title: Candida tropicalis biofilm inhibition by ZnO nanoparticles and EDTA.
abstract: OBJECTIVE
  Biofilm of Candida tropicalis denote as a complex cellular congregation with major implication in pathogenesis. This lifestyle of fungus as a biofilm can inhibit immune system and antifungal therapy in treatment of infectious disease especially medical device associated chronic disease. In this study effects of Zinc Oxide (ZnO) nanoparticles and EDTA were evaluated on C. tropicalis biofilm by using different techniques. ZnO nanoparticles were synthesized from Egg albumin.
  
  
  METHODS
  To assay the formation of biofilm of yeast cells like Fluconazole-susceptible C. tropicalis (ATCC 13,803) and fluconazole-resistant standard strains of C. tropicalis (ATCC 750) were grown in 24 well plates and antifungal effect of ZnO and EDTA were evaluated on C. tropicalis biofilm using ATP bioluminescence and tetrasodium salt (XTT) reduction assays.
  
  
  RESULTS
  Synthesized ZnO NPs and EDTA had effective antifungal properties at the concentration of 5.2, 8.6μg/ml for Fluconazole susceptible strain and 5.42, 10.8μg/ml Fluconazole resistant strains of C. tropicalis biofilms compared to fluconazole drug.
  
  
  CONCLUSIONS
  In present study we conclude, ZnO considered as a new agent in field of prevention C. tropicalis biofilms especially biofilms formed surface of medical device.
authors.affiliation: CAS in Marine Biology, Annamalai University, Parangipettai-608 502, Tamil Nadu, India. Electronic address: vinodbio89@gmail.com.
authors.forename: Vinoth
authors.initials: V
authors.lastname: Jothiprakasam
authors.affiliation: CAS in Marine Biology, Annamalai University, Parangipettai-608 502, Tamil Nadu, India.
authors.forename: Murugan
authors.initials: M
authors.lastname: Sambantham
authors.affiliation: CAS in Marine Biology, Annamalai University, Parangipettai-608 502, Tamil Nadu, India.
authors.forename: Stalin
authors.initials: S
authors.lastname: Chinnathambi
authors.affiliation: CAS in Marine Biology, Annamalai University, Parangipettai-608 502, Tamil Nadu, India.
authors.forename: Singaravel
authors.initials: S
authors.lastname: Vijayaboopathi
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D041022:Candida tropicalis / Q000187:drug effects*; D025141:Drug Resistance, Fungal; D004492:Edetic Acid / Q000494:pharmacology*; D015725:Fluconazole / Q000494:pharmacology; D066298:In Vitro Techniques; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D015034:Zinc Oxide / Q000138:chemical synthesis / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D015725:Fluconazole; D004492:Edetic Acid; D015034:Zinc Oxide
keywords: Antifungal agent; Biofilm; Candida tropicalis; Nanoparticles; ZnO