title: Preparation of K+-Doped Core-Shell NaYF4:Yb, Er Upconversion Nanoparticles and its Application for Fluorescence Immunochromatographic Assay of Human Procalcitonin.
abstract: In the present study, we reported a convenient route to prepare well dispersed and functionalized K+-doped core-shell upconversion nanoparticles (UCP) by layer-by-layer (LbL) assembly of polyelectrolytes. UCP was firstly transferred to aqueous phase using cationic surfactant cetyl trimethyl ammonium bromide (CTAB) via hydrophobic interaction without removing the existing oleic acid (OA). Then the positively charged hydrophilic UCP@CTAB was further alternately deposited with negatively charged [poly (sodium 4-styrenesulfonate)] (PSS), positively charged [poly (allylamine hydrochloride)] (PAH) and negatively charged [poly (acrylic acid)] (PAA). The final carboxyl functionalized UCP@CTAB@PSS@PAH@PAA was then conjugated with monoclonal antibody1 (AB1) of procalcitonin (PCT), resulting in successful detection of PCT antigens based on the immunochromatographic assay (ICA). Linear response was achieved from 0 to 10 ng/mL, and the lowest limit of detection (LLD) was 0.18 ng/mL.
authors.affiliation: College of Chemistry and Molecular Engineering, Nanjing Tech University, No.30 Puzhu Road(S), Nanjing, 211816, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.affiliation: College of Chemistry and Molecular Engineering, Nanjing Tech University, No.30 Puzhu Road(S), Nanjing, 211816, China.
authors.forename: Lijiang
authors.initials: L
authors.lastname: Lei
authors.affiliation: College of Chemistry and Molecular Engineering, Nanjing Tech University, No.30 Puzhu Road(S), Nanjing, 211816, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Feng
authors.affiliation: College of Chemistry and Molecular Engineering, Nanjing Tech University, No.30 Puzhu Road(S), Nanjing, 211816, China.
authors.forename: Hongman
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Molecular Engineering, Nanjing Tech University, No.30 Puzhu Road(S), Nanjing, 211816, China. hanyw@njtech.edu.cn.
authors.forename: Yuwang
authors.initials: Y
authors.lastname: Han
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D002116:Calcitonin / Q000097:blood* / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D004871:Erbium / Q000737:chemistry*; D005453:Fluorescence; D005459:Fluorides / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D015018:Ytterbium / Q000737:chemistry*; D015019:Yttrium / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D015019:Yttrium; D004871:Erbium; D002116:Calcitonin; D015018:Ytterbium; D005459:Fluorides
keywords: Core-shell; K+-doped; Layer-by-layer assembly; Procalcitonin; Upconversion