title: Characterizing the nuclear proteome of Paracoccidioides spp.
abstract: Paracoccidioidomycosis is an endemic disease in Latin America, caused by thermo dimorphic fungi of the genus Paracoccidioides. Although previous proteome analyses of Paracoccidioides spp. have been carried out, the nuclear subproteome of this pathogen has not been described. In this way, we aimed to characterize the nuclear proteome of Paracoccidioides species, in the yeast form. For that, yeast cells were disrupted and submitted to cell fractionation. The purity of the nuclear fraction was confirmed by fluorescence and electron microscopy. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) allowed the identification of 867 proteins. In order to support our enrichment method for nuclear proteins, bioinformatics analysis were applied that allowed the identification of 281 proteins with nuclear localization. The analysis revealed proteins related to DNA maintenance, gene expression, synthesis and processing of messenger and ribosomal RNAs, likewise proteins of nuclear-cytoplasmic traffic. It was also possible to detect some proteins that are poorly expressed, like transcription factors involved in important roles such as resistance to abiotic stress, sporulation, cellular growth and DNA and chromatin maintenance. This is the first descriptive nuclear proteome of Paracoccidioides spp. that can be useful as an important platform base for fungi-specific nuclear processes.
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de Goiás, Goiânia, Goiás, 74690-900, Brazil.
authors.forename: Lucas Nojosa
authors.initials: LN
authors.lastname: Oliveira
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de Goiás, Goiânia, Goiás, 74690-900, Brazil; Escola de Engenharia, Pontifícia Universidade Católica de Goiás, Goiânia, Goiás, 74605-010, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Casaletti
authors.affiliation: Laboratório de Microscopia, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, Distrito Federal, 70910-900, Brazil.
authors.forename: Sônia Nair
authors.initials: SN
authors.lastname: Báo
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de Goiás, Goiânia, Goiás, 74690-900, Brazil.
authors.forename: Clayton Luiz
authors.initials: CL
authors.lastname: Borges
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de Goiás, Goiânia, Goiás, 74690-900, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: de Sousa Lima
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de Goiás, Goiânia, Goiás, 74690-900, Brazil. Electronic address: cmasoares@gmail.com.
authors.forename: Célia Maria
authors.initials: CM
authors.lastname: de Almeida Soares
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010228:Paracoccidioides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D020543:Proteome / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D005656:Fungal Proteins; D020543:Proteome
keywords: Bioinformatic analysis; Cell biology; MS(E) proteomics; Nuclear proteins and paracoccidioidomycosis