title: The evidence for open and closed exocytosis as the primary release mechanism.
abstract: Exocytosis is the fundamental process by which cells communicate with each other. The events that lead up to the fusion of a vesicle loaded with chemical messenger with the cell membrane were the subject of a Nobel Prize in 2013. However, the processes occurring after the initial formation of a fusion pore are very much still in debate. The release of chemical messenger has traditionally been thought to occur through full distention of the vesicle membrane, hence assuming exocytosis to be all or none. In contrast to the all or none hypothesis, here we discuss the evidence that during exocytosis the vesicle-membrane pore opens to release only a portion of the transmitter content during exocytosis and then close again. This open and closed exocytosis is distinct from kiss-and-run exocytosis, in that it appears to be the main content released during regular exocytosis. The evidence for this partial release via open and closed exocytosis is presented considering primarily the quantitative evidence obtained with amperometry.
authors.affiliation: Department of Chemistry and Chemical Engineering,Chalmers University of Technology,41296 Gothenburg,Sweden.
authors.forename: Lin
authors.initials: L
authors.lastname: Ren
authors.affiliation: Department of Chemistry and Chemical Biology,University of Gothenburg,41296 Gothenburg,Sweden.
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Mellander
authors.affiliation: Department of Chemistry and Chemical Engineering,Chalmers University of Technology,41296 Gothenburg,Sweden.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Keighron
authors.affiliation: Department of Chemistry and Chemical Engineering,Chalmers University of Technology,41296 Gothenburg,Sweden.
authors.forename: Ann-Sofie
authors.initials: AS
authors.lastname: Cans
authors.affiliation: Department of Chemistry and Chemical Engineering,Chalmers University of Technology,41296 Gothenburg,Sweden.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Kurczy
authors.affiliation: Département de Chimie,Ecole Normale Supérieure-PSL Research University,Sorbonne Universités-UPMC Univ. Paris 06,CNRS UMR 8640 PASTEUR,24 rue Lhomond,75005 Paris,France.
authors.forename: Irina
authors.initials: I
authors.lastname: Svir
authors.affiliation: Département de Chimie,Ecole Normale Supérieure-PSL Research University,Sorbonne Universités-UPMC Univ. Paris 06,CNRS UMR 8640 PASTEUR,24 rue Lhomond,75005 Paris,France.
authors.forename: Alexander
authors.initials: A
authors.lastname: Oleinick
authors.affiliation: Département de Chimie,Ecole Normale Supérieure-PSL Research University,Sorbonne Universités-UPMC Univ. Paris 06,CNRS UMR 8640 PASTEUR,24 rue Lhomond,75005 Paris,France.
authors.forename: Christian
authors.initials: C
authors.lastname: Amatore
authors.affiliation: Department of Chemistry and Chemical Engineering,Chalmers University of Technology,41296 Gothenburg,Sweden.
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Ewing