title: Association of TNFRSF1B +676 gene polymorphism with the risk of rheumatoid arthritis in Han Chinese population in Hunan.
abstract: OBJECTIVE
  To study the association of TNFRSF1B +676 gene (rs1061622) polymorphism with the risk of rheumatoid arthritis (RA ) in Han Chinese population of Hunan.
  
  
  METHODS
  A total of 112 patients with RA from Han Chinese population in Hunan were recruited, along with 129 healthy controls. TNFRSF1B +676 (rs1061622) gene polymorphisms were examined by PCR-RFLP. Serum levels of soluble TNFR II were analyzed by ELISA.
  
  
  RESULTS
  RA patients displayed a similar TNFRSF1B +676 genotype to controls (GG/TG/TT: 5/62/45 vs 9/56/64, P=0.167), but signifi cant diff erence was found between female RA patients and female controls (GG/TG/TT: 3/49/24 vs 8/28/48, P<0.001). No significant difference was found in the frequency of TNFRSF1B +676 T or G allele between RA patients and controls (P>0.05). RA patients showed a signifi cantly higher level of serum soluble tumor necrosis factor receptor II (sTNFR II) than controls [(7.83±2.61) ng/mL vs (4.32±1.67) ng/mL, P<0.001], but there was no diff erence among the three genotypes (P>0.05). No association was found between TNFRSF1B+676 gene polymorphism and RA clinical characteristics.
  
  
  CONCLUSIONS
  In Han Chinese population of Hunan province, TNFRSF1B+676 gene polymorphisms are not associated with the genetic risk of RA .
authors.affiliation: Department of Rheumatology and Immunology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Rheumatology and Immunology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Rheumatology and Immunology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Jinwei
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Rheumatology and Immunology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Jiesheng
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Gastroenterology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Fanggen
authors.initials: F
authors.lastname: Lu
mesh_terms: D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000208:ethnology / Q000235:genetics*; D044466:Asian People; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000097:blood / Q000235:genetics*; D012307:Risk Factors
chemical_list: D047889:Receptors, Tumor Necrosis Factor, Type II; C415902:TNFRSF1B protein, human