title: Evaluation of Genotoxic Pressure along the Sava River.
abstract: In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
authors.affiliation: University of Belgrade, Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Belgrade, Serbia.
authors.forename: Stoimir
authors.initials: S
authors.lastname: Kolarević
authors.affiliation: University of Belgrade, Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Belgrade, Serbia.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Aborgiba
authors.affiliation: University of Belgrade, Institute for Biological Research ¨Siniša Stanković¨, Belgrade, Serbia.
authors.forename: Margareta
authors.initials: M
authors.lastname: Kračun-Kolarević
authors.affiliation: University of Belgrade, Institute for Multidisciplinary Research, Belgrade, Serbia.
authors.forename: Jovana
authors.initials: J
authors.lastname: Kostić
authors.affiliation: University of Belgrade, Faculty of Biology, Institute of Zoology, Belgrade, Serbia.
authors.forename: Predrag
authors.initials: P
authors.lastname: Simonović
authors.affiliation: University of Kragujevac, Faculty of Science, Kragujevac, Serbia.
authors.forename: Vladica
authors.initials: V
authors.lastname: Simić
authors.affiliation: University of Kragujevac, Faculty of Science, Kragujevac, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Milošković
authors.affiliation: TU Wien, Institute for Chemical Engineering, Research Group Environmental Microbiology and Molecular Ecology, Interuniversity Cooperation Centre Water & Health, Vienna, Austria.
authors.forename: Georg
authors.initials: G
authors.lastname: Reischer
authors.affiliation: TU Wien, Institute for Chemical Engineering, Research Group Environmental Microbiology and Molecular Ecology, Interuniversity Cooperation Centre Water & Health, Vienna, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Farnleitner
authors.affiliation: University of Belgrade, Institute for Multidisciplinary Research, Belgrade, Serbia.
authors.forename: Zoran
authors.initials: Z
authors.lastname: Gačić
authors.affiliation: Department of Environmental Sciences, Jožef Stefan Institute, Ljubljana, Slovenia.
authors.forename: Radmila
authors.initials: R
authors.lastname: Milačič
authors.affiliation: Department of Environmental Sciences, Jožef Stefan Institute, Ljubljana, Slovenia.
authors.forename: Tea
authors.initials: T
authors.lastname: Zuliani
authors.affiliation: Department of Environmental Sciences, Jožef Stefan Institute, Ljubljana, Slovenia.
authors.forename: Janja
authors.initials: J
authors.lastname: Vidmar
authors.affiliation: University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Belgrade, Serbia.
authors.forename: Marija
authors.initials: M
authors.lastname: Pergal
authors.affiliation: University of Zagreb, Faculty of Agriculture, Zagreb, Croatia.
authors.forename: Marina
authors.initials: M
authors.lastname: Piria
authors.affiliation: University of Belgrade, Institute for Biological Research ¨Siniša Stanković¨, Belgrade, Serbia.
authors.forename: Momir
authors.initials: M
authors.lastname: Paunović
authors.affiliation: University of Belgrade, Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Belgrade, Serbia.
authors.forename: Branka
authors.initials: B
authors.lastname: Vuković-Gačić
mesh_terms: D000818:Animals; D005060:Europe; D005399:Fishes; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D045483:Rivers; D013014:SOS Response, Genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D009153:Mutagens; D014874:Water Pollutants, Chemical