title: A Characeae Cells Plasma Membrane as a Model for Selection of Bioactive Compounds and Drugs: Interaction of HAMLET-Like Complexes with Ion Channels of Chara corallina Cells Plasmalemma.
abstract: Interaction of a HAMLET-like La-OA cytotoxic complex (human α-lactalbumin-oleic acid) and its constituents with the excitable plasmalemma of giant Chara corallina cells was investigated. The voltage-clamp technique was used to study Ca2+and Cl-transient currents in the plasmalemma of intact cells. The action of the complex and OA on the target cell membrane has a dose-dependent character. It was found that the La-OA complex has an inhibiting effect on Ca2+current across the plasmalemma, while α-lactalbumin alone does not affect the electrophysiological characteristics of the cellular membrane. However, oleic acid blocks Ca2+current across the plasmalemma. This is accompanied by the induction of a non-selective conductivity in the cellular membrane, a decrease in the resting potential and plasma membrane resistance of algal cells. We propose that the cytotoxicity of La-OA and other HAMLET-like complexes is determined by oleic acid acting as a blocker of potential-dependent Ca2+channels in the plasma membrane of target cells. The presented results show that the study model of green algae C. corallina cells plasmalemma is a convenient tool for the investigation of ion channels in many animal cells.
authors.affiliation: Institute of Cell Biophysics of the RAS, Institutskaya 3, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Anatoly
authors.initials: A
authors.lastname: Kataev
authors.affiliation: Institute of Theoretical and Experimental Biophysics of the RAS, Institutskaya 3, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Olga
authors.initials: O
authors.lastname: Zherelova
authors.affiliation: Institute for Biological Instrumentation of the RAS, Institutskaya 7, Pushchino, Moscow Region, 142290, Russia. valerygrishchenko@mail.ru.
authors.forename: Valery
authors.initials: V
authors.lastname: Grishchenko
mesh_terms: D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism*; D044522:Characeae / Q000166:cytology* / Q000378:metabolism*; D055808:Drug Discovery / Q000379:methods; D055724:Electrophysiological Phenomena / Q000187:drug effects; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects; D007473:Ion Channels / Q000378:metabolism; D007768:Lactalbumin / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D019301:Oleic Acid / Q000494:pharmacology; D009829:Oleic Acids / Q000378:metabolism; D000071037:Place Cells / Q000378:metabolism*
chemical_list: C506422:HAMLET complex, human; D007473:Ion Channels; D009829:Oleic Acids; D019301:Oleic Acid; D007768:Lactalbumin
keywords: Characeae cells; HAMLET; Ionic channels; La–OA complex; Oleic acid; Plasmalemma; α-Lactalbumin