title: Chromatin Preparation and Chromatin Immuno-precipitation from Drosophila Embryos.
abstract: This protocol provides specific details on how to perform Chromatin immunoprecipitation (ChIP) from Drosophila embryos. ChIP allows the matching of proteins or histone modifications to specific genomic regions. Formaldehyde-cross-linked chromatin is isolated and antibodies against the target of interest are used to determine whether the target is associated with a specific DNA sequence. This can be performed in spatial and temporal manner and it can provide information about the genome-wide localization of a given protein or histone modification if coupled with deep sequencing technology (ChIP-Seq).
authors.affiliation: Max Planck Institute of Immunobiology and Epigenetics, Stübeweg 51, Freiburg, 79108, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Löser
authors.affiliation: Max Planck Institute of Immunobiology and Epigenetics, Stübeweg 51, Freiburg, 79108, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Latreille
authors.affiliation: Max Planck Institute of Immunobiology and Epigenetics, Stübeweg 51, Freiburg, 79108, Germany.
authors.forename: Nicola
authors.initials: N
authors.lastname: Iovino
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000302:isolation & purification*; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D004330:Drosophila / Q000196:embryology / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D042421:Histone Code / Q000235:genetics; D006657:Histones / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D002843:Chromatin; D006657:Histones
keywords: ChIP-Seq; Chromatin immunoprecipitation; Drosophila embryos