title: Mechanistic insights into class B radical-S-adenosylmethionine methylases: ubiquitous tailoring enzymes in natural product biosynthesis.
abstract: Class B radical S-adenosylmethionine (SAM) methylases are notable for their ability to catalyse methylation reactions in the biosynthesis of a wide variety of natural products, including polyketides, ribosomally biosynthesised and post-translationally modified peptides (RiPPs), nonribosomal peptides (NRPs), aminoglycosides, β-lactams, phosphonates, enediynes, aminocoumarins and terpenes. Here, we discuss the diversity of substrates and catalytic mechanism utilised by such enzymes, highlighting the stereochemical course of methylation reactions at un-activated carbon centres and the ability of some members of the family to catalyse multiple methylations.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: Lona M
authors.initials: LM
authors.lastname: Alkhalaf
authors.affiliation: Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: Emmanuel Lc
authors.initials: EL
authors.lastname: de Los Santos
authors.affiliation: Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK. Electronic address: G.L.Challis@warwick.ac.uk.
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Challis
mesh_terms: D001688:Biological Products / Q000378:metabolism*; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism*
chemical_list: D001688:Biological Products; D012436:S-Adenosylmethionine; D008780:Methyltransferases