title: Comparative analysis of gene expression profiles of OPN signaling pathway in four kinds of liver diseases.
abstract: To explore the relevance of OPN signalling pathway to the occurrence and development of nonalcoholic fatty liver disease (NAFLD), liver cirrhosis (LC), hepatic cancer (HC) and acute hepatic failure (AHF) at transcriptional level, Rat Genome 230 2.0 Array was used to detect expression profiles of OPN signalling pathway-related genes in four kinds of liver diseases. The results showed that 23, 33, 59 and 74 genes were significantly changed in the above four kinds of liver diseases, respectively. H-clustering analysis showed that the expression profiles of OPN signalling-related genes were notably different in four kinds of liver diseases. Subsequently, a total of above-mentioned 147 genes were categorized into four clusters by k-means according to the similarity of gene expression, and expression analysis systematic explorer (EASE) functional enrichment analysis revealed that OPN signalling pathway-related genes were involved in cell adhesion and migration, cell proliferation, apoptosis, stress and inflammatory reaction, etc. Finally, ingenuity pathway analysis (IPA) software was used to predict the functions of OPN signalling-related genes, and the results indicated that the activities of ROS production, cell adhesion and migration, cell proliferation were remarkably increased, while that of apoptosis, stress and inflammatory reaction were reduced in four kinds of liver diseases. In summary, the above physiological activities changed more obviously in LC, HC and AHF than in NAFLD.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang 453007, Henan, People's Republic of China.cellkeylab@126.com.
authors.forename: Gaiping
authors.initials: G
authors.lastname: Wang
authors.forename: Shasha
authors.initials: S
authors.lastname: Chen
authors.forename: Congcong
authors.initials: C
authors.lastname: Zhao
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Li
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Cuifang
authors.initials: C
authors.lastname: Chang
authors.forename: Cunshuan
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D019295:Computational Biology; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D055106:Genome-Wide Association Study; D008103:Liver Cirrhosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D017114:Liver Failure, Acute / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D053858:Metabolic Networks and Pathways; D065626:Non-alcoholic Fatty Liver Disease / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D053495:Osteopontin / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: C506897:Spp1 protein, rat; D053495:Osteopontin