title: Proteomic Investigation of Ram Spermatozoa and the Proteins Conferred by Seminal Plasma.
abstract: Sperm proteomes have emerged for several species; however, the extent of species similarity is unknown. Sheep are an important agricultural species for which a comprehensive sperm proteome has not been produced. In addition, potential proteomic factors from seminal plasma that may contribute to improved fertility after cervical insemination are yet to be explored. Here we use liquid chromatography-tandem mass spectrometry to investigate the proteome of ejaculated ram spermatozoa, with quantitative comparison to epididymal spermatozoa. We also present a comparison to published proteomes of five other species. We identified 685 proteins in ejaculated ram spermatozoa, with the most abundant proteins involved in metabolic pathways. Only 5% of ram sperm proteins were not detected in other species, which suggest highly conserved structures and pathways. Of the proteins present in both epididymal and ejaculated ram spermatozoa, 7% were more abundant in ejaculated spermatozoa. Only two membrane-bound proteins were detected solely in ejaculated sperm lysates: liver enriched gene 1 (LEG1/C6orf58) and epidermal growth factor-like repeats and discoidin I-like domains 3 (EDIL3). This is the first evidence that despite its relatively complex proteomic composition, seminal plasma exposure leads to few novel proteins binding tightly to the ram sperm plasma membrane.
authors.affiliation: Faculty of Veterinary Science, School of Life and Environmental Sciences, The University of Sydney , Sydney, New South Wales 2006, Australia.
authors.forename: Taylor
authors.initials: T
authors.lastname: Pini
authors.affiliation: Faculty of Veterinary Science, School of Life and Environmental Sciences, The University of Sydney , Sydney, New South Wales 2006, Australia.
authors.forename: Tamara
authors.initials: T
authors.lastname: Leahy
authors.affiliation: PRC, INRA, CNRS, IFCE, Université de Tours , 37380 Nouzilly, France.
authors.forename: Clement
authors.initials: C
authors.lastname: Soleilhavoup
authors.affiliation: PRC, INRA, CNRS, IFCE, Université de Tours , 37380 Nouzilly, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Tsikis
authors.affiliation: PRC, INRA, CNRS, IFCE, Université de Tours , 37380 Nouzilly, France.
authors.forename: Valerie
authors.initials: V
authors.lastname: Labas
authors.affiliation: PRC, INRA, CNRS, IFCE, Université de Tours , 37380 Nouzilly, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Combes-Soia
authors.affiliation: PRC, INRA, CNRS, IFCE, Université de Tours , 37380 Nouzilly, France.
authors.forename: Gregoire
authors.initials: G
authors.lastname: Harichaux
authors.affiliation: Faculty of Veterinary Science, School of Life and Environmental Sciences, The University of Sydney , Sydney, New South Wales 2006, Australia.
authors.forename: Jessica P
authors.initials: JP
authors.lastname: Rickard
authors.affiliation: PRC, INRA, CNRS, IFCE, Université de Tours , 37380 Nouzilly, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Druart
authors.affiliation: Faculty of Veterinary Science, School of Life and Environmental Sciences, The University of Sydney , Sydney, New South Wales 2006, Australia.
authors.forename: Simon P
authors.initials: SP
authors.lastname: de Graaf
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D002853:Chromatography, Liquid; D005298:Fertility; D008297:Male; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D029589:Seminal Plasma Proteins / Q000032:analysis*; D012756:Sheep; D013094:Spermatozoa / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D011506:Proteins; D029589:Seminal Plasma Proteins
keywords: MS; ovine; ram spermatozoa; seminal plasma; species comparison; sperm proteome