title: Electroinduced Delivery of Hydrogel Nanoparticles in Colon 26 Cells, Visualized by Confocal Fluorescence System.
abstract: Nano-scale drug delivery systems (nano-DDS) are under intense investigation. Nano-platforms are developed for specific administration of small molecules, drugs, genes, contrast agents [quantum dots (QDs)] both in vivo and in vitro. Electroporation is a biophysical phenomenon which consists of the application of external electrical pulses across the cell membrane. The aim of this study was to research electro-assisted Colon 26 cell line internalization of QDs and QD-loaded nano-hydrogels (polymersomes) visualized by confocal microscopy and their influence on cell viability.
  
  
  
  The experiments were performed on the Colon 26 cancer cell line, using a confocal fluorescent imaging system and cell viability test.
  
  
  
  Electroporation facilitated the delivery of nanoparticles in vivo. We demonstrated increased voltage-dependent delivery of nanoparticles into cells after electrotreatment, without significant cell viability reduction.
  
  
  
  The delivery and retention of the polymersomes in vitro is a promising tool for future cancer treatment strategies and nanomedcine.
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria.
authors.forename: Severina
authors.initials: S
authors.lastname: Atanasova
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria nikolova@bio21.bas.bg bakalova@nirs.go.jp.
authors.forename: Biliana
authors.initials: B
authors.lastname: Nikolova
authors.affiliation: Department of Molecular Imaging and Theranostics, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology, Chiba, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Murayama
authors.affiliation: Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, Sofia, Bulgaria.
authors.forename: Elena
authors.initials: E
authors.lastname: Stoyanova
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria.
authors.forename: Iana
authors.initials: I
authors.lastname: Tsoneva
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria Department of Physics, Biophysics and Radiology, Medical Faculty, Sofia University, Sofia, Bulgaria Department of Medical Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora, Bulgaria.
authors.forename: Zhivko
authors.initials: Z
authors.lastname: Zhelev
authors.affiliation: Department of Molecular Imaging and Theranostics, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology, Chiba, Japan.
authors.forename: Ichio
authors.initials: I
authors.lastname: Aoki
authors.affiliation: Department of Molecular Imaging and Theranostics, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology, Chiba, Japan Department of Physics, Biophysics and Radiology, Medical Faculty, Sofia University, Sofia, Bulgaria nikolova@bio21.bas.bg bakalova@nirs.go.jp.
authors.forename: Rumiana
authors.initials: R
authors.lastname: Bakalova
mesh_terms: D000818:Animals; D001703:Biophysics; D045744:Cell Line, Tumor / Q000187:drug effects; D002470:Cell Survival; D003110:Colonic Neoplasms / Q000378:metabolism*; D003287:Contrast Media / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D018274:Electroporation; D005260:Female; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D009368:Neoplasm Transplantation; D011108:Polymers / Q000737:chemistry; D045663:Quantum Dots
chemical_list: D003287:Contrast Media; D020100:Hydrogels; D011108:Polymers
keywords: Nano-scale drug delivery systems; QD; colon cancer cells; electroporation; nano-DDS; quantum dots