title: Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells.
abstract: Exposure to coal and coal ashes can cause harmful effects in in vitro and in vivo systems, mainly by the induction of oxidative damage. The aim of this work was to assess cytotoxic and genotoxic effects using the V79 cell line treated with coal and coal fly ash particles derived from a coal power plant located in Santa Catarina, Brazil. Two coal samples (COAL11 and COAL16) and two coal fly ash samples (CFA11 and CFA16) were included in this study. COAL16 was co-firing with a mixture of fuel oil and diesel oil. The comet assay data showed that exposure of V79 cells to coal and coal fly ash particles induced primary DNA lesions. Application of lesion-specific endonucleases (FPG and ENDO III) demonstrated increased DNA effects indicating the presence of high amounts of oxidative DNA lesions. The cytokinesis-block micronucleus cytome assay analysis showed that exposure of V79 cells to high concentrations of coal and coal fly ash particles induced cytotoxic effects (apoptosis and necrosis) and chromosomal instability (nucleoplasmic bridges, nuclear buds, and micronucleus (MN) formation). These results may be associated with compounds contained in the surface of the particles as hazardous elements, ultrafine/nanoparticles, and polycyclic aromatic hydrocarbons (PAHs) which were detected in the samples. Graphical abstract ᅟ.
authors.affiliation: Departamento de Biofísica, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. grethelleon@gmail.com.|Unidad de Investigación, Desarrollo e Innovación en Genética y Biología Molecular, Universidad Simón Bolívar, Barranquilla, Colombia. grethelleon@gmail.com.
authors.forename: Grethel
authors.initials: G
authors.lastname: León-Mejía
authors.affiliation: Research group in Environmental Management and Sustainability, Faculty of Environmental Sciences, Universidad de la Costa, Barranquilla, Colombia.|Universidade do Sul de Santa Catarina, Pró-Reitoria de Ensino, de Pesquisa e de Extensão, UNISUL -Universidade do Sul de Santa Catarina Pedra Branca, Palhoça, SC, 88137900, Brazil.
authors.forename: Luis F O
authors.initials: LF
authors.lastname: Silva
authors.affiliation: Universidade do Sul de Santa Catarina, Pró-Reitoria de Ensino, de Pesquisa e de Extensão, UNISUL -Universidade do Sul de Santa Catarina Pedra Branca, Palhoça, SC, 88137900, Brazil.
authors.forename: Matheus S
authors.initials: MS
authors.lastname: Civeira
authors.affiliation: Universidade do Sul de Santa Catarina, Pró-Reitoria de Ensino, de Pesquisa e de Extensão, UNISUL -Universidade do Sul de Santa Catarina Pedra Branca, Palhoça, SC, 88137900, Brazil.
authors.forename: Marcos L S
authors.initials: ML
authors.lastname: Oliveira
authors.affiliation: InnVitro Pesquisa e Desenvolvimento, Porto Alegre, RS, Brazil.
authors.forename: Miriana
authors.initials: M
authors.lastname: Machado
authors.affiliation: InnVitro Pesquisa e Desenvolvimento, Porto Alegre, RS, Brazil.
authors.forename: Izabel Vianna
authors.initials: IV
authors.lastname: Villela
authors.affiliation: Novartis Pharma AG, Basel, Switzerland.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hartmann
authors.affiliation: Laboratório de Genética Toxicológica, Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil.
authors.forename: Suziane
authors.initials: S
authors.lastname: Premoli
authors.affiliation: Laboratório de Genética Toxicológica, Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil.
authors.forename: Dione Silva
authors.initials: DS
authors.lastname: Corrêa
authors.affiliation: Laboratório de Genética Toxicológica, Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Da Silva
authors.affiliation: Departamento de Biofísica, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. pegas@cbiot.ufrgs.br.
authors.forename: João Antônio Pêgas
authors.initials: JA
authors.lastname: Henriques
mesh_terms: D001938:Brazil; D002460:Cell Line; D003031:Coal / Q000032:analysis / Q000633:toxicity; D060729:Coal Ash / Q000032:analysis / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D006801:Humans; D053758:Nanoparticles / Q000032:analysis / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity; D011210:Power Plants
chemical_list: D003031:Coal; D060729:Coal Ash; D011084:Polycyclic Aromatic Hydrocarbons
keywords: Coal; Coal fly ashes; Comet assay; DNA oxidative damage; Micronucleus assay