title: Methods to Purify and Assay Secretory Pathway Kinases.
abstract: Members of the four-jointed and VLK families of secretory pathway kinases appear to be responsible for the phosphorylation of secreted proteins and proteoglycans. These enzymes have been implicated in many biological processes and mutations in several of these kinases cause human diseases. Here, we describe methods to purify and assay two members of the four-jointed family of secretory kinases: the Fam20C protein kinase and the Fam20B proteoglycan kinase.
authors.affiliation: Department of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX, 75390-9148, USA. vincent.tagliabracci@utsouthwestern.edu.
authors.forename: Vincent S
authors.initials: VS
authors.lastname: Tagliabracci
authors.affiliation: Discovery Bioanalytics, Merck and Co, Rahway, NJ, USA.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China. junyuxiao@pku.edu.cn.|Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China. junyuxiao@pku.edu.cn.
authors.forename: Junyu
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000818:Animals; D047389:Casein Kinase I / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D061987:Sf9 Cells; D018411:Spodoptera
chemical_list: D016326:Extracellular Matrix Proteins; D047389:Casein Kinase I; C524449:FAM20C protein, human
keywords: Amelogenesis imperfecta; Golgi kinase; Hypophosphatemia; Raine syndrome; Secreted kinase