title: 3D perfusion bioreactor-activated porous granules on implant fixation and early bone formation in sheep.
abstract: Early fixation of total joint arthroplasties is crucial for ensuring implant survival. An alternative bone graft material in revision surgery is needed to replace the current gold standard, allograft, seeing that the latter is associated with several disadvantages. The incubation of such a construct in a perfusion bioreactor has been shown to produce viable bone graft materials. This study aimed at producing larger amounts of viable bone graft material (hydroxyapatite 70% and β-tricalcium-phosphate 30%) in a novel perfusion bioreactor. The abilities of the bioreactor-activated graft material to induce early implant fixation were tested in a bilateral implant defect model in sheep, with allograft as the control group. Defects were bilaterally created in the distal femurs of the animals, and titanium implants were inserted. The concentric gaps around the implants were randomly filled with either allograft, granules, granules with bone marrow aspirate or bioreactor-activated graft material. Following an observation time of 6 weeks, early implant fixation and bone formation were assessed by micro-CT scanning, mechanical testing, and histomorphometry. Bone formations were seen in all groups, while no significant differences between groups were found regarding early implant fixation. The microarchitecture of the bone formed by the synthetic graft materials resembled that of allograft. Histomorphometry revealed that allograft induced significantly more bone and less fibrous tissue (p < 0.05). In conclusion, bone formation was observed in all groups, while the bioreactor-activated graft material did not reveal additional effects on early implant fixation comparable to allograft in this model. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2465-2476, 2017.
authors.affiliation: Orthopaedic Research Laboratory, Department of Orthopaedic Surgery and Traumatology, Odense University Hospital, Institute of Clinical Research, University of Southern Denmark, J.B. Winsloewsvej 15, 3rd floor, DK-5000 Odense C, Denmark.
authors.forename: Ming
authors.initials: M
authors.lastname: Ding
authors.affiliation: Orthopaedic Research Laboratory, Department of Orthopaedic Surgery and Traumatology, Odense University Hospital, Institute of Clinical Research, University of Southern Denmark, J.B. Winsloewsvej 15, 3rd floor, DK-5000 Odense C, Denmark.
authors.forename: Susan S
authors.initials: SS
authors.lastname: Henriksen
authors.affiliation: Fin-Ceramica Faenza, S.p.A, Faenza, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Martinetti
authors.affiliation: Orthopaedic Research Laboratory, Department of Orthopaedic Surgery and Traumatology, Odense University Hospital, Institute of Clinical Research, University of Southern Denmark, J.B. Winsloewsvej 15, 3rd floor, DK-5000 Odense C, Denmark.
authors.forename: Søren
authors.initials: S
authors.lastname: Overgaard
mesh_terms: D000818:Animals; D019149:Bioreactors; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D005269:Femur / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D020007:Implants, Experimental; D010012:Osteogenesis; D016062:Porosity; D012756:Sheep
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D017886:Durapatite
keywords: 3D perfusion bioreactor; histomorphometry; implant fixation; microarchitecture; porous granules