title: The Clinical and Medicolegal Analysis of Electrical Shocked Rats: Based on the Serological and Histological Methods.
abstract: This research was aimed at discovering the serological and histological changes in cardiac and hepatic tissue after electric shock. The CK-MB, ALT, and AMS indexes were tested with serological methods. Moreover, the Bcl-2, Bax, and Hsp-60 expression levels were carefully measured. An electrical injury model was established by giving rats electric shocks at 110 V with alternating electric current. Blood samples from the rats were analyzed for the biochemical indexes. The degrees of pathological changes in the heart and liver were evaluated using IHC staining for Bcl-2, Bax, and Hsp-60. The levels of CK-MB in the electrical injury group rapidly peaked at 0.5 hours after the electric shock. Additionally, the levels of Bcl-2, Bax, and Hsp-60 in the cardiac and hepatic tissues changed regularly after the electrical injury and exhibited apparent differences from the levels in the control group. CK-MB, ALT, and AMS were altered regularly after electric shock, and these results provide significant information for clinical and medicolegal practice. This research has shed light on the assessment of electrical injury without obvious electrical burns. Furthermore, the findings obtained for Bcl-2/Bax and Hsp-60 can also facilitate pathological diagnosis and the identification of antemortem and postmortem electrical injury.
authors.affiliation: Shaanxi Provincial People's Hospital, The Third Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China; School of Forensic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an, China.
authors.forename: Huitong
authors.identifier: 0000-0003-4585-7932
authors.initials: H
authors.lastname: Liu
authors.affiliation: Shaanxi Provincial People's Hospital, The Third Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China; School of Forensic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an, China.
authors.forename: Qiaofeng
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Shaanxi Provincial People's Hospital, The Third Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China; School of Forensic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an, China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: School of Forensic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an, China; Department of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Shaanxi Provincial People's Hospital, The Third Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China; School of Forensic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an, China.
authors.forename: Suzhen
authors.initials: S
authors.lastname: Ding
authors.affiliation: School of Forensic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an, China.
authors.forename: Zhenyuan
authors.identifier: 0000-0003-3561-6414
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D004597:Electroshock; D006360:Heat-Shock Proteins / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism; D007603:Jurisprudence; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D015415:Biomarkers; D006360:Heat-Shock Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein