title: Simulation of intracellular Ca²⁺ transients in osteoblasts induced by fluid shear stress and its application.
abstract: Intracellular [Formula: see text] transient induced by fluid shear stress (FSS) plays an important role in mechanical regulation of osteoblasts, but the cellular mechanism remains incompletely understood. Here, we constructed a mathematical model combined with experiments to elucidate it. Our simulated and experimental results showed that it was the delay of membrane potential repolarization to produce the refractory period of FSS-induced intracellular calcium transients in osteoblasts. Moreover, the results also demonstrated that the amplitude of FSS-induced intracellular calcium transient is crucial to the proliferation, while its duration is critical to the differentiation, of osteoblasts. Overall, the present study provides a way to understand the cellular mechanism of intracellular calcium transients in osteoblast induced by FSS and explains some of related physiological events.
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
authors.forename: Junqing
authors.initials: J
authors.lastname: Sun
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Xie
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Shi
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
authors.forename: Liyin
authors.initials: L
authors.lastname: Yu
authors.affiliation: Institute of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China. zhangjb@mail.xjtu.edu.cn.
authors.forename: Jianbao
authors.initials: J
authors.lastname: Zhang
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D003198:Computer Simulation; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D008954:Models, Biological; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D013314:Stress, Mechanical
chemical_list: D002118:Calcium
keywords: Fluid shear stress; Mathematical model; Osteoblasts; Refractory period; transient