title: Pectin mediated gold nanoparticles induces apoptosis in mammary adenocarcinoma cell lines.
abstract: Pectin and its several modified forms have shown remarkable impact in therapeutic use against various cancers. In the present study, pectin, an anionic polysaccharide isolated from Musa paradisiaca is employed for the synthesis of gold nanoparticles at ambient temperature conditions. The synthesized nanoparticles were characterized using microscopic and spectroscopic studies and its anti-cancer potential was evaluated in mammary adenocarcinoma cell lines MCF-7 and MDA-MB-231. Apoptosis induction was evident from increase in sub-G1 population studied using flow cytometry analysis. DNA damage followed by cell death in pectin mediated gold nanoparticles (p-GNPs) treated cells was confirmed by Comet assay. Uptake of p-GNPs by cancer cells (MCF-7 and MDA-MB-231) was analyzed using FE-SEM which revealed the presence of p-GNPs as aggregates over the surface of cells with loss in cellular integrity compared to control cells.
authors.affiliation: Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
authors.forename: K S Uma
authors.initials: KS
authors.lastname: Suganya
authors.affiliation: Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600 119, India. Electronic address: govindaraju@sathyabamauniversity.ac.in.
authors.forename: K
authors.initials: K
authors.lastname: Govindaraju
authors.affiliation: Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
authors.forename: V Ganesh
authors.initials: VG
authors.lastname: Kumar
authors.affiliation: Nanoscience Division, Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
authors.forename: V
authors.initials: V
authors.lastname: Karthick
authors.affiliation: Centre for Drug Discovery and Development, Sathyabama University, Chennai 600 119, India.
authors.forename: Krupakar
authors.initials: K
authors.lastname: Parthasarathy
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy*; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006046:Gold / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010368:Pectins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000970:Antineoplastic Agents; D006046:Gold; D010368:Pectins
keywords: Anti-cancer; Apoptosis; Flow cytometry; Gold nanoparticles; Pectin