title: The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products.
abstract: Reliable methods are needed to detect the presence of tobacco components in tobacco products to effectively control smuggling and classify tariff and excise in tobacco industry to control illegal tobacco trade. In this study, two sensitive and specific DNA based methods, one quantitative real-time PCR (qPCR) assay and the other loop-mediated isothermal amplification (LAMP) assay, were developed for the reliable and efficient detection of the presence of tobacco (Nicotiana tabacum) in various tobacco samples and commodities. Both assays targeted the same sequence of the uridine 5'-monophosphate synthase (UMPS), and their specificities and sensitivities were determined with various plant materials. Both qPCR and LAMP methods were reliable and accurate in the rapid detection of tobacco components in various practical samples, including customs samples, reconstituted tobacco samples, and locally purchased cigarettes, showing high potential for their application in tobacco identification, particularly in the special cases where the morphology or chemical compositions of tobacco have been disrupted. Therefore, combining both methods would facilitate not only the detection of tobacco smuggling control, but also the detection of tariff classification and of excise.
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.
authors.forename: Sukumar
authors.identifier: 0000-0002-2899-2991
authors.initials: S
authors.lastname: Biswas
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fan
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.
authors.forename: Sifan
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Tobacco Research, Henan Academy of Agricultural Sciences, Xuchang, Henan 461000, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Ping
authors.affiliation: Institute of Tobacco Research, Henan Academy of Agricultural Sciences, Xuchang, Henan 461000, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Li
authors.affiliation: Central Customs Laboratory, Bulgarian Customs Agency, G. S. Rakovski Street 47, 1202 Sofia, Bulgaria.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Naumova
authors.affiliation: Dutch Customs Laboratory, Kingsfordweg 1, 1043 GN Amsterdam, Netherlands.
authors.forename: Tamara
authors.initials: T
authors.lastname: Peelen
authors.affiliation: RIKILT, Wageningen University and Research Centre, P.O. Box 230, 6700 AE Wageningen, Netherlands.
authors.forename: Esther
authors.identifier: 0000-0003-1472-6710
authors.initials: E
authors.lastname: Kok
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.
authors.forename: Jianxin
authors.identifier: 0000-0002-7717-0863
authors.initials: J
authors.lastname: Shi