title: Dioxinodehydroeckol Enhances the Differentiation of Osteoblasts by Regulating the Expression of Phospho-Smad1/5/8.
abstract: Lack of bone formation-related health problems are a major problem for the aging population in the modern world. As a part of the ongoing trend of developing natural substances that attenuate osteoporotic bone loss conditions, dioxinodehydroeckol (DHE) from edible brown alga Ecklonia cava was tested for its effects on osteoblastogenic differentiation in MC3T3-E1 pre-osteoblasts. DHE was observed to successfully enhance osteoblast differentiation, as indicated by elevated cell proliferation, alkaline phosphatase activity, intracellular cell mineralization, along with raised levels of osteoblastogenesis indicators at the concentration of 20 μM. Results suggested a possible intervening of DHE on the bone morphogenetic protein (BMP) signaling pathway, according to elevated protein levels of BMP-2, collagen-I, and Smads. In addition, the presence of DHE was also able to raise the phosphorylated extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) levels which are also activated by the BMP signaling pathway. In conclusion, DHE is suggested to be a potential bioactive compound against bone loss that could enhance osteoblastogenesis with a suggested BMP pathway interaction.
authors.affiliation: Department of Organic Material Science and Engineering, Pusan National University, Busan 46241, Korea. icetwig@naver.com.
authors.forename: Byul-Nim
authors.initials: BN
authors.lastname: Ahn
authors.affiliation: Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Busan 46958, Korea. karadenizf@outlook.com.|Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Busan 46958, Korea. karadenizf@outlook.com.
authors.forename: Fatih
authors.initials: F
authors.lastname: Karadeniz
authors.affiliation: Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Busan 46958, Korea. cskong@silla.ac.kr.|Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Busan 46958, Korea. cskong@silla.ac.kr.
authors.forename: Chang-Suk
authors.initials: CS
authors.lastname: Kong
authors.affiliation: Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Korea. dennis011@korea.kr.
authors.forename: Ki-Ho
authors.initials: KH
authors.lastname: Nam
authors.affiliation: Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Korea. suni1@korea.kr.
authors.forename: Mi-Soon
authors.initials: MS
authors.lastname: Jang
authors.affiliation: Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University, Busan 49112, Korea. ywseo@kmou.ac.kr.|Department of Convergence Study on the Ocean Science and Technology, Ocean Science and Technology School, Korea Maritime and Ocean University, Busan 49112, Korea. ywseo@kmou.ac.kr.
authors.forename: Youngwan
authors.initials: Y
authors.lastname: Seo
authors.affiliation: Department of Organic Material Science and Engineering, Pusan National University, Busan 46241, Korea. hanseongkim@pusan.ac.kr.
authors.forename: Han Seong
authors.initials: HS
authors.lastname: Kim
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis / Q000235:genetics; D019485:Bone Morphogenetic Proteins / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics; D004147:Dioxins / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D010024:Osteoporosis / Q000517:prevention & control; D000459:Phaeophyceae / Q000737:chemistry; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D051785:Smad Proteins / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D024042:Collagen Type I; D004147:Dioxins; D051785:Smad Proteins; C550453:dioxinodehydroeckol; D012313:RNA; D000469:Alkaline Phosphatase
keywords: BMP-2; Ecklonia cava; Smad; dioxinodehydroeckol; osteoblastogenesis