title: Microbial community analysis using MiSeq sequencing in a novel configuration fluidized bed reactor for effective denitrification.
abstract: A novel configured fluidized bed reactor (FBR) with granular rubber as the fluidized media was operated without internal recirculation to achieve denitrification. This FBR could operate under a low hydraulic retention time (HRT) of 50min due to the low rubber media density and absence of recirculation. Synthetic nitrate-rich wastewater with a fixed nitrate (NO3--N) concentration and varying COD concentrations was fed into the FBR. The nitrate removal profile showed a rapid nitrate reduction at the bottom of the reactor with a high performance under the low HRT. Different microbial communities were identified using Illumina Miseq sequencing. The dominant microorganisms belonged to the Beta- and Gamma-proteobacteria classes and played important roles in nitrate reduction. Acidovorax was abundant at low COD: NO3--N ratios, while Rhizobium and Zoogloea were dominant at high COD: NO3--N ratios. The COD: NO3--N ratio strongly influenced the composition of the microbial community including the dominant species.
authors.affiliation: Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
authors.forename: Phatchariya
authors.initials: P
authors.lastname: Rungkitwatananukul
authors.affiliation: Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
authors.forename: Supanee
authors.initials: S
authors.lastname: Nomai
authors.affiliation: Department of Environmental Systems Engineering, Nagaoka University of Technology, 1603-1, Kami-tomioka, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Yuga
authors.initials: Y
authors.lastname: Hirakata
authors.affiliation: Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand. Electronic address: wiboonluk.p@chula.ac.th.
authors.forename: Wiboonluk
authors.initials: W
authors.lastname: Pungrasmi
authors.affiliation: Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
authors.forename: Chaiyaporn
authors.initials: C
authors.lastname: Puprasert
authors.affiliation: Department of Environmental Systems Engineering, Nagaoka University of Technology, 1603-1, Kami-tomioka, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Hatamoto
authors.affiliation: Department of Environmental Systems Engineering, Nagaoka University of Technology, 1603-1, Kami-tomioka, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamaguchi
mesh_terms: D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D058440:Denitrification; D004867:Equipment Design; D059013:Microbial Consortia / Q000235:genetics*; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014865:Waste Disposal, Fluid / Q000295:instrumentation* / Q000379:methods; D062065:Wastewater / Q000737:chemistry
chemical_list: D009566:Nitrates; D009584:Nitrogen; D062065:Wastewater
keywords: 16S rRNA gene sequencing; Denitrification; Fluidized bed reactor; Low COD to nitrate ratio; Microbial community analysis