title: In vitro and long-term (2-year follow-up) in vivo osteogenic activities of human periosteum-derived osteoblasts seeded into growth factor-releasing polycaprolactone/pluronic F127 beads scaffolds.
abstract: Polycaprolactone (PCL) is a biodegradable polyester that is bioresorbable and biocompatible, and is widely used in medical fields. This study examines in vitro and in vivo osteogenic activities of cultured human periosteum-derived osteoblasts (POs) seeded into growth factor (bone morphogenic protein 2 [BMP-2] or vascular endothelial growth factor [VEGF])-releasing scaffolds of PCL beads coated with Pluronic F127. Each growth factor immobilized in the PCL/F127 is cumulatively released from the beads for more than 40 days (up to 3.04 ± 0.08 ng mg-1BMP-2 and 3.41 ± 0.18 ng mg-1VEGF in 42 days). Long-term (∼2 years) experimental results obtained in a miniature pig model suggest that POs seeded into BMP-2 + VEGF-releasing PCL/P-F127 beads are the most effective for bone repair. In in vitro assays, osteogenic activities were higher in POs seeded into BMP-2-releasing PCL/Pluronic F127 beads at earlier time points and in POs seeded into BMP-2 + VEGF-releasing PCL/Pluronic F127 beads at later time points. These results suggest that the combination of BMP-2 and VEGF more sufficiently stimulates (in particular at late time points) osteoblast differentiation of POs seeded in the PCL/F127 in vitro and in vivo, and thus allows effective bone regeneration. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 363-376, 2017.
authors.affiliation: Department of Advanced Materials, Hannam University, Daejeon, 305-811, Republic of Korea.
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju, 660-702, Republic of Korea.
authors.forename: Dong Kyun
authors.initials: DK
authors.lastname: Woo
authors.affiliation: Department of Advanced Materials, Hannam University, Daejeon, 305-811, Republic of Korea.
authors.forename: Tae Ho
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Advanced Materials, Hannam University, Daejeon, 305-811, Republic of Korea.|R&D Center, Metabiomed Co. Ltd, Osong-eup, Cheongju, 363-951, Republic of Korea.
authors.forename: Jun Goo
authors.initials: JG
authors.lastname: Kang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital Institute of Health Sciences Gyeongsang National University, Jinju, 660-702, Republic of Korea.
authors.forename: Jeong-Won
authors.initials: JW
authors.lastname: Yun
authors.affiliation: Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital Institute of Health Sciences Gyeongsang National University, Jinju, 660-702, Republic of Korea.
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital Institute of Health Sciences Gyeongsang National University, Jinju, 660-702, Republic of Korea.
authors.forename: Bong-Wook
authors.initials: BW
authors.lastname: Park
authors.affiliation: Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital Institute of Health Sciences Gyeongsang National University, Jinju, 660-702, Republic of Korea.
authors.forename: Young-Hoon
authors.initials: YH
authors.lastname: Kang
authors.affiliation: Department of Theriogenology and Biotechnology College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Gyu-Jin
authors.initials: GJ
authors.lastname: Rho
authors.affiliation: Department of Theriogenology and Biotechnology College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Si-Jung
authors.initials: SJ
authors.lastname: Jang
authors.affiliation: Department of Theriogenology and Biotechnology College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Ji-Sung
authors.initials: JS
authors.lastname: Park
authors.affiliation: Department of Veterinary Medical Imaging College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Hee-Chun
authors.initials: HC
authors.lastname: Lee
authors.affiliation: Department of Veterinary Medical Imaging College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Young Min
authors.initials: YM
authors.lastname: Yoon
authors.affiliation: Department of Veterinary Medical Imaging College of Veterinary Medicine, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Hwang
authors.affiliation: Department of Biochemistry, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju, 660-702, Republic of Korea.
authors.forename: Deok Ryong
authors.initials: DR
authors.lastname: Kim
authors.affiliation: Department of Orthopaedic Surgery, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju, 660-702, Republic of Korea.
authors.forename: Sun-Chul
authors.initials: SC
authors.lastname: Hwang
authors.affiliation: Department of Anatomy, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju, 660-702, Republic of Korea.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Department of Nanobiomedical Science, Dankook University, Cheonan, 330-714, Republic of Korea.
authors.forename: Ho Young
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Department of Nanobiomedical Science, Dankook University, Cheonan, 330-714, Republic of Korea.
authors.forename: Se Heang
authors.initials: SH
authors.lastname: Oh
authors.affiliation: Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital Institute of Health Sciences Gyeongsang National University, Jinju, 660-702, Republic of Korea.
authors.forename: June-Ho
authors.initials: JH
authors.lastname: Byun
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D010521:Periosteum / Q000166:cytology / Q000378:metabolism*; D020442:Poloxamer / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D013552:Swine; D013556:Swine, Miniature; D054457:Tissue Scaffolds / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry*
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D011091:Polyesters; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D020442:Poloxamer; C016240:polycaprolactone
keywords: BMP-2; PCL/pluronic F127 beads; VEGF; periosteum-derived osteoblasts