title: Xianfanghuomingyin, a Chinese Compound Medicine, Modulates the Proliferation and Differentiation of T Lymphocyte in a Collagen-Induced Arthritis Mouse Model.
abstract: In traditional Chinese medicine (TCM), xianfanghuomingyin (XFHM) is used to treat autoimmune diseases, including rheumatoid arthritis (RA). Here, we studied the mechanisms underlying its treatment effects, especially its anti-inflammatory effects in a collagen-induced arthritis (CIA) mouse model. We found that cartilage destruction and pannus formation were alleviated by treatment with XFHM. The abnormal differentiation of Th1 and Th17 cells was downregulated significantly by XFHM, and Th2 and Treg cells were upregulated. Moreover, the expression levels of specific cytokines and transcription factors related to Th1 cells (interferon γ [IFNγ], T-bet) and Th17 cells (interleukin- [IL-] 17) and the nuclear receptor retinoic acid receptor-related orphan receptor-gamma (RORγ) were downregulated. Serum IL-4 and GATA-3, which contribute to Th2 cells differentiation, increased significantly after XFHM administration. These results indicate that XFHM can restore the balance of T lymphocytes and reestablish the immunological tolerance to inhibit autoinflammatory disorder of RA. Taken together, XFHM can be used as a complementary or alternative traditional medicine to treat RA.
authors.affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Bo
authors.identifier: 0000-0002-8608-3542
authors.initials: B
authors.lastname: Nie
authors.affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wei
authors.affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Rheumatology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Lixia
authors.identifier: 0000-0001-9411-7776
authors.initials: L
authors.lastname: Lou
authors.affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Dong
authors.affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Aiming
authors.identifier: 0000-0002-2746-5532
authors.initials: A
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Dongmei
authors.identifier: 0000-0002-3111-8728
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Lingqun
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Jiuli
authors.identifier: 0000-0002-3634-0416
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Limin
authors.identifier: 0000-0002-9207-1169
authors.initials: L
authors.lastname: Chai