title: Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex.
abstract: The aim of the present study was to verify the long-term cytotoxic effects of the MTA Fillapex and to compare them with AH Plus. Dissolution rate and pH were also evaluated. Human osteoblast cells were incubated with elutes of fresh specimens from AH Plus and MTA Fillapex, and with elutes of the same specimens for 4 successive weeks. Elute's pH was evaluated at each time point. A multiparametric cell viability assay was performed. For dissolution rate, ISO methodology was used. The results were analyzed by one-way analysis of variance, complemented with the Tukey post-test (p<0.05). No significant difference was found among the materials when fresh mixed (p>0.05). After 1 week, AH Plus became non-cytotoxic on all three evaluated parameters. Conversely, MTA Fillapex remained severely and mildly cytotoxic over the entire experimental period (p<0.05). The dissolution rate of AH Plus was significantly lower than MTA Fillapex at all time points (p>0.05). The pH of AH Plus was significantly lower than MTA Fillapex at the second and third week (p<0.05). In the other tested time points no statistical difference was observed. In conclusion, MTA Fillapex remained cytotoxic after 4 weeks and its cytotoxicity may be related to the high dissolution rate of this material.
authors.affiliation: Department of Endodontics, UNIGRANRIO - Universidade do Grande Rio, Rio de Janeiro, RJ Brazil.
authors.forename: Emmanuel João Nogueira Leal da
authors.initials: EJ
authors.lastname: Silva
authors.affiliation: Department of Endodontics, UNIGRANRIO - Universidade do Grande Rio, Rio de Janeiro, RJ Brazil.
authors.forename: Thais
authors.initials: T
authors.lastname: Accorsi-Mendonça
authors.affiliation: Bioengineering Division, INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia, Duque de Caxias, RJ, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Pedrosa
authors.affiliation: Bioengineering Division, INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia, Duque de Caxias, RJ, Brazil.
authors.forename: José Mauro
authors.initials: JM
authors.lastname: Granjeiro
authors.affiliation: Department of Restorative Dentistry, Endodontic Division, Piracicaba Dental School, UNICAMP - Universidade de Campinas, Piracicaba, SP, Brazil.
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Zaia
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010006:Osteoblasts / Q000187:drug effects*; D012387:Root Canal Filling Materials; D012995:Solubility
chemical_list: D012387:Root Canal Filling Materials