title: Transcriptome Analysis of HepG2 Cells Expressing ORF3 from Swine Hepatitis E Virus to Determine the Effects of ORF3 on Host Cells.
abstract: Hepatitis E virus- (HEV-) mediated hepatitis has become a global public health problem. An important regulatory protein of HEV, ORF3, influences multiple signal pathways in host cells. In this study, to investigate the function of ORF3 from the swine form of HEV (SHEV), high-throughput RNA-Seq-based screening was performed to identify the differentially expressed genes in ORF3-expressing HepG2 cells. The results were validated with quantitative real-time PCR and gene ontology was employed to assign differentially expressed genes to functional categories. The results indicated that, in the established ORF3-expressing HepG2 cells, the mRNA levels of CLDN6, YLPM1, APOC3, NLRP1, SCARA3, FGA, FGG, FGB, and FREM1 were upregulated, whereas the mRNA levels of SLC2A3, DKK1, BPIFB2, and PTGR1 were downregulated. The deregulated expression of CLDN6 and FREM1 might contribute to changes in integral membrane protein and basement membrane protein expression, expression changes for NLRP1 might affect the apoptosis of HepG2 cells, and the altered expression of APOC3, SCARA3, and DKK1 may affect lipid metabolism in HepG2 cells. In conclusion, ORF3 plays a functional role in virus-cell interactions by affecting the expression of integral membrane protein and basement membrane proteins and by altering the process of apoptosis and lipid metabolism in host cells. These findings provide important insight into the pathogenic mechanism of HEV.
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Kailian
authors.identifier: 0000-0003-1711-7793
authors.initials: K
authors.lastname: Xu
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Guo
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Tianjing
authors.initials: T
authors.lastname: Zhao
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Huapei
authors.initials: H
authors.lastname: Zhu
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Hanwei
authors.initials: H
authors.lastname: Jiao
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Qiaoyun
authors.initials: Q
authors.lastname: Shi
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Pang
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Yaying
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Li
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Peng
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Nie
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Ying
authors.identifier: 0000-0003-4861-5429
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Kebang
authors.initials: K
authors.lastname: Wu
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Li
authors.initials: L
authors.lastname: Du
authors.affiliation: Modern Agriculture Risk Warning and Prevention and Control Center of Hainan Province, Haikou 571100, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Cui
authors.affiliation: College of Animal Science, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia, Hohhot 010018, China.
authors.forename: Wenguang
authors.identifier: 0000-0002-3508-588X
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China.
authors.forename: Fengyang
authors.identifier: 0000-0002-2043-2703
authors.initials: F
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D005786:Gene Expression Regulation; D015967:Gene Expression Regulation, Viral; D056945:Hep G2 Cells / Q000821:virology*; D016752:Hepatitis E virus; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D050356:Lipid Metabolism; D008565:Membrane Proteins / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry; D015398:Signal Transduction; D013552:Swine; D059467:Transcriptome; D015854:Up-Regulation; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D008565:Membrane Proteins; C400973:ORF3 protein, Hepatitis E virus; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014764:Viral Proteins