title: Wen-Luo-Tong Prevents Glial Activation and Nociceptive Sensitization in a Rat Model of Oxaliplatin-Induced Neuropathic Pain.
abstract: One of the main dose-limiting complications of the chemotherapeutic agent oxaliplatin (OXL) is painful neuropathy. Glial activation and nociceptive sensitization may be responsible for the mechanism of neuropathic pain. The Traditional Chinese Medicine (TCM) Wen-luo-tong (WLT) has been widely used in China to treat chemotherapy induced neuropathic pain. However, there is no study on the effects of WLT on spinal glial activation induced by OXL. In this study, a rat model of OXL-induced chronic neuropathic pain was established and WLT was administrated. Pain behavioral tests and morphometric examination of dorsal root ganglia (DRG) were conducted. Glial fibrillary acidic protein (GFAP) immunostaining was performed, glial activation was evaluated, and the excitatory neurotransmitter substance P (SP) and glial-derived proinflammatory cytokine tumor necrosis factor-α (TNF-α) were analyzed. WLT treatment alleviated OXL-induced mechanical allodynia and mechanical hyperalgesia. Changes in the somatic, nuclear, and nucleolar areas of neurons in DRG were prevented. In the spinal dorsal horn, hypertrophy and activation of GFAP-positive astrocytes were averted, and the level of GFAP mRNA decreased significantly. Additionally, TNF-α mRNA and protein levels decreased. Collectively, these results indicate that WLT reversed both glial activation in the spinal dorsal horn and nociceptive sensitization during OXL-induced chronic neuropathic pain in rats.
authors.affiliation: Department of Oncology of Integrative Chinese and Western Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Bo
authors.identifier: 0000-0001-7170-701X
authors.initials: B
authors.lastname: Deng
authors.affiliation: Department of Oncology of Integrative Chinese and Western Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Liqun
authors.identifier: 0000-0002-0370-2126
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of Oncology of Integrative Chinese and Western Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Oncology of Integrative Chinese and Western Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Aiping
authors.identifier: 0000-0003-3812-6744
authors.initials: A
authors.lastname: Song
authors.affiliation: School of Clinical Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Yuanyuan
authors.identifier: 0000-0002-1463-7235
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Oncology of Integrative Chinese and Western Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Huangying
authors.identifier: 0000-0002-6165-5196
authors.initials: H
authors.lastname: Tan
authors.affiliation: Department of Oncology of Integrative Chinese and Western Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Qing
authors.identifier: 0000-0002-7953-8246
authors.initials: Q
authors.lastname: Xiang
authors.affiliation: Department of Oncology of Integrative Chinese and Western Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Lili
authors.identifier: 0000-0002-3010-3727
authors.initials: L
authors.lastname: Yu
authors.affiliation: School of Clinical Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Dandan
authors.identifier: 0000-0002-6223-4326
authors.initials: D
authors.lastname: Ke