title: Distinctive expression of interleukin-23 receptor subunits on human Th17 and γδ T cells.
abstract: The interleukin-23 (IL-23) pathway, T helper 17 (Th17) cells and γδ T cells, which respond to IL-23, have major pro-inflammatory roles. We have used unique IL-23 receptor (IL-23R) subunit-specific monoclonal antibodies, X67 and X68, and IL-12 receptor beta-1 subunit (IL-12Rβ1) expression levels to evaluate the IL-23R complex on CD4 αβ TCR Th17 cells and on γδ T cells. Both IL-23R and IL-12Rβ1 subunits constitute the functional IL-23R. Expression of the IL-23R subunit by cultured Th17 cells was heterogeneous. Th17 cells expressed consistent high levels of the IL-12Rβ1 subunit, which appeared a better predictor of responsiveness to IL-23 than the expression of the IL-23R subunit. Moreover, sorting memory CD4 T cells by high IL-12Rβ1 expression selectively enriched cells committed to IL-17 production from the blood. IL-23R expression was also observed on freshly isolated and cultured γδ T cells and the cultured γδ T cells were not responsive to IL-23.
authors.affiliation: Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria, Australia.|Department of Immunology, Monash University Central Clinical School, Melbourne, Victoria, Australia.|Department of Pathology, The University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Wines
authors.affiliation: Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria, Australia.
authors.forename: May L
authors.initials: ML
authors.lastname: Yap
authors.affiliation: Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria, Australia.|Department of Pathology, The University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Maree S
authors.initials: MS
authors.lastname: Powell
authors.affiliation: Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria, Australia.|Department of Pathology, The University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Peck-Szee
authors.initials: PS
authors.lastname: Tan
authors.affiliation: Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria, Australia.
authors.forename: K Kerry
authors.initials: KK
authors.lastname: Ko
authors.affiliation: Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria, Australia.
authors.forename: Eva
authors.initials: E
authors.lastname: Orlowski
authors.affiliation: Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria, Australia.|Department of Immunology, Monash University Central Clinical School, Melbourne, Victoria, Australia.|Department of Pathology, The University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: P Mark
authors.initials: PM
authors.lastname: Hogarth
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D007156:Immunologic Memory; D053711:Interleukin-12 Receptor beta 1 Subunit / Q000378:metabolism*; D051379:Mice; D021122:Protein Subunits / Q000378:metabolism*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000378:metabolism*; D058504:Th17 Cells / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; C515500:IL23R protein, human; D053711:Interleukin-12 Receptor beta 1 Subunit; D021122:Protein Subunits; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018123:Receptors, Interleukin