzyme replacement studies showed no change in total kidney adenosine levels in treated mice compared with vehicle-treated controls. Protection from IRI in neutrophil-depleted WT recipients was sustained by repopulation with bone marrow neutrophils from WT mice but not by those lacking adenosine 2a receptors (fromAdora2a-/-mice). These data support the thesis that local adenosine generated by cells at the injury site is critical for protection from IRI through bone marrow-derived adenosine 2a receptors.
authors.affiliation: Division of Nephrology and sjs5c@Virginia.edu.|Center for Immunity, Inflammation, and Regenerative Medicine, Department of Medicine and.
authors.forename: Sun-Sang J
authors.initials: SJ
authors.lastname: Sung
authors.affiliation: Division of Nephrology and.|Center for Immunity, Inflammation, and Regenerative Medicine, Department of Medicine and.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Division of Nephrology and.|Center for Immunity, Inflammation, and Regenerative Medicine, Department of Medicine and.
authors.forename: Liping
authors.initials: L
authors.lastname: Huang
authors.affiliation: Division of Nephrology and.|Center for Immunity, Inflammation, and Regenerative Medicine, Department of Medicine and.
authors.forename: Jessica
authors.initials: J
authors.lastname: Lawler
authors.affiliation: Division of Nephrology and.|Center for Immunity, Inflammation, and Regenerative Medicine, Department of Medicine and.
authors.forename: Hong
authors.initials: H
authors.lastname: Ye
authors.affiliation: Center for Immunity, Inflammation, and Regenerative Medicine, Department of Medicine and.|Departments of Pharmacology and.
authors.forename: Diane L
authors.initials: DL
authors.lastname: Rosin
authors.affiliation: Division of Nephrology and.|Center for Immunity, Inflammation, and Regenerative Medicine, Department of Medicine and.
authors.forename: Issah S
authors.initials: IS
authors.lastname: Vincent
authors.affiliation: Division of Nephrology and.|Center for Immunity, Inflammation, and Regenerative Medicine, Department of Medicine and.
authors.forename: Thu H
authors.initials: TH
authors.lastname: Le
authors.affiliation: Cell Biology, University of Virginia, Charlottesville, Virginia; and.
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Molecular Cardiology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Görldt
authors.affiliation: Department of Molecular Cardiology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schrader
authors.affiliation: Division of Nephrology and.|Center for Immunity, Inflammation, and Regenerative Medicine, Department of Medicine and.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Okusa
mesh_terms: D015720:5'-Nucleotidase / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D007668:Kidney / Q000098:blood supply*; D007687:Kidney Tubules, Proximal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015427:Reperfusion Injury / Q000209:etiology*
chemical_list: D015720:5'-Nucleotidase
keywords: Immunology and pathology; acute renal failure; creatinine; ischemia-reperfusion; renal proximal tubule cell