title: Benefits of Bifidobacterium animalis subsp. lactis Probiotic in Experimental Periodontitis.
abstract: This study evaluates effects of topical administration of probiotic bacteria of the genus Bifidobacterium on experimental periodontitis (EP) in rats.
  
  
  
  Thirty-two rats were divided into groups C (control; without EP), EP (EP only), C-HN019 (control+probiotic), and EP-HN019 (EP+probiotic). On day 0 of the experiment, animals of groups EP and EP-HN019 received cotton ligatures around mandibular first molars (MFMs). In groups C-HN019 and EP-HN019, 1 mL of suspensions containing Bifidobacterium animalis subsp. lactis (B. lactis) HN019 was topically administered in the subgingival region of MFMs on days 0, 3, and 7. In groups C and EP, topical administrations were performed using a sham suspension (without probiotic). All animals were euthanized at day 14. Gingival tissue, hemimandibles, and oral biofilm were collected. Data were statistically analyzed (P <0.05).
  
  
  
  Group EP presented greater bone porosity, trabecular separation, and connective tissue attachment loss (CTAL) as well as reduced bone volume than all other groups (P <0.05). In group EP-HN019, there were greater proportions of Actinomyces and Streptococcus-like species and lower proportions of Veillonella parvula, Capnocytophaga sputigena, Eikenella corrodens, and Prevotella intermedia-like species than group EP. Group EP-HN019 presented greater expressions of osteoprotegerin and β-defensins than group EP (P <0.05). Group EP presented greater levels of interleukin-1β and receptor activator of nuclear factor-kappa B ligand than group EP-HN019 (P <0.05).
  
  
  
  Topical use of B. lactis HN019 promotes a protective effect against alveolar bone loss and CTALs attributable to EP in rats, modifying immunoinflammatory and microbiologic parameters.
authors.affiliation: Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of São Paulo, São Paulo, Brazil.
authors.forename: Luiz F F
authors.initials: LF
authors.lastname: Oliveira
authors.affiliation: Department of Clinical Analyses, School of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo.
authors.forename: Sérgio L
authors.initials: SL
authors.lastname: Salvador
authors.affiliation: Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of São Paulo, São Paulo, Brazil.
authors.forename: Pedro H F
authors.initials: PH
authors.lastname: Silva
authors.affiliation: Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of São Paulo, São Paulo, Brazil.
authors.forename: Flávia A C
authors.initials: FA
authors.lastname: Furlaneto
authors.affiliation: Department of Periodontology, Dental Research Division, Guarulhos University, São Paulo, Brazil.
authors.forename: Luciene
authors.initials: L
authors.lastname: Figueiredo
authors.affiliation: Department of Prosthodontics and Periodontics, School of Dentistry, Campinas State University, São Paulo, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Casarin
authors.affiliation: Department of Basic Sciences, Division of Histology, Dental School of Aracatuba, São Paulo State University, São Paulo, Brazil.
authors.forename: Edilson
authors.initials: E
authors.lastname: Ervolino
authors.affiliation: Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of São Paulo, São Paulo, Brazil.
authors.forename: Daniela B
authors.initials: DB
authors.lastname: Palioto
authors.affiliation: Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of São Paulo, São Paulo, Brazil.
authors.forename: Sérgio L S
authors.initials: SL
authors.lastname: Souza
authors.affiliation: Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of São Paulo, São Paulo, Brazil.
authors.forename: Mario
authors.initials: M
authors.lastname: Taba
authors.affiliation: Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of São Paulo, São Paulo, Brazil.
authors.forename: Arthur B
authors.initials: AB
authors.lastname: Novaes
authors.affiliation: Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of São Paulo, São Paulo, Brazil.
authors.forename: Michel R
authors.initials: MR
authors.lastname: Messora
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000069982:Bifidobacterium animalis / Q000502:physiology*; D018441:Biofilms; D015415:Biomarkers / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D007124:Immunoenzyme Techniques; D008297:Male; D010518:Periodontitis / Q000382:microbiology / Q000628:therapy*; D016133:Polymerase Chain Reaction; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D055114:X-Ray Microtomography
chemical_list: D015415:Biomarkers; D016207:Cytokines
keywords: Alveolar bone loss; Bifidobacterium; periodontal attachment loss; periodontitis; probiotics; rats