title: Optimization of pretreatment, enzymatic hydrolysis and fermentation for more efficient ethanol production by Jerusalem artichoke stalk.
abstract: Jerusalem artichoke (JA) is a potential energy crop for biorefinery due to its unique agronomic traits such as resistance to environmental stresses and high biomass yield in marginal lands. Although JA tubers have been explored for inulin extraction and biofuels production, there is little concern on its stalk (JAS). In this article, the pretreatment of JAS by alkaline hydrogen peroxide was optimized using the response surface methodology to improve sugars yield and reduce chemicals usage. Scanning electron microscopy, X-ray diffraction, and thermogravimetric analysis were applied to characterize the structures of the pretreated JAS to evaluate the effectiveness of the pretreatment. Furthermore, the feeding of the pretreated JAS and cellulase was performed for high solid uploading (up to 30%) to increase ethanol titer, and simultaneous saccharification and fermentation with 55.6g/L ethanol produced, 36.5% more than that produced through separate hydrolysis and fermentation, was validated to be more efficient.
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: likai.sjtu@sjtu.edu.cn.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116023, China. Electronic address: qjch19920628@mail.dlut.edu.cn.
authors.forename: Jin-Cheng
authors.initials: JC
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: cg.liu@sjtu.edu.cn.
authors.forename: Chen-Guang
authors.initials: CG
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116023, China. Electronic address: fwbai@sjtu.edu.cn.
authors.forename: Feng-Wu
authors.initials: FW
authors.lastname: Bai
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D044602:Cellulases / Q000737:chemistry / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006368:Helianthus / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D006868:Hydrolysis; D008855:Microscopy, Electron, Scanning; D035281:Plant Tubers / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D056804:Biofuels; D000431:Ethanol; D006861:Hydrogen Peroxide; D044602:Cellulases
keywords: Enzymatic hydrolysis; Ethanol fermentation; Jerusalem artichoke stalk; Pretreatment; Response surface methodology