title: Chromosome mapping of repetitive DNAs in sergeant major fishes (Abudefdufinae, Pomacentridae): a general view on the chromosomal conservatism of the genus.
abstract: Species of the Abudefduf genus (sergeant-majors) are widely distributed in the Indian, Pacific and Atlantic oceans, with large schools inhabiting rocky coastal regions and coral reefs. This genus consists of twenty recognized species are of generalist habit, showing typical characteristics of colonizers. Some populations maintain gene flow between large oceanic areas, a condition that may influence their cytogenetic features. A number of species have been shown to be invaders and able to hybridize with local species. However, cytogenetic data in this genus are restricted to few species. In this way, the present study includes the chromosomal investigation, using conventional (Giemsa staining, Ag-NOR and C-banding) and molecular (in situ mapping of six different repetitive DNA classes) approaches in four Abudefduf species from different oceanic regions (A. bengalensis and A. sexfasciatus from the Indo-Pacific, A. vaigiensis from the Indian and A. saxatilis from the Atlantic oceans, respectively), to investigate the evolutionary events associated with the chromosomal diversification in this group. All species share a similar karyotype (2n = 48; NF = 52), except A. sexfasciatus (2n = 48; NF = 50), which possesses a characteristic pericentric inversion in the NOR-bearing chromosomal pair. Mapping of repetitive sequences suggests a chromosomal conservatism in this genus. The high karyotypic similarity between allopatric species of Abudefduf may be related to the success of natural viable hybrids among species with recent secondary contact.
authors.affiliation: Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen, Thailand.
authors.forename: Nuntaporn
authors.initials: N
authors.lastname: Getlekha
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, São Paulo, Brazil.
authors.forename: Marcelo de Bello
authors.initials: MB
authors.lastname: Cioffi
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, São Paulo, Brazil.
authors.forename: Cassia Fernanda
authors.initials: CF
authors.lastname: Yano
authors.affiliation: Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen, Thailand.
authors.forename: Nuntiya
authors.initials: N
authors.lastname: Maneechot
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, São Paulo, Brazil.
authors.forename: Luiz Antonio Carlos
authors.initials: LA
authors.lastname: Bertollo
authors.affiliation: Department of Fisheries, Faculty of Applied Science and Engineering, Khon Kaen University, Nong Khai Campus, Muang, Nong Khai, Thailand.
authors.forename: Weerayuth
authors.initials: W
authors.lastname: Supiwong
authors.affiliation: Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen, Thailand.|Toxic Substances in Livestock and Aquatic Animals Research Group, Khon Kaen University, Muang, Khon Kaen, 40002, Thailand.
authors.forename: Alongklod
authors.initials: A
authors.lastname: Tanomtong
authors.affiliation: Departamento de Biologia Celular e Genética, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil. molinawf@yahoo.com.br.
authors.forename: Wagner Franco
authors.initials: WF
authors.lastname: Molina
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes; D019143:Evolution, Molecular; D005260:Female; D005399:Fishes / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D059785:Karyotype; D008297:Male; D018895:Microsatellite Repeats; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D012337:RNA, Ribosomal, 18S
keywords: Chromosome evolution; Damselfishes; Pericentric inversions; Repetitive sequences; U2 snRNA