title: In vivo optical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas.
abstract: Intrinsic optical signal (IOS) imaging promises a noninvasive method for advanced study and diagnosis of eye diseases. Before pursuing clinical applications, it is essential to understand anatomic and physiological sources of retinal IOSs and to establish the relationship between IOS distortions and eye diseases. The purpose of this study was designed to demonstrate the feasibility of <italic<in vivo</italic< IOS imaging of mouse models. A high spatiotemporal resolution spectral domain optical coherence tomography (SD-OCT) was employed for depth-resolved retinal imaging. A custom-designed animal holder equipped with ear bar and bite bar was used to minimize eye movements. Dynamic OCT imaging revealed rapid IOS from the photoreceptor’s outer segment immediately after the stimulation delivery, and slow IOS changes were observed from inner retinal layers. Comparative photoreceptor IOS and electroretinography recordings suggested that the fast photoreceptor IOS may be attributed to the early stage of phototransduction before the hyperpolarization of retinal photoreceptor.
authors.affiliation: University of Illinois at Chicago, Department of Bioengineering, 851 South Morgan Street, Chicago, Illinois 60607, United States.
authors.forename: Benquan
authors.initials: B
authors.lastname: Wang
authors.affiliation: University of Illinois at Chicago, Department of Bioengineering, 851 South Morgan Street, Chicago, Illinois 60607, United States.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Lu
authors.affiliation: University of Illinois at Chicago, Department of Bioengineering, 851 South Morgan Street, Chicago, Illinois 60607, United StatesbUniversity of Illinois at Chicago, Department of Ophthalmology and Visual Sciences, 1855 West Taylor Street, Chicago, Illinois 60612, United States.
authors.forename: Xincheng
authors.initials: X
authors.lastname: Yao