title: Proliferation, behavior, and differentiation of osteoblasts on surfaces of different microroughness.
abstract: Titanium surface roughness is recognized as an important parameter influencing osseointegration. However, studies concerning the effect of well-defined surface topographies of titanium surfaces on osteoblasts have been limited in scope. In the present study we have investigated how Ti surfaces of different micrometer-scale roughness influence proliferation, migration, and differentiation of osteoblasts in-vitro.
  
  
  
  Titanium replicas with surface roughnesses (Ra) of approximately 0, 1, 2, and 4μm were produced and MG-63 osteoblasts were cultured on these surfaces for up to 5 days. The effect of surface micrometer-scale roughness on proliferation, migration in time-lapse microscopy experiments, as well as the expression of alkaline phosphatase, osteocalcin, vascular-endothelial growth factor (VEGF), osteoprotegerin (OPG), and receptor activator of nuclear factor kappa-B ligand (RANKL) were investigated.
  
  
  
  Proliferation of MG-63 cells was found to decrease gradually with increasing surface roughness. However, the highest expression of alkaline phosphatase, osteocalcin and VEGF was observed on surfaces with Ra values of approximately 1 and 2μm. Further increase in surface roughness resulted in decreased expression of all investigated parameters. The cell migration speed measured in time-lapse microscopy experiments was significantly lower on surfaces with a Ra value of about 4μm, compared to those with lower roughness. No significant effect of surface roughness on the expression of OPG and RANKL was observed.
  
  
  
  Thus, surfaces with intermediate Ra roughness values of 1-2μm seem to be optimal for osteoblast differentiation. Neither proliferation nor differentiation of osteoblasts appears to be supported by surfaces with higher or lower Ra values.
authors.affiliation: Division of Conservative Dentistry and Periodontology, School of Dentistry, Medical University of Vienna, Vienna, Austria.
authors.forename: Oleh
authors.initials: O
authors.lastname: Andrukhov
authors.affiliation: Department of Materials, Laboratory for Surface Science and Technology, ETH Zurich, Zurich, Switzerland.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Huber
authors.affiliation: Division of Conservative Dentistry and Periodontology, School of Dentistry, Medical University of Vienna, Vienna, Austria; Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shi
authors.affiliation: Institut Straumann AG, Basel, Switzerland.
authors.forename: Simon
authors.initials: S
authors.lastname: Berner
authors.affiliation: Division of Conservative Dentistry and Periodontology, School of Dentistry, Medical University of Vienna, Vienna, Austria.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Rausch-Fan
authors.affiliation: Division of Conservative Dentistry and Periodontology, School of Dentistry, Medical University of Vienna, Vienna, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Moritz
authors.affiliation: Department of Materials, Laboratory for Surface Science and Technology, ETH Zurich, Zurich, Switzerland.
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Spencer
authors.affiliation: Division of Conservative Dentistry and Periodontology, School of Dentistry, Medical University of Vienna, Vienna, Austria. Electronic address: andreas.schedle@meduniwien.ac.at.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schedle
mesh_terms: D000469:Alkaline Phosphatase; D002454:Cell Differentiation; D049109:Cell Proliferation; D003764:Dental Materials; D006801:Humans; D016348:Osseointegration; D010006:Osteoblasts; D015675:Osteocalcin; D010012:Osteogenesis; D013499:Surface Properties; D014025:Titanium
chemical_list: D003764:Dental Materials; D015675:Osteocalcin; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: Micrometer-scale roughness; Osteoblasts; Osteogenesis; Titanium surface