title: Effect of ceramide-1-phosphate transfer protein on intestinal bacterial translocation in severe acute pancreatitis.
abstract: OBJECTIVE
  The aim of the study was to investigate the effects of ceramide-1-phosphate transfer protein (CPTP) on the intestinal epithelial tight junction proteins in patients with severe acute pancreatitis (SAP).
  
  
  METHODS
  Fifty patients with SAP were classified into two groups according to the presence of bacterial translocation (BT) in the blood. Thirty healthy individuals were included in the control group. The presence of BT was analyzed by polymerase chain reaction. The expression of tight junction proteins and CPTP was determined using immunohistochemistry and western blotting.
  
  
  RESULTS
  Bacterial DNA was detected in the peripheral blood of 62.0% of the patients with SAP. The expression of CPTP and tight junction proteins in SAP patients was lower than that in healthy controls. Among the patients with SAP, those positive for BT(+) showed a lower level of CPTP and occluding (OC) and zonula occludens-1 (ZO-1) expression and a higher level of IVA cPLA2 expression than BT(-) patients. Moreover, the expression of CPTP was significantly associated with ZO-1 and showed a negative correlation with expression of IVA cPLA2 in SAP-BT(+) patients.
  
  
  CONCLUSIONS
  CPTP affects the expression of tight junction proteins and may protects the intestinal epithelial barrier by downregulating the expression of IVA cPLA2.
authors.affiliation: Qingdao University, Qingdao Municipal Hospital, Center of Colon and Rectum, n(o) 1 Jiaozhou Road, Qingdao 266011, Shandong Province, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Qingdao University, Qingdao Municipal Hospital, Center of Colon and Rectum, n(o) 1 Jiaozhou Road, Qingdao 266011, Shandong Province, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Li
authors.affiliation: Qingdao University, Qingdao Municipal Hospital, Center of Colon and Rectum, n(o) 1 Jiaozhou Road, Qingdao 266011, Shandong Province, China.
authors.forename: Yingjian
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Qingdao University, Qingdao Municipal Hospital, Center of Colon and Rectum, n(o) 1 Jiaozhou Road, Qingdao 266011, Shandong Province, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Qingdao University, Qingdao Municipal Hospital, Center of Colon and Rectum, n(o) 1 Jiaozhou Road, Qingdao 266011, Shandong Province, China.
authors.forename: Dehui
authors.initials: D
authors.lastname: Li
authors.affiliation: Qingdao University, Qingdao Municipal Hospital, Center of Colon and Rectum, n(o) 1 Jiaozhou Road, Qingdao 266011, Shandong Province, China.
authors.forename: Yibo
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Pingxiang People's Hospital, Center of Anorectal Branch, Pingxiang 337000, Jiangxi Province, China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Deng
authors.affiliation: Qingdao University, Qingdao Municipal Hospital, Center of Colon and Rectum, n(o) 1 Jiaozhou Road, Qingdao 266011, Shandong Province, China. Electronic address: phdzdl@yahoo.com.
authors.forename: Dianliang
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D018988:Bacterial Translocation; D002352:Carrier Proteins / Q000097:blood / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D019283:Pancreatitis, Acute Necrotizing / Q000097:blood / Q000378:metabolism* / Q000382:microbiology; D049294:Phospholipid Transfer Proteins; D012720:Severity of Illness Index
chemical_list: C583333:CPTP protein, human; D002352:Carrier Proteins; D049294:Phospholipid Transfer Proteins