title: The Suppressive Effects of Cinnamomi Cortex and Its Phytocompound Coumarin on Oxaliplatin-Induced Neuropathic Cold Allodynia in Rats.
abstract: Oxaliplatin, a chemotherapy drug, induces acute peripheral neuropathy characterized by cold allodynia, spinal glial activation and increased levels of pro-inflammatory cytokines. Herein, we determined whether Cinnamomi Cortex (C. Cortex), a widely used medicinal herb in East Asia for cold-related diseases, could attenuate oxaliplatin-induced cold allodynia in rats and the mechanisms involved. A single oxaliplatin injection (6 mg/kg, i.p.) induced significant cold allodynia signs based on tail immersion tests using cold water (4 °C). Daily oral administration of water extract of C. Cortex (WECC) (100, 200, and 400 mg/kg) for five consecutive days following an oxaliplatin injection dose-dependently alleviated cold allodynia with only a slight difference in efficacies between the middle dose at 200 mg/kg and the highest dose at 400 mg/kg. WECC at 200 mg/kg significantly suppressed the activation of astrocytes and microglia and decreased the expression levels of IL-1β and TNF in the spinal cord after injection with oxaliplatin. Furthermore, oral administration of coumarin (10 mg/kg), a major phytocompound of C. Cortex, markedly reduced cold allodynia. These results indicate that C. Cortex has a potent anti-allodynic effect in oxaliplatin-injected rats through inhibiting spinal glial cells and pro-inflammatory cytokines. We also suggest that coumarin might play a role in the anti-allodynic effect of C. Cortex.
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, 26 Kyunghee-daero, Dongdamoon-gu, Seoul 02447, Korea. ckdals4302@naver.com.
authors.forename: Changmin
authors.initials: C
authors.lastname: Kim
authors.affiliation: Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 26 Kyunghee-daero, Dongdamoon-gu, Seoul 02447, Korea. mibdna@gmail.com.
authors.forename: Ji Hwan
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, 26 Kyunghee-daero, Dongdamoon-gu, Seoul 02447, Korea. thasnow@gmail.com.
authors.forename: Woojin
authors.initials: W
authors.lastname: Kim
authors.affiliation: Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 573 Xujiahui Rd., Dapiqiao, Huangpu Qu, Shanghai 200025, China. leedongxing@naver.com.
authors.forename: Dongxing
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, 26 Kyunghee-daero, Dongdamoon-gu, Seoul 02447, Korea. yskim1158@khu.ac.kr.
authors.forename: Yangseok
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, 26 Kyunghee-daero, Dongdamoon-gu, Seoul 02447, Korea. niceday@khu.ac.kr.
authors.forename: Kyungjin
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, 26 Kyunghee-daero, Dongdamoon-gu, Seoul 02447, Korea. skkim77@khu.ac.kr.|Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 26 Kyunghee-daero, Dongdamoon-gu, Seoul 02447, Korea. skkim77@khu.ac.kr.
authors.forename: Sun Kwang
authors.initials: SK
authors.lastname: Kim
keywords: Cinnamomi Cortex; cold allodynia; coumarin; glia; pro-inflammatory cytokines; spinal cord