title: Data generated from three quantitative mass spectral methods for the analysis of trivalent influenza vaccine antigens are compared.
abstract: Herein we present the data necessary for generation of alternative means to produce equimolar mixtures of peptides ("Design and Expression of a QconCAT Protein to Validate Hi3 Protein Quantification of Influenza Vaccine Antigens" (D.G.S. Smith, G. Gingras, Y. Aubin, T.D. Cyr, 2016) [1]), such as QConCAT ("Trends in QconCATs for targeted proteomics" (J. Chen, I.V. Turko, 2014) [2] , "Natural flanking sequences for peptides included in a quantification concatamer internal standard" (C.S. Cheung, K.W. Anderson, M. Wang, I.V. Turko, 2015) [3]) and SpikeTides versus the label free Hi3 approach. The experimental design and the interpretation of results are discussed in the original article [1].
authors.affiliation: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directorate, Health Products and Food Branch, Health Canada, Ottawa, ON, Canada.
authors.forename: Daryl G S
authors.initials: DG
authors.lastname: Smith
authors.affiliation: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directorate, Health Products and Food Branch, Health Canada, Ottawa, ON, Canada.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Gingras
authors.affiliation: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directorate, Health Products and Food Branch, Health Canada, Ottawa, ON, Canada.
authors.forename: Yves
authors.initials: Y
authors.lastname: Aubin
authors.affiliation: Centre for Biologics Evaluation, Biologics and Genetic Therapies Directorate, Health Products and Food Branch, Health Canada, Ottawa, ON, Canada.
authors.forename: Terry D
authors.initials: TD
authors.lastname: Cyr
keywords: Hemagglutinin; Hi3; Influenza; Optimization; Protein quantitation; QConCAT