title: Paper-Based Bipolar Electrode Electrochemiluminescence Switch for Label-Free and Sensitive Genetic Detection of Pathogenic Bacteria.
abstract: Genetic analysis is of great importance for the detection of pathogenic bacteria. Bacterial identification must become simpler, less expensive, and more rapid than the traditional methods. In this study, a low-cost, label-free, and wireless paper-based bipolar electrode electrochemiluminescence (pBPE-ECL) analysis system was constructed for the rapid and sensitive genetic detection of pathogenic bacteria. Wax-screen printing was used to form hydrophilic channels on filter paper, and a carbon ink-based bipolar electrode and driving electrodes were screen-printed into the channels. The "light-switch" molecule [Ru(phen)2dppz]2+(phen = 1,10-phenanthroline; dppz = dipyridophenazine) was used to intercalate into the base pairs of the double-stranded DNA PCR amplification products, and the complexs were then applied to the paper-based bipolar electrode to perform the ECL assays; the ECL of [Ru(phen)2dppz]2+is quenched in aqueous solution, but this molecule displays intense ECL when intercalated into double-stranded DNA. Under optimized experimental conditions, as little as 10 copies/μL of the genomic DNA of Listeria monocytogenes could be detected. Additionally, the system could also specifically distinguish Listeria monocytogenes from Salmonella, Escherichia coli O157:H7, and Staphylococcus aureus. This label-free, simple, and rapid method has potential in point-of-care applications for pathogen detection.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou 510631, China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Liu
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou 510631, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhou
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou 510631, China.
authors.forename: Weipeng
authors.initials: W
authors.lastname: Liu
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou 510631, China.
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Yang
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University , Guangzhou 510631, China.
authors.forename: Da
authors.initials: D
authors.lastname: Xing
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D019453:Escherichia coli O157 / Q000235:genetics; D007364:Intercalating Agents / Q000737:chemistry; D008089:Listeria monocytogenes / Q000235:genetics; D049449:Luminescence; D058889:Molecular Typing / Q000295:instrumentation / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry; D010209:Paper; D010618:Phenanthrolines / Q000737:chemistry; D019779:Salmonella enterica / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics
chemical_list: D007364:Intercalating Agents; D009942:Organometallic Compounds; D010618:Phenanthrolines; C084261:bis(phenanthroline)(dipyridophenazine)ruthenium(II); D002244:Carbon; D004247:DNA