title: Photoinduced formation of thiols in human hair.
abstract: Raman, scanning electron, and optical microscopy of hair and spectrophotometry of soluble hair proteins are used to study the effect of UV-vis radiation on white hair. The samples of a healthy subject are irradiated using a mercury lamp and compared with non-irradiated (control) hair. The cuticle damage with partial exfoliation is revealed with the aid of SEM and optical microscopy of semifine sections. Gel filtration chromatography shows that the molecular weight of soluble proteins ranges from 5 to 7kDa. Absorption spectroscopy proves an increase in amount of thiols in a heavier fraction of the soluble proteins of irradiated samples under study. Raman data indicate a decrease in the amount of SS and CS bonds in cystines and an increase in the amount of SH bonds due to irradiation. Such changes are more pronounced in peripheral regions of hair. Conformational changes of hair keratins presumably related to the cleavage of disulfide bonds, follow from variations in amide I and low-frequency Raman bands. An increase in the content of thiols in proteins revealed by both photometric data on soluble proteins and Raman microspectroscopy of hair cuts can be used to develop a protocol of the analysis of photoinduced hair modification.
authors.affiliation: Federal Scientific Clinical Centre of Physical Chemical Medicine, Malaya Pyrogovskaya 1-a, Moscow 119435, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Fedorkova
authors.affiliation: Faculty of Physics and International Laser Center, Lomonosov Moscow State University, Leninskiye Gory 1, bldg. 2, Moscow 119991, Russia. Electronic address: brandt@physics.msu.ru.
authors.forename: N N
authors.initials: NN
authors.lastname: Brandt
authors.affiliation: Faculty of Physics and International Laser Center, Lomonosov Moscow State University, Leninskiye Gory 1, bldg. 2, Moscow 119991, Russia.
authors.forename: A Yu
authors.initials: AY
authors.lastname: Chikishev
authors.affiliation: Federal Scientific Clinical Centre of Physical Chemical Medicine, Malaya Pyrogovskaya 1-a, Moscow 119435, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Smolina
authors.affiliation: Faculty of Chemistry, Lomonosov Moscow State University, Leninskiye Gory 1, bldg. 3, Moscow 119991, Russia.
authors.forename: N G
authors.initials: NG
authors.lastname: Balabushevich
authors.affiliation: Federal Scientific Clinical Centre of Physical Chemical Medicine, Malaya Pyrogovskaya 1-a, Moscow 119435, Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Gusev
authors.affiliation: Pirogov Russian National Research Medical University, Ostrovitianova 1, Moscow 117997, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Lipatova
authors.affiliation: Peoples' Friendship University of Russia, Miklukho-Maklaya 8, Moscow 117198, Russia.
authors.forename: V M
authors.initials: VM
authors.lastname: Botchey
authors.affiliation: Federal Scientific Clinical Centre of Physical Chemical Medicine, Malaya Pyrogovskaya 1-a, Moscow 119435, Russia.
authors.forename: G E
authors.initials: GE
authors.lastname: Dobretsov
authors.affiliation: Federal Scientific Clinical Centre of Physical Chemical Medicine, Malaya Pyrogovskaya 1-a, Moscow 119435, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Mikhalchik
mesh_terms: D006197:Hair / Q000737:chemistry / Q000528:radiation effects*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D013059:Spectrum Analysis, Raman; D013438:Sulfhydryl Compounds / Q000032:analysis*; D014466:Ultraviolet Rays
chemical_list: D013438:Sulfhydryl Compounds
keywords: Disulfide bonds; Hair keratins; Photodamage; Photodegradation; Raman microspectroscopy; Sulfhydryl groups; Thiols