title: First Record of Paramphistomes Fischoederius cobboldi and Paramphistomum epiclitum Detected in Bovine Rumen from a Local Market of Savannakhet Province, Lao PDR.
abstract: In the present study, we report on the occurrence of paramphistomes, Fischoederius cobboldi and Paramphistomum epiclitum, in Lao PDR with the basis of molecular data. Parasite materials were collected from bovines bred in Ban Lahanam area, Savannakhet Province, Lao PDR at Lahanam public market. Morphological observations indicated 2 different species of paramphistomes. The mitochondrial gene cox1 of the specimens was successfully amplified by PCR and DNA sequencing was carried out for diagnosis of 11 specimens. Pairwise alignment of cox1 sequences were performed and confirmed F. cobboldi and P. epiclitum infecting bovines in Laos. Although there were many limiting points, as the small number of worm samples, and the restricted access of the animal host materials, we confirmed for the first time that 2 species of paramphistomes, F. cobboldi and P. epiclitum, are distributed in Lao PDR. More studies are needed to confirm the paramphistome species present in Savannakhet and its hosts to clear the natural history of these parasites of ruminants in the region and measure the impact of this parasite infection in the life and health of the local people.
authors.affiliation: Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Surapol
authors.initials: S
authors.lastname: Sanguankiat
authors.affiliation: Department of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan.|Curso de Medicina, Universidade Federal do Tocantins, Palmas, Tocantins, Brasil.
authors.forename: Marcello Otake
authors.initials: MO
authors.lastname: Sato
authors.affiliation: Graduate School of Health Sciences, Niigata University, Niigata, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Sato
authors.affiliation: Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Wanna
authors.initials: W
authors.lastname: Maipanich
authors.affiliation: Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Tippayarat
authors.initials: T
authors.lastname: Yoonuan
authors.affiliation: Station of Malariology, Parasitology, and Entomology, Savannakhet Province, Lao PDR.
authors.forename: Tiengkham
authors.initials: T
authors.lastname: Pongvongsa
authors.affiliation: National Institute of Public Health, Ministry of Health, Lao PDR.
authors.forename: Boungnong
authors.initials: B
authors.lastname: Boupha
authors.affiliation: Department of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Chigusa
authors.affiliation: School of Tropical Medicine and Global Health, Nagasaki University, Nagasaki, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Moji
authors.affiliation: Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Jitra
authors.initials: J
authors.lastname: Waikagul
mesh_terms: D000818:Animals; D002417:Cattle; D003576:Electron Transport Complex IV / Q000235:genetics; D016266:Food Parasitology; D007812:Laos; D008853:Microscopy; D010250:Paramphistomatidae / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012417:Rumen / Q000469:parasitology*; D017422:Sequence Analysis, DNA
chemical_list: D003576:Electron Transport Complex IV
keywords: Fischoederius cobboldi; Laos; Paramphistomum epiclitum; amphistome; cattle; eco-health; helminth