title: The Role of Lysophospholipid Acyltransferases in the Golgi Complex.
abstract: Determining the abundance of phospholipids and neutral lipids in cellular membranes is paramount to understanding their biological functions. Many lipid-modifying enzymes have yet to be characterized due to limitations in substrate-product measurements and purification of membrane-bound enzymes. The method described here uses radiolabeled phospholipid substrates and cell-purified organelles to quantify phospholipid metabolism using thin-layer chromatography. This assay has the benefits of being specific and adaptable for numerous applications and systems.
authors.affiliation: Department of Biology, Villanova University, Mendel Hall Rm G63B, 800 Lancaster Avenue, Villanova, PA, 19085, USA. john.a.schmidt@villanova.edu.
authors.forename: John A
authors.initials: JA
authors.lastname: Schmidt
mesh_terms: D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism*; D000818:Animals; D006056:Golgi Apparatus / Q000201:enzymology*; D006801:Humans
chemical_list: D008243:1-Acylglycerophosphocholine O-Acyltransferase
keywords: Acyltransferase; Golgi membranes; LPAAT; LPAT; Lysophosphatidic acid; Phosphatidic acid