title: MicroRNA-330-5p negatively regulates ITGA5 expression in human colorectal cancer.
abstract: Colorectal cancer (CRC), one of the most prevalent malignant cancers, has high rates pf incidence and is the fourth leading cause of cancer-related deaths for both men and women worldwide. MicroRNAs (miRNAs) play critical roles in the development of various types of cancers. miRNA‑330-5p has been implicated in the progression of prostate, neuronal and pancreatic cancers by regulating proliferation, migration, invasion and epithelial-mesenchymal transition of cells. The purpose of the present study was to investigate the expression of miR-330-5p in CRC and identify its target gene(s) that may act in CRC tumorigenesis. We found that miR-330-5p expression was significantly lower in CRC tissues than that in adjacent non-tumorous tissues. Furthermore, we identified integrin α5 (ITGA5) as a new target of miR-330-5p and found that it inhibits ITGA5 expression by directly binding to the 3' untranslated region of ITGA5 mRNA. These results suggest that downregulation of miR-330-5p expression may affect CRC development via modulation of ITGA5 expression.
authors.affiliation: Department of Medical Life Sciences, The Catholic University of Korea, Seoul, Seochogu 137-701, Republic of Korea.
authors.forename: Hye-In
authors.initials: HI
authors.lastname: Yoo
authors.affiliation: Department of Medical Life Sciences, The Catholic University of Korea, Seoul, Seochogu 137-701, Republic of Korea.
authors.forename: Bong-Kyu
authors.initials: BK
authors.lastname: Kim
authors.affiliation: Department of Medical Life Sciences, The Catholic University of Korea, Seoul, Seochogu 137-701, Republic of Korea.
authors.forename: Sungjoo Kim
authors.initials: SK
authors.lastname: Yoon
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000235:genetics; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D039503:Integrin alpha6 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D020413:3' Untranslated Regions; C588002:ITGA6 protein, human; D039503:Integrin alpha6; C570805:MIRN330 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger