title: Unexpectedly High Beta-Diversity of Root-Associated Fungal Communities in the Bolivian Andes.
abstract: Bolivia is one of the most biologically diverse countries on the planet. Between the Andes and the Amazon drainage basin spans the Yungas, a vast forested region shown to be extremely species rich in macro-organisms. However, it remains unclear whether this high diversity is also reflected in microbial diversity. Here we assess the genetic, taxonomic and functional diversity of root-associated fungi surrounding Cinchona calisaya trees, a typical element of the intermediate altitudes of the Bolivian Yungas. We determine the relative effects of edaphic properties, climate, and geography in regulating fungal community assembly. We show that α-diversity for these fungal communities was similar to temperate and arid ecosystems, averaging 90.1 operational taxonomic units (OTUs) per sample, with reads predominantly assigned to the Ascomycota phylum and with a saprotrophic lifestyle. ß-diversity was calculated as the distance-decay rate, and in contrast to α-diversity, was exceptionally high with a rate of -0.407. Soil properties (pH and P) principally regulated fungal community assembly in an analogous manner to temperate environments, with pH and phosphorus explaining 7.8 and 7.2% of community variation respectively. Surprisingly, altitude does not influence community formation, and there is limited evidence that climate (precipitation and temperature) play a role. Our results suggest that sampling should be performed over a wide geographical and environmental range in order to capture the full root-associated fungal diversity in subtropical regions. This study sheds further light on the diversity and distribution of the world's "hidden biodiversity."
authors.affiliation: Nina Rønsted Lab, Natural History Museum of Denmark, University of Copenhagen Copenhagen, Denmark.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Barnes
authors.affiliation: Nina Rønsted Lab, Natural History Museum of Denmark, University of CopenhagenCopenhagen, Denmark; Herbario Nacional de Bolivia, Universidad Mayor de San AndresLa Paz, Bolivia.
authors.forename: Carla
authors.initials: C
authors.lastname: Maldonado
authors.affiliation: Department of Biological and Environmental Sciences, University of GothenburgGothenburg, Sweden; Gothenburg Botanical GardenGothenburg, Sweden.
authors.forename: Tobias G
authors.initials: TG
authors.lastname: Frøslev
authors.affiliation: Department of Biological and Environmental Sciences, University of GothenburgGothenburg, Sweden; Gothenburg Botanical GardenGothenburg, Sweden.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Antonelli
authors.affiliation: Nina Rønsted Lab, Natural History Museum of Denmark, University of Copenhagen Copenhagen, Denmark.
authors.forename: Nina
authors.initials: N
authors.lastname: Rønsted
keywords: Cinchona calisaya; beta-diversity; fungal spatial-scaling; root-associated fungi; soil fungi