title: Analysed cap mesenchyme track data from live imaging of mouse kidney development.
abstract: This article provides detailed information on manually tracked cap mesenchyme cells from timelapse imaging of multiple ex vivo embryonic mouse kidneys. Cells were imaged for up to 18 h at 15 or 20 min intervals, and multiple cell divisions were tracked. Positional data is supplemented with a range of information including the relative location of the closest ureteric tip and a correction for drift due to bulk movement and tip growth. A subset of tracks were annotated to indicate the presence of processes attached to the ureteric epithelium. The calculations used for drift correction are described, as are the main methods used in the analysis of this data for the purpose of describing cap cell motility. The outcomes of this analysis are discussed in "Cap mesenchyme cell swarming during kidney development is influenced by attraction, repulsion, and adhesion to the ureteric tip" (A.N. Combes, J.G. Lefevre, S. Wilson, N.A. Hamilton, M.H. Little, 2016) [1].
authors.affiliation: Division of Genomics of Development and Disease, Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: James G
authors.initials: JG
authors.lastname: Lefevre
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Melbourne, 3010 VIC, Australia; Murdoch Childrens Research Institute, Flemington Rd, Parkville, Melbourne, 3052 VIC, Australia.
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Combes
authors.affiliation: Murdoch Childrens Research Institute, Flemington Rd, Parkville, Melbourne, 3052 VIC, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, 3010 VIC, Australia.
authors.forename: Melissa H
authors.initials: MH
authors.lastname: Little
authors.affiliation: Division of Genomics of Development and Disease, Institute for Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia; Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Hamilton
keywords: Cap mesenchyme; Cell migration; Image analysis; Kidney development; Mathematical modelling