title: Effects of Illumination Pattern during Cultivation of Fruiting Body and Bioactive Compound Production by the Caterpillar Medicinal Mushroom, Cordyceps militaris (Ascomycetes).
abstract: We investigated the effects of light intensity in the 3 cultivation stages separately-the mycelium colonization stage, the primordial initiation stage, and the fruiting stage (in order)-on fruiting body and bioactive compound production by Cordyceps militaris. In the mycelium colonization stage, rice substrates were incubated in a spawn running room at 23°C. During the primordial initiation stage, C. militaris was grown at 18°C and illuminated 12 hours/day. In the fruiting stage the temperature was 23°C, with illumination provided 12 hours/day. The highest fruiting body yield and biological efficiency were 4.06 g dry weight/bottle and 86.83%, respectively, under 1750 ± 250 lux during the second and third stages. The cordycepin content was highest during the second and third stages under 1250 ± 250 lux. The mannitol and polysaccharide contents were highest under 1250 ± 250 and 1750 ± 250 lux during the primordial initiation stage and the fruiting stage, respectively. Thus, with controlled lighting, C. militaris can be cultivated in rice-water medium to increase fruiting body yield and bioactive compound production.
authors.affiliation: Department of Culinary Arts, Chung Chou University of Science and Technology, Changhua, Taiwan, R.O.C.
authors.forename: Chiu-Yeh
authors.initials: CY
authors.lastname: Wu
authors.affiliation: Department of Bioresources, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.).
authors.forename: Zeng-Chin
authors.initials: ZC
authors.lastname: Liang
authors.affiliation: Department of Health Food, Chung Chou University of Science and Technology, Changhua, Taiwan, R.O.C.
authors.forename: Chin-Yin
authors.initials: CY
authors.lastname: Tseng
authors.affiliation: Department of Nutrition and Dietetics, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Hu
mesh_terms: D000383:Agriculture; D032661:Cordyceps / Q000378:metabolism* / Q000528:radiation effects*; D048690:Fruiting Bodies, Fungal / Q000502:physiology* / Q000528:radiation effects*; D008027:Light