title: Molecular characterization and evolution analysis of five interleukin-17 receptor genes in large yellow croaker Larimichthys crocea.
abstract: Interleukin-17s (IL-17s) play critical roles in inflammatory response and host defense against extracellular pathogens. IL-17s induce the immune response signaling through the specific IL-17 receptors (IL-17Rs) that consist of five members (IL-17RA to E). In the present work, we have identified the five IL-17R orthologs (LycIL-17Rs) from large yellow croaker Larimichthys crocea. The deduced protein of each LycIL-17R exhibits a typical IL-17R domain architecture, including a signal peptide, the extracellular FNIII domain (IL-17RA/RB/RD) or IL-17_R_N domain (IL-17RC/RE), a transmembrane domain, and a SEFIR domain in cytoplasmic region. In particular, the extracellular regions of teleost IL-17RB are much shorter than those in mammals and lack an FNIII domain (FN2). Phylogenetic tree shows that IL-17Rs are classified into two main groups: IL-17RA/RB/RD group and IL-17RC/RE group, which is distinct from previous proposal that grouped IL-17RB into IL-17RC/RE. The surrounding genes of IL-17Rs are conservatively aligned in genomes between teleosts and mammals. The five LycIL-17Rs were constitutively expressed in all tissues examined, but with different expression patterns. Aeromonas hydrophila infection significantly upregulated LycIL-17RA, RC, RD and RE in both mucosal tissue (gills) and systemic immune tissues (head kidney and spleen), while the increase of LycIL-17RB expression could be detected in gills, indicating that LycIL-17Rs may be involved in host defense against bacterial infection. Thus, these results suggest that teleost IL-17Rs may function in mediating immune response as their mammalian orthologs. To our knowledge, this is the first report of molecular characterization of the five IL-17Rs (IL-17RA/RB/RD and IL-17RC/RE) in teleost fish.
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, PR China; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ding
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, PR China.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Ai
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
authors.forename: Yinnan
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
authors.forename: Jingqun
authors.initials: J
authors.lastname: Ao
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, PR China; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, PR China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China. Electronic address: chenxinhua@tio.org.cn.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Chen
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000818:Animals; D019143:Evolution, Molecular; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary; D010473:Perciformes / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D053722:Receptors, Interleukin-17 / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D029941:Fish Proteins; D053722:Receptors, Interleukin-17
keywords: Expression modulation; IL-17 receptor; Large yellow croaker Larimichthys crocea; Molecular evolution