title: Influence of culture conditions for clinically isolated non-albicans Candida biofilm formation.
abstract: Non-albicans Candida species have been isolated in increasing numbers in patients. Moreover, they are adept at forming biofilms. This study analyzed biofilm formation of clinically isolated non-albicans Candida, including Candida tropicalis, Candida krusei and Candida parapsilosis under the influence of different growth media (RPMI 1640, YPD and BHI) and several culture variables (inoculum concentration, incubation period and feeding conditions). The results showed that culture conditions strongly influenced non-albicans Candida species biofilm formation. YPD and BHI resulted in larger amount of biofilm formation with higher metabolic activity of biofilms. Furthermore, the growth media seems to have varying effects on adhesion and biofilm development. Growth conditions may also influence biofilm formation, which was enhanced when starting the culture with a larger inoculum, longer incubation period and using a fed-batch system. Therefore, the potential influences of external environmental factors should be considered when studying the non-albicans Candida biofilms in vitro.
authors.affiliation: Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.. Electronic address: yulong.tan@meduniwien.ac.at.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.. Electronic address: matthias.leonhard@meduniwien.ac.at.
authors.forename: Matthias
authors.initials: M
authors.lastname: Leonhard
authors.affiliation: Food Biotechnology Laboratory, Department of Food Sciences and Technology, BOKU-University of Natural Resources and Life Sciences, 1190 Vienna, Austria. Electronic address: masu277@gmail.com.
authors.forename: Su
authors.initials: S
authors.lastname: Ma
authors.affiliation: Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.. Electronic address: berit.schneider-stickler@meduniwien.ac.at.
authors.forename: Berit
authors.initials: B
authors.lastname: Schneider-Stickler
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods; D018441:Biofilms / Q000254:growth & development*; D002175:Candida / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification* / Q000378:metabolism; D002448:Cell Adhesion; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D007831:Larynx, Artificial / Q000382:microbiology
chemical_list: D003470:Culture Media
keywords: Biofilm; Clinical isolates; Non-albicans Candida species