title: High-Throughput Sequencing-Based Immune Repertoire Study during Infectious Disease.
abstract: The selectivity of the adaptive immune response is based on the enormous diversity of T and B cell antigen-specific receptors. The immune repertoire, the collection of T and B cells with functional diversity in the circulatory system at any given time, is dynamic and reflects the essence of immune selectivity. In this article, we review the recent advances in immune repertoire study of infectious diseases, which were achieved by traditional techniques and high-throughput sequencing (HTS) techniques. HTS techniques enable the determination of complementary regions of lymphocyte receptors with unprecedented efficiency and scale. This progress in methodology enhances the understanding of immunologic changes during pathogen challenge and also provides a basis for further development of novel diagnostic markers, immunotherapies, and vaccines.
authors.affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: Dongni
authors.initials: D
authors.lastname: Hou
authors.affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Medicine, Division of Pulmonary and Critical Care Medicine, University of California San Francisco , San Francisco, CA , USA.
authors.forename: Eric John
authors.initials: EJ
authors.lastname: Seely
authors.affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: Shujing
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Song
keywords: bioinformatics; high-throughput sequencing; immune repertoire; infection; lymphocyte