title: The Use of Signal-Transduction and Metabolic Pathways to Predict Human Disease Targets from Electric and Magnetic Fields Using in vitro Data in Human Cell Lines.
abstract: Using in vitro data in human cell lines, several research groups have investigated changes in gene expression in cellular systems following exposure to extremely low frequency (ELF) and radiofrequency (RF) electromagnetic fields (EMF). For ELF EMF, we obtained five studies with complete microarray data and three studies with only lists of significantly altered genes. Likewise, for RF EMF, we obtained 13 complete microarray datasets and 5 limited datasets. Plausible linkages between exposure to ELF and RF EMF and human diseases were identified using a three-step process: (a) linking genes associated with classes of human diseases to molecular pathways, (b) linking pathways to ELF and RF EMF microarray data, and (c) identifying associations between human disease and EMF exposures where the pathways are significantly similar. A total of 60 pathways were associated with human diseases, mostly focused on basic cellular functions like JAK-STAT signaling or metabolic functions like xenobiotic metabolism by cytochrome P450 enzymes. ELF EMF datasets were sporadically linked to human diseases, but no clear pattern emerged. Individual datasets showed some linkage to cancer, chemical dependency, metabolic disorders, and neurological disorders. RF EMF datasets were not strongly linked to any disorders but strongly linked to changes in several pathways. Based on these analyses, the most promising area for further research would be to focus on EMF and neurological function and disorders.
authors.affiliation: National Institute of Environmental Health Sciences, Research Triangle Park , Durham, NC , USA.
authors.forename: Fred
authors.initials: F
authors.lastname: Parham
authors.affiliation: Environmental Health Research , Thun , Switzerland.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Portier
authors.affiliation: National Institute of Environmental Health Sciences, Research Triangle Park , Durham, NC , USA.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chang
authors.affiliation: Division of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty , University of Bern, Bern , Switzerland.
authors.forename: Meike
authors.initials: M
authors.lastname: Mevissen
keywords: electromagnetic fields; gene set analysis; genomics; human disease; metabolic pathways; microarray gene expression; signal-transduction pathways