title: Preliminary characterizations, antioxidant and hepatoprotective activity of polysaccharide from Cistanche deserticola.
abstract: To research the preliminary characterizations, antioxidant and hepatoprotective activity of polysaccharides from Cistanche deserticola (CDPs), three polysaccharide fractions, CDP-A, CDP-B and CDP-C, were obtained by successively membrane filtration (microfiltration, ultrafiltration and nanofiltration). Molecular weights, monosaccharide compositions, purities and IR spectra of the three fractions were analyzed. Results showed that CDP-C contained higher proportion of galacturonic acid (GalUA) than CDP-B and CDP-A. Antioxidant activities were also analyzed and the results revealed that CDP-C possessed the highest activity. Thus, hepatoprotective activity of CDP-C was studied further. In vitro research, CDP-C promoted viability of HepG2 cells. In vivo research, CDP-C ameliorated the alterations induced by alcohol, including serological indexes (alanine transaminase, acid phosphatase, γ-glutamyl transpeptidase and triglyceride) and hepatic indicators (superoxide dismutase, malondialdehyde, glutathione S-transferase and triglyceride) in model animals. The prominent microvesicular steatosis and mild necrosis in hepatic histopathology of model animals were also attenuated by CDP-C administration. These findings indicated that CDP-C possessed hepatoprotective activity against chronic hepatic injury induced by alcohol. The underlying mechanism might be that CDP-C can reduce the contents of MDA and TG, and modulate the activities of the relative enzyme. This property might associate with GalUA in CDP-C.
authors.affiliation: Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China; University of Chinese Academy of Sciences, Beijing,100049, PR China.
authors.forename: Yuanheng
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Cao
authors.affiliation: Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China.
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China; University of Chinese Academy of Sciences, Beijing,100049, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China; University of Chinese Academy of Sciences, Beijing,100049, PR China.
authors.forename: Boyan
authors.initials: B
authors.lastname: Liu
authors.affiliation: Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China. Electronic address: bzhao@home.ipe.ac.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D031671:Cistanche / Q000737:chemistry*; D019610:Cytoprotection / Q000187:drug effects*; D000431:Ethanol / Q000633:toxicity; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D013481:Superoxides; D000431:Ethanol
keywords: Antioxidant; Cistanche deserticola; Hepatoprotective activity; Polysaccharides; Preliminary characterizations