title: A novel method to determine valnemulin in feedingstuffs for several animal species by liquid chromatography-electrospray tandem mass spectrometry.
abstract: A new method specific for the determination and unambiguous confirmation of the antibiotic valnemulin in feed for all animal species is described. Simple clean-up based on solvent extraction and liquid partition is carried out prior to determination by liquid chromatography coupled to electrospray tandem mass spectrometry on a hybrid QTRAP 4000 system, in multiple reaction monitoring mode. Valnemulin is licensed in the European Union as a veterinary drug for use in feed for pigs and rabbits, but unavoidable carryover in feed for non-target species should be monitored. The method is rapid and reliable, and was validated in-house at 10, 100 and 750 ng g-1, evaluating the analytical performances according to Commission Regulation (EC) No. 882/2004. Good mean recoveries from 79.7% to 92.6%, and within-laboratory reproducibility RSDs, ranging from 6.2% to 12.1%, were measured; the limit of quantification at 10.0 ng g-1also allows for sensitive evaluation of undesirable carryover in feed for non-target species. The results of monitoring 24 compound feeds for several animal species are also reported.
authors.affiliation: a Dipartimento di Chimica , Istituto Zooprofilattico Sperimentale del Mezzogiorno , Portici , Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Gallo
authors.affiliation: a Dipartimento di Chimica , Istituto Zooprofilattico Sperimentale del Mezzogiorno , Portici , Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Di Marco Pisciottano
authors.affiliation: a Dipartimento di Chimica , Istituto Zooprofilattico Sperimentale del Mezzogiorno , Portici , Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Urbani
authors.affiliation: a Dipartimento di Chimica , Istituto Zooprofilattico Sperimentale del Mezzogiorno , Portici , Italy.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Bianco
authors.affiliation: a Dipartimento di Chimica , Istituto Zooprofilattico Sperimentale del Mezzogiorno , Portici , Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Picarelli
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes / Q000032:analysis; D006041:Goats; D011200:Poultry; D011817:Rabbits; D012756:Sheep; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004224:Diterpenes; C117845:valnemulin
keywords: Valnemulin; antibiotic carryover; feed; mass spectrometry