title: Black Hoof Medicinal Mushroom Phellinus linteus (Agaricomycetes) Extracts Protect Against Radiation-Induced Hematopoietic Abnormality in Mice.
abstract: We investigated the effects of Phellinus linteus extracts (PLEs) against radiation damage in mice. First, BALB/c mice were irradiated once with γ-rays at 4, 5, 6, or 8 Gy and allowed to recover for 20 days. Results reveal that 8-Gy radiation caused death in 100% of mice on day 13, and 6-Gy radiation caused death in 86.7% of mice (13/15) at the end of the experiment, whereas 4- and 5-Gy radiation did not result in any death. We then used 5-Gy γ-ray radiation to examine the protective effects of PLEs. Mice were orally administered a PLE (500, 1000, and 1500 mg/kg) daily for 2 weeks before radiation and for 6 weeks after radiation. γ-Ray radiation significantly decreased body weight starting from week 2 after radiation. Supplementation with a median and high dose of PLE significantly restored body weights starting at weeks 5 and 3, respectively. The radiation-protective agent WR2721 (200 mg/kg intraperitoneally) restored body weights starting at week 4. White blood cells, platelets, red blood cells, and hemoglobin were significantly decreased by radiation, and PLEs (primarily at high doses) and WR2721 significantly prevented hematologic abnormality. These results suggest that PLE has potential as a radioprotective agent.
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, R.O.C.; Department of Nutrition, Nantou Hospital, Ministry of Health and Welfare, Nantou City, Taiwan, R.O.C.
authors.forename: Shu-Ming
authors.initials: SM
authors.lastname: Huang
authors.affiliation: Department of Anesthesiology, Chi Mei Medical Center, Tainan, Taiwan, R.O.C.
authors.forename: Jen-Yin
authors.initials: JY
authors.lastname: Chen
authors.affiliation: Biotechnology Center, Grape King Inc., Taoyuan, Taiwan, R.O.C.
authors.forename: Chin-Chu
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Oncology, Jan-Ai Hospital-DaLi, Taichung, Taiwan, R.O.C.
authors.forename: Chih-Chung
authors.initials: CC
authors.lastname: Su
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, R.O.C.; Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, R.O.C.
authors.forename: Miao-Lin
authors.initials: ML
authors.lastname: Hu
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D005720:Gamma Rays / Q000009:adverse effects*; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology; D015431:Weight Loss; D014916:Whole-Body Irradiation
chemical_list: D011837:Radiation-Protective Agents