title: Production of carotenoids and lipids by Rhodococcus opacus PD630 in batch and fed-batch culture.
abstract: Production of carotenoids by Rhodococcus opacus PD630 is reported. A modified mineral salt medium formulated with glycerol as an inexpensive carbon source was used for the fermentation. Ammonium acetate was the nitrogen source. A dry cell mass concentration of nearly 5.4 g/L could be produced in shake flasks with a carotenoid concentration of 0.54 mg/L. In batch culture in a 5 L bioreactor, without pH control, the maximum dry biomass concentration was ~30 % lower than in shake flasks and the carotenoids concentration was 0.09 mg/L. Both the biomass concentration and the carotenoids concentration could be raised using a fed-batch operation with a feed mixture of ammonium acetate and acetic acid. With this strategy, the final biomass concentration was 8.2 g/L and the carotenoids concentration was 0.20 mg/L in a 10-day fermentation. A control of pH proved to be unnecessary for maximizing the production of carotenoids in this fermentation.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand.
authors.forename: Anusith
authors.initials: A
authors.lastname: Thanapimmetha
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand.
authors.forename: Tharatron
authors.initials: T
authors.lastname: Suwaleerat
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand.
authors.forename: Maythee
authors.initials: M
authors.lastname: Saisriyoot
authors.affiliation: School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Chisti
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand. fengpjs@ku.ac.th.
authors.forename: Penjit
authors.initials: P
authors.lastname: Srinophakun
mesh_terms: D018533:Biomass; D019149:Bioreactors; D002338:Carotenoids / Q000096:biosynthesis*; D008055:Lipids / Q000096:biosynthesis*; D012240:Rhodococcus / Q000254:growth & development*
chemical_list: D008055:Lipids; D002338:Carotenoids
keywords: Carotenoids; Fed-batch culture; Fermentation; Rhodococcus opacus PD630