title: Enterovirus 71 inhibits cellular type I interferon signaling by inhibiting host RIG-I ubiquitination.
abstract: Enterovirus 71 (EV71) is a human pathogen that induces hand, foot, and mouth disease (HFMD) and fatal neurological diseases in young children and infants. Pathogenicity of EV71 is likely related to its ability to evade host innate immunity through inhibiting cellular type I interferon signaling. However, it is less well understood the molecular events governing this process. In this study, we found that EV71 infection suppressed the induction of antiviral immunity by inhibiting the expression levels of IFN-β and IFN-stimulated genes (ISGs), such as ISG54 and ISG56, at the late stage of viral infection. At the same time, our results showed that EV71 infection significantly inhibited ubiquitination of RIG-I. In contrast, up-regulation of RIG-I ubiquitination promoted expression of IFN-β and ISGs, suggesting that inhibition of cellular type I interferon signaling was caused by down-regulation of RIG-I ubiquitination during EV71 infection. These results suggest that inhibition of RIG-I-mediated type I IFN responses by EV71 may contribute to the pathogenesis of viral infection.
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
authors.forename: Xingzhi
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Nephrology, The Fifth Affiliated Hospital of Harbin Medical University, Daqing, 163319, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
authors.forename: Ziye
authors.initials: Z
authors.lastname: Pan
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ding
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
authors.forename: Dehua
authors.initials: D
authors.lastname: Zou
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zheng
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
authors.forename: Liyang
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Xiao
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
authors.forename: Baifen
authors.initials: B
authors.lastname: Song
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China. Electronic address: cuiyudong@yahoo.com.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Cui
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China. Electronic address: 549076831@qq.com.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Cao
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China. Electronic address: 673365630@qq.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D000071457:DEAD Box Protein 58 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D029821:Enterovirus A, Human / Q000472:pathogenicity / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D057131:Immune Evasion; D007370:Interferon Type I / Q000037:antagonists & inhibitors*; D011499:Protein Processing, Post-Translational; D011971:Receptors, Immunologic; D015398:Signal Transduction; D054875:Ubiquitination
chemical_list: D007370:Interferon Type I; D011971:Receptors, Immunologic; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58
keywords: Enterovirus 71; RIG-I; Type I interferon; Ubiquitination