title: Design of a stable cell line producing a recombinant monoclonal anti-TNFα antibody based on a CHO cell line.
abstract: Recombinant monoclonal antibodies (mAbs) against tumor necrosis factor alpha are widely used in the biopharmaceutical therapy of autoimmune diseases. Currently, a large number of drugs based on these antibodies are available. Accordingly, the development of these products for the Russian market is an important goal. The aim of the current study is to describe the development of one such technology. CHO-DG44-derived cell lines producing mAb were developed using two strategies, one based on individual clones and the other based on cell pools. To obtain recombinant cell lines with highly amplified genes of interest, the clones underwent dihydrofolate reductase-mediated gene amplification. Using the best strategy for the selection and amplification of mAb-producing clones, we achieved the production of more than 1 g/L in small scale, non-optimized conditions.
authors.affiliation: PHARMAPARK LLC, Bldg. 1, 8 Nauchny proezd, Moscow, Russian Federation.
authors.forename: E V
authors.initials: EV
authors.lastname: Voronina
authors.affiliation: PHARMAPARK LLC, Bldg. 1, 8 Nauchny proezd, Moscow, Russian Federation.
authors.forename: Y A
authors.initials: YA
authors.lastname: Seregin
authors.affiliation: PHARMAPARK LLC, Bldg. 1, 8 Nauchny proezd, Moscow, Russian Federation.
authors.forename: N A
authors.initials: NA
authors.lastname: Litvinova
authors.affiliation: M.V. Lomonosov Moscow State Academy of Fine Chemical Technology, Moscow, Russian Federation.
authors.forename: V I
authors.initials: VI
authors.lastname: Shvets
authors.affiliation: PHARMAPARK LLC, Bldg. 1, 8 Nauchny proezd, Moscow, Russian Federation.
authors.forename: R R
authors.initials: RR
authors.lastname: Shukurov
keywords: CHO-DG44; Dihydrofolate reductase mediated gene amplification; Monoclonal anti-TNFα antibody; Tumor necrosis factor alpha