title: Solution structure of protein synthesis initiation factor 1 from Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa is an opportunistic bacterial pathogen and a primary cause of nosocomial infection in humans. The rate of antibiotic resistance in P. aeruginosa is increasing worldwide leading to an unmet need for discovery of new chemical compounds distinctly different from present antimicrobials. Protein synthesis is an essential metabolic process and a validated target for the development of new antibiotics. Initiation factor 1 from P. aeruginosa (Pa-IF1) is the smallest of the three initiation factors that act to establish the 30S initiation complex during initiation of protein biosynthesis. Here we report the characterization and solution NMR structure of Pa-IF1. Pa-IF1 consists of a five-stranded β-sheet with an unusual extended β-strand at the C-terminus and one short α-helix arranged in the sequential order β1-β2-β3-α1-β4-β5. The structure adopts a typical β-barrel fold and contains an oligomer-binding motif. A cluster of basic residues (K39, R41, K42, K64, R66, R70, and R72) located on the surface of strands β4 and β5 near the short α-helix may compose the binding interface with the 30S subunit.
authors.affiliation: Department of Chemistry, The University of Texas Rio Grande Valley, Edinburg, Texas.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Chemistry, The University of Texas Rio Grande Valley, Edinburg, Texas.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Bernal
authors.affiliation: Department of Chemistry, The University of Texas Rio Grande Valley, Edinburg, Texas.
authors.forename: James M
authors.initials: JM
authors.lastname: Bullard
authors.affiliation: Department of Chemistry, The University of Texas Rio Grande Valley, Edinburg, Texas.
authors.forename: Yonghong
authors.identifier: 0000-0002-7305-9295
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D039861:Prokaryotic Initiation Factor-1 / Q000737:chemistry*; D000072417:Protein Domains; D011550:Pseudomonas aeruginosa / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D039861:Prokaryotic Initiation Factor-1
keywords: NMR structure; Pseudomonas aeruginosa; translation initiation factor 1; β-barrel motif