title: Construction of stabilized and tagged foot-and-mouth disease virus.
abstract: Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease that affects cloven-hoofed animals worldwide. Construction and purification of stable antigen for vaccine are necessary but technically difficult and laborious. Here, we have tried to investigate an alternative method by inserting a hexa-histidine tag (6xHIS) in the VP1 C-terminal for easy purification and replacing two amino acids of VP1/VP2 to enhance the stability of the capsid of the FMD virus (FMDV) Asia1/MOG/05. In addition, infectious 6xHIS-tagged stable (S/T) FMDVs were maintained under acidic conditions (pH 6.0) and were readily purified from small-scale cultures using a commercial metal-affinity column. The groups vaccinated with the S/T FMDV antigen showed complete protection comparing to low survival rate in the group vaccinated with non-S/T FMDV against lethal challenge with Asia1 Shamir in mice. Therefore, the present findings indicate that the stabilized and tagged antigen offers an alternative to using the current methods for antigen purification and enhancement of stability and has potential for the development of a new FMD vaccine.
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Jeong-Nam
authors.initials: JN
authors.lastname: Park
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Mi-Kyeong
authors.initials: MK
authors.lastname: Ko
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Rae-Hyung
authors.initials: RH
authors.lastname: Kim
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Min-Eun
authors.initials: ME
authors.lastname: Park
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Seo-Yong
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Yoon
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Joo-Hyung
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Su-Hwa
authors.initials: SH
authors.lastname: You
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Jung-Won
authors.initials: JW
authors.lastname: Park
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Kwang-Nyeong
authors.initials: KN
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Chun
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Su-Mi
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Dongseob
authors.initials: D
authors.lastname: Tark
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Hyang-Sim
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Ko
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Byounghan
authors.initials: B
authors.lastname: Kim
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea.
authors.forename: Myoung-Heon
authors.initials: MH
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do, 39660, Republic of Korea. Electronic address: parkjhvet@korea.kr.
authors.forename: Jong-Hyeon
authors.initials: JH
authors.lastname: Park
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology* / Q000302:isolation & purification; D036022:Capsid Proteins / Q000235:genetics; D005536:Foot-and-Mouth Disease / Q000517:prevention & control / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000276:immunology*; D006639:Histidine / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D055550:Protein Stability; D064166:Vaccine Potency; D014614:Vaccines, Synthetic; D014765:Viral Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; C442459:VP2 protein, Foot-and-mouth disease virus; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D006639:Histidine
keywords: Foot-and-mouth disease vaccine; Stability; Tag