title: Differential Expression of MicroRNAs in Endarterectomy Specimens Taken from Patients with Asymptomatic and Symptomatic Carotid Plaques.
abstract: OBJECTIVE
  Stroke and transient ischemic attacks are considered as clinical manifestations of atherosclerotic disease due to on-going vascular inflammation and finally atherothrombosis of the carotid arteries. MicroRNAs (miRNA/miR) are known to be involved in vascular inflammation and plaque destabilization. The aim of this study was to analyze the expression profile of selected miRNAs in endarterectomy specimen from carotid arteries that were taken from patients with asymptomatic and symptomatic atherosclerotic plaques.
  
  
  RESULTS
  11 miRNAs were selected and their expression was analyzed using real-time RT-PCR. Therefore, samples were divided into three different groups. On the one hand we investigated the expression patterns from patients in asymptomatic (n = 14) and symptomatic (n = 10) plaques; on the other hand we took samples from normal configurated internal mammary arteries (n = 15). Out of these 11 targets we identified some miRNAs, which were up- or down-regulated in either one of the two groups. Interestingly, the expression of two miRNAs was significantly different between asymptomatic and symptomatic samples, namely miR-21 (P<0.01) and miR-143 (P<0.05).
  
  
  CONCLUSIONS
  In the present study, we identified miRNA subtypes which showed different expression in endarterectomy specimen from patients with asymptomatic and symptomatic plaques, suggesting that these miRNAs correlated with advanced vascular inflammation and plaque stability. They may represent new therapeutic targets for vascular proliferative diseases such as atherosclerosis.
authors.affiliation: Internal Medicine/Cardiology and Angiology, University Hospital of Giessen and Marburg, Location Marburg, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Markus
authors.affiliation: Internal Medicine/Cardiology and Angiology, University Hospital of Giessen and Marburg, Location Marburg, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: Grote
authors.affiliation: Internal Medicine I/Cardiology and Angiology, University Hospital of Giessen and Marburg, Location Giessen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Worsch
authors.affiliation: Internal Medicine I/Cardiology and Angiology, University Hospital of Giessen and Marburg, Location Giessen, Germany.
authors.forename: Behnoush
authors.initials: B
authors.lastname: Parviz
authors.affiliation: Department of Cardiovascular Surgery, University Hospital of Giessen and Marburg, Location Giessen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Boening
authors.affiliation: Internal Medicine/Cardiology and Angiology, University Hospital of Giessen and Marburg, Location Marburg, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Schieffer
authors.affiliation: Internal Medicine/Cardiology and Angiology, University Hospital of Giessen and Marburg, Location Marburg, Germany.|Internal Medicine I/Cardiology and Angiology, University Hospital of Giessen and Marburg, Location Giessen, Germany.
authors.forename: Mariana S
authors.initials: MS
authors.lastname: Parahuleva
mesh_terms: D000368:Aged; D002339:Carotid Arteries / Q000378:metabolism*; D016894:Endarterectomy, Carotid; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*
chemical_list: D035683:MicroRNAs