title: Detection of Soluble ED-A(+) Fibronectin and Evaluation as Novel Serum Biomarker for Cardiac Tissue Remodeling.
abstract: Background and Aims. Fibronectin containing the extra domain A (ED-A(+) Fn) was proven to serve as a valuable biomarker for cardiac remodeling. The study was aimed at establishing an ELISA to determine ED-A(+) Fn in serum of heart failure patients. Methods. ED-A(+) Fn was quantified in serum samples from 114 heart failure patients due to ischemic (ICM, n = 44) and dilated (DCM, n = 39) cardiomyopathy as well as hypertensive heart disease (HHD, n = 31) compared to healthy controls (n = 12). Results. In comparison to healthy volunteers, heart failure patients showed significantly increased levels of ED-A(+) Fn (p < 0.001). In particular in ICM patients there were significant associations between ED-A(+) Fn serum levels and clinical parameters, for example, increased levels with rising NYHA class (p = 0.013), a negative correlation with left ventricular ejection fraction (p = 0.026, r: -0.353), a positive correlation with left atrial diameter (p = 0.008, r: 0.431), and a strong positive correlation with systolic pulmonary artery pressure (p = 0.002, r: 0.485). In multivariate analysis, ED-A(+) Fn was identified as an independent predictor of an ischemic heart failure etiology. Conclusions. The current study could clearly show that ED-A(+) Fn is a promising biomarker in cardiovascular diseases, especially in heart failure patients due to an ICM. We presented a valid ELISA method, which could be applied for further studies investigating the value of ED-A(+) Fn.
authors.affiliation: Jena University Hospital, Department of Internal Medicine I, 07747 Jena, Germany; Swiss Federal Institute of Technology, Department of Chemistry and Applied Biosciences, 8093 Zurich, Switzerland.
authors.forename: Barbara
authors.identifier: 0000-0002-7341-6117
authors.initials: B
authors.lastname: Ziffels
authors.affiliation: Jena University Hospital, Department of Internal Medicine I, 07747 Jena, Germany.
authors.forename: Johanna
authors.initials: J
authors.lastname: Ospel
authors.affiliation: Jena University Hospital, Department of Internal Medicine I, 07747 Jena, Germany.
authors.forename: Katja
authors.identifier: 0000-0001-7859-5444
authors.initials: K
authors.lastname: Grün
authors.affiliation: Swiss Federal Institute of Technology, Department of Chemistry and Applied Biosciences, 8093 Zurich, Switzerland.
authors.forename: Dario
authors.initials: D
authors.lastname: Neri
authors.affiliation: Jena University Hospital, Department of Internal Medicine III, 07747 Jena, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Pfeil
authors.affiliation: Jena University Hospital, Department of Internal Medicine I, 07747 Jena, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Fritzenwanger
authors.affiliation: Jena University Hospital, Department of Internal Medicine I, 07747 Jena, Germany.
authors.forename: Hans R
authors.initials: HR
authors.lastname: Figulla
authors.affiliation: Jena University Hospital, Department of Internal Medicine I, 07747 Jena, Germany; Medical Faculty, Division of Cardiology, Pulmonology, and Vascular Medicine, University of Duesseldorf, 40225 Duesseldorf, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Jung
authors.affiliation: Jena University Hospital, Institute of Pathology, 07743 Jena, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Berndt
authors.affiliation: Jena University Hospital, Department of Internal Medicine I, 07747 Jena, Germany.
authors.forename: Marcus
authors.identifier: 0000-0001-6543-4684
authors.initials: M
authors.lastname: Franz
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D002311:Cardiomyopathy, Dilated / Q000097:blood* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D005353:Fibronectins / Q000097:blood*; D006333:Heart Failure / Q000097:blood* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000097:blood* / Q000473:pathology; D013318:Stroke Volume; D020257:Ventricular Remodeling
chemical_list: D015415:Biomarkers; D005353:Fibronectins; C000593306:fibronectinEDA, human