title: Secretory COPII Protein SEC31B Is Required for Pollen Wall Development.
abstract: The pollen wall protects pollen grains from abiotic and biotic stresses. During pollen wall development, tapetal cells play a vital role by secreting proteins, signals, and pollen wall material to ensure microspore development. But the regulatory mechanism underlying the secretory pathway of the tapetum is largely unknown. Here, we characterize the essential role of the Arabidopsis (Arabidopsis thaliana) COPII protein SECRETORY31B (SEC31B) in pollen wall development and the secretory activity of tapetal cells. The sporophyte-controlled atsec31b mutant exhibits severe pollen and seed abortion. Transmission electron microscopy observation indicates that pollen exine formation in the atsec31b mutant is disrupted significantly. AtSEC31B is a functional COPII protein revealed by endoplasmic reticulum (ER) exit site localization, interaction with AtSEC13A, and retarded ER-Golgi protein trafficking in the atsec31b mutant. A genetic tapetum-specific rescue assay indicates that AtSEC31B functions primarily in the tapetum. Moreover, deletion of AtSEC31B interrupted the formation of the ER-derived tapetosome and altered the location of the ATP-BINDING CASSETTE TRANSPORTER9 protein in the tapetum. Therefore, this work demonstrates that AtSEC31B plays a vital role in pollen wall development by regulating the secretory pathway of the tapetal cells.
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology and Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Shijia Zhuang 050024, People's Republic of China; and.|Hebei Collaboration Innovation Center for Cell Signaling, Shijia Zhuang 050024, People's Republic of China.
authors.forename: Bingchun
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology and Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Shijia Zhuang 050024, People's Republic of China; and.|Hebei Collaboration Innovation Center for Cell Signaling, Shijia Zhuang 050024, People's Republic of China.
authors.forename: Haidan
authors.initials: H
authors.lastname: Shi
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology and Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Shijia Zhuang 050024, People's Republic of China; and.|Hebei Collaboration Innovation Center for Cell Signaling, Shijia Zhuang 050024, People's Republic of China.
authors.forename: Wanlei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology and Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Shijia Zhuang 050024, People's Republic of China; and.|Hebei Collaboration Innovation Center for Cell Signaling, Shijia Zhuang 050024, People's Republic of China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology and Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Shijia Zhuang 050024, People's Republic of China; and.|Hebei Collaboration Innovation Center for Cell Signaling, Shijia Zhuang 050024, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology and Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Shijia Zhuang 050024, People's Republic of China; and.|Hebei Collaboration Innovation Center for Cell Signaling, Shijia Zhuang 050024, People's Republic of China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology and Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Shijia Zhuang 050024, People's Republic of China; and.|Hebei Collaboration Innovation Center for Cell Signaling, Shijia Zhuang 050024, People's Republic of China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology and Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Shijia Zhuang 050024, People's Republic of China; and.|Hebei Collaboration Innovation Center for Cell Signaling, Shijia Zhuang 050024, People's Republic of China.
authors.forename: Meidi
authors.initials: M
authors.lastname: Yang
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology and Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Shijia Zhuang 050024, People's Republic of China; and ruili@mail.hebtu.edu.cn guoyi@mail.hebtu.edu.cn.|Hebei Collaboration Innovation Center for Cell Signaling, Shijia Zhuang 050024, People's Republic of China ruili@mail.hebtu.edu.cn guoyi@mail.hebtu.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology and Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, College of Life Science, Hebei Normal University, Shijia Zhuang 050024, People's Republic of China; and ruili@mail.hebtu.edu.cn guoyi@mail.hebtu.edu.cn.|Hebei Collaboration Innovation Center for Cell Signaling, Shijia Zhuang 050024, People's Republic of China ruili@mail.hebtu.edu.cn guoyi@mail.hebtu.edu.cn.
authors.forename: Yi
authors.identifier: 0000-0003-3119-7646
authors.initials: Y
authors.lastname: Guo
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D022181:COP-Coated Vesicles / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism* / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005298:Fertility; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006720:Homozygote; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D051479:Plant Infertility / Q000235:genetics; D030821:Plants, Genetically Modified; D011058:Pollen / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D053205:Pollen Tube / Q000254:growth & development / Q000648:ultrastructure; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C000623008:SEC31B protein, Arabidopsis; D033921:Vesicular Transport Proteins; D049452:Green Fluorescent Proteins