title: Comparative Study of the Immunoregulatory Capacity of In Vitro Generated Tolerogenic Dendritic Cells, Suppressor Macrophages, and Myeloid-Derived Suppressor Cells.
abstract: Regulatory myeloid cell (RMC) therapy is a promising strategy for the treatment of immunological disorders such as autoimmune disease and allograft transplant rejection. Various RMC subsets can be derived from total bone marrow using different protocols, but their phenotypes often overlap, raising questions about whether they are truly distinct.
  
  
  
  In this study, we directly compared the phenotype and function of 3 types of RMCs, tolerogenic dendritic cells, suppressor macrophages, and myeloid-derived suppressor cells, generated in vitro from the same mouse strain in a single laboratory.
  
  
  
  We show that the 3 RMC subsets tested in this study share some phenotypic markers, suppress T cell proliferation in vitro and were all able to prolong allograft survival in a model of skin transplantation. However, our results highlight distinct mechanisms of action that are specific to each cell population.
  
  
  
  This study shows for the first time a side-by-side comparison of 3 types of RMCs using the same phenotypic and functional assays, thus providing a robust analysis of their similarities and differences.
authors.affiliation: 1 INSERM UMR1064, Center for Research in Transplantation and Immunology, Nantes, France.2 CHU Nantes, ITUN, Nantes, France.3 Nantes University, Faculté de Médecine, Nantes, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Carretero-Iglesia
authors.forename: Laurence
authors.initials: L
authors.lastname: Bouchet-Delbos
authors.forename: Cédric
authors.initials: C
authors.lastname: Louvet
authors.forename: Lucile
authors.initials: L
authors.lastname: Drujont
authors.forename: Mercedes
authors.initials: M
authors.lastname: Segovia
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Merieau
authors.forename: Elise
authors.initials: E
authors.lastname: Chiffoleau
authors.forename: Régis
authors.initials: R
authors.lastname: Josien
authors.forename: Marcelo
authors.initials: M
authors.lastname: Hill
authors.forename: Maria-Cristina
authors.initials: MC
authors.lastname: Cuturi
authors.forename: Aurélie
authors.initials: A
authors.lastname: Moreau
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D003713:Dendritic Cells / Q000502:physiology*; D006085:Graft Survival; D008213:Lymphocyte Activation; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D000072737:Myeloid-Derived Suppressor Cells / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology