title: MT1-MMP and its potential role in the vertebrate intestinal morphogenesis.
abstract: Membrane type 1-matrix metalloproteinase (MT1-MMP) is involved in numerous biological processes, including morphogenesis. However, the role of MT1-MMP in the development of the vertebrate intestine is poorly understood. This study aimed to evaluate the expression of MT1-MMP in the intestine of rats and chickens along the embryonic and postnatal periods using immunohistochemistry. Results revealed a remarkable spatiotemporal correlation between MT1-MMP expression and intestinal villi morphogenesis in both vertebrates. However, the villi morphogenesis process was found to be different in chickens to that of rats. Moreover, extensive MT1-MMP labeling was observed in the entire villus epithelium from birth until the complete maturation of the small intestinal mucosa in both vertebrates. From these results, we suggest that MT1-MMP contributes to intestinal development, particularly to villi morphogenesis, in both vertebrates. However, further studies are necessary to confirm the role of MT1-MMP in this cellular process. In addition, we performed validation of the primary antibody against human MT1-MMP for adult chickens.
authors.affiliation: Department of Structural and Molecular Biology and Genetic. Electronic address: kkamilla@bol.com.br.
authors.forename: K C
authors.initials: KC
authors.lastname: Camargo
authors.affiliation: Department of Structural and Molecular Biology and Genetic. Electronic address: osteoblasto@ig.com.br.
authors.forename: J R
authors.initials: JR
authors.lastname: Gomes
authors.affiliation: Department of Zootecny, University of Ponta Grossa, Avenida Carlos Cavalcanti, 4748 - Campus Uvaranas - Uvaranas - Ponta Grossa, PR, CEP 84030-900, Brazil. Electronic address: loddimm@yahoo.com.br.
authors.forename: M M
authors.initials: MM
authors.lastname: Loddi
authors.affiliation: Department of Structural and Molecular Biology and Genetic. Electronic address: reginadesordi@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: de Sordi
authors.affiliation: Department of Structural and Molecular Biology and Genetic. Electronic address: crayub@uol.com.br.
authors.forename: C L S
authors.initials: CL
authors.lastname: Costa-Ayub
authors.affiliation: Department of Structural and Molecular Biology and Genetic. Electronic address: tina@uepg.br.
authors.forename: M A de M
authors.initials: MA
authors.lastname: Soares
mesh_terms: D000818:Animals; D002645:Chickens; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D007422:Intestines / Q000166:cytology / Q000196:embryology* / Q000254:growth & development*; D008297:Male; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism*; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured; D014714:Vertebrates / Q000378:metabolism*
chemical_list: D053511:Matrix Metalloproteinase 14
keywords: Development; Intestinal mucosa; Metalloproteinases