title: Identification of more objective biomarkers for Blood-Stasis syndrome diagnosis.
abstract: BACKGROUND
  Blood-stasis syndrome (BSS) is one of the Traditional Chinese medicine (TCM) syndrome differentiations that are commonly seen in stroke and ischemic heart diseases; however, the BSS differentiation criterion is not standardized. More objective biomarkers for BSS diagnosis are needed.
  
  
  METHODS
  Acute ischemic stroke (AIS) or unstable angina (UA) patients with BSS and healthy controls were enrolled. The miRNA and mRNA expression profiles of UA patients and AIS patients were compared to those of healthy controls to identify the differentially expressed miRNA and mRNA of BSS. Bioinformatics analysis was used to identify significantly deregulated miRNAs and mRNAs correlated to BSS. QRT-PCR was performed to validate the bioinformatics analysis results.
  
  
  RESULTS
  Approximately 401 mRNAs and 11 miRNAs were differentially expressed in both UA and AIS patients compared to healthy controls. Gene ontology (GO) functional analysis was performed, and multiple GO terms were enriched. Among the overlapping DE miRNAs and mRNAs, miR-146b-5p, -199a-5p and 23 targeted mRNAs were pivotal genes in the BSS genomic characteristics. These 2 miRNAs and 23 mRNAs formed network-type biomarkers for BSS.
  
  
  CONCLUSIONS
  The genomic characteristics of BSS were shown in this study. miR-146b-5p, -199a-5p and the 23 targeted mRNAs formed a diagnostic network for BSS. Further improvement and validation of this diagnostic network might lead to more objective diagnostic criteria for BSS.
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, No.5 Beixiange Street, Xicheng District, Beijing, 100053, China.|Graduate School, Beijing University of Chinese Medicine, No.11 Beisanhuan East Road, Chaoyang District, Beijing, 100029, China.
authors.forename: Jiangquan
authors.initials: J
authors.lastname: Liao
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, No.5 Beixiange Street, Xicheng District, Beijing, 100053, China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, No.5 Beixiange Street, Xicheng District, Beijing, 100053, China. wangjie0103@126.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
mesh_terms: D000368:Aged; D000787:Angina Pectoris / Q000097:blood / Q000175:diagnosis; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D019295:Computational Biology; D005260:Female; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional / Q000379:methods*; D008875:Middle Aged; D020521:Stroke / Q000097:blood / Q000175:diagnosis
chemical_list: D015415:Biomarkers