title: Synergistic Effect of Mesoporous Silica and Hydroxyapatite in Loaded Poly(DL-lactic-co-glycolic acid) Microspheres on the Regeneration of Bone Defects.
abstract: A microsphere composite made of poly(DL-lactic-co-glycolic acid) (PLGA), mesoporous silica nanoparticle (MSN), and nanohydroxyapatite (nHA) (PLGA-MSN/nHA) was prepared and evaluated as bone tissue engineering materials. The objective of this study was to investigate the synergistic effect of MSN/nHA on biocompatibility as well as its potential ability for bone formation. First, we found that this PLGA-MSN/nHA composite performed good characteristics on microstructure, mechanical strength, and wettability. By cell culture experiments, the adhesion and proliferation rate of the cells seeded on PLGA-MSN/nHA composite was higher than those of the controls and high levels of osteogenetic factors such as ALP and Runx-2 were detected by reverse transcriptase polymerase chain reaction. Finally, this PLGA-MSN/nHA composite was implanted into the femur bone defect in a rabbit model, and its ability to induce bone regeneration was observed by histological examinations. Twelve weeks after implantation, the bone defects had significantly more formation of mature bone and less residual materials than in the controls. These results demonstrate that this PLGA-MSN/nHA composite, introducing both MSN and nHA into PLGA microspheres, can improve the biocompatibility and osteoinductivity of composite in vitro and in vivo and had potential application in bone regeneration.
authors.affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Shu
authors.identifier: 0000-0002-5696-1578
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China; Department of Orthopaedics, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, China.
authors.forename: Kai-Feng
authors.initials: KF
authors.lastname: Lin
authors.affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Fan
authors.affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Hu
authors.affiliation: Department of Orthopaedics, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yi-Nan
authors.initials: YN
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhong-Shang
authors.initials: ZS
authors.lastname: Guo
authors.affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Long
authors.identifier: 0000-0002-9854-9338
authors.initials: L
authors.lastname: Bi
authors.affiliation: Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jian
authors.identifier: 0000-0002-7245-0671
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects*; D017886:Durapatite / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000627:therapeutic use; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry / Q000627:therapeutic use; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D012822:Silicon Dioxide / Q000737:chemistry / Q000627:therapeutic use; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D012822:Silicon Dioxide; D017886:Durapatite