title: Performance evaluation of Mindray CAL 8000(BC-6800 and SC-120) hematology analyzer and slidemaker/stainer.
abstract: BACKGROUND
  The Mindray CAL 8000 is a cellular analysis line that consists of the BC-6800, an automated hematology analyzer, and the SC-120, an automated slidemaker/stainer. We evaluated the performances of the BC-6800 and the SC-120.
  
  
  METHODS
  Four hundred and eight normal and abnormal samples were analyzed. The performance of the BC-6800 and Sysmex XE-2100 were compared, and blood films by the SC-120 and manual method were compared according to the CLSI guideline H26-A2 and H20-A2.
  
  
  RESULTS
  Most parameters measured by the BC-6800 matched well with the XE-2100 and manual differential. The flag efficiency of the BC-6800 for blasts (95.3%) and atypical lymphocytes (92.6%) were higher while immature granulocytes (89.7%) and NRBCs (94.1%) were lower than that of the XE-2100. Additionally, the BC-6800 detected four of five samples infected with plasmodium parasites. The SC-120 showed no carry-over and expected repeatability. There was good agreement on the five-part differential including abnormal cells between blood films by the SC-120 and manually prepared blood films. The shape of the RBC was also comparable between blood films.
  
  
  CONCLUSIONS
  The CAL-8000 analysis line is beneficial for precise, fast hematology work, and even more useful in malaria endemic areas.
authors.affiliation: Department of Laboratory Medicine, Gachon University Gil Medical Center, Incheon, Korea.
authors.forename: Hwan Tae
authors.identifier: http://orcid.org/0000-0002-5554-7877
authors.initials: HT
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Gachon University Gil Medical Center, Incheon, Korea.
authors.forename: Pil-Whan
authors.initials: PW
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, Gachon University Gil Medical Center, Incheon, Korea.
authors.forename: Yiel-Hea
authors.initials: YH
authors.lastname: Seo
authors.affiliation: Department of Laboratory Medicine, Gachon University Gil Medical Center, Incheon, Korea.
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Gachon University Gil Medical Center, Incheon, Korea.
authors.forename: Ja Young
authors.initials: JY
authors.lastname: Seo
authors.affiliation: Department of Laboratory Medicine, Gachon University Gil Medical Center, Incheon, Korea.
authors.forename: Ji-Hun
authors.initials: JH
authors.lastname: Jeong
authors.affiliation: Department of Laboratory Medicine, Gachon University Gil Medical Center, Incheon, Korea.
authors.forename: Moon Jin
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Gachon University Gil Medical Center, Incheon, Korea.
authors.forename: Jeong-Yeal
authors.initials: JY
authors.lastname: Ahn
mesh_terms: D001772:Blood Cell Count / Q000379:methods* / Q000592:standards*; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods* / Q000592:standards*
keywords: CAL 8000; automatic slidemaker/stainer; hematology analyzer; performance evaluation; peripheral blood films