title: Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9.
abstract: Balancer chromosomes are convenient tools used to maintain lethal mutations in heterozygotes. We established a method for engineering new balancers in C. elegans by using the CRISPR/Cas9 system in a non-homologous end-joining mutant. Our studies will make it easier for researchers to maintain lethal mutations and should provide a path for the development of a system that generates rearrangements at specific sites of interest to model and analyse the mechanisms of action of genes.
authors.affiliation: Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Iwata
authors.affiliation: Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo, Japan.
authors.forename: Sawako
authors.initials: S
authors.lastname: Yoshina
authors.affiliation: Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Suehiro
authors.affiliation: Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Hori
authors.affiliation: Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo, Japan.|Tokyo Women's Medical University Institute for Integrated Medical Sciences, Tokyo, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Mitani
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D064113:CRISPR-Cas Systems; D017173:Caenorhabditis elegans / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D009154:Mutation