title: Expression of the SOCS family in human chronic wound tissues: Potential implications for SOCS in chronic wound healing.
abstract: Cytokines play important roles in the wound healing process through various signalling pathways. The JAK-STAT pathway is utilised by most cytokines for signal transduction and is regulated by a variety of molecules, including suppressor of cytokine signalling (SOCS) proteins. SOCS are associated with inflammatory diseases and have an impact on cytokines, growth factors and key cell types involved in the wound‑healing process. SOCS, a negative regulator of cytokine signalling, may hold the potential to regulate cytokine‑induced signalling in the chronic wound‑healing process. Wound edge tissues were collected from chronic venous leg ulcer patients and classified as non-healing and healing wounds. The expression pattern of seven SOCSs members, at the transcript and protein level, were examined in these tissues using qPCR and immunohistochemistry. Significantly higher levels of SOCS3 (P=0.0284) and SOCS4 (P=0.0376) in non-healing chronic wounds compared to the healing/healed chronic wounds were observed at the transcript level. Relocalisation of SOCS3 protein in the non-healing wound environment was evident in the investigated chronic biopsies. Thus, the results show that the expression of SOCS transcript indicated that SOCS members may act as a prognostic biomarker of chronic wounds.
authors.affiliation: Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Cardiff University, Cardiff CF14 4XN, UK.
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Cardiff University, Cardiff CF14 4XN, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Sanders
authors.affiliation: Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Cardiff University, Cardiff CF14 4XN, UK.
authors.forename: Fiona
authors.initials: F
authors.lastname: Ruge
authors.affiliation: Wound Healing Research Unit, Cardiff University School of Medicine, Cardiff University, Cardiff CF14 4XN, UK.
authors.forename: Ceri-Ann
authors.initials: CA
authors.lastname: Morris
authors.affiliation: Wound Healing Research Unit, Cardiff University School of Medicine, Cardiff University, Cardiff CF14 4XN, UK.
authors.forename: Keith G
authors.initials: KG
authors.lastname: Harding
authors.affiliation: Cardiff China Medical Research Collaborative, Cardiff University School of Medicine, Cardiff University, Cardiff CF14 4XN, UK.
authors.forename: Wen G
authors.initials: WG
authors.lastname: Jiang
mesh_terms: D002908:Chronic Disease; D015331:Cohort Studies; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D005810:Multigene Family; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D000071223:Suppressor of Cytokine Signaling 3 Protein / Q000235:genetics / Q000378:metabolism; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics* / Q000378:metabolism; D014647:Varicose Ulcer / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing / Q000235:genetics*; D014947:Wounds and Injuries / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D020033:Protein Isoforms; C106644:SOCS3 protein, human; C473084:SOCS4 protein, human; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins