title: Identification of novel antigens contributing to autoimmunity in cardiovascular diseases.
abstract: In myocarditis and dilated cardiomyopathy (DCM) patients the immune system may play an important role in disease progression. In this study, we aimed to identify new antigens as a target for autoimmune response that might play a crucial role in these diseases. Therefore, a peptide-array was used to investigate antibody binding profiles in patients with autoimmune myocarditis or DCM compared to healthy controls and thus to identify disease relevant antigens. To analyze the pathogenicity of the identified antigens, an experimental autoimmune myocarditis (EAM) model was used. Hereby, 3 peptide sequences, derived from myosin-binding-protein-C (MYBPC) fast-type, RNA-binding-protein 20 (RBM20), and dystrophin, showed pathogenic effects on the myocardium of mice. In summary, 3 potentially cardiopathogenic peptides (MYBPC fast-type, RBM20, dystrophin) were identified. Thus, this study could serve as a basis for future investigations aimed at determining further antigens leading to pathogenic effects on the myocardium of DCM as well as myocarditis patients.
authors.affiliation: Department of Cardiology, University of Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Müller
authors.affiliation: Department of Cardiology, University of Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Mariella
authors.initials: M
authors.lastname: Bockstahler
authors.affiliation: Department of Cardiology, University of Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Georgi
authors.initials: G
authors.lastname: Hristov
authors.affiliation: Department of Clinical Statistics, Biomathematics, Information Processing, University of Heidelberg/Mannheim, 68167 Mannheim, Germany.
authors.forename: Christel
authors.initials: C
authors.lastname: Weiß
authors.affiliation: Department of Cardiology, University of Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fischer
authors.affiliation: Department of Cardiac Surgery, University of Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Sevil
authors.initials: S
authors.lastname: Korkmaz-Icöz
authors.affiliation: Department of Cardiology, University of Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Giannitsis
authors.affiliation: Department of Cardiology and Pneumology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany; DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, University of Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Poller
authors.affiliation: Department of Cardiology and Pneumology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.
authors.forename: Heinz-Peter
authors.initials: HP
authors.lastname: Schultheiss
authors.affiliation: Department of Cardiology, University of Heidelberg, 69120 Heidelberg, Germany; DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, University of Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Hugo A
authors.initials: HA
authors.lastname: Katus
authors.affiliation: Department of Cardiology, University of Heidelberg, 69120 Heidelberg, Germany; DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, University of Heidelberg, 69120 Heidelberg, Germany. Electronic address: ziya.kaya@med.uni-heidelberg.de.
authors.forename: Ziya
authors.initials: Z
authors.lastname: Kaya
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000276:immunology* / Q000473:pathology; D015551:Autoimmunity; D002311:Cardiomyopathy, Dilated / Q000276:immunology* / Q000473:pathology; D016207:Cytokines / Q000235:genetics; D005260:Female; D006801:Humans; D051379:Mice; D009205:Myocarditis / Q000276:immunology* / Q000473:pathology; D009206:Myocardium / Q000276:immunology / Q000473:pathology; D010455:Peptides / Q000276:immunology; D012333:RNA, Messenger
chemical_list: D001324:Autoantigens; D016207:Cytokines; D010455:Peptides; D012333:RNA, Messenger
keywords: Autoantigen; Autoimmunity; Dilated cardiomyopathy; Experimental autoimmune myocarditis