title: Selected azaphenothiazines inhibit delayed type hypersensitivity and carrageenan reaction in mice.
abstract: Several, previously selected azaphenothiazines, as strongly antiproliferative agents in in vitro models, were subjected to evaluation for their potential immunosuppressive effects in the model of delayed type hypersensitivity (DTH) to ovalbumin (OVA) in BALB/c mice and in foot pad inflammation induced by carrageenan in CBA mice. In the DTH model the compounds were given to mice intraperitoneally (i.p.) in 50μg or 250μg doses, 1h before the elicitation of the response. In the carrageenan-induced foot pad inflammation the compounds were given i.p. in 50μg or 250μg doses, 24h and 2h before administration of carrageenan. Among the compounds, the significantly suppressive activities in both models were exhibited only by compound 5 (6-chloroethylureidoethyldiquino[3,2-b;2',3'-e][1,4]thiazine) and compound 4 (6-acetylaminobutyl-9-chloroquino[3,2-b]benzo[1,4]thiazine). Structure-activity relationship, plausible mechanism of action and potential application in therapy of the compounds are discussed.
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Artym
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
authors.forename: Iwona E
authors.initials: IE
authors.lastname: Kochanowska
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
authors.forename: Maja
authors.initials: M
authors.lastname: Kocięba
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Zaczyńska
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland. Electronic address: zimecki@iitd.pan.wroc.pl.
authors.forename: Michał
authors.initials: M
authors.lastname: Zimecki
authors.affiliation: The Medical University of Silesia, School of Pharmacy with the Division of Laboratory Medicine, Department of Organic Chemistry, Jagiellońska 4, 41-200 Sosnowiec, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Jeleń
authors.affiliation: The Medical University of Silesia, School of Pharmacy with the Division of Laboratory Medicine, Department of Organic Chemistry, Jagiellońska 4, 41-200 Sosnowiec, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Morak-Młodawska
authors.affiliation: The Medical University of Silesia, School of Pharmacy with the Division of Laboratory Medicine, Department of Organic Chemistry, Jagiellońska 4, 41-200 Sosnowiec, Poland.
authors.forename: Krystian
authors.initials: K
authors.lastname: Pluta
mesh_terms: D000485:Allergens; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002351:Carrageenan; D005260:Female; D006968:Hypersensitivity, Delayed / Q000188:drug therapy*; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D010047:Ovalbumin; D010640:Phenothiazines / Q000627:therapeutic use*
chemical_list: D000485:Allergens; D000893:Anti-Inflammatory Agents; D010640:Phenothiazines; D002351:Carrageenan; D010047:Ovalbumin
keywords: Azaphenothiazines; Carrageenan reaction; Delayed type hypersensitivity; Mice