title: The garlic NF-YC gene, AsNF-YC8, positively regulates non-ionic hyperosmotic stress tolerance in tobacco.
abstract: To investigate the relationship between nuclear factor Y (NF-Y) and stress tolerance in garlic, we cloned a NF-Y family gene AsNF-YC8 from garlic, which was largely upregulated at dehydrate stage. Expression pattern analyses in garlic revealed that AsNF-YC8 is induced through abscisic acid (ABA) and abiotic stresses, such as NaCl and PEG. Compared with wild-type plants, the overexpressing-AsNF-YC8 transgenic tobacco plants showed higher seed germination rates, longer root length and better plant growth under salt and drought stresses. Under drought stress, the transgenic plants maintained higher relative water content (RWC), net photosynthesis, lower levels of malondialdehyde (MDA), and less ion leakage (IL) than wild-type control plants. These results indicate the high tolerance of the transgenic plants to drought stress compared to the WT. The transgenic tobacco lines accumulated less reactive oxygen species (ROS) and exhibited higher antioxidative enzyme activities compared with wild-type (WT) plants under drought stress, which suggested that the overexpression of AsNF-YC8 improves the antioxidant defense system by regulating the activities of these antioxidant enzymes, which in turn protect transgenic lines against drought stress. These results suggest that AsNF-YC8 plays an important role in tolerance to drought and salt stresses.
authors.affiliation: State Key Laboratory of Crop Biology, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China.
authors.forename: Xiudong
authors.initials: X
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Crop Biology, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Lian
authors.affiliation: State Key Laboratory of Crop Biology, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China.
authors.forename: Xingchen
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China.
authors.forename: Shumei
authors.initials: S
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Crop Biology, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, 271018, People's Republic of China. sqliu@sdau.edu.cn.
authors.forename: Shiqi
authors.initials: S
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D023081:CCAAT-Binding Factor / Q000235:genetics*; D003001:Cloning, Molecular; D055864:Droughts; D005737:Garlic / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018525:Germination / Q000235:genetics; D008315:Malondialdehyde / Q000378:metabolism; D009997:Osmotic Pressure / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D055051:Salt-Tolerant Plants / Q000235:genetics / Q000502:physiology*; D036226:Seedlings / Q000378:metabolism; D012965:Sodium Chloride; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*; D014170:Transformation, Genetic
chemical_list: D000975:Antioxidants; D023081:CCAAT-Binding Factor; D017382:Reactive Oxygen Species; C402244:nuclear factor Y; D012965:Sodium Chloride; D008315:Malondialdehyde
keywords: Garlic; NF-YC; Stress; Tobacco; Transgenic