title: Effect of UV Irradiation on the Nutritional Quality and Cytotoxicity of Apple Juice.
abstract: UV-C irradiation operating at 254 nm wavelength on the polyphenolic and vitamin contents of apple juice including cytotoxicity analysis was studied. UV doses ranging from 0 to 150 mJ·cm-2were selected for the treatments. Polyphenols (catechin, epicatechin, chlorogenic acid, and phloridzin) and vitamins (riboflavin, thiamine hydrochloride, pyridoxal hydrochloride, pyridoxine, pyridoxamine dihydrochloride, cyanocobalamin, choline chloride, biotin, niacin, and niacinamide) were chemically profiled. It was observed that UV treatment of apple juice at disinfection doses caused minor reductions (p < 0.05) in the concentrations of two main polyphenols (i.e., chlorogenic acid and epicatechin). In contrast, significant (p < 0.05) decreases in vitamin concentrations were observed (p < 0.05). The irradiated juice was evaluated for cytotoxic effects. The irradiated apple juice showed no cytotoxic effects on normal intestinal cells, and both irradiated and nonirradiated samples are significantly comparable in inhibiting the growth of human colon cancer cells. Overall, these results indicated that UV-C treatment of apple juice neither significantly degraded polyphenols nor generated cytotoxic compounds.
authors.affiliation: Department of Agricultural and Environmental Sciences, Tennessee State University , Nashville, Tennessee 37209, United States.
authors.forename: M Shajedul
authors.initials: MS
authors.lastname: Islam
authors.affiliation: Department of Agricultural and Environmental Sciences, Tennessee State University , Nashville, Tennessee 37209, United States.
authors.forename: A
authors.initials: A
authors.lastname: Patras
authors.affiliation: Department of Agricultural and Environmental Sciences, Tennessee State University , Nashville, Tennessee 37209, United States.
authors.forename: B
authors.initials: B
authors.lastname: Pokharel
authors.affiliation: Department of Pharmaceutical Sciences and Department of Chemistry & Biochemistry, Lipscomb University , Nashville, Tennessee 37204, United States.
authors.forename: M J
authors.initials: MJ
authors.lastname: Vergne
authors.affiliation: Aquafine Corporation, Valencia, California 91355, United States.
authors.forename: M
authors.initials: M
authors.lastname: Sasges
authors.affiliation: Aquafine Corporation, Valencia, California 91355, United States.
authors.forename: A
authors.initials: A
authors.lastname: Begum
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Kanyasiri
authors.initials: K
authors.lastname: Rakariyatham
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Che
authors.initials: C
authors.lastname: Pan
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: H
authors.initials: H
authors.lastname: Xiao
keywords: UV-C; cytotoxicity; dosage; irradiation; kinetics; vitamins