title: Construction and co-expression of polycistronic plasmids encoding bio-degumming-related enzymes to improve the degumming process of ramie fibres.
abstract: OBJECTIVE
  To research the inherent properties of the co-expression of three types degumming-related enzymes and breed more powerful degumming strains.
  
  
  RESULTS
  Six tandem multimers of the pectate lyase gene, the xylanase gene, and the endo-1,4-β-mannanase gene, which are essential for degumming process, were co-expressed and evaluated in Escherichia coli BL21(DE3). The xyl91 gene had a synergistic effect with endo-1,4-β-mannanase and pectate lyase from DCE-01, when xyl gene was replaced with xyl91 in the multimer. The recombinant pET-pxm(91x) was selected and transformed into the original degumming strain DCE-01, which led to an enzymatic activity improvement. Furthermore, the weight loss, reducing sugar and COD value of the sample treated with the new engineered strain pET-pxm(91x)/DCE-01 increased to 22.5 %, 460 mg ml-1and 4.9, respectively.
  
  
  CONCLUSIONS
  The co-expression of degumming-related enzyme genes may be applied in industrial tests and represents a novel direction for bio-degumming research.
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Xianjiahu West road 348, Changsha, 410205, Hunan, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Xianjiahu West road 348, Changsha, 410205, Hunan, People's Republic of China. ibfclzc@189.cn.
authors.forename: Zhengchu
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Xianjiahu West road 348, Changsha, 410205, Hunan, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Xianjiahu West road 348, Changsha, 410205, Hunan, People's Republic of China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Xianjiahu West road 348, Changsha, 410205, Hunan, People's Republic of China.
authors.forename: Zhun
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Xianjiahu West road 348, Changsha, 410205, Hunan, People's Republic of China.
authors.forename: Shengwen
authors.initials: S
authors.lastname: Duan
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Xianjiahu West road 348, Changsha, 410205, Hunan, People's Republic of China.
authors.forename: Xiangyuan
authors.initials: X
authors.lastname: Feng
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Xianjiahu West road 348, Changsha, 410205, Hunan, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Xianjiahu West road 348, Changsha, 410205, Hunan, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Xianjiahu West road 348, Changsha, 410205, Hunan, People's Republic of China.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Wang
mesh_terms: D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D008361:Mannosidases / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011133:Polysaccharide-Lyases / Q000235:genetics*; D014995:Xylosidases / Q000235:genetics*
chemical_list: D008361:Mannosidases; D014995:Xylosidases; C041867:endo-1,4-beta-D-mannanase; D011133:Polysaccharide-Lyases; C023911:pectate lyase
keywords: BE-91; Bio-degumming; Co-expression; DCE-01; Ramie; Recombinant strains; xyl91