title: Imaginal Disc Transplantation in Drosophila.
abstract: Since Ephrussi and Beadle introduced imaginal disc transplantation to Drosophila research in 1936, the method played an important part towards a better understanding of disc patterning, tissue regeneration, and reprogramming phenomena like transdetermination. Despite increasing usage of high-throughput approaches towards solving biological problems this classical manual method is still in use for studying disc development in a semi-physiological context. Here we describe in detail a protocol and provide recommendations on the procedure in particular for analyzing the regenerative potential of imaginal disks. The steps consist of disc dissection and fragmentation, transplantation into the larval or adult abdomen, and the recovery of implants from the host abdomen. Additionally, we also describe how to make the special transplantation needle from a glass capillary.
authors.affiliation: Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan. tomonori.katsuyama@gmail.com.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Katsuyama
authors.affiliation: Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zürich, Mattenstrasse 26, 4058, Basel, Switzerland.|Faculty of Science, University of Basel, 4056, Basel, Switzerland.
authors.forename: Renato
authors.initials: R
authors.lastname: Paro
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D004330:Drosophila / Q000235:genetics / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D060227:Imaginal Discs / Q000254:growth & development / Q000637:transplantation*; D007814:Larva / Q000235:genetics / Q000254:growth & development; D008967:Molecular Biology / Q000379:methods*; D012038:Regeneration / Q000235:genetics; D014921:Wings, Animal / Q000254:growth & development / Q000637:transplantation*
keywords: Drosophila imaginal discs; Epimorphic regeneration; Polycomb; Transplantation