title: A Rapid TALEN Assembly Protocol.
abstract: Owing to their modular and highly specific DNA recognition mode, transcription activator-like effector nucleases (TALENs) have been rapidly adopted by the scientific community for the purpose of generating site-specific double-strand breaks (DSBs) on a DNA molecule. A pair of TALENs can be used to produce random insertions or deletions of various lengths via nonhomologous end-joining or together with a homologous donor DNA to induce precise sequence alterations by homologous recombination (HR). Here, we describe a method for TALEN assembly (easyT) and a strategy for genome engineering via HR.
authors.affiliation: Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zürich, Mattenstrasse 26, 4058, Basel, Switzerland. arslan.akmammedov@bsse.ethz.ch.
authors.forename: Arslan
authors.initials: A
authors.lastname: Akmammedov
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1 Bunkyo-ku, 113-8654, Tokyo, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Katsuyama
authors.affiliation: Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zürich, Mattenstrasse 26, 4058, Basel, Switzerland.|Faculty of Science, University of Basel, Klingelbergstrasse 50, 4056, Basel, Switzerland.
authors.forename: Renato
authors.initials: R
authors.lastname: Paro
mesh_terms: D053903:DNA Breaks, Double-Stranded; D059766:DNA End-Joining Repair / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D059765:Homologous Recombination / Q000235:genetics*; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D000069896:Transcription Activator-Like Effector Nucleases / Q000235:genetics*
chemical_list: D000069896:Transcription Activator-Like Effector Nucleases
keywords: Genome engineering; Site-specific gene integration; TALEN