title: Alleviation of Drought Stress by Hydrogen Sulfide Is Partially Related to the Abscisic Acid Signaling Pathway in Wheat.
abstract: Little information is available describing the effects of exogenous H2S on the ABA pathway in the acquisition of drought tolerance in wheat. In this study, we investigated the physiological parameters, the transcription levels of several genes involved in the abscisic acid (ABA) metabolism pathway, and the ABA and H2S contents in wheat leaves and roots under drought stress in response to exogenous NaHS treatment. The results showed that pretreatment with NaHS significantly increased plant height and the leaf relative water content of seedlings under drought stress. Compared with drought stress treatment alone, H2S application increased antioxidant enzyme activities and reduced MDA and H2O2 contents in both leaves and roots. NaHS pretreatment increased the expression levels of ABA biosynthesis and ABA reactivation genes in leaves; whereas the expression levels of ABA biosynthesis and ABA catabolism genes were up-regulated in roots. These results indicated that ABA participates in drought tolerance induced by exogenous H2S, and that the responses in leaves and roots are different. The transcription levels of genes encoding ABA receptors were up-regulated in response to NaHS pretreatment under drought conditions in both leaves and roots. Correspondingly, the H2S contents in leaves and roots were increased by NaHS pretreatment, while the ABA contents of leaves and roots decreased. This implied that there is complex crosstalk between these two signal molecules, and that the alleviation of drought stress by H2S, at least in part, involves the ABA signaling pathway.
authors.affiliation: National Engineering Research Center for Wheat, Agronomy, Henan Agricultural University, Zhengzhou 450002, China.|The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Dongyun
authors.initials: D
authors.lastname: Ma
authors.affiliation: National Engineering Research Center for Wheat, Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Huina
authors.initials: H
authors.lastname: Ding
authors.affiliation: National Engineering Research Center for Wheat, Agronomy, Henan Agricultural University, Zhengzhou 450002, China.|The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Chenyang
authors.initials: C
authors.lastname: Wang
authors.affiliation: National Engineering Research Center for Wheat, Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Qin
authors.affiliation: National Engineering Research Center for Wheat, Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Qiaoxia
authors.initials: Q
authors.lastname: Han
authors.affiliation: National Engineering Research Center for Wheat, Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Hou
authors.affiliation: National Engineering Research Center for Wheat, Agronomy, Henan Agricultural University, Zhengzhou 450002, China.|The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 45002, China.
authors.forename: Hongfang
authors.initials: H
authors.lastname: Lu
authors.affiliation: National Engineering Research Center for Wheat, Agronomy, Henan Agricultural University, Zhengzhou 450002, China.|The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 45002, China.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Xie
authors.affiliation: National Engineering Research Center for Wheat, Agronomy, Henan Agricultural University, Zhengzhou 450002, China.|The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 45002, China.
authors.forename: Tiancai
authors.initials: T
authors.lastname: Guo
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism* / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000378:metabolism* / Q000494:pharmacology; D008315:Malondialdehyde / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013312:Stress, Physiological / Q000187:drug effects; D013440:Sulfides / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D014908:Triticum / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D013440:Sulfides; D008315:Malondialdehyde; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; C025451:sodium bisulfide; D006862:Hydrogen Sulfide