title: Transcriptional profiling of the effect of lipopolysaccharide (LPS) pretreatment in blood from probiotics-treated dairy cows.
abstract: Probiotic supplements are beneficial for animal health and rumen function; and lipopolysaccharides (LPS) from gram negative bacteria have been associated with inflammatory diseases. In this study the transcriptional profile in whole blood collected from probiotics-treated cows was investigated in response to stimulation with lipopolysaccharides (LPS) in vitro. Microarray experiment was performed between LPS-treated and control samples using the Agilent one-color bovine v2 bovine (v2) 4x44K array slides. Global gene expression analysis identified 13,658 differentially expressed genes (fold change cutoff ≥ 2, P < 0.05), 3816 upregulated genes and 9842 downregulated genes in blood in response to LPS. Treatment with LPS resulted in increased expression of TLR4 (Fold change (FC) = 3.16) and transcription factor NFkB (FC = 5.4) and decreased the expression of genes including TLR1 (FC = - 2.54), TLR3 (FC = - 2.43), TLR10 (FC = - 3.88), NOD2 (FC = - 2.4), NOD1 (FC = - 2.45) and pro-inflammatory cytokine IL1B (- 3.27). The regulation of the genes involved in inflammation signaling pathway suggests that probiotics may stimulate the innate immune response of animal against parasitic and bacterial infections. We have provided a detailed description of the experimental design, microarray experiment and normalization and analysis of data which have been deposited into NCBI Gene Expression Omnibus (GEO): GSE75240.
authors.affiliation: Department of Energy and Environmental Systems, North Carolina A&T State University, Greensboro, NC, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Adjei-Fremah
authors.affiliation: Department of Energy and Environmental Systems, North Carolina A&T State University, Greensboro, NC, USA.
authors.forename: Kingsley
authors.initials: K
authors.lastname: Ekwemalor
authors.affiliation: Department of Energy and Environmental Systems, North Carolina A&T State University, Greensboro, NC, USA.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Asiamah
authors.affiliation: Department of Animal Sciences, North Carolina A&T State University, Greensboro, NC, USA.
authors.forename: Hamid
authors.initials: H
authors.lastname: Ismail
authors.affiliation: Department of Animal Sciences, North Carolina A&T State University, Greensboro, NC, USA.
authors.forename: Mulumebet
authors.initials: M
authors.lastname: Worku