title: Characterization and Transposon Mutagenesis of the Maize (Zea mays) Pho1 Gene Family.
abstract: Phosphorus is an essential nutrient for all plants, but also one of the least mobile, and consequently least available, in the soil. Plants have evolved a series of molecular, metabolic and developmental adaptations to increase the acquisition of phosphorus and to maximize the efficiency of use within the plant. In Arabidopsis (Arabidopsis thaliana), the AtPHO1 protein regulates and facilitates the distribution of phosphorus. To investigate the role of PHO1 proteins in maize (Zea mays), the B73 reference genome was searched for homologous sequences, and four genes identified that were designated ZmPho1;1, ZmPho1;2a, ZmPho1;2b and ZmPho1;3. ZmPho1;2a and ZmPho1;2b are the most similar to AtPHO1, and represent candidate co-orthologs that we hypothesize to have been retained following whole genome duplication. Evidence was obtained for the production of natural anti-sense transcripts associated with both ZmPho1;2a and ZmPho1;2b, suggesting the possibility of regulatory crosstalk between paralogs. To characterize functional divergence between ZmPho1;2a and ZmPho1;2b, a program of transposon mutagenesis was initiated using the Ac/Ds system, and, here, we report the generation of novel alleles of ZmPho1;2a and ZmPho1;2b.
authors.affiliation: Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Unidad de Genómica Avanzada, Centro de Investigaciones y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato C.P. 36821, Guanajuato, México.
authors.forename: M Nancy
authors.initials: MN
authors.lastname: Salazar-Vidal
authors.affiliation: Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Unidad de Genómica Avanzada, Centro de Investigaciones y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato C.P. 36821, Guanajuato, México.
authors.forename: Edith
authors.initials: E
authors.lastname: Acosta-Segovia
authors.affiliation: Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Unidad de Genómica Avanzada, Centro de Investigaciones y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato C.P. 36821, Guanajuato, México.
authors.forename: Nidia
authors.initials: N
authors.lastname: Sánchez-León
authors.affiliation: Boyce Thompson Institute for Plant Research, Ithaca, New York 14853-1801, United States of America.
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Ahern
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, Missouri 63132, United States of America.
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Brutnell
authors.affiliation: Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Unidad de Genómica Avanzada, Centro de Investigaciones y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato C.P. 36821, Guanajuato, México.
authors.forename: Ruairidh J H
authors.initials: RJ
authors.lastname: Sawers
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D005810:Multigene Family; D016296:Mutagenesis; D010710:Phosphates / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D004251:DNA Transposable Elements; C460111:PHO1 protein, Arabidopsis; D010710:Phosphates; D010940:Plant Proteins