title: Comparative Gut Microbiomes of Four Species Representing the Higher and the Lower Termites.
abstract: Aiming at learning the association between the gut microbiota and termites with different diet habits and phylogenetic positions, the gut bacteria of three populations for each of the two higher termites (wood-feeding Mironasutitermes shangchengensis and fungus-feeding Odontotermes formosanus) and two wood-feeding lower termites (Tsaitermes ampliceps and Reticulitermes flaviceps) were analyzed by high-throughput 454 pyrosequencing of 16S V1-V3 amplicons. As results, 132 bacterial genera and some unidentified operational taxonomic units within 29 phyla in the gut bacteria were detected, with Spirochaetes (11-55%), Firmicutes (7-18%), Bacteroidetes (7-31%), and Proteobacteria (8-14%) as the main phyla, and Treponema, TG5, Dysgonomonas, Tannerella, za29, Lactococcus, Pseudomonas, and SJA-88 as the common genera in all the four termites. The diversity of gut bacterial communities in the higher termite guts was significantly greater than that in the lower termites; while the gut microbiota in M. shangchengensis (wood-feeding higher termite) was more similar to those of the wood-feeding lower termites rather than that of O. formosanus (fungus-feeding higher termite), and phylum Spirochaetes and nitrogen-fixing bacteria were super-dominant in the wood-feeding termites, despite of their phylogenetic relations. This study reported for the first time the gut bacterial communities for the termites of M. shangchengensis and T. ampliceps and the comparative analyses showed that the gut microbial communities varied according to the phylogeny and the diet habits of termites.
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: LiJuan
authors.initials: L
authors.lastname: Su
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: LeLe
authors.initials: L
authors.lastname: Yang
authors.affiliation: BioEnergy Genome Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (xujian@qibebt.ac.cn; songandong@henau.edu.cn).
authors.forename: Shi
authors.initials: S
authors.lastname: Huang
authors.affiliation: BioEnergy Genome Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (xujian@qibebt.ac.cn; songandong@henau.edu.cn).
authors.forename: XiaoQuan
authors.initials: X
authors.lastname: Su
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan 450002, China Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, Zhengzhou, Henan 450002, China.
authors.forename: FengQin
authors.initials: F
authors.lastname: Wang
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, DF 11340, México.
authors.forename: EnTao
authors.initials: E
authors.lastname: Wang
authors.affiliation: BioEnergy Genome Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (xujian@qibebt.ac.cn; songandong@henau.edu.cn).
authors.forename: Ning
authors.initials: N
authors.lastname: Kang
authors.affiliation: BioEnergy Genome Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (xujian@qibebt.ac.cn; songandong@henau.edu.cn).
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan 450002, China Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, Zhengzhou, Henan 450002, China songandong@henau.edu.cn.
authors.forename: AnDong
authors.initials: A
authors.lastname: Song
mesh_terms: D000818:Animals; D001419:Bacteria / Q000166:cytology / Q000235:genetics*; D000069196:Gastrointestinal Microbiome / Q000235:genetics / Q000502:physiology*; D020049:Isoptera / Q000382:microbiology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
keywords: bacterial diversity; comparative study; gut microbiota; pyrosequencing; termite