title: Perilla Oil Supplementation Ameliorates High-Fat/High-Cholesterol Diet Induced Nonalcoholic Fatty Liver Disease in Rats via Enhanced Fecal Cholesterol and Bile Acid Excretion.
abstract: Recent experimental studies and clinical trials have shown that hepatic cholesterol metabolic disorders are closely related to the development of nonalcoholic fatty liver disease (NAFLD). The main goal of this study was to investigate the efficacy of the perilla oil rich in alpha-linolenic acid (ALA) against NASH and gain a deep insight into its potential mechanisms. Rats were fed a high-fat/high-cholesterol diet (HFD) supplement with perilla oil (POH) for 16 weeks. Routine blood biochemical tests and histological staining illustrated that the perilla oil administration improved HFD-induced hyperlipidemia, reduced hepatic steatosis, and inhibited hepatic inflammatory infiltration and fibrosis. Perilla oil also increased fecal bile acid and cholesterol excretion. Hepatic RNA-Seq analysis found that the long time perilla oil supplement notably modified the gene expression involved in cholesterol metabolism. Our results implicate that, after long-term high level dietary cholesterol feeding, rat liver endogenous synthesis of cholesterol and cholesterol-rich low density lipoprotein uptake was significantly inhibited, and perilla oil did not modulate expression of genes responsible for cholesterol synthesis but did increase cholesterol removed from hepatocytes by conversion to bile acids and increased fecal cholesterol excretion.
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Ting
authors.identifier: 0000-0002-1275-0963
authors.initials: T
authors.lastname: Chen
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China; School of Medicine, Jianghan University, Wuhan, Hubei, China.
authors.forename: Fahu
authors.identifier: 0000-0001-7601-3797
authors.initials: F
authors.lastname: Yuan
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Hualin
authors.identifier: 0000-0002-8129-077X
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Blood Transfusion, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Lei
authors.initials: L
authors.lastname: He
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shao
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, China.
authors.forename: Zhiguo
authors.identifier: 0000-0001-7325-6478
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D059305:Diet, High-Fat; D019587:Dietary Supplements; D005243:Feces; D015723:Gene Library; D007249:Inflammation; D008055:Lipids / Q000737:chemistry; D008099:Liver / Q000378:metabolism; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017962:alpha-Linolenic Acid / Q000737:chemistry*
chemical_list: D001647:Bile Acids and Salts; D002791:Cholesterol, Dietary; D008055:Lipids; D010938:Plant Oils; D012333:RNA, Messenger; D017962:alpha-Linolenic Acid; C058216:perilla seed oil; D002784:Cholesterol