title: In Vitro and In Vivo Anti-Helicobacter Activities of Eryngium foetidum (Apiaceae), Bidens pilosa (Asteraceae), and Galinsoga ciliata (Asteraceae) against Helicobacter pylori.
abstract: This study was performed to evaluate the antimicrobial activities of extracts of Bidens pilosa, Galinsoga ciliata, and Eryngium foetidum against 6 clinical strains of Helicobacter pylori in vitro and in vivo. Broth microdilution method was used in vitro. In vivo, Swiss mice were inoculated with H. pylori and divided into 5 groups; the control group received the vehicle and the four others received 125, 250, and 500 mg/kg of methanol extract of Eryngium foetidum and ciprofloxacin (500 mg/kg) for 7 days, respectively. Helicobacter pylori colonization and number of colonies in gastric biopsies culture were assessed on days 1 and 7 after treatment. The lowest MIC value (64 μg/mL) and the best spectrum of bactericidal effect (MBC/MIC = 1) were obtained with the methanol extract of Eryngium foetidum. The number of H. pylori infected animals was 17% (plant-extract) and 0% (ciprofloxacin) compared to 100% for the infected untreated group. Plant-extract (381.9 ± 239.5 CFU) and ciprofloxacin (248 ± 153.2 CFU) significantly reduced bacterial load in gastric mucosa compared to untreated, inoculated mice (14350 ± 690 CFU). Conclusion. The present data provided evidence that methanol extract of Eryngium foetidum could be a rich source of metabolites with antimicrobial activity to fight Helicobacter pylori infections.
authors.affiliation: Microbiology and Pharmacology Laboratory, Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
authors.forename: Laure Brigitte
authors.identifier: 0000-0002-6447-1413
authors.initials: LB
authors.lastname: Kouitcheu Mabeku
authors.affiliation: Microbiology and Pharmacology Laboratory, Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Eyoum Bille
authors.affiliation: Gastroenterology Department, Laquintinie Hospital of Douala, P.O. Box 4035, Douala, Cameroon.
authors.forename: Eveline
authors.initials: E
authors.lastname: Nguepi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D019659:Asteraceae / Q000737:chemistry*; D031176:Bidens / Q000737:chemistry*; D001706:Biopsy; D002939:Ciprofloxacin / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D031049:Eryngium / Q000737:chemistry*; D005753:Gastric Mucosa / Q000378:metabolism; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori / Q000187:drug effects*; D051379:Mice; D008826:Microbial Sensitivity Tests; D064209:Phytochemicals / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D013234:Stem Cells
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D064209:Phytochemicals; D010936:Plant Extracts; D002939:Ciprofloxacin