title: A highly selective fluorescent probe based on coumarin for the imaging of N2H4in living cells.
abstract: A turn-on fluorescence probe for highly sensitive and selective detection of N2H4was developed based on hydrazine-triggered a substitution- cyclization-elimination cascade. Upon the treatment with N2H4, probe 1, 4-methyl-coumarin-7-yl bromobutanoate, displayed a remarkable fluorescence enhancement (25-fold) with a maximum at 450nm. This probe can quantitatively detect N2H4with a extremely low detection limit as 7×10-8M. Moreover, cell imaging experiments have indicated that probe 1 has potential ability to detect and image N2H4in biological systems.
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 333 Bukui Street, Qiqihar, Heilongjiang Province, PR China, 161006. Electronic address: chensongchemistry@163.com.
authors.forename: Song
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 333 Bukui Street, Qiqihar, Heilongjiang Province, PR China, 161006.
authors.forename: Peng
authors.initials: P
authors.lastname: Hou
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 333 Bukui Street, Qiqihar, Heilongjiang Province, PR China, 161006.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 333 Bukui Street, Qiqihar, Heilongjiang Province, PR China, 161006.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Qiqihar Medical University, 333 Bukui Street, Qiqihar, Heilongjiang Province, PR China, 161006.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000838:Anions / Q000737:chemistry; D003374:Coumarins / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006834:Hydrazines / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D013997:Time Factors; D014867:Water / Q000032:analysis / Q000737:chemistry
chemical_list: D000838:Anions; D003374:Coumarins; D005456:Fluorescent Dyes; D006834:Hydrazines; D014867:Water; C029424:hydrazine; C030123:coumarin
keywords: Cell imaging; Coumarin; Fluorescent probe; N(2)H(4)