title: Oxidation Stability of O/W Emulsion Prepared with Linolenic Acid Enriched Diacylglycerol.
abstract: The sn-1,3-regiospecific Rhizomucor miehei lipase (Lipozyme RM IM) was employed to produce structured diacylglycerol (SL-DAG), which contained 67.3 mol% DAG with 27.2 area% of C18:3. To investigate the oxidative stability of the SL-DAG in emulsion form, 5% oil-in-water (O/W) emulsions were prepared with 200 and 400 ppm sinapic acid. It was shown that the hydroperoxide values of the control (without any antioxidant) was the highest (117.7 meq/L) on day 43 of storage and thereafter the value decreased. However, the emulsions with 200 and 400 ppm sinapic acid resulted in slow oxidation degree until day 64 of storage (30.3 and 7.3 meq/L, respectively). Aldehyde measurements for the 200 ppm sinapic acid emulsion (12.8 mmol/mol) and the 400 ppm sinapic acid emulsion (7.5 mmol/mol) also showed better oxidative stability than that for the 200 ppm catechin emulsion (27.4 mmol/mol) and the control (52.7 mmol/mol). Although the SL-DAG in the emulsions contains high levels of polyunsaturated fatty acids, the degree of oxidation in the emulsions can be reduced when sinapic acid is used as an antioxidant.
authors.affiliation: Dept. of Food Science and Technology, Chungnam National Univ, Daejeon, 305-764, South Korea.
authors.forename: Jung-Ah
authors.initials: JA
authors.lastname: Shin
authors.affiliation: Chungcheongnam-do Health and Environmental Research Inst, Daejeon, 300-801, South Korea.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Lee
authors.affiliation: Dept. of Food Science and Technology, Chungnam National Univ, Daejeon, 305-764, South Korea. ktlee@cnu.ac.kr.
authors.forename: Ki-Teak
authors.initials: KT
authors.lastname: Lee
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D029741:Brassica rapa / Q000737:chemistry; D002392:Catechin / Q000494:pharmacology; D003373:Coumaric Acids / Q000494:pharmacology*; D004075:Diglycerides / Q000096:biosynthesis / Q000378:metabolism*; D004655:Emulsions; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000494:pharmacology*; D020103:Rhizomucor / Q000201:enzymology; D014867:Water; D017962:alpha-Linolenic Acid / Q000378:metabolism*
chemical_list: C011439:1,2-diacylglycerol; D000975:Antioxidants; D003373:Coumaric Acids; D004075:Diglycerides; D004655:Emulsions; D010936:Plant Extracts; D014867:Water; D017962:alpha-Linolenic Acid; C073734:sinapinic acid; D002392:Catechin; D006861:Hydrogen Peroxide; C093666:Lipozyme; D008049:Lipase
keywords: diacylglycerol; linolenic acid; oil-in-water emulsion; oxidative stability; sinapic acid