title: Levels of DNA damage in peripheral blood lymphocytes of patients undergoing standard hemodialysis vs on-line hemodiafiltration: A comet assay investigation.
abstract: Chronic kidney disease (CKD) patients exhibit high levels of genetic damage. Part of this genetic damage is supposed to be caused by the hemodialysis (HD) therapy. Different and more efficient HD procedures could reduce the genetic damage and improve health status of CKD patients. In the present study, we analyzed if changing to online hemodiafiltration (OL-HDF) has a beneficial effect on the levels of genetic damage. The levels of genetic damage (DNA breaks and oxidatively damaged DNA) were analyzed in peripheral blood lymphocytes by using the comet assay. Forty-nine patients submitted to HD, 34 of them changing to OL-HDF and 15 patients continuing in low-flux HD, were included in the study. Plasma antioxidant capacity was also determined. Second sampling period was established after 6 months on the new or traditional HD protocol. A slight decrease in the levels of DNA damage was observed in patients who switched to OL-HDF (P=0.048) in relation to the reference group. This reduction is indicative that OL-HDF shows greater efficiency than low-flux HD in the reduction of basal levels of genetic damage.
authors.affiliation: Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici C, Universitat Autònoma de Barcelona, 08193 Bellaterra, Cerdanyola del Vallès, Spain.
authors.forename: Zuray
authors.initials: Z
authors.lastname: Corredor
authors.affiliation: Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici C, Universitat Autònoma de Barcelona, 08193 Bellaterra, Cerdanyola del Vallès, Spain.
authors.forename: Lara
authors.initials: L
authors.lastname: Rodríguez-Ribera
authors.affiliation: Fundació Puigvert, Barcelona, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Silva
authors.affiliation: Fundació Puigvert, Barcelona, Spain.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Díaz
authors.affiliation: Fundació Puigvert, Barcelona, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Ballarín
authors.affiliation: Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici C, Universitat Autònoma de Barcelona, 08193 Bellaterra, Cerdanyola del Vallès, Spain; CIBER Epidemiología y Salud Pública, ISCIII, Madrid, Spain.
authors.forename: Ricard
authors.initials: R
authors.lastname: Marcos
authors.affiliation: Fundació Puigvert, Barcelona, Spain.
authors.forename: Elisabet
authors.initials: E
authors.lastname: Coll
authors.affiliation: Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici C, Universitat Autònoma de Barcelona, 08193 Bellaterra, Cerdanyola del Vallès, Spain; CIBER Epidemiología y Salud Pública, ISCIII, Madrid, Spain. Electronic address: susana.pastor@uab.es.
authors.forename: Susana
authors.initials: S
authors.lastname: Pastor
mesh_terms: D001774:Blood Chemical Analysis; D002318:Cardiovascular Diseases / Q000209:etiology / Q000235:genetics; D020552:Comet Assay; D004249:DNA Damage; D003920:Diabetes Mellitus / Q000209:etiology / Q000235:genetics; D050171:Dyslipidemias / Q000209:etiology / Q000235:genetics; D005260:Female; D017583:Hemodiafiltration / Q000009:adverse effects / Q000379:methods; D006801:Humans; D006973:Hypertension / Q000209:etiology / Q000235:genetics; D008214:Lymphocytes / Q000473:pathology / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis / Q000009:adverse effects / Q000379:methods*; D051436:Renal Insufficiency, Chronic / Q000150:complications / Q000235:genetics* / Q000628:therapy*
keywords: Chronic kidney disease; Comet assay; On-line hemodiafiltration; Peripheral blood lymphocytes