title: Chromatin Immunoprecipitation.
abstract: Chromatin immunoprecipitation (ChIP) is a valuable method to investigate protein-DNA interactions in vivo. Since its discovery it has been indispensable to identify binding sites and patterns of a variety of DNA-interacting proteins, such as transcription factors and regulators, modified histones, and epigenetic modifiers. The Polycomb repressors were the first proteins that have been mapped using this technique, which provided the mechanistic basis for the understanding of their biological function. Cross-linked (XChIP) or native (NChIP) chromatin from tissues or cultured cells is fragmented and the protein of interest is immunoprecipitated using a specific antibody. The co-precipitated DNA is then purified and subjected to analysis by region-specific PCR, DNA microarray (ChIP-on-chip), or next-generation sequencing (ChIP-seq). The assay can therefore produce information about the localization of the analyzed protein at specific candidate loci or throughout the entire genome. In this chapter, we provide a detailed protocol of the basic standard ChIP assay and some remarks about variations.
authors.affiliation: Division of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, Im Neuenheimer Feld 580, 69120, Heidelberg, Germany. l.wiehle@dkfz.de.
authors.forename: Laura
authors.initials: L
authors.lastname: Wiehle
authors.affiliation: Division of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, Im Neuenheimer Feld 580, 69120, Heidelberg, Germany.
authors.forename: Achim
authors.initials: A
authors.lastname: Breiling
mesh_terms: D001665:Binding Sites; D002843:Chromatin / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification*; D059014:High-Throughput Nucleotide Sequencing; D008967:Molecular Biology / Q000379:methods*; D011485:Protein Binding
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D004247:DNA
keywords: ChIP; Chromatin; Cross-linking; DNA-protein interactions; Immunoprecipitation; Repressive protein complexes