title: MicroRNA-127 is a tumor suppressor in human esophageal squamous cell carcinoma through the regulation of oncogene FMNL3.
abstract: In this study, we investigated the expression patterns and functional roles of microRNA 127 (miR-127) and its target gene Formin-Like 3 (FMNL3) in human esophageal squamous cell carcinoma (ESCC). Quantitative RT-PCR (qRT-PCR) was used to compare miR-127 expression between ESCC cell lines and normal esophageal epithelium cell line, as well as paired ESCC tumors and adjacent normal esophageal tissues in 33 patients. We found miR-127 was aberrantly downregulated in both ESCC cell lines and human ESCC tumors. In ESCC cell lines TE-1 and ECA109 cells, lentiviral-induced miR-127 upregulation markedly inhibited cancer proliferation and migration in vitro, and tumorigenicity in vivo. Through dual-luciferase assay and qRT-PCR, FMNL3 was confirmed to be the downstream target gene of miR-127 in ESCC. Finally, FMNL3 was downregulated by siRNA in TE-1 and ECA109 cells. And we discovered that SiRNA-induced FMNL3 downregulation had tumor suppressive effect in ESCC, inhibiting cancer proliferation, migration in vitro, and tumorigenicity in vivo. These results suggest that miR-127 is downregulated and acting as tumor suppressor in ESCC. Inversely, FMNL3, the target gene of miR-127, is upregulated and acting as an oncogene in ESCC.
authors.affiliation: Department of Cardiothoracic Surgery, Wuhan General Hospital of Guangzhou Command, Wuhan 430000, China; Department of Cardiothoracic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xuhui
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of anesthesiology, The Third Affiliated Hospital, Southern Medical University, Guangzhou 510000, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Cardiothoracic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Kaican
authors.initials: K
authors.lastname: Cai
authors.affiliation: Department of Cardiothoracic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Wujun
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Cardiothoracic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Ju
authors.affiliation: Department of Cardiothoracic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xiyao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Cardiothoracic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Haofei
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Cardiothoracic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: wuh772@aol.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D004947:Esophagus / Q000166:cytology / Q000473:pathology; D005260:Female; D000080865:Formins; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009857:Oncogenes / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C476209:FMNL3 protein, human; D000080865:Formins; C547276:MIRN127 microRNA, human; D035683:MicroRNAs; D011506:Proteins
keywords: Cancer in vitro growth; Cancer in vivo growth, FMNL3; Esophageal squamous cell carcinoma; MiR-127