title: Investigation of association between IL-8 serum levels and IL8 polymorphisms in Chinese patients with sepsis.
abstract: OBJECTIVE
  To assess the clinical relevance of IL8 gene polymorphisms in patients with sepsis and its association with systemic IL-8 levels.
  
  
  METHODS
  PCR and DNA sequencing were used to examine the polymorphism of IL8 in 152 patients with sepsis and in 199 healthy volunteers in China. The distribution frequencies of the genotype and allele were compared among different groups. The serum IL-8 was measured by ELISA and analyzed in relation to polymorphisms of IL8.
  
  
  RESULTS
  The homozygote TT genotype and T allele of rs4073 (genotype: p=0.01, allele: p=0.002), the homozygote CC genotype and C allele (genotype: p=0.03, allele: p=0.003) of rs2227306, homozygote AA genotype and A allele of re1126647 (genotype: p=0.01, allele: p=0.002) were associated with susceptibility to sepsis in males. Serum IL-8 levels were significantly increased in patients with sepsis but showed no correlation with IL8 rs4073, rs2227306 and rs1126647 polymorphisms.
  
  
  CONCLUSIONS
  The male population carrying the homozygote TT genotype and T allele of rs4073, the homozygote CC genotype and C allele of rs2227306 and homozygote AA genotype and A allele of rs1126647 are more susceptible to sepsis, suggesting there is a protective effect in females carrying these genotypes and alleles respectively. There was no association between rs4073, rs2227306 and rs1126647 polymorphisms and serum levels of IL-8 in patients with sepsis.
authors.affiliation: Hubei Cancer Hospital, No. 116 Zhuodaoquan South Road, Hongshan District, Wuhan 430079, Hubei, PR China. Electronic address: syhdh-007@163.com.
authors.forename: Donghai
authors.initials: D
authors.lastname: Hu
authors.affiliation: Guangdong Women and Children Hospital, No. 521 Xing South Road, Panyu District, Guangzhou 511400, Guangdong, PR China. Electronic address: whycoolkl@163.com.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Affiliated Hospital of Youjiang Medical University, No.18 Zhongshan Road II, Baise 533099, Guangxi, PR China. Electronic address: huangxia722@126.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Huang
authors.affiliation: Affiliated Hospital of Youjiang Medical University, No.18 Zhongshan Road II, Baise 533099, Guangxi, PR China. Electronic address: jiangyujielily@163.com.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Affiliated Hospital of Youjiang Medical University, No.18 Zhongshan Road II, Baise 533099, Guangxi, PR China. Electronic address: gxbsqyq@126.com.
authors.forename: Yueqiu
authors.initials: Y
authors.lastname: Qin
authors.affiliation: People's Hospital of Guangxi Zhuang Autonomous Region, No. 6 Taoyuan Road, Nanning 530021, Guangxi, PR China. Electronic address: icuxiong@sina.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Xiong
authors.affiliation: Minzu Hospital of Guangxi Zhuang Autonomous Region, No. 323 Mingxiu East Road, Nanning 530021, Guangxi, PR China. Electronic address: icuqg@aliyun.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Qin
authors.affiliation: Department of Surgery and Cancer, Imperial College London, Chelsea and Westminster Hospital, London SW10 9NH, UK. Electronic address: s.sooranna@imperial.ac.uk.
authors.forename: Suren R
authors.initials: SR
authors.lastname: Sooranna
authors.affiliation: Affiliated Hospital of Youjiang Medical University, No.18 Zhongshan Road II, Baise 533099, Guangxi, PR China. Electronic address: liaopinhu@163.com.
authors.forename: Liao
authors.initials: L
authors.lastname: Pinhu
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D002681:China; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D016209:Interleukin-8 / Q000097:blood / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D018805:Sepsis / Q000097:blood / Q000235:genetics; D012737:Sex Factors
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8
keywords: IL8; Polymorphism; Sepsis