title: Experimental Model of Intervertebral Disk Mediated Postoperative Epidural Fibrosis.
abstract: BACKGROUND
  Postoperative epidural fibrosis (EF) after lumbar discectomy is the most common and at the same time controversial issue.
  
  
  OBJECTIVE
  The etiology and pathogenesis creates a lot of discussion and selection of methods of treatment and prevention continues.
  
  
  METHODS
  LIV laminectomy with dura mater (DM) exposition was done in 24 rats, and then, 0.3 ml of elements of suspension of autologous intervertebral disk was implicated on DM. As autologous intervertebral disk, we used the intervertebral disk from amputated tail. In all the animals, incisions were closed with 3/0 Vicryl. EF was examined. Fibroblast cell density was calculated in each field at ×40 magnification: Grade 1 - fewer than 100 fibroblasts in each field; Grade 2 - 100-150 fibroblasts in each field; Grade 3 - more than 150 fibroblasts in each field.
  
  
  RESULTS
  Based on histological results, we confirmed our model of experiment. On the 30th day of evaluation, there were significant histological evidences of postoperative epidural adhesions in experimental animals, which included the obliteration of epidural space, the presence of adhesions in the dura and nerve roots, the restructuring of the yellow ligament, bone sclerosis, excessive appearance of fibrous tissue around the autologous intervertebral disk tissue that applied on the DM.
  
  
  CONCLUSIONS
  In our work, we describe a new experimental model, where the elements of autologous intervertebral disk play the role of inflammation trigger, which cause postoperative scar and EF.
authors.affiliation: Scientific Center of Reconstructive and Restorative Surgery, RAMS, Siberian Branch, Irkutsk, Russia; Department of Traumatology, Orthopedics and Neurosurgery, Irkutsk State Medical Academy of Continuous Education, Irkutsk, Russia; Department of Child's Neurosurgery, A.L. Polenov Russian Neurosurgery Research Institute, Saint Petersburg, Russia.
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Larionov
authors.affiliation: Scientific Center of Reconstructive and Restorative Surgery, RAMS, Siberian Branch, Irkutsk, Russia; Department of Traumatology, Orthopedics and Neurosurgery, Irkutsk State Medical Academy of Continuous Education, Irkutsk, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Sorokovikov
authors.affiliation: Scientific Center of Reconstructive and Restorative Surgery, RAMS, Siberian Branch, Irkutsk, Russia.
authors.forename: K C
authors.initials: KC
authors.lastname: Erdyneyev
authors.affiliation: Scientific Center of Reconstructive and Restorative Surgery, RAMS, Siberian Branch, Irkutsk, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Lepekhova
authors.affiliation: Scientific Center of Reconstructive and Restorative Surgery, RAMS, Siberian Branch, Irkutsk, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Goldberg
keywords: Experimental model; Intervertebral disk; Laminectomy; Postoperative epidural fibrosis