title: The temporally controlled expression of Drongo, the fruit fly homolog of AGFG1, is achieved in female germline cells via P-bodies and its localization requires functional Rab11.
abstract: To achieve proper RNA transport and localization, RNA viruses exploit cellular vesicular trafficking pathways. AGFG1, a host protein essential for HIV-1 and Influenza A replication, has been shown to mediate release of intron-containing viral RNAs from the perinuclear region. It is still unknown what its precise role in this release is, or whether AGFG1 also participates in cytoplasmic transport. We report for the first time the expression patterns during oogenesis for Drongo, the fruit fly homolog of AGFG1. We find that temporally controlled Drongo expression is achieved by translational repression of drongo mRNA within P-bodies. Here we show a first link between the recycling endosome pathway and Drongo, and find that proper Drongo localization at the oocyte's cortex during mid-oogenesis requires functional Rab11.
authors.affiliation: a Biological Sciences Department , Hunter College, City University of New York , New York , NY , USA.
authors.forename: Irina E
authors.initials: IE
authors.lastname: Catrina
authors.affiliation: a Biological Sciences Department , Hunter College, City University of New York , New York , NY , USA.|b Program in Molecular, Cellular, and Developmental Biology , The Graduate Center, City University of New York , New York , NY , USA.
authors.forename: Livia V
authors.initials: LV
authors.lastname: Bayer
authors.affiliation: a Biological Sciences Department , Hunter College, City University of New York , New York , NY , USA.
authors.forename: Giussepe
authors.initials: G
authors.lastname: Yanez
authors.affiliation: a Biological Sciences Department , Hunter College, City University of New York , New York , NY , USA.|b Program in Molecular, Cellular, and Developmental Biology , The Graduate Center, City University of New York , New York , NY , USA.
authors.forename: John M
authors.initials: JM
authors.lastname: McLaughlin
authors.affiliation: a Biological Sciences Department , Hunter College, City University of New York , New York , NY , USA.
authors.forename: Kornelia
authors.initials: K
authors.lastname: Malaczek
authors.affiliation: a Biological Sciences Department , Hunter College, City University of New York , New York , NY , USA.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Bagaeva
authors.affiliation: c Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute, New Jersey Medical School - Rutgers , The State University of New Jersey , Newark , NJ , USA.
authors.forename: Salvatore A E
authors.initials: SA
authors.lastname: Marras
authors.affiliation: a Biological Sciences Department , Hunter College, City University of New York , New York , NY , USA.|b Program in Molecular, Cellular, and Developmental Biology , The Graduate Center, City University of New York , New York , NY , USA.
authors.forename: Diana P
authors.initials: DP
authors.lastname: Bratu
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D009866:Oogenesis; D021381:Protein Transport; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D029721:Drosophila Proteins; D008840:Microfilament Proteins; D020691:rab GTP-Binding Proteins; C000618312:Drongo protein, Drosophila; C559123:Rab11 protein, Drosophila; D000099193:rab11 GTP-Binding Proteins
keywords: AGFG1; Drongo; Drosophila; HIV-1; Influenza A; Rab11; cytoskeleton