title: Streptozotocin inhibits synaptic transmission and edaravone attenuates streptozotocin-induced electrophysiological changes in CA1 pyramidal neurons of rat hippocampal slices.
abstract: The purpose of this study was to investigate the acute and chronic effects of streptozotocin (STZ) upon synaptic transmission and the effects of edaravone (EDA, a free radical scavenger) on STZ-induced electrophysiological changes in CA1 pyramidal neurons of rat hippocampal slices. To accomplish this goal, spontaneous excitatory postsynaptic current (sEPSC), miniature excitatory postsynaptic current (mEPSC), spontaneous inhibitory postsynaptic current (sIPSC) and miniature inhibitory postsynaptic current (mIPSC) were recorded within hippocampal slices using whole-cell patch clamp techniques. The results showed that the amplitudes and frequencies of sEPSC, mEPSC, sIPSC and mIPSC were inhibited by 1000μM STZ, while treatment of EDA (1000μM) attenuated these STZ-induced changes. The degree of these neurotoxic effects of STZ and effects of EDA increased as a function of drug duration as assessed at 2, 4 or 8h of exposure. Taken together, our results demonstrate that STZ induces neurotoxicity within these hippocampal slices through its capacity to alter synaptic transmission and these STZ-induced alterations in electrophysiological responses are attenuated by EDA.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, 23 Post Street, Harbin 150001, PR China. Electronic address: jutingxiyuan@126.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Ju
authors.affiliation: Department of Physiology, Harbin Medical University, PR China. Electronic address: wangxr98146@163.com.
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, 23 Post Street, Harbin 150001, PR China. Electronic address: zhouss613008@126.com.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, 23 Post Street, Harbin 150001, PR China. Electronic address: ztt_1984@163.com.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Department of Neurology, The Fourth Affiliated Hospital of Harbin Medical University, PR China. Electronic address: ymm048048@163.com.
authors.forename: Meimei
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, 23 Post Street, Harbin 150001, PR China. Electronic address: linjinghan1986@126.com.
authors.forename: Jinghan
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, 23 Post Street, Harbin 150001, PR China. Electronic address: linamoon1@163.com.
authors.forename: Lina
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, 23 Post Street, Harbin 150001, PR China. Electronic address: ltj_1989@hotmail.com.
authors.forename: Tingjiao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, 23 Post Street, Harbin 150001, PR China. Electronic address: xuyi198861@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, 23 Post Street, Harbin 150001, PR China. Electronic address: zfx0001@126.com.
authors.forename: Liming
authors.initials: L
authors.lastname: Zhang
mesh_terms: D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D056547:CA1 Region, Hippocampal / Q000166:cytology*; D004305:Dose-Response Relationship, Drug; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D018408:Patch-Clamp Techniques; D017966:Pyramidal Cells / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin / Q000494:pharmacology*; D009435:Synaptic Transmission / Q000187:drug effects*; D013997:Time Factors; D014633:Valine / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000903:Antibiotics, Antineoplastic; D018691:Excitatory Amino Acid Antagonists; D013311:Streptozocin; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; C056639:2-amino-5-phosphopentanoic acid; D014633:Valine
keywords: EPSC; Edaravone; IPSC; Pyramidal neuron; Slice patch clamp; Streptozotocin