title: Effect of resveratrol on cancer progression through the REG Ⅲ expression pathway in head and neck cancer cells.
abstract: Identification of reliable markers of chemo- and radiosensitivity and the key molecules that enhance the susceptibility of head and neck squamous cell carcinoma (HNSCC) to anticancer treatments is highly desirable. Previously, we have reported that regenerating gene (REG) Ⅲ expression was such a marker associated with an improved survival rate for HNSCC patients. In the present study, we investigated the stimulators for induction of REG Ⅲ expression using REG Ⅲ promoter assay in HNSCC cells transfected with REG Ⅲ promoter vector. We tested inflammatory cytokines, growth factors, polyphenols, PPARγ activator of thiazolidinediones, and histone deacetylase inhibitors, and found that 3,4',5-trihydroxy-trans-stilbene (resveratrol) significantly increased the REG Ⅲ promoter activity and the mRNA levels of REG Ⅲ in HNSCC cells. Moreover, we demonstrated the effect of resveratrol on cancer cell progression, such as cell proliferation, chemo‑ and radiosensitivity and cancer invasion of HNSCC cells. Resveratrol significantly inhibited cell growth, enhanced chemo‑ and radiosensitivity, and blocked cancer invasion of HNSCC cells. These data suggested that resveratrol could inhibit cancer progression through the REG Ⅲ expression pathway in HNSCC cells.
authors.affiliation: Department of Otolaryngology‑Head and Neck Surgery, Nara Medical University, Kashihara, Nara 634‑8522, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Mikami
authors.affiliation: Department of Otolaryngology‑Head and Neck Surgery, Nara Medical University, Kashihara, Nara 634‑8522, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Ota
authors.affiliation: Department of Otolaryngology‑Head and Neck Surgery, Nara Medical University, Kashihara, Nara 634‑8522, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Masui
authors.affiliation: Department of Biochemistry, Nara Medical University, Kashihara, Nara 634‑8522, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Itaya-Hironaka
authors.affiliation: Department of Biochemistry, Nara Medical University, Kashihara, Nara 634‑8522, Japan.
authors.forename: Ryogo
authors.initials: R
authors.lastname: Shobatake
authors.affiliation: Department of Otolaryngology, Nara City Hospital, Nara 630‑8305, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okamoto
authors.affiliation: Department of Biochemistry, Nara Medical University, Kashihara, Nara 634‑8522, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Takasawa
authors.affiliation: Department of Otolaryngology‑Head and Neck Surgery, Nara Medical University, Kashihara, Nara 634‑8522, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kitahara