title: Porcine milk-derived exosomes promote proliferation of intestinal epithelial cells.
abstract: Milk-derived exosomes were identified as a novel mechanism of mother-to-child transmission of regulatory molecules, but their functions in intestinal tissues of neonates are not well-studied. Here, we characterized potential roles of porcine milk-derived exosomes in the intestinal tract. In vitro, treatment with milk-derived exosomes (27 ± 3 ng and 55 ± 5 ng total RNA) significantly promoted IPEC-J2 cell proliferation by MTT, CCK8, EdU fluorescence and EdU flow cytometry assays. The qRT-PCR and Western blot analyses indicated milk-derived exosomes (0.27 ± 0.03 μg total RNA) significantly promoted expression of CDX2, IGF-1R and PCNA, and inhibited p53 gene expression involved in intestinal proliferation. Additionally, six detected miRNAs were significantly increased in IPEC-J2 cell, while FAS and SERPINE were significantly down-regulated relative to that in control. In vivo, treated groups (0.125 μg and 0.25 μg total RNA) significantly raised mice' villus height, crypt depth and ratio of villus length to crypt depth of intestinal tissues, significantly increased CDX2, PCNA and IGF-1R' expression and significantly inhibited p53' expression. Our study demonstrated that milk-derived exosomes can facilitate intestinal cell proliferation and intestinal tract development, thus giving a new insight for milk nutrition and newborn development and health.
authors.affiliation: National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.affiliation: National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Mei-Ying
authors.initials: MY
authors.lastname: Xie
authors.affiliation: National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Jia-Jie
authors.initials: JJ
authors.lastname: Sun
authors.affiliation: National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Rui-Song
authors.initials: RS
authors.lastname: Ye
authors.affiliation: National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Cheng
authors.affiliation: National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Rui-Ping
authors.initials: RP
authors.lastname: Sun
authors.affiliation: National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Wei
authors.affiliation: National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: De-Lin
authors.initials: DL
authors.lastname: Lin
authors.affiliation: National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Qing-Yan
authors.initials: QY
authors.lastname: Jiang
authors.affiliation: National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Qian-Yun
authors.initials: QY
authors.lastname: Xi
authors.affiliation: National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D055354:Exosomes / Q000737:chemistry*; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D008892:Milk / Q000737:chemistry*; D013552:Swine