title: Characterization of a Wheat Breeders' Array suitable for high-throughput SNP genotyping of global accessions of hexaploid bread wheat (Triticum aestivum).
abstract: Targeted selection and inbreeding have resulted in a lack of genetic diversity in elite hexaploid bread wheat accessions. Reduced diversity can be a limiting factor in the breeding of high yielding varieties and crucially can mean reduced resilience in the face of changing climate and resource pressures. Recent technological advances have enabled the development of molecular markers for use in the assessment and utilization of genetic diversity in hexaploid wheat. Starting with a large collection of 819 571 previously characterized wheat markers, here we describe the identification of 35 143 single nucleotide polymorphism-based markers, which are highly suited to the genotyping of elite hexaploid wheat accessions. To assess their suitability, the markers have been validated using a commercial high-density Affymetrix Axiom®genotyping array (the Wheat Breeders' Array), in a high-throughput 384 microplate configuration, to characterize a diverse global collection of wheat accessions including landraces and elite lines derived from commercial breeding communities. We demonstrate that the Wheat Breeders' Array is also suitable for generating high-density genetic maps of previously uncharacterized populations and for characterizing novel genetic diversity produced by mutagenesis. To facilitate the use of the array by the wheat community, the markers, the associated sequence and the genotype information have been made available through the interactive web site 'CerealsDB'.
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Allen
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Mark O
authors.initials: MO
authors.lastname: Winfield
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Burridge
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.|The John Bingham Laboratory, NIAB, Cambridge, UK.
authors.forename: Rowena C
authors.initials: RC
authors.lastname: Downie
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Harriet R
authors.initials: HR
authors.lastname: Benbow
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Gary L A
authors.initials: GL
authors.lastname: Barker
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Wilkinson
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Jane
authors.initials: J
authors.lastname: Coghill
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Christy
authors.initials: C
authors.lastname: Waterfall
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Davassi
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Geoff
authors.initials: G
authors.lastname: Scopes
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Ali
authors.initials: A
authors.lastname: Pirani
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Teresa
authors.initials: T
authors.lastname: Webster
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Fiona
authors.initials: F
authors.lastname: Brew
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Claire
authors.initials: C
authors.lastname: Bloor
authors.affiliation: John Innes Centre, Norwich, Norfolk, UK.
authors.forename: Simon
authors.initials: S
authors.lastname: Griffiths
authors.affiliation: The John Bingham Laboratory, NIAB, Cambridge, UK.
authors.forename: Alison R
authors.initials: AR
authors.lastname: Bentley
authors.affiliation: RAGT Seeds, Ickleton, Essex, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Alda
authors.affiliation: RAGT Seeds, Ickleton, Essex, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Jack
authors.affiliation: Plant Biology and Crop Science Department, Rothamsted Research, Harpenden, UK.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Phillips
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Edwards
mesh_terms: D014644:Genetic Variation / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D005838:Genotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
keywords: genotyping array; single nucleotide polymorphism (SNP); wheat