title: Low-grade inflammation markers in children and adolescents: Influence of anthropometric characteristics and CRP and IL6 polymorphisms.
abstract: Overweight and obesity are associated with chronic and subclinical inflammation due to an imbalance of inflammatory mediators. However, the association with gene polymorphism has been rarely studied in children. The aim of this study was to determine if serum concentrations of C-reactive protein (CRP) and interleukin-6 (IL-6) are related to the IL6 rs1800795, IL6 rs2069845 and CRP rs1205 polymorphisms (SNPs) according to body mass index (BMI) in a sample of children and adolescents. A cross-sectional study in 470 students between 7 and 17yearsof age of anthropometric characteristics, high sensitivity-CRP (Hs-CRP) and IL-6 levels and three SNPs genotyped. The prevalence ratio of hs-CRP>3mg/L in obese individuals was 4.15 (CI 2.43-7.06; p=0.01), and it was 1.91 (CI 1.03-3.55; p=0.03) in overweight individuals and 1.74 (CI 1.05-2.88 p=0.03) in females. Individuals with waist circumference (WC) and body fat percentage (BF%) alterations showed elevated levels of hs-CRP (p=4.3×10-5and p=5.3×10-6). The combination of any two anthropometric measurement increases CRP levels, especially combinations with obesity body mass index (BMI): BMI+WC and BMI+BF%. Among the overweight/obesity group, T allele carriers of CRP rs1205 showed lower levels of hs-CRP (0.5, IQR=0.3-1.8mg/L) than CC homozygotes (1.5, IQR=0.4-3.4mg/L, p=0.018). Additionally, considering subjects with two or three anthropometric alterations for CRP rs1205: rs1205 T allele carriers had lower levels of hs-CRP (0.7, IQR=0.3-2.7mg/L) than CC homozygotes (1.2, IQR=0.5-3.5mg/L, p=0.02). In conclusion, carriers of the rs1205/T allele with higher BMIs had lower levels of hs-CRP. Schoolchildren who were overweight/obese had higher levels of CRP and IL-6, whereas individuals with WC and BF% alterations had higher levels of CRP.
authors.affiliation: Post-Graduation Program in Health Promotion, University of Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS, Brazil. Electronic address: pamelaferreiratodendi@gmail.com.
authors.forename: P F
authors.initials: PF
authors.lastname: Todendi
authors.affiliation: Department of Biology and Pharmaceutical Sciences and the Post-Graduation Program in Health Promotion, University of Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS, Brazil. Electronic address: liapossuelo@unisc.br.
authors.forename: L G
authors.initials: LG
authors.lastname: Possuelo
authors.affiliation: Post-Graduation Program in Health Promotion, University of Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS, Brazil. Electronic address: elisaklinger@hotmail.com.
authors.forename: E I
authors.initials: EI
authors.lastname: Klinger
authors.affiliation: Department of Physical Education and Health and the Post-Graduation Program in Health Promotion, University of Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS, Brazil. Electronic address: cpreuter@hotmail.com.
authors.forename: C P
authors.initials: CP
authors.lastname: Reuter
authors.affiliation: Department of Physical Education and Health and the Post-Graduation Program in Health Promotion, University of Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS, Brazil. Electronic address: mburgos@unisc.br.
authors.forename: M S
authors.initials: MS
authors.lastname: Burgos
authors.affiliation: Laboratory of Genetic Toxicology, Federal University of Health Sciences of Porto Alegre - UFCSPA, Porto Alegre, RS, Brazil. Electronic address: dinjamoura@gmail.com.
authors.forename: D J
authors.initials: DJ
authors.lastname: Moura
authors.affiliation: Department of Basic Health Sciences, Federal University of Health Sciences of Porto Alegre - UFCSPA, Porto Alegre, RS, Brazil. Electronic address: e.mariluf@ufcspa.edu.br.
authors.forename: M
authors.initials: M
authors.lastname: Fiegenbaum
authors.affiliation: Department of Biology and Pharmaceutical Sciences and the Post-Graduation Program in Health Promotion, University of Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS, Brazil. Electronic address: avalim@unisc.br.
authors.forename: Andréia Rosane de Moura
authors.initials: AR
authors.lastname: Valim
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism; D002648:Child; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000235:genetics / Q000473:pathology; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics; D008297:Male; D009765:Obesity / Q000097:blood / Q000235:genetics / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D012737:Sex Factors; D055105:Waist Circumference
chemical_list: D015415:Biomarkers; C508600:IL6 protein, human; D015850:Interleukin-6; D002097:C-Reactive Protein
keywords: CRP; IL6; Inflammation; Obesity; Polymorphisms