title: Enhancement of docosahexaenoic acid production by low-energy ion implantation coupled with screening method based on Sudan black B staining in Schizochytrium sp.
abstract: Schizochytrium sp. is a hopeful docosahexaenoic acid (DHA) producing candidate due to its rapid growth rate and high DHA proportion in total lipid content. In this study, low-energy ion implantation was applied to Schizochytrium sp. to induce high DHA-producing mutants. Screening these mutants by Sudan black B staining, a mutant strain S1 which showed a 61% improvement in DHA production than that of the parent strain was successfully selected. Subsequently, parameters of DHA production of mutant strain S1 were optimized in a 500-mL Erlenmeyer flask. Under the optimum fermentation conditions, the production of DHA and the percentage of DHA in total lipid of mutant strain S1 were 6.52g/L and 46.2%, respectively. This study provides an effective breeding strategy for improved DHA production of Schizochytrium sp. through combination of the novel mutagenesis technology, the effective screening method and fermentation optimization.
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Wuhan, Hubei 430023, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Fu
authors.affiliation: Wuhan Institute of Virology, Chinese Academy of Sciences, 44 Xiaohongshan, Wuhan, Hubei 430071, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Wuhan, Hubei 430023, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Lu
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Wuhan, Hubei 430023, PR China.
authors.forename: Yuanfeng
authors.initials: Y
authors.lastname: Lin
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Wuhan, Hubei 430023, PR China.
authors.forename: Xinlei
authors.initials: X
authors.lastname: Xie
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Wuhan, Hubei 430023, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Wuhan, Hubei 430023, PR China. Electronic address: zhengc1314@163.com.
authors.forename: Cao
authors.initials: C
authors.lastname: Zheng
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Wuhan, Hubei 430023, PR China.
authors.forename: Dongping
authors.initials: D
authors.lastname: He
mesh_terms: D001391:Azo Compounds; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D005285:Fermentation; D016296:Mutagenesis; D009281:Naphthalenes; D013194:Staining and Labeling; D058009:Stramenopiles / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D001391:Azo Compounds; D009281:Naphthalenes; D004281:Docosahexaenoic Acids; C016118:Sudan Black B
keywords: DHA; Lipid; Low-energy ion implantation; Schizochytrium sp.; Sudan black B staining