title: Bioactivity of Ruta graveolens and Satureja montana Essential Oils on Solanum tuberosum Hairy Roots and Solanum tuberosum Hairy Roots with Meloidogyne chitwoodi Co-cultures.
abstract: As a nematotoxics screening biotechnological system, Solanum tuberosum hairy roots (StHR) and S. tuberosum hairy roots with Meloidogyne chitwoodi co-cultures (StHR/CRKN) were evaluated, with and without the addition of the essential oils (EOs) of Satureja montana and Ruta graveolens. EOs nematotoxic and phytotoxic effects were followed weekly by evaluating nematode population density in the co-cultures as well as growth and volatile profiles of both in vitro cultures types. Growth, measured by the dissimilation method and by fresh and dry weight determination, was inhibited after EO addition. Nematode population increased in control cultures, while in EO-added cultures numbers were kept stable. In addition to each of the EOs main components, and in vitro cultures constitutive volatiles, new volatiles were detected by gas chromatography and gas chromatography coupled to mass spectrometry in both culture types. StHR with CRKN co-cultures showed to be suitable for preliminary assessment of nematotoxic EOs.
authors.affiliation: Centro de Estudos do Ambiente e do Mar Lisboa, Faculdade de Ciências, Universidade de Lisboa, CBV , C2, Piso 1, Campo Grande, 1749-016 Lisboa, Portugal.
authors.forename: Jorge M S
authors.initials: JM
authors.lastname: Faria
authors.affiliation: Centro de Estudos do Ambiente e do Mar Lisboa, Faculdade de Ciências, Universidade de Lisboa, CBV , C2, Piso 1, Campo Grande, 1749-016 Lisboa, Portugal.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Rodrigues
authors.affiliation: Centro de Estudos do Ambiente e do Mar Lisboa, Faculdade de Ciências, Universidade de Lisboa, CBV , C2, Piso 1, Campo Grande, 1749-016 Lisboa, Portugal.
authors.forename: Inês
authors.initials: I
authors.lastname: Sena
authors.affiliation: Centro de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa , Campo Grande, 1749-016, Lisboa, Portugal.
authors.forename: Cristina
authors.initials: C
authors.lastname: Moiteiro
authors.affiliation: Universidade de Trás-os-Montes e Alto Douro , Quinta dos Prados, Apartado 1013, 5000-911 Vila Real, Portugal.
authors.forename: Richard N
authors.initials: RN
authors.lastname: Bennett
authors.affiliation: NemaLab, ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora , Núcleo da Mitra, Apartado 94, 7002-554 Évora, Portugal.|Departamento de Ciências da Vida, Universidade Lusófona de Humanidades e Tecnologias, EPCV , Campo Grande 376, 1749-024 Lisboa, Portugal.
authors.forename: Manuel
authors.initials: M
authors.lastname: Mota
authors.affiliation: Centro de Estudos do Ambiente e do Mar Lisboa, Faculdade de Ciências, Universidade de Lisboa, CBV , C2, Piso 1, Campo Grande, 1749-016 Lisboa, Portugal.
authors.forename: A Cristina
authors.initials: AC
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D018920:Coculture Techniques; D008401:Gas Chromatography-Mass Spectrometry; D009822:Oils, Volatile / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development / Q000469:parasitology; D032105:Ruta / Q000737:chemistry*; D031362:Satureja / Q000737:chemistry*; D011198:Solanum tuberosum / Q000166:cytology / Q000187:drug effects / Q000469:parasitology*; D014431:Tylenchoidea / Q000187:drug effects / Q000472:pathogenicity*
chemical_list: D009822:Oils, Volatile
keywords: 8-phenyl-2-octanol; Columbia root-knot nematode; biotransformation; in vitro co-cultures; rue; winter savory