title: Performance of trichlorfon degradation by a novel Bacillus tequilensis strain PA F-3 and its proposed biodegradation pathway.
abstract: The novel trichlorfon (TCF)-degrading bacterium PA F-3, identified as Bacillus tequilensis, was isolated from pesticide-polluted soils by using an effective screening and domesticating procedure. The TCF biodegradation pathways of PA F-3 were also systematically elucidated. As revealed by high-performance liquid chromatography, the TCF residues in the mineral salt medium demonstrated that PA F-3 can utilize TCF as its sole carbon source and reach the highest degradation of 71.1 % at an initial TCF concentration of 200 mg/L within 5 days. The TCF degradation conditions were optimized using response surface methodology as follows: temperature, 28 °C; inoculum amount, 4 %; and initial TCF concentration, 125 mg/L. Biodegradation treatments supplemented with exogenous carbon sources and yeast extract markedly increased the microbial dry weights and TCF-degrading performance of PA F-3, respectively. Meanwhile, five metabolic products of TCF were identified through gas chromatography/mass spectrometry, and a biodegradation pathway was proposed. Results indicated that deoxidation and dehydration (including the cleavage of the P-C phosphonate bond and the C-O bond) were the preferred metabolic reactions of TCF in this TCF-degrading bacterium.
authors.affiliation: School of Resource and Environmental Sciences, Hubei Key Laboratory of Biomass-Resources Chemistry and Environmental Biotechnology, Wuhan University, Wuhan, 430079, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Tian
authors.affiliation: School of Resource and Environmental Sciences, Hubei Key Laboratory of Biomass-Resources Chemistry and Environmental Biotechnology, Wuhan University, Wuhan, 430079, People's Republic of China.
authors.forename: Chenlei
authors.initials: C
authors.lastname: Yu
authors.affiliation: School of Civil Engineering, Wuhan University, Wuhan, 430072, Hubei, People's Republic of China.
authors.forename: Yingwen
authors.initials: Y
authors.lastname: Xue
authors.affiliation: School of Resource and Environmental Sciences, Hubei Key Laboratory of Biomass-Resources Chemistry and Environmental Biotechnology, Wuhan University, Wuhan, 430079, People's Republic of China.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Zhao
authors.affiliation: School of Resource and Environmental Sciences, Hubei Key Laboratory of Biomass-Resources Chemistry and Environmental Biotechnology, Wuhan University, Wuhan, 430079, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Resource and Environmental Sciences, Hubei Key Laboratory of Biomass-Resources Chemistry and Environmental Biotechnology, Wuhan University, Wuhan, 430079, People's Republic of China. chenlz@whu.edu.cn.
authors.forename: Lanzhou
authors.initials: L
authors.lastname: Chen
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D001673:Biodegradation, Environmental; D007306:Insecticides / Q000378:metabolism*; D012988:Soil Microbiology; D014236:Trichlorfon / Q000378:metabolism*
chemical_list: D007306:Insecticides; D014236:Trichlorfon
keywords: Bacillus tequilensis; Biodegradation pathway; Biodegradation products; TCF degrading bacterium; Trichlorfon (TCF)