title: A deep sequencing analysis of transcriptomes and the development of EST-SSR markers in mungbean (Vigna radiata).
abstract: Mungbean (Vigna radiata L. Wilczek) is one of the most important leguminous food crops in Asia. We employed Illumina paired-end sequencing to analyse transcriptomes of three different mungbean genotypes. A total of 38.3-39.8 million pairedend reads with 73 bp lengths were generated. The pooled reads from the three libraries were assembled into 56,471 transcripts. Following a cluster analysis, 43,293 unigenes were obtained with an average length of 739 bp and N50 length of 1176 bp. Of the unigenes, 34,903 (80.6%) had significant similarity to known proteins in the NCBI nonredundant protein database (Nr), while 21,450 (58.4%) had BLAST hits in the Swiss-Prot database (E-value<10⁻⁵). Further, 1245 differential expression genes were detected among three mungbean genotypes. In addition, we identified 3788 expressed sequence tag-simple sequence repeat (EST-SSR) motifs that could be used as potential molecular markers. Among 320 tested loci, 310 (96.5%) yielded amplification products, and 151 (47.0%) exhibited polymorphisms among six mungbean accessions. These transcriptome data and mungbean EST-SSRs could serve as a valuable resource for novel gene discovery and the marker-assisted selective breeding of this species.
authors.affiliation: Key Laboratory of Crop Genetics and Breeding of Hebei Province, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, People's Republic of China.nkytianjing@163.com.
authors.forename: Changyou
authors.initials: C
authors.lastname: Liu
authors.forename: Baojie
authors.initials: B
authors.lastname: Fan
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Cao
authors.forename: Qiuzhu
authors.initials: Q
authors.lastname: Su
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhixiao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
mesh_terms: D000595:Amino Acid Sequence; D030562:Databases, Protein; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D018895:Microsatellite Repeats; D058977:Molecular Sequence Annotation; D059372:Nucleotide Motifs; D000069600:Plant Breeding; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D059467:Transcriptome; D000070658:Vigna / Q000235:genetics*
chemical_list: D005819:Genetic Markers