title: Structural Elucidation of a Novel Polysaccharide from Pseudostellaria heterophylla and Stimulating Glucose Uptake in Cells and Distributing in Rats by Oral.
abstract: The semi-refined polysaccharide of Pseudostellaria heterophylla is a complex polysaccharide that exhibits significantly hypoglycemic activities. A novel homogeneous polysaccharide, named as H-1-2, was isolated from the semi-refined polysaccharide. The mean molecular weight of H-1-2 was 1.4 × 10⁴ Da and it was only composed of d-glucose monosaccharide. Structure elucidation indicated that H-1-2 contains pyranride, and has the characteristics of the α-iso-head configuration, a non-reducing end (T-), 4-, 1,6-, and 1,4,6-connection, in all four ways to connect glucose. H-1-2 was a type of glucan, where chemical combination exists in the main chain between 1→4 linked glucose, and contains a small amount of 1,6-linked glucose, which was in the branched chain. In vitro HepG2, 3T3-L1, and L6 cells were used to assess cellular glucose consumption and cellular glucose uptake by glucose oxidase, and the transport of 2-NBDG fluorescence probe results showed that H-1-2 could clearly increase glucose uptake and utilization in muscle and adipose cells, which is beneficial to screen for in the discovery of anti-diabetes lead compounds. H-1-2 was labeled with radioisotopes ((99m)Tc-pertechnetate). (99m)Tc-labeled-H-1-2 was performed by SPECT/CT analysis images after oral administration in rats. At 4 h post ingestion, about 50% of the radioactivity was observed in the intestine. No significant radioactivity was found in the heart, liver, and kidney, conjecturing that absorption of (99m)Tc-labeled H-1-2 might, via intestinal mucosa, be absorbed into systemic circulation. This problem, as to whether the polysaccharide is absorbed orally, will need further examination.
authors.affiliation: The Institute of Drug Research, Fujian Academy of Traditional Chinese Medicine, Fuzhou 350003, China. hzf_ketizu@163.com.|The College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. hzf_ketizu@163.com.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Chen
authors.affiliation: The College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. pws@fjtcm.edu.cn.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Pang
authors.affiliation: The College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. 13860662716@163.com.
authors.forename: Wentao
authors.initials: W
authors.lastname: Shi
authors.affiliation: The College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. 13905915817@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: The Institute of Drug Research, Fujian Academy of Traditional Chinese Medicine, Fuzhou 350003, China. cld_ketizu@163.com.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Kan
authors.affiliation: The Institute of Drug Research, Fujian Academy of Traditional Chinese Medicine, Fuzhou 350003, China. hzd_fjtcmyjy@163.com.
authors.forename: Zhaodong
authors.initials: Z
authors.lastname: He
authors.affiliation: The Institute of Drug Research, Fujian Academy of Traditional Chinese Medicine, Fuzhou 350003, China. huj@fjtcm.edu.cn.|The College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. huj@fjtcm.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Hu
mesh_terms: D041721:3T3-L1 Cells; D000284:Administration, Oral; D000818:Animals; D029748:Caryophyllaceae / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism*; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats
chemical_list: D007004:Hypoglycemic Agents; D011134:Polysaccharides; D005947:Glucose
keywords: Pseudostellaria heterophylla; a novel polysaccharide; distribution taken orally; glucose uptake; structure