title: TUC338 Overexpression Leads to Enhanced Proliferation and Reduced Apoptosis in Tongue Squamous Cell Carcinoma Cells In Vitro.
abstract: OBJECTIVE
  Long noncoding RNAs are closely related to the development of tumors. In this study, we explored the contribution of the long noncoding RNA TUC338 to cellular processes in tongue squamous cell carcinoma (TSCC).
  
  
  METHODS
  First, we detected TUC338 expression using quantitative reverse transcription-polymerase chain reaction in 25 patients. Then, we transfected a short hairpin RNA to silence TUC338 expression in the CAL-27 and SCC-9 cell lines. Tumor cell growth was determined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, and apoptosis and cell-cycle analyses were performed via flow cytometry.
  
  
  RESULTS
  The results indicated that TUC338 was overexpressed in TSCCs (P < .05). In addition, silencing TUC338 in CAL-27 and SCC-9 cells inhibited cell growth and increased apoptosis significantly in vivo (P < .05).
  
  
  CONCLUSIONS
  Long noncoding RNA TUC338 overexpression leads to enhanced proliferation and reduced apoptosis in TSCC.
authors.affiliation: Professor, Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Ke-Xiong
authors.initials: KX
authors.lastname: Ouyang
authors.affiliation: Resident Doctor, Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zou
authors.affiliation: Professor, Department of Oral and Maxillofacial Surgery, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liang
authors.affiliation: Professor, Department of Stomatology, Guangzhou First People's Hospital, Guangzhou, China.
authors.forename: Zhi-Bao
authors.initials: ZB
authors.lastname: Bai
authors.affiliation: Professor, Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Southern Medical University-Guangdong Provincial Stomatological Hospital, Guangzhou, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: Professor, Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Southern Medical University-Guangdong Provincial Stomatological Hospital, Guangzhou, China. Electronic address: zhaojianjiang1965@hotmail.com.
authors.forename: Jian-Jiang
authors.initials: JJ
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000503:physiopathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D062085:RNA, Long Noncoding / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014062:Tongue Neoplasms / Q000378:metabolism / Q000503:physiopathology*
chemical_list: D062085:RNA, Long Noncoding; C000615821:long non-coding RNA TUC338, human