title: MicroRNA-552 enhances metastatic capacity of colorectal cancer cells by targeting a disintegrin and metalloprotease 28.
abstract: Colorectal cancer (CRC) is one of the most common prevalent cancer types worldwide. MicroRNAs (miRNAs or miRs) have been demonstrated to play crucial roles in the development, metastasis and drug resistance of CRC. In the present study, a strikingly elevated expression of miR-552 was determined in CRC tumor tissues and cells by a miRNA profiling analysis. Importantly, the gene of A Disintegrin And Metalloprotease (ADAM) family member 28 (ADAM28) was identified as a target of miR-552, which was further validated in terms of genetic dual luciferase report assay. Furthermore, an inhibition of miR-552 in LOVE and LS174T CRC cells by transducing miR-552 inhibitor (antagomiR-552) with a lentiviral vector exhibited an ability to reduce cell proliferation, migration and clonogenicity. Moreover, both LOVO and LS174T cells stably expressing miR-552 inhibitor displayed a decreased ability to develop tumors in a murine xenograft model in vivo. In contrast, a knockdown of ADAM28 by short hairpin RNA could reverse the antagomiR-552-induced inhibition of metastatic features of CRC cells in vitro. These results suggested that miR-552 is an oncomir able to promote CRC metastasis in part through a mechanism of targeting ADAM28, which may be a novel target for CRC treatment and warrants for further investigation.
authors.affiliation: The general hospital, Ningxia Medical University, Yinchuan 750004, China.|Human Stem Cell Institute of the General Hospital at Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Colorectal Surgery, the General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Li
authors.affiliation: The general hospital, Ningxia Medical University, Yinchuan 750004, China.|Human Stem Cell Institute of the General Hospital at Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Human Stem Cell Institute of the General Hospital at Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Libin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Human Stem Cell Institute of the General Hospital at Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Xiurui
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Colorectal Surgery, the General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Colorectal Surgery, the General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Ma
authors.affiliation: The general hospital, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Du
authors.affiliation: Human Stem Cell Institute of the General Hospital at Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liu
authors.affiliation: The general hospital, Ningxia Medical University, Yinchuan 750004, China.|Human Stem Cell Institute of the General Hospital at Ningxia Medical University, Yinchuan 750004, China.|Department of Colorectal Surgery, the General Hospital of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Yinxue
authors.initials: Y
authors.lastname: Yang
mesh_terms: D051722:ADAM Proteins / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D009362:Neoplasm Metastasis
chemical_list: C000588992:MIRN552 microRNA, human; C000588991:MIRN592 microRNA, human; D035683:MicroRNAs; D051722:ADAM Proteins; C410224:ADAM28 protein, human
keywords: ADAM28; antagomir; colorectal cancer; miR-552; microRNAs