title: Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo.
abstract: The leaves of Artemisia argyi Lev. et Vant. and A. princeps Pamp. are well known medicinal herbs used to treat patients in China, Japan, and Korea with skin problems such as eczema and itching, as well as abdominal pain and dysmenorrhoea. We investigated the anti-inflammatory effects of Artemisia leaf extract (ALE) using CD mice and Raw 264.7 cells. The effects of ALE on histopathological changes and cytokine production in ear tissues were assessed in mice with CD induced by 1-fluoro-2,4-dinitrobenzene (DNFB). Moreover, the anti-inflammatory effects on production levels of prostaglandin E2 (PGE2) and nitric oxide (NO) and expression levels of cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) were investigated in Raw 264.7 cells. Topical application of ALE effectively prevented ear swelling induced by repeated DNFB application. ALE prevented epidermal hyperplasia and infiltration of immune cells and lowered the production of interferon- (IFN-) gamma (γ), tumour necrosis factor- (TNF-) alpha (α), and interleukin- (IL-) 6 in inflamed tissues. In addition, ALE inhibited expression of COX-2 and iNOS and production of NO and PGE2 in Raw 264.7 cells. These results indicate that Artemisia leaf can be used as a therapeutic agent for inflammatory skin diseases and that its anti-inflammatory effects are closely related to the inhibition of inflammatory mediator release from macrophages and inflammatory cytokine production in inflamed tissues.
authors.affiliation: Division of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of Korea.
authors.forename: Chanyong
authors.initials: C
authors.lastname: Yun
authors.affiliation: Division of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of Korea.
authors.forename: Youngchul
authors.initials: Y
authors.lastname: Jung
authors.affiliation: Division of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of Korea.
authors.forename: Wonjoo
authors.initials: W
authors.lastname: Chun
authors.affiliation: Division of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of Korea.
authors.forename: Beodeul
authors.initials: B
authors.lastname: Yang
authors.affiliation: Division of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of Korea.
authors.forename: Junghyun
authors.initials: J
authors.lastname: Ryu
authors.affiliation: College of Medicine, Dongguk University, Goyang-si, Gyeonggi-do 10326, Republic of Korea.
authors.forename: Chiyeon
authors.identifier: 0000-0003-0178-6976
authors.initials: C
authors.lastname: Lim
authors.affiliation: Division of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of Korea.
authors.forename: Jung-Hoon
authors.identifier: 0000-0002-6468-6510
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Division of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of Korea.
authors.forename: Hyungwoo
authors.identifier: 0000-0002-1183-7434
authors.initials: H
authors.lastname: Kim
authors.affiliation: Division of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of Korea.
authors.forename: Su-In
authors.initials: SI
authors.lastname: Cho
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry*; D002681:China; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003877:Dermatitis, Contact / Q000188:drug therapy*; D004139:Dinitrofluorobenzene / Q000737:chemistry; D015232:Dinoprostone / Q000378:metabolism; D004817:Epidermis / Q000473:pathology; D006965:Hyperplasia / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy; D007371:Interferon-gamma / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D000067996:RAW 264.7 Cells; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D015850:Interleukin-6; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D004139:Dinitrofluorobenzene; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D015232:Dinoprostone