title: Inhibitory effects of tributyl phosphate on algal growth, photosynthesis, and fatty acid synthesis in the marine diatom Phaeodactylum tricornutum.
abstract: The widely used solvent extractant, tributyl phosphate (TBP), primarily used as a solvent for the conventional processing of nuclear fuel, has come under scrutiny recently due to concerns surrounding potential environmental contamination and toxicity. In this study, we found that, in Phaeodactylum tricornutum, administration of TBP severely inhibited algal cell growth by reducing photosynthetic efficiency and inducing oxidative stress. We further explored the effect of TBP by examining the gene expression of the photosynthetic electron transport chain and its contribution to reactive oxygen species (ROS) burst. Our data revealed that TBP affected both fatty acid content and profile by regulating the transcription of genes related to glycolysis, fatty acid biosynthesis, and β-oxidation. These results demonstrated that TBP did in fact trigger the synthesis of ROS, disrupting the subcellular membrane structure of this aquatic organism. Our study brings new insight into the fundamental mechanism of toxicity exerted by TBP on the marine alga P. tricornutum.
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Song
authors.affiliation: College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.
authors.forename: Xiaoji
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.
authors.forename: Guangfu
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Tan
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China. hfqian@zjut.edu.cn.|Xinjiang Key Laboratory of Environmental Pollution and Bioremediation, Chinese Academy of Sciences, Urumqi, 830011, People's Republic of China. hfqian@zjut.edu.cn.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Qian
mesh_terms: D017377:Diatoms / Q000187:drug effects* / Q000254:growth & development; D005227:Fatty Acids / Q000494:pharmacology*; D010755:Organophosphates / Q000494:pharmacology*; D010788:Photosynthesis; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D010755:Organophosphates; D017382:Reactive Oxygen Species; C009524:tributyl phosphate
keywords: Electron transfer chains; Fatty acid; Gene transcript; Phaeodactylum tricornutum; Tributyl phosphate