title: Molecular analysis of lungs from pigs immunized with a mutant transferrin binding protein B-based vaccine and challenged with Haemophilus parasuis.
abstract: The molecular analysis of pigs vaccinated with a mutant transferrin-binding protein B (Y167A) from Haemophilus parasuis was compared with that performed for unvaccinated challenged (UNCH) and unvaccinated unchallenged (UNUN) pigs. Microarray analysis revealed that UNCH group showed the most distinct expression profile for immune response genes, mainly for those genes involved in inflammation or immune cell trafficking. This fact was confirmed by real-time PCR, in which the greatest level of differential expression from this group were CD14, CD163, IL-8 and IL-12. In Y167A group, overexpressed genes included MAP3K8, CD14, IL-12 and CD163. Proteomics revealed that collagen α-1 and peroxiredoxins 2 and 6 were overexpressed in Y167A pigs. Our study reveals new data on genes and proteins involved in H. parasuis infection and several candidates of resistance to infection that are induced by Y167A vaccine. The expression of proinflammatory molecules from Y176A pigs is similar to their expression in UNUN pigs.
authors.affiliation: Department of Animal Health, Faculty of Veterinary Medicine, University of León, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Martínez-Martínez
authors.affiliation: Department of Animal Health, Faculty of Veterinary Medicine, University of León, Spain.
authors.forename: E F
authors.initials: EF
authors.lastname: Rodríguez-Ferri
authors.affiliation: Department of Animal Health, Faculty of Veterinary Medicine, University of León, Spain; Laboratory of Microbiology and Advanced Immunology, Faculty of Agronomy and Veterinary Medicine, University of Passo Fundo, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Frandoloso
authors.affiliation: Department of Genetics, Faculty of Veterinary Medicine, University of Córdoba, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Garrido-Pavón
authors.affiliation: Department of Genetics, Faculty of Veterinary Medicine, University of Córdoba, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Zaldívar-López
authors.affiliation: INBIOTEC (Biotechnology Institute), Scientific Park in León, Avda. Real 1, León, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Barreiro
authors.affiliation: Department of Animal Health, Faculty of Veterinary Medicine, University of León, Spain. Electronic address: cbgutm@unileon.es.
authors.forename: C B
authors.initials: CB
authors.lastname: Gutiérrez-Martín
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D016207:Cytokines / Q000235:genetics; D044137:Haemophilus parasuis / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D007249:Inflammation / Q000235:genetics; D008168:Lung / Q000276:immunology* / Q000382:microbiology; D013058:Mass Spectrometry; D009154:Mutation; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control*; D046888:Tissue Array Analysis; D033882:Transferrin-Binding Protein B / Q000235:genetics / Q000276:immunology*; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D016207:Cytokines; D033882:Transferrin-Binding Protein B; D022223:Vaccines, Subunit
keywords: Glässer’s disease; Haemophilus parasuis; Microarray; Mutant transferrin binding protein B; Proteomics; Quantitative real-time PCR; Subunit vaccine; Vaccination