title: Expression of Interactive Genes Associated with Apoptosis and Their Prognostic Value for Ovarian Serous Adenocarcinoma.
abstract: BACKGROUND
  Malignant ovarian tumor is one of the leading causes of worldwide cancer death. It is usually characterized by insidious onset and late diagnosis because of the absence of symptoms, allowing ovarian cancer cases to progress rapidly and become unresectable. The tumor suppressor, p53, plays an important role in regulating cell cycles and apoptosis. p53 is regulated by several molecules, and it interacts with other apoptotic proteins.
  
  
  OBJECTIVE
  To compare the prognosis of ovarian serous carcinoma and evaluate the expression of DNA-PKcs, Akt3, GSK-3β, and p53 in cancerous cells.
  
  
  METHODS
  DNA-PKcs, Akt3, GSK-3β, and p53 expression levels were scored using immunohistochemistry staining of tissue samples from 132 women with ovarian serous adenocarcinoma. Expression was confirmed by real-time RT-PCR. Analyses were stratified by age, tumor grades, cancer stages and serum CA 125 levels.
  
  
  RESULTS
  Significant differences in DNA-PKcs, Akt3, and p53 expression were observed between participants with different stages and tumor grades of ovarian serous adenocarcinoma. DNA-PKcs and p53 expression increased along with increasing tumor grade. Meanwhile, DNA-PKcs, Akt3, and p53 expression increased along with increasing cancer stage, and with a decrease in 5-year overall survival rate.
  
  
  CONCLUSIONS
  This study shows that elevated expression of DNA-PKcs, Akt3, and p53 in ovarian serous adenocarcinoma tissues are an indication of more advanced disease and worse prognosis.
authors.affiliation: Department of Medicine, Pusan National University School of Medicine, Busan, Korea.
authors.forename: Kyusik
authors.initials: K
authors.lastname: Shin
authors.affiliation: Department of Obstetrics and Gynecology, Pusan National University School of Medicine, Biomedical Research Institute and Pusan Cancer Center, Pusan National University Hospital, Busan, Korea.
authors.forename: Ki Hyung
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, Pusan National University School of Medicine, Biomedical Research Institute and Pusan Cancer Center, Pusan National University Hospital, Busan, Korea.
authors.forename: Man Soo
authors.initials: MS
authors.lastname: Yoon
authors.affiliation: Department of Obstetrics and Gynecology, Pusan National University School of Medicine, Biomedical Research Institute and Pusan Cancer Center, Pusan National University Hospital, Busan, Korea.
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Suh
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Konkuk University, Seoul, Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Wonkwang Medical Science, College of Medicine, Wonkwang University, Iksan, Korea.
authors.forename: Ari
authors.initials: A
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Kyunghee University, Seoul, Korea.
authors.forename: Wankyu
authors.initials: W
authors.lastname: Eo
mesh_terms: D017209:Apoptosis; D018394:CA-125 Antigen / Q000097:blood; D018284:Cystadenocarcinoma, Serous / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051747:DNA-Activated Protein Kinase / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D000071679:Glycogen Synthase Kinase 3 beta / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D018394:CA-125 Antigen; D009687:Nuclear Proteins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C491754:AKT3 protein, human; D051747:DNA-Activated Protein Kinase; D000071679:Glycogen Synthase Kinase 3 beta; C497263:PRKDC protein, human; D051057:Proto-Oncogene Proteins c-akt
keywords: Akt3; DNA-PKcs; GSK-3β; epithelial ovarian cancer