title: The Effect of Noscapine on Oxygen-Glucose Deprivation on Primary Murine Cortical Neurons in High Glucose Condition.
abstract: In the present work we set out to investigate the neuroprotective effects of noscapine (0.5-2 µM) in presence of D-glucose on primary murine foetal cortical neurons after oxygen-glucose deprivation/24 h. recovery. Cell viability, nitric oxide production and intracellular calcium ((ca(2+))i) levels were evaluated by MTT assay, the modified Griess method and Fura-2 respectively. 25 and 100 mM D-glucose could, in a concentration dependent manner, improve cell viability and decrease NO production and (ca(2+))i level in neuronal cells after ischemic insult. Moreover, pre-incubation of cells with noscapine, noticeably enhanced protective effects of 25 and 100 mM D-glucose compared to similar conditions without noscapine pre-treatment. In fact, noscapine attenuated NO production in a dose-dependent fashion, after 30 minutes (min) OGD, during high-glucose (HG) condition in cortical neurons. Pretreatment with 2 μM noscapine and 25 or 100 mM D-glucose, was shown to decrease the rise in (ca(2+))i induced by Sodium azide/glucose deprivation (chemical OGD) model. These effects were more pronounced than that of 25 or 100 mM D-glucose alone. The present study demonstrated that the neuroprotective effects of HG before an ischemic insult were augmented by pre-treatment with noscapine. Our results also suggested that the neuroprotection offered by both HG and noscapine involve attenuation of NO production and (ca(2+))i levels stimulated by the experimental ischemia in cortical neurons.
authors.affiliation: Cellular and Molecular Research Center (CMRC), Iran University of Medical Sciences, Tehran, Iran. ; Department of Pharmacology, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Gelareh
authors.initials: G
authors.lastname: Vahabzadeh
authors.affiliation: Department of Pharmacology, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Soltan-Ahmed
authors.initials: SA
authors.lastname: Ebrahimi
authors.affiliation: Department of Pharmacology, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Nahid
authors.initials: N
authors.lastname: Rahbar-Roshandel
authors.affiliation: Department of Pharmacology, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Massoud
authors.initials: M
authors.lastname: Mahmoudian
keywords: D-glucose; NO; Noscapine; Oxygen-Glucose Deprivation; intracellular calcium levels