title: Integrative analysis of signaling pathways and diseases associated with the miR-106b/25 cluster and their function study in berb﻿erine-induced multiple myeloma cells.
abstract: Berberine (BBR), a traditional Chinese herbal medicine compound, has emerged as a novel class of anti-tumor agent. Our previous microRNA (miRNA) microarray demonstrated that miR-106b/25 was significantly down-regulated in BBR-treated multiple myeloma (MM) cells. Here, systematic integration showed that miR-106b/25 cluster is involved in multiple cancer-related signaling pathways and tumorigenesis. MiREnvironment database revealed that multiple environmental factors (drug, ionizing radiation, hypoxia) affected the miR-106b/25 cluster expression. By targeting the seed region in the miRNA, tiny anti-mir106b/25 cluster (t-anti-mir106b/25 cluster) significantly induced suppression in cell viability and colony formation. Western blot validated that t-anti-miR-106b/25 cluster effectively inhibited the expression of P38 MAPK and phospho-P38 MAPK in MM cells. These findings indicated the miR-106b/25 cluster functioned as oncogene and might provide a novel molecular insight into MM.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Jinan University, 601 Western Huangpu Avenue, 510632, Guangzhou, China.|Institute of Chinese Integrative Medicine, Medical College of Jinan University, Guangzhou, 510632, China.
authors.forename: Chunming
authors.initials: C
authors.lastname: Gu
authors.affiliation: Department of Clinical Medicine, Medical College of Jinan University, Guangzhou, 510632, China.
authors.forename: Tianfu
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Jinan University, 601 Western Huangpu Avenue, 510632, Guangzhou, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of Blood Transfusion, The First Affiliated Hospital of Jinan University, Guangzhou, 510632, China.
authors.forename: Shengting
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Jinan University, 601 Western Huangpu Avenue, 510632, Guangzhou, China. efeijia@163.com.|Institute of Chinese Integrative Medicine, Medical College of Jinan University, Guangzhou, 510632, China. efeijia@163.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Fei
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310006, China. sjping88@163.com.
authors.forename: Jianping
authors.initials: J
authors.lastname: Shen
authors.affiliation: Institute of Hematology, Medical College of Jinan University, Guangzhou, 510632, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001599:Berberine / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009101:Multiple Myeloma / Q000473:pathology*; D015398:Signal Transduction
chemical_list: C542541:MIRN106 microRNA, human; D035683:MicroRNAs; D001599:Berberine
keywords: Berberine; MiR-106b/25 cluster; Multiple myeloma; Signaling pathways; p38 MAPK