title: Sorting nexin 9 (SNX9) is not essential for development and auditory function in mice.
abstract: Sorting nexins are a large family of evolutionarily conserved proteins that play fundamental roles in endocytosis, endosomal sorting and signaling. As an important member of sorting nexin family, sorting nexin 9 (SNX9) has been shown to participate in coordinating actin polymerization with membrane tubulation and vesicle formation. We previously showed that SNX9 is expressed in mouse auditory hair cells and might regulate actin polymerization in those cells. To further examine the physiological role of SNX9, we generated Snx9 knockout mice using homologous recombination method. Unexpectedly, Snx9 knockout mice have normal viability and fertility, and are morphologically and behaviorally indistinguishable from control mice. Further investigation revealed that the morphology and function of auditory hair cells are not affected by Snx9 inactivation, and Snx9 knockout mice have normal hearing threshold. In conclusion, our data revealed that Snx9-deficient mice do not show defects in development as well as auditory function, suggesting that SNX9 is not essential for mice development and hearing.
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, P. R. China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, P. R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhai
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, P. R. China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Du
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, P. R. China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, P. R. China.
authors.forename: Huiren
authors.initials: H
authors.lastname: Cao
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, P. R. China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Physiology, Shandong University School of Medicine, Jinan, Shandong 250012, P. R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yu
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, P. R. China.
authors.forename: Jiangang
authors.initials: J
authors.lastname: Gao
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, P. R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000199:Actins / Q000737:chemistry; D000818:Animals; D001522:Behavior, Animal; D004705:Endocytosis; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006198:Hair Cells, Auditory / Q000502:physiology*; D006309:Hearing / Q000502:physiology*; D059765:Homologous Recombination; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009622:Noise; D012333:RNA, Messenger / Q000378:metabolism; D058305:Sorting Nexins / Q000235:genetics / Q000502:physiology*
chemical_list: D000199:Actins; D012333:RNA, Messenger; C000626585:SNX9 protein, mouse; D058305:Sorting Nexins
keywords: SNX9; hair cells; hearing; inner ear; knockout mice