title: Synthesis of adenosine-imprinted microspheres for the recognition of ADP-ribosylated proteins.
abstract: Core-shell structural adenosine-imprinted microspheres were prepared via a two-step procedure. Polystyrene core particles (CP) were firstly prepared via a reversible addition-fragmentation chain transfer (RAFT) polymerization leaving the iniferter on the surface of the cores, then a molecularly imprinted polymer (MIP) shell was synthesized on the surface of the cores by using acrylamide (AAm) as the functional monomer and ethylene glycol dimethacrylate (EGDMA) as the cross-linker. The formation and growth of the MIP layer were seen dependent on the initiator (AIBN), AAm and the polymerization time used within the polymerization. SEM/TEM images showed that the dimensions of the cores and shells were 2μM and 44nm, respectively. The MIP microspheres exhibited a fast rebinding rate within 2h and a maximum adsorption capacity of 177μg per gram for adenosine. The adsorption fitted a Langmuir-Freundlich (LF) isotherm model with a KLFvalue of 41mL/μg and a qmvalue of 177μg/g for the MIP microspheres. The values were larger than those for a non-molecularly imprinted polymer (NIP) particles (5mL/μg and 88μg/g) indicating a better adsorption ability towards adenosine. The MIP microspheres showed a good selectivity for adenosine with a higher adsorption (683nmol/g) for adenosine than that (91nmol/g, 24nmol/g and 54nmol/g) for guanosine, cytidine and uridine respectively. Further experiment proved that the adenosine-imprinted polymer microspheres also had a good selectivity for ADP-ribosylated proteins that the MIP could extract the ADP-ribosylated proteins from the cell extract samples.
authors.affiliation: Department of Food and Pharmaceutical Engineering, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Gong
authors.affiliation: Department of Food and Pharmaceutical Engineering, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Tang
authors.affiliation: Department of Food and Pharmaceutical Engineering, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, China.
authors.forename: Jing Jing
authors.initials: JJ
authors.lastname: Liu
authors.affiliation: Department of Food and Pharmaceutical Engineering, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, China.
authors.forename: Xiang Yu
authors.initials: XY
authors.lastname: You
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Xiao Hong Shan No. 44, Wuhan 430071 China.
authors.forename: Jing
authors.initials: J
authors.lastname: Gu
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Xiao Hong Shan No. 44, Wuhan 430071 China.
authors.forename: Jiao Yu
authors.initials: JY
authors.lastname: Deng
authors.affiliation: Department of Food and Pharmaceutical Engineering, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, China. Electronic address: weihong.xie@mail.hbut.edu.cn.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Xie
mesh_terms: D000241:Adenosine / Q000032:analysis* / Q000302:isolation & purification; D000244:Adenosine Diphosphate / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000737:chemistry; D008863:Microspheres; D054802:Molecular Imprinting / Q000379:methods*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D011108:Polymers; D011137:Polystyrenes; D011506:Proteins; D012710:Serum Albumin, Bovine; D000244:Adenosine Diphosphate; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); D000241:Adenosine
keywords: ADP-ribosylation; Adenosine; Molecularly imprinted polymer; Post-translational modification; Protein recognition