title: Systematic Localization and Identification of SUMOylation Substrates in Knock-In Mice Expressing Affinity-Tagged SUMO1.
abstract: Protein SUMOylation is a posttranslational protein modification that is emerging as a key regulatory process in neurobiology. To date, however, SUMOylation in vivo has only been studied cursorily. Knock-in mice expressing His6-HA-SUMO1 from the Sumo1 locus allow for the highly specific localization and identification of endogenous SUMO1 substrates under physiological and pathophysiological conditions. By making use of the HA-tag and using wild-type mice for highly stringent negative control samples, SUMO1 targets can be specifically localized in and purified from cultured mouse nerve cells and mouse tissues.
authors.affiliation: Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Hermann-Rein-Straße 3, 37075, Göttingen, Germany. tirard@em.mpg.de.
authors.forename: Marilyn
authors.initials: M
authors.lastname: Tirard
authors.affiliation: Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Hermann-Rein-Straße 3, 37075, Göttingen, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Brose
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002846:Chromatography, Affinity; D000939:Epitopes / Q000235:genetics / Q000378:metabolism; D055879:Gene Knock-In Techniques; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000378:metabolism; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D047468:Immunoprecipitation; D051379:Mice; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism; D061251:Primary Cell Culture; D011499:Protein Processing, Post-Translational; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism*; D058207:Sumoylation; D016704:Synapsins / Q000235:genetics / Q000378:metabolism
chemical_list: D000906:Antibodies; D000939:Epitopes; D019267:Hemagglutinin Glycoproteins, Influenza Virus; C471213:His-His-His-His-His-His; D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; D009842:Oligopeptides; D025842:SUMO-1 Protein; D016704:Synapsins; C483468:hemagglutinin, human influenza A virus; D006639:Histidine
keywords: Affinity purification; HA-tag; Immunoprecipitation; In vivo; Knock-in mice; SUMOylation