title: Insight into the effects of different cropping systems on soil bacterial community and tobacco bacterial wilt rate.
abstract: Rotation is an effective strategy to control crop disease and improve plant health. However, the effects of crop rotation on soil bacterial community composition and structure, and crop health remain unclear. In this study, using 16S rRNA gene sequencing, we explored the soil bacterial communities under four different cropping systems, continuous tobacco cropping (control group), tobacco-maize rotation, tobacco-lily rotation, and tobacco-turnip rotation. Results of detrended correspondence analysis and dissimilarity tests showed that soil bacterial community composition and structure changed significantly among the four groups, such that Acidobacteria and Actinobacteria were more abundant in the maize rotation group (16.6 and 11.5%, respectively) than in the control (8.5 and 7.1%, respectively). Compared with the control group (57.78%), maize and lily were effective rotation crops in controlling tobacco bacterial wilt (about 23.54 and 48.67%). On the other hand, tobacco bacterial wilt rate was increased in the turnip rotation (59.62%) relative to the control. Further study revealed that the abundances of several bacterial populations were directly correlated with tobacco bacterial wilt. For example, Acidobacteria and Actinobacteria were significantly negatively correlated to the tobacco bacterial wilt rate, so they may be probiotic bacteria. Canonical correspondence analysis showed that soil pH and calcium content were key factors in determining soil bacterial communities. In conclusion, our study revealed the composition and structure of bacterial communities under four different cropping systems and may unveil molecular mechanisms for the interactions between soil microorganisms and crop health.
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, China.|Key laboratory of Biometallurgy, Ministry of Education, Changsha, 410083, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Niu
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, 410128, China.|Tobacco Monopoly Bureau of Xiangxi Autonomous Prefecture, Jishou, 416000, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chao
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, China.|Key laboratory of Biometallurgy, Ministry of Education, Changsha, 410083, China.
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, 410128, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, 410128, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, China.|Key laboratory of Biometallurgy, Ministry of Education, Changsha, 410083, China.
authors.forename: Xueduan
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, 410128, China.
authors.forename: Zhongwen
authors.initials: Z
authors.lastname: Rang
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, China.|Key laboratory of Biometallurgy, Ministry of Education, Changsha, 410083, China.
authors.forename: Huaqun
authors.initials: H
authors.lastname: Yin
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, 410128, China.
authors.forename: Linjian
authors.initials: L
authors.lastname: Dai
mesh_terms: D061271:Acidobacteria / Q000235:genetics / Q000302:isolation & purification; D039903:Actinobacteria / Q000235:genetics / Q000302:isolation & purification; D000383:Agriculture / Q000379:methods; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D029688:Brassica napus / Q000254:growth & development; D002118:Calcium; D018556:Crops, Agricultural / Q000254:growth & development*; D006863:Hydrogen-Ion Concentration; D059013:Microbial Consortia / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D019936:Probiotics; D012336:RNA, Ribosomal, 16S; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014026:Nicotiana / Q000254:growth & development / Q000382:microbiology*; D003313:Zea mays / Q000254:growth & development
chemical_list: D012336:RNA, Ribosomal, 16S; D012987:Soil; D002118:Calcium
keywords: 16S rRNA gene sequencing; Crop rotation; Soil bacterial communities; Tobacco bacterial wilt rate