title: Probiotics Supplemented with Omega-3 Fatty Acids are More Effective for Hepatic Steatosis Reduction in an Animal Model of Obesity.
abstract: Today probiotics have been suggested as a treatment for the prevention of NAFLD. Omega-3 fatty acid supplementation may have beneficial effects in regulating hepatic lipid metabolism, adipose tissue function and inflammation. The present study was designed to determine whether probiotics plus omega-3 are superior to probiotics alone on the monosodium glutamate (MSG)-induced NAFLD model in rats. We included 60 rats divided into four groups, 15 animals in each. Rats of group I were intact. Newborn rats of groups II-IV were injected with MSG. The III (Symbiter) group received 2.5 ml/kg of multiprobiotic "Symbiter" containing concentrated biomass of 14 probiotic bacteria genera. The IV (Symbiter-Omega) groups received "Symbiter-Omega" combination of probiotic biomass supplemented with flax and wheat germ oil (250 mg of each, concentration of omega-3 fatty acids 1-5 %). In both interventional groups reduction in total NAS score was observed. Supplementation of alive probiotic mixture with omega-3 fatty acids lead to 20 % higher decrease in steatosis score (0.73 ± 0.11 vs 0.93 ± 0.22, p = 0.848) and reduction by 16.6 % of triglycerides content in liver as compared to probiotic alone. Our study demonstrated more pronounced reduction in hepatic steatosis and hepatic lipid accumulation after treatment with combination of alive probiotics and omega-3 as compared to probiotics alone.
authors.affiliation: Bogomolets National Medical University, T. Shevchenko Boulevard, 13, Kiev, 01601, Ukraine. nazariikobyliak@gmail.com.
authors.forename: Nazarii
authors.identifier: 0000-0001-9814-689X
authors.initials: N
authors.lastname: Kobyliak
authors.affiliation: Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64/13, Kiev, 01601, Ukraine.
authors.forename: Tetyana
authors.initials: T
authors.lastname: Falalyeyeva
authors.affiliation: Bogomolets National Medical University, T. Shevchenko Boulevard, 13, Kiev, 01601, Ukraine.
authors.forename: Petro
authors.initials: P
authors.lastname: Bodnar
authors.affiliation: Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64/13, Kiev, 01601, Ukraine.
authors.forename: Tetyana
authors.initials: T
authors.lastname: Beregova
mesh_terms: D000818:Animals; D019587:Dietary Supplements / Q000032:analysis; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism; D019936:Probiotics / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D012970:Sodium Glutamate / Q000009:adverse effects; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D014280:Triglycerides; D012970:Sodium Glutamate
keywords: Hepatic steatosis; Non-alcoholic fatty liver disease; Omega-3 fatty acids; Probiotics