title: Polysaccharides from Polygonatum Inhibit the Proliferation of Prostate Cancer-Associated Fibroblasts.
abstract: Inhibition of cancer-associated broblasts (CAFs) may improve the efficacy of cancer therapy. Polysaccharide extracted from polygonatum can selectively inhibit the growth of prostate-CAFs (<.001) without inhibiting the growth of normal broblasts (NAFs). Polysaccharides from polygonatum stimulate autophagy of prostate-CAFs. 3-methyl-adenine(3-MA) is an autophagy inhibitor. 3-MA was added to prostate-CAFs with polysaccharide from polygonatum to determine whether autophagy plays an important role in the restrained effect. Finally, polysaccharide from polygonatum treatment significantly increased the activation of Beclin-1 and LC3, key autophagy proteins. Polysaccharides from polygonatum stimulate autophagy of prostate-CAFs and inhibits prostate-CAF growth, indicating that a novel anti-cancer strategy involves inhibiting the growth of prostate- CAFs.
authors.affiliation: Institute of Biophysics, Chinese Academy of Science, Beijing, China E-mail : zhihai@ibp.ac.cn; tao@moon.ibp.ac.cn.
authors.forename: Shu-Yu
authors.initials: SY
authors.lastname: Han
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Hu
authors.forename: Guan-Yun
authors.initials: GY
authors.lastname: Qi
authors.forename: Chao-Xiong
authors.initials: CX
authors.lastname: Ma
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Wang
authors.forename: Fang-Li
authors.initials: FL
authors.lastname: Ma
authors.forename: Ning
authors.initials: N
authors.lastname: Tao
authors.forename: Zhi-Hai
authors.initials: ZH
authors.lastname: Qin
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy / Q000187:drug effects; D000072645:Cancer-Associated Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D031384:Polygonatum / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D011467:Prostate / Q000187:drug effects; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D008565:Membrane Proteins; D010936:Plant Extracts; D011134:Polysaccharides