title: C30F12.4 influences oogenesis, fat metabolism, and lifespan in C. elegans.
abstract: Reproduction, fat metabolism, and longevity are intertwined regulatory axes; recent studies in C. elegans have provided evidence that these processes are directly coupled. However, the mechanisms by which they are coupled and the reproductive signals modulating fat metabolism and lifespan are poorly understood. Here, we find that an oogenesis-enriched gene, c30f12.4, is specifically expressed and located in germ cells and early embryos; when the gene is knocked out, oogenesis is disrupted and brood size is decreased. In addition to the reproductive phenotype, we find that the loss of c30f12.4 alters fat metabolism, resulting in decreased fat storage and smaller lipid droplets. Meanwhile, c30f12.4 mutant worms display a shortened lifespan. Our results highlight an important role for c30f12.4 in regulating reproduction, fat homeostasis, and aging in C. elegans, which helps us to better understand the relationship between these processes.
authors.affiliation: International Peace Maternity & Child Health Hospital, Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Institute of Embryo-Fetal Original Adult Disease, Shanghai Jiaotong University, Shanghai, 200030, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, 230026, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: International Peace Maternity & Child Health Hospital, Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Institute of Embryo-Fetal Original Adult Disease, Shanghai Jiaotong University, Shanghai, 200030, China.
authors.forename: Guishuan
authors.initials: G
authors.lastname: Wang
authors.affiliation: International Peace Maternity & Child Health Hospital, Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Institute of Embryo-Fetal Original Adult Disease, Shanghai Jiaotong University, Shanghai, 200030, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Wang
authors.affiliation: International Peace Maternity & Child Health Hospital, Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Institute of Embryo-Fetal Original Adult Disease, Shanghai Jiaotong University, Shanghai, 200030, China.
authors.forename: Ajuan
authors.initials: A
authors.lastname: Liang
authors.affiliation: International Peace Maternity & Child Health Hospital, Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Institute of Embryo-Fetal Original Adult Disease, Shanghai Jiaotong University, Shanghai, 200030, China. huanghefg@sjtu.edu.cn.
authors.forename: Hefeng
authors.initials: H
authors.lastname: Huang
authors.affiliation: International Peace Maternity & Child Health Hospital, Shanghai Key laboratory for Reproductive Medicine, School of Medicine, Institute of Embryo-Fetal Original Adult Disease, Shanghai Jiaotong University, Shanghai, 200030, China. sunfei@shsmu.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D066292:Lipid Droplets / Q000378:metabolism*; D050356:Lipid Metabolism / Q000502:physiology*; D008136:Longevity / Q000502:physiology*; D009154:Mutation; D009866:Oogenesis / Q000502:physiology*
chemical_list: D029742:Caenorhabditis elegans Proteins
keywords: C30F12.4; fat metabolism; lifespan; oogenesis