title: Characterization of the mechanism of prolonged adaptation to osmotic stress of Jeotgalibacillus malaysiensis via genome and transcriptome sequencing analyses.
abstract: Jeotgalibacillus malaysiensis, a moderate halophilic bacterium isolated from a pelagic area, can endure higher concentrations of sodium chloride (NaCl) than other Jeotgalibacillus type strains. In this study, we therefore chose to sequence and assemble the entire J. malaysiensis genome. This is the first report to provide a detailed analysis of the genomic features of J. malaysiensis, and to perform genetic comparisons between this microorganism and other halophiles. J. malaysiensis encodes a native megaplasmid (pJeoMA), which is greater than 600 kilobases in size, that is absent from other sequenced species of Jeotgalibacillus. Subsequently, RNA-Seq-based transcriptome analysis was utilised to examine adaptations of J. malaysiensis to osmotic stress. Specifically, the eggNOG (evolutionary genealogy of genes: Non-supervised Orthologous Groups) and KEGG (Kyoto Encyclopaedia of Genes and Genomes) databases were used to elucidate the overall effects of osmotic stress on the organism. Generally, saline stress significantly affected carbohydrate, energy, and amino acid metabolism, as well as fatty acid biosynthesis. Our findings also indicate that J. malaysiensis adopted a combination of approaches, including the uptake or synthesis of osmoprotectants, for surviving salt stress. Among these, proline synthesis appeared to be the preferred method for withstanding prolonged osmotic stress in J. malaysiensis.
authors.affiliation: Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering, 81300 Skudai, Johor, Malaysia.
authors.forename: Amira Suriaty
authors.initials: AS
authors.lastname: Yaakop
authors.affiliation: University of Malaya, Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, 50603 Kuala Lumpur, Malaysia.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
authors.affiliation: University of Malaya, Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, 50603 Kuala Lumpur, Malaysia.
authors.forename: Robson
authors.initials: R
authors.lastname: Ee
authors.affiliation: University of Malaya, Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, 50603 Kuala Lumpur, Malaysia.
authors.forename: Yan Lue
authors.initials: YL
authors.lastname: Lim
authors.affiliation: University of Malaya, Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, 50603 Kuala Lumpur, Malaysia.
authors.forename: Siew-Kim
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering, 81300 Skudai, Johor, Malaysia.
authors.forename: Fazilah Abd
authors.initials: FA
authors.lastname: Manan
authors.affiliation: Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering, 81300 Skudai, Johor, Malaysia.
authors.forename: Kian Mau
authors.initials: KM
authors.lastname: Goh
mesh_terms: D000220:Adaptation, Biological; D016000:Cluster Analysis; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D007473:Ion Channels / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D009997:Osmotic Pressure; D010802:Phylogeny; D058641:Planococcaceae / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D007473:Ion Channels