title: The Protective Effect of Adalimumab on Renal Injury in a Model of Abdominal Aorta Cross-Clamping.
abstract: BACKGROUND
  Adalimumab (ADA) is a potent inhibitor of tumor necrosis factor (TNF-α). ADA treatment suppresses proinflammatory cytokines, leading to a decrease or inhibition of the inflammatory process.
  
  
  OBJECTIVE
  The aim of this study was to investigate the possible protective effects of ADA on oxidative stress and cellular damage on rat kidney tissue after ischemia/reperfusion (I/R).
  
  
  METHODS
  A total of 30 male Wistar albino rats were divided into three groups: control, I/R, and I/R plus ADA (I/R + ADA); each group comprised 10 animals. The control group underwent laparotomy without I/R injury. After undergoing laparotomy, I/R groups underwent two hours of infrarenal abdominal aortic cross ligation, which was followed by two hours of reperfusion. ADA (50 mg/kg) was administered as a single dose, intraperitoneally, to the I/R + ADA group, 5 days before I/R.
  
  
  RESULTS
  The I/R group's TNF-α (1150.9 ± 145.6 pg/mg protein), IL-1β (287.0 ± 32.4 pg/mg protein) and IL-6 (1085.6 ± 56.7 pg/mg protein) levels were significantly higher than those of the control (916.1 ± 88.7 pg/mg protein, p = 0.003; 187.5 ± 37.2 pg/mg protein, p < 0.001; 881.4 ± 57.1 pg/mg protein, p < 0.001, respectively) and I/R + ADA groups (864.2 ± 169.4 pg/mg protein, p = 0.003; 241.4 ± 33.4 pg/mg protein, p = 0.010; 987.7 ± 66.5 pg/mg protein, p = 0.004, respectively). To date, a few histopathological changes have been reported regarding renal I/R injury in rats due to ADA treatment whereas I/R caused severe histopathological injury to kidney tissue.
  
  
  CONCLUSIONS
  ADA treatment significantly attenuated the severity of kidney I/R injury, inhibiting I/R-induced oxidative stress and renal damage. Because of its anti-inflammatory and antioxidant effects, ADA pretreatment may have protective effects on experimental kidney injury.
authors.affiliation: Department of Biochemistry, Recep Tayyip Erdogan University, Turkey.
authors.forename: Medine C
authors.initials: MC
authors.lastname: Cure
authors.affiliation: Department of Internal Medicine, Recep Tayyip Erdogan University, Turkey.
authors.forename: Erkan
authors.initials: E
authors.lastname: Cure
authors.affiliation: Department of Histology and Embrylogy, Recep Tayyip Erdogan University, Turkey.
authors.forename: Yildiray
authors.initials: Y
authors.lastname: Kalkan
authors.affiliation: Department of Histology and Embrylogy, Recep Tayyip Erdogan University, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Tumkaya
authors.affiliation: Department of Surgery, Recep Tayyip Erdogan University, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Aydin
authors.affiliation: Department of Biochemistry, Recep Tayyip Erdogan University, Turkey.
authors.forename: Aynur
authors.initials: A
authors.lastname: Kirbas
authors.affiliation: Department of Biochemistry, Recep Tayyip Erdogan University, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Efe
authors.affiliation: Department of Thoracic Surgery, Recep Tayyip Erdogan University, Turkey.
authors.forename: Aysel
authors.initials: A
authors.lastname: Kurt
authors.affiliation: Department of Internal Medicine, Recep Tayyip Erdogan University, Turkey.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Yuce
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000068879:Adalimumab / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001012:Aorta, Abdominal / Q000601:surgery*; D017209:Apoptosis / Q000187:drug effects; D024402:Carbonic Anhydrase II / Q000378:metabolism; D003250:Constriction; D019610:Cytoprotection; D004195:Disease Models, Animal; D018836:Inflammation Mediators / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D018836:Inflammation Mediators; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D024402:Carbonic Anhydrase II; D000068879:Adalimumab
keywords: adalimumab; carbonic anhydrases; ischemia; reperfusion injury; tumor necrosis factor alpha