title: Utilization of mango kernel oil for the rhamnolipid production by Pseudomonas aeruginosa DR1 towards its application as biocontrol agent.
abstract: Mango kernel oil (MKO), derived from mango kernels, considered to be one of the highly generated agro-industrial waste, is assessed for its use as substrate for sustainable production of rhamnolipids. In the present study, MKO in combination with glucose gave maximum rhamnolipid yield of 2.8g/l which reduced the surface tension of water from 72 to 30mN/m, holding a CMC of 80mg/l and also showed high emulsification activity (73%) with diesel. Cell free broth was found to be stable even at high temperature (autoclaved at 121°C for 30min), pH value (up to pH 12) and salinity (up to 20% NaCl). The LC-MS data showed mono-rhamnolipid to be predominant congener followed by di-rhamnolipid in presence of MKO. Whereas, di-rhamnolipid was abundant when a combination of MKO with glucose was used. The produced rhamnolipid mixture showed good antifungal activity against various phytopathogens.
authors.affiliation: Department of Microbiology, Osmania University, Hyderabad 500 007, India.
authors.forename: K
authors.initials: K
authors.lastname: Sathi Reddy
authors.affiliation: Department of Microbiology, Osmania University, Hyderabad 500 007, India.
authors.forename: M
authors.initials: M
authors.lastname: Yahya Khan
authors.affiliation: Department of Microbiology, Osmania University, Hyderabad 500 007, India.
authors.forename: K
authors.initials: K
authors.lastname: Archana
authors.affiliation: Department of Microbiology, Osmania University, Hyderabad 500 007, India.
authors.forename: M
authors.initials: M
authors.lastname: Gopal Reddy
authors.affiliation: Department of Microbiology, Osmania University, Hyderabad 500 007, India. Electronic address: drhami2009@gmail.com.
authors.forename: Bee
authors.initials: B
authors.lastname: Hameeda
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology; D003651:Decanoates / Q000378:metabolism; D006017:Glycolipids / Q000096:biosynthesis*; D007220:Industrial Waste; D031022:Mangifera / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010938:Plant Oils / Q000737:chemistry* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012210:Rhamnose / Q000031:analogs & derivatives / Q000378:metabolism; D054712:Salinity; D013500:Surface Tension; D013501:Surface-Active Agents / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D003651:Decanoates; D006017:Glycolipids; D007220:Industrial Waste; D010938:Plant Oils; D013501:Surface-Active Agents; C418382:rhamnolipid; C085734:rhamnopyranosyl-3-hydroxydecanoyl-3-hydroxydecanoate; D012210:Rhamnose
keywords: Antifungal activity; Biosurfactant; Mango kernel oil; Pseudomonas aeruginosa; Rhamnolipids