title: Aberrant Expression of Long Non-Coding RNAs in Schizophrenia Patients.
abstract: BACKGROUND Dysfunction of long non-coding RNAs (lncRNAs) has been demonstrated to be involved in psychiatric diseases. However, the expression patterns and functions of the regulatory lncRNAs in schizophrenia (SZ) patients have rarely been systematically reported. MATERIAL AND METHODS The lncRNAs in peripheral blood mononuclear cells (PBMCs) were screened and compared between the SZ patients and demographically-matched healthy controls using microarray analysis, and then were validated by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) method. Three verified significantly dysregulated lncRNAs of PBMCs were selected and then measured in SZ patients before and after the antipsychotic treatment. SZ symptomatology improvement was measured by Positive And Negative Syndrome Scale (PANSS) scores. RESULTS One hundred and twenty-five lncRNAs were significantly differentially expressed in SZ patients compared with healthy controls, of which 62 were up-regulated and 63 were down-regulated. Concurrent with the significant decrease of the PANSS scores of patients after the treatment, the PBMC levels of lncRNA NONHSAT089447 and NONHSAT041499 were strikingly decreased (P<0.05). Down-regulation of PBMC expression of NONHSAT041499 was significantly correlated to the improvement of positive and activity symptoms of patients (r=-0.444 and -0.423, respectively, P<0.05, accounting for 16.9% and 15.1%, respectively), and was also significantly associated with better outcomes (odds ratio 2.325 for positive symptom and 12.340 for activity symptom). CONCLUSIONS LncRNA NONHSAT089447 and NONHSAT041499 might be involved in the pathogenesis and development of SZ, and the PBMC level of NONHSAT041499 is significantly associated with the treatment outcomes of SZ.
authors.affiliation: Department of Psychiatry and Psychology, Second Military Medical University, Shanghai, China (mainland).
authors.forename: Shengdong
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Psychiatry and Psychology, PingAn Health Cloud Company Ltd. of China, Shanghai, China (mainland).
authors.forename: Xinyang
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Rehabilitation, No. 102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, China (mainland).
authors.forename: Wei
authors.initials: W
authors.lastname: Niu
authors.affiliation: Prevention and Treatment Center for Psychological Diseases, No. 102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, China (mainland).
authors.forename: Lingming
authors.initials: L
authors.lastname: Kong
authors.affiliation: Prevention and Treatment Center for Psychological Diseases, No. 102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, China (mainland).
authors.forename: Mingjun
authors.initials: M
authors.lastname: He
authors.affiliation: , GoPath Diagnostic Laboratory Co. Ltd., Changzhou, Jiangsu, China (mainland).
authors.forename: Wanshuai
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Laboratory, No. 102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, China (mainland).
authors.forename: Aifang
authors.initials: A
authors.lastname: Zhong
authors.affiliation: , GoPath Diagnostic Laboratory Co. Ltd., Changzhou, Jiangsu, China (mainland).
authors.forename: Jim
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Psychiatry and Psychology, Second Military Medical University, Shanghai, China (mainland).
authors.forename: Liyi
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D016000:Cluster Analysis; D003710:Demography; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000097:blood / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012044:Regression Analysis; D015203:Reproducibility of Results; D012559:Schizophrenia / Q000097:blood / Q000235:genetics*; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D062085:RNA, Long Noncoding