title: Analysis of Senescence-Related Differentiation Potentials and Gene Expression Profiles in Human Dental Pulp Stem Cells.
abstract: BACKGROUND
  Dental pulp stem cell (DPSC)-mediated dental pulp regeneration is considered a promising method for the treatment of deep caries with pulpitis. However, mesenchymal stem cell (MSC) senescence is an adverse factor from the perspective of cell-based therapies. In this study, we investigated the characteristics and expression profiles of DPSCs from young and old donors.
  
  
  METHODS
  DPSCs from young and old donors were cultured in differentiation medium, and their differentiation potentials were assessed. Long noncoding RNA (LncRNA) microarray assays and a bioinformatic analysis were performed to investigate differences in LncRNA and mRNA expression profiles between DPSCs from young and old donors.
  
  
  RESULTS
  We found that DPSCs from young donors exhibited more powerful proliferation ability and greater osteogenic and adipogenic differentiation potentials than DPSCs from old donors. In DPSCs from young donors, numerous LncRNAs were significantly up- (n = 389) or down-regulated (n = 172) compared to DPSCs from old donors. Furthermore, 304 mRNAs were differentially expressed, including 247 up-regulated genes and 57 down-regulated genes in DPSCs from young donors. The bioinformatic analysis identified that several pathways may be associated with DPSC characteristics, such as those involved in the cell cycle and RNA transport, and revealed nuclear transcription factor Y subunit β, general transcription factor IIB, and nuclear receptor subfamily 3 group C member 1 as core regulatory factors and FR249114, FR299091, and ENST00000450004 as core LncRNAs.
  
  
  CONCLUSIONS
  Our results indicated that senescence impaired the proliferation and differentiation potentials of DPSCs and that donor age is an important factor that affects their use for tooth regeneration. We also provide insight into the mechanisms responsible for senescence in DPSCs.
authors.affiliation: Xiangya Stomatology Hospital, Changsha, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Yi
authors.forename: Ousheng
authors.initials: O
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.forename: Xiao
authors.initials: X
authors.lastname: Lin
authors.forename: Shu
authors.initials: S
authors.lastname: Diao
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.forename: Luyuan
authors.initials: L
authors.lastname: Jin
authors.forename: Songlin
authors.initials: S
authors.lastname: Wang
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Lu
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Fan
mesh_terms: D050156:Adipogenesis; D000293:Adolescent; D000328:Adult; D000368:Aged; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation; D002469:Cell Separation; D016922:Cellular Senescence / Q000235:genetics*; D002648:Child; D019295:Computational Biology; D003782:Dental Pulp / Q000166:cytology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010012:Osteogenesis; D062085:RNA, Long Noncoding / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D014019:Tissue Donors; D055815:Young Adult
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger