title: Characteristics Analysis of F1 Hybrids between Genetically Modified Brassica napus and B. rapa.
abstract: A number of studies have been conducted on hybridization between transgenic Brassica napus and B. rapa or backcross of F1 hybrid to their parents. However, trait changes must be analyzed to evaluate hybrid sustainability in nature. In the present study, B. rapa and transgenic (BrAGL20) B. napus were hybridized to verify the early flowering phenomenon of F1 hybrids, and F1 hybrid traits were analyzed to predict their impact on sustainability. Flowering of F1 hybrid has been induced slightly later than that of the transgenic B. napus, but flowering was available in the greenhouse without low temperature treatment to young plant, similar to the transgenic B. napus. It is because the BrAGL20 gene has been transferred from transgenic B. napus to F1 hybrid. The size of F1 hybrid seeds was intermediate between those of B. rapa and transgenic B. napus, and ~40% of F1 pollen exhibited abnormal size and morphology. The form of the F1 stomata was also intermediate between that of B. rapa and transgenic B. napus, and the number of stomata was close to the parental mean. Among various fatty acids, the content of erucic acid exhibited the greatest change, owing to the polymorphism of parental FATTY ACID ELONGASE 1 alleles. Furthermore, F2 hybrids could not be obtained. However, BC1 progeny were obtained by hand pollination of B. rapa with F1 hybrid pollen, with an outcrossing rate of 50%.
authors.affiliation: Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, 370 Nongsaengmyeong-ro, Wansan-gu, Jeonju, North Jeolla Province, 54874, Republic of Korea.
authors.forename: Soo-In
authors.initials: SI
authors.lastname: Sohn
authors.affiliation: Institute for Future Environmental Ecology Co., Ltd, 5, Palbok 1-gil, Deokjin-gu, Jeonju, North Jeolla Province, 54883, Republic of Korea.
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Oh
authors.affiliation: Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, 370 Nongsaengmyeong-ro, Wansan-gu, Jeonju, North Jeolla Province, 54874, Republic of Korea.
authors.forename: Kyeong-Ryeol
authors.initials: KR
authors.lastname: Lee
authors.affiliation: National Agrobiodiversity Center, National Institute of Agricultural Sciences, 370 Nongsaengmyeong-ro, Wansan-gu, Jeonju, North Jeolla Province, 54874, Republic of Korea.
authors.forename: Ho-Cheol
authors.initials: HC
authors.lastname: Ko
authors.affiliation: Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, 370 Nongsaengmyeong-ro, Wansan-gu, Jeonju, North Jeolla Province, 54874, Republic of Korea.
authors.forename: Hyun-Suk
authors.initials: HS
authors.lastname: Cho
authors.affiliation: Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, 370 Nongsaengmyeong-ro, Wansan-gu, Jeonju, North Jeolla Province, 54874, Republic of Korea.
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, 370 Nongsaengmyeong-ro, Wansan-gu, Jeonju, North Jeolla Province, 54874, Republic of Korea.
authors.forename: Ancheol
authors.initials: A
authors.lastname: Chang
mesh_terms: D029688:Brassica napus / Q000235:genetics*; D029741:Brassica rapa / Q000235:genetics*; D005434:Flow Cytometry; D017343:Genes, Plant; D006824:Hybridization, Genetic; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction