title: miR-186 inhibits cell proliferation of prostate cancer by targeting GOLPH3.
abstract: Prostate cancer is one of the leading causes of cancer deaths among men, many miRNAs have been demonstrated to play critical role in the progression of prostate cancer, miR-186 suppresses the progression of many tumors, such as bladder cancer and glioma. Previous study shows miR-186 is downregulated in prostate cancer tissues, and is a good prognosis for prostate cancer patients. In this study, we found miR-186 was downregulated in prostate cancer cells and tissues, overexpression of miR-186 inhibited cell proliferation and tumorigenesis in vitro determined by MTT assay, colony formation assay and soft agar growth assay, whereas knockdown of miR-186 reduced these effects. Cell cycle analysis found miR-186 overexpression arrested cell cycle in G0/G1 phase, and reduced p21 and p27 levels, and enhanced Cyclin D1 and the phosphorylation of Rb levels, suggesting miR-186 blocked G1/S transition. A novel oncogene Golgi phhosphoprotein 3 (GOLPH3) was the target of miR-186, miR-186 bound to the 3'UTR of GOLPH3. Moreover, miR-186 was negatively correlated with GOLPH3 in prostate cancer tissues. In conclusion, our study suggested miR-186 inhibited cell proliferation through targeting oncogene GOLPH3.
authors.affiliation: Department of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University Guangzhou, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Hua
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University Guangzhou, Guangdong, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Urology, Guangzhou Red Cross Hospital, Medical College, Jinan University Guangzhou, China.
authors.forename: Wenhai
authors.initials: W
authors.lastname: Pan
authors.affiliation: Department of Physical Examination Centre, Guangzhou Red Cross Hospital, Medical College, Jinan University Guangzhou, China.
authors.forename: Meiyun
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University Guangzhou, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University Guangzhou, China.
authors.forename: Zexiao
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Department of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University Guangzhou, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xian
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical UniversityGuangzhou, Guangdong, China; Department of Pathology, School of Basic Medical Sciences, Southern Medical UniversityGuangzhou, Guangdong, China; Guangdong Provincial Key Laboratory of Molecular Tumor PathologyGuangzhou, Guangdong, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Yu
keywords: GOLPH3; cell proliferation; miR-186; prostate cancer