title: Molecular Typing of Mycobacterium bovis from Cattle Reared in Midwest Brazil.
abstract: Mycobacterium bovis is the causative agent of bovine tuberculosis (BTB), the pathogen responsible for serious economic impact on the livestock sector. In order to obtain data on isolated M. bovis strains and assist in the control and eradication program for BTB, a cross sectional descriptive molecular epidemiology study in the Brazilian Midwest was conducted. Through spoligotyping and 24-loci MIRU-VNTR methods, 37 clinical isolates of M. bovis circulating in the region were analyzed, 10 isolated from the state of Mato Grosso, 12 from the state of Mato Grosso do Sul and 15 from the state of Goiás. The spoligotyping analysis identified 10 distinct M. bovis profiles (SB0121 n = 14, SB0295 n = 6, SB0140 n = 6, SB0881 n = 3, SB1144 n = 2, SB1145 n = 2, SB0134 n = 1, SB1050 n = 1, SB1055 n = 1, SB1136 n = 1) grouped in six clusters and four orphan patterns. The MIRU-VNTR 24-loci grouped the same isolates in six clusters and 22 unique orphan patterns, showing higher discriminatory power than spoligotyping. When associating the results of both techniques, the isolates were grouped in five clusters and 24 unique M. bovis profiles. Among the 24-loci MIRU-VNTR evaluated, two, ETR-A and QUB 11b loci, showed high discriminatory ability (h = ≥ 0.50), while MIRU 16, MIRU 27, ETR-B, ETR-C, Mtub21 and QUB 26 loci showed moderate ability (h = 0.33 or h = 0.49) and were the most effective in evaluating the genotypic similarities among the clinical M. bovis isolate samples. Herein, the 29 patterns found amongst the 37 isolates of M. bovis circulating in the Brazilian Midwest can be due to the animal movement between regions, municipalities and farms, thus causing the spread of various M. bovis strains in herds from Midwest Brazil.
authors.affiliation: Instituto de Química, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro/RJ, Brasil.|Faculdade de Nutrição, Universidade Federal de Mato Grosso (UFMT), Cuiabá/MT, Brasil.
authors.forename: Ricardo César Tavares
authors.initials: RC
authors.lastname: Carvalho
authors.affiliation: Laboratório de Biologia Molecular Aplicado a Micobactérias, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro/RJ, Brasil.
authors.forename: Sidra Ezidio Gonçalves
authors.initials: SE
authors.lastname: Vasconcellos
authors.affiliation: Laboratório Nacional Agropecuário (LANAGRO), Ministério da Agricultura, Pecuária e Abastecimento (MAPA), Pedro Leopoldo/MG, Brasil.
authors.forename: Marina de Azevedo
authors.initials: Mde A
authors.lastname: Issa
authors.affiliation: Laboratório Nacional Agropecuário (LANAGRO), Ministério da Agricultura, Pecuária e Abastecimento (MAPA), Pedro Leopoldo/MG, Brasil.
authors.forename: Paulo Martins
authors.initials: PM
authors.lastname: Soares Filho
authors.affiliation: Laboratório Nacional Agropecuário (LANAGRO), Ministério da Agricultura, Pecuária e Abastecimento (MAPA), Pedro Leopoldo/MG, Brasil.
authors.forename: Pedro Moacyr Pinto Coelho
authors.initials: PM
authors.lastname: Mota
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA Gado de Corte), Campo Grande/MS, Brasil.
authors.forename: Flábio Ribeiro de
authors.initials: FR
authors.lastname: Araújo
authors.affiliation: Instituto de Química, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro/RJ, Brasil.|Universidade Federal do Rio de Janeiro (UFRJ)-Campus Macaé, Macaé/RJ, Brasil.
authors.forename: Ana Carolina da Silva
authors.initials: AC
authors.lastname: Carvalho
authors.affiliation: Laboratório de Biologia Molecular Aplicado a Micobactérias, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro/RJ, Brasil.
authors.forename: Harrison Magdinier
authors.initials: HM
authors.lastname: Gomes
authors.affiliation: Laboratório de Biologia Molecular Aplicado a Micobactérias, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro/RJ, Brasil.|Mycobacteriology Unit, Tropical Institute of Medicine, Antwerp, Belgium.
authors.forename: Philip Noel
authors.initials: PN
authors.lastname: Suffys
authors.affiliation: Faculdade de Nutrição, Universidade Federal de Mato Grosso (UFMT), Cuiabá/MT, Brasil.
authors.forename: Eduardo Eustáquio de Souza
authors.identifier: http://orcid.org/0000-0002-2608-4324
authors.initials: EE
authors.lastname: Figueiredo
authors.affiliation: Instituto de Química, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro/RJ, Brasil.
authors.forename: Vânia Margaret Flosi
authors.initials: VM
authors.lastname: Paschoalin
mesh_terms: D000818:Animals; D002417:Cattle; D016000:Cluster Analysis; D005798:Genes, Bacterial; D009163:Mycobacterium bovis / Q000235:genetics*