title: Effect of dentin on the antimicrobial efficacy of 3% sodium hypochlorite, 2% chlorhexidine, 17% ethylenediaminetetraacetic acid, and 18% etidronic acid on Candida albicans: An in vitro study.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effect of dentin on the antimicrobial efficacy of 3% sodium hypochlorite, 2% chlorhexidine, 17% EDTA and 18% etidronic acid against C. albicans.
  
  
  METHODS
  Dentin powder was prepared from mandibular first premolar using electrical grinder and sterilized. 3% NaOCl, 2%CHX, 17% EDTA and 18% etidronic acid were tested against C. albicans in the presence and absence of dentin, in eppendorf tubes. Group 1 (presence of dentin):- 100ul dentin powder + 100ul C. albicans suspension + 100ul irrigating solution. Group 2 (absence of dentin):- 100ul C. albicans suspension+ 100ul irrigating solution.
  
  
  METHODS
  - 100ul C. albicans suspension.+ 100ul sterile saline Suspension was thoroughly mixed, submitted for serial dilution upto10-5 after 1 min and colony forming units were counted.
  
  
  RESULTS
  In group 2 (without dentin powder), 3% NaOCl and 2% CHX showed the lowest bacterial count compared to group 1 (with dentin powder). Dentin had a significant inhibitory effect on 3% NaOCl (P < 0.001) and 2% CHX (P<0.001). 17% EDTA showed lowest bacterial count in group 1 (with dentin powder) compared to group 2 (without dentin powder). 18% Etidronic acid showed similar bacterial counts in the both the groups. No reduction was observed in the control group.
  
  
  CONCLUSIONS
  NaOCl & EDTA showed measurable antimicrobial effect even in the presence of dentin which can be promising in the reduction of C. albicans in root canal therapy.
authors.affiliation: Department of Conservative Dentistry and Endodontics, The Oxford Dental College, Bengaluru, Karnataka, India.
authors.forename: Rupali
authors.initials: R
authors.lastname: Karale
authors.affiliation: Department of Conservative Dentistry and Endodontics, The Oxford Dental College, Bengaluru, Karnataka, India.
authors.forename: Kamal Maldebhai
authors.initials: KM
authors.lastname: Odedra
authors.affiliation: Department of Conservative Dentistry and Endodontics, The Oxford Dental College, Bengaluru, Karnataka, India.
authors.forename: A
authors.initials: A
authors.lastname: Srirekha
authors.affiliation: Department of Conservative Dentistry and Endodontics, The Oxford Dental College, Bengaluru, Karnataka, India.
authors.forename: C
authors.initials: C
authors.lastname: Champa
authors.affiliation: Department of Conservative Dentistry and Endodontics, The Oxford Dental College, Bengaluru, Karnataka, India.
authors.forename: Ashwija
authors.initials: A
authors.lastname: Shetty
authors.affiliation: Department of Conservative Dentistry and Endodontics, The Oxford Dental College, Bengaluru, Karnataka, India.
authors.forename: S
authors.initials: S
authors.lastname: Pushpalatha
authors.affiliation: Department of Conservative Dentistry and Endodontics, The Oxford Dental College, Bengaluru, Karnataka, India.
authors.forename: Rini
authors.initials: R
authors.lastname: Sharma
keywords: Candida albicans; chlorhexidine; dentin; ethylenediaminetetraacetic acid; etidronic acid; sodium hypochlorite