title: Advances in the molecular diagnosis of diffuse large B-cell lymphoma in the era of precision medicine.
abstract: The adoption of high-throughput technologies has led to a transformation in our ability to classify diffuse large B-cell lymphoma (DLBCL) into unique molecular subtypes. In parallel, the expansion of agents targeting key genetic and gene expression signatures has led to an unprecedented opportunity to personalize cancer therapies, paving the way for precision medicine. Areas covered: This review summarizes the key molecular subtypes of DLBCL and outlines the novel technology platforms in development to discriminate clinically relevant subtypes. Expert commentary: The application of emerging diagnostic tests into routine clinical practise is gaining momentum following the demonstration of subtype specific activity by novel agents. Co-ordinated efforts are required to ensure that these state of the art technologies provide reliable and clinically meaningful results accessible to the wider haematology community.
authors.affiliation: a Centre for Haemato-Oncology , Barts Cancer Institute, Queen Mary University of London , London , UK.
authors.forename: Shamzah
authors.initials: S
authors.lastname: Araf
authors.affiliation: a Centre for Haemato-Oncology , Barts Cancer Institute, Queen Mary University of London , London , UK.
authors.forename: Koorosh
authors.initials: K
authors.lastname: Korfi
authors.affiliation: a Centre for Haemato-Oncology , Barts Cancer Institute, Queen Mary University of London , London , UK.
authors.forename: Tahrima
authors.initials: T
authors.lastname: Rahim
authors.affiliation: b Cancer Sciences Unit, Faculty of Medicine , University of Southampton , Southampton , UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Davies
authors.affiliation: a Centre for Haemato-Oncology , Barts Cancer Institute, Queen Mary University of London , London , UK.
authors.forename: Jude
authors.initials: J
authors.lastname: Fitzgibbon
mesh_terms: D014408:Biomarkers, Tumor; D015870:Gene Expression; D019254:Genes, bcl-2; D016259:Genes, myc; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis* / Q000235:genetics* / Q000401:mortality / Q000628:therapy; D025202:Molecular Diagnostic Techniques; D009154:Mutation; D057285:Precision Medicine / Q000379:methods; D011379:Prognosis; D059467:Transcriptome
chemical_list: D014408:Biomarkers, Tumor
keywords: BCL2; Diffuse large B-cell lymphoma (DLBCL); MYC; cell of origin (COO); precision medicine; targeted sequencing