title: Leucocyte cytokines dominate platelet cytokines overtime in non-leucoreduced platelet components.
abstract: Leucoreduction of blood components, including platelet components, is strongly encouraged but not yet universal, especially outside high income countries. As both leucocytes and platelets secrete copious amounts of pro-inflammatory cytokines/chemokines under various conditions and during storage, we investigated the potential of the respective secretory programmes of these cells in order to evaluate their subsequent pathophysiological effects.
  
  
  
  A total of 158 individual non-leucoreduced platelet components were obtained from Tunisian donors and tested for characteristic biological response modifiers (BRM) of leukocytes (IL-1β, IL-8), platelets (sCD62P, sCD40L) and both cell types (TNF-α, RANTES) in the presence or absence of thrombin stimulation and after different periods of storage (up to 5 days). BRM levels were determined using enzyme-linked immunosorbent assays and Luminex technology. Platelet-leucocyte aggregate formation during storage was analysed using flow cytometry.
  
  
  
  Leucocyte- and platelet-associated BRM had clearly distinct profiles both at the onset (day 0) and termination (day 5) of the observation period but altered during the intermediate period so that their respective importance was inverted; in fact, the profiles were merged and indistinguishable on days 2-3. The leucocyte-derived BRM largely dominated over platelet-derived ones and further altered the BRM platelet secretion programme.
  
  
  
  This study contributes substantial, new information on leucocyte/platelet interactions and their likely role in transfusion when leucodepletion cannot be performed or is only partially achieved.
authors.affiliation: GIMAP-EA3064, University of Lyon, Saint-Etienne, France.|Auvergne-Loire French Blood Establishment, Saint-Etienne, France.
authors.forename: Chaker
authors.initials: C
authors.lastname: Aloui
authors.affiliation: Regional Blood Transfusion Centre, CHU Farhat Hached, Sousse, Tunisia.
authors.forename: Tahar
authors.initials: T
authors.lastname: Chakroun
authors.affiliation: GIMAP-EA3064, University of Lyon, Saint-Etienne, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Prigent
authors.affiliation: Regional Blood Transfusion Centre, CHU Farhat Hached, Sousse, Tunisia.
authors.forename: Saloua
authors.initials: S
authors.lastname: Jemni-Yacoub
authors.affiliation: GIMAP-EA3064, University of Lyon, Saint-Etienne, France.|Auvergne-Loire French Blood Establishment, Saint-Etienne, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Cognasse
authors.affiliation: GIMAP-EA3064, University of Lyon, Saint-Etienne, France.|Auvergne-Loire French Blood Establishment, Saint-Etienne, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Laradi
authors.affiliation: GIMAP-EA3064, University of Lyon, Saint-Etienne, France.|INTS - National Institute of Blood Transfusion, Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Garraud
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D001793:Blood Preservation; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D013997:Time Factors
chemical_list: D016207:Cytokines