title: Lrrc75b is a novel negative regulator of C2C12 myogenic differentiation.
abstract: Many transcription factors and signaling molecules involved in the guidance of myogenic differentiation have been investigated in previous studies. However, the precise molecular mechanisms of myogenic differentiation remain largely unknown. In the present study, by performing a meta-analysis of C2C12 myogenic differentiation microarray data, we found that leucine-rich repeat-containing 75B (Lrrc75b), also known as AI646023, a molecule of unknown biological function, was downregulated during C2C12 myogenic differentiation. The knockdown of Lrrc75b using specific siRNA in C2C12 myoblasts markedly enhanced the expression of muscle-speciﬁc myogenin and increased myoblast fusion and the myotube diameter. By contrast, the adenovirus-mediated overexpression of Lrrc75b in C2C12 cells markedly inhibited myoblast differentiation accompanied by a decrease in myogenin expression. In addition, the phosphorylation of extracellular signal-regulated kinase 1/2 (Erk1/2) was suppressed in the cells in which Lrrc75b was silenced. Taken together, our results demonstrate that Lrrc75b is a novel suppressor of C2C12 myogenic differentiation by modulating myogenin and Erk1/2 signaling.
authors.affiliation: Department of Pharmacology, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China.
authors.forename: Yuechun
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Department of Pharmacology, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China.
authors.forename: Liyi
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China.
authors.forename: Zonggui
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Pharmacology, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China.
authors.forename: Xinguang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China.
authors.forename: Liao
authors.initials: L
authors.lastname: Cui
authors.affiliation: Department of Pharmacology, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Zuo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D015536:Down-Regulation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D051379:Mice; D008856:Microscopy, Fluorescence; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D018008:Myogenin / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C000615711:Lrrc75b protein, mouse; D009124:Muscle Proteins; D018008:Myogenin; D048049:Extracellular Signal-Regulated MAP Kinases