title: Neuroprotective effect of ischemic preconditioning via modulating the expression of cerebral miRNAs against transient cerebral ischemia in diabetic rats.
abstract: OBJECTIVE
  In this study, we aimed to evaluate the effect of the Ischemic preconditioning (IPreC) on the expression profile of cerebral miRNAs against stroke by induced transient middle cerebral artery occlusion (MCAo) in diabetic rats.
  
  
  METHODS
  Eighty male Spraque Dawley rats were allocated to eight groups. In order to evaluate the expression profile of miRNAs, we induced transient MCAo seven days after STZ-induced diabetes (DM). Also we performed IPreC 72 h before transient MCAo to assess whether IPreC could have a neuroprotective effect against ischemia-reperfusion injury.
  
  
  RESULTS
  The general characteristics of STZ-treated rats included reduced body weight and elevated blood glucose levels compared to non-diabetic ones. We demonstrated that miRNA expression profiles, which are determined for biological functions such as aquaporin 4 formation (miR-29b-2, miR-124a-3p, miR-130a, miR-223 and miR-320a), glutamate toxicity (miR107, miR-145, miR-223), salvageable ischemic area (miR-9a, miR-19b, miR-29b-2, miR-341, miR-339-5p, miR-15-5p, miR-99b-5p), and neoangiogenesis (let-7f-5p, miR-126a and miR-322-3p), were regulated following IPreC. Ischemic preconditioning before cerebral ischemia significantly reduced infarction size compared with the other groups [IPreC + MCAo (27 ± 11 mm3) vs. MCAo (109 ± 15 mm3) p < 0.001; DM + IPreC + MCAo (38 ± 9 mm3) vs. DM + MCAo (165 ± 41 mm3) p < 0.001, respectively].
  
  
  CONCLUSIONS
  The study results revealed the neuroprotective effects of ischemic preconditioning, supported with the upregulated pro-survival miRNAs in MCA infarcts.
authors.affiliation: a Neurology Clinic , Bor State Hospital , Nigde , Turkey.
authors.forename: Ozge
authors.initials: O
authors.lastname: Altintas
authors.affiliation: b Faculty of Engineering, Department of Genetics and Bioengineering , Fatih University , Istanbul , Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozgen Altintas
authors.affiliation: c Vocational School of Health Services, Medical Laboratory Techniques , BezmiAlem Vakif University , Istanbul , Turkey.
authors.forename: Meltem
authors.initials: M
authors.lastname: Kumas
authors.affiliation: d Medical Faculty, Department of Neurology , BezmiAlem Vakıf University , Istanbul , Turkey.
authors.forename: Talip
authors.initials: T
authors.lastname: Asil
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D020520:Brain Infarction / Q000473:pathology / Q000517:prevention & control; D002545:Brain Ischemia / Q000150:complications*; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D004195:Disease Models, Animal; D007839:Functional Laterality; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009422:Nervous System Diseases / Q000209:etiology* / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric; D013311:Streptozocin / Q000494:pharmacology; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D035683:MicroRNAs; D013311:Streptozocin
keywords: Neuronal survival; Neuroprotection; Preconditioning; Transient Ischemia; miRNA