title: Functional analysis of molecular interactions in synthetic auxin response circuits.
abstract: Auxin-regulated transcription pivots on the interaction between the AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) repressor proteins and the AUXIN RESPONSE FACTOR (ARF) transcription factors. Recent structural analyses of ARFs and Aux/IAAs have raised questions about the functional complexes driving auxin transcriptional responses. To parse the nature and significance of ARF-DNA and ARF-Aux/IAA interactions, we analyzed structure-guided variants of synthetic auxin response circuits in the budding yeast Saccharomyces cerevisiae Our analysis revealed that promoter architecture could specify ARF activity and that ARF19 required dimerization at two distinct domains for full transcriptional activation. In addition, monomeric Aux/IAAs were able to repress ARF activity in both yeast and plants. This systematic, quantitative structure-function analysis identified a minimal complex-comprising a single Aux/IAA repressing a pair of dimerized ARFs-sufficient for auxin-induced transcription.
authors.affiliation: Department of Biology, University of Washington, Seattle, WA 98195.
authors.forename: Edith
authors.identifier: 0000-0003-1567-9602
authors.initials: E
authors.lastname: Pierre-Jerome
authors.affiliation: Department of Biology, University of Washington, Seattle, WA 98195.
authors.forename: Britney L
authors.identifier: 0000-0002-1037-5717
authors.initials: BL
authors.lastname: Moss
authors.affiliation: Department of Biology, University of Washington, Seattle, WA 98195.
authors.forename: Amy
authors.initials: A
authors.lastname: Lanctot
authors.affiliation: Department of Biology, University of Washington, Seattle, WA 98195.
authors.forename: Amber
authors.initials: A
authors.lastname: Hageman
authors.affiliation: Department of Biology, University of Washington, Seattle, WA 98195 jn7@uw.edu.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Nemhauser
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D053263:Gene Regulatory Networks; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D055503:Protein Multimerization; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D019076:Transgenes
chemical_list: D007210:Indoleacetic Acids; D011993:Recombinant Fusion Proteins; C030737:indoleacetic acid
keywords: ARF; PB1; auxin signaling; synthetic circuits; transcription