title: RNA-seq Analysis of Overexpressing Ovine AANAT Gene of Melatonin Biosynthesis in Switchgrass.
abstract: Melatonin serves important functions in the promotion of growth and anti-stress regulation by efficient radical scavenging and regulation of antioxidant enzyme activity in various plants. To investigate its regulatory roles and metabolism pathways, the transcriptomic profile of overexpressing the ovine arylalkylamine N-acetyltransferase (oAANAT) gene, encoding the penultimate enzyme in melatonin biosynthesis, was compared with empty vector control using RNA-seq in switchgrass, a model plant of cellulosic ethanol conversion. The 85.22 million high quality reads that were assembled into 135,684 unigenes were generated by Illumina sequencing for transgenic oAANAT switchgrass with an average sequence length of 716 bp. A total of 946 differentially expression genes in transgenic line comparing to control switchgrass, including 737 up-regulated and 209 down-regulated genes, were mainly enriched with two main functional patterns of melatonin identifying by gene ontology analysis: the growth regulator and stress tolerance. Furthermore, KEGG maps indicated that the biosynthetic pathways of secondary metabolite (phenylpropanoids, flavonoids, steroids, stilbenoid, diarylheptanoid, and gingerol) and signaling pathways (MAPK signaling pathway, estrogen signaling pathway) were involved in melatonin metabolism. This study substantially expands the transcriptome information for switchgrass and provides valuable clues for identifying candidate genes involved in melatonin biosynthesis and elucidating the mechanism of melatonin metabolism.
authors.affiliation: College of Animal Science and Technology, China Agricultural University Beijing, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Yuan
authors.affiliation: College of Animal Science and Technology, China Agricultural UniversityBeijing, China; College of Agriculture, China Agricultural UniversityBeijing, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, China Agricultural University Beijing, China.
authors.forename: Sijia
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, China Agricultural University Beijing, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Guan
authors.affiliation: College of Animal Science and Technology, China Agricultural University Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Cui
authors.affiliation: College of Animal Science and Technology, China Agricultural University Beijing, China.
authors.forename: Danyang
authors.initials: D
authors.lastname: Tian
authors.affiliation: College of Animal Science and Technology, China Agricultural UniversityBeijing, China; Beijing Key Laboratory for Grassland Science, China Agricultural UniversityBeijing, China; National Energy R&D Center for BiomassBeijing, China.
authors.forename: Yunwei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, China Agricultural UniversityBeijing, China; Beijing Sure Academy of BiosciencesBeijing, China.
authors.forename: Fuyu
authors.initials: F
authors.lastname: Yang
keywords: RNA-seq; melatonin; oAANAT; overexpression; switchgrass