title: Effect of short-term exposure of cumulus-oocyte complex to 3-morpholinosydnonimine on in vitro embryo development and gene expression in cattle.
abstract: Short-term exposure of gametes to different types of stress might induce stress tolerance in mammalian embryos. The aim of this study was to evaluate the effect of short-term exposure of bovine mature cumulus-oocyte complex (COC) to 3-morpholinosydnonimine (SIN-1) on subsequent in vitro embryo development, embryo quality and relative gene expression. Matured COCs were incubated with SIN-1 (0, 0.1, 1, 10 and 100 μM SIN-1) for 1 hr before in vitro fertilization and zygotes were cultured until Day 7. The cleavage rate at 72 hr did not show any differences among groups. However, the blastocyst rate on Day 7 decreased with all treatments evaluated, with the embryos generated with 10 μM SIN-1 showing the lowest embryo production rate. Embryo quality analysis did not show any differences in total cell number (TCN) or inner cell mass (ICM) among groups. Relative gene expression analysis showed a downregulation of eNOS expression and an upregulation of nNOS expression in all treatments evaluated compared to the control group. Also, a downregulation was observed in some treatments: SOD2 at 0.1 μM; SOD1 at 0.1 and 100 μM; PRDX5 at 0.1, 10 and 100 μM; and NANOG at 10 and 100 μM; and an upregulation of CDX2 expression was observed at 100 μM. The other genes (OCT4, HIF1A, HSPA1A, BCL2A and iNOS) did not show any differences in the relative gene expression. These results suggest that the short-term exposure of mature bovine COCs to SIN-1 does not induce stress tolerance and has no beneficial effect on bovine in vitro embryo production.
authors.affiliation: Student of Doctoral Program in Sciences major in Applied Cellular and Molecular Biology, Universidad de la Frontera, Temuco, Chile.|Centre of Biotechnology on Reproduction (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.
authors.forename: P
authors.initials: P
authors.lastname: Loren
authors.affiliation: Centre of Biotechnology on Reproduction (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.
authors.forename: C
authors.initials: C
authors.lastname: Cheuquemán
authors.affiliation: Student of Doctoral Program in Sciences major in Applied Cellular and Molecular Biology, Universidad de la Frontera, Temuco, Chile.|Centre of Biotechnology on Reproduction (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.
authors.forename: E
authors.initials: E
authors.lastname: Sánchez
authors.affiliation: Centre of Biotechnology on Reproduction (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.|Department of Basic Science, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.
authors.forename: J
authors.initials: J
authors.lastname: Risopatrón
authors.affiliation: Centre of Biotechnology on Reproduction (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.|Department of Agricultural and Livestock Production, Faculty of Farming, Livestock and Forestry Sciences, Universidad de La Frontera, Temuco, Chile.
authors.forename: M E
authors.initials: ME
authors.lastname: Arias
authors.affiliation: Centre of Biotechnology on Reproduction (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.|Department of Agricultural and Livestock Production, Faculty of Farming, Livestock and Forestry Sciences, Universidad de La Frontera, Temuco, Chile.
authors.forename: R
authors.initials: R
authors.lastname: Felmer
authors.affiliation: Centre of Biotechnology on Reproduction (CEBIOR-BIOREN), Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.|Department of Preclinical Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile.
authors.forename: R
authors.initials: R
authors.lastname: Sánchez
mesh_terms: D000818:Animals; D002417:Cattle / Q000196:embryology*; D054885:Cumulus Cells / Q000502:physiology*; D046149:Embryo Culture Techniques / Q000662:veterinary*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects* / Q000502:physiology; D008981:Molsidomine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009865:Oocytes / Q000502:physiology*
chemical_list: C002385:linsidomine; D008981:Molsidomine