title: An enzyme immunoassay for determining epidermal growth factor (EGF) in human serum samples using an ultramicroanalytical system.
abstract: Human epidermal growth factor is a small peptide consisting of 53 amino acid residues, which stimulates cell proliferation and is associated with several human carcinomas. A simple sandwich-type ultramicroELISA assay (UMELISA), based on the advantages of high affinity reaction between streptavidin and biotin has been developed for the measurement of EGF in human serum samples. Strips coated with a high affinity monoclonal antibody directed against EGF are used as solid phase, to ensure the specificity of the assay. The EGF assay was completed in 18 hr, with a measuring range of 39-2500 pg/mL. The intra- and inter-assay coefficients of variation were 4.4-7.3% and 0-5.1%, respectively, depending on the EGF concentrations evaluated. Percentage recovery ranged from 96-104%. Regression analysis showed a good correlation with the commercially available Human EGF Immunoassay Quantikine®ELISA kit (n = 130, r = 0.92, P < 0.01). The analytical performance characteristics of our UMELISA EGF endorse its use for the quantification of EGF in human serum samples.
authors.affiliation: a Neonatal Screening Laboratory , Immunoassay Center , Havana , Cuba.
authors.forename: Elisa María
authors.initials: EM
authors.lastname: Castells Martínez
authors.affiliation: b Prenatal Screening Laboratory , Immunoassay Center , Havana , Cuba.
authors.forename: Ruben
authors.initials: R
authors.lastname: Del Valle
authors.affiliation: a Neonatal Screening Laboratory , Immunoassay Center , Havana , Cuba.
authors.forename: Ernesto Carlos
authors.initials: EC
authors.lastname: González
authors.affiliation: c Department of Protein Purification , Immunoassay Center , Havana , Cuba.
authors.forename: Antonio
authors.initials: A
authors.lastname: Melchor
authors.affiliation: a Neonatal Screening Laboratory , Immunoassay Center , Havana , Cuba.
authors.forename: Pedro Lucio
authors.initials: PL
authors.lastname: Pérez
authors.affiliation: d Department of Biomarkers , Center of Molecular Immunology , Havana , Cuba.
authors.forename: Idania
authors.initials: I
authors.lastname: González
authors.affiliation: d Department of Biomarkers , Center of Molecular Immunology , Havana , Cuba.
authors.forename: Adriana
authors.initials: A
authors.lastname: Carr
authors.affiliation: d Department of Biomarkers , Center of Molecular Immunology , Havana , Cuba.
authors.forename: Kalet
authors.initials: K
authors.lastname: León
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004815:Epidermal Growth Factor / Q000097:blood* / Q000276:immunology; D006801:Humans; D008832:Microchemistry / Q000379:methods*; D012044:Regression Analysis
chemical_list: D000911:Antibodies, Monoclonal; D004815:Epidermal Growth Factor
keywords: CIMAVax-EGF®; SUMA technology; epidermic growth factor; ultramicroELISA