title: Identification and clinical significance of an elevated level of serum aminoacylase-1 autoantibody in patients with hepatitis B virus-related liver cirrhosis.
abstract: Chronic hepatitis B (CHB) is prevalent worldwide and can develop into liver cirrhosis and liver carcinoma. Early discrimination of liver cirrhosis from chronic hepatitis is critical for effective treatment and optimal prognosis. The aim of the present study was to assess the diagnostic value of a panel of cellular proteins that can be recognized by autoantibodies in patient serum for hepatitis B virus (HBV)‑related liver cirrhosis. Twenty‑two candidate autoantigens screened using a serum proteomics assay in our previous study were assessed retrospectively in 443 participants, comprising 89 patients with HBV‑related liver cirrhosis, 89 patients with CHB, and 265 healthy controls. The levels of autoantibodies against the candidate autoantigens were measured by protein microarrays containing the candidate antigen proteins. Receiver operating characteristic (ROC) curves were used to calculate the diagnostic accuracy. The present study determined that seven of the 22 candidate autoantibodies differed significantly in serum level between HBV‑related liver cirrhosis and CHB (P<0.0001), with area under curve (AUC) values >0.7. The seven autoantibodies recognized aminoacylase‑1 (ACY1), histidine triad nucleotide‑binding protein 1, insulin‑like growth factor 2 mRNA‑binding protein 2, heat shock 70 kDa protein 6, peroxiredoxin 3, apoptosis‑inducing factor and regucalcin. Among these, the ACY1 autoantibody had the highest value for discriminating HBV‑related liver cirrhosis from CHB, with an AUC value of 0.872 (95% confidence interval: 0.810‑0.934, P<0.0001), sensitivity of 77.3% and specificity of 85.0%. In conclusion, with the elevated level in the disease progression of CHB, ACY1 autoantibody may be a valuable serum biomarker for discriminating HBV‑related liver cirrhosis from CHB.
authors.affiliation: Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: He
authors.affiliation: Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Department of Obstetrics, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, P.R. China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Obstetrics, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, P.R. China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Oncology Minimally Invasive Interventional Radiology, Beijing You‑an Hospital, Capital Medical University, Beijing 100069, P.R. China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Long
authors.affiliation: Department of Hepatopancreatobiliary and Splenic Medicine, Affiliated Hospital of Medical College of Chinese People's Armed Police Force, Tianjin 300192, P.R. China.
authors.forename: Hai
authors.initials: H
authors.lastname: Li
authors.affiliation: Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Bei
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Biotechnology Center, Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China.
authors.forename: Suhong
authors.initials: S
authors.lastname: Chen
authors.affiliation: Biotechnology Center, Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China.
authors.forename: Qiqi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Hongyi
authors.initials: H
authors.lastname: Li
authors.affiliation: Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wang
authors.affiliation: Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Ou
authors.affiliation: Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D000581:Amidohydrolases / Q000097:blood* / Q000276:immunology; D001323:Autoantibodies / Q000097:blood* / Q000302:isolation & purification; D003937:Diagnosis, Differential; D018450:Disease Progression; D005260:Female; D006515:Hepatitis B virus / Q000276:immunology / Q000472:pathogenicity; D019694:Hepatitis B, Chronic / Q000097:blood* / Q000276:immunology / Q000473:pathology; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000276:immunology / Q000821:virology; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011379:Prognosis
chemical_list: D001323:Autoantibodies; D000581:Amidohydrolases; C019672:aminoacylase I