title: TRPA5, an Ankyrin Subfamily Insect TRP Channel, is Expressed in Antennae of Cydia pomonella (Lepidoptera: Tortricidae) in Multiple Splice Variants.
abstract: Transient receptor potential (TRP) channels are an ancient family of cation channels, working as metabotropic triggers, which respond to physical and chemical environmental cues. Perception of chemical signals mediate reproductive behaviors and is therefore an important target for sustainable management tactics against the codling moth Cydia pomonella L. (Lepidoptera: Tortricidae). However, olfactory behavior strongly depends on diel periodicity and correlation of chemical with physical cues, like temperature, and physical cues thus essentially contribute to the generation of behavioral response. From an antennal transcriptome generated by next generation sequencing, we characterized five candidate TRPs in the codling moth. The coding DNA sequence of one of these was extended to full length, and phylogenetic investigation revealed it to be orthologous of the TRPA5 genes, reported in several insect genomes as members of the insect TRPA group with unknown function but closely related to the thermal sensor pyrexia Reverse transcription PCR revealed the existence of five alternate splice forms of CpTRPA5. Identification of a novel TRPA and its splice forms in codling moth antennae open for investigation of their possible sensory roles and implications in behavioral responses related to olfaction.
authors.affiliation: Research and Innovation Centre, Agricultural Entomology - Fondazione Edmund Mach, via E. Mach, 1 38010 San Michele all'Adige, Italy albertomaria.cattaneo@fmach.It.
authors.forename: Alberto Maria
authors.initials: AM
authors.lastname: Cattaneo
authors.affiliation: Department of Zoology, Stockholm University, Svante Arrhenius väg 18 B, Stockholm 106 91, Sweden.
authors.forename: Jonas Martin
authors.initials: JM
authors.lastname: Bengtsson
authors.affiliation: Sorbonne Universités - UPMC, Institute of Ecology & Environmental Sciences of Paris, 4 Place Jussieu 75005 Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Montagné
authors.affiliation: INRA, Institute of Ecology and Environmental Sciences of Paris, Saint Cyr Road, Versailles 78026, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Jacquin-Joly
authors.affiliation: Research and Innovation Centre, Agricultural Entomology - Fondazione Edmund Mach, via E. Mach, 1 38010 San Michele all'Adige, Italy.
authors.forename: Omar
authors.initials: O
authors.lastname: Rota-Stabelli
authors.affiliation: Research and Innovation Centre, Agricultural Entomology - Fondazione Edmund Mach, via E. Mach, 1 38010 San Michele all'Adige, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Salvagnin
authors.affiliation: DeFENS, Department of Food, Nutritional and Environmental Sciences - Università degli Studi di Milano, Via Celoria 2 20133, Milan, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Bassoli
authors.affiliation: Chemical Ecology Unit, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Box 102 SE-23053, Alnarp, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Witzgall
authors.affiliation: Research and Innovation Centre, Agricultural Entomology - Fondazione Edmund Mach, via E. Mach, 1 38010 San Michele all'Adige, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Anfora
mesh_terms: D000818:Animals; D017487:Ankyrins / Q000235:genetics* / Q000378:metabolism; D057506:Arthropod Antennae / Q000378:metabolism; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000378:metabolism; D009036:Moths / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D017487:Ankyrins; D019476:Insect Proteins; D020033:Protein Isoforms
keywords: Cydia pomonella; TRPA subfamily; TRPA5; splice form; transient receptor potential cation channel