title: Bioactivity-guided isolation of biphenanthrenes from Liparis nervosa.
abstract: Three new biphenanthrenes, Liparisphenanthrenes A-C (1-3), along with three known ones were obtained from the ethanolic extract of Liparis nervosa (Orchidaceae) by bioactivity-guided fractionation. Their structures were elucidated on the basis of extensive spectroscopic analysis. All the compounds obtained were tested in vitro for cytotoxic activities against stomach (HGC-27) and colon (HT-29) cancer cell lines. 1, 4 and 5 showed potent cytotoxicities to HGC-27 cell line with IC50values of 8.21-9.95μmol/L, and 1 and 5 also exhibited potent cytotoxic activities to HT-29 cell line with IC50values of 8.53-9.27μmol/L.
authors.affiliation: Medical School, Yangzhou University, Yangzhou 225009, PR China; Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou University, Yangzhou 225009, PR China; Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China. Electronic address: enjoyyz@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.affiliation: Medical School, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Qi-Meng
authors.initials: QM
authors.lastname: Yin
authors.affiliation: Medical School, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Xian-Wen
authors.initials: XW
authors.lastname: Zhang
authors.affiliation: Medical School, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Medical School, Yangzhou University, Yangzhou 225009, PR China; Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou University, Yangzhou 225009, PR China; Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Dong
authors.affiliation: Medical School, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.affiliation: Medical School, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D019073:HT29 Cells; D006801:Humans; D015394:Molecular Structure; D029595:Orchidaceae / Q000737:chemistry*; D010616:Phenanthrenes / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010616:Phenanthrenes
keywords: Biphenanthrene; Cytotoxic activity; Liparis nervosa