title: Effect of Cross-Sex Hormonal Replacement on Antioxidant Enzymes in Rat Retroperitoneal Fat Adipocytes.
abstract: We report the effect of cross-sex hormonal replacement on antioxidant enzymes from rat retroperitoneal fat adipocytes. Eight rats of each gender were assigned to each of the following groups: control groups were intact female or male (F and M, resp.). Experimental groups were ovariectomized F (OvxF), castrated M (CasM), OvxF plus testosterone (OvxF + T), and CasM plus estradiol (CasM + E2) groups. After sacrifice, retroperitoneal fat was dissected and processed for histology. Adipocytes were isolated and the following enzymatic activities were determined: Cu-Zn superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR). Also, glutathione (GSH) and lipid peroxidation (LPO) were measured. In OvxF, retroperitoneal fat increased and adipocytes were enlarged, while in CasM rats a decrease in retroperitoneal fat and small adipocytes are observed. The cross-sex hormonal replacement in F rats was associated with larger adipocytes and a further decreased activity of Cu-Zn SOD, CAT, GPx, GST, GR, and GSH, in addition to an increase in LPO. CasM + E2 exhibited the opposite effects showing further activation antioxidant enzymes and decreases in LPO. In conclusion, E2 deficiency favors an increase in retroperitoneal fat and large adipocytes. Cross-sex hormonal replacement in F rats aggravates the condition by inhibiting antioxidant enzymes.
authors.affiliation: Department of Pathology, Instituto Nacional de Cardiología "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, Mexico.
authors.forename: Israel
authors.identifier: 0000-0001-6510-2954
authors.initials: I
authors.lastname: Pérez-Torres
authors.affiliation: Department of Physiology, Instituto Nacional de Cardiología "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, Mexico.
authors.forename: Verónica
authors.identifier: 0000-0002-2655-7590
authors.initials: V
authors.lastname: Guarner-Lans
authors.affiliation: Department of Pathology, Instituto Nacional de Cardiología "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, Mexico.
authors.forename: Alejandra
authors.identifier: 0000-0002-0522-9395
authors.initials: A
authors.lastname: Zúñiga-Muñoz
authors.affiliation: Department of Pathology, Instituto Nacional de Cardiología "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, Mexico.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Velázquez Espejel
authors.affiliation: Department of Molecular Genetic, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Coyoacán, 04510 México City, Mexico.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Cabrera-Orefice
authors.affiliation: Department of Molecular Genetic, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Coyoacán, 04510 México City, Mexico.
authors.forename: Salvador
authors.initials: S
authors.lastname: Uribe-Carvajal
authors.affiliation: Department of Pharmacology, Instituto Nacional de Cardiología "Ignacio Chavez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, Mexico.
authors.forename: Natalia
authors.initials: N
authors.lastname: Pavón
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004958:Estradiol / Q000097:blood / Q000494:pharmacology*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D050152:Intra-Abdominal Fat / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D013739:Testosterone / Q000097:blood / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D016207:Cytokines; D013739:Testosterone; D004958:Estradiol; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione