title: Enhanced butanol production by solvent tolerance Clostridium acetobutylicum SE25 from cassava flour in a fibrous bed bioreactor.
abstract: To enhance the butanol productivity and reduce the material cost, acetone, butanol, and ethanol fermentation by Clostridium acetobutylicum SE25 was investigated using batch, repeated-batch and continuous cultures in a fibrous bed bioreactor, where cassava flour was used as the substrate. With periodical nutrient supplementation, stable butanol production was maintained for about 360h in a 6-cycle repeated-batch fermentation with an average butanol productivity of 0.28g/L/h and butanol yield of 0.32g/g-starch. In addition, the highest butanol productivity of 0.63g/L/h and butanol yield of 0.36g/g-starch were achieved when the dilution rate were investigated in continuous production of acetone, butanol, and ethanol using a fibrous bed bioreactor, which were 231.6% and 28.6% higher than those of the free-cell fermentation. On the other hand, this study also successfully comfirmed that the biofilm can provide an effective protection for the microbial cells which are growing in stressful environment.
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045, China.
authors.forename: Han-Guang
authors.initials: HG
authors.lastname: Li
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045, China.
authors.forename: Xing-Xing
authors.initials: XX
authors.lastname: Ma
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045, China. Electronic address: zqh_net@163.com.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045, China.
authors.forename: Ya-Qing
authors.initials: YQ
authors.lastname: Wu
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045, China.
authors.forename: Xun-Hang
authors.initials: XH
authors.lastname: Li
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology*; D000440:Butanols / Q000138:chemical synthesis*; D046969:Clostridium acetobutylicum / Q000378:metabolism*; D005285:Fermentation; D005433:Flour / Q000032:analysis; D002365:Manihot / Q000737:chemistry*; D012997:Solvents
chemical_list: D000440:Butanols; D012997:Solvents
keywords: Acetone-butanol-ethanol fermentation; Biofilm; Cassava flour; Clostridium acetobutylicum; Immobilization