title: Immunogenicity and antigenicity of a recombinant chimeric protein containing epitopes of poliovirus type 1.
abstract: To design a vaccine that simultaneously prevents both rotavirus (RV) and poliovirus (PV), a PV type 1 (PV1) chimeric protein using RV VP6 as a vector (VP6F) was constructed, expressed in Escherichia coli expression system and characterized by SDS-PAGE, Western blot, immunofluorescence assay and neutralization test. The results showed that the chimeric protein reacted with anti-VP6F and anti-PV1 antibodies and elicited production of serum antibodies against the chimeric protein in guinea pigs. Antibodies against the chimeric protein neutralized RV Wa and PV1 infection in vitro. The results provided a relevant possibility of developing novel approaches in the rational design of vaccines effective against both RV and PV.
authors.forename: X-X
authors.initials: XX
authors.lastname: Pan
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: W-Y
authors.initials: WY
authors.lastname: Xia
authors.forename: X-F
authors.initials: XF
authors.lastname: Li
authors.forename: L-J
authors.initials: LJ
authors.lastname: Yang
authors.forename: C
authors.initials: C
authors.lastname: Huang
authors.forename: Y-D
authors.initials: YD
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D006168:Guinea Pigs; D008958:Models, Molecular; D010957:Plasmids; D017955:Poliovirus / Q000145:classification*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000939:Epitopes; D011994:Recombinant Proteins; D014764:Viral Proteins
keywords: chimeric protein; neutralizing activity; rotavirus; poliovirus epitope.