title: Organoid Culture of Human Cancer Stem Cells.
abstract: Organoid culture is a three-dimensional culture method that enables ex vivo analysis of stem cell behavior and differentiation. This method is also applicable to the studies on stem cell characters of human cancer stem cells. The components of organoid culture include Matrigel® and a culture medium containing growth factor cocktails that mimic the microenvironments of organ stem cell niches. Here, we describe the basic methods for the organoid culture of dissociated or FACS-sorted human cancer stem cells. Then, we introduce a method to dissociate the organoids for serial passage and propagation.
authors.affiliation: Division of Molecular and Cellular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan. yshimono@med.kobe-u.ac.jp.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shimono
authors.affiliation: Division of Molecular and Cellular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Mukohyama
authors.affiliation: Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA, USA.
authors.forename: Taichi
authors.initials: T
authors.lastname: Isobe
authors.affiliation: Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA.
authors.forename: Darius M
authors.initials: DM
authors.lastname: Johnston
authors.affiliation: Department of Pathology and Cell Biology, Columbia University, New York, NY, USA.
authors.forename: Piero
authors.initials: P
authors.lastname: Dalerba
authors.affiliation: Division of Molecular and Cellular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D006801:Humans; D009369:Neoplasms / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000473:pathology*; D009940:Organoids / Q000473:pathology*; D055153:Stem Cell Niche; D014407:Tumor Cells, Cultured
keywords: Cancer stem cells; Clonogenicity; Growth factor cocktails; Matrigel; Organoids; Passage