title: MicroRNA Signatures of Colonic Polyps on Screening and Histology.
abstract: Colorectal cancer and adenoma adjacent to cancer exhibit distinct microRNA (miRNA) alterations in an apparent mucosa-to-adenocarcinoma sequence. The pattern of microRNAs in screen-detected polyps in relation to histologic features and cancer risk has not been investigated. miRNA expression analysis was performed on normal mucosa (NM), hyperplastic polyps (HP), tubular adenomas (TA), tubulovillous adenomas or high-grade dysplasia (TVHG), and serrated polyps [sessile serrated adenoma/polyps (SSA/P) and traditional serrated adenomas (TSA)] in biopsy specimens from 109 patients undergoing screening/surveillance colonoscopy. Generalized linear models were used to identify differentially expressed miRNAs by histologic type and logistic regression to identify miRNA predictors of histopathology. False discovery rate (FDR) was used to control for multiple comparisons. We identified 99 miRNAs differing in at least one of five histopathologic groups (FDR ≤0.05). In a comparison of HPNM versus TVHG, the top most upregulated and downregulated miRNAs in HPNM included miR-145, -143, -107, -194, and -26a (upregulated), and miR-663, -1268, -320b, -1275, and -320b (downregulated; FDR P < 0.05). miR-145 and -619 showed high accuracy to discriminate low- from high-risk polyps without serrated histology (TVHG vs. HPNM + TA; CI, 95.6%), whereas miR-124, -143, and -30a showed high accuracy of separating high-risk polyps (TVHG + TSA) from low-risk polyps (HPNM + TA + SSA/P; CI, 96.0%). For TSAs, miR-125b and -199a were uniquely downregulated relative to HPNMs, and miR-335, -222, and -214 discriminated between non-serrated and serrated histology. Our data support the presence of colorectal cancer-associated miRNA alterations in screen-detected adenomas that may be useful for risk stratification for surveillance interval planning. Cancer Prev Res; 9(12); 942-9. ©2016 AACR.
authors.affiliation: Department of Surgery, Oregon Health and Science University, Portland, Oregon. tsikitis@ohsu.edu.
authors.forename: Vassiliki L
authors.initials: VL
authors.lastname: Tsikitis
authors.affiliation: Oregon Health and Science University, Integrated Genomics Laboratory, Portland, Oregon.
authors.forename: Amiee
authors.initials: A
authors.lastname: Potter
authors.affiliation: Oregon Health and Science University, Integrated Genomics Laboratory, Portland, Oregon.|Oregon Health and Science University, Knight Cancer Institute, Portland, Oregon.
authors.forename: Motomi
authors.initials: M
authors.lastname: Mori
authors.affiliation: Stony Brook University School of Medicine, Stony Brook, New York.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Buckmeier
authors.affiliation: Stony Brook University School of Medicine, Stony Brook, New York.
authors.forename: Christina R
authors.initials: CR
authors.lastname: Preece
authors.affiliation: Oregon Health and Science University, Integrated Genomics Laboratory, Portland, Oregon.
authors.forename: Christina A
authors.initials: CA
authors.lastname: Harrington
authors.affiliation: Integrated Healthcare Associates, Dept of Anatomic and Clinical Pathology, Ann Arbor, Michigan.
authors.forename: Angela N
authors.initials: AN
authors.lastname: Bartley
authors.affiliation: Department of Pathology, University of Arizona Cancer Center, Tucson, Arizona.
authors.forename: Achyut K
authors.initials: AK
authors.lastname: Bhattacharyya
authors.affiliation: Integrated Healthcare Associates, Dept of Anatomic and Clinical Pathology, Ann Arbor, Michigan.
authors.forename: Stanley R
authors.initials: SR
authors.lastname: Hamilton
authors.affiliation: Department of Molecular and Cell Biology, University of Arizona Cancer Center, Tucson, Arizona.
authors.forename: M Peter
authors.initials: MP
authors.lastname: Lance
authors.affiliation: Stony Brook University School of Medicine, Stony Brook, New York.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Thompson
mesh_terms: D000236:Adenoma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D000368:Aged; D003111:Colonic Polyps / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D003113:Colonoscopy; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D055088:Early Detection of Cancer / Q000379:methods; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D016015:Logistic Models; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D012306:Risk
chemical_list: D035683:MicroRNAs