title: Gag-Specific CD4 T Cell Proliferation, Plasmacytoid Dendritic Cells, and Ethnicity in Perinatally HIV-1-Infected Youths: The ANRS-EP38-IMMIP Study.
abstract: In perinatally HIV-1-infected youths living in France, we previously reported that Gag-specific CD4 and CD8 T cell proliferation is more frequently detected in patients of black ethnicity than in those of other ethnicities. We observed that black patients had higher levels of dendritic cells (DCs) than other patients. We aimed at studying the association of DC levels with Gag-specific T cell proliferation. The ANRS-EP38-IMMIP study is an observational study of youths aged between 15 and 24 years who were perinatally infected with HIV. A single blood sample was drawn for virological and immunological assays. Data from cART-treated 53 youths with undetectable plasma HIV RNA were analyzed. Gag-specific T cell proliferation was assessed by using a CFSE-based test. Peripheral blood myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs) were phenotyped by flow cytometry. Plasma markers were quantified by ELISA or multiplex assays. Logistic regression was used for univariate and multivariate analyses. Patients with Gag-specific CD4 T cell proliferative responses had significantly higher percentages and absolute counts of mDCs and pDCs in the peripheral blood than nonresponding patients. Gag-specific CD4 and CD8 T cell proliferation was associated with lower plasma sCD14 levels. Plasma levels of IFN-α, TRAIL, and chemokines involved in T cell migration to secondary lymphoid organs were not associated with T cell proliferation. Multivariate analysis confirmed the association between Gag-specific CD4 T cell proliferation and pDC levels. In conclusion, DC levels are a robust correlate of the presence of Gag-specific T cell proliferation in successfully treated youths.
authors.affiliation: 1 Unité Cytokines et Inflammation, Institut Pasteur , Paris, France .
authors.forename: Daniel
authors.initials: D
authors.lastname: Scott-Algara
authors.affiliation: 2 INSERM U1018 , CESP, Le Kremlin-Bicêtre, France .|3 Université Paris-Sud , Le Kremlin-Bicêtre, France .
authors.forename: Josiane
authors.initials: J
authors.lastname: Warszawski
authors.affiliation: 2 INSERM U1018 , CESP, Le Kremlin-Bicêtre, France .
authors.forename: Jérôme Le
authors.initials: JL
authors.lastname: Chenadec
authors.affiliation: 4 Groupe Mécanismes de l'Hérédité Epigénétique, Institut Pasteur , Paris, France .
authors.forename: Céline
authors.initials: C
authors.lastname: Didier
authors.affiliation: 5 Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur , Paris, France .|6 UMR 3569, CNRS , Paris, France .
authors.forename: Thomas
authors.initials: T
authors.lastname: Montange
authors.affiliation: 7 EA7327, Faculté de Médecine, Université Paris Descartes , Sorbonne Paris Cité, Paris, France .|8 AP-HP, Centre de Diagnostic et de Thérapeutique , Hôpital de l'Hôtel-Dieu, Paris, France .
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Viard
authors.affiliation: 9 AP-HP, Service d'Hématologie et d'Oncologie Pédiatrique, Hôpital Trousseau , Paris, France .
authors.forename: Catherine
authors.initials: C
authors.lastname: Dollfus
authors.affiliation: 7 EA7327, Faculté de Médecine, Université Paris Descartes , Sorbonne Paris Cité, Paris, France .|10 AP-HP, Laboratoire de Virologie, Hôpital Necker-Enfants Malades , Paris, France .
authors.forename: Véronique
authors.initials: V
authors.lastname: Avettand-Fenoel
authors.affiliation: 7 EA7327, Faculté de Médecine, Université Paris Descartes , Sorbonne Paris Cité, Paris, France .|10 AP-HP, Laboratoire de Virologie, Hôpital Necker-Enfants Malades , Paris, France .
authors.forename: Christine
authors.initials: C
authors.lastname: Rouzioux
authors.affiliation: 11 AP-HP, Unité Immunologie et Hématologie Pédiatrique, Hôpital Necker-Enfants Malades , Paris, France .
authors.forename: Stéphane
authors.initials: S
authors.lastname: Blanche
authors.affiliation: 5 Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur , Paris, France .|6 UMR 3569, CNRS , Paris, France .
authors.forename: Florence
authors.initials: F
authors.lastname: Buseyne
mesh_terms: D000293:Adolescent; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D003584:Cytological Techniques; D003713:Dendritic Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005602:France / Q000453:epidemiology; D015658:HIV Infections / Q000453:epidemiology / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D055815:Young Adult; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
chemical_list: D054301:gag Gene Products, Human Immunodeficiency Virus
keywords: CD4 T cells; T cell proliferation; dendritic cells; perinatal HIV infection; sCD14; youths