title: Comprehensive genomic characterization of five canine lymphoid tumor cell lines.
abstract: BACKGROUND
  Leukemia/lymphoma cell lines have been critical in the investigation of the pathogenesis and therapy of hematological malignancies. While human LL cell lines have generally been found to recapitulate the primary tumors from which they were derived, appropriate characterization including cytogenetic and transcriptional assessment is crucial for assessing their clinical predictive value.
  
  
  RESULTS
  In the following study, five canine LL cell lines, CLBL-1, Ema, TL-1 (Nody-1), UL-1, and 3132, were characterized using extensive immunophenotyping, karyotypic analysis, oligonucleotide array comparative genomic hybridization (oaCGH), and gene expression profiling. Genome-wide DNA copy number data from the cell lines were also directly compared with 299 primary canine round cell tumors to determine whether the cell lines represent primary tumors, and, if so, what subtype each most closely resembled.
  
  
  CONCLUSIONS
  Based on integrated analyses, CLBL-1 was classified as B-cell lymphoma, Ema and TL-1 as T-cell lymphoma, and UL-1 as T-cell acute lymphoblastic leukemia. 3132, originally classified as a B-cell lymphoma, was reclassified as a histiocytic sarcoma based on characteristic cytogenomic properties. In combination, these data begin to elucidate the clinical predictive value of these cell lines which will enhance the appropriate selection of in vitro models for future studies of canine hematological malignancies.
authors.affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, CVM Research Building - Room 348, 1060 William Moore Drive, Raleigh, 27607, NC, USA.
authors.forename: Sarah C
authors.initials: SC
authors.lastname: Roode
authors.affiliation: Bioinformatics Research Center, Department of Statistics, North Carolina State University, Raleigh, NC, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Rotroff
authors.affiliation: Department of Medicine, University of North Carolina, Chapel Hill, NC, USA.|Comparative Medicine Institute, North Carolina State University, Raleigh, NC, USA.|Cancer Genetics Program, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA.|KLR current address: Department of Biomedical Sciences, Cornell University, Ithaca, NY, USA.
authors.forename: Kristy L
authors.initials: KL
authors.lastname: Richards
authors.affiliation: Department of Pathology, Microbiology, and Immunology, College of Veterinary Medicine, University of California, Davis, CA, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Moore
authors.affiliation: Bioinformatics Research Center, Department of Statistics, North Carolina State University, Raleigh, NC, USA.|Comparative Medicine Institute, North Carolina State University, Raleigh, NC, USA.
authors.forename: Alison
authors.initials: A
authors.lastname: Motsinger-Reif
authors.affiliation: Veterinary Teaching Hospital, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Okamura
authors.affiliation: Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Mizuno
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
authors.affiliation: Comparative Medicine Institute, North Carolina State University, Raleigh, NC, USA. steven_suter@ncsu.edu.|Cancer Genetics Program, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA. steven_suter@ncsu.edu.|Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, CVM Research Building - Room 308, 1051 William Moore Drive, Raleigh, NC, 27607, USA. steven_suter@ncsu.edu.
authors.forename: Steven E
authors.initials: SE
authors.lastname: Suter
authors.affiliation: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, CVM Research Building - Room 348, 1060 William Moore Drive, Raleigh, 27607, NC, USA. Matthew_Breen@ncsu.edu.|Comparative Medicine Institute, North Carolina State University, Raleigh, NC, USA. Matthew_Breen@ncsu.edu.|Cancer Genetics Program, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA. Matthew_Breen@ncsu.edu.
authors.forename: Matthew
authors.initials: M
authors.lastname: Breen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor / Q000145:classification; D020732:Cytogenetic Analysis; D004285:Dogs; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D016678:Genome / Q000235:genetics*; D008223:Lymphoma / Q000145:classification* / Q000503:physiopathology