title: The minimum information required for a glycomics experiment (MIRAGE) project: sample preparation guidelines for reliable reporting of glycomics datasets.
abstract: The minimum information required for a glycomics experiment (MIRAGE) project was established in 2011 to provide guidelines to aid in data reporting from all types of experiments in glycomics research including mass spectrometry (MS), liquid chromatography, glycan arrays, data handling and sample preparation. MIRAGE is a concerted effort of the wider glycomics community that considers the adaptation of reporting guidelines as an important step towards critical evaluation and dissemination of datasets as well as broadening of experimental techniques worldwide. The MIRAGE Commission published reporting guidelines for MS data and here we outline guidelines for sample preparation. The sample preparation guidelines include all aspects of sample generation, purification and modification from biological and/or synthetic carbohydrate material. The application of MIRAGE sample preparation guidelines will lead to improved recording of experimental protocols and reporting of understandable and reproducible glycomics datasets.
authors.affiliation: Department of Biochemistry, Glycobiology Institute , University of Oxford, Oxford OX1 3QU, UK weston.struwe@bioch.ox.ac.uk ckettner@beilstein-institut.de.
authors.forename: Weston B
authors.initials: WB
authors.lastname: Struwe
authors.affiliation: Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Agravat
authors.affiliation: Department of Bioinformatics, Faculty of Engineering, Soka University, 1-236 Tangi-machi, Hachioji, Tokyo 192-8577, Japan.
authors.forename: Kiyoko F
authors.initials: KF
authors.lastname: Aoki-Kinoshita
authors.affiliation: Biomolecular Frontiers Research Centre, Macquarie University, Sydney, NSW 2109, Australia.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Campbell
authors.affiliation: Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University, School of Medicine, 670 Albany Street, Suite 504, Boston, MA 02118, USA.
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Costello
authors.affiliation: Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK.
authors.forename: Anne
authors.initials: A
authors.lastname: Dell
authors.affiliation: Department of Medicine, Glycosciences Laboratory, Imperial College London, Du Cane Road, London W12 0NN, UK.
authors.lastname: Ten Feizi
authors.affiliation: Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK.
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Haslam
authors.affiliation: Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, PO Box 440, 405 30 Gothenburg, Sweden.
authors.forename: Niclas G
authors.initials: NG
authors.lastname: Karlsson
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, 128, Academia Road Sec. 2, Nankang, Taipei 115, Taiwan.
authors.forename: Kay-Hooi
authors.initials: KH
authors.lastname: Khoo
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kolarich
authors.affiliation: Department of Medicine, Glycosciences Laboratory, Imperial College London, Du Cane Road, London W12 0NN, UK.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Cell and Molecular Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: McBride
authors.affiliation: Department of Chemistry, Indiana University, 800 E. Kirkwood Avenue, Bloomington, IN 47405, USA.
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
authors.affiliation: Biomolecular Frontiers Research Centre, Macquarie University, Sydney, NSW 2109, Australia.
authors.forename: Nicolle H
authors.initials: NH
authors.lastname: Packer
authors.affiliation: Department of Cell and Molecular Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems, 39106 Magdeburg, Germany.
authors.forename: Erdmann
authors.initials: E
authors.lastname: Rapp
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
authors.forename: Rene
authors.initials: R
authors.lastname: Ranzinger
authors.affiliation: NIBRT GlycoScience Group, NIBRT-National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, Ireland.
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
authors.affiliation: Center for Comprehensive Informatics, Research and Woodruff Health Sciences IT, Emory University, 1784 N. Decatur Rd, Atlanta, GA 30322, USA.
authors.forename: David F
authors.initials: DF
authors.lastname: Smith
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Tiemeyer
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
authors.forename: Lance
authors.initials: L
authors.lastname: Wells
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
authors.forename: William S
authors.initials: WS
authors.lastname: York
authors.affiliation: Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University, School of Medicine, 670 Albany Street, Suite 504, Boston, MA 02118, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Zaia
authors.affiliation: Beilstein-Institut, Trakehner Str. 7-9, 60487 Frankfurt am Main, Germany weston.struwe@bioch.ox.ac.uk ckettner@beilstein-institut.de.
authors.forename: Carsten
authors.initials: C
authors.lastname: Kettner
mesh_terms: D002853:Chromatography, Liquid; D066264:Datasets as Topic; D054794:Glycomics / Q000379:methods*; D017408:Guidelines as Topic; D013058:Mass Spectrometry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*
chemical_list: D011134:Polysaccharides
keywords: MIRAGE; glycobiology; glycomics; sample preparation