title: Chitosan-iron oxide nanocomposite based electrochemical aptasensor for determination of malathion.
abstract: An electrochemical aptasensor based on chitosan-iron oxide nanocomposite (CHIT-IO) film deposited on fluorine tin Oxide (FTO) was developed for the detection of malathion. Iron oxide nanoparticles were prepared by co-precipitation method and characterized by Transmission electron microscopy and UV-Visible spectroscopy. The biotinylated DNA aptamer sequence specific to the malathion was immobilized onto the iron oxide doped-chitosan/FTO electrode by using streptavidin as linking molecule. Various characterization studies like Field Emission-Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR), and Electrochemical studies were performed to attest the successful fabrication of bioelectrodes. Experimental parameters like aptamer concentration, response time, stability of electrode and reusability studies were optimized. Aptamer immobilized chitosan-iron oxide nanocomposite (APT/SA/CHIT-IO/FTO) bioelectrodes exhibited LOD of about 0.001 ng/mL within 15 min and spike-in studies revealed about 80-92% recovery of malathion from the lettuce leaves and soil sample.
authors.affiliation: Department of Biochemistry, Panjab University, Chandigarh 160014, India. Electronic address: nirmalprabhakar@gmail.com.
authors.forename: Nirmal
authors.initials: N
authors.lastname: Prabhakar
authors.affiliation: Department of Biochemistry, Panjab University, Chandigarh 160014, India.
authors.forename: Himkusha
authors.initials: H
authors.lastname: Thakur
authors.affiliation: Department of Biochemistry, Panjab University, Chandigarh 160014, India.
authors.forename: Anu
authors.initials: A
authors.lastname: Bharti
authors.affiliation: Department of Biochemistry, Panjab University, Chandigarh 160014, India.
authors.forename: Navpreet
authors.initials: N
authors.lastname: Kaur
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D005290:Ferric Compounds / Q000737:chemistry*; D005461:Fluorine / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D008294:Malathion / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D053761:Nanocomposites / Q000737:chemistry*; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D005290:Ferric Compounds; D017971:Tin Compounds; C000499:ferric oxide; D005461:Fluorine; D048271:Chitosan; C045358:stannic oxide; D008294:Malathion
keywords: Aptamer; Chitosan-iron oxide nanocomposite; Electrochemical biosensor; Malathion; Organophosphates