title: Live Imaging of Influenza Infection of the Trachea Reveals Dynamic Regulation of CD8+ T Cell Motility by Antigen.
abstract: During a primary influenza infection, cytotoxic CD8+ T cells need to infiltrate the infected airways and engage virus-infected epithelial cells. The factors that regulate T cell motility in the infected airway tissue are not well known. To more precisely study T cell infiltration of the airways, we developed an experimental model system using the trachea as a site where live imaging can be performed. CD8+ T cell motility was dynamic with marked changes in motility on different days of the infection. In particular, significant changes in average cell velocity and confinement were evident on days 8-10 during which the T cells abruptly but transiently increase velocity on day 9. Experiments to distinguish whether infection itself or antigen affect motility revealed that it is antigen, not active infection per se that likely affects these changes as blockade of peptide/MHC resulted in increased velocity. These observations demonstrate that influenza tracheitis provides a robust experimental foundation to study molecular regulation of T cell motility during acute virus infection.
authors.affiliation: David H. Smith Center for Vaccine Biology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.|Department of Microbiology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.
authors.forename: Kris
authors.initials: K
authors.lastname: Lambert Emo
authors.affiliation: David H. Smith Center for Vaccine Biology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.|Department of Microbiology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Hyun
authors.affiliation: David H. Smith Center for Vaccine Biology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.|Department of Microbiology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.
authors.forename: Emma
authors.identifier: 0000-0003-1619-085X
authors.initials: E
authors.lastname: Reilly
authors.affiliation: David H. Smith Center for Vaccine Biology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.|Department of Microbiology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.
authors.forename: Christopher
authors.initials: C
authors.lastname: Barilla
authors.affiliation: Department of Surgery, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.
authors.forename: Scott
authors.initials: S
authors.lastname: Gerber
authors.affiliation: David H. Smith Center for Vaccine Biology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.|Department of Microbiology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.
authors.forename: Deborah
authors.initials: D
authors.lastname: Fowell
authors.affiliation: David H. Smith Center for Vaccine Biology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.|Department of Microbiology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.
authors.forename: Minsoo
authors.initials: M
authors.lastname: Kim
authors.affiliation: David H. Smith Center for Vaccine Biology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.|Department of Microbiology & Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.
authors.forename: David J
authors.identifier: 0000-0002-9435-8673
authors.initials: DJ
authors.lastname: Topham
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D002465:Cell Movement; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000276:immunology / Q000821:virology; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000473:pathology / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000821:virology; D014132:Trachea / Q000276:immunology / Q000473:pathology / Q000821:virology; D014136:Tracheitis / Q000276:immunology* / Q000473:pathology / Q000821:virology
chemical_list: D000956:Antigens, Viral