title: Use of Capillary Electrophoresis for Polysaccharide Studies and Applications.
abstract: CE applications to charged polysaccharides are briefly reported. A simple procedure is presented to determine the esterification degree of a hyaluronan derivative. In this case the degree of substitution was as low as 14 %.The molecular weight distribution of mannuronic oligosaccharides mixture produced by hydrolysis of native polymannuronic is readily calculated from peak area of the species resolved by CE on the basis of a specific degree of polymerization.The influence of the applied electric field strength on the free solution mobility of hyaluronan samples is briefly addressed for molar masses of the order of 10(5) and 10(6) g/mol. The data are compared with the results obtained for a 50 % galactose substituted HA.Mobility data obtained as a function of buffer pH for a native HA sample as well as for two galactose-amide HA derivatives, having slightly different degrees of substitution, are presented and discussed in terms of the polymer charge density parameters ξ.In most cases, more questions than answers arise from the application of CE to charged polysaccharides. However, perspectives are disclosed for a further understanding of the reliability of CE applied for the structural elucidation of such macromolecules.
authors.affiliation: Department of Chemistry and Pharmaceutical Sciences, University of Trieste, Via L. Giorgieri 1, Trieste, 34127, Italy. gamini@units.it.
authors.forename: Amelia
authors.initials: A
authors.lastname: Gamini
authors.affiliation: GSK Vaccines, Manufacturing Science & Technology Bellaria di Rosia, Sovicille (Siena), Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Coslovi
authors.affiliation: GSK Vaccines, Manufacturing Science & Technology Bellaria di Rosia, Sovicille (Siena), Italy.
authors.forename: Mila
authors.initials: M
authors.lastname: Toppazzini
authors.affiliation: Department of Life Sciences, University of Trieste, Via L. Giorgieri 1, Trieste, 34127, Italy.
authors.forename: Isabella
authors.initials: I
authors.lastname: Rustighi
authors.affiliation: GSK Vaccines, Manufacturing Science & Technology Bellaria di Rosia, Sovicille (Siena), Italy.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Campa
authors.affiliation: Broad Institute, Cambridge, MA, USA.
authors.forename: Amedeo
authors.initials: A
authors.lastname: Vetere
authors.affiliation: Department of Life Sciences, University of Trieste, Via L. Giorgieri 1, Trieste, 34127, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Paoletti
mesh_terms: D004040:Dietary Carbohydrates / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004951:Esterification; D006820:Hyaluronic Acid / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004040:Dietary Carbohydrates; D011134:Polysaccharides; D006820:Hyaluronic Acid
keywords: Capillary electrophoresis; Charge density; Charged polysaccharides; Electrophoretic mobility; Glycoconjugates; Hyaluronan; Molar mass distribution; Random degradation