title: Adeno-Associated Viral Vectors Transduce Mature Human Adipocytes in Three-Dimensional Slice Cultures.
abstract: Adipose tissue plays a pivotal role, both in the regulation of energy homeostasis and as an endocrine organ. Consequently, adipose tissue dysfunction is closely related to insulin resistance, morbid obesity, and metabolic syndrome. To study molecular mechanisms and to develop novel therapeutic strategies, techniques are required to genetically modify mature adipocytes. Here, we report on adeno-associated viral (AAV) vectors as a versatile tool to transduce human mature adipocytes in organotypic three-dimensional tissue cultures.
authors.affiliation: 1 Institute of Anatomy, University of Leipzig , Leipzig, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Kallendrusch
authors.affiliation: 2 Clinic for Orthopedic Surgery, Traumatology and Plastic Surgery, University Medicine Leipzig , Leipzig, Germany.
authors.forename: Nikolas
authors.initials: N
authors.lastname: Schopow
authors.affiliation: 3 Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig , Leipzig, Germany.
authors.forename: Sonja C
authors.initials: SC
authors.lastname: Stadler
authors.affiliation: 4 Institute of Experimental Hematology, Hannover Medical School , Hannover, Germany.|5 German Center for Infection Research , partner sites Bonn-Cologne and Hannover-Braunschweig, Germany.|6 Center for Molecular Medicine Cologne, University of Cologne , Cologne, Germany.
authors.forename: Hildegard
authors.initials: H
authors.lastname: Büning
authors.affiliation: 7 University Cancer Center Leipzig, University Medicine Leipzig , Leipzig, Germany .
authors.forename: Ulrich T
authors.initials: UT
authors.lastname: Hacker
mesh_terms: D017667:Adipocytes / Q000378:metabolism* / Q000821:virology; D018929:Cell Culture Techniques; D000229:Dependovirus / Q000235:genetics; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000627:therapeutic use*; D006801:Humans; D014161:Transduction, Genetic
keywords: adeno-associated viral vector; adipose tissue; gene transfer; slice culture