title: Analysis of Endogenous Protein Interactions of Polycomb Group of Proteins in Mouse Embryonic Stem Cells.
abstract: PRC1 complexes contain four core subunits: Pcgf, Phc, Ring1, and Cbx proteins. Interestingly, mammalian genomes have several paralogues for each subunit, which are differentially expressed depending on the cell type, differentiation program, and cellular stimuli. Therefore, identification and characterization of the specific architecture of different PRC1 complexes during cellular differentiation are essential to better understand the function and recruitment mechanism of PRC1 complexes. In this chapter we describe several methods to study Polycomb architecture, and identification of novel interactors in both pluripotent and differentiating mouse embryonic stem cells.
authors.affiliation: Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003, Barcelona, Spain. lmorey@med.miami.edu.|Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, 1475 N.W. 12th Avenue, Miami, FL, 33136, USA. lmorey@med.miami.edu.
authors.forename: Lluis
authors.initials: L
authors.lastname: Morey
authors.affiliation: Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003, Barcelona, Spain.|Institució Catalana de Recerca i Estudis Avançats, Pg. Lluis Companys 23, 08010, Barcelona, Spain.
authors.forename: Luciano
authors.initials: L
authors.lastname: Di Croce
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D008967:Molecular Biology / Q000379:methods*; D000066450:Mouse Embryonic Stem Cells / Q000378:metabolism; D063150:Polycomb Repressive Complex 1 / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D063146:Polycomb-Group Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D006657:Histones; D063146:Polycomb-Group Proteins; D063150:Polycomb Repressive Complex 1
keywords: Differentiation; Immunoprecipitation; Pluripotent stem cells; Polycomb