title: Immune responses of a meningococcal A + W outer membrane vesicle (OMV) vaccine with and without aluminium hydroxide adjuvant in two different mouse strains.
abstract: Meningococci (Neisseria meningiditis) of serogroups A and W have caused large epidemics of meningitis in sub-Saharan Africa for decades, and affordable and multivalent vaccines, effective in all age groups, are needed. A bivalent serogroup A and W (A + W) meningococcal vaccine candidate consisting of deoxycholate-extracted outer membrane vesicles (OMV) from representative African disease isolates was previously found to be highly immunogenic in outbred mice when formulated with the adjuvant aluminium hydroxide (AH). OMV has been shown to have inherent adjuvant properties. In order to study the importance of AH and genetical differences between mice strains on immune responses, we compared the immunogenicity of the A + W OMV vaccine when formulated with or without AH in inbred C57BL/6J and BALB/cJ mice (Th1 and Th2 dominant strains, respectively). The immunogenicity of the vaccine was found to be comparable in the two mice strains despite their different immune profiles. Adsorption to AH increased anti-OMV IgG levels and serum bactericidal activity (SBA). The immune responses were increased by each dose for the adsorbed vaccine, but the third dose did not significantly improve the immunogenicity further. Thus, a vaccine formulation with the A and W OMV will likely benefit from including AH as adjuvant.
authors.affiliation: Norwegian Institute of Public Health (NIPH), Domain for Infection Control and Environmental Health, Oslo, Norway. gro.tunheim@fhi.no.
authors.forename: Gro
authors.initials: G
authors.lastname: Tunheim
authors.affiliation: Norwegian Institute of Public Health (NIPH), Domain for Infection Control and Environmental Health, Oslo, Norway.|Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.
authors.forename: Marianne
authors.initials: M
authors.lastname: Arnemo
authors.affiliation: Norwegian Institute of Public Health (NIPH), Domain for Infection Control and Environmental Health, Oslo, Norway.
authors.forename: Lisbeth M
authors.initials: LM
authors.lastname: Naess
authors.affiliation: Norwegian Institute of Public Health (NIPH), Domain for Infection Control and Environmental Health, Oslo, Norway.
authors.forename: Gunnstein
authors.initials: G
authors.lastname: Norheim
authors.affiliation: Finlay Institute, Havana, Cuba.
authors.forename: Luis
authors.initials: L
authors.lastname: Garcia
authors.affiliation: Finlay Institute, Havana, Cuba.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cardoso
authors.affiliation: Finlay Institute, Havana, Cuba.
authors.forename: Aleida
authors.initials: A
authors.lastname: Mandiarote
authors.affiliation: Finlay Institute, Havana, Cuba.
authors.forename: Domingo
authors.initials: D
authors.lastname: Gonzalez
authors.affiliation: Norwegian Institute of Public Health (NIPH), Domain for Infection Control and Environmental Health, Oslo, Norway.
authors.forename: Kalpana
authors.initials: K
authors.lastname: Sinnadurai
authors.affiliation: Norwegian Institute of Public Health (NIPH), Domain for Infection Control and Environmental Health, Oslo, Norway.
authors.forename: Åse-Karine
authors.initials: ÅK
authors.lastname: Fjeldheim
authors.affiliation: Norwegian Institute of Public Health (NIPH), Domain for Infection Control and Environmental Health, Oslo, Norway.
authors.forename: Karin
authors.initials: K
authors.lastname: Bolstad
authors.affiliation: Norwegian Institute of Public Health (NIPH), Domain for Infection Control and Environmental Health, Oslo, Norway.
authors.forename: Einar
authors.initials: E
authors.lastname: Rosenqvist
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D017954:Africa South of the Sahara; D000536:Aluminum Hydroxide / Q000008:administration & dosage*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001770:Blood Bactericidal Activity; D055252:Cell-Derived Microparticles / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D022401:Meningococcal Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D038381:Neisseria meningitidis, Serogroup A / Q000276:immunology*; D038582:Neisseria meningitidis, Serogroup W-135 / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D022401:Meningococcal Vaccines; D000536:Aluminum Hydroxide
keywords: BALB/c; C57BL/6; Meningococcal vaccine; aluminium hydroxide; outer membrane vesicles