title: Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil.
abstract: Shrimp fisheries along the Brazilian coast have significant environmental impact due to high by-catch rates (21 kg per kilogram of shrimp). Typically discarded, by-catch contains many invertebrates that may host a great variety of bacterial genera, some of which may produce bioactive natural products with biotechnological applications. Therefore, to utilize by-catch that is usually discarded we explored the biotechnological potential of culturable bacteria of two abundant by-catch invertebrate species, the snail Olivancillaria urceus and the sea star Luidia senegalensis. Sediment from the collection area was also investigated. Utilizing multiple isolation approaches, 134 isolates were obtained from the invertebrates and sediment. Small-subunit rRNA (16S) gene sequencing revealed that the isolates belonged to Proteobacteria, Firmicutes and Actinobacteria phyla and were distributed among 28 genera. Several genera known for their capacity to produce bioactive natural products (Micromonospora, Streptomyces, Serinicoccus and Verrucosispora) were retrieved from the invertebrate samples. To query the bacterial isolates for their ability to produce bioactive metabolites, all strains were fermented and fermentation extracts profiled by UP LC-HRMS and tested for antimicrobial activity. Four strains exhibited antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus warneri.
authors.affiliation: Biosciences Institute, São Paulo State University - UNESP, Coastal Campus, Praça Infante Dom Henrique s/n, São Vicente, SP, P.O. Box 73601, 11380-972, Brazil.|Chemistry Institute, São Paulo State University - UNESP, Araraquara Campus. Prof. Francisco Degni, 55, Araraquara, SP, 14800-060, Brazil.
authors.forename: Marcelo M P
authors.identifier: http://orcid.org/0000-0002-4236-094X
authors.initials: MM
authors.lastname: Tangerina
authors.affiliation: Department of Chemistry, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada.
authors.forename: Hebelin
authors.initials: H
authors.lastname: Correa
authors.affiliation: Department of Chemistry, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada.|Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada.
authors.forename: Brad
authors.initials: B
authors.lastname: Haltli
authors.affiliation: Biosciences Institute, São Paulo State University - UNESP, Coastal Campus, Praça Infante Dom Henrique s/n, São Vicente, SP, P.O. Box 73601, 11380-972, Brazil.
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
authors.affiliation: Department of Chemistry, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada. rkerr@upei.ca.|Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada. rkerr@upei.ca.
authors.forename: Russell G
authors.initials: RG
authors.lastname: Kerr
mesh_terms: D000818:Animals; D001419:Bacteria / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D065132:Bioprospecting; D001938:Brazil; D019015:Geologic Sediments / Q000382:microbiology*; D007448:Invertebrates / Q000382:microbiology*; D010802:Phylogeny; D012908:Snails / Q000382:microbiology*; D013215:Starfish / Q000382:microbiology*; D014866:Waste Products / Q000032:analysis
chemical_list: D014866:Waste Products
keywords: 16S rDNA; Antimicrobial; Bacterial community; Luidia senegalensis; Marine bacteria; Olivancillaria urceus