title: Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption.
abstract: Polyphenols exert a large range of beneficial effects in the prevention of age-related diseases. We sought to determine whether an extract of olive and grape seed standardized according to hydroxytyrosol (HT) and procyanidins (PCy) content, exerts preventive anti-osteoathritic effects. To this aim, we evaluated whether the HT/PCy mix could (i) have in vitro anti-inflammatory and chondroprotective actions, (ii) exert anti-osteoarthritis effects in two post-traumatic animal models and (iii) retain its bioactivity after oral administration. Anti-inflammatory and chondroprotective actions of HT/PCy were tested on primary cultured rabbit chondrocytes stimulated by interleukin-1 beta (IL-1β). The results showed that HT/PCy exerts anti-inflammatory and chondroprotective actions in vitro. The preventive effect of HT/PCy association was assessed in two animal models of post-traumatic OA in mice and rabbits. Diet supplementation with HT/PCy significantly decreased the severity of post-traumatic osteoarthritis in two complementary mice and rabbit models. The bioavailability and bioactivity was evaluated following gavage with HT/PCy in rabbits. Regular metabolites from HT/PCy extract were found in sera from rabbits following oral intake. Finally, sera from rabbits force-fed with HT/PCy conserved anti-IL-1β effect, suggesting the bioactivity of this extract. To conclude, HT/PCy extract may be of clinical significance for the preventive treatment of osteoarthritis.
authors.affiliation: INSERM, U791, LIOAD, Nantes, F-44042, France.|Union Grap'Sud, Cruviers-Lascours, F-30360, France.|Université de Nantes, UMR-S 791, LIOAD, UFR Odontologie, Nantes, F-44042, France.
authors.forename: Elsa
authors.initials: E
authors.lastname: Mével
authors.affiliation: INSERM, U791, LIOAD, Nantes, F-44042, France.|Université de Nantes, UMR-S 791, LIOAD, UFR Odontologie, Nantes, F-44042, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Merceron
authors.affiliation: INSERM, U791, LIOAD, Nantes, F-44042, France.|Université de Nantes, UMR-S 791, LIOAD, UFR Odontologie, Nantes, F-44042, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Vinatier
authors.affiliation: Université de Bordeaux, ISVV, EA 3675 GESVAB, Villenave d'Ornon, F- 33883, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Krisa
authors.affiliation: Université de Bordeaux, ISVV, EA 3675 GESVAB, Villenave d'Ornon, F- 33883, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Richard
authors.affiliation: INSERM, U791, LIOAD, Nantes, F-44042, France.|Université de Nantes, UMR-S 791, LIOAD, UFR Odontologie, Nantes, F-44042, France.
authors.forename: Martial
authors.initials: M
authors.lastname: Masson
authors.affiliation: INSERM, U791, LIOAD, Nantes, F-44042, France.|Université de Nantes, UMR-S 791, LIOAD, UFR Odontologie, Nantes, F-44042, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Lesoeur
authors.affiliation: INSERM, U791, LIOAD, Nantes, F-44042, France.|Université de Nantes, UMR-S 791, LIOAD, UFR Odontologie, Nantes, F-44042, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Hivernaud
authors.affiliation: INSERM, U791, LIOAD, Nantes, F-44042, France.|Université de Nantes, UMR-S 791, LIOAD, UFR Odontologie, Nantes, F-44042, France.|ONIRIS, UMR-S 791, LIOAD, Nantes, F-44307, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Gauthier
authors.affiliation: Cancers animaux, Modèle pour la recherche en Oncologie Comparée (AMaROC), Nantes, F-44307, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Abadie
authors.affiliation: INSERM, UMRS 938, Metabolism and age-related joint diseases, Université Paris 6 Pierre &Marie Curie, Paris, F-75252 France.
authors.forename: Geoffroy
authors.initials: G
authors.lastname: Nourissat
authors.affiliation: INSERM, UMRS 938, Metabolism and age-related joint diseases, Université Paris 6 Pierre &Marie Curie, Paris, F-75252 France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Houard
authors.affiliation: Clermont Université, Unité de Nutrition Humaine, Clermont-Ferrand, F- 63122, France.|INRA, UMR 1019, UNH, CRNH Auvergne, Clermont-Ferrand, F- 63122, France.
authors.forename: Yohann
authors.initials: Y
authors.lastname: Wittrant
authors.affiliation: Union Grap'Sud, Cruviers-Lascours, F-30360, France.
authors.forename: Nelly
authors.initials: N
authors.lastname: Urban
authors.affiliation: INSERM, U791, LIOAD, Nantes, F-44042, France.|Université de Nantes, UMR-S 791, LIOAD, UFR Odontologie, Nantes, F-44042, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Beck
authors.affiliation: INSERM, U791, LIOAD, Nantes, F-44042, France.|Université de Nantes, UMR-S 791, LIOAD, UFR Odontologie, Nantes, F-44042, France.|CHU Nantes, PHU 4 OTONN, Nantes, F-44042, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Guicheux
mesh_terms: D000284:Administration, Oral; D000818:Animals; D016118:Anterior Cruciate Ligament / Q000187:drug effects / Q000601:surgery; D044946:Biflavonoids / Q000494:pharmacology / Q000627:therapeutic use; D002392:Catechin / Q000494:pharmacology / Q000627:therapeutic use; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004032:Diet; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D056604:Grape Seed Extract / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D055442:Metabolome; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D031658:Olea / Q000737:chemistry*; D010003:Osteoarthritis / Q000097:blood / Q000188:drug therapy* / Q000209:etiology / Q000517:prevention & control*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D044945:Proanthocyanidins / Q000494:pharmacology / Q000627:therapeutic use; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D014947:Wounds and Injuries / Q000150:complications*
chemical_list: D044946:Biflavonoids; D056604:Grape Seed Extract; D053583:Interleukin-1beta; D044945:Proanthocyanidins; D012333:RNA, Messenger; C005975:3,4-dihydroxyphenylethanol; D009569:Nitric Oxide; C017674:procyanidin; D002392:Catechin; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D053509:Matrix Metalloproteinase 13; D015232:Dinoprostone; D010626:Phenylethyl Alcohol