title: miR-155 Inhibits Nucleus Pulposus Cells' Degeneration through Targeting ERK 1/2.
abstract: We first investigated the difference in microRNA expression between normal NP cells and degenerative NP cells using gene chip. We have found that the expression of ERK1/2 was decreased with overexpression of miR-155 in normal nucleus pulposus cell. Expression of ERK1/2 was increased with inhibition of miR-155. Overexpression or inhibition of miR-155 had no effects on the expression level of mRNA ERK1/2 in nucleus pulposus cell, which showed that miR-155 affected the expression of pERK1/2 after transcription of ERK1/2 mRNA indicating that ERK1/2 was a new target protein regulated by miR-155. In the degeneration of intervertebral disc, inhibited miR-155 decreased the expressions of extracellular main matrix collagen II and glycosaminoglycan and increased expression of ERK1/2. Taken together, our data suggested that miR-155 was the identified miRNA which regulated NP cells degenerated through directly targeting ERK1/2.
authors.affiliation: Guangzhou City Red Cross Hospital, The Fourth Affiliated Hospital of the Medical College, Jinan University, Guangzhou 510220, China.
authors.forename: Dongping
authors.identifier: 0000-0002-2677-6267
authors.initials: D
authors.lastname: Ye
authors.affiliation: Guangzhou City Red Cross Hospital, The Fourth Affiliated Hospital of the Medical College, Jinan University, Guangzhou 510220, China.
authors.forename: Libing
authors.initials: L
authors.lastname: Dai
authors.affiliation: Guangzhou City Red Cross Hospital, The Fourth Affiliated Hospital of the Medical College, Jinan University, Guangzhou 510220, China.
authors.forename: Yicun
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Guangzhou City Red Cross Hospital, The Fourth Affiliated Hospital of the Medical College, Jinan University, Guangzhou 510220, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Qin
authors.affiliation: Guangzhou City Red Cross Hospital, The Fourth Affiliated Hospital of the Medical College, Jinan University, Guangzhou 510220, China.
authors.forename: Han
authors.initials: H
authors.lastname: Xie
authors.affiliation: Guangzhou City Red Cross Hospital, The Fourth Affiliated Hospital of the Medical College, Jinan University, Guangzhou 510220, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Guangzhou City Red Cross Hospital, The Fourth Affiliated Hospital of the Medical College, Jinan University, Guangzhou 510220, China.
authors.forename: Weiguo
authors.identifier: 0000-0002-7206-2652
authors.initials: W
authors.lastname: Liang
mesh_terms: D000293:Adolescent; D000368:Aged; D015415:Biomarkers / Q000378:metabolism*; D015153:Blotting, Western; D016022:Case-Control Studies; D002478:Cells, Cultured; D006801:Humans; D055959:Intervertebral Disc Degeneration / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D035683:MicroRNAs / Q000235:genetics*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D000070614:Nucleus Pulposus / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D015415:Biomarkers; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3