title: The expression of miRNA-221 and miRNA-222 in gliomas patients and their prognosis.
abstract: The aim of this study is to explore the expression of microRNA (miRNA)-221 and miRNA-222 in human glioma cells and tissues. The expression of miRNA-221 and miRNA-222 in human glioma cell line U87, U251, A172, LN229 and surgery resected glioma tissues were measured. The survival rate of X-ray (2 Gy) irradiated glioma cells were calculated. 165 cases of glioma patients were recruited successfully; the expression of miRNA-221 and miRNA-222 in their resected tissues were measured. The expression of miRNA-221 and miRNA-222 in cancer tissues were obviously higher than control tissues (normal brain tissue) and control cell (gastric mucosal epithelial cell, GES) (p < 0.05). The highly malignant glioma tissues expressed significantly higher miRNA-221 and miRNA-222 than low malignant glioma tissues. Patients with highly expressed miRNA-221 and miRNA-222 have shorter survival time. Survival rate of glioma cells was significantly higher than GES cell after irradiation (p < 0.05); miRNA-221 in glioma cells. The expressions of miRNA-221 and miRNA-222 in irritated glioma cells were positively correlated with the survival rate of glioma cells (r = 0.629, 0.712, both p < 0.01). For the 165 glioma patients, the expressions of miRNA-221 and miRNA-222 increased with the increasing of pathological grades (χ2= 42.85, p < 0.01); and their survival time decreased when miRNA-221 expression elevated (χ2= 57.12, p < 0.01). MiRNA-221 and miRNA-222 express highly in human glioma cells and tissues. Expression of miRNA-221 and miRNA-222 are closely related to pathological grading and prognosis of glioma; they could be used as independent prognostic factor for glioma.
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, 54 Anshan Road, Heping District, Tianjin, 300052, People's Republic of China. xueliang32@126.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Xue
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, 54 Anshan Road, Heping District, Tianjin, 300052, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, 54 Anshan Road, Heping District, Tianjin, 300052, People's Republic of China.
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Yue
authors.affiliation: Department of Neurosurgery, Tianjin Medical University General Hospital, 54 Anshan Road, Heping District, Tianjin, 300052, People's Republic of China.
authors.forename: Jianning
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D011379:Prognosis; D015996:Survival Rate
chemical_list: D035683:MicroRNAs; C525709:MIR221, human; C525710:MIR222, human
keywords: Glioma; Prognostic factor; Radiosensitivity; Survival; miRNA-221; miRNA-222