title: A rapid method for the simultaneous determination of 25 anti-hypertensive compounds in dietary supplements using ultra-high-pressure liquid chromatography.
abstract: A novel, stable, simple and specific ultra-performance liquid chromatography method with ultraviolet detection (205 nm) for the simultaneous analysis of 25 anti-hypertensive substances was developed. The method was validated according to the International Conference of Harmonisation guidelines with respect to linearity, accuracy, precision, limit of detection (LOD), limit of quantitation (LOQ) and stability. From the ultra-performance liquid chromatography results, we identified the LOD and LOQ of solid samples to be 0.20-1.00 and 0.60-3.00 μg ml-1, respectively, while those of liquid samples were 0.30-1.20 and 0.90-3.60 μg ml-1, respectively. The linearity exceeded 0.9999, and the intra- and inter-day precisions were 0.15-6.48% and 0.28-8.67%, respectively. The intra- and inter-day accuracies were 82.25-111.42% and 80.70-115.64%, respectively, and the stability was lower than 12.9% (relative standard deviation). This method was applied to the monitoring of 97 commercially available dietary supplements obtained in Korea, such as pills, soft capsules, hard capsules, liquids, powders and tablets. The proposed method is accurate, precise and of high quality, and can be used for the routine, reproducible analysis and control of 25 anti-hypertensive substances in various dietary supplements. The work presented herein may help to prevent incidents related to food adulteration and restrict the illegal food market.
authors.affiliation: a Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation , Ministry of Food and Drug Safety , Cheongju-si , Korea.
authors.forename: Seok
authors.initials: S
authors.lastname: Heo
authors.affiliation: a Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation , Ministry of Food and Drug Safety , Cheongju-si , Korea.
authors.forename: Geum Joo
authors.initials: GJ
authors.lastname: Yoo
authors.affiliation: a Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation , Ministry of Food and Drug Safety , Cheongju-si , Korea.
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Choi
authors.affiliation: a Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation , Ministry of Food and Drug Safety , Cheongju-si , Korea.
authors.forename: Hyoung Joon
authors.initials: HJ
authors.lastname: Park
authors.affiliation: a Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation , Ministry of Food and Drug Safety , Cheongju-si , Korea.
authors.forename: Sung-Kwan
authors.initials: SK
authors.lastname: Park
authors.affiliation: a Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation , Ministry of Food and Drug Safety , Cheongju-si , Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Baek
mesh_terms: D000959:Antihypertensive Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D013997:Time Factors
chemical_list: D000959:Antihypertensive Agents
keywords: Adulteration; anti-hypertensive; dietary supplement; monitoring; ultra-performance liquid chromatography; validation