title: Gastrodin Pretreatment Impact on Sarcoplasmic Reticulum Calcium Transport ATPase (SERCA) and Calcium Phosphate (PLB) Expression in Rats with Myocardial Ischemia Reperfusion.
abstract: BACKGROUND Calcium overload, inflammation, and apoptosis play important roles in myocardial ischemia-reperfusion injury (MIRI). Gastrodin pretreatment can alleviate MIRI. This study observed sarcoplasmic reticulum calcium transport ATPase (Ca2+-ATPase, SERCA) and calcium phosphate (PLB) protein expression in the ventricular remodeling process after myocardial infarction to explore the effect of gastrodin pretreatment on MIRI. MATERIAL AND METHODS Healthy 7-week-old male SD rats were randomly divided into a sham group (A), a model group (B), and gastrodin pretreatment groups C, D, and E (100, 200, and 400 mg/kg, respectively) with 20 in each group. Anterior descending coronary artery ligation method was used to establish a rat MIRI model with 30-min ischemia and 120-min reperfusion. Cardiac electrophysiological activity was recorded. Serum IL-6 and IL10 levels were determined by ELISA. SERCA activity was tested by colorimetric phosphorus method. SERCA, PLB, and pSer-PLB protein expression were detected by Western blot. RESULTS Compared with the sham group, IL-6 and IL-10 levels were elevated, SERCA2a expression was downregulated, and PLB protein was elevated in the model group (P<0.05). pSer16-PLB showed no significant difference among groups, and the ratio of pSer16-PLB/PLB obviously decreased (P<0.05). IL-6 level gradually declined and IL-10 increased in the gastrodin group following concentration elevation. SERCA 2a expression rose in the gastrodin group in a dose-dependent manner (P<0.05). Elevated PLB protein expression showed no significant difference, while pSer16-PLB protein increased (P<0.05), leading to elevated pSer16 PLB/PLB ratio (P<0.05). CONCLUSIONS Gastrodin pretreatment alleviates MIRI and inflammation injury by regulating SERCA and PLB expression to decrease calcium overload.
authors.affiliation: Department of ENT, Linyi City People's Hospital, Linyi, Shandong, China (mainland).
authors.forename: Yufen
authors.initials: Y
authors.lastname: Li
authors.affiliation: Genetics Laboratory, Women and Children's Hospital of Linyi City, Linyi, Shandong, China (mainland).
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of ENT, Linyi City People's Hospital, Linyi, Shandong, China (mainland).
authors.forename: Changli
authors.initials: C
authors.lastname: Lou
mesh_terms: D000818:Animals; D001592:Benzyl Alcohols / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002130:Calcium Phosphates / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D003324:Coronary Artery Disease / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism / Q000628:therapy*; D015425:Myocardial Reperfusion / Q000379:methods*; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000096:biosynthesis*
chemical_list: D001592:Benzyl Alcohols; D002130:Calcium Phosphates; D002135:Calcium-Binding Proteins; D005960:Glucosides; C045345:gastrodin; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium