title: The RNA polymerase I subunit Rpa12p interacts with the stress-responsive transcription factor Msn4p to regulate lipid metabolism in budding yeast.
abstract: In Saccharomyces cerevisiae, RPA12 encodes the small subunit of RNA polymerase I. Here, we demonstrate that Rpa12p interacts with the transcription factor Msn4p and prevents its binding to the promoter of AYR1 encoding Ayr1p (1-acyldihydroxyacetone phosphate reductase), a key enzyme involved in triacylglycerol biosynthesis and mobilization of nonpolar lipids. Deletion of RPA12 leads to triacylglycerol accumulation due to the binding of Msn4p to the promoter of AYR1 and activation of its transcription. The double deletion rpa12Δ::ayr1Δ caused a reduction in triacylglycerol levels. Our findings reveal that Rpa12p functions as a negative regulator of lipid metabolism by modulating nonpolar lipid biosynthesis through its interaction with Msn4p.
authors.affiliation: Lipidomics Centre, Department of Lipid Science, Council of Scientific and Industrial Research (CSIR) - Central Food Technological Research Institute (CFTRI), Mysore, Karnataka, India.|Academy of Scientific and Innovative Research, CSIR-CFTRI, Mysore, Karnataka, India.
authors.forename: Kamlesh Kumar
authors.initials: KK
authors.lastname: Yadav
authors.affiliation: Lipidomics Centre, Department of Lipid Science, Council of Scientific and Industrial Research (CSIR) - Central Food Technological Research Institute (CFTRI), Mysore, Karnataka, India.|Academy of Scientific and Innovative Research, CSIR-CFTRI, Mysore, Karnataka, India.
authors.forename: Neelima
authors.initials: N
authors.lastname: Singh
authors.affiliation: Lipidomics Centre, Department of Lipid Science, Council of Scientific and Industrial Research (CSIR) - Central Food Technological Research Institute (CFTRI), Mysore, Karnataka, India.|Academy of Scientific and Innovative Research, CSIR-CFTRI, Mysore, Karnataka, India.
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Rajvanshi
authors.affiliation: Lipidomics Centre, Department of Lipid Science, Council of Scientific and Industrial Research (CSIR) - Central Food Technological Research Institute (CFTRI), Mysore, Karnataka, India. ram@cftri.com.|Academy of Scientific and Innovative Research, CSIR-CFTRI, Mysore, Karnataka, India. ram@cftri.com.
authors.forename: Ram
authors.initials: R
authors.lastname: Rajasekharan
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D050356:Lipid Metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012318:RNA Polymerase I / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C081937:MSN4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D014280:Triglycerides; C423311:RPA12 protein, S cerevisiae; D012318:RNA Polymerase I; C586371:Ayr1 protein, S cerevisiae; D002265:Carboxylic Ester Hydrolases
keywords: nonpolar lipid metabolism; transcriptional regulation; triacylglycerol