title: The First Siphoviridae Family Bacteriophages Infecting Bordetella bronchiseptica Isolated from Environment.
abstract: Bordetella bronchiseptica is a well-known etiological agent of kennel cough in dogs and cats and one of the two causative agents of atrophic rhinitis, a serious swine disease. The aim of the study was to isolate B. bronchiseptica bacteriophages from environmental samples for the first time. A total of 29 phages from 65 water samples were isolated using the strain ATCC 10580 as a host. The lytic spectra of the phages were examined at 25 and 37 °C, using 12 strains of B. bronchiseptica. All phages were able to plaque on 25.0 % to 41.7 % of the strains. The selected phages showed similar morphology (Siphoviridae, morphotype B2), but variation of RFLP patterns and efficacy of plating on various strains. The partial genome sequence of phage vB_BbrS_CN1 showed its similarity to phages from genus Yuavirus. Using PCR, it was confirmed that the phages do not originate from the host strain, and environmental origin was additionally confirmed by the analysis of host genome sequence in silico and plating heated and unheated samples in parallel. Accordingly, this is the first isolation of B. bronchiseptica phages from environment and the first isolation and characterization of phages of B. bronchiseptica belonging to family Siphoviridae.
authors.affiliation: Faculty of Sciences, Department of Biology and Ecology, University of Novi Sad, Trg Dositeja Obradovica 3, 21000, Novi Sad, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Petrovic
authors.affiliation: Biotechnical Faculty, Department of Biology, University of Ljubljana, Vecna pot 111, Ljubljana, 1000, Slovenia.
authors.forename: Rok
authors.initials: R
authors.lastname: Kostanjsek
authors.affiliation: Department of Biotechnology, University of Szeged, Temesvari krt. 62, H-6726, Szeged, Hungary.
authors.forename: Gabor
authors.initials: G
authors.lastname: Rakhely
authors.affiliation: Faculty of Sciences, Department of Biology and Ecology, University of Novi Sad, Trg Dositeja Obradovica 3, 21000, Novi Sad, Serbia. petar.knezevic@dbe.uns.ac.rs.
authors.forename: Petar
authors.identifier: 0000-0003-0353-3892
authors.initials: P
authors.lastname: Knezevic
mesh_terms: D001435:Bacteriophages / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D001483:Base Sequence; D016950:Bordetella bronchiseptica / Q000235:genetics / Q000821:virology*; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004777:Environment; D005814:Genes, Viral; D016679:Genome, Viral; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017906:Siphoviridae / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D062065:Wastewater / Q000821:virology; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D062065:Wastewater
keywords: Bacteriophage; Bordetella bronchiseptica; Isolation; Siphoviridae