title: Gene Expression Patterns Are Altered in Athymic Mice and Metabolic Syndrome Factors Are Reduced in C57BL/6J Mice Fed High-Fat Diets Supplemented with Soy Isoflavones.
abstract: Soy isoflavones exert beneficial health effects; however, their potential to ameliorate conditions associated with the metabolic syndrome (MetS) has not been studied in detail. In vitro and in vivo models were used to determine the effect of isoflavones on lipid metabolism, inflammation, and oxidative stress. In nude mice, consumption of Novasoy (NS) increased cholesterol and lipid metabolism gene expression, including Scd-1 (27.7-fold), Cyp4a14 (35.2-fold), and Cyp4a10 (9.5-fold), and reduced anti-inflammatory genes, including Cebpd (16.4-fold). A high-fat (HF) diet containing 0.4% (w/w) NS for 10 weeks significantly reduced percent weight gain (74.6 ± 2.5 vs 68.6 ± 3.5%) and hepatic lipid accumulation (20 ± 1.2 vs 27 ± 1.5%), compared to HF alone (p < 0.05) in C57BL/6J mice. NS also increased lipid oxidation and antioxidant gene expression while decreasing inflammatory cytokines. In vitro analysis in HepG2 cells revealed that genistein dose-dependently decreases oleic acid-induced lipid accumulation. Soy isoflavones may ameliorate symptoms associated with MetS via anti-inflammatory, antioxidant, and hypolipidemic modulation.
authors.affiliation: Food Science and Technology, Oregon State University , Corvallis, Oregon 97330, United States.
authors.forename: Ting
authors.initials: T
authors.lastname: Luo
authors.affiliation: Food Science and Technology, Oregon State University , Corvallis, Oregon 97330, United States.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Snyder
authors.affiliation: Food Science and Technology, Oregon State University , Corvallis, Oregon 97330, United States.
authors.forename: Bingxin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Dietetics and Nutrition, Kansas University Medical Center , Kansas City, Kansas 66160, United States.
authors.forename: Debra K
authors.initials: DK
authors.lastname: Sullivan
authors.affiliation: Dietetics and Nutrition, Kansas University Medical Center , Kansas City, Kansas 66160, United States.
authors.forename: Jill
authors.initials: J
authors.lastname: Hamilton-Reeves
authors.affiliation: Baylor College of Medicine , Houston, Texas 77030, United States.
authors.forename: Gregory
authors.initials: G
authors.lastname: Guthrie
authors.affiliation: Agriculture, Nutrition and Veterinary Sciences, University of Nevada , Reno, Nevada 89557, United States.
authors.forename: Marie-Louise
authors.initials: ML
authors.lastname: Ricketts
authors.affiliation: Pharmacology, University of Florida College of Medicine , Gainesville, Florida 32610, United States.
authors.forename: Kathleen T
authors.initials: KT
authors.lastname: Shiverick
authors.affiliation: Food Science and Technology, Oregon State University , Corvallis, Oregon 97330, United States.
authors.forename: Neil
authors.initials: N
authors.lastname: Shay
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D019587:Dietary Supplements; D005786:Gene Expression Regulation / Q000187:drug effects*; D019833:Genistein / Q000494:pharmacology; D056945:Hep G2 Cells / Q000187:drug effects; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects* / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D024821:Metabolic Syndrome / Q000178:diet therapy* / Q000209:etiology; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D019301:Oleic Acid / Q000494:pharmacology; D013025:Glycine max / Q000737:chemistry
chemical_list: D007529:Isoflavones; D019301:Oleic Acid; D019833:Genistein
keywords: genistein; inflammation; isoflavones; lipid metabolism; metabolic syndrome; oxidation