title: Porcine epidemic diarrhea virus nucleoprotein contributes to HMGB1 transcription and release by interacting with C/EBP-β.
abstract: Porcine epidemic diarrhea is a devastating swine enteric disease, which is caused by porcine epidemic diarrhea virus (PEDV) infection. Our studies demonstrated that PEDV infection resulted in the up-regulation of proinflammatory cytokines. Meanwhile, PEDV infection and overexpression of viral nucleoprotein resulted in the acetylation and release of high mobility group box 1 proteins in vitro, an important proinflammatory response mediator, which contributes to the pathogenesis of various inflammatory diseases. Our studies also showed that SIRT1, histone acetyltransferase, and NF-κB regulated the acetylation and release of HMGB1. Chromatin immunoprecipitation, dual-luciferase reporter gene assay, and co-immunoprecipitation experiments illustrated that PEDV-N could induce HMGB1 transcription by interacting with C/EBP-β, which could bind to C/EBP motif in HMGB1 promotor region. Collectively, our data indicate PEDV-N contributes to HMGB1 transcription and the subsequent release/acetylation of HMGB1 during PEDV infection.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China, 210095.
authors.forename: Chang-Chao
authors.initials: CC
authors.lastname: Huan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China, 210095.
authors.forename: Hua-Xia
authors.initials: HX
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China, 210095.
authors.forename: Xiang-Xiang
authors.initials: XX
authors.lastname: Sheng
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China, 210095.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China, 210095.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, 200241.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, 200241.
authors.forename: Qin-Fang
authors.initials: QF
authors.lastname: Liu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, 200241.
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Tong
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, 200241.
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China, 210095.
authors.forename: Hong-Jie
authors.initials: HJ
authors.lastname: Fan
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong Province, China, 250100.
authors.forename: Jia-Qiang
authors.initials: JQ
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China, 210095.|Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China, 200241.
authors.forename: Xiang
authors.initials: X
authors.lastname: Mao
mesh_terms: D000107:Acetylation; D000818:Animals; D001665:Binding Sites; D015415:Biomarkers; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism*; D002522:Chlorocebus aethiops; D018352:Coronavirus Infections / Q000235:genetics / Q000378:metabolism / Q000821:virology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D024243:HMGB1 Protein / Q000235:genetics* / Q000378:metabolism; D051548:Histone Acetyltransferases / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism*; D059372:Nucleotide Motifs; D053485:Porcine epidemic diarrhea virus / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D056564:Sirtuin 1 / Q000378:metabolism; D014158:Transcription, Genetic; D014709:Vero Cells
chemical_list: D015415:Biomarkers; D022782:CCAAT-Enhancer-Binding Protein-beta; D016207:Cytokines; D024243:HMGB1 Protein; D018836:Inflammation Mediators; D016328:NF-kappa B; D009698:Nucleoproteins; D051548:Histone Acetyltransferases; D056564:Sirtuin 1
keywords: C/EBP-β; HMGB1; nucleoprotein; porcine epidemic diarrhea virus