title: Fibrin-alginate hydrogel supports steroidogenesis, in vitro maturation of oocytes and parthenotes production from caprine preantral follicles cultured in group.
abstract: This study aimed to establish a culture system that improves the in vitro development of caprine preantral follicles. In a first experiment, follicles were encapsulated as a single unit per bead and cultured singly or in groups or with five follicles in the same alginate (ALG) bead for 18 days. In a subsequent experiment, the "five follicles per bead" design was chosen to culture in ALG, fibrin-alginate (FA) or hyaluronate (HA) for 18 days. In a third experiment, we chose the five follicles per bead in FA to culture for 30 days. The culture set-up of five follicles per ALG bead increased antrum formation and follicle diameter compared to the other culture designs (p < .05). Moreover, under this condition, 44.44% of the oocytes from in vitro cultured preantral follicles reached meiotic resumption. A significant increase of follicle diameter occurred in attachment system and FA (p < .05), but the ALG condition reached the highest among all groups on day 18 (p < .05). Follicles encapsulated in matrix produced more estradiol and progesterone than attachment system (p < .05). The expression of MMP-9 mRNA was higher in FA than in other groups (p < .05) and similar to antral follicles from in vivo control (p > .05). Only FA group resulted in oocytes matured. After 30 days, oocytes from preantral follicles in vitro grown in FA developed to eight-cell parthenotes. In conclusion, a culture system using FA supported the development of caprine preantral follicles cultured in group and included in the same bead of hydrogel, improving the oocyte maturation and producing parthenotes.
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: I R
authors.initials: IR
authors.lastname: Brito
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: G M
authors.initials: GM
authors.lastname: Silva
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: A D
authors.initials: AD
authors.lastname: Sales
authors.affiliation: Department of Animal Science, Laboratory of Animal Physiology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: C H
authors.initials: CH
authors.lastname: Lobo
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Rodrigues
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: R F
authors.initials: RF
authors.lastname: Sousa
authors.affiliation: Department of Animal Science, Laboratory of Animal Physiology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Aaa
authors.initials: A
authors.lastname: Moura
authors.affiliation: Biotechnology Laboratory, University of Fortaleza, Fortaleza, CE, Brazil.
authors.forename: Cem
authors.initials: C
authors.lastname: Calderón
authors.affiliation: Biotechnology Laboratory, University of Fortaleza, Fortaleza, CE, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Bertolini
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.affiliation: Follicle Biology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Smitz
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D005260:Female; D005337:Fibrin / Q000737:chemistry / Q000494:pharmacology*; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006041:Goats / Q000502:physiology*; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary*; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism; D006080:Ovarian Follicle / Q000502:physiology*; D010312:Parthenogenesis
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid; D005337:Fibrin