title: AC-1001 H3 CDR peptide induces apoptosis and signs of autophagy in vitro and exhibits antimetastatic activity in a syngeneic melanoma model.
abstract: Antibody-derived peptides modulate functions of the immune system and are a source of anti-infective and antitumor substances. Recent studies have shown that they comprise amino acid sequences of immunoglobulin complementarity-determining regions, but also fragments of constant regions. VH CDR3 of murine mAb AC-1001 displays antimetastatic activities using B16F10-Nex2 murine melanoma cells in a syngeneic model. The peptide was cytotoxic in vitro in murine and human melanoma cells inducing reactive oxygen species (ROS) and apoptosis by the intrinsic pathway. Signs of autophagy were also suggested by the increased expression of LC3/LC3II and Beclin 1 and by ultrastructural evidence. AC-1001 H3 bound to both G- and F-actin and inhibited tumor cell migration. These results are important evidence of the antitumor activity of Ig CDR-derived peptides.
authors.affiliation: Unidade de Oncologia Experimental (UNONEX) Universidade Federal de São Paulo (UNIFESP) Brazil.
authors.forename: Aline N
authors.initials: AN
authors.lastname: Rabaça
authors.affiliation: Unidade de Oncologia Experimental (UNONEX) Universidade Federal de São Paulo (UNIFESP) Brazil; Núcleo Integrado de Biotecnologia Universidade de Mogi das Cruzes Brazil.
authors.forename: Denise C
authors.initials: DC
authors.lastname: Arruda
authors.affiliation: Unidade de Oncologia Experimental (UNONEX) Universidade Federal de São Paulo (UNIFESP) Brazil.
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Figueiredo
authors.affiliation: Unidade de Oncologia Experimental (UNONEX) Universidade Federal de São Paulo (UNIFESP) Brazil.
authors.forename: Mariana H
authors.initials: MH
authors.lastname: Massaoka
authors.affiliation: Unidade de Oncologia Experimental (UNONEX) Universidade Federal de São Paulo (UNIFESP) Brazil.
authors.forename: Camyla F
authors.initials: CF
authors.lastname: Farias
authors.affiliation: Departamento de Ciência e Tecnologia Universidade Federal de São Paulo (UNIFESP) São José dos Campos Brazil.
authors.forename: Dayane B
authors.initials: DB
authors.lastname: Tada
authors.affiliation: Recepta Biopharma São Paulo Brazil.
authors.forename: Vera C
authors.initials: VC
authors.lastname: Maia
authors.affiliation: Laboratório Especial de Toxinologia Aplicada Instituto Butantan São Paulo Brazil.
authors.forename: Pedro I
authors.initials: PI
authors.lastname: Silva Junior
authors.affiliation: Unidade de Oncologia Experimental (UNONEX) Universidade Federal de São Paulo (UNIFESP) Brazil.
authors.forename: Natalia
authors.initials: N
authors.lastname: Girola
authors.affiliation: Departamento de Parasitologia Universidade Federal de São Paulo (UNIFESP) Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Real
authors.affiliation: Departamento de Parasitologia Universidade Federal de São Paulo (UNIFESP) Brazil.
authors.forename: Renato A
authors.initials: RA
authors.lastname: Mortara
authors.affiliation: Microbiology and Virology Unit Department of Biomedical Biotechnological and Translational Sciences Universitá degli Studi di Parma Italy.
authors.forename: Luciano
authors.initials: L
authors.lastname: Polonelli
authors.affiliation: Unidade de Oncologia Experimental (UNONEX) Universidade Federal de São Paulo (UNIFESP) Brazil.
authors.forename: Luiz R
authors.initials: LR
authors.lastname: Travassos
keywords: apoptosis; autophagy; immunoglobulin CDR; melanoma; peptides