title: New electroantennography method on a marine shrimp in water.
abstract: Antennular chemoreception in aquatic decapods is well studied via the recording of single chemoreceptor neuron activity in the antennule, but global responses of the antennule (or antennae in insects) by electroantennography (EAG) has so far been mainly restricted to aerial conditions. We present here a well-established underwater EAG method to record the global antennule activity in the marine shrimp Palaemon elegans in natural (aqueous) conditions. EAG responses to food extracts, recorded as net positive deviations of the baseline, are reproducible, dose-dependent and exhibit sensory adaptation. This new EAG method opens a large field of possibilities for studying in vivo antennular chemoreception in aquatic decapods, in a global approach to supplement current, more specific techniques.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, MNHN, CNRS, IRD, UCBN, UAG, Unité de Biologie des organismes et écosystèmes aquatiques (BOREA, UMR 7208), Equipe Adaptations aux Milieux Extrêmes, 7 Quai Saint-Bernard, Bâtiment A, Paris 75005, France.|iEES-Paris, Department of Sensory Ecology, INRA, Route de Saint-Cyr, Versailles 78026, France.
authors.forename: Julia
authors.initials: J
authors.lastname: Machon
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, MNHN, CNRS, IRD, UCBN, UAG, Unité de Biologie des organismes et écosystèmes aquatiques (BOREA, UMR 7208), Equipe Adaptations aux Milieux Extrêmes, 7 Quai Saint-Bernard, Bâtiment A, Paris 75005, France.
authors.forename: Juliette
authors.initials: J
authors.lastname: Ravaux
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, MNHN, CNRS, IRD, UCBN, UAG, Unité de Biologie des organismes et écosystèmes aquatiques (BOREA, UMR 7208), Equipe Adaptations aux Milieux Extrêmes, 7 Quai Saint-Bernard, Bâtiment A, Paris 75005, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Zbinden
authors.affiliation: iEES-Paris, Department of Sensory Ecology, INRA, Route de Saint-Cyr, Versailles 78026, France philippe.lucas@inra.fr.
authors.forename: Philippe
authors.identifier: 0000-0003-2166-8248
authors.initials: P
authors.lastname: Lucas
mesh_terms: D000818:Animals; D002628:Chemoreceptor Cells / Q000502:physiology*; D004594:Electrophysiology / Q000379:methods*; D033541:Palaemonidae / Q000502:physiology*; D058618:Sensilla / Q000502:physiology*
keywords: Antennule; Electroantennography; Olfaction; Shrimp