title: Efficacy of the Olyset Duo net against insecticide-resistant mosquito vectors of malaria.
abstract: Olyset Duo is a new long-lasting insecticidal net treated with permethrin (a pyrethroid) and pyriproxyfen, an insect growth regulator that disrupts the maturation of oocytes in mosquitoes exposed to the net. We tested the Olyset Duo net against pyrethroid-resistant Anopheles gambiae mosquitoes, which transmit malaria parasites, in laboratory bioassays and in a trial in Benin using experimental huts that closely resemble local habitations. Host-seeking mosquitoes that entered to feed were free to contact the occupied nets and were collected the next morning from exit traps. Surviving blood-fed mosquitoes were observed for effects on reproduction. Control nets were treated with pyrethroid only or pyriproxyfen only, and nets were tested unwashed and after 20 standardized washes. The Olyset Duo net showed improved efficacy and wash resistance relative to the pyrethroid-treated net in terms of mosquito mortality and prevention of blood feeding. The production of offspring among surviving blood-fed A. gambiae in the hut trial was reduced by the pyriproxyfen-treated net and the Olyset Duo net both before washing (90 and 71% reduction, respectively) and after washing (38 and 43% reduction, respectively). The degree of reproductive suppression in the hut trial was predicted by laboratory tunnel tests but not by cone bioassays. The overall reduction in reproductive rate of A. gambiae with the Olyset Duo net in the trial was 94% with no washing and 78% after 20 washes. The Olyset Duo net has the potential to provide community control of mosquito populations and reduce malaria transmission in areas of high insecticide resistance.
authors.affiliation: London School of Hygiene and Tropical Medicine, London WC1E 7HT, U.K. Centre de Recherches Entomologiques de Cotonou, Ministry of Health, Cotonou 06 BP 2604, Benin. Pan African Malaria Vector Research Consortium, London WC1E 7HT, U.K. corine.ngufor@lshtm.ac.uk.
authors.forename: Corine
authors.initials: C
authors.lastname: Ngufor
authors.affiliation: London School of Hygiene and Tropical Medicine, London WC1E 7HT, U.K. Centre de Recherches Entomologiques de Cotonou, Ministry of Health, Cotonou 06 BP 2604, Benin. Pan African Malaria Vector Research Consortium, London WC1E 7HT, U.K.
authors.forename: Raphael
authors.initials: R
authors.lastname: N'Guessan
authors.affiliation: Centre de Recherches Entomologiques de Cotonou, Ministry of Health, Cotonou 06 BP 2604, Benin.
authors.forename: Josias
authors.initials: J
authors.lastname: Fagbohoun
authors.affiliation: Centre de Recherches Entomologiques de Cotonou, Ministry of Health, Cotonou 06 BP 2604, Benin.
authors.forename: Damien
authors.initials: D
authors.lastname: Todjinou
authors.affiliation: Centre de Recherches Entomologiques de Cotonou, Ministry of Health, Cotonou 06 BP 2604, Benin.
authors.forename: Abibath
authors.initials: A
authors.lastname: Odjo
authors.affiliation: Innovative Vector Control Consortium, Liverpool L3 5QA, U.K.
authors.forename: David
authors.initials: D
authors.lastname: Malone
authors.affiliation: Liverpool School of Tropical Medicine, Liverpool L3 5QA, U.K.
authors.forename: Hanafy
authors.initials: H
authors.lastname: Ismail
authors.affiliation: Centre de Recherches Entomologiques de Cotonou, Ministry of Health, Cotonou 06 BP 2604, Benin.
authors.forename: Martin
authors.initials: M
authors.lastname: Akogbeto
authors.affiliation: London School of Hygiene and Tropical Medicine, London WC1E 7HT, U.K. Pan African Malaria Vector Research Consortium, London WC1E 7HT, U.K.
authors.forename: Mark
authors.initials: M
authors.lastname: Rowland
mesh_terms: D000818:Animals; D000852:Anopheles; D001681:Biological Assay; D005260:Female; D006801:Humans; D007305:Insecticide Resistance; D057148:Insecticide-Treated Bednets; D007306:Insecticides; D008288:Malaria / Q000517:prevention & control* / Q000635:transmission; D000072138:Mosquito Vectors; D026023:Permethrin; D011722:Pyrethrins; D011725:Pyridines; D057170:Translational Research, Biomedical; D014944:World Health Organization
chemical_list: D007306:Insecticides; D011722:Pyrethrins; D011725:Pyridines; C055613:pyriproxyfen; D026023:Permethrin