title: Glucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere-attached l-glycine.
abstract: In the present study, a novel biosensor that is sensitive to glucose was prepared using the microspheres modified with (4-formyl-3-methoxyphenoxymethyl)polystyrene (FMPS) with l-glycine. Polymeric microspheres having Schiff bases were prepared from FMPS using the glycine condensation method. Glucose oxidase enzyme was immobilized onto modified carbon paste electrode by cross-linking with glutaraldehyde. Oxidation of enzymatically produced H2O2(+0.5 V vs. Ag/AgCl) was used for determination of glucose. Optimal temperature and pH were found as 50 °C and 8.0, respectively. The glucose biosensor showed a linear working range from 5.0 × 10-4to 1.0 × 10-2M, R2= 0.999. Storage and operational stability of the biosensor were also investigated. The biosensor gave perfect reproducible results after 20 measurements with 3.3% relative standard deviation. It also had good storage stability.
authors.affiliation: School of Health, Sinop University, Sinop, Turkey.
authors.forename: Soner
authors.initials: S
authors.lastname: Donmez
authors.affiliation: Department of Chemistry, Faculty of Science, Gazi University, Ankara, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Arslan
authors.affiliation: Department of Chemistry, Faculty of Science, Gazi University, Ankara, Turkey.
authors.forename: Nurşen
authors.initials: N
authors.lastname: Sarı
authors.affiliation: Department of Chemistry, Faculty of Science, Gazi University, Ankara, Turkey.
authors.forename: Elvan
authors.initials: E
authors.lastname: Hasanoğlu Özkan
authors.affiliation: Department of Chemistry, Faculty of Science, Gazi University, Ankara, Turkey.
authors.forename: Halit
authors.initials: H
authors.lastname: Arslan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D002244:Carbon / Q000737:chemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D005998:Glycine / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D008863:Microspheres; D008954:Models, Biological; D011137:Polystyrenes; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D011137:Polystyrenes; D002244:Carbon; D005949:Glucose Oxidase; D005998:Glycine
keywords: Schiff base; biosensor; carbon paste; glucose oxidase; microsphere; polystyrene