title: Microbial Community Dynamics and Response to Plant Growth-Promoting Microorganisms in the Rhizosphere of Four Common Food Crops Cultivated in Hydroponics.
abstract: Plant growth promoting microorganisms (PGPMs) of the plant root zone microbiome have received limited attention in hydroponic cultivation systems. In the framework of a project aimed at the development of a biological life support system for manned missions in space, we investigated the effects of PGPMs on four common food crops (durum and bread wheat, potato and soybean) cultivated in recirculating hydroponic systems for a whole life cycle. Each crop was inoculated with a commercial PGPM mixture and the composition of the microbial communities associated with their root rhizosphere, rhizoplane/endosphere and with the recirculating nutrient solution was characterised through 16S- and ITS-targeted Illumina MiSeq sequencing. PGPM addition was shown to induce changes in the composition of these communities, though these changes varied both between crops and over time. Microbial communities of PGPM-treated plants were shown to be more stable over time. Though additional development is required, this study highlights the potential benefits that PGPMs may confer to plants grown in hydroponic systems, particularly when cultivated in extreme environments such as space.
authors.affiliation: In Vitro Biology and Horticulture, Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, 653 Coupure Links, 9000, Ghent, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Sheridan
authors.affiliation: In Vitro Biology and Horticulture, Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, 653 Coupure Links, 9000, Ghent, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Depuydt
authors.affiliation: In Vitro Biology and Horticulture, Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, 653 Coupure Links, 9000, Ghent, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: De Ro
authors.affiliation: In Vitro Biology and Horticulture, Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, 653 Coupure Links, 9000, Ghent, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Petit
authors.affiliation: In Vitro Biology and Horticulture, Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, 653 Coupure Links, 9000, Ghent, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Van Gysegem
authors.affiliation: In Vitro Biology and Horticulture, Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, 653 Coupure Links, 9000, Ghent, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Delaere
authors.affiliation: Controlled Environment Systems Research Facility, School of Environmental Sciences, University of Guelph, Guelph, ON, NIG 2W1, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Dixon
authors.affiliation: Controlled Environment Systems Research Facility, School of Environmental Sciences, University of Guelph, Guelph, ON, NIG 2W1, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Stasiak
authors.affiliation: Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland.
authors.forename: S B
authors.initials: SB
authors.lastname: Aciksöz
authors.affiliation: Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Frossard
authors.affiliation: Division of Plant Biology and Crop Science, Department of Agricultural Sciences, University of Naples Federico II Naples, Via Università, 100 80055, Portici, Naples, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Paradiso
authors.affiliation: Division of Plant Biology and Crop Science, Department of Agricultural Sciences, University of Naples Federico II Naples, Via Università, 100 80055, Portici, Naples, Italy.
authors.forename: S
authors.initials: S
authors.lastname: De Pascale
authors.affiliation: Division of Microbiology, Department of Agricultural Sciences, University of Naples Federico II Naples, Via Università, 100 80055, Portici, Naples, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Ventorino
authors.affiliation: Department of Mathematical Modelling, Statistics and Bioinformatics, Ghent University, Ghent, Belgium.
authors.forename: T
authors.initials: T
authors.lastname: De Meyer
authors.affiliation: Department of Food Quality and Food Safety, Ghent University, Coupure links 653, B-9000, Ghent, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Sas
authors.affiliation: In Vitro Biology and Horticulture, Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, 653 Coupure Links, 9000, Ghent, Belgium. danny.geelen@ugent.be.
authors.forename: D
authors.identifier: 0000-0001-8105-3937
authors.initials: D
authors.lastname: Geelen
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics; D001483:Base Sequence; D044822:Biodiversity; D018556:Crops, Agricultural / Q000254:growth & development* / Q000382:microbiology*; D004269:DNA, Bacterial; D004271:DNA, Fungal; D005502:Food; D005658:Fungi / Q000145:classification / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D018527:Hydroponics; D008018:Life Cycle Stages; D059013:Microbial Consortia / Q000235:genetics; D010802:Phylogeny; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D011198:Solanum tuberosum / Q000254:growth & development / Q000382:microbiology; D013025:Glycine max / Q000254:growth & development / Q000382:microbiology; D014908:Triticum / Q000254:growth & development / Q000382:microbiology; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004271:DNA, Fungal; D012336:RNA, Ribosomal, 16S
keywords: Dynamics; Hydroponics; Plant; Plant growth-promoting organisms; Rhizosphere; Space