title: Multiplatform Biomarker Discovery for Bladder Cancer Recurrence Diagnosis.
abstract: Purpose.Nonmuscle invasive bladder cancer (BCa) has a high recurrence rate requiring lifelong surveillance. Urinary biomarkers are promising as simple alternatives to cystoscopy for the diagnosis of recurrent bladder cancer. However, no single marker can achieve the required accuracy. The purpose of this study was to select a multiparameter panel, comprising urinary biomarkers and clinical parameters, for BCa recurrence diagnosis.Experimental Design.Candidate biomarkers were measured in urine samples of BCa patients with recurrence and BCa patients without recurrence. A multiplatform strategy was used for marker quantification comprising a multiplexed microarray and an automated platform for ELISA analysis. A multivariate statistical analysis combined the results from both platforms with the collected clinical data.Results.The best performing combination of biomarkers and clinical parameters achieved an AUC value of 0.91, showing better performance than individual parameters. This panel comprises six biomarkers (cadherin-1, IL-8, ErbB2, IL-6, EN2, and VEGF-A) and three clinical parameters (number of past recurrences, number of BCG therapies, and stage at time of diagnosis).Conclusions.The multiparameter panel could be a useful noninvasive tool for BCa surveillance and potentially impact the clinical management of this disease. Validation of results in an independent cohort is warranted.
authors.affiliation: AXO Science SAS, 34 rue du Mail, 69004 Lyon, France.
authors.forename: Marine
authors.initials: M
authors.lastname: De Paoli
authors.affiliation: AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430 Tulln, Austria.
authors.forename: Selma
authors.initials: S
authors.lastname: Gogalic
authors.affiliation: AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430 Tulln, Austria.
authors.forename: Ursula
authors.initials: U
authors.lastname: Sauer
authors.affiliation: AIT Austrian Institute of Technology GmbH, Konrad-Lorenz-Straße 24, 3430 Tulln, Austria.
authors.forename: Claudia
authors.initials: C
authors.lastname: Preininger
authors.affiliation: Department of Oncology, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Surrey GU2 7WG, UK.
authors.forename: Hardev
authors.initials: H
authors.lastname: Pandha
authors.affiliation: Department of Oncology, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Surrey GU2 7WG, UK.
authors.forename: Guy
authors.initials: G
authors.lastname: Simpson
authors.affiliation: Department of Oncology, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Surrey GU2 7WG, UK.
authors.forename: Andras
authors.initials: A
authors.lastname: Horvath
authors.affiliation: Université de Lyon, Université Lyon 1, CNRS, INSA, CPE-Lyon, ICBMS, UMR 5246, No. 43, boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
authors.forename: Christophe
authors.identifier: 0000-0003-3019-0696
authors.initials: C
authors.lastname: Marquette