title: Neonatal oxidative stress depends on oxygen blood pressure in umbilical artery.
abstract: With advancing gestation, partial pressure of oxygen (pO2) and pH fall significantly. Hypoxia is a main factor inducing free radical generation and thereby oxidative stress (OS). Placental and fetal tissue response when oxygen becomes restricted is complex and partially known. We tested the hypothesis that changes in umbilical artery and vein blood gas concentrations modulate OS occurrence in the newborn. Seventy umbilical artery and vein plasma samples were collected from healthy term newborns immediately after delivery. F2 Isoprostanes (F2-Isop) were measured in all samples as reliable markers of lipid peroxidation. Significantly lower pCO2 and higher pO2 and pH were found in umbilical vein than in artery, as expected. A positive correlation was detected between pH and pO2 only in umbilical artery (p=0.019). F2-Isop levels were no different between artery and vein in cord blood. Significant correlations were found between F2-Isop and pCO2 (p=0.025) as well as between F2-Isop and pH in umbilical vein (p=0.027). F2-Isop correlated with pCO2 (p=0.007) as well as with pO2 values (p=0.005) in umbilical artery blood. Oxidative stress (OS) in newborns depends on oxygen concentrations in umbilical artery. OS biomarkers significantly correlate with pO2 and in umbilical artery but not in umbilical vein. In normoxic conditions fetal-maternal gas exchanges occurring in placenta re-establish normal higher oxygen levels in umbilical vein than artery, with a normal production of free radicals without any deleterious effects.
authors.affiliation: Department of Molecular and Developmental Medicine, University of Siena, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Proietti
authors.affiliation: Department of Emergency UOC TIN-Neonatology AORN Santobono-Pausilipon Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: De Bernardo
authors.affiliation: Department of Molecular and Developmental Medicine, University of Siena, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Longini
authors.affiliation: Department of Emergency UOC TIN-Neonatology AORN Santobono-Pausilipon Naples, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Sordino
authors.affiliation: Neonatology and Obstetrics Nursing C.G. Ruesch, Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Scaramuzzini
authors.affiliation: Department of Molecular and Developmental Medicine, University of Siena, Italy.
authors.forename: M L
authors.initials: ML
authors.lastname: Tataranno
authors.affiliation: Department of Molecular and Developmental Medicine, University of Siena, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Belvisi
authors.affiliation: Department of Molecular and Developmental Medicine, University of Siena, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bazzini
authors.affiliation: Department of Molecular and Developmental Medicine, University of Siena, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Perrone
authors.affiliation: Department of Molecular and Developmental Medicine, University of Siena, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Buonocore
mesh_terms: D002245:Carbon Dioxide / Q000097:blood; D002585:Cesarean Section; D028441:F2-Isoprostanes / Q000097:blood*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D005609:Free Radicals; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007231:Infant, Newborn / Q000097:blood*; D008297:Male; D018384:Oxidative Stress; D010100:Oxygen / Q000097:blood*; D010102:Oxygen Inhalation Therapy; D010313:Partial Pressure; D011247:Pregnancy; D012016:Reference Values; D014469:Umbilical Arteries; D014471:Umbilical Veins
chemical_list: D028441:F2-Isoprostanes; D005609:Free Radicals; D002245:Carbon Dioxide; D010100:Oxygen