title: Photokinesis and Djopsin gene expression analysis during the regeneration of planarian eyes.
abstract: Planarians provide the ideal model for studying eye development, with their simple eye structure and exceptionally rapid regeneration. Here, we observed the eye morphogenesis, photophobic behavior, spectral sensitivity and expression pattern of Djopsin in the freshwater planarian Dugesia japonica. The results showed that: (i) Djopsin encoding the putative protein belonged to the rhabdomeric opsins group and displayed high conservation during animal evolution; (ii) planarians displayed diverse photophobic response to different visible wavelengths and were more sensitive to light blue (495 nm) and yellow (635 nm); (iii) the morphogenesis and functional recovery of eyes were related to the expression pattern of Djopsin during head regeneration; and (iv) Djopsin gene plays a major role in functional recovery during eye regeneration and visual system maintenance in adult planarians.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Zimei
authors.initials: Z
authors.lastname: Dong
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Yuwen
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Yingxu
authors.initials: Y
authors.lastname: Sima
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Dong
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Huina
authors.initials: H
authors.lastname: Zhan
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Guangwen
authors.initials: G
authors.lastname: Chen
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Dezeng
authors.initials: D
authors.lastname: Liu
mesh_terms: D000818:Animals; D005123:Eye / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D008027:Light; D009024:Morphogenesis; D009799:Ocular Physiological Phenomena; D055355:Opsins / Q000235:genetics*; D000071444:Phototaxis; D010932:Planarians / Q000235:genetics* / Q000254:growth & development*; D034622:RNA Interference; D012038:Regeneration / Q000235:genetics*
chemical_list: D055355:Opsins
keywords: eye regeneration; opsin; photophobic response; planarian