title: Regenerative Characteristics of Apical Papilla-derived Cells from Immature Teeth with Pulpal and Periapical Pathosis.
abstract: BACKGROUND
  The purpose of this study was to ascertain the regenerative characteristics of apical papilla-derived cells (APDCs) from immature teeth with pulpal and periapical pathosis and thus to provide proof-of-principle evidence for further regenerative endodontic research.
  
  
  METHODS
  Pulpal and periapical pathosis was induced in immature permanent double-rooted premolars of beagles, which were randomly assigned to experimental treatment groups: group AO (n = 14), pulp disruption and access left open; group PS (n = 14), supragingival plaque suspension-soaked cotton pellet was introduced, and access was sealed; and control (n = 7), untreated. The teeth were extracted at 2- and 4-week periods after experimental treatments. APDCs were cultured from the extracted teeth, and their cellular proliferation, differentiation characteristics, and stemness were assessed. The data were statistically analyzed.
  
  
  RESULTS
  After 4 weeks of intentional pulpal and periapical pathosis induction period, all teeth in group PS showed features of apical periodontitis with necrotic pulp, and their APDCs showed significantly increased proliferation rate and osteogenic/odontogenic differentiation capabilities (P < .05). The stemness was maintained in all APDCs, although the stem cell population was smaller in group PS at 2-week period when the inflammatory responses were most fulminant (P < .05).
  
  
  CONCLUSIONS
  The APDCs from immature teeth retained the regenerative characteristics with the differences according to their pulpal and periapical pathosis. The results of this study partly provide the evidence for regenerative endodontic research.
authors.affiliation: Department of Conservative Dentistry, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: Yeon-Jee
authors.initials: YJ
authors.lastname: Yoo
authors.affiliation: Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea; Department of BK21 Plus Program, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: Joung-Hwan
authors.initials: JH
authors.lastname: Oh
authors.affiliation: Department of Conservative Dentistry, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: WooCheol
authors.initials: W
authors.lastname: Lee
authors.affiliation: Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea; Department of BK21 Plus Program, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea; Department of Pharmacology and Dental Therapeutics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea. Electronic address: kmwoo@snu.ac.kr.
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Woo
mesh_terms: D000818:Animals; D001641:Bicuspid / Q000473:pathology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D003771:Dental Papilla / Q000473:pathology / Q000503:physiopathology*; D003782:Dental Pulp / Q000473:pathology / Q000503:physiopathology; D003790:Dental Pulp Necrosis / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D004285:Dogs; D023421:Models, Animal; D009805:Odontogenesis; D010012:Osteogenesis; D010485:Periapical Periodontitis / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D010486:Periapical Tissue / Q000473:pathology / Q000503:physiopathology*; D012038:Regeneration / Q000502:physiology*
chemical_list: D016207:Cytokines
keywords: Apical papilla-derived cells; endodontic animal model; immature tooth; regenerative endodontic procedure