title: Chiral PCB 91 and 149 Toxicity Testing in Embryo and Larvae (Danio rerio): Application of Targeted Metabolomics via UPLC-MS/MS.
abstract: In this study, we aimed to investigate the dysfunction of zebrafish embryos and larvae induced by rac-/(+)-/(-)- PCB91 and rac-/(-)-/(+)- PCB149. UPLC-MS/MS (Ultra-performance liquid chromatography coupled with mass spectrometry) was employed to perform targeted metabolomics analysis, including the quantification of 22 amino acids and the semi-quantitation of 22 other metabolites. Stereoselective changes in target metabolites were observed in embryos and larvae after exposure to chiral PCB91 and PCB149, respectively. In addition, statistical analyses, including PCA and PLS-DA, combined with targeted metabolomics were conducted to identify the characteristic metabolites and the affected pathways. Most of the unique metabolites in embryos and larvae after PCB91/149 exposure were amino acids, and the affected pathways for zebrafish in the developmental stage were metabolic pathways. The stereoselective effects of PCB91/149 on the metabolic pathways of zebrafish embryos and larvae suggest that chiral PCB91/149 exposure has stereoselective toxicity on the developmental stages of zebrafish.
authors.affiliation: Institute of Quality Standards &Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.|College of Science, China Agricultural University, Beijing 100193, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Chai
authors.affiliation: College of Science, China Agricultural University, Beijing 100193, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Cui
authors.affiliation: Institute of Quality Standards &Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yin
authors.affiliation: College of Science, China Agricultural University, Beijing 100193, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Quality Standards &Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qiu
authors.affiliation: College of Science, China Agricultural University, Beijing 100193, China.
authors.forename: Chengju
authors.initials: C
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D055432:Metabolomics; D015999:Multivariate Analysis; D011078:Polychlorinated Biphenyls / Q000737:chemistry / Q000633:toxicity*; D025341:Principal Component Analysis; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry; D018675:Toxicity Tests; D015027:Zebrafish / Q000196:embryology*
chemical_list: D000596:Amino Acids; C120129:2,2',3,4',5',6-hexachlorobiphenyl; D011078:Polychlorinated Biphenyls; C000627370:2,2',3,4',6-pentachlorobiphenyl