title: The Salinity Responsive Mechanism of a Hydroxyproline-Tolerant Mutant of Peanut Based on Digital Gene Expression Profiling Analysis.
abstract: Soil salinity seriously limits plant growth and yield. Strategies have been developed for plants to cope with various environmental stresses during evolution. To screen for the broad-spectrum genes and the molecular mechanism about a hydroxyproline-tolerant mutant of peanut with enhanced salinity resistance under salinity stress, digital gene expression (DGE) sequencing was performed in the leaves of salinity-resistant mutant (S2) and Huayu20 as control (S4) under salt stress. The results indicate that major transcription factor families linked to salinity stress responses (NAC, bHLH, WRKY, AP2/ERF) are differentially expressed in the leaves of peanut under salinity stress. In addition, genes related to cell wall loosening and stiffening (xyloglucan endotransglucosylase/hydrolases, peroxidases, lipid transfer protein, expansin, extension), late embryogenesis abundant protein family, fatty acid biosynthesis and metabolism (13-lipoxygenase omega-6 fatty acid desaturase, omega-3 fatty acid desaturase) and some previously reported stress-related genes encoding proteins such as defensin, universal stress protein, metallothionein, peroxidase etc, and some other known or unknown function stress related genes, have been identified. The information from this study will be useful for further research on the mechanism of salinity resistance and will provide a useful genomic resource for the breeding of salinity resistance variety in peanut.
authors.affiliation: College of Life Science, Qingdao Agricultural University, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao 266109, China.
authors.forename: Jiongming
authors.initials: J
authors.lastname: Sui
authors.affiliation: College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Defeng
authors.initials: D
authors.lastname: Jiang
authors.affiliation: College of Life Science, Qingdao Agricultural University, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao 266109, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: College of Life Science, Qingdao Agricultural University, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao 266109, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Song
authors.affiliation: College of Life Science, Qingdao Agricultural University, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao 266109, China.
authors.forename: Jingshan
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Life Science, Qingdao Agricultural University, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao 266109, China.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Life Science, Qingdao Agricultural University, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao 266109, China.
authors.forename: Lixian
authors.initials: L
authors.lastname: Qiao