title: RNase attenuates acute lung injury induced by ischemia-reperfusion in mice.
abstract: Treatment with ribonuclease (RNase) reportedly protects the heart after myocardial ischemia-reperfusion (I/R), but its potential effect on lung I/R injury (LIRI) is unknown. Thus, we aim to explore whether RNase treatment would relieve LIRI. Thirty-six C57BL/6J adult male wild-type mice were evenly divided into I/R+RNase (I/R+R) group, I/R group, and sham group. Lung I/R was induced by left pulmonary hilum occlusion for 1h and reperfusion for 2h. All mice were treated with RNase or same dosage of normal saline in advance. After I/R, blood and lung tissues were collected for analysis. The results showed that lung injury scores, wet/dry ratio, expressions of inflammatory cytokines, pulmonary apoptosis, and levels of serum extracellular RNA (exRNA), including microRNAs, were markedly elevated after I/R. However, RNase treatment significantly attenuated cytokine production in both lung tissue and serum and also suppressed pulmonary apoptosis as reflected by TUNEL staining and activated caspase-3. In addition, total serum exRNA levels in the I/R+R group had a downward trend versus the I/R group. In conclusion, the increase of circulating exRNA levels contributed to LIRI in adult mice, which could be relieved by injection of RNase during perioperative window. The potential mechanism is the decrease of serum exRNA level and the suppression of pulmonary inflammation and apoptosis.
authors.affiliation: Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, China. Electronic address: zhangxiy07@163.com.
authors.forename: Xi-Yang
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, China. Electronic address: xychenchan@gmail.com.
authors.forename: Chan
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Southwest Medical University, Luzhou, China. Electronic address: baiyiping0608@163.com.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Bai
authors.affiliation: Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ma
authors.affiliation: Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, China.
authors.forename: Ya-Bing
authors.initials: YB
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, China. Electronic address: liubinhxyy@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
mesh_terms: D055371:Acute Lung Injury / Q000097:blood / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D000818:Animals; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000097:blood / Q000235:genetics; D012313:RNA / Q000097:blood*; D015427:Reperfusion Injury / Q000097:blood / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D012260:Ribonucleases / Q000494:pharmacology / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics
chemical_list: D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; D012313:RNA; D012260:Ribonucleases
keywords: RNase; apoptosis; exRNA; inflammation; ischemia; reperfusion