title: Specific histamine binding activity of a new lipocalin from Hyalomma asiaticum (Ixodidae) and therapeutic effects on allergic asthma in mice.
abstract: Lipocalin proteins are secreted by tick salivary glands as an important strategy to interfere with the immune response of hosts. A large number of lipocalins are secreted, but the functions of most of these proteins are unclear. Here, we report a new lipocalin protein with particular histamine binding capacity, which was isolated from the salivary glands of the tick Hyalomma asiaticum.
  
  
  
  The full length cDNA of the Ha24 gene was obtained by RACE, and Ha24 gene was expressed in E. coli; after protein purification and mice immunizations, specific Polyclonal antibodies (PcAb) were created in response to the recombinant protein. Reverse transcription PCR (RT-PCR), Quantitative PCR (Q-PCR), indirect immunofluorescence antibody (IFA) assay and western blot were used to detect the existence of native Ha24 in ticks. To confirm the histamine-binding capacity of rHa24, a histamine-binding assay was completed in vitro (ELISA) and in vivo by inhibition of allergic asthma in mice.
  
  
  
  Ha24 is coded by 681 bases, contains 227 amino acids, and has a molecular weight of 23.3 kDa. Abundant expression in the salivary glands of feeding ticks was confirmed by the identification of native Ha24 in ticks. The results of a histamine binding assay both in vitro and in vivo demonstrated that rHa24 binds specifically with histamine in a dose-dependent manner, and can provide relief from allergic asthma in mice.
  
  
  
  Ha24 is a new tick lipocalin with specific histamine binding activity that can provide relief from host inflammation response.
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Chizhou Vocational and Technique Colleague, Chizhou, 247000, China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
authors.forename: Yongzhi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Cao
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
authors.forename: Houshuang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Gong
authors.affiliation: Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China. jinlinzhou@shvri.ac.cn.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. jinlinzhou@shvri.ac.cn.
authors.forename: Jinlin
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001249:Asthma / Q000188:drug therapy; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006632:Histamine / Q000378:metabolism*; D019476:Insect Proteins / Q000302:isolation & purification / Q000378:metabolism*; D026863:Ixodidae / Q000737:chemistry*; D054834:Lipocalins / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding; D012469:Salivary Glands / Q000737:chemistry*; D045506:Therapeutic Uses
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D054834:Lipocalins; D045506:Therapeutic Uses; D006632:Histamine
keywords: Allergic asthma in mice; Histamine binding; Hyalomma asiaticum; Lipocalin