title: In silico data analyses of recombinases GdDMC1A and GdDMC1B from Giardia duodenalis.
abstract: Giardia duodenalis is a worldwide protozoa known causing diarrhea in all vertebrates, humans among these. Homologous recombination is a mechanism that provides genomic stability. Two putative recombinases were identified in G. duodenalis genome: GdDMC1A and GdDMC1B. In this article, we describe the identification of conserved domains in GdDMC1A and GdDMC1B, such as: DNA binding domains (Helix-turn-helix motif, loops 1 and 2) and an ATPcap and Walker A and B motifs associated with ATP binding and hydrolysis, phylogenetic analyses among assemblages and three-dimensional structure modeling of these recombinases using bioinformatics tools. Also, experimental data is described about LD50 determination for ionizing radiation in trophozoites of G. duodenalis. Additionally, as recombinases, GdDMC1A and GdDMC1B were used to rescue a defective Saccharomyces cerevisiae Δ rad51 strain under genotoxic conditions and data is described. The data described here are related to the research article entitled "Characterization of recombinase DMC1B and its functional role as Rad51 in DNA damage repair in Giardia duodenalis trophozoites" (Torres-Huerta et al.,) [1].
authors.affiliation: Genetics and Molecular Biology Department, Centro de Investigación y de Estudios Avanzados del IPN, Av. Instituto Politécnico Nacional No. 2508, C.P. 07360 México D.F., Mexico.
authors.forename: Ana Laura
authors.initials: AL
authors.lastname: Torres-Huerta
authors.affiliation: Genetics and Molecular Biology Department, Centro de Investigación y de Estudios Avanzados del IPN, Av. Instituto Politécnico Nacional No. 2508, C.P. 07360 México D.F., Mexico.
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Martínez-Miguel
authors.affiliation: Genetics and Molecular Biology Department, Centro de Investigación y de Estudios Avanzados del IPN, Av. Instituto Politécnico Nacional No. 2508, C.P. 07360 México D.F., Mexico.
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Bazán-Tejeda
authors.affiliation: Genetics and Molecular Biology Department, Centro de Investigación y de Estudios Avanzados del IPN, Av. Instituto Politécnico Nacional No. 2508, C.P. 07360 México D.F., Mexico.
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Bermúdez-Cruz
keywords: 3D structure prediction; DL50 for ionizing radiation; DMC1B and DMC1A; In silico analyses