title: 13-Methyl-palmatrubine induces apoptosis and cell cycle arrest in A549 cells in vitro and in vivo.
abstract: Corydalis yanhusuo, a well-known herbaceous plant, is commonly used in the treatment of inflammation, injury and pain. One natural agent isolated from Corydalis yanhusuo, 13-methyl-palmatrubine, was found to have a cytotoxic effect on cancer cells as reported in published studies. In the present study, we synthesized a potential anti-lung tumor agent, 13-methyl-palmatrubine and analyzed its activity. 13-Methyl-palmatrubine exhibited a cytotoxic effect on a panel of cancer cell lines in a time- and concentration-dependent manner. Among all the tested cancer cell lines, lung cancer A549 cells were most sensitive to 13-methyl-palmatrubine treatment. Meanwhile 13-methyl-palmatrubine showed less cytotoxicity in human normal cells. Our investigation revealed that 13-methyl‑palmatrubine induced apoptosis and cell cycle arrest in A549 cells in a dose-dependent manner. Furthermore, 13-methyl-palmatrubine treatment caused activation of P38 and JNK pathways and blocked the EGFR pathway. In conclusion, our findings demonstrated that 13-methyl-palmatrubine inhibited the growth of A549 cells mediated by blocking of the EGFR signaling pathway and activation of the MAPK signaling pathway and provides a better understanding of the molecular mechanisms of 13-methyl-palmatrubine.
authors.affiliation: Department of Traditional Chinese Medicine, RuiJin Hospital, JiaoTong University School of Medicine, Shanghai 200025, P.R. China.
authors.forename: Jingxian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Traditional Chinese Medicine, HuaDong Hospital, FuDan University School of Medicine, Shanghai 200040, P.R. China.
authors.forename: Xingang
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Respiration, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, P.R. China.
authors.forename: Chenghua
authors.initials: C
authors.lastname: Lu
authors.affiliation: Department of Oncology, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, P.R. China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Traditional Chinese Medicine, HuaDong Hospital, FuDan University School of Medicine, Shanghai 200040, P.R. China.
authors.forename: Haizhu
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Traditional Chinese Medicine, HuaDong Hospital, FuDan University School of Medicine, Shanghai 200040, P.R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Traditional Chinese Medicine, HuaDong Hospital, FuDan University School of Medicine, Shanghai 200040, P.R. China.
authors.forename: Haixin
authors.initials: H
authors.lastname: Gou
authors.affiliation: Department of Traditional Chinese Medicine, HuaDong Hospital, FuDan University School of Medicine, Shanghai 200040, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Clinical Medicine College, Shanghai University of Medicine and Health Sciences, Shanghai 201318, P.R. China.
authors.forename: Wangchun
authors.initials: W
authors.lastname: Du
mesh_terms: D000072283:A549 Cells; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001600:Berberine Alkaloids / Q000008:administration & dosage*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D066246:ErbB Receptors / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D023041:Xenograft Model Antitumor Assays; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics
chemical_list: C539140:13-methyl-palmatrubine; D001600:Berberine Alkaloids; C512478:EGFR protein, human; D066246:ErbB Receptors; D048051:p38 Mitogen-Activated Protein Kinases