title: Metabolic switches during the first steps of adipogenic stem cells differentiation.
abstract: The understanding of metabolism during cell proliferation and commitment provides a greater insight into the basic biology of cells, allowing future applications. Here we evaluated the energy and oxidative changes during the early adipogenic differentiation of human adipose tissue-derived stromal cells (hASCs). hASCs were maintained under differentiation conditions during 3 and 7days. Oxygen consumption, mitochondrial mass and membrane potential, reactive oxygen species (ROS) generation, superoxide dismutase (SOD) and catalase activities, non-protein thiols (NPT) concentration and lipid peroxidation were analyzed. We observed that 7days of adipogenic induction are required to stimulate cells to consume more oxygen and increase mitochondrial activity, indicating organelle maturation and a transition from glycolytic to oxidative energy metabolism. ROS production was only increased after 3days and may be involved in the differentiation commitment. ROS source was not only the mitochondria and we suggest that NOX proteins are related to ROS generation and therefore adipogenic commitment. ROS production did not change after 7days, but an increased activity of catalase and NPT concentration as well as a decreased lipid peroxidation were observed. Thus, a short period of differentiation induction is able to change the energetic and oxidative metabolic profile of hASCs and stimulate cytoprotection processes.
authors.affiliation: Laboratório de Biologia Básica de Células Tronco, Instituto Carlos Chagas, Fiocruz, Curitiba, Paraná, Brazil.
authors.forename: Daiana Leila
authors.initials: DL
authors.lastname: Drehmer
authors.affiliation: Laboratório de Biologia Básica de Células Tronco, Instituto Carlos Chagas, Fiocruz, Curitiba, Paraná, Brazil.
authors.forename: Alessandra Melo
authors.initials: AM
authors.lastname: de Aguiar
authors.affiliation: Universidade Federal do Paraná, Paraná, Paraná, Brazil.
authors.forename: Anna Paula
authors.initials: AP
authors.lastname: Brandt
authors.affiliation: Universidade Federal do Paraná, Paraná, Paraná, Brazil.
authors.forename: Lyvia
authors.initials: L
authors.lastname: Petiz
authors.affiliation: Universidade Federal do Paraná, Paraná, Paraná, Brazil.
authors.forename: Sílvia Maria Suter Correia
authors.initials: SM
authors.lastname: Cadena
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: Carmen K
authors.initials: CK
authors.lastname: Rebelatto
authors.affiliation: Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
authors.forename: Paulo R S
authors.initials: PR
authors.lastname: Brofman
authors.affiliation: Universidade Federal do Paraná, Paraná, Paraná, Brazil.
authors.forename: Francisco
authors.initials: F
authors.lastname: Filipak Neto
authors.affiliation: Laboratório de Biologia Básica de Células Tronco, Instituto Carlos Chagas, Fiocruz, Curitiba, Paraná, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Dallagiovanna
authors.affiliation: Laboratório de Biologia Básica de Células Tronco, Instituto Carlos Chagas, Fiocruz, Curitiba, Paraná, Brazil. Electronic address: ana.abud@fiocruz.br.
authors.forename: Ana Paula Ressetti
authors.initials: AP
authors.lastname: Abud
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D006019:Glycolysis; D006801:Humans; D015227:Lipid Peroxidation; D053078:Membrane Potential, Mitochondrial; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D002374:Catalase; D013482:Superoxide Dismutase; D019255:NADPH Oxidases
keywords: Adipogenesis; Adult stem cells; Antioxidant activity; Cytoprotection; Metabolism; Reactive oxygen species (ROS)