title: Bioactive new metabolites from the green alga Udotea orientalis growing on the Gorgonian coral Pseudopterogorgia rigida.
abstract: As part of our continued search for bioactive secondary metabolites from marine sources using a bioassay-guided fractionation technique (Cytotoxic and anti-trypanosome activities), we have examined the organic extract of Papua New Guinean collection of the green alga Udotea orientalis growing on the Gorgonian coral Pseudopterogorgia rigida. Successive HPLC investigations resulted in isolation of three new compounds, (+) curcuepoxide A, (+) curcuepoxide B and (+)-10α-hydroxycurcudiol. Analysis of different spectroscopic data e.g. UV, IR, LRMS, HRMS, 1D NMR and 2D NMR on the isolated compounds allowed for construction of the planar structures. Stereochemistry assignment at C-7 and C-10 in the new compounds was discussed. Isolated compounds were found to be active in an in vitro assay of antitrypanosome activity. The isolated compounds were found to have variable cytotoxic activity in human lung cancer cell lines.
authors.affiliation: a College of Pharmacy , Oregon State University , Corvallis , OR , USA.|b Faculty of Pharmacy , Cairo University , Cairo , Egypt.
authors.forename: Omar M M
authors.initials: OM
authors.lastname: Sabry
authors.affiliation: a College of Pharmacy , Oregon State University , Corvallis , OR , USA.
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Goeger
authors.affiliation: a College of Pharmacy , Oregon State University , Corvallis , OR , USA.|c Center for Marine Biotechnology and Biomedicine , University of California , San Diego , CA , USA.
authors.forename: William H
authors.initials: WH
authors.lastname: Gerwick
mesh_terms: D000818:Animals; D037421:Anthozoa; D000970:Antineoplastic Agents / Q000032:analysis / Q000302:isolation & purification*; D045744:Cell Line, Tumor; D000460:Chlorophyta / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology; D008968:Molecular Conformation; D013057:Spectrum Analysis; D013237:Stereoisomerism; D014344:Trypanocidal Agents / Q000032:analysis / Q000302:isolation & purification*; D014345:Trypanosoma / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D014344:Trypanocidal Agents
keywords: Pseudopterogorgia rigida; Udotea orientalis; antitrypanosome; lung cancer