title: Functional liver tissue engineering by an adult mouse liver-derived neuro-glia antigen 2-expressing stem/progenitor population.
abstract: Deaths due to end-stage liver diseases are increasingly registered annually in the world. Liver transplantation is the ultimate treatment for end-stage liver diseases to date, which has been hampered by a critical shortage of organs. The potential of decellularized liver scaffolds (DLS) derived from solid organs as a three-dimensional platform has been evolved as a promising approach in liver tissue engineering for translating functional liver organ replacements, but questions still exist regarding the optimal cell population for seeding in DLS and the preparation of the DLS themselves. The aim of our study was to utilize a sodium dodecyl sulfate decellularization procedure in combination with a low concentration of trypsin (0.005%)-ethylenediaminetetraacetic acid (0.002%) process to manufacture DLS from whole mouse livers and recellularized with hepatic stem/progenitors for use in liver tissue engineering and injured liver treatment. Results showed that the DLS generated with all the necessary microstructure and the extracellular components to support seeded hepatic stem/progenitor cell attachment, functional hepatic cell differentiation. Hepatic differentiation from stem/progenitor cells loaded by DLS was more efficient than that of the stem/progenitor cells in the two-dimensional cell culture model. In summary, the method of DLS loaded by hepatic stem/progenitor cells provided by this study was effective in maintaining DLS extracellular matrix to introduce seeded stem/progenitor cell differentiation, hepatic-like tissue formation and functional hepatic protein production in vitro that promoted functional recovery and survival in a mouse model of dimethylnitrosamine-induced liver cirrhosis after auxiliary heterotopic liver transplantation. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Hepatobiliary Institute, Southwestern Hospital, No 30. Gaotanyan, ShapingBa Distract, Chongqing, 400038, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Surgery, Division of Hepatobiliary and Abdominal Organ Transplantation, Case Western Reserve University Hospital, Cleveland, OH, 44106, USA.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Siegel
authors.affiliation: Hepatobiliary Institute, Southwestern Hospital, No 30. Gaotanyan, ShapingBa Distract, Chongqing, 400038, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Hepatobiliary Institute, Southwestern Hospital, No 30. Gaotanyan, ShapingBa Distract, Chongqing, 400038, China.
authors.forename: Jiejuan
authors.initials: J
authors.lastname: Lai
authors.affiliation: Hepatobiliary Institute, Southwestern Hospital, No 30. Gaotanyan, ShapingBa Distract, Chongqing, 400038, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Shuai
authors.affiliation: Hepatobiliary Institute, Southwestern Hospital, No 30. Gaotanyan, ShapingBa Distract, Chongqing, 400038, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Lai
authors.affiliation: Hepatobiliary Institute, Southwestern Hospital, No 30. Gaotanyan, ShapingBa Distract, Chongqing, 400038, China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Hepatobiliary Institute, Southwestern Hospital, No 30. Gaotanyan, ShapingBa Distract, Chongqing, 400038, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Hepatobiliary Institute, Southwestern Hospital, No 30. Gaotanyan, ShapingBa Distract, Chongqing, 400038, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Bie
authors.affiliation: Hepatobiliary Institute, Southwestern Hospital, No 30. Gaotanyan, ShapingBa Distract, Chongqing, 400038, China.
authors.forename: Lianhua
authors.initials: L
authors.lastname: Bai
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000941:Antigens / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004052:Diethylnitrosamine; D004492:Edetic Acid / Q000494:pharmacology; D008099:Liver / Q000502:physiology*; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000473:pathology; D016031:Liver Transplantation; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010477:Perfusion; D011509:Proteoglycans / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013234:Stem Cells / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014357:Trypsin / Q000494:pharmacology; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D004052:Diethylnitrosamine; D004492:Edetic Acid; D011509:Proteoglycans; D012967:Sodium Dodecyl Sulfate; D014357:Trypsin; D000099216:Chondroitin Sulfate Proteoglycan 4
keywords: cell differentiation; decellularized liver scaffold; hepatic stem/progenitors; liver cirrhosis; liver regeneration; recellularization; tissue engineering; tissue repair