title: RYP1 gene as a target for molecular diagnosis of histoplasmosis.
abstract: This study analyzed the RYP1 gene as a target for the molecular diagnosis of histoplasmosis. This assay detected fungal DNA in 13/13 blood samples from HIV/AIDS-patients with histoplasmosis. Therefore, the detection of RYP1 gene in whole blood sample is a quick and sensitive test to diagnose histoplasmosis.
authors.affiliation: Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: brilhante@ufc.br.
authors.forename: Raimunda Sâmia Nogueira
authors.initials: RS
authors.lastname: Brilhante
authors.affiliation: Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Glaucia Morgana de Melo
authors.initials: GM
authors.lastname: Guedes
authors.affiliation: Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Giovanna Barbosa
authors.initials: GB
authors.lastname: Riello
authors.affiliation: Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Joyce Fonteles
authors.initials: JF
authors.lastname: Ribeiro
authors.affiliation: Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Lucas Pereira
authors.initials: LP
authors.lastname: Alencar
authors.affiliation: Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Silviane Praciano
authors.initials: SP
authors.lastname: Bandeira
authors.affiliation: Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Débora Souza Collares Maia
authors.initials: DS
authors.lastname: Castelo-Branco
authors.affiliation: Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Jonathas Sales
authors.initials: JS
authors.lastname: Oliveira
authors.affiliation: Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Janaína Maria Maia
authors.initials: JM
authors.lastname: Freire
authors.affiliation: São José Hospital, Fortaleza, Ceará, Brazil.
authors.forename: Jacó Ricarte Lima de
authors.initials: JR
authors.lastname: Mesquita
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Zoilo Pires de
authors.initials: ZP
authors.lastname: Camargo
authors.affiliation: Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Rossana de Aguiar
authors.initials: RA
authors.lastname: Cordeiro
authors.affiliation: Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil; College of Veterinary Medicine, Postgraduate Program in Veterinary Sciences, State University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Marcos Fábio Gadelha
authors.initials: MF
authors.lastname: Rocha
authors.affiliation: Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors.forename: José Júlio Costa
authors.initials: JJ
authors.lastname: Sidrim
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D001483:Base Sequence; D001938:Brazil; D060085:Coinfection / Q000175:diagnosis; D004271:DNA, Fungal / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D018390:Gene Targeting / Q000379:methods*; D006658:Histoplasma / Q000235:genetics* / Q000302:isolation & purification; D006660:Histoplasmosis / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004271:DNA, Fungal
keywords: Histoplasmosis; Molecular diagnosis; RYP1