title: Dehydroabietic acid cytotoxicity in goldfish radial glial cells in vitro.
abstract: Dehydroabietic acid (DHAA) is a resin acid present in aquatic environments shown to induce cellular and molecular damage in aquatic animals. In this study, the cytotoxicity of DHAA on primary cultured goldfish radial glial cells (RGCs), an important component of the central nervous system, was evaluated. Here, it is reported that a concentration of 20mg/L DHAA affected cellular morphology and expression of genes involved in RGC steroidogenesis and metabolism. Higher concentration exposures of DHAA (40mg/L) lead to RGC death based on a lactate dehydrogenase leakage assay. Together, these data have implications in understanding the effects of DHAA on an integral central nervous system cell type important for neurogenesis, steroidogenesis and structural support. Due to the continuous presence of DHAA into water systems, results from this study provide indications as to the potential impacts of DHAA and demonstrate the importance of this class of chemicals on aquatic organisms.
authors.affiliation: Department of Biology, University of Ottawa, Ottawa, Ontario, K1N 6N5, Canada.
authors.forename: Lei
authors.initials: L
authors.lastname: Xing
authors.affiliation: Department of Biology, University of Ottawa, Ottawa, Ontario, K1N 6N5, Canada.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Gutierrez-Villagomez
authors.affiliation: Department of Biology, University of Ottawa, Ottawa, Ontario, K1N 6N5, Canada.
authors.forename: Dillon F
authors.initials: DF
authors.lastname: Da Fonte
authors.affiliation: Department of Biology, University of Ottawa, Ottawa, Ontario, K1N 6N5, Canada.
authors.forename: Maddie J
authors.initials: MJ
authors.lastname: Venables
authors.affiliation: Department of Biology, University of Ottawa, Ottawa, Ontario, K1N 6N5, Canada. Electronic address: trudeauv@uottawa.ca.
authors.forename: Vance L
authors.initials: VL
authors.lastname: Trudeau
mesh_terms: D045784:Abietanes / Q000633:toxicity*; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D063928:Ependymoglial Cells / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D005260:Female; D006054:Goldfish; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D045784:Abietanes; D014874:Water Pollutants, Chemical; C013913:dehydroabietic acid
keywords: Cell morphology; DHAA; Radial glial cell; Steroidogenesis