title: Protective effect of cyanidin against glucose- and methylglyoxal-induced protein glycation and oxidative DNA damage.
abstract: Cyanidin, a natural anthocyanin abundant in fruits and vegetables, has shown the health benefits due to its pharmacological properties. However, there was no evidence regarding anti-glycation activity of cyanidin. The aim of the study was to investigate the inhibitory effect of cyanidin on methylglyoxal (MG)- and glucose-induced protein glycation in bovine serum albumin (BSA) as well as oxidative DNA damage. Free radical scavenging activity and the MG-trapping ability of cyanidin were also investigated. The results demonstrated that cyanidin (0.125-1mM) significantly inhibited the formation of fluorescent and non-fluorescent AGEs in BSA/MG and BSA/glucose systems. There was a significantly improved protein thiol in BSA/MG and BSA/glucose when incubated with cyanidin. Correspondingly, cyanidin decreased the level of protein carbonyl content in BSA/glucose system. Moreover, cyanidin (0.5-1mM) prevented lysine/MG-mediated oxidative DNA damage in the absence or presence of copper ion. The results demonstrated that cyanidin showed the MG-trapping ability in a concentration-dependent manner. Cyanidin also reduced superoxide anion and hydroxyl radical generation in lysine/MG system. The mechanism by which cyanidin inhibited protein glycation was the MG-trapping ability and the free radical scavenging activity. The present study suggests that cyanidin might be a promising antiglycation agent for preventing or ameliorating AGEs-mediated diabetic complications.
authors.affiliation: Program in Biomedical Sciences, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Tanyawan
authors.initials: T
authors.lastname: Suantawee
authors.affiliation: Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Henrique
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address: Sirichai.a@chula.ac.th.
authors.forename: Sirichai
authors.initials: S
authors.lastname: Adisakwattana
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000737:chemistry*; D002417:Cattle; D004249:DNA Damage; D005947:Glucose / Q000737:chemistry*; D006031:Glycosylation; D011765:Pyruvaldehyde / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D012710:Serum Albumin, Bovine; D011765:Pyruvaldehyde; C017154:cyanidin; D005947:Glucose
keywords: Advanced glycation end products; Cyanidin; Methylglyoxal