title: Probiotic mixture supplementation in the preventive management of trinitrobenzenesulfonic acid-induced inflammation in a murine model.
abstract: Inflammatory bowel diseases (IBD) are characterized by inflammatory conditions of the intestine. Probiotic bacteria (PB) can have beneficial effects in several gastrointestinal disorders. The objectives of this study were: (i) to provide an acute experimental IBD model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) in CD-1 mice, and (ii) to assess the preventive effects of Citogenex (Lactobacillus casei and Bifidobacterum lactis) supplementation on intestinal tissues and microbiota. Mice were inoculated intrarectally with saline, ethanol or different TNBS solutions. 1%TNBS induced clinical signs of colitis (P less than 0.01) and histological damage (P less than 0.01). Based on these results, mice were pre-treated with Citogenex or saline for 1, 2 or 3 weeks before 1%TNBS treatment. Probiotic pre-treatment determined a reduction of clinical signs (P less than 0.05), histological alterations of colitis (P less than 0.05) and increased beneficial bacteria (P less than 0.05). This study confirms that TNBS-induced colitis in CD-1 mice is useful for studying the mechanisms involved in IBD pathogenesis, and pre-treatment with Citogenex prevents the intestinal damage induced by TNBS.
authors.affiliation: Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Traina
authors.affiliation: Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Menchetti
authors.affiliation: Department of Legal Sciences of the Society and Sports, University of Palermo, Palermo, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Rappa
authors.affiliation: Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Casagrande-Proietti
authors.affiliation: Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
authors.forename: O
authors.initials: O
authors.lastname: Barbato
authors.affiliation: Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Leonardi
authors.affiliation: Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Carini
authors.affiliation: Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Piro
authors.affiliation: Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Brecchia
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D000069982:Bifidobacterium animalis; D003092:Colitis / Q000139:chemically induced / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D003106:Colon / Q000382:microbiology / Q000473:pathology; D004195:Disease Models, Animal; D000069196:Gastrointestinal Microbiome; D015212:Inflammatory Bowel Diseases / Q000517:prevention & control*; D007780:Lacticaseibacillus casei; D008297:Male; D051379:Mice; D019936:Probiotics / Q000627:therapeutic use*; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity
chemical_list: D014302:Trinitrobenzenesulfonic Acid