title: Antibiotic resistance patterns and genetic relatedness ofEnterococcus faecalisandEnterococcus faeciumisolated from military working dogs in Korea.
abstract: Enterococcusspp. are normally present in the gastrointestinal tracts of animals and humans, but can cause opportunistic infections that can be transmitted to other animals or humans with integrated antibiotic resistance. To investigate if this is a potential risk in military working dogs (MWDs), we analyzed antibiotic resistance patterns and genetic relatedness ofEnterococcusspp. isolated from fecal samples of MWDs of four different age groups. Isolation rates ofEnterococcusspp.,Enterococcus(E.)faecalis, andE. faecium, were 87.7% (57/65), 59.6% (34/57), and 56.1% (32/57), respectively, as determined by bacterial culture and multiplex PCR. The isolation rate ofE. faecalisgradually decreased with age (puppy, 100%; adolescent, 91.7%; adult, 36.4%; and senior, 14.3%). Rates of resistance to the antibiotics ciprofloxacin, gentamicin, streptomycin, sulfamethoxazole/trimethoprim, imipenem, and kanamycin amongEnterococcusspp. increased in adolescents and adults and decreased in senior dogs, with some isolates having three different antibiotic resistance patterns. There were indistinguishable pulsed-field gel electrophoresis patterns among the age groups. The results suggest thatEnterococcusis horizontally transferred, regardless of age. As such, periodic surveillance studies should be undertaken to monitor changes in antibiotic resistance, which may necessitate modification of antibiotic regimens to manage antibiotic resistance transmission.
authors.affiliation: BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
authors.forename: Kiman
authors.initials: K
authors.lastname: Bang
authors.affiliation: BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
authors.forename: Jae-Uk
authors.initials: JU
authors.lastname: An
authors.affiliation: BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
authors.forename: Woohyun
authors.initials: W
authors.lastname: Kim
authors.affiliation: BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Dong
authors.affiliation: BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
authors.forename: Junhyung
authors.initials: J
authors.lastname: Kim
authors.affiliation: BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
authors.forename: Seongbeom
authors.initials: S
authors.lastname: Cho
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D004283:Dog Diseases / Q000188:drug therapy / Q000382:microbiology*; D004285:Dogs; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D008297:Male; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D008889:Military Personnel; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary; D056910:Republic of Korea
chemical_list: D000900:Anti-Bacterial Agents
keywords: Enterococcus faecalis; Enterococcus faecium; antibiotic resistance; large-breed dog; pulsed-field gel electrophoresis