title: A novel family of plant nuclear envelope-associated proteins.
abstract: This paper describes the characterisation of a new family of higher plant nuclear envelope-associated proteins (NEAPs) that interact with other proteins of the nuclear envelope. In the model plant Arabidopsis thaliana, the family consists of three genes expressed ubiquitously (AtNEAP1-3) and a pseudogene (AtNEAP4). NEAPs consist of extensive coiled-coil domains, followed by a nuclear localisation signal and a C-terminal predicted transmembrane domain. Domain deletion mutants confirm the presence of a functional nuclear localisation signal and transmembrane domain. AtNEAP proteins localise to the nuclear periphery as part of stable protein complexes, are able to form homo- and heteromers, and interact with the SUN domain proteins AtSUN1 and AtSUN2, involved in the linker of nucleoskeleton and cytoskeleton (LINC) complex. An A. thaliana cDNA library screen identified a putative transcription factor called AtbZIP18 as a novel interactor of AtNEAP1, which suggest a connection between NEAP and chromatin. An Atneap1 Atneap3 double-knockout mutant showed reduced root growth, and altered nuclear morphology and chromatin structure. Thus AtNEAPs are suggested as inner nuclear membrane-anchored coiled-coil proteins with roles in maintaining nuclear morphology and chromatin structure.
authors.affiliation: Department of Biological and Medical Sciences, Faculty of Health and Life Sciences, Oxford Brookes University, Oxford, OX3 0BP, UK UMR CNRS 6293 INSERM U 1103 Clermont Université, GReD, Avenue des Landais 63171 Aubière Cedex, France.
authors.forename: Vidya
authors.initials: V
authors.lastname: Pawar
authors.affiliation: Department of Biological and Medical Sciences, Faculty of Health and Life Sciences, Oxford Brookes University, Oxford, OX3 0BP, UK UMR CNRS 6293 INSERM U 1103 Clermont Université, GReD, Avenue des Landais 63171 Aubière Cedex, France.
authors.forename: Axel
authors.initials: A
authors.lastname: Poulet
authors.affiliation: Department of Biological and Medical Sciences, Faculty of Health and Life Sciences, Oxford Brookes University, Oxford, OX3 0BP, UK UMR CNRS 6293 INSERM U 1103 Clermont Université, GReD, Avenue des Landais 63171 Aubière Cedex, France.
authors.forename: Gwénaëlle
authors.initials: G
authors.lastname: Détourné
authors.affiliation: UMR CNRS 6293 INSERM U 1103 Clermont Université, GReD, Avenue des Landais 63171 Aubière Cedex, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Tatout
authors.affiliation: UMR CNRS 6293 INSERM U 1103 Clermont Université, GReD, Avenue des Landais 63171 Aubière Cedex, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Vanrobays
authors.affiliation: Department of Biological and Medical Sciences, Faculty of Health and Life Sciences, Oxford Brookes University, Oxford, OX3 0BP, UK.
authors.forename: David E
authors.initials: DE
authors.lastname: Evans
authors.affiliation: Department of Biological and Medical Sciences, Faculty of Health and Life Sciences, Oxford Brookes University, Oxford, OX3 0BP, UK kgraumann@brookes.ac.uk.
authors.forename: Katja
authors.initials: K
authors.lastname: Graumann
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D015153:Blotting, Western; D002843:Chromatin / Q000378:metabolism; D003001:Cloning, Molecular; D003599:Cytoskeleton / Q000378:metabolism; D017343:Genes, Plant; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D018613:Microscopy, Confocal; D009685:Nuclear Envelope / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D002843:Chromatin; D008565:Membrane Proteins
keywords: Arabidopsis thaliana; LINC complex; chromatin; higher plant; inner nuclear membrane; nuclear envelope; nuclear lamina; nucleoskeleton; nucleus.