title: An ultrasensitive electrochemiluminescence immunosensor for NT-proBNP based on self-catalyzed luminescence emitter coupled with PdCu@carbon nanohorn hybrid.
abstract: A novel electrochemiluminescence (ECL) signal amplified strategy based on self-catalyzed luminol derivative and carbon nanohorn-based hybrid for luminol/H2O2system was firstly proposed, and applied to fabricate an ultrasensitive ECL immunosensor for the detection of N-terminal pro-brain natriuretic peptides (NT-proBNP). Initially, PdCu nanocubes supported single-walled carbon nanohorns (PdCu@SWCNHs) hybrid was successfully synthesized through a facile one-pot strategy. For signal tag fabrication, 3,4,9,10-perylenetetracarboxylic acid (PTCA) conjugated luminol (PTC-Lu) as a novel self-catalyzed luminescence emitter was prepared and then effectively immobilized on the PdCu@SWCNHs to form PTC-Lu/PdCu@SWCNHs nanocomposite via π-π stacking. Herein, PTCA was selected and introduced into the luminol/H2O2ECL system for the first time, which could not only increase the luminescence signal but also remarkably improve the stability and hydrophilicity of pristine SWCNHs. More notably, PdCu@SWCNHs nanohybrid showed superior catalytic activity toward H2O2that could further amplify the ECL signal of luminol/H2O2system. Meanwhile, good biocompatibility and high specific surface area of PdCu nanocubes make the composite possess excellent property for immobilization of detection antibody. Based upon above, the proposed "signal on" immunosensor exhibited a wide linear detection range of 0.1pgmL-1to 25ngmL-1with a relatively low detection limit of 0.05pgmL-1, and successfully achieved the detection in clinical human serum samples with desirable results.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Chengyi
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yqchai@swu.edu.cn.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. Electronic address: yuanruo@swu.edu.cn.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002244:Carbon / Q000737:chemistry*; D002384:Catalysis; D003300:Copper / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D049449:Luminescence; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D020097:Natriuretic Peptide, Brain / Q000032:analysis / Q000097:blood*; D010165:Palladium / Q000737:chemistry*; D010446:Peptide Fragments / Q000032:analysis / Q000097:blood*; D010569:Perylene / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: C560550:3,4,9,10-perylene tetracarboxylic acid; D055910:Antibodies, Immobilized; D049408:Luminescent Agents; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain; D008165:Luminol; D010569:Perylene; D010165:Palladium; D002244:Carbon; D003300:Copper
keywords: Carbon nanohorn-based hybrid; Electrochemiluminescence; Immunosensor; N-terminal pro-brain natriuretic peptides; Self-catalyzed luminol derivative