title: Integrating microRNA and mRNA expression profiles of acute promyelocytic leukemia cells to explore the occurrence mechanisms of differentiation syndrome.
abstract: The pathogenesis of therapy-induced differentiation syndrome (DS) in patients with acute promyelocytic leukemia (APL) remains unclear. In this study, mRNA and microRNA (miRNA) expression profiling of peripheral blood APL cells from patients complicated with vs. without DS were integratively analyzed to explore the mechanisms underlying arsenic trioxide treatment-associated DS. By integrating the differentially expressed data with the data of differentially expressed microRNAs and their computationally predicted target genes, as well as the data of transcription factors and differentially expressed target microRNAs obtained from a literature search, a DS-related genetic regulatory network was constructed. Then using an EAGLE algorithm in clusterViz, the network was subdivided into 10 modules. Using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database the modules were annotated functionally, and three functionally active modules were recognized. The further in-depth analyses on the annotated functions of the three modules and the expression and roles of the related genes revealed that proliferation, differentiation, apoptosis and infiltration capability of APL cells might play important roles in the DS pathogenesis. The results could improve our understanding of DS pathogenesis from a more overall perspective, and could provide new clues for future research.
authors.affiliation: Central Laboratory, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Jinxiao
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ge
authors.affiliation: Central Laboratory, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Fenglin
authors.initials: F
authors.lastname: Cao
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.|Department of Neonatology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Xu
authors.affiliation: Central Laboratory, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Song
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Shuye
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Shengjin
authors.initials: S
authors.lastname: Fan
authors.affiliation: Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Li
authors.affiliation: Central Laboratory, The First Affiliated Hospital, Harbin Medical University, Harbin, China.|Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D045744:Cell Line, Tumor; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D053263:Gene Regulatory Networks; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D058977:Molecular Sequence Annotation; D012333:RNA, Messenger / Q000235:genetics*; D015203:Reproducibility of Results; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: acute promyelocytic leukemia; differentiation syndrome; mRNA; microRNA; microarrays