title: Identification of haemoglobin New York by haemoglobin A1cmeasurement using the Sebia Capillarys 2 Flex Piercing system.
abstract: Haemoglobinopathies may interfere with the haemoglobin A1c(HbA1c) measurement, leading to incorrect diagnosis and inappropriate treatment. It is essential that HbA1cassays are capable of identifying haemoglobinopathies. We report two cases of haemoglobin New York (HbNY) discovered through HbA1canalysis using capillary electrophoresis (Capillarys 2 Flex Piercing [C2FP], Sebia). We used these samples to evaluate the ability of three other HbA1cassays to identify this variant: ion-exchange high-performance liquid chromatography (Variant II Turbo [VII-T], Bio-Rad); boronate affinity high-performance liquid chromatography (Ultra2, Trinity Biotech) and immunoassay (Cobas c501 Tina-quant Generation 3, Roche Diagnostics). Each method was used for HbA1cassay of in samples from two cases of heterozygous haemoglobinopathy: β0-thalassemia/HbNY (Case 1) and HbA/NY (Case 2). Only the C2FP system detected HbNY (an additional peak appeared between HbA1cand HbA0). Clinical laboratories should be aware of the limitations of their HbA1cassay methods especially in geographic areas, where haemoglobinopathy prevalence is high.
authors.affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chao
authors.affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Zemin
authors.initials: Z
authors.lastname: Wan
authors.affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Xinzhong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Yunxiu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Peifeng
authors.initials: P
authors.lastname: Ke
authors.affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Zhuang
authors.affiliation: Department of Laboratory Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Xianzhang
authors.initials: X
authors.lastname: Huang
mesh_terms: D000368:Aged; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D006442:Glycated Hemoglobin / Q000302:isolation & purification*; D006455:Hemoglobins, Abnormal / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D055815:Young Adult; D017086:beta-Thalassemia / Q000097:blood / Q000175:diagnosis*
chemical_list: D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal; C517652:hemoglobin A1c protein, human; C032181:hemoglobin New York
keywords: Haemoglobin A1c; boronate affinity high-performance liquid chromatography; capillary electrophoresis; haemoglobin New York; immunoassay; ion-exchange high-performance liquid chromatography