title: The molecular epidemiology of bovine rotaviruses circulating in Iran: a two-year study.
abstract: Bovine group A rotavirus (bovine RVA) is recognized as a major cause of severe gastroenteritis in newborn calves. The purpose of this study was to estimate the prevalence and identify the genotypes of circulating bovine RVA in newborn diarrheic calves. Two hundred fifty-three stool samples of diarrheic calves up to 1 month old were collected from 42 industrial dairy farms in two Iranian provinces during March 2010 to February 2012. All collected samples were screened for the presence of bovine RVA by RT-PCR, and the G and P genotypes were determined by semi-nested multiplex RT-PCR assay. The results of RT-PCR indicated that 49.4 % (125 out of 253) of the samples were positive for bovine RVA. The G and P genotyping of a subset of positive samples (n = 85) by semi-nested multiplex RT-PCR revealed that G6 (55.3 %) and G10 (43.5 %) and P[5] (51.8 %) and P[11] (27 %) were the most prevalent G and P genotypes, respectively. G6P[5] was the dominant genotype (35.3 %), followed by G10P[5], G10P[11] and G6P[11], with prevalence rates of 16.5 %, 15.3 % and 10.6 %, respectively. Sequence analysis of 20 VP7 and four VP4 genes showed highest nucleotide sequence identity with the corresponding genes of strains RVA/Cow-tc/GBR/UK/1973/G6P7[5] and RVA/Cow-tc/USA/B223/XXXX/G10P[11]. The results of this study reveal the diversity of G and P genotypes in bovine RVA samples from diarrheic Iranian calves and expands our knowledge of bovine RVA infections in the Middle East. These results also highlight the importance of producing of an effective rotavirus vaccine and its inclusion in the national cattle immunization program.
authors.affiliation: Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Iran. farzaneh.pourasgari@gmail.com.|Division of Advanced Diagnostics, Toronto General Research Institute, UHN, Toronto, Canada. farzaneh.pourasgari@gmail.com.
authors.forename: Farzaneh
authors.identifier: http://orcid.org/0000-0002-7989-7732
authors.initials: F
authors.lastname: Pourasgari
authors.affiliation: Laboratory of Virology, National Reference Center for Enteric Viruses, CHU F. Mitterrand, Dijon, France.|AgroSup Dijon, PAM UMR A 02.102, Université de Bourgogne Franche-Comte, Dijon, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Kaplon
authors.affiliation: Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Iran.
authors.forename: Shahla
authors.initials: S
authors.lastname: Karimi-Naghlani
authors.affiliation: Laboratory of Virology, National Reference Center for Enteric Viruses, CHU F. Mitterrand, Dijon, France.|Queen's Elizabeth Hospital, Birmingham, UK.
authors.forename: Céline
authors.initials: C
authors.lastname: Fremy
authors.affiliation: Iran Veterinary Organization, Tehran, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Otarod
authors.affiliation: Laboratory of Virology, National Reference Center for Enteric Viruses, CHU F. Mitterrand, Dijon, France.|AgroSup Dijon, PAM UMR A 02.102, Université de Bourgogne Franche-Comte, Dijon, France.
authors.forename: Katia
authors.initials: K
authors.lastname: Ambert-Balay
authors.affiliation: Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mirjalili
authors.affiliation: Laboratory of Virology, National Reference Center for Enteric Viruses, CHU F. Mitterrand, Dijon, France. pierre.pothier@u-bourgogne.fr.|AgroSup Dijon, PAM UMR A 02.102, Université de Bourgogne Franche-Comte, Dijon, France. pierre.pothier@u-bourgogne.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Pothier
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology*; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D005838:Genotype; D060005:Genotyping Techniques; D007492:Iran / Q000453:epidemiology; D017720:Molecular Epidemiology; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus