title: A potential inhibitory function of draxin in regulating mouse trunk neural crest migration.
abstract: Draxin is a repulsive axon guidance protein that plays important roles in the formation of three commissures in the central nervous system and dorsal interneuron 3 (dI3) in the chick spinal cord. In the present study, we report the expression pattern of mouse draxin in the embryonic mouse trunk spinal cord. In the presence of draxin, the longest net migration length of a migrating mouse trunk neural crest cell was significantly reduced. In addition, the relative number of apolar neural crest cells increased as the draxin treatment time increased. Draxin caused actin cytoskeleton rearrangement in the migrating trunk neural crest cells. Our data suggest that draxin may regulate mouse trunk neural crest cell migration by the rearrangement of cell actin cytoskeleton and by reducing the polarization activity of these cells subsequently.
authors.affiliation: Department of Hand and Foot Surgery, The Third Hospital of Shijiazhuang City, 15 South Tiyu Street, Shijiazhuang, 050000, People's Republic of China. zhangsanfeng80@hotmail.com.
authors.forename: Sanbing
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Human Anatomy, Hebei Medical University, No. 361 East Zhongshan Road, Shijiazhuang, Hebei Province, 050017, People's Republic of China. suyuh1227@hotmail.com.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Hand and Foot Surgery, The Third Hospital of Shijiazhuang City, 15 South Tiyu Street, Shijiazhuang, 050000, People's Republic of China.
authors.forename: Jinbao
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Hand and Foot Surgery, The Third Hospital of Shijiazhuang City, 15 South Tiyu Street, Shijiazhuang, 050000, People's Republic of China.
authors.forename: Chenbing
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Division of Developmental Neurobiology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D019556:COS Cells; D002465:Cell Movement; D016764:Cell Polarity / Q000187:drug effects; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D018507:Gene Expression Regulation, Developmental; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D018345:Mice, Knockout; D009432:Neural Crest / Q000166:cytology* / Q000378:metabolism*; D054259:Neural Tube / Q000166:cytology; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D013116:Spinal Cord / Q000378:metabolism; D060726:Torso
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D011993:Recombinant Fusion Proteins; C534831:draxin protein, mouse
keywords: Actin cytoskeleton; Axon guidance; Draxin; Migration; Spinal cord; Trunk neural crest