title: Human γδ T cells augment antigen presentation inListeria Monocytogenesinfection.
abstract: Circulating γδ T cells in healthy individuals rapidly respond to bacterial and viral pathogens. Many studies have demonstrated that γδ T cells are activated and expanded byListeria monocytogenes(L.monocytogenes), a foodborne bacterial pathogen with high fatality rates. However, the roles of γδ T cells duringL.monocytogenesinfection are not clear. In the present study, we characterized the morphological characteristics of phagocytosis in γδ T cells afterL.monocytogenesinfection using transmission electron microscopy. Results show activation markers including HLA-DR and lymph node-homing receptor CCR7 on γδ T cells were upregulated after stimulation viaL.monocytogenes. Significant proliferation and differentiation of primary αβ T cells was also observed after co-culture of peripheral blood mononuclear cells with γδ T cells anteriorly stimulated byL.monocytogenes.L.monocytogenesinfection decreased the percentage of γδ T cells in mouse IELs and increased MHC-II expression on the surface of γδ T cellsin vivo. Our findings shed light on antigen presentation of γδ T cells duringL.monocytogenesinfection.
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College.|State Key Laboratory of Medical Molecular Biology, Beijing, China.
authors.forename: Yuli
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College.|State Key Laboratory of Medical Molecular Biology, Beijing, China.|Child Development and Disease Research Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences.
authors.forename: Huaishan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College.|State Key Laboratory of Medical Molecular Biology, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College.|State Key Laboratory of Medical Molecular Biology, Beijing, China.|Child Development and Disease Research Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College.
authors.forename: Lianxian
authors.initials: L
authors.lastname: Cui
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College.|State Key Laboratory of Medical Molecular Biology, Beijing, China.|Child Development and Disease Research Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College.|State Key Laboratory of Medical Molecular Biology, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
keywords: Listeria monocytogenes; antigen presenting cell; γδ T cells