title: Role of ligninolytic enzymes of white rot fungi (Pleurotus spp.) grown with azo dyes.
abstract: BACKGROUND
  Total three Pleurotus species (P. ostreatus, P. sapidus, P. florida) was compared for ligninolytic enzyme production grown with Coralene Golden Yellow, Coralene Navy Blue and Coralene Dark Red azo dyes in liquid medium under shaking condition.
  
  
  RESULTS
  The biodegradation competency varied from species to species and it was found that P. ostreatus, P. sapidus and P. florida to 20 ppm dye concentration shows 88, 92 and 98 % decolorization, respectively for all three dyes. Production pattern of laccase, manganese dependent peroxidase and lignin peroxidase were studied during the growth of the organisms for 10 days. Laccase was found to be the major extracellular ligninolytic enzyme produced by fungus with negligible detection of lignin peroxidases. In all concentration of three dye studied, maximum laccase activity was observed on day 8, for 20 mg/l of dye laccase specific activity was 1-1.58 U/mg in P. ostreatus, 0.5-0.78 U/mg in P. sapidus and 1-1.92 U/mg in P. florida. Different factors (dye concentration, pH, protein and sugar estimation) influencing the ability of Pleurotus species to degrade dyes is documented and degradation was attributed to microbial action irrespective of pH change. HPTLC analysis of samples indicated degradation of dyes into intermediate products.
  
  
  CONCLUSIONS
  Level of ligninolytic enzymes is playing a major role in degradation of dye, which is dependent on time of incubation and species of fungi.
authors.affiliation: Department of Biochemistry, Faculty of Science, The. M. S. University of Baroda, Vadodara, 390001 Gujarat India.
authors.forename: Prashant D
authors.initials: PD
authors.lastname: Kunjadia
authors.affiliation: Max Planck Institute of Developmental Biology, Tubingen, Germany.
authors.forename: Gaurav V
authors.initials: GV
authors.lastname: Sanghvi
authors.affiliation: Ashok & Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New Vallabh Vidyangar, 388021 India.
authors.forename: Anju P
authors.initials: AP
authors.lastname: Kunjadia
authors.affiliation: Department of Biotechnology, GeneOmbio Technologies, Krishna Chambers, Pune, India.
authors.forename: Pratap N
authors.initials: PN
authors.lastname: Mukhopadhyay
authors.affiliation: Department of Biochemistry, Saurashtra University, Rajkot, 360005 India.
authors.forename: Gaurav S
authors.initials: GS
authors.lastname: Dave
keywords: Ligninolytic enzymes; Pleurotus species; Submerged fermentation; Thin layer chromatography