title: An extracellular yellow laccase with potent dye decolorizing ability from the fungus Leucoagaricus naucinus LAC-04.
abstract: A novel laccase was isolated from fermentation broth of the mycorrhizal fungus Leucoagaricus naucinus LAC-04 by using a protocol that comprising ion-exchange chromatography steps on DEAE-cellulose, SP-Sepharose, and Q-Sepharose, and finally gel filtration by fast protein liquid chromatography on Superdex 75. The laccase (LNL) was purified with a purification fold of 21.19 and a recovery rate of 19.8%. It is a monomeric protein with a molecular mass of 56kDa. LNL lacks absorption around 600nm, which indicates that the purified laccase is a yellow laccases. LNL demonstrates an optimal pH of 2.2 and an optimal temperature range of 30-60°C using ABTS as the substrate. It is inhibited in the presence of EDTA and metal ions including Cd2+, Co2+, Cu2+. The Kmof the laccase towards ABTS is estimated to 50.12μM at pH 2.2 and 30°C. Moreover, the purified laccase manifests effective decolorizing activity towards azo, heterocyclic, and aromatic dyes including Bromothymol Blue, Eriochrome Black T, Evans Bue, Fuchsin Basic, and Remazol Brilliant Blue R.
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Ning
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Qing-Jun
authors.initials: QJ
authors.lastname: Chen
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Zhuo-Ren
authors.initials: ZR
authors.lastname: Ling
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Shou-Nan
authors.initials: SN
authors.lastname: Wang
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Wang
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China. Electronic address: zhanggq1001@gmail.com.
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Zhang
authors.affiliation: Department of Fungal resource, Shandong Agricultural University, Taian, Shandong 271018, China. Electronic address: mengfan7777@163.com.
authors.forename: Meng-Juan
authors.initials: MJ
authors.lastname: Zhu
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D004396:Coloring Agents / Q000737:chemistry*; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D004396:Coloring Agents; D005656:Fungal Proteins; D042845:Laccase
keywords: Dye decolorizing activity; Laccase; Leucoagaricus naucinus