title: Application of a quantitative1H-NMR (1H-qNMR) method for the determination of geniposidic acid and acteoside in Plantaginis semen.
abstract: A quantitative1H-NMR method (1H-qNMR) was developed to determine the concentration of acteoside and geniposidic acid in Plantaginis semen, which is an important crude drug for diuretic purposes. The purity of geniposidic acid and acteoside was determined by the ratio of the intensity of the H-3 signal at δ 7.51 ppm or the H-7″ signal at δ 7.58 ppm in methanol-d4to that of a hexamethyldisilane (HMD) signal at 0.04 ppm, respectively. The concentration of HMD was corrected with the International System of Units traceability using potassium hydrogen phthalate of certified reference material grade. The geniposidic acid content in two batches of Plantaginis semen as determined by1H-qNMR was found to be 0.84 and 1.00 %, and the acteoside content was determined to be 0.80 and 0.93 %. We demonstrated that this method is useful for the quantitative analysis of geniposidic acid and acteoside in Plantainis semen.
authors.affiliation: Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan.|National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Tanaka
authors.affiliation: Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan.
authors.forename: Risa
authors.initials: R
authors.lastname: Inagaki
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugimoto
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
authors.affiliation: Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan. anagatsu@kinjo-u.ac.jp.
authors.forename: Akito
authors.identifier: http://orcid.org/0000-0003-3863-0991
authors.initials: A
authors.lastname: Nagatsu
mesh_terms: D018744:DNA, Plant / Q000235:genetics*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry*; D057888:Iridoid Glucosides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry*; D010943:Plantago / Q000235:genetics*; D066244:Proton Magnetic Resonance Spectroscopy; D012639:Seeds / Q000235:genetics*; D059808:Polyphenols
chemical_list: D018744:DNA, Plant; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D057888:Iridoid Glucosides; D010636:Phenols; C058966:geniposidic acid; C058956:acteoside; D059808:Polyphenols
keywords: 1H-qNMR; Acteoside; Geniposidic acid; Plantaginis semen; Reagent purity