title: Automated Segmentation of Nuclei in Breast Cancer Histopathology Images.
abstract: The process of Nuclei detection in high-grade breast cancer images is quite challenging in the case of image processing techniques due to certain heterogeneous characteristics of cancer nuclei such as enlarged and irregularly shaped nuclei, highly coarse chromatin marginalized to the nuclei periphery and visible nucleoli. Recent reviews state that existing techniques show appreciable segmentation accuracy on breast histopathology images whose nuclei are dispersed and regular in texture and shape; however, typical cancer nuclei are often clustered and have irregular texture and shape properties. This paper proposes a novel segmentation algorithm for detecting individual nuclei from Hematoxylin and Eosin (H&E) stained breast histopathology images. This detection framework estimates a nuclei saliency map using tensor voting followed by boundary extraction of the nuclei on the saliency map using a Loopy Back Propagation (LBP) algorithm on a Markov Random Field (MRF). The method was tested on both whole-slide images and frames of breast cancer histopathology images. Experimental results demonstrate high segmentation performance with efficient precision, recall and dice-coefficient rates, upon testing high-grade breast cancer images containing several thousand nuclei. In addition to the optimal performance on the highly complex images presented in this paper, this method also gave appreciable results in comparison with two recently published methods-Wienert et al. (2012) and Veta et al. (2013), which were tested using their own datasets.
authors.affiliation: Department of Mathematics, Madras Christian College, Chennai, India.
authors.forename: Maqlin
authors.initials: M
authors.lastname: Paramanandam
authors.affiliation: School of Mechanical and Materials Engineering, University College Dublin, Ireland.
authors.forename: Michael
authors.initials: M
authors.lastname: O'Byrne
authors.affiliation: Department of Civil, Structural and Environmental Engineering, Trinity College Dublin, Ireland.
authors.forename: Bidisha
authors.initials: B
authors.lastname: Ghosh
authors.affiliation: Department of Transfusion Medicine & Immunohematology, Christian Medical College, Vellore, India.
authors.forename: Joy John
authors.initials: JJ
authors.lastname: Mammen
authors.affiliation: Department of Pathology, Christian Medical College, Vellore, India.
authors.forename: Marie Therese
authors.initials: MT
authors.lastname: Manipadam
authors.affiliation: Department of Mathematics, Madras Christian College, Chennai, India.
authors.forename: Robinson
authors.initials: R
authors.lastname: Thamburaj
authors.affiliation: School of Mechanical and Materials Engineering, University College Dublin, Ireland.
authors.forename: Vikram
authors.initials: V
authors.lastname: Pakrashi
mesh_terms: D000465:Algorithms; D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000473:pathology*; D002467:Cell Nucleus / Q000473:pathology*; D005260:Female; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*