title: Potential Nociceptive Regulatory Effect of Probiotic Lactobacillus rhamnosus PB01 (DSM 14870) on Mechanical Sensitivity in Diet-Induced Obesity Model.
abstract: Treatments for obesity have been shown to reduce pain secondary to weight loss. Intestinal microbiota, as an endogenous factor, influences obesity and pain sensitivity but the effect of oral probiotic supplementation on musculoskeletal pain perception has not been studied systematically. The present study examined the effect of a single daily oral dose (1 × 10(9) CFU) of probiotics (Lactobacillus rhamnosus PB01, DSM14870) supplement on mechanical pain thresholds in behaving diet-induced obese (DIO) mice and their normal weight (NW) controls. The mice (N = 24, 6-week-old male) were randomly divided into four groups on either standard or high fat diet with and without probiotic supplementation. Both DIO and NW groups with probiotic supplementation maintained an insignificant weight gain while the control groups gained significant weight (P < 0.05). Similarly, both DIO and NW probiotics supplemented groups demonstrated a significantly (P < 0.05) lower sensitivity to mechanical stimulation compared to their corresponding control. The results of this study suggest a protective effect of probiotics on nociception circuits, which propose a direct result of the weight reduction or an indirect result of anti-inflammatory properties of the probiotics. Deciphering the exact underlying mechanism of the weight loss and lowering nociception effect of the probiotic applied in this study require further investigation.
authors.affiliation: Biomedicine Group, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark; SMI®, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark.
authors.forename: Fereshteh
authors.identifier: 0000-0002-2034-0147
authors.initials: F
authors.lastname: Dardmeh
authors.affiliation: Biomedicine Group, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark.
authors.forename: Hans Ingolf
authors.identifier: 0000-0003-4690-1501
authors.initials: HI
authors.lastname: Nielsen
authors.affiliation: Biomedicine Group, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark.
authors.forename: Hiva
authors.identifier: 0000-0002-8500-4208
authors.initials: H
authors.lastname: Alipour
authors.affiliation: Department of Clinical Medicine, The Faculty of Medicine, Aalborg University Hospital, Aalborg, Denmark.
authors.forename: Benedict
authors.identifier: 0000-0001-5797-7638
authors.initials: B
authors.lastname: Kjærgaard
authors.affiliation: Bifodan A/S, Hundested, Denmark.
authors.forename: Erik
authors.initials: E
authors.lastname: Brandsborg
authors.affiliation: Biomedicine Group, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark; SMI®, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark.
authors.forename: Parisa
authors.identifier: 0000-0003-0109-3600
authors.initials: P
authors.lastname: Gazerani
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D052201:Lacticaseibacillus rhamnosus; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D059226:Nociceptive Pain / Q000178:diet therapy*; D009765:Obesity / Q000150:complications / Q000178:diet therapy / Q000209:etiology; D017288:Pain Threshold / Q000187:drug effects*; D010812:Physical Stimulation / Q000009:adverse effects; D019936:Probiotics / Q000627:therapeutic use*
chemical_list: D008055:Lipids