title: Synthesis, characterization, and determination of genotoxic effect of a novel dimeric 8-hydroxyquinoline Cd(II) SCN complex.
abstract: In this study, a Cd(II) complex was synthesized using 8-hydroxyquinoline and thiocyanate as the ligands and structurally characterized with the combination of FTIR,1H-NMR,13C-NMR, UV-vis, and MS spectral data. Then, genotoxic effects of the prepared complex were investigated. Genotoxic properties of the dimeric 8-hydroxyquinolinthiocyanatoCd(II) [Cd2(8Q)2(SCN)2] complex synthesized as drug raw material were analyzed in human peripheral blood lymphocytes. Concentrations of 1, 2, 4, 6, and 8 μg/mL [Cd2(8Q)2(SCN)2] were used for 24 and 48  h durations. [Cd2(8Q)2(SCN)2] significantly increased chromosomal aberrations (CAs) at 4, 6, and 8 μg/mL concentrations after a 24- h period and 2 and 4 μg/mL after a 48-h period. [Cd2(8Q)2(SCN)2] significantly decreased the mitotic index (MI) at all concentrations, both at 24 and 48 h. Micronuclei frequency (MN) was not affected by [Cd2(8Q)2(SCN)2] treatment compared with the control. After application for a 48 h period, 6 and 8 μg/mL concentrations showed toxic effects both in chromosomal abnormality and in micronucleus tests. It also decreased the cytokinesis-block proliferation index (CBPI), but this result was statistically significant only at 6 and 8 μg/mL concentrations. In the comet assay (single-cell gel electrophoresis (SCGE)), significant increases in comet tail length, tail moment, and tail intensity were observed at all concentrations. [Cd2(8Q)2(SCN)2] displays clastogenic effect in the concentrations used in human peripheral lymphocytes at chromosomal abnormality, micronucleus tests, and cytokinesis-block proliferation index parameters. Further studies should be conducted in other test systems to evaluate the complete genotoxic potential of [Cd2(8Q)2(SCN)2].
authors.affiliation: a Department of Biology , Faculty of Arts and Sciences, Sakarya University , Esentepe Campus , Sakarya , Turkey and.
authors.forename: Hatice
authors.initials: H
authors.lastname: Tunca
authors.affiliation: a Department of Biology , Faculty of Arts and Sciences, Sakarya University , Esentepe Campus , Sakarya , Turkey and.
authors.forename: Ahmet Ali
authors.initials: AA
authors.lastname: Berber
authors.affiliation: b Department of Chemistry , Faculty of Arts and Sciences, Sakarya University , Esentepe Campus , Sakarya , Turkey.
authors.forename: Kubra
authors.initials: K
authors.lastname: Çanakçi
authors.affiliation: b Department of Chemistry , Faculty of Arts and Sciences, Sakarya University , Esentepe Campus , Sakarya , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Tuna
authors.affiliation: b Department of Chemistry , Faculty of Arts and Sciences, Sakarya University , Esentepe Campus , Sakarya , Turkey.
authors.forename: Salih Zeki
authors.initials: SZ
authors.lastname: Yildiz
authors.affiliation: a Department of Biology , Faculty of Arts and Sciences, Sakarya University , Esentepe Campus , Sakarya , Turkey and.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Aksoy
mesh_terms: D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000139:chemically induced* / Q000706:statistics & numerical data; D020552:Comet Assay; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced / Q000706:statistics & numerical data; D015162:Micronucleus Tests; D008940:Mitotic Index; D009153:Mutagens / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity
chemical_list: D056831:Coordination Complexes; D009153:Mutagens; C000622233:dimeric 8-hydroxyquinolinthiocyanatocadmium(II)
keywords: Dimeric 8-hydroxyquinolinthiocyanatoCd(II) complex; chromosomal aberration; comet assay; cytotoxicity; genotoxicity; micronucleus