title: NHERF1 in Microvilli of Vomeronasal Sensory Neurons.
abstract: In most mammals, the vomeronasal system detects a variety of (semio)chemicals that mediate olfactory-driven social and sexual behaviors. Vomeronasal chemosensation depends on G protein-coupled receptors (V1R, V2R, and FPR-rs) that operate at remarkably low stimulus concentrations, thus, indicating a highly sensitive and efficient signaling pathway. We identified the PDZ domain-containing protein, Na+/H+exchanger regulatory factor-1 (NHERF1), as putative molecular organizer of signal transduction in vomeronasal neurons. NHERF1 is a protein that contains 2 PDZ domains and a carboxy-terminal ezrin-binding domain. It localizes to microvilli of vomeronasal sensory neurons and interacts with V1Rs. Furthermore, NHERF1 and Gαi2are closely colocalized. These findings open up new aspects of the functional organization and regulation of vomeronasal signal transduction by PDZ scaffolding proteins.
authors.affiliation: Pharmacology and Toxicology, University Hospital Jena, Friedrich Schiller University Jena, Drackendorfer Straße 1, 07743 Jena, Germany.|Cluster of Excellence NeuroCure, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany and.|Present address: Institute of Anatomy, Medical Faculty, University Duisburg-Essen, Hufelandstr. 55, 45147 Essen, Germany.
authors.forename: Bastian
authors.initials: B
authors.lastname: Henkel
authors.affiliation: Cluster of Excellence NeuroCure, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany and.|Present address: Institute of Biochemistry, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Willem
authors.initials: W
authors.lastname: Bintig
authors.affiliation: Pharmacology and Toxicology, University Hospital Jena, Friedrich Schiller University Jena, Drackendorfer Straße 1, 07743 Jena, Germany.
authors.forename: S Sneha
authors.initials: SS
authors.lastname: Bhat
authors.affiliation: Department of Chemosensation, Institute for Biology II, RWTH-Aachen University, Worringerweg 3, 52074 Aachen, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Spehr
authors.affiliation: Pharmacology and Toxicology, University Hospital Jena, Friedrich Schiller University Jena, Drackendorfer Straße 1, 07743 Jena, Germany, eva.neuhaus@med.uni-jena.de.|Cluster of Excellence NeuroCure, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany and.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Neuhaus
mesh_terms: D000818:Animals; D051379:Mice; D008810:Mice, Inbred C57BL; D008871:Microvilli / Q000737:chemistry* / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000737:chemistry* / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D019147:Vomeronasal Organ / Q000166:cytology*
chemical_list: D010750:Phosphoproteins; D017923:Sodium-Hydrogen Exchangers; C093376:sodium-hydrogen exchanger regulatory factor
keywords: NHERF1; PDZ; V1R; microvilli; vomeronasal