title: NbCZF1, a Novel C2H2-Type Zinc Finger Protein, as a New Regulator of SsCut-Induced Plant Immunity in Nicotiana benthamiana.
abstract: SsCut, which functions as an elicitor, can induce plant immunity. In this study, we utilized Nicotiana benthamiana and virus-induced gene silencing to decrease the expression of > 2,500 genes individually. Using this forward genetics approach, several genes were identified that, when silenced, compromised SsCut-triggered cell death based on a cell death assay. A C2H2-type zinc finger gene was isolated from N. benthamiana Sequence analysis indicated that the gene encodes a 27 kDa protein with 253 amino acids containing two typical C2H2-type zinc finger domains; this gene was named NbCZF1 We found that SsCut-induced cell death could be inhibited by virus-induced gene silencing of NbCZF1 in N. benthamiana In addition, SsCut induces stomatal closure, accompanied by reactive oxygen species (ROS) production by NADPH oxidases and nitric oxide (NO) production. NbCZF1-silenced plants showed impaired SsCut-induced stomatal closure, decreased SsCut-induced production of ROS and NO in guard cells and reduced SsCut-induced resistance against Phytophthora nicotianae Taken together, these results demonstrate that the NbCZF1-ROS-NO pathway mediates multiple SsCut-triggered responses, including stomatal closure, hypersensitive responses and defense-related gene expression. This is the first report describing the function of a C2H2-type zinc finger protein in N. benthamiana.
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei, 230036, China hjzhang@ahau.edu.cn gaozhimou@126.com.|These authors contributed equally to this work.
authors.forename: Huajian
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei, 230036, China.|These authors contributed equally to this work.
authors.forename: Tongyao
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei, 230036, China.
authors.forename: Peitong
authors.initials: P
authors.lastname: Zhuang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei, 230036, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei, 230036, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Du
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei, 230036, China.
authors.forename: Zhaozhao
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei, 230036, China hjzhang@ahau.edu.cn gaozhimou@126.com.
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Gao
mesh_terms: D000595:Amino Acid Sequence; D000070580:CYS2-HIS2 Zinc Fingers; D016923:Cell Death; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D009569:Nitric Oxide / Q000378:metabolism; D010802:Phylogeny; D010838:Phytophthora / Q000502:physiology*; D010935:Plant Diseases / Q000276:immunology*; D057865:Plant Immunity; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics / Q000276:immunology / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D054046:Plant Stomata / Q000166:cytology / Q000235:genetics / Q000276:immunology / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000276:immunology / Q000502:physiology
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D009569:Nitric Oxide
keywords: Elicitor; Hypersensitive response; Plant defense; SsCut; Stomatal closure