title: Novel yeast isolated from broilers' feedstuff, gut and faeces as aflatoxin B1adsorbents.
abstract: OBJECTIVE
  To isolate and characterize native yeast strains from broilers' environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1(AFB1).
  
  
  RESULTS
  A total of nine yeast strains were isolated: three from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), two from gut identified as Candida tropicalis and four from faeces identified as Cl. lusitaniae (3) and Cyberlindnera fabianii (1). AFB1binding percentages varied among yeast strains and with AFB1concentrations. To carry out adsorption studies, one strain from each genus and each origin was selected as follows: Cl. lusitaniae and P. kudriavzevii from feedstuff, Cl. lusitaniae and Cy. fabianii from faeces and Ca. tropicalis from gut. The most appropriate concentrations for cells and toxin were 107cells per ml and 100 ng ml-1of AFB1respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherm studies in all yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the AFB1-yeast complex demonstrated the irreversibility of the binding process.
  
  
  CONCLUSIONS
  Yeast strains tested in this study constitute potential AFB1adsorbents and they possess the advantage to be native from the avian environment.
  
  
  CONCLUSIONS
  This study makes a contribution to using native yeasts from broilers' environment for controlling chronic aflatoxicosis in avian production.
authors.affiliation: Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.|Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires (CABA), Argentina.
authors.forename: A P
authors.initials: AP
authors.lastname: Magnoli
authors.affiliation: Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors.forename: M C
authors.initials: MC
authors.lastname: Rodriguez
authors.affiliation: Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors.forename: V L
authors.initials: VL
authors.lastname: Poloni
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria (INTA), EEA Mendoza, Lujan de Cuyo, Mendoza, Argentina.
authors.forename: M C
authors.initials: MC
authors.lastname: Rojo
authors.affiliation: Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires (CABA), Argentina.|Instituto Nacional de Tecnología Agropecuaria (INTA), EEA Mendoza, Lujan de Cuyo, Mendoza, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Combina
authors.affiliation: Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires (CABA), Argentina.|Departamento de Química, Facultad de Ciencias Exactas, Físico, Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors.forename: S M
authors.initials: SM
authors.lastname: Chiacchiera
authors.affiliation: Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.|Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires (CABA), Argentina.
authors.forename: A M
authors.initials: AM
authors.lastname: Dalcero
authors.affiliation: Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.|Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires (CABA), Argentina.
authors.forename: L R
authors.initials: LR
authors.lastname: Cavaglieri
mesh_terms: D000327:Adsorption; D016604:Aflatoxin B1 / Q000378:metabolism*; D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D002645:Chickens / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D007422:Intestines / Q000382:microbiology; D015003:Yeasts / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D016604:Aflatoxin B1
keywords: adsorption; aflatoxin B1; broilers’ ecosystem; native; yeast