title: Infrared Methods for Assessment of the Activity of Natural Enamel Caries Lesions.
abstract: New diagnostic methods are needed for the accurate assessment of caries lesion activity to establish the need for surgical treatment. Detection of the highly mineralized surface layer that forms near the surface of the lesions as a result of remineralization is important for diagnosis of the lesion activity. Previous studies have demonstrated that novel imaging methods can be used to detect remineralization of artificial enamel caries lesions. In this paper, the activity of natural enamel caries lesions was assessed in-vitro via detection of the surface layer with PS-OCT and dehydration rate measurements with NIR reflectance and thermal imaging modalities. An automated approach for detecting the surface layer with PS-OCT yielded high sensitivity (= 0.79) and high specificity (= 0.93) with moderate correlation (R2= 0.5920) with histology. Significant differences in dehydration rate measurements were found between the active and the arrested lesions using both the NIR reflectance and thermal imaging modalities. These results demonstrate that these novel imaging methods are ideally suited for nondestructive, noninvasive and quantitative measurement of lesion activity during a single clinical examination in real-time.
authors.affiliation: Division of Biomaterials and Bioengineering, Department of Preventative and Restorative Dental Sciences, San Francisco School of Dentistry, University of California, San Francisco, CA 94143-0758 USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Lee
authors.affiliation: Division of Biomaterials and Bioengineering, Department of Preventative and Restorative Dental Sciences, San Francisco School of Dentistry, University of California, San Francisco, CA 94143-0758 USA.
authors.forename: Michal
authors.initials: M
authors.lastname: Staninec
authors.affiliation: Division of Biomaterials and Bioengineering, Department of Preventative and Restorative Dental Sciences, San Francisco School of Dentistry, University of California, San Francisco, CA 94143-0758 USA.
authors.forename: Oanh
authors.initials: O
authors.lastname: Le
authors.affiliation: Division of Biomaterials and Bioengineering, Department of Preventative and Restorative Dental Sciences, San Francisco School of Dentistry, University of California, San Francisco, CA 94143-0758 USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Fried
keywords: Dental Caries; Enamel; Near-infrared imaging; Polarization-sensitive optical coherence tomography (PS-OCT); Remineralization