title: Electroactive and biocompatible functionalization of graphene for the development of biosensing platforms.
abstract: Design and synthesis of low-cost, highly stable, electroactive and biocompatible material is one of the key steps for the advancement of electrochemical biosensing systems. To this end, we have explored a facile way for the successful synthesis of redox active and bioengineering of reduced graphene oxide (RGO) for the development of versatile biosensing platform. A highly branched polymer (PEI) is used for reduction and simultaneous derivation of graphene oxide (GO) to form a biocompatible polymeric matrix on RGO nanosheet. Ferrocene redox moieties are then wired onto RGO nanosheets through the polymer matrix. The as-prepared functional composite is electrochemically active and enables to accommodate enzymes stably. For proof-of-concept studies, two crucial redox enzymes for biosensors (i.e. cholesterol oxidase and glucose oxidase) are targeted. The enzyme integrated and RGO supported biosensing hybrid systems show high stability, excellent selectivity, good reproducibility and fast sensing response. As measured, the detection limit of the biosensors for glucose and cholesterol is 5µM and 0.5µM (S/N=3), respectively. The linear response range of the biosensor is from 0.1 to 15.5mM for glucose and from 2.5 to 25µM for cholesterol. Furthermore, this biosensing platform shows good anti-interference ability and reasonable stability. The nanohybrid biosensing materials can be combined with screen-printed electrodes, which are successfully used for measuring the glucose and cholesterol level of real human serum samples.
authors.affiliation: Department of Chemistry, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.
authors.forename: Arnab
authors.initials: A
authors.lastname: Halder
authors.affiliation: Department of Chemistry, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.
authors.forename: Minwei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark. Electronic address: cq@kemi.dtu.dk.
authors.forename: Qijin
authors.initials: Q
authors.lastname: Chi
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D002784:Cholesterol / Q000097:blood*; D002789:Cholesterol Oxidase / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D001672:Biocompatible Materials; D001786:Blood Glucose; D004800:Enzymes, Immobilized; D010087:Oxides; D006108:Graphite; D002784:Cholesterol; D005949:Glucose Oxidase; D002789:Cholesterol Oxidase
keywords: Cholesterol oxidase; Electroactive functionalization; Electrochemical biosensor; Glucose oxidase; Human serum sample; Reduced graphene oxide