title: [Role of transient receptor potential vanilloid 1 in airway inflammation in asthmatic mice].
abstract: OBJECTIVE
  To study the effects of the change in transient receptor potential vanilloid 1 (TRPV1) channel activity on the degree of airway inflammation in asthmatic mice.
  
  
  METHODS
  BALB/c mice were randomly divided into control, asthma, capsaicin (TRPV1 agonist), capsazepine (TRPV1 antagonist), and dexamethasone groups. The asthmatic mouse model was established by intraperitoneal injection of mixed ovalbumin-aluminium hydroxide solution and ultrasonic atomization with OVA for sensitization and challenge. The capsaicin, capsazepine, and dexamethasone groups were given intraperitoneal injection of capsaicin (30 μg/kg), capsazepine (10 μmol/kg), and dexamethasone (2 mg/kg) respectively, at 30 minutes before challenge. Hematoxylin and eosin staining was used to observe the degree of pulmonary inflammation. ELISA was used to measure the content of interleukin-8 (IL-8) and interleukin-13 (IL-13) in bronchoalveolar lavage fluid (BALF). Real-Time PCR was used to measure the relative content of TRPV1 mRNA in lung tissue.
  
  
  RESULTS
  Compared with the asthma group, the capsazepine and dexamethasone groups showed reduced pulmonary inflammation, while the capsaicin group showed aggravated pulmonary inflammation. Compared with the control group, the asthma and capsaicin groups showed increases in the content of IL-13 and IL-8 in BALF and the mRNA expression of TRPV1 in lung tissue (P<0.05). Compared with the asthma group, the capsazepine and dexamethasone groups showed reductions in the content of IL-13 and IL-8 in BALF and the mRNA expression of TRPV1 in lung tissue (P<0.05). The capsaicin group showed increases in the content of IL-13 and IL-8 in BALF (P<0.05).
  
  
  CONCLUSIONS
  TRPV1 channel agonist and antagonist can influence the degree of airway inflammation in asthmatic mice. Dexamethasone may reduce airway inflammation through regulating TRPV1 level.
authors.affiliation: Department of Pediatrics, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. srm1975@sohu.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Feng
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Gao
authors.forename: Xu-Hua
authors.initials: XH
authors.lastname: Bian
authors.forename: Rui-Ming
authors.initials: RM
authors.lastname: Shi
mesh_terms: D000818:Animals; D001249:Asthma / Q000209:etiology*; D005260:Female; D018793:Interleukin-13 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis; D050916:TRPV Cation Channels / Q000235:genetics / Q000502:physiology*
chemical_list: D018793:Interleukin-13; D016209:Interleukin-8; D012333:RNA, Messenger; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse