title: N-Acetyl-D-Glucosamine Kinase Interacts with Dynein-Lis1-NudE1 Complex and Regulates Cell Division.
abstract: N-acetyl-D-glucosamine kinase (GlcNAc kinase or NAGK) primarily catalyzes phosphoryl transfer to GlcNAc during amino sugar metabolism. Recently, it was shown NAGK interacts with dynein light chain roadblock type 1 (DYNLRB1) and upregulates axo-dendritic growth, which is an enzyme activity-independent, non-canonical structural role. The authors examined the distributions of NAGK and NAGK-dynein complexes during the cell cycle in HEK293T cells. NAGK was expressed throughout different stages of cell division and immunocytochemistry (ICC) showed NAGK was localized at nuclear envelope, spindle microtubules (MTs), and kinetochores (KTs). A proximity ligation assay (PLA) for NAGK and DYNLRB1 revealed NAGK-dynein complex on nuclear envelopes in prophase cells and on chromosomes in metaphase cells. NAGK-DYNLRB1 PLA followed by Lis1/NudE1 immunostaining showed NAGK-dynein complexes were colocalized with Lis1 and NudE1 signals, and PLA for NAGK-Lis1 showed similar signal patterns, suggesting a functional link between NAGK and dynein-Lis1 complex. Subsequently, NAGK-dynein complexes were found in KTs and on nuclear membranes where KTs were marked with CENP-B ICC and nuclear membrane with lamin ICC. Furthermore, knockdown of NAGK by small hairpin (sh) RNA was found to delay cell division. These results indicate that the NAGK-dynein interaction with the involvements of Lis1 and NudE1 plays an important role in prophase nuclear envelope breakdown (NEB) and metaphase MT-KT attachment during eukaryotic cell division.
authors.affiliation: Department of Anatomy, Dongguk Medical Institute, Dongguk University Graduate School of Medicine, Gyeongju 38066, Korea.
authors.forename: Syeda Ridita
authors.initials: SR
authors.lastname: Sharif
authors.affiliation: Department of Anatomy, Dongguk Medical Institute, Dongguk University Graduate School of Medicine, Gyeongju 38066, Korea.
authors.forename: Ariful
authors.initials: A
authors.lastname: Islam
authors.affiliation: Department of Anatomy, Dongguk Medical Institute, Dongguk University Graduate School of Medicine, Gyeongju 38066, Korea.|Section of Neuroscience, Dongguk Medical Institute, Dongguk University Graduate School of Medicine, Gyeongju 38066, Korea.
authors.forename: Il Soo
authors.initials: IS
authors.lastname: Moon
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D002877:Chromosomes, Human; D056445:Cytoplasmic Dyneins / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D018386:Kinetochores / Q000502:physiology; D008677:Metaphase / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
chemical_list: C504797:DYNLRB1 protein, human; D008869:Microtubule-Associated Proteins; C525094:Nde1 protein, human; D017853:Phosphotransferases (Alcohol Group Acceptor); C018716:N-acetylglucosamine kinase; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; C084102:PAFAH1B1 protein, human; D056445:Cytoplasmic Dyneins; D004398:Dyneins
keywords: Lis1; NAGK; NudE1; cell division; dynein; kinetochore; microtubule; nuclear envelope