title: Designing Synthetic Flexible Gene Regulation Networks Using RNA Devices in Cyanobacteria.
abstract: In recent years, studies on the development of gene regulation tools in cyanobacteria have been extensively conducted toward efficient production of valuable chemicals. However, there is considerable scope for improving the economic feasibility of production. To improve a recently reported gene induction system using anhydrotetracycline (aTc)-TetR and an endogenous gene repression system using small antisense RNA in the filamentous nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (Anabaena), we constructed a positive feedback loop, in which gfp and a small antisense RNA for tetR are controlled by an aTc-inducible promoter. GFP expression in this improved system was higher and longer than the system lacking tetR repression. In addition, by using TetR aptamer and a riboswitch, we succeeded in achieving a superior and longer induction of GFP expression even under high-light conditions. Hence, efficient gene induction systems were established in Anabaena by designing a gene regulation network using RNA-based tools.
authors.affiliation: Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology , Nagatsuta 4259-R1-8, Midori-ku, Yokohama 226-8503, Japan.|Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Tokyo 102-0075, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Higo
authors.affiliation: Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology , Nagatsuta 4259-R1-8, Midori-ku, Yokohama 226-8503, Japan.|Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Tokyo 102-0075, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Isu
authors.affiliation: Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology , Nagatsuta 4259-R1-8, Midori-ku, Yokohama 226-8503, Japan.|Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Tokyo 102-0075, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fukaya
authors.affiliation: Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology , Nagatsuta 4259-R1-8, Midori-ku, Yokohama 226-8503, Japan.|Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Tokyo 102-0075, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Hisabori
mesh_terms: D017033:Anabaena / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D053263:Gene Regulatory Networks; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D058928:Riboswitch / Q000235:genetics; D058615:Synthetic Biology; D013753:Tetracycline Resistance / Q000235:genetics; D013754:Tetracyclines / Q000494:pharmacology
chemical_list: D012329:RNA, Bacterial; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D058928:Riboswitch; D013754:Tetracyclines; D049452:Green Fluorescent Proteins; C016229:4-epianhydrotetracycline
keywords: cyanobacteria; gene regulation system; riboswitch; small RNA