title: C-phycocyanin alleviates osteoarthritic injury in chondrocytes stimulated with H2O2and compressive stress.
abstract: During the progression of osteoarthritis (OA), dysregulation of extracellular matrix anabolism, abnormal generation of reactive oxygen species (ROS) and inflammatory cytokines have been shown to accelerate the degradation process of cartilage. The potency of c-phycocyanin (C-PC) to protect cellular components against oxidative stress, along with its anti-inflammation and anti-apoptosis effects, are well documented; however, effects of C-PC on OA are still unclear. In this study, we aimed to investigate the effects of C-PC on OA using H2O2or compression-stimulated OA-like porcine chondrocyte models. The results showed that C-PC had the ability to inhibit ROS production, reverse caspase-3 activity, and reduce apoptosis cell population. C-PC also reversed aggrecan and type II collagen gene expressions after stimulation with 1mM H2O2or 60psi of compression. Inhibition of IL-6 and MMP-13 genes was observed in compression-stimulated chondrocytes but not in H2O2-treated cells. In dimethylmethylene blue assay and alcian blue staining, C-PC maintained the sulfated-glycosaminoglycan (sGAG) content after stimulation with compression. We concluded that C-PC can prevent early signs of OA caused by compressive stress and attenuate H2O2-induced oxidative stress. Therefore, we suggest that C-PC can be used as a potential drug candidate for chronic OA treatment.
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, No. 49, Fanglan Rd., Taipei 10672, Taiwan.
authors.forename: In-Chi
authors.initials: IC
authors.lastname: Young
authors.affiliation: Institute of Pharmacology, College of Medicine, National Taiwan University, No. 1, Sec. 1, Ren-Ai Rd., Taipei 10051, Taiwan.
authors.forename: Sung-Ting
authors.initials: ST
authors.lastname: Chuang
authors.affiliation: Institute of Biomedical Engineering and Nanomedicine, National Health Research Institute, No. 35, Keyan Rd., Miaoli 35053, Taiwan.
authors.forename: Chia-Hsien
authors.initials: CH
authors.lastname: Hsu
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, No. 49, Fanglan Rd., Taipei 10672, Taiwan.
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Sun
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, No. 49, Fanglan Rd., Taipei 10672, Taiwan. Electronic address: double@ntu.edu.tw.
authors.forename: Feng-Huei
authors.initials: FH
authors.lastname: Lin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology; D019245:Compressive Strength; D006861:Hydrogen Peroxide / Q000633:toxicity*; D015850:Interleukin-6 / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D010003:Osteoarthritis / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D010798:Phycocyanin / Q000494:pharmacology*; D013314:Stress, Mechanical; D013552:Swine
chemical_list: D015850:Interleukin-6; D010798:Phycocyanin; D006861:Hydrogen Peroxide; D053148:Caspase 3; D053509:Matrix Metalloproteinase 13
keywords: C-phycocyanin; H(2)O(2); Osteoarthritis