title: Morphologic and transcriptomic assessment of bovine embryos exposed to dietary long-chain fatty acids.
abstract: The main objectives of this study were to determine the influence of diets enriched in α-linolenic, linoleic or oleic acid on the development and transcriptomic profile of embryos collected from dairy cattle. Non-lactating Holstein cows received one of the three diets supplemented with 8% rolled oilseeds: flax (FLX, n = 8), sunflower (SUN, n = 7) or canola (CAN, n = 8). After a minimum 35-day diet adaptation, cows were superovulated, artificially inseminated and ova/embryos recovered non-surgically after 7.5 days. Cows fed FLX had less degenerated embryos and more viable embryos than those fed CAN or SUN. In total, 175 genes were differentially expressed in blastocysts from cows fed FLX than in cows fed CAN or SUN. These differentially expressed genes were mainly involved in cellular growth and proliferation, cellular development, and cell survival and viability. In conclusion, dietary n-3 polyunsaturated fatty acids reduced early embryonic degeneration possibly through improving embryonic cell survival and viability.
authors.affiliation: Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.|Departments of Obstetrics and Gynecology & Cellular and Molecular MedicineInterdisciplinary School of Health Sciences, University of Ottawa, and Chronic Disease Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada.
authors.forename: Reza
authors.initials: R
authors.lastname: Salehi
authors.affiliation: Livestock Research BranchAlberta Agriculture and Forestry, Edmonton, Alberta, Canada.
authors.forename: Marcos G
authors.initials: MG
authors.lastname: Colazo
authors.affiliation: Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Stephen
authors.initials: S
authors.lastname: Tsoi
authors.affiliation: Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Amir
authors.initials: A
authors.lastname: Behrouzi
authors.affiliation: Departments of Obstetrics and Gynecology & Cellular and Molecular MedicineInterdisciplinary School of Health Sciences, University of Ottawa, and Chronic Disease Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada.|Macau Institute for Applied Research in Medicine and HealthState Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China.
authors.forename: Benjamin K
authors.initials: BK
authors.lastname: Tsang
authors.affiliation: Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Michael K
authors.initials: MK
authors.lastname: Dyck
authors.affiliation: Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Masahito
authors.initials: M
authors.lastname: Oba
authors.affiliation: Department of AgriculturalFood and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada divakar.ambrose@gov.ab.ca.|Livestock Research BranchAlberta Agriculture and Forestry, Edmonton, Alberta, Canada.
authors.forename: Divakar J
authors.initials: DJ
authors.lastname: Ambrose
mesh_terms: D000818:Animals; D002417:Cattle; D019587:Dietary Supplements; D004622:Embryo, Mammalian / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*
chemical_list: D005227:Fatty Acids