title: Experimental study on co-culturing adipose-derived stem cells with osteoblasts under different conditions.
abstract: To observe whether adipose-derived stem cells (ADSCS), co-cultured with osteoblasts, can differentiate into osteoblasts and, if so, to study the best-induced conditions, with an ultimate goal of repairing bone defects.
  
  
  
  Adipose-derived stem cells and osteoblasts were isolated from New Zealand white rabbits, and co-cultured in media with either 5% or 10% fetal bovine serum, for up to 4 weeks. The morphology of collected cells was examined under a microscope, and histological staining with alkaline phosphatase and alizarin red was carried out after induction for 1, 2, 3 and 4 weeks. Osteogenesis identification, including mRNA expression of type I collagen and osteocalcin, and alkaline phosphatase, was also performed using RT-PCR.
  
  
  
  After 7 days of co-culture, some adipose-derived stem cells became round in both groups. After 14 days of co-culture, adipose-derived stem cells were found highly-differentiated, and stained positively with alkaline phosphatase and alizarin red, similar to mature osteoblasts. The mRNA expression of type I collagen and osteocalcin increased in both groups, especially in the 10% fetal bovine serum group.
  
  
  
  Our findings indicate that adipose-derived stem cells co-cultured with osteoblasts can differentiate into osteoblasts when induced by a high concentration of serum culture.
authors.affiliation: Department of Orthopaedics, the First Hospital of Xuzhou, Xuzhou, China. yangg2doc@163.com.
authors.forename: D-C
authors.initials: DC
authors.lastname: Liu
authors.forename: X-N
authors.initials: XN
authors.lastname: Yang
authors.forename: C-Z
authors.initials: CZ
authors.lastname: Huang
authors.forename: Y-G
authors.initials: YG
authors.lastname: Sun
authors.forename: X-M
authors.initials: XM
authors.lastname: Dai
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D010006:Osteoblasts; D010012:Osteogenesis; D011817:Rabbits; D013234:Stem Cells