title: Chromatographic selectivity of poly(alkyl methacrylate-co-divinylbenzene) monolithic columns for polar aromatic compounds by pressure-driven capillary liquid chromatography.
abstract: In this study, divinylbenzene (DVB) was used as the cross-linker to prepare alkyl methacrylate (AlMA) monoliths for incorporating π-π interactions between the aromatic analytes and AlMA-DVB monolithic stationary phases in capillary LC analysis. Various AlMA/DVB ratios were investigated to prepare a series of 30% AlMA-DVB monolithic stationary phases in fused-silica capillaries (250-μm i.d.). The physical properties (such as porosity, permeability, and column efficiency) of the synthesized AlMA-DVB monolithic columns were investigated for characterization. Isocratic elution of phenol derivatives was first employed to evaluate the suitability of the prepared AlMA-DVB columns for small molecule separation. The run-to-run (0.16-1.20%, RSD; n = 3) and column-to-column (0.26-2.95%, RSD; n = 3) repeatabilities on retention times were also examined using the selected AlMA-DVB monolithic columns. The π-π interactions between the aromatic ring and the DVB-based stationary phase offered better recognition on polar analytes with aromatic moieties, which resulted in better separation resolution of aromatic analytes on the AlMA-DVB monolithic columns. In order to demonstrate the capability of potential environmental and/or food safety applications, eight phenylurea herbicides with single benzene ring and seven sulfonamide antibiotics with polyaromatic moieties were analyzed using the selected AlMA-DVB monolithic columns.
authors.affiliation: Department of Chemistry, Soochow University, 70 Linhsi Road, Shihlin, 11102, Taipei, Taiwan.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Lin
authors.affiliation: Department of Chemistry, Soochow University, 70 Linhsi Road, Shihlin, 11102, Taipei, Taiwan.
authors.forename: Chih-Chieh
authors.initials: CC
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Soochow University, 70 Linhsi Road, Shihlin, 11102, Taipei, Taiwan. Electronic address: msfuh@scu.edu.tw.
authors.forename: Ming-Ren
authors.initials: MR
authors.lastname: Fuh
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D008689:Methacrylates / Q000737:chemistry*; D010539:Permeability; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D011312:Pressure; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D011108:Polymers; D014753:Vinyl Compounds; C004985:divinyl benzene
keywords: Alkyl methacrylate (AlMA); Capillary liquid chromatography; Divinylbenzene (DVB); Polymer monolithic stationary phases