title: The Pil3 pilus of Streptococcus gallolyticus binds to intestinal mucins and to fibrinogen.
abstract: Streptococcus gallolyticus is a commensal bacterium responsible for infectious endocarditis in the elderly, which has frequently been associated with colonic carcinoma. Whether this species is a cause or a consequence of colorectal cancer remains unknown. We recently demonstrated that S. gallolyticus Pil3 pilus is required for adhesion to colonic mucus and for colonization of mouse distal colon. We show here that Pil3 pilus binds equally well to human colonic mucins derived from HT29-MTX cells and to human stomach mucins from healthy donors. In addition, we have found that Pil3 also binds to human fibrinogen, which expands the repertoire of Pil3 host ligands.
authors.affiliation: a Department of Microbiology , Biology of Gram-positive Pathogens Unit, Institut Pasteur , Paris , France.|b Department of Microbiology , Center National de la Recherche Scientifique (CNRS) ERL3526 , Paris , France.
authors.forename: Mariana
authors.initials: M
authors.lastname: Martins
authors.affiliation: a Department of Microbiology , Biology of Gram-positive Pathogens Unit, Institut Pasteur , Paris , France.|b Department of Microbiology , Center National de la Recherche Scientifique (CNRS) ERL3526 , Paris , France.
authors.forename: Constance
authors.initials: C
authors.lastname: Porrini
authors.affiliation: a Department of Microbiology , Biology of Gram-positive Pathogens Unit, Institut Pasteur , Paris , France.|b Department of Microbiology , Center National de la Recherche Scientifique (CNRS) ERL3526 , Paris , France.
authors.forename: Laurence
authors.initials: L
authors.lastname: du Merle
authors.affiliation: a Department of Microbiology , Biology of Gram-positive Pathogens Unit, Institut Pasteur , Paris , France.|b Department of Microbiology , Center National de la Recherche Scientifique (CNRS) ERL3526 , Paris , France.
authors.forename: Camille
authors.initials: C
authors.lastname: Danne
authors.affiliation: c University Lille Nord , USTL , Villeneuve d'Ascq , France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Robbe-Masselot
authors.affiliation: a Department of Microbiology , Biology of Gram-positive Pathogens Unit, Institut Pasteur , Paris , France.|b Department of Microbiology , Center National de la Recherche Scientifique (CNRS) ERL3526 , Paris , France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Trieu-Cuot
authors.affiliation: a Department of Microbiology , Biology of Gram-positive Pathogens Unit, Institut Pasteur , Paris , France.|b Department of Microbiology , Center National de la Recherche Scientifique (CNRS) ERL3526 , Paris , France.
authors.forename: Shaynoor
authors.initials: S
authors.lastname: Dramsi
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D005340:Fibrinogen / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D051379:Mice; D009077:Mucins / Q000378:metabolism*; D013290:Streptococcal Infections / Q000378:metabolism* / Q000382:microbiology*; D000070099:Streptococcus gallolyticus / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D009077:Mucins; D036781:Fimbriae Proteins; D005340:Fibrinogen
keywords: MUC2; MUC5AC; Pil3 pilus; S. bovis; S. gallolyticus; colon; colonization; fibrinogen; mucins