title: PRAME is critical for breast cancer growth and metastasis.
abstract: Breast cancer is the most common cause of cancer death in women and ranks second among cancer deaths. Metastasis is the main cause of death in breast cancer patients. However, the mechanisms underlying the invasion and metastasis of breast cancer cells remain largely elusive. Here we report that the protein PRAME, a tumor-associated antigen isolated from a melanoma, plays a role in preventing the proliferation and metastasis of breast cancer cells. Knocking down of PRAME promotes breast cancer cell proliferation and inhibits apoptosis. In addition, inhibition of PRAME promotes the invasion of breast cancer cells. To further examine the role of PRAME in vivo, we utilized mouse model and found the volume and the weight of tumors was markedly increased after PRAME was knocked down. This study demonstrates that PRAME functions as a tumor suppressor in breast cancer.
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, the Second Military Medical University, Shanghai, PR China.
authors.forename: Zhengwang
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, the Second Military Medical University, Shanghai, PR China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, the 411th Hospital of PLA, Shanghai, PR China.
authors.forename: Fenglin
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, the Second Military Medical University, Shanghai, PR China; Department of Orthopedic Oncology, Changzheng Hospital, the Second Military Medical University, Shanghai, PR China.
authors.forename: Qunfeng
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, the Second Military Medical University, Shanghai, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.affiliation: Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Shanghai Bone Tumor Institute, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, PR China. Electronic address: Dianwen_Song@163.com.
authors.forename: Dianwen
authors.initials: D
authors.lastname: Song
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, the Second Military Medical University, Shanghai, PR China. Electronic address: Quan_Huang1234@163.com.
authors.forename: Quan
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai 200241, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, the Second Military Medical University, Shanghai, PR China. Electronic address: jianru_xiao12345@163.com.
authors.forename: Jianru
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000951:Antigens, Neoplasm; C404944:PRAME protein, human; D025521:Tumor Suppressor Proteins
keywords: Breast cancer; E-cadherin; PRAME