title: Flavonoid compounds from the red marine alga Alsidium corallinum protect against potassium bromate-induced nephrotoxicity in adult mice.
abstract: Potassium bromate (KBrO3), an environmental pollutant, is a well-known human carcinogen and a potent nephrotoxic agent. Currently, natural products have built a well-recognized role in the management of many diseases induced by pollutants. As potent natural sources of bioactive compounds, marine algae have been demonstrated to be rich in novel secondary metabolites with a broad range of biological functions. In this study, adults male mice were orally treated for 15 days with KBrO3(0.5 g/L) associated or not with extract of Alsidium corallinum, a red Mediterranean alga. In vitro study demonstrated that algal extract has antioxidant efficacy attributable to the presence of flavonoids and polyphenols. Among these, Liquid chromatography-mass spectrometry analysis showed A. corallinum is rich in kaempferol, apigenin, catechin, and quercetin flavonoids. In vivo study showed that supplementation with the alga significantly prevented KBrO3-induced nephrotoxicity as indicated by plasma biomarkers (urea, uric acid, and creatinin levels) and oxidative stress related parameters (malondialdehyde, superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione, vitamin C, hydrogen peroxide, protein oxidation products) in kidney tissue. The corrective effect of A. corallinum on KBrO3-induced kidney injury was also supported by molecular and histopathological observations. In conclusion, it was established that the red alga, thanks to its bioactive compounds, effectively counteracts toxic effects of KBrO3and could be a useful coadjuvant agent for treatment of this pollutant poisonings. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1475-1486, 2017.
authors.affiliation: Laboratoire de Pharmacologie, Faculté de Médecine de Sfax, UR/12 ES-13, Université de Sfax, Sfax, 3029, Tunisie.
authors.forename: Hajer
authors.initials: H
authors.lastname: Ben Saad
authors.affiliation: EA 2219 Géoarchitecture, Université Bretagne Occidentale, UFR Sciences et Techniques, 6 Avenue V. Le Gorgeu, CS 93 837, Brest Cedex 3, 29238, France.
authors.forename: Manel
authors.initials: M
authors.lastname: Gargouri
authors.affiliation: Laboratoire d'Enzymes et Bioconversions, Ecole d'Ingénieurs de Sfax, Université de Sfax, BP 1173, Sfax, 3038, Tunisie.
authors.forename: Fatma
authors.initials: F
authors.lastname: Kallel
authors.affiliation: Laboratoire de Biochimie, CHU Hedi Chaker, Université de Sfax, Sfax, 3029, Tunisie.
authors.forename: Rim
authors.initials: R
authors.lastname: Chaabene
authors.affiliation: Laboratoire d'Anatomopathologie, CHU Habib Bourguiba, Université de Sfax, Sfax, 3029, Tunisie.
authors.forename: Tahia
authors.initials: T
authors.lastname: Boudawara
authors.affiliation: Laboratoire de Biochimie, CHU Hedi Chaker, Université de Sfax, Sfax, 3029, Tunisie.
authors.forename: Kamel
authors.initials: K
authors.lastname: Jamoussi
authors.affiliation: EA 2219 Géoarchitecture, Université Bretagne Occidentale, UFR Sciences et Techniques, 6 Avenue V. Le Gorgeu, CS 93 837, Brest Cedex 3, 29238, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Magné
authors.affiliation: Laboratoire de Pharmacologie, Faculté de Médecine de Sfax, UR/12 ES-13, Université de Sfax, Sfax, 3029, Tunisie.
authors.forename: Khaled
authors.initials: K
authors.lastname: Mounir Zeghal
authors.affiliation: Laboratoire de Pharmacologie, Faculté de Médecine de Sfax, UR/12 ES-13, Université de Sfax, Sfax, 3029, Tunisie.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Hakim
authors.affiliation: Institut Supérieur de Biotechnologie de Sfax, Université de Sfax, Sfax, 3000, Tunisie.
authors.forename: Ibtissem
authors.initials: I
authors.lastname: Ben Amara
mesh_terms: D000367:Age Factors; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D001959:Bromates / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D019610:Cytoprotection / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D000461:Rhodophyta / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001959:Bromates; D002273:Carcinogens; D005419:Flavonoids; D020011:Protective Agents; C019536:potassium bromate
keywords: Alsidium corallinum; flavonoids; kidney; mice; oxidative stress; potassium bromate intoxication