title: Membrane Rigidity Determined by Atomic Force Microscopy Is a Parameter of the Permeability of Liposomal Membranes to the Hydrophilic Compound Calcein.
abstract: We determined the permeability coefficient of a model hydrophilic drug, calcein, encapsulated within saturated lipid-based nano-sized liposomes of various lipid profiles. We demonstrated that the addition of cholesterol to liposomes containing saturated lipids increased the permeability of the liposomal membrane to calcein via a decrease in the membrane bending modulus, as determined by means of atomic force microscopy. We found an inverse correlation between the membrane bending modulus of saturated lipid-based nano-sized liposomes and the permeability coefficient of encapsulated calcein, demonstrating that bending modulus, as determined by means of atomic force microscopy, is a quantitative parameter describing the permeability of liposomal membranes to calcein.
authors.affiliation: Japan Agency for Medical Research and Development, 1-7-1 Otemachi, Chiyoda-ku, Tokyo, 100-0004, Japan.|Division of Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takechi-Haraya
authors.affiliation: Division of Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan. kumikato@nihs.go.jp.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sakai-Kato
authors.affiliation: Division of Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Goda
mesh_terms: D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism; D005452:Fluoresceins / Q000737:chemistry* / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes; D018625:Microscopy, Atomic Force / Q000379:methods*; D010539:Permeability
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D008081:Liposomes; D002784:Cholesterol; C007740:fluorexon
keywords: atomic force microscopy; calcein; liposomal membrane permeability; liposome; membrane rigidity