title: DNA integrity as biomarker in pancreatic cyst fluid.
abstract: Identification of pancreatic cysts with malignant potential is important to prevent pancreatic cancer development. Integrity of cell free DNA (cfDNA) has been described as tumor biomarker, but its potential for pancreatic cancer is unclear. While normal apoptotic cells release uniformly truncated DNA, malignant tissues release long fragments of cell free DNA (cfDNA). We measured 247 base pair (bp) and 115 bp DNA fragments of ALU repeats by qPCR in serum from healthy controls and pancreatic cancer patients, and in cyst fluid from pancreatic cyst patients. No differences in total cfDNA (ALU115) and cfDNA integrity (ALU247/115) were observed between sera from healthy controls (n=19) and pancreatic cancer patients (n=19). Although elevated as compared to serum, but no differences in cfDNA were found in cyst fluid from high risk (n=10) and low risk (n=20) cyst patients. We conclude that cfDNA integrity is not a useful marker to identify (pre)malignant pancreatic lesions.
authors.affiliation: Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, The Netherlands.
authors.forename: Wesley K
authors.initials: WK
authors.lastname: Utomo
authors.affiliation: Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, The Netherlands.
authors.forename: Vincent T
authors.initials: VT
authors.lastname: Janmaat
authors.affiliation: Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, The Netherlands.
authors.forename: Auke P
authors.initials: AP
authors.lastname: Verhaar
authors.affiliation: Department de Pathology, Hôpital Beaujon, INSERM U1149, Université Paris Diderot Clichy, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Cros
authors.affiliation: Service de Pancréatologie-Gastroentérologie, Pôle des Maladies de l'Appareil Digestif Université Denis Diderot-Paris VII Hôpital Beaujon Clichy Cedex, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lévy
authors.affiliation: Service de Pancréatologie-Gastroentérologie, Pôle des Maladies de l'Appareil Digestif Université Denis Diderot-Paris VII Hôpital Beaujon Clichy Cedex, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Ruszniewski
authors.affiliation: Department of Urology, Erasmus MC University Medical Center Rotterdam, The Netherlands.
authors.forename: Mirella S Vredenbregt-van
authors.initials: MS
authors.lastname: den Berg
authors.affiliation: Department of Urology, Erasmus MC University Medical Center Rotterdam, The Netherlands.
authors.forename: Guido
authors.initials: G
authors.lastname: Jenster
authors.affiliation: Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, The Netherlands.
authors.forename: Marco J
authors.initials: MJ
authors.lastname: Bruno
authors.affiliation: Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, The Netherlands.
authors.forename: Henri
authors.initials: H
authors.lastname: Braat
authors.affiliation: Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, The Netherlands.
authors.forename: Gwenny M
authors.initials: GM
authors.lastname: Fuhler
authors.affiliation: Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, The Netherlands.
authors.forename: Maikel P
authors.initials: MP
authors.lastname: Peppelenbosch
keywords: ALU repeats; Cell free DNA; PCR; circulating; pancreatic cyst fluid; serum