title: Antioxidant and cytotoxic lignans from the roots of Bupleurum chinense.
abstract: In the search for biologically active compounds from the roots of Bupleurum chinense D C., phytochemical investigation of its ethanol extract led to the isolation and identification of a new 8-O-4' neolignan glucoside, saikolignanoside A (1), along with eight known lignans (2-9). Their structures were determined on the basis of IR, UV, HRESIMS, and NMR spectroscopic analyses. The antioxidant and cytotoxic effects of isolated compounds were evaluated in vitro. The isolated compounds (IC50> 200 μM) did not display 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Whereas compounds 1-2, 5, 7, and 9 exhibited potent 2, 2'-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging properties with IC50values ranging from 8.34 to 15.24 μM, while compounds 3-4, 6, 8 showed moderate properties. In addition, all compounds were evaluated for cytotoxicities against A549, HepG2, U251, Bcap-37, and MCF-7 cell lines. Compounds 5 and 9 (IC50< 51.62 μM) possessed stronger cytotoxic activities against all the tested tumor cell lines, compared with the positive control 5-Fluorouracil.
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , China.|b Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Dan-Qi
authors.initials: DQ
authors.lastname: Li
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , China.|b Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , China.|b Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Le
authors.initials: L
authors.lastname: Zhou
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , China.|b Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Li
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , China.|b Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Qing-Bo
authors.initials: QB
authors.lastname: Liu
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , China.|b Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Wu
authors.affiliation: c Department of Pharmacology , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Yang
authors.affiliation: a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , China.|b Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Shao-Jiang
authors.initials: SJ
authors.lastname: Song
authors.affiliation: c Department of Pharmacology , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Chun-Fu
authors.initials: CF
authors.lastname: Wu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000494:pharmacology; D029972:Bupleurum / Q000737:chemistry*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D061986:MCF-7 Cells; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010851:Picrates / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D017705:Lignans; D010851:Picrates; C058836:benzothiazoline; C004931:1,1-diphenyl-2-picrylhydrazyl; D005472:Fluorouracil
keywords: Bupleurum chinense; antioxidant activities; cytotoxicities; lignans; structural elucidation