title: Study of apoptosis-related interactions in colorectal cancer.
abstract: Abnormalities in apoptotic functions contribute to the pathogenesis of colorectal cancer. In this study, molecular interactions behind the apoptotic regulation have been explored. For this purpose, enrichment analysis was performed considering microRNAs (miRNAs) that putatively target TP53 and altered during colon cancer. This revealed gene associated with both TP53 and miRNAs. Further analysis showed that a significant molecular interaction between the shortlisted candidates (TP53, miR-143, KRAS, BCL2, and PLK1) exists. Mutation study was conducted to confirm the clinical relevance of candidates. It showed that the mutation extent does not significantly alter survival in patients thus making these candidates suitable as drug targets. Overall, we showed the importance of interactions between TP53, miR-143, KRAS, BCL2, and PLK1 with respect to colorectal cancer using bioinformatics approach.
authors.affiliation: School of Studies in Zoology and Biotechnology, Vikram University, Ujjain, MP, India. himanshu.harora@gmail.com.
authors.forename: Himanshu
authors.identifier: http://orcid.org/0000-0003-3143-6387
authors.initials: H
authors.lastname: Arora
authors.affiliation: Genome Biology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
authors.forename: Rehana
authors.initials: R
authors.lastname: Qureshi
authors.affiliation: Genome Biology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
authors.forename: M A
authors.initials: MA
authors.lastname: Rizvi
authors.affiliation: School of Studies in Zoology and Biotechnology, Vikram University, Ujjain, MP, India.
authors.forename: Sharad
authors.initials: S
authors.lastname: Shrivastava
authors.affiliation: School of Studies in Zoology and Biotechnology, Vikram University, Ujjain, MP, India.
authors.forename: Mordhwaj S
authors.initials: MS
authors.lastname: Parihar
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics*; D049109:Cell Proliferation; D003106:Colon / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D035683:MicroRNAs; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016283:Proto-Oncogene Proteins p21(ras); D016159:Tumor Suppressor Protein p53; D000097869:Polo-Like Kinase 1; C000595707:BCL2 protein, human; C542359:MIRN143 microRNA, human; C495901:TP53 protein, human
keywords: Apoptosis; Colorectal cancer; TP53; miRNA