title: Protective effects of TES trioleate, an inhibitor of phospholipase A2, on reactive oxygen species and UVA-induced cell damage.
abstract: 2-[Tris(oleoyloxymethyl)methylamino]-1-ethane sulfonic acid (TES trioleate) is an inhibitor of phospholipase A2(PLA2), which hydrolyzes cell membrane phospholipids to produce arachidonic acid (AA) and lysophospholipids (LysoPLs). Here, we investigated the protective effects of TES trioleate on cell damage caused by ultraviolet A (UVA) light and reactive oxygen species (ROS). Pre-incubation with 250-1000μM TES trioleate resulted in concentration-dependent protection from UVA-induced damage in HaCaT cells. Additionally, 25-1000μM TES trioleate provided protection against H2O2in a concentration-dependent manner. In human erythrocytes treated with1O2, 10-100μM TES trioleate showed concentration-dependent protective effects, similar to but stronger than the controls, 4-BPB and lipophilic antioxidant (+)-α-tocopherol at 100μM. TES trioleate did not have detectable radical scavenging activity. Moreover, compared with (+)-α-tocopherol and rutin, TES trioleate showed low ROS scavenging activity. Thus, although TES trioleate showed cell protective effects against UVA, H2O2, and1O2-induced damages, these effects were not caused by the scavenging ability of the radical or ROS. Finally, pretreatment of HaCaT cells and human erythrocytes with l-α-lysophosphatidylcholine produced by PLA2 promoted increased cell damage at low concentrations. Thus, the protective effects of TES trioleate on cellular damage by UVA and ROS may be associated with inhibition of PLA2-dependent cell damage rather than ROS scavenging.
authors.affiliation: Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Republic of Korea. Electronic address: snpark@seoultech.ac.kr.
authors.forename: Soo Nam
authors.initials: SN
authors.lastname: Park
authors.affiliation: Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Republic of Korea.
authors.forename: Moon Jin
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Republic of Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Ha
authors.affiliation: Department of Fine Chemistry, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Republic of Korea.
authors.forename: Nan Hee
authors.initials: NH
authors.lastname: Lee
authors.affiliation: Daebong LS. Ltd., 692-8, Gojan-dong, Namdong-gu, Incheon 21697, Republic of Korea.
authors.forename: Jino
authors.initials: J
authors.lastname: Park
authors.affiliation: UCL Co., 692-9, Gojan-dong, Namdong-gu, Incheon 21697, Republic of Korea.
authors.forename: Jiwon
authors.initials: J
authors.lastname: Lee
authors.affiliation: WYNN Clinic, 1317-11, Seocho-dong, Seocho-gu, Seoul 06615, Republic of Korea.
authors.forename: Dukha
authors.initials: D
authors.lastname: Kim
authors.affiliation: WYNN Clinic, 1317-11, Seocho-dong, Seocho-gu, Seoul 06615, Republic of Korea.
authors.forename: Chulsoo
authors.initials: C
authors.lastname: Yoon
mesh_terms: D002460:Cell Line; D006801:Humans; D064801:Phospholipase A2 Inhibitors / Q000494:pharmacology*; D054467:Phospholipases A2 / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014325:Tromethamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D064801:Phospholipase A2 Inhibitors; D017382:Reactive Oxygen Species; D014325:Tromethamine; C004551:TES; D054467:Phospholipases A2
keywords: 2-[Tris(oleoyloxymethyl)methylamino]-1-ethane sulfonic acid; Phospholipase A(2) inhibitor; Protective effects; Reactive oxygen species; Ultraviolet A