title: Study of Splicing Factor, Proline- and Glutamine-rich by Proteomic Techniques in Human Malignant and Nonmalignant Cell Lines.
abstract: Splicing factor, proline- and glutamine-rich protein (SFPQ), was identified in eight human cultivated cell lines by proteomic approaches. The cell proteins have been separated by means of two-dimensional gel electrophoresis in two modifications and identified by matrix-assisted laser desorption ionization mass spectrometry with further tandem mass spectrometry. The analysis of proteins from three human sarcomas cell lines (RD, U-2 OS and SK-UT-1B), three human renal adenocarcinomas cell lines (A-498, 769-P and OKP-GS), and two prostate adenocarcinomas cell lines (DU-145 and PC-3) revealed several electrophoretic isoforms of SFPQ protein. Differences between theoretical and experimental molecular masses and isoelectric points of SFPQ protein have been observed. Detailed investigation of SFPQ peptides by tandem mass spectrometry has detected new phosphorylation state of threonine residue in 168 position of SFPQ isoform in rhabdomyosarcoma cell line. Furthermore, SFPQ has not been identified during proteomic study of several nonmalignant cell lines, including cultured human mesenchymal stromal cells and myoblasts. However, SFPQ has been found in all malignant cell lines in high quantity. In particular, its fractions are abundant in sarcomas cell lines as opposed to nonmalignant mesenchymal cells. It is assumed that high quantity of SFPQ in sarcomas cell lines may affect tumorigenesis.
authors.affiliation: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences. 33, bld. 2 Leninsky Ave., Moscow 119071, Russian Federation.
authors.forename: Natalya V
authors.initials: NV
authors.lastname: Pashintseva
authors.forename: Sergey S
authors.initials: SS
authors.lastname: Shishkin
authors.forename: Kseniya V
authors.initials: KV
authors.lastname: Lisitskaya
authors.forename: Leonid I
authors.initials: LI
authors.lastname: Kovalev
authors.forename: Marina A
authors.initials: MA
authors.lastname: Kovaleva
authors.forename: Lidia S
authors.initials: LS
authors.lastname: Eryomina
authors.forename: Inna A
authors.initials: IA
authors.lastname: Kamenikhina
authors.forename: Lyudmila A
authors.initials: LA
authors.lastname: Novikova
authors.forename: Elchin G
authors.initials: EG
authors.lastname: Sadykhov
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D063646:Carcinogenesis / Q000473:pathology; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology*; D008297:Male; D000072670:PTB-Associated Splicing Factor / Q000032:analysis / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000473:pathology*; D020033:Protein Isoforms / Q000032:analysis / Q000378:metabolism; D040901:Proteomics; D012208:Rhabdomyosarcoma / Q000473:pathology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000072670:PTB-Associated Splicing Factor; D020033:Protein Isoforms