title: [Etiology and drug resistance of non-typhiSalmonellain Henan province, 2011-2015].
abstract: Objective:To analyze the serotypes and antibiotic resistance phenotypes of non-typhiSalmonellastrains in Henan province from 2011 to 2015.Methods:The stool samples were collected from diarrhea patients, and enriched with SBG enrichment broth and the pathogen isolation was conducted with CHROMAgar selective culture medium at 37 ℃ for 18-24 hours using KIA/MIU biochemical action and API20E biochemical system slab to identifySalmonellastrains. The serotypes of all the positive strains were detected with SSISalmonellatyping sera. According to K-B drug susceptibility testing method published by USA clinical and Laboratory Standards Institute (CLSI), the antibiotics resistant phenotype of the positive strains were analyzed.Results:A total of 1 351 strains of non-typhiSalmonellawere isolated, in which 811 were from males, 540 were from females. The ratio of men to women was 1.5∶1. Children and young adults were mainly affected. The pathogen isolation was mainly in May-October during a year. The 1 351 strains of non-typhiSalmonllawere divided into 58 serotypes.S. enteritidis, S. typhimurium, S. agona, S. derby, S. indiana, S. senftenbergandS. thompsonranked 1st-7th. The drug-resistance rate of the 1 351 strains was 46.1% to synthetic broad-spectrum penicillin ampicillin (AMP), 19.5% and 21.2% to the three generation cephalosporin ceftazidime (CAZ) and cefotaxime (CTX), 8.8% to the four generation cephalosporins cefepime (FEP), 58.7% to the one generation of quinolones nalidixic acid (NAL), 14.7% to the three generation fluoroquinolone ciprofloxacin (CIP) and norfloxacin (NOR), 25.0% and 35.6% to aminoglycoside antibiotic gentamicin (GEN) and streptomycin (STR), 35.4% to amphenicols (CHL), 31.7% to sulfonamide trimethoprim (TMP) and 37.8% to tetracycline (TET). Totally 879 strains were multidrug resistant (65.1%): 350 strains were resistant to 3-4 kinds of antibiotics (25.9%), 309 strains were resistant to 5-7 kinds of antibiotics (22.9%), 174 strains were resistant to 8-10 kinds of antibiotics (12.9%) and 48 strains were resistant to 11-12 kinds of antibiotics (3.6%).Conclusion:The serotypes of non-typhiSalmonellastrains isolated from Henan province varied, some strains were resistant to 12 kinds of antibiotics commonly used in clinical treatment and the multidrug resistance has become serious.
authors.affiliation: Pathogenic Microbiology Laboratory of Institute for Infectious Disease Control and Prevention, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhao
authors.forename: Y J
authors.initials: YJ
authors.lastname: Mu
authors.forename: B F
authors.initials: BF
authors.lastname: Zhang
authors.forename: S L
authors.initials: SL
authors.lastname: Xia
authors.forename: J
authors.initials: J
authors.lastname: Su
authors.forename: X Y
authors.initials: XY
authors.lastname: Huang
authors.forename: B L
authors.initials: BL
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents; D000077723:Cefepime; D002511:Cephalosporins; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000188:drug therapy / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D012475:Salmonella; D013307:Streptomycin; D013752:Tetracycline; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D000077723:Cefepime; D013752:Tetracycline; D013307:Streptomycin