title: Genetic characterization of two fully sequenced multi-drug resistant plasmids pP10164-2 and pP10164-3 from Leclercia adecarboxylata.
abstract: We previously reported the complete sequence of the resistance plasmid pP10164-NDM, harboring blaNDM(conferring carbapenem resistance) and bleMBL(conferring bleomycin resistance), which is recovered from a clinical Leclercia adecarboxylata isolate P10164 from China. This follow-up work disclosed that there were still two multidrug-resistant (MDR) plasmids pP10164-2 and pP10164-3 coexisting in this strain. pP10164-2 and pP10164-3 were completely sequenced and shown to carry a wealth of resistance genes, which encoded the resistance to at least 10 classes of antibiotics (β-lactams. macrolides, quinolones, aminoglycosides, tetracyclines, amphenicols, quaternary ammonium compounds, sulphonamides, trimethoprim, and rifampicin) and 7 kinds of heavy mental (mercury, silver, copper, nickel, chromate, arsenic, and tellurium). All of these antibiotic resistance genes are associated with mobile elements such as transposons, integrons, and insertion sequence-based transposable units, constituting a total of three novel MDR regions, two in pP10164-2 and the other one in pP10164-3. Coexistence of three resistance plasmids pP10164-NDM, pP10164-2 and pP10164-3 makes L. adecarboxylata P10164 tend to become extensively drug-resistant.
authors.affiliation: Department of Pharmacy, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Fengjun
authors.initials: F
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.|State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Yigang
authors.initials: Y
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.|College of Food Science and Project Engineering, Bohai University, Jinzhou 121013, China.
authors.forename: Defu
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Chen
authors.affiliation: Transfusion Department, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Yahan
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Pharmacy, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Xia