title: Anti-Inflammatory Effects of Hyperbaric Oxygenation during DSS-Induced Colitis in BALB/c Mice Include Changes in Gene Expression ofHIF-1α, Proinflammatory Cytokines, and Antioxidative Enzymes.
abstract: Reactive oxygen species (ROS) and nitrogen species have an indispensable role in regulating cell signalling pathways, including transcriptional control via hypoxia inducible factor-1α(HIF-1α). Hyperbaric oxygenation treatment (HBO2) increases tissue oxygen content and leads to enhanced ROS production. In the present study DSS-induced colitis has been employed in BALB/c mice as an experimental model of gut mucosa inflammation to investigate the effects of HBO2onHIF-1α, antioxidative enzyme, and proinflammatory cytokine genes during the colonic inflammation. Here we report that HBO2significantly reduces severity of DSS-induced colitis, as evidenced by the clinical features, histological assessment, impaired immune cell expansion and mobilization, and reversal ofIL-1β,IL-2, andIL-6gene expression. Gene expression and antioxidative enzyme activity were changed by the HBO2and the inflammatory microenvironment in the gut mucosa. Strong correlation ofHIF-1αmRNA level toGPx1,SOD1, andIL-6mRNA expression suggests involvement ofHIF-1αin transcriptional regulation of these genes during colonic inflammation and HBO2. This is further confirmed by a strong correlation ofHIF-1αwith known target genes VEGF andPGK1. Results demonstrate that HBO2has an anti-inflammatory effect in DSS-induced colitis in mice, and this effect is at least partly dependent on expression ofHIF-1αand antioxidative genes.
authors.affiliation: Department of Physiology and Immunology, Faculty of Medicine, University of Osijek, 31 000 Osijek, Croatia.
authors.forename: Sanja
authors.identifier: 0000-0002-3380-8001
authors.initials: S
authors.lastname: Novak
authors.affiliation: Department of Physiology and Immunology, Faculty of Medicine, University of Osijek, 31 000 Osijek, Croatia.
authors.forename: Ines
authors.identifier: 0000-0003-4964-7721
authors.initials: I
authors.lastname: Drenjancevic
authors.affiliation: Department of Biology, University of Osijek, 31 000 Osijek, Croatia.
authors.forename: Rosemary
authors.identifier: 0000-0002-2324-3617
authors.initials: R
authors.lastname: Vukovic
authors.affiliation: Department of Immunology and Biotechnology, Faculty of Medicine, University of Pécs, 7624 Pécs, Hungary; Lymphoid Organogenesis Research Group, Szentágothai Research Center, University of Pécs, 7624 Pécs, Hungary.
authors.forename: Zoltán
authors.identifier: 0000-0001-9560-6424
authors.initials: Z
authors.lastname: Kellermayer
authors.affiliation: Department of Physiology and Immunology, Faculty of Medicine, University of Osijek, 31 000 Osijek, Croatia.
authors.forename: Anita
authors.identifier: 0000-0002-3374-8512
authors.initials: A
authors.lastname: Cosic
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, University of Osijek, 31 000 Osijek, Croatia.
authors.forename: Maja
authors.identifier: 0000-0002-0968-4926
authors.initials: M
authors.lastname: Tolusic Levak
authors.affiliation: Department of Immunology and Biotechnology, Faculty of Medicine, University of Pécs, 7624 Pécs, Hungary; Lymphoid Organogenesis Research Group, Szentágothai Research Center, University of Pécs, 7624 Pécs, Hungary.
authors.forename: Péter
authors.identifier: 0000-0001-5749-4775
authors.initials: P
authors.lastname: Balogh
authors.affiliation: Department of Physiology and Immunology, Faculty of Medicine, University of Osijek, 31 000 Osijek, Croatia.
authors.forename: Filip
authors.identifier: 0000-0002-2016-4526
authors.initials: F
authors.lastname: Culo
authors.affiliation: Department of Physiology and Immunology, Faculty of Medicine, University of Osijek, 31 000 Osijek, Croatia.
authors.forename: Martina
authors.identifier: 0000-0002-5734-5849
authors.initials: M
authors.lastname: Mihalj