title: Long non-coding RNA H19 enhances cell proliferation and anchorage-independent growth of cervical cancer cell lines.
abstract: Long non-coding RNA H19 is aberrantly expressed in multiple malignancies and its expression levels correlate with recurrence, metastasis, and patient survival. Despite numerous reports documenting the role of H19 in carcinogenesis, its contribution to cervical cancer development is still largely unknown. In this study, I observed that H19 expression was elevated in cervical cancer cell lines and could be detected in extracellular vesicles in the culture medium. In addition, I demonstrated, by overexpression and knockdown experiments, that H19 promoted cell proliferation and multicellular tumor spheroid formation without significantly affecting apoptosis and cell migration. Finally, treatment with transforming growth factor beta and hypoxia-mimetic CoCl2could modulate H19 levels in a cell line-specific manner. These findings indicate that H19 promotes both anchorage-specific and -independent growth of cervical cancer cell lines and may serve as a potential target for cancer diagnosis and therapy.
authors.affiliation: National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathum Thani 12120, Thailand.
authors.forename: Tawin
authors.initials: T
authors.lastname: Iempridee
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D063646:Carcinogenesis / Q000235:genetics; D002448:Cell Adhesion / Q000235:genetics; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D003035:Cobalt / Q000494:pharmacology; D000067128:Extracellular Vesicles / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D018874:Spheroids, Cellular / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C094335:H19 long non-coding RNA; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; D016212:Transforming Growth Factor beta; D003035:Cobalt; C018021:cobaltous chloride
keywords: H19; cervical cancer; extracellular vesicles; hypoxia; lncRNA; transforming growth factor beta