title: ATPase and GTPase Tangos Drive Intracellular Protein Transport.
abstract: The GTPase superfamily of proteins provides molecular switches to regulate numerous cellular processes. The 'GTPase switch' paradigm, in which external regulatory factors control the switch of a GTPase between 'on' and 'off' states, has been used to interpret the regulatory mechanism of many GTPases. However, recent work unveiled a class of nucleotide hydrolases that do not adhere to this classical paradigm. Instead, they use nucleotide-dependent dimerization cycles to regulate key cellular processes. In this review article, recent studies of dimeric GTPases and ATPases involved in intracellular protein targeting are summarized. It is suggested that these proteins can use the conformational plasticity at their dimer interface to generate multiple points of regulation, thereby providing the driving force and spatiotemporal coordination of complex cellular pathways.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA. Electronic address: sshan@caltech.edu.
authors.forename: Shu-Ou
authors.initials: SO
authors.lastname: Shan
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D020558:GTP Phosphohydrolases / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D020662:Guanine Nucleotide Exchange Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009708:Nucleotidases / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D000072756:Protein Conformation, alpha-Helical; D000072757:Protein Conformation, beta-Strand; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D020662:Guanine Nucleotide Exchange Factors; D029701:Saccharomyces cerevisiae Proteins; D009708:Nucleotidases; C030287:nucleotidase; D000251:Adenosine Triphosphatases; D020558:GTP Phosphohydrolases; C529048:Get3 protein, S cerevisiae
keywords: ATPases; GTPases; Get3; molecular recognition and regulation; protein targeting; signal recognition particle