title: Re-implantation of cryopreserved ovarian cortex resulting in restoration of ovarian function, natural conception and successful pregnancy after haematopoietic stem cell transplantation for Wilms tumour.
abstract: With the improvement of long-term cancer survival rates, growing numbers of female survivors are suffering from treatment-related premature ovarian insufficiency (POI). Although pre-treatment embryo and oocyte storage are effective fertility preservation strategies, they are not possible for pre-pubertal girls or women who cannot delay treatment. In these cases, the only available treatment option is ovarian cortex cryopreservation and subsequent re-implantation. A 32-year-old woman had ovarian cortex cryopreserved 10 years previously before commencing high-dose chemotherapy and undergoing a haematopoietic stem cell transplant for recurrent adult Wilms tumour, which resulted in POI. She underwent laparoscopic orthotopic transplantation of cryopreserved ovarian cortex to the original site of biopsy on the left ovary. She ovulated at 15 and 29 weeks post-re-implantation with AMH detectable, then rising, from 21 weeks, and conceived naturally following the second ovulation. The pregnancy was uncomplicated and a healthy male infant was born by elective Caesarean section at 36+4weeks gestation. This is the first report of ovarian cortex re-implantation in the UK. Despite the patient receiving low-risk chemotherapy prior to cryopreservation and the prolonged tissue storage duration, the re-implantation resulted in rapid restoration of ovarian function and natural conception with successful pregnancy.
authors.affiliation: Simpson's Centre for Reproductive Health, Royal Infirmary of Edinburgh, Edinburgh, EH16 4SA, UK. Cheryl.Dunlop@ed.ac.uk.|MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, EH16 4TJ, UK. Cheryl.Dunlop@ed.ac.uk.
authors.forename: C E
authors.initials: CE
authors.lastname: Dunlop
authors.affiliation: Simpson's Centre for Reproductive Health, Royal Infirmary of Edinburgh, Edinburgh, EH16 4SA, UK.
authors.forename: B M
authors.initials: BM
authors.lastname: Brady
authors.affiliation: Centre for Integrative Physiology, University of Edinburgh, Edinburgh, EH8 9XD, UK.
authors.forename: M
authors.initials: M
authors.lastname: McLaughlin
authors.affiliation: Centre for Integrative Physiology, University of Edinburgh, Edinburgh, EH8 9XD, UK.
authors.forename: E E
authors.initials: EE
authors.lastname: Telfer
authors.affiliation: Beatson West of Scotland Cancer Centre, Glasgow, G12 0YN, UK.
authors.forename: J
authors.initials: J
authors.lastname: White
authors.affiliation: Beatson West of Scotland Cancer Centre, Glasgow, G12 0YN, UK.
authors.forename: F
authors.initials: F
authors.lastname: Cowie
authors.affiliation: Tissue and Cells Services, Scottish National Blood Transfusion Service, Edinburgh, EH17 7QT, UK.
authors.forename: S
authors.initials: S
authors.lastname: Zahra
authors.affiliation: Royal Hospital for Sick Children, Edinburgh, EH9 1LF, UK.
authors.forename: W H B
authors.initials: WH
authors.lastname: Wallace
authors.affiliation: Simpson's Centre for Reproductive Health, Royal Infirmary of Edinburgh, Edinburgh, EH16 4SA, UK.|MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, EH16 4TJ, UK.
authors.forename: R A
authors.initials: RA
authors.lastname: Anderson
mesh_terms: D000328:Adult; D015925:Cryopreservation; D005260:Female; D059247:Fertility Preservation; D005718:Gametogenesis / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D050498:Live Birth; D009364:Neoplasm Recurrence, Local / Q000473:pathology / Q000628:therapy; D009865:Oocytes / Q000254:growth & development / Q000473:pathology; D010053:Ovary / Q000254:growth & development / Q000473:pathology; D011247:Pregnancy; D011252:Pregnancy Complications, Neoplastic; D006113:United Kingdom; D009396:Wilms Tumor / Q000150:complications / Q000473:pathology / Q000628:therapy*
keywords: AMH; Fertility preservation; Ovarian cortex re-implantation; Premature ovarian insufficiency