title: Label-free detection of fibrillar collagen deposition associated with vascular elements in glioblastoma multiforme by using multiphoton microscopy.
abstract: Glioblastoma multiforme (GBM-WHO grade IV) is the most common and the most aggressive form of brain tumors in adults with the median survival of 10-12 months. The diagnostic detection of extracellular matrix (ECM) component in the tumour microenvironment is of prognostic value. In this paper, the fibrillar collagen deposition associated with vascular elements in GBM were investigated in the fresh specimens and unstained histological slices by using multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG). Our study revealed the existence of fibrillar collagen deposition in the adventitia of remodelled large blood vessels and in glomeruloid vascular structures in GBM. The degree of fibrillar collagen deposition can be quantitatively evaluated by measuring the adventitial thickness of blood vessels or calculating the ratio of SHG pixel to the whole pixel of glomeruloid vascular structure in MPM images. These results indicated that MPM can not only be employed to perform a retrospective study in unstained histological slices but also has the potential to apply for in vivo brain imaging to understand correlations between malignancy of gliomas and fibrillar collagen deposition.
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China.|Department of Physics, Chung Yuan Christian University, Chung-Li, Taiwan.
authors.forename: L W
authors.initials: LW
authors.lastname: Jiang
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wu
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China.
authors.forename: P H
authors.initials: PH
authors.lastname: Lin
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China.
authors.forename: H P
authors.initials: HP
authors.lastname: Du
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China.
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China.
authors.forename: L H
authors.initials: LH
authors.lastname: Li
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China.
authors.forename: N
authors.initials: N
authors.lastname: Fang
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China.
authors.forename: S M
authors.initials: SM
authors.lastname: Zhuo
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
authors.forename: D Z
authors.initials: DZ
authors.lastname: Kang
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Chen
mesh_terms: D001808:Blood Vessels / Q000473:pathology*; D001932:Brain Neoplasms / Q000175:diagnosis / Q000473:pathology*; D024022:Fibrillar Collagens / Q000032:analysis*; D005909:Glioblastoma / Q000175:diagnosis / Q000473:pathology*; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D011379:Prognosis; D012189:Retrospective Studies
chemical_list: D024022:Fibrillar Collagens
keywords: Fibrillar collagen deposition; glioblastoma multiforme; multiphoton microscopy; second harmonic generation; two-photon excited fluorescence