title: An enzymatic approach to configurationally rare trans-androsteronyl-α-glucoside and Its potential anticancer application.
abstract: Enzymatic glycosylation of sterols/steroids with glycosyltransferase HP0421 shows protein plasticity on generation of configurationally rare steryl-α-glucosides. Investigation of trans-androsteronyl-α-glucoside on tamoxifen-treated MCF-7 breast cancer cells shows dose-dependent depression of cell viability and enhanced drug effectiveness, illustrating a new avenue for the production of novel steryl-α-glucosides with useful biological activities.
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan, R.O.C.
authors.forename: Feng-Pai
authors.initials: FP
authors.lastname: Chou
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan, R.O.C.
authors.forename: Chia-Tse
authors.initials: CT
authors.lastname: Tsai
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan, R.O.C.
authors.forename: Ya-Sheng
authors.initials: YS
authors.lastname: Chiou
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan, R.O.C.
authors.forename: Yi-Ju
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan, R.O.C.
authors.forename: Meng-Erh
authors.initials: ME
authors.lastname: Li
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan, R.O.C.
authors.forename: Ting-Wei
authors.initials: TW
authors.lastname: Guo
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan, R.O.C.
authors.forename: Jason WenJay
authors.initials: JW
authors.lastname: Lyu
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan, R.O.C.
authors.forename: Sheng-Hao
authors.initials: SH
authors.lastname: Chou
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan, R.O.C.
authors.forename: Tung-Kung
authors.initials: TK
authors.lastname: Wu
mesh_terms: D000738:Androsterone / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry* / Q000494:pharmacology; D011487:Protein Conformation
chemical_list: D000970:Antineoplastic Agents; D005960:Glucosides; D000738:Androsterone
keywords: 
  Helicobacter pylori
  ; MCF-7 breast cancer cell; glycosyltransferase; steroid α-glucoside; sterol