title: A Prophage in Diabetic Foot Ulcer-Colonizing Staphylococcus aureus Impairs Invasiveness by Limiting Intracellular Growth.
abstract: The mechanisms that drive the transition from commensality to invasiveness in Staphylococcus aureus are poorly understood. We recently reported that >50% of S. aureus isolates from uninfected diabetic foot ulcers in French patients harbor a prophage, ROSA-like, that is absent from invasive isolates from diabetic foot infections, including osteomyelitis. Here we show that the ROSA-like insertion abolishes the ability of S. aureus to replicate within osteoblasts, the bone-forming cells, greatly reducing damage to infected cells. These results unravel an important mechanism by which particular S. aureus strains are maintained in a commensal state in diabetic foot ulcers.
authors.affiliation: INSERM U1111, International Center for Infectiology Research, Ecole Normale Supérieure de Lyon, CNRS UMR5308.|National Reference Center for Staphylococci, Institute of Infectious Agents, Lyon University Hospital.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Rasigade
authors.affiliation: INSERM U1047, Faculty of Medicine, University of Montpellier.|Department of Microbiology, University Hospital Carémeau, Nîmes, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Dunyach-Rémy
authors.affiliation: INSERM U1111, International Center for Infectiology Research, Ecole Normale Supérieure de Lyon, CNRS UMR5308.
authors.forename: Anaïs
authors.initials: A
authors.lastname: Sapin
authors.affiliation: INSERM U1047, Faculty of Medicine, University of Montpellier.
authors.forename: Nourredine
authors.initials: N
authors.lastname: Messad
authors.affiliation: INSERM U1111, International Center for Infectiology Research, Ecole Normale Supérieure de Lyon, CNRS UMR5308.
authors.forename: Sophie
authors.initials: S
authors.lastname: Trouillet-Assant
authors.affiliation: INSERM U1111, International Center for Infectiology Research, Ecole Normale Supérieure de Lyon, CNRS UMR5308.|National Reference Center for Staphylococci, Institute of Infectious Agents, Lyon University Hospital.
authors.forename: Céline
authors.initials: C
authors.lastname: Dupieux
authors.affiliation: INSERM U1047, Faculty of Medicine, University of Montpellier.|Department of Microbiology, University Hospital Carémeau, Nîmes, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Lavigne
authors.affiliation: INSERM U1111, International Center for Infectiology Research, Ecole Normale Supérieure de Lyon, CNRS UMR5308.|Institut des Sciences Pharmaceutiques et Biologiques de Lyon, University of Lyon.|National Reference Center for Staphylococci, Institute of Infectious Agents, Lyon University Hospital.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Laurent
mesh_terms: D017719:Diabetic Foot / Q000150:complications / Q000382:microbiology*; D005500:Follow-Up Studies; D005602:France; D006801:Humans; D008137:Longitudinal Studies; D010006:Osteoblasts / Q000382:microbiology*; D039002:Prophages / Q000254:growth & development*; D011446:Prospective Studies; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000254:growth & development* / Q000821:virology*; D014774:Virulence
keywords: ROSA phage; bacterial intracellular growth; bacterial invasion; diabetic foot infection; osteoblast; osteomyelitis