title: Multivariate analysis of the volatile components in tobacco based on infrared-assisted extraction coupled to headspace solid-phase microextraction and gas chromatography-mass spectrometry.
abstract: A novel infrared-assisted extraction coupled to headspace solid-phase microextraction followed by gas chromatography with mass spectrometry method has been developed for the rapid determination of the volatile components in tobacco. The optimal extraction conditions for maximizing the extraction efficiency were as follows: 65 μm polydimethylsiloxane-divinylbenzene fiber, extraction time of 20 min, infrared power of 175 W, and distance between the infrared lamp and the headspace vial of 2 cm. Under the optimum conditions, 50 components were found to exist in all ten tobacco samples from different geographical origins. Compared with conventional water-bath heating and nonheating extraction methods, the extraction efficiency of infrared-assisted extraction was greatly improved. Furthermore, multivariate analysis including principal component analysis, hierarchical cluster analysis, and similarity analysis were performed to evaluate the chemical information of these samples and divided them into three classifications, including rich, moderate, and fresh flavors. The above-mentioned classification results were consistent with the sensory evaluation, which was pivotal and meaningful for tobacco discrimination. As a simple, fast, cost-effective, and highly efficient method, the infrared-assisted extraction coupled to headspace solid-phase microextraction technique is powerful and promising for distinguishing the geographical origins of the tobacco samples coupled to suitable chemometrics.
authors.affiliation: Technology Center, China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou, P. R. China.|Department of Chemistry, Zhejiang University, Hangzhou, P. R. China.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou, P. R. China.
authors.forename: Yuanjiang
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Technology Center, China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou, P. R. China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Technology Center, China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou, P. R. China.
authors.forename: Guohai
authors.initials: G
authors.lastname: Chu
authors.affiliation: Technology Center, China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Technology Center, China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou, P. R. China.
authors.forename: Kailong
authors.initials: K
authors.lastname: Yuan
authors.affiliation: Technology Center, China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou, P. R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xia
authors.affiliation: Technology Center, China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou, P. R. China.
authors.forename: Changhe
authors.initials: C
authors.lastname: Cheng
mesh_terms: D004129:Dimethylpolysiloxanes; D008401:Gas Chromatography-Mass Spectrometry; D015999:Multivariate Analysis; D064209:Phytochemicals / Q000032:analysis*; D011145:Polyvinyls; D052617:Solid Phase Microextraction; D014026:Nicotiana / Q000737:chemistry*; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D004129:Dimethylpolysiloxanes; D064209:Phytochemicals; D011145:Polyvinyls; D055549:Volatile Organic Compounds; C445089:poly(dimethylsiloxane-divinylbenzene)
keywords: Chemometrics; Gas chromatography with mass spectrometry; Headspace solid-phase microextraction; Infrared-assisted extraction; Tobacco