title: A DNA Circuit for IsomiR Detection.
abstract: A synthetic DNA oligonucleotide has been programmed to function as a biological circuit to detect 5'-IsomiRs. The circuit consists of two integrated DNA switches. The first is "activated" when a DNA probe is enzymatically modified by a reverse transcriptase that incorporates nucleotides complementary to the 5'-region of a microRNA (miRNA). Addition of the correct number and sequence of nucleotides enables the probe to assemble into an asymmetric DNA hairpin. The reconfigured hairpin probe then primes an internal polymerisation reaction, resulting in the synthesis of a symmetrical DNA hairpin. This activates the second switch, which then initiates the amplification of reverse-transcribed miRNA through a continuous cycle of DNA nicking and polymerisation. The DNA circuit enables sensitive and rapid detection of femtomoles of a miRNA transcript under isothermal conditions.
authors.affiliation: Flinders Centre for Nanoscale Science and Technology, Flinders University, Sturt Road, Bedford Park, SA, 5042, Australia.
authors.forename: Ashley R
authors.initials: AR
authors.lastname: Connolly
authors.affiliation: Australian Red Cross Blood Service, 17 O'Riordan Street, Alexandria, Sydney, NSW, 2015, Australia.
authors.forename: Rena
authors.initials: R
authors.lastname: Hirani
authors.affiliation: Flinders Centre for Nanoscale Science and Technology, Flinders University, Sturt Road, Bedford Park, SA, 5042, Australia.
authors.forename: Amanda V
authors.initials: AV
authors.lastname: Ellis
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology, School of Chemistry and Molecular Biosciences (SCMB), The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia.
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D024506:5' Flanking Region; D055029:Inverted Repeat Sequences; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D009841:Oligonucleotides
keywords: DNA; isothermal amplification; miRNA detection; molecular devices; nanotechnology