title: Immunization with live nonpathogenic H5N3 duck influenza virus protects chickens against highly pathogenic H5N1 virus.
abstract: Development of an effective, broadly-active and safe vaccine for protection of poultry from H5N1 highly pathogenic avian influenza viruses (HPAIVs) remains an important practical goal. In this study we used a low pathogenic wild aquatic bird virus isolate А/duck/Moscow/4182/2010 (H5N3) (dk/4182) as a live candidate vaccine. We compared this virus with four live 1:7 reassortant anti-H5N1 candidate vaccine viruses with modified hemagglutinin from either A/Vietnam/1203/04 (H5N1) or A/Kurgan/3/05 (H5N1) and the rest of the genes from either H2N2 cold-adapted master strain A/Leningrad/134/17/57 (rVN-Len and rKu-Len) or H6N2 virus A/gull/Moscow/3100/2006 (rVN-gull and rKu-gull). The viruses were tested in parallel for pathogenicity, immunogenicity and protective effectiveness in chickens using aerosol, intranasal and oral routes of immunization. All five viruses showed zero pathogenicity indexes in chickens. Viruses rVN-gull and rKu-gull were immunogenic and protective, but they were insufficiently attenuated and caused significant mortality of 1-day-old chickens. The viruses with cold-adapted backbones (rVN-Len and rKu-Len) were completely nonpathogenic, but they were significantly less immunogenic and provided lower protection against lethal challenge with HPAIV A/Chicken/Kurgan/3/05 (H5N1) as compared with three other vaccine candidates. Unlike other four viruses, dk/4182 was both safe and highly immunogenic in chickens of any age regardless of inoculation route. Single administration of 106 TCID50 of dk/4182 virus via drinking water provided complete protection of 30-days-old chickens from 100 LD50 of the challenge virus. Our results suggest that low pathogenic viruses of wild aquatic birds can be used as safe and effective live poultry vaccines against highly pathogenic avian viruses.
authors.forename: A S
authors.initials: AS
authors.lastname: Gambaryan
authors.forename: E Y
authors.initials: EY
authors.lastname: Boravleva
authors.forename: N F
authors.initials: NF
authors.lastname: Lomakina
authors.forename: E A
authors.initials: EA
authors.lastname: Kropotkina
authors.forename: I V
authors.initials: IV
authors.lastname: Gordeychuk
authors.forename: I A
authors.initials: IA
authors.lastname: Chvala
authors.forename: V V
authors.initials: VV
authors.lastname: Drygin
authors.forename: H-D
authors.initials: HD
authors.lastname: Klenk
authors.forename: M N
authors.initials: MN
authors.lastname: Matrosovich
mesh_terms: D000284:Administration, Oral; D000375:Aging; D000818:Animals; D002645:Chickens; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D016679:Genome, Viral; D007114:Immunization; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology* / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D005585:Influenza in Birds / Q000517:prevention & control*; D011201:Poultry Diseases / Q000517:prevention & control* / Q000821:virology; D014774:Virulence
chemical_list: D007252:Influenza Vaccines
keywords: influenza virus A; H5N1; poultry vaccine.