title: Nucleophile sensitivity ofDrosophilaTRPA1 underlies light-induced feeding deterrence.
abstract: Solar irradiation including ultraviolet (UV) light causes tissue damage by generating reactive free radicals that can be electrophilic or nucleophilic due to unpaired electrons. Little is known about how free radicals induced by natural sunlight are rapidly detected and avoided by animals. We discover thatDrosophilaTransient Receptor Potential Ankyrin 1 (TRPA1), previously known only as an electrophile receptor, sensitively detects photochemically active sunlight through nucleophile sensitivity. Rapid light-dependent feeding deterrence inDrosophilawas mediated only by the TRPA1(A) isoform, despite the TRPA1(A) and TRPA1(B) isoforms having similar electrophile sensitivities. Such isoform dependence re-emerges in the detection of structurally varied nucleophilic compounds and nucleophilicity-accompanying hydrogen peroxide (H2O2). Furthermore, these isoform-dependent mechanisms require a common set of TRPA1(A)-specific residues dispensable for electrophile detection. Collectively, TRPA1(A) rapidly responds to natural sunlight intensities through its nucleophile sensitivity as a receptor of photochemically generated radicals, leading to an acute light-induced behavioral shift inDrosophila.
authors.affiliation: Samsung Biomedical Research Institute, Seoul, Republic of Korea.|Department of Anatomy and Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
authors.forename: Eun Jo
authors.initials: EJ
authors.lastname: Du
authors.affiliation: Samsung Biomedical Research Institute, Seoul, Republic of Korea.|Department of Anatomy and Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Ahn
authors.affiliation: Samsung Biomedical Research Institute, Seoul, Republic of Korea.|Department of Physiology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
authors.forename: Xianlan
authors.initials: X
authors.lastname: Wen
authors.affiliation: Samsung Biomedical Research Institute, Seoul, Republic of Korea.|Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.|Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea.
authors.forename: Dae-Won
authors.initials: DW
authors.lastname: Seo
authors.affiliation: Samsung Biomedical Research Institute, Seoul, Republic of Korea.|Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.|Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea.
authors.forename: Duk L
authors.initials: DL
authors.lastname: Na
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon, Republic of Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Kwon
authors.affiliation: Department of Biomedical Engineering, Sungkyunkwan University, Suwon, Republic of Korea.|Center for Neuroscience Imaging Research, Institute for Basic Science, Suwon, Republic of Korea.
authors.forename: Myunghwan
authors.initials: M
authors.lastname: Choi
authors.affiliation: College of Life Sciences, Sejong University, Seoul, Republic of Korea.
authors.forename: Hyung-Wook
authors.initials: HW
authors.lastname: Kim
authors.affiliation: Samsung Biomedical Research Institute, Seoul, Republic of Korea.|Department of Physiology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
authors.forename: Hana
authors.identifier: 0000-0002-9394-8671
authors.initials: H
authors.lastname: Cho
authors.affiliation: Samsung Biomedical Research Institute, Seoul, Republic of Korea.|Department of Anatomy and Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
authors.forename: KyeongJin
authors.identifier: 0000-0003-0446-469X
authors.initials: K
authors.lastname: Kang
mesh_terms: D000818:Animals; D004330:Drosophila / Q000502:physiology* / Q000528:radiation effects*; D029721:Drosophila Proteins / Q000378:metabolism*; D005247:Feeding Behavior / Q000528:radiation effects; D005609:Free Radicals / Q000378:metabolism; D007473:Ion Channels; D013472:Sunlight; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D005609:Free Radicals; D007473:Ion Channels; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels; C475684:TrpA1 protein, Drosophila
keywords: D. melanogaster; TRPA1; chemical nociception; feeding deterrence; free radicals; neuroscience; nucleophiles; photobiology; photochemistry