title: Synthesis, characterization and anticancer activity of tanshinone I grafted low molecular chitosan.
abstract: In this study, we synthesized a novel water-soluble low molecular chitosan (LMC) derivative through Vilsmeier reaction and reductive amination reaction. The derivative was characterized by UV-visible spectroscopy,1H NMR, FTIR and SEM techniques. The results showed that the derivative effectively reduced the cell viability rate, inhibited cell metastasis, induced cell apoptosis and dissipated mitochondrial membrane potential (ΔΨm). Moreover, the antitumor activity was strengthened with the increase of the degree of substitution of tanshinone I (TanI). These findings provided important support for developing new water-soluble antitumor agent and expand the scope of application of LMC.
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.lastname: DanXu
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. ma.lili@zs-hospital.sh.cn.
authors.forename: Lili
authors.initials: L
authors.lastname: Ma
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling, Shaanxi, 712100, China. jduan@nwsuaf.edu.cn.
authors.forename: Jinyou
authors.initials: J
authors.lastname: Duan
mesh_terms: D045784:Abietanes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D016716:PC12 Cells; D051381:Rats
chemical_list: D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; C021751:tanshinone; D048271:Chitosan
keywords: Anticancer; Apoptosis; Low molecular chitosan; Metastasis; Tanshinone I