title: Molecular transport through large-diameter DNA nanopores.
abstract: DNA-based nanopores are synthetic biomolecular membrane pores, whose geometry and chemical functionality can be tuned using the tools of DNA nanotechnology, making them promising molecular devices for applications in single-molecule biosensing and synthetic biology. Here we introduce a large DNA membrane channel with an ≈4 nm diameter pore, which has stable electrical properties and spontaneously inserts into flat lipid bilayer membranes. Membrane incorporation is facilitated by a large number of hydrophobic functionalizations or, alternatively, streptavidin linkages between biotinylated channels and lipids. The channel displays an Ohmic conductance of ≈3 nS, consistent with its size, and allows electrically driven translocation of single-stranded and double-stranded DNA analytes. Using confocal microscopy and a dye influx assay, we demonstrate the spontaneous formation of membrane pores in giant unilamellar vesicles. Pores can be created both in an outside-in and an inside-out configuration.
authors.affiliation: Physik-Department E14, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.|Zentrum für Nanotechnologie und Nanomaterialien/WSI, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.
authors.forename: Swati
authors.initials: S
authors.lastname: Krishnan
authors.affiliation: Physik-Department E14, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.|Zentrum für Nanotechnologie und Nanomaterialien/WSI, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Ziegler
authors.affiliation: Physik-Department E14, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.|Zentrum für Nanotechnologie und Nanomaterialien/WSI, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.
authors.forename: Vera
authors.initials: V
authors.lastname: Arnaut
authors.affiliation: Zentrum für Nanotechnologie und Nanomaterialien/WSI, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.|Physik-Department E69, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Martin
authors.affiliation: Physik-Department E14, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.|Zentrum für Nanotechnologie und Nanomaterialien/WSI, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.
authors.forename: Korbinian
authors.initials: K
authors.lastname: Kapsner
authors.affiliation: Physik-Department E27, Technische Universität München, James-Franck-Straße 1, 85748 Garching, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Henneberg
authors.affiliation: Physik-Department E27, Technische Universität München, James-Franck-Straße 1, 85748 Garching, Germany.
authors.forename: Andreas R
authors.initials: AR
authors.lastname: Bausch
authors.affiliation: Zentrum für Nanotechnologie und Nanomaterialien/WSI, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.|Physik-Department E69, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.
authors.forename: Hendrik
authors.initials: H
authors.lastname: Dietz
authors.affiliation: Physik-Department E14, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.|Zentrum für Nanotechnologie und Nanomaterialien/WSI, Technische Universität München, Am Coulombwall 4a, 85748 Garching, Germany.
authors.forename: Friedrich C
authors.identifier: http://orcid.org/0000-0003-3829-3446
authors.initials: FC
authors.lastname: Simmel