title: What information is contained in the fluorescence correlation spectroscopy curves, and where.
abstract: We discuss the application of fluorescence correlation spectroscopy (FCS) for characterization of anomalous diffusion of tracer particles in crowded environments. While the fact of anomaly may be detected by the standard fitting procedure, the value of the exponent α of anomalous diffusion may be not reproduced correctly for non-Gaussian anomalous diffusion processes. The important information is however contained in the asymptotic behavior of the fluorescence autocorrelation function at long and at short times. Thus, analysis of the short-time behavior gives reliable values of α and of lower moments of the distribution of particles' displacement, which allows us to confirm or reject its Gaussian nature. The method proposed was tested on the FCS data obtained in artificial crowded fluids and in living cells.
authors.affiliation: Institute of Physics, Humboldt University Berlin, Newtonstrasse 15, D-12489 Berlin, Germany.|School of Analytical Sciences Adlershof (SALSA), Albert-Einstein-Strasse 5-9, D-12489 Berlin, Germany.
authors.forename: S M J
authors.initials: SM
authors.lastname: Khadem
authors.affiliation: Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Strasse 24-25, D-14476 Potsdam-Golm, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Hille
authors.affiliation: School of Analytical Sciences Adlershof (SALSA), Albert-Einstein-Strasse 5-9, D-12489 Berlin, Germany.|Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Strasse 24-25, D-14476 Potsdam-Golm, Germany.
authors.forename: H-G
authors.initials: HG
authors.lastname: Löhmannsröben
authors.affiliation: Institute of Physics, Humboldt University Berlin, Newtonstrasse 15, D-12489 Berlin, Germany.|School of Analytical Sciences Adlershof (SALSA), Albert-Einstein-Strasse 5-9, D-12489 Berlin, Germany.
authors.forename: I M
authors.initials: IM
authors.lastname: Sokolov