title: Involvement of PGRP-SC2 from Artemia sinica in the innate immune response against bacteria and expression pattern at different developmental stages.
abstract: Peptidoglycan-recognition protein-SC2 precursor-like protein (PGRP-SC2) is a vital protein in innate immunity with a vita role in response to bacteria challenge in invertebrates. Here, a 678-bp full-length cDNA of pgrp-sc2 from A. sinica was obtained containing a 558-bp open reading frame encoding 185 amino acids with a calculated molecular mass of 19.6 kDa. The predicted protein contains a PGRP and an Amidase2 domain, indicating that PGRP-SC2 is a PGRP family member and has N-acetylmuramoyl-l-alanine amidase activity. The expression and localization of pgrp-sc2/PGRP-SC2 in A.sinica during embryonic development and bacterial challenge were determined by qPCR, WB and ISH. During different A. sinica embryonic development stages, the expression level of pgrp-sc2/PGRP-SC2 was most highly expressed at 0 and 5 h and after challenge by Gram-positive bacteria, it increased with increasing bacterial concentrations, indicating that it plays a vital role in A. sinica early embryonic development and innate immunity.
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Mengchen
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yao
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian 116044, China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian 116044, China. Electronic address: zouxiangyang@126.com.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zou
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China. Electronic address: houlin@lnnu.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
mesh_terms: D000818:Animals; D001156:Artemia / Q000276:immunology*; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics; D002478:Cells, Cultured; D003001:Cloning, Molecular; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D007113:Immunity, Innate; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D016415:Sequence Alignment; D059467:Transcriptome; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology*
chemical_list: D060829:Arthropod Proteins; D002352:Carrier Proteins; C118897:peptidoglycan recognition protein; D009238:N-Acetylmuramoyl-L-alanine Amidase
keywords: Artemia sinica; Bacterial infection; Development; Expression pattern; pgrp-sc2